LOCUS       DAONVQ010000001        60682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_258_length_60682_cov_4.846916, whole genome shotgun sequence.
ACCESSION   DAONVQ010000001 DAONVQ010000000
VERSION     DAONVQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 60682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60682
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_258_length_60682_cov_4.846916"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLD98_00005"
     CDS             complement(<1..251)
                     /locus_tag="PLD98_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04675.1"
                     /translation="MRRRRKLKFTTEKNLKAKRVKAFVISFVCFVLLFGSVSFLMLLH
                     SLDYDLSNLVGGGDITNTTEEQEETTSITDALHGTKTFFV"
     gene            complement(347..613)
                     /locus_tag="PLD98_00010"
     CDS             complement(347..613)
                     /locus_tag="PLD98_00010"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HQA04676.1"
                     /translation="MKPFFYYIALIGTISAILTLYDKIAAIGRKRRIPESSLLWIALL
                     GGALPMYLVMIVIRHKTRHKKFMILLPLMAVLHTAIVCFAYFLR"
     gene            complement(677..1792)
                     /locus_tag="PLD98_00015"
     CDS             complement(677..1792)
                     /locus_tag="PLD98_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HQA04677.1"
                     /translation="MDINLLPTLLKKFFAMLTVLSLLFAGIGGTGTETTETEIKNVIF
                     FIGDGMGPNHLEKTKAETGEELFMDTLPVQGFSKTSNYTGGVTDSAAGATALACGIRT
                     ANGRVGVYITDPFAYASYPMNLTELCMERGMKTGIVTSDSTAGATPAGFSAHVFQRKY
                     TEDITKQQLASGIDLIWGLSTGLVTPEIVAETGYKYVESMEDILALEDGDKSFGQFTN
                     ELWRLAGGSSYPTLSQLTEAAIDLLKNENGFFLMVEGAHIDKHSHSNKAEEMKEALLE
                     FDKAIQIATEFAMAEGNTLIVVTADHETGAIKYKNGAYEFTSGSHSGANVPLRVYGCN
                     DFIKPGQAIKNREIPVRVAKALGFAESAFPRTIDAAA"
     gene            complement(1907..2206)
                     /locus_tag="PLD98_00020"
     CDS             complement(1907..2206)
                     /locus_tag="PLD98_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04678.1"
                     /translation="MLFKKRKKDKKQIDRFQKLVAGGVEIKALNCRVFPEDDIAVLEI
                     TVPEVTEALHYDELDFELYTSCKVIKMKGEFKDAEKKGKLRFYRYKLYEYNEFFE"
     gene            complement(2223..3149)
                     /locus_tag="PLD98_00025"
     CDS             complement(2223..3149)
                     /locus_tag="PLD98_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQA04679.1"
                     /translation="MKQGSKKKNGGKNKRLRLAIFALCLVALTLVTGVFVYKNKDAIS
                     NRKHEYYYQLRLNLDRIFTEVRLKERDESRYTLKEYTLSEIKKDPNIHYDNSLILINS
                     EHKVYEGADEYVGEYKDKNVFMHKAIMKPYEELAEYINKKYGKKLYISSAYRTREKQE
                     ELAASKSKDTAAAPDASEHRTGLALDVYVNNYAGEAFIKSREGVYVNLNAYKYGLIIR
                     YPENKTEITGIKFEPWHLRYVGVPHAEIMYRNDLCLEEYIESLKVGVFYEANGYIISR
                     QSGESLKIPQNMKSVRVSPDNTGCYIITAEKP"
     gene            complement(3176..3634)
                     /gene="nrdR"
                     /locus_tag="PLD98_00030"
     CDS             complement(3176..3634)
                     /gene="nrdR"
                     /locus_tag="PLD98_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQA04680.1"
                     /translation="MKCPYCSFEESKVIDSRPTDEGERIRRRRECMKCAKRFTTYEII
                     ESVPIVVIKKDKSREVFDRDKLLNGMLKACEKRPVSISRLEQAVDEIVAEIHNSLDRE
                     ITSRRIGELAMCKLKDIDEVAYVRFASVYRQFKDINTFMEELTKLLSEKC"
     gene            complement(3719..4234)
                     /locus_tag="PLD98_00035"
     CDS             complement(3719..4234)
                     /locus_tag="PLD98_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQA04681.1"
                     /translation="MITVVSALLHEAGHLLCLISYDAPPSEIIIGAAGFKMKMSGKIL
                     SYRQETVVSLAGVFVNFLIASLLSIFYCFAGGDNRLLMPIYANLGLGVVNLLPIEPLD
                     GGRALYFWLCQKRTEAHAGRITDKVGLAFLMPLFILSFIILVKSGYNITLLCISIYLG
                     ISLIARAGAKR"
     gene            complement(4327..5199)
                     /locus_tag="PLD98_00040"
     CDS             complement(4327..5199)
                     /locus_tag="PLD98_00040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQA04682.1"
                     /translation="MNRLKNRKLKPKNKGTDFFVRLTLVQIIVAAVIMLVFLGAFKLN
                     KNIFGGMSGEYSRLMKEDMGEEYANSIKSAVARLAGKEPPAEEETAEADESDEVVDEK
                     DVYRDAYETDEAGNIISEDEWVTKPGAETAPDDDEPIGGGEDMKEVDGEIVSVVFKPY
                     TLGIKLIYPVDGYITSGFGFREHPITKEWGFHTGIDIGAMEGTEIKSAYDGTVCETGS
                     TKGRGNYIVVDHGGGIKTLYAHCLELLKSEGDKVKKGEAIALVGSTGLSTGNHVHFEV
                     QIDGIVRDPKYALK"
     gene            5340..6383
                     /locus_tag="PLD98_00045"
     CDS             5340..6383
                     /locus_tag="PLD98_00045"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HQA04683.1"
                     /translation="MSIEKRLFGKTPQGEEVFEFVLANDNAEVSLISRGAALRRLLVA
                     DKDGEKRDVLLGFDDLEGYIERSDYQGVAVGPFANRIEGGRLKIGGKEIIVPPNEKGR
                     NLLHSGGLYSNAVWSGEVSGDNKVVFKKRFKDMEGGFPGNTDAEISYTLESDGTVLME
                     YKAVSDSDMYFNLTNHAYFNLAGYDGGDILSHKLKINADSFLPVDENSIPTGESRKVE
                     GTPFDFREFKEIGRDIEADYEQLKLTGGYDHCFCLNDFGKGCRLAASVKCEKSGLCLD
                     VFTDLPGVQLYTGNFLSGTVGKGGKPMNHRSGFCLETQYYPDSPNKPQFPSCYVKAGE
                     VFFTKTSYRVYRY"
     gene            6692..6910
                     /locus_tag="PLD98_00050"
     CDS             6692..6910
                     /locus_tag="PLD98_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009983186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HQA04684.1"
                     /translation="MKIEINSNLCKGCEFCIAFCPKKVLKMQSERGPKGYFIPYLTDN
                     DGCTSCGICATMCPDGAIEVFKEVETDG"
     gene            6903..7973
                     /gene="vorB"
                     /locus_tag="PLD98_00055"
     CDS             6903..7973
                     /gene="vorB"
                     /locus_tag="PLD98_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HQA04685.1"
                     /translation="MAKVLMKGCEAVAEAAVRAGCRFFAGYPITPQNEIPEYLSRRLP
                     EVGGVFIQGESEVASVNMVYGAATMGVRAMTSSSGPGISLKAEGISYLASCKLPALIV
                     NVSRGGPGLGSIQPAQSDYLQATKALGHGGFRVMVFAPYTLQEAVDLTYQAFDYAERD
                     RNPVFLLMDGCLGAIMELVELPEMKEPSDENTKKWNLRKRPDEKRPARALTPIYPEII
                     LEMENKTRGELYKKWEREDVKVEEFMIDDAEYVVVAYGIAARVAKEAVITLREQGYKI
                     GMIRPITLYPFPKQSFHKLNYDKVKAIIDIELTIPAQMRDDIALEVMGRAPIFEYGRS
                     GGNLLDDDGTYDAIMKVIKEVG"
     gene            7973..8731
                     /locus_tag="PLD98_00060"
     CDS             7973..8731
                     /locus_tag="PLD98_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQA04686.1"
                     /translation="MAKEYKKIYSRPYAIMPVMSGYCPGCLHSLATKIIAEAVEELGQ
                     QENAMHILPVGCSALGLLYWRGDMVGAAHGRAPAVATGIKRCCPDRLVFAYQGDGDLA
                     AIGLAEIISAANRGENFTVIFANNSTYGMTGGQMAPTTLIGQKSTTTSEGRRVEDAGY
                     PIKMAEIIAQLEAPAYVARFALNTPKGVNEARKGIKKAFRMQLEGKGFTFIELLTNCP
                     TNWGMSPLKSLQYMTENTMKYFVPGVYKDVEVGA"
     gene            8728..9279
                     /locus_tag="PLD98_00065"
     CDS             8728..9279
                     /locus_tag="PLD98_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQA04687.1"
                     /translation="MKQSILISGSGGQGVLSAGMLLAQSAADSGKYASFLPEYGPEQR
                     GGSAKCTVLTSDKEIISPLPKKCMYWICMNEQSFKKFGTQLKEGGVLVYNSNRVTSPI
                     TRTDITAIPVPSDQLADQLGNPKVANIILIGALLAARKAIVDPEHFLKSLEEKFASKS
                     DEIRELNAKAFKIGGDIGKDYAE"
     gene            9309..9524
                     /locus_tag="PLD98_00070"
     CDS             9309..9524
                     /locus_tag="PLD98_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04688.1"
                     /translation="MPKYSFENTIGELLDTPETRALIDELCPELLEHPMLEVGRPFKV
                     NRALPFIEGLADKERIENFRRRLEAIE"
     gene            complement(9752..10681)
                     /gene="cysK"
                     /locus_tag="PLD98_00075"
     CDS             complement(9752..10681)
                     /gene="cysK"
                     /locus_tag="PLD98_00075"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQA04689.1"
                     /translation="MADIKKGFLDLVGNTPIAELSKFAEKLGAKARILAKLEYFNPAG
                     SVKDRIAKAMIEDAEQRGVLKPGAVIIEPTSGNTGIGLAAVAASKGYRIILTMPDTMS
                     IERRNLLKAYGAELVLTEGALGMKGAIAKAEELAKEIPGSFIPGQFDNPANPAIHKAT
                     TAVEIWNQTDGKVDIFVAGVGTGGTITGVGEFLKEKNPNIKVVAVEPSASPVLSEGKA
                     GPHKIQGIGAGFVPNTLNTSVYDEVIKVDNEDAFATGKEIALTEGILVGISSGAAAWA
                     AVQLSKRPENEGKNIVVILPDTGDRYLSTPLFS"
     gene            complement(10929..11222)
                     /locus_tag="PLD98_00080"
     CDS             complement(10929..11222)
                     /locus_tag="PLD98_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04690.1"
                     /translation="MAEKKKEKLLTYKGKPLVRCGRTLYYGNPEDPFVIMLQILETKQ
                     MGDLELADKVSVQLLNTDPEVRPKDRIVKKSEKKGLYEAMDIASIWLERALNP"
     gene            complement(11254..11514)
                     /locus_tag="PLD98_00085"
     CDS             complement(11254..11514)
                     /locus_tag="PLD98_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HQA04691.1"
                     /translation="MTDKTITFSIRDEYDMEMKKILAQVYRALEEKGYNPISQLVGYL
                     LSEDPTYITTHNNARNLIRRLDRDEILQAMIKDYLGIVKGKP"
     gene            complement(11641..13020)
                     /locus_tag="PLD98_00090"
     CDS             complement(11641..13020)
                     /locus_tag="PLD98_00090"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HQA04692.1"
                     /translation="MLNSEKSMEKIVALCKGRGFVYAGSEIYGGLSNTWDYGPLGVEF
                     KNNVKSAWWQKFVRECPYNVGLDTAILMNPTVWVASGHVGGFSDPLMDCKECKTRHRA
                     DKLIEDSGANPAGWSFEEMSKYIVEHNIVCPNCGALNFTEIRKFNLMFKTFQGVTEDS
                     KNEIYLRPETAQGIFVNFLNVQRTARKKVPFGVCQIGKSFRNEITPGNFIFRTREFEQ
                     MELEFFCKPGTELEWFSYWKDYCVNWLKSLGIKDENLKLRDHETEELSHYSNATTDIE
                     FMFPFGWGELWGIASRTDFDLTQHQNTSGKSLEYFDQETNEKYLPYVVEPSLGVDRVA
                     LAFLCEAYDEEVVDAEKGDVRVVLRLHPALAPVKAAVLPLSKKLGEQAEKIYNELSKS
                     FNVEYDDAGSIGKRYRRQDEIGTPFCITYDFDSENDGCVTVRDRDSMEQVRIPIASLK
                     DYIAEKLVF"
     gene            complement(13202..14329)
                     /gene="hemW"
                     /locus_tag="PLD98_00095"
     CDS             complement(13202..14329)
                     /gene="hemW"
                     /locus_tag="PLD98_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQA04693.1"
                     /translation="MNSKDKKGIYVHIPFCKSKCHYCDFCSVVPSGDIIDRYVDALKR
                     EAEGYKRKNKIKADTVYFGGGTPSFIGAERLSDILLHLRKVFDISPDAEITAECNPSS
                     CGGDFFSRLIDSGFNRISLGLQSADPRERKAIGRLTDADAAKQAALNAKKAGFNNISL
                     DLMLGLPFQNTESLKNSIDFCADAGVTHVSAYILKAEEGTPFYEKLSIEGAKKMNLPD
                     EDAVCDLYLFAAEYLQEKGFEQYEISNFCKKGHISRHNYKYWNLDEYIGLGVSAHSFF
                     EGKRFFNTRDIDEYIKGSKPIPDGSGGDFEEYLMLRLRLAEGLIRHDTERRFGKGCFE
                     KVLDKAKKYKGTNLLNISEDSISLTKQGFLVSNTIIAELSS"
     gene            complement(14319..16127)
                     /gene="lepA"
                     /locus_tag="PLD98_00100"
     CDS             complement(14319..16127)
                     /gene="lepA"
                     /locus_tag="PLD98_00100"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQA04694.1"
                     /translation="MSNKEFIRNFSIIAHIDHGKSTLADRLLELTNSVQSREMTQQLL
                     DSMDLERERGITIKAHAVRLEYKAKDGNDYVLNLIDTPGHVDFNYEVSRSLAACEGAV
                     LIVDATQGIEAQTMANTYLALDNNLELVPVINKIDLPAADPDRVARDIEEFIGLDCSE
                     APRISAKTGENVEAVLENIVKLVPPPKGDENAPLKALIFDSYYDSYKGVVVYVRIMDG
                     SIKPGDTMKMMSTGATFTVVETGYMRPTSLQPCKGLSAGEVGYITASIKTVSQASVGD
                     TITLVNNPAKEPLAGYRKANPMVFCGIYPADGADYESLKEALEKLRLNDAALSFEPET
                     SGALGFGFRCGFLGLLHLEIVQERLEREYDLDLISTVPAVIYKVHLRDGRVIEVDNPT
                     HFPDPADIDFIEEPIARAHIFSPPDYVGAIMELCQNRRGVYLDMVYITPERVDISYIL
                     PLNEIIYDFFDALKSRTKGYASLDYELAGYQQSKIVKLDVLLNGEVIDALSFMVHEEK
                     AYQRARRIAEKLKENIPRHMFEVPIQVAIGGKVIARETVKALRKDVLAKCYGGDITRK
                     KKLLEKQKEGKKRMRQFGSVEVPQEAFLAVLKLDEQ"
     gene            complement(16152..17057)
                     /locus_tag="PLD98_00105"
     CDS             complement(16152..17057)
                     /locus_tag="PLD98_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HQA04695.1"
                     /translation="MSEKTIKKRTNSTPKDPMRQLVADAFFFIVGSALYAISVNCFTA
                     PNHIAPGGVTGLATVGNYLFGLPIGTTVILLNIPLFILSLIFFGKKFLFRTVVATVLA
                     SVVIDITAGFLPVYSGDKLLAALFGGVFSGAGLGLVFIRGATTGGVDIIGKLMKLKFP
                     HISMGSFMLVLDMMVVIVAGIAYKSIESVLYAIIAFYISSKAIDYLLYGTTNNKMIQI
                     VTDKGREIAKAITNETSRGVTILPVTGGYTGKERSMLLCVTRANEVALIMRIARSIDE
                     NPFIIVSQAGDVFGLGFKPHRGDVI"
     gene            complement(17023..17541)
                     /locus_tag="PLD98_00110"
     CDS             complement(17023..17541)
                     /locus_tag="PLD98_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HQA04696.1"
                     /translation="MFLNRKNKKTNALEPEKAEDKSINLPELSLNPDAFKLKKSPREM
                     STYSLAFMGDAVYEALARSYIVANSQSAVGRLHLDKLEIVNASAQFRAAKVIFDYLTE
                     DERQIFLRGRNAHNAHIPKNKSAEEYRYATALEALFGYLYLSGKTDRLYILFDIIMRD
                     INERKDNKKENE"
     gene            complement(17531..18802)
                     /gene="obgE"
                     /locus_tag="PLD98_00115"
     CDS             complement(17531..18802)
                     /gene="obgE"
                     /locus_tag="PLD98_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQA04697.1"
                     /translation="MFIDSAKIKVKAGNGGNGAVAFHREKYVPAGGPDGGDGGKGGDV
                     VFVVDDNLSTLYDFRHKKKFIAQSGENGSGNKRNGKSAPDLIIKVPRGTIIKEAETGA
                     LIADMSKIERFVAAKGGRGGFGNARFATSTRQAPRFAREGTPGEEWELILELKLLADV
                     GLIGFPNVGKSTLLSLVSAAKPVIADYHFTTITPVLGVVSVGDKSFVMADIPGLIEGA
                     GEGVGLGHTFLRHIERCRLLVHIVDVSGSEGRDPVEDFDTINAELATFNKELLNKPQI
                     VVGNKIDLAEDEQIERLRKKAEECGYPFFTMSAAIAHGTQELINCIAAMLDKLPPIKI
                     YESEEVPLEALRKKGESEGFNIRQQNGIYIIEAEWLLKILNKTNLDDYESLQYFQRVL
                     QSSGIIAELERMGIQEGDTVKIYDLEFDYVP"
     gene            complement(18914..19144)
                     /locus_tag="PLD98_00120"
     CDS             complement(18914..19144)
                     /locus_tag="PLD98_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04698.1"
                     /translation="MYKRIKKLKNPDISRMGAVSFFDATGLYQTPPKNRFEEESYAHI
                     VDAVSDIDSDEDDLSDYYGDEYLNSLYKKKFS"
     gene            complement(19909..21141)
                     /gene="tyrS"
                     /locus_tag="PLD98_00125"
     CDS             complement(19909..21141)
                     /gene="tyrS"
                     /locus_tag="PLD98_00125"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQA04699.1"
                     /translation="MMNVFDELMARGIIAQMTDEEKIKELLTKDKIRFYIGFDPTADS
                     LHVGHFVQIMVISHLQKAGHTPIILFGGGTGLIGDPSGKTEMRKMLSKEEIQHNIECF
                     KTQMSRLVDFSDGKAILVNNADWLLKLNYVDMLRDVGVHFSVNRMLAAECYKQRLERG
                     LSFFELNYMIMQSYDFLHLRRHYNCIMQLGGDDQWSNIIGGVELNRRADGAEVYGMTF
                     KLLLTSDGRKMGKTEKGALWLDPEKTSPYEFFQYWRNVDDSDVINCLKMLTFVPVEQI
                     AEYEKYEGSELNKVKEVLAYEVTALVHGKDEAQKALEASRALFQKGVSDANMPTTELT
                     EDLFVDGKISVADLLVATKLAPSKGEARRLIQQGGITVDDEKISDTFASLSASDFEKG
                     HVIIKKGKKVFHKAVLKK"
     misc_binding    complement(21197..21443)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(21528..22193)
                     /gene="spoIIR"
                     /locus_tag="PLD98_00130"
     CDS             complement(21528..22193)
                     /gene="spoIIR"
                     /locus_tag="PLD98_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HQA04700.1"
                     /translation="MKNKVLSAFNKSASKLSKNGFVQAAVIVLILISCIISTAAFSTN
                     CKEVRDNVLRLHILANSDSEEDQALKLKIRDKLLTEGADIFAGAEDADSAAEKIAAAA
                     ERLETLAEDTAREMGYNYDVKVELTEEYFDTRSYEDKTLPAGKYRAVKVIIGSGEGKN
                     WWCVMFPPLCLPAAEEHDDKDVKIDAILTSGGARLIEKNPKFEVRFKIVEIYEKIKSY
                     FND"
     gene            22497..23849
                     /gene="ypeB"
                     /locus_tag="PLD98_00135"
     CDS             22497..23849
                     /gene="ypeB"
                     /locus_tag="PLD98_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HQA04701.1"
                     /translation="MKHGLSRRGKIRIISFLSILVLVLSIWGGIETYKRRTYQQLVKI
                     SQERALAELGNYVTNISVALQKGRYANTPPMIANLASSLWREATGAKTCLSQLESGGT
                     QLYNTYKFLSQVGEFTMALNRKVAADEHITDEERKQLDELLKYAQSLADQINYMQSMY
                     ENDALTFDAKKQGLLLSDAADTQKLSYVEGIANAEQTMTDYPTLLYDGPFSDNIDQKE
                     SKLLKESDPVSREEARKIAASFLGADPSQVADAGDEEGKMAAYLFSYESKVINITKKG
                     GFISYMLCSDFAGEEKIDQRQAVDIAKKYLEKHGYKNMKESYFSTNDGICTINFAYME
                     DDVVCYPDLIKVNVALDTGNVCGIDARGYIMNHHERKIPENIVSSDKAKQSLSKNLTV
                     QDVQLAFIPKDNGDEELTYEFLCKSKDDQDVLVYINVDSLEESEILILLYTDGGMLTK
                     "
     gene            23944..24195
                     /locus_tag="PLD98_00140"
     CDS             23944..24195
                     /locus_tag="PLD98_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04702.1"
                     /translation="MKKTIVLLCALLIAFAVLFTACNKAGDKDKMTMDNQTTTQPAGN
                     ITTANETTSKGAVESKAEEIGTTIKDNLESIGEGVSTNA"
     gene            complement(24320..26350)
                     /locus_tag="PLD98_00145"
     CDS             complement(24320..26350)
                     /locus_tag="PLD98_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HQA04703.1"
                     /translation="MKRIHKITTGVIALILAFGMIFSTCASAAYIEDPGMEGIAELFN
                     SSVNTIKEKRPAYNVIKRQGIAGTDNNDIAYHFQKVRVYYDLVGKVLDSGIFDNFLGG
                     ILGEFFFNLLGKELKDVLGSDNASQIDDLVNKITGPFILNNSLTDNAKFVIKGIPAEN
                     TVSVTGKPYVSALTKEDIYQYEFYPEFNGGYTIKIKFADEINPTENSPMGRVFDLIDT
                     QKLANVVKTVVSDFDPKDAKFKYQNAYAECTVNGNGQLTYYKTHYEINLGLKDLVAEA
                     FVFLDLSNAEIFDKSLYIVEQEYKDFNWNPILVGDVNSDDKINAQDARQVLRVYAMLD
                     KSNEGIYLSGDVNGDGKTDAQDARLILRHAAKLQLIKEKYVPTVPQQNSVEENGSEAK
                     VSELFMPYGKVLGAYGKANESRGGYYSVGDADLNGKVQPADARILLRIVARLEEYHGS
                     YFDVINVNDKDTTINAADARLILRHCARLETLPQKTVFVLDSDRPACAVPRIPEGDDE
                     GEPKQEGNINAHLRAPLYAPFDNPNKKYEDLSDFEYEVIRLVNVERAKVNLPPLQVSF
                     ELSEIARQRSVDMANGWPFRHYFDNKGNYISEGIALKMMKDRNLKYAWAGENIAAGPD
                     TPQDVVNAWMNSHSHRANILRAEFTAIGVGFTNDTTTIYDTYWTQIFASPQK"
     gene            26560..27159
                     /locus_tag="PLD98_00150"
     CDS             26560..27159
                     /locus_tag="PLD98_00150"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HQA04704.1"
                     /translation="MKLEYRGIIKSVETLPKTDLPENAVKFKEPETLAAVNKAALFYM
                     VPLAAFICLCVYLGVKFGGKTNPLDTPLYPGAILALLSVLPHELLHAVCFGKDAVVGL
                     YFAPKELMAFVFCSKPISKARFIFLSLCPNLVLGWLPFMLWLILPDLGKTGSNLLIFS
                     VYSILMGAGDFMNAVNAIRQMPKGSYQQLSGMHSYWFMP"
     gene            27226..28377
                     /locus_tag="PLD98_00155"
     CDS             27226..28377
                     /locus_tag="PLD98_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA04705.1"
                     /translation="MADNILDRFDKVFTEEKEVDASKKIKVGIIGCGWIAEAHVKSYL
                     KCPDVEIVAGADLIPGKAEKFFKDQGIENVRCYNSHKELIDNEELDAVSVCTYNATHA
                     ECTIYALEHGVHVLLEKPMCVTLEEAVEIRRAEKKSGKVLSIGFQPRFDENMKMVKRI
                     VQSGELGEVYYIQTGGGRRRGIPTPYGTTFIEKETAGIGAIGDIGCYSLDMVLNAVGY
                     PKPLTVTGYISDFFGKNPKYMAKPEYADVFGVDDFAAGFIRLEGGIILDFRISWAMHL
                     DTPGDTIILGKDAGLRIPSTDCWNGSIGGPLKVYHDVAGECVVTELPMAKNKGDNFDK
                     KIRSFVDAVKYNLPAPVPSEQIIINQAIIDGIVKSAQLGREIAIEIPEI"
     gene            28906..29274
                     /locus_tag="PLD98_00160"
     CDS             28906..29274
                     /locus_tag="PLD98_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04706.1"
                     /translation="MRSTRSKNEYRRLLCLLLRIEHKMPLDQVSEIVGFNKCYISKLV
                     SQYLKHGLDAISDKPMGGNRRNMTYEEEEEFLRPFFERRSKGEIVTAAEILKKYREVT
                     GTNAASSTIYRLLDRHNWKR"
     gene            29489..31756
                     /locus_tag="PLD98_00165"
     CDS             29489..31756
                     /locus_tag="PLD98_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA04707.1"
                     /translation="MKYEELLKRLSEDFGDWWKKGYEIPEMTNNLYPYEKLFSPIRVN
                     SITIKNRLVMGPMGNISMCEETGRPNDKMLKYFEERARGGVGLITTGLIPVSFGIDKS
                     LIELGELSYFPRIDRSRTVFAGWRDLAAMCHSHGSKIFIQLTPGLGRVGNPQCLVNQK
                     KFPRSASFNPNWYMKEVPCLRLSDRSLRAIIKRIGQGSADAKAANIDGVYLHGHEGYL
                     LEQCTNPAFNRRKFGRYANYQNFGLDMIREIRRRVGDDYPIMYRIDLSLMLNATYGDK
                     MNTVSPLKKFKNERTVAQTLEYMKNLVKAGVDMFDVDLGCYDNWWLPHPPGSMPSGCY
                     LDIAKLVKDYFKENNILSNKGLEVPVVAVGKLGYPDLAEKALREEKCDMIMLARPLLA
                     DPEWCNKAFAGRTKDIIPCIGCQEGCINEFVEGGHPQCAVNPRTAFEDEFSREIPKAE
                     IQKNVAVVGAGPAGITAAGVLLERGHKVTLFEKESVVGGNIVPGSYPKIKYEIKNYLA
                     YLTHCVEKYQENPNFTLKLNTAPTASELKEAGFDVIIIACGGTQTRPPVKGIDKPNVY
                     SAIDILMKPELIKDAKKVVVIGGGVVGAETAYFLKYEHNKDDVKVVEMEKYIMNHTCT
                     ANRGHIIYYLEKAGVELLNCTTLTEVTDKGVKVRQNTHKNVPNPYVTWSPILPENVEN
                     PMDRFSKIKDEHKERFIECDAVVVATGVKSADSLFYECQKVFAAPEIYNVGDSFKTAK
                     IFEATRSAYRKARSI"
     gene            31786..33102
                     /locus_tag="PLD98_00170"
     CDS             31786..33102
                     /locus_tag="PLD98_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HQA04708.1"
                     /translation="MSTVETRAGEYKMELTQEQLDILEGKKGETMAKVIKTVVMFGEI
                     FGAKRLVPITHNSGHLVTSFGIGILAPLYRTMDELINAGIKVKGDFTVDPRPLDYKNV
                     KCTMLEKLLFNKILYSKQEQYESQLKKVGLKDEDGFTCTCYLPEVGNTPKEGDILAWA
                     ESSAVVYANSVLGARCNRNSGMLDLFSSIVGYVPEFGLLTDEGRKASWKVYVNTTKKP
                     EAQVLGSAIGMKVMEDVPYVYGLDKFLGTELNDENKSYLKDFGAATASNGAVGLYHID
                     NLTPEAKRCGEELIKPDAKEYIIDDAELERIKNSYPIMWKDPNKKADKCFIGCPHLTL
                     QQLKDWTDKIEKGLKETGRKKVALTTVFTAAPGVVKEFQKSEHYPRLMAAGAKLSSIC
                     PLMYTNNPLTKKYNIATNSNKLRTYSAARFYEDDEMLAIIVGKEGH"
     gene            33102..33542
                     /locus_tag="PLD98_00175"
     CDS             33102..33542
                     /locus_tag="PLD98_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HQA04709.1"
                     /translation="MKSFKGRVIAGGNFKGEAVVSRQGLNTLATFQKSALTRSKEVIG
                     SDQNNPDVFGKKLTGKVLCLPQTIGSTTGGLVIQTVCSLKANPAAFLFSEHIDSLAAS
                     GIVLAKVWENSDVIAIDLLGQEFLDTVKTGDTVEIKEDGTVTIL"
     gene            33659..34339
                     /locus_tag="PLD98_00180"
     CDS             33659..34339
                     /locus_tag="PLD98_00180"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HQA04710.1"
                     /translation="MIKEDILKNMYLAGSAYSQTQADVFGGGVRVIDDAESGIQCFLR
                     RDGGTLYITFRGSDSPKDWQTNLYFRRLVIPYGNTKSKIRMHAGFIYAYNNDRVRPQL
                     HSAAKNTDKIVISGHSLGAALSIVCAVDIQYNFPEKDIEVYAYGGPRVGNLAFIRSYN
                     KRVFKTFRVENGNDIVTKLPPAIFGYRHAGIKVHVGQMRLPLVFSFNQHRPENYAKNI
                     IRDFYNTS"
     gene            34423..35499
                     /locus_tag="PLD98_00185"
     CDS             34423..35499
                     /locus_tag="PLD98_00185"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HQA04711.1"
                     /translation="MKHTYISKSALRVISLILLLSIAFIPVLFTGCSKKDEDLTTLPE
                     TEPQADPEKINPFTGLKYEKSKVSSRPVFVSINNAPAARPQWGLTSPDMIMEFEVEGG
                     ATRMVWVYARAEDVPEKIGSVRSARHYFVEMAEGFDAIFIHWGGSPYAYDRMKELKTD
                     HIDGMSYSTKYFARDESRKVGLEHTGYTTGSNIISAIKDLKVRTEIKDTYKNPFNFAP
                     ENSPRELSGQSCSEINAVFSSIVKYNFKYKAADNRYYSYLNQNKTVDDKGQQVAVENV
                     FVLFADVESMNTAKGHVTYDLSVGEGYYAYGGKAERIKWVKGSDRDTLKLYDLNGEEL
                     IINPGKSWMGFVKTANEKQSTIVA"
     gene            complement(35535..35765)
                     /locus_tag="PLD98_00190"
     CDS             complement(35535..35765)
                     /locus_tag="PLD98_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04712.1"
                     /translation="MTQKRKEELKEALPILKDAYDIIEAVFSDEESAYIDTPEEFYDT
                     DEYEEMENAVTQLEEILYQIEEAMESLKELLE"
     gene            complement(35924..36424)
                     /locus_tag="PLD98_00195"
     CDS             complement(35924..36424)
                     /locus_tag="PLD98_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04713.1"
                     /translation="MGLFVVSVIGFIVCGIILLIKAIRKKPKKVIGVLTGVFFVVALA
                     TMIWAMNTPAVDPTQMIGEDPRPKAVEADFVKINAGEWEGKFVKAVGEVSLIELKTDQ
                     IIIFDLTTQEGDGYGVYSVEAAFNVGEIERDIAEGDRVVVYGTVEKPNDLMIPVIGAS
                     IIEKGE"
     gene            complement(36743..38548)
                     /locus_tag="PLD98_00200"
     CDS             complement(36743..38548)
                     /locus_tag="PLD98_00200"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HQA04714.1"
                     /translation="MSYPKIGIRPIIDGRWGGVRESLEDATMAMAKAAKEFIESNVYY
                     SDGTPVQCVISPCTIGGGAEAAKCAEYFATQNVVATLSVTRCWCYGSETMDLDPLTIK
                     AVWGFNGTERPGAVYLAAVMAAHAQRGMPAFAIYGKDVQDATDTSIPEDVAEKLLRFA
                     RCAVAVGQMRGKSYAGIGSVSMGIAGSAMDPMFFQNYLGIRPEWIDMTEIKRRIDLGI
                     YDHEEFERALKWVKENCPEGFDKNPKPHTREQKDKEWEFVVKMTLIFRDIMLGNPKLA
                     NIVPVGEEAKMPGALDSGWHEEALGKNAILSGFQGQRHWNDYLPNGDFSEAILNSSFD
                     WNGKKEPLIFATENDGLNGVAMLFGKLVTNTASLFADVRTYWSPEAVERVTGKKLTGR
                     AANGFIHLINSGSAALDATGMCKDENGNPVIKKWWEVTDEDIKNMLSVTDWCPADTGY
                     FRGGGYSSHFRTRAEMPMTMIRVNLIDGLGPVLQIAEGYTVVLPDDVHEALDKRTDPT
                     WPTTWFVPNLTGKGAFRDVYSVMANWGANHGALSYGHIGADLITLASMLRIPVCMHNV
                     PEEKIFRPHAWSAFGTADLESADYRACQTYGPLYK"
     gene            complement(38592..40064)
                     /locus_tag="PLD98_00205"
     CDS             complement(38592..40064)
                     /locus_tag="PLD98_00205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HQA04715.1"
                     /translation="MSEKRVLAFDFGASSGRAILGTFDGERISLNEIHRFLNEPVKVG
                     DTLYWDILRLFHEIKQGILKAKHLGGFDSIGIDTWGVDFGLLGCDGALLENPVHYRDE
                     RNNGMIEEADKIISREELYSLTGIQFIQFNTLYQLLSLKLKRPHILERAKKLLFMPDL
                     MSYMLTGEIFSEYSIATTSQLVDLKTGEWSYSLIDRMGFPREIFAPIIKTGTEIGRLR
                     ADIAEELGIDRVRVIAVAGHDTQSAVTAVPSLNKDFAFISSGTWSLLGTETDSPILTK
                     EAMELNITNEGGYNRSIAFLKNICGLWLIQESRRQWIREGEEHSYAELEKFALQSEPF
                     KCFIDPDAKDFLSPGDLPNRIREFCRRTGQYVPESKGEIVRCIYESLAMKYKYTLLGI
                     EKCTKKVYDKIHIVGGGVKDGLLSSMTADSCARPVHAGPIEAAVLGNIAVQLLADGSI
                     ESIEKAREIISRCEDIKVYEPKNPAAFDAAYDRFCGIVGK"
     gene            complement(40081..40506)
                     /gene="fucU"
                     /locus_tag="PLD98_00210"
     CDS             complement(40081..40506)
                     /gene="fucU"
                     /locus_tag="PLD98_00210"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HQA04716.1"
                     /translation="MLKGIPDIISPELLKILHEMGHGDELVIGDGNFPAASNAKRLVR
                     CDGHTVPPLLDAILQLFPLDTAVESPVILMAPMPGDTVDPNVKKTYAEIVAKSGDFKI
                     SEIDRFAFYERARQAYAIVATSETALYANVIIKKGVVVR"
     gene            complement(40562..42064)
                     /locus_tag="PLD98_00215"
     CDS             complement(40562..42064)
                     /locus_tag="PLD98_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA04717.1"
                     /translation="MTSAEENARLNTAEIISAPSAEPEKKMREYLSPKAMAAYIISGI
                     GDKNWESFSSQNTYFFNTTFLRASPTTLALVDSLCGIADALDNAISGPIIDRTRTRWG
                     RVRPYLVATLPLWLLSAAMPWLLPTNLSQTALFFILLILQYLGSIANSFYGPAYAAIL
                     YNLTPNVDERDRLIAADTYVDLFGVWIPSLFPFFIDYLPGNIPTRYIYIGGAMFFIMI
                     VAVFRIYGFLTLRERIPLASRQTMQQYSVFDSVKMVANCRPVWVLLIKGFFGVGKGIG
                     VNVESYFWLNCTGKLSNQFFAGLFTGIPSYFVLPFATKLTEKMGLRNLGTFCFTFSGM
                     SYLILYLVGYKPTSSSFLNMAWVVLGLTFAGAFNSVQRYCNTALQGDMYDYVEWKTGF
                     RNEGMLSAAMGYMGLATNTLGKLITGAVIKAIKYKPLINAAGKVVPQTDPRMLRAIWT
                     VFSLVPAISRFLEAGALRFFNVHGSFKERMLQELAGMRAERIAALNNGDE"
     gene            complement(42252..42719)
                     /locus_tag="PLD98_00220"
     CDS             complement(42252..42719)
                     /locus_tag="PLD98_00220"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQA04718.1"
                     /translation="MKSLWFCDTAIGKIGIAEENGELTDILYPGENPDFDYTYKETPL
                     IKEAHKQLSEYFNGERKKFDLPLRIDGPPFQTKVWNALMEIPYGETRSYKDIAVRVGN
                     PSGSRAVGCANGKNRLPIIIPCHRVIAADGTPGGYGGGLNMKKFLLNLEIKHR"
     gene            complement(42747..44045)
                     /gene="lysA"
                     /locus_tag="PLD98_00225"
     CDS             complement(42747..44045)
                     /gene="lysA"
                     /locus_tag="PLD98_00225"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQA04719.1"
                     /translation="MFVSECLNVNEKGHLTIGGCDTVELANQFGTPLIVYNEETIRKN
                     CREFVSSINEHYEGKGLVYYASKAFSCLEMCRICKSEGLGLDVVSGGELYTALKADFP
                     PEKICFHGNNKTDSEICEALDAGVGRIIVDNIEELRKIGRAAAEKNKTAKVLLRIKPG
                     IDAHTHEFIKTGQIDSKFGFALETGEAMEAVKLALAEENIELMGLHCHIGSQIFDIDP
                     FILAAEVMIAFIAEIKKETGTELTELNLGGGFGIKYTGDDNPESFSEYMKKVALSLKA
                     TAEKYGIKTPFVMIEPGRSIVGAEAVTLYTVGSVKEIPNIRNYVSIDGGMTDNPRYIL
                     YGSKYDIHCANKMNEPRDYMATIAGRCCESGDLIQKDAMIQKTQEGDIIAVLSTGAYN
                     YSMASNYNRVPRPAVVMITDGKPRLIIKRESYDDLIANDI"
     gene            complement(44073..44666)
                     /gene="yihA"
                     /locus_tag="PLD98_00230"
     CDS             complement(44073..44666)
                     /gene="yihA"
                     /locus_tag="PLD98_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQA04720.1"
                     /translation="MNFNIVEFEASFGLSSQLPKSNIPEIVFAGRSNVGKSSMINKLF
                     NRKQLAKVSSVPGKTITINFYRLGGVRFVDLPGYGYAKRSAAEIRRFGELMEGYFNSG
                     RNIKLIVQLIDMRHSPTSDDIDMINFMIAKDLPFVIVLTKSDKLKPTERQKRLEALNE
                     ELAFLGDREVEVIEFSAQTGVGLKELRSYIDDVLEQK"
     gene            complement(44774..47185)
                     /gene="lon"
                     /locus_tag="PLD98_00235"
     CDS             complement(44774..47185)
                     /gene="lon"
                     /locus_tag="PLD98_00235"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQA04721.1"
                     /translation="MMEGAKSLQMPLVALRGLVIFPGMTLHFDVGRKASTAALRAALE
                     GNQDLFLVTQRDINEEEPDLNGLYPMGVISEIRQVFRIHEGSTYRVIVEGKHRASIDS
                     LVQEKPYMIASIKPKYDPPIKSVDKELALALVRRAKNLFEEYSELSPKIAPDVIASVE
                     QEERPGILSDLIAAAIPLPMQDKQNLLSQKNPLRRIEQLCVILTKEIDILRLEEDIQD
                     RVQEQIDLNQREYYLREQLKVISEELQEGDSPASDADNYIAKIKESKMPEESKKKLLE
                     EAGRLRRMSPASPDSAVIRTYLDRCLALPWGVYSKDKNSLASVRRVLEKEHYGLEKVK
                     ERITEMLAARFLSKELKGQIICLAGPPGVGKTSIAKSIATALGRKYARLSLGGVRDEA
                     EIRGHRRTYVGAIPGRIITAITQAGTSNPLILLDEIDKLSGDFRGDPSSALLEVLDSE
                     QNFAFVDHYLEIPFDLSQVLFITTANDTSAIPGPLRDRMEIIELSSYTAEEKFHIAKR
                     HLVPKQIKQHGLSRKQIRISDKALRLIIEGYTREAGVRRLEQLISKLCRKEAVRLADG
                     IKEPLVITDKNLSEFLGPVKYKEDPSLYADQTGIANGLAWTSVGGEMLQIEVAIMEGT
                     GKIELTGQLGDVMKESARTAVSYLRSKASQLGIDSEFYKNKDIHIHVPEGAVPKDGPS
                     AGVTIATALYSALTGKKVNGKVAMTGEITLRGRVLPIGGLKEKTMAAYRTGMTTVLIP
                     YGNLSDLKEIDKAVEKALKFVPVRTLDEVFDCAILNEKPESAKGEIHILTPPKVSDGA
                     QVWTT"
     gene            complement(47565..47909)
                     /locus_tag="PLD98_00240"
     CDS             complement(47565..47909)
                     /locus_tag="PLD98_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04722.1"
                     /translation="MLESLVAGLTLIFALIGLLAIIYAGTLKLLSPKKNKEYYLVLPL
                     DNEEESITKICAAIEKRNLLGETHCKIIAVDMGLSFETFKYIERMCLTNKNMELINKA
                     DAPTFFEELLHG"
     gene            complement(47952..48287)
                     /gene="rnpB"
                     /locus_tag="PLD98_00245"
     ncRNA           complement(47952..48287)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLD98_00245"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(48385..49173)
                     /locus_tag="PLD98_00250"
     CDS             complement(48385..49173)
                     /locus_tag="PLD98_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQA04723.1"
                     /translation="MDYRFNSISLTRFASTICALAGIEKPEKADEPVESILKLFNDAP
                     FDRIVIHNPDAVAMWLYQQNFELFEKVLRHAPVCLPFKTVFPSVTPVCFGTMYTGALP
                     EVHGIQFYTKPVIKIDSVYDAFIGAGKKAAIVAVRESSMSKIFLERDMDYFIEEDDEK
                     VKETAIRLIKENKYNLLSVYTQEYDDVMHARGVLSPEAINALKNQIRIFDELANAIKE
                     TERDGKTLITFSTDHGVHNEPNGRGSHGTDEPYDLNIAHFFGVL"
     gene            49356..51563
                     /locus_tag="PLD98_00255"
     CDS             49356..51563
                     /locus_tag="PLD98_00255"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HQA04724.1"
                     /translation="MDKILSASFLKAPKYKEGAAFVFSKEFESDKPIKSATLQISSLG
                     VYVAYLNGQRVGDFILAPGWTVYEKRLQYQTYDVTDLIKDGKNIITAEVGRGWRFHKR
                     PQHATKHIKADEPALIASLCLTYNDGSEAIINTDSTWKSSESKTVYNDMYGGETYDDN
                     FVSEEKSAVAFPHPKEILIPQEGEKVAEKERLKPQRLITTPECDTVIDFGQNLTGYVE
                     FKAKGNKNDELVITHFEVLDKNGNVYTENLRKAKQQVRYICSGEEKLYKPSFTFYGFR
                     FIKLEGFKDVNPDDFTAIAVYSDMKRIGHFESSDPLLNRLYENIIWGQRGNFLDVPTD
                     CPQRDERLGWTGDAQVFIRVASMNYDVSTFFRKWLNDMAAEQGEDGRITHVIPATFDG
                     GGSAAWGDAATICPWQLYLSYGNKEDLEHNFPMMKKWVDYIRKSAAHKSLIQCFKGSR
                     NPYLWNTGKHFGDWLALDIEGDDEDARGATDHDYIATAFFAYSTELLIKAGEVLGKDM
                     SEYKALHENIVKAYREEYVNPDGTIKINTQTACVLALKFGLTPDREATANQLVSLIRK
                     YGHLTTGFVGTPYLLHVLTDIGEVELAYDLLLRKEYPSWLYPVTQGATTMWERWNGQR
                     PDGSFATPGMNSFNHYAYGAVGDWLYSTAAGITMRDGTVAYEDLIFKPQTDKRLTFVK
                     ASIETARGTVASEWRRETDKTVYTFTVPAGSKAEAVLEGKTYKLNEGVNTITL"
     gene            52015..53307
                     /locus_tag="PLD98_00260"
     CDS             52015..53307
                     /locus_tag="PLD98_00260"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQA04725.1"
                     /translation="MEKFVINGGKRLCGEVEISGAKNAAVAIISATVLADGVCRLENI
                     PNVRDVRILMQILDSMGAKVRNINNNTWEIDSSTINTTVVSNEMTRHLRASYYFLGAL
                     LGRYKQAKVGMPGGCNFGVRPIDQHIKGFEALGATVTTGNAMVEVKARELIGNSVYFD
                     MVSVGATVNVMLAAVKAKGLTVIENAAKEPHIVDLANFLNLMGADVRGAGTDIIKIRG
                     VEKLRGCQYSIIPDQIEAGTYMVAAAATRGNVFIKNVIPKHLDSISAKLIECGAEIEE
                     FDDCIRVSSNGRLNRCHVKTMPHPGFPTDMQPQMAVLLSVAEGTSIISESVWDNRFRY
                     VDQLIRMGAVIQVEGKAAIIEGVPFLKGAPIRADDLRAGAAMVIAGLIAQGQTTIEDI
                     HHIDRGYENVLGKLQALGADIKRVEFNTPVTKPVSIVG"
     gene            53371..54162
                     /locus_tag="PLD98_00265"
     CDS             53371..54162
                     /locus_tag="PLD98_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQA04726.1"
                     /translation="MARFTTLFSSSSGNSTYLGTSSGGILVDAGKNAKQLEAALSAIN
                     VSPTEIKGIFITHEHTDHISAVRVFASRYGIDIYASKGTLAELRMQGHLNGKFNAIPI
                     DEKGAVVAGIHIAPFRTSHDSRESTGYTAVFEDGRRIGIATDTGVMTQEILTALSGCD
                     AVLLESNHDVWMLENGSYPYYLKRRIRSNLGHLSNDDCASALCRLIQTGTTRIILGHL
                     SKENNRAEIARQTSVSALSTIGAVENRDYILRVASHDRPDKMLIF"
     gene            54199..54681
                     /gene="rlmH"
                     /locus_tag="PLD98_00270"
     CDS             54199..54681
                     /gene="rlmH"
                     /locus_tag="PLD98_00270"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQA04727.1"
                     /translation="MIKVNIICVGRLKDAFFEEAAKEYIKMLGSFCQIKITEINAANL
                     PQEPSRAEIEKALMAEAKEILRKIPERSKTIALCIEGKQLSSEEFADLLEGCKSSGAG
                     EISFIIGGSYGLADDIKKRADFRLSFSKMTFPHRLFRVMLLEQIYRAFSISAGGKYHK
                     "
     gene            54922..57564
                     /gene="ppdK"
                     /locus_tag="PLD98_00275"
     CDS             54922..57564
                     /gene="ppdK"
                     /locus_tag="PLD98_00275"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQA04728.1"
                     /translation="MSHKYVYLFSEGDGSMRELLGGKGANLAEMTKLGMPVPQGFTVT
                     TEACTRYYEDGKVIAQEIQDQIYEYLAKIEEINGKKFGDPEKPLLVSVRSGARASMPG
                     MMDTILNLGINDEVAEGLIKGNPDPSFARFVYDSYRRFIQMFSDVVMEVPKHNFEVII
                     DELKAKKGVKDDIELDVEDMKELVRLFKAYYKEQKGEDFPSDPRAQLMAAVQAVFRSW
                     DNPRANVYRRMHDIPYSWGTAVNVQPMVFGNLNSNSGTGVAFSRDPATGENVLFGEYL
                     INAQGEDVVAGIRTPSHISKLKEEMPEVYEQFVTIAKKLENHYKDMQDMEFTIENGKL
                     YMLQTRNGKRTATAALKIAVDLVDEGLIDEETAVLRVDPKQLDALLHPQFDAAALKAA
                     KVIGTGLAASPGAATGKVVFTADRAKELAEKGEKVILVRLETSPEDIEGMAAAQGILT
                     VRGGMTSHAAVVARGMGTCCVSGCGEIKMHAEEGYFELGGKKYYEGDYISLDGSTGKI
                     YDGEIKTVEASISGDFGRFMAWADKYRTLKVRTNADTPTDAATAVHFGAEGIGLCRTE
                     HMFFEGDRIKAIREMIVSKTAEARAKALEKILPLQQKDFEEMYRVLEGRPMTIRFLDP
                     PLHEFLPQKENEIKELADDLGISVDELKAVIDSLHEANPMMGHRGCRLAVTYPEIARM
                     QTTAVISAAINVNKEHPEYNIVPEIMIPLVGEVKELKFVKDVVVETADKLIAEAGVKL
                     EYKVGTMIEIPRAALTADEIAKEAEFFSFGTNDLTQMTFGFSRDDAGKFLDAYYDTKI
                     YEFDPFAKLDQTGVGKLVEMAVKLGKATRPDIKLGICGEHGGDPSSVEFCHNVGLDYV
                     SCSPYRVPLARLAAAQAAIKAKKA"
     gene            complement(57611..58666)
                     /locus_tag="PLD98_00280"
     CDS             complement(57611..58666)
                     /locus_tag="PLD98_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04729.1"
                     /translation="MRITTDFTGGNIKVLSVEGDYVKVECDLRDTTTDWFYWAFCVKG
                     ASGRTITFDFGRDRLGYFGPAVSKDLKTWNWAGEETLGDNRFSFTYTFDDDSPTYFAH
                     DMIYSPERFEEFLKKHNLNALTFAVTDKGNEVKILEFGGGDKTIVLTSRHHCCESTGT
                     YVLEGVLEHLLKNLPDGYRVAAVPFMDIDGVIFGDQGKNRHPHDHNRDYIENPVYPSV
                     RELMAYAKEHNVKFQFDFHSPYHIGGGDDKVFLVRNLLNTKGQDEFASLFHEESANLS
                     FRYFVGSDIMPNTSWNTLTNPTSTKFFSSLPTTELSFTLETAYFGTEENKVSQEGLIE
                     LGKAFARALIRYIENHR"
     gene            complement(58718..60070)
                     /locus_tag="PLD98_00285"
     CDS             complement(58718..60070)
                     /locus_tag="PLD98_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA04730.1"
                     /translation="MKDARISGRTWFNIIVFGFMGQVAWAVENIYFNTFLFNYIGGTT
                     KDISTMVALTAATAVIATFLMGNLSDRLDRRKVFLSLGYILWGFSVMVFAFISRDNVA
                     KLFGLADEVKILAASVSVVIIMDCVMTFIGSTSNDAAFNAWITDITVPENRGKAEGIL
                     ASFPILATLLVTVAFGAGVAAAGYPACFLGLGIGVSLCGLIGAFTLKDSREGKPRRQG
                     RYITDLIYGFRPSVIKENALLYLALLAVSFFNIAVQIFLPYLFIYLQHYMGFDFENLN
                     ITPKVIGIAVVAIVLFIAVVYCLGLMVDKFGKDKFIYPSIALFIIGLIATSQAKKLGA
                     FAATVVMVFAGYGLLMIILNASVRDFTPEDKTGLFQGVRMIFMVLLPMVIGPPLGNAV
                     IERFAPLHQLGTYYNDFGEAVYVPVPEIFLAAAIFSVLIVIPIFFLRKKAKAQNKKGY
                     "
     gene            complement(60175..>60682)
                     /locus_tag="PLD98_00290"
     CDS             complement(60175..>60682)
                     /locus_tag="PLD98_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQA04731.1"
                     /translation="LAVIGHSRLGKTALVAAAFDERFRFVHSNNSGCSGAAIIRGKTG
                     EQIDDICRNFPFWFCENYKTYRKNESALPFDQHLLLALSAPRFVSVGSAAEDLWADPA
                     SEFLACAAASPVYSLYGKEGLIAPDTFPPEEGAYQVGSIGYYLRPGSHFLSRYDWNKF
                     LDFIESKV"
BASE COUNT        15424 a        14691 c        14625 g        15942 t
ORIGIN      
        1 acaaaaaaag tcttggttcc atgtaaagcg tctgtaatcg aggtggtttc ctcctgctcc
       61 tcggtggtgt tcgtgatgtc cccgcctccg accagattcg acaaatcgta gtcaagggag
      121 tgaaggagca tcaggaagga tacggagccg aaaagcagaa caaaacagac aaagctgatt
      181 acaaacgcct tgacccgctt tgcctttaag ttcttttcgg ttgtgaattt cagcttcctt
      241 cgccttctca agatacgcac cgccatcact ttgacttctt aaaactatta taccccaagc
      301 aaaaagggaa aaacaatagt cacaggctaa ataaagattt atttattcac ctgaggaaat
      361 atgcaaaaca tacaatcgct gtatgcaaaa ccgccataag cggcaggaga atcataaact
      421 ttttgtgcct tgtcttgtgt cttataacta tcattacaag gtacatggga agagcgccgc
      481 ctaaaagagc aatccataaa aggctgcttt cggggattct tcttttcctt ccgatagcgg
      541 ctattttatc atacagcgtt aaaatggcgg aaattgtccc tattaaggcg atgtaataaa
      601 aaaatggctt catattctct cctctaaaaa agacgcacct ttttcaaggt gcgtctgatg
      661 cgctttcatt taagatttat gccgctgcat caatggttct ggggaatgct gactcagcaa
      721 agccgagtgc cttagcgacg cgaacgggaa tttccctgtt ctttattgcc tgaccgggct
      781 taatgaagtc gttgcatccg taaactctga gcgggacatt tgcgccggag tgcgagccgg
      841 aagtgaactc atatgcgccg ttcttgtact taatagcgcc tgtctcgtgg tcggcggtta
      901 caacaataag ggtgttgcct tctgccatag caaactcggt tgctatctgg attgccttgt
      961 caaactcaag gagagcttcc ttcatttcct cagccttgtt gctgtgggag tgcttgtcga
     1021 tgtgagcgcc ttcaaccatg aggaagaagc cgttctcgtt cttaagaagg tcgatagcag
     1081 cctctgtgag ctgtgaaagt gtgggatagc tgctgccgcc tgccagtctc caaagctcgt
     1141 ttgtgaactg accgaagctc ttatcgccgt cttcgagcgc gagaatgtct tccatggatt
     1201 caacatactt atagcccgtt tcggctacga tttcgggagt tacaagacct gttgaaaggc
     1261 cccaaataag gtcaattccg cttgcaagct gctgcttggt aatatcttct gtgtactttc
     1321 tctggaatac atgagcggag aagcctgcgg gggttgcgcc tgctgttgag tcagaggtaa
     1381 ctatacctgt cttcattcct ctttccatgc agagctctgt aaggttcatg gggtagcttg
     1441 cataggcaaa ggggtcggta atatatacac cgactctgcc gttagctgtg cggataccgc
     1501 atgcaagtgc tgttgcgcct gctgcgctgt cggttacgcc gcctgtgtag ttggatgttt
     1561 ttgagaagcc ctgaaccggc agtgtatcca tgaacagttc ttcgcctgtt tctgcctttg
     1621 tcttttcaag gtggttcggg cccatgccgt caccgatgaa gaaaatgaca ttcttaattt
     1681 cggtttcggt ggtctcggta cctgttccgc caatgccggc aaagagcagg ctgagaacgg
     1741 tgagcattgc gaaaaacttc tttagcaatg tgggaagcag attaatgtcc ataacgcatc
     1801 tccttgtatt tatattgcag cctgtggccg ctgttttccg tagtttgttt tttttgaaat
     1861 ccgcagccta attttctaaa taattatagc acaaattata aaaaaatcac tcgaaaaatt
     1921 cattatattc atataattta taacggtaaa atctgagctt tccctttttt tcggcgtctt
     1981 tgaactcgcc cttcattttt atgaccttac aggatgtgta aagctcaaaa tcaagctcgt
     2041 cgtagtgcag agcctcggtt acctcgggaa cggtgatttc cagcaccgca atgtcatcct
     2101 cggggaaaac tcggcagttg agagccttaa tctccacccc gcccgcaacg agcttttgaa
     2161 agcggtcaat ttgcttttta tcttttttcc tctttttaaa cagcattttg acctcccttt
     2221 tattacggct tttcagccgt gattatatag cagcccgtat tgtcggggga tactctgacg
     2281 gatttcatat tctgggggat tttcagactc tcgcccgact gccggctgat gatatagccg
     2341 tttgcctcgt agaatacgcc gacttttaaa gactctatgt attcctcaag acaaaggtcg
     2401 tttctataca tgatttccgc gtggggaacg cccacatacc ttaaatgcca cggctcgaac
     2461 ttaatgccgg tgatttcggt tttgttctcg ggatagcgga taatcagccc gtatttgtag
     2521 gcgttaaggt ttacataaac accctcgcgg gattttataa acgcctcgcc cgcgtaatta
     2581 ttgacataca catcaagggc aagacccgtt ctgtgctcgc tggcgtccgg agcggcggct
     2641 gtatccttac ttttcgacgc cgccagttcc tcctgctttt ctctggttct gtaagctgaa
     2701 gatatgtaga gctttttgcc gtacttttta tttatgtact ccgccagctc ctcatagggc
     2761 ttcattatag ccttgtgcat aaagacattt ttgtccttat actctcccac atactcgtcc
     2821 gcgccttcgt aaaccttatg ctcggagtta ataaggataa ggctgttatc gtagtggatg
     2881 ttcgggtctt tcttgatctc gctgagcgtg tattccttta aggtatagcg ggactcgtcc
     2941 ctttctttaa ggcgaacttc ggtaaatatc ctgtcgagat tcagccttaa ctgataatag
     3001 tattcgtgct ttctgtttga aatggcgtct ttgttcttat agacaaaaac gcccgtaaca
     3061 agagtcaagg cgacaagaca taaggcgaag atggcaagac gcagcctttt gtttttgccg
     3121 ccgtttttct tttttgagcc ttgcttcaag ccctcagctc cttattttta aggatttagc
     3181 atttttcgga caaaagcttt gtcagctcct ccataaaggt gttaatgtcc ttaaactggc
     3241 ggtagaccga cgcaaagcgc acataggcaa cctcgtcgat gtccttgagt ttgcacattg
     3301 caagctcgcc tatgcgtctt gaggttatct ctctgtcaag agagttgtgg atttcggcaa
     3361 cgatttcgtc cacagcctgc tcaagccttg agatggagac agggcgtttc tcgcaagctt
     3421 tcagcatgcc gtttaaaagc ttgtccctgt caaaaacctc tctggatttg tcctttttaa
     3481 tgacaacaat gggtacgctt tcaataatct cgtaggtggt aaaccttttg gcgcatttca
     3541 tgcactccct gcggcgcctt atgcgctcgc cctcgtctgt cgggcgggag tctataacct
     3601 tgctctcttc aaaggaacaa taaggacact tcatcagttt acacccctta tatccatttg
     3661 aaaaataata caatattttg ccacaatata caagtcggaa aggcaaattt cacgaatttt
     3721 atcgctttgc gcccgctctt gctataagac ttataccaag gtatatgctg atgcacaaaa
     3781 gtgttatatt atagccgctt tttacgagta ttatgaacga cagaataaaa aggggcataa
     3841 gaaaggcaag ccctaccttg tcggtgattc tccccgcatg agcctcggtt cttttttgac
     3901 aaagccaaaa atatagcgct ctgccgccgt ctagaggctc tatggggagg agatttacaa
     3961 cgccgagacc gagattcgcg taaatgggca ttaagagtct gttgtcgccg cccgcaaagc
     4021 aataaaaaat actgagaagg gaggctatga ggaaattcac aaagacgccc gcaagggaaa
     4081 ccaccgtctc ctgacggtag gataggattt tgccgctcat tttcatttta aagcccgctg
     4141 cccctattat tatttctgat ggcggggcgt cgtatgaaat cagacataaa agatggcccg
     4201 cttcgtgaag cagcgccgaa acgacagtta tcataaagct ttctcccgct cccgagccta
     4261 taagaaaggc tgccatcaga gcaaaaagaa agctgagtct aatctttatt ccttttattc
     4321 tcatacctat ttcaaggcgt acttggggtc tcttactatt ccgtctatct gaacctcaaa
     4381 atgcacatgg ttgcccgtgg agagacccgt gctgcccaca agagcgatgg cctctccctt
     4441 tttaaccttg tcgccctcgg atttcagcag ctcaaggcag tgggcgtaga gggtttttat
     4501 ccccccgccg tggtccacga ctatatagtt tcccctgccc tttgtggagc ctgtttcgca
     4561 tacggtgccg tcatatgcgg acttaatttc ggtgccctcc atagcgccga tgtcaatacc
     4621 cgtatgaaag ccccattcct ttgtgatggg gtgctctctg aatccgaaac ccgatgttat
     4681 gtagccgtca acggggtaaa tgagctttat tcccaaagta tagggcttga aaaccacgct
     4741 tacaatctcg ccgtcgactt ccttcatatc ttccccgccg cctatgggct catcgtcatc
     4801 cggggcagtt tccgcgccgg gctttgttac ccactcatcc tcggagatga tattgcccgc
     4861 ctcgtcggtt tcgtaagcgt cacggtaaac atccttttcg tcgacgactt cgtcgctttc
     4921 gtctgcttct gcggtctcct cctcagcggg cggttccttg cccgcaagac gggcgacagc
     4981 ggattttatg gagtttgcgt actcctctcc catatcctcc ttcataagac gggagtattc
     5041 gccgctcatg cccccgaaga tattcttatt gagcttaaaa gcgccgagaa agacaagcat
     5101 tattaccgcc gcgacgatta tttgaaccaa ggtaagtctt acaaaaaagt ccgtaccttt
     5161 attctttggt tttagctttc tgttttttaa cctgttcata aaccttatcc tttcataaat
     5221 ttcctcaaaa aattatatgc ggacgacttt gtgcctaaaa ccaaaagctt tccggcagga
     5281 cttgtcagac aaaaacctca agtttataat tgttcatagc aaattaagga gagtttgtta
     5341 tgagcattga aaaaaggctg tttggaaaaa ctcctcaggg cgaagaggtt tttgagtttg
     5401 tgcttgcaaa tgacaatgct gaggtttcgc taatcagcag gggcgcggct ttgcgcagac
     5461 ttctggtcgc cgataaagac ggcgaaaaaa gagatgtgct tttgggcttt gatgatttgg
     5521 aaggctatat cgagaggtcc gattatcagg gagtagccgt aggtcccttc gccaacagaa
     5581 ttgagggcgg aaggctcaaa ataggcggaa aggaaataat agtccccccc aatgaaaagg
     5641 gcaggaatct ccttcacagc ggcgggcttt actctaatgc cgtgtggagc ggtgaagttt
     5701 cgggcgacaa caaggtagtt ttcaaaaaga gatttaagga tatggaagga ggcttccccg
     5761 gaaataccga tgccgaaata agctatactc tggagagcga cggcaccgtt cttatggagt
     5821 ataaggcagt gtccgacagc gatatgtact tcaatctcac caaccacgct tatttcaatc
     5881 tggcaggcta tgacggcggg gatatactgt cccacaagct gaaaataaac gccgacagct
     5941 ttttgcccgt tgacgagaac agcatcccga cgggtgaatc aagaaaagtt gaaggcactc
     6001 catttgattt cagagaattc aaggagattg gcagagacat tgaggcggat tatgagcagc
     6061 taaaacttac cggcggatat gaccactgct tctgcctgaa tgatttcggc aagggctgca
     6121 gactcgccgc ctcggtaaag tgcgaaaaga gcggtctttg ccttgatgtg ttcacagacc
     6181 tccccggagt gcagctttat acgggcaatt tcctcagcgg cactgtcgga aagggcggaa
     6241 agccgatgaa ccaccgctcg ggcttctgcc ttgaaactca gtactatccc gacagcccca
     6301 ataaaccgca gttcccttcc tgctatgtaa aggcgggaga agtcttcttc acaaaaactt
     6361 catacagagt ttacaggtac taaacaaagc caaggggcga cccttggctt ttgcgttttt
     6421 ccgcagaaat tttgtctaat tttaaaatat gatattttct taacctatct tttaacaatt
     6481 gacacaaaaa aatggctgtg atattataat tttgtgtcat aagcgattaa tttttacaga
     6541 aaatagtgtt tttgcaactc atttgcacta tacattattg aaatttttaa tcggaggtgg
     6601 gtttagtggg tttagtttaa tttaagtctt taaggactta agcaaaactg ccaaaaccct
     6661 caaaacgatt ttgggaattg gaggaaagca aatgaaaatc gagatcaatt cgaatctgtg
     6721 caagggctgc gagttctgca tagcattctg cccgaaaaag gtcttgaaaa tgcaaagcga
     6781 aagaggacca aagggctatt ttatccctta tttgaccgac aatgacggat gcacttcctg
     6841 cggaatctgc gcaaccatgt gccccgacgg agcaattgag gtctttaagg aggtagaaac
     6901 tgatggctaa ggtgcttatg aaaggctgcg aagccgttgc cgaagctgca gtcagagcag
     6961 gctgccgttt ctttgcggga taccccatca caccgcaaaa cgagattccc gaatatcttt
     7021 cacgcagact tcccgaagtc ggcggcgtat ttatacaggg cgaaagcgaa gtagcatccg
     7081 taaacatggt ttacggcgca gcaacaatgg gcgtaagagc tatgacaagc tcatccggcc
     7141 ccggtatcag ccttaaggct gagggtattt cataccttgc aagctgcaag ctgcccgcgc
     7201 tcatagtcaa tgtaagccgc ggaggtcccg gactcggctc cattcagccc gctcagtcgg
     7261 actatctgca ggcgacaaag gcactcggac acggcggatt cagagtaatg gtatttgctc
     7321 cctacacact ccaggaggct gttgacctta catatcaggc attcgactat gccgagcgcg
     7381 acaggaaccc cgtattcctt cttatggacg gatgtctggg cgccatcatg gagcttgttg
     7441 agctgcccga aatgaaagag ccgtccgatg aaaacacaaa gaagtggaac ctcagaaaga
     7501 ggcccgatga aaaaaggccc gcaagagcat tgactccgat ttatcccgag ataatcctcg
     7561 agatggaaaa caagacccgc ggcgagcttt ataagaagtg ggagcgagag gatgtcaagg
     7621 tcgaggagtt catgattgac gacgccgagt atgtggtagt ggcatacggc attgcggcaa
     7681 gagtcgcgaa agaggcggtc ataactctca gagagcaggg ctacaaaatc ggcatgataa
     7741 ggcccattac cctttatccc ttccccaagc agtctttcca caagctgaac tacgacaagg
     7801 taaaggcaat aatcgatata gagctcacaa ttcccgctca gatgcgtgac gacatagccc
     7861 ttgaggttat gggcagagct cccatattcg agtacggccg ttcaggcgga aatctgcttg
     7921 acgatgacgg cacatacgac gcgattatga aggtcatcaa ggaggtggga taatggctaa
     7981 ggagtataag aagatttatt caagacccta cgctataatg cccgttatga gcggctactg
     8041 ccccggctgc ctgcactctc tggcaacaaa gattatcgct gaggctgttg aggagcttgg
     8101 tcagcaggag aacgcaatgc atattctccc cgtcggctgc tccgctctgg gacttctcta
     8161 ctggagaggc gacatggttg gcgccgcaca cggcagagct cccgctgtgg caacgggaat
     8221 caagcgctgc tgcccggaca ggcttgtatt cgcgtatcag ggcgacggcg accttgcggc
     8281 aatcggtctt gctgaaatta tttccgccgc taacaggggc gaaaacttta cagtaatatt
     8341 cgctaacaac tctacatacg gcatgacggg cggtcagatg gctcccacaa cccttatagg
     8401 acaaaagtcc accaccacat cagagggcag aagggtcgag gacgcaggtt atcccatcaa
     8461 gatggcagag ataatcgctc agcttgaggc tcccgcatat gtcgcaagat ttgcccttaa
     8521 cactcccaag ggagtaaacg aggcaaggaa aggcataaag aaggcattcc gcatgcagct
     8581 tgagggcaag ggctttacct ttatagagct tcttacaaac tgcccgacaa actggggaat
     8641 gtcacctctt aagagcctcc agtacatgac cgagaataca atgaagtatt tcgtacccgg
     8701 cgtatataag gatgtggagg tgggagcatg aaacagagca tattaatttc aggttcaggc
     8761 ggacaaggcg ttctcagcgc aggcatgctg cttgcccaga gcgcggctga ctccggcaaa
     8821 tacgccagct tccttcccga atacggtccc gagcagagag gcggcagcgc aaagtgcacc
     8881 gtcctcacaa gcgacaagga aataatctct cccctgccga aaaagtgcat gtactggatt
     8941 tgcatgaacg agcagtcctt taagaaattc ggcactcaat taaaagaggg cggcgtcctt
     9001 gtttacaact ccaacagagt tacatctccc attacaagga cagacatcac cgccattccc
     9061 gttccgtcgg accagcttgc cgaccagctc ggcaacccca aggtcgcgaa cattattctc
     9121 ataggcgctc tgcttgccgc aagaaaggca atagttgacc ccgagcactt ccttaaaagc
     9181 cttgaagaga aattcgccag caagtccgac gaaatcaggg agcttaacgc aaaggccttc
     9241 aaaatcggcg gcgacatcgg caaagattat gcggaataag taaaatttaa aataaaggag
     9301 acattgctat gcccaagtat tcattcgaaa acacaatcgg cgaacttctt gatacccccg
     9361 aaaccagagc tctcatcgac gagctttgcc ccgagcttct tgagcatccg atgctcgagg
     9421 tcggcagacc cttcaaggta aatcgggctc ttcccttcat cgagggtctt gcagacaagg
     9481 agagaatcga gaacttcaga agaaggcttg aggctatcga ataatcaaac gccaaaagca
     9541 caaagcacag cttaacgctg tgctttgttt ttttatgggc cgcggcaggg cgcttccgtc
     9601 agcggcgaac caataaaaga ataaaaccgc ccatgggagg cggttttttg aaaggaggta
     9661 tggtgcccgt ttcattgggc tatggtatga agaaaatgtg cgccaatgtg gcattgggag
     9721 gaaatatatt acatctaccg tgttgtctga actatgagaa aagaggtgtg gagaggtatc
     9781 tgtcgcctgt gtcggggagg atgacgacga tattcttgcc ctcgttttcg ggacgcttgg
     9841 agagctgtac ggctgcccat gctgcggcac ccgatgaaat gccaacgaga atgccctcgg
     9901 ttaacgctat ttccttgcct gtagcaaagg cgtcctcgtt gtcaaccttt atgacctcgt
     9961 cataaacgga tgtattaaga gtattgggca caaagccagc gccgatgccc tgaatcttgt
    10021 ggggacctgc cttgccctcc gagagaacgg gcgatgcgga gggctctacc gctacaacct
    10081 taatgttggg gtttttctcc tttaagaatt cgcccacgcc agtaatagtt ccgcctgtgc
    10141 cgacgccggc tacgaaaata tccacttttc cgtcggtctg gttccagatt tcaactgctg
    10201 tcgtagcctt gtggattgcg gggtttgctg ggttgtcgaa ctgaccgggg ataaatgagc
    10261 cggggatttc ttttgccagc tcctcagcct ttgcgattgc gcccttcatg ccgagagcgc
    10321 cttcggtgag caccagctcc gcgccgtatg ctttaagcag atttctgcgt tctatgctca
    10381 tggtgtcggg catggtgagt atgattctgt atccctttga cgctgcgacc gctgcaagac
    10441 ctatgcccgt attgccgctg gtcggctcaa ttatgaccgc gccgggtttt aatactcccc
    10501 tttgctccgc gtcctcaatc attgcctttg ctattctatc ctttacgctt cccgcggggt
    10561 taaagtactc aagttttgcg agtatccttg cctttgcccc cagcttttca gcgaatttgc
    10621 taagctctgc tatgggggta ttgcccacca aatctaaaaa acccttctta atgtctgcca
    10681 taatgtcact gcccttcata taatagtagc tgtttgtatt ataaccgttt gtaatctccg
    10741 tccccatttt aacatcgttt tgtaaggttg aatttcatct tatcacctaa acctaataaa
    10801 catatatatt ttctatgcat ttccttgtta ttagtatata gaggtggaaa taaaatgtca
    10861 atagtttttt tgaataattt ttaattaagg gcaaaaaaag agccgcagag tgcctgcggc
    10921 tttgcgtttt aaggattaag ggctctttca agccaaatgg aggctatgtc cattgcctcg
    10981 taaagacctt ttttctcgct ctttttgact attctgtcct tgggtctgac ctcggggtcg
    11041 gtattgagaa gctgtacgct tactttgtcg gcaagctcaa ggtcgcccat ctgttttgtt
    11101 tcaaggattt gcagcattat gacaaagggg tcttcgggat tgccgtaata aagcgttctg
    11161 ccgcatctta caaggggttt gcctttatat gtcaaaagct tctccttctt cttttctgcc
    11221 attagcatca cctttcccat ttttagcaaa ctcttagggt ttgcctttta caatgcccag
    11281 ataatcttta atcatagcct gaaggatttc gtccctgtca agccttctga taaggttgcg
    11341 ggcattgttg tgcgtggtaa tataagtcgg gtcttcggaa agcagataac cgacaagctg
    11401 actgatggga ttataaccct tctcctccaa ggctctgtaa acctgcgcaa ggattttttt
    11461 catctccata tcatattcgt ctcggattga gaatgtaatg gttttgtcag tcatgctgcc
    11521 acctccaaat cgaatgcttt gagacccttt acttattcgt ccctaattat attatacaat
    11581 tattatataa caggtatatt ttttttacaa ggatttttcc cgtaaaattc ccttttggcg
    11641 tcagaataca agtttttccg cgatgtaatc cttaagggag gcgatgggaa ttctgacctg
    11701 ctccatggaa tccctgtcac gaacggttac gcagccgtcg ttttcgctgt cgaagtcgta
    11761 ggtaatgcag aagggtgtgc cgatttcgtc ctgtcttctg tatctcttgc cgatggagcc
    11821 ggcgtcgtcg tactcaacat tgaagctctt tgaaagctca ttataaatct tctctgcctg
    11881 ctcgcccagc tttttggaga ggggcagaac ggctgccttg acgggagcga gggcgggatg
    11941 gagtctgaga acaactctta catcgccctt ttcagcgtcc acgacctcct cgtcgtatgc
    12001 ctcgcaaagg aatgcgaggg cgactctgtc aacgccgagg gaaggctcaa caacataggg
    12061 caggtacttc tcgttggtct cctggtcgaa atactcaagg ctcttgcccg aggtgttctg
    12121 gtgctgggta aggtcgaagt ctgtgcggct tgcgatgccc caaagctcgc cccagccgaa
    12181 ggggaacata aactcaatgt cggttgtggc gttggagtag tgggagagct cttcagtctc
    12241 gtggtcgcgg agtttcaggt tctcgtcctt aattccgagg gatttaagcc agtttacgca
    12301 gtagtctttc caataggaga accactcaag ctccgtgccg ggtttgcaga agaactcaag
    12361 ctccatctgc tcgaactctc ttgttctgaa tatgaagttg ccgggggtga tttcgtttct
    12421 gaaggatttg cctatctggc atacgccgaa gggaaccttt ttccttgctg ttctttgaac
    12481 attaaggaag tttacgaaaa tgccctgagc ggtttcgggg cggagataaa tctcgttctt
    12541 tgaatcctct gtaacgccct ggaaggtctt gaacatgaga ttgaactttc tgatttcggt
    12601 aaagttaagg gcgccgcagt tggggcagac gatattgtgc tccacgatgt acttggacat
    12661 ctcctcaaag ctccatcctg cggggtttgc gccgctgtcc tctatgagct tgtcggctct
    12721 gtggcgggtt ttgcactcct tgcagtccat aagggggtcg gagaaaccgc ccacatgacc
    12781 agaggctacc cagacggtgg ggttcataag gattgcggtg tcaagtccca cattgtaggg
    12841 gcactcgcgg acaaactttt gccaccatgc gctctttaca ttgttcttaa actctacgcc
    12901 cagcgggccg taatcccatg tgtttgaaag gccgccgtat atttcgctgc ctgcatatac
    12961 aaagcctctg cccttgcaga gagcgacgat tttctccatg cttttctcgc tgttaagcat
    13021 caatttacct ccgaacaaac ttaatatgtt aaactttaga attacactaa aaaatatcaa
    13081 gccttgccgc caaggctttt gccttaaacg cttatttgag attttatcat atattatgac
    13141 cgcttaaaag tcaagaatag cgaaaattcg ttgaaattct taacttttcc ttaattgctt
    13201 attaactgct cagctcggcg attatagtgt tggacacaag aaagccctgt ttggtaagtg
    13261 agattgagtc ctcactaatg ttcaatagat ttgtgccctt gtattttttt gctttatcta
    13321 aaaccttttc aaaacagcct ttgccgaaac gccgctctgt gtcatgacgg attagacctt
    13381 ccgcaagtct gaggcggagc ataaggtatt cctcaaagtc gcctccgctg ccgtcgggaa
    13441 taggtttgct gcctttaata tattcatcaa tatcccttgt gttgaaaaat ctctttccct
    13501 caaagaagga atgggcggag acgccgagac ctatatactc gtccaagttc cagtatttgt
    13561 aattgtgcct tgaaatatga ccctttttac aaaagtttga gatttcgtac tgctcaaaac
    13621 ccttttcctg aagatactcc gcggcgaaaa ggtacaaatc gcagacggcg tcctcatcgg
    13681 ggaggttcat tttctttgct ccctcgatac tcagcttttc ataaaatgga gtgccctcct
    13741 cagctttgag gatatacgcg cttacatggg tcactcccgc gtcggcgcag aaatcaatgc
    13801 tgtttttaag gctttcggta ttctgaaagg gaagccccag cattaaatca agggagatat
    13861 tattaaagcc cgcttttttt gcatttagcg ccgcttgctt tgcggcgtcg gcgtcggtga
    13921 gccttccgat tgcctttctt tccctcgggt ctgcgctttg aagccccagg gagatgcggt
    13981 taaaaccgct gtcaataagg cggctgaaga aatcgccgcc gcaggaggag ggattgcact
    14041 cggcggtaat ctccgcgtcg gggcttatat caaatacttt tctgagatgc aaaaggatat
    14101 cggacagtct ttcggcacca atgaatgacg gagtgccgcc gccgaaatag actgtatccg
    14161 cttttatttt gtttttcctt ttatatccct ccgcctcccg ctttaaggcg tctacatacc
    14221 tatctattat atcgccggag ggcactacgg agcagaagtc gcagtagtgg catttggact
    14281 tgcaaaaggg gatatgaaca tatatcccct ttttgtcttt actgttcatc gagcttgagc
    14341 accgcaagga atgcctcttg aggcacttcc acagagccga actggcgcat tctctttttg
    14401 ccctctttct gcttttcaag cagctttttc tttcttgtaa tgtcgccgcc gtagcacttc
    14461 gcgaggacat cctttctgag agcctttacg gtctcccttg ctatgacctt gccgccgatt
    14521 gctacttgaa tcggcacttc gaacatatgc ctcgggatgt tttctttcag cttctccgcg
    14581 attcttcttg ccctctggta tgccttttcc tcatgaacca tgaatgagag ggcgtctata
    14641 acctcgccgt taagaagcac atccagcttt actatcttag actgctgata gcccgccagc
    14701 tcgtagtcaa gagaggcgta gccctttgtg cgggatttga gagcgtcgaa aaagtcgtag
    14761 ataatctcgt taaggggcag aatataacta atatccacac gctcgggggt gatgtaaacc
    14821 atgtccaggt acactcccct gcggttttgg caaagctcca taatcgcgcc aacatagtcg
    14881 ggcggggaga aaatatgcgc ccttgcgata ggctcctcta taaagtcaat gtccgcgggg
    14941 tcggggaagt gggtcgggtt gtcgacctct attaccctgc cgtctcttag atgcaccttg
    15001 taaataaccg cgggaacggt ggagataagg tcaaggtcgt attctctttc aagacgctcc
    15061 tgaacgattt caagatggag aagtcccagg aagccgcatc tgaagccaaa gcccaaagct
    15121 cccgaggttt cgggctcgaa agataaagcg gcgtcgttga gccttagttt ctccaaagcc
    15181 tctttaaggc tttcgtaatc ggcgccgtcg gcagggtaaa tgccgcagaa aaccatgggg
    15241 tttgccttgc ggtagcctgc caaaggctcc tttgcggggt tgtttactaa ggtaatggta
    15301 tcgcccacgc ttgcctgact tacggtttta atggaggcgg ttatatagcc tacctcaccc
    15361 gcggaaagtc ccttgcaggg ctgcaggctg gtggggcgca tataacctgt ttcaacaacg
    15421 gtaaaagtag ctcccgtgga catcattttc atggtgtcgc caggctttat tgagccgtcc
    15481 attatcctta catatacgac gactcccttg tagctgtcgt aataggagtc aaatataaga
    15541 gcctttaaag gagcgttttc gtcgcccttt ggcggtggaa ccagctttac aatgttctcc
    15601 agaaccgcct ccacattctc gcccgttttg gctgagattc tgggagcctc ggagcagtca
    15661 agacctataa actcctcaat atcccttgcc accctgtcag ggtcggcggc ggggaggtcg
    15721 attttgttta taacgggcac taattcaagg ttattgtcaa gggcaagata tgtatttgcc
    15781 attgtctgcg cctctatgcc ctgggttgcg tcaacgatga gcaccgctcc ctcgcatgcg
    15841 gcaagggagc gtgagacctc atagttaaag tccacatgac cgggtgtgtc tataagattc
    15901 aaaacataat cattgccgtc ctttgccttg tactcaaggc ggacagcgtg cgctttaatt
    15961 gtaattcctc tttctctctc caggtccatg gagtcaagca gctgctgggt catctccctt
    16021 gactgcacgg aatttgtaag ctcaaggagc ctgtccgcaa gggttgactt gccatggtca
    16081 atatgggcaa taatcgagaa attccttata aattccttgt tgctcatata tcgtaccttt
    16141 cattttaatt ctcagattac atcgccccgg tggggtttaa agccgagtcc gaaaacatcg
    16201 cccgcctggg atactatgat aaacgggttt tcgtctatgc tgcgggctat tctcattata
    16261 agggcgacct cgtttgccct tgttacgcaa aggagcatgc tcctctcttt gccggtatag
    16321 ccgcccgtga cggggagaat ggtaacgcct cggctggttt cgtttgtgat tgccttcgca
    16381 atctcccgac ccttgtcggt cacaatctga atcatcttgt tgtttgtggt gccgtacaat
    16441 aggtagtcga ttgccttgct gctgatgtaa aacgcgatta tggcgtagag cacgctttca
    16501 atgctcttgt aagctatgcc tgccacaatg accaccatca tatcaaggac cagcataaag
    16561 ctgcccattg atatgtgggg gaactttagt ttcataagtt taccgattat atccacgccg
    16621 ccggttgtgg cgcctcggat aaaaacaagt cccagacccg ctcccgagaa aactccgcca
    16681 aagagggcgg caaggagctt gtcgcccgaa tagacgggga gaaagcccgc cgtaatgtct
    16741 atcactaccg acgccaaaac cgtcgcgaca acggttctga acaaaaactt tttgccaaag
    16801 aagattaaag acaaaatgaa aagagggata ttaaggagaa tgaccgttgt gcctatgggc
    16861 aaaccaaaga ggtagttacc gacggtcgct agacccgtta ctcccccggg agcaatgtgg
    16921 tttggagcgg taaagcagtt gaccgaaatc gcgtaaagcg cgcttcccac aataaagaaa
    16981 aacgcgtccg ccacaagctg cctcataggg tccttgggag tgctattcgt tctctttttt
    17041 attgtctttt cgctcattta tatccctcat aataatatcg aataatatgt agagcctatc
    17101 ggtctttccg ctgaggtaca gatagccgaa caatgcctct aaggcggtgg cgtagcggta
    17161 ctcctccgcc gatttattct tggggatatg cgcgttatga gcgttgcgtc cacgcaggaa
    17221 aatttgacgc tcatcttctg tcagataatc aaaaatcact tttgccgccc tgaactgggc
    17281 tgaggcgttg acaatctcca gtttgtcaag atgcagcctg ccgacggccg actggctgtt
    17341 tgccacaata taagagcggg caagagcctc gtaaaccgcg tcgcccataa aggcaaggga
    17401 ataggtggac atctccctcg gggatttttt tagtttaaag gcgtctgggt taagactgag
    17461 ctcggggaga tttatgcttt tatcctcagc cttttcgggc tctaatgcgt ttgttttttt
    17521 attctttcta ttaaggaaca taatcgaact caaggtcgta gattttgacc gtatcccctt
    17581 cctgaattcc cattctttca agctctgcga ttatgccgct ggactggagc accctttgga
    17641 aatactgaag ggactcgtag tcgtcaaggt ttgttttgtt aaggatttta agcagccact
    17701 ccgcctcgat gatataaatg ccgttctgct gcctgatgtt aaagccctcg gactcgccct
    17761 ttttgcgaag agcttcaagg ggaacctcct cagattcgta aatcttgatg ggcgggagtt
    17821 tgtcaagcat tgccgcaatg cagttaataa gctcctgagt gccgtgggcg atggcggctg
    17881 acattgtaaa gaagggatag ccgcactcct cggccttttt tctaagcctc tctatttgct
    17941 cgtcctcggc aaggtctatt ttgttgccca caacaatctg gggcttgttt aacagctctt
    18001 tgttaaaggt ggcaagctcg gcgtttattg tatcaaaatc ctcaacgggg tctctgccct
    18061 cgctgccgga aacatccaca atgtgaacca gcagcctgca gcgctcaatg tgccttaaaa
    18121 atgtgtgacc gagacctacg ccctcgcccg cgccttcaat aagaccgggg atatccgcca
    18181 tgacaaagct cttgtcgccg acggacacga cgccgagaac gggggttatg gtggtgaaat
    18241 gatagtcggc gataaccggc tttgcggcgc tgaccaaaga caagagagtg gatttgccaa
    18301 cattggggaa gcctatgaga cccacatcgg caagaagttt aagctccaaa ataagctccc
    18361 actcctcgcc gggagtgccc tcacgggcaa agcggggagc ctgccttgtg gaggtggcaa
    18421 aacgggcgtt gccaaagcct cctctgccgc ccttcgcggc cacaaacctt tcgattttgg
    18481 acatatctgc gataagcgcg cccgtctccg cctctttgat tatggtgcct cgcggaacct
    18541 taataataag gtcgggagcg cttttgccgt tgcgcttgtt gcccgagccg ttttcaccgc
    18601 tctgggctat gaacttcttt ttatgcctga aatcataaag agtggagaga ttgtcatcca
    18661 caacaaaaac cacatcgccg cccttgccgc cgtcaccgcc gtcgggaccg ccggcaggga
    18721 catacttttc cctatgaaaa gcaacggcac cgttaccgcc gtttcctgcc ttaactttga
    18781 tttttgcgga atctataaac atgcttctct cctgcttaca gtaaaatttg cgctttcaga
    18841 ttattgtatc atgagaggaa gtctttggca accgaaaatt atctctgccc ttgatttata
    18901 tagggaaaga gctttatgaa aacttctttt tatataagga attgagatat tcgtccccgt
    18961 aatagtcgga taaatcatct tcatcggaat ctatatctga aactgcgtca acaatatgag
    19021 cgtagctttc ctcttcaaat ctgtttttag gaggcgtttg ataaagaccc gtagcgtcaa
    19081 agaacgaaac ggcacccatt ctgcttatat cggggttttt aagtttttta atccttttat
    19141 acatggtttt tcctcctttt atattttgtt taacttagta tttgcactaa aggtccgatt
    19201 ataaataggg cttatagttt cataagtttg ccttaagatt agcgtttaaa ttttataatg
    19261 gatttaaaga ggaaaaaccg aaaatgtttc tgcgtgactt tgccccaaaa aaatttatct
    19321 atatgcggct tgtcctgttt ttatgaaaag aggacatata taaagctttg attattaaat
    19381 acagtgttta ctttttgttg catttaaatg catgagataa tgaacggtat ataccgttga
    19441 atattttccc taaaaattac attcagaggg tgtagaaatt cgtgtaaagt ttttttcttt
    19501 acaatttttc gggctatttt tcctaattgg aaaaacaacg ctgaaatttt gaacattttc
    19561 aacagagttt tgaacatacg caagtaaata gctttacatt aaaatttttg cccttatcgc
    19621 atacgaaagc agagaagcgc ataatattta attgatttcg caagtttttg tataaatata
    19681 ccgaatattt tctaatattt tccgtatgat ttctaaattt tttaaaacga atcttgtcgc
    19741 aaattgcgtt ttatttttgg acaagatttc cccggaaaag cattattaga agatgacgca
    19801 accgaggaaa acttgccgct aagaatcgcg caatagaaat gattttaaat ttaaacgcaa
    19861 aaaaagcggc gcccgaaaga gcgccgccgt caatttttta agtttggttt acttcttcag
    19921 tacagccttg tggaatacct ttttgccctt ctttataatt acatgaccct tttcaaagtc
    19981 ggaggctgag aggcttgcga aggtgtcgga gattttttcg tcgtcaaccg tgatgccgcc
    20041 ctgctgaata agtctccttg cctcgccttt ggagggcgca agctttgtgg cgacaagcag
    20101 gtcggcgacg gaaattttgc cgtcaacgaa caaatcctca gtcagctcgg tggtgggcat
    20161 gttagcgtcg gaaacgccct tttggaacag agctcttgat gcctcaagcg ccttctgagc
    20221 ctcatccttg ccgtggacaa gggcggttac ctcgtaggcg agcacctcct tgactttatt
    20281 cagctcgctg ccctcgtact tttcgtactc ggctatctgc tccacgggca caaaggttag
    20341 catcttaagg cagttgatga catcgctgtc gtccacattg cgccagtact ggaagaactc
    20401 atagggcgag gtcttttcgg ggtcaagcca cagggcgccc ttttcggtct tgcccatctt
    20461 tctgccgtcg gaggtaagca aaagcttaaa ggtcatgccg taaacctcag cgccgtccgc
    20521 ccttctgtta agctcaacgc cgcctatgat gttgctccac tggtcgtctc cgccaagctg
    20581 cataatgcag ttgtaatgcc ttctgagatg caggaagtca tagctttgca ttatcatgta
    20641 attcagctca aagaaggaaa gtcctctttc aagcctttgt ttgtagcact ctgcggcaag
    20701 cattctgttg acggagaagt gaacgcccac atctctaagc atatcgacat agttaagttt
    20761 cagcagccag tcggcgttgt tgactaaaat agcctttccg tcggaaaagt caacgagtct
    20821 tgacatctga gtcttaaagc actcgatatt atgctggatt tcttcttttg aaagcatttt
    20881 gcgcatttcc gtcttgcccg acgggtcgcc gataagaccc gtgccgccgc cgaaaaggat
    20941 aatgggggtg tgaccggctt tttgcagatg ggagataacc ataatctgca caaagtgtcc
    21001 cacatgaagg ctgtctgcgg tggggtcaaa accgatatag aacctgattt tatcttttgt
    21061 cagaagctct ttgatttttt cttcatcggt catttgagct ataatgcctc ttgccatcag
    21121 ctcgtcaaaa acattcatga tattgtcctc ccgtatatta aaagcttata gtctatcaaa
    21181 aaagccccct gcaaaagcag agggcgaata accgcggtac cacctcaatt tgatgcgcaa
    21241 ggcatcctct ctgacggcgt cgaaaaaccg attaccgtcg cagcctttaa cggggctaat
    21301 tcgtctgctc ctactgaaac tctgagtttg ttcagagcag cggctcggaa atgtatttcg
    21361 cagccggctc acgctttctt ccaccaaccg aaagctctct atgcatcacc catgctgtta
    21421 cttcttttcg tcatcgcctt ttgctgttta gttgtaataa tattatagcc atggatttcc
    21481 ttttgtcaat attgttatag ccgtttggct caatgaagct ttgcaaatca gtcattgaaa
    21541 tagcttttga ttttttcgta gatttcgact attttaaagc gcacctcgaa tttggggttt
    21601 ttctctatga gtctggctcc cccgcttgtg agaatcgcgt cgattttcac atctttgtcg
    21661 tcatgctcct cggcggcggg gagacacaga ggcgggaaca ttacgcacca ccagtttttt
    21721 ccctcgccgg agcctattat aactttgaca gctctgtatt ttcccgcggg cagagttttg
    21781 tcctcatagc ttctggtgtc aaaatactct tcggtaagtt ctaccttaac atcataatta
    21841 tagcccattt cccttgcggt atcctcggca agggtctcaa ggcgctcggc ggcggctgcg
    21901 attttctccg ctgcgctgtc agcgtcttcc gcgcccgcaa agatgtccgc tccctcggtt
    21961 aagagcttat cgcggatttt gagtttaaga gcctggtcct cctcgctgtc ggagttggca
    22021 aggatatgca gcctcaacac attatccctt acctctttgc agtttgtgct gaacgccgcc
    22081 gtgctgatta tgcaggaaat aagtataagt acaatgaccg cagcctgaac aaagccgttt
    22141 ttggaaagct ttgaggcgct tttattaaat gccgacaaaa ccttattttt cataatatcc
    22201 tccgatttta aacaatatgt acactgagag tattaacaga aataggtaat ttattcctgc
    22261 attagcaaaa attatgcata aagacgctcc ttaaaaagag ggagcataat ttttcttact
    22321 gaggaaaata taaaaacaga aaatcagact aatttcggca aagcaatatt tggagtctga
    22381 ccttctaaaa aaggcgcagg gagaagattt gccaaatttt aattcaagca aaatttgcat
    22441 tataggacct tcgcggcaaa aaatatcaag gataatactc gaaaggagtc ttgaaagtga
    22501 aacacggttt gtcaagacga ggcaaaatca gaatcatctc atttttgtca atactggtgc
    22561 tcgttctgtc catttggggc ggcattgaga catataaaag gcgcacatat cagcagcttg
    22621 taaaaatatc tcaggagcgc gctctggcag agctgggcaa ctatgttacc aatataagcg
    22681 ttgcgcttca aaagggcagg tatgccaata ccccgccgat gatagcgaat cttgcctcct
    22741 cgctttggag agaggctacg ggagctaaga cttgtctttc tcagcttgaa agcggcggaa
    22801 ctcagcttta caacacctac aaattccttt cgcaggtcgg cgaattcacc atggcgctga
    22861 acagaaaggt ggctgccgat gagcatatta ccgatgagga aagaaaacag ctggacgagc
    22921 tgcttaaata cgctcagtcg ctggccgacc agattaacta tatgcagtcc atgtacgaaa
    22981 acgacgcttt gacatttgac gctaaaaagc agggactgct cttaagcgac gcggcggata
    23041 cccagaaact gagctatgtt gagggtatcg ctaacgcgga gcagacaatg accgactatc
    23101 cgactctgct ttacgacggt cccttctcgg acaatatcga ccagaaggaa tccaaactct
    23161 taaaggaatc cgaccccgtt tcaagggagg aggcaagaaa gattgcggcg tccttcctgg
    23221 gagctgaccc gtcgcaggtt gcggacgcgg gtgatgagga aggcaaaatg gccgcgtatc
    23281 tgttctccta tgaatcaaag gttataaaca tcacaaagaa gggcggcttt atctcctata
    23341 tgctttgcag cgattttgcg ggcgaggaga agattgacca gcgtcaggcg gttgatatcg
    23401 caaagaaata ccttgaaaaa cacggctaca agaatatgaa ggagagctat ttctccacca
    23461 acgacggtat ttgcaccatc aacttcgcct atatggaaga cgatgtggtc tgctaccccg
    23521 accttatcaa ggtaaatgtg gctcttgaca cgggcaatgt ctgcggcatt gacgcaagag
    23581 gctatataat gaatcaccac gaaagaaaga ttcctgagaa tatcgtaagc tccgacaagg
    23641 ctaagcagtc gctgagcaag aacctcacag ttcaggatgt tcagcttgca tttatcccca
    23701 aggacaacgg cgatgaggag ctgacctatg agttcctttg caagagcaag gatgaccagg
    23761 atgttcttgt ttatataaat gttgactctc ttgaggaatc cgagattctg atactcctct
    23821 acaccgacgg cggcatgctg acaaaataac cgcaattggt gtcgaatttt gagaataaag
    23881 aaaatttaac agggcatact ttgatgggat atacccacac acagaaacga aaggaagaat
    23941 acaatgaaaa agactattgt gcttctttgc gcacttttaa tagcctttgc ggtgctcttt
    24001 actgcctgca acaaagccgg cgacaaggat aagatgacca tggacaacca gacaaccaca
    24061 cagcctgccg gcaacatcac aaccgcaaac gaaaccacct ccaagggcgc ggtagaaagc
    24121 aaggcagagg aaatcggcac aaccattaag gataaccttg aaagcattgg cgaaggcgtc
    24181 tccacaaacg cataaaaaag cctcccgtat aaaacgggag gtcatcaatt ctatcagata
    24241 agtctataat actgaaatct cactaaatat aaacctccct tttcgggagg ttttaattta
    24301 tgcggttaaa aacagatatt tacttttgag gcgaggcgaa aatctgagtc cagtatgtat
    24361 cgtaaattgt tgtggtgtca ttcgtaaagc caacgccgat tgcggtaaac tccgcccgca
    24421 gtatgttggc tctgtgactg tgggaattca tccaggcgtt taccacatcc tgcggagtgt
    24481 cgggacccgc cgcaatgttc tcgcccgccc aggcgtattt aaggtttctg tccttcatca
    24541 tcttcagggc gattccctct gatatgtaat tgcccttgtt gtcaaaataa tgcctgaacg
    24601 gccagccgtt tgccatatca acagaccttt gtcttgcgat ttcggaaagc tcgaagctga
    24661 cttgaagggg cggaagattt accttggccc tttccacatt tacaagcctt atgacctcat
    24721 attcaaaatc gctcaaatcc tcatactttt tgttggggtt gtcaaaggga gcgtacaagg
    24781 gcgctctgag gtgggcgttt atgttaccct cttgtttcgg ttctccctca tcatcgcctt
    24841 cggggattct gggaacggcg caggcggggc gatcggagtc caagacgaaa accgttttct
    24901 gcggcagtgt ttcaagtctt gcgcagtgcc ttaaaatcag ccttgcgtcg gcggcgttga
    24961 ttgtcgtatc cttgtcgttt acatttatca catcaaagta cgagccgtgg tactcctcca
    25021 gccttgcgac gattctgaga agtatccttg cgtccgcggg ctgtactttt ccgttaaggt
    25081 ccgcgtcacc cacgctataa tagcctccgc ggctttcgtt tgccttgccg tatgcgccga
    25141 gaaccttgcc gtaaggcata aaaagctccg agaccttagc ctcagagcca ttttcttcca
    25201 cgctgttttg ctgcgggaca gttgggacat atttctcctt tataagctgg agctttgcgg
    25261 cgtgtcttag gattaatctt gcgtcctgag cgtcagtctt gccgtcgccg tttacatctc
    25321 cgctcaaata aatgccttcg ttagacttgt ccagcatggc ataaactctt agaacctgcc
    25381 ttgcgtcctg agcgttgatt ttgtcgtcgc tgttgacatc gcccacaaga atcgggttcc
    25441 agttaaagtc cttatactcc tgctcaacta tataaagtga cttgtcaaaa atctcggcgt
    25501 tgctgaggtc aaggaaaaca aaggcctcag ccacaaggtc cttaagaccc aggttaatct
    25561 cataatgggt tttgtagtat gtaagctgac cgtttccgtt tacagtgcac tctgcatagg
    25621 cattctgata tttgaacttt gcgtctttcg ggtcaaagtc ggaaactacg gtctttacca
    25681 catttgccag cttctgagtg tcaatcaaat cgaaaactct gcccatgggg ctgttttcgg
    25741 tggggttaat ttcatcggca aacttaatct taattgtata tccgccgtta aactcgggat
    25801 aaaactcata ctgataaata tcttctttcg taagcgcgga aacatagggc ttgcccgtta
    25861 ccgatactgt gttctcggcg ggtatgcctt taattacgaa ttttgcgttg tcggtgagag
    25921 aattgttaag gataaagggc cctgtgattt tgtttaccaa atcatcgatt tgagacgcgt
    25981 tgtcgctgcc taaaacatcc ttcagctctt tgccgaggag gttaaagaaa aactcgccca
    26041 agatgccgcc gaggaaattg tcaaaaatgc cgctgtccaa aaccttgcct accaaatcat
    26101 agtagactct gaccttttgg aaatgatagg ctatatcgtt attgtcggtg cctgctatgc
    26161 cctgcctttt tattacattg taggcgggtc ttttttcttt aattgtgttt acgctgctgt
    26221 tgaaaagctc cgcaataccc tccattccgg ggtcttctat gtacgcagcg gaggcgcagg
    26281 tgctaaaaat catgccgaaa gcaagaatta aagctataac gcccgttgtg attttatgta
    26341 tccttttcat ttcacacctc aaagctctga gcagttttct tcatattctc ccttgtacta
    26401 acaaaatatc atattatgca aaaaaaatca aagaaaagtt ttgttaagtc ccttccctcg
    26461 ataattttcg caaatcttcc aaaagcaata aaaaccttgt taaatctcta ttcctatgat
    26521 aaaatgaact aagtctaaaa ctttacatat ggagattcta tgaaacttga atatagagga
    26581 attataaaaa gtgttgaaac cctgcctaaa accgacctgc ccgaaaacgc ggtgaaattc
    26641 aaagagcccg aaactcttgc cgccgtaaac aaggccgcgc ttttttatat ggttcctctg
    26701 gcagcgttta tttgtctttg cgtctatttg ggagtaaagt tcgggggcaa aacgaatccc
    26761 cttgatacgc ctttgtatcc gggagcgatt ctcgctcttt tgtcggtgct cccccatgag
    26821 ctgctgcacg cggtatgctt cggcaaggac gctgtggtag gtctttactt tgctccaaag
    26881 gagcttatgg cttttgtttt ctgctcaaag cctatatcca aggcaaggtt tatattttta
    26941 agcctttgcc ccaatttggt tctcggctgg ctgcccttta tgctctggct catattgccc
    27001 gacttaggaa aaacgggcag caatcttctt atattctccg tctattcaat tctgatgggc
    27061 gcgggagatt ttatgaacgc ggtgaacgcg ataaggcaga tgcccaaagg cagctaccag
    27121 cagctgtcgg gcatgcattc atactggttc atgccgtaaa tcaggcaatt atgtgcgcgg
    27181 cgaatgcccc gcatttttga cataaacctt atggaggtaa aaacaatggc agacaatatt
    27241 cttgaccgct ttgacaaagt gttcacggaa gaaaaggaag ttgacgcttc caaaaagatt
    27301 aaggtcggca tcatcggctg cggctggatt gcagaggctc atgtaaagag ctatcttaaa
    27361 tgccccgatg ttgagatagt tgcgggcgca gaccttatcc ccggcaaggc agagaaattc
    27421 tttaaggacc aaggcattga aaatgtacgc tgctacaaca gccacaaaga gcttatcgac
    27481 aacgaggagc ttgacgctgt aagcgtttgc acatacaacg ccacccacgc tgagtgtacg
    27541 atttatgccc ttgagcacgg tgttcatgtt cttcttgaga agcccatgtg cgtaacactc
    27601 gaggaggctg tcgaaatccg cagagctgag aaaaagtccg gcaaggtgct ttcaataggc
    27661 ttccagcccc gctttgacga gaacatgaag atggttaaga gaatcgttca gtccggcgag
    27721 ctgggcgagg tttactacat acagacaggc ggcggacgca gaaggggcat ccccactccc
    27781 tacggcacaa cctttattga gaaggagacc gcaggcatcg gcgctatcgg cgacatcgga
    27841 tgctactccc tcgacatggt tcttaacgct gtgggctatc cgaagccgct caccgttaca
    27901 ggctatattt ccgacttctt cggcaagaat cccaagtaca tggctaagcc cgaatacgca
    27961 gatgttttcg gagttgatga cttcgctgca ggctttatcc gtcttgaggg cggcataatc
    28021 ctcgacttcc gtatttcctg ggctatgcat cttgacaccc ccggcgatac aatcatcctc
    28081 ggcaaggacg caggcctcag aattccgtca accgactgct ggaacggcag catcggcggt
    28141 cccttgaagg tttatcatga tgtcgccggc gaatgcgttg ttacagagtt gcccatggca
    28201 aagaacaaag gcgacaactt tgacaagaag attcgttcat ttgttgacgc tgtcaagtac
    28261 aatctgcccg ctcccgtacc ctctgagcag attatcatta accaggccat cattgacggc
    28321 attgttaagt ccgcgcagct cggccgcgaa atcgcaatcg agattcccga aatataatgt
    28381 cattgcgccg cttcatgtga ggcggcgcgt ttctttcgct gccggagggg ttttcctcta
    28441 agggaagctc ccctcccggc ggacggcgtt cgggagtcag cgggcagttc ttcgcttgcg
    28501 tcagaggagg gattatctcg ttgggcgtaa ctcatctctg cgtgagcaga cgggcgcacc
    28561 ggggcggtcg tcgtggtcga ccgcagtatt cgacaggttt ctccctctgc gggcggcaga
    28621 gagaatgaaa accttaattt aggattttta aaagcaaagg aaaagcattt ttcgaatcgc
    28681 tgacttcgga ttgccataat attatcaaaa tttactttat gtttgcatat tgactgaata
    28741 aaaaaacatg tttataattg tattaggaaa ccaatttttg gcgtgctctt ttctttgccg
    28801 cggccttatg cgggaatact aaaaaaaact atttgttttg gagtgtttaa attgtcaaat
    28861 atcaaaaagt tttcgaggga acaaatagcg gaaattgaat gcgcaatgcg aagtacaagg
    28921 tcaaagaacg agtaccgacg cctactatgc cttcttttaa gaatcgagca caaaatgcct
    28981 ttggatcagg tttctgaaat cgtcggcttt aacaaatgct acataagcaa actggtttcc
    29041 cagtacttaa agcatgggct tgacgccatt tccgacaagc ctatgggcgg caacaggcgc
    29101 aatatgacct atgaggaaga agaagagttt ttaagaccgt tttttgaaag gcgcagcaag
    29161 ggcgaaatcg tcactgcagc cgaaattctc aagaaatatc gggaggttac cggaaccaat
    29221 gctgcctcat caacaattta caggctgctt gacagacaca actggaaaag ataaaaaaca
    29281 aacagaattt ctttaagccg tactcgaggg gtgaatcgag tacggcttat gttttgccat
    29341 ggattctgat aattctgctc aaaattattg ccaatattta caaaatttat gttgaaatca
    29401 gttaatttta tcgatataat attatcgaat gtcaaaaaaa taacaaaacc tgcaactcaa
    29461 acaatgaaaa accagaggag gagccagcat gaagtatgaa gaactgctga aacggctgtc
    29521 ggaggatttc ggcgactggt ggaaaaaggg ctatgagatt cccgaaatga caaacaatct
    29581 ctacccttac gaaaagctgt tttcgcctat cagggtcaac agcattacca tcaagaacag
    29641 gcttgtaatg ggtcccatgg gcaacatcag catgtgcgag gaaaccggcc gtccgaacga
    29701 caagatgctc aagtactttg aggaaagagc aaggggcgga gtgggcctta tcacaacagg
    29761 tcttattccc gtatccttcg gaattgacaa gtcgctaatt gagcttggcg agctttccta
    29821 cttcccccgc attgaccgtt caaggacagt attcgccggc tggagggacc ttgccgccat
    29881 gtgccattcc cacggcagca agatatttat tcagctcact ccgggtcttg gcagagtggg
    29941 caatccccag tgccttgtca atcagaagaa attcccccgc agcgcctctt tcaatcccaa
    30001 ctggtatatg aaggaggtac cctgcctcag actttccgac aggagcctga gggcaatcat
    30061 taagcgcatt ggtcaaggct cggcggacgc aaaggctgcc aacatcgacg gcgtatatct
    30121 gcacggtcat gagggctatc tccttgagca gtgcaccaac cccgccttta accgcagaaa
    30181 gttcggcagg tacgcgaact accagaactt cggtcttgat atgataagag aaatcaggcg
    30241 cagggtcggc gacgactacc cgattatgta ccgcatagac ctcagtctta tgctgaacgc
    30301 tacatacggg gacaagatga acaccgtatc ccccttaaag aaattcaaga acgagagaac
    30361 ggtcgcccag acacttgaat atatgaagaa ccttgtaaaa gccggcgtag atatgttcga
    30421 tgtggattta ggctgctacg acaactggtg gctgccccac ccgccgggct ccatgccgtc
    30481 gggctgctat cttgacattg caaagctggt taaagactac tttaaggaga acaatatcct
    30541 ctccaacaag ggacttgagg ttcccgtcgt ggctgtgggc aaactgggct atcccgacct
    30601 tgcggaaaag gctctcagag aagaaaagtg cgacatgatt atgcttgccc gcccgctgct
    30661 tgccgacccc gaatggtgca acaaagcctt cgcgggcagg acaaaggata ttatcccctg
    30721 cataggctgt caggagggct gcatcaacga gtttgttgag ggcggtcatc cccagtgcgc
    30781 ggtaaatccc agaaccgcct ttgaggatga gttctcaagg gagattccca aagcggaaat
    30841 ccagaagaat gtcgccgttg ttggcgcagg tccggcgggc attaccgcgg ccggagtgct
    30901 ccttgagaga ggccacaagg tcactctgtt tgaaaaggaa agcgttgtgg gcggcaacat
    30961 cgtacccggc agttacccca agattaaata cgagataaag aactaccttg cataccttac
    31021 tcactgcgtt gaaaagtatc aggagaaccc gaatttcacg ctgaagctga ataccgctcc
    31081 cactgcttct gagcttaaag aggcaggctt tgatgtcatc atcatagcat gcggcggcac
    31141 tcagactcgc ccgcccgtaa agggcatcga caagcccaat gtctactcgg caattgatat
    31201 tctgatgaaa cccgagctga ttaaggacgc caagaaggtt gtcgtaatcg gcggcggcgt
    31261 tgtgggcgcg gagactgcat acttccttaa atacgagcac aacaaggatg atgtcaaggt
    31321 cgtggagatg gaaaagtaca taatgaacca cacctgcaca gcgaacaggg gtcatattat
    31381 ttactatctt gaaaaggctg gcgtggagct gcttaactgc acgactctta cagaggtaac
    31441 cgacaagggc gtaaaggtac gccagaacac acataagaat gtgccgaacc cctatgtaac
    31501 atggtcgccc attctgcccg agaatgtgga aaatcccatg gacagattca gcaagataaa
    31561 agacgagcac aaggagagat tcattgagtg cgacgcggtt gtggtcgcga cgggcgtaaa
    31621 gagcgcggat tccttgttct atgagtgcca gaaagtcttt gcagctcccg aaatctacaa
    31681 tgtgggcgac agctttaaga ccgcaaagat atttgaggcg acaaggagcg cgtacagaaa
    31741 ggcccgctca atctaatctc tcaataagga aaggtggttt gcattatgag tactgtggaa
    31801 acccgcgccg gcgagtataa aatggagctg actcaggagc agcttgacat actcgaaggc
    31861 aaaaagggcg aaaccatggc aaaggttatt aaaaccgttg tcatgttcgg cgaaatcttc
    31921 ggcgcaaagc gtcttgtgcc catcacccac aacagcggac accttgtaac cagcttcggc
    31981 ataggcattc tggctcccct ttacagaaca atggatgagc tgataaacgc gggaattaag
    32041 gtcaagggcg actttaccgt tgacccaaga cccctcgact acaagaatgt caaatgcacg
    32101 atgcttgaga aactgctttt caacaagatt ctttattcca agcaggagca gtacgaatcc
    32161 cagctcaaaa aggtaggctt aaaggacgag gacggcttta catgcacctg ctatctgccc
    32221 gaggtgggca acacccctaa ggagggggat attctcgcat gggcggagtc cagcgcggtt
    32281 gtatacgcca actccgttct cggcgcccgc tgcaacagaa acagcggtat gctggatttg
    32341 ttcagctcaa tagtcggcta tgttcccgaa tttggtcttc ttaccgatga gggcagaaag
    32401 gcaagctgga aggtatatgt aaacaccacc aaaaagcccg aggctcaggt tctgggcagc
    32461 gccatcggca tgaaggtcat ggaggatgtg ccgtatgtct atgggcttga caaattcctc
    32521 ggcactgagc tgaatgacga gaacaagtca taccttaaag acttcggagc cgcgaccgca
    32581 agcaacggcg ctgtgggtct gtatcatatt gacaatctca ctcccgaggc aaaacgctgc
    32641 ggagaggagc ttataaaacc cgacgctaaa gaatacataa tcgacgacgc ggagctggag
    32701 cgcattaaaa actcctaccc gataatgtgg aaggacccga acaaaaaggc ggacaaatgc
    32761 tttatcggct gcccgcatct gaccttgcag cagcttaaag actggacgga taagattgag
    32821 aaaggtctta aagaaacggg caggaaaaag gtagctttga ccaccgtttt caccgccgct
    32881 cccggcgtgg ttaaggagtt tcaaaagagc gagcattacc cgaggctaat ggctgcgggc
    32941 gcaaagcttt caagcatatg cccccttatg tataccaaca atcctcttac aaagaagtac
    33001 aatatcgcca ccaacagcaa caagctgagg acctactcgg cggcaagatt ctatgaggat
    33061 gatgaaatgc tggcaataat cgtaggaaag gagggccact gatgaaaagc tttaaaggca
    33121 gagttatcgc gggaggaaat ttcaagggcg aggcggttgt ttcaaggcag ggacttaaca
    33181 ctctcgccac cttccaaaaa agcgcgctca caaggtctaa ggaggttata ggctccgacc
    33241 agaacaaccc cgatgtattc ggcaaaaagc tgacgggcaa ggttctgtgc ctgccccaga
    33301 caataggctc cacaactggc ggacttgtga ttcagaccgt atgcagcctt aaagcaaacc
    33361 ccgcggcctt cctgttctcc gagcacatag actcccttgc cgcaagcggc attgtgctgg
    33421 ctaaggtatg ggaaaacagc gatgttattg ccattgacct tctcggacag gagttcctcg
    33481 ataccgttaa aacaggcgat acggtagaga ttaaagagga cggaacggtg actatactct
    33541 aaacaaaagc ggaggttcgc ctccgctttt gtttgccccc gttttcgggg gctttttatt
    33601 tcataagacc tccttaaaac tcatatattt aaaggtaaag ctttaaggag gtggggctgt
    33661 gataaaagag gacatcctga aaaatatgta tcttgccggc tctgcctact ctcagaccca
    33721 ggccgatgtt ttcggcggcg gggtgagagt tatagacgac gcggaaagcg ggattcagtg
    33781 ctttttaaga agggacggcg gaacgctcta cattaccttc agaggctccg actcccccaa
    33841 agactggcag accaatcttt atttcagaag gctggttatc ccttacggca acacaaagtc
    33901 aaaaatcaga atgcacgctg gctttatcta cgcctacaat aacgacaggg taagacccca
    33961 gcttcatagt gccgcaaaga acaccgacaa gatagtgatt tcgggtcact ctttgggcgc
    34021 ggcgctgtca atagtctgcg cggttgacat tcagtacaac ttccccgaaa aggatattga
    34081 ggtttacgct tacgggggtc ccagagtcgg caacctcgcc tttatccgct cgtacaacaa
    34141 gagggttttc aaaaccttca gagtggaaaa cggcaacgat attgtgacaa agctcccccc
    34201 tgcgattttc ggctaccgcc acgcgggcat aaaggtgcat gtggggcaaa tgaggcttcc
    34261 gcttgtattc tcctttaatc agcacagacc cgaaaactac gcaaagaaca tcatcaggga
    34321 tttttacaac acaagttaat tccttattga acagtcgacc aaaaaagagt ataatttaac
    34381 aaatcgaagg agtggcttcg ttataaattg gaggcagtaa caatgaaaca tacatatata
    34441 tctaaatcgg ctctgagggt tatttccttg atccttttac tctccatagc attcatcccc
    34501 gttttgttta ctggctgctc caaaaaggat gaggacctta caactctgcc cgaaactgag
    34561 ccgcaggcgg accccgaaaa aataaacccc tttacgggct tgaaatatga aaagtccaag
    34621 gtcagttcca gacccgtttt cgtgtccata aacaacgccc ccgccgcaag accacagtgg
    34681 ggacttacaa gccccgacat gattatggag tttgaggtcg agggtggcgc aactagaatg
    34741 gtttgggtgt atgccagggc tgaggatgtt cccgaaaaaa tcggctccgt cagaagcgca
    34801 aggcattatt ttgttgagat ggcagagggc tttgacgcca tattcattca ctggggcggc
    34861 agtccttacg cctacgacag aatgaaggag cttaagaccg accacatcga cggaatgtcc
    34921 tactccacaa agtactttgc ccgtgatgag tcaaggaaag tgggacttga gcatactggc
    34981 tacacgacag gctcgaatat cataagcgcc ataaaggatt tgaaggtcag aaccgaaatt
    35041 aaggatacat acaaaaatcc gtttaatttc gctcccgaga attctccaag agagctaagc
    35101 ggtcagagct gcagcgagat taacgccgta ttctcctcaa ttgtaaagta caatttcaag
    35161 tacaaagccg ccgacaaccg ttattacagc tatcttaatc agaacaagac ggttgacgac
    35221 aagggccagc aggtagcggt tgaaaatgtg tttgtcctct ttgccgatgt ggagagcatg
    35281 aacacggcaa aaggtcatgt gacctacgac ctgtccgttg gcgagggcta ttacgcttac
    35341 ggcggaaagg ctgagagaat caagtgggtc aagggctccg acagggatac gctgaaactc
    35401 tatgatttaa acggcgagga gctaattata aacccaggca agagctggat gggatttgtt
    35461 aagaccgcca acgaaaagca gagcacaata gttgcataaa aaaagccggc ataaagccgg
    35521 cttttaattt ttccttattc aagcagctct ttaaggcttt ccatagcctc ctcaatttga
    35581 taaagaatct cctcaagctg ggttacggcg ttttccattt cttcatactc gtcggtatcg
    35641 taaaactcct cgggggtgtc tatataggcg ctctcctcat cgctaaaaac agcttcgata
    35701 atatcgtacg catcctttaa aatcggcagg gcttctttca gctcttcctt ccttttttga
    35761 gtcataagct ttcagctcct tttagctgtt tatttcggca aaagaaaact ctgcggcaaa
    35821 aaccgcagag ttattttatc caatttcttg ttattcgaaa ctttccttat ccgaaaatcc
    35881 aaacaataat cgccatccgg cacatcgaat gttattttca tgattactct cctttctcaa
    35941 ttattgaggc tccgatcacc ggtatcatta ggtcgttcgg cttttccacc gtcccatata
    36001 ctacaaccct atcaccttcc gcaatatctc tttcaatctc gccaacattg aacgctgcct
    36061 ccacggaata cacgccatag ccatcgcctt cctgcgtagt caagtcgaat atgataattt
    36121 ggtcagtctt taactcaatt aaactaacct cgccaactgc ctttacgaat ttcccttccc
    36181 attccccggc gtttatttta acaaaatccg cttcaactgc ttttggccga gggtcttcgc
    36241 ctatcatttg cgtcgggtca accgccggtg tattcatcgc ccaaatcatc gtcgctaatg
    36301 ctacaacaaa gaatactcct gttaaaacgc ctattacctt cttaggtttc ttccttatcg
    36361 cctttattaa cagaattatg ccacatacta taaagccaat tacactcacc acaaacaagc
    36421 ccataaaaac accaccttat tactttatta ccttcttcat tttaccatct tttgtgaaat
    36481 tatgctacaa tcttcattgt tctttagttt tcttctcagg gaactgagaa taattccata
    36541 aaatgcctta gtttttcaat ttcggttcat gttgcccttt tacccaatcg gtttttccat
    36601 atctgtaata accctcataa gtcttactgt tatgcgagca tacgcttatg ctcctgaacc
    36661 acagaattac ctgtcgaagc aaaagcggtc atgggaagca gaccaacaat caatgccaac
    36721 atcagcacac aaacaaacag tattatttgt aaaggggacc gtaggtctgg catgcgcgat
    36781 agtcggcgct ctcaaggtct gctgtgccga atgcgctcca agcatgggga cggaaaatct
    36841 tttcctcagg cacattgtgc atgcatacgg ggattctgag catgcttgcc agagtaatca
    36901 ggtctgcgcc gatatgtccg taggacagag cgccgtggtt tgcgccccag tttgccatta
    36961 ccgagtacac atccctgaat gcgcccttgc ctgtaaggtt cgggacgaac caagttgtgg
    37021 gccatgtggg gtctgtgcgc ttgtcaagag cttcatggac atcgtcgggc agaacaacgg
    37081 tgtagccctc agcaatctgg agtacggggc ccagaccgtc tataaggttt acccttatca
    37141 ttgtcatggg catttctgct cttgttctga agtgggatga gtagccgccg cctctgaagt
    37201 agcctgtgtc agcggggcac cagtcggtga ctgagagcat attcttaatg tcctcgtcgg
    37261 tgacttccca ccacttcttg attacggggt tgccgttctc gtccttgcac atacctgttg
    37321 cgtcaagggc tgcggaacct gagttgataa ggtggataaa gccgtttgcg gctctgcctg
    37381 tgagcttctt gccggttact ctctcaacag cctcggggct ccagtaggtt cttacatcgg
    37441 cgaaaaggct tgcggtgttg gtaaccagct tgccgaagag cattgcgacg ccgttaagac
    37501 cgtcgttctc ggttgcgaag ataaggggct ccttcttgcc gttccagtca aagctggagt
    37561 tgaggatagc ctctgagaag tcgccgttgg ggagatagtc gttccaatga cgctgaccct
    37621 ggaaaccgct gaggatggcg ttcttgccga gagcctcctc atgccagccg gagtcaagcg
    37681 cgccgggcat ctttgcttct tcgccaacgg gaacgatgtt ggcaagcttc gggttgccaa
    37741 gcataatgtc acggaagatg agagtcatct taacaacgaa ctcccactcc ttgtccttct
    37801 gctctcttgt atggggcttg gggttcttgt caaagccctc ggggcagttc tccttaaccc
    37861 atttaagagc cctttcgaac tcctcatggt cgtaaatgcc gaggtcaatg cggcgcttga
    37921 tttcggtcat gtctatccac tcgggacgga tgccgaggta attctggaag aacatcgggt
    37981 ccatggcgga gcctgcgata cccattgaaa cggagccgat tcccgcatat gacttgcctc
    38041 tcatctgacc gacagcgacg gcgcagcggg caaagcggag aagtttttcg gctacatcct
    38101 cggggatgct ggtatcggtg gcgtcctgaa catccttgcc gtagattgcg aaggcgggca
    38161 tacctctctg agcgtgagct gccataactg ccgcgagata aaccgcgccg ggacgctctg
    38221 tgccgttaaa gccccaaact gccttaatgg tcagggggtc aaggtccatg gtttcgctgc
    38281 cgtagcacca gcagcgggta acggacagag tcgcaacaac attctgagtc gcgaaatact
    38341 cggcgcactt ggctgcctca gcgcctccgc ctattgtgca gggggaaatg acgcactgaa
    38401 cgggtgtgcc gtcggaatag tatacattgc tttcgataaa ctcctttgca gccttagcca
    38461 ttgccattgt ggcgtcctca agactctctc tgactccgcc ccagcggcca tctataatag
    38521 gtcttatgcc tattttgggg taactcatat tttgttctcc tttcaattta tctgaatttc
    38581 ccttacagaa attatttacc gacaatgccg cagaatctgt cgtatgccgc gtcaaaagcg
    38641 gcaggattct tcggctcata gacctttatg tcctcgcagc gggagataat ctcccttgct
    38701 ttttcgatgc tctctatgct gccgtcggca aggagctgaa cggcgatatt gccgagaaca
    38761 gccgcttcaa tgggtcccgc gtggacgggt cttgcgcagg agtcggcggt catgcttgaa
    38821 agcaaaccgt ctttgacgcc gccgcccact atgtgtatct tatcataaac ctttttggtg
    38881 catttttcaa ttcccaatag ggtatatttg tatttcatgg caaggctttc ataaatacac
    38941 cttacgattt cgcccttaga ctcaggaaca tactgacccg tgcggcggca gaactccctt
    39001 attctgttcg gcaaatcgcc gggagaaagg aaatccttag cgtcggggtc gataaagcac
    39061 ttaaagggct cgctttggag ggcaaacttt tcaagctcag cgtaggaatg ctcctcaccc
    39121 tctcttatcc actgccttct gctctcctga atgagccaaa gtccgcagat gtttttaagg
    39181 aaggcgattg agcggttata gccgccctcg ttggtgatgt tgagctccat tgcctctttg
    39241 gtaagtatgg gagaatcggt ttcggtgccg agcagggacc atgtgccgct gctgataaag
    39301 gcaaagtctt tattcagcga cggaacggcg gtgacggcgc tttgggtgtc gtgacccgca
    39361 acggcaatga ctctgactct gtcaatgcca agctcctcgg caatatccgc tctgagcctg
    39421 ccgatttccg ttcccgtttt tataatgggg gcaaaaattt ccctcgggaa gcccattctg
    39481 tcgattaggc tgtaagacca ctcgcctgtt ttaaggtcca caagctggga ggtggtggcg
    39541 atggagtact cggagaaaat ctcgcccgtc agcatatagc tcataagatc gggcataaaa
    39601 aggagctttt ttgccctctc caagatatgg gggcgtttga gttttaggga caaaagctga
    39661 tagagggtgt tgaactgtat gaactgtatg cccgtgaggg aataaagctc ctcgcgggat
    39721 atgattttgt ccgcctcctc tatcatgccg ttgtttctct cgtccctgta atgaaccggg
    39781 ttttcaagga gagctccgtc gcagcctaaa agcccgaaat caacacccca ggtgtcaatt
    39841 cctatgctgt caaagccgcc taaatgcttt gcctttaaaa tgccctgctt tatctcgtgg
    39901 aaaagcctta aaatatccca gtaaagggta tctcccacct tgacgggctc gttgaggaat
    39961 ctgtgaatct cgtttaaact tattctctct ccgtcaaagg tgccgagaat agctctgccg
    40021 cttgaggctc cgaagtcaaa agccaaaact cttttttcgc tcatttcatg gcctcctaag
    40081 ctatcttaca accacgcctt ttttgatgat tacattggcg taaagggcgg tttcgcttgt
    40141 ggcgacaatg gcgtaagcct gccttgccct ttcgtaaaac gcaaacctgt cgatttcgct
    40201 gattttaaag tcgcccgatt tcgcgactat ttccgcatag gtttttttga cattggggtc
    40261 aacagtatcc cccggcatgg gagccataag gattacgggg ctttctaccg cggtgtcaag
    40321 ggggaacagt tggagaatcg cgtcaagcag aggcggtacg gtgtgaccgt cacagcggac
    40381 aagacgcttt gcgttggagg cggcggggaa attgccgtcg ccgataacca gctcgtcgcc
    40441 gtgtcccatt tcgtgcagaa tctttaaaag ctcgggggaa ataatgtccg gaataccttt
    40501 aagcataatt tttcaccttt cagattattc tctaaagcgg acttgccgcc gattgcgttt
    40561 cttactcgtc gccgttgttg agagccgcta tcctctcggc tctcattcca gccagctctt
    40621 gaagcattct ctctttgaat gagccgtgga cattgaagaa cctgagagct ccggcctcaa
    40681 ggaatctgct gatagctgga acaagagaga agacggtcca aatggctctg agcattctgg
    40741 ggtcggtctg gggcacaacc ttgccagcgg cgttgataag gggcttatac ttaatagcct
    40801 tgattaccgc gcctgtaatg agcttaccga gtgtgtttgt ggcaagaccc atgtatccca
    40861 tcgcggcgga gagcataccc tcgtttctaa agcctgtttt ccactccaca tagtcgtaca
    40921 tatcgccctg cagagcggtg ttgcaatatc tttgcacgct gttgaaggct cccgcgaagg
    40981 taagaccgag aaccacccac gccatattta aaaacgagct gcttgtgggt ttgtagccta
    41041 caagatacaa aatcagatat gacattccgc tgaatgtaaa gcagaatgtg ccgagatttc
    41101 taagacccat tttttcggta agttttgtgg cgaagggcag cacaaaatag ctcggtatgc
    41161 ccgtaaagag acctgcgaag aactggtttg acagcttgcc cgtgcagtta agccagaaat
    41221 agctttcaac attgacgccg attcctttgc ccactccgaa aaagcccttt ataagcagca
    41281 cccagacggg tctgcagttg gcgaccattt ttaccgagtc gaaaacggaa tactgctgca
    41341 tggtctgtct tgaggcaagg gggattctct ccctcaaagt caaaaagccg taaattctga
    41401 aaacggcgac aatcattatg aagaacatag ctccgcctat ataaatatac cttgttggta
    41461 tattgccggg caggtagtcg ataaagaacg ggaaaagcga tggaatccaa acgccgaaaa
    41521 ggtccacata agtatcggcg gctatgagtc tgtccctctc atccacattg ggggtaaggt
    41581 tataaagaat agccgcgtaa gcgggaccgt aaaaggagtt tgctattgag cctaaatact
    41641 ggagtatcaa gagaatgaaa aagagcgcgg tttgtgatag gttcgtcggc aaaagccacg
    41701 gcatcgccgc ggacagcagc cagagaggca gagtcgcaac aagataaggg cgaactctgc
    41761 cccagcgggt tcttgttctg tcgatgatgg gacccgaaat cgcgttatca agagcgtcgg
    41821 caatgccgca aagcgagtcc accagcgcaa gggtcgtggg cgaggctctt aaaaaggttg
    41881 tattgaagaa gtatgtattc tgggagctga acgactccca atttttgtct ccgatgcctg
    41941 aaattatgta ggcggccata gccttggggg aaaggtattc cctcattttc ttttcaggct
    42001 cggcagaagg agctgatata atctccgcag tgttgagtct ggcgttttct tctgccgatg
    42061 tcataacaaa cctccgaatt gagatttttg caaagcgact aaaaatgccc caaatctact
    42121 tcaattttta tactatttca acggtaataa tagcatatta aaattgtcaa tgtcaatcag
    42181 agcgcccttt tatgatttgg gtggaataaa aaaaaccgcc ctcgtaaatc gaaggcagtt
    42241 ttgtgttttt atcagcggtg ctttatttca agatttaaga ggaatttctt catatttaat
    42301 cccccgccgt agcctcccgg tgttccgtca gcggctatta ctctgtggca gggaataatg
    42361 atgggcagtc tgtttttgcc gtttgcgcag cctaccgccc gtgaaccgct gggattgccg
    42421 actcttacgg ctatgtcctt gtagcttctt gtctctccgt agggaatttc cataagggcg
    42481 ttccagactt ttgtctgaaa gggcggtccg tcaatgcgta aaggcaaatc aaattttttt
    42541 ctttctccat tgaaatattc gcttagctgt ttgtgagctt cttttatgag aggagtttct
    42601 ttatatgtat aatcaaaatc aggattctcc ccgggataca gaatgtccgt aagctcaccg
    42661 ttttcttcag ctattcctat tttcccgatt gcggtatcgc aaaaccacaa gcttttcaaa
    42721 acattccccc gctttcaaat caatatttat atatcgtttg cgatgaggtc atcatatgat
    42781 tctcttttta tgatgagcct cggttttccg tcagtaatca tgacgacagc gggtctcggc
    42841 actctgttgt agtttgacgc catggaatag ttgtaggcgc ccgttgagag cacggcgata
    42901 atgtcgccct cctgtgtctt ttgaatcatg gcgtcctttt ggataaggtc gccgctctcg
    42961 cagcacctgc ccgcgattgt agccatgtaa tccctcggct cgttcatttt gttcgcgcag
    43021 tggatatcgt actttgagcc gtagaggatg tatctcgggt tgtcggtcat gccgccgtcg
    43081 atggacacat agttcctgat gttcggtatt tcttttacac tgccgacggt gtagagtgtg
    43141 actgcctccg ctcccactat tgagcggccg ggttctatca ttacaaaggg tgttttgatg
    43201 ccgtattttt ctgcggtcgc tttaaggctg agagctacct ttttcatata ttcgctgaag
    43261 ctctctggat tgtcgtcgcc ggtgtatttg atgccgaagc cgccgcccag attcagctcg
    43321 gtaagctccg tgcccgtttc tttcttaatt tccgcgatga aggctatcat tacctccgcg
    43381 gcgagaatga aggggtctat gtcgaaaatc tgcgagccga tatggcagtg aagtcccata
    43441 agttctatgt tctcttccgc caaagcgagt ttgacagcct ccatagcctc gcccgtttca
    43501 agggcaaagc cgaacttgga gtcgatttga cccgtcttta taaactcatg ggtatgggcg
    43561 tcaatgccgg gcttgattct taaaagaact tttgcggtct tgttcttttc ggcagccgct
    43621 cttccaatct ttcttagctc ctcgatattg tccacgatga ttctgcccac gcccgcgtcg
    43681 agagcctcgc aaatctcgct gtctgtcttg ttgttgccgt ggaagcagat tttctcgggc
    43741 gggaaatctg cctttaaagc ggtgtaaagc tcgccgcctg aaaccacatc cagacccagc
    43801 ccctcggatt tgcatattct gcacatttca aggcaggaga acgccttgct tgcgtaataa
    43861 acaagtcctt ttccctcgta atgctcgttt attgaggaaa caaactccct gcagtttttt
    43921 cttatggtct cctcgttgta gacaataaga ggcgtgccga actgatttgc cagctccacc
    43981 gtatcgcagc cgccgattgt gaggtgaccc ttttcattta cattcaaaca ctctgataca
    44041 aacatccgtc tctttccttc cgcattagcc tattacttct gctccaaaac atcgtctata
    44101 taagacctta attccttaag tcccacaccc gtctgagcag aaaactcgat tacttcaact
    44161 tccctgtcgc cgaggaacgc aagttcttca ttcaaagcct cgaggcgttt ctgcctctct
    44221 gtgggcttta gtttgtcgct cttggtcagc actattacaa acgggaggtc ttttgctatc
    44281 ataaagttta tcatgtcaat gtcgtcgctt gtggggctgt ggcgcatatc gataagctgg
    44341 acaatgagtt ttatattcct gcccgaattg aaatagccct ccataagctc gccgaaacgg
    44401 cgtatctccg ccgcggagcg tttcgcgtag ccgtagccgg gaaggtccac gaatctgacg
    44461 cccccgagcc tgtaaaagtt tatggtgatg gttttgccgg gaacgctgct gactttagcg
    44521 agctgctttc tgttaaagag cttatttatc attgaggact tgcccacatt ggagcgtccc
    44581 gcaaaaacta tttcgggtat attggatttt ggaagctggg aggatagtcc gaatgatgct
    44641 tcaaactcta caatgttaaa gttcatttgg tacctcctga aaaataaact aattaatatt
    44701 ataccattgc cgactgccct ttagcaacag tcagcaatgg taaaaatgaa tttttattca
    44761 gaaatactgt atatcaggtt gtccagacct gcgcgccgtc gctgactttg ggaggggtga
    44821 ggatatgtat ctctcctttt gccgactccg gcttttcgtt gagaatagcg cagtcaaaga
    44881 cctcgtcaag ggtgcgtacg ggcacgaatt tgagcgcctt ttccacagcc ttgtcaatct
    44941 ctttaaggtc ggagagattg ccgtaaggaa tcagaaccgt cgtcataccc gtgcggtatg
    45001 ccgccatggt cttttcttta agaccgccta tgggcagtac cctgccgcgt agtgtaatct
    45061 cgcccgtcat tgcaaccttg ccgttgacct ttttgccggt taaagccgag taaagggctg
    45121 tggctatggt aacgcccgct gaggggccgt ccttcgggac cgctccctcg ggaacatgga
    45181 tgtgtatatc cttattctta taaaactcgc tgtctatgcc aagctgactt gcctttgagc
    45241 gcagatagct caccgcggtc cttgcggatt ccttcatgac atcgcccagc tgacccgtaa
    45301 gctcgatttt gcccgtgcct tccatgatgg ctacctcgat ttgcagcatc tcgccgccga
    45361 cggaggtcca agcaagtccg tttgcgatgc cagtctggtc ggcatagagg gaggggtcct
    45421 ccttatactt tacgggacct aagaactcgg agaggttctt gtccgttatt acaaggggct
    45481 ccttaatgcc gtctgcgagt cttacggcct ccttgcggca gagcttggag ataagctgct
    45541 caaggcgtct gacgcccgcc tctctggtgt agccctcaat aatgagcctt aaagccttgt
    45601 ccgatatgcg aatctgcttt ctggaaagtc cgtgctgctt aatttgtttg ggcaccagat
    45661 ggcgttttgc tatatggaat ttttcctccg ccgtgtagct tgaaagctca attatctcca
    45721 ttctgtccct taacgggccg gggattgcgg aggtatcgtt cgcggttgtg atgaaaagga
    45781 cctgacttaa atcaaagggg atttcaagat agtggtccac aaaggcaaag ttctgctcgg
    45841 agtcaagcac ttccagaagc gcggacgatg ggtcgcccct gaaatctccg ctcagtttat
    45901 caatctcgtc aaggagaata agcgggttcg atgtgcccgc ctgagttatg gcggtaataa
    45961 tcctgccggg gattgcgcca acataggttc tgcggtgtcc tcttatttcc gcctcgtcac
    46021 gcacaccgcc caaagacagc cttgcgtatt tgcggccgag cgcagtggct attgactttg
    46081 ctattgaggt tttacccacg ccgggaggtc ccgcaagaca gataatttga cccttgagct
    46141 cctttgagag gaaccttgcc gcaagcattt cggtgattct ctctttaacc ttttccagtc
    46201 cgtagtgctc cttttccaga actctgcgga cggaggcaag ggagttcttg tccttggaat
    46261 agacgcccca gggcagagca aggcagcggt cgagataggt tcttataacc gcgctgtcgg
    46321 gagaagcggg ggacattctg cggagtctgc ccgcctcctc aaggagcttt ttctttgact
    46381 cctcgggcat cttgctttct ttaatctttg ctatgtaatt atccgcgtcg gacgcgggag
    46441 agtcgccctc ctgcagctcc tcgcttatga ctttaagctg ctctctgagg tagtactccc
    46501 tttgattcag gtcaatctgc tcctgaacac ggtcctgaat atcctcttcc agccttaata
    46561 tatcaatttc cttagtgagg ataacgcaga gctgctcgat tctccttaaa ggatttttct
    46621 gagagagcag gttctgcttg tcctgcatgg gcagcgggat agcggcggct ataaggtccg
    46681 agagtatgcc ggggcgctcc tcctgctcca cgcttgcgat aacatcggga gcgatttttg
    46741 gggaaagctc ggaatactcc tcaaacaaat tcttagcgcg gcggaccaaa gccagtgcca
    46801 gttctttgtc cacgctctta atgggcgggt cgtacttagg tttaattgaa gcaatcatat
    46861 agggtttttc ttgaactagg ctgtcgattg aggctctgtg cttgccctcg actattaccc
    46921 tgtatgtact gccctcatgt atgcggaaaa cctgccttat ctccgatatt actcccatcg
    46981 gataaaggcc gttaaggtcc ggttcttctt cgttaatatc cctttgggta actaagaaca
    47041 aatcctgatt gccctcaagg gctgctctca aggctgccgt ggacgccttg cgccccacat
    47101 cgaaatgtag cgtcatgccg gggaaaatca ccaggcctct taaggccacc agcggcattt
    47161 gtaaagactt tgcaccttcc atcatattat caactccgta cggtccaaac actgctccgc
    47221 cttggggcag acgcaaacca acaaatctgc ggtgccttcg accaaatcta tgtaaattac
    47281 aggaaatcag caatattaca tcagtatcat tatatagtta aagcccctat ttttcaatac
    47341 ctgcggagct tttttggaga tagatactat aatatctaga atttttccat tttgttgtct
    47401 atttccgtga aaatggaaaa atttttcttt tttgttgcaa agcgacaaca ttttagagtg
    47461 tcatcaggct ggcgcattgt cgcattaaag aacaaaacac aagagagcaa aaaccctcct
    47521 gtgttttttg aagtttacga aatttgttta gtagattcag tgtgtcatcc gtgtaacagc
    47581 tcttcgaaaa atgtcggagc gtcggcttta ttaataagct ccatgttttt gttggtaaga
    47641 cacattcttt ctatgtattt aaatgtttca aaagacagtc ccatatccac ggcgatgatt
    47701 ttgcagtggg tttcgcccag gaggtttctt ttctcaatcg ccgcgcagat ttttgttatg
    47761 gattcttctt cattgtcaag cggcagaaca agatagtact ccttgttctt tttgggactt
    47821 aagagcttca gcgtacccgc atagataatg gccaaaagac cgattaaagc gaaaatcaag
    47881 gttaaaccgg cgaccagact ttcgagcata aaaaaatcac cccataacat attatggggt
    47941 ttgtgattat atgtgcgagc aaatctgtaa gccgcgttct gtcatttaag gcaattatct
    48001 atcttggccc gatgttgcca tcgggctcac gccacctgtt ggggatatgg ccgagcaggc
    48061 cccccggtcg gtgttgcttc ggatagggtt tgcagggccg tgcggtctcc cgcacgccgg
    48121 tgagctctta cctcgccttt ccacccttac cgttcaaaat gaacggcggt atatctctgt
    48181 tgcactttcc ctaaggtcac cctcggcggc cgttagccgc tatcctgctc tgtgaagccc
    48241 ggactttcct catgcagttt cctgcacgca attgcccaat ttgctcgcac ttcggaaaat
    48301 tatattatct caaaaccgaa ataaagtcaa tccgcgggcg tcatttggct caatttacat
    48361 gatattttga aaaaatatcc aatattataa tacgccgaaa aagtgggcaa tgttaaggtc
    48421 ataaggctcg tcggtgccgt gggagcccct gccgttgggc tcgttatgga ctccatggtc
    48481 ggttgagaat gtgatgaggg ttttgccgtc tctttcggtc tcttttatgg cgtttgccag
    48541 ctcgtcaaaa attcttattt gattttttaa ggcgtttata gcctcggggg agagcactcc
    48601 cctcgcgtgc attacatcgt cgtattcctg agtgtagacg ctgaggagat tgtatttgtt
    48661 ttcttttatg agccttatcg ccgtttcctt taccttttcg tcgtcttctt ctataaagta
    48721 atccatatcc ctttcgagga aaatctttga catgctgctc tctctgacag cgactatcgc
    48781 ggcttttttg cccgctccga taaaggcgtc gtaaacagaa tctatcttaa ttacaggttt
    48841 tgtgtagaac tgaatcccgt gaacttcggg gagagcgccc gtatacatgg tgccgaagca
    48901 aacgggagtt accgacggga aaacggtttt gaagggcagg catacggggg catggcgcag
    48961 aaccttttcg aaaagctcaa aattctgctg ataaagccac attgccacag cgtcgggatt
    49021 atgtataaca attctgtcaa agggagcgtc attaaaaagt tttaaaatac tttcgacggg
    49081 ctcgtcggct ttttcgggtt tttcaattcc cgcaagagcg caaattgttg aggcaaaacg
    49141 ggttaaacta atactgttga atctatagtc cataacgcat ctccagactt ttaaaagatt
    49201 tgttccagta tagcacaaaa gatttgtcaa tgtgtgaatt tttatcaagc gggcggcttt
    49261 tcactttaat ttaattgact tgaacccttt aaaaagtata atgagtttaa aggttaaatc
    49321 ggctgcgacg cgcgcccgaa attcaggagg tcagtatgga caaaatatta tctgcgtcat
    49381 ttttaaaggc tcccaaatac aaggaggggg cggcttttgt tttcagcaaa gagttcgagt
    49441 cagacaagcc aattaaatcc gcaacgcttc aaatcagcag ccttggcgtt tatgtggcat
    49501 acctcaacgg gcaaagggtg ggagacttta tacttgcccc cggctggaca gtgtatgaaa
    49561 agcgccttca gtatcagaca tacgatgtaa ccgatttaat taaagacggc aaaaacataa
    49621 taaccgcaga agtgggcagg ggctggcgtt ttcacaaaag accccagcat gcaaccaaac
    49681 acatcaaggc ggacgaaccc gcgttaattg cctccctttg ccttacatac aatgacggct
    49741 ccgaggcaat aataaatacg gacagcactt ggaaatcctc agagagcaaa accgtctaca
    49801 acgatatgta cggcggcgaa acctacgacg acaatttcgt atctgaggaa aaaagcgctg
    49861 tcgccttccc ccatccgaag gaaattctga ttccgcagga aggagagaag gtggcggaga
    49921 aggaaagact caagccccaa aggctcatta ccactcccga atgcgatacg gttattgact
    49981 tcggtcagaa cctgacaggc tatgtggagt ttaaggctaa gggcaataaa aacgatgagc
    50041 ttgtaatcac ccattttgag gtccttgaca agaacggcaa tgtatatacg gaaaacctta
    50101 gaaaagcaaa gcagcaggta aggtatatct gctcgggcga ggagaagctt tataagcctt
    50161 cctttacctt ctacggcttc agattcataa agctggaggg ctttaaggat gtaaaccccg
    50221 acgactttac agccatagcc gtatactcgg atatgaagag aatcggtcat tttgaaagct
    50281 cggatccgct tctcaacagg ctgtacgaaa acatcatctg gggtcagagg ggcaacttcc
    50341 tggatgttcc caccgactgc ccgcagaggg atgagcgact cggctggacg ggcgacgctc
    50401 aggtatttat aagggtcgcg tccatgaact atgatgtcag caccttcttc agaaagtggc
    50461 tcaatgacat ggcagccgag cagggcgagg acggcaggat tacccatgtt atccccgcga
    50521 cctttgacgg cggcggctct gcggcttggg gcgacgcggc tacaatctgc ccgtggcagc
    50581 tttacctctc atacggcaac aaagaggacc ttgagcacaa cttcccgatg atgaagaagt
    50641 gggtggatta tatcagaaag agcgccgctc acaagtccct catacagtgc ttcaaaggct
    50701 ccagaaatcc ctatctttgg aacacgggca aacacttcgg cgactggctc gcccttgaca
    50761 ttgagggtga tgacgaggac gcaaggggcg ctacggacca cgactacatc gcaacagcct
    50821 tctttgcgta ctcaaccgaa ctgcttatca aggcaggcga ggttctgggc aaggatatga
    50881 gcgagtacaa ggctctgcac gagaacatcg taaaggcata cagagaagaa tatgtaaacc
    50941 ccgacggcac aatcaaaata aacacccaga cagcctgcgt actggctctt aaattcggtc
    51001 ttacgcctga cagagaggca accgcaaacc agcttgtgtc ccttataaga aaatacggtc
    51061 accttacaac aggctttgtt ggaacgcctt atctccttca tgtgcttaca gacatcggcg
    51121 aggtagagct ggcatacgac ctgcttttac gcaaagagta tccgtcatgg ctctatcccg
    51181 taactcaggg agccaccacc atgtgggagc gctggaacgg tcaaaggccc gacggcagct
    51241 tcgccactcc cggcatgaat tcctttaacc actacgcata cggcgctgtc ggcgactggc
    51301 tgtactcaac cgcagcgggc atcacaatga gggacggcac cgtcgcatac gaggatttaa
    51361 tctttaaacc ccagaccgac aagagactga cattcgttaa ggcatcgata gaaaccgccc
    51421 gcggcaccgt cgcttccgaa tggagaaggg aaaccgacaa gacggtttat accttcactg
    51481 tacccgcagg ctcaaaggcg gaggcggttc ttgagggcaa gacatataag ctgaacgaag
    51541 gcgtaaacac aatcacactt taatacttaa acggttcttc cctgcagatt ttttctgcag
    51601 ggaagaacag cgaaagacaa atcccgattt atttttaaaa cagcaaaggc gttaaatccg
    51661 cttttttgaa tataaaatcg ccaatctctc aataattact ttgcaaacgc tttacaactt
    51721 catcgcatta taggctttaa gtcattaatc atcaaggttt tgcggaaatt ttcgagaaca
    51781 ttaaaattgc cgtcaagccg attttgttaa aaaaacaaat aattcgtttt ttattgacaa
    51841 attgtacaaa aaacagtatt tagttgtttt ttacagaaaa agactttaaa tttacaatat
    51901 ctgatgatat aatatccggc ggacacaata ttttctattt tagcaatgca cttaaagtcg
    51961 gttttatata tttctttcct ataagcttat attaaaaaaa gggaagtgcc gtatttggaa
    52021 aagttcgtaa taaacggcgg gaaaaggctc tgcggcgagg ttgaaatcag cggagcgaag
    52081 aacgccgccg ttgccatcat ctccgcaacg gttcttgcag atggtgtttg caggcttgaa
    52141 aacattccta atgtccgtga tgtccgcatt cttatgcaga tattggacag catgggcgca
    52201 aaggttcgca atataaacaa caacacttgg gagattgact cctccactat caataccacc
    52261 gtcgtttcaa atgaaatgac gaggcattta agggcatctt attacttcct cggcgctctg
    52321 cttggcagat acaagcaggc taaagtgggt atgcccggcg gctgcaattt cggagtccgc
    52381 cccatcgacc agcacatcaa gggctttgag gctctgggcg ccaccgttac cacaggcaac
    52441 gccatggtgg aggtaaaggc aagggagctc atcggaaact ccgtttactt cgatatggtt
    52501 tccgtcggag ctaccgttaa tgtaatgctt gccgccgtaa aggcaaaggg ccttacggtt
    52561 atcgaaaacg ccgcaaagga gccccatatt gttgaccttg ccaacttcct taacctcatg
    52621 ggcgcggatg taaggggcgc gggtacggat attattaaaa tccgcggcgt ggagaagctt
    52681 cgcggctgtc agtactccat aatccccgac cagattgagg ctggcaccta catggttgcc
    52741 gccgctgcca caaggggtaa tgtgtttata aagaatgtta tccccaaaca ccttgattcc
    52801 atttccgcaa agctgattga atgcggcgcg gagattgaag aattcgacga ctgcataaga
    52861 gtttcaagca acggcagact aaaccgctgc catgtaaaga ccatgcccca ccccggtttc
    52921 cccacagata tgcagcccca gatggcggtt cttctctccg tagcagaggg cacaagcatt
    52981 atctctgaga gcgtatggga caaccgtttc agatatgtgg accagcttat cagaatgggc
    53041 gcggttattc aggttgaagg taaggctgct attattgaag gcgtgccctt ccttaaagga
    53101 gcgcccataa gggctgacga tttaagagcg ggcgccgcga tggttatagc gggccttata
    53161 gcccagggtc agaccaccat tgaggacatt caccacatag acagaggcta tgagaatgtg
    53221 ctcggcaaac tgcaggctct cggcgccgac attaagaggg ttgagttcaa cacccccgtc
    53281 acaaagcccg taagcattgt aggctaattt aacagattaa aagaaaagcc ctgagggctt
    53341 tttctttatt acaccaaaac ggagaacaac atggcgagat tcacaacact cttttcttca
    53401 agctcgggga attccaccta tttgggaacc tcctcgggcg gtattcttgt tgacgcgggc
    53461 aaaaacgcaa agcagcttga ggctgctctt tccgcgataa atgtaagccc gactgaaatc
    53521 aagggtattt tcatcaccca tgagcatacc gaccatataa gcgctgtgcg ggttttcgcc
    53581 tcccgctacg gcattgatat ttacgcatcc aaaggcactc ttgcggaact tagaatgcag
    53641 ggacatttaa acggcaaatt caacgccatt ccaatagacg aaaagggcgc cgtggtggcg
    53701 ggaatacata tagcgccgtt cagaacctcc cacgacagca gggagagcac cggctacacc
    53761 gccgtatttg aggacggcag aagaatagga atcgccaccg acacgggagt catgactcag
    53821 gagattctca ccgccctttc gggctgcgac gccgtgctgc ttgagtcaaa ccacgatgta
    53881 tggatgcttg aaaacggctc ttacccctat tatttaaaga gaagaatccg ctccaatttg
    53941 ggacaccttt caaacgacga ctgcgcctct gctctgtgca gacttataca aacgggcaca
    54001 acaagaataa ttctgggcca tttgagcaag gagaacaaca gggcagaaat cgcaaggcag
    54061 acctcagttt cggctctgag caccatcgga gctgtggaaa acagggatta tatcctgagg
    54121 gtcgcttccc acgacagacc cgacaaaatg ctgatatttt aagctgatgg aatagcctcg
    54181 cattcgggag ggcaaaactt gataaaggta aacattattt gcgtagggag attaaaggac
    54241 gcattttttg aagaagccgc aaaagaatat ataaagatgc tgggctcctt ttgccaaatt
    54301 aagataaccg agataaacgc agcaaatctc ccccaggagc catcaagagc tgagattgaa
    54361 aaggcgctga tggcggaagc aaaggagata ctgaggaaaa tccccgaaag gtcaaagaca
    54421 atagcccttt gcattgaggg gaaacagctt tcatccgagg aatttgccga tttgcttgaa
    54481 ggctgcaaaa gctcgggcgc gggtgaaatc tcttttataa taggcggctc atacggtctt
    54541 gcagacgata ttaagaagcg ggcggacttt aggctctcct tttcaaaaat gaccttccct
    54601 caccgtcttt tcagggttat gctccttgag cagatataca gagctttttc catttcggca
    54661 ggcggcaaat atcacaaata gtataaactt gcatttttgg acaggttttt tactcgcaaa
    54721 tattgaaatg gctgttaaat ctgctgtaaa ataacttcaa aattgtatat attttaggtt
    54781 taattcaaag aaatgcggat attctaaaat aatatggaaa tataggaaaa tatctgttat
    54841 aatgtctttc ggtcaaccct gccctttttg gggcaagcac gaatgtgaaa tatttaaatt
    54901 taaatatgga ggaattattg aatgagtcac aagtatgttt atttgttctc agaaggcgac
    54961 ggaagcatgc gcgagcttct gggcggcaaa ggcgcaaacc ttgctgaaat gactaagctc
    55021 ggtatgcctg ttccccaggg ctttaccgtt accacagagg catgcacccg ttactatgaa
    55081 gacggaaagg taatagctca ggaaatacag gaccagattt acgagtacct tgccaagatt
    55141 gaagaaatca acggcaagaa gttcggtgac cccgaaaagc ccctcctggt atccgttcgt
    55201 tccggcgctc gtgcatcaat gcccggcatg atggacacaa tcctcaacct cggcattaac
    55261 gacgaagttg ccgaaggtct tatcaagggc aatccggacc cgtcattcgc acgctttgtt
    55321 tatgactcct atcgccgctt tatccagatg ttctccgatg tcgttatgga agttccgaag
    55381 cacaacttcg aggtcatcat cgacgagctt aaggctaaga agggcgttaa ggacgacatc
    55441 gagcttgatg ttgaagacat gaaggagctt gtaaggctct ttaaggcata ttataaggag
    55501 cagaagggcg aggacttccc ctccgacccg agagctcagc tcatggctgc cgttcaggct
    55561 gttttccgtt catgggacaa cccccgcgca aatgtatatc gccgcatgca cgacatcccc
    55621 tattcatggg gcaccgcagt taatgttcag cccatggtat tcggcaacct taactccaac
    55681 tccggtacag gcgttgcgtt ctcccgcgac cccgcaacag gcgagaatgt tctcttcggc
    55741 gagtacctca tcaacgctca gggcgaagat gtcgttgcag gtatccgcac acccagccat
    55801 atttccaagc tcaaggaaga aatgcctgag gtttacgagc agtttgttac aatagccaag
    55861 aagcttgaga atcactataa agatatgcag gatatggagt ttaccattga aaacggcaag
    55921 ctctatatgc tccagacaag aaacggcaag cgtacagcta cagcagctct caagatagct
    55981 gttgaccttg ttgacgaggg tctcatcgac gaggaaaccg cagttctgag agttgacccc
    56041 aagcagcttg acgcgcttct ccatccccag tttgacgccg ccgctcttaa ggccgctaag
    56101 gttatcggca caggtcttgc ggcatccccc ggcgcagcaa ccggtaaggt tgtattcaca
    56161 gccgacagag ctaaagagct cgctgagaag ggcgaaaagg ttatccttgt tcgtcttgaa
    56221 acatcacccg aggacatcga gggtatggca gcagctcagg gcatcctcac cgtcagaggc
    56281 ggcatgacct cccacgcagc agttgtcgca agaggcatgg gaacctgctg cgtatccggc
    56341 tgcggcgaaa tcaagatgca cgcagaggaa ggctacttcg aactcggcgg caagaagtac
    56401 tatgaaggcg actatatctc ccttgacggt tcaaccggca agatttacga cggcgaaatc
    56461 aagacagttg aggcttcaat ctccggcgac ttcggcagat tcatggcttg ggctgacaag
    56521 tatcgcacac tgaaggtaag aacaaacgcc gacactccga cggacgctgc tacagcagtt
    56581 cacttcggcg ctgagggcat tggtctctgc cgtacagagc acatgttctt tgagggcgac
    56641 agaatcaagg ctatccgcga gatgatcgtc tctaagacag cagaggctcg cgctaaggct
    56701 cttgagaaga ttctgccctt acagcagaag gactttgagg agatgtacag ggttcttgag
    56761 ggcagaccca tgacaatccg cttccttgac ccgccgctcc acgagttcct gccccagaag
    56821 gaaaacgaga ttaaggagct tgcagacgac cttggcatct ctgttgatga gctcaaggct
    56881 gttatcgaca gcctgcacga ggctaacccg atgatgggtc acagaggctg ccgtcttgca
    56941 gtaacctatc ccgaaatcgc aagaatgcag accacagcag ttatctccgc cgctatcaat
    57001 gtaaacaaag agcatcctga gtacaacatc gttcccgaga ttatgattcc tctggtaggc
    57061 gaggttaagg agctcaagtt cgttaaggat gtagttgttg agacagctga caagctcatc
    57121 gccgaggcag gcgtaaagct cgagtacaag gtcggcacaa tgattgagat tccgagagca
    57181 gctctcacag cagacgaaat cgcaaaagag gctgagttct tctcattcgg cacaaacgac
    57241 ctgactcaga tgaccttcgg tttcagccgt gacgacgccg gcaagttcct tgacgcttac
    57301 tatgacacaa agatttacga gtttgacccg ttcgcaaagc tcgaccagac aggcgttggc
    57361 aagctggttg aaatggctgt taagctcggc aaggctacaa ggcccgacat taagctcggc
    57421 atctgcggcg agcacggcgg cgacccgtca tccgttgagt tctgccacaa tgtaggactt
    57481 gactatgtat cctgctcacc ctacagagta ccgcttgcac gccttgcagc agctcaggca
    57541 gctatcaaag ctaagaaggc ataaaaaagc tataaaaata aggggacagt tacctgtccc
    57601 ctttattttt ttatctgtga ttttctatgt atctgattag ggctcttgcg aaggctttgc
    57661 cgagttctat gagcccttcc tgacttactt tattttcttc agtgccgaaa taggctgttt
    57721 cgagggtaaa ggaaagctcg gtggtcggaa gggatgagaa aaactttgtg gaggtcgggt
    57781 tagttaaggt attccatgag gtattgggca taatgtcgct gccgacaaag tacctgaaac
    57841 tcagattcgc cgactcctca tggaaaagag aggcgaactc atcctgccct tttgtgttta
    57901 agaggttcct gacaaggaat accttgtcat ctccgccgcc tatatggtat ggcgaatgaa
    57961 agtcaaattg gaactttaca ttgtgctctt ttgcgtatgc cataagctct ctgaccgagg
    58021 gatagacggg gttttctata taatccctgt tgtggtcgtg gggatggcgg ttcttgccct
    58081 ggtcgccgaa tatcacgccg tcaatgtcca taaagggcac ggcggcgact ctgtatccgt
    58141 cggggagatt ttttaataga tgctccagaa ccccctccag cacataggtg ccggttgatt
    58201 cgcagcagtg gtgccttgaa gtgagcacta tggttttatc cccgccgccg aactccagta
    58261 ttttgacttc gttccccttg tcggttaccg caaaggtgag ggcgttgaga ttgtgcttct
    58321 tcaaaaactc ctcaaagcgt tcggggctgt atatcatatc gtgggcgaaa tatgtggggc
    58381 tgtcgtcgtc aaaagtgtag gtaaagctga atctgttgtc gccaagggtc tcctcccctg
    58441 cccagttcca agttttaagg tctttgctga cggcggggcc gaaataaccc agtctgtccc
    58501 tgccgaaatc gaaggttatt gtcctgccgc ttgcgccttt tacgcaaaac gcccagtaga
    58561 accagtcggt ggtggtatcc cgaaggtcgc actccacctt aacataatca ccctcgacgc
    58621 ttaacacttt aatatttccg cctgtgaaat cggtagtaat ccgcataaaa atctccttac
    58681 aatttatcgc cccgctccca aggagcgagg cgttttttta atagcccttt ttattctgcg
    58741 cttttgcctt tttcctgagg aagaatatcg gtatgactat gagtaccgag aagattgccg
    58801 cggcgaggaa aatctccggc acggggacat agacagcctc tccgaagtcg ttgtagtaag
    58861 ttccaagctg atggagagga gcgaaacgct caatgaccgc gttgccaagc ggcggaccta
    58921 ttaccatggg cagaagcacc atgaaaatca tacgcacgcc ctggaacaag cctgttttat
    58981 cctccggcgt aaagtccctt acagaggcgt tgaggataat cataagaagt ccgtagcccg
    59041 caaagaccat tacgacggtt gccgcgaacg cgcccagctt tttagcctga gatgtggcaa
    59101 taagtcctat tataaacagc gcgattgacg ggtagatgaa tttgtccttg ccgaatttgt
    59161 ctaccatcag accgaggcag taaaccacgg ctataaagag cacgatggcg acaacggcga
    59221 tgccgataac ctttggggta atattcaggt tttcaaagtc aaagcccatg tagtgctgca
    59281 gataaataaa gagatagggc aggaaaatct gaaccgcgat gttaaagaag gatacggcaa
    59341 ggagggcaag atacaacagg gcgttttcct taattaccga gggtctgaag ccgtaaatca
    59401 aatcggtgat gtatctgccc tgacggcgag gtttaccctc ccttgaatcc tttaatgtaa
    59461 aggcgcctat aaggccgcag agcgaaactc cgatgccgag tcccaggaag caagcgggat
    59521 agcccgccgc ggcgactccc gcgccgaaag ctacggtgac gagaagcgtg gcgagaatcg
    59581 ggaaggacgc gagtatgccc tccgccttgc ctctgttttc gggaacggtg atgtcggtaa
    59641 tccatgcgtt aaaggcggcg tcgttgctgg tggagcctat aaaagtcatg acgcagtcca
    59701 taatgatgac aacggatacc gatgcggcga ggattttcac ttcgtccgca agaccaaaga
    59761 gctttgccac attgtccctt gaaatgaagg caaagaccat gaccgaaaat ccccacagga
    59821 tgtagcctaa agaaaggaaa acctttctgc ggtccagcct gtcgctcaaa ttgcccatta
    59881 ggaaggtggc gataacggcg gttgccgcgg tcaacgctac catggtggaa atgtcctttg
    59941 ttgtgccgcc gatgtagttg aagagaaatg tgttgaagta gatgttttca acagcccagg
    60001 cgacctgacc cataaagccg aaaacaataa tgttaaacca tgttctgcct gaaattcggg
    60061 cgtctttcat agacaaaact cctaactatt tagtttggct tagtaacacg ctaatttaat
    60121 tataccgaag ctgccgtttt ttacaagaag caattgtcat tagccggcaa agaattaaac
    60181 tttgctttca ataaaatcca ggaatttatt ccagtcatag cggctgagga agtgggagcc
    60241 ggggcggagg taatagccta tgctgccgac ttgatatgcg ccctcttcgg gcggaaaggt
    60301 gtcgggggca ataagaccct cttttccgta aagggagtaa acgggggacg cggcggcgca
    60361 tgcgagaaat tcggaagcgg ggtcagccca taaatcctcc gccgcgctgc cgacggaaac
    60421 gaatctgggg gctgagaggg caagaagcaa gtgctggtca aatggcaggg cgctttcgtt
    60481 ctttctgtaa gttttgtagt tttcgcagaa ccagaagggg aagttgcggc agatgtcgtc
    60541 tatctgctcg cctgtcttgc cccttataat ggcggcaccg ctgcagccag agttgttgga
    60601 gtggacaaag cggaacctct catcaaatgc ggcggcgaca agggcggttt tgccaagcct
    60661 tgaatgacct attaccgcta aa
//
LOCUS       DAONVQ010000002        36588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_711_length_36588_cov_5.289191, whole genome shotgun sequence.
ACCESSION   DAONVQ010000002 DAONVQ010000000
VERSION     DAONVQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 36588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36588
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_711_length_36588_cov_5.289191"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..2963)
                     /gene="uvrA"
                     /locus_tag="PLD98_00295"
     CDS             complement(138..2963)
                     /gene="uvrA"
                     /locus_tag="PLD98_00295"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQA04732.1"
                     /translation="MQRNLVIKGAREHNLKNIDVVIPRDKLVVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARMFLGQMEKPDVDYIDGLSPAISIDQKTTSKNPRSTVGTA
                     TEIYDYLRLLYARIGVPHCPECGREIKQQTVDQIVDRILSYPEGTRLQVMAPIIRGKK
                     GEHAKELESAKKSGYVRARIDGSIYDLSESIKLDKNKKHNIEIIVDRLVIKEGIKSRL
                     ADSVELASKLASGTVLIDIVDGEELMFSQNWACPIHGAGIDELTPRLFSFNSPFGACP
                     ECAGLGFQMKVNPDLVIPNKKLSINEGAITASGWNSADPSSIATMYFRGLAKHYGFSL
                     DTPVEKLPKEIIDIILYGTKGEKIHLERTMEYGSGRYLAEFEGVVNNLERRYRETTSD
                     WMRYEIEQYMSTADCPVCKGARLKKEALAVTVGGINIDAFTKMSVLEALDFMSSLKLS
                     DREMMIAGQIIKEIRERLSFLSSVGLEYLTLARSAGSLSGGESQRIRLATQIGSSLMG
                     VLYILDEPSIGLHQKDNEKLLKTLLKLRDLGNTLIVVEHDEDTMLAADHIVDIGPGAG
                     IHGGRLVCQGTAEDIMKCEESITGQYLSGKKKIPVPEKRRKGNGKKLIVKGAAENNLK
                     NIDVEFPLGTFICVTGVSGSGKSSLVNEILYKRLAADLNKAKTFAGKHEAILGIEHLD
                     KVINIDQSPIGRTPRSNPATYTGVFTDIRNLFASTTDAKIRGYAPGRFSFNVSGGRCE
                     ACQGDGIVKIEMHFLADVFVPCEVCKGKRYNRETLEVKYKGKNIADILDMTVEEALEF
                     FDSQPRIKNKLKTLYDVGLGYIKLGQSATTLSGGEAQRVKLSTELSKRPTGRTLYILD
                     EPTTGLHTADVHRLIDVLQTLCDSGNTIVVIEHNLDVIKTADHIIDLGPGGGNNGGTI
                     VATGTPEQVAENPNSFTGEFLKKVLKK"
     gene            complement(2975..4936)
                     /gene="uvrB"
                     /locus_tag="PLD98_00300"
     CDS             complement(2975..4936)
                     /gene="uvrB"
                     /locus_tag="PLD98_00300"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQA04733.1"
                     /translation="MGEFKLVSDYKPTGDQPQAIDALVEGVLKGYKEQTLLGVTGSGK
                     TFTMANIIARLNRPALVLAHNKTLAAQLCSEFREFFPENAVEYFVSYYDYYQPEAYIP
                     STDTYIEKDSAINDEIDKLRHSATSALSERRDVIIVSSVSCIYSLGNPIDYRSMVISL
                     RQGMTKSRDELIRKLVDIQYERNDLNFTRNKFRVRGDVVEIYPVYTNEYAIRVEFFGD
                     EVDRICEFNPLTGDIKNVVSHVAIYPASHYVISPDKLESAIEEILEEMEQQVKFFESQ
                     GKLLEAQRIRQRTEYDVEMLRETGFCKGIENYSRIMSGRKPGEAPFTLLDYFPDDFIL
                     FVDESHVTLPQVRAMYGGDRSRKEALVNFGFRLPSAYDNRPLTFEEFYQKAGQKIFVS
                     ATPGPFEREVSAQIAEQLIRPTGLLDPEVEVRSTEGQIEDLISEINIRVQRDERVLVT
                     TLTKKMAEDLTEYLEDFGIKVRYMHYDIDTIERMELLRDLRLGEFHVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETSLIQTIGRAARNANGKVIMYADTVTPSMETAIKET
                     ERRRRIQRKYNEEHGITPVTIKKDIRDIIEISTKETKEEKGKKLSPRERKALIEKLTA
                     EMKAAAKILEFEHAAYLRDRIERLRQGKD"
     gene            complement(5405..5896)
                     /locus_tag="PLD98_00305"
     CDS             complement(5405..5896)
                     /locus_tag="PLD98_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HQA04734.1"
                     /translation="MQLNFYNNEYIKTLCQRRPSATARIRGGSRYPLLRGRVNFVQAN
                     GGVLVISLIKGLPNGGERRVFGFHIHEGKSCAGTPEEPFAETMGHYNPDNLPHPYHSG
                     DMPPLFGNDGTAFSVFLTNRFKIDEIIGRTVVIHAQPDDFTSQPSGNSGEKIACGIIE
                     EIK"
     gene            complement(5943..6293)
                     /locus_tag="PLD98_00310"
     CDS             complement(5943..6293)
                     /locus_tag="PLD98_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HQA04735.1"
                     /translation="MNILWFFLGGFIIAAVYALYGLLFTITIIGIPFGKQCFKLARLS
                     LAPFGKEIVYPKFGAGSVLMNILWILLAGIETCALNFAIGVALCCTIIGIPFGIQFFK
                     LAALSLLPFGADID"
     gene            complement(6355..7602)
                     /locus_tag="PLD98_00315"
     CDS             complement(6355..7602)
                     /locus_tag="PLD98_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HQA04736.1"
                     /translation="MAVLRAFKAIRPTKDKAAAVAALPYDVLSTKEAREIVKQNPISF
                     LRIDKAEVDLPEGTDLYSDEVYLKAKENLESYINDGIFIQDEKPCLYIYRQIMDGRAQ
                     TGIVGCLSIDDYVNNVIKKHELTRADKEVDRFRHVDTCDAHTGPIFITYKGGGSIDEI
                     IAKYEKTEPEYDFVSCQGVRQIVWIVSDETDINRIIEEFKKINSLYIADGHHRSASAV
                     HVGLKRRKELGSYTGEEEFNFFLAVVFKCEDLKLMDYNRLLKDSENRSAEEIIRLSEK
                     NFYVTEKGAENFRPSEKHTFGLYLDGLWYELRAKDGTFDENDPVDRLDVTILQKNFIA
                     PVFGIEDPRTDDRIDFVGGIRGLDGLKRRVDENGWRAAVAMYPTTIDDLMSISDAGLI
                     MPPKSTWFEPKLLSGLFIHKLSD"
     gene            complement(7612..9243)
                     /locus_tag="PLD98_00320"
     CDS             complement(7612..9243)
                     /locus_tag="PLD98_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HQA04737.1"
                     /translation="MRKRFQCLLLILTLLAVSLSGCSLPNKELDLSFPVPEEAKSLDP
                     QIARLFSERIFARNAFEGLVTVDSDGKIIPGAAESCTVSDDGLTYTFKIRESARWYLT
                     NTAKQALADRLPENFDTRVTAHDFVFAFRRAVTPETACPKAELFLSIKNAAQILNGEK
                     AAEELGVEAVSDTELRITLIHPERDFLRVLTDPVFSPCSEVFFNACGGRYGLSLKYII
                     CNGPFILFRWNPGGSMRLTRNTVYSGERTVVPDVVRLFVNPDTASVLEKVTDGDYSAG
                     IITAADARNIKKPKNLKLTEKTDILWAFVFNSAKAPFSVKEMRKAFVLSTNLDSFPVP
                     EDMKGKALSPVPVSIAEAEGLKNPALLSFNEEEARALFKKAQADAEKDGAVLSGFSVL
                     CIKEHEEMLKFQLQEWQKIFGVNFSVSLIPLERAELVKRVQSGNYEAALYPLQPDTAD
                     ISSYLRSFAVESEDGIYCFNKAFINGVERLLSDGFEGDFKSNAEKLAQILTDEAVVLP
                     VFSQSRFLAVHKAVSGIYFYPSIDDVYFIKATDKK"
     gene            9466..11697
                     /locus_tag="PLD98_00325"
     CDS             9466..11697
                     /locus_tag="PLD98_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04738.1"
                     /translation="MKFDFTGLSIAAVTEEEKKAAEILKDEINTRTGHAPAVSEKAEG
                     KAVVFKTDNEGEVPHKDSYKLSLCGETLYIIAKGIRGFIFGIGYFLRKTEYKNEKITL
                     IKDISGLYVPDKRIRGHQLGYRTTPNTYDAWTYDDYVRYYKDMMFFGCNMCEHIPYEK
                     AVSNRNHLMKYDEEEFLIEASRLADELDLDVSIWFPNCEDTLEEAVERRRELFKKIPR
                     INAVFPPGGDPGDLEAEEFVRRAIAFGKVLKEFHPNAGMWPSAQKPKKILSWGDDFIE
                     EMEKLPDEIEGIVTGPNRAFPLHELRRLLPTKYPIRLYTDITHNVRCEYPVHYPYDDW
                     HYSLTSCLSRESVNPRPAEYEMIHRLTRGYIVGSVSYSEGVNDDLNKMVWSNLDFFPN
                     VSIRETIEDYARLFFPGAPADLVTEGLLGFEHNWMGDPVNNPGIEATLKIWQGLASDY
                     PKLMDNWRFVMHLFRAECDAIVRRRRIFELNLIDDALYVLNKEGLEAALKVLEETEFD
                     DEYKSLREHIEVLAEKLFNLIGMQLDVARYGANSWERGATLETIDLPVTDREWLIGRF
                     KHALTLPESERDGFVHRVINRNKVGPEEFYYSFAEHGFDMLGVRQEGEVYINFQGDRP
                     ENRGKMPMSMLKVFDNLTFRCKLGGFAENTDYKLRITFKSDKHPIFNRHYIIANGVEI
                     YKGPQFGGERDEKFDEELLAPGFETATYLLPASVFKNGTLELEFGEPLMGVMMCEFWI
                     TKA"
     gene            11756..13357
                     /locus_tag="PLD98_00330"
     CDS             11756..13357
                     /locus_tag="PLD98_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04739.1"
                     /translation="MKKKTAAMLSAAAFAAGSALTLKLQQELKKDENKRVLYDKGNKT
                     FYGALKGIASVLPEPPMVHLVDYVSENFYKGHDEFIDKPKKGAKWRLGFACDSIIPDD
                     ISTGEYYMGGYLSIPANRVEGVLDGLMFRCIAVDDGNGRGTVVFGVIDSIGLSAGDIR
                     EIRKRLSDFAKEHNIISINISASHCHSGIDTQGLWGNLKEIFPTNYKAVKNREFHKLK
                     SGKNPKHMEMIFNIAVDSIKRAVESMEPGTLYSATVPGRGYARDKRPPNVVVDEITTF
                     KFVPDDKDKRPTRLVLMAAHPTSFPDKNKMVSGDYPYHICNKLHKLGENAVFFQGPQA
                     SIASNKSAFTASDSKEDDPPYKKMGEGVAEYLYNLPEDEYEEVKPILNIRHREVVLPV
                     TNYLYHILGKLMIVTTIMVKTSGRKDDLAVITEIGYAEVGDKIKFALIPGELMPEILL
                     GGFYSHEEAYTRTDWAMPSLKDIAGEKLIGIGLCNDAIGYIVPDNDYGSIFAPYHYEE
                     SVSVGEKTASTIVTEFMKMVEEVRG"
     gene            13504..14181
                     /locus_tag="PLD98_00335"
     CDS             13504..14181
                     /locus_tag="PLD98_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQA04740.1"
                     /translation="MKYLYEMHLHTSEVSGCGRVSGADSAVFYKEKGYAGIIVTDHMV
                     PRAFRGMEDASWDEKVDRFMEGYKKAVQAAKDENFDVILSMELRFMSESENDYLIYGF
                     TEEWLRNNPEPYNLTLKQFKKIADENGFLIYQAHPFRDHMTVVDPKYLHGIEVHNGHH
                     GHDSRNPIAEAWAELHGLPQSSGSDYHYLEHLARGGMLFYERIRTPQDLVKQLKSGRY
                     GLFKGGF"
     gene            14227..15264
                     /locus_tag="PLD98_00340"
     CDS             14227..15264
                     /locus_tag="PLD98_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQA04741.1"
                     /translation="MKNFRRAVSLLLVLTVLCFSVSFAAFAEGPENEGGLLTVMSYNV
                     SGLPFAGAVQGSEIVPGYPKAELLGALMNQVDADIIGVQEDFNFHDSLAAKMTNFPYK
                     TFSSGPIPLGDGLNIFSKLPIYNISRHTWEKRYGVFKGAADQLTPKGFLYSVVEIAEG
                     VYIDFYVLHADAGGDPQSTAARIDNYRQLTEHINTRPHDRAVIVVGDFNARILSGFET
                     DMLNNLIFPAGLSDSWVELCNNGVYSTEPQFEEYEVFESIDKVMYKNGGGVEFTPVDI
                     EHYKLKNERGGTYTDHHATKVVLSYTVNEIVPMNEELAEPTPHDTGFMAMFKAVLTAL
                     KLLISDLGNLF"
     gene            complement(15290..15838)
                     /locus_tag="PLD98_00345"
     CDS             complement(15290..15838)
                     /locus_tag="PLD98_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HQA04742.1"
                     /translation="MKTLLHICCAPCSVMCVDTLRNEGIEPVGYWFNPNIHPFTEYKQ
                     RREALKEYAEKTGLELVLEDYYNLREFVINVTPEFDSRCVYCYETRAEKAAEYAADNG
                     FEAYTTTLLISPYQNHELLKEVFEKTGEKYGVRFLYRDFRPYFREGQQRAREMGIYMQ
                     KYCGCVFSEEERYNKKLFKNKK"
     gene            complement(16192..16932)
                     /locus_tag="PLD98_00350"
     CDS             complement(16192..16932)
                     /locus_tag="PLD98_00350"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQA04743.1"
                     /translation="MSYTGKYFEILHQKLDELLKTQEENIKALAIKLVDTVESGGKVF
                     FFGCSHAGILAQEAFYRTGGLVIANPILPKGLTLDTVPVTDTSVLERKSEYAHEILNS
                     VPISKGDLLVIHSVSGRNGVPVEMAIEARKRGIYTVAITSVKYSSAQSSRHESGKRLF
                     EVCDMVIDNVGEIGDAVLTIDGFAEKTAPTSTIMGAAIINSLCAESVAEFLKRGIDPP
                     VFRSANLDGGDEYNARMFEKYKDRITYM"
     gene            complement(16982..17404)
                     /locus_tag="PLD98_00355"
     CDS             complement(16982..17404)
                     /locus_tag="PLD98_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HQA04744.1"
                     /translation="MKNSIKLFAASLIIVSIVFAFLLNASTVLAGDVDKNGKVQASDA
                     RLVLRHVAKLQTLSKEQEFIADVDQNGKVTAADARLILRMVAKLDKEFELTTKGSATT
                     TKPTTTTKPSGPTTTLPQVNLVTDNGAADGEITLFGGN"
     gene            complement(17651..20059)
                     /locus_tag="PLD98_00360"
     CDS             complement(17651..20059)
                     /locus_tag="PLD98_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HQA04745.1"
                     /translation="MENSFKRAVKVLELDKILEKLAGLTACSDAYEMALAIMPETRLD
                     RANFLLEQTKDAYALIARFSAPSFSGLKNVDNALARAAAGSVLSTRELLDIAESLRVI
                     RALSEWRRNCGGGSSLDFLFSELIPNKALEESITKAIISEDEIADTASPALADIRRKI
                     RRKTAGIKEQLESLIRSAHYRKILQEPIVTQRNGRYVVPVKAEHRGEVAGLVHDTSSS
                     GATVFIEPSAVVEANNEIRMLLSEERDEIERIITYLSSEAGSFAAPIKQSTKAAAELN
                     LIFAKAALAYSMKASAPELNDKGLIELKRARHPLIPQDKVVPIDLKLGFDYDALIITG
                     PNTGGKTVSIKTLGLLTLMAMCGLFIPASENSKVCVFKGVFADIGDEQSIEQSLSTFS
                     SHMTNIVKIIEECSEGSLVLIDELGAGTDPVEGAALAIAILEHIRRKGAKIAATTHYA
                     ELKAYALQTERVENASCEFDVNTLKPTYRLLTGIPGRSNAFAISEKLGLPMEITEKAK
                     SLVSGENIKFESLVTALEEKRFEAEKKLRQALEEKREAERLLSEAKERLEKAIQQSEK
                     ELEKARTEAIRLADRAKREAADFMAELERLRKESEEIKQHGGAAELKRKLRSSINALE
                     DSLNPVSMSARSEAAFDEPPETLKIGDRVLLVDMGCEGVVSSYDEKSENAEVQIGGIK
                     MRVAKERLRLIAGKAKKEKSAQSTYNTIKSVKSEHKVLASQDTRCDIRGMTVDEALPV
                     VDLAIDSLVIAGLHEITIIHGKGTGKLRAAVHQHLRKHPQIKTFRLGTFGEGEDGVTI
                     AELK"
     gene            complement(20108..20857)
                     /locus_tag="PLD98_00365"
     CDS             complement(20108..20857)
                     /locus_tag="PLD98_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQA04746.1"
                     /translation="MTKIIAHRGASKAAPQNTLAAFKKAKAMGAHGFENDVHLTKDGV
                     IVVCHNPTIDATSNGTGAICDMTLEELRQYDFGSYFSPEFAGEKIPTLREFYEVAQGL
                     EIVNVEIKKPANNDLSIVKGVIDLAKECGCYENLLISSFSEEVLKEARRLDDKVMTGV
                     LYDPVCEIAAKVIPDPIGYAKSVGATHLHPVFLIVDEDFIEEAHKNDLKVNAWTINTD
                     YVIKTFIEWGYDGIITDVPDLALKILNDFSK"
     gene            21111..22727
                     /locus_tag="PLD98_00370"
     CDS             21111..22727
                     /locus_tag="PLD98_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HQA04747.1"
                     /translation="MFTVPNLKLKINKIIGSILVAILSVGYYLGLSFDIQGVRYDKSS
                     VLTYCFYDSTYKLGKNEFIGKNETYNIHLAKNEYEACQIAVRSKMNSTHRKYTIEFSQ
                     FVNENGDVLPSEVFEEKYIACVSDKNYGTFPDALVKYAPAPFSLSPQQNKVFYIQVHA
                     GKDTPAGTYKATITARSSGHDNDKVQFIAEVTATVWNFELPDTPSCETAFGLSKYNIV
                     NAYYANNDPERAQALYEQYYEYLVNRKISPYNLPVDILSDEADAYMSDPRVTSFIIPY
                     YSDDATLVAAYNKVQSNPEWAKKGYFYPIDEPSSLEDYGRYNAIVERLDRLCPGFKMV
                     TPFYQASIKEGGKTYHATELQSASKIVCPLTSCFNNKTFLEQIDKRRADGSKIWWYVC
                     CSPQGSYCNIFTHWEGIKGRLLLWQQKQFNVQGLLYWDTTYWKDVVSPWGDALTTPWT
                     GNDTFGDGSLLYPGQDGPVASLRLEEIADGIEDYEYLTIAEELFGRDYINNKIAKVTN
                     TLTDYTLDDSLLAKVRVEIGNDINNAMSGN"
     gene            22786..24630
                     /locus_tag="PLD98_00375"
     CDS             22786..24630
                     /locus_tag="PLD98_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HQA04748.1"
                     /translation="MFDSFKSLSGAAKGLSQNLKVIAEYGSVSKILEDIKTEGGEIKE
                     IQFVLISPDELGKKAAEIFPDAPAPISDAYIILSEGDKLTAYSSTKAGLHFAAGAIKR
                     HILCGGIKEGEIYNTPDVPFRCVKMYLPAREDIPVFFEIIELCVALGCNAVMLEIGGA
                     MEYKLHPEINEGWVEYCDMFRDEPGATDKFKAQFDFHKNSIHSENGGGYFLTQSEVRA
                     LADYCEERGLEVIPEVPSLSHCDYLLTRHKELGERREDPWPDTYCPSRKESYELIFDV
                     LTEVIEVFRPKRINIGHDEFYTLKLCPLCKDKDGSEIFAEDINKIHAFLKSKGVGTVM
                     WGEKLLAFCARDGQPMGGMERYKERPNLYIPETYKARDLISKEIEILHWYWGVNKECE
                     DQLYDRGFTLYFGNFSAVAMDDWKGRLKRGVKGICISNWSRLDILHMQRNTVLLEMAL
                     SSMMIWNPNYSEKNFAENIIKASDLLYRLKNYKTLRGKHIEIRHAVNLRHEHKPFVDG
                     NFIDYEKDYLGEYEIVCKNGEKLTVPIYYGLNIGCMDGRWVMTPSSRARYVGNEFDTH
                     ILEPAYTCRIERDGGKTFYVIAVSVNDDVESIKLNTHLDPSFFEWQVL"
     gene            25603..25914
                     /gene="rpsJ"
                     /locus_tag="PLD98_00380"
     CDS             25603..25914
                     /gene="rpsJ"
                     /locus_tag="PLD98_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQA04749.1"
                     /translation="MAVKEKIRIRLKSYDHNLVDKAAEKIVETAKRTGARVSGPVPLP
                     TEKEVITILRAVHKYKDSREQFEQRTHKRLIDILRPSNKTVEALTELQLPAGVDIEIK
                     L"
     gene            26021..26656
                     /gene="rplC"
                     /locus_tag="PLD98_00385"
     CDS             26021..26656
                     /gene="rplC"
                     /locus_tag="PLD98_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQA04750.1"
                     /translation="MQKGLIGKKIGMTQLFDEKGNVIPVTVIELGPCPVVQKKTVETD
                     GYDAVQLGFGEVKVQRVNKPVKGHFAKANVAPRKYLKEFRLDDVSSLNVGDIIKADIF
                     EVGEKVDVTGITKGKGTAGVIKRWNFSRLKESHGSGPVVRKGGSIGSNSDPSRVFKGK
                     RMAGRLGHEKVTIQNLDVVKVDAENNLIAVKGAVPGPRGGIVFVTDNVKKA"
     gene            26671..27294
                     /gene="rplD"
                     /locus_tag="PLD98_00390"
     CDS             26671..27294
                     /gene="rplD"
                     /locus_tag="PLD98_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQA04751.1"
                     /translation="MPIVPVYNAAGEKIDDITLKDEIFAIEPNTHAVHLAVVSYLANQ
                     RQGTQSTLTRAEVSGGGRKPWRQKGTGRARHGSIRAPQWRHGGVALGPKPRDYVKQVN
                     KKVKRLALKSVLSAKLSADEIKVLDSFGLEAVKTKEALKVLKNLDTGKKTLIVLPEKD
                     DIVYLSTRNICGVKTVTVDSLNVYDLVNCNTVLFLRNAVTALEEVYA"
     gene            27291..27587
                     /gene="rplW"
                     /locus_tag="PLD98_00395"
     CDS             27291..27587
                     /gene="rplW"
                     /locus_tag="PLD98_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQA04752.1"
                     /translation="MSKLPQDIIIRPIITEESMKGAPVKKYAFQVAKDANKIEIAKAV
                     EKLFGVEVAKVNTMNVKGHLRRYGRYEGYRPNWKKAVVTLTEKSKSIEFFDGLL"
     gene            27729..28562
                     /gene="rplB"
                     /locus_tag="PLD98_00400"
     CDS             27729..28562
                     /gene="rplB"
                     /locus_tag="PLD98_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQA04753.1"
                     /translation="MSVKVYKPTSNGRRNMSVTDYSVLSKVEPEKSLLEPLRKKSGRN
                     SYGRITVRHRGGGNRRKYRIIDFKRSKFGVPAVVQTIEYDPNRTAHIALIQYEDGEKS
                     YILAPEGLKVGDTVVAGPDADIKVGNALPLANIPVGTFVHNVEMHPGRGGQIARAAGQ
                     AAQLMAKEGNLALLRLPSGELRNVPAECIATIGNVGNQEHENVKIGKAGRTRHMGRRP
                     TVRGSAMNPVDHPHGGGEGKAPIGRPGPVTPWGKPALGYKTRKKKNKSNKFIVKRRNQ
                     I"
     gene            28584..28865
                     /gene="rpsS"
                     /locus_tag="PLD98_00405"
     CDS             28584..28865
                     /gene="rpsS"
                     /locus_tag="PLD98_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HQA04754.1"
                     /translation="MGRSVKKGPYVDKKLLAKVRQMNEKGEKIVIKTWSRSSTIFPDF
                     VGHTFAVHDGRKHVPVYVTEDMVGHKLGEFAPTRTFRGHAGSKTSNTGK"
     gene            28888..29223
                     /gene="rplV"
                     /locus_tag="PLD98_00410"
     CDS             28888..29223
                     /gene="rplV"
                     /locus_tag="PLD98_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HQA04755.1"
                     /translation="MEATAKATFLRVAPRKIQIVLDLIRNKSAEEALAILKYTPKAAC
                     EPLLKLLKSAIANAENNHDMNVASLYVAECYVSQGPTLKRIRPRAQGRAYRINKKTSH
                     VTLVLKERE"
     gene            29242..29937
                     /gene="rpsC"
                     /locus_tag="PLD98_00415"
     CDS             29242..29937
                     /gene="rpsC"
                     /locus_tag="PLD98_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQA04756.1"
                     /translation="MGQKVNPTGLRIGVIKDWESKWYADKKTFSNNLIADYKLREYLY
                     ETLKAAGVPKIEIERDTRRVRVNIHCAKPGIVIGKGGAEIEKLKAQCKKILGTDEVII
                     NIVEVKQPDLNAQLVAENIAAQLERRISFRRAMKQAIGRTMRLGAKGIKTQVSGRLGG
                     AEIARTEHYHEGTIPLQTVRADIDYGFAEAHTTYGRIGVKVWIYQGEVFHDTRSNRNK
                     REGRAGHAATKAR"
     gene            29915..30343
                     /gene="rplP"
                     /locus_tag="PLD98_00420"
     CDS             29915..30343
                     /gene="rplP"
                     /locus_tag="PLD98_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQA04757.1"
                     /translation="MLLPKRVKYRRVQRGRLKGAASRGTKITYGDFGLVALEPAWITS
                     NQIEAARVAMTRYIRRGGQVWIKIFPDKPITEKPAETRMGSGKGSPEYWVAVVKPGRV
                     MFEIGGVSEELAREAMRLAAHKLPIKCKFVTKKEQDGEQE"
     gene            30340..30543
                     /gene="rpmC"
                     /locus_tag="PLD98_00425"
     CDS             30340..30543
                     /gene="rpmC"
                     /locus_tag="PLD98_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQA04758.1"
                     /translation="MKASEFRKMTAQELDAKLLELKDELFKLRFQQTINQLENPMRIR
                     AVKKDIARLLTVKRDKELHGSES"
     gene            30564..30818
                     /gene="rpsQ"
                     /locus_tag="PLD98_00430"
     CDS             30564..30818
                     /gene="rpsQ"
                     /locus_tag="PLD98_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HQA04759.1"
                     /translation="MERNLRKTRVGVVASDKMDKTVVVAVKDRVKHPLYKKIVNRTIR
                     LKAHDENNECRVGDRVLIMETRPLSKEKRWRVAEIIEKAK"
     gene            30832..31200
                     /gene="rplN"
                     /locus_tag="PLD98_00435"
     CDS             30832..31200
                     /gene="rplN"
                     /locus_tag="PLD98_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HQA04760.1"
                     /translation="MIQQQTLLKVADNTGAKEIMCIRVLGGSGRKYANIGDVIVASVK
                     KATPGGVVKKGDVVKAVVVRSAFGVHRNDGTYIRFDENAAVIIRDDKNPRGTRIFGPV
                     ARELRDKDYTKILSLAPEVL"
     gene            31216..31545
                     /gene="rplX"
                     /locus_tag="PLD98_00440"
     CDS             31216..31545
                     /gene="rplX"
                     /locus_tag="PLD98_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQA04761.1"
                     /translation="MKKKVHVKTGDTVLVIKGKDRGKTGKVLEVSPEEGKVIVEGINI
                     VTKHVKPRRMGEPGGLIKAEAALYADKVQLVCPKCGKPTRVGHAFDSEGKKVRVCKKS
                     GCAAQFE"
     gene            31572..32111
                     /gene="rplE"
                     /locus_tag="PLD98_00445"
     CDS             31572..32111
                     /gene="rplE"
                     /locus_tag="PLD98_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQA04762.1"
                     /translation="MPRLKELYLNEVAPALMKKFNYKSVMQIPKLDKVVINVGCGEAR
                     DNPKVLDHVVSDIAQITGQKPVVVKAKKSVANFKIRAGMPNGVKVTLRGDRMYEFLDR
                     LFNLALPRVRDFRGINPNSFDGRGNYALGVKEQLIFPEIDYDKIDKVRGMDIAIVTTA
                     KTDEEARELLALMGAPFTR"
     gene            32127..32312
                     /locus_tag="PLD98_00450"
     CDS             32127..32312
                     /locus_tag="PLD98_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HQA04763.1"
                     /translation="MAKTSMIVRSQRPAKYSTRQHNRCKICGRPHAYLRKYGVCRICF
                     RELAHKGQIPGVKKASW"
     gene            32342..32740
                     /gene="rpsH"
                     /locus_tag="PLD98_00455"
     CDS             32342..32740
                     /gene="rpsH"
                     /locus_tag="PLD98_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQA04764.1"
                     /translation="MQITDSIADMLTRIRNATSAKHDTVSVPASNIKKAIAQILLDEG
                     YIKGFKVIDDGKQGVIEIALKYGPNKTSVITGLRRVSKPGLRIYTNCENMPKVMRGLG
                     IAIISTSKGVMTDKDARKLNVGGEVLAFIW"
     gene            32754..33302
                     /gene="rplF"
                     /locus_tag="PLD98_00460"
     CDS             32754..33302
                     /gene="rplF"
                     /locus_tag="PLD98_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQA04765.1"
                     /translation="MSRIGKKPIPVPAGVEVNIAGNVVTVKGPKGTLTVNAHRNMKVA
                     LENGEIIVTRPNDDKENRALHGLTRSLIANMVEGVTNGFKKELEINGVGYRAAAQGGK
                     KITLNVGYSHPVDLEAPAGVTVETPAPNKIVITGADKQAVGEFAAQVRKVRPPEPYHG
                     KGIKYSDEVIRRKEGKAGKGGK"
     gene            33317..33676
                     /gene="rplR"
                     /locus_tag="PLD98_00465"
     CDS             33317..33676
                     /gene="rplR"
                     /locus_tag="PLD98_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQA04766.1"
                     /translation="MVNRPDSNKARIKRHKRVRAKVKGTPECPRLNVFRSLKHIYAQL
                     IDDVNGVTLVSASTVEKGFEGYGGNVEAARKVGKLIAERAAEKNIEKVVFDRGGYIYH
                     GRVKSLAEGAREGGLKF"
     gene            33694..34197
                     /gene="rpsE"
                     /locus_tag="PLD98_00470"
     CDS             33694..34197
                     /gene="rpsE"
                     /locus_tag="PLD98_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQA04767.1"
                     /translation="MAKFEAQEKSEFKERLVAINRVAKTVKGGRIFKFSALVVVGDEN
                     GTVGFGLGKSGEVPDAIRKAIEDAKKNLIKVSLKGTTIPHEVIGKFGSGVVLMKPAAP
                     GTGVIAGGAVRAVVETVGIKDIRTKALRSSNPCNVVRATIDGLKQLRNADEVAAIRGK
                     KVEEIIG"
     gene            34210..34389
                     /gene="rpmD"
                     /locus_tag="PLD98_00475"
     CDS             34210..34389
                     /gene="rpmD"
                     /locus_tag="PLD98_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HQA04768.1"
                     /translation="MANIKVKLVRSLIGSTKPQIATAHALGLRKVGDESVQPDNPQTR
                     GKINKINHLVEIADA"
     gene            34405..34848
                     /gene="rplO"
                     /locus_tag="PLD98_00480"
     CDS             34405..34848
                     /gene="rplO"
                     /locus_tag="PLD98_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQA04769.1"
                     /translation="MKLHELSPAPGSTKDAKRVGRGHGSGHGKTSGKGHKGQKARAGK
                     GPRVGFEGGQMPLQRRVPKRGFNNIFATKFAVVNLADLERVFSDGQVVDIEALRQAGL
                     IKKPLDGVKVLGNGELSKKLTVKVNAYSETAKAKIEAAGGTAEII"
     gene            34898..36220
                     /gene="secY"
                     /locus_tag="PLD98_00485"
     CDS             34898..36220
                     /gene="secY"
                     /locus_tag="PLD98_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQA04770.1"
                     /translation="MFKTIANAWRIEDIRKKLLYTALILFIFRIGAAIPVPFVNAAEG
                     IISNTDTSSFISYLIMMTGDAFNYGTIFAMSITPYINASIILQLLQVVIPALERLAQE
                     GEEGRKKISAITRYTAVGLGLLQSIAYYVYLRSGGFLTTDDAGKKFAGFPAVLQALVI
                     IGILTAGSALVMWLGEQINEKGIGNGISLILFTGIISRIPTLVTTLFQYFNRGGVFYG
                     LAPLAAVIMILMVAFIVWMDNAEHRIPVQYAKRVVGRKMYGGQSSHIPLKVNMSGVLP
                     VIFASSILSIPPTIQLFVKAENKFWTTILGWFNQTSPLYVVIYFFMIIFFAYFYSQIQ
                     YNPIEMANNLRKNSGAIPGIRPGEPTAAYIAKIMSKITLLGALLLSVVALFPIIFTQL
                     TTLIFGEGRTMNISLGGTSVIILVGVALETVQQLESQMMMRHYKGFLD"
     gene            36242..>36588
                     /locus_tag="PLD98_00490"
     CDS             36242..>36588
                     /locus_tag="PLD98_00490"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HQA04771.1"
                     /translation="MRIIFLGAPGAGKGTQAETVSRKLNIPTISTGNIIRAAIKDGTK
                     MGLAAKEYTDKGLLVPDDVVIGIIKERLSEDDCKEGFILDGFPRTIPQAESLDQMGIE
                     IDKVINIHVPDET"
BASE COUNT         9448 a         9360 c         9077 g         8703 t
ORIGIN      
        1 cgaacgccgc cccgcccaca gccgcccgcc ccgccgccga gaaaatcccc ccccccgaca
       61 ccaaaaaaac cgcccccaac gggcgggcag aaaaacaaaa cagcggaaaa ccactaaagg
      121 aattctccgc tgtaatccta tttctttaat acctttttaa gaaactcgcc tgtaaagctg
      181 ttggggtttt cggctacctg ctcgggcgtg ccggtggcaa caatcgtgcc gccgttattg
      241 ccgccgccgg gacccaagtc aataatatgg tccgccgtct taatcacatc cagattgtgc
      301 tcgattacca caatcgtgtt gcccgagtcg caaagggtct gaagcacatc aatcagcctg
      361 tgcacatcgg cggtgtgcag tcccgtggtc ggctcgtcca gaatataaag ggttctgccc
      421 gtggggcgct ttgaaagctc cgttgacagc ttgaccctct gagcctcgcc gcccgagagg
      481 gtggtcgcgc tctgaccgag ttttatatag cccagtccca catcgtaaag ggttttcagc
      541 ttatttttaa tcctcggctg actgtcaaag aactccagcg cctcctcgac ggtcatgtcc
      601 agaatgtcgg cgatgttctt gcccttgtat tttacctcaa gggtctccct gttgtacctc
      661 ttgcccttgc agacctcgca ggggacgaaa acatcggcta aaaaatgcat ctcgattttc
      721 acaatgccgt cgccctgaca agcctcgcac ctgccgcccg acacattgaa ggagaacctg
      781 ccgggagcgt agcccctgat tttcgcgtcg gttgtggagg cgaaaagatt tcgaatatcg
      841 gtgaaaacac ccgtataggt ggcggggttt gagcggggag ttctgccgat gggcgactgg
      901 tcgatattga ttaccttgtc aagatgctca attcccaaaa tcgcctcatg ctttcccgca
      961 aaggttttag ccttgttgag gtccgcggcg agacgcttat aaagaatctc gtttaccagc
     1021 gacgacttgc cgctgccgga aacgcccgtc acgcagataa atgtgcccag cgggaactca
     1081 acatcaatat ttttaaggtt attctccgcc gcgcccttta caatcagctt tttgccgttg
     1141 ccctttctcc tcttttcggg gacggggatt ttctttttgc cgctgaggta ctgtcccgta
     1201 atggactcct cgcacttcat aatgtcctca gcggtgccct gacagacaag gcgtccgccg
     1261 tgaatgcccg cgccggggcc gatgtccaca atgtggtcgg cggctagcat ggtgtcctca
     1321 tcgtgctcca ccacaataag ggtgttgccc aaatccctga gtttcaaaag cgtctttaac
     1381 agcttctcat tgtctttctg atggagtccg atgctgggct catcgaggat atacaaaact
     1441 cccatgagtg atgagcctat ctgcgtggcg aggcggattc tctggctctc gccgcccgaa
     1501 aggctgcccg cgcttctcgc cagagtcaga tactcaagac ccacgcttga aaggaaggag
     1561 agcctttccc taatctcctt tataatctgc cccgcaatca tcatttccct gtcggagagc
     1621 ttgaggctgc tcatgaaatc cagagcctca agcaccgaca tttttgtaaa ggcgtctatg
     1681 tttatgccgc ccaccgtcac cgcaagagcc tcctttttga gccttgcgcc cttgcagacg
     1741 gggcagtcgg cggtggacat atactgctca atctcgtatc tcatccagtc gctggtggtc
     1801 tccctgtacc tgcgctcaag gttgttcaca acgccctcaa actcggcaag gtacctgccg
     1861 ctgccgtact ccatggttct ctcaaggtgg attttctcgc cctttgtgcc gtagaggatg
     1921 atgtcgataa tctccttggg cagcttttcc acgggagtgt ccagcgaaaa gccgtagtgc
     1981 tttgcaagac ccctgaaata catggtggct atggagctgg ggtccgcgga gttccatccg
     2041 cttgccgtaa tcgcgccctc gtttatggag agctttttgt tcggtataac aaggtcgggg
     2101 tttaccttca tctgaaagcc cagacccgcg cactcggggc aggctccgaa cgggctgttg
     2161 aaggagaaaa gacggggagt cagctcgtca atgcccgcgc cgtgaatggg gcatgcccag
     2221 ttttgggaga acataagctc ctcgccgtcc acaatgtcta tgagcacagt gcccgacgcg
     2281 agtttggagg caagctccac cgagtcggca agcctgcttt taattccttc tttaattaca
     2341 agcctgtcaa caataatctc aatattatgc tttttgttct tgtcgagctt aatactctcg
     2401 gacaagtcat aaatgctgcc gtcgattctg gctcttacat agccgctttt cttcgcgctt
     2461 tcaagctctt tggcgtgttc gccctttttg ccgcgaataa tcggcgccat gacctgcagc
     2521 cttgtgccct cggggtagga aagtatcctg tccacaatct ggtccacggt ctgctgtttt
     2581 atctccctgc cgcactcggg gcagtgggga acgccgattc tggcgtacaa aagccttaaa
     2641 tagtcgtaaa tctccgtagc tgtgccaaca gtggagcggg ggtttttcga ggttgttttc
     2701 tggtcgatgg aaatggcggg ggaaagcccg tcaatatagt ccacatcggg cttttccatc
     2761 tgacccagaa acatacgggc gtaagccgaa agcgactcca catagcggcg ctgaccctcg
     2821 gcgtaaattg tgtcaaaggc aagggacgac ttgccgctgc ccgaaagccc cgtaaaaacc
     2881 accagcttgt ccctggggat aaccacatca atatttttaa gattgtgctc tctggcaccc
     2941 tttataacaa gatttctctg cataaaaacc tctttcagtc ttttccctgc cttagtcttt
     3001 ctattctgtc acgcagataa gcggcatgct cgaactcgag tatctttgcg gccgccttca
     3061 tctccgcggt tagcttttcg ataagagcct ttctctctct gggggagagc tttttgccct
     3121 tttcttcctt tgtctccttg gtggagattt cgataatatc cctgatatcc ttcttgattg
     3181 tgacgggagt aatgccgtgt tcctcgttgt attttctctg aatcctgcgc cttctctcgg
     3241 tctccttaat cgcggtttcc atggaggggg tgacggtgtc ggcgtacatt atgaccttgc
     3301 cgttggcgtt tctcgccgcc ctgcctatgg tctgaatcag cgatgtctcg gagcggagga
     3361 atccttcctt gtccgcgtca agaatcgcca ccaaagatac ctcgggaagg tcaagaccct
     3421 cgcgcaaaag atttatgccc acaagcacat ggaactcgcc gagccttaaa tcccttaaaa
     3481 gctccatgcg ctcgattgtg tcaatgtcgt agtgcatgta tctgaccttg atgccgaagt
     3541 cctcaaggta ttcggtcaaa tcctcagcca tcttctttgt aagggtggtg acaagaaccc
     3601 tctcgtcacg ctgaaccctt atattaatct cggaaataag gtcctcaatc tgaccctctg
     3661 tggagcgaac ctcaacctcc gggtcaagaa ggcccgtggg acgaatgagc tgttcggcaa
     3721 tctgcgcgct gacctcccgc tcaaagggtc cgggagtcgc gctgacaaag attttctgcc
     3781 ccgccttctg ataaaactcc tcaaaggtaa ggggtctgtt gtcatatgcg ctggggaggc
     3841 ggaatccgaa gtttacgaga gcctcttttc ttgagcggtc gccgccgtac atggctctga
     3901 cctgggggag cgtcacatgg gactcgtcaa caaaaagtat aaaatcatcg gggaaatagt
     3961 caagcagcgt aaagggagcc tcacccggct ttctgcccga cattatcctt gagtagtttt
     4021 caatgccctt gcaaaagcct gtttctctca gcatttccac atcgtactcg gtgcgctgcc
     4081 ttatcctctg agcttcaagg agcttgccct gactctcaaa gaacttaacc tgctgctcca
     4141 tctcctcaag aatctcctcg attgcgctct ccagcttgtc gggggagatt acatagtggg
     4201 aggcggggta gatggccaca tgggagacca catttttaat gtcgcccgta agggggttaa
     4261 actcgcagat gcggtccacc tcgtcgccga aaaactcaac tctgatggcg tactcgtttg
     4321 tatagacggg gtaaatctcc accacatcgc cccgcaccct gaacttgttg cgggtaaagt
     4381 tcaggtcgtt gcgctcgtac tgaatgtcaa cgagttttct tatcagctcg tcgcgggatt
     4441 tggtcatgcc ctgtctgaga gatatgacca tgctgcggta gtcaatcggg ttgccgaggc
     4501 tgtatatgca ggaaacgctg gatacgatta tgacatctct ccgctccgac aaagcagagg
     4561 tggcggagtg gcggagcttg tctatctcgt cattgatagc cgagtccttt tcgatatatg
     4621 tgtccgtgga ggggatgtat gcctcgggct gatagtagtc gtagtaggaa acaaagtact
     4681 ccacggcgtt ttcggggaag aactccctga actcgctgca gagctgagcc gcaagggttt
     4741 tattgtgggc aagcacaaga gcggggcggt taagccgagc gataatgttt gccatggtga
     4801 atgtcttgcc ggagcccgta acgcccagca gagtctgctc tttgtatccc tttaaaacgc
     4861 cctccacgag agcgtctatt gcctggggct ggtcgcccgt cggcttataa tcggatacca
     4921 gtttgaattc gcccaaataa aagccccctt tgatgaacta aaattagtat accaaacccg
     4981 acctggaaaa gcaaacattt gtttgcacaa atttaggatt ttttcccttc tgttatgtgt
     5041 aaaattttac tctttacagc atttgcgcaa agtgattgtt caaagccacg ccgctgacga
     5101 gaatacccgc cgagccgcac ctgccgcccc ctgctcgcta ctgaccgccg agctgccctg
     5161 ccaccgcccc gccgattcgc cacctgcccg ccgcaccctc accgccaagg ggagtgtggt
     5221 ccgcattttt tatttttctc ccgccgccct gccgttgggg gagcaacgcc gcccgctccg
     5281 ctgaccccac tgcctgccgc aacgctctca ccccgcagct gggggagtgc tcccgccccc
     5341 ctgcgaacgc aaaaaaaccc tcccgaagtg gagggcggca aaaaacccgc cttgagcggg
     5401 ttttttattt gatttcttca attattccgc aggctatttt ttcgcccgag ttgccgctgg
     5461 gctggcttgt aaaatcgtcg ggctgcgcgt gaattaccac cgttctgcct ataatctcgt
     5521 caatcttaaa gcggttggtc aaaaagaccg aaaatgctgt gccgtcattt ccaaagaggg
     5581 gcggcatatc cccgctgtgg tacgggtgcg ggagattgtc cggattgtag tgccccatgg
     5641 tctcagcaaa aggctcctcg ggagttcccg cgcaggattt gccctcgtgg atatgaaagc
     5701 cgaaaactct cctttccccg ccgttgggca gaccctttat aaggcttatc accagcaccc
     5761 cgccgtttgc ctgcacaaag tttaccctgc ctctgagcag cggatagcgg ctgccgcctc
     5821 ttattctcgc cgtcgccgac ggccgtcttt gacaaagcgt ctttatgtac tcgttattgt
     5881 agaaatttaa ctgcaaaaaa atcacccccg tatatcttat gaagaagggg tgatttaggt
     5941 gcttaatcta tgtctgcgcc gaaaggcaac agcgacagcg ccgccagctt aaagaactga
     6001 attccgaatg ggatgccgat aatcgtacag caaagggcaa cgccaatcgc aaagtttaaa
     6061 gcgcaggttt caatgcccgc aagcagtatc caaaggatgt tcatcagtac gctgcccgcc
     6121 ccgaatttgg gatagactat ctcctttccg aatggggcga gggacagcct tgccagctta
     6181 aagcactgct tgccgaaggg gattccgatt atggtaatcg taaacaacag cccgtagaga
     6241 gcgtacaccg cggcgataat aaagccgcct aaaaagaacc aaagtatgtt cataagaacc
     6301 ctccctttcc tcgggcagcc ccttaatatt ataagcggat ttcttcccgt tatgtcagtc
     6361 cgacagcttg tgaataaaga gacccgaaag gagcttgggc tcaaaccatg tggatttggg
     6421 aggcattatc agcccagcgt cggaaatgga catcaggtcg tcaatggtgg tggggtacat
     6481 ggcgacggcg gctctccagc cgttttcgtc aaccctcctt ttaagaccgt caagacctct
     6541 tattccgccc acaaagtcaa ttctgtcgtc ggttctcggg tcctctatgc cgaaaacggg
     6601 agcgatgaag ttcttctgaa gtattgttac atcgaggcgg tcaacagggt cgttctcgtc
     6661 aaaggtgccg tccttggctc tcagctcata ccacagcccg tcaagataca gcccgaaggt
     6721 gtgcttttcg gagggtcgga aattctccgc gcccttttcg gttacataga aattcttttc
     6781 ggagagccta ataatctcct cggcgctgcg gttttcgctg tctttaagga gtctgttgta
     6841 atccatgagt ttaagatctt cgcacttgaa cactaccgca aggaagaagt tgaactcctc
     6901 ctcgcccgta tagctgccca gctcttttct gcgttttagt cccacatgaa ccgcggaggc
     6961 ggagcggtgg tgaccgtccg ctatataaag gctgtttatc ttcttaaact cctcaattat
     7021 tctattgata tccgtttcgt cgcttacaat ccagactatc tgccttacgc cctggcagga
     7081 tacgaaatcg tactcgggct cggtcttttc gtactttgcg atgatttcat caattgagcc
     7141 gccgcccttg taggttatga aaatcgggcc ggtatgagcg tcgcaggtgt ccacatggcg
     7201 gaacctgtca acctctttgt cagctcttgt aagctcatgc ttcttaataa cattgttcac
     7261 atagtcgtca atcgataggc agccgacaat gcccgtctgg gctctgccgt ccataatctg
     7321 cctgtatata taaaggcagg gcttttcgtc ctgaatgaaa atcccatcgt ttatatagct
     7381 ttcaaggttc tccttagcct ttaaataaac ctcgtcagag tacaggtccg tgccttcggg
     7441 gaggtctacc tcagccttgt cgattctcaa aaaggagatg ggattttgct ttactatttc
     7501 ccttgcctct ttggtgctga gtacatcgta agggagggcg gcaaccgccg cggccttgtc
     7561 tttggtgggt cttattgctt tgaatgctct taaaactgcc atgataactc cttatttctt
     7621 gtctgtagct ttgataaagt aaacatcgtc aatcgacggg taaaaatata ttccgctgac
     7681 ggctttgtga accgccagga atctgctttg gctgaatacg gggagaacga ccgcctcgtc
     7741 tgtgaggatt tgcgcaagtt tctcggcgtt tgacttgaaa tccccctcaa agccgtccga
     7801 aagcagtctc tccacgccgt taataaaggc cttattgaag cagtatatgc cgtcctcgct
     7861 ctcaacggca aagcttctta aataggagga tatatccgcc gtgtcgggct gaaggggata
     7921 gagagcggct tcataattgc ccgactgtac cctctttaca agttccgccc tctcaagggg
     7981 aatgagggat acggaaaagt ttacgccgaa aatcttctgc cattcctgaa gctgaaattt
     8041 cagcatctcc tcgtgctctt taatgcacag aacggaaaag ccgctaagta ccgcgccgtc
     8101 tttctcagcg tccgcctgag cctttttgaa aagagccctt gcctcctcct cgttaaagct
     8161 gaggagagcg gggtttttaa gcccctccgc ctcggcgata gacacgggaa cgggggagag
     8221 agcctttcct ttcatgtcct cgggcacggg gaatgaatct aagttggtgg aaaggacgaa
     8281 agcctttctc atttccttta cgctgaaagg cgctttagcg gagttgaaca caaacgccca
     8341 gagtatgtcc gtcttttccg tcagctttaa attcttgggt ttcttgatgt tccttgcgtc
     8401 agcggcggtt attattcccg cggagtagtc gccgtctgtg accttttcca agacagaggc
     8461 tgtgtcgggg ttaacaaaca gccttaccac atcgggcaca acggtgcgct cgccgctgta
     8521 aacggtgttg cgggtcagtc tcatggagcc gccgggattc catctgaaca gaataaatgg
     8581 gccgttgcag ataatgtatt taagggaaag accgtacctg ccgccgcatg cgttgaaaaa
     8641 tacctcgctg caaggggaga acacagggtc ggtcagcacc cttaaaaaat ccctctccgg
     8701 gtgaatgagg gtgattctaa gctcggtatc agatacagcc tcaacgccaa gctcctctgc
     8761 ggccttttcg ccgtttagga tttgggcggc gttcttaatg gataagaaca gctcagcctt
     8821 cgggcaggcg gtttcgggag tgacggctct gcggaacgcg aaaacaaagt catgggcggt
     8881 gaccctcgtg tcaaagtttt cgggcaggcg gtcggcgaga gcctgtttcg ccgtatttgt
     8941 cagataccac cttgcgctct ctctgatttt aaaggtatat gtaagcccgt cgtcggaaac
     9001 ggtgcagctt tcggccgctc cgggtatgat tttgccgtcg gaatcaacgg tcacaagccc
     9061 ctcaaaggcg ttgcgggcga aaatccgctc gctgaaaaga cgggctatct gcgggtcaag
     9121 gctttttgcc tcctcgggca cgggaaagct aaggtccagc tccttattgg gcagggagca
     9181 gccggataaa gaaacggcaa gtaaagtgag tataagcaaa aggcattgga atctttttct
     9241 cactgtcacc caccccgcat gctgaatttc aaacaaaggt attataacaa aattacattg
     9301 tcttttctac acaaatataa acttattttg gcgttttaac acattccgca cacaagaatg
     9361 caaaaaatga acggacgcat gaaacccttt gcggcagctt taccaatata tgcttgcatt
     9421 tccacatctc aaagtgctaa actacacttt aaaggaggat gcttaatgaa gtttgatttc
     9481 acaggtttgt ctatcgctgc cgtcaccgag gaggagaaga aggcggctga aatattaaag
     9541 gatgaaataa atacaaggac aggccatgcc cccgccgttt cggaaaaggc agagggcaaa
     9601 gccgttgtgt ttaaaaccga taatgaggga gaggttcccc ataaggattc atataaactg
     9661 agcctttgcg gcgagaccct ttatatcatc gcaaagggca taagaggctt tattttcggc
     9721 ataggctatt tcctcagaaa aactgagtac aaaaacgaga agattactct cattaaggat
     9781 atatctggtc tttatgtccc cgacaagaga atcaggggtc atcagcttgg ctacagaacc
     9841 acgcccaaca cctacgacgc gtggacttat gacgactatg taaggtatta caaggacatg
     9901 atgttcttcg gctgcaatat gtgcgagcat ataccttacg aaaaggcggt ctccaacaga
     9961 aaccacctta tgaagtacga tgaggaggag ttcctcattg aagcctcccg ccttgcggac
    10021 gagcttgatt tggatgtttc catctggttc cccaactgcg aggataccct ggaggaggct
    10081 gttgagagaa gaagggagct tttcaagaag attcccagaa tcaacgccgt attcccgccc
    10141 ggaggagacc ccggcgacct tgaggcagag gagtttgtaa ggcgcgcaat agcattcggc
    10201 aaggtgctca aggagttcca cccgaatgcg ggtatgtggc cgtcggctca gaagcctaag
    10261 aaaatcctca gctggggcga cgactttatt gaggagatgg aaaagctgcc cgatgaaatc
    10321 gagggcatcg taacgggacc caacagggcg ttccccctcc acgagctaag acgcctcctc
    10381 cccaccaaat accccatccg cctttatacc gacatcaccc acaatgtaag gtgcgaatac
    10441 cccgtccact acccctatga cgactggcac tactccttaa cctcgtgcct cagccgcgag
    10501 agcgtaaacc cccgccccgc ggagtacgag atgattcacc gccttacaag gggctatata
    10561 gtcggctccg tcagctactc cgagggagta aacgacgacc ttaacaaaat ggtatggagc
    10621 aaccttgact tcttccccaa tgtttccatc agggagacca tagaggacta cgcaagactc
    10681 ttcttccccg gcgctcccgc cgaccttgtg accgagggtc ttttgggctt tgagcacaac
    10741 tggatgggcg atcccgtcaa caatcccggc atagaggcaa ccttaaaaat ctggcagggt
    10801 cttgcaagcg actatcctaa gctcatggac aactggcgct ttgtaatgca ccttttcaga
    10861 gccgagtgcg acgccattgt ccgcagacgc agaatctttg aattaaacct catcgacgac
    10921 gcgctttatg ttctgaataa agagggtctt gaggcagctc tcaaagtgct tgaggaaacc
    10981 gagtttgacg acgagtacaa atccctcaga gagcatatcg aggtgcttgc cgaaaaactc
    11041 tttaatctca tcggcatgca gcttgatgtg gcccgctacg gcgccaacag ctgggagaga
    11101 ggagcgaccc ttgagaccat cgacctgccc gtcaccgaca gggagtggct catcggcaga
    11161 ttcaaacacg ctctgacact gcccgagagc gaaagagacg gctttgttca cagagtgatt
    11221 aacaggaaca aggtaggtcc cgaggagttc tactactcct ttgcggagca cggctttgat
    11281 atgctgggcg tcagacaaga gggagaagta tatatcaact tccagggcga cagacccgaa
    11341 aaccgcggca aaatgcccat gtccatgctt aaggttttcg acaacctgac cttcaggtgc
    11401 aagctgggcg gctttgccga gaatacggac tataagctca gaatcacctt caagtccgac
    11461 aagcacccga ttttcaaccg tcactatata attgcaaacg gcgttgaaat ctacaaggga
    11521 ccccagttcg gaggcgaaag ggacgagaag ttcgacgagg agctgcttgc ccccggcttt
    11581 gaaacagcaa cctacctgct gcccgcctcg gtattcaaaa acggcaccct tgagctggaa
    11641 ttcggcgagc ccttaatggg agtcatgatg tgcgaattct ggattaccaa ggcataaaga
    11701 aggctcaatt gccataaggc ttaaactata aatttgattt cagggggata aaacaatgaa
    11761 gaaaaagacg gctgcgatgc tttccgccgc agcttttgcc gcaggcagcg cgctgaccct
    11821 aaagcttcag caggagctta aaaaggacga aaacaagcgt gtgctttacg acaagggcaa
    11881 caaaacattc tacggcgcct taaagggcat agcctcagtt ctgcccgagc cgccgatggt
    11941 ccatttggtt gactatgtaa gcgaaaattt ctataaaggt cacgatgaat tcatcgacaa
    12001 gccgaagaaa ggcgcgaaat ggcgtctggg ctttgcctgc gacagcataa ttcccgacga
    12061 tatctccacc ggagaatatt atatgggcgg ctatctcagc atccccgcga acagggtgga
    12121 gggagtgctt gacggactta tgttccgctg catagccgtt gacgacggaa acggcagagg
    12181 caccgtggtt ttcggcgtta tagattccat aggcctcagc gccggcgata taagggaaat
    12241 cagaaaaaga ctttccgact tcgccaaaga gcacaacatc atcagcatca acataagcgc
    12301 gagccactgc cattccggca ttgacaccca gggtctttgg ggcaatctca aagagatttt
    12361 ccccaccaac tacaaggcgg taaagaacag ggagttccac aagctcaaga gcggcaagaa
    12421 tcccaagcac atggaaatga tattcaatat cgccgtggat tccatcaaga gagccgtaga
    12481 gagcatggag cccggcaccc tttacagcgc caccgtcccc ggcagaggct acgcaaggga
    12541 caagagaccg cccaatgtgg ttgtcgacga aatcaccacc ttcaagttcg tgcccgacga
    12601 caaggacaag aggcccacaa ggctggttct catggcagcg catcccacat ccttcccgga
    12661 caagaacaag atggtcagcg gcgactatcc ctatcacatc tgcaacaagc tgcataaact
    12721 gggagagaac gctgtattct tccagggtcc gcaggcttcc atcgccagca acaagtcggc
    12781 gttcacagcc tccgactcca aagaggacga cccgccctac aagaagatgg gcgagggcgt
    12841 agcggaatac ctctacaacc tgcccgaaga cgagtatgag gaagtaaagc ccatactcaa
    12901 catccgccac agagaggtag tcctgcccgt caccaactat ctgtatcaca tccttggaaa
    12961 attgatgata gtaaccacca taatggtaaa gacatcgggc agaaaggacg acctggcggt
    13021 cattaccgag ataggctacg cagaggtggg cgacaaaata aagtttgccc tcattcccgg
    13081 cgagcttatg cctgaaatct tgcttggcgg cttctactct catgaagagg catacaccag
    13141 aaccgactgg gcaatgccga gccttaaaga tattgcgggc gaaaagctta tcggcatagg
    13201 actttgcaac gacgccatcg gctacatagt ccccgataac gactacggca gcattttcgc
    13261 tccctatcat tatgaggaat cggtatccgt cggcgaaaaa accgcttcga ccatagtcac
    13321 cgaatttatg aaaatggttg aagaagtcag gggttaattc gtcatttgtc caaaagccgg
    13381 tgaatttatg ctaatatagc aaaaggaacc agctttaggt tattaaatac agagcttctt
    13441 tccccgggaa ctttaaagca accgggggaa attttgcata tttttttcgg gggaattttt
    13501 gcaatgaaat acctttacga aatgcacctg cacacttcag aggtcagcgg ctgcggcaga
    13561 gtttcgggtg cggactccgc agtattctat aaagaaaagg gctatgcggg cataatcgtc
    13621 accgaccata tggtgcccag agccttccgg ggcatggagg acgcctcatg ggacgaaaag
    13681 gtggaccgct ttatggaagg ctacaaaaaa gcggttcagg ccgcgaagga cgaaaacttt
    13741 gatgtcatcc tcagcatgga gcttcgcttt atgagcgaga gcgagaacga ctatcttatc
    13801 tacggcttta cagaggagtg gctgagaaac aatcccgagc cctacaacct gaccttaaag
    13861 cagtttaaga aaatagcgga tgagaacggt ttcctgattt atcaggctca ccccttcaga
    13921 gaccacatga ccgtagtaga tcccaagtac ctgcacggta tcgaggtgca taacggtcac
    13981 cacggtcacg actcaagaaa cccgattgcc gaggcatggg cggagctcca cggactgccc
    14041 cagtcctcag gctccgacta tcactatctt gagcaccttg caaggggcgg aatgctcttt
    14101 tatgagagaa tcaggactcc ccaggacctt gtaaagcagc ttaaaagcgg acgctacgga
    14161 cttttcaagg gcggatttta ggtcataaga cacccggcgt gttaatttag atttaaggag
    14221 gcatttatga aaaactttag gcgagcagta tcattactct tggttctcac agtactttgc
    14281 ttcagcgttt cattcgctgc gtttgccgag ggcccggaaa acgagggcgg cctgcttacc
    14341 gtcatgtcct acaatgtatc gggacttccc tttgcgggcg cggtacaggg ctcggaaatc
    14401 gtgcccggct atcccaaggc agagcttttg ggcgcgctta tgaatcaggt tgatgcggac
    14461 atcatcggcg ttcaagagga ttttaacttc catgattctc ttgccgccaa gatgaccaac
    14521 ttcccgtata agactttctc cagcggcccc attcctcttg gcgacggtct caatatattc
    14581 tcaaagctgc ctatctacaa tatctcaagg cacacatggg aaaagagata cggagtattc
    14641 aagggcgcgg cggaccagct cactcccaag ggattcctct attccgtggt tgaaattgca
    14701 gagggcgttt acattgactt ctatgtcctc cacgctgacg ccggcggcga cccccaatcc
    14761 actgccgcaa gaatagacaa ctacaggcag cttaccgagc acattaacac tagaccccac
    14821 gacagagccg taatagttgt cggcgacttt aacgcgagga tactctccgg ctttgaaacc
    14881 gatatgctca ataaccttat cttccccgcg ggtctttccg attcatgggt agagctttgc
    14941 aacaacggcg tttacagcac cgagcctcag tttgaggagt acgaggtatt cgagtccata
    15001 gataaggtta tgtacaaaaa cggcggcggc gttgagttca cccccgttga cattgagcac
    15061 tacaagctca agaacgagag aggcggtaca tacactgacc atcacgcaac aaaggttgtt
    15121 ctcagctaca ccgtaaacga gatagttccc atgaacgaag agctggcaga gcccacaccc
    15181 cacgacacag gctttatggc aatgttcaaa gccgtcctca cggcattaaa gcttctcatc
    15241 agcgaccttg gaaatctgtt ctaaacaaaa gcccgacctt gagtcgggtt tattttttgt
    15301 ttttaaaaag ctttttgttg tatctttctt cctcgctgaa tacgcagccg cagtatttct
    15361 gcatataaat gcccatctct ctggctctct gctgaccttc tctgaaatag ggtctgaaat
    15421 ccctgtaaag aaaccgaacg ccgtattttt cgcccgtttt ctcaaaaact tcttttaaaa
    15481 gctcgtgatt ttgatatggg cttataagca aagtcgtggt atacgcttca aagccgttat
    15541 cggcggcgta ttccgccgct ttttccgccc tcgtttcgta gcagtaaacg cagcgggaat
    15601 caaattcggg cgttacatta atgacaaatt ctcttaaatt gtagtaatcc tctaagacaa
    15661 gctcaagccc tgtcttttct gcgtattcct ttaaagcctc gcgcctttgc ttatattctg
    15721 taaagggatg aatatttgga ttgaaccaat atccgacggg ttcaattccc tcattcctca
    15781 gcgtgtcaac gcacataaca gagcagggcg cgcagcaaat atgaagcagt gttttcattt
    15841 ttatcatgcc ttcaattttt tcttgttata acttaaattt ctatgaatta aaaataatat
    15901 acggtcaaaa ataatagcgg acaccgaaat caagcaaaaa ttctcgtgcg tgtctgtttg
    15961 attgatatag atttgtgatt tgatatagat ttagctgtac acttgtaaag ggaaggaaag
    16021 aatttttgcg atgttccgca aagcggtctg gtgccgcgtg cggcggtgtc caaaacaatt
    16081 taatatagct tggattgggg ctattgctcc gctgaaatcc aagtgaggaa tgacatctta
    16141 attgatagct tccaaagaag tctgcggaga tttccgcaga cttcgagttt tttacatata
    16201 tgtaattctg tccttgtatt tttcgaacat tctggcgttg tactcatcgc cgccgtctaa
    16261 gttcgcgctt ctgaaaacag gcgggtcaat gcctcttttt aaaaactccg ccacggactc
    16321 cgcacaaagg gagtttataa tcgccgcgcc cataattgtg gaggttggag cggttttttc
    16381 cgcaaagccg tctattgtga gcaccgcgtc gcctatctcg cccacattgt caatcaccat
    16441 gtcgcagacc tcaaagagcc ttttgccgct ctcatggcgt gagctttgag ccgacgagta
    16501 tttgacgctt gtgattgcga ccgtgtaaat gccccttttc cttgcctcaa tcgccatctc
    16561 aacgggaacg ccgtttctgc ctgaaaccga gtgaatcaca agcaaatctc cctttgaaat
    16621 gggaacggaa ttaagtatct catgggcgta ttcggatttt ctttcaagca cggaggtatc
    16681 cgttacggga acggtgtcaa gggtcagacc ctttggcagt atgggattcg caattacaag
    16741 accgcccgtt ctgtaaaagg cttcctgagc gagaattccc gcgtgggagc agccgaagaa
    16801 gaataccttt ccgccgcttt ccaccgtgtc aacaagctta atagccagcg cctttatgtt
    16861 ttcttcctgg gttttaagga gttcgtctag tttttggtgc agaatctcaa aatactttcc
    16921 ggtataagac ataaggcccc tccaaatatt ttcgaaaagg gtagtccccc aaatttactg
    16981 tttaattccc gccgaaaagg gtaatctcac cgtccgcggc gccgttatcg gttacaaggt
    17041 ttacctgcgg cagagtggtg gtgggtccgg aaggctttgt ggtcgtggtg ggttttgttg
    17101 ttgtggcaga gcctttggtg gtaagctcaa attccttatc cagttttgcc accattctga
    17161 gaatcagcct tgcgtcagcg gcggttacct tgccgttttg gtccacatcc gctataaatt
    17221 cctgctcttt tgagagcgtt tgcagctttg cgacatgcct tagcacaagc cttgcgtcgg
    17281 atgcctgaac cttgccgttt ttgtccacat cgcccgcaag cactgtggag gcgttaagca
    17341 aaaaggcaaa gacaattgac acgattataa gcgaagccgc aaagagctta attgagtttt
    17401 tcattctttt ctcctccgca aaaatgccac ttcctttttt tccattgtag cacatagcaa
    17461 aaaggagaac aagaccgaaa aaacgcttaa aacttagaaa atttaagcaa tcagcggctt
    17521 tctcgcccat gcggaaaaaa tgccgaaagg gagcgccgac ggaaattccg ccccgctttt
    17581 ttctgctgcg ccccggctcg aaacggaaaa accgcctcaa gcacaaaccg ccccataggg
    17641 gcggctgtat ttattttaat tccgctatcg ttactccgtc ctcgccctcg ccaaaggtgc
    17701 caaggcggaa tgtctttatc tgcgggtgct ttcttaaatg ctgatgaaca gcggctctga
    17761 gttttcccgt gcctttgccg tggattatgg taatctcatg gagtcccgct ataacaaggg
    17821 agtcaatcgc caaatccact actggcagag cctcgtccac agtcatgccg cgaatgtcgc
    17881 agcgggtatc ctgagaagcg agcaccttat gctccgactt tacgctctta atcgtgttgt
    17941 atgtgctctg ggcgctcttt tccttcttag ccttgccggc gataagtctt agtctttcct
    18001 tggcgacgcg catcttaatg ccgccgatct ggacctcggc gttctcgctc ttttcgtcat
    18061 agcttgaaac aacgccctcg cagcccatgt ccacaagcag cactctgtcg ccgattttaa
    18121 gagtttcggg cggctcgtca aaggcggcct cggagcgggc gctcattgaa acgggattta
    18181 aggagtcctc aagggcgttt atgctgcttc tgagctttcg cttcagctcc gccgcgccgc
    18241 cgtgctgctt tatttcctcg ctctcttttc tgagcctttc aagctcagcc ataaagtcgg
    18301 cggcctctct ctttgccctg tcggcaaggc gtattgcctc ggtccttgcc ttttcaagct
    18361 ccttttcgct ttgctgaata gccttttcca gcctctcttt agcctcactg agaagtctct
    18421 ccgcctctct cttttcctca agagcctgcc ttaatttctt ctcagcctca aaacgctttt
    18481 cctcaagggc ggtgacaagg ctttcgaact taatgttctc gccggagact aagctctttg
    18541 ccttttcggt tatctccatg ggcagtccca gcttttcgga gatggcaaag gcgttggagc
    18601 gtccgggtat gcccgtcaaa agcctgtatg tgggcttgag tgtatttaca tcaaactcgc
    18661 aggaggcgtt ttccaccctc tcggtctgca gagcataagc cttcagctcc gcatagtggg
    18721 tggtggcggc tattttcgct cccttccgcc ttatatgctc cagaatcgca atcgcaaggg
    18781 cggcgccctc tacggggtcg gtgccggcgc ccaattcgtc tatgagcaca aggctgccct
    18841 cggagcattc ctcaattatc tttacaatgt tcgtcatgtg ggaggagaag gttgaaaggg
    18901 actgctcaat gctctgctcg tcgccgatgt ccgcaaagac gcccttaaag acgcagacct
    18961 tgctgttttc cgacgcgggg atgaaaagcc cgcacatggc catgagcgta agaagtccca
    19021 gcgtcttaat ggaaaccgtc ttgccgcccg tattcggacc cgtaattata agggcgtcat
    19081 agtcaaagcc gagctttaag tcaatgggca caactttgtc ctgggggata agcgggtgcc
    19141 ttgccctttt aagctcaata agccccttgt cgtttaattc gggagcggag gccttcatgc
    19201 tgtaggcaag agccgcctta gcgaaaatga gattcagctc agccgcggcc tttgtgctct
    19261 gctttatggg agcggcaaaa ctgcctgctt ctgaagaaag gtaggtgata atcctttcga
    19321 tttcgtccct ttcttcgctt aaaagcattc ttatttcgtt atttgcctca accacggcgg
    19381 agggctctat aaataccgta gctccgctgg atgaggtatc gtgcacaaga cccgccactt
    19441 ctcctctgtg ttcagcctta acgggcacca catatctgcc gttcctttgg gtaacaatcg
    19501 gctcctgcag aatctttctg taatgcgcgg agcggataag gctctcaagc tgctctttta
    19561 tccccgccgt cttcctgcgg attttccttc ttatatccgc aagcgccggg gaggcggtgt
    19621 cggcgatttc atcctcggat ataatcgcct tcgttatgct ctcctccaat gccttgttag
    19681 gaattagctc ggagaaaaga aaatcaaggc tcgacccgcc gccgcagttt ctgcgccatt
    19741 cgcttagggc gcggatgacc ctgagggact cggctatgtc caaaagctcc cttgttgaaa
    19801 gaacgctgcc cgccgcagcc ctcgcaaggg cattgtccac atttttaagc ccgctgaaag
    19861 atggggctga aaaacgggca atcagggcat aggcgtcctt tgtctgctca agaaggaaat
    19921 tcgccctgtc aagccttgtt tcgggcataa tggcgagagc catttcgtaa gcgtcggagc
    19981 aggcggtcag ccccgccagt ttttcgagta tcttatcaag ttccagaacc tttaccgctc
    20041 ttttaaacga gttttccatt taacctcccg tgaaaataag acggctttta aaaaagccgt
    20101 catttgctta ttttgaaaaa tcgttaagta tctttaaagc caaatcgggc acatctgtta
    20161 ttataccgtc atagccccat tctatgaaag tcttgattac ataatccgta ttgatggtcc
    20221 atgcgttgac cttaaggtcg ttcttatggg cctcctctat gaaatcctca tcgactatca
    20281 ggaatacggg atgcagatgt gtcgcgccta cgctcttggc gtagccgatg ggatcgggga
    20341 taacctttgc cgcgatttcg cacacagggt cataaagaac gcccgtcata accttgtcgt
    20401 caagtcgcct tgcctcttta agcacttcct cgctgaagct ggagataagc aggttctcgt
    20461 agcagccgca ctccttggca aggtcgataa cgccctttac tattgacaaa tcgttgttcg
    20521 ctggtttttt gatttcgaca ttgactatct caagaccctg agccacctcg tagaactctc
    20581 tcagagtggg gattttctcc cccgcgaact cgggtgaaaa atatgagccg aaatcgtact
    20641 gccttaattc ctcaagggtc atatcgcaaa ttgcgccggt gccgttggag gtggcatcta
    20701 tcgtggggtt atggcaaacc acaatgaccc cgtcttttgt aagatgaaca tcgttttcaa
    20761 agccgtgagc ccccatggct tttgcctttt taaaagccgc aagcgtattc tgaggagcag
    20821 cctttgatgc tcccctgtga gcgatgattt ttgtcatttg gcaaatctcc ttttggcgtg
    20881 attttctaac attataatat aatttcacca atttgttaat accttgacag gcttagagtt
    20941 tgaacttgtt gttaaatctt cttaattctc ttttgttcga ttgacttata aaaatctcgg
    21001 tgctacacta ttggagatac tcacatagaa aacggcgcgc ctcggcattt tatgtatatt
    21061 ttgcacaaaa tgggagcgct ccgccgcaaa aaaacaaagg gggaagcaaa gtgtttaccg
    21121 tcccaaattt gaagcttaaa atcaacaaga taataggctc aattcttgtt gctattctga
    21181 gtgtgggcta ttacctcgga cttagctttg acattcaggg cgtgcgttat gacaagtcat
    21241 cggttttgac ctattgtttt tatgactcca catataagct cggcaaaaac gagtttatag
    21301 gcaagaacga gacctacaac atccaccttg cgaagaatga gtacgaggca tgccagattg
    21361 ccgttcgctc aaagatgaac agcacccaca gaaaatacac catagagttc tcccagtttg
    21421 tgaacgagaa cggcgatgtt cttccctccg aagtttttga ggagaagtac atagcatgcg
    21481 tcagcgacaa gaactacggc acattccccg acgctcttgt aaaatacgct cccgctcctt
    21541 tcagtctgag cccgcagcag aacaaggttt tctacattca ggttcacgct ggcaaggaca
    21601 cccccgccgg cacatataag gcaacaatca ccgcaagaag cagcggtcac gacaacgaca
    21661 aggttcagtt catagccgag gttaccgcaa ccgtctggaa ctttgagctg cccgacaccc
    21721 cctcctgcga gaccgcgttt ggactcagca aatacaacat tgtaaacgcc tactacgcaa
    21781 acaatgaccc cgagcgcgct caggcactat atgagcagta ttacgaatac cttgtaaaca
    21841 gaaagatatc cccctacaac ctgcccgttg acattctctc agacgaggcg gacgcataca
    21901 tgagcgaccc cagagtcacc tcgttcatta tcccctacta cagcgacgac gctacccttg
    21961 tcgccgctta caacaaggtt cagtccaacc ccgaatgggc taagaagggc tacttctatc
    22021 ccattgacga accctccagc cttgaggact acggcagata caacgctatt gttgaaagac
    22081 ttgacagact ctgccccggc tttaagatgg taacaccctt ctatcaggca agcattaagg
    22141 aaggcggcaa gacctaccac gccacagagc tccagagcgc gagcaagata gtgtgccccc
    22201 ttacctcctg ctttaacaat aagactttct tggagcagat tgacaagcgc agagccgacg
    22261 gctcaaagat ttggtggtat gtatgctgct caccccaggg aagctactgc aatatcttca
    22321 cccactggga aggcattaag ggcagactcc ttctgtggca gcagaagcag ttcaatgttc
    22381 agggtctcct ctattgggac accacctatt ggaaggatgt agtatcaccg tggggcgacg
    22441 ctctcacaac cccgtggacg ggcaacgaca ccttcggcga cggctctctc ctttatcccg
    22501 gtcaggacgg ccccgttgcc tccctcagac ttgaggaaat cgccgacggc attgaggact
    22561 atgagtacct gacaattgcc gaagaacttt tcggcaggga ctatataaac aataagatcg
    22621 ccaaggttac aaacaccctc acagactaca cccttgacga ctccctcctc gcaaaggtac
    22681 gcgttgaaat cggcaacgac ataaacaacg ctatgtcggg caattaagct ttaacaacaa
    22741 aaggcaggct cgtcctgcct tttttattaa ggtaaggaga gcattatgtt tgacagtttc
    22801 aaaagtcttt cgggagcggc taaaggcttg agccaaaacc tgaaagtgat tgcggaatac
    22861 ggctcagtct caaaaatcct tgaggacata aaaacagagg gcggggagat aaaagaaata
    22921 caatttgtcc ttatatcacc ggacgaactc gggaaaaaag ccgccgaaat tttccccgac
    22981 gctcccgctc ccatttcgga cgcttacata atcctttcgg aaggcgataa actgacagcc
    23041 tattcatcca caaaggcggg tctgcacttt gccgccggag ccataaaaag gcatatcctc
    23101 tgcggcggca ttaaagaggg agaaatctac aacactcccg atgtcccctt cagatgcgta
    23161 aagatgtatc tgcccgcaag ggaggacatt cccgtctttt ttgaaatcat cgagctgtgc
    23221 gtcgctttgg gctgcaacgc cgtaatgctg gaaatcggcg gcgccatgga atataagctc
    23281 caccccgaaa tcaacgaagg ctgggtagaa tattgcgata tgttccgtga cgagcccggc
    23341 gccacagaca aattcaaagc tcagttcgat tttcataaaa actccataca ttccgaaaac
    23401 ggcggaggct attttctcac gcaatccgaa gtccgcgccc ttgccgacta ctgcgaggaa
    23461 cggggtctgg aggtcatccc agaggttccc tcactcagcc actgcgatta tctcctcaca
    23521 aggcacaagg agctgggcga gcgtcgggag gacccatggc ccgataccta ctgcccatca
    23581 agaaaagaat cctatgagct tatctttgat gtgctgaccg aggttattga ggttttccgc
    23641 cccaagagaa taaacatcgg tcacgacgag ttctacacat taaagctttg cccgctttgc
    23701 aaagacaagg acggcagcga aatctttgcc gaggatataa ataaaatcca cgcattccta
    23761 aagtcaaagg gcgtgggcac tgtcatgtgg ggcgaaaagc tccttgcctt ttgcgcaagg
    23821 gacggtcagc ccatgggcgg catggaacgc tataaagaga gaccgaacct ttacatcccc
    23881 gaaacctaca aggcaaggga ccttatttca aaggagattg agattctcca ctggtactgg
    23941 ggcgtaaata aggagtgcga ggaccagctc tatgacaggg gctttaccct ttatttcggc
    24001 aacttctccg ccgttgccat ggacgactgg aaaggaaggc tcaaaagggg agtcaagggt
    24061 atttgcatat caaactggag ccgcctggac atcctccata tgcaaaggaa cacagtcctt
    24121 ttagaaatgg cgctttcctc aatgatgatt tggaatccga attacagcga aaagaatttt
    24181 gcggagaata tcatcaaggc gtccgatttg ctctaccgcc tgaagaatta caagaccctg
    24241 aggggaaagc atatcgaaat ccgccatgcg gtaaacttac gccatgagca caaacccttt
    24301 gttgacggca atttcattga ttatgaaaag gactatttgg gcgagtatga gattgtctgc
    24361 aaaaacggcg agaaactgac agtgccgatt tattacgggc taaatatcgg ctgcatggac
    24421 ggcagatggg tgatgacacc gtcaagcagg gcaaggtatg tgggcaacga gtttgacacc
    24481 cacattctcg agcctgccta cacctgccgc atagaaaggg acggcggcaa aaccttctat
    24541 gtaattgcgg tgagcgttaa cgacgatgtt gagagcataa aactgaacac acaccttgac
    24601 ccaagtttct ttgaatggca agtgctttaa tgccaagcca aaccaaaacc gagagccgcg
    24661 cgcccgcgcc gccctcggtt ttttgtttct attatatata tatatatata tatgtatccc
    24721 cgccaaacaa ccgaagggac tttaatcagt cctccgaatc cccgccgccc cgccgaggtc
    24781 gccccgcccg caaggagcag acatttcgca aagagagtag ccgccgtcag tgccaccgcc
    24841 cccgcccacg gggatttccc gtaaccgctg ctccatccgc cgagtgtcac caagacacag
    24901 ggggtactgc aagccgccac cgccgagggg ccttcattcc ttcctccgaa tccccgcccc
    24961 tccgccgagg tcgcccgccg ccggaaagaa atagcccgca agctgacaac gcccgcccgc
    25021 taagtgtcgc cccgccgcgg cccagctggc acagaaaagg ggcgatgacg caaagaaaaa
    25081 gcagcgcccc tgtatttccc gcttgatatt atgcgagaag gggcatataa taaaagccgt
    25141 tatgcgccgg cggctttagt tttttctcgc ttaaaaaacg cccggatttt ctaaatttcg
    25201 atacattttt cgcattaaag ctcatataat atgcttaact tgcaaaaatt attattaaat
    25261 tagaataaat tttaaaattc tacttgcatt gagctttgtt ttgtgatatg attttcgagc
    25321 gtgtaaaagg gctttgtata taaagctctt ttatacagta attaaggcga tgatgcggaa
    25381 ggtggctgct ctttgaagca gggaatttcc gcggagtatg tccgattcga aaaccgggcg
    25441 atctaagggt ctttttgcgg ttttttgccg aaaaaatgcc ctgaattgag attttagcaa
    25501 cccggaaata ccgagatttc cggctttttt ttacggaagc aagcggaata cccggagcag
    25561 ttgttaaaat ccttgcccgc ctaaactaaa ggaggcaaat ttatggcagt aaaagagaaa
    25621 atcagaatca ggcttaagag ctacgaccac aaccttgttg acaaggcagc agagaaaatc
    25681 gtagaaacag ccaagcgcac aggcgcgaga gtttctggcc ccgtgccgct gcccacagag
    25741 aaagaggtta tcaccatcct cagagctgtt cacaagtata aggactccag agagcagttc
    25801 gagcagcgca cacacaagag gctcatcgat attctccgtc cctctaacaa gacggttgag
    25861 gctcttaccg aactccagct tcccgctggc gtggacatcg agattaagct gtaacaggct
    25921 ttttcggcat aatctacggc gcagattaag tctgctctta taaagtgccg cagatcatgt
    25981 tgacaatcag tcaaccaata atctgacagg aggaaattca atgcaaaaag gtcttatagg
    26041 caaaaagatc ggtatgacac agctttttga cgaaaaggga aatgttattc ccgtaactgt
    26101 tatagagctt ggtccctgcc cggtcgtaca gaagaagacc gttgaaaccg acggctatga
    26161 cgcagtacag ctcggtttcg gcgaagtaaa ggttcagaga gtcaacaaac ccgttaaggg
    26221 acatttcgca aaggcaaatg tagctcccag aaagtacctt aaggagttca gacttgacga
    26281 tgtaagctcc ctcaatgttg gcgacatcat caaggcagac atctttgaag tcggcgagaa
    26341 agttgatgta acaggcatca ccaaaggtaa gggaacagcc ggcgtcatta agagatggaa
    26401 cttcagtcgt cttaaggagt cccacggctc cggtcctgtt gtccgcaagg gcggttcaat
    26461 aggctccaac tcagacccct caagagtatt caagggcaag aggatggcag gccgtctcgg
    26521 tcatgaaaag gttacaattc agaatctgga tgttgtcaag gttgacgccg aaaacaatct
    26581 tatcgctgtt aaaggcgcag ttcccggtcc ccgcggcggc atcgtgtttg taactgacaa
    26641 tgtcaagaaa gcgtaaggga ggaaagacag atgccaattg taccagtata taacgcagcc
    26701 ggcgaaaaga ttgacgatat aacccttaag gatgaaatat tcgcaatcga gccgaacaca
    26761 cacgctgttc atttagctgt tgtcagctat cttgcaaatc agcgtcaggg cacccagtcc
    26821 acccttaccc gtgcagaggt aagcggcggc ggcagaaagc cctggagaca gaagggcaca
    26881 ggccgcgcaa ggcatggctc catcagagct ccccagtggc gtcacggcgg cgtagcgctc
    26941 ggccccaagc cccgcgatta tgtaaagcaa gttaacaaga aggttaagag gcttgctctc
    27001 aagtccgttc tgtcggccaa gctcagcgct gacgaaatca aggttcttga cagcttcggc
    27061 cttgaagccg taaagactaa agaagctctc aaggttctca aaaaccttga caccggcaag
    27121 aagactctca tcgttcttcc cgaaaaggac gacatagtct atctcagcac ccgcaacatc
    27181 tgcggcgtta agactgtaac ggtggattca cttaatgtat atgatctggt caactgcaat
    27241 accgttctct tcctcaggaa tgcagttacc gcgttagagg aggtttatgc atgagcaagc
    27301 tcccgcagga tatcatcatc cgccccatca tcacagagga aagcatgaag ggcgcgcctg
    27361 ttaagaagta cgcgttccag gtagccaaag acgccaacaa gatagagata gccaaggctg
    27421 tcgaaaagct cttcggcgtt gaggtcgcaa aggttaacac catgaatgtt aagggtcacc
    27481 ttcgcagata cggcagatac gagggctaca gacccaactg gaagaaggct gtcgtaactc
    27541 ttaccgaaaa gtcaaagtca atcgagttct ttgacggttt actctaattt gatttaagcc
    27601 gaaagtattt ccggtttaat aggcacggcg aagtatggag cttcagccac tccgcgaagc
    27661 cgaagccgca gaaaatatct gtaattattc ctaatatcat cgtttaggaa agaaaggcga
    27721 gtgacataat gtcagttaag gtctataagc cgacatcaaa cggaagacgc aatatgtcgg
    27781 ttactgatta ttcagtgctt tcaaaggtag aacctgaaaa gagcctgctt gagcccctgc
    27841 gcaaaaaatc cggcagaaac agctacggca ggatcacagt ccgtcacaga ggcggcggaa
    27901 accgcagaaa gtaccgtatt attgacttta agagaagcaa gttcggcgta cccgctgttg
    27961 ttcagaccat cgagtacgac cccaaccgca cggcacacat cgctctgatt cagtatgaag
    28021 acggcgagaa gagctatatc ttggctcccg aaggcctcaa agtaggcgac accgtagttg
    28081 cgggtcccga cgcggacatc aaggtaggca acgcacttcc tcttgcaaac atcccggtgg
    28141 gtacattcgt acacaatgtt gagatgcacc ccggcagagg cggccagata gcaagagccg
    28201 caggtcaggc agcgcagctc atggctaaag aaggcaatct tgcacttctg cgcctccctt
    28261 caggcgagct cagaaatgta cctgcggaat gcatagccac aataggcaat gttggtaatc
    28321 aggagcacga aaatgttaag atcggcaagg caggccgcac ccgtcacatg ggtcgccgtc
    28381 ccacagttcg cggttcagcc atgaaccctg ttgaccatcc ccacggcggc ggtgaaggca
    28441 aggctccgat cggaagaccc ggccctgtta ccccttgggg caagcctgct ctcggataca
    28501 agacacgcaa gaagaagaac aagtccaaca agtttattgt taagcgtcgt aaccagattt
    28561 aatcgaggaa aggagcagga tttatgggta gaagcgttaa aaaaggacct tatgtcgata
    28621 aaaagctgtt agctaaagtc agacagatga acgaaaaggg cgagaaaatc gttatcaaga
    28681 cctggagccg cagctctact attttccccg attttgtagg ccataccttt gcagttcatg
    28741 acggtcgcaa gcatgttccc gtttatgtta cggaggatat ggtaggtcat aaactcggag
    28801 agtttgcacc aacccgcaca ttccgcggac acgcaggctc caagacatcc aacacaggca
    28861 aataatcggt caaaaggagt gtaaactatg gaagcaaccg caaaggcaac ttttcttcgc
    28921 gtagcgccga gaaagattca gatagttctc gaccttattc gtaataagtc tgccgaagaa
    28981 gctttagcaa tcctcaaata cacgcccaag gccgcgtgtg agccactgct taagctcctt
    29041 aagtccgcaa ttgcaaatgc ggaaaacaat catgatatga atgtagcatc cctttatgta
    29101 gctgagtgct atgtgagcca gggtcccacg ctcaagcgca taagacccag agctcagggc
    29161 cgtgcttaca ggataaacaa gaagacctcg cacgtgacac tggtgctcaa agagagagaa
    29221 taaagggagg aggcaaagat aatgggacag aaagttaatc ccaccggtct gagaataggc
    29281 gttataaagg actgggagtc aaagtggtac gccgacaaga aaaccttcag caacaatctc
    29341 atcgccgact ataagctcag agagtatctc tatgagaccc tcaaggcagc aggcgttccc
    29401 aagattgaga tagagcgcga cacaagaaga gttcgtgtta atatacactg cgcaaagccc
    29461 ggcatcgtta tcggcaaggg cggagctgaa atcgaaaagc tcaaggcaca gtgcaaaaag
    29521 attctcggaa ccgatgaagt tataatcaac atcgtagaag ttaagcagcc tgaccttaac
    29581 gctcagcttg tagcagaaaa catcgctgcc cagcttgaaa gaagaatttc cttccgtcgt
    29641 gccatgaagc aggctatcgg ccgcacaatg cgtctcggcg caaagggcat taagactcag
    29701 gtaagcggac gcctcggcgg cgcggaaatc gcccgtacag agcattatca cgaaggcaca
    29761 attccgcttc agacagtcag agccgacatc gactacggct ttgccgaagc acataccaca
    29821 tacggccgca taggcgttaa ggtttggatt tatcaaggcg aggtattcca cgatacccgc
    29881 agcaacagaa acaagaggga agggagagct ggccatgctg ctaccaaagc gcgttaaata
    29941 tcgcagagta cagagaggac gccttaaggg tgcggcaagc agaggtacaa aaatcacata
    30001 cggtgatttc ggtcttgtag ctcttgagcc cgcatggatt acctcaaatc agattgaggc
    30061 agcccgtgtc gcaatgaccc gttatatcag gcgtggcggt caggtttgga taaagatatt
    30121 ccctgacaag ccgattacag aaaagcccgc cgagacccga atgggttccg gtaaaggttc
    30181 acccgaatac tgggtagccg ttgttaagcc cggccgtgtt atgttcgaaa tcggcggcgt
    30241 aagcgaggag cttgccagag aggctatgcg ccttgcagca cacaaactgc ctatcaagtg
    30301 caagtttgtt accaagaagg aacaggatgg tgagcaggaa tgaaagcatc cgaattcaga
    30361 aagatgacag cccaggagct ggatgctaag ctccttgagc tgaaggacga actttttaaa
    30421 ttgcgtttcc agcaaaccat caaccagctt gaaaacccca tgcgcatcag ggccgtgaaa
    30481 aaggacatcg cacgcttgct gacagtaaag cgcgacaaag agcttcatgg ctctgaatcg
    30541 taagacaagg gaggaaagcg ctaatggaaa gaaatttaag aaaaacccgt gtcggcgtcg
    30601 ttgcaagcga taagatggat aagacagttg ttgtagctgt taaagaccgt gttaagcacc
    30661 ctctttacaa aaagattgtc aaccgcacca ttagacttaa ggctcatgac gagaacaacg
    30721 agtgccgcgt aggcgacaga gtattgatta tggaaacccg tccgctctcc aaggagaaga
    30781 gatggcgtgt cgccgaaatt atagagaaag ccaagtaagg aggcagacaa agtgattcag
    30841 caacaaacac ttctcaaagt cgccgacaat acaggcgcaa aagagattat gtgcatcaga
    30901 gtgctcggcg gttccggcag gaaatacgcc aacatcggag atgttattgt cgcttctgtt
    30961 aaaaaggcta cgcccggcgg cgttgttaaa aaaggtgatg tggtaaaggc cgttgttgtc
    31021 agaagtgcct ttggcgttca cagaaacgat ggaacttaca ttcgctttga tgaaaacgca
    31081 gctgttatta tcagagatga caagaacccg agaggcaccc gtatttttgg accggttgca
    31141 agagaactga gagacaaaga ctataccaag attctcagcc ttgctcccga agtgctttga
    31201 tgggaggtcg tagatatgaa gaagaaggtt catgttaaga ccggggatac ggttttggtt
    31261 ataaaaggca aggaccgcgg caagaccggc aaggttcttg aggtcagccc cgaagagggc
    31321 aaggttattg ttgaaggcat caacatcgta accaagcatg ttaaaccccg cagaatgggc
    31381 gagcccggcg gccttatcaa ggctgaagct gccctctatg ccgataaggt tcagcttgtt
    31441 tgccccaagt gcggcaagcc caccagagtc ggccatgcat ttgactcaga aggcaaaaaa
    31501 gtcagagtat gcaagaagag cggctgtgct gctcagtttg aataaggagg agcggacaga
    31561 gaatgcctaa gatgcccaga ttaaaagaat tatatttaaa cgaagtcgct cccgcactta
    31621 tgaagaagtt caattacaag agcgtcatgc agattccgaa gctcgacaag gtagttatca
    31681 atgtcggctg cggtgaagca agggataatc cgaaggtcct cgaccatgta gtatccgaca
    31741 tagcacagat tacaggccag aagcctgttg tggtaaaggc taagaagtct gttgccaact
    31801 tcaaaatccg tgcgggcatg cctaacggcg ttaaggttac actccgtggc gacaggatgt
    31861 acgagttcct tgacagactg ttcaaccttg cgcttcccag agtgcgcgac tttagaggaa
    31921 taaatccgaa ttcattcgac ggcagaggaa actatgccct cggtgttaaa gagcagctta
    31981 tcttcccgga aatcgattac gacaagatcg ataaggttcg cggcatggat atcgcaatag
    32041 taaccacagc aaaaactgat gaagaggccc gtgagcttct ggctcttatg ggcgcaccgt
    32101 ttacaagata agggggaggg ttttaaatgg caaagacatc tatgatagtc aggtctcaga
    32161 gaccggcgaa gtattcaacc aggcagcaca acagatgcaa gatttgcgga agaccccacg
    32221 cttatctccg taaatatggt gtttgtcgta tatgcttcag ggaacttgcc cataagggtc
    32281 aaatccccgg agtcaagaaa gcaagctggt aaacagctat tgagcaaagg aggttaacct
    32341 gatgcagatt accgattcca tcgccgatat gctcacaaga atcagaaacg ctacatcggc
    32401 aaagcatgac acagtgagcg tcccggcgtc aaacatcaag aaggcgatag ctcaaattct
    32461 tcttgacgag ggatatatta aaggctttaa ggtaattgac gacggtaagc agggcgtaat
    32521 cgaaatcgct ctgaagtacg gtcccaacaa aacaagcgtt attaccggac tcagaagagt
    32581 atctaagccc ggacttcgta tatacacaaa ttgcgagaat atgcccaagg ttatgagagg
    32641 acttggcata gccataatct ctacatcaaa gggtgttatg actgataagg acgcacgcaa
    32701 gcttaatgtc ggcggcgaag tccttgcatt tatctggtaa ggaggactaa gggatgtcac
    32761 gtataggcaa aaagccgatt cctgttccgg ccggcgtcga agttaacatt gccggcaatg
    32821 tagtgactgt aaagggtccc aagggcactc ttactgtaaa tgctcacagg aacatgaagg
    32881 tagcccttga gaacggtgaa attattgtta cccgccccaa tgatgataaa gagaacagag
    32941 ccctccatgg ccttacccgt tctcttatcg caaacatggt agagggcgtt acaaacggat
    33001 ttaagaaaga gctcgaaatc aacggcgtag gatacagagc agctgcccag ggcggcaaga
    33061 agataaccct caatgtgggc tattcccacc ccgttgactt ggaggctccc gcaggcgtta
    33121 cagtagaaac tcctgctccc aacaaaatag ttattaccgg tgcggataag caggctgtcg
    33181 gcgagttcgc agcccaggtc agaaaggttc gtccgcctga gccctatcac ggtaagggca
    33241 tcaagtattc cgacgaggta attcgccgca aggaaggcaa ggccggtaag ggcggaaagt
    33301 aaaggagtga aggcaaatgg taaacagacc agattccaat aaagcaagaa ttaagcgtca
    33361 caagagagta cgcgcaaagg ttaaaggcac acccgagtgc ccgaggctta atgttttccg
    33421 ttcactcaag catatttacg cccagcttat agacgatgtt aacggtgtaa cacttgtttc
    33481 agcgtcaacc gtcgaaaagg gctttgaggg ttacggcgga aatgttgaag ccgcaagaaa
    33541 agtaggcaaa ctcattgcag agagagcagc ggaaaagaac attgaaaagg ttgtttttga
    33601 ccgcggcggt tatatctatc acggccgtgt taagagcttg gccgaaggcg cccgcgaggg
    33661 tggactgaag ttctaagaag aggaggaata cttttggcta agtttgaagc gcaggaaaag
    33721 tcagaattca aagagcgctt agtcgctatt aaccgcgttg ccaagacggt taagggcggt
    33781 cgcattttca agttctctgc tcttgttgtt gtcggcgatg aaaacggcac cgtaggcttc
    33841 ggtctcggca aatcgggcga agttccggat gctatccgca aggcaataga agacgcaaag
    33901 aagaacctta tcaaggtttc cctcaagggt acaacaattc ctcacgaggt tatcggcaaa
    33961 ttcggctccg gcgtagtgct tatgaagccc gccgcacccg gtaccggcgt tattgccggc
    34021 ggcgcagtcc gtgccgtagt tgaaacagtt ggcattaaag acatccgcac aaaggcatta
    34081 aggtcaagca atccctgcaa tgttgtcagg gcaacgattg atggcttaaa gcagctcagg
    34141 aatgccgacg aggttgccgc tataaggggc aagaaagtag aagagattat aggttaagga
    34201 gcgagaagaa tggcaaacat taaggttaag ttagtcagga gccttatcgg ttccaccaag
    34261 ccgcagattg ccaccgctca cgcccttggt cttagaaaag taggcgatga gtcggtacag
    34321 cccgacaatc cccagactcg cggcaaaata aacaagataa atcatcttgt tgaaatcgcg
    34381 gatgcataag aaaggatgga taacatgaaa ctgcacgagt tatcaccggc tcccggatca
    34441 acaaaagatg caaaaagagt aggcaggggc cacggctcgg gccacggcaa gacctccggc
    34501 aagggtcata agggtcagaa ggcaagagcg ggcaagggtc cccgcgtcgg ctttgaaggc
    34561 ggccagatgc cccttcagag acgagttccg aagaggggct ttaacaatat cttcgcaaca
    34621 aaattcgctg ttgtaaacct tgcagatctg gagagagttt tctccgacgg tcaggttgta
    34681 gatatcgagg cattaagaca ggccggactt ataaagaagc ctcttgacgg cgttaaggtt
    34741 cttggtaacg gagagttatc aaagaagctg acagttaagg ttaacgccta ttccgaaacc
    34801 gcgaaagcaa agatagaggc tgccggcggc acggcagaaa tcatttagca caactgcaaa
    34861 ggcagggtgc ttccgattgg aaggagcgtg ttaaccgatg ttcaaaacca ttgctaatgc
    34921 ctggagaatt gaggacattc gcaagaagct cctttatacg gcgcttattc tcttcatctt
    34981 caggataggt gcggcaatcc ccgttccctt tgttaacgct gcggagggta ttatcagcaa
    35041 taccgatact tcctcattca tcagctacct tatcatgatg accggcgatg cgtttaacta
    35101 cggtaccatt ttcgcaatgt ccattactcc gtacattaac gcgtcaatta tcctgcagct
    35161 cttgcaggtt gttataccgg ctcttgagcg tcttgctcag gaaggcgagg aaggcagaaa
    35221 gaagatttcc gccatcacaa gatatactgc cgtcggtctc ggacttctcc agagcatcgc
    35281 ttactatgta tatctcagaa gcggcggttt ccttaccaca gacgatgcgg gcaagaagtt
    35341 cgcaggtttc cccgcggttc ttcaggctct cgtaattatc ggtatcctca ccgcaggctc
    35401 cgcgcttgtt atgtggctcg gtgagcagat taacgaaaag ggaataggca acggcatttc
    35461 gctcatcctt tttacgggca tcatttcaag aatccccacg ctggtaacaa cactgttcca
    35521 gtactttaac agaggcggcg ttttctacgg tctcgcaccg cttgccgcag tcatcatgat
    35581 tcttatggtt gccttcatcg tctggatgga taatgcggag caccgcatac ccgttcagta
    35641 cgcgaaaagg gttgtaggcc gcaagatgta cggcggccag agctcgcaca tcccgcttaa
    35701 agttaatatg tcaggtgttc ttcctgtcat tttcgcaagc tcaatcctgt ccataccccc
    35761 cacaattcag ctctttgtaa aggctgagaa caagttctgg acaacgattt tgggctggtt
    35821 caaccagaca tcaccgctct atgtagttat ctatttcttc atgattatat tcttcgctta
    35881 cttctattcg cagattcagt ataatccgat agagatggct aacaatctcc gcaagaattc
    35941 cggcgcaatt cccggaattc gtcccggcga gcccaccgcg gcatatatcg ccaagattat
    36001 gtcaaagata accctattgg gcgcgctgct tctgagcgta gtagctctgt tcccgattat
    36061 cttcacacag ctcacaactc tgattttcgg cgaaggcaga accatgaaca tatcgctcgg
    36121 cggcacatcc gtaatcattc tcgttggcgt cgctctcgaa accgtacagc agcttgagag
    36181 ccagatgatg atgcgccact ataagggctt ccttgactaa gttcctaaac ggaggtttca
    36241 aatgagaatt atttttctgg gagctcccgg agcaggaaaa ggaacacagg cggaaacagt
    36301 cagccgaaag ctgaacattc ccacaatatc aacaggcaat atcatccgtg ccgccattaa
    36361 ggatggcaca aagatgggcc ttgccgctaa agaatatacc gacaagggcc tgcttgttcc
    36421 ggacgatgtt gtcataggca taattaagga acgcctctcg gaggatgact gcaaggaagg
    36481 cttcattctt gacggcttcc cccgcaccat tccccaggcg gaaagcctcg accagatggg
    36541 catagagatt gacaaggtta tcaatatcca tgtccctgac gagacgat
//
LOCUS       DAONVQ010000003        35305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_766_length_35305_cov_4.522411, whole genome shotgun sequence.
ACCESSION   DAONVQ010000003 DAONVQ010000000
VERSION     DAONVQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 35305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35305
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_766_length_35305_cov_4.522411"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2810)
                     /gene="rpoB"
                     /locus_tag="PLD98_00495"
     CDS             complement(<1..2810)
                     /gene="rpoB"
                     /locus_tag="PLD98_00495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQA04772.1"
                     /translation="MVNVKPVKRGKRTRMSFGRINEVMEMPNLIEVQKNSYKWFLDVG
                     LSEVFRDIGEITDYSGNLVLTFVDYRMDDPPKYTVKECKERDATYAAPLRVTARLLNK
                     ETGEIKESEVYMGDFPIMTESGTFVINGAERVIISQLVRSPGIYYEMSRDKTGKKLFT
                     TTVIPNRGAWLEYESDQNDLFYVRIDKNRKIYITTFIRALGLSSNAEIDEFFGYDERI
                     KATLDKDPTQNTEEALLEVYKKLRPGEPPTIDTAKAHIDNLFFNEHRYDLSRVGRYKY
                     NKKLAISDRLKGRVLAENIVDPLTGEILAMKGDKISREKAMEIEQAGVSVAYVDADGV
                     SVKVISNGMVDPLPYMPESITEEELEAIGINENVSFKVLMEVLEKCGDDKNALLEEMQ
                     ARCDDLIPKHITVDDIFASINYLNCLAHGVGHVDDIDHLGNRRVRSVGELLQNQFRIG
                     FTRMERVVRERMTLQAQEMDKITPQALINIRPVVAAIKEFFGSSPLSQFMDQTNPLAE
                     LTHKRRLSALGPGGLSRDRAGFEVRDVHYSHYGRMCPIETPEGPNIGLISYLATFARI
                     NKYGFIEAPFRKVDKKTGVVLDEVVYMTADVEDEYCIAQANEPLDENGRFLNSKVTVR
                     YRDEFREVDASEADFMDVSPKMVVSVATAMIPFLENDDANRALMGSNMQRQAVPLLRT
                     EAPLVGTGMEYKAAIDSGVVVLAKRAGVVTKVSSTRIEIAADDGGTDTYELIKFLRSN
                     YGTCINQKPIVEVGQHVEANEVIADGPATDHGEISLGKNVLVGFMTWEGYNYEDAILI
                     SEKIVREDVFTSIHIEEYQTEARDTKLGPEEITRDIPNVGEDALKDLDENGIIRIGAE
                     VHSGDILVGKVTPKGETELTAEERLLRAIFGEKAREVRDTSLRVPHGEYGVVVDVEVF
                     TRANSDELAPGVNKVVRVYVAQ"
     gene            3709..3996
                     /gene="rpsF"
                     /locus_tag="PLD98_00500"
     CDS             3709..3996
                     /gene="rpsF"
                     /locus_tag="PLD98_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQA04773.1"
                     /translation="MAKNKYESVIVFSAKNGEESIKELVEKFKALIEENGTLESVDEW
                     GLRKLAYPIQFQNEGYYVLYNFESEAAFPAELDRVVKITDGVLRSLIVKKS"
     gene            4009..4440
                     /locus_tag="PLD98_00505"
     CDS             4009..4440
                     /locus_tag="PLD98_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQA04774.1"
                     /translation="MLNNVTLMGRLVADPELRTTPNGISVTSFVIAVDRSYARTNEER
                     KTDFIDLVAWRSTAEFITKYFKKGSMIAVKGSLQTRLYEDKNGNKRKAVEVVVDNAYF
                     CGSKASGNTEYTAPSAPAPSYQSGDADDFTEVLPAEDDLPF"
     gene            4463..4711
                     /gene="rpsR"
                     /locus_tag="PLD98_00510"
     CDS             4463..4711
                     /gene="rpsR"
                     /locus_tag="PLD98_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQA04775.1"
                     /translation="MAYDKADKRAGNRKGRRKVCTFCVEKTEHIDYKDVAKLRRFISE
                     RAKILPRRTTGTCAKHQRELTTAIKRARHLALLPYISD"
     gene            complement(4806..5156)
                     /locus_tag="PLD98_00515"
     CDS             complement(4806..5156)
                     /locus_tag="PLD98_00515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HQA04776.1"
                     /translation="MTVKRGEIYYADLSPVVGSEQGGLRPVVIVQNDIGNKYSPTVIA
                     AAITSQKYKSNLPTHIKVSASNCGLAKDSIVLLEQVRTLDKRRLKEKMGALPEEDMRR
                     INQALSVSFGLTTT"
     gene            complement(5426..6487)
                     /locus_tag="PLD98_00520"
     CDS             complement(5426..6487)
                     /locus_tag="PLD98_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQA04777.1"
                     /translation="MINKLLEVITPQYDAALIISPVNRRYFTRFDSSDGFLIVSEKGS
                     VFFTDGRYIEAAQKTVTVCDCVEAVKPYEQIREYFNKIGAKRIAVEGSKLTVAQYERL
                     KERLPEFEFFADKTLDLGLDGLRRVKTAKELEFIKAAQKTAEDAFQHILTVIKEGVTE
                     RELALELDFYMLKAGAEELSFKTIAVSGANSSMPHGVPSDKKLQKGDMITLDFGAVCE
                     GYHSDMTRTVVLGKANSEQKKVYNTVLKAQNECLSMLRAGVKCSDADKAARSVIEKAG
                     YGDFFSHSTGHGVGLEIHEEPRLSSLVNDVLKSGNVVTVEPGIYIPGKFGVRIEDMAY
                     ITDDGIINLTSSPKELIEL"
     gene            complement(6480..7061)
                     /locus_tag="PLD98_00525"
     CDS             complement(6480..7061)
                     /locus_tag="PLD98_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HQA04778.1"
                     /translation="MSGRAVESARFSHFNAKGKEAMFNLIPKYVFNRITDITPEMVKK
                     MGAKAVAFDLDNTIVYDGGYRLLEGAAEWIESMKKAGIPLFIISNTYRLRSRIISKRL
                     GIMAIGKAGKPRPDSFKKAAEMLGINVSELIMIGDKLDKDIAGANAAGAISVYVKPFM
                     TEKIMYFYHKRLRKREREFMKKHKEKFEVSVFD"
     gene            complement(7062..7238)
                     /gene="rpsU"
                     /locus_tag="PLD98_00530"
     CDS             complement(7062..7238)
                     /gene="rpsU"
                     /locus_tag="PLD98_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQA04779.1"
                     /translation="MSQVKVKENESFDSALRRFKRQTSRDGVIQELRKREHYEKPSVR
                     RKKKSEAARKRRHK"
     gene            7488..7835
                     /locus_tag="PLD98_00535"
     CDS             7488..7835
                     /locus_tag="PLD98_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQA04780.1"
                     /translation="MDREERLNALITELRRGTLTLTVLGALKHEHYGYSLLQFLQSRD
                     INLEANTLYPMLRRLEEQGLLASRWDTSANRPRKYYILSKEGYSVYLELLSEWKRMQR
                     SLYSICEGDLNYE"
     gene            7828..8949
                     /locus_tag="PLD98_00540"
     CDS             7828..8949
                     /locus_tag="PLD98_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04781.1"
                     /translation="MNELIERYIHDVIRRLPEKDRAEIERELRANIYDMLPENYTEED
                     VVRVLKEMGDPASLAEKYRVNPRYLISPASFDSYVYFLKLIIPIVAGVLFVLSVISES
                     VDLLKTGGTDAISLIASSFAHGIEAAVAGAVQAGIWITIGFFIYERVNGKDINIKGEW
                     NPHNLPPIVKQTNKTIPLSDSIAELVLTIVGAIIGIGFFSGLIFAGNFIVIKGTKVNN
                     IFSPEFSDAVIPIIILTAILGIIKHSVRIIFRKWNLAVCLATVLTSLVGMVASVHLLI
                     TYPVFSAELIELLNTLGLNTLKNAPILADRGIYIIIDIIVIIIIVSTVSEVISAFVKT
                     LKPEKYKNKTSPIAFAMGFTAHKKRIYKAFTCKFRNWSG"
     gene            complement(8938..9011)
                     /locus_tag="PLD98_00545"
     tRNA            complement(8938..9011)
                     /locus_tag="PLD98_00545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8977..8979),aa:Gly,seq:tcc)
     gene            complement(9175..10071)
                     /locus_tag="PLD98_00550"
     CDS             complement(9175..10071)
                     /locus_tag="PLD98_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HQA04782.1"
                     /translation="MLLEKLAWRQVQALNKGKKALWDIINGKNLIASLVVLAEVLGCL
                     FFDLPMTPSGPELDLTGYELVFEDEFEGDALDLTKWTHRGEGRQRGGFSADHQVRVEN
                     GNMIIKGEYLENGKFGPGWYAGMVKVKDDYKFGYFEIRCICNPGGGFWSAFWLQSNYS
                     YNPEISRGGVGGAEIDIMEAMSYNSSFLERNGIHQTIWVSGMTGDPTNGTGLNKLAVG
                     KFYGNNIYEEYNTYGLKWDENEYIFYVNGVETARTSFADGTSKVPEQVIVSLCVPQEI
                     NHSKDFTTEFIVDYVRIYQKAD"
     gene            complement(10167..10856)
                     /locus_tag="PLD98_00555"
     CDS             complement(10167..10856)
                     /locus_tag="PLD98_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HQA04783.1"
                     /translation="MDILQVILTSVFSFIALFILTKLMGHKQVSQLDFFDYITGITIG
                     SIAAELATDLEAPWKSTVAMVIYALATIIIGVVTRKIPKAREYLNGASVKLIDNGKLI
                     RENLKKAKLDLNEFLMMCREKGYFDIRNIHTALFESNGKLTILPFSSHRSVTPDDLQI
                     KVPQESLFAEIIMDGRVMEKNLKQLGLDLTWLSKKMDEHNIKSASEVFLAVCDRNKNI
                     AFYKKDSKSGS"
     gene            11005..11682
                     /locus_tag="PLD98_00560"
     CDS             11005..11682
                     /locus_tag="PLD98_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HQA04784.1"
                     /translation="MLIIVIRALVIFVILNILLRIMGKRQIGQLQPIELVITILLSEI
                     SVQPLTQYEASFVTPIITLVILVSLEIIASTLCMKSVTFRNLMQGNSLIIIRNGVLDQ
                     AQIKRLRFTLDDILEALRKKDVFRIEDVQYAIAETDGSLSVLLKPEKRSFTKEDGKIN
                     APDYGIPSVVVSDGKIIQSDFKECGMTYEKLVQKLNSLGKRPEDIMLMTIDKAGNVFV
                     IDKEKNI"
     gene            11679..12059
                     /locus_tag="PLD98_00565"
     CDS             11679..12059
                     /locus_tag="PLD98_00565"
                     /inference="COORDINATES: protein motif:HMM:NF025634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HQA04785.1"
                     /translation="MKRFYISLAMLASVIAFSIYGHVTLYRNFERINEDLKQCVEALE
                     KEDEQGVFEHLSKAEKTWDESKWLIGSVYSHQTKIEMRHLFLLIRELAESGDKENFKE
                     KTEEMIAYIQTSIDSIAVRLYNIF"
     gene            12184..12276
                     /locus_tag="PLD98_00570"
     tRNA            12184..12276
                     /locus_tag="PLD98_00570"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12220..12222,aa:Ser,seq:gga)
     gene            12312..12404
                     /locus_tag="PLD98_00575"
     tRNA            12312..12404
                     /locus_tag="PLD98_00575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12346..12348,aa:Ser,seq:gct)
     gene            complement(12742..13530)
                     /locus_tag="PLD98_00580"
     CDS             complement(12742..13530)
                     /locus_tag="PLD98_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HQA04786.1"
                     /translation="MSDTMILVAGLLLPLIGTILGAAMVFFLKNEMKPKVQKALLGFA
                     SGVMIAASVWSLLIPAIEMTEEQGGVAWIPCAVGFLIGIGFLLLMDTLIPHLHINTDK
                     AEGHKSKLSKALMLILAVTLHNIPEGMAVGVVLAGFSSGNANISAAAALALSIGIAIQ
                     NFPEGAIISMPLVATGISKRKAFSYGFLSGVVEPIGAILTIFLTSLVTSVLPYILAFA
                     AGAMIYVVVEELIPEAQDGEHSNVGTIGVALGFTLMMILDVALG"
     gene            13812..14771
                     /locus_tag="PLD98_00585"
     CDS             13812..14771
                     /locus_tag="PLD98_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQA04787.1"
                     /translation="MKKAKKLISVLLILSMLLPLCFTANAATVTQLPFPDSAFFEIGD
                     YSIHYRFRPSAASEHKGRFVFLHGFLGSTVSWKPLSDRLIQEGYDCLLVDLPSFGYST
                     RETAQVHPIPREEIVASLMQELAPAEKWYVAGHSMGTTVAMNIACRYPELVEALLLYA
                     ATSDTKTKFTNLPDPVLQVYGGVYNVIFKMIITLASNPVFGKIIMRNALYDANRDFET
                     RQKYLEPLKLENTGMGILFMRKRSTPLDFEQLKQLEMPVMLCVASDDEFVPNDSENAI
                     RLRNSLPAHTVYYVMPDSTHSLIESKADETFAVTFDFINGILH"
     gene            14933..15247
                     /locus_tag="PLD98_00590"
     CDS             14933..15247
                     /locus_tag="PLD98_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04788.1"
                     /translation="MAKQARMIIHKEYKKGDIDPKIYGSFIEHLGRAVYSGIYEPGHP
                     TADENSFRQDVLELVKKLKVPAVRYPGGNFVSGYRWEDGTGDKKNRPVCADLAWHTIE
                     TN"
     gene            15302..16447
                     /locus_tag="PLD98_00595"
     CDS             15302..16447
                     /locus_tag="PLD98_00595"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HQA04789.1"
                     /translation="MAVNLGTRGPEDARNLVEYCNGTMPTQYAEMRRKNGFEEPFAIK
                     YWCLGNEMDGHWQICHKTAAEYGRIALEAGKLMKMIDPDIKLVLCGSSNYNMSTFGDW
                     EWTVLNEAYSVVDYISLHQYYSRSEFKSTEDFLGRASHMDSFIKGVAAICDAVQAKKH
                     SKKKIHLSFDEWNVWDQRVWGGDPEPGEPWQQKPHKLEQIYNFEDALLVGTMLSTLQN
                     NCDRVKIACMAQLVNVIAPIMTEEGGKAWVQTIFYPYYYASVFGRGECLDAKVYSDSY
                     RTSDGYNVPYVNASVVLSEKKDKIYVFASNLSLEEDMELTLSFEAFGEITPISKTELY
                     CDDLKAVNTKDFEAVSPTTVQLNGKSDTSHTLSLKKHSWNMFEFKIQ"
     gene            complement(16570..17142)
                     /locus_tag="PLD98_00600"
     CDS             complement(16570..17142)
                     /locus_tag="PLD98_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQA04790.1"
                     /translation="MSKKTNTVLLIVFFALIIAMSAFLYNELTREYAPDQDLIQIGAE
                     TKEGKGNLKKAEDFMVVNIDGKYVKLSDMLGRPVVVNFWASWCSPCKEEMPEFNKVYE
                     EYGTAVAFMMVDLVDGNRETREKGLEFITSRGYRFPVYFDMYQEAAKAYGITSIPMTL
                     FIDKDGYIVTKAIGAIDEDTLKKGIELIRK"
     gene            complement(17155..17820)
                     /locus_tag="PLD98_00605"
     CDS             complement(17155..17820)
                     /locus_tag="PLD98_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HQA04791.1"
                     /translation="MQYLLLFLEGIITFISPCILPLLPVYVSYFAAGQAAGKKALINS
                     IGFVSGFSAMFIAMGAFAGTVGRLLQSYSTVVNIVTGLIVIVLGLNFLGVLKIGLLNR
                     SGGKDREIKELGFFSSLLFGIIFSIGWTPCVGAFLGSALMMAAVSGSAFKGITMLLAF
                     SLGLGLPFIMSAILLDRLKTAFDFIKKNYKVINTLSGVLLIIVGILMATGIMGRFLSL
                     LTF"
     gene            complement(17877..18158)
                     /locus_tag="PLD98_00610"
     CDS             complement(17877..18158)
                     /locus_tag="PLD98_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQA04792.1"
                     /translation="MEKKAKQIAELLKVLANDNRLMILCALMEGPKTVSRISEKVPNI
                     TQSALSQHLAILKAHSILDFEKSGQNITYWIADHRVEEIINVLKKHYCN"
     gene            complement(18103..18735)
                     /locus_tag="PLD98_00615"
     CDS             complement(18103..18735)
                     /locus_tag="PLD98_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQA04793.1"
                     /translation="MRTKRWYDYLWIFSLVYLFLGFFNILFAYIGLLCFTVPLMIAII
                     RGDKSYCNKYCGRGQLFTILGDKLGISFKKDPPKFLRSSIFRYGFLVFFLSMFFMMIY
                     STYLVFAGAPLKQAVTLLWLFKLPWNWSDVSFVPAVVAQFAFGFYSVMLTSTVLGLVT
                     MIIFKPRTWCVYCPMGTMTQGICKIKNKGESVSGEKSKTNCRAFEGACQR"
     gene            complement(18772..18960)
                     /locus_tag="PLD98_00620"
                     /pseudo
     CDS             complement(18772..18960)
                     /locus_tag="PLD98_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            19104..19532
                     /locus_tag="PLD98_00625"
     CDS             19104..19532
                     /locus_tag="PLD98_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04794.1"
                     /translation="MNGNAELLNFIYQNSQMGVNTLSQLMDIAEDEEFRKHLQQQYEE
                     YKDIHEKAKQALNENGYDEKGISALDKIKTYIMVNIQTLTDKSSSHIAEMLMVGSNMG
                     IINAIKNLKKYSDAEQSIKSLMERLLKLEENNVQQLKNFL"
     gene            complement(19559..20020)
                     /locus_tag="PLD98_00630"
     CDS             complement(19559..20020)
                     /locus_tag="PLD98_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HQA04795.1"
                     /translation="MVEYAAKWALSRNPRYANFDGMGGDCTNFVSQCIYAGCRVMNYT
                     PIYGWYYINLNNRSPAWSSVQYLYKFLINNKEVGPYCVETDREHIQKGDIIQLRDSSG
                     TYYHSLVVMSNNNGNILISAHTYDALNRPLDSYVGADGMRFLHIVGARKWV"
     gene            complement(20162..21223)
                     /gene="ftsZ"
                     /locus_tag="PLD98_00635"
     CDS             complement(20162..21223)
                     /gene="ftsZ"
                     /locus_tag="PLD98_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQA04796.1"
                     /translation="MAFEISNEFEEVTKIKVIGVGGGGGNAINRMINANVQGVEFIAI
                     NTDKQVLSNSKATHKIQIGEKTTSGQGAGARPEVGKKAAEESSDLIEEALAGTDMVFI
                     TAGMGGGTGTGAAPVVARMAKDKGILTVGIVTKPFEFEGRRRMQQAEAGISELAQHVD
                     SLIVIPNERLKLVTDQKLTFLNAFEIADDVLRQGVQSISELIKIPGLINLDFADVTSV
                     MKDAGHAHMGVGKASGKDKAVQAASAAISSPLLETTIQGAKGVIINITASPDITLDDV
                     DAAATTISNAADKDANIIFGVAINPDLDDEVIVTVIATGFGKDGNAIKAAGSEEAEGD
                     AFGFDDDLTDIFDMFKNRN"
     gene            complement(21420..22328)
                     /locus_tag="PLD98_00640"
     CDS             complement(21420..22328)
                     /locus_tag="PLD98_00640"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HQA04797.1"
                     /translation="MKGQRKNSVPDRQLSNTERVIRDNEKRRKKKRRRDRMIYIFLAL
                     FVAGAGIVLSLTVFFHIEVITATTDSLYYSEAEIIEASGIKLGENIFLQSLKNVSAKI
                     EKDLPYIESAKTKISLPNKIIIQTTDAVPALAVEAGDTYYLLSKNAKILGTTGMAIPP
                     DPTALVRGLTLKEPELGSIAEFNEDGGYELLIQLLTSIETNGLEKITEIDLTDTVDLR
                     VWIEDIIRLDLGSINDVDKKLSFAKSVIAAEKEKNIVNPIYIDLRILREAHVRDITTS
                     AETQPEPSKEPESESESTSEKQTERV"
     gene            complement(22378..23634)
                     /gene="murA"
                     /locus_tag="PLD98_00645"
     CDS             complement(22378..23634)
                     /gene="murA"
                     /locus_tag="PLD98_00645"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQA04798.1"
                     /translation="MGKYIIEGQRKLRGEIDVQGSKNSALPILAATVLCKGVSVIHNC
                     PRLLDIAATINILEHLGCKVKREGSTVIVDSTDITRYDIPENLMHEMRSSIIFLGAVL
                     GRLGKANISTPGGCEIGLRPINLHLDAMEKFGVELEEGHGRLEFTAKNGLKAATITLS
                     FPSVGATENIILAASTAVGTTVIINAAREPEISDLADFLNGCGARISGAGESTVVIEG
                     VKSLSGTEHTVIPDRIAAATYMGAAAITQGSVTLRNIIPSHLGSVISVFEEMGCDISI
                     NGRNLTLVAPSRLQRVKTIRTMPYPGFPTDLQAPVMALTSVAKGTSVIIETIFENRFK
                     HVGQLARLGAKIMVDGRTAVVEGVDRLTGAEVVSPDLRGGCALVLAGLSAKGTTVVDA
                     INHIDRGYDGLEDVLSSMGANIKRVE"
     gene            complement(23821..24942)
                     /gene="murG"
                     /locus_tag="PLD98_00650"
     CDS             complement(23821..24942)
                     /gene="murG"
                     /locus_tag="PLD98_00650"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQA04799.1"
                     /translation="MRVVFAAGGTGGHINPALAAAGEIRRRFPDADILFIGTKDKMEA
                     KLVPGAGFDFKTIEISGFQRKLTPQNIKRNIITLKRLITSGSQVKKILKEFKPDAVIG
                     FGGYVSGPVVRTAAKMGIKTAIHEQNAFPGITNKTLAKYADTVMTAVPEATERLKCKN
                     PPVVTGLPVRGAILCANKDFSRAELHIKPNEILIFSTGGSLGARTINELMLEVITELH
                     KEKNLRFIHGHGQYGHWFPEKLKERGIDIDNSPNIDVREYIYNMDVCLSAADIVVSRA
                     GASAIAEIQALGKAAILIPSPNVSENHQYYNAKALADKNAAVLFEEKDLDAKKFVQFI
                     KELVQDKERRDELGANAKKMAVTDATERICDEILKLVEK"
     gene            complement(24967..26223)
                     /locus_tag="PLD98_00655"
     CDS             complement(24967..26223)
                     /locus_tag="PLD98_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HQA04800.1"
                     /translation="MAEKAVKRRRKSLIRMAVAEGSFDLVFFITVTLLLVIGLVMLFS
                     ASYPYALQKYKNSTHFFYRQLIFAVMGFVIMIVLSRVRFEYFRLAALIGFFMSLFLLV
                     LVLFTPAVKEGHHRWLTIPGLSGFTIQPSEIAKLGLIMFCAWQFDKNYKKINSKELIK
                     APFAHSLNRITNGRIRITESFASLVLYGGIILVFAVLVFLENHLSGTILMLCIGLIML
                     YLGGFDRRWFIAGFLLVAFVVAVFVMFPSLRPNILQEYMTERIVAWLDKDFEPTGARW
                     QVNQALYAIGSGGFFGAGLGNSKQKYMYVSEPQNDMIFSIVCEELGFVGAVIILFLFA
                     VLVWRGVVIGLKSKSRFGALLSMGIVFQVGLQTVLNIAVATDSIPNTGISLPFFSYGG
                     SSLLILLAEMGMILSVSRYTSLKKNN"
     gene            complement(26272..27300)
                     /gene="mraY"
                     /locus_tag="PLD98_00660"
     CDS             complement(26272..27300)
                     /gene="mraY"
                     /locus_tag="PLD98_00660"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQA04801.1"
                     /translation="MNKWFALILSALVGFGTAYALGYVVIPWLHKLKFGQTILDIGPS
                     WHKKKQGTPTMGGILFIAGVIASLIVTVLTYRFMGDDIISGDAVVPNTVKVKIFGGLL
                     MAIAFALVGFADDYIKVKRGRNLGLTIMQKSIAQVIIMGLYLYTLSSVGATYIYIPFV
                     GVRDIGIFYWILGVLAIYCTVNAVNFTDGIDGLCASVTAVACVSFAVIAVLRGMFGVS
                     LISSALFGSLIGYLIWNWHPAKVMMGDVGSLFLGGMVIAISYALDAPWLLLTVGIIYV
                     IEFLSDVIQIVYFRLTGGKRIFKMAPIHHHFEKSGWSEVKIVKVFSLIGALGGLFSIL
                     ITWFGRIK"
     gene            complement(27359..28714)
                     /gene="murF"
                     /locus_tag="PLD98_00665"
     CDS             complement(27359..28714)
                     /gene="murF"
                     /locus_tag="PLD98_00665"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQA04802.1"
                     /translation="MIKLGTKEIAKAVGGKAEKDITIDGVFTDSRKPVKGGLFVAIEG
                     ENFDGHDFIGAAVKEGAAAVLCRKAPDADVPYILVDDTKAAYQKLAAYYRQKTGVKVV
                     GITGSVGKTTTKDFTYLCFSAKYNTFRTQGNLNNDIGVPATLLSIDACHQAAVVEMGM
                     NHAGEISLLSKIAASDAAIITNVGVTHIENLGSRENILKAKLEILDGLKEGSPLIINA
                     DNDMLSGLDIKGFRLIRCSMEKKADVYANNIIETAEGSYFQVNVLGEKGSVKLPAAGR
                     HNIINALLALAAGKVFDIPVEDMCKALQLYEPSGMRQRVKVVKGITFIEDCYNASPDS
                     VNASLSVLKNMTVKRRIAVLGDMLELGDIAVSAHESIGKSAKDNNVDILFTYGELSKN
                     TARTAIDSGVSEVYTFDSHDALAEKLYSILRSNDGVLVKGSRGMKLENVLNKLYDLLG
                     E"
     gene            complement(28735..30171)
                     /locus_tag="PLD98_00670"
     CDS             complement(28735..30171)
                     /locus_tag="PLD98_00670"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQA04803.1"
                     /translation="MKLSELLKKVNVLSDYKDTEITAVTDNSNKITEGCAFVCIKGNK
                     FDAHNVAAEALQKGAAAVIVERDLGLDRQVICDNTRKAYSLMCSAFFSNPSEDLKLIG
                     ITGTNGKSTTAILIKSIMDKLGIKAGLIGTVKNMIGDTELEAVLTTPEPFELQSLFRR
                     MADENCEYCVMEVSSQALAQERVEGCHFRAAVFTNLTQDHLDYHGSFEAYADAKQKLF
                     RMCDISVLNMDDSYYKHMAEGSNSKVVTYSIEDDAADFTAKNIQYRQTAVSYELVTKG
                     DIGRAHVGIPGKFSVYNSMAAAVTLVELGFGMKEVLEALEKAEGVKGRVEIVPTNTDF
                     TVIIDYAHTPDGLVNVITALRQIAAGRIITLFGCGGDRDKTKRPIMGDVASQLSDVVV
                     VTSDNPRTEDPQKIIDDILEGIKNTKAKVFVEPDRRKATAIALKEAKAGDIVLLAGKG
                     QETYQILKDKTIHYDEREVVKELLEEMK"
     gene            complement(30192..32411)
                     /locus_tag="PLD98_00675"
     CDS             complement(30192..32411)
                     /locus_tag="PLD98_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQA04804.1"
                     /translation="MKTQKLLATRGLIILALLVVAGFLSLSGRLAYLQLVKGEEYKAK
                     AEAQQLSDTVIKAQRGTIYDRNMKVLAQSASVWLVYINPSKIKTEATKQLVIDGLSQI
                     LEVNPETIKKKAERTHVGYELIKGQIEKPVMEEVLKFIQQNKLTGIVNIDPDTKRYYP
                     YSGFAATVLGFTGKDDIGRAGLEYLYNDILTGTAGRVVTAKDGRLEAMPNQYESTYDA
                     IPGTNLVLTIDEVVQYYLESALNHSLQETKAKNVYGIVMDVKTGAILAMANLPDFDPN
                     NPDLILDENVRKQIESIQDEKERNEALLNAKYAQWRNRTISDSYEPGSVFKVVTVSAA
                     VEEGLVNNNFGYTCTGSIAVADRIIRCWKHGGHGSQRLPDLLKNSCNPFAVTIAKLVG
                     TEKYYEYFEAFGLTEKTGIDLPGEAAPNEGVTYHSKKNFGISQLSSYSFGQSFQVTPI
                     QMITAISAVANGGKLMQPYVVAKEIDAEGKVIKEISPIVKRQVISEKTAAIIREHLEA
                     VVTSGTGKNAYVAGYHVAGKTGTSQKLGKDGAYVASFVGFAPANDPKVAVLVAIDEPV
                     GEHGGGAVAAPVAGEIFDQVLPYLNIEPQYSDNELALLVSYAPNVVSKSVNEAKNEIT
                     RAGFTARVIGSGDTVVAQSPESDRAIPKKGVVVLYTESKNTSQEAVVPDFKGMTVSAA
                     NKAAINNGLNVKIAGSSLNSGEVFAYRQSIAPGEKVPLGTVVTVYFKSNVDVSDSDE"
     gene            complement(32525..33073)
                     /locus_tag="PLD98_00680"
     CDS             complement(32525..33073)
                     /locus_tag="PLD98_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04805.1"
                     /translation="MPSYYGTEAYDFSLFEPYEKKVTSSAAPKRAPQKKTKEHPHLKV
                     YPKVQKKTDTKKAIKLQMRANMQKSVKVFAVMALLFSMLSVKIYCNVVLDETTKEITK
                     LEQKLAVEKSEAVRLQMAIDSIVSVKNVDEYAENVLGMMKMEPGRESYINLSKGDEVV
                     VSGGKEVNEEPSIFRQLLAYIF"
     gene            complement(33100..34032)
                     /gene="rsmH"
                     /locus_tag="PLD98_00685"
     CDS             complement(33100..34032)
                     /gene="rsmH"
                     /locus_tag="PLD98_00685"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQA04806.1"
                     /translation="MEFVHKSVLLAESVDALEIKENGIYVDATAGGGGHSAEILRRLK
                     NGRLIAIDRDPDAVSVLTERFKGDDRVTIVNKNFSDIKQVLSELNIDGVDGILADLGV
                     SSYQLDNDERGFSFHKDAPLDMRMDKSGLSARDVVNGYSESQLRDIIFRYGEEKFAKS
                     IAANIVKERAKKPIETTSELAEIIKYSIPARARREGGHPARRTFQALRIEVNRELELL
                     PEAIDNMFSVLNGNGILAVITFHSLEDRIVKQIFASYCKGCECPKDFPVCVCGKVPQG
                     ELPFKSIEPSEAEIAENNRSRSARLRAVRKLPKE"
     gene            complement(34051..34476)
                     /gene="mraZ"
                     /locus_tag="PLD98_00690"
     CDS             complement(34051..34476)
                     /gene="mraZ"
                     /locus_tag="PLD98_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQA04807.1"
                     /translation="MLFQGDFKHTIDSKNRIFIPARFREALGDSFIVYPAPDGCLFIF
                     TNEKWESIANQIKGQSATIEDRKRQRRAFYGVLTVEPDKQGRITLSQFLVDHAGLKKD
                     VMIFGMTDRIEIWDLDRWNNEAMASSEEMTDDIYPEIIY"
     gene            34882..35004
                     /locus_tag="PLD98_00695"
     CDS             34882..35004
                     /locus_tag="PLD98_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04808.1"
                     /translation="MDQLMAFLEKLIQFLIDILYSLGIELDYDKLFPTEPTSEE"
     gene            35219..35295
                     /locus_tag="PLD98_00700"
     tRNA            35219..35295
                     /locus_tag="PLD98_00700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35253..35255,aa:Pro,seq:cgg)
BASE COUNT         9169 a         8193 c         7814 g        10129 t
ORIGIN      
        1 ttctgtgcga cataaacacg aactacctta tttacgccgg gcgcgagctc gtcgctgttt
       61 gcccttgtaa agacttcgac atccacaaca acgccgtatt cgccgtgggg cactctgagg
      121 gaggtgtctc tgacttctct tgccttttcg ccgaaaatcg ctctaaggag tctttcctcg
      181 gcggtaagct cggtttctcc tttgggagtt accttgccga caagaatgtc gccagagtga
      241 acctccgcgc ctatgcggat aatgccgttt tcgtcaaggt ctttaagggc gtcttcacca
      301 acattgggaa tatcccttgt gatttcctcg ggaccgagct ttgtgtctcg tgcctcggtc
      361 tgatattcct caatatgaat ggaggtgaat acatcttctc ttactatttt ttcactgatg
      421 aggatagcgt cctcgtagtt gtagccttcc catgtcatga agccgacaag gacattcttg
      481 ccgagggaaa tctcgccgtg gtcggttgcg ggaccgtctg cgatgacttc gtttgcttca
      541 acatgctgac cgacctcgac aatcggcttc tggttgatac aggtgccgta gttggagcgc
      601 aggaacttga taagctcata ggtatcggtg ccgccgtcgt cggctgcaat ctcgattctg
      661 gttgaagata cctttgtaac aacacccgcg cgctttgcga gcacgacaac gccggagtcg
      721 atagctgcct tatactccat gcctgtgccg acaagcggag cctctgttct gagaagcggt
      781 acagcctgcc tctgcatgtt ggagcccatg agagcacggt ttgcgtcgtc gttctcaagg
      841 aagggaatca ttgctgtagc aacggaaact accatcttgg gcgagacatc catgaaatca
      901 gcctcggagg cgtcaacttc tctgaactca tccctgtagc gtaccgttac cttgctgttt
      961 aagaatcttc cgttttcatc aagaggctcg tttgcctggg caatgcagta ctcatcctcg
     1021 acatcggcgg tcatatagac tacctcgtca agaactacgc ctgtcttttt gtcaaccttt
     1081 ctgaagggag cctctataaa gccgtattta ttgattcttg cgaaggttgc aagataggaa
     1141 ataagaccga tgttcggacc ttcaggagtt tcgataggac acatacggcc gtagtggctg
     1201 tagtgaacat ctcgaacctc gaatcccgct ctgtctcttg aaagaccgcc ggggccgagg
     1261 gctgaaagcc ttctcttgtg cgtgagctcc gcaagcgggt tggtctggtc catgaactgt
     1321 gaaaggggcg atgaaccaaa gaactccttt attgcggcaa ctacgggtct tatattgata
     1381 agagcctgag gagtaatctt atccatctcc tgtgcctgta aggtcattct ctcccttacg
     1441 acacgctcca ttcttgtaaa gccgattctg aactgattct gcaaaagctc acctacgctg
     1501 cggacgcggc ggttgccgag gtggtcgata tcgtccacat ggcctacgcc gtgggcaagg
     1561 cagttgagat agttaatgga cgcgaatata tcgtcaactg taatgtgctt ggggataagg
     1621 tcgtcgcagc gggcctgcat ttcctcaagc agggcgttct tgtcgtcgcc gcacttttca
     1681 aggacttcca taagaacctt gaaggaaaca ttctcgttaa tgcctatggc ttcaagctct
     1741 tcttcggtga tgctttcggg catataaggc agcgggtcta ccatgccgtt tgaaataacc
     1801 tttaccgaaa cgccgtcagc gtcgacataa gcgacggaaa cgccggcctg ctcaatctcc
     1861 atggcctttt cgcggctgat tttgtcgcct ttcatggcaa gaatttcgcc ggttaatggg
     1921 tcaacaatat tctcagccaa aacacggcct ttgagcctgt cggagatggc aagctttttg
     1981 ttgtacttat atctgcccac tcttgaaagg tcatatctgt gctcattaaa gaacaggtta
     2041 tcgatatgag ccttagcggt atctattgtg ggaggctcgc cgggcctgag ctttttgtaa
     2101 acctcaagca gcgcctcttc ggtattctga gtcgggtctt tgtcgagtgt ggctttgatt
     2161 ctctcgtcat agccgaagaa ctcgtcaatt tccgcattgg atgaaagacc caaagctctg
     2221 ataaatgttg tgatatagat ttttctgttc ttgtctatgc gcacataaaa gaggtcgttc
     2281 tggtcggact cgtactcgag ccatgcgcct ctgttgggga taacggtcgt ggtaaacagc
     2341 tttttgcctg ttttgtcacg gctcatttca taatatatac cgggtgaacg gacaagctgg
     2401 gaaattatta cgcgctctgc gccgttgata acgaatgttc cgctctcggt cattatagga
     2461 aagtcgccca tatagacttc gctttcctta atctctcccg tttccttatt taaaaggcgg
     2521 gcggtaacgc gcagaggtgc tgcgtatgtg gcgtctcttt ctttacactc cttgacggtg
     2581 tactttggcg gatcgtccat acgataatct acaaaggtca gtacaaggtt gccagaatag
     2641 tcggttattt cgccgatgtc gcgaaatacc tcgctcaatc ccacatcgag aaaccacttg
     2701 tatgaattct tctgtacttc aataaggtta ggcatctcca taacctcatt gatgcgtccg
     2761 aagctcatac gggttctctt gcctctttta acaggcttaa cattcaccaa ggacaatcac
     2821 tccgttcttt ctccgttatg gactctctta gctgcatcag tatgaaaatc aaagaagtat
     2881 aaagctgaag gtgcctttag caaaacttgt ccgctttagg aatgtattta aaaaggaaaa
     2941 atatttttaa atacattctt taaataaagg cttgattttc atatcaaata cggttataat
     3001 tgagctagca gcatgcaatg gagcatggca ctccattatg atgcagtata acatattaca
     3061 ccagtaggtt atatttgtca agcatatttt acaatataat gtaatatttt attcaataat
     3121 ctaaaagcat tgatattcta agcgtcaatg ctttttttaa tttttaaaac ttcatcaaaa
     3181 agaccgacat tttgagggta agcaaagagt tggtaactaa ttggaaacaa aaaaatcccc
     3241 cactttgtaa aaataagtgg ggttttttaa gtttaaattg aaaaatttaa gctgcctatc
     3301 agatttcggc caatcaattg cttagcgcgc ccaactggac agagcggtct tttcatccat
     3361 catggccttc ccgccttgca actaatatgg ttttctctct gaacttctgg gattaagtat
     3421 agtatacttg gagttttttg aaagaacact tcaaactctt aatttaacgc cttctgttct
     3481 ccactcttca aaaggaatcg caagttttat ttgcctcatt gatttttagg tcattaatgt
     3541 ttataatatc gctaaggaag aataatagat aaaaattcct tgacaatatt gacaggtgaa
     3601 tatataattt aatcctgtca aagtatgacg cacgcaatct gtgttttttg attgcgttat
     3661 ccttgctccg agtaaaatcg gggccgaagt ccaaaaggag gtgcgaatat ggcaaagaac
     3721 aagtacgagt ccgtcatcgt gttttccgca aagaacggtg aagagtccat aaaggagctt
     3781 gttgaaaagt tcaaggctct tatcgaggaa aacggtacac tcgaaagtgt tgatgagtgg
     3841 ggtcttcgta agctggctta cccgattcag tttcagaacg aaggttacta tgtactctac
     3901 aactttgagt ccgaggctgc gttccccgca gagcttgacc gcgttgtcaa gattactgac
     3961 ggcgttctgc gttcactgat tgtaaaaaaa tcataaggat gaagcaatat gttgaataat
     4021 gttactctga tgggcagact tgtcgcagat cccgagctta gaacaactcc taacggcatt
     4081 tccgttactt cgttcgtaat tgcggttgac agatcctatg cgagaacgaa tgaagagcgt
     4141 aagaccgatt ttatcgactt ggtagcatgg cgttcaacag ccgagtttat taccaagtac
     4201 tttaaaaaag gttcgatgat agccgttaag ggttcgcttc agacccgctt gtacgaagac
     4261 aaaaacggca ataaacgcaa ggctgttgag gttgttgtgg acaacgccta tttctgcggc
     4321 tccaaggcct ccggcaatac ggaatacact gcgccttcgg cacctgcccc gtcatatcag
     4381 agtggcgacg cagacgattt tactgaggtc cttcccgccg aggacgattt accattctga
     4441 ttctaaatta aggaggatag ctatggctta cgataaggct gacaagcgcg caggcaacag
     4501 aaaaggcaga agaaaggtct gcactttctg cgttgagaag actgagcata tagattataa
     4561 agatgttgca aagctccgca ggttcatctc cgagagagca aagatactcc ccagaagaac
     4621 aaccggcaca tgcgcaaagc atcagagaga gcttacaaca gctattaagc gcgcaagaca
     4681 tcttgcatta ctgccctata tcagcgacta atgaaattat ggcgaacggc aaaaccgttc
     4741 gcctttttta tttgctgcta aaaaagaaag ccctgacgcg tggtcagggc tgtttgattt
     4801 ttttgttaag tagttgtgag tccgaaactt accgataatg cctggttgat gcggcgcata
     4861 tcttcttcag gcagcgcgcc cattttttcc ttgagtcttc tcttgtcaag tgttctgacc
     4921 tgttctaaga gcactattga gtcttttgcg agtccgcagt tgcttgcgct aacttttata
     4981 tgtgtcggaa gattgctctt atatttctga cttgtaatgg cggctgctat taccgtgggg
     5041 ctgtatttgt tgcctatgtc gttctgaact attacgacag gcctgagacc gccttgttct
     5101 gaaccaacga caggacttaa atcagcgtag tagatctctc ctcgctttac agtcatttta
     5161 tcactccccg attaattttc ctgtcaaaag tatttttgcc agatgtcatt aaaaataaac
     5221 atcccgtctt aaatttgtgg tgagtaatcc aagactgagt caagccaata acagtatatt
     5281 agccttttag aaaattcgta ctaccctcac cctccttgaa tttaaattta gattgcatta
     5341 tagtgtcggt atttttttct gtttttattc atcggagagc aaaacaaaaa agcccgcttt
     5401 aaagcggact tataagttat atttgttata attcgattaa ttctttgggg gatgaggtta
     5461 agttaataat gccgtcatct gtgatatatg ccatgtcctc gattctgacg ccgaactttc
     5521 cgggaatata aatgcccggc tctacggtta ccacatttcc cgatttaaga acatcgttca
     5581 caagggagga aagccttggc tcctcatgga tttcgagccc taccccgtga cctgtgctgt
     5641 ggctgaaaaa gtcgccgtag cccgcctttt ctataacgct tctggccgcc ttgtcggcat
     5701 cggagcattt aactcccgct cttagcattg aaaggcattc gttctgagcc ttgagaacgg
     5761 tattgtaaac cttcttttgc tcactgtttg ccttgcccag aactacggtg cgggtcatgt
     5821 cgctgtggta gccctcgcaa accgctccga aatcaagggt tatcatatct cccttttgga
     5881 gttttttgtc tgaaggtact ccgtgaggca ttgaggaatt ggcgccgctg accgctatgg
     5941 tcttaaagga cagctcctca gcgcccgctt tgagcatata aaaatcaagc tcaagagcaa
     6001 gctccctttc ggtaaccccc tctttgatta cggtgagaat gtgctgaaaa gcgtcttcgg
     6061 ctgttttttg agcggcttta atgaattcca gttccttggc ggttttgact cgtcttagcc
     6121 cgtcaagacc caaatccaag gttttgtcgg cgaaaaactc aaactcgggc agtctctctt
     6181 tcagccgttc atactgagcc accgtcagct ttgagccttc aacggcaatg cgctttgcgc
     6241 ctattttatt aaagtattcc cttatctgct catatggctt taccgcctct acgcagtcgc
     6301 agaccgtaac ggttttttgg gctgcctcga tgtatctgcc gtcggtgaaa aatacgcttc
     6361 ccttttcgga aacgataaga aaaccgtcgg atgaatcaaa gcgggtaaag tacctgcggt
     6421 ttaccggaga aataattaag gcggcgtcat actgcggggt aataacctcc aaaagcttat
     6481 taatcaaaga cgctcacctc aaacttttct ttatgctttt tcataaactc gcgttctctt
     6541 tttctgagcc gcttatgata aaaatacata atcttctctg tcataaaggg ctttacatag
     6601 acggaaatgg ctcccgcggc gttcgctccc gcaatatcct tatccagctt gtcgcctatc
     6661 atgataagct ccgaaacatt aatgccgagc atttcggctg cctttttaaa actgtcgggc
     6721 ctcggcttac ctgctttgcc tattgccatg atgccgagcc ttttggaaat aatccttgag
     6781 cggagcctat atgtattgga tatgataaaa agcggtatcc ccgctttctt catactctca
     6841 atccactccg ccgcgccctc aagcaagcga tagccgccgt catatacaat tgtattgtca
     6901 aggtcaaagg cgacagcctt tgcccccatt ttttttacca tttccggcgt tatgtcggtt
     6961 attctgttaa aaacatattt ggggataaga ttgaacatag cctctttccc ttttgcgtta
     7021 aaatgagaaa aacgagcgga ctcaacagcc cgcccactca attacttatg cctgcgctta
     7081 cgagctgcct ccgacttctt cttgcgtctt accgaaggtt tttcgtaatg ctctctctta
     7141 cgaagctctt gtataacgcc gtcacgggag gtctggcgtt taaagcgtct gagtgcgctg
     7201 tcaaacgact cattctcttt aacttttact tgactcatct atattccctc cttttcaacg
     7261 aggagtaatc cataatgttt acaataccgt tacattatac aacagcgttt tttgactgtc
     7321 aacacgatta tatgacaata tttaaaattt aatggggcta atattttgta cgcttttacc
     7381 tttatattat attatcacat tcagattata ctatacccaa gcaaaaaaaa tattttataa
     7441 ttgacaatat tgtgcgtcac acagtataat gttactgagg tgatccagtg gacagagagg
     7501 aacgattgaa cgccctgatt actgaactta gaagaggcac gcttactctt acggttttgg
     7561 gcgctcttaa acatgagcat tacggctact ctcttttgca atttttgcag agcagagata
     7621 taaatttgga agcgaatacc ctttatccaa tgctgagaag gcttgaggaa caaggactcc
     7681 tcgccagcag atgggacaca agcgcgaaca gaccgagaaa gtattatatt ctcagcaaag
     7741 agggctattc tgtttatttg gagctgctca gtgaatggaa gaggatgcaa aggagccttt
     7801 attctatttg cgaaggggac ttgaactatg aatgaattga ttgagagata tatacacgat
     7861 gtaatacggc gtttgcccga aaaggacaga gcggagattg aaagggagct gagggcgaac
     7921 atctacgata tgctgcccga aaactacact gaagaagatg tggtcagagt gctcaaagag
     7981 atgggcgacc ccgcgtccct tgcggaaaaa tacagggtaa atccccgata tctcatctcc
     8041 cccgccagct ttgacagcta tgtttatttt ttaaaactga ttattccgat tgtcgccggt
     8101 gtcctttttg tgctcagcgt tatttcggaa tcggttgacc tgcttaagac aggcggcaca
     8161 gacgcgataa gtcttatcgc aagttccttt gcccacggaa tcgaggcggc tgttgcgggc
     8221 gccgttcagg caggcatttg gattacaatc ggtttcttca tttacgagag ggttaacgga
     8281 aaggatatta acattaaagg tgaatggaat ccgcacaacc tccccccgat tgtaaagcag
     8341 accaacaaga ccattcccct atccgacagc attgcggagc ttgtgctgac tatcgtcggc
     8401 gcgattatcg gcataggatt tttcagcggg ctgattttcg cggggaactt tattgtcatt
     8461 aagggaacta aggtaaacaa catattctcg cccgagtttt cggacgcggt gatacccata
     8521 atcatactta cggcgattct cggaattatt aagcacagcg tgagaatcat attcaggaaa
     8581 tggaaccttg cggtttgctt ggcaacggtg cttacctcgc ttgttggcat ggtcgcgtcg
     8641 gttcatctgc ttattaccta ccctgttttc agcgctgaat taattgagct tttgaacacg
     8701 ctgggactta atacattgaa aaacgctccg attttggcgg acagaggaat ttatataatc
     8761 attgacatta ttgtgataat cattattgtt tctaccgtat cggaagtaat atcggcattt
     8821 gtaaagacat tgaagcccga aaagtataaa aataaaacaa gccccatagc ctttgctatg
     8881 gggtttactg cgcataaaaa aagaatttac aaggcattta cctgtaaatt ccgtaactgg
     8941 agcgggtgat gggaatcgaa cccacacgac cagcttggaa ggctggggtt ctaccattga
     9001 actacacccg cattcgcgac attcggaatt atagcacaag tcaattttac agtcaagggg
     9061 tttgctttaa gaatatttag atttttatct tgcctaaagc tgccaaaacc ttttgttttc
     9121 tcgttaacac aaataaaaag gcaccgcgct ttatgtacgg tgcctcaatt ttatttagtc
     9181 tgccttttga taaatcctta catagtcgac aataaactct gttgtaaagt ccttgctgtg
     9241 atttatctcc tgcggcacgc agagagaaac tataacctgc tcgggaacct ttgatgtgcc
     9301 gtcagcaaag gaggtgcggg cggtttcaac accgtttaca tagaagatgt actcgttttc
     9361 gtcccactta agaccgtagg tgttgtactc ctcgtaaatg ttgtttccgt agaacttgcc
     9421 taccgccagt ttgttaagac ccgttccgtt tgtcgggtcg ccagtcattc cgctgaccca
     9481 tattgtctgg tgaattccgt ttctctccaa gaagcttgag ttataagaca ttgcctccat
     9541 aatgtctatt tcggctccgc ccactccgcc tctggaaatc tcggggttgt aggaatagtt
     9601 agactgcagc cagaaggcgc tccagaagcc gccgccggga ttgcatatgc agcggatttc
     9661 aaagtagccg aacttataat cgtcttttac cttgaccatg ccggcgtacc agccgggacc
     9721 gaatttgccg ttttcaaggt actcgccttt aataatcata ttgccgtttt cgaccctgac
     9781 ctgatggtcg gcggagaaac ctccgcgctg cctgccctct cccctgtggg tccactttgt
     9841 caaatccaaa gcgtcgcctt cgaactcgtc ctcaaagaca agctcatagc ccgtaaggtc
     9901 caattcaggg ccgctcggag tcatgggcag gtcgaaaaac agacacccaa gaacctccgc
     9961 gagtaccact aaagatgcta ttaggttttt gccgttaata atgtcccata atgccttttt
    10021 acctttgtta agagcctgaa cctgccgcca ggcaagtttt tcaagcagca tagctatgcc
    10081 cctctcccgt ttgttttgta atattatacc atcgggaaac ttgaggggca agaaaaaaac
    10141 acggactttt gtagtttttt tctaagttat gagccggatt ttgagtcttt tttgtaaaaa
    10201 gcgatgtttt tattcctgtc gcagacggcg aggaaaactt ctgacgccga ttttatgtta
    10261 tgctcatcca tcttttttga aagccaagtt aaatcaagcc ccaattgctt taggttcttt
    10321 tccataacgc gtccgtccat aattatttcg gcaaagaggc tttcctgagg caccttaatc
    10381 tgcaaatcat cgggcgtcac agaacggtgg gagctgaagg gcagaatagt cagtttaccg
    10441 ttactctcaa acaatgcggt gtgaatgttt ctaatatcaa aatagccctt ctccctgcac
    10501 atcataagga attcattaag gtccagcttt gcttttttaa gattctctct aatgagcttg
    10561 ccgttgtcaa tgagctttac ggacgcgccg tttaagtact ccctcgcctt gggtattttt
    10621 cttgtaacga cacctataat aatcgtcgca agagcgtaaa taaccatcgc aacagtcgac
    10681 ttccacgggg cttcaaggtc tgtcgcgagc tccgcggcga ttgagcctat tgtaattccg
    10741 gttatatagt cgaaaaaatc aagctgagaa acctgcttat gtcccatcag ctttgtaagg
    10801 ataaacaggg ctataaaaga aaatacgctt gtcagaatta cctgtagaat atccataccg
    10861 caccttaata gaaagttctt tacggctatt atttgcatac tgctgtgttt aattcattta
    10921 aggcactgaa ggctcggcgg cttggaatat ttaagtctat taggcaaaag atattagcga
    10981 aatctatata gaggggttcg gcagatgtta ataattgtaa ttagggcttt ggtcatcttt
    11041 gttatactca atatcctttt aaggattatg ggaaaaaggc aaataggaca gcttcagccc
    11101 attgagcttg taattacgat acttctttca gaaatatctg ttcagccact gacgcagtac
    11161 gaggcttcat tcgtaacgcc cataatcact cttgttattc ttgtaagcct agagattata
    11221 gcctctaccc tttgcatgaa aagcgtaaca ttcagaaatc ttatgcaggg caactcgctt
    11281 attatcataa gaaacggcgt tttggaccag gcgcagataa agcggctgag atttaccctt
    11341 gacgatattc ttgaggcgct gagaaaaaag gatgttttca gaatagaaga tgtgcagtac
    11401 gcgattgccg aaaccgatgg cagcctaagc gtattgctca aacccgaaaa aagaagcttt
    11461 acaaaagagg acggcaaaat taacgctccg gattacggca taccatccgt tgttgtatcc
    11521 gacggcaaga taattcagtc cgactttaag gaatgcggca tgacatatga aaagctggtt
    11581 caaaaactga attccttagg caaaagacct gaagacatta tgcttatgac catagacaag
    11641 gcgggcaatg tatttgtaat tgacaaggag aagaatatat gaaaaggttt tatatttctc
    11701 tcgccatgct tgcgtctgtc atcgccttca gcatatacgg ccatgtgact ctttacagga
    11761 attttgaaag gataaacgag gacttaaagc agtgcgttga agctcttgag aaagaggatg
    11821 aacaaggagt ttttgagcat ttaagcaaag ctgaaaagac atgggacgaa agcaagtggc
    11881 tcatcggctc cgtttacagc catcagacta aaatcgagat gaggcatttg ttccttttga
    11941 ttcgcgagct tgctgagtcg ggggacaaag agaactttaa agagaaaacg gaagaaatga
    12001 ttgcttatat tcaaacttcc attgacagca ttgcggtaag actttataat attttttaaa
    12061 ttctcactta gaaaacaaac ggaaaattta agataaataa taaaattacc acttgcattt
    12121 tataaatata ttttttataa tataaaagct gttcgacaca gcgcggcagt ttaataccaa
    12181 tgcggagaag tcgcctagtc tggtcgaggg cgcgcgattg gaaatcgcgt aggcgtcaaa
    12241 agcgcctcgg gagttcgaat ctccccttct ccgccacttt aaactgaata attaaagtta
    12301 attcactaac tggagaagta ctcaagatgg tgaagaggcg cccctgctaa gggtgtaggt
    12361 cgggtcacac cggcgcgagg gttcaagtcc ctccttctcc gccataaaaa acatcagcat
    12421 tgatttaatg ctgatgtttt tctttttgct gagcttggct gcaagaagga ttgcagaaag
    12481 gatttatata ggttttgctt atcttatctt tttggtttct atattgctga agcaagttat
    12541 atacggaaca ttaccggtat aaaggctcac agttatagta tttaaaaatt gttgacattt
    12601 cttaaattta aatttaaggc tgcaaattaa atcagctttt ctgcccatcc gcagtttata
    12661 taactcctga atttgttcgc aaataaaaaa cggaggtaaa gctgaattgc ttatacctcc
    12721 atacagtgct ttttcacttt tttaaccaag cgcgacatca agaatcatca tcaatgtaaa
    12781 tcccagcgcg acgccgattg taccgacatt gctgtgctcg ccgtcctgag cttcaggaat
    12841 caattcctca acaacaacat aaatcattgc tccggcggcg aaagccagga tatacggaag
    12901 aacagatgta acaagcgagg tcaggaaaat agtcaaaatc gcgccgatgg gttctacgac
    12961 accggaaaga aaaccgtatg aaaaagcctt gcgcttagag attccggtgg cgaccagcgg
    13021 catagagata attgcgcctt caggaaagtt ctgaatcgca atacctatcg aaagcgcaag
    13081 tgcggctgct gccgaaatgt tagcgttgcc ggaagagaat cccgcaagta caacaccgac
    13141 tgccattcct tcgggtatat tatgtaatgt aaccgcgaga atgagcatca gtgctttgct
    13201 gagtttggat ttgtgtcctt cagctttgtc cgtattaatg tgaaggtgcg ggatgagggt
    13261 atccattaaa aggagaaaac caatgccaat aagaaatccg acggcacacg gaatccaagc
    13321 tacgccgccc tgctcttccg tcatttcaat agcgggaata agaagcgacc aaacggacgc
    13381 cgctatcatc acgcctgacg cgaatccgag caaagctttt tgtacttttg gcttcatttc
    13441 atttttcaga aaaaagacca tagccgcacc gaggattgtt ccgataaggg gaagcagcag
    13501 tccagctaca agtatcattg tgtcactcat agggtcatcc tttcatgttt aaatcatggt
    13561 gggcttttta ctgcccgagc gtgtcgggca agtttccatc gtcatttgtt cattcatttt
    13621 tatattatat tactatttgc gtcatttcga caatactttt gcctttaatg aagtaaaagg
    13681 tatcctttaa ttgggttttt tttgctttct cccgcctatt cataatttat taaaatttta
    13741 tttgacaata tacaccagcg gctgtatatt caaattggga aaattaaacg cccgttttta
    13801 ggaggataca tatgaaaaag gcaaagaaac tcatttctgt cttgctcata ctttcaatgc
    13861 tgcttcctct gtgctttacc gctaacgcgg caaccgtaac ccagctgcca tttcccgaca
    13921 gcgcattttt tgaaataggc gattactcca tacattacag attcagaccc tctgccgcat
    13981 cagagcataa aggcagattt gtattcctgc acggcttttt aggctctacc gtatcatgga
    14041 aacccttgtc cgaccgtctt attcaagagg gctacgactg cctgcttgtg gacctgccca
    14101 gcttcggcta cagcacaagg gaaacagcgc aggttcatcc tatccccagg gaggagattg
    14161 ttgcttcgct tatgcaggaa ctcgctcccg cagagaaatg gtatgttgcc ggtcactcaa
    14221 tgggcaccac agtcgccatg aatattgcct gccggtatcc cgagcttgtt gaagcgttac
    14281 tgctctacgc cgccaccagc gacaccaaga ccaaatttac gaacctcccc gaccccgtac
    14341 ttcaggtcta tggcggagtc tataatgtga tatttaagat gataattact ctggcatcaa
    14401 atcctgtttt cggcaaaatt ataatgagga atgctctata tgatgccaac agggattttg
    14461 agacaaggca gaaatacctt gagccgttaa agcttgaaaa caccggcatg ggaatcctct
    14521 ttatgagaaa acgctcaacg ccgctggatt ttgagcagtt aaagcagctt gagatgcctg
    14581 taatgctctg tgtcgcaagc gatgatgaat ttgttccgaa tgattcggag aatgcgataa
    14641 gactcagaaa ttccttgccc gctcatacgg tctattatgt tatgcccgat tcgactcatt
    14701 cattaatcga atccaaagct gacgaaacct ttgcggtgac atttgatttc ataaacggca
    14761 tactgcatta atacataaaa caataagctc acctttccgt gaacgcaaaa tcggcatagc
    14821 tttggttaac ggctatgccg attttctatt tgttgttcta aaaatactta ttgttaataa
    14881 gcttatcttg ccatataatc aaatagtaat ctctgccaaa aggagaaatg aaatggcaaa
    14941 gcaggcacga atgattatac ataaagagta taaaaaggga gatatagacc ctaagattta
    15001 cggctccttt attgaacatc tcggcagggc cgtctatagc ggaatatatg agccggggca
    15061 tccgacagcc gatgaaaaca gcttcaggca ggatgtgctg gagcttgtaa aaaagctgaa
    15121 ggttcccgca gtgcgttacc caggcggcaa ttttgtttcg ggctaccgct gggaggacgg
    15181 cacgggagat aaaaagaacc gcccagtctg cgcggacctt gcttggcata ccattgaaac
    15241 caattaggtt ggaattgatg aatttcaggc atgggccaag aaagcggaaa cagaaattat
    15301 tatggctgta aacctcggca caagaggacc cgaagacgcc cgcaatctgg tggagtattg
    15361 caacggcaca atgcctacac agtatgccga aatgagaagg aaaaacggat ttgaggagcc
    15421 tttcgccatt aaatattggt gcttaggcaa tgaaatggac gggcactggc aaatctgcca
    15481 caaaaccgct gcggaatatg gcaggattgc tttagaagcg ggaaagctta tgaaaatgat
    15541 tgaccctgac attaaacttg tgctctgcgg cagctccaat tacaacatga gcaccttcgg
    15601 cgactgggaa tggacggttc tgaatgaggc gtacagcgtc gtcgactata tttcgctgca
    15661 tcagtattac tcccgttccg aatttaaatc aaccgaggat ttcttgggca gagcttctca
    15721 catggactca tttataaaag gcgttgccgc catttgcgac gctgttcagg caaaaaagca
    15781 ctcaaagaag aaaatccacc tctcctttga tgaatggaat gtttgggacc agagggtttg
    15841 gggcggcgac cccgagccgg gtgagccctg gcagcaaaag ccgcataaac tggagcagat
    15901 ttataatttt gaagacgctt tgctggtcgg cactatgctc tctactctcc agaacaactg
    15961 cgacagggtg aaaatcgcct gcatggcgca gcttgtaaat gttatcgccc ccattatgac
    16021 tgaggagggc ggcaaagcat gggtgcaaac tattttctat ccctattatt acgcatccgt
    16081 tttcggccgc ggcgaatgtc ttgacgcaaa ggtgtactct gacagttacc gcacttccga
    16141 cggatataat gttccttatg tcaacgcttc ggtggttttg tctgagaaga aggacaaaat
    16201 ctatgtcttt gcaagcaatc tctctttgga agaggatatg gaattaaccc ttagctttga
    16261 ggcttttggt gaaataaccc caatctcaaa aacagagctt tactgtgatg atttaaaggc
    16321 tgtgaacacc aaagactttg aagcggtatc tccaacaacc gttcagctta acggaaagag
    16381 tgatacttcg cataccttga gtctaaaaaa gcattcatgg aatatgtttg aatttaagat
    16441 tcaataaata agcaaacccg ttagcctgac atttggctga cgggttcttt tgcggtatcg
    16501 actgttcgtg aggtactttt gcttatctga cagttagtaa atataaaagc agcttagcaa
    16561 atctgccgtt tattttctta taagttctat gccctttttt aaagtatctt catcaattgc
    16621 gcctatcgct ttagttacta tatatccgtc tttatcaata aacagcgtca tggggatgga
    16681 tgtaatgccg taggctttgg cagcttcctg atacatatcg aaatacacgg ggaatctgta
    16741 gcctcggctt gttataaact ctaagccctt ttccctcgtt tccctgtttc cgtcaacaag
    16801 gtcaaccatc ataaaggcga cggcggtgcc gtattcttcg taaaccttat tgaactccgg
    16861 catttcctct ttgcacggag aacaccagct tgcccagaag tttaccacta cgggtctgcc
    16921 aagcatatcg gacagcttta catatttgcc gtcaatgttt acaaccataa aatcttcggc
    16981 ttttttaaga ttgcctttac cctcttttgt ctccgccccg atttgaataa ggtcctgatc
    17041 cggggcgtat tccctcgtca gctcattgta caaaaatgcg ctcatggcga taatcagagc
    17101 aaagaaaacg attaatagaa cagtgtttgt ttttttactc attttcagtc tcttttaaaa
    17161 agtcagcaga gaaagaaatc tgcccataat tcctgtagcc atcaaaatgc ctacaattat
    17221 taacagcacg cccgacagcg tgttaataac cttatagttc ttctttataa agtcaaatgc
    17281 cgttttaagt ctgtcgagaa gtatagcgct catgataaaa ggaagaccga gacctaaaga
    17341 aaaagcaagc agcattgtga ttcctttaaa ggcagagccg ctgacagcag ccatcataag
    17401 agcagagcca aggaacgctc cgacgcacgg agtccatccg attgaaaaga ttataccaaa
    17461 aagaaggctt gagaaaaatc ccagctcttt aatctctctg tcttttcctc cgctgcggtt
    17521 taataatcct attttcagaa ctcccaaaaa attaagacct aaaacaatta ctataagacc
    17581 tgtgactata tttacaaccg tcgaatagct ttgaagcagc ctgcctacag tgcccgcaaa
    17641 ggcgcccatg gcgatgaaca ttgcggaaaa gccagaaacg aagcctattg agttaataag
    17701 ggcttttttg cccgccgcct gccctgccgc gaaatatgaa acatatacgg gcagcagcgg
    17761 caaaatgcag ggtgatatga atgttattat gccctccaaa aacaacagca gatactgcat
    17821 aatttgtctc ccttttatgt ccgaacctca acagaactca ctatgcaaat gcaatctcag
    17881 ttacaatagt gcttttttaa tacatttatt atttcctcga ccctgtggtc ggcaatccaa
    17941 taagttatgt tctgtccgga cttttcaaaa tccaagatag agtgagcttt taatatcgca
    18001 aggtgctgtg acaacgccga ctgggtaatg ttcggtacct tttcggagat tctgcttacg
    18061 gttttgggcc cctccataag ggcgcacaga atcataagac ggttatcgtt ggcaagcacc
    18121 ttcaaaagct ctgcaatttg ttttgctttt ttctccacta actgactccc ctttgttttt
    18181 aatcttacat ataccttgtg tcatagttcc cataggacaa tagacgcacc aggtacgcgg
    18241 tttgaaaatt atcattgtga ctaaacctaa aactgttgaa gtaagcatta cagagtaaaa
    18301 tccgaaggca aactgagcga ctaccgcggg gacgaaggaa acatctgacc aattccacgg
    18361 cagcttaaag agccaaagca aggtaacggc ttgttttagc ggcgcacccg caaaaacaag
    18421 atatgtggaa taaatcatca taaaaaacat acttaaaaag aaaactaaaa agccgtacct
    18481 aaaaatagat gacctgagaa atttgggcgg atctttttta aaggaaattc cgagcttatc
    18541 ccccaatatc gtgaaaagct gtccccgtcc gcagtatttg ttgcagtaag atttatctcc
    18601 tcttattatg gcaatcatta gcggaacggt aaagcataat aatccaatat atgcaaacaa
    18661 aatgttaaaa aatcctaaaa aaaggtaaac aagtgagaaa atccacaggt aatcgtacca
    18721 tcttttggtt ctcatttggg attttgcctt aggtttttgt gttcttctct cccgcccctc
    18781 tatggagatt acagcggccg gacacgcctt tgcgcatttt ccgcagccaa tgcagatttc
    18841 ttcatccacc actgcggtaa ttcctttatt aatcgaaatc gcgttcatcg ggcagacctt
    18901 taagcaggtg ccgcaggcta cacagtcttt tgaaacatga gcgtacttta tacttgacat
    18961 attccacctc agctcaatat attagtatat tcttaaatac taatatatag ttatgccaaa
    19021 agcatgtcaa gtgttcaacc aaatattgca aagaatactt gaaaataaaa aatcgggcat
    19081 aataagctca aaggagtgtt tttatgaacg gtaatgcaga gcttttaaac tttatttatc
    19141 agaactctca gatgggcgtt aataccttga gccagcttat ggatatagct gaagatgagg
    19201 agttcagaaa gcatcttcag cagcagtacg aggagtacaa ggacattcac gaaaaggcaa
    19261 agcaggctct taatgaaaac ggctatgacg aaaagggaat aagcgccctt gacaagatta
    19321 agacctatat tatggtcaac attcagaccc tgactgacaa atcctcatcc cacatcgctg
    19381 aaatgcttat ggtgggcagc aatatgggta ttattaatgc catcaagaac cttaagaaat
    19441 acagcgatgc agaacaaagc attaaaagcc ttatggaaag gcttttaaaa ctcgaagaaa
    19501 acaatgttca gcagctgaaa aacttcctat aaaaaagccg ccccttaggg cggttttttt
    19561 atacccattt tcgcgctccc acaatgtgaa gaaagcgcat accatccgcg ccgacatagc
    19621 tgtcaagcgg tctgttgagg gcatcgtatg tatgggcgga aatcagaata ttgccgttat
    19681 tgtttgacat caccacaagc gaatgatagt aggttcctga gctgtctctt agctgaatta
    19741 tatcgccttt ttgaatgtgc tccctgtcgg tttcgacgca gtagggaccg acttctttgt
    19801 tgtttataag gaatttatac agatactgaa cagagctcca agcagggctt ctgttattaa
    19861 gattaatgta ataccagccg taaatcggcg tataattcat tactctgcag cctgcgtaaa
    19921 tgcactggga aacgaaattt gtgcagtcgc cgcccatgcc gtcaaagtta gcgtagcgtg
    19981 gatttctgct cagcgcccat tttgccgcgt actccaccac cgcgtctcag ttataaggaa
    20041 ctgatacaag cataatatca catcattcga atattttttc tcgttatatt ctatgacgcg
    20101 aaaaaaacaa ggctacaaaa aaggcgcagg atttaacctg cgccttgctg tttttatttc
    20161 tttagtttct gttcttgaac atatcgaaga tatcggtaag gtcgtcatcg aagccgaatg
    20221 cgtcgccttc cgcctcttcg gagcctgcag ccttaattgc gttgccgtcc ttgccaaagc
    20281 ctgttgcgat gactgtgacg ataacctcgt cgtcaaggtc gggattaatg gcaacaccga
    20341 agatgatgtt ggcatccttg tctgccgcgt tggagattgt tgttgccgca gcgtcaacat
    20401 cgtcaagagt gatgtcgggc gaagcggtga tattgatgat aacacccttt gcgccctgga
    20461 ttgttgtttc aagcagagga ctggagatag cagcgctggc tgcctgaact gccttgtcct
    20521 tgccgcttgc cttgccaaca cccatgtgag cgtgacctgc gtccttcatg acggaggtta
    20581 catcggcaaa gtcaaggttg ataaggcccg gaatcttaat aagctctgaa atgctctgaa
    20641 cgccctgcct taaaacatcg tcggcaattt caaaggcgtt gaggaatgtg agcttctggt
    20701 cggtaaccag cttaagtctc tcgttgggga taacgataag gctgtccaca tgctgagcaa
    20761 gctcggagat gcctgcctct gcctgctgca tgcggcgacg accctcaaac tcgaagggct
    20821 ttgtaactat gccgactgta agtataccct tatccttagc catgcgggca acaacgggag
    20881 cagcgcctgt gcctgtgccg ccgcccatgc ctgctgtgat aaataccata tctgtaccgg
    20941 caagagcctc ttcaataagg tcagagctct cctcagcggc ctttttgcca acctcgggac
    21001 gagcgcctgc gccctgaccg cttgttgtct tctcgcctat ctgaatttta tgtgtagctt
    21061 tcgaattgga aagaacctgc ttgtccgtgt taatagcgat aaattcaaca ccctgaacat
    21121 tcgcgttaat catgcgatta atagcatttc cgccgccgcc gccaacgccg ataaccttta
    21181 ttttggtcac ttcttcaaac tcattagata tctcaaaagc cattttacat cctccgttga
    21241 tatgatatga ggcttacacc cttattacat acaattaaac acagatataa taacatttat
    21301 tttcgcaaat ttcaacattt gaaactatat ttaacacaaa tttagcaaaa ctttgtatta
    21361 aacataaatt tttatacagt tttttcctaa cggtatatta ccatattcga atgttaattt
    21421 caaactcttt cagtctgctt ttccgatgtg ctttcactct cgctttccgg ctcttttgag
    21481 ggctccggct gtgtttcggc agaggttgta atatcacgca catgagcctc acgcaagatt
    21541 ctcaggtcaa tgtaaatcgg gtttacaatg ttcttttcct tttcggcggc aattacggac
    21601 ttcgcaaaac tgagcttctt gtcaacatcg tttatgctgc ccaaatccag cctgataatg
    21661 tcctctatcc agacccgcag gtcaacggta tcggtcaaat caatctcggt gattttctct
    21721 aaaccgtttg tttcgattga ggtcagaagc tgaattaaaa gctcatagcc gccatcttcg
    21781 ttaaactcag caatactgcc cagctcgggt tctttgaggg ttaggcctct gaccagagcc
    21841 gtggggtcgg gcggaattgc catacctgtg gtgccgagga ttttggcgtt cttgcttaac
    21901 aggtagtatg tatctcctgc ctcgacagcc aaggcaggta cggcgtccgt cgtttgaata
    21961 attattttgt tggggagaga aattttggtt ttcgctgatt cgatatatgg caaatccttt
    22021 tctatttttg cggatacatt ttttaaagac tgcaaaaaaa tattctcgcc gagctttatt
    22081 cccgatgcct caataatttc agcctcggaa taataaaggc tgtcggtggt tgctgtgatg
    22141 acttcgatat ggaaaaagac agtcagggac aaaacaattc ccgcgccggc tacaaaaagg
    22201 gcaaggaata tgtaaatcat tctatccctg cgccttttct tcttgcggcg tttttcgtta
    22261 tcccttatta ctctttcggt gtttgaaagc tgtctgtcgg gaacgctgtt ttttctttgt
    22321 cctttcatat ttttctgtcc tttcttataa gagggcagct aataagaatt gttttcatca
    22381 ttcaactcgt tttatattgg cacccattga gcttaaaaca tcctcaagac cgtcgtagcc
    22441 tctgtcaata tgattgatgg cgtcaacaac ggtggtaccc ttagcagaga ggcccgcaag
    22501 aacaagcgcg cagcctcctc tgaggtcggg agacacaacc tctgcgccgg taagacggtc
    22561 aactccttct acaacggcgg ttctgccgtc aaccataatt ttggcgccca agcgcgcaag
    22621 ctgacccaca tgcttgaatc tgttttcaaa tatggtttcg attattacgc ttgtgccctt
    22681 tgccactgag gtaagcgcca tcacaggtgc ttgcaaatcg gtcgggaaac ccggataggg
    22741 catggtcctt attgtcttta ccctttggag tctggaaggc gcgacaaggg taagatttct
    22801 gccgtttatg ctgatgtcgc agcccatctc ctcaaaaaca gagattaccg aaccgagatg
    22861 cgagggaata atattgcgaa gtgttacact gccttgcgtt atggcggcgg ctcccatgta
    22921 ggtcgcggcg gctattctgt cggggatgac ggtgtgctct gtacccgata gggattttac
    22981 gccctctatt acaaccgtgc tctcgcccgc gcctgaaatc cttgcgccgc agccgttgag
    23041 gaaatccgcc aaatcgctga tttccggctc ccttgctgcg ttgattatta ccgttgttcc
    23101 cactgcggtt gaggcggcga gaataatatt ctccgttgcg ccgacgctcg ggaacgacag
    23161 cgtaattgtg gcagccttta atccgttttt cgcagtgaat tcaagccttc cgtggccttc
    23221 ttcaagctcg acgccgaatt tctccatagc gtcaagatgc aggtttatag gtctgagacc
    23281 tatttcgcat ccgccgggag tagagatatt cgccttgccg agtcttccca gtacggctcc
    23341 caaaaagata atcgaggagc gcatctcgtg catcaggttt tcgggaatgt cgtagcgggt
    23401 gatatctgtg ctgtccacta tcaccgtaga gccttccctt tttaccttgc agccgagatg
    23461 ttctaaaata ttgattgtcg cagcaatatc caacagccgg ggacagttat gtataaccga
    23521 tactccctta cacagaactg tcgcggccaa aatgggaagc gcactgtttt ttgagccttg
    23581 aacatcaatt tcgccacgga gctttctttg accttctata atatatttcc ccatcttata
    23641 caccctttct gatgaaagaa acgatcgtct gctttcatat tatgaagaaa gggatgttct
    23701 ggtgaaattt caagtccagt ttttaaaggt ttggttttaa gactttaaat ctggcctttt
    23761 gacggggaag tttacgaaaa aattgatatt aggcttgtcc aaaattcaag cggaaaaaaa
    23821 ttatttttcc acgagcttta aaatttcgtc gcagatacgc tctgtggcgt cggttacagc
    23881 catctttttg gcgttggcgc cgagctcatc tcgtctttct ttgtcctgaa ccagctcttt
    23941 tatgaactgg acaaatttct tcgcgtcgag gtctttttcc tcaaagagaa ccgcggcgtt
    24001 tttgtcggca agagcctttg cgttgtaata ctggtgattt tccgagacat tgggagaggg
    24061 aataagtatt gccgccttgc cgagcgcctg aatttccgct atggcgctgg ctcccgccct
    24121 tgatacgact atatccgctg cggacaggca gacatccata ttgtaaatgt actctcttac
    24181 atctatgttt ggggaattgt ctatgtcgat tcccctttct ttcagctttt cggggaacca
    24241 atgaccgtac tgaccgtgac cgtgtataaa tcttaaattc ttttccttat gaagctcggt
    24301 aataacctca agcataagct catttatggt tctggcgccg aggctgccgc ctgtggaaaa
    24361 aatcagaatc tcattgggct taatatgcag ctcagcgcgt gagaaatcct tgttcgcgca
    24421 aagaatcgct cccctgacgg gaagacccgt tacaacgggg ggatttttgc attttaatct
    24481 ctccgtcgcc tcgggaacgg cggtcataac agtatccgca tacttcgcga gagtcttgtt
    24541 tgtaatgccg gggaaggcgt tctgctcatg tattgcggtc ttaatgccca ttttggcggc
    24601 ggttcttacg accgggccgc ttacatagcc gccaaagcct attacagcgt cgggcttaaa
    24661 ctctttgagt atctttttta cctgcgagcc cgaggtaata agccttttta aggtgattat
    24721 gtttctcttt atgttctgcg gcgtgagttt tctttgaaag ccgctgattt cgatagtctt
    24781 aaaatcaaag cccgcgccgg ggacgagctt tgcttccatt ttgtcttttg tgcctataaa
    24841 aagaatatcc gcatcgggga atctccttct aatctcaccc gctgcggcaa gcgcggggtt
    24901 aatatgaccg cctgtacctc ctgcggcaaa tacaactctc attttgcatc tccttaaaat
    24961 gcaggattag ttatttttct tgagtgaggt atatctcgaa accgatagaa tcatacccat
    25021 ttcagcgagt agaatcagca gcgacgaacc gccgtagctg aagaagggaa ggcttattcc
    25081 cgtattgggt atgctgtcgg tggcaactgc gatattgaga accgtctgaa gtcctacctg
    25141 gaatacaatg cccattgaaa gcagcgcgcc gaatctgctc tttgatttaa gtccgataac
    25201 gacgcctctc caaacgagaa ccgcgaaaag gaagagtata ataacggctc caacaaagcc
    25261 cagctcttcg cagacaatgg agaaaatcat gtcgttctgc ggctctgaaa catacatata
    25321 tttctgcttg gaatttccga gacccgctcc gaaaaagccg ccggagccaa tggcgtaaag
    25381 cgcctgattt acctgccatc ttgcgcccgt gggctcaaag tccttgtcga gccatgccac
    25441 aattcgctcc gtcatgtatt cttgcaagat attgggtcta aggctcggga acattacaaa
    25501 gacggcgacc acaaaggcga caaggagaaa tcccgcaata aaccagcgcc tgtcaaagcc
    25561 gccgagatac agcattataa gtcctatgca aagcattaga attgtgcccg aaaggtgatt
    25621 ttcgaggaat acgagcacgg cgaaaacaag gataattccg ccgtacaaaa caagggacgc
    25681 aaaggactcg gttattctga ttctgccgtt tgttatgcgg ttaagagaat gggcaaacgg
    25741 tgcttttata agctcttttg agttaatctt cttatagttc ttatcgaact gccatgcgca
    25801 gaacattata agtcccagtt tcgctatttc cgaaggctgt atggtaaagc ccgaaagtcc
    25861 gggtattgtc agccatctgt ggtgaccttc cttaaccgcg ggggtaaaga gaaccaaaac
    25921 caataggaac agggacatga agaatccgat taatgccgcg agtctgaagt attcgaacct
    25981 tactcttgac agcacaatca ttatcacaaa gcccattacg gcaaagataa gctgcctgta
    26041 gaaaaagtgg gtggaatttt tatatttttg cagagcgtaa ggatagcttg cagaaaagag
    26101 cattacaaga cctataacca aaagaagcgt tacggtaata aagaatacaa ggtcgaagct
    26161 gccctcggca accgccattc tgattaggct ttttcttctg cgctttactg ctttttctgc
    26221 caagccgacc acccctaaag gcttattttc gtttaagtat ataacaaaac gctacttaat
    26281 tctgccgaac catgtaataa gaattgagaa aagtccgcca agggctccta taaggctgaa
    26341 aacctttaca atcttgacct cgctccagcc gcttttttca aagtggtggt gaatgggagc
    26401 catcttgaat atgcgctttc cgccggtgag tctgaagtaa acaatctgaa tgacatcgga
    26461 aaggaactcg ataacataaa taattccaac cgtaagcaag agccacgggg cgtcaagtgc
    26521 gtaggatatg gcgattacca taccgccgag gaaaagggag ccgacatcgc ccatcataac
    26581 ctttgcggga tgccagttcc agataagata gccgatgaga gaaccgaaaa gcgcgctgga
    26641 aataaggctt acgccgaaca ttcctctgag taccgcgatt acggcaaagc tgacacatgc
    26701 aacagctgta acggaggcgc aaagtccgtc aattccgtcg gtaaagttaa ccgcgtttac
    26761 tgtgcagtag attgcgagaa cgcccaaaat ccagtagaaa attccgatgt ccctgactcc
    26821 cacaaaggga atataaatat aggtcgctcc gactgagctt aaagtataga gatataaacc
    26881 catgattata acctgggcaa tgctcttttg cattattgta agacccagat tcctgcccct
    26941 tttgaccttg atatagtcat ctgcaaagcc gacaagggca aaggctatcg ccatcaaaag
    27001 tccgccgaaa atttttacct ttacggtatt cggcacaacg gcgtcgcccg agattatatc
    27061 gtctcccata aatctatagg tcagaacggt aactataagg gaggcgatta ctcccgctat
    27121 aaagagtatg ccgcccatgg tgggggtacc ctgctttttc ttgtgccatg agggacctat
    27181 atcaagaatt gtctgaccga atttaagctt atggagccat gggattacca catagccgag
    27241 ggcataggct gtgccaaagc ccacgagcgc tgataaaatc agagcgaacc acttattcat
    27301 tatatcctgt cctttccgaa cttatgccat aggcaaatgc agattggtaa ttatttgttt
    27361 actctccgag taaatcatac agcttgttca gtacattttc cagcttcatt cccctgctgc
    27421 cttttacgag cactccgtca tttgaacgaa ggatactata aagcttttcc gcaagggcat
    27481 cgtggctgtc aaaagtataa acctcactga cgccggaatc aatagctgtt cttgctgtat
    27541 tctttgaaag ctcgccgtat gtgaacagaa tatccacatt attgtcctta gccgatttgc
    27601 cgattgattc atgagccgat accgcaatat cgccaagctc cagcatgtcg ccaagaacgg
    27661 cgattcttct ttttacagtc atatttttaa ggacgctgag tgacgcgttg accgaatcgg
    27721 ggcttgcgtt gtagcagtct tctataaagg ttatgccctt tacgactttt acacgctggc
    27781 gcataccgct gggctcgtaa agctgaagag ctttacacat atcttcaacc ggaatgtcaa
    27841 agacctttcc agccgctaaa gcaagaagag cgtttataat attgtgcctg ccggctgcgg
    27901 ggagctttac actgcccttt tcgcccagga catttacctg gaaatatgag ccctctgcgg
    27961 tttcaataat attattcgca tagacatcgg cttttttctc catggagcac ctgattagtc
    28021 taaagccttt aatatcaaga cctgagagca tatcgttgtc cgcattgatg ataagcgggc
    28081 tgccctcttt aagaccgtca aggatttcga gctttgcttt gaggatattc tcccttgagc
    28141 cgaggttttc tatatgtgta acgcccacat tggttattat tgccgcgtcg gaggctgcta
    28201 ttttgctgag gagtgaaatc tcccccgcat ggttcatacc catctccaca acggcggcct
    28261 gatgacaagc atcaatactt aacagcgttg cgggaacgcc tatatcatta ttgaggttgc
    28321 cttgagtcct gaaggtatta tactttgcgg aaaagcaaag gtaggtaaag tcctttgttg
    28381 tggtttttcc cacgcttcct gtaatgccta ctacctttac acctgttttt tgtctgtaat
    28441 aggctgcgag cttttgatat gcggctttgg tgtcgtccac aaggatataa ggaacatccg
    28501 catccggcgc ttttcggcac aaaaccgctg cggcaccttc ttttaccgcg gcaccgataa
    28561 agtcgtgacc gtcaaaattc tcaccttcaa tagctacaaa cagaccgcct tttaccggct
    28621 ttctgctgtc ggtgaaaact ccgtcaatgg ttatatcttt ttccgctttg ccgccaacgg
    28681 cttttgctat ttccttagtg ccgagtttaa tcaaaggatt tcctcctgta ctttttattt
    28741 catttcttca agcagttctt ttacaacctc acgctcatcg tagtgaatgg ttttgtcttt
    28801 taatatctga taggtttcct gacctttgcc cgcaagaaga actatatccc ctgcctttgc
    28861 ctcctttaag gcgatggcgg tcgcttttct tctgtcaggc tccacgaaaa ctttagcctt
    28921 tgtattctta atgccttcca gaatatcatc tattatcttc tgcgggtcct cggttctggg
    28981 attgtcggat gtgaccacca caacatcgga gagctggctt gccacatcgc ccatgatcgg
    29041 acgctttgtc ttgtctctgt ctccgccgca gccgaaaaga gtgattattc tgcccgcggc
    29101 aatctgccta agcgcagtaa taacatttac aagtccgtcg ggtgtgtggg catagtcaat
    29161 aattacggta aagtcggtat ttgtgggcac gatttctact ctgcccttta cgccctcagc
    29221 cttttcaaga gcttcaagaa cttctttcat gccaaagccc aattcgacaa gggtcaccgc
    29281 cgcggccatt gaattgtaaa cggagaactt gccggggatg cccacatgag ccctgccaat
    29341 gtctcctttt gttacaagct catatgaaac ggcggtctgg cgatactgta tgttctttgc
    29401 ggtaaaatcc gcagcgtcgt cctctattga gtaagtgacc acctttgaat ttgagccttc
    29461 cgccatgtgt ttatagtaac tgtcgtccat gttaagcacg gaaatatcgc acattctaaa
    29521 gagtttttgc ttcgcgtctg cgtaagcctc aaaactgccg tgataatcca gatggtcctg
    29581 agtgagattt gtgaacacag cggctctgaa atggcagccc tctaccctct cctgagcgag
    29641 agcctgagaa gaaacctcca tcacgcagta ttcgcagttt tcgtccgcca ttctgcggaa
    29701 cagcgactgc aactcaaagg gttcgggagt tgtgagaact gcttcaagct ctgtgtcgcc
    29761 aatcatattc ttaacggtgc cgattagacc cgctttaatg cccagtttat ccataatgga
    29821 tttaattagg atagcggttg tgctcttgcc gttggtgcct gtaatgccaa tgagctttaa
    29881 atcttcggag ggatttgaaa aaaaggccga gcacataagg gaataggctt ttctggtatt
    29941 gtcgcaaata acctgcctgt caagacctaa gtcacgctca acaattaccg cagccgcgcc
    30001 cttttggaga gcctctgcgg caacattatg ggcgtcaaac ttattgccct taatgcagac
    30061 aaaggcgcag ccctctgtga ttttattgga gttgtcggta acagcggtta tttcggtatc
    30121 cttataatct gacaagacat ttaccttttt aagcagttcg gatagcttca aatgagtccc
    30181 tccaatgcct tttattcgtc cgagtctgaa acatcgacat ttgacttaaa gtaaaccgtt
    30241 acaacggtgc cgaggggaac cttttcgccg ggggctatgc tttgcctgta agcgaaaacc
    30301 tctccgctgt taagtgagct gcccgcaatc ttgacattga gtccgttatt aatggcagcc
    30361 ttgtttgccg cagataccgt catgccctta aagtcgggaa caacagcctc ctgagatgtg
    30421 ttctttgact ctgtgtaaag cacaaccacg ccctttttgg gtattgctct gtcggattcg
    30481 ggcgactgtg ccactaccgt atcgccgctg cctataactc ttgcggtaaa gcccgctctg
    30541 gtgatttcgt tctttgcttc gttaacgctc tttgaaacca cattcggggc gtagcttaca
    30601 agcagggcaa gctcgttgtc ggagtactgg ggctctatat tgaggtaagg cagaacctgg
    30661 tcaaagattt cgccggctac gggagccgca accgcgccgc cgccgtgctc gccaacaggt
    30721 tcgtctatag ccacaagcac agcgaccttc gggtcatttg cgggggcaaa gcctacaaag
    30781 gatgcgacat aagcgccgtc tttgccgagc ttctgggatg tacccgtttt gcctgccaca
    30841 tggtagccgg caacataggc gtttttacct gttcccgatg ttacaaccgc ctccaaatgc
    30901 tctcttatga tggcggctgt tttctctgag attacctgac gcttgactat ggggctaatc
    30961 tctttaataa cctttccttc agcgtcgatt tcctttgcga ccacataggg ctgcatcagc
    31021 tttccgccgt ttgcgaccgc tgatattgcg gtaatcatct gtatgggagt gacctggaac
    31081 gactgaccga aggaatagct tgaaagctga gatataccaa agttcttttt ggagtgatat
    31141 gttacgccct cattgggagc cgcctcgccg ggcaggtcaa tgcccgtctt ttcggtaaga
    31201 ccgaaggctt caaaatactc gtagtatttt tcggtgccga ccagcttagc aattgtaacc
    31261 gcaaaggggt tgcaggagtt tttaagaagg tcgggcagcc tttgcgagcc gtgaccgccg
    31321 tgcttccagc acctaatgat tctgtccgct actgcgattg agcctgtgca ggtatagccg
    31381 aaattgttgt taacaagacc ttcctcaacc gcagcagaga ccgtcacaac cttgaaaact
    31441 gagcccggct catagctgtc gcttatggtc ctgtttctcc actgggcgta tttggcgttt
    31501 aacagagcct cgtttctctc tttttcgtcc tgaatgctct caatttgctt tcttacattt
    31561 tcatctaata taaggtcggg attgttgggg tcaaagtcgg gcaggtttgc cattgcgaga
    31621 atcgcgcccg tcttgacatc cataacaatg ccgtaaacat tctttgcctt tgtttcctgc
    31681 aaagagtgat tgagtgcgct ttcaaggtag tactgaacga cttcgtctat tgtcagaaca
    31741 agatttgtgc cggggatagc gtcatatgtt gactcatact gattgggcat ggcctcaagt
    31801 ctgccgtctt ttgcggtgac taccctgccc gcggtgcctg taagaatgtc gttataaaga
    31861 tattcaagcc ctgctctgcc aatatcgtct ttacccgtaa aaccgagcac ggtggcagca
    31921 aagcctgaat agggataata tctctttgtg tccgggtcta tgtttactat gccggtcaat
    31981 ttgttttgct gaatgaactt taagacttcc tccattaccg gcttttcgat ttgacccttt
    32041 ataagctcgt agcccacatg ggttctttcg gccttctttt tgattgtttc ggggtttact
    32101 tcaagtatct gcgaaagacc gtcgataacg agctgttttg tggcttcggt tttgattttt
    32161 gaaggattta tgtaaaccag ccaaactgac gcgctttggg caagcacctt catattcctg
    32221 tcatatatgg tgccgcgctg ggcttttatt accgtatcgc tgagctgctg agcctccgcc
    32281 ttcgctttgt attcctctcc cttaacaagc tgcagatagg caagacgccc gcttaaagaa
    32341 agaaaacctg caactaccaa cagtgcaagt ataattaatc ctcttgttgc aaggagcttt
    32401 tgagttttca aaaaacacac tctccttaaa gaacctccgc cgcaggcttg gaagctcggc
    32461 ggcagaggtt attttccttt ttgaatgcaa gtcttacaac ccctgcatta taattattgc
    32521 agaattaaaa aatatatgcc aaaagctggc ggaaaattga gggctcctcg tttacctctt
    32581 tgccgcccga tactacaacc tcgtcgcctt tggacagatt tatgtaagac tctctgccgg
    32641 gctccatttt catcatgccc agcacattct ccgcgtactc gtccacattc ttaaccgata
    32701 ctatggaatc tatagccatc tgaagcctga ccgcttcgct tttttcaaca gccagcttct
    32761 gctctaattt tgttatctcc ttagtggttt cgtcaagtac aacattgcag taaattttta
    32821 ccgacagcat tgaaaacaac aacgccataa ccgcaaatac ctttacgctc ttttgcatat
    32881 tggcgcgcat ttgcagcttt attgcttttt ttgtgtcggt ctttttctgc accttggggt
    32941 agaccttgag gtgcgggtgc tcctttgtct ttttctgagg agctcttttg ggggctgcgc
    33001 tgcttgttac ctttttctcg tatggctcaa acaatgaaaa atcatatgct tctgttccat
    33061 agtatgaagg cattgtcagt ccctctttct taagattttc tattctttag gcagttttct
    33121 tactgctctg agtcttgcgc ttctgcttct gttgttttca gcgatttccg cctcgctggg
    33181 ctcaatcgat ttaaatggca gttccccttg cggaaccttg ccgcaaacgc agacggggaa
    33241 atcctttggg cactcacagc ccttgcagta tgaagcgaat atctgcttta ctattctgtc
    33301 ctcaagggag tggaaggtta taaccgccaa aattccgtta ccattgagga cgctgaacat
    33361 attgtctatt gcctcgggga gcagctcaag ctccctgttt acttcgattc tgagcgcctg
    33421 gaaggttctt cttgccgggt gaccgccctc tctgcgggct cttgcgggaa ttgagtactt
    33481 tataatttcc gcaagctcgg aggtggtttc gatgggcttt tttgctcttt cttttacgat
    33541 gttcgcggct atgctctttg cgaacttttc ctcgccgtat ctgaagatta tgtctcttag
    33601 ctgactttcg gaatatccgt tcaccacatc tcttgcggaa agccccgact tgtccattct
    33661 catgtcaagg ggcgcgtctt tatgaaatga aaagcctctt tcgtcgttgt caagctggta
    33721 tgagctcacg ccgaggtccg cgagaattcc gtccacgccg tcgatattaa gctctgagag
    33781 aacttgtttt atatccgaaa agtttttgtt gactatcgtg accctgtcat cgcccttaaa
    33841 gcgttcggtc aagacggaaa cagcgtcggg gtcgcggtca atggcgatta gtctgccgtt
    33901 tttaagcctt ctcagtattt cggcgctgtg accgccgccg cctgccgtgg cgtcaacata
    33961 gataccgttt tctttgattt caagggcgtc aaccgattcg gcaagcaaaa ccgatttgtg
    34021 aacgaattcc atttgactcc tcgctttgct ttagtaaata atctcgggat aaatatcgtc
    34081 ggtcatttcc tcagaggatg ccatagcctc gttgttccat ctgtctaaat cccaaatctc
    34141 gattcggtcg gtcatgccaa atatcattac atctttttta aggcccgcgt ggtcaacaag
    34201 gaattggctt aatgttattc tgccctgctt gtccggctca acggtgagaa cgccgtagaa
    34261 cgcgcgcctt tgcctttttc tgtcctctat ggtggcggac tgccctttaa tctgatttgc
    34321 tatgctttcc catttttcgt tggtaaatat aaagaggcag ccgtcggggg cgggatatac
    34381 gatgaaggag tcgccgagtg cttcacggaa acgggcgggg atgaatatgc ggttcttaga
    34441 atctattgtg tgcttaaaat cgccctgaaa aagcattctc gaatcctcct ttcatcaaaa
    34501 tttcgggtga gtgatgattt tatgccccta ttttagtaat ttttaaactt tttgctccac
    34561 ttctcaccac ttgtctaaca ttatatattg caaaaccatt aattgcaagg cttttaggca
    34621 aactaaataa aaaaaattgt cgcaaaattg tgcctgaacg agcaataatc cacaatatat
    34681 atgaccattt tttgatttaa gccggcaggg aagaattttt gaccgaattg tctaaaaaat
    34741 ttaaaaaatt ttgtgagtga gcgctaaaaa aataagaatt gatctgatga gtttgaacaa
    34801 aaagtgttga tttatcggat tgcctgtgtt attatttggg tagcagaatt tggctaaaga
    34861 tccggaagag aggtgaattt gatggatcaa cttatggctt tcctcgagaa gctgattcag
    34921 ttcctcatcg atattcttta cagcctgggc attgagctgg actatgacaa gctcttcccc
    34981 accgaaccca cctctgagga ataattttgg gcacccaaag caaaagagga tacgccgtat
    35041 cctctttttt tgctctgatt tttgctatta tcctgtattt ttgcgcttaa agctcaaaaa
    35101 aactgtttgc aaatagaatt aaagctattg atttacgctt tagaatgtga tattatgtct
    35161 ttcgcgcggt ttgcattgca ccagctttcg ctgtgcgcat ataatttaat acacacttcg
    35221 gggtgtggcg aagtttggta tcgcgcttgg ttcgggacca agaggccccg ggttcaaatc
    35281 ccggcactcc gaccagaaaa cccgt
//
LOCUS       DAONVQ010000004        32981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_856_length_32981_cov_5.075199, whole genome shotgun sequence.
ACCESSION   DAONVQ010000004 DAONVQ010000000
VERSION     DAONVQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 32981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32981
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_856_length_32981_cov_5.075199"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(406..624)
                     /locus_tag="PLD98_00705"
     CDS             complement(406..624)
                     /locus_tag="PLD98_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQA04809.1"
                     /translation="MIDRIKELICEFVKVNPKDLNEETNLRSDLGLSSLDLVNLAVAI
                     ENEYGTVISDREIAGIATIGDIIAYLNK"
     gene            complement(643..2385)
                     /locus_tag="PLD98_00710"
     CDS             complement(643..2385)
                     /locus_tag="PLD98_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQA04810.1"
                     /translation="MTKEFEIWKEQNPKLFAQPPLKDMKQLVKRAAEMHGERAAVKER
                     KGGEAISHSANELKESIEAIGTALIDMGLEGKNIAVLGENSYHWILAFLSIIGGVGVA
                     IPLDKELTDKDLARLMAKGEIEAVFSSRTYIGAVKQHKEESDKLRYQFLMSSDSPAEG
                     FITVDELIARGKELISKGDRRYIDKAVSPDDLAAVFFTSGTTGANKGVMLTHKNLVTN
                     IEGMVHNVVFRDSTFSVLPMNHIYELNCNILPLIYIGVTICINDSLKNLMSNFQLFKP
                     HMVMLVPTFVENFYNAIWAEAEKTGQAEKLRQAVEYSNSLLEKGIDKRAEIFGFIRQK
                     FGGNLDLMICGGAPCNPKFIKGLADFGFRFYLGYGLTEAAPIASLNLDGGKYPASTGT
                     PFHKTEYILHDTDADGIGEVWLRGDNISCGYYKDSKATSESFMDGWFKTGDLGKIDEN
                     GHLVLVGRKKSLIVLDNGKNVHPEEIEDLVKDEIPYIREIVVLEAKKEILGSNQSIIA
                     AIVFIDMADFPDKSMEEIWQMASSDINKVNKKLPGYKMIRDVTVVTEDFEKTSTKKII
                     RQKAIERYRLVSSR"
     gene            complement(2404..3789)
                     /locus_tag="PLD98_00715"
     CDS             complement(2404..3789)
                     /locus_tag="PLD98_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HQA04811.1"
                     /translation="MKNKMAGTAYDLIPSQQTMHYMLKYSLHKQVMQIPMSLTVSEKL
                     DFDTLQKAVEIECRRNDCMRLRYFKEKGQIRQYFTPDCKIGKIPVVTFKTKEEQEKHL
                     TADAQKPIRFLKGEVFRIIFFRSYNGGTGIYINVCHLSMDATAVFLFFTDVLKVYNAL
                     KDGTEMPEPLYSFKDIIDKEFKYLADKERYKKDEEFYREYFLKDGEPFYAGVHGMDLL
                     LKERKKKKNPNIRVPSAFDPIHDKADLIKITLDKKDAKKIFDFCGKHNISPACIIQMG
                     LRSHVSKINERNPDVYFMELCNRRVSYKDKQTGGCVTQPLPVRAVIPEEKTFMEALEQ
                     LSGIRLQVYRHMNYPYMDSRNLVRELFGYGMTASPSSMMFTWLPLEFDCGKNWSYEFS
                     GYNLGRYIMPLYTFSMPNLKNGGIDFYYMHRTNTISAEQIRALHTGAVKAILKGIENP
                     SITVGQLLNEI"
     gene            4183..4956
                     /locus_tag="PLD98_00720"
     CDS             4183..4956
                     /locus_tag="PLD98_00720"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HQA04812.1"
                     /translation="MILYFTGTGNSKFAADFLAEYLGDECLSLNEKIKKGEDIKENSE
                     KPYIVVAPIHAWRFPLKVEEIVKRAELKGSRKIYFVGTMGENSGKTDTCLKKLCGEKG
                     LEFMGFCGVKMQNNYLIGDVMPNKAVAEENLHEVIPALESIAEKIKNGEKIEKTDKTP
                     FTFLVSGVVNKFFTKFAGKYMKPTASDKCGACGICAEFCPVNNIEIKDGKAQMGEKCI
                     SCFGCIHRCPTEAVNIEGKTEKNGRYLCPEYSQWKKAHE"
     gene            complement(5019..5861)
                     /locus_tag="PLD98_00725"
     CDS             complement(5019..5861)
                     /locus_tag="PLD98_00725"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HQA04813.1"
                     /translation="MREIDARAITKAVRDACIEANLKLPACLQRRIEEAYQTEEKQLP
                     KGVMGDLAENLKAAEELSVPICQDTGMAVIFAEVGQDVRIVGGDFETAINEGVRQGYI
                     EGFLRKSIVKDPLRRVNTEDNTPAVIHTRIVAGDKLKLTVSPKGFGSENMSALKMMLP
                     TSTKEDIMEFVIDTVKKAGSNPCPPIVVGVGIGGDFEYSAILSKKALCRSVDIRHPDE
                     FYREMEEELLKRINELNIGPQGFGGKTTALAVNIETAPTHIAGLPVAVNIGCHVTRHA
                     EVVL"
     gene            complement(5890..7035)
                     /locus_tag="PLD98_00730"
     CDS             complement(5890..7035)
                     /locus_tag="PLD98_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQA04814.1"
                     /translation="MLFINAVIHTMAGSVIENGFIETEGSAIKRVGSVSELSESEKSD
                     AIDLRGDSVYPGFIDTHTHLGLVGDALGFESDDINEMTNPLTPHLRAIDAINPLDLSF
                     KEAAQAGVTAVVACPGSSNPIGGQTAAIKTHGVCVDDMIIKAPLSIKMALGENPKTVY
                     HERKESPSSRMATAAAIRGILNEAKEYLAKKEKDEDQDYNQALEALIPLLKGEIPAYI
                     HAHREDDILTAARIAREFGFDYIIVHGTRGHLVAERLIKENIKIMSGPLLCDRSKPEL
                     KDLTPAAPAILKKAGIEISIVTDHPVIPIQYLPLCAGLAAREGLAHEDALRAITIDAA
                     KMAGIENRVGSIEVGKDADFSVFSEDPLTISAKPKMVVINGEIVHKA"
     gene            complement(7330..7770)
                     /locus_tag="PLD98_00735"
     CDS             complement(7330..7770)
                     /locus_tag="PLD98_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQA04815.1"
                     /translation="MQLQVGDIVEGKVTGITNFGAFVKLAGGETGMVHISEVSTSYIK
                     DIKEHLSENQEVRVKILAFGEGGKISLSIKQALPPEERNRGHQHQGFRNNAERSADSG
                     EQQSENQSFEQMMAKFKQASEEKMSALKRYTESKRGGGYSRRGR"
     gene            complement(7804..8097)
                     /locus_tag="PLD98_00740"
     CDS             complement(7804..8097)
                     /locus_tag="PLD98_00740"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQA04816.1"
                     /translation="MKKNGIRKHSLILLFVGLIGVCYFVFSMIRLSSDIKAKQNEAES
                     LSQAYQQQQIENEEKRRILADGNEKELIEKYARENRGYVYPDERVYYDITPGG"
     gene            complement(8069..8785)
                     /locus_tag="PLD98_00745"
     CDS             complement(8069..8785)
                     /locus_tag="PLD98_00745"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HQA04817.1"
                     /translation="MYGIELAHQTRLFFMAVGIGFFAGAVFDIFRILRLAVNFGKIAV
                     AIQDVIYILFVFALSFAFLLSKNMGEIRMFAVLGELLGFIIYYLTFGSFVYSIGDRII
                     EFLRRLFIKVFRIISYPFIKLFGLIRKLCEKIGAALRKTAIKTKNKLILRLQRIKALL
                     YNVFGFYFSRRKAAAAAEGPPKPLSLEERRARIAQEMEMFMRGELPQMSPPAKQNSGP
                     RKKGSKKGSKDYEKKRHKKA"
     gene            complement(8785..9063)
                     /gene="yabP"
                     /locus_tag="PLD98_00750"
     CDS             complement(8785..9063)
                     /gene="yabP"
                     /locus_tag="PLD98_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HQA04818.1"
                     /translation="MAEEKRTGMPHNLVLENRRKLSISGVLDVDSFDESTIIVNTEMG
                     ELTIQGQDLHINNLSIETGEMCIEGSISTLLYSEIEKRSGGFFSKVFR"
     gene            9270..10271
                     /locus_tag="PLD98_00755"
     CDS             9270..10271
                     /locus_tag="PLD98_00755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQA04819.1"
                     /translation="MVKVSVVVPVYNVEKYLAKCLQSLIGQTLKDIEIIVVNDGSADG
                     SQKIADEFKERYPEIIKCVVKENGGLGSARNAGLERASGEYIAFIDSDDYVKADMLER
                     LYDSAKKHDSDVAIGGNIYVDENGDVLREEIFSYGKECFNADETRDVLLSSIGVCNKI
                     YRADFLRKIGAKFRVQKWYEDFDFIIKVLLSAQRISVVEDAFYYYLQRQGSIMNNSNL
                     IRNLEILEALSETLKFCKERNLLEKYYDEIEFLALLHAYSAASVRIINADADKKDKLL
                     ALEEIKSYMDKEFKDFKNNKYIGSHLSRNKKIVMRLLDLKLYTLIGLMFKAKRVLAR"
     gene            10289..11818
                     /locus_tag="PLD98_00760"
     CDS             10289..11818
                     /locus_tag="PLD98_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQA04820.1"
                     /translation="MQLIKDRKRLVTGFITVFVLLQPFIDVFTSLAIRNSMGASLGVL
                     VRSFFMAVITAYCILLSKTDKKLKLGVALYMIATGGYLMFYFAHFFKTGGLFFAFTNI
                     KEAVKVFYFVFLLLGFYLIYKEYKFTVSDTVLSITAALYMSIIVIAFVTGTSYPSYKH
                     GEGFVGWFYSANEIGAIIGILAPYLLVNSVKGLIKALKEKKYPLFALYSFLCLETVFC
                     ALFIGTKAVFLAFMLFVAIALALALFKAYKKAKTVNISRKAVSSALIAAVLCVSVFSG
                     LYIVSPIRANLTGKAVVEYTYLQKGTPFDPVGEDPVELPGGFEPGEGNEQKDAELYTV
                     WDAEKEPLPVPVSVLNKVFNNRIVFSIPVSRHYMSSSWVEKLFGVGYVNRSDAVYDIE
                     EAIEIDIVTIFLRHGVVGFVLYFVPAFTLAFWAISRIFSSKRDIKKRDCVLVSLFGIA
                     VAFGMSVLAGHVLIAPAVSVYIAIAFIKLCANAENAEEHDMTEIVDKSLSSCQKEPQT
                     L"
     gene            12010..14040
                     /gene="recJ"
                     /locus_tag="PLD98_00765"
     CDS             12010..14040
                     /gene="recJ"
                     /locus_tag="PLD98_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQA04821.1"
                     /translation="MPRRKWITAETDKEKAALLAAETGADSLAALILLSRGYDTPQKI
                     RDFLSGTGLSSPFCLKDMDKAVERILRAVDNFERITVYGDYDADGVTATAILYSYLDL
                     MGANVDYVIPSRESDGYGLNFELIDMIKQRGTNLIITVDNGINSVKEAKYIKELGMDL
                     IITDHHIQSGELPDAAAVVNPHRTDETGQLSMLSGAGVAFKLVCALEGEDTENMLQDY
                     ADLLAIGTIADIVPLCGENRALIAAGLRCINEAPRPAIEAIRAASGFGGRYFSSRAVA
                     FTIAPRINAAGRMGSAESALKLLLSEDYDEAFRLAEVLEKANKDRLEAEKRIYDEIER
                     IFDKDPSLYQDRVIVVSGSGWHPGVIGIVAARLCERYGKPAVVISVDEDNIGRASARS
                     IEGFSIFEALKATGDLLSHYGGHTLAAGFTIEEGKISEFRKAVNEYAGTVKRAVPTLF
                     IDCRLNPKYISNSVLESLEFLEPYGAGNPTPVFGLFGMEIQSVRPVGNGHIRLLLSRD
                     GTRIDAVKFGVSVDEFPYAVGDRVDLAVVIEKNEYMGTIRPSIQIKDIRFAGTDDEAL
                     FENLIKYEEISSREASVKEKCSPYCPTREFMGNLFRFLRANGGWRHDLEILSYRMGLE
                     PSEIVKLGLALDALCELQLIYRLKTGGYAMAENPKKVQLCSSKILTNLGYTE"
     gene            14070..16253
                     /locus_tag="PLD98_00770"
     CDS             14070..16253
                     /locus_tag="PLD98_00770"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQA04822.1"
                     /translation="MTEISMEEKRAYESFISKVRENYSKEDADLIDLAYRKASAAHIG
                     QTRESGEPYIIHPIAVAEILMGLGMDAQSLIAALLHDVVEDTDVCIDEIGCGFDSDVA
                     ALVDGVTKLNKVPLASKEEAQAENIRKMLLAMSEDIRVIIIKLADRLHNMRTLSFVSE
                     QKRRDKALETLEIYAPIAHRLGIRPIKEELEDIAISYLDPIAYNDIKESLEAQSKSRN
                     EFLEKIKARIGERISEIVKGAKIEGRIKSVHGIYRKMYMQNRTFDEIYDIYAVRIIVN
                     TVIDCYNCLGVIHDMFRPIPGRFKDYISTPKPNMYQSLHTTVIGKEGIPFEVQIRTWE
                     MHRTAEYGIAAHWKYKLGLKGKQQFEERLAWIRQLLELQKDSEDVEDIVRTIKTDLVP
                     EEVYVLTPKGDVISLPQGATVIDFAYAIHSAVGNRMIGAKVDGRIVPITHVVKTGEII
                     EILTSNQTKGPSRDWLKIVKTSEARSKIRQWFKKQNREENIEVGKSELEREFRRSNIR
                     LEEEGMNELIQKIAERYNFQDIQDFYAAIGYGGVSISRILPQIKEEYNKLIKAQSPTP
                     VKLKMKSAASSEGVIVEGIENCLVKLSKCCNPIPGDDIIGFITRGHGVSVHKRDCSNV
                     PYPIESAPEPDRWIPVRWDSDIKEDFNAALLITCLASVSIMADISGALANMHVMIHSL
                     NTRETKDGRYLVTVTVTVKSLEHLRSVMSRLDKINGVFNVERSGL"
     gene            16281..16457
                     /locus_tag="PLD98_00775"
                     /pseudo
     CDS             16281..16457
                     /locus_tag="PLD98_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199305.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
     gene            16623..17081
                     /gene="dtd"
                     /locus_tag="PLD98_00780"
     CDS             16623..17081
                     /gene="dtd"
                     /locus_tag="PLD98_00780"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQA04823.1"
                     /translation="MKAVIQRVENAAVTVDGRVVGSIGGGYMILLGVVDTDTEAEARL
                     LAAKTASLRVFRDENDKMNLSILQVGGEALVISQFTLCADTRKGNRPSFTSSAEPKRA
                     NELYELYMDELRKNGVKKVEKGEFGAHMKVSLVNDGPVTIILDTDTWKRA"
     gene            17095..17742
                     /locus_tag="PLD98_00785"
     CDS             17095..17742
                     /locus_tag="PLD98_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQA04824.1"
                     /translation="MEIISIKPFGNFANTYIIKDESGAAAIVDPGCVTDELKSEIEKC
                     GKVEYILLTHGHYDHIWSAKEIKELTGAKLAVHKNDMPFLADESLSHGCKYFPGEQDV
                     PQPDIVLEGGEVLKLGNSEIKVMSTPGHTKGSVVYIVGDTMCAGDTLFYLTVGRTDLE
                     GGSMEDMINTLEKLKNLEGDFRVLPGHGRETTLEHERQNNRYMSKLDLLKEQYLG"
     gene            17752..19266
                     /gene="hemZ"
                     /locus_tag="PLD98_00790"
     CDS             17752..19266
                     /gene="hemZ"
                     /locus_tag="PLD98_00790"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HQA04825.1"
                     /translation="MKLYLNHPFHYEAENLTRLFFPSEKIQVIREPFIEGESKAPSEN
                     SDGSEGFIVSSLQKADGSYKITAYVKVGERESYDESFVSDTLAEKGFFTEEKQGEERD
                     KAELLMAQCLYKALSELTSYTPPWGILTGVRPSKLYRSLYEEKGYGAFDFFKNELFVS
                     ENKIKLLHSIVTRQKGIIDLSKENSYSLYVSIPFCPTRCEYCSFVSHSISHFKKIPEY
                     IGLLIKELEEISKIASELGLKLETIYFGGGTPTSLNEAQLKSVTDAVAALFPLEGVRE
                     YTIEAGRPDTLNEEKLRIMKAAGVTRISINPQTFKDEILRKIGRCHTAAETIDAFNMA
                     RQMGFDNINMDLITNLPGDTPEGFMFSLNKTMELNPESVTVHSLAVKRSSALARTEGI
                     NAEWEPSVYENAYKALEEKGYNPYYLYRQSKSAGNRENTGFSKEGFESLYNIYMMEET
                     HTVLAAGAGGVSRLKEPNGEFIDRIFNFKYPYEYVSNFEEILRRKDKIKAFYEF"
     gene            19328..20266
                     /locus_tag="PLD98_00795"
     CDS             19328..20266
                     /locus_tag="PLD98_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HQA04826.1"
                     /translation="MQNLCNSLSIINNNIRDNPDAFIFESEKFYHDQLKKVIELIKDR
                     SGREIIMLAGPSSSGKTTTADKLLSALTNGGRQGYVISLDDFYLNAGEGPRKEDGSFD
                     FESVHALDLPLIHSSIEQLLSGKETKLPLFDFTVGKRSDKTRSVTLREHDFVIIEGLH
                     ALNPLITSSFSSANLIKLYISVNSDIYDGDDVLLSRIELRFLRRLVRDYKYRNSSADN
                     TFMLWTGVVEGETKYLLPHRENADIRLNSFHPYEVCVLKDMAAPLLQSAVKEEYKKEA
                     VRLLKSISKFDTCPCGKVPADSLLREFIGPMEECKF"
     gene            20312..22087
                     /locus_tag="PLD98_00800"
     CDS             20312..22087
                     /locus_tag="PLD98_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HQA04827.1"
                     /translation="MAESGMKRQSLLNGALVLVIATALVKVIGALFKIPITNLIGPVG
                     RGYFGSAYNIFIPVYNIAMAGLPVAISKLVAQNIALGRYRDVRLIFKIAKRLFFLTGV
                     VGMLILMILAYPYAKSINTTQVIPSILVIAPSIFFCCMMSAYRGYYSGLRNMNPTAVS
                     QVIEALGKLILGTLLAKFTMDHGMNNFKEGKKVFGKVVTTEAEALSATYPYAAAAAVA
                     GVTIGTVFALGYLILRHRLKKDGISKAMLRSSPKPLQVKNLTYQLIGIAIPVVASTII
                     MNLTNFIDSWSIQFRLSHAIASNEGLIRAMYETALNTANIVTSDLKTYLYGAYETALD
                     FKNLIPAFTMTLGVSAIPVLSEAWTLKNKREIKKAVESVIRVIMLIAMPAGIGMAVLA
                     KPILSILYKDMSSLSIAAPVMAYYGYATFLIAFSQPLTNMLQGIGRMDIPMKTLAVAA
                     VFKILINFIFVGIPKYNIFGAVIGTVVFYVIIVAGGMFFLIRETKVKLNYFNIFIKPL
                     FCSVLCGAAAWSSHGLLSMVLKFGDPSSRLNGANVATLLAIGIAVVVYGLSLLFSRTI
                     AKTDLNMLPGGEKISKILEKYGFIG"
     gene            22179..22895
                     /gene="mazG"
                     /locus_tag="PLD98_00805"
     CDS             22179..22895
                     /gene="mazG"
                     /locus_tag="PLD98_00805"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQA04828.1"
                     /translation="MKRLNFEFKDKYNINDLLRIMEILRSPEGCPWDREQNHRSIKKN
                     LIEETYEVVEAINKDDKELLREELGDLLLQIVFHSQMEKEQGVFDFDSVCDGICKKLI
                     ERHPHVFGTLEVKDSKEVLENWEQIKRRTKKRDTVADALEAIPKELPALMRSDKIVSK
                     AEGSYFEKKGVKEAAKALCDFARDIENEDKPAESAEFVGKLLFEAVRLSRALKVDPED
                     ALARYCTSLVEELKAAKPEK"
     gene            22989..23264
                     /locus_tag="PLD98_00810"
     CDS             22989..23264
                     /locus_tag="PLD98_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQA04829.1"
                     /translation="MNKSDLINAVASKAELTKKDAEAAVSAVISSISEALASGESVQL
                     VGFGTFEVRDRAAKQGRNPKTGEPITVPAAKVPAFKAGKALKDSVNK"
     gene            23446..23688
                     /locus_tag="PLD98_00815"
     CDS             23446..23688
                     /locus_tag="PLD98_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HQA04830.1"
                     /translation="MRLDKYLKVSRLVKRRTVANELCDAQHVSVNGKVARASYDVKVG
                     DMIEIRTGNNLIKAKVLQVTEHATKENASQMYELIK"
     gene            23722..24975
                     /locus_tag="PLD98_00820"
     CDS             23722..24975
                     /locus_tag="PLD98_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQA04831.1"
                     /translation="MRTDKGQIKIVALILALCLVCSGVGGAAGALTAYRIFTNKLQAD
                     LPKEPDEGEALTVPPALTTEAEVTTEPVTEPRTEKQTEPQTERQTNPAPISLSAKEIF
                     NKTVSSVVAIKTVVTFKSSGILGTVTREGTGSGSGFIYTQNGYIVTNYHVIQSAKSIK
                     VTLHDNKEFDAKLIGYDQENDIAVIKIDAENLNAVTVGSSSELSVGDRLYIIGNPLGE
                     LSQTLSQGVVSGLNRKIDTGNYIINMHQTDAAINQGNSGGPVFDENGRVVGIVAAKYA
                     SETIESLGFFIPIDDVKTKLNDIMKYGYVRNKPYLGLSVQTVTNLLSLQYRIPVGVYV
                     VDLDTAGPAAKAGVQAKDVITKVGGKAVTSVAELKNELSKFKTGDKVELTVSRSGGGG
                     VFTVTLGELVPTTKPRTNYSDVYDV"
     gene            25158..25964
                     /locus_tag="PLD98_00825"
     CDS             25158..25964
                     /locus_tag="PLD98_00825"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQA04832.1"
                     /translation="MSFDSSFLNTEKSDGGRAWRIEALGGGVSAVVSREHNFGTDALL
                     LAYFAEPKNIDRVCDLGTGCGIIPLYWRSRGVTAYIAAVEIQESAYAQVCEANRINPP
                     DKSVVPVLADLRNLKGILPFGEFDLVTMNPPYFKGGIMSEDKERMTARHDLECTLDDV
                     CRAAASLLRFGGRFCMCLPPERLCDAVELMRKYSLEPKRLRFAKYKEGDKPYLMLIEG
                     RRGGKPSLAVLPDIVLTENGKGESKEMKEVLSEYLKMKACNTSGRKENER"
     gene            25954..26787
                     /gene="rsmI"
                     /locus_tag="PLD98_00830"
     CDS             25954..26787
                     /gene="rsmI"
                     /locus_tag="PLD98_00830"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQA04833.1"
                     /translation="MSGKLYIVGTPIGNLGDISERALATLKECDFIAAEDTRVTLKLL
                     NHFDIKKPLVSYHEHNKRERGAMVADRILKGETCALVSDAGMPCISDPGTELVNTCIE
                     RGIPVLAVPGPSAVITALAVSGLNVSRFTFEGFLSVNKSSRKKHLEEIKGEKRTMVFY
                     EAPHKLPRTLKDLYETLGDRRIALVKELTKIYEQVERTTLAAAAEKYSQQKPKGEFVL
                     IIEGREELKSETEPLSAEDAAELARQYIEEGLSPTEAAKKAAKESGHKKNDIYRLLTS
                     D"
     gene            26805..27467
                     /locus_tag="PLD98_00835"
     CDS             26805..27467
                     /locus_tag="PLD98_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04834.1"
                     /translation="MKGVKTLGGEIFNIAISGLSERQILYLLWTAAAALSVVTVFVII
                     KAGKAYKRRQEIVGEQMAQMERDRALYEKYKNADFETLKNAEPRELIYGLALQVQAQV
                     QNAPNPNELFMSLPSFQRYIYSLDIFLEDTEKSLSSFFRNNGEPLLSSAIDALRETGL
                     LTTEIERLCLMFDKDSEVSFDEEYIKKADESFGDSFNRETALKKIKEYILRENSESDK
                     NC"
     gene            27570..29333
                     /locus_tag="PLD98_00840"
     CDS             27570..29333
                     /locus_tag="PLD98_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA04835.1"
                     /translation="MRRFLKLLRPYAGLLALSLLMVIVSNAMQLVLPQLTADLVNEGI
                     MKGDLETIKYKGMLMLAVSALGLVISVVNSYCSSKTSSGYAMVLRREVFHKVESLSQS
                     DVDKIGVPSLITRTTNDIRGIQDFILSGLRILVTVPILLVGGLVMSIKMNPELTKILL
                     IIIPIIGVIAFILYLFLSPKFALMQKKTDQLNKIVREKISGIRVIRAFNRTDYEDERF
                     KTANLELTDISLTINRFMAGLLPVGLFIIYGLMAILLWVSTTNIAKMDAVTQADRISS
                     TIGNLQAFIVYIGLVVFAISMAATIFIAIPKARISLKRINEVLNMEPVVVEKENPAFI
                     APEHRKGVLEFRNVSYSYPGSDVPAVKNVSFKCSPGEVTAVIGATGSGKTTLINLIPR
                     LYDVTEGQILLDGVDIRELSFDELHSRLAFIPQKAFLFSGTVEDNLRFGKEDATDEEI
                     MRALEIAQAKTFVEALPEKTQNLISQSGTNLSGGQKQRLAIARAIIRGADICIFDDSF
                     SALDLTTDARLRAAIKQHLSGANIIIVAQRVSTILDADRIIVMDKGEVVGIGTHAELL
                     ESCEIYRDIVRSQHLEEEVSE"
     gene            29330..31234
                     /locus_tag="PLD98_00845"
     CDS             29330..31234
                     /locus_tag="PLD98_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA04836.1"
                     /translation="MSEIKAEERRLQKSEKKVKVSREKLSHRYSGYHRKRINQQKPQN
                     AKGTFRRFVGLIAKYKWQMVLIAIATVIIALLNVWGPDILGEVIDAIKRQIDLKLQTN
                     TNDFTEIKQLIFKIMAIYMGSAVLTFIQQYTLAGVSQQLVCSLRERINRKLSKLPLKF
                     FDTNTKGDILSRITNDIDNVSNSLQSNMVTIITNVVQLITVFAIMLYTNWLMTAIIVV
                     FVPLSTLISYRISRISKRLFRDLWDRTGELNGHIEEMYTGHSIVKLFGYEKQSIAEFD
                     QINEDLMKTASKANFISGTINPLLTFVNNIVYVLLCFVGGYLVLNNRITLGDIAVFFA
                     YSRMFSGPINNISQVINSVQSALASAERVFNVFDEEEEPADSSKALIKEPKGYVEFEN
                     VSFSYVKEKPLIENMNLKVNPGELIAIVGPTGSGKTTFVNLLMRFYDVDSGSIKIDGV
                     DIRDIPRENLRSIFGMVLQDTWLFKGTFRENITYGKMNATDEEVIAAAKSARVHDFIM
                     SYPDGYDTMLDEGGANISQGQQQLITIARAILADPAVLILDEATSSVDTRTEMHIQEA
                     MRTLMKGRTSFVIAHRLSTIRNADKILVMRRGSIVEQGTHEELMAANGFYATLYNSQY
                     VGGIPPEESE"
     gene            complement(31570..32217)
                     /locus_tag="PLD98_00850"
     CDS             complement(31570..32217)
                     /locus_tag="PLD98_00850"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HQA04837.1"
                     /translation="MEKSKVLQRISDKGIVAVVRAESEEQALKIAEACIKGGIDAIEI
                     TFTVPRAEKVIERLCKEYDAETLLVGAGTVLDAETARVAMMAGAAFVVSPGFDEGVVK
                     LCNRYRVPVMAGVMTVTEAMQAMSAGADVLKVFPAELFGPAIIKAFKGPLPHGVFMPT
                     GGVDTENVAQWIKAGAFAVGVGGSLTAGAKKGDYEDITNKAKILIERIAEARVSK"
     gene            complement(32237..>32981)
                     /locus_tag="PLD98_00855"
     CDS             complement(32237..>32981)
                     /locus_tag="PLD98_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQA04838.1"
                     /translation="VGVSVRGNTAASQLKPGDIDWDYIFGTLGVRWMHTGGIFAALSE
                     TTAQVVIEAVKAAKKYGTIVSYDLNYRPSLWKSIGGKEKAQEVNKEIAKYIDVMIGNE
                     EDFTACLGFEIEGNDENLKELNLEGYIKMLGKAVETYPNFKVIGNTLRTVKTATINDW
                     SAIVYADGKVYRGMEFKNLEIFDRVGGGDSFASGLIYGLMTTGDPQKAVNYGVVHGAL
                     AMTTPGDTSMASLKEVEAIMAGAGARVVR"
BASE COUNT         8633 a         8026 c         8246 g         8076 t
ORIGIN      
        1 gcccccagat tgccgctgaa aaatgcggcg gtgcgcactc cccgccgccc cgcctccgct
       61 gagggtgaca gggtttttcc atgtgcggcg gcgccccctg cgtggggagt ccgctcggtc
      121 ggggcggaac acgctcggcg caaaggaagc ggagagtcca ccccacgctg cggtgtgtgt
      181 ctgacccgtg tgcgtcaatc ggagatcccg ccgcctgccc ccgcccgccg ccggttgggc
      241 ggcgaccttt aaagtgagct gcttttctcg gcggcagacc tttaaaggca ataaaagctt
      301 aaaaaccgcc ctgtcggacg gtttttaagc ggggtgtagt gcttgagtga gtgtgaaaga
      361 atggcgattt cgctattggt gttatggaaa cttctgcttg ccttcttatt tgttaaggta
      421 tgcgataata tccccgattg tggcgatgcc tgcgatttcc ctgtcggaaa ttacggtgcc
      481 gtactcgttt tcgatggcga ccgcaaggtt tacaaggtca agggagctga gtcccaaatc
      541 gctgcgaaga ttggtttcct cgtttaaatc cttggggtta acttttacaa attcgcaaat
      601 taattcttta attctgtcaa tcatattatc ctctcctgcg tcttaccttg agcttacaag
      661 cctgtatctt tcaattgcct tttgcctgat tatcttcttt gtggaggtct tttcaaagtc
      721 ctcggtcaca acggtgacat cccgaatcat cttatagccg ggcagctttt tgtttacctt
      781 gtttatatcc gatgaagcca tctgccagat ttcctccatt gacttatcgg ggaagtccgc
      841 catgtctata aagactatcg ccgctattat gctctgattg gagcccaaaa tttccttttt
      901 ggcctcaaga accacgattt cacggatata ggggatttcg tcctttacaa ggtcctcgat
      961 ttcctcgggg tgaacattct tgccgttgtc aaggactata aggctcttct ttctgccgac
     1021 aagcacgaga tgaccgttct catctatctt gcccaaatct ccggtcttga accagccgtc
     1081 cataaaggac tcggaggttg ccttgctgtc cttgtagtat ccgcagctta tgttatctcc
     1141 tctgagccag acctcgccga tgccgtccgc gtcggtgtcg tggaggatgt actcggtttt
     1201 atggaagggc gttcccgttg acgcgggata tttgccgccg tcgagattga gcgacgcgat
     1261 gggagccgcc tcggtaagac cgtagccgag atagaacctg aatccgaaat cggcaagacc
     1321 ctttatgaat ttgggattgc agggagctcc gccgcaaatc ataaggtcaa gattgccgcc
     1381 gaatttctgc ctgataaatc cgaaaatctc cgcgcgcttg tctatgcctt tttctaaaag
     1441 gctgttgctg tactccactg cctgccttaa tttctcagcc tgacccgtct tttcggcctc
     1501 agcccaaatg gcgttataga agttctctac aaaggtggga acaagcatga ccatgtgggg
     1561 tttaaataac tggaaattgc tcataaggtt tttaagactg tcgttaatgc agatagtaac
     1621 gccgatgtaa ataaggggca gtatgttgca gttaagctcg taaatatggt tcatagggag
     1681 cactgagaat gtggaatccc tgaacacaac attgtgaacc atgccctcga tattggtaac
     1741 aaggtttttg tgggtcagca taacgccctt gttggctccc gttgtgccgg aggtaaagaa
     1801 taccgcagcc aaatcgtcgg gagaaaccgc tttgtctatg tagcgtctgt cgcccttact
     1861 tatcagctcc ttgccccttg ctatgagctc atctacggta ataaaacctt ccgcggggct
     1921 gtcggaagac ataaggaact gatagcggag tttatcgctt tcctccttat gctgctttac
     1981 ggcgccgatg tatgttctgc tggagaatac cgcctcaatc tcaccctttg ccataaggcg
     2041 ggcaaggtcc ttgtcggtaa gctccttgtc aagagggatg gcgacgccca cgccgccgat
     2101 tatggagagg aacgccagaa tccagtggta tgagttctcg cccaaaacgg ctatgttctt
     2161 gccctcaagt cccatgtcta taagggcggt gccgattgcc tctatgcttt ctttcagctc
     2221 gttcgcgctg tggcttattg cttcaccgcc ttttctctct tttaccgccg ctctttcgcc
     2281 gtgcatttcg gcggctcttt ttacaagctg cttcatgtct ttaagaggcg gctgcgcgaa
     2341 aagctttggg ttttgctctt tccaaatttc aaattccttg gtcatctctt tccccccctg
     2401 ctcttatatc tcgttcagaa gctgtcccac tgtgatggac gggttttcaa tgccctttaa
     2461 gattgccttt acggctcccg tgtgcaaagc tcttatctgc tcggcggaga tggtgtttgt
     2521 cctgtgcata tagtagaagt ctatgccgcc gtttttaaga ttgggcattg agaaggtgta
     2581 aaggggcatg atgtatcttc caaggttgta tccgctgaat tcatatgacc agttcttgcc
     2641 gcagtcaaac tcaaggggca gccatgtgaa catcattgag gaggggcttg ccgtcatgcc
     2701 gtagccgaaa agctctctga caagatttct tgagtccata tacggatagt tcatgtgcct
     2761 gtaaacctga agtcttatgc cggagagctg ctccagagcc tccataaagg tcttttcctc
     2821 cgggataacg gctcttacgg gaaggggctg ggttacgcag ccgcccgtct gcttgtcctt
     2881 gtagcttact cgcctgttgc acagctccat gaagtataca tcgggattgc gctcgttgat
     2941 ttttgaaaca tgagagcgaa gtcccatctg aattatgcag gcgggagaga tgttgtgctt
     3001 gccgcagaag tcgaaaatct tcttggcgtc ctttttgtca agggtaatct ttataaggtc
     3061 cgccttgtcg tgaatcgggt caaaggcgga gggaacgcgg atgttgggat tcttcttctt
     3121 ttttctctct ttcagaagca aatccatgcc gtgaactccg gcgtagaagg gctcgccgtc
     3181 tttaaggaag tactctctgt agaactcctc atccttcttg taacgctcct tgtcggcaag
     3241 gtatttgaac tccttgtcga tgatgtcctt aaagctgtaa agaggctcgg gcatctcggt
     3301 gccgtctttt aaagcgttgt aaactttcag cacatcggtg aagaaaagga atacggcggt
     3361 cgcgtccatt gaaaggtggc agacatttat gtaaattcct gtgccgccgt tataagagcg
     3421 gaagaagatt atgcggaaaa cctcgccttt aagaaatctg atgggctttt gagcgtcggc
     3481 ggtgaggtgc ttttcctgct cttccttggt tttgaaggtg actacgggaa ttttgcctat
     3541 tttgcagtcg ggcgtaaagt actgccgtat ttgtcccttt tccttgaagt agcgcagccg
     3601 catgcagtcg tttcgccggc actcgatttc aacggccttc tgaagggtgt cgaaatccag
     3661 cttttcgctg accgtaagcg acatgggtat ctgcataacc tgtttgtgca gactgtactt
     3721 gagcatgtag tgcatggttt gctgcgatgg aattaaatcg tacgcagtac ctgccatctt
     3781 gtttttcatt ttatttcccc ccaaagttaa ctaaagtcgg aaagtgaatc cgaattctgt
     3841 caaaggccgc attaaaatcc gaattttccg acctatgtgc tataataata taacgaaatt
     3901 attcataaat tgtgaacaaa ttgtaaactt cagggcgttt tttacaggga atttaagtaa
     3961 atttcaaaat ttttataaat atcgacaaag atttggggct ttccgttaat aagactttaa
     4021 agaattgtta aatatttgaa aataaggagg tgcattttga ctcaaaacag ccgctttcaa
     4081 agtgtcaaat taagtttttt gcggcttaaa gagaaaaatt gggaataaac ttgaaaaatt
     4141 tgttaacaag gttttatatt aaagagaaag gggagtgagc aaatgattct ttactttacc
     4201 ggaacgggca acagcaaatt cgccgccgat tttcttgcgg agtatctggg tgacgagtgt
     4261 ttaagcctta acgaaaaaat aaaaaagggc gaggatataa aggaaaactc cgaaaagccc
     4321 tacatagtcg tcgccccgat tcacgcatgg agattcccgc tgaaagtaga ggagattgta
     4381 aagcgggcag agcttaaagg cagccgcaag atatactttg tcggcaccat gggcgagaat
     4441 tcgggcaaaa ccgatacttg ccttaaaaag ctctgcggcg aaaagggttt agagttcatg
     4501 ggcttttgcg gcgttaagat gcagaacaat tatcttatcg gcgatgtaat gcccaataaa
     4561 gccgttgccg aggagaatct tcatgaagtt atccccgctc tggagagcat tgccgagaaa
     4621 attaaaaacg gcgagaaaat agaaaagacc gacaaaaccc cctttacctt tttggtttcg
     4681 ggcgtggtaa ataaattttt cacaaaattc gctggcaagt acatgaaacc cacggcaagc
     4741 gataaatgcg gcgcctgcgg aatttgtgcg gagttctgtc ccgtaaacaa cattgagatt
     4801 aaagacggca aagctcagat gggcgaaaag tgcataagct gctttggctg catacaccgc
     4861 tgcccgacgg aagctgtgaa catagaggga aagacagaga aaaacggcag atacctctgc
     4921 cccgaatact ctcagtggaa gaaagcccac gaataaaagc aaaaagcaga aagagttttc
     4981 tctttctgct ttattgttta tatctaaaat cgacttagct acaaaacaac ttccgcgtgg
     5041 cgggtaacat ggcagccgat gttcaccgca acgggcagcc ccgcgatatg tgtcggggcg
     5101 gtttctatat tcaccgcaag ggcggtagtc ttgcctccaa atccctgggg tcctatgttc
     5161 agctcgttta tgcgctttaa aagctcctcc tccatctctc tgtaaaactc atcggggtgc
     5221 cgtatgtcga cgctcctgca aagagccttt ttggaaagga tggcggaata ctcaaaatcg
     5281 ccgcctattc ctactcccac cacaatgggc gggcaggggt tggagcctgc ctttttaacg
     5341 gtgtcaataa caaactccat aatgtcctct ttagtggagg tggggagcat cattttaagc
     5401 gcgctcatat tttcgctgcc aaagcccttg ggggatacgg tgagcttcag tttgtcgccc
     5461 gcgactattc ttgtgtgaat aacggcgggg gtgttgtcct cggtgttaac tcgccttaaa
     5521 gggtccttta cgatggactt tctcaaaaag ccctcgatgt agccctgcct tacgccctcg
     5581 tttatggcgg tttcaaaatc cccgcccacg atgcggacat cctgaccgac ctcggcaaag
     5641 attacggcca tgcctgtatc ctgacaaatg gggacactca gctcctcggc agcttttaag
     5701 ttctccgcaa ggtcgcccat aacgcctttg gggagctgtt tttcttcggt ttgataggct
     5761 tcttcgattc ttctttgaag gcaggcgggg agctttaaat ttgcctctat gcaggcgtcc
     5821 cttaccgcct ttgttattgc ccttgcgtca atctctctca tattcttcct ctctttcccg
     5881 aaaggggctt taagccttgt ggacaatctc gccgtttata actaccatct tgggcttggc
     5941 gcttatagtc agcgggtcct ctgagaaaac gctgaagtcc gcgtccttgc caacttcaat
     6001 tgagccgact ctgttttcaa tgcccgccat tttcgccgcg tctattgtga ttgccctcag
     6061 agcgtcctca tgcgcaagac cctcccttgc ggcaagaccc gcgcaaaggg gcaggtactg
     6121 tatggggatg acgggatggt cggtgacgat agagatttca atcccagcct ttttaagaat
     6181 agcgggggcg gcgggggtaa ggtctttaag ttcgggcttg cttctgtcgc aaagcagcgg
     6241 cccgctcata atctttatat tttcctttat aagcctctcg gcgacaagat gaccccttgt
     6301 gccgtggacg attatgtaat caaagccgaa ctcccttgct atcctcgccg cggtcagaat
     6361 gtcgtcctct ctgtgggcgt ggatgtaggc cggaatttcg cccttgagaa gggggataag
     6421 agcctcaaga gcctgattgt agtcctggtc ctcatccttt tcctttttag caaggtattc
     6481 tttcgcctca ttgagaattc cccttatcgc cgcggcggtc gccatgcggg aggatgggct
     6541 ctccttgcgc tcgtggtaga cggttttggg gttctcgccc agagccatct tgatggaaag
     6601 gggagccttg ataatcatgt cgtcaacgca gactccgtgc gtttttattg cggcggtctg
     6661 accgccaatg gggttggagc tgccgggaca agcgacaacg gcggtgacgc ccgcttgagc
     6721 cgcctctttg aaagaaaggt caaggggatt gatggcgtca atggctctga gatgcggggt
     6781 aagggggttt gtcatctcgt ttatgtcgtc ggactcaaag cccagagcgt cgcccacaag
     6841 accgagatgc gtgtgggtgt cgataaagcc gggatagacg ctgtcgcctc tcaagtcaat
     6901 agcgtccgac ttttcgcttt cggaaagctc agatacgctt ccgacccttt taattgcgga
     6961 gccttcggtt tcgataaagc cgttttcaat aacggaacct gccattgtat gtatcacagc
     7021 gtttataaaa agcatatctt cctacctctt aatatacggg tttttgtact tagctttaaa
     7081 tgcattctca aatattgtat aacttaaatc tgctccttgc aagtgggagc aaaacgcaga
     7141 aacggcggaa agggctgtcc gcgccgaaac agcggggagt tatctcacaa tgtgtttttt
     7201 tttgggacaa aatccgccca ttcttagtct aatacgcgaa aggtcccgaa aaagaaaaaa
     7261 taaaagagcg gcgaaccgct ctgtaaaaga cgcaaaaaaa ctctcctcgt ttattcgggg
     7321 tttttttgtt tatctgcctc gtcttgagta tccgccgccg cgctttgact cggtgtagcg
     7381 tttgagggcg gacattttct cctcgcttgc ctgcttgaac ttggccatca tctgctcaaa
     7441 ggactgattc tcagactgct gctcgccgct gtccgcgctt ctttcagcgt tgtttctgaa
     7501 gccctgatgc tgatgacctc tgtttctttc ctcaggcggg agcgcctgct tgatggaaag
     7561 gctaatcttt cctccttctc cgaaggcaag tatcttaacc cttacctcct gattttcgga
     7621 aaggtgctcc ttgatgtcct taatgtagga tgtggatacc tcagagatat gcaccattcc
     7681 cgtttctccg ccggcaagct ttacaaaagc gccgaagttt gtaataccgg tgactttgcc
     7741 ctcaacaatg tcgccaacct gtaattgcat aaaagatgtt ttcctcctat taaaagcccg
     7801 gcatcaaccg ccgggagtga tgtcgtaata aactctctcg tcagggtaaa catagcccct
     7861 gttttcacgg gcgtattttt caatcagctc tttttcgttg ccatcggcaa gaatcctgcg
     7921 cttttcctcg ttttcaatct gctgctgctg ataagcctga gataaggatt ccgcctcgtt
     7981 ctgctttgcc ttaatgtcgg agctgagccg aatcattgaa aacacaaaat agcagacacc
     8041 gatgaggccc acaaacaaga ggataagact atgctttctt atgccgtttt ttttcataat
     8101 ctttacttcc ttttttgctg cctttttttc tcggtcccga attttgttta gccggaggcg
     8161 acatttgcgg caattcaccg cgcatgaaca tttccatttc ctgagcgatt ctcgccctgc
     8221 gctcttcaag gctcagcggt ttcggcgggc cttcagccgc cgcggcggct tttcttcggc
     8281 taaagtaaaa tccgaataca ttatacaata gtgctttaat tcgttgcaag cgtaaaatta
     8341 atttattttt ggtttttatc gccgtttttc taagagccgc gccaattttt tcgcataatt
     8401 tgcgaataag gccgaaaagc tttataaagg ggtaggatat tatcctgaaa actttaatga
     8461 aaagcctcct taaaaattcg attatcctat ccccaatgct gtaaacaaag ctgccgaaag
     8521 taaggtagta gataataaag ccgagaagct cgcccagaac ggcgaacatt ctgatttctc
     8581 ccatgttctt ggagagcaga aaagcgaaag agagggcaaa aacaaaaagg atgtatataa
     8641 catcctgtat ggcaacggcg attttgccga aatttactgc gaggcgcagt attctgaata
     8701 tatcgaatac cgcgcccgca aaaaatccta tgccaaccgc catgaaaaac agtcttgtct
     8761 ggtgcgcaag ctctattccg tacactatct gaacaccttg ctgaagaagc cgccgcttct
     8821 cttttcgatt tcggaataaa gaagggtcga aatgctgccc tctatgcaca tttctcccgt
     8881 ttctatgctg agattattga tatgaaggtc ctgcccctgg atagtcagct cgcccatttc
     8941 cgtattgaca attatcgtgc tttcgtcaaa gctgtccaca tccaaaacgc cggagatgct
     9001 gagctttctt ctgttttcaa gcacaaggtt atggggcatt cccgttcttt tctcctctgc
     9061 caaggcactc acctctaaat tcgcaaatat cctcttttaa ttgatatgag gacaagggga
     9121 ttaatatgcg aataagcttt ggcggagtgt ataaaaagtt acagtattaa taaattgttg
     9181 cataaatgtt gcaaatatta tgtatttaaa gtataattga gcccgactga tttgtatttt
     9241 gtagattttt gaccgcttgg gaggacatta tggttaaggt aagcgtagtc gttcctgtct
     9301 ataatgtgga aaaatacctt gccaaatgcc tccaaagtct tatcggtcag acgcttaaag
     9361 acattgaaat tattgtggta aacgacggct ctgccgacgg ctcgcagaaa atagccgatg
     9421 agtttaagga aagatatccg gaaataataa aatgcgtcgt caaggaaaac ggcggactcg
     9481 gttccgcgag aaacgcgggg cttgaaaggg cgtcgggcga atatatagct tttatagaca
     9541 gcgacgacta tgttaaagcc gatatgctgg aaaggctgta tgacagcgcc aaaaagcacg
     9601 acagcgatgt agcaatcggc ggcaacatct atgttgacga aaacggcgat gttctcagag
     9661 aggagatttt ctcctacggc aaagagtgct ttaacgccga tgaaacaaga gatgtgcttt
     9721 taagctcaat cggcgtctgc aataagatat acagagcgga ttttttaaga aaaatcggcg
     9781 cgaaattcag agttcaaaaa tggtacgagg attttgattt tataattaag gtgcttttgt
     9841 cggctcagag gatttcggtt gtggaggacg ccttttatta ctaccttcaa agacaaggct
     9901 ccataatgaa taactctaac cttataagaa atcttgaaat tcttgaggct ctgagcgaaa
     9961 ctcttaaatt ctgcaaagag cggaatctgt tggaaaaata ttatgacgaa atagagtttt
    10021 tggctctgct tcacgcttac agcgccgcga gcgtcaggat aatcaacgcc gacgcggata
    10081 aaaaggacaa gctcttggcc cttgaggaaa ttaaatccta catggataaa gagtttaaag
    10141 attttaagaa taacaaatat atagggtctc atttgtcaag gaacaaaaag atagtgatga
    10201 gacttcttga tttaaagcta tatacgctta taggccttat gttcaaggca aaaagggttt
    10261 tggcaagata gacggaaaga ggaagaaaat gcagcttatc aaagacagaa aaagacttgt
    10321 tacgggcttt attactgtat ttgttttgct tcagcccttt atagatgtat ttacttcatt
    10381 agccataagg aatagcatgg gcgcctcctt gggcgttttg gtgcgctcat tttttatggc
    10441 ggtaatcacc gcgtactgta ttcttctctc caagaccgac aaaaagctta aactgggcgt
    10501 tgccctttac atgatagcaa ccggcggata ccttatgttc tacttcgccc atttctttaa
    10561 gacgggcggt cttttctttg cctttaccaa cataaaagag gcggtaaagg tattttattt
    10621 tgtatttctg cttttgggct tctaccttat ttacaaggag tacaaattca ccgtcagcga
    10681 cacggttctg tctattacgg cggctctgta tatgagcatc atcgtaatag cctttgttac
    10741 cggcaccagt tacccctcgt acaagcatgg agagggtttt gtgggctggt tctactccgc
    10801 caacgagatc ggagccataa tcggcatttt ggcgccgtat ctgcttgtga attccgttaa
    10861 gggcttaatc aaggctctga aagagaaaaa gtatcctctt tttgcccttt attctttcct
    10921 ctgccttgaa accgtattct gcgctttgtt tataggcaca aaggcggtat tcctcgcttt
    10981 catgcttttc gtcgccattg ctttggcgct ggcgcttttc aaggcgtaca agaaggcaaa
    11041 aaccgtgaat ataagccgaa aggccgtaag ctctgcgctt atcgcggcgg ttctttgcgt
    11101 atctgttttc agcggacttt atatcgtatc ccctatcaga gccaacttaa cgggcaaggc
    11161 agttgtggaa tatacctatc ttcaaaaagg aacgcccttt gaccccgtcg gcgaagaccc
    11221 cgtagagctg cccggcggct ttgagcccgg cgaggggaat gaacagaaag acgcggagct
    11281 ttataccgtt tgggatgcag aaaaggagcc tctgcctgtc cctgtttcgg ttcttaacaa
    11341 ggttttcaat aaccgcattg ttttctcaat ccccgtatca aggcactaca tgagcagctc
    11401 ttgggttgaa aagctcttcg gcgtgggcta tgtgaacagg tcagacgcgg tctacgatat
    11461 tgaagaggca atagaaatcg acattgtaac cattttccta aggcacggcg ttgtgggctt
    11521 tgtcctctac tttgtgccgg ctttcaccct tgctttctgg gctataagca ggattttctc
    11581 gagcaaaaga gatataaaga aacgggactg tgttcttgtg tcccttttcg gtattgccgt
    11641 agcctttggt atgtccgtcc tcgcgggtca tgtgctcatc gcccccgctg tcagcgtcta
    11701 tatcgcaata gccttcatta aactctgcgc caatgccgag aacgcggagg agcacgatat
    11761 gaccgaaatc gtggacaaaa gcctgtcatc ctgccaaaag gaacctcaga ctctttaaca
    11821 aaaattttac ataccgactc aaaggcccgt cagggtcttt tttgttagag ggacttttgc
    11881 cttttatttg ctccgaaaat ttcaaaaaag ctcgcaaacc cttgaagttt taagctttgc
    11941 cgaatatgca ctattgcaaa tgcatatcgg gaatgataaa ataatctaat acagaaagga
    12001 ctgatacatt tgccccgcag aaagtggatt acagccgaaa ccgacaaaga aaaagccgcc
    12061 ctgctcgccg cggaaacggg agcggacagc cttgctgctt tgattctgct ttcccgcggg
    12121 tacgacactc cccagaagat acgggacttt ttaagcggca cggggctttc aagcccattc
    12181 tgccttaagg atatggacaa ggcggtagag agaatactca gagccgtgga taactttgag
    12241 cgcattaccg tttacggcga ctacgacgcc gacggagtta ccgccacagc catactctat
    12301 tcctatcttg accttatggg ggcaaatgtg gactatgtaa tcccctcaag ggagtccgac
    12361 ggatacgggc tcaatttcga gctgattgac atgataaagc agaggggaac aaaccttatt
    12421 atcaccgttg acaacggcat taactccgtc aaagaggcta agtatattaa agagctgggc
    12481 atggacctta taatcaccga ccaccatatt caaagcggcg aattgcccga cgccgcggcg
    12541 gtcgttaacc cccacagaac cgacgaaacg ggtcagcttt caatgctttc gggagcgggt
    12601 gtagcgttta agcttgtctg cgcccttgag ggcgaggaca cagagaacat gctccaggac
    12661 tacgctgacc ttctcgccat cggcaccata gccgacatag ttcccctttg cggcgagaac
    12721 agagctttaa ttgccgcggg actcagatgc ataaacgagg ctccccgccc cgccatagag
    12781 gcgattaggg ctgcctccgg ctttggcggc agatatttca gctccagagc cgtagccttt
    12841 accatagctc ccagaattaa cgccgcgggc cgcatgggct ccgctgagag cgctttgaaa
    12901 ctgcttctca gcgaagacta tgacgaggcg ttccggcttg ccgaggtttt ggaaaaagca
    12961 aataaagaca ggctcgaggc ggagaaaaga atatatgatg aaatagagcg gattttcgat
    13021 aaagacccct cgctctatca ggacagagtt atagtggttt cgggcagcgg ctggcacccc
    13081 ggggtcatag gcatagtagc cgcaagatta tgcgaaagat acggcaagcc cgccgttgta
    13141 atatcggttg acgaggacaa tataggcaga gcctccgcca gaagcattga gggcttttcg
    13201 attttcgagg ctttaaaggc tacgggagat ttgctgagcc actacggcgg tcacaccctg
    13261 gcggcgggtt ttacgataga agaaggtaaa ataagcgaat tcagaaaagc cgtaaacgag
    13321 tacgccggaa ccgttaaaag agcagtgccg accctcttta tagactgcag gctgaatccg
    13381 aaatacataa gcaattcggt tcttgaaagc cttgagtttt tagagcccta cggagcgggc
    13441 aatcccactc ccgttttcgg gcttttcgga atggagattc agtcggtgcg ccccgtggga
    13501 aacggtcaca taaggctcct tttatcaagg gacggcacaa gaattgacgc cgtcaagttc
    13561 ggcgtaagcg tcgacgagtt cccatacgcc gtcggcgaca gggtggacct ggcggtggtc
    13621 atagaaaaga atgaatacat gggcacaata agacccagca tacaaattaa agacatacgc
    13681 tttgctggca ccgacgacga ggcgctgttc gagaatctta taaagtatga ggagatatcg
    13741 agcagagagg cgagcgtcaa ggaaaaatgc tccccctact gccccacaag ggagttcatg
    13801 ggcaatctgt tccgcttttt aagggcaaac ggcggctgga ggcacgacct tgaaatactc
    13861 tcctacagaa tgggtcttga gccgtcggaa atagtaaaac tcgggctcgc ccttgacgct
    13921 ttgtgcgagc tgcagctcat ttaccgctta aagacaggcg gctacgccat ggcggaaaac
    13981 cccaaaaagg ttcagctttg ctcctcaaag atactgacaa atctcggcta tacagaataa
    14041 ataaaaacgc gcgaaaggga ggtgagcgca tgactgaaat cagcatggaa gaaaaaaggg
    14101 cctatgagag ctttatctca aaggtacggg aaaactactc aaaagaggac gcggacttaa
    14161 tagatctggc gtacagaaag gcgtccgccg ctcatatagg acagacgagg gaatcgggag
    14221 agccgtatat aatccatccg attgccgtcg cggaaatact catgggcctc ggcatggacg
    14281 ctcagtccct tatcgcggct ttgcttcacg atgtggtgga ggacaccgat gtctgcattg
    14341 acgagatagg ctgcggcttc gactccgatg tggccgctct cgtggacggg gtcacaaagc
    14401 tcaacaaggt gccccttgcc agcaaagagg aggcgcaggc ggagaatata agaaagatgc
    14461 tccttgccat gtcggaggat attagggtga ttatcataaa gcttgccgac aggctgcaca
    14521 atatgcgcac cctctctttt gtaagcgagc agaaaaggcg cgacaaggcg ctggaaacac
    14581 tcgaaatcta cgcccccatc gctcaccgtc tcggtatcag acctattaaa gaggagcttg
    14641 aggacatagc catcagctac cttgacccga ttgcctacaa tgacataaaa gagtcccttg
    14701 aggctcagag caagtcaagg aacgaattcc ttgaaaaaat caaggcgaga atcggcgaga
    14761 gaatcagcga aatagtaaag ggcgcgaaga ttgaaggacg aataaagagc gttcacggca
    14821 tatacagaaa gatgtatatg cagaacagga cctttgacga gatttacgac atttacgccg
    14881 taagaatcat agtaaacacg gtcatagact gctacaactg cctgggcgtt attcacgata
    14941 tgttccgccc catccccggc aggttcaagg actatatttc aactcccaag cccaatatgt
    15001 atcagtccct ccacacgacg gtcatcggca aagagggcat cccctttgag gttcagataa
    15061 gaacatggga aatgcaccgc accgccgagt acggcatcgc cgcccactgg aaatataagc
    15121 tgggacttaa gggcaagcag cagtttgagg aaaggctcgc ctggataaga cagcttctcg
    15181 agcttcagaa ggactccgag gatgtggagg acattgtccg caccattaag accgaccttg
    15241 ttcccgagga ggtctatgtg ctcaccccca agggcgatgt tataagcctg ccgcagggag
    15301 ctacggtcat agactttgcc tacgccattc actcagccgt cggcaaccgc atgatcggag
    15361 ctaaagtgga cggcagaatc gtgcccatta cccatgtggt caaaacgggc gagattatag
    15421 agattctcac ctcaaatcag accaagggcc ccagccgcga ctggctgaaa atcgtcaaga
    15481 ccagcgaagc cagaagcaaa ataaggcagt ggtttaaaaa gcagaacagg gaagagaata
    15541 tcgaggtagg caagtccgag cttgaaaggg aattcaggcg cagcaatata aggctcgaag
    15601 aagagggaat gaacgagctt atacaaaaga ttgccgaaag atacaatttt caggatattc
    15661 aggacttcta cgccgccata ggctacggcg gagtcagcat cagccgcatc ctcccgcaga
    15721 ttaaggagga gtacaacaag ctgattaagg cccagtcgcc cacccccgta aagctcaaaa
    15781 tgaagagcgc agcgagcagc gagggcgtta tagttgaggg cattgaaaac tgccttgtca
    15841 agctttccaa atgttgcaac ccaatccccg gtgatgatat aataggtttc ataacaaggg
    15901 gtcacggcgt gtcggtccac aaaagggact gctccaatgt tccctatccg attgagtccg
    15961 ctcccgagcc cgacagatgg attcccgtta ggtgggattc ggacatcaaa gaggacttca
    16021 acgccgcact ccttatcacc tgccttgcgt cggtgtcaat catggcggac atatccggcg
    16081 cccttgccaa tatgcatgta atgattcact cccttaacac aagggagaca aaggacggca
    16141 ggtatttggt caccgtcacg gtaaccgtta agagccttga gcatttaagg agcgttatgt
    16201 caaggcttga caagataaac ggcgtattca atgtggagcg ttccggtctg taaatttttt
    16261 ctaaagaaag gaaacctgcc atgcaaaaga agctttaccg ttcaagaacc gacagaaaaa
    16321 tcttcggagt ctgcggcgga atcgccgaat acctcaatgt tgactccaca atgataaggc
    16381 ttgtttgggt gctgcttatg attttcgcgg gaacgggtct tcttgcctat atagttgccg
    16441 ccatagttat gcccgaagct cccgagaatt ccaacaacaa cccctacaac aatccctatt
    16501 ccaattacta cggtccctat aataacgacc cctacaacca gaacggctgg caggatcaga
    16561 gcaagggctc taacccctat gacaatcccc ccgagaatat gcgttagctc taagggggat
    16621 atatgaaggc tgtaatacaa agggtagaga atgccgccgt caccgttgac ggcagagtag
    16681 tcggcagcat cggcggcgga tatatgattc ttcttggggt tgtggatacc gataccgagg
    16741 cggaggcaag gctgcttgcc gccaaaaccg cctctttgcg cgttttcagg gacgaaaacg
    16801 ataaaatgaa cctgtccatt ctgcaggtgg gcggcgaggc gcttgtaatt tcccagttta
    16861 ccctctgcgc cgacacaaga aaaggcaacc gaccgtcctt tacttcgtcc gcggagccca
    16921 aaagagccaa tgagctttat gagctttaca tggacgagct taggaaaaac ggcgtaaaga
    16981 aagtagaaaa gggcgagttc ggcgctcata tgaaggtaag tcttgtaaac gacggacccg
    17041 taaccattat tctcgatacc gatacatgga aaagggctta aagcgggggt ttttatggaa
    17101 ataatcagca taaagccttt cggcaatttt gcgaacacat atattatcaa agacgagtcg
    17161 ggagcggcgg cgatagtcga ccccggctgt gttacggatg agcttaaatc cgaaatagaa
    17221 aaatgcggca aagtagagta catccttttg acccacggtc attatgacca catctggtcc
    17281 gcaaaggaga taaaagagct gacgggggca aagctcgccg ttcacaaaaa cgatatgccc
    17341 ttccttgccg atgagagtct cagccacggc tgcaaatact tccccggcga gcaggatgtg
    17401 ccgcagcccg acattgtctt agagggcggc gaggttctga aactcggcaa ttcggaaatc
    17461 aaggtcatga gcacacccgg gcatactaag ggcagcgtgg tttatatagt cggcgacaca
    17521 atgtgcgcgg gcgacaccct cttttatctg accgtcggac gcaccgacct tgagggcggc
    17581 agcatggagg atatgataaa cacccttgag aaactaaaga atcttgaggg cgacttcaga
    17641 gttctccccg gtcacggcag ggaaaccact ctggagcacg aaagacaaaa caaccgctac
    17701 atgtcaaaac ttgatttgct gaaagagcag tatctcggct aaaggaaaac tatgaagctt
    17761 tatttaaacc acccctttca ctatgaggca gaaaacctta cccgcctctt ttttccttcc
    17821 gaaaaaatac aggtaatacg agagcccttt attgagggag aaagcaaagc tccgtcggaa
    17881 aattccgacg gctctgaagg ttttattgtt tcttcccttc aaaaggcgga cggctcatac
    17941 aaaatcacag catatgtaaa agtcggtgaa agggaaagct atgatgaatc ttttgtatcc
    18001 gatactttgg ccgaaaaggg cttttttact gaggaaaagc agggcgagga aagggacaaa
    18061 gcggagcttt tgatggctca gtgcttatac aaagcccttt cggagcttac ctcatacacg
    18121 cccccgtggg gcatattgac aggagtcagg ccctcaaagc tttacaggag cctttatgaa
    18181 gaaaagggct atggggcctt tgattttttt aaaaacgagc ttttcgtgag cgagaataaa
    18241 ataaagctcc ttcacagtat tgtaacgcgg caaaaaggaa taatagattt atccaaagaa
    18301 aactcctact ccctttatgt gtccattccc ttttgcccaa caagatgcga atactgctca
    18361 tttgtctccc attccatatc ccactttaaa aaaatacccg aatatatcgg tttgcttata
    18421 aaagagcttg aggaaatatc gaaaatcgca agtgagcttg ggctgaagct cgaaaccata
    18481 tatttcggcg ggggtactcc cacaagcctt aatgaagctc agctaaagag cgttaccgac
    18541 gcggtggcgg ctctgttccc ccttgagggc gtcagagaat acaccataga ggccggccgc
    18601 cccgatacct tgaacgagga aaagctccgt attatgaagg cggcgggggt tacgagaata
    18661 agcattaacc cccagacctt caaggacgag attctgagaa aaatcggcag atgccatacg
    18721 gcggcggaaa caattgacgc ctttaatatg gcaagacaaa tgggctttga caatattaac
    18781 atggacttaa taacgaatct ccccggggac actcccgagg gatttatgtt cagccttaat
    18841 aaaaccatgg agctgaatcc cgagagcgtt actgtccaca gccttgcggt aaagcgctcc
    18901 tccgcccttg caagaaccga gggcattaac gccgagtggg agccttcggt ttacgaaaac
    18961 gcctacaaag cccttgagga aaagggctac aacccctatt acctctaccg ccagagcaag
    19021 tcggcgggca acagggagaa tacgggcttt tcaaaagagg gctttgaatc cctttacaac
    19081 atctacatga tggaggagac ccacaccgtt ttggccgcgg gagcgggcgg cgtttcaagg
    19141 ctcaaagagc ctaacggaga atttatagac agaatcttta actttaaata tccctatgaa
    19201 tatgtttcaa actttgagga gattttaagg cgaaaggaca aaatcaaggc attttacgag
    19261 ttttaagtca cgcaataaag gaaaacgcgt aaaatggtct aaaagccttt taaacttcgg
    19321 aggaaatatg cagaatttat gcaattcttt aagcataatt aataataata taagggacaa
    19381 tcccgacgcc tttatttttg agtccgaaaa gttttatcac gaccagctta aaaaggtcat
    19441 tgagcttatt aaagacagga gcggcaggga aatcataatg cttgccggcc ccagctcatc
    19501 gggcaagacc acgaccgccg acaagctgct ctccgccctt acaaacggcg gcaggcaggg
    19561 ctatgttatt tcccttgacg acttttacct caacgcgggg gagggtccca gaaaagagga
    19621 cggaagtttt gactttgaaa gcgtccatgc cctcgatttg ccccttattc attcctcaat
    19681 tgagcagctc ctttcgggca aggaaacaaa gctgcccctt ttcgacttta ccgtcggcaa
    19741 gcgcagcgac aagacaagga gcgtcacttt aagggagcac gactttgtca ttatcgaggg
    19801 acttcacgcc cttaaccccc ttataacctc ctccttttcg tccgcgaacc ttataaagct
    19861 ctatataagc gtgaactctg acatatatga cggcgatgat gttcttttaa gccgcattga
    19921 gctgagattc ttaaggcgtc ttgttaggga ctacaaatac cgcaacagct ccgcggacaa
    19981 cacttttatg ctgtggacgg gcgttgtgga gggagaaacc aagtaccttc tgccccacag
    20041 agagaacgcc gatatacgcc ttaactcctt ccacccctat gaggtttgcg tattaaagga
    20101 tatggcggct cctctccttc aaagcgccgt aaaagaggag tacaagaaag aggcagtcag
    20161 gctgctaaaa agcatttcaa aattcgatac ctgcccctgc ggcaaggtgc cggcggattc
    20221 tctcctgcgc gaattcatag gtcccatgga agaatgcaag ttttaaatca caaaggaaac
    20281 tttgtagaaa tatacgagag agggtttaat tttggctgaa agtggaatga agcgtcaatc
    20341 tctgcttaac ggagctcttg tgcttgtaat cgcgaccgcc cttgtaaagg ttataggggc
    20401 gctgttcaaa atccccataa ctaatcttat aggacccgtg ggcaggggct atttcggctc
    20461 cgcctacaat atcttcatac ccgtctacaa catagctatg gcgggtctgc ccgttgccat
    20521 ctcaaaactg gtggcgcaga acatagcttt gggacgctat cgagatgtaa gactgatttt
    20581 taagatcgca aagagattgt tcttccttac gggcgttgtg ggtatgctga ttctgatgat
    20641 tcttgcctac ccgtacgcca aatcgattaa cacaactcag gttataccct ccattctcgt
    20701 aatcgccccg tcgatattct tctgctgcat gatgtcggca tacaggggct attattcggg
    20761 actgagaaac atgaacccca ccgccgtgtc ccaggtcatt gaggctttgg gcaagctgat
    20821 tctcggtact ctgctcgcca agttcaccat ggaccacggc atgaacaact tcaaagaggg
    20881 caaaaaggtt ttcggcaaag tggtcacgac tgaggcggag gctctcagcg ccacataccc
    20941 gtatgccgcc gccgcggcag tggcgggcgt tacaatcggc acggtgtttg ccctcggcta
    21001 tctgatactg aggcacaggc tcaaaaagga cggcatctca aaggctatgc tccgctcctc
    21061 gccaaagccc ctgcaggtca aaaaccttac ataccagctc attggtatag cgattcccgt
    21121 ggtggcaagc accatcatca tgaaccttac aaactttata gattcatggt caattcagtt
    21181 cagactcagc cacgctatag cctccaatga agggcttata agggctatgt acgaaaccgc
    21241 ccttaacacg gcaaacattg tgacaagcga ccttaaaacc tatctctacg gagcttatga
    21301 gaccgccctt gactttaaga acctcatccc cgcctttacc atgactctgg gcgtcagcgc
    21361 cattcccgtt ctttcagagg cctggacctt aaagaacaaa agggagataa aaaaggcggt
    21421 tgagtcggta ataagggtta ttatgcttat cgccatgccc gcgggcatcg gaatggcggt
    21481 gctggcaaag cccatactca gcatactcta caaggatatg tcctccctgt ccattgccgc
    21541 tcccgtaatg gcatactacg gctacgccac cttcctcata gccttctctc agccgcttac
    21601 aaatatgctg cagggcatcg gcagaatgga tattcccatg aaaacacttg ccgtagccgc
    21661 ggtgttcaag atactcataa actttatatt tgtgggcata cctaaataca atatattcgg
    21721 cgccgttata ggcacagtgg ttttctatgt gattatcgtg gcgggcggaa tgttcttcct
    21781 aatccgcgaa acaaaggtta agctcaatta cttcaatatc tttataaaac ctcttttctg
    21841 ctccgttctc tgcggagccg ccgcatggtc ctcccacgga cttttaagca tggttcttaa
    21901 attcggcgac ccgtcatcaa gactcaacgg agcgaatgtc gccacgcttc ttgccatagg
    21961 catcgcagtg gtggtttacg gtctttcact gcttttcagc agaacaattg cgaaaaccga
    22021 cttaaatatg ctgccgggcg gagaaaaaat tagcaaaata cttgaaaaat atggatttat
    22081 cggttaatat aaaagcaatt acagcgaatt gtactaatat ttagcctaaa atccaaaata
    22141 tgtgagcaat ttgctagttt cccgccgtga aagggaagtt gaaacgcttg aattttgagt
    22201 ttaaggacaa atacaatata aacgaccttc tcagaataat ggaaatcctg cgttcacccg
    22261 aaggctgccc ctgggacagg gagcagaacc accggagcat aaaaaagaac cttatcgaag
    22321 aaacctacga ggttgttgag gccataaaca aagacgataa ggagcttttg cgcgaggaac
    22381 tgggcgacct gcttttgcag atagttttcc attctcagat ggaaaaggag cagggcgtgt
    22441 ttgactttga ctctgtctgc gacggcatct gcaaaaagct cattgagcgc cacccccatg
    22501 ttttcggcac tctggaggta aaggacagca aagaggtgct ggagaactgg gaacagataa
    22561 agcgcagaac aaaaaagcgg gacaccgttg ccgacgctct ggaggctatc cccaaggagc
    22621 tgcccgccct tatgagaagc gacaagatag tatcaaaggc tgaaggctca tatttcgaga
    22681 aaaagggcgt aaaagaggcg gcgaaagccc tctgtgactt tgcgcgggat atcgagaacg
    22741 aggacaagcc cgcggaatcc gccgaatttg tcggcaaact cctgtttgaa gctgtacgcc
    22801 tttcaagggc gcttaaagtc gacccggagg acgctctggc aagatattgc acaagccttg
    22861 tggaagaatt aaaggcggca aagcccgaga aataaataat cggcatagaa ttggcaatac
    22921 tttctgttga aacacgacga ctccaacagc cgaaaggcgt ttggataaat ttttaaggag
    22981 gaaattcaat gaataaatcc gatcttatca atgcagtagc aagcaaagca gagcttacaa
    23041 agaaggatgc ggaggcagct gtttctgctg ttatctcctc tatctccgaa gctctcgcat
    23101 caggcgaaag cgtacagctt gtcggcttcg gtacctttga ggttcgtgat cgcgctgcta
    23161 agcagggtcg caatcccaag acaggcgagc ccatcacagt tcccgctgct aaggttcccg
    23221 cattcaaggc aggcaaggct cttaaagaca gcgtaaataa gtaattttac atgtgctgtt
    23281 ttttaaagcg tgttgtctga agttcgacaa tgcgccgact gaaatacgga ttcagcagga
    23341 tttaacggcg gacaagctat ggcttatctt gccgtttttt ccttatgtat aaaattttta
    23401 atggagattt aagcctttgt gttaatataa aaatcggagg ataaaatgcg acttgacaaa
    23461 tacttaaagg tttcccgcct tgtaaaaaga aggacagtgg cgaacgagct ctgcgacgct
    23521 cagcatgtaa gcgtcaacgg caaagtggcg agagcctcct atgatgttaa ggtgggggac
    23581 atgatagaaa tcagaacggg caataacctt attaaagcaa aggtgcttca ggttaccgag
    23641 catgccacaa aagaaaacgc ctcccagatg tatgagctta taaaataagt gcttgccgaa
    23701 aggcggaaag cgggtgagtt tatgagaacc gataagggtc aaataaaaat agtggctcta
    23761 attttggctc tgtgccttgt ctgctccggc gtgggcggag ccgcgggagc gctgaccgct
    23821 tacaggattt ttacaaataa gctccaggcg gatttgccca aagagcccga cgagggcgag
    23881 gctctgaccg tacctcccgc tttgacgacc gaggcggagg ttacaaccga gccggttacc
    23941 gaaccccgaa ccgaaaaaca gacagagccg cagaccgaaa ggcaaaccaa tcccgcgccc
    24001 atctcccttt ccgcaaagga gattttcaat aagaccgtat cgagcgttgt ggccattaag
    24061 acagtggtta cattcaaaag ctctggaatt ctcggcaccg tcacaaggga gggcacaggc
    24121 tccggctcgg gctttatata cacccaaaac ggctacatcg tcaccaacta ccatgtaatt
    24181 cagtcagcta agagcataaa ggtgacgctc cacgacaaca aggagtttga cgcgaagctc
    24241 ataggctacg accaggagaa cgacatagcg gtcataaaga tagacgcgga gaaccttaac
    24301 gctgtgaccg tgggcagctc atcggaactc agcgtcggtg acaggctgta tattataggc
    24361 aatcccttgg gcgaactgag tcagaccctc tcccagggcg ttgtcagcgg acttaacaga
    24421 aagatagata cgggcaatta cattatcaat atgcaccaga ccgacgccgc cataaaccag
    24481 ggcaactcgg gcggccccgt ctttgacgaa aacggcaggg tcgtgggcat cgtcgccgcc
    24541 aagtacgcgt cggagaccat tgaatcgctg ggatttttta ttcccattga cgatgttaag
    24601 acgaaactca acgacataat gaagtacggc tatgtaagga ataagccgta tctgggactt
    24661 tcggtccaga cggtgacaaa tcttttgtcg ctgcagtaca ggattcccgt cggcgtctat
    24721 gtggtggacc ttgacaccgc cggaccggcg gcaaaggccg gcgtacaggc taaagatgtc
    24781 ataaccaagg tcggcggcaa ggctgtcacc agcgtggcgg agcttaaaaa cgagctttcg
    24841 aagttcaaaa cgggtgacaa agttgagctg acagtttcaa gaagcggcgg cggcggagtg
    24901 tttaccgtca ctctgggcga gcttgtcccc acaacaaaac ccagaacaaa ttatagtgat
    24961 gtgtatgatg tataaattct ctgccccatg gcgctctgtg tgagcggggc agtaggcgac
    25021 cgcccgtcgg ggaatccccc gcgcagccgc gcggcgggtg agagaagtcg agcagcggct
    25081 cagcccccgg ccacgcacgg gtgtaataca tatcgggcgg cagaaacagt gaaaagcgat
    25141 attaaggaga gaatcatttg tcttttgaca gttccttttt gaatacggaa aaatcagacg
    25201 gcgggagagc ttggcgcatt gaggctcttg gcggcggggt gtcggcggtt gtgtcaaggg
    25261 agcacaactt cggcaccgac gcgctgcttc tcgcctattt tgccgagccc aaaaacatag
    25321 acagagtatg cgatttgggc acgggctgcg gcattatccc cctttactgg cgctcccgcg
    25381 gcgttaccgc ctacatagcg gcggtggaaa ttcaggagtc ggcgtacgct caggtctgcg
    25441 aagctaacag aattaatcct cccgacaaaa gcgtggtgcc cgttctcgct gatttaagaa
    25501 atctcaaagg catactcccc tttggcgagt tcgaccttgt gaccatgaat cccccgtact
    25561 ttaagggcgg gattatgtcc gaggataagg agagaatgac cgcaaggcat gaccttgagt
    25621 gcacccttga cgatgtgtgc agggcggcgg cgtcgcttct gagattcggc ggaaggttct
    25681 gtatgtgcct gcctcccgaa aggctctgcg acgctgtgga gctaatgcgc aaatactccc
    25741 ttgagccgaa acgcctcaga ttcgccaaat acaaggaggg cgacaagccc taccttatgc
    25801 ttattgaagg cagaaggggc ggaaagccct ctcttgcggt tctccccgac attgtgctca
    25861 ccgaaaacgg caagggggag tcgaaagaga tgaaagaggt gctctcagag taccttaaaa
    25921 tgaaggcatg caacacgagc gggagaaaag aaaatgagcg gtaaacttta tatagtcggc
    25981 acgcccatag ggaatttggg cgatatatcg gaaagagccc tggcgacttt aaaagagtgc
    26041 gactttatag ccgccgagga cacaagggtg actttgaagc tgcttaacca ctttgacata
    26101 aaaaagcctc ttgtcagcta ccacgagcac aacaagcgcg agcggggcgc aatggtcgcg
    26161 gacagaatct taaagggcga aacctgcgct ttagtcagcg acgcgggtat gccctgcatt
    26221 tcagaccccg gtacggagct tgtaaacacc tgcatagagc ggggaattcc cgtccttgcg
    26281 gtgccgggac ccagcgcggt cattaccgcc ctggcagtca gcggtctgaa tgtaagccgc
    26341 tttacattcg agggattctt aagcgtcaac aaaagcagcc gcaaaaagca ccttgaggag
    26401 ataaagggcg aaaagcggac gatggtcttt tacgaggcgc cgcacaagct gccgaggaca
    26461 ttgaaggatt tatacgaaac cttgggcgac aggcgcatag ctcttgtcaa ggagctaacc
    26521 aaaatctacg agcaggtgga gcgcaccacc cttgccgccg ccgctgagaa atactctcag
    26581 caaaagccaa agggtgagtt cgtgctcata atcgaaggaa gagaagaact caaaagcgaa
    26641 accgagccat tgagcgcaga ggatgccgcc gagctggcaa ggcagtatat tgaggagggc
    26701 ttgagtccca ccgaggcggc aaaaaaggcg gctaaagaat cgggacataa aaagaacgat
    26761 atatacaggc tcttaacaag cgattaatcg gggcaagagc ctctatgaaa ggcgtgaaaa
    26821 ctttgggagg agaaatcttt aatattgcga tttcgggtct gtccgagcgg cagatattgt
    26881 atttattgtg gaccgcggcg gcggcgctct cggtcgtcac cgtctttgta ataatcaagg
    26941 cgggcaaggc atacaaaagg cgtcaggaga ttgtgggaga gcagatggcg cagatggaaa
    27001 gggacagagc cctttatgaa aagtacaaaa acgccgactt tgagaccctt aaaaacgccg
    27061 agccgagaga gcttatatac ggtcttgcct tgcaggtgca ggctcaggtt caaaacgctc
    27121 cgaatcccaa tgagctgttt atgagccttc cgagttttca gcgttatatc tacagccttg
    27181 acatttttct tgaggatacc gaaaagtctt taagctcctt tttcagaaac aacggcgagc
    27241 cgctcctcag cagcgccatc gacgccttaa gggaaaccgg gcttttgacg acggaaatcg
    27301 agaggctctg tttaatgttt gacaaagact ctgaggtctc ctttgacgag gagtatataa
    27361 agaaggcgga tgagtcattt ggggatagct ttaacagaga aaccgccctt aaaaaaatca
    27421 aagagtatat tttgagagaa aattctgaat cagacaaaaa ttgttaaaaa atttcaacga
    27481 cggcaaaaat gcttgtaata atttacacca aatgttatca tttaaaagat gctgacctat
    27541 catcgtaaaa caaaggagag ttactatcct tgcgtaggtt tttaaaactg ctgaggccct
    27601 atgcgggact gctggctctg tcgctgctta tggtaattgt ttctaacgcc atgcagcttg
    27661 tgctcccgca gctcaccgcg gaccttgtca acgaaggcat tatgaagggc gaccttgaaa
    27721 ccataaagta caagggcatg ctgatgcttg ccgtttccgc cctcggcctt gtaatctcgg
    27781 tagtaaacag ctactgttcc agcaagacat catcgggcta tgccatggtt ttaaggcggg
    27841 aggtctttca caaggttgaa agcctttccc agagcgatgt ggacaaaatc ggcgtgccgt
    27901 cccttatcac aaggacgacc aacgatatca ggggaattca ggactttatt ctttcgggac
    27961 ttagaatact tgtaaccgtg cccattctat tggtgggcgg tcttgtcatg tccattaaga
    28021 tgaatcccga gctgacaaag attttgctga ttattattcc gataatcggc gttatcgctt
    28081 ttatacttta tttgtttctc tctcccaaat tcgcccttat gcagaaaaag accgaccagc
    28141 ttaacaaaat agtcagagaa aagatttccg gcattagagt tataagggct tttaacagaa
    28201 ccgattatga ggacgagaga tttaaaacag cgaacttaga gctcactgat attagcctta
    28261 ccatcaatcg ctttatggcg gggcttttac cggtgggact tttcatcatt tatggcctta
    28321 tggcaatcct tttgtgggtt tccaccacaa acatagcaaa aatggacgcc gttactcagg
    28381 ctgacagaat cagctccacc ataggcaatc tgcaggcctt catcgtttac atcggacttg
    28441 tggtctttgc catcagcatg gctgcaacaa tctttatagc aatccccaag gcaaggattt
    28501 cattaaagag aatcaacgag gttcttaaca tggagcccgt tgtcgtggaa aaggaaaacc
    28561 ccgcctttat cgctcccgag cacagaaagg gcgtactgga gtttaggaat gttagctact
    28621 cataccccgg ctcggatgtt ccggccgtta agaatgtaag ctttaaatgc agccccggcg
    28681 aggtaactgc ggttatcgga gccacgggca gcggcaagac gacgcttata aatcttatcc
    28741 cccgccttta cgatgtgacc gagggtcaga ttctccttga cggcgtggat ataagagagc
    28801 tgagtttcga cgagctgcat tcaaggcttg cctttatccc gcagaaagcc ttccttttca
    28861 gcggaacggt cgaggataac ctgagattcg gcaaagagga cgccaccgac gaggaaatca
    28921 tgcgcgctct cgaaatagct caggcaaaga cctttgtgga ggctctgccc gaaaagactc
    28981 agaaccttat ttcacaaagc ggcacaaacc tttcgggcgg tcaaaagcaa agacttgcca
    29041 tcgcgagggc aattatcaga ggcgcggata tatgcatatt tgacgacagc ttctccgccc
    29101 ttgaccttac aacggacgca aggctcagag ccgccattaa gcagcatctc agcggcgcca
    29161 acataatcat agtcgcccag agggtcagca ccattcttga cgctgacaga atcatagtca
    29221 tggacaaggg cgaggttgtg ggcatcggca cccatgcgga gctgctggaa agctgtgaga
    29281 tttacaggga cattgtccgc tcccagcact tagaggagga ggtgtccgaa tgagcgagat
    29341 taaagcagaa gaaagacgcc tccaaaaatc ggagaagaaa gtaaaggtca gccgagaaaa
    29401 gctctcccac agatactctg gctatcacag aaaaagaata aatcagcaaa agccccagaa
    29461 cgcaaagggt accttcaggc gttttgtcgg gctgatcgcc aaatacaagt ggcagatggt
    29521 gctcatagcc atagctaccg taataatagc cctgctcaat gtttggggtc ccgacattct
    29581 gggcgaggtc attgacgcca taaagaggca gattgactta aaacttcaga ccaacaccaa
    29641 tgactttacc gagattaagc agcttatttt caagataatg gcgatataca tgggctccgc
    29701 cgtgctgaca ttcattcagc agtacacatt ggcgggggtc tcccagcagc ttgtctgctc
    29761 actcagagag agaataaaca gaaagctctc aaagctgccg cttaaattct ttgacaccaa
    29821 cacaaagggc gatattttaa gccgcatcac caacgacata gacaatgtaa gcaacagcct
    29881 tcagagcaac atggtgacca ttatcaccaa tgtggtgcag ctgattaccg tctttgccat
    29941 aatgctctac accaactggc tgatgaccgc cataatcgtt gtctttgtgc cgctgagcac
    30001 ccttatatcc tacaggattt caaggatttc aaagcgtctt ttcagagacc tttgggacag
    30061 aacgggcgaa ttaaacggtc acatcgagga gatgtatacc ggtcacagca tagtaaagct
    30121 gttcggctac gaaaagcagt ctatcgccga gtttgaccag ataaacgagg acctgatgaa
    30181 aaccgcaagc aaggcgaatt tcatctcggg tacaatcaac ccccttctca cctttgtaaa
    30241 caacatagtt tatgttctcc tgtgctttgt gggcggctat ctggtgctga acaacagaat
    30301 caccctcggc gacatcgccg tattcttcgc gtactcaagg atgttctcag gccccatcaa
    30361 caatatctcg caggtcatca actccgtaca gtcggctctt gcctccgcgg agcgtgtctt
    30421 taatgtgttc gacgaggagg aagaacccgc cgacagcagc aaggctctta tcaaggagcc
    30481 gaaaggctat gtggagttcg agaatgtaag ctttagttat gttaaggaaa agccccttat
    30541 cgagaacatg aacctcaaag tcaaccccgg cgagctgatt gccattgtcg gacccaccgg
    30601 ctcgggcaag accacattcg taaacctcct aatgcgcttt tacgatgtgg attcgggctc
    30661 aatcaagatt gacggcgttg acataaggga tattcccagg gagaacctgc gcagcatttt
    30721 cggcatggtt ctccaggata catggctctt taaaggcaca ttcagggaga atatcaccta
    30781 cggcaaaatg aacgccaccg acgaggaggt catcgccgcc gcaaaatccg caagggtaca
    30841 cgactttata atgtcttacc ccgacggata cgacaccatg cttgacgagg gcggagctaa
    30901 catctcccag ggtcagcagc agcttataac catcgccagg gctatacttg ccgaccccgc
    30961 cgtgctgatt cttgacgagg cgacaagctc cgttgacaca aggacggaga tgcacattca
    31021 ggaggctatg cgcactctga tgaagggcag aaccagcttt gtaatagctc accgcctctc
    31081 caccatcagg aacgctgaca agatactggt aatgcgccgc ggctccattg tggagcaggg
    31141 cacccacgag gagctcatgg cggcgaacgg tttctacgct accctctaca acagccagta
    31201 tgtgggcggc ataccgcctg aggaatcaga ataattcgac cccatcccga aaggatgggg
    31261 attttttatg cccgccccga cagagcatgg cggagagagc gggactccgc cccttgccgg
    31321 cgtggggcga atcggtgttt ttaccgcgct ccttggggcg cgggacagaa caatctccgc
    31381 tgccgcgcgg tgagggtgag tgcaatatct gcggcgggct gccgtccccc gccccattgt
    31441 gtgagtcgga gagccgcctg cggggagcgt actatcccca gcccgccgcg ggcggggcaa
    31501 agtgggtttc cgccaatagc gcgggcaata aaaaagcacc gctttcgcag tgcttttatc
    31561 ggcttattct tattttgaga ctcttgcctc ggcgatgcgc tctattaaga tttttgcctt
    31621 gtttgttata tcctcatagt cgcccttctt cgcgcccgct gtcagtgagc cgccgacgcc
    31681 gactgcgaac gcgcccgctt taatccactg agccacattc tcagtgtcca cgccgccggt
    31741 gggcatgaaa acgccgtggg gcagtgggcc tttgaacgcc tttatgattg cgggtccgaa
    31801 aagctcggcg gggaatactt tcaggacatc ggctcccgcg gacatcgcct gcatagcctc
    31861 tgtaacggtc ataacgcctg ccattacggg taccctgtac ctgttgcaga gtttaactac
    31921 tccctcgtca aagccggggc ttaccacgaa cgcggcgccc gccatcatgg cgacccttgc
    31981 ggtctcggcg tcaagcactg tgcccgctcc cacaagaagg gtctcagcgt catactcttt
    32041 gcagagcctt tcgataacct tctccgctct cggaacggta aaggtaattt ctatggcgtc
    32101 aattccgccc tttatgcaag cctctgctat tttaagagcc tgctcctcgc tctcggctct
    32161 gaccactgcc acaatgccct tgtcgctgat tctctgtaaa accttggatt tttccataat
    32221 caatcgccct ttcttactat cttacaaccc ttgcgcctgc gcccgccata atggcctcaa
    32281 cctctttaag gctagccatg gaggtgtcgc cgggggtggt catggcaagc gcgccgtgga
    32341 caacgccgta gttgactgcc ttctgcgggt cgcctgtggt cataagtccg tagataagtc
    32401 cggaggcgaa ggagtcgccg ccgcctactc tgtcaaatat ctcaagattc ttaaactcca
    32461 tgcccctata aaccttgccg tcggcgtaga ctatcgcgct ccagtcgttg attgtggcgg
    32521 tctttacggt gcgcagagta ttgccgataa ccttaaagtt cggataagtc tcaaccgcct
    32581 tgccgagcat cttgatatag ccctccaggt tcagctcctt taagttctcg tcatttccct
    32641 caatctcaaa gccgaggcag gcggtaaagt cctcctcgtt gcctatcatg acatcgatgt
    32701 acttcgctat ttccttgttg acctcctgag ccttctcctt gccgcctatg ctcttccaaa
    32761 gggaggggcg gtagttaagg tcgtaggaga ctatggtgcc gtacttcttt gcggccttca
    32821 cagcctcgat aacaacctga gcggtggttt cggagagagc ggcgaaaatt ccgcctgtgt
    32881 gcatccagcg aacgcccagg gtgccgaaga tatagtccca gtcgatgtcg ccgggtttca
    32941 gttgagaagc ggcggtgttg cccctgacgg aaacgccgac c
//
LOCUS       DAONVQ010000005        32426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_885_length_32426_cov_4.950295, whole genome shotgun sequence.
ACCESSION   DAONVQ010000005 DAONVQ010000000
VERSION     DAONVQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 32426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32426
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_885_length_32426_cov_4.950295"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PLD98_00860"
     CDS             <1..787
                     /locus_tag="PLD98_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQA04839.1"
                     /translation="VAVAFAYEGWIIATSINAELKDAKKTLPKALVIGALIVVAVYIF
                     YYIGLAGAVSTEEIMADGHAGAANAFKNVFGTALGSLIFVFVIVSCLGTLNGLMVGTT
                     RAAFALASRNEGPRPVTFKQVDQITDMPSNSSVLGLFMCALWLLYFYGCTLMGWIGDF
                     GFDSSELPIVFLYTCYIPMFIAVMIKEKDFSPFKRFVVPLAAIISCGFMTYAGFIHPV
                     ISASGDREPWKGILLFFLVLLFVIASGALIKFKGEKRLAAESK"
     gene            complement(824..1822)
                     /locus_tag="PLD98_00865"
     CDS             complement(824..1822)
                     /locus_tag="PLD98_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQA04840.1"
                     /translation="MTTTTTSGDEKLTTEKDDATTSATWTEGETEWTSPDGAPVPDSS
                     TEPIVAEVVELPSGIKKNTVSLYGEYTKTEATYTGLEPLPKAEFKVHDPDNSRNLPTK
                     LIAHAYGVAKDGKPNDISVRSQAFFDSKNYNAITYDNKTDKKVLYLTFDVGYENGYTA
                     KILDVLKEKNVPAMFFCTLPQIKQYPQLIARMILEGHVVGNHSKTHPSFAKISRTKMM
                     EEVKAFDDYIREKFGYTAPFFRYPMGEYNESSLELLNSFGFKCVFWSVAYADWDLNNQ
                     KGKEHAFNTVTSRLHPGAVILLHSVSPDNANALGDIIDYARQQGYEFKLLPNKRQK"
     gene            complement(2051..2236)
                     /locus_tag="PLD98_00870"
     CDS             complement(2051..2236)
                     /locus_tag="PLD98_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04841.1"
                     /translation="MTKKTTNLMKGVGAALAIGGTAAVIGSMMNSTRNTRKAKKTATK
                     AVKTVENIIDSVQDIIG"
     gene            2413..4107
                     /locus_tag="PLD98_00875"
     CDS             2413..4107
                     /locus_tag="PLD98_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HQA04842.1"
                     /translation="MKIKLFFKRVFNLFLTFLFFIGSGGLKPTPNLDDPTLKAPQWPK
                     WVHEHWVWENVGTEDTAREFVNGFLERGIPVGAVIIDRPWATDSNTFIPDPNLYPNLD
                     KLIEEFHSKDIRVIMWATSIVNETASNFKEGKEKGYFLSGGRTVKWWGGKGALLDYSN
                     PEAVEWWHKQMDPILDMGIDGWKVDGTDPYVMLLAPAIGKSGLVTWNTYKDWVYRDFY
                     EYTRQRLGNDRIISARPVDDQITNLGLPLVSTSRDINFAGWVGDNDNDWGGLRHALNN
                     MFSSAQFGFVSYGSDIGGFRNDGNMYKDVFIRWAQLGAFCPIMENGGGGEHRPWMFDE
                     ETNEIYRKFVKLHHELIPYIYSQAAYSYETNRPTMRPLKGEYMYMLGDEILVAPYFDE
                     GETRLVYFPEGEWIYMFDESRHYKKGVEAIKFPLDEFPVFIRKGAIIPLDVADSENGH
                     GSELSAPFTTVMLYPEEGTNKFGLYEENKTGAMLSYTKKGSQLTISATESERSFLFRV
                     YGEKAPKQVKLGDTAIPAASSFEELTATDSGYFYDGSLLWISLKDASHGAEIKAYF"
     gene            4186..6510
                     /locus_tag="PLD98_00880"
     CDS             4186..6510
                     /locus_tag="PLD98_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA04843.1"
                     /translation="MKHFFNALPERAHFLYNKKDGDFKVKIASRGNSLLSVKLFEPIS
                     GETKTLNEGKEYIHGEDEIIFKADFMKTLPEGVYSIFTARYSRGSAVNIPVYISAGEL
                     PKSAVLFDPSDYKGDRLKEISLPRVTRAAIEEVTDENLPFENCLSLSYDLRRFVGLRK
                     PAARLKKTPLWHLPAFKWVSVWLCGDGSGNIIRFYINSRDNTPSHSKNEYVINWHGWK
                     RLCFNINDEFSLPFMFHQLFEFDYTLEGAQQGTIKVGKIESLYDESLCPSDMGRPVNK
                     YEPPAEPFMLDYKKGEKEGETQFFLKYLNDKHRKERAFLVLDSCRDNEGRKIEIPFEP
                     LAFDKNGVLKTDFFAAFPSDYAFWLRAETESGRSTTLFPFNNSVDTPLYSELPPLPVR
                     TVTKDNSRSAAFCWLSDDEKAESFMEIVRPDGRAERINAEKSYTREWAESKTIAGQSF
                     KFFKNICSNKATAENLLPDTEYKARFYSGDKVSEHTFKTLPEPENTEKFSLVFLADSQ
                     LSKKEDLPFLRKTFDEALNNSDKPRLIVSLGDMVDNAGSAREFQYILTHLSPFAAEEI
                     WLPTPGNHDQDDLKDLDFFLSRYNLPDRADGAKDGLCFHLTMGDYALISALGSGGGVD
                     LSYKERLERELTAARDKKWKIVFIHASPYGKSGERFTKAHYAELFDKYEVDAVFSGHD
                     HIYIRSSVRNGQRAEIGKGTLYCVMGTCGVSFDRAAYLPWHDYVYGDFDDEVKDSEGN
                     TDQTYGVAWCESDKLTIKVFTRTGKVVDEIVLTK"
     gene            complement(6544..8826)
                     /locus_tag="PLD98_00885"
     CDS             complement(6544..8826)
                     /locus_tag="PLD98_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA04844.1"
                     /translation="MEKIKNFLKTQKQYWKVPPKGRYMPYKEIISYSSGGMGVRLIVF
                     LVQNMILGVGNTLIGNTIGIAPRPLYIIYIISVLSGFPLTALRARMIDNTRGKEGKYR
                     PYIIKMGIPTVILGIGFVWMPYERMSLALKCVTVLLFNIGFQFFYNFLSDAYYSLVFL
                     LSPNTIERSDVCAIRSIIENFTPSIVGIFLPIVASWITGDSTLYDIRIYRYIYPPLLF
                     GGFFLAIIAYTGTKERIVQAKTHIIQVRLIDALRAVWRNKYFWIFSLANWLGFLEGAF
                     DNILGWLYNYQKACSPAQYSAITAIIGNASFWNNVIAPGFIRKYGKRKILIVTNLMNI
                     FLIFLLYPIVKSFAPAVMIWMLTAVIFINRLITALGHLLHPSIEADIRDYQHYITGER
                     IDGMFAAVGLIGAAVSLATSFVLPAIYERVGLNERVAVSLGYSGDNVYDVLYNSEFFV
                     KIASVLILSAVVGATLNVIPFFFYDLTETKQRAMVTVLKIRALFEDYAAGELSDNSLI
                     EAIELIEEAKEFYGREPHKLTKDEIKAAKKSKDKAAVKQAKAKYKRKKEENEKIEIAE
                     YVIREINRFDTEEGQLEIETAKLIVEAGLKGFADLKLLSVEEAKAMPKGTAFEKEKRR
                     NAIIQAENIKFARKTIRKYYPDGLEEFDKSIFDRLQKEMEELEEKQKELIDLLSEARK
                     KKDKKSEKEIKADIKAVQLRKQAVNSAFKDASKKFSIYLTAAKPYINAQKLLLQKENY
                     SRYGEILERYEAAKKEKANA"
     gene            complement(8848..10212)
                     /locus_tag="PLD98_00890"
     CDS             complement(8848..10212)
                     /locus_tag="PLD98_00890"
                     /inference="COORDINATES: protein motif:HMM:NF025938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HQA04845.1"
                     /translation="MAVISVDLKKEYQSFEGFGASGAWWAQLVGGWEHSDPVSGMAVR
                     DRISQLLFSKDKGIALRTYRYNLGGGSKEAGNGKFPNPLRRAECFLNEKGEPDFTKDK
                     NAVYMMRKAVNDGAEELIFFVNSPPVCFTKNGKAHSDTAGRENLPKKNYRKFANYCLD
                     VAQHFISEGLPVKYLSPVNEPLWVWTGGQEGCHYRPRSVRKVFEVFAEELEKRPNLKG
                     LKLSGAENGDIRWFNKSYTRQLLKSPMVRKYLDSVDVHSYCLPAPFKFLNNRTAYLKR
                     FRKWMDKHYPEIPVKMSEWTHMKGGRDYGMDSALEMARVMYEDISILHVTSWQHWIAV
                     SEVDYCDGLIYINLEDKSFELTKRYFATGNFSKYVSHMAKRVEAKTDDPDILPLAFKG
                     ENTVVILINPTAEEKTVSINNLSGSARLIVTDDNKNLEETPLSSLENIKLTPRSVNTV
                     VLNE"
     gene            complement(10294..11595)
                     /locus_tag="PLD98_00895"
     CDS             complement(10294..11595)
                     /locus_tag="PLD98_00895"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HQA04846.1"
                     /translation="MSKDSPSPLATKRIPSIDRFRGLVIICMILFQFAEHFKNLGKWA
                     TVSLHAPNENAIYILPNLSLADLVAPAFILAIGLTFMPSMKRRIEKQGRKEAVSHFLR
                     RYLTLIGIGIAMNGINDILDGNDDLVSKIAIGLTALVLLFGVIALILKLAKVKNREKF
                     YKVYWVYMCIVGVFGIVVAIINAFMLVTGRTDESYGHWLVLHHIGFAGLVAIPFALLP
                     DKTGHYIRLLAGLGILSLYAVFHEGNLKNDLFPNNLELIDKVADGGFIGGFAWGAMLL
                     LYLFFAEEFRKRKNKYLPPASFGIFTIVFIGLLWSIFKTLPETDTWAGALSGFLPINK
                     GSVSPSYVLITTYLALLVFYIFELFNSLETKFDILAWWGKNPILMYCIEFGFVGALTA
                     ALGDYFETAPAPIGAAFVIGVGVLLTVVAYILDKKKIIIKL"
     gene            complement(11600..12478)
                     /locus_tag="PLD98_00900"
     CDS             complement(11600..12478)
                     /locus_tag="PLD98_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQA04847.1"
                     /translation="MGKYLIVNADDFGLCKSANDAVIDLFLDGKLLSSTIMFPAPGHE
                     QAARFAAEHPEYAIGVHLTLTSEWPTYRWKPLTDGKSLVDKDGYMWPKTEDVEKNATY
                     EDLEKEIRAQIDYALSFGFTPSHIDNHMGTLYGNRTGRFGLLKMILRILGELGYSYRL
                     YTKVDKSMVPRGTPYPVYKISSLFTSHWAKKFKVILPDYLLFPDWTRELREGTYEQYR
                     DLILKLWTNIPDGVTETFVHPAFATDEMKSITGSWRDRQWEYDLLKDPDTHKYLKDHG
                     VTMINYRDLIQMKKGR"
     gene            13332..15023
                     /locus_tag="PLD98_00905"
     CDS             13332..15023
                     /locus_tag="PLD98_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA04848.1"
                     /translation="MKKIFKSAVAVILCAALLCGTAVISFAGGDTLKIAVATDIHFSS
                     FSDLGEFPADPEAVPFTEGLLNKETYAYASIQGRMDWESKAIIKQLLKEFAESDDEFL
                     LIAGDLTSSGKRQSHLELAEILRNAEAESGKEIFVIGGNHDFEAKGSEGITIDEFKEI
                     YADFGYNEALSVHETSGSYTADLSGNYRLIAVDSCIYGEVGGEINHAVLSWIRKQAEK
                     AKLDGKKLIAMMHHPLLPHFAVQPLTNSGSYSQLLRELSDLGIDIVFTGHIHANDISS
                     AKTLSGKTIYDVMTGSLIVYPHAYRHAEFGGEKVKITSDYITEIDTADLTEGYTAEQL
                     QMIENNFPEYSQGFFEACMCRWLNKYVGSAEKVGGWLGLKKGTKAFNELNRLMNAIGK
                     AISLPIYDDGSGDDSIEKFALEAGFTLPESDYERPYQIVAAVMSNFYYGDEREMESET
                     ELVFLCLKAIVLHFGVTLASDAPVGSLLNFLGIRLDEDFSLSGEEFPKVVTEKSLQAL
                     LTALLGGITEDYSEPSDINAELDTSAKAPDISLISRIVNFLLSVFKAFSKLFINL"
     gene            15045..16544
                     /locus_tag="PLD98_00910"
     CDS             15045..16544
                     /locus_tag="PLD98_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA04849.1"
                     /translation="MKIKEKIYYYKNDFLSGTLPLKEQLGYAGGIFGNAMGQDAVLTF
                     SDKFNRNYMGISNKNLLIRGNVSTILSFIIPPAAGAWYDTSQSNIRKALRIMPIPFAV
                     TSMLLFVVPSTSALFNFIWVFFFGLFFSIVDTFYDIALNALALKLVTNPKDRKNFYTA
                     ESLAATLGSMLPGWLIPIVVGTTDDAKRQQMLYFFVALGFCIIGVLTMYAPYFKIDEK
                     RYLMMAAAAKSQQEEKIKWDLEKVSMILHNRPFMVLQAASAFEMIRKITYEALPYLYD
                     DVFGDYQMKAIIDIISGTFSYAGLFAVPFVGKKISARGMLMGGYAYTGILYIFMSLFN
                     LGFDLENLRKKRYVVGILIGFAGMPNAAQAAARKILVADSTDYMEWYAWKKYGDPMRC
                     DGMLVAASSIVGKALELIKANLYNGLFALVKYQTKDPASNVKPVQTVSTLRGLYAIIT
                     LCGLIGNLLAAFALLFDNYTGKRRDAIFKELTELRAARGAEARECDTVC"
     gene            16538..18115
                     /locus_tag="PLD98_00915"
     CDS             16538..18115
                     /locus_tag="PLD98_00915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQA04850.1"
                     /translation="MLTAIKTELPVAKSPEAAGVSSEKLIEMLSELKKIDCHGFMVIR
                     HGKIAARWFRYPYAADTPHAMYSVSKSITGIAIGFAVSEGLLSLDDKVADFFPEYVPK
                     NCDENLKKVTLKHLITLSAGRNVSPFKSKVRHDWIKLFMDSKCDYAPGEGFSYINENF
                     HMALAMLRKVTGVSAVEFLRPRLFEPLGIEVPYWETDHKGVEAGGWGLHLSPVDLAKI
                     AMCFLYDGKYEGKQIIPKEWVNEAVKNTKVKKQTGYNFGYGCGVWIRESEDGPVRFDG
                     MFGQVAEIHKKYDAVTVTVGGDMNARNRNIMFKYFPDAFIDPIPNAEPSEEYLKAVSE
                     GDYIPPYSEYRSPIESKIDGRKIAFEKKLLLNMIDFPVSVIPAAVTYMSKDKAGNITD
                     VSFNFKEDSLAFSWHEGGQDYSIECGLDGNWRVSKAVIGGAPYTAYSAAKWESENTLL
                     VHIRPSESPCARNLKFTFNGNKVEMLPSSDPPSDAILDSLRGTVRDFLKSEKRADALL
                     TWAKNTLEPVHKGRFKK"
     gene            18129..18854
                     /locus_tag="PLD98_00920"
     CDS             18129..18854
                     /locus_tag="PLD98_00920"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HQA04851.1"
                     /translation="MKTLLYVLIAAELIFVLIYAMPNVRKSKSKLPVKTVCAVTYVLI
                     GIAAALASGGYFTSYAILLTIGAVFGAIGDYSLNMDPKSKKFIIGVGSFLIGHVFYTA
                     AFATALVKNSALQKKQIIMLTISAAAVSLLVVAAGVLLKVSPGVGLPPVILYNLAIST
                     MLVFAVSTAVNLIKNQGSGAYPAALMVSLGAVLFVVSDAVLAIIHFSKRKIKIPHAVN
                     SWTYFPAQVMLALSIYFIQSLMV"
     gene            complement(18896..19336)
                     /locus_tag="PLD98_00925"
     CDS             complement(18896..19336)
                     /locus_tag="PLD98_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HQA04852.1"
                     /translation="MKTAIVYQSYHHGNTKKLLDAIAEKYEVTLIDAEKQRDYDLSQY
                     DRIGFASGIAVGKFYSKVLKAMKEKLPEGKEVFFIFTCGVMKDRYTNAAKKIARKKNA
                     KILGEYGCLGYDTVGIFKLFGGFAKGHPTREEIEKAVEFYGSLK"
     gene            complement(19447..19531)
                     /locus_tag="PLD98_00930"
     tRNA            complement(19447..19531)
                     /locus_tag="PLD98_00930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19495..19497),aa:Leu,seq:tag)
     gene            complement(19773..21044)
                     /gene="serS"
                     /locus_tag="PLD98_00935"
     CDS             complement(19773..21044)
                     /gene="serS"
                     /locus_tag="PLD98_00935"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQA04853.1"
                     /translation="MLDIRRIRENPEAVKAAMKSRNKDMDALIDSVLEIDRRRRELTV
                     KIDTIRSEQNAASKEIPRIKKEGGDVSAIMERMNALKEQAKEIDAELSELEDKQRAVL
                     LEIPNVPHESVPKGASDEDNVEIRRWGEPRKFDFEPKPHWELGESLDILDPETAAKVT
                     GARFHFYKGLGARLERAIVNFFLDTHTKHGYTEVFPPYMVNRASMTGTGQLPKFEEDA
                     FKVANDDYFLIPTAEVPVTNMFMNDILDGSQLPIKFCAYSACFRAEAGSAGRDTRGLI
                     RQHQFNKVELVKFADPEKSYEELESLTNDAERVLQLLGLPYRVVALSTGDLGFSSAKT
                     YDIEVWMPSYGRYVEISSCSNFEDFQARRAQIRFRRTPKDKPMLVHTLNGSGVAVGRT
                     TAAILENFQNADGSVTVPEVLVPYMGTDIIK"
     gene            complement(21099..21962)
                     /locus_tag="PLD98_00940"
     CDS             complement(21099..21962)
                     /locus_tag="PLD98_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQA04854.1"
                     /translation="MAAKKGGLGKGLDALFSSNSAEELSSSGKVTLKITDIEPNRNQP
                     RKVFDEAALNELAESIAQNGILQPLLVRPMADGSYQIVAGERRWRAARKAGLTEVPVY
                     IRALSDEEVAAMTLVENLQRQDLNPIEEAKGLKQLISDYGYTQEQAAEKIGKSRSAVA
                     NTLRLLSLPDEVLQLVSDGELSAGHARALLSLENPELIKETAKEVISKGLSVRETEKL
                     VKKLTKQRPIAEKSLPKRAPYFDEVELSLSETTGRKIKVRGDEKGGTLEIQFFDTEDL
                     TKLASLFDEWQ"
     gene            complement(21975..22739)
                     /locus_tag="PLD98_00945"
     CDS             complement(21975..22739)
                     /locus_tag="PLD98_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA04855.1"
                     /translation="MAKIISIVNQKGGVGKTTTAVNLSAAVGQMGYRVLLADIDPQGN
                     STSGFGVNKREISLSSYEIITSQAAIEDAILKTDCENVYILPSNMNLAGAEIEIIEVE
                     KRESILKTALLKVSDKFDYIFIDCPPSLGLLTLNALCASDTFLVPIQCEYYALEGLSQ
                     LMSTVRQVKRRYNPYLELESVLLTMYDGRLNLTQQVVAEVKKFFPNKVFKTVIPRNVR
                     ISESPSFGQPVIYYDKRSQGAESYMRLAEEFLEKQK"
     gene            23685..24953
                     /gene="dinB"
                     /locus_tag="PLD98_00950"
     CDS             23685..24953
                     /gene="dinB"
                     /locus_tag="PLD98_00950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQA04856.1"
                     /translation="MRAILHSDINNCYAGIEEIFHPELKGVPFVVGGDESKRHGIVLA
                     KNYPAKAYGIKTGEALFSARAKCPHLKVLPARMELYSEYCSAVRRIYLDYTDMVEPFG
                     MDECWLDVTGSVMLFGSAVKIAHEIKERVKKEIGVTVSIGVSFNKVFAKLGSDYKKPD
                     AVTVIDKDNFKEIVWPLDCGELLYVGRRTKEKLRDRGILTIGDLANTSPEKLKRFLGK
                     NGEMLYIYANGYDTTPVQKFDYSREIKSVGASTTTPRDLYTLGDVRAVVFSLSEEVGE
                     RLREKGLEGNLIALSVRDKYLNWCEKQRYLDRYTNLSSEIAAGAMGLFTDRFDLSVPL
                     RSIGVRIGNVKKESEGAVQLSLFCDETRREKRRRIELTTDKIKNRYGETSILRAVTMN
                     PLCRNNRSITALGFHSAKEDMSKLSESFKQ"
     gene            25093..25182
                     /locus_tag="PLD98_00955"
     tRNA            25093..25182
                     /locus_tag="PLD98_00955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25128..25130,aa:Ser,seq:tga)
     gene            26246..27547
                     /gene="dnaA"
                     /locus_tag="PLD98_00960"
     CDS             26246..27547
                     /gene="dnaA"
                     /locus_tag="PLD98_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQA04857.1"
                     /translation="MESLSDIWKQALEKCRGEVTEVSYNLWLAPLEFINFENSTFVLS
                     TSSAHKALIFNDKFKDLVKKNLSELMGFDVEVQVIVAAPIPAEPEEPQISVNDNEYTF
                     ENFIVGSSNKFAHAASYRVATAPGVEYNPLFIYGRSGLGKTHLMLAINHEIKKRRPNA
                     VILYTTGESFTNELITCIAEKNTKAFHNKYRNVDILLVDDIQFIQNKVSVQEEIFHTF
                     NTLTQNSKQVVLTSDRPPKEMELLDERLRTRFEWGLMADIQPPDIDTRMAIIVRKAAS
                     LGLTLSDSIVEFIAEKIKHNIRQLEGTVKKLHAINTLNGTPITMEVVQEVVKDVVTDN
                     EPVSVTVDKIIENVSNVYGVSVSDILSEKRNAAISTARQVSMYIIREITDLSQQQIGE
                     KFGGKSHSTVHHSISTVEDRIAKDIAFKNQVFDIIKNIQDK"
     gene            27855..28958
                     /gene="dnaN"
                     /locus_tag="PLD98_00965"
     CDS             27855..28958
                     /gene="dnaN"
                     /locus_tag="PLD98_00965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQA04858.1"
                     /translation="MKLVCNTSVLSQACQNVQRAASTKSVLPTIEGIFISAKEGFVEL
                     CGYDLEFGIRTKIEAEIEREGAVVINARTLCDILKHLPFDTVTIENDERNNCRIYGGE
                     TSYTISGISAAEYPEIPSVKSETSVSVKGESLRDMVRQTIFSVSTDDSKMVHRGIKFE
                     IEDNVMTLIALDGRRLALRRETVEYKGEKMSFVVPAKTMSEVTKLIDEETGDIEINLG
                     MRHIIFKVGGYSVVSRLLDGEFLDYRSALPKNKTTIVRIKTKDFADSIERASLVVTEK
                     FKSPLKFIFDDSSVKITGTTALGTASDRTDAVIEGSRVEIGFNYRYLTEALRACDTDE
                     VVIELSGPLSPICIVPTSGDSFLYLILPVKLNN"
     gene            29012..29281
                     /locus_tag="PLD98_00970"
     CDS             29012..29281
                     /locus_tag="PLD98_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HQA04859.1"
                     /translation="MEKKRKVIKIKAIPAERVEKELIITTEFIKLDSALKLAGEAETG
                     GHAKLMVSEGKVFVNGERCLQRGKKLFDGDSFRVGATVYKVVRLR"
     gene            29287..30423
                     /gene="recF"
                     /locus_tag="PLD98_00975"
     CDS             29287..30423
                     /gene="recF"
                     /locus_tag="PLD98_00975"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HQA04860.1"
                     /translation="MFVKTLKAENFRNLERIELEPSEGVNIIYGENAQGKTNLLELIW
                     LFTGFKSFRGAKDSELVRFGEDFSKTEAEFFDGRRVQKAQLVIVKKRKATLNGVEQSS
                     PSALSAEFSAVVFSPAHLSLIKDGPAERRKFLDLALCQLSRSYQSALYSYNKILSQRA
                     RLLKDIYMRPSLEDTLDVWDEKLAEFAVFLIDKRRKYVEILSEHSKAVYEGISSGKES
                     LKVSYSQGDFEINRKEDFVKRLRALRKEDILAGSTSVGPHRDDIKVEIDGMAARNFGS
                     QGQQRSAAIALKLAEAEIIKEISGKSPIILLDDVLSELDISRQDYILRCLGDRQTFIT
                     CCDPYSILRSCGGKTFYVENGTLTEKSRFDIEVKPPPQCEDREI"
     gene            30433..30696
                     /locus_tag="PLD98_00980"
     CDS             30433..30696
                     /locus_tag="PLD98_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HQA04861.1"
                     /translation="MFLHLGQSTVVTMDEIIGIFDLERTTVSKRTRDFLAHAEREGKV
                     INVSYELPRSFIITKREGEEEKIYISQLSSATLLKRSEQERIG"
     gene            30708..>32426
                     /locus_tag="PLD98_00985"
     CDS             30708..>32426
                     /locus_tag="PLD98_00985"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HQA04862.1"
                     /translation="MDNKLQNGTLDTQVNGEYDASHIQVLEGLEAVRKRPGMYIGSTG
                     PRGLHHLVYEIVDNSIDETLAGYCDHIEVQILPGDVISVKDNGRGIPVDINEQYGLPA
                     VTIVLTVLHAGGKFGNGVYKVSGGLHGVGSSVVNALSEWMEVEISQKGHVYFQRFEKG
                     DPVTELKIIGDTNETGTLIRFKPDPEIFTETTVFDFEVLQRRLREQAFLNAGLKITLS
                     DLRDENNIIEEVYCYEGGIASFVEFINESRGLTPIHPNAIYFSAASGDRCVEIALMYN
                     DGYNENILSFANNVRTVDGGTHETGFKTALTRVFNDYGRKYNILKENDRNLSGEDVRE
                     GLTAVISVKLTEAQFEGQTKSKLGNTDITQLVSQTVYEKLMIFFEENPSIARDIFGKA
                     LDAARAREAARKARDLARRKSALESNSLPGKLADCVDKNPENTELYIVEGDSAGGSAK
                     EGRDRMTQAILPLWGKMLNVEKARLDKVIGNDKLMPVVTALGTGIDEDFDITKLRYHK
                     IVIMADADVDGAHIRTLLLTFFFRYMRPLIEHGYVYIAQPPLYRISKGKKHAYAYSDE
                     ERDAIIE"
BASE COUNT         8155 a         7878 c         8080 g         8313 t
ORIGIN      
        1 agtcgctgtt gccttcgctt atgagggctg gattatcgcc acatccatca acgcggagct
       61 taaagacgcc aagaagaccc tgcccaaggc tcttgtaatc ggcgctctta tcgttgttgc
      121 cgtttacatc ttctactaca tcggccttgc aggcgctgta agcaccgagg agattatggc
      181 agacggacac gcaggcgcgg caaacgcctt caagaatgtg ttcggcacag ctctcggctc
      241 cctgattttc gtattcgtta tcgtttcctg cctgggaacc cttaacggtc ttatggtagg
      301 cacaacaaga gctgcattcg ctcttgcttc aagaaacgag ggtccccgtc ccgttacatt
      361 taagcaggtt gaccagatta cggacatgcc ctccaactca tccgttctcg gcctctttat
      421 gtgcgctctt tggctccttt acttctacgg ctgcacactc atgggctgga taggcgactt
      481 cggctttgac tcctcagagc tgccgatagt attcctttac acctgctaca tcccgatgtt
      541 catcgcggtt atgattaagg agaaggactt ctcacccttc aagagattcg tcgttcccct
      601 tgcggcaatc atctcctgcg gatttatgac atatgccgga tttattcatc ccgttatctc
      661 cgcctctggc gacagagagc cctggaaggg aattctcctg ttcttccttg ttctcttatt
      721 cgtcatagct tcaggcgcgc tgattaagtt caaaggcgaa aagagactgg ctgcagaatc
      781 caaataaacc ttaaataaaa agccttcgga aaaaccgaag gctttatttt tgtcttttat
      841 ttggcaggag cttgaattcg tagccctgct gccttgcgta gtcgattata tcgccgagag
      901 cgttggcgtt atcgggcgac acagagtgaa gaagtattac cgctcccggg tggagccttg
      961 atgttacggt gttgaaggca tgctccttgc ccttttgatt gttcaaatcc cagtcagcgt
     1021 aggcgaccga ccagaacacg catttaaagc cgaaggagtt taaaagctcc aaggagcttt
     1081 cgttatactc gcccatgggg tagcggaaga agggggcggt gtagccgaat ttctccctta
     1141 tatagtcgtc aaaggcttta acctcctcca tcatcttggt tcttgagatt ttcgcgaagg
     1201 aggggtgagt ctttgagtgg ttgccaacca catgaccctc aagtatcatt ctggcgataa
     1261 gctgggggta ctgcttaatc tggggcaggg tgcagaagaa catggccggc acattcttct
     1321 ctttcagcac atcaaggatt tttgcggtgt agccgttctc gtatcccaca tcaaaggtaa
     1381 gatacagaac cttcttatcc gttttgttgt cataggttat ggcgttgtag tttttggaat
     1441 cgaaaaacgc ctgagagcgg acggagatgt cgttgggctt tccgtccttc gcgacgccgt
     1501 acgcgtgggc tatgagctta gtgggaaggt tccttgagtt atcggggtcg tgtaccttaa
     1561 actcggcttt cggcaggggc tcgagtcccg tataggtcgc ctcggttttt gtgtactccc
     1621 cgtagagcga gacggtattt ttcttaatgc cgcttggcag ctcaaccact tccgccacta
     1681 tcggctctgt gctgctgtcg ggcacgggag cgccgtcggg gcttgtccac tcggtctcac
     1741 cctcggtcca tgtggctgag gttgtggcgt cgtccttttc agtcgtcagt ttttcgtcgc
     1801 ccgatgtggt ggtcgtggtc atcaaatccc cctgcttcat acaggagaac agggacagaa
     1861 taagacaaac acatagtata aagcacaata cttttttaaa gctcatttgc ggtttccttt
     1921 caagtgagat tttcactctt attatctcca aaaattcatt tgttataagc gttatgttaa
     1981 atttttcctc tgcccctcta ataaaaaata cacccgccct ttaggacagg tgtttttcaa
     2041 ggcaatttta ttatcctata atatcctgta cgctgtcaat aatattttcg acagttttta
     2101 cggccttagt ggcggtcttt tttgccttgc gggtatttct ggtgctgttc atcatggaac
     2161 cgattacggc agcagtaccg ccgatagcca gggccgcgcc gacacccttc atcaggttgg
     2221 ttgtcttctt ggtcattatc ttacctccaa taaagttttc atccttattt ttaacctcct
     2281 gtcaaaattt atttgaggta aaatttatgg atatcttata aaataatcca tctttgatta
     2341 aatttattga caatctgtta aaaataggac atactgaaaa tacattaaaa ggggtttttt
     2401 aggagtggac ttatgaaaat taagttgttt tttaaacgcg ttttcaattt gtttctgacc
     2461 tttttattct tcatcggctc aggcggctta aagcccaccc caaacttaga cgacccgacc
     2521 ctaaaggctc cccagtggcc caaatgggtg catgagcact gggtttggga gaatgtcggc
     2581 acggaggaca ccgcaaggga gttcgtaaac ggctttttgg agagaggcat tcccgtgggc
     2641 gccgttatca tagacagacc ctgggctacc gattccaaca cctttatacc cgacccgaat
     2701 ttatatccga atcttgacaa gcttattgag gagttccatt caaaggacat aagagtcata
     2761 atgtgggcga ccagcattgt gaacgaaacc gcctccaact ttaaagaggg caaggagaag
     2821 ggatatttcc tcagcggcgg cagaacggtc aaatggtggg gcggcaaggg cgctctgctt
     2881 gactattcca acccggaggc agtcgagtgg tggcacaagc agatggaccc cattctggac
     2941 atgggcattg acggctggaa ggttgacggc accgacccct atgtaatgct gctggctccc
     3001 gccatcggaa agagcggtct tgtaacatgg aatacataca aagactgggt ttacagagat
     3061 ttctacgaat acacaaggca gagactcgga aacgacagaa tcatatccgc cagacccgtt
     3121 gacgaccaga ttaccaatct tggactgccc cttgtttcca ccagcaggga catcaatttc
     3181 gcaggctggg tcggagacaa tgacaacgac tggggcggac tcagacacgc tcttaacaat
     3241 atgttctcct ccgctcagtt cggatttgta agctacggct ccgacatcgg cggattcaga
     3301 aacgacggca atatgtataa ggatgtgttc atccgctggg cgcagcttgg cgcattctgc
     3361 cccattatgg agaacggcgg cggcggtgag cacagaccct ggatgtttga cgaggagaca
     3421 aacgagattt accgcaagtt tgtaaagctc caccatgagc ttattcccta tatctacagc
     3481 caggccgcat attcatacga aacaaacaga cccacaatga gacccttaaa gggcgagtat
     3541 atgtatatgc tgggagacga aattctggta gctccctact ttgacgaggg agaaacacgc
     3601 cttgtgtact tcccagaggg cgaatggata tatatgtttg acgagtcaag gcactataag
     3661 aagggcgtag aggctattaa attccccctt gacgagttcc cggtatttat aagaaagggc
     3721 gcgattattc cgctggatgt tgcagactcc gagaacggac acggctcaga gctttccgct
     3781 cccttcacca ccgtaatgct ctaccccgag gagggcacaa acaagttcgg tctttatgag
     3841 gagaacaaga cgggcgccat gctctcctac actaagaagg gcagccagct tacaatcagc
     3901 gcaaccgagt ccgaacgctc attccttttc agagtctacg gcgagaaggc tccgaagcag
     3961 gttaaactcg gcgacacagc tatccccgcg gcgtcttcct ttgaggagct gaccgctacg
     4021 gacagcggct atttctatga cggaagcctt ctttggatta gccttaaaga cgcctcacac
     4081 ggcgctgaga taaaagcata cttctaaaaa cagggggctt aaagccccct ttaatttttt
     4141 cctgttattt gctataatcg tataaaacta tttaaggaac ggtatatgaa gcattttttt
     4201 aacgcgctgc ccgaaagggc gcattttctt tacaacaaaa aagacggcga ttttaaggtg
     4261 aaaatagcct caagaggcaa cagccttttg agtgtaaagc tttttgagcc gatttcgggt
     4321 gagacaaaga ccctcaatga gggcaaggag tacatacacg gcgaggatga aatcattttc
     4381 aaggcggatt tcatgaaaac tctgcccgag ggagtttaca gcattttcac cgcaagatac
     4441 tcaaggggtt ccgctgtgaa tattcccgtt tacatctctg cgggcgaatt accgaaatcc
     4501 gctgttttgt ttgacccgtc ggactataaa ggagacaggc taaaggagat ttctctgccg
     4561 agggttacaa gggcggcaat cgaagaagtt acggacgaaa atctgccctt tgaaaactgc
     4621 cttagtcttt catatgattt aaggcggttt gtggggctta gaaaacctgc ggcaaggctt
     4681 aaaaaaacgc ctttatggca tctgcccgcc tttaaatggg tttctgtttg gctctgcggc
     4741 gacgggtcgg ggaatataat aaggttctac ataaactcaa gggacaacac cccttcccat
     4801 tcaaaaaatg agtatgtgat taactggcat ggctggaaaa ggctttgctt taatattaac
     4861 gacgagtttt ccctgccctt tatgttccat cagctttttg agtttgacta tacattagag
     4921 ggcgcccagc agggcacgat taaggtgggc aagattgagt ctctttatga cgagagcctt
     4981 tgcccttccg acatgggcag acccgtcaac aaatacgagc cgcccgccga gccctttatg
     5041 cttgattaca aaaagggcga aaaagagggg gaaactcagt ttttccttaa atatctaaat
     5101 gataagcaca gaaaagagcg agccttcctt gtgcttgact cctgtcggga caatgaggga
     5161 aggaaaattg aaataccttt tgagccttta gcctttgata aaaacggcgt tttaaaaaca
     5221 gactttttcg ctgctttccc ttcagactat gccttttggc tgagagcgga aaccgaatcg
     5281 ggcaggtcca caaccctttt cccctttaac aattctgtgg atacccccct ttattccgag
     5341 cttcctcccc tgcccgtcag aaccgtcacg aaggacaact cccgctccgc cgccttctgc
     5401 tggctcagcg acgatgaaaa ggcggagagc tttatggaga tagtccgtcc cgacggcaga
     5461 gcggagagga taaacgccga gaaaagctat acaagagagt gggctgagag caaaaccatc
     5521 gcaggccaaa gttttaagtt tttcaagaat atctgctcaa acaaggcgac ggctgagaat
     5581 cttttgcccg acaccgagta caaagcaagg ttttattcgg gcgataaagt aagcgagcat
     5641 acctttaaaa ccctgcccga gcccgaaaat acagagaagt tcagtcttgt tttccttgcc
     5701 gactctcagc tttctaaaaa agaggatttg cctttcctca gaaagacctt tgacgaggcg
     5761 cttaacaact cggacaagcc cagacttatc gtgagccttg gcgatatggt ggacaacgcg
     5821 ggcagcgcgc gggaatttca atatatttta actcaccttt cgccctttgc ggctgaggag
     5881 atttggcttc ccacccccgg caatcacgac caggacgatt taaaggattt ggatttcttc
     5941 ctctcccgct acaatctccc ggacagagcc gacggagcaa aggacgggct gtgctttcac
     6001 cttactatgg gcgactatgc cctgatttcc gcccttggca gcggcggcgg cgtggattta
     6061 agctacaaag agcgccttga aagagagctg acggctgcgc gggacaagaa gtggaaaatc
     6121 gtctttatcc acgcctcgcc ctacggcaaa tcgggcgaga ggtttacaaa ggcgcactat
     6181 gccgagcttt ttgacaaata cgaggttgac gccgtatttt cgggtcacga ccatatttat
     6241 atccgctcca gtgtcagaaa cggacagcgg gctgaaatcg gcaagggcac cctctactgc
     6301 gtcatgggca cttgcggggt atcctttgac agagccgcgt atctgccttg gcatgactat
     6361 gtttacggcg attttgacga tgaggtcaaa gacagcgagg gaaataccga ccagacctac
     6421 ggcgttgcat ggtgcgaaag cgataagctc acaataaagg tatttacccg aacgggcaag
     6481 gtggttgacg agattgtcct tacaaaataa agatgccgct atcttaaaga tagcggcttt
     6541 tttttacgcg tttgcctttt cctttttggc tgcttcgtat ctttcgagga tttcgccgta
     6601 gcggctgtag ttctctttct gcaaaagcag cttttgggcg tttatatagg gctttgcggc
     6661 tgtgaggtag atggaaaact ttttcgacgc gtccttgaaa gcggagttta ccgcctgctt
     6721 tcttaattgc acggctttaa tatcggcctt aatctccttc tcagactttt tatccttttt
     6781 cttccgagct tccgagagaa ggtctataag ctccttctgc ttttcttcca gctcttccat
     6841 ttctttttgg agtctgtcaa atatgctttt gtcaaactcc tcaagaccgt cggggtagta
     6901 ttttctgatg gtttttctcg cgaactttat gttctcggcc tgaattatgg cgttcctgcg
     6961 cttttccttc tcgaaagcgg tgcccttggg catggctttc gcctcctcaa cgctgaggag
     7021 tttcaaatcc gcaaagcctt taagacccgc ttccacaatg agcttcgcgg tttcgatttc
     7081 aagctgccct tcctctgtgt cgaacctgtt aatctctcga atcacatatt cggcaatctc
     7141 gattttttcg ttttcctctt ttttgcgctt gtatttagct tttgcctgct ttaccgcggc
     7201 tttgtctttt gattttttcg ccgctttgat ttcgtccttt gtgagtttat gaggctctct
     7261 gccgtaaaac tcttttgcct cttcaataag ctcaatcgct tcaatgaggg agttgtcgga
     7321 gagttctccc gcggcgtagt cctcaaaaag agcccttatt ttaagcaccg taaccatagc
     7381 ccgctgcttg gtttccgtca aatcatagaa gaagaaggga atgacattta aggtcgcgcc
     7441 aacgacggcg gagagaatca gcacagaggc aatctttacg aaaaactcgg agttgtaaag
     7501 cacatcgtaa acattatcgc cgctgtagcc caaagatacg gctacccttt cgttaagacc
     7561 caccctttcg taaatggcgg gcagcacaaa gctggtcgca agggacacgg cggcgccgat
     7621 aagacccacc gcggcaaaca ttccgtcaat tctctcgccg gttatatagt gctgatagtc
     7681 ccttatatcc gcctctatgc tggggtgcag aaggtgaccc agagcggtga taaggcggtt
     7741 tataaagatg acggcggtga gcatccatat catgacggcg ggcgcaaagg actttacaat
     7801 cgggtacaaa aggaaaatca ggaagatatt cattaaattc gtaactatca ggattttgcg
     7861 cttgccgtac tttcttataa agccgggggc tatgacattg ttccagaaag aggcgttgcc
     7921 gataatggcg gttatcgcgg aatactgggc gggtgagcaa gccttttgat agttgtaaag
     7981 ccagccgagg atattgtcaa aggctccttc aagaaagccc agccagttgg caagggagaa
     8041 aatccagaaa tacttgtttc tccaaacggc tctgagagcg tctattaagc gaacctgaat
     8101 aatatgggtc tttgcctgca cgattctctc tttagtgccc gtgtaagcga tgatagcgag
     8161 gaagaagccg ccgaacaaaa gcggcgggta gatgtatctg taaatcctta tgtcataaag
     8221 ggtgctgtcg cccgtaatcc atgaggcgac tatgggcagg aaaattccca cgatggaggg
     8281 ggtaaagttt tctataatgc ttctgatggc gcagacatcg gagcgttcaa tggtgttggg
     8341 ggagagaaga aatacaaggg agtagtaggc gtcgctaagg aagttataga aaaactgaaa
     8401 gcctatgtta aaaagcagca ccgtaacgca ctttaaagca aggctcattc tctcatacgg
     8461 catccagaca aagcctatgc ccaaaattac ggtggggatg cccattttta ttatgtatgg
     8521 acggtacttg ccctctttgc cccttgtgtt gtcaatcatt cttgctctga gagcagtcag
     8581 cgggaagccc gaaaggacgc tgattatgta gatgatataa aggggtcttg gggcaatgcc
     8641 tatggtgttg ccgataaggg tattgcccac gcccagtatc atgttctgga caaggaaaac
     8701 aataagtctt acgcccattc cgcccgatga gtaggaaata atctccttgt acggcatgta
     8761 tctgcctttg ggcgggactt tccagtactg cttttgagtt ttaaggaagt tctttatctt
     8821 ttccataggc tcaaaattct ctccctctta ttcgtttaaa acaacagtgt ttacggagcg
     8881 gggtgtgagc ttaatatttt caaggcttga aagcggggtt tcctccaagt tcttattgtc
     8941 gtcggttacg ataagtcttg cgctgccgct gagattattt atcgaaaccg ttttttcttc
     9001 cgctgtgggg ttaatcagaa taacaaccgt attctcacct ttaaaggcaa ggggcagaat
     9061 atcggggtcg tcggttttag cctcgactct ttttgccata tgggaaacat attttgaaaa
     9121 attgcccgtg gcaaagtagc gttttgtaag ctcaaagctt ttatcctcaa ggtttatgta
     9181 aataagaccg tcgcagtagt caacttccga gaccgcaatc cagtgctgcc atgaggtgac
     9241 atggagaatc gaaatatcct cgtacatgac cctcgccatc tccaaagccg agtccatgcc
     9301 gtagtcccgc ccgcccttca tgtgagtcca ttcgctcatc ttaacgggaa tttcggggta
     9361 atgcttatcc atccattttc tgaagcgttt aaggtacgcc gttcggttat tcaaaaactt
     9421 aaacggcgcg ggcaggcagt aggagtggac atcaacggag tcaaggtatt tgcgcaccat
     9481 gggagattta agaagctgcc ttgtgtagct tttgttgaac cagcgtatat ccccgttttc
     9541 cgctcccgat aatttcagtc ctttgagatt cggtctcttt tccaattctt ccgcaaagac
     9601 ctcaaaaact tttcggacgc ttctcgggcg gtagtggcag ccctcctgcc ctcccgtcca
     9661 gacccacaag ggctcgttta cgggggacaa gtattttacg ggcagaccct cggaaatgaa
     9721 atgctgtgcc acatcaaggc agtagttcgc gaactttctg tagtttttct ttggcaggtt
     9781 ctctctgccg gcggtgtcgg agtgggcctt tccgtttttc gtgaaacaaa cgggcggtga
     9841 gtttacaaaa aagataagct cctccgcgcc gtcattaacc gcttttcgca tcatatagac
     9901 ggcgtttttg tcttttgtaa aatccggctc gcccttttcg ttaaggaaac actcggcgcg
     9961 ccttaaagga ttggggaact tgccgtttcc cgcctctttt gagccgccgc cgagattata
    10021 gcggtaggtt cttaaagcta tgcctttgtc tttgctgaaa agaagctgag aaatacggtc
    10081 ccgaacagcc atgccgctta cagggtccga atgctcccag ccgccgacaa gctgcgccca
    10141 ccaggctccg ctggctccaa agccctcaaa gctctgatac tcctttttaa ggtctacgct
    10201 tatcaccgcc ataaaatcac cctttgccat attaccacaa agttgcagat aaaaaaaggc
    10261 gcagcatatt ttgccgcgcc tcattttttg catttagagt ttaataatta tcttcttctt
    10321 gtcaaggata taggcgacaa cggtcagcag cacgcccacg ccgattacaa aggcggctcc
    10381 tatcggagcg ggagcggtct cgaagtaatc tcccagagcc gcggtgagtg cgcccacaaa
    10441 gccgaactca atgcagtaca taagaatcgg gttcttgccc caccatgcga ggatgtcaaa
    10501 cttggtctca agggagttaa agagctcaaa gatgtagaac accaagagcg caaggtaggt
    10561 ggtgataagc acataggagg ggctgacgct gcccttgttt atgggaagga agcctgacag
    10621 agcgcctgcc catgtgtccg tttcggggag agtcttaaag atgctccaaa gaaggccgat
    10681 gaaaacgatt gtgaaaatgc cgaaggacgc gggcgggagg tacttgttct ttctctttct
    10741 gaactcctcc gcaaagaaga ggtacagaag gagcattgcg ccccaggcaa agcctcctat
    10801 aaagccgccg tccgccacct tgtctataag ctcaaggtta ttcgggaaca ggtcgttttt
    10861 aaggttgccc tcatggaata cggcgtaaag ggagaggatg ccgagtcccg caagcaggcg
    10921 gatataatga cccgtcttat cgggcagcag ggcgaagggg atagccacca gacccgcaaa
    10981 gcctatgtgg tgcagaacaa gccagtgtcc gtaggattca tctgttctgc ccgttaccag
    11041 cataaaggcg ttgatgatgg cgacaacaat gccgaacacg cccacaatgc acatatagac
    11101 ccagtagact ttgtagaact tttccctgtt tttgaccttt gccaacttga gtatcagggc
    11161 gatgacgccg aaaaggagca caagggcggt aagtccgatg gcgatttttg agacaaggtc
    11221 gtcgttaccg tcaaggatgt catttatgcc gttcatcgcg atgccgatgc ctattaaagt
    11281 caggtaacgc cttaaaaagt ggcttaccgc ttcctttctg ccctgctttt cgattctcct
    11341 tttcatagag ggcataaagg taagaccgat tgcgagtatg aaggcgggag ccacaaggtc
    11401 cgcaagggag aggttgggaa ggatgtagat tgcgttctcg ttcggagcgt ggagtgagac
    11461 cgttgcccat ttgcccagat tcttaaaatg ctcggcaaac tggaagagta tcatgcagat
    11521 gatgacaagt cctctgaaac ggtctatgga gggaattctt tttgttgcta atggcgacgg
    11581 tgagtctttg ctcatgttct taccttcctt tcttcatttg tataaggtca cggtagttta
    11641 tcatggtaac gccgtggtct ttgagatatt tatgcgtgtc ggggtccttt aagaggtcgt
    11701 actcccactg tctgtcccgc catgagccgg tgatggattt catttcatcg gtggcaaaag
    11761 cggggtgcac aaaggtttcg gtcactccgt cggggatatt ggtccagagc tttaatatta
    11821 aatctctgta ctgctcgtat gtgccttctc tcagctcccg agtccagtcg gggaaaagga
    11881 gatagtcggg caggatgacc ttgaactttt tagcccaatg ggatgtaaac aggctggaga
    11941 ttttatagac gggatagggt gtgccgcggg gcaccatgct cttgtcaacc tttgtgtaaa
    12001 ggcggtagga gtagcccagc tcgcccaaaa tcctcagaat catctttaaa agcccgaacc
    12061 ttcccgtgcg gttgccgtag agggtgccca tgtggttgtc tatgtgcgac ggggtaaagc
    12121 cgaaggagag ggcgtagtct atctgggctc ttatctcctt ctccaagtcc tcgtatgtag
    12181 cgtttttctc cacatcctcg gtctttggcc acatatagcc gtctttgtcc acaagggatt
    12241 tgccgtcggt caggggcttc catctgtaag tgggccattc gctggtaagc gtcagatgaa
    12301 cgccgattgc gtattcgggg tgctcagccg caaagcgggc cgcctgctca tgccccgggg
    12361 cggggaacat tattgttgag gaaaggagct tgccgtcaag gaacaaatct ataactgcgt
    12421 cgttggcgga tttgcacaga ccgaagtcgt cggcgttaac aatcaaatat ttgcccaatc
    12481 tatcaccttc ataaaattac aaaatattta ctcttattat aataatatac caatatttta
    12541 ttaacattca atgtaaattt tatgaagaac tgactaaaaa tagccgcatg gcaagtccaa
    12601 tttggtaaaa ccgagaccca aaaagttcat acggggcact gatttttagc ttctcggcgt
    12661 ccttttcttt aatttaccga gcttgccaaa gcggcgggaa tttgccttgt atttgccgaa
    12721 aatcccgcct gagcggggaa actcggcggc gggcggttcg tgggagagcc ggcagcgggg
    12781 cgaccccctt tgatggcggg cggcgggcgg ggacggcgga aagcccgctg tgtgggcgac
    12841 cgatccccct gtcggtggcg gggctcggcg gcgctcttta caacggtgtc acccatgcgg
    12901 gcggacactc ccctcgggcg gggtggattg ctcgggcggc gtgtgtcgct cctgtcggtg
    12961 gcggggacgg cggaaagccc gctgtggagg cgaccgctct cccctgtcgg tggcggggcg
    13021 gggcgtttgg gttgttcggc ggcttacctc tttggcaggg tgggatgggg tggtttccct
    13081 tcggcggggg gtttggggaa atacgcgcgg aaacttatgt aaactgcata aatttcagta
    13141 aagtatataa atttatcaca aacaaaaaac atattggaca taaatatatg tttatattta
    13201 ataaatgagt aaatctcccc ggcaaaaggc gaaaaactct gttcaacttc aaacttttgt
    13261 ctgccggggg ttattattga gccttgggat atctacccgc aaggctttga ttcgtttccg
    13321 gaggtgcaac aatgaaaaaa atcttcaaat ccgctgtggc ggtaatcctt tgcgccgcgc
    13381 tgctctgcgg cactgcggtc atttcctttg cgggcggcga cacccttaaa atcgccgtcg
    13441 ccactgatat tcatttctcc agtttctctg atttgggcga attccccgca gaccccgagg
    13501 ctgtgccttt taccgaggga ctgcttaaca aggaaacata cgcctacgcc tcgattcagg
    13561 gtcggatgga ctgggagtca aaggctataa ttaagcagct tttaaaggaa ttcgccgaga
    13621 gtgacgacga gttcctgctg atagcgggcg accttaccag ctcaggaaaa aggcagtccc
    13681 atctggagct tgccgagatt ctgagaaatg ccgaagccga gagcggcaaa gagattttcg
    13741 tcatcggcgg caaccatgac tttgaggcta aaggctcaga aggcatcacc atagacgagt
    13801 ttaaggagat ttacgccgat ttcggctaca atgaggctct ttccgtgcat gaaacatccg
    13861 gctcttacac tgcggacctc agcggcaatt accgccttat cgctgtcgac tcctgcattt
    13921 acggagaagt gggcggcgag attaatcacg cagttttaag ctggataaga aaacaggctg
    13981 agaaagcaaa acttgacggc aaaaaactca tcgccatgat gcatcacccg ctgctgcccc
    14041 atttcgccgt tcagcccctt acaaattcgg gcagctactc acagctcctt cgtgagcttt
    14101 cggatttagg aatagacatt gtatttacgg gtcacatcca tgccaacgac atctcctccg
    14161 caaagacgct ttcgggcaag acaatttacg atgtcatgac aggctcgctg attgtctatc
    14221 cccacgctta cagacacgct gagttcggcg gcgagaaggt aaaaatcaca tccgattaca
    14281 taaccgagat tgacaccgcc gatttaaccg agggctacac cgccgagcag ctgcagatga
    14341 tagaaaacaa cttccccgaa tactctcagg gcttctttga ggcgtgcatg tgccgctggc
    14401 taaacaagta tgtgggctcc gccgaaaagg ttggcggctg gctgggtctt aaaaagggca
    14461 caaaggcttt caatgagcta aaccgcctta tgaacgccat cggcaaggct ataagccttc
    14521 ccatctacga cgacggctcg ggcgatgaca gcattgaaaa attcgctttg gaggcgggct
    14581 ttaccctccc cgaaagcgac tatgaaagac cctatcagat tgtcgccgcc gttatgtcca
    14641 acttctacta tggcgacgaa agggagatgg aaagcgaaac cgagcttgta ttcctctgtc
    14701 ttaaggccat cgttttacac tttggagtaa cacttgcttc cgacgctccc gtcggcagcc
    14761 tgttaaactt cctcggaata agacttgatg aggacttctc gctttcaggc gaggagtttc
    14821 cgaaagtcgt taccgaaaaa tccttgcagg cacttctcac tgccctgctt ggcggaatta
    14881 cggaggacta ttccgagcct tccgatataa acgcggagtt ggacacttcc gctaaagctc
    14941 ccgatataag ccttataagc agaatcgtta acttcctcct cagcgttttc aaagcattct
    15001 caaagctttt tataaatcta taaatcttat aagtttgagt ttttatgaaa attaaagaga
    15061 agatttacta ctacaaaaat gactttctca gcggaacgct gccgctgaaa gagcagcttg
    15121 gctacgccgg cggcatcttc ggcaacgcca tgggtcagga cgctgtctta actttcagcg
    15181 acaaattcaa ccgcaactac atgggcatca gcaacaaaaa tctccttatc cgcggcaatg
    15241 tgtccacgat tctcagcttt ataatcccgc ccgccgcggg agcatggtac gacacaagcc
    15301 agagcaatat cagaaaagct cttaggataa tgcccatccc ctttgcggta acctccatgc
    15361 ttttgttcgt ggttccctcg acctccgcgc tttttaactt tatctgggtg ttcttctttg
    15421 gactgttctt ttccattgtg gacaccttct acgacatagc tctcaacgct ctcgctctga
    15481 agcttgtcac caaccccaag gacagaaaga atttctatac cgccgaatcc cttgccgcga
    15541 ctctcggctc aatgctgccg ggctggctca tccccattgt ggtgggcaca accgacgacg
    15601 cgaaaagaca gcagatgctt tacttcttcg tggctctggg attctgcatc atcggcgttc
    15661 tgaccatgta cgccccctac tttaagattg acgaaaagag atatctgatg atggctgcgg
    15721 cggcaaagag tcagcaggaa gaaaaaatca aatgggacct tgaaaaggtc agcatgattc
    15781 ttcacaaccg cccgtttatg gttctccaag ccgcgtccgc cttcgagatg ataagaaaga
    15841 ttacatacga agcactgccc tacctttatg acgatgtttt cggcgattat cagatgaagg
    15901 caatcataga cattataagc ggcacattct cctacgccgg tcttttcgcc gtaccctttg
    15961 tgggcaaaaa gatttccgcc cgcggcatgc ttatgggcgg ctacgcatat acgggaattc
    16021 tttatatctt catgtccctg ttcaatctcg gctttgacct tgaaaacctt cgcaaaaaaa
    16081 ggtatgtggt gggtattctc atcggctttg cgggtatgcc caacgccgca caggcagcgg
    16141 caagaaaaat ccttgtcgcc gactccaccg actatatgga gtggtacgca tggaaaaagt
    16201 acggcgaccc catgcgctgc gacggaatgc tggtcgcggc ttcaagcata gtaggcaagg
    16261 ctttggagct tataaaggca aacctctaca acggtctttt cgcccttgta aagtatcaga
    16321 ccaaggaccc cgcaagcaat gtaaagcccg ttcagactgt aagcaccctc agaggtcttt
    16381 acgcaataat caccctttgc ggtcttatag gcaatttgct tgccgcattt gccctgctgt
    16441 ttgacaacta tacaggcaag cggcgcgacg ctatctttaa agagctgact gaactgagag
    16501 ccgcccgcgg cgctgaggct cgggagtgtg atacggtatg ctgaccgcaa ttaaaacaga
    16561 actgcccgtg gctaaaagcc ccgaggcggc gggagtatcc tccgaaaagc tcattgaaat
    16621 gctctcggag cttaaaaaga ttgactgcca cggctttatg gtgataaggc acggcaaaat
    16681 cgccgcccgg tggttcagat acccttacgc cgccgatact ccccacgcca tgtactccgt
    16741 aagcaaaagc attacgggca tcgccattgg ctttgccgtc agcgagggac ttctctccct
    16801 tgacgacaag gtggcagact ttttccccga gtatgtcccc aaaaactgcg acgagaattt
    16861 aaaaaaggta actttaaagc accttataac tctcagcgcg ggcaggaatg tgtcgccctt
    16921 taaaagcaag gtcagacacg actggataaa gctctttatg gactccaaat gcgactacgc
    16981 ccccggcgag ggcttttcct acataaacga gaattttcat atggcccttg ccatgctgag
    17041 aaaggttacg ggcgtttccg ccgtagagtt tttaagaccc cgcctttttg agcctctcgg
    17101 aattgaggtt ccctactggg agaccgacca caagggcgtt gaggccggcg gctggggact
    17161 gcatctctcc cccgtggacc ttgccaaaat cgccatgtgc tttttgtacg acggcaaata
    17221 tgaaggaaaa cagattatcc ccaaagagtg ggtaaatgaa gccgtaaaaa acaccaaggt
    17281 taaaaagcag acgggctata acttcggcta cggctgcggc gtgtggataa gggagagcga
    17341 agacgggccg gtaaggtttg acggtatgtt cggtcaggtt gcggaaatac acaaaaagta
    17401 tgacgcggtg acggtcaccg tcggcggcga catgaacgca aggaacagga acatcatgtt
    17461 caagtacttc cccgacgctt ttattgaccc catcccgaac gccgagccga gcgaggaata
    17521 tttaaaggct gtttccgagg gggattacat cccgccctac agtgaataca gaagtcccat
    17581 agagtcaaaa atagatggca ggaagatagc ttttgaaaaa aagctccttc taaacatgat
    17641 tgactttccg gtcagcgtaa ttcccgccgc ggttacatat atgtcaaagg acaaggcggg
    17701 aaacatcacc gatgtgagct ttaatttcaa ggaggattcc cttgccttct catggcatga
    17761 gggcgggcag gattactcca tcgaatgcgg gcttgacggc aattggcgcg tttcaaaggc
    17821 ggttataggc ggcgcgccgt atactgctta ctccgccgcc aagtgggaga gcgaaaacac
    17881 acttctcgtt cacatccgcc cgtcggaaag cccctgcgct aggaatttga aatttacctt
    17941 caacggaaac aaggtcgaaa tgctgcccag ctccgacccg ccgtcggacg ctattcttga
    18001 cagcttaaga ggcactgtca gagatttctt aaagtctgaa aagagagccg acgcgctttt
    18061 aacctgggct aaaaacactc ttgagcccgt acataaggga cgattcaaaa aatgagagga
    18121 gagtcgcttt gaaaacactg ctttatgtac tcatagccgc tgaacttata tttgtgctga
    18181 tttacgccat gccgaatgtg cgtaaaagca aaagcaaact gcctgttaaa accgtctgcg
    18241 cggttaccta tgtgctaatc ggcattgccg ccgcgctggc gtcggggggc tattttacct
    18301 cctacgctat acttttaacc atcggcgcgg ttttcggcgc catcggggat tactccctta
    18361 acatggaccc gaaatccaaa aagttcatta taggagtagg cagctttctt ataggccatg
    18421 ttttctacac cgccgcgttc gccacggctt tggtcaaaaa ctccgcgctc cagaaaaagc
    18481 agattataat gctaacgatt tcagcggcgg cagtttccct gcttgttgtg gccgcgggag
    18541 tgctgctgaa ggtatcgccc ggcgtgggtc tgcctccggt aattctttat aatctcgcca
    18601 tcagcacaat gctggttttc gctgtgagca cggcggtaaa tctgataaaa aatcaaggca
    18661 gcggcgccta tcccgcggct ttgatggtgt ccctcggggc tgtattgttc gttgtctccg
    18721 acgcggtgct ggctataata cacttctcaa agagaaaaat caaaatccca cacgcggtca
    18781 actcctggac atacttcccc gcccaggtaa tgctcgcttt aagcatctac tttatacaaa
    18841 gccttatggt ataaaaaaag gacctgtttg gcaaaccaaa caggtcttta ttttgctatt
    18901 ttaaactgcc gtaaaactcg accgctttct caatttcttc cctagtcggg tgaccttttg
    18961 cgaaaccgcc gaataattta aaaattccga cggtgtcgta gcctaaacag ccgtattcgc
    19021 cgagtatctt cgcgttcttc tttctcgcga tcttctttgc ggcgttggtg tatctgtctt
    19081 tcatcacgcc gcaggtaaat atgaagaaca cttcttttcc ttcgggcagt ttttctttca
    19141 tggctttgag caccttggag taaaacttgc ccacggcgat tcccgaagca aagccgattc
    19201 tgtcatactg agacaaatcg tagtcccgct gtttttcggc gtctatcaat gtgacctcgt
    19261 atttttccgc tatggcgtcc aaaagtttct ttgtgttgcc gtgatggtaa gactgataaa
    19321 caatcgccgt tttcattttt ctcctccttt tattttctct gccaatttta gggtaacccc
    19381 aaattaaaag cctgtcaact ccgcaaaaga aaaaagccga cgctatttta agcatcggcg
    19441 cttatctggt gcgggtaaca ggggtcgaac ctgcacgcct attggcacaa gatcctaaat
    19501 cttgcacgtc tgccaattcc gccatacccg cgtatttgct ttaagagtta atgaggagta
    19561 tctgaaaacc gcccatacag ttcggattat cttgagcgtc atttgagttt ttaacgcgaa
    19621 gatatagaat tgaaattctt tattcgggga atcctttcgg actccccgaa tatattttag
    19681 tttgtttcgc tttttgggcg caatactcct gcagtattat agcagaaaga aagggcattt
    19741 tcaatcatca aatttattgc aaaatcttcc gcttatttta tgatgtcggt tcccatgtag
    19801 ggcacaagaa cctcgggaac ggtgacgctg ccgtcggcgt tctggaagtt ctcaaggatt
    19861 gccgcggttg ttctgcccac agcgacgccg gagccgttga gggtgtgaac aagcatcggc
    19921 ttatccttgg gagttcttct gaacctgatt tgcgccctgc gcgcctggaa gtcctcaaag
    19981 ttggagcagg aggaaatctc cacatacctg ccgtaagagg gcatccaaac ctctatgtcg
    20041 tatgtcttag ccgatgagaa gcccaaatcg cctgtgctga gggcaacaac tctgtatggc
    20101 agacccagaa gctgcagaac tcgctcggcg tcgttggtga gggactccag ctcctcgtag
    20161 gacttttcgg ggtcggcgaa ctttacaagc tcaaccttat tgaactggtg ctgcctgata
    20221 agaccccttg tgtccctgcc ggcgctgccc gcctccgctc tgaagcaggc ggaataagcg
    20281 cagaacttga tgggaagctg acttccgtca agtatgtcgt tcatgaacat atttgtaacg
    20341 ggaacctcgg ctgtggggat gaggaagtag tcgtcgttgg ctaccttaaa ggcgtcctcc
    20401 tcgaatttgg ggagctgacc cgtgccagtc attgatgccc tgtttaccat gtagggcggg
    20461 aaaacctcgg tgtagccgtg ctttgtgtgg gtgtcgagga agaagtttac aatggctctt
    20521 tcaagtcttg cgccgagacc cttgtagaag tggaaccttg cgcccgttac cttcgcggcg
    20581 gtttcggggt ccagaatgtc aaggctctcg cccagctccc agtggggctt gggctcaaaa
    20641 tcgaatttcc tcggctctcc ccagcggcgg atttccacat tgtcctcatc actggctccc
    20701 ttgggaacgc tctcatgggg cacattgggg atttcaagga ggactgccct ctgcttgtcc
    20761 tcaagctcgg agagctccgc gtcaatctcc ttagcctgct ctttaagggc gttcattctc
    20821 tccataattg cggagacatc gccgccctcc tttttgattc tgggaatctc cttgctggcc
    20881 gcgttctgct cgctgcgaat tgtgtcgatt tttacggtga gctcgcgcct tctccggtcg
    20941 atttcaagaa cactgtctat aagggcgtcc atgtccttgt ttctgctctt catcgccgcc
    21001 ttgacagcct cgggattttc tcttatgcgt ctgatatcaa gcatttatta ccattccttt
    21061 aaattagatt tcttttagta tatggaaatt tttatatttt attgccattc gtcaaagagt
    21121 gaggcgagtt tcgtcaaatc ctcggtgtcg aaaaactgga tttcgagtgt gccgcccttt
    21181 tcgtcgcccc ttacctttat ttttctgccg gttgtttctg agagagaaag ctcaacttcg
    21241 tcaaagtagg gagccctttt gggcagtgat ttttctgcaa tcggcctttg ttttgtaagc
    21301 tttttaacca gcttttccgt ttccctgacc gaaagaccct tggagattac ctctttagct
    21361 gtttctttta taagctcggg gttttcaagg ctgagcagag cccttgcgtg acccgccgac
    21421 agctcgccgt cgctgacaag ctgaagaacc tcatcgggaa gggacaaaag ccttagggta
    21481 tttgccacgg cggacctgga ttttccgatt ttctccgccg cctgctcctg agtatatccg
    21541 tagtcgctga ttagctgctt taagcccttt gcttcttcaa tggggtttag gtcctgcctt
    21601 tgaaggttct caacaagagt catggcagca acctcttcgt cgctgagggc tctgatatat
    21661 acgggaacct cggtaagtcc cgcctttctt gcggctctcc atcttctctc gcccgccact
    21721 atctgatagg agccgtccgc catgggccgc acaagcaggg gctggagaat gccgttttgc
    21781 gctatgctct ccgccagctc atttagagcc gcttcgtcaa aaacctttct cggctggttt
    21841 ctgtttggtt ctatatccgt aattttaagt gtcactttgc ccgacgagga tagctcctcg
    21901 gcggaattgc tgctgaaaag agcgtcaagg cccttgccga gaccgccttt ttttgccgcc
    21961 attttctcac tcctttattt ttgcttctcc aaaaactcct ctgccagcct catgtagctc
    22021 tcggcgccct gggagcgttt gtcgtagtag attaccggct gaccgaagct gggggactcg
    22081 gaaatcctga catttctcgg aatcactgtt ttgaaaacct tgttggggaa gaatttcttt
    22141 acttccgcca caacctgctg ggtcaggtta agtctgccgt cgtacatggt caagagcacc
    22201 gactccagct caagataggg gttgtacctt cttttaacct gccttaccgt tgacatgagc
    22261 tgggacagac cttcaagggc gtagtactcg cactgaatcg gaacgaggaa tgtgtccgac
    22321 gcgcacaggg cgttcagcgt caaaagtccc aaagagggcg ggcagtctat gaatatgtag
    22381 tcgaacttgt ccgagacctt cagcagagcg gttttgagta tggactcacg cttctcaacc
    22441 tcgataatct cgatttccgc ccccgcaagg ttcatgtttg agggcaggat atatacattc
    22501 tcgcagtcgg tttttaaaat ggcgtcctct atagctgcct gcgaggtgat gatttcatat
    22561 gaggaaaggc tgatttccct tttgttgact ccgaagccgc tggtggagtt gccctggggg
    22621 tctatgtccg caagaaggac acgatagccc atctgcccca cggcggcgga taaatttacg
    22681 gcggtagtgg tcttgcctac cccgcccttt tggttgacga tagaaattat tttagccata
    22741 cctaccccgt attttttgga aaatattgct ttataacgaa cttatggctt tattatatca
    22801 ctttgacccc aaaaatcaat gaacttagcc gaaaatttcg attctgtctc ctccgccccg
    22861 caaaagtcag cggggctttt aaaattaaaa tgtttcatgt gaaacaatct gcggctcccc
    22921 tgcccgaatg tttcatgtga aacattttat tttgccgcat attttccccg tacatgccca
    22981 tatatataat gaaaagtttt aataggggca tttcgctcgg gtcgttcccc ttgtcggggc
    23041 ggcgctcccc tctgcggctc ggcggggtgg ggtacgctcc tatcatctgt gtcggcgctt
    23101 tcctctgcgg ctcggcgggt ggcggctgcg gcgtgggtgg taattctctt gtgatttctt
    23161 gcggggtcgc taccctcctc gccggcgggt tgcctccccc tctgcgacgg aacgaaaggt
    23221 tccctctttg cgccggcggg tcatcggcgc tcgggcgaca ctcacctatg aaccgctggg
    23281 ctttcctcag cgggcggcgg gtttcttcca ctaacaagtc tctgcggcgg tgcgactatc
    23341 gactgcggaa ctatgaggtg gattcccttc accacagcgg cgggatgttg tatgcggggc
    23401 tatgggtggg tcggctcgca gctccccctc ggcgtggagc gcctcccctc ggcggtcgtc
    23461 tgagtttcct ctcggtgacg cctcccctct gccacggctc cctcttgcgg ctcaactgtc
    23521 ccattaataa gacttgtcga acattggaag aattttattg tttattttcg aacatttgtt
    23581 tggtatacta taaacgaaca aatgttctat taaaagcctc tgcggcgttt ttataaaaat
    23641 gtgttttaag gctcgtatca gggaagaatt taaagggtga tataatgagg gccatacttc
    23701 attccgatat aaacaactgc tacgccggga tagaggagat tttccatccc gaactaaagg
    23761 gtgttccctt cgttgtgggc ggggatgagt ccaaacggca cggcattgtg ctggctaaaa
    23821 actatcccgc gaaagcctac ggaatcaaaa caggggaggc gctgttttcc gcaagggcaa
    23881 aatgccccca tttaaaggtt ctgcccgcca gaatggagct ttacagcgag tactgcagcg
    23941 cggtgcggcg gatttacctt gactacaccg acatggtgga gccgttcggc atggacgagt
    24001 gctggctgga tgtcacgggc agcgtcatgc ttttcggcag cgcggtcaaa atcgctcacg
    24061 agataaaaga aagggtcaaa aaggagattg gcgtcaccgt ttcgataggc gtgtctttca
    24121 ataaggtttt cgcgaagcta ggcagcgact acaaaaagcc cgacgccgtt acggtgattg
    24181 acaaggacaa tttcaaggag attgtctggc cccttgactg cggcgagctt ttgtatgtgg
    24241 gcagaaggac aaaggaaaag ctgagggaca gaggaattct caccatcggc gaccttgcca
    24301 acacttctcc cgaaaaactg aaaaggtttc tcggcaaaaa cggggagatg ctttatatct
    24361 acgccaacgg ctacgacacc actcccgttc agaagttcga ctacagcagg gaaataaaga
    24421 gcgtgggcgc cagcaccacc acgcccagag acctttacac cctcggggat gtaagggcgg
    24481 tggtcttttc cctttccgag gaggtagggg agaggctcag agaaaagggt ctggagggca
    24541 atctcatagc ccttagcgta agagacaagt atttaaactg gtgcgaaaag caaaggtatc
    24601 ttgacagata cacgaatctc tcctccgaaa tagcagcggg agccatgggg ctttttacgg
    24661 acaggttcga cctcagcgta cctttaagga gcataggcgt tcggataggc aatgtaaaaa
    24721 aagagagcga gggcgcggtg cagctcagcc ttttctgcga tgagacaagg cgggagaaaa
    24781 ggagaaggat tgagcttaca acagacaaga taaaaaacag gtacggcgag acctccattt
    24841 taagggcagt caccatgaat cccctttgca gaaacaaccg cagcattacg gctctgggct
    24901 ttcactccgc taaggaggat atgtcaaagc tgtcggaaag ttttaaacaa tgaatttaaa
    24961 aatgttgaaa agttaaaggc agggcgggga agcgccgctc tgccttgaaa acggatataa
    25021 taaaagcgca ttggcaaatt tttagattga caaaagctcc gaaactgtgt taatatatgc
    25081 ttcgcaaaat aaggagagat accgaagtgg tcataacgga acggtcttga aaaccgtcgt
    25141 accccccggg taccgtgggt tcgaatccca ctctctccgc caccatatcc cgcaagcctt
    25201 gaaacaaaaa ggcttgcggg ttttgctttt tcgttatcca cgaaaaatcc acgaaaaata
    25261 cgaaaaaaat tatatttgaa tttggtttag taccttaatg gcgtattttt catcgcttgg
    25321 atacagatga gcataggtgt cgagcgtttg cttaatggta gagtgtccca accgccttga
    25381 gatttctaca atgtttactt tcttgtgaat tagataagaa gcgtgagagt gtctgaaatc
    25441 gtgaattcgg ttttttttat ccctgctttt tttgcatagt cggcattgcg tttttcaata
    25501 gttatgtctt tgagcggctt tattccaccg caaactctcc attcgttcga aaaacaatta
    25561 gcgtccttat agcgagcata atgttcatcg agaatctgct ttaactgttt tggtatttta
    25621 atatctctat ttgatttttt ggttttaggc gggtcttctc tgtcctcgcc tttaagtttc
    25681 tgagttattc ctctccttct ctctccgcca gtatccgcaa gttttaatac ttgcggattt
    25741 ttttattgcg cagagttttg tgcccgcgtt ttttttatga ggacgctgcg ggcggcgaat
    25801 gggttatgcg ggcgggggag gggtaaggca aaaaaaatag gcagcggtgc tgccgatacc
    25861 aaaacctttc gacaattatg tcgaaggctg tctgtttcta tttttggaag gaaagccgca
    25921 agatgagaaa acgagggcaa aaaaaacgaa ttctaaggaa ttaaaatctt ttgcaaacgg
    25981 gaaagaaaaa agctcaaatt tcaaaattta agcgtaaata ggccttattt tactttttgg
    26041 cccgaataaa tattttatac tctttaaaaa aacttgcaaa tttagtatat ttgtgctaaa
    26101 atattataag taaggcaaat tccaggtgca ggagtttctt aataaaaaac tttacctatt
    26161 acaataatat cacaaatctc ggcgacagca aagccccttc tttgctgtcg gtcggatttt
    26221 tgtcgggctt gggagggctt gatagatgga atctttgtct gatatatgga aacaggcgct
    26281 ggaaaagtgc aggggagagg taacggaagt ttcctacaat ctgtggctcg cgcctttgga
    26341 gtttataaat tttgagaatt caacctttgt gctcagcacc tcgtcggctc acaaggcctt
    26401 gatttttaat gacaagttca aggaccttgt aaagaaaaat ctctcggagc taatgggctt
    26461 tgatgttgag gtgcaggtaa tagtcgcggc tccgattccg gctgagccgg aagagcctca
    26521 aatttccgta aacgacaacg aatacacctt tgaaaacttt atagtaggct catccaacaa
    26581 attcgctcac gccgcttcat acagggtcgc caccgctccc ggcgtagagt ataatcctct
    26641 ttttatttac ggccgttcgg gactgggcaa gacccactta atgcttgcca ttaatcacga
    26701 aatcaaaaag agaaggccga acgctgttat cctctataca acgggcgaga gttttaccaa
    26761 tgagcttatt acctgcatag cggagaagaa cacaaaggcc ttccataaca aatacaggaa
    26821 tgtggacatt cttctggtag acgacattca gttcatacag aacaaggttt ctgtccagga
    26881 ggagattttc catacattta atactctgac tcagaacagc aagcaggtag ttcttacttc
    26941 cgaccgtccg ccgaaagaaa tggagctgct tgacgagagg ttgagaaccc gctttgagtg
    27001 gggacttatg gcggacattc agccgcctga catagacaca aggatggcta taatcgtcag
    27061 gaaagcggct tctttgggtc tgactctcag cgactccatt gtggagttca tagcggagaa
    27121 aatcaagcac aacataaggc agcttgaggg caccgtcaaa aagcttcacg ccatcaacac
    27181 ccttaacggc acgcccatca ccatggaagt ggtgcaggag gttgtcaagg atgtggtaac
    27241 cgacaacgag cccgtttcgg tcaccgtgga caagattatc gaaaatgtct ccaatgtcta
    27301 cggcgtgtcg gtaagcgaca ttctctcgga aaagagaaac gccgccatct ccaccgcaag
    27361 acaggtttca atgtatataa tcagagaaat tacagacctt tcacagcagc agataggcga
    27421 aaaattcggc ggcaagtccc actcaacggt tcaccactcc atcagcaccg ttgaggacag
    27481 aatcgcaaag gacatcgcct ttaaaaacca ggtcttcgat attataaaga atattcagga
    27541 taaataaggc tgtcggtttt aaacaatctt tagacaacgc accgttaaaa acagcttaaa
    27601 agttttgaac aagttttagg atttagacaa cgggttttga acattttcga aatctttcct
    27661 tttccgaaaa ttcttttaga cagccgctaa aaagttttga aaacctaaaa tttgcccgaa
    27721 agcgtgtctg cgcttgagtt tcggaagatt tcgaaacttt cgacagcccc tattactact
    27781 actaaaatat tatttaatta ttttatattg tcttatattg ttgccgctat gataacggaa
    27841 aggaatggtc ttaaatgaaa ttagtctgca acacaagcgt actgtcacag gcttgtcaga
    27901 atgtccagag agcagcgtcc acaaaatcgg ttctgcccac tattgagggc atctttataa
    27961 gcgccaagga aggctttgtg gagctttgcg gatatgacct ggagttcggc atcaggacca
    28021 aaatagaggc ggaaattgag cgtgaggggg cagtggtaat aaacgcccgc accctttgcg
    28081 atattttaaa gcatctgccc tttgacactg tcacaatcga gaatgacgag aggaacaact
    28141 gccgcatata cggcggtgaa accagctata ccatttcggg catttccgcc gcggaatatc
    28201 ccgagattcc ttccgtcaag tccgagacaa gcgtcagcgt caagggcgag tcacttcgcg
    28261 acatggtaag gcagaccatc ttttccgttt ccacagacga cagcaaaatg gttcacagag
    28321 gtattaagtt tgaaatagag gacaatgtaa tgacccttat agcccttgac ggcaggagac
    28381 tcgctttaag gcgggagacc gttgaataca agggcgagaa gatgagcttt gttgtgcccg
    28441 caaagaccat gtcggaggtt acaaagctca ttgacgagga aacgggcgat atagagataa
    28501 acctcggcat gaggcacatt atcttcaaag tcggcggata cagcgtggtt tcaaggctgc
    28561 ttgacggcga gttccttgac tacaggtccg ctctgcccaa gaataagacc acaatagtca
    28621 ggattaagac aaaggacttt gccgacagta tcgaaagagc gtcccttgtt gttacagaga
    28681 agtttaagag cccgcttaaa ttcatatttg acgattcctc ggtcaaaatc acaggcacca
    28741 cagccctcgg caccgccagc gacagaaccg acgccgttat agagggcagc agggtggaaa
    28801 tcggctttaa ctacaggtat cttacagagg ctttgagagc ctgcgacacg gacgaggtcg
    28861 taatagagct atccggaccc ctgtccccca tatgcattgt tcccacaagc ggcgacagct
    28921 tcctttatct catcctcccc gtaaagctca ataactaatc tcataaattt tggaaataat
    28981 ataaggatta gttttaggaa aagggaatgt tatggagaaa aaaagaaaag tcataaagat
    29041 taaggcaatt cccgctgaaa gagttgaaaa agagctgatt ataaccaccg agtttataaa
    29101 gctggactcg gcgcttaaac ttgcgggcga ggctgagacc ggcggtcacg cgaaactcat
    29161 ggtttccgaa ggcaaggtat ttgtaaacgg cgaaaggtgc cttcaaaggg gcaaaaagct
    29221 ctttgacggc gactccttca gagtaggcgc caccgtctac aaagtcgtgc ggctcaggta
    29281 agacttatgt ttgtaaagac attaaaggct gagaatttta gaaatttaga acgcattgaa
    29341 ttagagccgt cggagggcgt taacatcatc tacggcgaga atgcccaggg caaaacgaat
    29401 cttttggagc ttatctggct ctttacgggc tttaagagct tcagaggagc aaaagattcc
    29461 gagctggtgc gcttcggcga ggatttctca aaaacggaag cggagttttt cgacggcagg
    29521 agagtgcaga aggcgcagct tgtaatcgta aaaaagagaa aggcgacttt aaacggcgtg
    29581 gagcaaagct ccccgtcggc gctgtcggct gagttcagcg cggtcgtgtt ctcacccgcc
    29641 catctttccc ttataaaaga cggaccggcg gagagaagaa agttcctgga tttggcgctc
    29701 tgccagctca gccgctcata tcaaagcgcg ctttattcct acaacaagat tctgagccaa
    29761 agggcaaggc ttttaaagga catttacatg cgcccctccc ttgaggacac tctcgatgtc
    29821 tgggacgaaa agctggccga atttgcggtg tttttaatcg acaaaaggcg aaagtatgtc
    29881 gaaatcttaa gcgagcactc gaaagccgtt tacgagggaa tttcctccgg caaggaaagt
    29941 cttaaagttt cctactccca gggcgatttt gaaataaacc gcaaagagga ttttgtaaag
    30001 cggctcagag cgctcagaaa agaggatata ctcgccggca gcacaagcgt aggtccccac
    30061 cgcgacgaca taaaagtgga gattgacggc atggcggcaa ggaacttcgg ctcacagggt
    30121 cagcaaagaa gcgccgccat agccctgaaa ctcgcggaag cggagataat caaggaaatc
    30181 agcggcaaat ccccgattat actccttgac gatgttctga gcgaactgga tatatcacgg
    30241 caggattata tacttcgctg ccttggcgac aggcagacct ttataacctg ctgcgacccc
    30301 tattcaatac tcagaagctg cggcggcaag acattttatg tggaaaacgg cacactgact
    30361 gaaaaaagtc gttttgatat agaagtgaaa cccccgccac agtgcgaaga cagggaaata
    30421 taaggagaga ttatgtttct tcatctcggt cagtccaccg tagtgacgat ggacgaaatt
    30481 ataggcatat tcgaccttga aagaacaact gtctcaaagc gcacccgaga ttttctcgcg
    30541 cacgctgaga gagaaggcaa ggtaatcaat gtatcttacg agctgcctcg ctcctttata
    30601 atcacaaaaa gagagggcga ggaagagaaa atttatatat cacagctttc cagcgcaacg
    30661 ctcttaaagc gttccgagca agaaaggata ggatagaatt gtctgaactg gataacaaac
    30721 ttcaaaacgg caccttagac acacaggtaa acggcgagta tgacgcaagt catattcagg
    30781 tccttgaggg cctggaggct gtcagaaagc gtcccggcat gtatataggc tccaccggtc
    30841 ccaggggtct gcatcacctt gtctacgaga ttgtggacaa ctccattgac gagacccttg
    30901 caggctactg cgaccatata gaggttcaga ttctccccgg ggatgtcatc tccgtcaagg
    30961 acaacggacg aggcataccc gttgacatca acgagcagta cggactgccc gccgtcacca
    31021 tagttctgac ggttcttcac gccggcggca agttcggcaa cggagtttac aaggtttctg
    31081 gcggtctgca cggcgtcggc tcatcggtag ttaacgccct ctccgagtgg atggaggttg
    31141 agatttctca aaagggtcat gtttacttcc agcgttttga aaagggcgac cccgtcaccg
    31201 agcttaaaat catcggcgac acaaacgaga cgggaacatt aatcagattt aagcccgacc
    31261 ccgaaatctt taccgagacc acagtctttg atttcgaggt gctccagagg agactcagag
    31321 agcaggcgtt cctcaacgct gggcttaaaa tcaccctttc ggatttaagg gacgagaaca
    31381 acatcataga ggaagtctac tgctacgagg gcggcatcgc cagctttgtg gagtttataa
    31441 acgagagcag gggacttacc cccatccacc cgaacgccat ctatttcagc gccgcatcgg
    31501 gcgaccgctg cgtggaaatc gcccttatgt ataacgacgg atataacgag aatattctga
    31561 gctttgccaa caatgtcaga accgttgacg gcggtaccca cgaaacgggc tttaaaaccg
    31621 ccctcacaag ggttttcaac gactacggca ggaaatacaa catcctcaaa gagaatgaca
    31681 gaaatctctc cggcgaggat gtgagagagg gtctgactgc ggttataagc gtaaaactca
    31741 ccgaggctca gttcgagggt cagaccaaga gcaagctggg caatacggat attactcagc
    31801 ttgtgtccca gacggtttac gagaaactga tgatattctt tgaggaaaat ccctcaatag
    31861 caagggatat cttcggcaag gctctcgacg cggcgagggc aagggaggcc gcgagaaaag
    31921 caagagacct ggcaagacgc aaatccgccc ttgaaagcaa ctcgctgccg ggtaaactcg
    31981 ccgactgcgt tgacaagaac cccgaaaaca ccgagcttta catcgtagag ggtgactccg
    32041 cgggcggcag cgcaaaagag ggcagggaca gaatgaccca ggcaatcctc cccctctggg
    32101 gcaagatgct caatgtggaa aaggcaaggc ttgacaaggt cataggcaac gacaagctca
    32161 tgcccgtcgt gacagctctg ggaacgggca ttgacgagga ttttgacata acaaagcttc
    32221 gctatcacaa gatagtcatc atggcggacg ccgatgtgga cggcgctcac ataagaacgc
    32281 tgcttcttac cttcttcttc agatacatgc gcccgcttat tgagcacggc tatgtctata
    32341 tagctcagcc gcccctttac agaatctcca agggcaaaaa acacgcttac gcctactccg
    32401 atgaggagag ggacgccata atcgag
//
LOCUS       DAONVQ010000006        29680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_1026_length_29680_cov_4.815055, whole genome shotgun sequence.
ACCESSION   DAONVQ010000006 DAONVQ010000000
VERSION     DAONVQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 29680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29680
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1026_length_29680_cov_4.815055"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1458)
                     /gene="rlmD"
                     /locus_tag="PLD98_00990"
     CDS             complement(73..1458)
                     /gene="rlmD"
                     /locus_tag="PLD98_00990"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HQA04863.1"
                     /translation="MTAPLKKNDIIRLNIDGMTSEGSAVGRLDSFAVFVPNGAIGDVL
                     DCRIIKVKKNYAVGKIENIITPSPDRIESDCPVSDKCGGCAYRHISYEAELRIKRQTV
                     VDAFKRIGGIDIEVEPVIPADDVNFYRNKAQFPVREEKDEIRIGFFAPRSHRIIECGG
                     ICRLQPSEFGEIVNEVRQWMRENKIKAYDELTGKGLVRHIYLRKGFGSGEIMLCLVLN
                     GTLNEKDKDILIKRVSEKFPAVKSVYLNKNTKDTNVILGDEYSLVYGAEAIRDKLGEL
                     NVKLPPQVFFQVNKAQAERLYRKAAKLANLKEGETLVDLYCGTGTIGLFMIKESGLKN
                     VKLIGIEVVEESVETAKKNAVENGIENTEFICGSAETAAEILLQRNEQPDVLLLDPPR
                     KGCAPELIDIIAQINPKRIVYISCDPATLARDCKLFKEKGYTLSSCTPLDLFPRTPHV
                     ECVVLMSRVEK"
     gene            complement(1511..2350)
                     /locus_tag="PLD98_00995"
     CDS             complement(1511..2350)
                     /locus_tag="PLD98_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQA04864.1"
                     /translation="MKIKYLGTGASEGFPGLFCKCDVCERARKAGGKNLRTRAQALVD
                     NKLLLDFGPDTNMHAVTGGFDAHNINTCLITHGHSDHLYLEEMHIRKKGFAYADADKN
                     APATLHMYASPKTSDIIRGYDEYESLEECGALKLHEIKAFEKFEAEGFEITPLEAKHS
                     VVLDPLIYIIKGEKCLLYAHDTDYFTDATWEYLEKNKPYFDFVSLDCTGMLIDYPGSH
                     MGLKGNEEVKERLIKIGCADENTIFVINHFSHNGKLIHDELVPVAQEKGFIVAYDGLE
                     IEF"
     gene            complement(2378..3256)
                     /locus_tag="PLD98_01000"
     CDS             complement(2378..3256)
                     /locus_tag="PLD98_01000"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HQA04865.1"
                     /translation="MKKITKAIIPAAGLGTRVLPASKAVPKEMLNIVDKPAIQYLVEE
                     AAAAGITDILIITNRGKEVIENHFDYAFELEENLKANPAKKPLYDAVHNIADLANIYF
                     IRQKETKGLGHAILKAESFVGDEPFAVLYGDDVIISEEPVVGQLIKAYEKYGKSCVGV
                     NECTREQIKKYCTLDVTPLEGNVMDVHKIIEKPEEHEIMSLYAILGRNVLEPEIFDLI
                     RNTPPNPKSGEVYLTDALSYLAEHGRLTAVDFVGKRYDMGDKLGIMQANCEVALKHPE
                     IGESFKEYLKELAKTL"
     gene            complement(3297..4295)
                     /locus_tag="PLD98_01005"
     CDS             complement(3297..4295)
                     /locus_tag="PLD98_01005"
                     /inference="COORDINATES: protein motif:HMM:NF025802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HQA04866.1"
                     /translation="MWTNGDICPGCSKPLNKDEDIVVCPVCGTPQHRECWRKNNACVN
                     EHLHDEGYVWTSSVKEEKRKETPNSEPSFTAGERNDARRESADPYGEEPRSPFERPSF
                     AYATAIPPEALIDGIPAIELAQVVQTKVPRYLSRFFRIDQTNVKIGWNWGVCILALLF
                     SIFWNPISLFFPALWFFYRKMPKIGALVLAGALFFSVANISPSDARLYYEIKNASEQV
                     MENSGIISVEQFQEIIDKVIQKSGYTSEELKRKNVLNILAHAAIFGLASFGDYLYLGD
                     IKKRINKARKTATEPAQYQRLLYEMGGTSLGSALIAAVVYFFIDTAVKLLVISILA"
     gene            complement(4319..5656)
                     /locus_tag="PLD98_01010"
     CDS             complement(4319..5656)
                     /locus_tag="PLD98_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQA04867.1"
                     /translation="MNKFHKLICIILCLAAVFALPLTALADEEETTNYFDYYGSDLDK
                     EDEREKDKNAFDAASEFSNPNGRLLVVSNKGDWRNFPENSLEAVLSAFEKGADIVKVN
                     VKQLKDGNFVLMADDTTGRTCYGGDDKPLKELTAEEIKKYRLLSGVGEKMSRQTNFEV
                     PFLYDVLGAVQGKGMLMLDFDWALRDVLYNVVKDRDMLGDVIFYTEAKAKDIGEWASG
                     LEVKPHIMSGYKSNIVFMIGSYIKKSFEGGAEAVRLATKNPYGVIFDNFTMKKFESVG
                     RAAIDLTDPNLCGSREDTEKWWNDVVSRGYSIIITDYPEELSEYAKETEYAKTQLREA
                     YKRYKEQWVLPEFKSYTFGDYKRAYLWAVSDAEEYLSGRVRSKYDYLTARAALERAAV
                     NIEKNFTALQEGVAGLTITPPRILVAALSIIAVVSVQIYFHKKKKTEEKSVEC"
     gene            complement(5783..7546)
                     /locus_tag="PLD98_01015"
     CDS             complement(5783..7546)
                     /locus_tag="PLD98_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HQA04868.1"
                     /translation="MRHILEIRGRDFYLDGKKTIIRSGAIHYFRVLPEYWENRLLKLK
                     AAGLNTVETYVPWNLHEPKKGEFNFDGILDIVRFVKTAEKIGLNVIVRPGPYICAEWD
                     FGGFPAWLLKDENIRLRCGDKKYLKAVTDYFNVLLPMLAPLQKTKDGPIIAMQIENEY
                     GSFGNDKNYLSYLRGLMRSNGIDVLLFTSDGDNRYYLSGGGLEGELMVTNFNSMPADS
                     FKDLRELQPDKPLMCGEYWCGWFEAWGGKHPKGSAEKLGEAIRQFFDLNASFNLYMFH
                     GGTNFGFTSGANYYDKYTPTVTSYDYDAPLNEYGGYTDKYFAVREAIIEKTGESLPEP
                     PAPPVLQRIGKVNLTETASLLNNIKRLGKKHRSVSPRSMEYYGQNHGLILYSTEIEGN
                     YNDDKITIEGIHDKAFVWINGEFKGSFCRTDKTGRNEYSECFIVHFPAFSGKMRLDIL
                     VEAMGRVNYGNFIYDRKGIKNAKLGNQNLSDWLIYTLPLEDLSQIKFGNKDTQYPLFL
                     RGSFKAETNADCFVDMKGFTKGIVWVNGFNLGRYWKIGPQRTLYLPGALLKEENEIII
                     LELESSAKRSVEITDKPIYIK"
     gene            complement(7639..8034)
                     /locus_tag="PLD98_01020"
     CDS             complement(7639..8034)
                     /locus_tag="PLD98_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04869.1"
                     /translation="MIKRLVPLIIIFSLLLSLAACKESGGREADTTRRNASELENIEF
                     KAVVKEKYSGSILVKVIESPVDFSDLAYVLSEKASVKKDGEKIDFDGIKIGDELIIKH
                     HGYIAESYPVQLVGEKEIIVLSASTDTTA"
     gene            complement(8147..8221)
                     /locus_tag="PLD98_01025"
     tRNA            complement(8147..8221)
                     /locus_tag="PLD98_01025"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8187..8189),aa:Thr,seq:cgt)
     gene            8685..11411
                     /gene="secA"
                     /locus_tag="PLD98_01030"
     CDS             8685..11411
                     /gene="secA"
                     /locus_tag="PLD98_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQA04870.1"
                     /translation="MSILKKIFGDYSQKELKRIKPLCDKILALESTYEKLTDAELRAK
                     TEEFKKRYQNGESLDALLPEAFAVCREASWRVLGMKHFPVQVLGGIILHQGRIAEMKT
                     GEGKTLVATLPAYLNAIAGEGVHIVTVNDYLARRDSEWMGKVFRFLGLTVGLVIPSIP
                     HDQKAAAYAADITYGTNNEFGFDYLRDNMVMYKEQRVQRGHNFAIVDEVDSILIDEAR
                     TPLIISGRADKSTDLYKIADNFVKGLKCVKVTELSSKKNAEDELEEEGDYIVDEKART
                     ATLTKSGVAKAEKFFSLENLMDAENLTIQHHINLAIKARGVMQRDVDYVVKDGQVLIV
                     DEFTGRIMIGRRYSEGLHQAIEAKEGVNVRHESRTLATITFQNYFRLYKKLSGMTGTA
                     MTERQEFEEIYNLDVVEIPTNKPMIRIDHPDVLFKTEEAKFNAAIDQIVACHEKGQPV
                     LVGTVSIEKSEKFSKALKRRGIKHEVLNAKNHEREAEIIAQAGRLGAVTIATNMAGRG
                     TDIILGGNCEYMAKAEMRKMGIPEELIAESTSFAETDDEEILKARQIFKELEAKFKEQ
                     QAAEAEAVRKAGGLFIIGTERHESRRIDNQLRGRAGRQGDPGESRFYLSAEDDLLRLF
                     AGDRFYRTLDMFKSAGEMPIDAKIFSSAIESAQKKVEGNNFAIRRNLLKYDDVMNQQR
                     EIIYKQRNMVLDGVDLDATIQKMIEESIRSAVDFYCAKNIPSEDWNLDGLRSKYAWLL
                     KNKEDVNSDMSHDEVYETLLNLAMERFNHQKALYGEELMRALEHKILLENVDIRWMEH
                     IDAMEQLKTGIGLRAYAQKDPVVAFRQESFDMFEEMTENIRDGTVQMLLTAVVRTEKD
                     TERQQQAEITSQSGGDGSDKQRPVKKKESEKVGRNDPCPCGSGLKYKKCCGR"
     gene            11453..12517
                     /locus_tag="PLD98_01035"
     CDS             11453..12517
                     /locus_tag="PLD98_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HQA04871.1"
                     /translation="MNNINPGGFGFNPPPHYGAQPVGVPLVPPQNPPLPRRSRFDLRR
                     LSMLTGLSIIAFLIMSYAFSGIIVLFADDLKAYLKGISMDTSIFSYILESLLSVFGIG
                     LPFYIAYRAMRKKYGVDFIPFSPPESYTLMLLMIGAAFAACLLGSSFTSFLTIFLEEN
                     FGITSTYTMGDMPVTFDGKVLYFVRSAILPAFIEEFALRGVVMQPLRRYGNSFAIIVS
                     SIIFAIMHGNLIQAPFAFIAGIALGYAVIITNSIWTGVVVHLLNNSLSVILMFLTKGN
                     TEAQVPFYVAAIMPTIIFIGLVCFVIYLFSKKYRKRLDPPGSPLVNRVFLRDYVTTLP
                     LLVSIISLIVQTALTVQIVK"
     gene            12514..12972
                     /locus_tag="PLD98_01040"
     CDS             12514..12972
                     /locus_tag="PLD98_01040"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HQA04872.1"
                     /translation="MNPLINEMMQEALALAKEALENGEIPVGAVIVKNGEIVGRGRNK
                     REEKQSALSHAEIEAIDDACKNLGSWRLDGCHMFVTLEPCPMCAGAIATARIERVYFG
                     AFDPALGAAGSAINILEMKGAKVVDAFGGIMEAECAALLKEFFDKARSKN"
     gene            complement(12995..13591)
                     /locus_tag="PLD98_01045"
     CDS             complement(12995..13591)
                     /locus_tag="PLD98_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HQA04873.1"
                     /translation="MKTAELFLLAVGLSMDAFAVAVCKGLSVKKISILNIITVGIYFG
                     GFQALMPTIGYLLGSQFADSLRQWDHWIAFILLAIIGARAIKESFERDEREPFCGPEE
                     LPSGGKIKSPFGPALMFPMAVATSIDALAAGVSFSFLDINIVYAVCFIGIVTFTLSMI
                     GVKCGAVFGAKFKQRAEFTGGLILVLMGLKILIEGLME"
     gene            complement(13903..15198)
                     /locus_tag="PLD98_01050"
     CDS             complement(13903..15198)
                     /locus_tag="PLD98_01050"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HQA04874.1"
                     /translation="MRFMPLLFASVFDVFIGDMLFKWMGRRKVNDFKFLALSLLICAL
                     QILNIVILKHQAITTFVSMLIMFFLTQFYSMSFSLRLSGSITISLIMLLSELTVMVLT
                     SLGLGVDIREIPSNYLIHAICVLLAKFTAFILVNFLVSRQNHIRRGMSRKLTLSMLVL
                     PGSSLLVISLLYMYCYRISEEYLQVATFAASVLLAVANILAFHIINTQGDYISTKEQL
                     KFAKANIESQKEYYKELYEYQREISQFRHDMKNFFTAILSKLRAGESEEVEKRIAEKL
                     NFMDEFSRSIVNTGNPVLDSILTSKLKQSEACGIKTNISIKLSSPIRIDELELGVLLG
                     NALDNAIEAAVKAEGEKNINVSILTADETMLIKITNPVAKKVDVKNLETTKSDKKRHG
                     YGLSGIRTIVQRNGGILDLECTDTLFTFTATLSNAENQI"
     gene            complement(15203..15913)
                     /locus_tag="PLD98_01055"
     CDS             complement(15203..15913)
                     /locus_tag="PLD98_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQA04875.1"
                     /translation="MRICVCDDDPSVRNEVKDYILTFSIGISSSDITELSSGEALIKH
                     YAIKGRFDIIFIDVEMGGITGIAAAEKIRGIDPNVIIIFISNHAQYVFDSFYSEAFHY
                     ILKPIKKEEFAEVFNRALNKYMLMNTLIWLKWKGERFAVPIREIRYAKNSMRHVVLHT
                     EDGVRDANGSVSDILEEIEVYGFLKINQSCIVNIDYIKRLTDEEVVLKDNTVLSISVR
                     RRAAVLKAYEKFLHKMKW"
     gene            complement(16253..17512)
                     /locus_tag="PLD98_01060"
     CDS             complement(16253..17512)
                     /locus_tag="PLD98_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA04876.1"
                     /translation="MALNIRENIKMAFKSLRTNLLRSLLTMLGIIIGVGSVIAVITIG
                     TGGRDYVVNMIKEMGGTTAINVVVNTSKASLSDYITDEDIKAIKNNIDDVEYISPVVM
                     SVGAIAANEKNGIGFAIGTNTDIEAILGQSPAHGRVFNYQEYIAARNVAVIDAASAFM
                     LFGKENAVGEVIDYTFEGKTVSFKVIGVMNFMSAMGGDTQKMFEQMQPLMESGQAVGG
                     AMLLIPSTVATSLLGRGERYESIYLKSKDDSILESIGEAAVNILYARHNNADRNIYTA
                     TNLASFIELLDTVITVFTLFIASVGAISLIVGGIGVMNIMLVSVTERTREIGIRKALG
                     AKTKAIMLQFLTEALIICLTGGLLGLFFGTAVAAFVSAIMQVPLSVKFSTVILAIGFS
                     SAVGVFFGLYPAKRAAEMPPIEALRAQ"
     gene            complement(17531..19201)
                     /locus_tag="PLD98_01065"
     CDS             complement(17531..19201)
                     /locus_tag="PLD98_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQA04877.1"
                     /translation="MKFSIKKQKKTQNEEILTAPYDEVRAPKPKKEKKPLDKKLIATV
                     SAILAVLIAIGSIIGINVERKRVYVTLVEAKIGSVQEVYETAAQLTSSGQSVFYAYPG
                     VVVNDIFVKVGDTVKAGDVLASFDTTELQLEVQKKKAAYDLAKQAYEEAVRSAALAKA
                     ELPSVEAKIAQLEDKLKDSGADTGSSTPTNNNTVVVDAISKLFGESQIGQVLASLIQM
                     GASIQQINDMIQMFQNGGLGSGGLSALLGKQYQQLDDASQLMQLKTAKAIYEFQAGDL
                     YVASFKAVMDKAEKEYNELNNKLNELNSGWVASVSGKVSEINIQKGQKIESTSPQPDM
                     SSIDINTLLDMLSGNADISSLLSSFMLTGAETKNVKGITVENYDMFVATFQVGMYDAP
                     SIKVGMPATVEYMGKEFEGEVSYISATAKEGGLGLGSAIGSLTGGLTGSSSYVEAKVA
                     VKNPTEDLISGFNANIKINTRKAENTVIIPVDTLKLIDGEKYVFVYNKDDKRIYQRKV
                     ELGLSEETKYQVVSGLKAGEQIVRTFSSRTVKSLKDGMKVKVSEKIEN"
     gene            19462..20004
                     /locus_tag="PLD98_01070"
     CDS             19462..20004
                     /locus_tag="PLD98_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04878.1"
                     /translation="MIKKITAVLLSVIILFLAACSRPKRVDSLELSKRLEKIKESYAF
                     DSLDTYIVNDEHIVFISLVKANDCLLSYTTDGNNSITEIRLTIDAENWDKNDAAYIFG
                     EYAAAVLSAFSGCAYDEALNYAKEIGLFDESAYFNMHEAESSFDRYSIKMYANPLCAV
                     AEFIYSPSLVKTSEASETDA"
     gene            20407..21693
                     /locus_tag="PLD98_01075"
     CDS             20407..21693
                     /locus_tag="PLD98_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA04879.1"
                     /translation="MENISYSKAIPVRYEADVMVAGSGMAGIAAALGAAMEGMDVIIV
                     ERFGVLGGNATAGGVANFCGNNVGQGSVLDMVMAALEEFDAIAPRKKGRGGRVFDHEI
                     LAVILPELLERYKVKVLLHTTISDVVLNGRRIEAAIVTGPSGLEAIKAKQFIDCTGEG
                     ALARSAGFSVMKGGSSKYQLPMSLMYFVRHVEEKDVKYIPEGWFEKIDSEDKLPMTSI
                     WPNGPRSNAIKIKVPMYDSTDTVSMTMAEINGRRKMMSVLDYHQRQEKRSWILDHCST
                     VIGIREGVRIVGDYILTVDDLRNCKRFEDVIAVGTYYLDGHKPDDDKRTYILPKESLK
                     VPPYDIPLRCLIAKDGDNLMMAGRCLSADQLALSSARVMTTCSMTGQAAGITAALAVK
                     MNTDVRGVPYAEVQKKMIEAGAILDRDEVASFYEAE"
     gene            complement(21730..22302)
                     /locus_tag="PLD98_01080"
     CDS             complement(21730..22302)
                     /locus_tag="PLD98_01080"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQA04880.1"
                     /translation="MYTSFEELKNDCQGCTKCGLHATRTNVVFGTGNEKAKIMFVGEG
                     PGENEDLTGQPFVGRGGKLLDKYLNSIGLDRNKNVYIANIVKCRPPENRDPKPEEQEQ
                     CIPWLREQFRLIKPKLVVCLGRIAACRLISPDYKVTSQHGQIVMKSGTAFMGTFHPAA
                     LLRNPNNKEAALQDFIEIRKFIEKNLDMEL"
     gene            complement(22308..23648)
                     /locus_tag="PLD98_01085"
     CDS             complement(22308..23648)
                     /locus_tag="PLD98_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HQA04881.1"
                     /translation="MNMKDINLLYKDTIEKNFEGNMESSKRMWEYLENSTAKYKGRTI
                     YALYMPKLFTDEDVEYLRDKIETMYRILIKVINEYFEKEDYRELFGFDKELEELILNR
                     PSYSCLLPICRLDIFYNEEDRSFKFCEFNADGSSSMNEDRELNIAIKLTQVYKDLSDK
                     FEFSSFELFDSWAKTFLEIYNTSENPKEKPYIAITDFLEKGCSIEEFAQFKLAFEKAG
                     CKAEICEIRDFKFDGENLISPTGHKVDAIYRRAVTTDIFTHKSEIQPFLEAVKKRKVV
                     LIGDFCTQIIHNKILFYILHLDRTKAFLTEDEIAYIDAHVPKTVKLSDEFIEKLDVLT
                     AKDKWIIKPEDSYGAKGVYAGIHFSDEEWAELIRKHKDDSYILQEFIMPYASYNIDFK
                     KKNPEFKKYSNLTGIYLYGGKMAGFYSRQSVKEIISTAHDENDIASTVIRKKQG"
     gene            complement(23666..24931)
                     /locus_tag="PLD98_01090"
     CDS             complement(23666..24931)
                     /locus_tag="PLD98_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HQA04882.1"
                     /translation="MNKSENKAIAAAKAYIEGGIKSQYEKFSLGLELEHFLVDAKTLR
                     SIEYFGENGVAAVMEELSPHFETVVRKDEFVLALGNDDYTITLEPAAQLEISIRPCSD
                     IAEIVRIYKDFRCLIDPVLNKRGLKLINLGYHPVSKVDDLPLIPKDRYKFMYNHFAKT
                     GRYGKNMMKGTAATQVSIDYYSEKDFIKKYKIACRLSPFLSLLSDNAPVFEGAENKEF
                     ILRTKLWRDVDTARTDYCGFACDAGISFDSYADFVTASPLIVEYGETGCTTKSAQEIY
                     ADEEPAQEEVRHIFSMSFPTVRLKTYIEIRYADSLSPDLAVSYVALIKGLFENTEDIE
                     SYLNTLPISTLEDIKNAENEIMKKGLSAEISGITAADIIKKVFSVAEKNLNASEREYL
                     KPLKKLVCEEKRLPRETAATRLKECCSLV"
     gene            complement(24959..25738)
                     /locus_tag="PLD98_01095"
     CDS             complement(24959..25738)
                     /locus_tag="PLD98_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HQA04883.1"
                     /translation="MSEKPDSRMAVLIDADNVSCSYAKPMLEELARYGTPTVKRIYGD
                     WTSPNLSNWKAILLENAIIPVQQYSYTTGKNSTDSALIIDAMDLLYTGNLDCFCIVSS
                     DSDFTRLATRLREAGKKVIGIGEEKTPKPFIAACDKFIFLEILKPNVCKPVKIEKAPA
                     KSTGRGQSQSKLSPEPLPQELINLLVTSVEDIAGEDGWTFLGDLGNLILKKNPSFDPR
                     NYGYTKLTPLILATDMFDIDVRETSNKTVKHMYVRLKQSEG"
     gene            complement(25754..26917)
                     /gene="ytvI"
                     /locus_tag="PLD98_01100"
     CDS             complement(25754..26917)
                     /gene="ytvI"
                     /locus_tag="PLD98_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HQA04884.1"
                     /translation="MNRTERRRKFIIDVFFWLIIIAAFYLFMKIAFWYVFPFVFAFFL
                     TMSLQKPIEKITKVTRLKPGFVGTALVLLITAIALFIVFAIGVKLTNELKSFAQYLTQ
                     RVNDIPAIVTEVENWLLRVISFLPDSLEQAAANFIRKYAESIRVPGVPDSSLDLSWLK
                     SPLGSVWETAKQIPSIVLALVVTVVSSCFMATSYKEIKEFIYRQISEEKGKKLSQTKT
                     VLFSTLGKMAKAYCLLMLITFSEISIGLGLLKVAGIYEVNYILVIAIVTALVDIVPVL
                     GTGTILIPWSAYLLITGEFGGGIGLLVMYAIITVIRQIIEPKLVADQVGLPPVVTIMS
                     MYIGAKVFGPIGIFLLPIIVILIKLLNDKGIIKIFKTSNSEHAAEETAKEESV"
     gene            complement(26978..27661)
                     /locus_tag="PLD98_01105"
     CDS             complement(26978..27661)
                     /locus_tag="PLD98_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA04885.1"
                     /translation="MSLIELNNISKIYNPGENEVRALDAVSLEIDFGEFVGIVGQSGS
                     GKSTLMNILGLLDVPTSGTYKLDGKYVDELDDDSLSEIRNKEIGFIFQGFNLISNLTA
                     RENVELPLVYRGMPADERRELSRVALEKVGMLHREKHLPSQLSGGQQQRIAIARAIAA
                     APPIILADEPTGNLDSKTGDEVMRILHELNAEGRTIVLITHDNQIASGIKRNIRIHDG
                     RIVDDYFNF"
     gene            complement(27745..28950)
                     /locus_tag="PLD98_01110"
     CDS             complement(27745..28950)
                     /locus_tag="PLD98_01110"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HQA04886.1"
                     /translation="MKYVVVLYDGMADMPVAELGGKTPMEAANKPLFDSLGKKGEAGL
                     VKTIPDGMNPGSDVANLSVLGYDPRQSYTGRSPLEAVSIGVNLSDTDVTLRCNLVTLS
                     DEENYEDKTMIDYSGGDISTAEAKLLIDAIQEHFGSDIYEYYSGVAYRHCLVWHGGTV
                     DLGRMTPPHDISDRVIGPYLSKSENAADLIRMMKESYNILKDHPVNKKRIAEGKRPAN
                     SIWLWGEGTKPNLPLFKDLYGLDGSIISAVDLLKGIGGLAGMSTPTVEGATGYLDTNF
                     EGKAQTAVDELKNGKDFVYIHIEATDECGHRREIHNKVKAIEEIDRRVLPIVLEGLKE
                     FDDYKIMILPDHPTPLATGTHSTDPVPFMIYHKNNEKQGVDTVTEKTAAATGLYFGSG
                     AELMKHFLA"
     gene            complement(29023..>29680)
                     /locus_tag="PLD98_01115"
     CDS             complement(29023..>29680)
                     /locus_tag="PLD98_01115"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04887.1"
                     /translation="EKEEFLDLNAQINGIPLVIDAIRCGDKIAVNQCSMGLDAEICAK
                     QASFKKLPGMKAESAYYAALLYCFLRKIKNEFTIQTEGEEERKLTVLFCLCANSRWYG
                     GGFMGAPLAIPDDGLLDFVIVRKEVSRAKMLGLVDQYKKGQHLSLPFTEFKRGKKITV
                     KSEKPAAVNIDGEVEIVTESTFEIVPKSVKFVIPSTSTYFDRVKSGEINPDTPLPVEE
                     "
BASE COUNT         7097 a         7028 c         7260 g         8295 t
ORIGIN      
        1 tcttccggca aagtaaccgt gagaaaaacc taatgtctgg aaacccttta tttataagtg
       61 ttttgaatac ccctacttct caacccttga catcaatacc acgcactcaa cgtgcggggt
      121 gcggggaaag aggtcgaggg gggtgcagga ggagagggtg tagccctttt ccttaaacag
      181 cttgcagtcg cgggcaaggg tggcggggtc gcaggagata tagactattc tctttgggtt
      241 tatctgggct attatatcga taagctcggg tgcgcagcct tttctcggcg gatcgagaag
      301 cagcacatcg ggctgttcgt ttctttgaag caggatttcc gccgcggttt ccgctgagcc
      361 gcagatgaac tcggtattct caatgccgtt ttccaccgcg ttctttttgg cggtttccac
      421 ggattcctca acaacttcga tgcctatgag ctttacattt ttgagtcccg attctttaat
      481 cataaaaaga ccgattgtgc ccgtgccgca gtacaggtcc acaagggttt cgccttcttt
      541 aaggttcgcg agcttagctg cttttcggta aagcctttcc gcctgagctt tgttaacctg
      601 aaaaaaaacc tgtggcggga gttttacatt aagctctccc agcttgtccc gtatcgcttc
      661 agcgccgtaa accaggctgt actcatcgcc cagaataaca ttagtgtcct ttgtgttctt
      721 attaaggtaa acgcttttga cggcggggaa tttctcgctg actctcttta taagaatatc
      781 cttgtccttt tcattaagag ttccgttcag cacaaggcag agcatgattt cgccggaacc
      841 gaaacctttc ctgagataaa tatgccttac aagacctttg cctgtaagtt cgtcataggc
      901 ttttattttg ttttcccgca tccactgtct gacttcgttt actatttccc caaattcaga
      961 aggctggagg cggcagattc cgccgcactc gattattctg tggcttctgg gagcgaaaaa
     1021 gcctattctt atctcgtcct tttcctctct gacggggaac tgggctttgt tcctgtaaaa
     1081 gtttacatcg tcagcgggaa taacaggctc tacctctatg tcaatgccgc ctatgcgctt
     1141 gaaagcgtcg acaaccgtct gtctttttat tctcagctca gcctcatagg aaatatggcg
     1201 gtaggcgcag cctccgcatt tgtcgctgac ggggcagtcg gactcgattc tgtcagggga
     1261 aggggttatg atgttctcga ttttgcccac agcgtagttc tttttaactt ttatgatacg
     1321 gcagtcaagc acatcgccga ttgcgccgtt ggggacaaaa acagcaaagg agtcaagtct
     1381 gcccacggcg ctgccctctg aggtcatgcc gtctatgtta agtcttatta tgtcgttctt
     1441 ctttaagggt gctgtcattt ctaccttcct atatttttaa agccctctcc ttatggaaag
     1501 ggctttaatg ctaaaactca atctcaagcc catcgtaagc tacgatgaag cctttttcct
     1561 gggctacggg caccagctcg tcgtgaatga gcttgccgtt gtgggagaag tggttgatta
     1621 caaatatggt attctcgtcc gcgcagccga ttttgataag cctttctttt acttcctcat
     1681 tgcctttaag gcccatgtgg ctgccggggt aatctataag catgcctgtg cagtcaaggg
     1741 agacaaagtc aaaatagggc ttattcttct caagatactc ccatgtggcg tcggtgaaat
     1801 agtcagtgtc atgggcgtag agaaggcact tttcgccctt aataatgtaa atcagcggat
     1861 ccagaacaac gctgtgcttt gcctcaaggg gagtgatttc aaagccttct gcctcgaatt
     1921 tctcaaacgc ctttatttcg tgaagtttta aagctccgca ctcttcaagg ctttcatact
     1981 cgtcatagcc ccttattatg tcgcttgtct tggggctggc gtacatatga agggttgcgg
     2041 gggcgttttt gtccgcgtcg gcgtaggcaa agcccttttt gcggatatgc atttcttcaa
     2101 ggtacagatg gtcggaatga ccgtgggtta taaggcaggt atttatgtta tgagcgtcaa
     2161 agccgcccgt tactgcgtgc atattggtgt cgggaccgaa gtccagaagg agtttattat
     2221 ccaccaaagc ctgcgcgcgg gttctcaggt ttttgccgcc tgcttttctc gctctttcgc
     2281 agacatcgca tttgcagaaa agaccgggaa agccctcgga ggctccggtg cccaggtatt
     2341 taattttcat tttgccacat ccgtttctat tcgtagttta gagggttttt gcgagctcct
     2401 ttaaatactc cttgaaagac tcgccgattt cggggtgctt taaggcaacc tcgcagtttg
     2461 cctgcatgat gccgagtttg tcgcccatgt cgtatctctt gccaacaaag tcaacggcgg
     2521 tgagtctgcc gtgctcggcg agatagctga gggcgtctgt aaggtaaacc tctcccgact
     2581 tcgggttggg aggagtattt ctgataaggt caaagatttc gggctcaagg acattcctgc
     2641 caaggatagc gtagagggac ataatctcgt gctcttcggg cttttctata atcttgtgga
     2701 catccattac attgccctca aggggtgtta catcaagggt gcagtatttc ttaatctgct
     2761 ctcttgtgca ctcgtttacg cccacgcagg atttgccgta cttctcatat gcctttataa
     2821 gctgaccgac aacaggctcc tcggagatga taacatcgtc gccgtagaga accgcaaagg
     2881 gctcgtcgcc aacaaagctt tcagccttta aaatggcgtg acccagaccc ttggtttcct
     2941 tctggcggat gaaataaatg ttggcaaggt cggctatatt gtgaacagcg tcataaagag
     3001 gcttttttgc gggatttgcc ttgaggtttt cttcaagctc aaaggcgtag tcaaagtgat
     3061 tctctatgac ctccttgccc ctgttggtga tgatgaggat gtcggtaatt cccgccgccg
     3121 cggcttcctc aacaaggtac tgaatcgcgg gcttgtctac gatgttcagc atttccttgg
     3181 gaacggcttt tgacgcggga agaaccctgg tgccaaggcc tgccgcagga attatcgcct
     3241 tagttatttt tttcattgca atctcccttt catatgctaa tttcacaggc gtgtatttac
     3301 gcgagaattg atatcacaag tagttttacc gccgtatcaa tgaaaaagta cacaacggcg
     3361 gcaatcagcg ccgaacccaa agaggttccg cccatttcgt aaaggagtct ttggtactga
     3421 gcgggctcgg tcgccgtttt gcgggccttg tttatcctct ttttaatatc gcccagatac
     3481 aggtaatcgc caaagctggc aagaccgaaa atagcggcat gggcaaggat gttgaggaca
     3541 ttttttcttt ttaattcttc cgaggtgtag cccgactttt gaattacttt atcgataatt
     3601 tcctgaaact gctctacgct gattatgccc gaattctcca taacctgttc ggaggcattc
     3661 tttatctcat agtaaagcct tgcgtcagag ggactgatat tcgctaccga gaaaaacagc
     3721 gcgcccgcca gcacaagagc tcctattttg ggcatttttc tgtagaagaa ccaaagggcg
     3781 gggaagaaaa gggatatagg gttccaaaat atgctgaaaa gaagcgccaa aatgcatacg
     3841 ccccagttcc agcctatttt tacattggtc tggtcaatcc tgaaaaagcg gctgagatag
     3901 cggggcacct ttgtctgtac cacctgagcg agttctattg cggggattcc gtcaatcaaa
     3961 gcctccgggg gaatggcggt tgcgtaggca aaagagggtc tttcaaaggg gctcctcggc
     4021 tcctcgccgt aggggtcggc gctttccctc cttgcgtcgt ttcgctctcc cgccgtaaaa
     4081 ctcggctctg agttgggcgt ttcttttctt ttctcctcct ttacagagga ggtccaaaca
     4141 tagccctcat catgcaaatg ctcgtttacg caggcgttgt ttttccgcca gcattctctg
     4201 tgctgaggag tgccgcaaac gggacatacg actatgtcct catccttgtt taaaggcttt
     4261 gaacagcccg ggcaaatatc tccgttagtc cacatcgtga atcctttctg tgttatattt
     4321 aacactcaac agatttttct tctgttttct tctttttatg gaagtagatt tgaaccgaaa
     4381 ccaccgcgat tattgagagg gcggcgacca gaattctggg aggagtgatg gtcagacccg
     4441 ctactccctc ctgaagggcg gtgaagttct tttctatgtt gaccgccgct ctttccagag
     4501 ccgcgcgggc ggtgaggtaa tcatactttg agcgcactct gccgctgagg tactcctcgg
     4561 cgtcgctgac cgcccacaga taggcgcgct tatagtcgcc gaaggtatag gacttaaact
     4621 cgggcagcac ccactgctct ttgtatctct tgtaagcctc tctgagctga gttttcgcgt
     4681 actcggtttc ttttgcgtac tcagaaagct cctcggggta gtcggtgatg attatgctgt
     4741 atcctctgga aacaacatcg ttccaccatt tttcggtgtc ctctctgctg ccgcagaggt
     4801 tggggtcggt aaggtcaatg gctgccctgc cgacggactc aaacttcttc attgtgaagt
     4861 tatcgaaaat cacgccgtag gggttcttgg tggcaagcct tacagcctcc gctccgccct
     4921 caaaggattt ctttatatat gagcctatca taaagactat attgctcttg tagccgctca
     4981 ttatgtgagg ctttacttca agacccgacg cccattcgcc gatgtccttc gcctttgcct
     5041 cggtgtagaa aatcacatcg ccgagcatat ccctgtcttt aaccacatta taaagcacat
     5101 ccctgagcgc ccagtcaaaa tcgagcatga gcatgccctt gccctgaacg gctccgagaa
     5161 catcgtacag aaaaggcact tcgaagtttg tttgtctgct catcttctcg ccgacgccgc
     5221 ttaaaaggcg gtacttctta atttcctcgg cggtgagctc ttttaagggc ttgtcgtccc
     5281 cgccgtagca ggttctgccc gttgtgtcgt ccgccataag gacaaaattg ccgtctttga
     5341 gctgctttac attaaccttt actatgtccg ctcccttttc aaaggcggag aggacagcct
     5401 caagggagtt ttcggggaag tttctccagt cgcccttatt ggaaaccaca agcaggcggc
     5461 cgttggggtt tgaaaactcg gacgcggcgt caaaggcgtt tttgtccttt tctctttcgt
     5521 cttccttatc caggtcgctg ccgtagtagt caaaatagtt tgtggtttcc tcctcatccg
     5581 caagggcggt gaggggaagg gcaaatacag cggccagaca cagtattatg caaatcagtt
     5641 tatgaaattt attcattttt ataccatcct aaaacttgct ttgccgtcgg cagtttttta
     5701 ttatatcata ttaaagcagt tttttccaca agcaaaaaga ctatttgcgc cgcctttcag
     5761 cctttttcga agggcgctct ttttatttta tgtatatcgg tttgtctgtt atttcgacgc
     5821 tgcgttttgc gcttgactca agctccagaa tgataatctc gttttcctct ttaagcaggg
     5881 ctccggggag atagagggtt ctctggggac cgattttcca gtatctgccg aggttaaagc
     5941 cgtttaccca gactatgccc tttgtaaagc ccttcatgtc cacaaagcag tcggcgtttg
     6001 tttccgcctt aaagctgccc cttaaaaaga gggggtactg agtatctttg tttccgaatt
     6061 tgatttggct taaatcctca agcggcaggg tgtagataag ccagtcggag aggttttgat
     6121 tgccgagttt tgcgttcttt atgcccttgc ggtcgtagat gaagttaccg tagtttactc
     6181 tgcccatcgc ttcaacaagg atgtcgagac gcatcttgcc gctgaaggcg gggaagtgaa
     6241 cgataaagca ctcggagtat tcgtttctgc cggttttgtc ggtgcggcag aagctgcctt
     6301 tgaactcgcc gttaatccat acaaaagctt tgtcgtgaat gccctctatt gtgattttgt
     6361 cgtcgttgta attgccctca atttcggtgc tgtaaaggat aagtccgtgg ttctgaccgt
     6421 agtactccat ggagcggggc gaaacggagc ggtgcttttt gccgagtctc tttatgttgt
     6481 ttaatagcga ggccgtttcg gtgagattta cctttccgat tctctgcaaa acgggaggag
     6541 cggggggctc gggcaggctc tcccccgtct tttcaattat tgcctctctg acggcaaagt
     6601 atttgtccgt atagccgccg tactcattga gcggagcgtc atagtcgtag cttgtgacgg
     6661 tgggggtgta tttgtcgtag tagttggcgc ccgatgtaaa gccgaagttg gtgccgccgt
     6721 ggaacatata aaggttaaag ctggcgttca agtcaaaaaa ctgcctaata gcctcgccca
     6781 gtttctccgc gctgcctttc gggtgctttc ctccccatgc ctcaaaccag ccgcaccagt
     6841 actcgccgca cataaggggc ttgtccggct gcagctctct gaggtcttta aaggagtctg
     6901 cgggcattga attgaaattg gtgaccatga gctctccctc aagaccgccg cccgagagat
     6961 agtaacggtt gtcgccgtcg gaggtgaaaa gcagcacatc aatgccgttt gagcgcataa
     7021 gcccgcggag atacgacaga taattcttat cgttgccgaa gctgccgtac tcgttttcaa
     7081 tctgcatagc gattatgggg ccgtcctttg tcttttgaag gggcgcaagc atgggcaaaa
     7141 gcacattaaa atagtcggtc acggctttaa gatatttttt gtcgccgcag cgaaggcgga
     7201 tattctcgtc ctttaaaagc catgcgggaa agccgccgaa atcccattcg gcgcagatat
     7261 aaggccccgg gcggacgatg acattcagac cgattttctc cgccgtctta acaaacctta
     7321 ctatgtcaag aatgccgtca aagttaaact cgcctttttt tggctcgtgc aggttccacg
     7381 ggacataggt ttcgacggtg ttaagccccg cggctttgag tttaagcagt ctgttctccc
     7441 agtattcggg caggactctg aaataatgaa tcgctcctga gcggataatt gtctttttgc
     7501 cgtcaagata aaaatcacgg ccgcgaatct ctaaaatatg cctcacctct tgaagatact
     7561 aatagcataa tagcccatat ccttttaggt gtaaagacaa aaagcggggc aaaacgcgcc
     7621 ccgctttgtg gtattatgtt atgctgtggt gtcggttgaa gctgaaagaa cgatgatttc
     7681 cttttctccg acgagctgca cgggatagga ttcggcaata tagccgtggt gctttattat
     7741 cagctcatcg ccgattttta tgccgtcaaa atctattttt tcgccgtctt ttttaacgct
     7801 tgccttttca gacagcacat aggcaaggtc ggaaaaatct acggggcttt caatgacttt
     7861 aacaagtatg ctgccgctgt acttttcctt tacgacggct ttgaattcaa tattctcaag
     7921 ctctgaggcg ttccttcttg tggtgtccgc ctctctgccg ccgctttcct tgcaggcggc
     7981 aaggcttaac agaagtgaaa atattattat caaagggaca agtcttttaa tcatatttag
     8041 tcaccttgta tcaatatatt caacaattag agatttgcga ctttcgtttt gtcccagctt
     8101 tcgggcgtaa aaaagcagac caaaaaagaa atctttcagt ctgctttgga gctggtggtc
     8161 gggatcgaac cgacaacctg ctcattacga atgagctgct ctgccattga gccacaccag
     8221 cgggtttaat tgttcatgcc gcggacggtt atgacgggaa aatatcgtga cccttttttc
     8281 tttcgttcct tttacaaaag gggacgggat acttggggtg ggttttgtgg tgctctttgc
     8341 actcctcgca atttccgtgc cttttgcatg atttccattt gcaaatgcat tctgtcattt
     8401 ttgtaccctt ttctttcgga aacccgcaaa ggcgttaagt taagtttaat tcccgcatag
     8461 tacttttagt ccgtcaggcc gatttattat agcaaattta aatgtcataa tcaagatttt
     8521 aataaaaaat atgcagaggg aagttgtgcc gctccccggt ttattaatca aatgtttact
     8581 tatatggttt gatttttggg gttcatcgtg gtaaaattat acggagtaac taaaagcaaa
     8641 tattacttga agtttctata tactcaaatt aaggaatgat aagattgtca atacttaaaa
     8701 agattttcgg agactattct caaaaggagc ttaaaagaat taagcccctg tgcgataaga
     8761 ttctggctct tgaaagcaca tacgaaaagc tgactgacgc tgagctgaga gccaaaacag
     8821 aggaattcaa aaagcgttat caaaacggcg aaagccttga cgctcttctg cccgaggcat
     8881 tcgcggtctg ccgcgaggcg tcgtggagag ttctcggcat gaagcacttc cccgttcagg
     8941 ttttgggcgg cattattctg catcagggca gaatagccga gatgaaaacg ggcgaaggca
     9001 aaacccttgt ggctactctc cccgcttacc tcaacgctat cgcgggtgag ggcgttcata
     9061 ttgttaccgt caacgactat cttgcccgcc gcgactcgga atggatgggc aaggttttca
     9121 gatttttagg tcttaccgtc ggtcttgtta taccctccat tccccatgac cagaaggcag
     9181 ccgcttacgc cgcggacatt acctacggca ctaacaacga gttcggcttt gactatctta
     9241 gagacaacat ggtcatgtat aaggagcaga gggttcagag gggtcacaat ttcgccatcg
     9301 tggacgaggt ggactcgatc ctcatagacg aggcaagaac acctctgatt atttcgggca
     9361 gagccgacaa gtccaccgac ctttacaaaa tcgccgacaa ctttgtaaag ggtcttaaat
     9421 gcgtaaaagt caccgagctt tcctccaaga aaaacgctga ggacgaactg gaggaggaag
     9481 gcgactacat cgttgacgaa aaggcccgca ccgctactct gactaagagc ggtgtcgcaa
     9541 aggctgagaa attcttctcc cttgaaaacc ttatggacgc cgagaacctt acaattcagc
     9601 accatattaa cctcgccatt aaggcaaggg gcgtcatgca gcgggatgtt gactatgtgg
     9661 ttaaggacgg tcaggtgctg attgttgacg agttcacggg ccgaatcatg ataggcagaa
     9721 ggtactccga gggtctgcat caggctatcg aggcaaaaga gggcgttaat gtccgacatg
     9781 agagcaggac tctcgccacg attaccttcc agaactactt cagactctac aaaaagctct
     9841 cgggcatgac gggtacagcc atgaccgaaa ggcaggagtt tgaggagatt tataacctgg
     9901 atgttgtgga aatccccaca aacaagccga tgataagaat tgaccacccc gatgtgctgt
     9961 ttaagaccga ggaagctaaa ttcaacgctg ccattgacca gattgtggcc tgccacgaga
    10021 agggtcagcc ggtgcttgtg ggtacggtca gcattgaaaa atcggagaaa ttcagcaagg
    10081 ccctcaaaag gcgcggcata aagcacgaag tgctgaacgc caagaaccac gagcgcgagg
    10141 ctgaaatcat agctcaggcg ggcagactgg gcgccgtcac catcgccacc aacatggcgg
    10201 gccgcggtac cgacattatt ctgggcggca actgcgagta tatggcaaaa gcggaaatgc
    10261 gcaagatggg cattcccgag gagcttatcg cggaatccac cagctttgcc gaaaccgacg
    10321 acgaggaaat cttaaaggca aggcagatat ttaaagagct ggaggcaaag ttcaaagagc
    10381 agcaggctgc cgaggctgag gctgtgcgca aggcgggcgg acttttcata atcggcaccg
    10441 agcgtcacga gtcaaggcgc attgacaacc agctcagagg acgcgccggc cgtcagggcg
    10501 acccgggcga gagccgcttc tacctctccg cagaggatga ccttttaagg ctctttgcgg
    10561 gcgaccgctt ctacagaaca cttgatatgt tcaagtccgc aggcgaaatg cccattgacg
    10621 cgaaaatctt ctcaagcgca attgaaagcg cgcagaagaa ggttgagggc aacaactttg
    10681 ccatccgccg caacctgtta aagtacgacg atgtaatgaa tcagcagcgt gaaatcatat
    10741 ataaacagag gaacatggtg cttgacggcg tggaccttga cgccaccatt cagaagatga
    10801 ttgaggaaag catcaggtct gccgttgatt tctactgcgc caagaatata ccctccgagg
    10861 actggaatct tgacggtctg aggagcaagt atgcatggct cttaaagaac aaggaagatg
    10921 taaactccga catgagccat gatgaggttt acgaaacact cttgaacctt gccatggaga
    10981 ggttcaatca tcaaaaggct ctttacggcg aggaattaat gcgggctctt gagcacaaga
    11041 ttctccttga gaatgttgac attcgctgga tggagcatat cgacgccatg gagcagctta
    11101 agacgggcat cgggctcagg gcctatgccc agaaggaccc cgtcgtcgcc ttcaggcagg
    11161 agagctttga tatgttcgag gagatgaccg agaacattcg cgacggcacg gttcagatgc
    11221 tgcttacagc cgttgtgcgc actgagaagg acacagagcg ccagcagcag gctgagatta
    11281 cctctcaaag cggcggcgac ggctccgaca agcagcgccc cgtaaagaaa aaggagagcg
    11341 aaaaggtagg cagaaacgac ccctgcccat gcggcagcgg ccttaagtac aaaaagtgct
    11401 gcggaagata atccttgcaa tgccataagg gcgctggaga aaggatgatt ttatgaacaa
    11461 cattaacccc ggcggcttcg gttttaatcc cccaccccat tacggcgctc agcccgtggg
    11521 cgttccattg gttccgcctc aaaatccccc tttgccgagg cgcagccgct ttgaccttag
    11581 gcgtcttagc atgctaacgg gacttagcat aatagccttt cttataatgt cctatgcctt
    11641 ttcggggata atcgtccttt tcgcggatga tttaaaggcg taccttaaag gcatttcgat
    11701 ggacacatcc attttctctt atatactcga gtccttgctt tccgtattcg gcattggtct
    11761 tcccttttat attgcctata gggcaatgag aaaaaagtac ggcgtggact ttatcccctt
    11821 ctccccgccc gaatcctata cactgatgct ccttatgata ggagccgcat tcgccgcctg
    11881 ccttttgggc agcagcttta catctttcct gaccattttc cttgaggaga acttcggaat
    11941 tacctccacc tacaccatgg gcgatatgcc cgtgacattt gacggaaagg ttctgtactt
    12001 tgtaaggtct gctatactcc ccgcgtttat tgaggagttt gctctgagag gcgttgtaat
    12061 gcagccgctc agacggtacg gaaactcttt cgccataatt gtttcttcga taatctttgc
    12121 gattatgcac ggcaatttga ttcaggctcc ctttgccttt attgcgggaa tcgctctggg
    12181 ctacgctgtc atcatcacca actccatatg gacgggagtg gtggtgcact tgcttaacaa
    12241 ctccctctcc gttatcctta tgttcctcac aaaaggcaat actgaggcgc aggtgccctt
    12301 ctatgtggcg gcgattatgc ctaccattat ctttataggt cttgtctgct ttgtgattta
    12361 ccttttcagc aagaagtaca gaaaaaggct tgacccgccc ggcagcccgc ttgtaaacag
    12421 ggttttcttg agggactatg tgaccaccct gccgcttttg gtttcgataa tctcccttat
    12481 tgttcagacc gctctgacgg tgcagatagt gaaatgaatc cccttataaa tgagatgatg
    12541 caagaggcgc tcgccctcgc caaagaggct ttggaaaacg gggaaatccc ggtgggcgct
    12601 gtgatcgtga aaaatgggga gattgtgggc agaggaagaa acaagcgtga ggaaaagcaa
    12661 agcgctctga gccacgcgga gattgaggcg atagatgacg cctgcaaaaa tctcggctca
    12721 tggaggctgg acggctgcca tatgttcgtg actttagagc cctgccccat gtgcgcggga
    12781 gccatagcga ccgcaaggat tgaaagggtt tatttcggag cgtttgaccc cgctctcggc
    12841 gccgccggct cagcgataaa catcctcgaa atgaagggcg caaaggtcgt ggacgctttc
    12901 ggcggaatta tggaagcgga atgcgccgcg ctgttaaaag agttttttga caaggcaaga
    12961 agcaaaaatt aaagcccctt ataggggctt tttgttattc cattaaacct tctatcagga
    13021 ttttaagacc catcagcaca agtatcagtc cgccggtaaa ctccgccctt tgcttgaatt
    13081 tcgcgccgaa aaccgcgccg cattttacgc ctatcattga tagggtgaag gtgacgattc
    13141 cgataaagca gactgcgtag actatgttaa tatccaaaaa ggagaaggaa actcccgcgg
    13201 caagggcgtc tatgcttgtc gccaccgcca tggggaacat taaagcggga ccaaaggggc
    13261 ttttgatttt tccgccgctc ggcaattctt cgggaccgca aaaaggttcg cgctcatccc
    13321 tctcaaaaga ttcctttatt gctctggctc cgattatggc aagcaggata aaggcaatcc
    13381 agtggtccca ctgcctgaga ctgtcggcaa actgggagcc gagaagatag ccgattgtgg
    13441 gcattaaagc ctgaaagccg ccaaaataaa tgccgaccgt aattatattc aatatgctta
    13501 ttttctttac ggaaagaccc ttgcagaccg ccacggcaaa ggcgtccatt gaaagaccca
    13561 ccgcaagcaa gaatagctcc gctgttttca ttttttcctc cggattttaa atctttaagg
    13621 tttgccgctt tgatattaaa tatacacgct ttatctaaaa tattcggttt gagcgcataa
    13681 tagaataaaa ggcataaaac aaaggtaaat ataacacagc gcataatgtc agtcaatgtt
    13741 tattgtatta ataatttgca accaaaaaag ggaaggaata atccttccct tgaattttct
    13801 gattaatgcg gtgtgccgcc ttggggaaaa tatcggaaag agtttagggc ggcttttctt
    13861 tcccgacagt cgggcggagc tgaattcggt gatagcttta agtcagattt ggttttccgc
    13921 gttgcttaaa gtggcggtga aagtaaagag tgtgtcggta cactcaaggt caagtattcc
    13981 gccgtttctt tgaactatgg ttctgattcc gctaagacca tagccgtggc gttttttatc
    14041 gctcttggtt gtttcaagat tctttacatc cacttttttc gctacggggt ttgtgatttt
    14101 tataagcatt gtttcgtccg cggttaagat gcttacattt atgttttttt cgccctctgc
    14161 tttgacagcc gcttcaatgg cgttgtcaag ggcgttgcct aagagtacgc ccagttccag
    14221 ctcgtcaatt cttatggggc ttgacagctt tatggatatg tttgtcttaa tgccgcacgc
    14281 ttcggactgt ttcagcttgg atgtcaaaat cgagtcaagc acggggttgc cggtgtttac
    14341 aatggagcgg gagaattcgt ccatgaaatt cagcttttcc gctattctct tttccacttc
    14401 ctcactctcg cccgctctga gctttgacag aatggcggtg aagaagttct tcatgtcgtg
    14461 cctgaactgg ctgatttccc tttggtattc gtaaagctcc ttgtaatact ccttctggct
    14521 ttctatgttc gccttagcga atttaagctg ctcctttgtg cttatgtagt cgccctgggt
    14581 gttgataata tggaaggcaa gtatgttcgc caccgcaagc agcacggacg ccgcaaaggt
    14641 tgccacctgc agatactctt ccgatatcct gtaacagtac atatacaaaa gggatataac
    14701 aagcagcgat gagccgggga gaacaagcat tgaaagcgtc agctttctcg acataccccg
    14761 ccttatgtgg ttctggcggc tgacaaggaa gtttacaagg atgaaggcgg taaacttggc
    14821 aagcaagacg caaatggcgt gaatgagata attcgagggg atttctctga tatccactcc
    14881 cagacccaga gaggtcagca ccatgaccgt aagctcggac aaaagcatta taaggcttat
    14941 ggtgatgctg cccgatagcc ttaagctaaa gctcatactg taaaactgag tcaggaaaaa
    15001 cattataagc attgaaacga aggtcgttat cgcctgatgt tttagaataa cgatattaag
    15061 gatttgcagg gcacagataa gaagacttaa agccaggaac ttaaagtcgt ttactttccg
    15121 cctgcccatc catttaaaca gcatgtctcc gataaaaaca tcgaaaaccg atgcaaaaag
    15181 caggggcata aatctcatgg tctcaccatt tcatcttgtg caaaaacttt tcgtaggctt
    15241 tcaaaaccgc ggctctgcgc ctgacgctga ttgaaagaac ggtgttgtcc tttaagacaa
    15301 cctcctcgtc ggtcagcctt tttatgtagt cgatattgac tatgcagctc tggttgattt
    15361 ttaaaaagcc gtaaacctca atctcttcca gaatatcgga aacgctgccg tttgcgtccc
    15421 ttacgccgtc ttcggtgtgg agaaccacat gccgcatact gtttttggca tagcggattt
    15481 ccctaatcgg caccgcgaag cgctcgccct tccatttgag ccagataagg gtattcatca
    15541 gcatgtactt gtttaaggct ctgttgaaaa cttcagcgaa ctcttccttc ttaatgggtt
    15601 ttaagatata gtggaaggct tcggagtaga atgagtcaaa tacatactgg gcgtggttgg
    15661 aaatgaaaat tataatcaca ttcgggtcga tgccgcggat tttctcggcc gcggcgattc
    15721 ccgtaatgcc gcccatctcc acatctatga agattatatc aaaacggccc ttaatagcgt
    15781 aatgcttaat aagggcctct ccgcttgaca gttcagtaat atccgaggag gatatgccga
    15841 tgctgaaagt cagaatatag tcttttacct cattgcgtac cgatgggtcg tcatcgcaaa
    15901 cacagattct catatcctca cctcaagctc ggagttttca gaccattaac cgcattcaaa
    15961 gacagcccgt caccttaaaa gcggggcgtc ttttagtaaa caagaaattt actaagagct
    16021 taaatatttt tcatattaag caaatacttt aataattttg actcttaacg ctatatcagg
    16081 tgacattata acagattttt cgagcatagt aaagcatttt tgccgaaaat tgtcaatgct
    16141 tttacttttt tctgttagtt ttgaataatt attgggcatt attacaaaca agacttaaat
    16201 cagcaatata atcataaaaa aattaagcac cccgtttttt ggggtgcgtt ttttactgag
    16261 ctctgagagc ttcgataggc ggcatttccg ctgctctctt tgcggggtag agaccgaaga
    16321 aaacgcctac cgcgctggag aagcctattg cgagaattac cgtcgagaat ttgacgctga
    16381 gggggacttg cataatcgcc gaaacgaacg cggctaccgc cgttccgaaa aacagtccca
    16441 aaagtccgcc ggtaaggcag attataagcg cctccgtcaa aaactgcagc ataatcgcct
    16501 ttgttttggc gccgagagct tttcttatgc cgatttccct tgtcctttcg gtgacggaga
    16561 caagcattat gttcataact ccgatgccgc cgacaataag ggaaatagct ccgacggatg
    16621 cgataaagag ggtaaagact gtgattacgg tgtcaagcag ctcaatgaag cttgcgaggt
    16681 tcgtggcggt gtagatattc ctgtccgcgt tgttgtgcct tgcgtatagt atgttgaccg
    16741 cagcttcgcc tatgctttca aggatgctgt cgtccttaga tttaaggtaa atgctctcgt
    16801 acctttctcc cctgccgaga agggaggtcg caacggtgga ggggatgagc agcatggctc
    16861 cgcccactgc ctgaccgctt tccattaagg gctgcatctg ctcaaacatc ttttgagtgt
    16921 cgccgcccat ggcgctcata aagttcatga cgcctattac tttaaaggaa acggtcttgc
    16981 cctcaaaggt atagtctatg acttcgccca ccgcgttctc cttgccgaaa agcataaagg
    17041 cgctggcggc gtctatgacc gccacatttc ttgcggcgat gtactcctga taattgaaca
    17101 ctctgccgtg ggcgggggat tgaccgagta ttgcctcaat atccgtattt gtgcctatgg
    17161 caaagccgat gccgtttttc tcattggcgg caatggcgcc aacgctcata acgacgggtg
    17221 agatgtactc cacatcgtct atattgttct ttatggcctt aatatcttca tcggttatat
    17281 agtcgctgag gctcgccttg cttgtgttta ccaccacatt aatggcggtg gtgccgccca
    17341 tttcctttat catgttgaca acatagtcgc ggccgccggt gcctatggtg atgacggcga
    17401 taacagagcc gacgccgatg ataattccca gcatggtaag gagcgacctt aagagatttg
    17461 tcctaaggct tttaaaggcc attttaatgt tttcacgaat gttcaaagcc aaaacacctt
    17521 ccgccctgcc ttagttttca attttctcgc ttaccttaac cttcatgccg tctttaaggc
    17581 tcttgactgt ccttgagctg aaggttctta caatctgctc gccggcttta aggcccgata
    17641 ccacctggta ctttgtttct tcggagagac ccagctcaac tttgcgctga tatatcctct
    17701 tgtcgtcctt gttgtagaca aagacatact tttctccgtc gattagcttc agcgtgtcaa
    17761 cggggattat tactgtgttc tccgcttttc ttgtattaat ctttatattc gcgttaaagc
    17821 cgctgattaa atcctcggtg ggatttttga cggcaacctt tgcctctaca tagctggagc
    17881 tgcccgtaag gccgccggta agggagccga tggcagaacc cagtcccaag ccgccttcct
    17941 ttgcggttgc gctgatgtag cttacctcgc cttcaaactc cttgcccatg tactcaacgg
    18001 tggcgggcat gccaacctta attgaggggg cgtcgtacat acccacttgg aatgtggcta
    18061 cgaacatatc gtagttttca accgttatgc cctttacatt cttcgtttcc gctcccgtga
    18121 gcatgaagga ggaaagaagg gagcttatgt ccgcgttgcc cgagagcata tcgagcagag
    18181 tgtttatgtc aatggagctc atgtcgggct gtgggctcgt gctttctatc ttctgaccct
    18241 tttggatgtt gatttcggag accttgccgc tgactgaagc gacccagccg gagttcagct
    18301 cattcagctt gttattcagc tcgttatact ctttctccgc tttgtccata acagccttaa
    18361 agctggcgac atagaggtcg cccgcctgga attcgtatat tgcctttgct gtttttagct
    18421 gcatgagctg ggaggcgtcg tcaagctgct gatactgctt gcctagaagc gcggagagac
    18481 cgcccgagcc gaggccgccg ttttgaaaca tctgaatcat gtcgttaatc tgctgaatgg
    18541 acgcgcccat ctgaataaga cttgcgagca cctgacctat ctggctctcg ccgaacagct
    18601 tggagatagc gtcaaccaca acggtattgt tgtttgtggg ggtggagctt cccgtatccg
    18661 cgccgctgtc tttgagctta tcttcaagct gagctatttt cgcctctacc gacggcagct
    18721 ccgctttagc cagagccgcg ctccttacag cctcctcgta agcctgtttc gcaaggtcat
    18781 aggctgcctt tttcttttgc acctcaagct gaagttctgt tgtgtcaaag cttgcaagca
    18841 catctcccgc cttgaccgta tcgccgacct ttacgaatat atcgtttacc accacgccgg
    18901 ggtaagcgta gaaaacgctc tgacccgatg aggtgagctg ggcagcggtt tcgtaaacct
    18961 cttgcacgga gccgattttg gcttccacaa gggttacata gacacgcttc ctttccacat
    19021 ttatgccgat aatgctgccg atggctatta agacggctaa tatggcagag acggttgcga
    19081 tgagcttttt gtcaagaggc tttttctcct ttttcggctt gggcgcgcgt acctcgtcat
    19141 agggcgccgt cagaatttct tcattttgtg ttttcttttg cttctttatt gaaaacttca
    19201 taatgtcacc gccatattat ttgcgcaaag tagattctct tattaaatat tgcataattt
    19261 acgcatttta tcaattattt tttacttttg gaaaattagg gccaccctta aaatggcctt
    19321 ttttgaatat actacccaga aataaagtat atagatgtat tatgttaaca ttctgtaaat
    19381 ttaaaagata tatagattaa catacgcagt gtccgcgcgg catatagttt aaaagacaag
    19441 ctcataaaaa ggtgaattga aatgataaag aaaataaccg ccgttttact ctcagtaata
    19501 atactctttc ttgccgcttg ctcccgccca aagagagtcg actctttgga gctttcaaaa
    19561 cgactggaga aaattaaaga atcttatgcc tttgacagcc ttgacactta catagtaaac
    19621 gatgagcata ttgtatttat ttctcttgtc aaagctaacg actgtcttct ttcctacaca
    19681 accgacggca ataattccat tacggaaatt aggctcacaa ttgacgctga gaactgggat
    19741 aagaatgacg ccgcttatat tttcggagaa tacgccgctg cggtgctgtc cgcgttttcg
    19801 ggctgcgctt atgacgaggc acttaactac gcaaaagaaa taggtctttt tgatgaaagc
    19861 gcctacttta atatgcacga agctgaaagc agttttgaca gatacagcat aaaaatgtac
    19921 gcaaaccccc tttgcgcggt cgcggaattc atatactcac cctcccttgt taagacttcc
    19981 gaagcctccg aaactgacgc ataagcgaaa ggtctttatg ccgacgccaa agctgttcac
    20041 agaaaattaa tacttccata aaccgagggc aaaagaatcg tcatttactt gtttgatttg
    20101 aataatatgg cataggcaaa aaaagcaata tgcacaaact tcgacgattt ccttaaaaac
    20161 tattgaattc aatattccca aatgatatat ttagcatagc agctttttaa agagctttgg
    20221 cgatgcaggc gtaatttaga ttttaaggag gctaattatg gatttcattc ttcagttcat
    20281 cgatttcatt aaggacattg ttaagaccat tcaggacttc atcaagaagg caagaggcta
    20341 ataaaaatag gctctgaagc ggaggtttga gcctccgctt ttttcagtat gaaaggttga
    20401 ggcaagatgg aaaacattag ctactcaaag gctatacccg taagatacga ggcggatgta
    20461 atggttgccg gttccggcat ggcgggcatc gccgcggctc ttggcgccgc catggagggt
    20521 atggatgtta taatcgttga aaggttcgga gtgctcggcg gcaatgccac agcaggcggc
    20581 gtcgcaaact tctgcggcaa caatgtgggt caaggctccg ttctggatat ggtgatggca
    20641 gcccttgagg agtttgacgc aatcgctccg agaaaaaaag gcaggggcgg cagagtcttt
    20701 gaccatgaga ttctggcggt aatactcccc gagctgcttg agagatacaa ggtaaaagtg
    20761 ctgctgcata caaccatttc ggatgtagtc ttaaacggca gaaggattga agctgcgata
    20821 gtcaccggtc cgtcggggct tgaggcaatt aaggcaaagc agtttattga ctgcaccgga
    20881 gagggcgcgc ttgccagaag cgcaggtttt tccgtcatga agggcggcag cagcaagtat
    20941 cagctcccca tgtcccttat gtactttgta aggcatgttg aggagaagga tgtaaaatac
    21001 attcccgaag gctggttcga gaaaatcgac agcgaggata agctccccat gaccagcatt
    21061 tggcccaacg gtccgaggag caacgcgata aaaattaaag tgccgatgta cgactccacc
    21121 gacactgttt ccatgacaat ggctgagatt aacggcagaa gaaagatgat gtcggtttta
    21181 gactatcacc aaaggcagga gaaaagaagc tggattctgg accactgctc aaccgtaatc
    21241 ggcataagag agggcgtcag gatagtgggc gactacatac tcaccgttga cgaccttaga
    21301 aactgcaagc gttttgagga tgttatcgcc gtcggcacat attatcttga cggtcacaag
    21361 cccgacgacg acaaaaggac atatatactc ccgaaagagt cattgaaggt tcctccctat
    21421 gacataccct taaggtgcct tatcgcaaag gacggggaca atctgatgat ggcgggcagg
    21481 tgcctttccg ccgaccagct ggcgctgtcc tccgcaaggg taatgaccac ctgctccatg
    21541 acgggtcagg cggcgggaat tacggcggct ttggcggtga agatgaatac ggatgtaagg
    21601 ggcgttccct atgccgaggt tcagaaaaag atgattgagg cgggcgctat ccttgacagg
    21661 gatgaggtag cctcattcta tgaagcggaa taaaaaatag cagcggcttt aatagccgct
    21721 gtttttttct taaagctcca tatcaagatt tttttcaatg aactttctta tctcgataaa
    21781 gtcctgcagc gctgcctctt tgttgttggg gtttctgagc agcgcggcgg ggtggaatgt
    21841 gcccataaag gcggtgccgc ttttcataac tatttgcccg tgctggcttg ttaccttgta
    21901 atcgggggag attagcctgc aggcggctat tctgccgagg cagaccacca gtttgggctt
    21961 tatgagcctg aactgctccc tgagccaggg tatgcactgc tcctgctcct caggcttcgg
    22021 atccctgttt tcgggcgggc ggcattttac tatgttggct atgtatacat tcttgtttct
    22081 gtcaagacct atggagttaa ggtacttgtc caggagcttg cctcccctgc ccacaaaagg
    22141 ctgacccgta aggtcctcgt tctctccggg gccctcgccc acaaacatta ttttggcctt
    22201 ttcgtttccc gtgccgaaaa ccacattggt gcgggtggcg tgaagcccgc atttagtgca
    22261 gccctggcag tcgtttttaa gctcctcaaa agatgtatac atttatttca cccttgcttt
    22321 tttctgatta cggttgaggc gatgtcgttt tcatcgtggg cggtggagat aatctccttt
    22381 accgactggc gggagtaaaa gccggccatc ttgccgccgt acaaatatat gcccgttaaa
    22441 ttgctgtact ttttaaactc ggggtttttc tttttgaagt ctatattgta cgatgcgtag
    22501 ggcattatga attcctgaag aatatagctg tcgtccttat gcttacggat aagctccgcc
    22561 cattcctcat cggagaaatg aattcccgcg taaactccct ttgctccgta ggagtcctcg
    22621 ggtttaataa tccatttgtc cttagccgtg aggacatcga gcttttctat aaactcgtcg
    22681 ctgagtttaa cggttttggg aacatgggcg tcaatgtagg cgatttcatc ctcggttaaa
    22741 aatgccttgg ttctgtcaag gtggaggatg tagaaaagaa ttttgttgtg gataatctgg
    22801 gtgcaaaagt cgccgatgag gacaactttt cttttcttta cagcctcaag gaagggctga
    22861 atctcgctct tgtgggtgaa aatatcggtg gtgacggctc tgcggtaaat ggcgtcaacc
    22921 ttgtgtcccg tgggggagat aagattttca ccgtcaaact taaagtccct tatttcgcaa
    22981 atctcagcct tacagcccgc tttttcaaat gcgagcttaa actgggcaaa ctcctcaatg
    23041 gagcagccct tttcaagaaa gtcggtgatg gcgatatagg gcttttcctt gggattttcg
    23101 cttgtattgt aaatctccaa aaaggttttt gcccatgagt cgaaaagctc aaaggaggaa
    23161 aactcgaatt tatcgcttaa atccttataa acctgagtga gttttatggc gatgttaagc
    23221 tccctgtcct cgttcattga gctgctgccg tcggcgttga actcgcagaa tttaaaggag
    23281 cggtcctcct cgttatagaa aatatccagc ctgcatatgg gaagaaggca ggagtaggat
    23341 ggtctgttta agattagttc ctccagctcc ttatcaaatc cgaaaagctc cctgtaatct
    23401 tccttttcga aatattcgtt tattacctta atgagaatgc ggtacatggt ttctatttta
    23461 tctctaaggt actccacatc ctcgtcggta aagagtttcg gcatgtaaag ggcgtagatg
    23521 gttctgccct tgtacttcgc ggtggagttt tcaaggtact cccacattct tttggagctt
    23581 tccatgttcc cttcaaagtt tttttcgatt gtatccttat aaaggaggtt aatatctttc
    23641 atattcataa taaagctcac ttctttcaaa caaggctgca gcactcttta aggcgggttg
    23701 ccgctgtttc cctcgggagc cttttttctt cgcagacgag tttcttaagg ggctttaaat
    23761 attctctttc gctggcattt aagtttttct cggcgacgga gaatactttt tttataatat
    23821 ctgccgccgt aatgcccgaa atttcagcgc tcagaccctt tttcatgatt tcgttttctg
    23881 cgtttttaat atcctcaagg gtgcttatcg ggagggtgtt aagatagctt tcgatgtcct
    23941 cggtattctc gaaaagtccc ttgattaagg cgacataaga aactgccaaa tccggcgaca
    24001 ggctgtcggc gtatcttatt tcaatatagg ttttaagcct taccgtgggg aaggacatgg
    24061 agaagatgtg cctaacctcc tcttgcgcag gctcttcgtc agcgtagatt tcctgcgcgc
    24121 tctttgtggt gcagcccgtt tccccgtact ccacgataag gggggaggcg gttacaaagt
    24181 cggcatagga gtcaaaagaa atgcctgcgt cacacgcaaa gccgcagtag tcggtgcggg
    24241 ctgtgtccac atcccgccat aatttcgtgc ggaggataaa ttccttattt tccgctccct
    24301 caaagacggg ggcgttgtcg cttaaaaggg ataggaaggg ggaaaggcgg caggcgattt
    24361 tatatttctt aatgaaatcc ttttcggagt aatagtctat ggaaacctgc gtcgccgcgg
    24421 tgcccttcat catgttcttg ccgtacctgc ctgttttggc aaagtggtta tacataaact
    24481 tgtagcgatc cttggggatg aggggcaaat cgtcaacctt tgaaacgggg tgatagccga
    24541 gatttataag tttcagtcca cgcttattca gcacagggtc gataaggcat ctaaagtctt
    24601 tatatatcct tacaatttcg gcgatgtcgg agcagggtct tatgcttatt tcaagctggg
    24661 cggcgggctc aagggtgatg gtatagtcgt catttccgag agcaaggaca aattcgtcct
    24721 ttctgactac cgtttcgaaa tggggagaaa gctcttccat gacagccgca acgccgtttt
    24781 cgccgaaata ctctattgac cttaaagtct tggcgtcgac taaaaagtgc tcaagctcca
    24841 accctaagga aaacttctca tattgacttt taatgccgcc ctctatatat gctttagcgg
    24901 cggcaatggc tttattctca ctcttgttca tactgttcct ttatttgcgt ttttggcttc
    24961 agccttcgct ttgcttcagc cttacataca tatgctttac tgttttgttg gatgtttccc
    25021 taacatctat gtcgaacata tcggtcgcga gaattaaggg agttagcttt gtgtagccgt
    25081 agtttctggg gtcaaaagag gggtttttct tgagaatcag attgcccaaa tcgccgagga
    25141 aggtccagcc gtcctcgccc gctatatcct caactgaggt gacaaggaga ttaataagtt
    25201 cctggggcag cggctctggg ctgagtttgc tctgggactg tcccctgccg gtagattttg
    25261 cgggagcctt ttctattttt acgggcttgc agacattggg ctttaatatc tcaaggaaaa
    25321 taaatttatc gcaggcggcg ataaagggct tcggggtttt ttcctcgccg atgcctataa
    25381 cttttttgcc cgcctctctg agccttgtgg caagccttgt aaagtcgctg tcgctggaca
    25441 cgatgcagaa gcagtccaga tttcccgtgt aaagcaaatc catggcgtca attataaggg
    25501 cggagtcggt ggagtttttg cccgttgtgt agctgtactg ctgaacggga ataatggcgt
    25561 tctccaatag gattgccttc caattggaga gattggggct tgtccagtcc ccgtatatgc
    25621 gcttaaccgt aggggtgccg tagcgggcaa gctcctcaag cattggcttt gcgtaggagc
    25681 aggagacatt gtccgcgtcg attaatacag ccatcctgga gtccggtttt tcactcattt
    25741 ttatcacatc tttttataca gattcttctt ttgcggtttc ttctgccgcg tgttcggaat
    25801 ttgaggtctt gaaaattttg attattccct tgtcgttaag gagcttaata agaattacga
    25861 taatgggcag aaggaaaata ccgatggggc cgaaaacctt agcgcctata tacattgaca
    25921 taatggttac cacaggcggc agacccacct ggtcggcaac cagtttcggc tctattatct
    25981 gccttattac tgttattata gcatacatta caagaagtcc gatgccgccg ccgaactcgc
    26041 ccgtaatgag aagatacgcg ctccacggta tgagtatggt accagtgccg agaacgggga
    26101 cgatgtccac aagggcggtg actatcgcga ttacaagtat atagtttacc tcataaatgc
    26161 ccgctacctt taaaagtccg agaccgattg agatttcgga gaaggttatg agcatgagaa
    26221 ggcagtacgc ctttgccatt ttgccgagag tgctgaacag cacggtttta gtttgggaga
    26281 gtttctttcc cttttcctcg ctgatttgcc tgtagataaa ctccttaatt tccttatagc
    26341 ttgttgccat aaagcaggag gaaacgacgg tgaccaccaa ggcaagcacg attgagggga
    26401 tttgctttgc ggtctcccat acgctgccga gaggtgattt gagccaggac aaatctaatg
    26461 agctgtcggg tacgccgggg actcttatgg attcggcgta ttttcttata aagttcgccg
    26521 ctgcctgctc aagggagtcg ggcaggaagg agataactct cagaagccag ttttccactt
    26581 cggtcacaat ggcaggtatg tcgtttaccc tttgagtgag atactgggca aaggatttca
    26641 gttcatttgt gagttttacg ccgatggcga acactatgaa aagcgcgatg gctgtaatga
    26701 gcagaaccaa agcagtaccc acaaaacccg gctttaagcg ggttaccttt gtgatttttt
    26761 caatcggctt ttgcagcgac atggtaagga agaacgcgaa aacaaagggg aagacatacc
    26821 agaaggcaat cttcataaag aggtagaacg ctgcgattat gataagccag aaaaacacat
    26881 cgattataaa ttttcgtctt ctttcggttc tgttcataat acctcatccc cggctcttaa
    26941 ataggcggct gtttatatta ttgacaggaa atattaacta aaaattaaaa tagtcgtcta
    27001 caattcttcc gtcgtgaatt cttatattcc ttttgattcc cgacgctatc tgattgtcgt
    27061 gggtaatgag gactatggtt ctgccctccg cgtttagctc gtgcagtatt ctcataacct
    27121 cgtcgcctgt ttttgagtca aggttgcccg tgggctcatc cgcaagaatg atgggcgggg
    27181 cggctgcaat tgcccttgct atggcgattc tctgctgctg accgcccgaa agctgtgagg
    27241 gcaggtgctt ttctctgtgg agcatgccca ctttttcgag ggcgacgcgg gacagctccc
    27301 gcctttcatc cgcgggcatg cctctgtata caaggggtag ctcaacattc tcccttgcgg
    27361 taagattgct tataaggtta aagccctgaa agataaagcc tatctccttg ttgcggattt
    27421 ccgaaaggct gtcgtcgtca agctcgtcca catatttgcc gtccagctta tatgtaccgc
    27481 tggtagggac atcgagcagg ccgagaatat tcataagcgt ggacttgccc gaacccgact
    27541 gaccgacaat gcccacaaac tcgccgaaat caatttcgag gcttacagcg tcaagggctc
    27601 tgacttcgtt ttcgcccgga ttgtaaatct tgcttatatt attcagctca ataaggctca
    27661 tattaagctc ctttaaaagc ggataatccg ttcctttgac attacttatg caataagcca
    27721 ccctaaaggc ggcttattgg tattttatgc gagaaaatgc ttcataagtt ccgcgccgct
    27781 gccaaagtaa agtcccgtag ctgcggctgt cttttccgta acggtgtcaa cgccctgctt
    27841 ttcattgttc ttgtggtaaa tcataaaggg tacggggtcg gtgctgtgtg tgcccgttgc
    27901 aaggggcgtg ggatggtcgg gaagtatcat tatcttataa tcatcaaatt ccttaagacc
    27961 ttcaaggact atgggcagga ctctcctgtc aatctcctct atcgctttga ccttgttgtg
    28021 gatttccctt ctgtggccgc actcgtcggt ggcctctata tgaatgtaaa caaaatcttt
    28081 gccgttttta agctcatcta ccgcggtttg tgccttgccc tcaaagtttg tgtcaaggta
    28141 gcctgtggcg ccctcaacgg tgggggtgga catacccgca agaccgccaa tgccctttaa
    28201 gaggtcgacg gctgagatta tgctgccatc aagaccgtag aggtccttaa agagggggag
    28261 attgggcttt gtgccttcgc cccagagcca gattgagttg gcggggcgct tgccctcggc
    28321 gattctcttt ttgtttacgg ggtggtcttt aagtatattg tagctttcct tcatcattct
    28381 gatgaggtcc gccgcgtttt cgctttttga aagataggga cctatgactc tgtcggaaat
    28441 gtcgtgggga ggagtcattc tgccaaggtc caccgtgccg ccgtgccata caaggcagtg
    28501 cctgtacgcg acgccgctgt aatactcgta aatgtcgctg ccgaaatgct cctggatagc
    28561 gtctatcagc agttttgcct cggctgtgga aatatcaccg ccggagtagt ctatcattgt
    28621 cttgtcctcg taattttcct catcggaaag ggtgacaagg ttgcagcgca gtgtaacatc
    28681 agtatcgctg aggttaactc cgatgctgac agcttcaagg ggtgagcgtc ctgtgtagct
    28741 ctgtctcggg tcgtagccga gaacgcttaa attggcaaca tcgctgccgg ggttcatgcc
    28801 atcgggaatt gttttgacga gacctgcctc gccctttttt cccaaagaat caaagagagg
    28861 cttgtttgcc gcctccatgg gcgttttgcc gccaagctcc gctaccggca tgtctgccat
    28921 cccgtcataa agtacaacta catatttcat ctatataaaa cttcctttca cataaggggc
    28981 ttgccccttt gccacatcca tcggctaaag gcgcaatata atctattctt caacaggcaa
    29041 gggtgtgtcg ggatttatct cgcctgattt tactctgtca aaataagtac tggttgaagg
    29101 gataacgaat tttacgctct tgggaacgat ttcaaaggtg ctctcggtca caatttcgac
    29161 ttcgccgtca atgttgaccg cggcgggctt ttcgctcttt acggtgattt ttttgcccct
    29221 tttgaactcg gtaaagggca gactcagatg ctgacccttc ttgtattggt cgacaagacc
    29281 cagcatctta gcccttgaaa cctctttcct gactatgacg aaatcaagaa gaccgtcgtc
    29341 ggggatggca aggggggctc ccataaagcc gccgccgtac catctggaat tggcgcagag
    29401 gcagaacaga acggtgagtt tcctctcctc ctcgccttct gtttgtatgg tgaactcgtt
    29461 cttgattttt ctgaggaagc agtataaaag agccgcgtaa tacgcgcttt ccgccttcat
    29521 gcccggcagc tttttaaagc tggcctgctt tgcgcagatt tccgcgtcaa ggcccatgga
    29581 gcactggtta acggcaatct tgtcgccgca tctgatggcg tcaattacaa ggggaatacc
    29641 gttaatctgg gcgttaaggt ccagaaactc ttccttttct
//
LOCUS       DAONVQ010000007        25865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_1268_length_25865_cov_4.704843, whole genome shotgun sequence.
ACCESSION   DAONVQ010000007 DAONVQ010000000
VERSION     DAONVQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 25865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25865
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1268_length_25865_cov_4.704843"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    complement(149..382)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            620..696
                     /locus_tag="PLD98_01120"
     tRNA            620..696
                     /locus_tag="PLD98_01120"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:654..656,aa:Met,seq:cat)
     gene            798..2147
                     /locus_tag="PLD98_01125"
     CDS             798..2147
                     /locus_tag="PLD98_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA04888.1"
                     /translation="MEFIVEPERKVKICDEADVCVVGGSCTGVFAAVRAARLGAKVAL
                     IEKQNCLGGTATNGLINIWHSLYDTDGKNQIIAGLTLETLERLKKAGAVWRGESVTGA
                     YHFNSQELKIELDNYIRENKIRLYLHTYYAGLLHDGNKIKAVIIENKDGRSAVKAGFF
                     VDATGDGDLCRDLNIESYQNKTVQPPSSCFLMKGDIDSDLLSQIIRKHGHEEGLEDDW
                     GWSDRSVCGDDITLRADNHVFNVICNKARDLTFAEMEGRRQMRAFINLLKKYGHPDMD
                     YSLVAACSHIGIRETAHYKTLFKATEKDLLMGKRYPTAVLNGTYRVDIHHSDNMGITF
                     KYLDGTVSTFYGKNTKTEFGNWRTREGITGEPAKYYQLPLEVLIQDRYENFIMAGRML
                     NADEGAFGALRVMVNLNQLGEAAGVAAFETLNSGKPVWEIDRIKVRRTLKDGGSALI"
     gene            2453..3220
                     /locus_tag="PLD98_01130"
     CDS             2453..3220
                     /locus_tag="PLD98_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQA04889.1"
                     /translation="MAPKSIKGSPVLAKIIKARRLELGLTIEEAAFKAGVGTKTWSRY
                     ESGESIRADKYKGVCKALEWKKLPDIEKDYEKDYSNILDFDQYRTHEAWSKYIEDSFG
                     EAAAATFVVGSDILLDEIQEDMNELARMPKGTHIGQLNNSWLESLLPPQFLMEYDYNF
                     LYLLRYNVERLRKIAHHGGQIIAHSVLDELTLYLIVEESRSLFEDEYGLDDYIFDWVF
                     DLFEDMDIITFLYSDLFYLSDEHAYHFNQWNINQFFT"
     gene            complement(3373..6732)
                     /locus_tag="PLD98_01135"
     CDS             complement(3373..6732)
                     /locus_tag="PLD98_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04890.1"
                     /translation="MLPSGSIQAAIDAADAGDVIFVISGTYKERLTVNKPLTIWGLNY
                     DTDGDGDRIYEAFVTYPDGLDTSDLTLLNVSADNVVIKGLTFINEQETAGSAKELSFT
                     GENIEFANNRVIVKANKTALYFGSDSTAPNARGGVVVRANYIEAAGTALHIQGIGAVV
                     EENEITGGTALKVEPKANSAGGSVQGNALNAFQNGIVHSSALSGSGQWKYDDNTVTAA
                     AAAGVSGVWTGISLSSAEASAVFTGNTVDGTTAVNSGAFDEVIGIHFAGVISGAAAFA
                     FSGNAVSGVSLGALKESGSADLNAIFADNTFPEGSMVIGNEIKVPTWADFADTSWYNA
                     ALSEYDISTAEQLAGLSALVASGINFNGKTLNLINDIDLQNIEWIPIGDGSRWFGGIF
                     NGNGYKITGLKITDNTLANVGLFGYVRGAGDTAGIRNLTVSGKIDIAGSKVFSPNVGI
                     LAGLVWGFVENCHTTSVKEENTISFKSECGDSGQHRVALGGLVGWNANSNYPHNGMAD
                     SSFTGTINVETETPYVPGVGGNSGAYVGGLLGMANRVNVVDSFAHADITANAYRYLSM
                     GGIMGYSMGEKTRFENLEFDGNLNGCTDMTGDSNYSTCSVQIGGILGSGEFAGASAPE
                     SGFVNCTAKGALNADIDGEGASVYTGGIVGYVYPYWREPYLAYFGIEGCEVDLLHNVD
                     LDTPANIYTGGLAGIIYNDASAKTTISLADSEVKGAINVSNTAAGTGKRAYIGGLFGY
                     GLKNNTDPALNAVFSLNNCTAVAPTVSLDYDNLKASDMWGKIEWAEAAVNNVYYSSWL
                     DAVSALQEGGTFTFTAEQVTIDRKVMFRKNNITIRGIADINGKPMTTIKGVGNSVEQF
                     RIEGYNVTLRDLNFDSIANGGGARENNGVYFYYPNNIWAADRVNYLINCHSTNTYWAI
                     FSGIKLNAVIEDCTFENTNNSAKIYMDRPYDVTIRRSFFKNDDSKFSLHIDNGTVGGN
                     VTVEDCVFVSYFTTAGDVANTTGAGNITYTFRNCDFYTNSGVEGSWCSVWYANDTRFI
                     GCRFHEGFEGIGAYPYEGGSVFVDSGCAFVGGPVDISIYYGVGAGVLAGVNVQLDSEG
                     KVIGGTFKSDPTAYLADGYTATLSGGVWIVG"
     gene            complement(6756..7220)
                     /locus_tag="PLD98_01140"
     CDS             complement(6756..7220)
                     /locus_tag="PLD98_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQA04891.1"
                     /translation="MKYLSKTLALVLVFLLAFSSFNLVAFAAAAEDVTALEAEEDYIP
                     GFLERLKGLVDLIRNFIDSIKNFVFGLLGIVKPIPVRSVGLNKLFLLLQVGESEQLIA
                     TVYPDVATNKDVIWSSSDESIAVVDQNGLVTAAGSGIASITVTRLTDAKQHP"
     gene            complement(7646..8467)
                     /locus_tag="PLD98_01145"
     CDS             complement(7646..8467)
                     /locus_tag="PLD98_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HQA04892.1"
                     /translation="MNVDVDLPVSLNLRIMRKYDDLTSAEKKVADVVLNLGDSIIEHN
                     IKSLAEESKVSEPTVVRFCRSIGLSGIKELKKAAMQSTVAETPAPPSSHISTLESKDQ
                     LVAFVLDNMAYILGETKKIVNEESLFKAIDLINKASFIKILGYGGSSIVARHIHHYLR
                     MAGKHVELLRSQEDYYPSLREQYDKSDGGLAISYSGNTEVILDLVRDAKAKGARIISI
                     TSWGECKLNSLADVSLQSYFYGDGIIPGVHAFERISQLAIANMLITGLYKLNQPT"
     gene            complement(8565..9506)
                     /locus_tag="PLD98_01150"
     CDS             complement(8565..9506)
                     /locus_tag="PLD98_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQA04893.1"
                     /translation="MKLDKLRATRQGYGDGLAELMREKSDVIALDADLAMATGSSAVK
                     KEFPDRFVDVGIAEQNLVGMAVGLSLTGYVPYASTFAVFMAGRAGEIIRNAAAYSKAN
                     VKFVGSHSGITPVGDGGTHQCIEDFGSMRAIPNMTILSPCDYNQAKVLVKKAYDIKGP
                     VYIRTSREGVPEITDENQDITIGKAQILKDGEDICIISTGFPVSLVLEALEELEKDNI
                     NAAVLNIHTIKPLDKETILEYADKCKKVLVVEEHNVLGGLSEAVAAVLVGKDGISFDR
                     IGIEDRFGQSGSKAEVLNEYGLTSENIAKRARNLLAK"
     gene            complement(9503..10312)
                     /locus_tag="PLD98_01155"
     CDS             complement(9503..10312)
                     /locus_tag="PLD98_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009167918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQA04894.1"
                     /translation="MSYDLYKEKAAFIRRTVLDMLESAGSGHPGGSFSATDILTVLYY
                     GKMRLFDDPRDDRRDRFILSKGHANPPLYAILADKGYIEKEELKRLRHFGNKLQGHPD
                     MNKCPGIDCSTGSLGQGLSVAVGMALGFKKQNKDNHVYVLTGDGELQEGIVWEALMAA
                     AHYKLDNLTMIADCNGLQLDGSTDEVMSLGNLADKVRAFGFEVAEIDGHNFEEITKAL
                     DIKPQGKPLCIIARTIKGKGVSYMENNFAWHGKVPQGELLEKAKAELGGRV"
     gene            complement(10328..11194)
                     /gene="fba"
                     /locus_tag="PLD98_01160"
     CDS             complement(10328..11194)
                     /gene="fba"
                     /locus_tag="PLD98_01160"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HQA04895.1"
                     /translation="MPYVSAKEMIQDAKNGKYAVGHFNLNNMESIRAYLLAAEEMKSP
                     VILGVSGGTAKYMGGYKTIVATVTSFIDYLKISVPVALHVDHGTYEEVLDAIESGFSS
                     VMFDGSALPIEENIEKTRYILGLCREKGISLECEVGAIGGEEDGVAKMGEYADPNECA
                     KMAELGIDMLAAGIGNIHGKYPKDWPGLRFDILEEIQKATNGIPLVLHGGSEIPTDMI
                     KKAISMGVCKINVNTECQIAFTQAVREYIEEGRDLVGKGFTPRALLAPGLEASKQVAI
                     EKMQLFGSVNKA"
     gene            complement(11215..12867)
                     /locus_tag="PLD98_01165"
     CDS             complement(11215..12867)
                     /locus_tag="PLD98_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQA04896.1"
                     /translation="MTKSRKRPLELWHLISIAITLLYLLFLIVPMFNILKESVYENGR
                     FTLKYFIQFFSEPYYFNTILNSFKISIATTLFSLLVGVPLAYLYNLYRIHGRSVVQVL
                     IILSSMSAPFVGAYAWILLLGRYGLITKFFAELGIALPSIYGFRGVVLVLTTRLYPLV
                     FMYVSGALRNVDNSLLEASQNLGVSGVKRFFKVVVPLCTPSILAAALMVFMRAMADFG
                     TPLLIGEGYRTFPVEIYKQYVGETSQNYNFAAAISVIAIVITAIVFLVQKVASEKFSF
                     KMNALHSIERKKAKPVTSFFIHAYTYIVVAISMLPQVYLVYCSFRNTNAFGNFTKGLS
                     LNSYKNAWKYVRTALPNTLIIGLSTMVIVVLMSILIAYMVVRRKNTMNNVLDTMSMIP
                     YIIPGSVIGIALVLTFSKGWLVLTGTMAIMIIAMVIRRIPYTIRSSVAVLQQIPITTE
                     EASISLGASKLKTFFKVTTPMMASGIVSGAILSWVSIITELSASIMLYSARTQTLTLS
                     IYVLVSRGTDGAACAVSTILLLFTVISLALFMRVSKNKDIVM"
     gene            complement(12880..13965)
                     /locus_tag="PLD98_01170"
     CDS             complement(12880..13965)
                     /locus_tag="PLD98_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA04897.1"
                     /translation="MSINIIIKNAVKRYGDVVIIPDLSLDIKKGEFFTLLGPSGCGKT
                     TLLRMIAGFNTIEGGDFYFNETRINDLNPAKRNIGMVFQNYAVFPHMTVRGNVAFGLK
                     NRKMSKEEIKKRTDEFLELMKIKEYENRLPERLSGGQQQRVALARALAVHPDVLLMDE
                     PLSNLDAKLRVEMREVIKEIQNKIGITTVYVTHDQEEAMAISDRIAVMDKGVIQQIGT
                     PKVLYHRPANLFVATFIGKTNVLDGKIYEENGEKGVMLENAKFPMKNLNDLAVDGLNV
                     KLSIRPEEFVICEHGCGSLKGIVEDSVFLGLNTQYFVRLESGEKVQITRESSIEDTIE
                     KNSEVEISVKTDKINVFSEDGQRALIG"
     gene            complement(14090..15169)
                     /locus_tag="PLD98_01175"
     CDS             complement(14090..15169)
                     /locus_tag="PLD98_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQA04898.1"
                     /translation="MKKFVALLLVFCTVLALFGCKPADKDTTDKGTSAPASGPDESVK
                     GKLVIYTTTYDIEYKLIIEEGFQKKYPNVQIETVQAGAGELKTRIKSEIENPQGDVMF
                     GGLAYSDADLGIWEKYVSKYDSTMPESMRKTTGYLSWTTIQLENLLVNIDAAKKAGID
                     PDSITGFESLLNPALKGKIIWADPASSSSAWNMLATMLVAMGGYESEKAWDYVDKLLA
                     NGVVVGNSSSNCYKSVYEGEYVVGLTYEAPCVSYIEGGEGDKVKIVYMEEGTSAFPFA
                     SAIIKGAKNMEIAKLFIDYLVSEEAQKLWASSTARQANTKLPTTNQYLTDISKIKLVE
                     SDHQYLKDNRDKILEKFQQIYVKYN"
     gene            complement(15194..16141)
                     /locus_tag="PLD98_01180"
     CDS             complement(15194..16141)
                     /locus_tag="PLD98_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQA04899.1"
                     /translation="MIRTKGGVGIVSLVGVGHCCHDYLCLIDEYPPEDGSTRITSVST
                     QGGGAVATAMVAASRLGIESAFIGNIGDDEVGSLILEELKREGVDVSGVEVLPGVQSL
                     RSYVMIDTKKGTRTKFPYKGNIPPIVWDEHKKNMLKNAKILHLDGTDYENAVNAAELA
                     KEYKVLISLDGSTRQKDNRLNKELASRVDILIMSAVYPTAVSGKPTIKEALLEISTWG
                     PEIVMSTAGADGVFAVIDNHVYHFEGYKVNVVDTTGAGDVFHGAFLAAYLKGKNLEDC
                     ISIAQLTAALKCEHIGGRTGITDWKTVENNYKPFAGVRL"
     gene            16511..17623
                     /locus_tag="PLD98_01185"
     CDS             16511..17623
                     /locus_tag="PLD98_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HQA04900.1"
                     /translation="MSKNYDIAAYIWTAYTGDEPRARIFWKEGIGEWQSVKNAVKKFP
                     EHSWPRKPLWGYVNEADPEVMEMEIDCAAKYGVNVFIYDWYWYDDRPFLENCLNDGYL
                     KAKNNHLVKFYIMWANHDANNLWNIDLSDDFGDVVIWKGAVGFDSFKVIVKRLIEKYF
                     THPSYYKIDGKPVFMIYDFKNFVSGLGGSEGAKRGIEYFRNEAKKAGFPDLHLQLAAW
                     GKHNLNHSGVDSNGGADSCDYKDLGFDSITNYQFVHFTVPNRPYPQILEDVKREYERI
                     DRTYDIPYFPHVSIGWDNNPRYKRLRKDIITENTPENFEKALRMAKDYADTHDLPAKL
                     ITINSWNEWTETSYLQPDDLYGYGYLEAIKKVFKDE"
     gene            complement(17659..18474)
                     /locus_tag="PLD98_01190"
     CDS             complement(17659..18474)
                     /locus_tag="PLD98_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04901.1"
                     /translation="MSFKLGFNADIPNIEPKVEAYVPPKETKPRKSLVRVYFESLDRA
                     YTYYNDRFDLKLGDRVYVEGKLEGNQGRVVEINCNFKIDLADYKRVIAVIDTDIKGQF
                     YIAGSHILTFDPNALPIEKASLWFMPPIAEDAEIVSGYDDTSFRLDELSGMNVGGDET
                     KRGFDYYFENKVRYICLDKTKGYAVVQGSKVYEVEFEYQNGEVSKLTCSCFSCLNCRH
                     EFAAMLQLNDTLEQIRKLNAAEFERSGYFAAADKEILVKYGIMARNSGGIKFE"
     gene            complement(18488..19054)
                     /locus_tag="PLD98_01195"
     CDS             complement(18488..19054)
                     /locus_tag="PLD98_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04902.1"
                     /translation="MARKGRSKRDLFGAIHHYDENGRKIGKSEPSIIGGYINYDRQGR
                     KIGYSSPKIFGGYTHYDKRGRIIGKSESKNFKGYVHKDAKGRMVSTSDHGLFGGYQHK
                     NTKEGCYIATCVYGSYESPQVMTLRRFRDNTLSKTFLGRLFIRAYYSLSPALVKRFGG
                     NKSFRAFFKALLDRVVNRLNSQNSPKFR"
     gene            19273..19941
                     /locus_tag="PLD98_01200"
     CDS             19273..19941
                     /locus_tag="PLD98_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04903.1"
                     /translation="MVENSVFCNQCGYSFMDGKAATNKWYENKAVVLLLIFLFYPAGF
                     YLLWKMNPKKSVRAVIALAVIFTTLFWLSIIGFIGSELDASTTFDNSINTSVAEEETP
                     DAESAKKEYVAACKTYSYKEILRNPANYEGEKAYFQGKVIQAQESGGKTVLRVNVTKG
                     ENNAWTDTIYVEYTKKDENESRILKDDIIKIYGELDGIKNITASGQKTNVPKIAAEYI
                     DIVE"
     gene            complement(19995..21056)
                     /locus_tag="PLD98_01205"
     CDS             complement(19995..21056)
                     /locus_tag="PLD98_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQA04904.1"
                     /translation="MLSWVDLTEIIKALIDENIEQGITLSQISEHCGYSPHYLSKKFH
                     EIEGISLKEYILISKIQHSAAALLNTNHRIIDIAVSHGYSSQEAFTRAFIKVYGITPW
                     GYRKLQKPSPAAEKSRLIPLAGVKQQIGGLNMKIYVKQMYDWNHYAFIAEDVEEKHWD
                     YFTENQWWQVGNNFIKSYDNVRDFEYCAKNFTKYGETAVKQQLKLIETPWQKALESFC
                     SEIQKLGVDWYVHGSAAMALWGIDVEPKDVNIIVANYSDFDKVRDHFRKFAIRPIERC
                     ENWVMSGLGEIFMDAVISIAFHNKELEPYDMSTLGKVNFNGKEIYISSLEMLKEDNIN
                     FNRPDRVKAIEEKIKELGK"
     gene            complement(21181..21257)
                     /locus_tag="PLD98_01210"
     tRNA            complement(21181..21257)
                     /locus_tag="PLD98_01210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21221..21223),aa:Arg,seq:ccg)
     gene            complement(21338..22432)
                     /gene="ychF"
                     /locus_tag="PLD98_01215"
     CDS             complement(21338..22432)
                     /gene="ychF"
                     /locus_tag="PLD98_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HQA04905.1"
                     /translation="MKLGIVGLPNVGKSTLFNALTNAGAQSANYAFCTIEPNVGIVPV
                     PDERLTRLAEMYNPHKVTPATIEFVDIAGLVKGASKGEGLGNKFLSHIREVDAVIHVV
                     RCFEDDNIIHVDGSVDPQRDIETINLELILSDAEILDRRIDRDTKALKGDKSVAKSLE
                     VFKKVRAELDNGVCARAIPLTDEEKEILSTVPLLTAKPVIYACNMSEQDFADNLENNK
                     YYKAVEKIAEAEGSQVLPICAEFEAQLADLDKEEREIFLNDAGIKSGGLDLLIQKSYA
                     LLGLISFLTAGEQEVRAWTIKKGTKAPQAAGKIHSDFERGFIRAEVISYEELIECGSM
                     AAAKEKGLVRLEGKDYVMQDGDVVLFRFNV"
     gene            complement(22479..22772)
                     /locus_tag="PLD98_01220"
     CDS             complement(22479..22772)
                     /locus_tag="PLD98_01220"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQA04906.1"
                     /translation="MEVLTKKIDKDEADKKLNFATWGTWGCGAETFDWSYPDSSETCY
                     ILKGAADIHYEGKTTHIEAGMLVHFPKGLECTWAVSEPIQKYYKFGLNVDGLF"
     gene            complement(22824..>25865)
                     /locus_tag="PLD98_01225"
     CDS             complement(22824..>25865)
                     /locus_tag="PLD98_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HQA04907.1"
                     /translation="EECLEPYIFTKVGEVDFKAFVTDKQYHEIPDDSLFEPIEKGFTW
                     QGENRYCWYKGEYTVPAELEGKPLFIKPKMEGYEAMLWVDGRVSGTFATKIVYTGHGN
                     HYCDMIKMNPKAGEKINIAIEYYGGHHCIGCQPFEVAPIKNYNFKYEGADICVKNYEI
                     QDFYFDLQALNQMAEALPDDSFRKGEIINCLYEVHNNVYYSIDNVDWDTFMESLRKVA
                     PLMKQVLSVKNPESAPEAGIIGHSHMDTAWLWHIGETIKKCARTYSNALSLMEQYPEF
                     TFIQSSACHSNMILKHYPELFERIKEKVKEGRYEPNGGVWVECDCNITSGESIVRQFL
                     WGQRFNRKHFGYTSNCFWLPDTFGYSAAIPQIMKGCSVDYFLTTKISWNDTNVFPYDT
                     FYWQGIDGTKVFSHFNTTHHFPNVEDVFDRVNKGVKQKSVTNKRLLAYGFGDGGGGPQ
                     FEMVEQARRIKDTLGVPKTQHVRVGDFMKELEESVKNPNVYKGELYLELHRGTLTNQH
                     TIKRNNRKAELALRDLEILTVANAVTKGEEAKNDEINPLYEELLINQFHDILPGTCIT
                     RAHEESREKMGQVIKTAKELIAKTVDSEGEDYVTLINTLSFDRSDAVFIDRKPGCIIE
                     GEYAQQVYTDLDGNEKLILAGLTIPAFGSVTLKWVKGEPGGRSAFEAFENKLETPFAD
                     VTFSENGAIESLYDPKAHREIVGGLPLNTFLMAEDVPSAWDNWDVDADIESKFKPSME
                     LISREVVSDGEVAYIVRSEYRISEKSTIKQDMIFFANSAEIRFDTAMDWNDDHRFLKA
                     AFETTITEDFARNEIQFGHVKRPTTRNNSLEQAKFEVVNHKYTDLSETKYGVSVLNDC
                     KYGITLNGSGMYLSLHKGGTRPDEKGDKDGIHRCTYALLPHNCGFSSESVIHPAYMLN
                     VPPVQANGKYSAKQLMTVVSPNIIVEAVKPCEDCERAFIVRFYEAEGTFTNTRAYFCE
                     GIDKAYETNMLEEVQKELEVWANGIEMTFKPFEIKTIKVTY"
BASE COUNT         6349 a         6074 c         6128 g         7314 t
ORIGIN      
        1 tgctctgccg cggcccttac cagctttaaa tgccctatat ggggaggatt aaagctgccc
       61 ccgaaaatac ctactctttt catcctgacc tccgtttgga gcttgttttc actcccataa
      121 aaacaaaaac ccttaacgca caagtgcttt aaggggcgaa tttctccgcg gtaccaccct
      181 gattcggcat ataaatgccc tcagacccgc cgataacggc agaggttcga catataacgc
      241 tgtcagccgt cctgccctac tcattgttat ttcggacagg caactccgga acgagctatg
      301 cacacaagcc cccgccgctt tgcaccaacc agcggctctc tgaaagcaaa gccagtgtct
      361 ttctttcctt cacagtcttt aaatatctaa tttgcaaaat cttcctatgt gttaaatgat
      421 tatacatcaa agaaaagact aaagtcaaga aggtgaatat tctttgcttt tttgtcttac
      481 aattaattat gtcctgaatt tagcttctta atagttttaa caccgagtcg caaacgggaa
      541 taaaatggaa gcaaaaacac gcaaaaaggg tcttgatttt attggacttt tattgtatat
      601 tatataggca atcagatgac gcggagtaga gcagtccggt agctcgtcgg gctcataacc
      661 cggaggtcag aggttcaaat cctctctccg caaccagtta aagctaagaa tcaatccgat
      721 tcttagcttt tttctttttg tcaagcggta gcttttcgct ctgaaataat ataagatcat
      781 ataaaggggg cgggcagatg gaatttattg tggagcctga aagaaaggtt aagatttgcg
      841 acgaggcgga tgtatgcgtt gtgggcggaa gctgcacagg cgtttttgcg gcggtcaggg
      901 cggcaaggct gggggctaaa gttgcgctga ttgaaaagca gaactgcctt ggaggaacgg
      961 cgacaaacgg ccttatcaat atctggcaca gtctttacga tacggacgga aaaaaccaaa
     1021 tcatagcagg gctcactttg gaaactcttg agcgtttaaa aaaggcgggc gcggtttggc
     1081 gcggggaatc ggtcaccgga gcttatcact ttaattctca ggagcttaaa atcgagcttg
     1141 acaactatat tagagaaaac aaaataaggc tttatcttca tacatattac gctggtctcc
     1201 tgcatgacgg aaataaaatc aaagccgtaa taatagaaaa caaggacggc agaagcgccg
     1261 taaaagctgg ttttttcgtt gacgccacag gcgacggtga cctatgcagg gatttgaata
     1321 ttgagtcgta ccaaaacaaa acggttcagc ccccaagctc gtgttttttg atgaagggcg
     1381 atattgactc ggatttactc agccaaataa tcaggaaaca tgggcatgag gagggtctgg
     1441 aagatgactg gggctggagc gacaggtccg tttgcggcga tgatattacg cttagggcgg
     1501 ataatcatgt atttaatgtt atctgcaata aggcaagaga ccttaccttt gctgaaatgg
     1561 aaggcagaag gcaaatgaga gcctttatta atctccttaa aaaatacggt catcccgata
     1621 tggactactc tttggttgcc gcctgctcgc atataggcat cagggaaacg gcgcattata
     1681 aaactttgtt taaggcgaca gaaaaagacc tccttatggg caagcgctat cccacagccg
     1741 ttttaaacgg cacctaccgt gtggacattc accacagcga caacatggga attaccttta
     1801 agtatctgga cggcaccgtc agtacctttt acggcaagaa tacaaagacg gaatttggaa
     1861 actggaggac tcgggaaggc attacgggag aacccgcaaa atactatcag ctcccccttg
     1921 aggttttaat acaggacaga tacgaaaact ttattatggc gggacggatg ctgaacgcgg
     1981 atgagggcgc tttcggcgct ctgcgtgtga tggtgaactt aaatcagttg ggagaggcgg
     2041 cgggagtcgc ggcctttgaa actttaaaca gcggcaagcc tgtttgggaa attgatagaa
     2101 taaaagtgcg aaggacatta aaagacggcg gctcggcatt aatctaagcg atagagtttg
     2161 ttttcttttt ccaaagcccc tcggacaaaa aaaccacagc ttttgggctg tggttttttg
     2221 taatcttctt actagtctta taattttgca gaagacggtt tttcaatatt taaatagggc
     2281 ttcaaatcac ataaaaaact ttatgattgt tatgtgcaga atgactcctt tcttttcatc
     2341 tgtcatttgt tgtcgaaata tgtatcacga atataatgtt taagctcttg acaaccggac
     2401 acaaaatgtt ataccgacat tgatgtgacc tgtcataaag gaggtaagaa ttatggcacc
     2461 caaatccata aaaggcagtc cagtattagc aaaaatcatt aaggcaagaa gacttgagtt
     2521 aggacttacg attgaagaag ctgcttttaa agcaggcgtc ggcacaaaaa catggagccg
     2581 ttatgaatcg ggtgaatcta taagagcgga caagtataaa ggagtttgca aagcattaga
     2641 gtggaaaaag ctacctgata ttgaaaaaga ctatgaaaaa gattattcaa atattctgga
     2701 ttttgaccaa tacagaacac atgaggcatg gtctaagtat attgaggatt cgttcggcga
     2761 agctgctgcc gcaacatttg tcgtaggcag tgatattttg cttgatgaga ttcaagagga
     2821 catgaatgag cttgcgcgaa tgccaaaggg aacacacatt gggcagttaa acaattcgtg
     2881 gttagaaagt ttattgccgc cacaattcct tatggagtat gactacaatt ttctttatct
     2941 ccttaggtat aatgtagaaa gattaaggaa gatagcacat cacggcggac agataattgc
     3001 ccatagtgtt cttgatgaat taactcttta tttaatagtt gaagaatcac ggtctttatt
     3061 tgaggatgaa tatggtttag atgattatat ctttgattgg gtttttgact tatttgaaga
     3121 tatggatatt attacttttt tatattcaga tttattctac ctgtctgatg aacatgcgta
     3181 tcacttcaat cagtggaata ttaatcaatt ctttacctaa caaagtgaca ttaaataatc
     3241 gcttttaatt ctgggagcat taccttttag attagtcaga gcttaaaatg tggtcttttt
     3301 aaaaaagcag aacactgccc cttaggacag tgctccttta tttggctaag cgacttggct
     3361 tcgtatttcc agttagccta caatccaaac tccgccgctg agtgttgctg tgtagccgtc
     3421 tgcgaggtag gctgtcgggt cggacttgaa tgtaccgccg ataactttgc cctcgctgtc
     3481 aagctgaaca tttacgcctg ccagtacgcc ggcgccgact ccgtagtaga tggatatgtc
     3541 aacaggtccg cccacgaatg cgcagccgct gtctacaaat accgagccgc cttcatacgg
     3601 ataggctccg ataccctcaa atccttcgtg gaatctgcag cctatgaatc tggtatcgtt
     3661 ggcgtaccag acggagcacc agcttccctc aacaccgctg ttggtgtaga agtcgcagtt
     3721 tctgaatgtg taggtaatgt tgcctgcgcc cgttgtgttg gccacatcgc ctgctgtggt
     3781 gaagtagctt acaaatacgc agtcctctac tgtgacattg ccgcctacgg tgccgttatc
     3841 gatgtgcagc gagaacttgc tgtcatcgtt cttgaagaat gaccttctga tggtgacatc
     3901 gtagggcctg tccatataga ttttcgcgct gttgtttgtg ttctcaaatg tgcagtcctc
     3961 tattaccgcg ttgagcttaa ttccgctgaa aattgcccag taggtgttgg tggagtggca
     4021 gttaatcagg tagtttactc tgtcggcagc ccagatattg ttgggataat agaagtatac
     4081 gccgttgttc tccctggcac cgccgccgtt tgctatgctg tcgaagttca ggtctctgag
     4141 cgttacattg tagccctcga ttctgaactg ctctacggag ttgccgacgc ccttgattgt
     4201 ggtcatgggc ttgccgttta tatcggcaat gccgcggatt gtgatgttgt tctttctgaa
     4261 cattaccttt ctgtctattg ttacctgttc agcggtgaat gtgaaggtgc cgccttcctg
     4321 cagagcggaa accgcgtcaa gccaggaact gtagtataca ttgtttacgg ctgcctctgc
     4381 ccactcaatc ttgccccaca tatcgcttgc cttgaggttg tcgtagtcaa ggcttaccgt
     4441 gggggctact gctgtgcagt tattgaggct aaatactgcg ttaagggcgg ggtcggtgtt
     4501 gttcttaaga ccatagccga acagaccgcc gatatatgct ctcttgcctg tgcctgcggc
     4561 ggtattggag acattgattg cgcccttgac ctcggagtcc gccagagaaa tggttgtctt
     4621 tgcggaagcg tcgttgtaga taatgcccgc aagaccgcct gtgtagatgt tggcgggagt
     4681 gtcaaggtcc acattgtgca ggaggtcaac ctcgcagcct tcaattccga agtaagcgag
     4741 ataaggctct ctccaatacg gatatacata gcctacaatg ccgcctgtgt agacggatgc
     4801 gccctcgccg tcgatgtcgg cattcagagc gccctttgct gtgcagttta caaagccgct
     4861 ctctggagct gaagcgcctg caaactcgcc cgaacctaag atgccgccta tctgaacgga
     4921 acaggtgctg tagttgctgt cacccgtcat atcggtgcag ccgttcagat tgccgtcaaa
     4981 ctcaaggttc tcaaagcggg tcttttctcc cattgaatag cccatgatac cgcccatgga
     5041 gaggtatctg taggcgttgg ctgtgatgtc ggcatgagca aagctgtcta caacatttac
     5101 tctgtttgcc atgcccaaaa gtccgccgac atatgcgccg ctgtttccgc ctacgccggg
     5161 aacatatgga gtttctgttt ccacattgat tgtgcctgtg aatgatgagt ctgccatgcc
     5221 gttatgcgga taattggagt ttgcgttcca gcccacaagt ccgccgagcg ccacacggtg
     5281 ctgaccgctg tcgccgcatt cggacttaaa gcttattgta ttctcctcct tgaccgaggt
     5341 tgtatggcag ttctccacaa agccccatac caagcctgca agaataccca cattcgggct
     5401 gaataccttg ctgcctgcaa tatcgatttt gccggaaacg gtaaggttcc tgattcctgc
     5461 cgtgtcgccg gctccgcgga catagccgaa aagacctaca ttggcgaggg tgttgtcggt
     5521 aatcttaagg cctgtaatct tatagccgtt gccgttgaat atgccgccga accatcttga
     5581 gccgtcgccg atgggtatcc attcgatatt ctgaaggtca atgtcgttta taagatttaa
     5641 tgtcttgccg ttgaagttaa ttccgctcgc aaccagcgcg gagagacctg caagctgctc
     5701 agccgtgctg atgtcatatt cggaaagagc ggcattatac cacgaggtat cggcaaagtc
     5761 tgcccatgtg ggaaccttaa tttcattgcc aattaccatg ctgccctcgg ggaaggtgtt
     5821 gtctgcgaaa attgcgttaa ggtcggcaga gccggattct ttaagtgcgc caaggcttac
     5881 gccgcttacc gcattgccgc tgaaagcaaa tgctgccgcg cccgaaatga cgcctgcgaa
     5941 atgtatgccg atgacttcat cgaacgctcc gctgtttact gccgtggtgc cgtcaacagt
     6001 attgcctgtg aataccgccg atgcctctgc tgagctaaga ctaatgcctg tccaaacgcc
     6061 ggatacgcct gctgcggctg ccgctgttac agtgttgtcg tcatatttcc attgaccaga
     6121 accgctcagc gccgagctgt ggactatgcc gttttggaaa gcattaagcg catttccctg
     6181 tacgctgccg cctgcagagt ttgctttagg ctctacttta agggctgtgc cgcctgtgat
     6241 ttcgttttcc tctaccactg cgccaatgcc ctgaatatgt aaagctgtgc ctgcggcttc
     6301 aatatagttt gctctgacca ctactccgcc ccttgcgttc ggcgctgtgc tgtcgctgcc
     6361 gaaatagagg gcggttttat ttgcctttac gattactcgg ttattggcaa actcaatatt
     6421 ctcgcctgtg aatgaaagct ccttggcaga acccgctgtc tcctgctcat ttataaaggt
     6481 taagccttta attacaacat tatcggcgct tacattcaaa agtgtaaggt ctgaagtgtc
     6541 taaaccgtcg gggtatgtta cgaaagcttc atatattctg tctccgtcac catctgtatc
     6601 gtaattgagt ccccaaatgg tgagaggctt attgactgtc agccgctcct tgtatgtgcc
     6661 ggatattacg aaaattacat cgcccgcatc ggccgcgtca atagcggcct gaatgctgcc
     6721 gctgggcaac actaacgcat cgcccacaaa aactgttacg gatgctgttt tgcgtccgtc
     6781 aaccgtgtaa cggtgatgga tgcaattccg ctgcccgcag ctgttaccaa gccgttctgg
     6841 tctactaccg caatgctttc atcgcttgaa ctccagatta catccttatt ggtggctaca
     6901 tcggggtaaa cagtggctat gagctgttcg ctctcaccca cctgcaaaag gaggaacaac
     6961 ttgttcaagc cgactgagcg aaccggtatt ggtttgacta ttcccagtag tccgaaaaca
     7021 aagtttttaa tggagtcgat gaaatttctg attaggtcca caagaccttt cagcctttca
     7081 aggaagccgg ggatataatc ctcctctgct tccagcgccg tgacatcttc tgccgcggcc
     7141 gcgaaggcta cgagattaaa tgacgaaaat gccaagagga atacgagtac caacgctaat
     7201 gttttgctca aatacttcat tttaaacccc ccttgtgatg aaatccgatt ccgtttagtc
     7261 agtccgctta aaggtttagg cgctgaaaca ttagccgttt acgatatcct gttatcacct
     7321 ccgttttagc ctttaacaag caaatatgaa aaagtgccaa tttattacat ttactgcgct
     7381 aaaaagatta aacttaagta cattaacctt aaacataact taaaattgac aatattttct
     7441 aaagcaagta taatatttca taaaaatttt gtaaatgaag aaaaattttt gtttaagatt
     7501 atattacata atgctcacat atatgacaaa atgacgcaat atttgagcct attagattta
     7561 ctgttttggc aaatttaacg caaaaagaag tgagccgaga atactctcgg ctcacttcca
     7621 aagggaaaaa actatgagaa aggttttatg tcggctgatt aagcttgtat aatcctgtta
     7681 ttaacatatt ggcaatagca agctgcgaaa ttcgctcaaa ggcgtgaacg ccggggatga
     7741 ttccgtcgcc gtagaagtag ctttgcaggc ttacatccgc aaggctgttc agcttgcact
     7801 caccccatga tgtaatgctg atgattcttg ctccctttgc tttcgcgtct cttaccaggt
     7861 ccagaatcac ttccgtgttg cccgagtaag agatggcaag ccccccatcg cttttatcgt
     7921 actgttctct gagagagggg tagtaatcct cctgagacct taaaagctct acatgctttc
     7981 ccgccattct gaggtagtgg tgtatatggc gggcgactat ggaggaacct ccgtagccta
     8041 agattttgat aaaggacgcc ttgtttatta ggtctattgc cttaaagagg ctttcctcat
     8101 taacaatctt tttggtttcg cccagaatat aggccatgtt gtccagtaca aaggcaacaa
     8161 gctgatcctt gctctccaga gtgcttatat gggagctggg aggggcggga gtttccgcaa
     8221 ctgtgctttg cattgccgcc tttttcagct ctttgattcc gctaaggcct atgcttctgc
     8281 aaaaacggac tacggtaggc tcactcacct tgctctcctc cgcaaggctt tttatgttgt
     8341 gctcaattat gctgtcgccg agattcagta ccacatcggc gacctttttt tctgcagatg
     8401 tcaggtcatc gtacttgcgc attatgcgca aattcaagct gacgggcaaa tctacatcca
     8461 cattcactgt gtactaacct ttctttctga taatttgtat atttttacga catcgtatat
     8521 agtagcactg agcaaaagcc ttgtcaaatc aaagagagag ctttttattt cgcaagcaga
     8581 tttcttgccc gttttgcgat attttcgctc gtcagaccgt attcattaag aacttcagcc
     8641 ttgctgcccg actgaccgaa acggtcctct atgccaatgc ggtcaaagct tatgccgtct
     8701 ttgcccacga gaaccgccgc gacagcttcg ctgagtcctc cgagcacatt gtgctcctca
     8761 acgacaagga cctttttgca cttatccgcg tattcaagta tggtttcctt atcgaggggc
     8821 tttattgtat gaatattcag aaccgctgcg tttatattat ccttttcgag ctcctccaga
     8881 gcctcgagaa caaggcttac ggggaaaccg gtggatataa tgcagatatc ctcaccgtct
     8941 ttaagaatct gggccttgcc gatggtgatg tcctgatttt catcggttat ctcgggaacg
     9001 ccctctctgg atgtccttat atatacgggg cctttgatgt cgtaagcctt tttgacaagg
     9061 acctttgcct gattgtagtc gcaaggtgag aggattgtca tgttggggat tgccctcatg
     9121 ctgccgaaat cctcaatgca ctgatgggtg ccgccgtcgc cgacgggggt gatgcccgaa
     9181 tggctgccca cgaactttac atttgccttg ctgtaagcgg cggcgttgcg gatgatttca
     9241 cccgcgcgtc ctgccatgaa taccgcaaag gtggaggcat agggaacata gcctgttaag
     9301 gaaagtccca ctgccatgcc cacaaggttc tgctccgcga ttcccacatc cacaaaacgg
     9361 tcggggaatt cctttttaac agccgatgag cctgttgcca tggcaaggtc tgcgtcaaga
     9421 gcgatgacat cgctcttttc cctcattagc tcggcaagtc cgtcgccgta gccctgacgg
     9481 gttgctctta atttatccag tttcatactc ggcctcctaa ctctgctttt gccttctcca
     9541 gaagttcgcc ctgcggaact ttgccgtgcc atgcgaaatt attctccatg taggagacgc
     9601 ccttgccctt tatagttctt gcgataatgc aaaggggctt gccttggggc ttaatgtcaa
     9661 gggctttggt gatttcctcg aaattatggc cgtcgatttc agctacttca aaaccgaagg
     9721 ctcttacttt gtcagcaagg ttaccgaggg acatgacctc atcggtggag ccgtcaagct
     9781 gaaggccgtt gcagtctgct atcatggtta aattgtcaag cttataatgg gcggctgcca
     9841 taagagcctc ccagacaatg ccctcctgca gctcgccgtc gcctgttaag acatagacat
     9901 ggttgtcctt gttctgcttt ttaaagccga gagccatgcc taccgcgacg gagagaccct
     9961 gacccagaga gcctgtgctg cagtcaatgc cggggcattt attcatgtcg gggtgaccct
    10021 gcagcttgtt tccgaaatgc ctgagccttt taagctcttc cttctctata tatcctttat
    10081 cggcaagaat tgcatagaga gggggatttg cgtgaccttt gctgaggata aagcggtccc
    10141 tgcggtcatc tcttgggtca tcgaaaagtc gcatcttgcc gtaatacaaa acggttaata
    10201 tatcggttgc ggaaaaggag ccgccgggat gtccggagcc tgcggattca agcatgtcaa
    10261 gaacggtgcg gcgaataaac gctgcctttt ccttatataa atcgtatgac atttaatcct
    10321 cctgaattta agctttattg acgctgccga aaagctgcat tttctcaatg gcgacctgct
    10381 tgcttgcctc aagaccggga gccagcagag ctctgggggt aaagcctttg cctacaaggt
    10441 ccctgccctc ctcaatgtac tctctgactg cctgggtaaa ggctatctgg cactcggtgt
    10501 ttacattgat tttgcagacg cccatggaga ttgccttctt tatcatgtcg gttgggattt
    10561 ccgagccgcc atgaagaaca aggggaattc cgtttgtggc tttttggatt tcctcaagaa
    10621 tgtcaaagcg aaggcccggc cagtctttgg gatactttcc atgtatattt cctatgcctg
    10681 cagcgagcat atcaatgccc agctcggcca tttttgcgca ctcattcggg tcggcgtatt
    10741 cgcccatttt tgcgactccg tcctcttctc cgccgattgc gccgacctca cattcaaggg
    10801 agatgccctt ttctctgcaa agtcctaaga tatagcgggt tttttcaata ttctcctcaa
    10861 ttggaagggc tgagccgtcg aacataactg agctgaatcc cgactcaatg gcgtcgagaa
    10921 cttcctcgta tgtgccgtgg tcaacatgca gggcaacggg aacgctgatt ttaaggtagt
    10981 caataaacga agttacggtg gcgactattg ttttatatcc gcccatgtac ttagccgtgc
    11041 cgcctgaaac ccccagtata accggggatt tcatttcttc ggcggctaag aggtaagcac
    11101 ggatggattc catattgttg agattaaaat gccctacagc atacttgccg tttttggcat
    11161 cttgtatcat ctcttttgca gatacatacg gcataaatta attacatctc ctaattacat
    11221 aacgatatct ttgttcttgg atactctcat gaagagagcc agagaaatta cggtaaagag
    11281 aagcagaatt gtggataccg cacaagctgc gccgtctgta ccgcggctta ccaatacata
    11341 tattgaaagg gtaagggttt gggttcttgc gctgtagagc atgatggatg cagacagctc
    11401 ggtgattatc gatacccagc tgagaatcgc gcccgaaacg atgccgcttg ccatcatggg
    11461 agtggtaacc ttaaagaagg tcttgagttt cgacgcgcca aggcttatgg atgcttcctc
    11521 cgttgtgatg ggtatctgct ggagcactgc gacgctgctt cggattgtgt aaggaatcct
    11581 tcggatgacc atggcgataa tcataattgc catggttccc gtaagcacca gccagccctt
    11641 gctgaaggtc agcaccagag cgataccgat aacggagccg ggtatgatgt aaggaatcat
    11701 tgacattgtg tcaaggacat tgttcattgt gttcttgcgg cggacaacca tataggcaat
    11761 gagaattgac atcagaacga caattaccat tgtggaaaga ccgataatca atgtattggg
    11821 cagtgctgtc cgcacatatt tccacgcatt cttgtagctg ttaagcgaaa gtcccttggt
    11881 aaagttgccg aaggcgtttg tgtttctgaa tgagcagtaa acaaggtaaa cctgcgggag
    11941 catgcttatt gcaaccacaa tgtaggtgta agcatgaatg aagaacgaag ttacgggctt
    12001 tgctttcttt ctttcgattg aatgcagagc gttcatcttg aagctgaact tctccgacgc
    12061 gaccttctga acaaggaaaa ctattgccgt gattacgata gcgatgacgc tgattgcggc
    12121 ggcaaagtta tagttctgag aagtttcgcc cacatactgc ttgtaaatct caacggggaa
    12181 ggtacgatag ccctcgccga taagcagcgg tgtgccgaag tccgccatgg ctctcatgaa
    12241 taccataaga gcagcggcga gaatagaagg tgtgcaaagg ggtacaacca ccttgaagaa
    12301 acgctttacg ccgctgactc cgaggttctg agaagcctca aggagtgagt tgtccacatt
    12361 tctgagcgcg cccgaaacat acatgaatac caagggatag agccttgtgg tgagaaccag
    12421 aacgactccc ctgaagccgt atatgctggg cagcgcgatg ccgagctccg cgaagaactt
    12481 ggttataagt ccgtagcgtc ccaaaagcag aatccaggca taagcgccga caaagggcgc
    12541 ggacatggag ctgaggatta taagaacctg aacaaccgaa cggccatgga ttctgtagag
    12601 gttgtaaagg taagccagcg gaacgcccac aagaagtgaa aacaatgtgg tggcgattga
    12661 aatcttaaag ctgtttaaaa tggtgttgaa ataatagggt tcgctgaaga actgaataaa
    12721 atactttaaa gtgaatctgc cgttttcata tacggattct ttgaggatat tgaacatcgg
    12781 cactatcagg aacagcagat aaagcagcgt tattgctatg ctgattaaat gccagagctc
    12841 taacggcctt tttcttgact tggtcataat aatcctcctt tagcctatta gcgctctttg
    12901 tccgtcttca gagaaaacat taatcttgtc ggttttcact gaaatctcta cttcagaatt
    12961 cttctcaatg gtgtcctcaa ttgaggattc acgggtaatc tgtacctttt cgccgctttc
    13021 aaggcggacg aagtactggg tgttaagacc caaaaatacg ctgtcttcca ctattccctt
    13081 taatgagccg cagccgtgtt cgcagattac gaattcctcg gggcggattg agagcttgac
    13141 attcaagccg tctacggcaa ggtcgttaag attcttcatc gggaacttag cattttcaag
    13201 cattacgccc ttttcgccgt tctcctcata aatcttgccg tcgagaacat tcgtcttgcc
    13261 tatgaaggtc gcgacaaaca agttggcggg acggtgatag agaaccttcg gtgtgccgat
    13321 ttgctgaatt acgcctttat ccataacggc gatacggtcg gaaatagcca tagcctcttc
    13381 ctggtcatgg gtaacataaa ccgtcgtaat gccgattttg ttctgaatct ctttgataac
    13441 ctcgcgcatc tctaccctga gcttcgcgtc aaggtttgac aagggctcgt ccataagcag
    13501 aacatcgggg tgtaccgcaa gagctcttgc cagcgcgact ctctgctgct gaccgccgga
    13561 aaggcgttcg ggcagacggt tttcatattc ttttattttc attaactcga ggaactcgtc
    13621 cgtcctcttc ttaatttcct ctttgctcat tttacggttc tttaaaccga aggcgacatt
    13681 gcctctgacc gtcatgtgcg ggaaaaccgc ataattctgg aacaccatgc ctatgtttcg
    13741 cttggcgggg tttaaatcat tgatgcgggt ctcgttaaaa taaaaatctc cgccctctat
    13801 tgtattaaag cccgctatca tacgaagcaa ggttgttttt ccgcatccgc tggggccgag
    13861 cagagtgaag aattcaccct tttttatgtc aagtgataaa tcggggatta tcacaacatc
    13921 gccgtagcgc ttaactgcat ttttaatgat gatatttata ctcatcccaa atctcctctt
    13981 gagtggcagg aatccgcagg agcggattcc tgcctataca atgacctcac tacttaattg
    14041 tacttagcct ctttaattgc ctgtaaattg cacttattgt ttttttgctt taattgtact
    14101 taacataaat ctgctggaac ttctcaagga tcttgtccct gttgtccttc agatactgat
    14161 ggtcggactc gacaagctta atcttgctga tgtcggtcag atactggttg gttgtgggaa
    14221 gttttgtgtt ggcctgacgg gctgtggatg atgcccaaag cttctgagcc tcttcggata
    14281 caaggtagtc gataaagagc ttagcaatct ccatgttctt agcacccttg atgattgcgg
    14341 atgcgaacgg gaatgcggat gtgccctctt ccatataaac gatcttaacc ttgtcgcctt
    14401 cgccgccttc aatataggat acgcagggag cctcataggt aaggcctact acatactcgc
    14461 cttcgtaaac tgacttgtag cagttgctgg agctgttgcc tacaacaacg ccgtttgcaa
    14521 ggagcttgtc aacatagtcc caagccttct cgctctcata gccgcccatt gcaacgagca
    14581 ttgtggcgag catattccat gcggaagatg aggaggcggg gtcagcccag atgatcttgc
    14641 ccttgagagc ggggttaaga agtgattcaa agccggtaat gctgtcgggg tcgatgcctg
    14701 ccttcttagc ggcgtcaatg tttacaagca ggttttcaag ctgaatggtt gtccagctga
    14761 gatagcctgt tgtctttctc atcgactcgg gcattgtgga gtcatacttt gaaacatact
    14821 tctcccagat tccaaggtcg gcgtcggaat atgcaaggcc accgaacatt acatcgccct
    14881 gcgggttctc gatctcagac ttaattcttg tcttgagttc gccggcacct gcctgaacgg
    14941 tttcaatctg gacattgggg tacttcttct ggaagccttc ttcgatgatc agcttgtact
    15001 caatgtcgta ggtagtggtg tagataacca gtttaccttt tacagactcg tccggtccgc
    15061 ttgcgggagc cgatgtgcct ttgtctgtag tgtccttatc tgccggcttg cagccgaaaa
    15121 gtgccaatac tgtgcagaat accagcagaa gagcaacaaa tttcttcatt tattattcct
    15181 ccaattttta ttattacagg cgaacacctg caaagggctt ataattattc tctaccgttt
    15241 tccagtcagt tattcctgtg cggcctccta tatgctcgca ctttaaggca gcggttaact
    15301 gggcaattga tatgcagtcc tcaaggtttt tgccctttaa ataagccgca aggaacgcac
    15361 cgtgaaatac atcgcccgct ccggtagtgt ccacaacatt caccttgtag ccctcaaagt
    15421 gataaacatg attgtctatt accgcaaaga ctccgtcggc tcctgctgtt gacattacta
    15481 tttccgggcc ccatgtactg atttcaagca gcgcttcctt aattgtaggt ttgcccgaaa
    15541 ctgcggttgg gtataccgcg ctcatgatta aaatgtctac tctggatgcc agttccttgt
    15601 tcaatcggtt atctttctgc ctggtgctgc cgtcaaggga tatcagaacc ttatactcct
    15661 ttgccaattc cgcggcgttg acggcgtttt cgtaatccgt gccgtcaagg tgcagtatct
    15721 tggcgttttt cagcatattc tttttgtgct cgtcccagac aatcggcgga atgttgccct
    15781 tgtaagggaa cttggtcctt gttccttttt ttgtgtcaat cattacatag gaacgaaggc
    15841 tttgcactcc cggcaaaact tcgacgcccg acacatctac gccttctctt tttaattctt
    15901 ctaagatcag gcttccgacc tcgtcatcgc cgatattgcc gataaacgct gattcgattc
    15961 caagccttga agccgcaacc attgccgtcg cgacggctcc cccgccctgg gtgctgacgg
    16021 atgttatgcg ggttgacccg tcctccggcg gatactcatc aatcaaacaa agataatcgt
    16081 ggcagcagtg cccgacaccg acaagactga ctatgccaac acctcctttt gttcgaatca
    16141 tatcattctt atcagaattt gtctatattt tatagtaaaa ttatcagagg gttattatgg
    16201 aaattttttg tattaatctt cctgtgattt aagtttcaga cgcatatcga gcgtcagagc
    16261 aaaggaagtt tccctctgaa aatccacttt attattccaa atgaaatacg gaatataaca
    16321 aaatcaaata tttaaggcct tcggcgcggc tttatttctt tactgattta gtacttttcc
    16381 acaataaatt tgcgaatgaa atattaaaat ttgtagcata tgaaaagcaa aaaaaatttt
    16441 ttataaaatt tttcgttgtg cgggggaaaa tagtatgata tattcacaaa aaagagccgg
    16501 aggggttaat ttgagcaaaa attacgatat tgcggcatat atctggaccg cttacacagg
    16561 cgatgagccg agagcgagga ttttttggaa ggaaggcatc ggcgaatggc agagcgtcaa
    16621 gaatgccgtt aagaagttcc ccgaacactc atggccgaga aagccgcttt ggggctatgt
    16681 gaatgaagcg gaccccgagg ttatggaaat ggaaatcgac tgcgccgcaa agtacggagt
    16741 gaatgtcttt atatacgact ggtactggta cgacgacaga cctttccttg aaaactgcct
    16801 gaatgacggg tatttgaaag ctaaaaacaa tcatcttgtg aagttttata taatgtgggc
    16861 gaaccacgac gctaacaatc tttggaacat tgacctttcc gacgactttg gcgatgtggt
    16921 catctggaag ggcgcagttg gctttgacag tttcaaagtg attgttaagc gtcttatcga
    16981 gaaatacttt acccaccctt cctattacaa aattgacgga aagccagtgt ttatgattta
    17041 tgactttaag aattttgtca gcggacttgg cggcagcgag ggagcaaaga ggggcatcga
    17101 atacttcaga aacgaagcaa agaaggcggg attccctgat ttgcaccttc agcttgcggc
    17161 gtggggaaag cacaatctaa accacagcgg agttgactca aacggcggcg ccgacagctg
    17221 cgattataag gacttgggct ttgacagcat tacaaattat cagttcgtgc actttacagt
    17281 tccgaacagg ccctatcccc aaattcttga agatgtaaaa cgggagtacg agaggattga
    17341 caggacatac gacatcccct acttccccca tgtttcaatc ggctgggaca acaaccccag
    17401 atacaagcgg ctgaggaagg atataataac cgaaaacacg ccagagaact ttgaaaaggc
    17461 tttgcggatg gcaaaggatt acgctgatac ccacgacctg cccgcaaagc ttataaccat
    17521 aaacagctgg aacgaatgga cggaaacaag ttatctccag cctgacgacc tttacggtta
    17581 cggctatctt gaggcgatta aaaaagtgtt taaagacgaa taaaaaagga gcccgtttcg
    17641 ggctcctttc ttttaggttt attcaaattt gatgccgccg ctgtttcttg ccattatgcc
    17701 gtactttaca agtatttctt tgtccgccgc ggcaaagtac ccgctgcgct caaactcggc
    17761 agcattgagt tttcttatct gctccaaagt atcattaagc tgcagcatgg cggcgaactc
    17821 atgcctgcaa ttcagacaag agaagcagga gcatgtgagc ttgctgactt cgccgttttg
    17881 gtattcgaac tccacctcat agaccttgct gccttggact accgcatagc cctttgtctt
    17941 gtcaaggcag atatatctta ccttgttttc aaagtaataa tcaaagcctc tttttgtttc
    18001 atcgccgccc acattcattc cgctgagctc atcaagacga aaggatgtat cgtcatagcc
    18061 gctgacgatt tcggcgtcct ccgctattgg gggcataaac cacaatgacg ccttttcaat
    18121 cggcagagcg ttagggtcaa aggttaagat gtgagagcct gcgatgtaga actgaccctt
    18181 gatatcagta tcaattacgg ctattacccg cttgtaatcc gcaagatcaa ttttaaagtt
    18241 gcagttaatc tccacaaccc tgccctgatt gccctccagc ttgccctcaa catagaccct
    18301 gtcgccgagt ttcaggtcaa aacggtcgtt gtaatatgta taggcgcggt ctaaactttc
    18361 aaaataaacc ctgaccaaag acttgcgggg ttttgtttct ttgggaggga catatgcctc
    18421 cactttcggc tctatattag gaatatcagc gttaaaacct aatttaaatg acataaaaaa
    18481 cccttcttta cctaaacttt ggagaattct gtgagttaag ccgatttacg actctgtcaa
    18541 gcagagcttt gaaaaacgct ctaaagcttt tgttaccgcc aaagcgtttt accaacgctg
    18601 ggctgaggga atagtatgcg cggatgaaaa gtctgccgag aaaagtcttt gagagcgtgt
    18661 tatctctgaa gcgtcgcagc gtcatgacct gtgggctttc gtatgagccg taaacacagg
    18721 tggctatgta gcagccctcc ttggtgtttt tatgctgata gccgccgaat aagccgtggt
    18781 cgctggtgct caccattctg cctttagcgt ctttgtggac atagcctttg aaatttttcg
    18841 actccgactt gcctattatg cggcctcgct tgtcatagtg ggtatatccg ccgaaaattt
    18901 tgggcgacga atagcctatt tttcttcctt gtcggtcgta gtttatatat ccgcctataa
    18961 tgctcggctc gctcttgcct atttttctgc cgttttcatc ataatgatgt atggcgccaa
    19021 ataaatcccg cttgctcctg ccctttcttg ccacaaacac ccctccgtgc ctgtaaagtt
    19081 actcccatga ttataatatg attttaagtg taccattaaa gtcacatagc gcgctataaa
    19141 acggggattc gctcaatttg gctttaattt tcctttttaa caataagttt cgttgacact
    19201 ttattgcaaa tttcgtatta ttatgtcaag ggagtgagcg gaatgatttg tcccaaatgc
    19261 gggagtaaga cattggttga aaacagcgta ttctgcaatc agtgcggcta tagttttatg
    19321 gatggaaaag ccgcgacaaa taaatggtat gagaacaaag cagttgtgct tttgctcatt
    19381 tttcttttct accccgcagg cttttatctg ctgtggaaaa tgaatcctaa gaaaagcgtc
    19441 agagccgtta ttgctttagc agtaatcttc acaacacttt tttggttaag tattataggt
    19501 tttattggtt ccgagcttga cgcgagcacg acttttgata attccattaa tacatccgtc
    19561 gccgaagaag aaactccgga cgccgaaagc gcaaagaaag agtatgttgc cgcctgcaag
    19621 acttattcat acaaggagat tttaagaaat cccgcaaact atgagggaga gaaggcttat
    19681 ttccagggca aagtaattca ggctcaggaa tcgggcggaa aaacagtgct gagagtcaat
    19741 gttacaaagg gcgagaacaa tgcatggacc gataccattt atgtggagta cacaaagaaa
    19801 gacgaaaacg aaagccgcat attaaaagat gacataataa aaatctacgg tgagcttgac
    19861 ggaataaaaa atataaccgc atcagggcag aaaaccaatg tcccaaaaat tgctgccgaa
    19921 tacatagata tagtagaata aaaaaagcac ctgcaatgca ggtgcttttt tatgcgctgt
    19981 tgtccgctct tttcttattt gcccagctct tttatttttt cctcgattgc ttttacgcgg
    20041 tcggggcggt tgaagttgat attgtcttct ttcagcattt cgaggctgga aatatatatt
    20101 tctttaccat tgaaattaac tttcccaagg gtgctcatgt cgtagggctc cagctcttta
    20161 ttgtggaaag cgatgctgat aaccgcgtcc ataaagattt ctccgagtcc gctcataacc
    20221 cagttttcgc agcgttcgat gggtcttatg gcaaacttgc ggaaatggtc acgcacttta
    20281 tcaaaatcgg agtaatttgc tactatgatg tttacatctt tgggctcaac atcaattccc
    20341 caaagagcca tcgccgcgct gccgtggaca taccagtcga cgcccagctt ctgaatttcg
    20401 gagcaaaacg attccagagc tttctgccat ggggtttcta ttagtttaag ctgctgcttt
    20461 acggcggttt cgccgtactt tgtgaagttc ttggcgcagt actcaaagtc ccttacattg
    20521 tcgtaggatt ttataaagtt attgccgacc tgccaccatt gattctcagt gaaataatcc
    20581 cagtgttttt cttccacatc ttcggcgata aaagcgtagt ggttccagtc atacatctgc
    20641 tttacataga ttttcatatt aagccctcca atctgttgct tgacgccggc taaagggata
    20701 agccggcttt tttcggcggc gggagaaggt ttttgcagct ttcggtagcc ccagggggtt
    20761 attccgtaaa cctttatgaa ggctcttgta aacgcctctt gggatgagta gccgtggcta
    20821 acggcgatgt cgattattcg atgattggta ttgaggagcg cagcagcgga atgctgaatc
    20881 tttgaaatga ggatatattc ctttagagaa atgccctcaa tctcgtgaaa ctttttagac
    20941 agatagtggg gagaatagcc gcagtgttct gaaatctgag aaagtgttat gccctgctca
    21001 atgttttcgt caatcagagc cttaattatt tctgtcaggt ctacccaaga aagcacccta
    21061 aaacctccct tccgcatcta tgataaaccg aaggctatct tcaaatcttg atatttgttg
    21121 cagtcttttt tcaattgtat aggaaaaaag aaaagaagtc gattgaaatc gacttctttt
    21181 tggcccgctc ggagggattc gaacccccgt tctccagaat cggaatctgt tgcgttatcc
    21241 agctacgcca cgagcgggta tttgccgata atatagccag ccttttggct gactatatta
    21301 tacagcttaa atgtcatgga tacaaagtta tattttgcta tacattgaac ctaaagagga
    21361 ccacatctcc gtcctgcatt acatagtcct tgccctcaag gcggacaagt cccttttcct
    21421 tagcggcagc cattgagccg cactcaatta gctcttcgta ggagatgacc tcggctctta
    21481 taaagcctct ttcaaaatcc gaatggattt tgcccgctgc ctgcggagcc ttggtgccct
    21541 tcttaatggt ccacgccctg acctcctgtt ctcccgcggt aaggaaggag ataagaccta
    21601 aaagcgcgta gctcttttgg ataagaaggt caagtccgcc gctttttatg cccgcgtcgt
    21661 taaggaagat ttcgcgctct tccttgtcaa ggtcggcaag ctgagcctcg aattctgcgc
    21721 aaatcggcag aacctgtgag ccctctgcct cggctatttt ctcaacagcc ttgtagtatt
    21781 tgttgttttc caggttgtcg gcaaagtcct gctcgctcat gttgcaggcg tagataacgg
    21841 gctttgccgt aagaaggggc acggtgctta aaatctcctt ttcctcgtcg gtcaagggga
    21901 ttgcccttgc gcagacgccg ttgtcaagct cggctctgac ctttttaaat acctcaaggg
    21961 atttcgccac gctcttgtcg ccctttaaag ccttggtatc cctgtcaatg cggcggtcaa
    22021 gaatctccgc gtcggagaga atcagttcaa ggtttatggt ttcaatgtcc ctttgcgggt
    22081 ctacactgcc gtcaacatga ataatgttgt catcctcaaa gcatctgaca acatggatga
    22141 cggcgtcaac ctccctgata tgggaaagga acttgttccc aagaccctcg cccttggagg
    22201 cgccttttac aagacccgct atatccacaa actcgattgt ggcgggggtt actttatggg
    22261 gattatacat ttcggcaagg cgggtgagcc tttcgtcggg aacaggcact atgcccacat
    22321 tgggctctat tgtgcagaag gcatagtttg ccgactgggc gcccgcgttt gtaagcgcgt
    22381 tgaaaagagt gcttttgccc acattgggca gtcctacaat tccgagtttc atttgattcg
    22441 tcctgctttt taaaatttac tttgctattt tattatcttt agaacaatcc gtcaacatta
    22501 agcccgaatt tatagtattt ttgaatcggc tcggagacag cccaggtgca ctcaagtccc
    22561 ttggggaagt gaaccagcat tccggcctct atatgggttg tcttgccctc ataatgaatg
    22621 tctgccgcgc ctttgaggat atagcaggtt tcggaggagt cgggatagct ccagtcaaag
    22681 gtttcggctc cgcagcccca tgttccccat gtggcgaagt taagtttctt gtcagcctca
    22741 tccttgtcaa tcttttttgt cagaacttcc ataatgcctc tcctttcata tcgggaattt
    22801 ccgcccctta cggggcggat tttttagtat gttaccttaa ttgttttaat ctcaaagggt
    22861 ttgaaggtca tttctatgcc gtttgcccaa acctcaagct ccttctgaac ctcttcaagc
    22921 atattggttt catatgcctt gtcgatgccc tcgcagaagt atgctctggt gttggtgaag
    22981 gtgccttcag cctcgtagaa gcgtacgatg aacgctcttt cgcaatcctc gcagggcttt
    23041 acagcctcaa caatgatgtt gggggaaaca actgtcataa gctgttttgc gctgtacttg
    23101 ccatttgcct gaacgggcgg gacattgagc atgtatgcgg gatggattac gctctcgctg
    23161 ctgaagccgc agttatgggg cagcagagca taggtgcatc tgtggatgcc gtccttgtct
    23221 cccttttcgt cgggtcttgt gccgcccttg tggagagaca gatacattcc gctgccgttt
    23281 aaggtaatgc catacttgca gtcgttgagg acgctgacgc cgtacttggt ttcggacaag
    23341 tctgtgtact tgtggtttac aacctcgaac ttagcctgct caaggctgtt gttgcgggtt
    23401 gtgggtctct ttacatgacc gaactggatt tcgttgcggg caaagtcctc ggtgatggtt
    23461 gtttcaaagg cagcctttaa gaatctgtgg tcgtcgttcc agtccatggc ggtgtcgaac
    23521 ctaatttcag cggagttggc gaagaaaatc atatcctgct tgattgttga cttctcgctg
    23581 attctgtatt cactgcggac gatgtacgcg acctcgccgt cggaaacgac ttcgcggctg
    23641 ataagctcca tagagggctt gaacttgctc tcaatatcgg cgtccacatc ccagttgtcc
    23701 catgcggagg ggacatcctc tgccataagg aaggtgttaa gcggcaggcc gcctacaatc
    23761 tccctgtggg ctttcgggtc gtagagggat tcgattgcgc cgttttcgga gaaggttaca
    23821 tcggcaaagg gggtctccag cttgttttcg aaagcctcaa acgcgcttct gccgccgggc
    23881 tcgcccttta cccatttaag tgttacgctg ccgaaagcgg ggatggtaag acccgcaaga
    23941 atcagctttt cgttgccgtc aaggtcggtg tagacctgct gggcgtactc tccctcgata
    24001 atgcagccgg gcttgcggtc gatgaatacc gcgtcacttc tgtcaaatga caatgtattg
    24061 ataagggtta cataatcttc gccctcggag tcgaccgtct ttgcgatgag ctcctttgcg
    24121 gtctttatga cctggcccat tttctctcgg ctttcctcat gggctctggt aatgcatgtg
    24181 ccggggagga tgtcgtggaa ctgatttata agcagctcct catagagggg attaatctcg
    24241 tcgttttttg cttcttcgcc cttggtgact gcattcgcga ctgtcagaat ctcaaggtct
    24301 ctgagagcaa gctccgcttt tctgttattc ctctttatgg tgtgctggtt tgtgagagtt
    24361 cctctgtgca gctcgagata cagctcgccc ttgtagacat tgggattctt aacgctttct
    24421 tcaagctcct tcataaagtc gccgactctg acatgctgag ttttcggaac gcccaaggta
    24481 tccttgattc ttcttgcctg ctcaaccatt tcaaactggg ggccgccgcc tccgtcgcca
    24541 aagccgtagg caaggagtct tttgttggta acggacttct gctttactcc cttgtttact
    24601 ctgtcaaaca catcctccac attggggaag tggtgggttg tgttgaagtg ggagaatacc
    24661 tttgtgccgt caatgccctg ccagtagaag gtgtcgtagg ggaagacatt tgtgtcgttc
    24721 cagcttattt tggtggtgag gaagtagtcc acagagcagc ccttcataat ctgggggatt
    24781 gctgccgaat agccgaaggt gtcgggcagc cagaagcagt ttgaggtgta gccgaagtgc
    24841 tttctgttaa agcgctgacc ccaaaggaac tgtctgacta tggactcacc cgaggtgatg
    24901 ttgcagtcgc actcgaccca tacaccgccg ttgggctcgt atctgccctc tttaaccttc
    24961 tcctttatcc tctcaaagag ctcgggatag tgctttaata tcatattgct gtggcaagcg
    25021 gagctttgaa tgaaggtaaa ctccggatac tgctccataa gcgagagggc gttggagtag
    25081 gtccttgcgc acttcttaat ggtttcgccg atatgccaca gccatgcggt gtccatgtgg
    25141 gagtgaccga taatgccagc ctcgggagca gactcggggt tcttgaccga aagaacctgc
    25201 ttcatcaagg gagcgacttt tctcagggac tccataaagg tatcccagtc cacattatct
    25261 attgaataat agacattgtt gtggacttca tacaggcagt ttatgatttc gcccttgcgg
    25321 aaactgtcat cgggcagagc ctccgccatt tgattcagag cctgaaggtc gaagtagaag
    25381 tcctgaatct cgtagttttt gacgcagatg tccgctccct cgtacttaaa gttatagttc
    25441 tttatgggag cgacctcaaa gggctggcag cctatgcagt ggtgaccgcc gtagtactca
    25501 atggcgatgt ttattttctc ccctgccttg gggttcatct ttatcatgtc gcagtagtgg
    25561 ttgccgtggc ctgtgtagac tatttttgtg gcaaaggtgc ctgataccct gccgtcaacc
    25621 cacagcatgg cctcgtagcc ctccattttg ggctttataa ataagggttt gccttcaagc
    25681 tccgcgggga cggtgtattc gcccttatac cagcagtacc tgttctcgcc ctgccatgta
    25741 aagccttttt cgataggctc aaaaaggctg tcgtcgggga tttcgtgata ctgcttgtcg
    25801 gttacaaagg ctttgaaatc cacctcgccc acctttgtga agatgtaagg ctcaaggcac
    25861 tcctc
//
LOCUS       DAONVQ010000008        25442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_1309_length_25442_cov_4.609249, whole genome shotgun sequence.
ACCESSION   DAONVQ010000008 DAONVQ010000000
VERSION     DAONVQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 25442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25442
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1309_length_25442_cov_4.609249"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /gene="atpD"
                     /locus_tag="PLD98_01230"
     CDS             complement(<1..1116)
                     /gene="atpD"
                     /locus_tag="PLD98_01230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQA04908.1"
                     /translation="MKKNTGRITQIIGAVVDIRFEDGLPSLYNAVEVPHNAVEVPHED
                     RTIVLEVMQHLGDRTVRCISMDSTDGLRRGMEAYDTGSPIKVPVGEEVLGRMVNVLGK
                     PIDNLPEIEAKDYWQIHREPPKLAAQIAQPEMLETGIKAIDLLCPFPKGGKIGLFGGA
                     GVGKTVLIMELINSIAKEHGGYSVFVGVGERTREGHDLYFDMKDSGVLSNTALVFGQM
                     NEPPGVRMRVALTGLTMAEYFRDEKQQDVLLFIDNIFRFVQAGSEVSALLGRTPSAVG
                     YQPTLANEIGALQERIASTKSGSITSVQAIYVPADDFTDPSTATTFAHLDAVTVLSRS
                     IVEQGIYPAVAPLDSSSRILEPHIVGTEHYKVAQSVRS"
     gene            complement(1129..1974)
                     /gene="atpG"
                     /locus_tag="PLD98_01235"
     CDS             complement(1129..1974)
                     /gene="atpG"
                     /locus_tag="PLD98_01235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HQA04909.1"
                     /translation="MASLKGIKQRINNVKSVEQLISAMDIIASTKLHKARAQLEGARP
                     IYQGLKRISEEIGYHEEASKHPFYKSSEVKKSLYLVRTSDRGFAGAYNHNITQKVLEH
                     IEAGGKNAEIIAIGQKGYEFFSRRDFNIACKITDVSDARMYHETEDISKFVLNLFLSG
                     KVQEVFVCYTHFENVLKNIPTVEKLLPLPYSPREYYEELRKYEPDLMTYIEHTIPLYI
                     HMSIFRAFSESHTSEQASRMVSMDSASKNAEEMIDKLTYQYNRKRQAAITQELTEIVG
                     SKSLL"
     gene            complement(1986..4034)
                     /gene="atpA"
                     /locus_tag="PLD98_01240"
     CDS             complement(1986..4034)
                     /gene="atpA"
                     /locus_tag="PLD98_01240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQA04910.1"
                     /translation="MAKLTNRYAEALLELSREKGSFERDLKQAALLKESISDPQTKAF
                     LTYPNVPDREKLSVLQTAASDDAPEHLKGFLRLMVEKSRESLILPILEEYIELLSRST
                     GRLKAKLVSAKELSEKQRELIRNLLSSKTNRQVELECSVDPDVLGGFYILADGRVFDC
                     TVRTELIRMKEYLKKGNIGMTANPAEISSVIKKQIESYSSVADFTEAGTVIQVGDGIA
                     RVYGLGNVMSGELLEFSNGTYGMALNLEEDSIGVIIMGSDAGIKEGDPVKLTGRMAEV
                     PVGDALLGRVVNALGEPIDGRGAIAAETYRKIESPAPSVIMRKAVNQPLQTGIKAIDA
                     LVPIGKGQRELIIGDRETGKTSIALDAIINQKGKDVFCVYVAIGQKASTMARIIKALT
                     ETGATEYTTVVIATASDSASLQYLAPYAGCAIAEHWMYKGKDALIVFDDLSKHAVAYR
                     AISLLLRRPPGREAFPGDVFYLHSRLLERAAALSEEHGGGSLTALPIVETQEGDISAY
                     IPTNIISITDGQIYLEAELFNHGIRPAINPGFSVSRVGGAAQIPIMKKLAGPLRIEFA
                     QYKELVAFTQFGSDLSKDTLMRLNHGEKITEVLKQPNYKPMPVENQVLILYALNNRHL
                     DDIDTWRIQHFQNELIKSVSSQEPAILEEIRNTGEISDQNAKKLDSIISEVKSKHYFS
                     "
     gene            complement(4022..4573)
                     /gene="atpF"
                     /locus_tag="PLD98_01245"
     CDS             complement(4022..4573)
                     /gene="atpF"
                     /locus_tag="PLD98_01245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HQA04911.1"
                     /translation="MNFTILLAQAAVEDRLFGLDSQTLIGALIQFVNVLILTGALSFI
                     LYKPVKEYMEKRSLKIKGQMEDAEKAKQKAEALVAEFEARLKDIDDEAEKILEDARLK
                     AAEEGRAIVENAKHEAEAILKRAQEKIEFDKIKLREESRLYIIELAVNLAQKYIESSI
                     DDNAKEQLFEEAISKLELSKWQS"
     gene            complement(4598..4870)
                     /gene="atpE"
                     /locus_tag="PLD98_01250"
     CDS             complement(4598..4870)
                     /gene="atpE"
                     /locus_tag="PLD98_01250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HQA04912.1"
                     /translation="METLGLIISVAHIAAAICLLNGLLTTFGQAKIASQAIESMARQP
                     EAADNIRTSMFIGLAMAETSGIYGLLIAIIMLFANPLINTFVNLLT"
     gene            complement(4904..5581)
                     /locus_tag="PLD98_01255"
     CDS             complement(4904..5581)
                     /locus_tag="PLD98_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HQA04913.1"
                     /translation="MGIGVKTFWTFNIFGVEVWITETIVNTWIIMLGLIALAIFARIK
                     LRSFKEIPEGIQNFIEAAVDIFDGFAKSNLGEKLSYISPLFFSVFAFILSSALFSVFG
                     LRAPTADFTTTFALAMLSFLMMLIMGFKHKKGKYLKSFFEPYPIFFPLNLIGELSKPI
                     SLSFRLFGNMLSGTIILTLYYALTPIFVQIGIPALLHAVFDVIFGALQTYIFVVVSLM
                     FVKNAAD"
     gene            complement(5594..5968)
                     /locus_tag="PLD98_01260"
     CDS             complement(5594..5968)
                     /locus_tag="PLD98_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HQA04914.1"
                     /translation="MKISALAKRMLISILIINSAAVLIAAIYHRSLSFLPFLYGALIG
                     SAAAVGKVFLLERAVSKSLSMDKKSAGGYVALQQILRLFITGIVLYVGAVSPGISIWG
                     TAVGVLSFQIATYTVKFGKRDS"
     gene            complement(5978..6214)
                     /locus_tag="PLD98_01265"
     CDS             complement(5978..6214)
                     /locus_tag="PLD98_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQA04915.1"
                     /translation="MDKKDDKDNRSKGTSFFKALADFSNIGFSIAASVFVGVFLGKTL
                     DNLLGTAPFLLLVFSLLGVGAAIKFVFDFSKLEK"
     gene            6753..7112
                     /locus_tag="PLD98_01270"
     CDS             6753..7112
                     /locus_tag="PLD98_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04916.1"
                     /translation="MRIRKQISILLALVIALSMLGVFGVACSKPSVEDINKVTTANVF
                     EAIKDAYKDVTGKEMPKEVADKLNDDLYSKIAQGTGYMEYAPYASEFIYEKTGVIIPP
                     ETLAIAIKAAVLVAGNL"
     gene            7262..8038
                     /locus_tag="PLD98_01275"
     CDS             7262..8038
                     /locus_tag="PLD98_01275"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQA04917.1"
                     /translation="MYPYMEIFGRLIPSYLVMALIGLVFTLVLALIRRKKGNLNFQAE
                     DVILMILIAAIGAMLGAKAFQLVGVILRDGNEPGFWTWEHWKSLIPTAGVFYGGFIGG
                     FAAMLIYIRKNKFDFWKVADLMVPSILLFCTFGRIGCFLAGCCYGKVSHTCGIVPLYG
                     AEPLIPVQLYESAFTLVLLIGLLITRPERKREGILLPIYILSYAAARFALEFFRGDMQ
                     RGVFILSTSQWISLLMVPLGIYLLLRNKKHNEKPPAESNE"
     gene            complement(8121..8861)
                     /locus_tag="PLD98_01280"
     CDS             complement(8121..8861)
                     /locus_tag="PLD98_01280"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HQA04918.1"
                     /translation="MSFVDKISELKKYIEFSDNIVFFGGAGVSTESNIPDFRSATGLY
                     SEKGGKRGFAPEQMLSHSFFARYPEEFFEFYKSKMIYKDARPNSAHIALARLEDMGKL
                     KAVITQNIDGLHQLAGSKEVLELHGSVHRNYCLDCGKFFSLDYILSSEGVPKCDACGG
                     IVKPDVVLYEESLDMDILGRAVNYIEKADMLIVAGTSLTVYPAAGLIRYYNGNRLVLI
                     NKSPTPYDNLAKLVINDSIGKTLSACLE"
     gene            8988..9359
                     /locus_tag="PLD98_01285"
     CDS             8988..9359
                     /locus_tag="PLD98_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQA04919.1"
                     /translation="MFFVAFFGMNDKEEHIGAFSGEGCPSCGQLSQFAVSKTYRNLEV
                     FFLPVYKWNVRYIARALCCGAVFELDPILGKELEKNPYIKIRPENLRRIERQSPFKYC
                     PNCRVDVPNEFAYCPYCGGKL"
     gene            9540..10403
                     /locus_tag="PLD98_01290"
     CDS             9540..10403
                     /locus_tag="PLD98_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04920.1"
                     /translation="MLLDHVKKYFAFIWSLLVLFAGLFTRTVAPVDAPNLEVVSNYHY
                     IMDEALVMGQGLANDGTYLYSSGAISALYLGALSKIDMNTGEILLRKINAIPEEFVKK
                     GYDHIGDISWHNGLIYAPVEDLQEEQPLVLLYDAETLEYTGTYFELDNTYLPDGIPWC
                     TVDADNGYLYASPFKNVTCIVAFNLSDMSFSHIIPLSEEITRVQAADYFDGKLYVNLD
                     TQGTGYKEIKVIDVNTGEVSLLAQRDMQGRDVETEGLTITRDENGKLLFHITDYDKTV
                     SVYLRTYRLTD"
     gene            10459..10716
                     /locus_tag="PLD98_01295"
     CDS             10459..10716
                     /locus_tag="PLD98_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04921.1"
                     /translation="MKIRRYIKQYDEENVMEMLKEEGDEWRCYHADEVSAKYKAALQN
                     SITYVAYENDEICGYSRSLDDCGFYIYVCDLLVRKNTGAWK"
     gene            10707..10853
                     /locus_tag="PLD98_01300"
     CDS             10707..10853
                     /locus_tag="PLD98_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04922.1"
                     /translation="MEIGRKLMECIYKDFPDRIVYVMSDADGYYVKLGYRREGSVFEV
                     TKEE"
     gene            10965..11726
                     /locus_tag="PLD98_01305"
                     /pseudo
     CDS             10965..11726
                     /locus_tag="PLD98_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099404.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            12200..12646
                     /locus_tag="PLD98_01310"
     CDS             12200..12646
                     /locus_tag="PLD98_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQA04923.1"
                     /translation="MKRGFDYSSLIGSGNPLKSYPQLYEAAVNEFSMKKYEEASLNDI
                     LKNSGMSKGSFYYHFGDKFGLYLAMMDIITNKKLSYFYPAVQERAGESGFFDTLKDIM
                     KGTMDFMLADERLYHLLNRVLEEDESLRSRMAEFFLMTTKDSSANI"
     gene            12840..13718
                     /locus_tag="PLD98_01315"
     CDS             12840..13718
                     /locus_tag="PLD98_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA04924.1"
                     /translation="MSVAFSVQDLRFKYPSAKEDTLKGISFEVKEGEIFGLLGPSGAG
                     KSTTQKILIKLLEDYKGSVKYFGKDLKDLGKSYYEEIGVGFEVPVHFSKLTALENLKF
                     YSGFYKSKADIRELMERVGLWEYRDKKVGEFSKGMKVRLNFVRALLNKPRVLFLDEVT
                     NGLDPKNARIIKDMIAEYRKDGGTVFLSTHLMNDVEQLCDRIAFCVNGSLVEVSTPRE
                     LKLKYGKREVKVEYNENGNLSTAVFSLDGIGENEEFVNLLKSKQIETIHSGETSMEEI
                     FIIVTGVELNERTEAV"
     gene            13696..14412
                     /locus_tag="PLD98_01320"
     CDS             13696..14412
                     /locus_tag="PLD98_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA04925.1"
                     /translation="MSVLKLFSGEIIRLVKYKILPVGVATTAIWVAILYFLEPREELY
                     LAPMFIFIDVGAMSILLIGASHFLEKQEGTIKSLMVMPVTKREILISKTAASMVLALE
                     SAVITAAALYFIHKITLNYALLFLFVIIAGAAHAAIGFVLSLIGRDFTSMLGIMAAYM
                     FIFTIPSIFYSVGLIDEKYKYLMMVSPSHASNELIRAAVSGEYETGLIIAGCVYLILL
                     AAVLFRFAVYPMFKDKAVRG"
     gene            14417..15208
                     /locus_tag="PLD98_01325"
     CDS             14417..15208
                     /locus_tag="PLD98_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04926.1"
                     /translation="MKKYLWLLKYEAKTIIREPVNVYMALSPLLVLFLSVFVMPMAFN
                     SIDPANHTVLKTTMLFMIILLLAVGSSLLAAMATFLLLEHKDENTLKTIAVTPTGASG
                     YLRFKLTYVYVMSFLGIIMVLLGTKLIAGDKYSINGVSMFDSVNVYHIIAFAAVDSIF
                     APFLALLQSAFAKNKVEGFAYIKGSGIVVLIPALMLLKTFQGSLQYALGVFPNFWAIK
                     AMMLRFMPADSGANLSFPVYLIIGLAYNILLLIVSYRLFLKKAQY"
     gene            complement(15239..15868)
                     /gene="hisIE"
                     /locus_tag="PLD98_01330"
     CDS             complement(15239..15868)
                     /gene="hisIE"
                     /locus_tag="PLD98_01330"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HQA04927.1"
                     /translation="MNLKFDEKGLIPAIVQDARTGKVLMMAYMNEESLEITKKEGRAC
                     FWSRSRRELWRKGETSGNVQKVVSIKTDCDGDCLLIDVEPAGPACHTGEESCFFNEIH
                     DGADVFTYEGLYELIKGRKENPKEGSYTTYLFDKGLEKILKKVGEETSEVIIGAMKKS
                     KEETIYEIADLAYHVMVLMAEMGISLNDIRAELASRHVIDKKTKQETMK"
     gene            complement(15858..16631)
                     /gene="hisF"
                     /locus_tag="PLD98_01335"
     CDS             complement(15858..16631)
                     /gene="hisF"
                     /locus_tag="PLD98_01335"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQA04928.1"
                     /translation="MVSKRIIPCLDVRNGRVVKGVNFEGIRDMADPVEMARYYNESGA
                     DELVFYDITASAEGRKLFTDVLRAVASEIFIPLTVGGGINSLEDFDTVLKCGADKVSV
                     NSGAIKNPDLISEAAKRYGSQCVVLSMDVKRVGGSYRVFAKGGREDTGLDALEWAKRG
                     EALGAGEIVLNSIDTDGVRGGFDMPMLRAIGEIVSIPLIASGGAGKMEDFAELFKYDR
                     IDAGLAAGIFHTKEVSIKELKLYLKEHGVNVRTEGLYES"
     gene            complement(16635..17348)
                     /gene="hisA"
                     /locus_tag="PLD98_01340"
     CDS             complement(16635..17348)
                     /gene="hisA"
                     /locus_tag="PLD98_01340"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HQA04929.1"
                     /translation="MQILPAIDLRDGKVVRLYQGDYDKMTVFGDRPIDTALEFKRAGA
                     ENLHLVDLEGAKDGEPRNFNIIADILKYSGLKVEVGGGIRTEERILKYLEAGARRVIL
                     GTAALKNFEFTAEMVKKYGRHIAVGVDAREGFVAVSGWLETSSVDSFEFVRRLKEIGV
                     QTVIYTDIACDGASKGTNLAAYEKLCEIGINIIASGGISTMEELEKLEKIGAWGAILG
                     KALYSGALDLKTVIHKFGG"
     gene            complement(17359..17964)
                     /gene="hisH"
                     /locus_tag="PLD98_01345"
     CDS             complement(17359..17964)
                     /gene="hisH"
                     /locus_tag="PLD98_01345"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQA04930.1"
                     /translation="MIAVVDYGVGNLFSLKSSLKFLGLDCVVTSKKEDLIKADKIILP
                     GVGAFGDAMMKLRETGLVETVKEQAKIKPLLGICLGMQLLFDKSLEFGEHEGLGLIRG
                     IVAPLSDDIDPSLKIPHMGWNRLRIIKDDPIFKYFKNGESVYFVHSFHAKDCAESVLA
                     VSEYGTTVTGLVRQGLVYGAQFHPEKSGNAGLRLLKAFAEI"
     gene            complement(17961..18536)
                     /gene="hisB"
                     /locus_tag="PLD98_01350"
     CDS             complement(17961..18536)
                     /gene="hisB"
                     /locus_tag="PLD98_01350"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HQA04931.1"
                     /translation="MRKKTITRTTNETDIGLTINLDGEGMAEVNTGCGFMDHMLTLFA
                     FHGGFNLSLACKGDTKVDYHHTAEDVGIVLGKAIRECLSGGINRYGSIILPMDEALVL
                     IALDVSGRPYLAFDAQIPSQKVGVFDTELVEEFMRAFSNNLGCTLHIKQLSGKNSHHI
                     IEAMFKGLGRALKEAVRETGGGEASTKGILL"
     gene            complement(18533..19594)
                     /gene="hisC"
                     /locus_tag="PLD98_01355"
     CDS             complement(18533..19594)
                     /gene="hisC"
                     /locus_tag="PLD98_01355"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQA04932.1"
                     /translation="MGKFLSPRLKGLKPYTPGEQPQGGKYIKLNTNESPFPPSPYVFK
                     ALEGQAETLRLYPDPENRLLNEAIAENFGLSPENIIAGNGSDELLALIFQAFCPKGAA
                     FADITYGFYKVWAEFYGIDYKIVTLKDDFTLCLEDYRDLDETIVIANPNAPTGLAVSP
                     EKIAALAAEKPDRLIVVDEAYVDFGAESCIRLVKELKNLIVVQTFSKSRSLAGGRAAF
                     AAGDAELIRDFNTVKFSFHPYNLDRLTQLAAAAAIKDRVYFETCTSKIIKTRERVSGE
                     LKTLGFFLTDSMTNFIFARHEKIPGAEFYRLLKEKGVLVRYFGTERIEDFIRITVGSD
                     DEMDAFLKALKDILKEAAK"
     gene            complement(19587..20855)
                     /gene="hisD"
                     /locus_tag="PLD98_01360"
     CDS             complement(19587..20855)
                     /gene="hisD"
                     /locus_tag="PLD98_01360"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQA04933.1"
                     /translation="MIKIAEYDSKAFKRERAQADADVRYIINDVAERGDEALFEYTLR
                     FDGCKPESLLVTQEEIDAAAESVGEYFLDTLKMAAENIRAFHKKQLSDGFSMTAPFGS
                     ELGQIVRPIEKAGLYIPGGTASYPSTVLMNAIPAKIAGVSEIVAVTPPDKEGRVNPAV
                     LAAAKVAGIDKIFKVGGAQAIAALACGTESIPKVDKIVGPGNRYVASAKRLVYGLVDI
                     DMIAGPSEILIVADKNADPVNIAADLLSQAEHDTLASAILVTDNKELAEKVQAEVERQ
                     LELLPRAEIARQSIDNQSAIFLCSSISEAIEIANDIAPEHLEICVEEPKKYLADIKNA
                     GSIFLGAYTPEALGDYFAGTNHTLPTSGTARFASPLGVDDFIKRSSYIYYTKEALSEV
                     KDRIAHFARFEGLEAHARSVESRFRGEGNG"
     gene            complement(20852..21472)
                     /gene="hisG"
                     /locus_tag="PLD98_01365"
     CDS             complement(20852..21472)
                     /gene="hisG"
                     /locus_tag="PLD98_01365"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQA04934.1"
                     /translation="MLSIALPKGRLGDDVYDIMKNAGFAEGDINNMGRKLVIENKEAG
                     LRYFLVKPSDVAIYVEHGAADLGIVGKDILEEDRADVYELLDLGIGKCRMCVAGPKDF
                     VDDGSRTLRVATKFVNIAEQYYESLGREVDIIKLGGSIELAPLLGLSDVIVDIVETGK
                     TLKENNLDVLAEIMPISARLIANKVTYQFQSSNIDRICEKMTEVIK"
     gene            complement(21465..22385)
                     /locus_tag="PLD98_01370"
     CDS             complement(21465..22385)
                     /locus_tag="PLD98_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQA04935.1"
                     /translation="MENLLDILSPQERTVLSFRALFESKGYRRIKANRFEEYRFYLEN
                     INFLRSEQLITFSDLDGRLRALKPDVTLSIVKHYGSNGGLNKLYYIENVYRPGPTGRS
                     FAEISQMGLECIGKVGKAEVSEVLYLSYESLKLTGKPFRLSVSHVGFIAGLMKSLKVP
                     TQYRGALYESIASKNPLGLRMTAEAAGLSGENIDFLQKAALLSGGADKVIDTAFKYCK
                     NSEMHRAVGQIKDAIDGLDSSNILIDFSIRGDEDYYSGLMFCGYIKGRSRVVLSGGQY
                     DNMLKKMNKEGRAIGFALYLSELFGGERHA"
     gene            22661..23155
                     /locus_tag="PLD98_01375"
     CDS             22661..23155
                     /locus_tag="PLD98_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQA04936.1"
                     /translation="MQSSLLRTNEELTEIYSRHVDTVYRVCFMFMKNGPDTEDMVQNT
                     FIRLMRDNTVFQSIEHEKAWLIRTATNLCKDHLKHWWQKRRAGMDEAEKLESEPAFEA
                     DNNLEMVLNLPPKYKTAVYLYYYEGYKTAEIAKMLGKNESTIRGWLYKSRQLLKLEME
                     GDLQ"
     gene            23152..24345
                     /locus_tag="PLD98_01380"
     CDS             23152..24345
                     /locus_tag="PLD98_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04937.1"
                     /translation="MRKEDLKASLDKIKPDEAAKRRMRYNIMNYNEEKTARPILWKRA
                     VPALALVLVLTAGIFAYDKIFSEANTYKPPVLSSEADDYAREDMAAPIVNQFKFEDRD
                     YIILSDELRKEFGLPSEIRDGDIGDKLGTITQGPDEKLIGKEVYEYIPADCEAIVAVK
                     DGDSYKLFRFFNFESYKNNIDEDAERYLKLFGIKSAEDISKVQFILYSEEAKLRGGFD
                     IKAELKGKDEIKKFYDFYSGLKNSSDEYFKALYNYRREGIDHGKEVYPPVPDTAVSQI
                     APDAPNASSPVFPDGAEPAYYADDLPAAVPERANGAATPANDGNLSTVVGHRGMMDIG
                     ETGVETVTANQGSAGAALENAVTIRIYNKNGIYYDTIYYPNIGFISRYKLGDNFKAFL
                     NSYVK"
     gene            24472..24819
                     /locus_tag="PLD98_01385"
     CDS             24472..24819
                     /locus_tag="PLD98_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQA04938.1"
                     /translation="MPEKVNLKEAVNSLTELFEYKKVGYFNNQMMNVLIAENRTLDYH
                     VHENSEEVFYCIEGEFDIEFDDGPVHLAEGDFIIVPRGTRHRPVCKGLVKCLLIENEG
                     TLNAENTGGAYKP"
     gene            24949..25113
                     /locus_tag="PLD98_01390"
     CDS             24949..25113
                     /locus_tag="PLD98_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HQA04939.1"
                     /translation="MKKYICIPCGYIYDPEVGDIDGGIAPGTAFEDIPDDWQCPICFV
                     GKDEFEVFED"
     gene            25128..>25442
                     /locus_tag="PLD98_01395"
     CDS             25128..>25442
                     /locus_tag="PLD98_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HQA04940.1"
                     /translation="MSMFCFQCQETAGGKGCTVRGVCGKTEEVAKLQDLLIYTLKGLS
                     EVVVKGGFNAKDLGGINYQLLNSLFMTITNANFDPEAIEKQIVKMLSVRDELRKKSDK
                     ADF"
BASE COUNT         6602 a         6241 c         5869 g         6730 t
ORIGIN      
        1 gctgcggaca gactgagcca ctttgtagtg ttccgtgccc acaatatgag gctccagaat
       61 tcttgaggag gagtcaaggg gagcgacggc agggtagatg ccctgctcca caatcgacct
      121 tgaaagcacc gttaccgcgt caagatgggc gaaggttgtg gcggtggagg ggtcggtaaa
      181 gtcgtcggcg ggaacataga ttgcctgaac cgaggttatg gaccccgact ttgtggaggc
      241 gattctctcc tgcaaagcgc cgatttcgtt cgccaaagtt ggctgatagc ccactgcgct
      301 gggagtgcgt cccaaaagcg ccgaaacctc ggaacccgcc tgcacgaacc tgaagatatt
      361 gtctatgaac aaaagcacat cctgctgttt ttcatctctg aaatactcag ccattgtaag
      421 acccgtaagg gcgaccctca ttctgactcc cgggggctcg ttcatctgac cgaatacaag
      481 ggcggtgttg ctcagtaccc cggaatcctt catgtcaaag taaaggtcgt gaccttctct
      541 tgtgcgttcg ccgacgccga caaagaccga gtagccgccg tgctccttag cgatgctgtt
      601 tataagctcc ataatgagta cggttttgcc aacgcccgcg ccgccgaaaa gtcctatctt
      661 tccgcccttg ggaaaggggc agagcaggtc gatcgcctta atgcccgttt caagcatttc
      721 cggctgagct atctgggctg cgagtttagg cggttccctg tgaatctgcc aatagtcctt
      781 agcctcaatt tccggcagat tgtcaatggg cttgcccaaa acatttacca ttctgcccag
      841 gacctcctcg ccaacgggaa ccttgatggg ggagcctgtg tcgtaagcct ccatgcccct
      901 tctcagcccg tcggtggagt ccatggaaat gcagcgcacc gtcctgtcgc caaggtgctg
      961 cataacctca aggactatcg tcctatcctc atggggaact tctaccgcgt tatggggaac
     1021 ttctaccgcg ttatatagtg agggcaggcc gtcttcaaaa cgaatgtcga cgaccgctcc
     1081 gataatctga gttattctgc cagtgttttt cttcattcat tccacccttt aaagcaaact
     1141 cttgctgcca actatctctg tcagctcctg agtgatggca gcctgacgct tgcggttgta
     1201 ctgataggta agtttatcaa tcatctcctc ggcgtttttg cttgccgagt ccatgctgac
     1261 cattcttgat gcctgctcgc tggtatggga ctcggaaaac gccctgaata tactcatgtg
     1321 aatataaagg ggaatcgtat gttcaatata tgtcatcaaa tcgggctcgt actttctcaa
     1381 ttcctcgtag tattcacgag gactgtaggg caggggaagc agcttttcca cggtggggat
     1441 gttctttaaa acattttcaa aatgggtgta gcagacaaaa acctcctgca ccttgcccga
     1501 aagaaaaagg ttcagaacaa actttgagat gtcttcggtc tcgtgataca ttcgggcgtc
     1561 ggaaacatcg gtaatcttgc atgctatatt aaagtccctg cgactaaaaa actcatagcc
     1621 cttttgccct atggcgatta tctcagcatt cttgccgccc gcctcaatat gctccagaac
     1681 cttctgggtg atgttgtggt tataggctcc cgcaaagcct ctgtcgctcg tccttacaag
     1741 gtacagagat ttttttacct ctgagctttt gtaaaaggga tgcttggaag cctcctcatg
     1801 gtagcctatt tcctctgaga ttctttttag cccctgataa atcgggcgcg cgccctcaag
     1861 ctgagccctc gccttatgga gctttgtgga ggcgataata tccatggcgc tgataagctg
     1921 ctctacggat ttgacattgt ttattctttg ctttatgcct ttaagcgatg ccatatcacg
     1981 cctccttagc taaagtagtg tttgctcttt acctcgctta taattgaatc cagtttcttt
     2041 gcgttctggt cggatatttc ccccgtattt ctgatttctt ccaaaattgc aggctcttgt
     2101 gagctgacgc ttttaataag ctcgttctgg aagtgctgta tgcgccatgt gtctatgtca
     2161 tcaagatgcc tgttgtttaa agcgtataga atcaacacct gattttccac aggcatcggc
     2221 ttgtaattcg gctgctttaa aacctcggta attttttcgc cgtgattgag acgcatcagc
     2281 gtgtctttgc ttaaatccga gccgaactgg gtaaaggcga ccagctcctt gtactgggcg
     2341 aactcgattc tgagaggacc cgccagtttt ttcataatgg gtatctgagc cgcgccgccg
     2401 actctcgaaa ccgaaaagcc ggggttgatg gcggggcgga tgccgtggtt gaaaagctcc
     2461 gcctcgagat atatctgacc gtcggtgatt gaaattatat tcgtagggat atacgcggaa
     2521 atatcgcctt cctgggtttc cacaatcggc aatgcggtaa ggcttcctcc gccgtgctct
     2581 tcgctcagag cggcagctct ttcaagcagc cttgagtgga gatagaaaac atctccgggg
     2641 aaggcctctc tgccgggcgg gcggcgtaga agcagactta tagccctata cgccaccgcg
     2701 tgctttgaca ggtcgtcaaa gactatcagc gcgtccttgc ccttatacat ccaatgctcc
     2761 gctattgcac agcccgcata gggcgcaagg tactggagcg acgccgagtc gctggctgtg
     2821 gcgataacaa cggtggtata ttccgtggct cccgtttcag tgagcgcctt tataatcctt
     2881 gccattgtgg acgccttctg acctatggcc acatagacgc agaaaacatc cttgcccttt
     2941 tgatttatta tcgcgtcaag ggcgatagag gtcttgcccg tctcgcggtc gcctataatc
     3001 agctccctct gaccctttcc gatgggcaca agagcgtcga ttgccttaat gcctgtctgg
     3061 aggggctgat ttaccgcttt tctcataata acgctgggag cggggctttc gattttgcgg
     3121 taggtttcag cggcgattgc gcccctgccg tcaatcggct cacccagagc gtttacgact
     3181 ctgcccaaaa gagcgtcgcc cacgggaacc tctgccattc tgcctgtgag ctttacggga
     3241 tcgccctcct tgatgcctgc atcggagccc ataatgatga ctccgatgct gtcctcctca
     3301 aggttcagag ccattccgta tgtgccgttt gagaattcca gcagttcgcc gctcatgaca
     3361 ttgccgagtc cgtataccct tgcaatgccg tcgcccacct gtatgacagt tccggcctcg
     3421 gtaaaatcag ctaccgaaga gtaagactca atctgctttt ttattacgct gcttatctcg
     3481 gcaggatttg ctgtcatgcc aatatttcct ttctttaagt attctttcat tctgataagc
     3541 tcggttctga cggtgcagtc aaaaacacgc ccgtctgcaa gtatataaaa tccccctaaa
     3601 acatcagggt ccaccgagca ttccagctct acctgtctgt ttgtctttga gctcagcaga
     3661 tttcgtataa gctctctttg cttttcgcta agctcctttg cggaaaccag ctttgccttg
     3721 agcctgcctg tgctcctgct gagcagctct atgtactcct caagaatggg aagaataagg
     3781 ctttctctgc ttttttcaac catcagcctt aaaaagccct ttaagtgttc gggagcgtca
     3841 tctgaagccg ctgtttgaag aaccgacagc ttttccctgt cgggaacatt aggataggtc
     3901 aaaaaagcct ttgtttgagg gtcgcttata ctctctttta ataaagcagc ctgctttaaa
     3961 tccctttcaa aactgccttt ttcccttgaa agctctaaaa gagcctcggc ataacgattt
     4021 gtcagctttg ccatttcgat aactccagtt ttgaaatagc ttcctcaaac agctgttcct
     4081 tagcattgtc gtcaatgctg ctttcaatgt atttctgcgc taaatttacg gcaagctcaa
     4141 ttatgtaaag gcggctttct tcccgaagtt ttattttgtc aaattcaatt ttttcctgcg
     4201 cgcgctttaa aatagcctcg gcctcatgct ttgcgttttc tacgattgcc cttccttcct
     4261 cggcggcctt cagacgggcg tcttcaagaa tcttttccgc ctcgtcgtct atgtctttga
     4321 gccttgcctc aaactcggcc acaagagcct ccgccttttg ctttgccttt tcggcgtctt
     4381 ccatttgacc ctttatcttc aaagagcgct tttccatgta ttcttttacg ggcttataca
     4441 gtatgaagga gagcgcgccc gttagaatca gcacgtttac aaactgtatc aaagcaccga
     4501 taagggtctg ggagtcaagc ccgaaaagcc tatcctctac ggcggcctgg gcaagcagta
     4561 tggtaaagtt caagcattca gcctcctttc actaaggtta ggtgagcagg tttacaaagg
     4621 tgtttataag cggatttgca aagagcatga taatcgcgat gagaagacca tatatacccg
     4681 atgtttcagc catcgcaagg cctatgaaca tggatgtgcg gatattgtcg gctgcctcag
     4741 gctgccttgc catggactct attgcctgag aagcgatttt tgcctgtccg aatgtggtga
     4801 gaagtccgtt taaaagacag attgcagccg ctatatgcgc gacagagata ataagtccca
     4861 atgtttccat aagtaaattc ctcctgtttt ttatgtgaat ggcttaatca gccgcatttt
     4921 tgacaaacat aaggctaacg accacaaata tataggtctg caaagctccg aatatgacat
     4981 cgaaaaccgc gtgaagcaga gcgggaattc ctatctggac aaaaatcgga gtcagagcat
     5041 agtaaagggt cagaatgatg gtgcccgaaa gcatatttcc gaaaagtcga aagctgagtg
     5101 aaataggctt tgacagctct ccgattaaat tcagcgggaa aaatatcggg tacggctcaa
     5161 aaaagctctt taggtacttg ccctttttgt gcttaaagcc cattatcagc atcataagaa
     5221 agctaagcat agcaagagca aaagtggtgg taaagtcggc ggtcggcgct ctcagaccaa
     5281 aaacgctgaa gagagcggat gatagaataa aggcgaagac agaaaagaaa agtggcgata
     5341 tgtacgacag cttttcgccc aaattgcttt tggcaaagcc gtcaaaaata tcaaccgccg
     5401 cttcaatgaa gttctgtatt ccctcgggta tctctttaaa agagcgcagc ttgattctcg
     5461 cgaaaatcgc gagggctatg agacccagca ttataatcca ggtgttgacg atggtttccg
     5521 ttatccaaac ttcaacgccg aatatgttaa atgtccaaaa ggtctttacg cctatgccca
     5581 ataagttcac cccttaagag tctcttttgc cgaatttaac cgtataagtg gctatctgaa
     5641 aggagagcac tcccaccgcc gtgccccaaa tgctgatacc gggcgacacg gctccaacat
     5701 aaagcactat gcccgtaata aagagcctta aaatttgctg cagggcaaca tagccgccag
     5761 cgcttttttt gtccattgaa agacttttgc tcacagctct ttcaaggaga aataccttgc
     5821 ccacggccgc ggcggagcct atcagcgcgc cgtacaaaaa gggcaggaat gatagcgacc
     5881 tgtggtaaat tgcggctatc agcactgcgg cggagtttat tatcagtatt gaaatcagca
     5941 ttcgctttgc caatgcggaa attttcataa tttcaaccta tttttctaat ttagaaaaat
     6001 caaagacaaa tttaatggct gccccgactc caagcagaga aaaaacaagt aggagaaaag
     6061 gagcagtacc gaggagatta tcaagcgtct ttcccagaaa gacgccgaca aagaccgagg
     6121 cggcaattga aaaaccgatg tttgaaaaat cggctaatgc cttaaaaaag cttgtgccct
     6181 ttgacctatt gtccttatca tcctttttgt ccaaagcagc ctccaaactg ttggatgttt
     6241 tgctattatt ccccggcagt gaatagtgct gcctaataaa agcctaacat aaacttttgc
     6301 cctcatgcaa gttcagaaaa aataatttaa agttttttta caaagtttta agcgatttcc
     6361 acatggagtt cgcataaatt ggaaaaatat ttgtagaaaa tgacaaatat tttcaaaaaa
     6421 tggtatgctc ggtttgcaca aaatcaaaaa tgaaggacct ttttgggctg attctcacct
     6481 tattcacgat ttgttaaaat tgcgtacaag ttatattaac aatttgttaa agccttgact
     6541 gcgctgggat ttggattttt atatttttaa aagctaaagc aaagattggc gtttttatac
     6601 atttcccgca catatttact tattatgtat gatatttatt aaaattgatt ctaaattgga
     6661 aagtttttgt gcaaaaatac ttgtattgtt cagaacacga tgctataatt aagttgcttt
     6721 actgcaagaa aatattttaa ggagcgatta gtttgagaat cagaaagcaa atctccattc
     6781 ttttggctct tgttatagct ctttctatgc tcggcgtatt tggcgttgca tgctccaagc
     6841 ccagcgtaga ggacataaac aaggtcacca ccgctaatgt atttgaagca attaaagacg
     6901 catacaaaga tgtaaccggt aaagagatgc ccaaagaggt tgccgacaag cttaacgacg
     6961 acctttacag caagatagct cagggcacag gctacatgga gtatgccccc tatgcaagtg
     7021 agttcattta tgaaaagaca ggagttatca ttcctcccga aacacttgca atagcaatca
     7081 aagcagcggt tttggttgcc ggcaatctgt aatcggttga aatttacccc cttgcagtag
     7141 agcatcggat caagcctgtt cataatgaac aggtgaatcc tgtaataaag ggaagataac
     7201 cgctgcggta tttgccgtgc ggttatcttc tatctttaat tggagccctc ggaggaaaaa
     7261 tatgtatccc tacatggaaa tattcggccg cctcattccc tcctatcttg ttatggcgct
     7321 gattggcctc gtcttcaccc ttgttctggc actcattcgc agaaaaaagg ggaacttaaa
     7381 tttccaggcg gaagatgtaa ttcttatgat tctcatagca gctatcggcg ccatgctggg
     7441 agccaaggct tttcagcttg tgggcgtgat tctgcgtgac ggaaatgagc cgggcttttg
     7501 gacttgggag cactggaaaa gccttattcc caccgccggt gtgttctacg gaggttttat
     7561 cggcggtttt gcggctatgc tgatatatat cagaaagaac aaattcgatt tctggaaggt
     7621 tgccgacctt atggtgccaa gcatcctgct tttctgcacc ttcgggcgca taggctgctt
     7681 tcttgctggc tgctgctacg gcaaggttag ccacacctgc ggtatagtcc ccctttacgg
     7741 cgcagagcct ttaattcccg tacagcttta tgagtctgcc tttacccttg ttctgctgat
     7801 tggcctatta attacacgcc ccgaaagaaa acgagagggt atcctcctgc ccatatatat
     7861 tctgagctac gccgccgcgc gctttgcgct cgagtttttc aggggcgata tgcaaagggg
     7921 agttttcatt ctctcaacct cccagtggat ttcgcttctc atggtgcctt tgggaatata
     7981 cctgcttctc agaaacaaaa agcacaacga gaaaccaccc gccgaatcga atgagtaaac
     8041 ccagcaccgc ttagtctgtg ctttttatgc ttttaaaggc agaagaaata aaaaggcggc
     8101 atcattggat gccgcttaat ttattcaagg caggcgctga gcgtcttgcc tatgctgtcg
     8161 ttaataacaa gttttgccaa gttgtcatag ggcgtggggc ttttgtttat cagaaccagg
     8221 cggtttccgt tatagtacct tatcagcccc gcggcggggt agacagtgag tgaagtgccc
     8281 gccacaatca gcatatccgc tttttcaatg tagtttacag ccctgcccag tatatccata
     8341 tcaaggcttt cctcatacaa aaccacatcg ggcttgacta ttccgccgca cgcatcgcat
     8401 ttcggcactc cctcagagct taatatgtaa tcaaggctga agaactttcc gcaatcaaga
     8461 cagtaatttc tgtgaaccga gccgtggagc tccaaaacct ctttgctgcc cgccagctga
     8521 tgcagaccgt cgatattctg cgttatcacg gctttcagtt ttcccatatc ctcaagtctt
     8581 gccaaagcaa tatgggcgct gtttgggcgg gcgtccttgt atatcatctt ggatttatag
     8641 aactcaaaaa actcctcggg gtaccttgcg aaaaaggaat gggacagcat ttgctcggga
     8701 gcaaagcctc ttttgcctcc cttttctgag taaagtcccg tcgcgcttct gaaatcgggt
     8761 atattgctct ccgtagaaac tcccgcgccg ccgaaaaaca caatattgtc tgagaactca
     8821 atgtattttt taagttccga gattttgtcc acaaaactca cctcactcat ttccccctat
     8881 ataataccac ttttttactg ccgcggcgca aatctatttc gcaaatattg ataatattca
     8941 ccttaagttt tattataata ttaaaacacc ttaaagagtg agggcttatg ttttttgtgg
     9001 cgtttttcgg aatgaacgac aaagaggagc atataggcgc tttcagcgga gagggctgcc
     9061 cctcctgcgg acagctttca caatttgcgg tcagcaagac ctacagaaac ttagaggttt
     9121 tctttttacc cgtttataag tggaatgtaa gatacatcgc gagggcgctt tgctgcggcg
     9181 ctgtttttga gcttgaccct attctgggca aagagcttga gaaaaacccc tatataaaaa
     9241 tcagacccga aaacttaagg cgcatagaaa gacaatctcc ctttaaatac tgcccgaact
     9301 gcagagtgga tgtgccgaat gaatttgcct actgcccata ctgcggagga aagctgtaaa
     9361 agctgaggta aaaagacctc agctttttat tcttctgcca aagcattgtt ttttacagtt
     9421 tgagctgaac attggcgttt gagcagatat tgcctgcctc ccaaaaaaga ccgcttgcat
     9481 tattcacttc ggtgctttat aataagggca aataacatat attggaaaag agggttctta
     9541 tgctgcttga ccatgtgaag aagtattttg cgttcatttg gagccttctg gttctgtttg
     9601 cgggactctt tacaagaacc gttgcgcccg ttgacgcccc gaaccttgag gtagtcagca
     9661 attaccacta cataatggac gaagctctgg ttatgggaca gggccttgcg aatgacggca
     9721 catatcttta ttcctcagga gcgatttccg ctttgtatct cggcgctctt tccaaaatcg
     9781 atatgaatac gggcgagatt cttttaagga aaatcaacgc catccccgag gagtttgtca
     9841 agaagggcta tgaccacatc ggcgacataa gctggcataa cggtctaata tacgctcccg
     9901 ttgaggattt acaggaagaa cagccccttg tgctcctcta tgacgccgaa actctcgaat
     9961 acacgggcac atatttcgaa ttggacaaca cctatctgcc cgacggaatt ccgtggtgca
    10021 cagttgacgc cgacaacggc tatctctacg cctcaccctt taaaaatgta acctgcatcg
    10081 tggcatttaa cctctccgat atgagctttt cgcacattat tcccttaagc gaagaaataa
    10141 caagggttca ggcagccgac tattttgacg gaaagctcta tgtaaacctt gatacccaag
    10201 gcaccggata caaggaaata aaggtcatag atgtaaatac cggcgaggtc agccttttgg
    10261 cgcaaaggga tatgcagggc cgcgatgtgg aaaccgaagg tcttactatc accagggacg
    10321 aaaacggaaa gctcctgttc cacattacgg actacgacaa gactgtgtcg gtatatttaa
    10381 gaacatacag actcactgac taagataaaa gccgatgaag tacgcttcat cggctttttc
    10441 tgtaaagcgg agggacatat gaaaatcaga agatatataa agcaatacga tgaagaaaat
    10501 gtaatggaaa tgctgaaaga agagggagac gaatggcgct gctaccacgc ggatgaagtg
    10561 agcgcaaaat acaaagccgc ccttcaaaac tccattacct atgtggcgta cgaaaacgat
    10621 gaaatctgcg gctattcccg ctcccttgac gactgcggct tttatatcta tgtctgcgac
    10681 ttgctggtaa gaaaaaatac aggggcatgg aaataggcag aaagctcatg gagtgcatat
    10741 acaaagactt ccccgacaga atcgtctatg tcatgtccga cgccgacggc tattatgtaa
    10801 agcttggcta caggcgtgaa ggctccgttt ttgaagttac gaaagaggag taagctcaaa
    10861 gaccgctcat acgggcggtc tttttatttt ttcggaaaaa ctataaaaag tgttgacatt
    10921 gacgcagcgt aatagtttac cctatatgat gaggagggga gagcatggaa tacaccgtaa
    10981 gccgactcta aaaatggcgg gcgtcacccc aagaacgctg agatactacg accaaatcgg
    11041 actattaagc ccaaaaagaa cagactccaa cgactataga gtctacggca cagaggatgt
    11101 tgaccgcctt cagcaaatcc ttttttacag ggagctgggc gtgtcccttg aggatataag
    11161 gaaaattctc acgggcgagg actttgacga actatcagct cttcaaagtc acctgagcgc
    11221 tttgcttgaa aggcgggagc agctgaatct gctgataagc aatcttgaaa aaaaccatta
    11281 aagcgaaaaa aggagaaata tttatgagcg ataaagagaa atttgaaggc tttgtgaaaa
    11341 agctggtaga ggaaaacgaa gaaaagtacg gaaaggaagc aagggaaaag tacggcgacg
    11401 aggctgtgga ccgctcaaac gcgaaactct taaatctcag ccccgaaaag tacgccgagt
    11461 ttgagaagct gacaacggag ctgaacgaaa ccttaaaggc ggctgttgag cagggtgacc
    11521 cgagcagcga aacggctcaa agggaatgcg agctgcataa aaagtggctg tgctttttct
    11581 gggacagcta cagcaaggag gctcacatgg gcgtaactca gatgtatgtt gacgaccccc
    11641 gctttaaaga gtattacgag aaaatcgccc ccggctgcgc agaattctta agagacgcgg
    11701 taagcatcta ctgcgaaaaa cagtaataaa cgcccccaaa acaaggttag cggcaataaa
    11761 cttaaagcat taaaaccctc tccgctattg cggagggggt tttgcataga aagccgttat
    11821 tgacaaaagg gaaaataaat gccaccatta aaccagatgg tctaaaccaa tcggtctaat
    11881 atatctcggc actttgatac ttcggaaggg tgtttgcgga ttatatcatc aagacggaaa
    11941 aaactactta agccgttgcg gcggctataa ataggacatt aagccgcaag cgttgcccgg
    12001 tataccgccg ggcaggttgt caataaaggg ggtgtaaaca ttgcacttat tattgttatt
    12061 attatttata ctggcattgc ctatcatgat aatacttgac tgcgctaaac ggtcataaac
    12121 tcaagaagaa ccgcctataa cgggcggttt ttctattgcc tataatatcc cacgaacatc
    12181 ggaaagccgc cgaaacggta tgaaaagagg ctttgattac agttctctca taggcagcgg
    12241 caaccctcta aaaagctatc ctcagcttta tgaggccgca gtcaatgagt tttcaatgaa
    12301 aaaatacgag gaagcgtccc tcaatgatat tcttaaaaac tccggcatga gcaagggcag
    12361 cttttactat catttcggcg acaagttcgg gctgtatctt gccatgatgg atataataac
    12421 aaacaaaaaa ctctcatact tttatcccgc ggttcaagaa agggcgggcg agagcggctt
    12481 tttcgatact ttgaaagaca tcatgaaggg caccatggac tttatgctgg cagacgagcg
    12541 gctttatcac cttcttaaca gggttttgga ggaggatgaa agcctgcgca gccgcatggc
    12601 agagtttttc cttatgacaa cgaaagattc ttcagcgaat atataaaaag ggcaaaggaa
    12661 agcggagaga tagactcaag ctgcgacgag gattttattg caaaactcat tatgataatc
    12721 ttttcaaaca ttcacaagct tattgaaaac aagactccaa atgagctgtt aaaaaccgca
    12781 aatcaggttt tggacataat tcggttcggc atttcaagat cggcagaggg ggaatagttt
    12841 tgtcagttgc gttcagtgtt caggatttac gctttaaata cccgtcggca aaggaggata
    12901 ccctgaaggg catatccttt gaggtaaaag agggggagat tttcggactg ctgggcccgt
    12961 cgggcgcggg caaatccacc acgcaaaaaa tacttataaa gctccttgag gattataagg
    13021 gcagcgtaaa atacttcggc aaagacctta aagatttggg caagtcctat tatgaggaaa
    13081 tcggcgtcgg ctttgaggtg ccggtccatt tttcaaagct gaccgctctg gagaatttaa
    13141 agttctactc gggcttttat aaaagtaaag ccgacattag ggagcttatg gaaagggtag
    13201 gactttggga gtaccgtgat aaaaaggtgg gcgagttctc aaagggaatg aaggtgcgcc
    13261 ttaatttcgt ccgtgctctg cttaacaagc cccgtgtgct ctttctcgat gaggtcacaa
    13321 acggtcttga cccaaagaac gctagaatta taaaggatat gatagccgaa taccgaaaag
    13381 acggcggaac ggtctttttg tccacccatc ttatgaacga tgtggagcag ctttgcgaca
    13441 gaatagcatt ctgcgtaaac ggcagtcttg ttgaggtttc gaccccaagg gaattaaagc
    13501 tcaaatacgg caaaagggaa gtcaaggttg agtataacga aaacggcaat ctttcaaccg
    13561 ctgttttctc ccttgacgga atcggcgaaa acgaggagtt tgtaaacctc ttgaaatcca
    13621 agcaaataga aacaatccac agcggagaaa cctccatgga ggaaatcttt attatcgtga
    13681 cgggagttga gctgaatgag cgtactgaag ctgtttagcg gcgaaataat caggctcgta
    13741 aaatataaaa tcctccccgt cggcgtagcc acgacggcaa tatgggtggc tattttgtat
    13801 tttcttgagc ctcgggagga gctgtatctt gccccgatgt ttatattcat agatgtgggc
    13861 gccatgtcga ttctgctgat tggagcctcc cactttctcg aaaagcagga gggcacaata
    13921 aagagcctga tggtcatgcc tgttacaaag agggagattt taatctcaaa aaccgccgcc
    13981 tccatggttt tggctttgga atctgctgtg ataaccgccg ctgcgctcta ttttatacac
    14041 aagataacct taaactacgc ccttctcttt ctgttcgtca ttatagctgg cgctgcccat
    14101 gcggcaatcg gttttgtgct gtcgctaata ggcagggatt ttacctcaat gctgggaatc
    14161 atggcggcat atatgtttat cttcaccata ccgtcaatat tttacagcgt ggggctgata
    14221 gatgaaaaat acaaatacct tatgatggtc tcaccctccc acgcttcaaa cgagcttata
    14281 agagccgcag tgagcgggga gtatgagacg gggcttatta tcgcgggctg cgtatatctt
    14341 atacttcttg ccgcggttct tttcagattc gcggtatatc ccatgttcaa agacaaagcg
    14401 gtaagggggt aaaaaagtga agaaatatct atggctttta aagtacgaag ctaaaaccat
    14461 catccgagag cctgtaaatg tctatatggc tctgtccccc ctgcttgtgc tgtttttatc
    14521 tgtatttgta atgcccatgg catttaactc aatcgacccc gcaaatcaca cagtgcttaa
    14581 aaccacaatg ctttttatga ttatattgct gcttgcggtg ggctcatctt tgcttgccgc
    14641 aatggcgaca ttcttgctgc ttgagcacaa ggacgaaaac acacttaaaa ccattgcagt
    14701 cacccccacg ggagcatcgg gctatctaag atttaagctg acatatgtgt atgttatgtc
    14761 ttttctgggc attatcatgg tgcttctcgg cacaaagctc attgcgggcg acaagtactc
    14821 aataaacggc gtttcgatgt ttgacagcgt aaatgtatat cacatcattg cctttgccgc
    14881 agtggacagc atttttgccc cctttcttgc gctgctgcag tccgccttcg ctaaaaataa
    14941 ggtagaaggc tttgcgtata tcaagggttc gggcatagtg gtgctgatac ccgcgcttat
    15001 gctgctgaaa acttttcagg gttcgcttca gtatgcgctg ggagttttcc cgaatttctg
    15061 ggcaataaaa gcaatgatgc tgagatttat gcccgccgac agcggggcga acctcagctt
    15121 tcccgtatac ttaattattg gtcttgcgta caatattctg ctgcttatag tgtcatacag
    15181 actgttcttg aaaaaggctc agtactaaaa agaaaacccc tgcgaggttt ggcgggggtt
    15241 atttcattgt ttcctgtttt gtctttttgt caatcacatg gcgggaggca agctccgctc
    15301 ttatgtcgtt aagagagata cccatttcag ccataagcac cattacatga taggcaaggt
    15361 cggcaatttc gtatatagtt tcctccttgc tttttttcat ggcgccgata ataacctcgc
    15421 ttgtttcctc accgaccttt ttgagtatct tttcaagacc cttgtcaaaa aggtaggttg
    15481 tatagctgcc ctcttttgga ttttcctttc tgccttttat tagctcataa agcccctcgt
    15541 aggtaaaaac atcggcgcca tcatgaatct cattgaaaaa gcagctctct tcccccgtat
    15601 ggcaggctgg accggccggc tccacgtcta ttagcagaca gtcgccgtcg cagtcagtct
    15661 ttatagatac aaccttttga acattgccgc tggtttcgcc ctttctccaa agctccctgc
    15721 ggctccttga ccaaaagcag gcgcggccct ctttttttgt tatctcaagg ctctcctcat
    15781 tcatatatgc catcataagg accttgcccg ttctcgcgtc ctgcactatg gccgggatga
    15841 gccccttttc atcgaattta agattcataa agtccctccg ttcggacatt cactccgtgt
    15901 tcttttaggt aaagtttcag ctctttgatg ctgacctctt tagtatggaa aattcccgcg
    15961 gcgagcccgg cgtctattct gtcgtattta aacagctcgg cgaaatcctc catttttccc
    16021 gcgccgccgc tggcgattaa aggtatggac acaatctcac cgatggctct cagcataggc
    16081 atatcaaagc cgcctcgcac cccgtcggtg tctatggagt ttaatactat ctcgccagcg
    16141 cccagagcct cgccccgctt tgcccactca agagcgtcaa gacccgtatc ctccctgccg
    16201 cccttggcaa agactctata ggagccgccg accctcttta catccatgct cagcaccacg
    16261 cattgactgc cgtagcgctt tgccgcctcg ctgataaggt cggggttttt aatagcgccg
    16321 ctgttgacgc ttaccttgtc ggcgccgcat ttgagcaccg tgtcaaaatc ctcaagggag
    16381 ttgattcctc cgccgacggt caggggaata aaaatctccg acgccaccgc ccgcagaaca
    16441 tcggtaaaga gctttctgcc ctcggcgctt gcggtaatgt cgtaaaagac cagctcatcg
    16501 gcgcccgatt cgttatagta ccttgccatc tccaccgggt ccgccatgtc ccgtatgccc
    16561 tcaaaattta ctccttttac aaccctgccg tttctcacat caaggcaggg aattattctt
    16621 ttgctgacca tctgttagcc tccgaattta tgtataaccg tttttaaatc cagggctccg
    16681 ctgtaaagag ccttgcccag aatcgcgccc catgcgccga ttttctccag tttctcaagt
    16741 tcctccatcg tgctgattcc gccgcttgcg ataatgttta tacctatttc gcagagcttt
    16801 tcataagcgg cgagattggt gccctttgaa gctccgtcgc atgcgatgtc ggtgtaaata
    16861 acggtttgaa caccgatttc tttaagacgg cggacgaact cgaaagaatc cacgctgctt
    16921 gtttcaagcc agccgctgac agccacaaag ccttcacggg cgtcaacgcc cactgcgatg
    16981 tgcctgccgt atttctttac catctcggcg gtaaactcaa aattctttaa agcggcggtg
    17041 ccaagtataa ccctccgggc ccccgcctca aggtatttta atattctctc ctcggtgcgt
    17101 attccgccgc ccacttctac tttaagaccg ctgtatttca gaatgtcggc gattatattg
    17161 aaattcctcg gctctccgtc ctttgcccct tcaaggtcaa caaggtgcaa gttttccgcc
    17221 cccgcccttt taaactccag ggcggtatca atgggtctgt cgccgaaaac cgtcattttg
    17281 tcatagtccc cttgatatag ccttacaacc ttgccgtctc tgaggtcaat tgcgggtaat
    17341 atttgcattt tatcctcctt aaatctctgc gaaagccttt aaaagtctca gccccgcatt
    17401 gccgcttttt tcggggtgga actgcgctcc gtaaacgagc ccctgccgca caagtcccgt
    17461 aacggtcgtg ccgtattcgg agactgcgag aacgctctcg gcgcagtcct ttgcgtggaa
    17521 gctgtgaaca aaatacaccg actcgccgtt tttaaagtac ttaaaaatcg ggtcgtcctt
    17581 aattattcgg agcctgttcc agcccatgtg cgggattttg agcgaagggt ctatatcgtc
    17641 agagagggga gcgacaatgc cccgaatcag ccccagaccc tcatgctcgc cgaactcaag
    17701 acttttgtcg aataagagct gcattccaag acaaatgcca aggagcggct ttattttagc
    17761 ctgctctttt accgtctcca caagccccgt ttctctgagt ttcatcatag cgtccccaaa
    17821 ggcccccacc ccggggagta taatcttgtc tgccttaatt aaatcctctt ttttgcttgt
    17881 tacaacgcag tcaagaccca agaattttag acttgacttt agcgagaaca aattgcctac
    17941 gccgtaatca acaacagcta tcataaaaga atcccctttg tggacgcctc tccgccgccc
    18001 gtctcacgaa ccgcctcttt caaagccctg ccgagacctt taaacatagc ctcaattata
    18061 tggtggctgt tcttgcccga aagctgcttt atgtgcaggg tgcagccgag gttattggag
    18121 aaagccctca taaactcctc aaccagctcc gtatcaaaaa cccccacctt ctgagaggga
    18181 atctgagcgt caaacgccag ataggggcgg ccgctaacat caagggctat gagcacaaga
    18241 gcctcatcca tgggaagtat tatgctgccg tagcggttta tgccgccgct gagacactcc
    18301 cttatagcct tgccgaggac aatgcccaca tcctccgctg tgtggtgata gtccaccttt
    18361 gtgtcgccct tgcaggcaag gcttaaatta aacccgccgt gaaaggcaaa gagggtcagc
    18421 atgtggtcca taaagccgca gcctgtgttt acttcagcca tgccctcgcc gtcaagattt
    18481 atggtaagcc cgatatcggt ctcattagtg gtccttgtta tggtcttttt cctcatttcg
    18541 ccgcctcctt taaaatatct ttcaaagcct ttaaaaaggc atccatttca tcatcgctgc
    18601 cgacggttat gcgtataaag tcctctattc gctctgtgcc gaagtaccgt acaagaacgc
    18661 ccttttcttt aagcagtctg tagaactcag ctcccgggat tttctcgtgc cttgcgaata
    18721 taaagtttgt catgctgtcg gtcaggaaaa atccgagcgt tttaagctca cccgacaccc
    18781 gctctcttgt ttttataatc tttgaggtgc aggtttcaaa gtacactctg tcttttatcg
    18841 ccgcggcggc ggcaagctgc gttaagcggt ctaaattata ggggtgaaag ctgaatttga
    18901 cagtattaaa atctcttata agctcagcgt cacccgccgc gaaggccgcg cgtccgcccg
    18961 caagactgcg gctttttgaa aaagtctgca caacaataag atttttaagc tcttttacaa
    19021 gccttataca gctttcggcg ccgaagtcca catatgcctc gtcaaccact atgagcctgt
    19081 cgggcttttc ggccgcaagg gcggctattt tctcaggact tacggcaagc cccgtcggag
    19141 cgttgggatt cgcgataact atggtttcgt ccaaatccct gtaatcctca aggcacagag
    19201 taaagtcatc ctttaaagtc acaatcttat aatctatgcc gtaaaactca gcccagacct
    19261 tgtaaaagcc gtaggttata tcagcaaaag ccgcgccctt ggggcaaaag gcttgaaaaa
    19321 tcaaagccag aagctcgtcg ctgccgttgc ccgctattat gttttcgggc gacaaaccga
    19381 agttttcggc tatagcctca tttaacagcc tgttttcagg gtcggggtag agcctgagag
    19441 tctcagcctg tccctccaaa gccttaaaaa cataaggcga cgggggaaag gggctttcgt
    19501 ttgtgttgag cttaatgtat tttccgcctt ggggctgctc acctggggtg tagggtttca
    19561 gccctttaag tcggggggat agaaacttac ccatttccct cacctctaaa gcggctctca
    19621 acgcttcttg cgtgagcctc aagcccttca aagcgggcaa agtgcgcaat cctgtctttg
    19681 acttcgctca gagcctcttt tgtgtagtaa atatagcttg accgcttaat aaagtcgtca
    19741 acgcccaggg ggcttgcgaa cctcgccgtg ccgctggtgg gcagggtgtg gttggtgccc
    19801 gcgaaatagt cgcccaaagc ctcgggagtg taggcgccca agaaaatact gcccgcgttc
    19861 tttatgtccg caaggtattt cttcggctcc tctacgcaaa tctcaaggtg ctcgggagct
    19921 atgtcgttag ctatttcaat tgcctcggag atggaggagc aaaggaatat cgcgctttga
    19981 ttgtcaatgc tctgccttgc tatttcggct cttggtagta attcaagctg cctttcaacc
    20041 tccgcctgca ccttttcggc aagctcctta ttgtcggtta caagaatcgc gctggcaagg
    20101 gtatcgtgct cagcctgact taaaaggtcc gcggcaatat tcacggggtc ggcgttttta
    20161 tcggcaacta tcagaatctc gctgggaccc gcaatcatgt caatgtccac aagcccgtaa
    20221 acgagacgct ttgccgacgc cacatagcgg ttgccgggac cgacaatctt gtctaccttc
    20281 ggaatgctct cagtgccgca ggcaagagcc gcaatcgcct gagcgccgcc cactttaaag
    20341 attttatcaa tgccggcgac cttagcggca gccagcacag cgggatttac cctgccctct
    20401 ttgtcggggg gagttacagc gacaatctcc gaaactcccg cgattttggc cggaatggcg
    20461 ttcatgagca ctgttgaagg atagctcgcc gtaccgcccg gtatgtaaag acccgctttt
    20521 tcaatgggtc ttacaatctg accgagctca gagccaaagg gagcggtcat actgaaaccg
    20581 tcgcttagct gctttttgtg aaatgccctt atattctccg ccgccatttt tagagtatcg
    20641 aggaagtatt ctcccaccga ctcagccgcg gcgtcgattt cctcttgagt tacaagaaga
    20701 ctctccggtt tgcagccgtc aaagcgaagg gtgtactcga aaagagcctc gtcgcccctt
    20761 tccgccacat cgtttattat ataccgcaca tcagcgtcag cctgcgctct ctcacgctta
    20821 aaagctttgc tgtcatactc cgcaatcttt atcatttaat cacctcggtc atcttttcgc
    20881 aaatcctgtc gatattcgag ctctggaact gatatgtcac cttgttcgct ataaggcggg
    20941 cgcttatcgg cataatctcc gccagtacat caaggttgtt ctctttcagc gtcttgccag
    21001 tttcaactat gtccactata acatcggaaa gacctaaaag gggagccagc tcaatgctgc
    21061 cgccaagctt tataatgtca acctctctgc caagggactc atagtattgt tccgctatgt
    21121 tcacaaactt tgtggcgacc ctgagtgtcc ttgagccgtc gtccacaaaa tctttgggac
    21181 cggcgacgca cattctgcac ttgccgatac caaggtccaa cagctcgtag acatccgctc
    21241 tgtcctcctc aagaatgtcc ttgcccacaa ttcccaagtc cgcggctccg tgctcaacat
    21301 aaatcgctac atcggagggt ttaacaagga agtacctcag ccccgcctct ttattctcga
    21361 taaccagttt tctgcccata ttgtttatat cgccctcggc aaagccggcg tttttcatga
    21421 tatcgtaaac atcatcgccc agcctgccct ttggcagtgc aatgctaagc atgacgctca
    21481 cctccgaaca gttcgctaag atacagggca aagcctattg cgcgcccctc tttgttcatc
    21541 tttttcagca tattgtcata ctgaccgccc gacagaacca cgcggctcct gccctttata
    21601 tatccgcaga acataagccc gctgtagtaa tcctcatcgc ccctgatgga gaaatcaatg
    21661 agaatgttgg agctgtcaag cccgtcaatg gcgtctttaa tctgtcccac cgcccgatgc
    21721 atttcggaat ttttgcagta tttgaaggcg gtgtcaatga ccttgtcagc cccgccgctg
    21781 aggagggcgg ctttttgcag aaaatctatg ttctcgcccg acagtcccgc ggcctcagcg
    21841 gtcattctaa gcccgagggg gttttttgag gcaatactct catacaaggc gccgcggtac
    21901 tgggtcggaa ctttaaggct cttcattagc cccgcaataa agcccacatg gctgacggag
    21961 agcctgaacg gctttcctgt gagtttaagg ctttcgtagc tcagatacaa aacctcggaa
    22021 acctcagcct tgccgacttt gccaatgcac tcaagaccca tctggctaat ctccgcaaag
    22081 ctcctgcctg tcggacccgg gcgatagaca ttttctatgt aataaagctt attaagcccg
    22141 ccgtttgagc cgtagtgttt gacaatgcta agcgtcacat cgggctttaa agctctcagc
    22201 ctgccgtcaa ggtcgctgaa ggtgattagc tgctcgctgc ggagaaagtt tatattctca
    22261 aggtagaacc tatactcttc aaagcggttt gccttaatgc gccgatagcc cttgctttca
    22321 aacagagccc tgaatgaaag aacggtgcgc tcctgaggcg acagaatatc gagtaaattc
    22381 tccattttga tgcctctctt tactatgata atatgatagt atgatattat agtagcagat
    22441 tgaaacaatt gtaacacgaa cgggaataaa atgctatcca aaaatttgcc ataaatatat
    22501 gttcactctg ccgaatttct tgtcacaata aacaagagct gcccaaaagc cttaaatatt
    22561 aattgcattt ttgtaatttc gaaatttttt gtgataatct aaaaaaaaac aaaacatttc
    22621 ctcattctca atcgtattag gagtgaaagg gggagaaatt ttgcagagtt ctttattgcg
    22681 tacgaatgaa gagctgactg agatttacag ccgccatgtt gacactgttt acagagtctg
    22741 cttcatgttc atgaaaaatg ggcccgatac agaggatatg gtccagaaca cattcataag
    22801 gctcatgcgg gacaataccg tctttcaaag cattgagcat gagaaggcgt ggcttataag
    22861 gaccgccacg aatctttgca aggaccactt aaagcactgg tggcaaaaaa gaagagccgg
    22921 tatggacgag gcggaaaaac tcgagagcga acccgccttt gaggcagaca ataatctgga
    22981 aatggtgctg aatctccccc ctaaatacaa aacggccgtt tatctctatt actacgaggg
    23041 ctacaaaacc gccgagattg ccaaaatgct cggcaaaaac gaatccacca taagaggctg
    23101 gctatataaa agcagacagc ttcttaagct ggaaatggaa ggtgatttac agtgaggaaa
    23161 gaggatttaa aggcttccct tgataaaata aagcccgacg aggcggcaaa aagaagaatg
    23221 aggtacaata ttatgaatta caacgaagaa aagacagcca gaccgatttt gtggaaaaga
    23281 gccgttcccg cgctcgctct tgtgctggtt ctcaccgcgg gcatattcgc ctacgataag
    23341 attttcagcg aggccaatac ctacaagccc cccgtgctct catccgaagc cgacgattac
    23401 gcgcgggagg acatggcagc tcccattgtc aatcagttta agttcgaaga cagggactat
    23461 ataattttgt ccgatgagct gagaaaggaa ttcggtctgc cttctgaaat ccgagacggc
    23521 gatataggcg ataagcttgg aaccattact cagggtcccg acgaaaagct cattggcaaa
    23581 gaggtttacg agtatatccc cgcggactgc gaggcgattg tagcggtcaa ggatggcgac
    23641 tcctacaagc ttttccgatt ctttaacttt gaaagctaca aaaataacat cgacgaggac
    23701 gccgaaagat acttaaagct tttcggaatc aagagcgcag aggatatctc aaaggtccag
    23761 ttcatccttt actccgagga ggcaaagcta agaggcggct ttgacatcaa agctgagctt
    23821 aagggcaagg acgaaatcaa aaagttctat gatttctatt caggtttaaa gaactcctcc
    23881 gacgagtact tcaaggcgct gtataactac cgccgggaag gcattgacca cggtaaagaa
    23941 gtttatcccc ccgtgcccga caccgccgtt agccaaatag ctcccgacgc ccccaacgcg
    24001 tcttcccccg tattccccga cggagcagag cccgcatact atgccgatga tttgcccgcc
    24061 gctgttcccg aaagagcaaa cggcgcggcc accccggcga atgacggaaa tctcagtact
    24121 gtggtaggtc acagaggaat gatggacatc ggcgaaacag gcgttgagac tgtcaccgcg
    24181 aatcagggct cagcgggagc cgcgctggag aacgctgtca caatcagaat ctacaacaaa
    24241 aacggcatct actatgacac gatttactat cccaatatcg gctttatcag caggtataag
    24301 ctcggcgaca atttcaaagc cttcttgaac tcctatgtaa aatagcctgc ggttaaacac
    24361 tgaccgagaa cttatgttct cggtcttttt tcttttattg cgaaaaagcc ttaagaaaaa
    24421 agtcgaaagg cgttatgatg aaaatagaaa aaattggcag taggtgagat tatgcccgag
    24481 aaggtaaatc tgaaagaagc ggtgaacagc ctgacagagc ttttcgagta caaaaaggtc
    24541 ggctacttta acaatcagat gatgaatgtg cttattgcgg agaaccgcac ccttgattat
    24601 catgtgcatg aaaactccga ggaggttttt tactgcatag aaggggagtt tgacattgaa
    24661 tttgacgacg gtcccgttca ccttgccgaa ggagatttca ttattgttcc aaggggcacc
    24721 cgccaccgcc ccgtatgcaa gggtcttgta aaatgccttc ttattgaaaa cgagggcacc
    24781 ctcaacgccg aaaacacagg cggcgcatat aaaccttaaa gagttttgat tcgacattgt
    24841 tttgttgtaa aaacaacata ccttttttcc tcccaatggt ataatcggca gcgttaagac
    24901 aaaaactgcc gtttttatag cgggcacatt atgaaaggag aaggacaaat gaaaaagtac
    24961 atttgcattc cctgcgggta tatttacgac cccgaagtag gcgacataga cggcggcata
    25021 gcccccggca cagcctttga ggacattccg gacgactggc agtgccccat ttgctttgta
    25081 ggcaaagacg agttcgaagt ttttgaagat taagaggagg aaaagaaatg agtatgttct
    25141 gttttcaatg tcaggaaacc gcaggcggaa agggctgcac cgtcagaggc gtttgcggca
    25201 aaaccgagga ggtcgcaaag cttcaggacc ttctgattta cacccttaaa ggactttccg
    25261 aggttgttgt gaagggcgga tttaatgcaa aagatttagg cggcataaac tatcagcttt
    25321 taaacagcct ttttatgacc atcaccaacg cgaactttga ccccgaggca atagaaaagc
    25381 agattgtaaa aatgctctct gtccgcgacg aattaagaaa gaagtccgac aaagccgatt
    25441 tc
//
LOCUS       DAONVQ010000009        24428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_1416_length_24428_cov_4.858327, whole genome shotgun sequence.
ACCESSION   DAONVQ010000009 DAONVQ010000000
VERSION     DAONVQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 24428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24428
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1416_length_24428_cov_4.858327"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLD98_01400"
     CDS             <1..598
                     /locus_tag="PLD98_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HQA04941.1"
                     /translation="KRIIFNGNGYDESWIKEATEKRGLSNYRTTPDCMPHLLDKKNVD
                     MLIKHGVYSLAELKSRCEIMLENYCKTVIIEANTMVDMAKTQIAPAVELFTADIARGA
                     AAKKALDASLECSYEKNVVIKLSALLDRIAAKTDELSSALVSVGNAEDIETKAYRIRD
                     EILPLMGELRLACDEAETLTAKSYWPFPTYADLLFGVK"
     gene            750..2366
                     /locus_tag="PLD98_01405"
     CDS             750..2366
                     /locus_tag="PLD98_01405"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQA04942.1"
                     /translation="MTKYIFVTGGVVSGLGKGITAASLGRLLKARGLKVAAQKLDPYI
                     NVDPGTMSPYQHGEVYVTEDGAETDLDLGHYERFIDEDLNKYSNLTTGKVYWNVLNKE
                     RRGEYLGSTVQVIPHITNEIKEFIYRVGRKTNADVVITEIGGTIGDIESQPFIEAVRQ
                     ISLEVGKENSLFIHVTLVPYLRGSGEHKSKPTQHSVRELRSLGLNPDIVVLRCDEPLE
                     DSIFKKISLFCNVKPDCVIENITLPNLYEVPLMLEKANFSKVVCRELNIDAPEPDLKE
                     WTEMVERLKSRPYTVNIGLVGKYIGLHDAYLSVTEALHHASIYHNTHVKIHWIDSEEI
                     TSETAEAKLSGLDGIIIPGGFGSRGIEGMIQAARYAREKHIPFFGICLGMQVALIEYA
                     RNVAGIEDANSGEFDEQCKHKVIDFMPGQSDHIDKGGTLRLGSYPCVIKPGTTMERCY
                     GTTLINERHRHRYEFNNEYRELLKEHGLTLSGMSPDDRLVETVELSDRPFHVGVQYHP
                     EFKSRPNRPHPLFKGFIEAALKQSGIKKED"
     gene            2394..3185
                     /gene="dapF"
                     /locus_tag="PLD98_01410"
     CDS             2394..3185
                     /gene="dapF"
                     /locus_tag="PLD98_01410"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQA04943.1"
                     /translation="MRFTKMHGLGNDYLYIYDEVPQNVSELAVKLSDRHFGAGSDGLI
                     YISKSNVADFKMRIFNADGSEAKMCGNGIRCVGKYVYDKGYTDKTTLRIETLSGIRTL
                     NLQIVGSKVKSVTVDMGKAAAEKPITLDVDGKSVTLTPVNVGNPHAVIFVDDIEKAPL
                     YELGPKIEKHKHFPDGVNVEFVQVQSPLKLRMRVLERGSGVTMACGTGACASAMAAIA
                     SKYCKYGDEISVRLDGGDLKIQISPDNSVQMTGPAEFVYEGETIE"
     gene            3182..4366
                     /locus_tag="PLD98_01415"
     CDS             3182..4366
                     /locus_tag="PLD98_01415"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HQA04944.1"
                     /translation="MTTPNMNYANLKDSYLFFNIGQKVRRYLEQNPDKRVLRLGIGDI
                     TRPLCDVVIKALHEAVDDQASFDRFHGYMPEVGAPFLREAIRNHYADRGIELKVDEVF
                     VSSGASDELGDILDLFDRSNSALVIEPAYPAYVDANVIAGREIIHLASSKENGFLPHP
                     TDDAKADIIYICSPNNPTGAVFSRSQLKEWVDFANKHGSVILFDAAYEAFIEDDSLPR
                     SVFEIEGAKTCAIEICSLSKTAGFTGTRLGYTVIPKALVRNGMSFNDMWVRNRTTKTN
                     GVSYILQKGGAAVFTPEGQQQIKENISVYKNNAKLIMNALDEIGLWFCGGKNSPYIWL
                     ECPKGMGSWEFFDYLLNEIQVVGTPGQGFGECGEGYFRFSTFGSPEDTKEAARRLCEL
                     LR"
     gene            complement(4564..5727)
                     /locus_tag="PLD98_01420"
     CDS             complement(4564..5727)
                     /locus_tag="PLD98_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HQA04945.1"
                     /translation="MYKVLTLNKISAEGLSNFDSAKYSCSTEFDSPDAIIVRSASMHE
                     MELPESVLAVARAGAGVNNIPVSAYTEKGVVVFNTPGANANAVKELVICGLLLSSRRI
                     VPAIEWCQSLKGKGDEVSALVEKGKSNYTGPEIKGKTLGVIGLGAIGVMVANAAVALG
                     MKVVGYDPYLTDKAATSLDKSVKITANLDDVFAESHYISLHAPLTDSTRGIVRAETLE
                     KMRDGVRILNFARKELVAENDIIEALNSGKCAAYVTDFPSDSQLGVDGVIPIPHLGAS
                     TPESEENCAVMAVEELKAFLEKGEIINSVNFPNIELAPSEDAKERLLVLSAEDVSEKI
                     LGILANNSIKVAGGASNAKKAAAALIETADAIPENVLDEIKAIEGVYRVRVIK"
     gene            complement(5783..6865)
                     /gene="serC"
                     /locus_tag="PLD98_01425"
     CDS             complement(5783..6865)
                     /gene="serC"
                     /locus_tag="PLD98_01425"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HQA04946.1"
                     /translation="MPRVYNFSAGPSMLPVEVLQRAADEMLDYKGSGQSVMEMSHRSK
                     VFESIIKEAEELVRELLNVPDNYKVLFLQGGASTQFAAIPLNFMNGSGKADFIITGQW
                     ANKAYQEAAKYGQARIVASSKDKTFSYIPKTKREDFDKEADYVHICLNNTIYGTKYHE
                     IPDVGDIPLIADISSCIMSEPLDVTKFAMLYAGAQKNIGPAGLTLCIIREDMLGKARD
                     ITPTMLNYTIMAENDSLYNTPPCYSIYICKLVLEHLKATGGLEAARERNIKKAKLLYD
                     YLDSSSLFKATVVPEDRSLMNVPFVTGSEELDAKFVKEATAAGLVNLKGHRTVGGMRA
                     SIYNAMPYEGVQALVDFMKKFEAENK"
     gene            complement(7036..8208)
                     /locus_tag="PLD98_01430"
     CDS             complement(7036..8208)
                     /locus_tag="PLD98_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HQA04947.1"
                     /translation="MDYLKESLKLHEKLRGKIEIVSRAPVKSREDLSLAYTPGVAAAC
                     LEIKEHPEKSFDLTRRWNTVAVVTDGTAVLGLGDIGPEAGMPVMEGKCVLFKEFGGVD
                     AIPLCIRSKDVDEIVKTIALLSGSFGGINLEDIAAPRCFEIERKLKECTDIPIFHDDQ
                     HGTAIVTVAALLNALKLTDKKLEDLTVVFSGAGAAGVAIAKLFKSLGIENIIMCGRKG
                     IISRGDDSLSDAHKDLASWTNPQNLKGTLADAIKGADVFIGVSAPNVLTPEMVKTMAK
                     DAIVFAMANPVPEIMPDLAKEAGALVVGTGRSDFPNQINNVLAFPGVFRGAFDVKARD
                     INEEMKLAAAMAIASLVSDEELSHDYIIPKAFDERVAPAVAKAVSKAAIETGVARI"
     gene            complement(8237..9754)
                     /gene="glpK"
                     /locus_tag="PLD98_01435"
     CDS             complement(8237..9754)
                     /gene="glpK"
                     /locus_tag="PLD98_01435"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HQA04948.1"
                     /translation="MGKYVLALDQGTTSSRTIVFNSQGEIVSMAQYEFPQIYPQPSWV
                     EHDPYEILKSQFRSLNDAMISGNIKSTHIAAIGITNQRETTVLWDKETGKPVYNAIVW
                     QCRRTAAMCEQLKAEGLEDYIKEHTGLLLDAYFSATKIKWIIDNVPLAKQLIKEKRLL
                     AGTIDTWLIWNMTKGAVHVTDYSNAARTMLFDIDRLCWDEYLCEKLGIPMEILPKVMP
                     SSAVYGTVAEGVPGIEALAGVPISSAIGDQQASLFGQACYEAGDAKNTYGTGCFLLMN
                     TGEKRIRSKHRLISGIAWGYDGKVNYCLEGSAFNAGSVIKWLRDELKLIKNAKECDTL
                     AETVPDSNGIYLVPAFTGLGAPYWDMYARGCIVGLTRGVNRAHVCRAVLESIVFQLKD
                     LLEAMNADTGISVNELRVDGGASVSDIMMQIQANIANIRVNRPKVVETTALGAAYLAG
                     LAVGFWKDMDEIRDIRKSDRIFEPSMDDREREKLYAGWKRAVERSMRWAAPEIDE"
     gene            complement(9910..14865)
                     /locus_tag="PLD98_01440"
     CDS             complement(9910..14865)
                     /locus_tag="PLD98_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04949.1"
                     /translation="MLTVVLILSCFSAGFVGFAASASQLAALAEAIKAKPNVSTLSSY
                     SVSSNSVTVSSDTTNGIWDAAYKFYQAALSTAVLNTAGDRNDNTDNNTGPEVIATLRN
                     QLNSPDYFTSEEYAQYNVNTLMQYFAGNPYNIREVGSTGSLNAQNCTFTVNRTLKAAL
                     LSYASVDDVPNTINLRATYTWVHSVASASGGLFQTYRWHYLTAINYAESQPQNNVKAD
                     LDAYRSVFTPETLNIDLSTLTDEEIVDLYDAAVAALERGNRFDNEIMEHFGLPTLTAA
                     AAFVANLKYYADISRYRPDVLYFNNVLALDYSEFTLEQKRELRTASTAAFNRLNVLRT
                     DSALQAVRQFFEAHYDLDFNAIDTFRRELNHDIECIELQQYKDYFDDVIANLVIPFEE
                     EGSLSNTEFYAFVNEFTAKYNAVVGTNASGVPYYTSEAKVAIFTEGTDYVGEYLALLN
                     EELVARNLLDESGQYYEYFNESRLASDASLMDKATIIAEFNTGCQKLQQINAYDLEIR
                     IRIFGEYYDRIVAYIDSLGYEIADRLEAQLNIAAERYYDYGGIKLINYKEVRELIGMV
                     EREYYPLIQNTPYNNSSIQSKYNWLDPALAEYNDFVAKKGLVGPNGTGGHYKLEPELP
                     VRVVREDDIIRIISPDHDYVVTKDRLLNVIDKLDEFLNSQEFGKLIDYGIVEETTVTT
                     TGTTAPTTTQPGTTEPVETEPPVPPINVSLPEINQARLASLGTLISFSSTRSSGVGHR
                     IIADRADVLVAVLNYLLNALGDETFLSTVVSALEGLFDGDDDEEEPAEPEEPGEPEEP
                     GEPEEPGNVIYMVFKDILNDLINGLFTQDSVNSIVGTLYPELANTVNEQLGGTMGTIA
                     SLFGITPAAMGLNVFPEDLADQINTSKYPAVRTALKAAGKDWSKVMNEDGDVVLDWGF
                     PEGDRDEWIQAMASAFAGVTALLKAAWGNREHTFSRSILLAGINLNVTRNRGYDKAIM
                     PFLEAIGAPGVVSTATFEGYCNNTTINYTVNLNMLRAIFNPLFDWIQVNMANNPLKTI
                     LDFLPNMAYAMEFSMFDQGFADLFDTVITIKGSGLASGVNETIYVSDMINPDDLMGGL
                     GIELTSLNDMLGSLINMLANKDRNTDGWVPPVDENGNPIPVDPGAVEIDGTLKEILQG
                     LAENPGDAIAAIVELFNPVKYPNLTLTYPHAELAGLTPPENQIVKVGYSSYWTTERAD
                     FVADNLNEFVDSLLKLLGLGSIQELLDKLFGESVYTKKNMTKLAEMLSELLSDDDIGD
                     ILDIVAPLLGLDLDSFKNPKLALDFEDGDKDGFVAALVEFLAPVEPLLRVLLVGDDLP
                     LLSLTTIYGYEGYRTGIIPILEALGIEESEILDFETYKAQADEDGANLLLNIINPILS
                     LIDRIYEAPVETLYEILPNIFYFINADLLNVSLINLLQPVCVLLDTVRPIYNVNLGLD
                     VSISEMLADLVAGLDIAGLRVPALPELETFAAGTVTTYQSKAGDAVVGDAVYIEGYSR
                     DFLTILLRFLVQTVFFRDNTEILKAALADATGLDGAGLATVNTMIDALSAYARRPEGT
                     DLVLMVLFYLLSGGNKAIGQMNDFLGGFNAEVKYIFDLFANSDSEFLRDFAEAAAELL
                     RQISGGIFDDGGLASSGLIRFFQRIIEFFKNLLKIFSFIGA"
     gene            complement(15251..16549)
                     /gene="eno"
                     /locus_tag="PLD98_01445"
     CDS             complement(15251..16549)
                     /gene="eno"
                     /locus_tag="PLD98_01445"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQA04950.1"
                     /translation="MAGTSIQRIRAREIIDSRGNPTVEADVILENGVIGTAAVPSGAS
                     TGAFEAVELRDGDKGRFGGKGVSKAVDNVNGIINDELKGMCVFNQADIDLTMIQLDGT
                     ENKGRLGANAILAVSLAAAKAAANTLGIPLYRYIGGCDARTLPVPMMNILNGGAHASN
                     NVDIQEFMIMPIGADSFAEALRRCCEVFHSLKKVLDDQGLATGVGDEGGFAPNLGSEE
                     EALDAILEAIKKAGYMPGTEFKIAIDAASTEWYRDDGTYFMPKKKKKLTRDELISYWE
                     SLIDKYPIISLEDPLGEEDWEGWQAITEKLGSRLQLVGDDLFVTNTKRLKKGIELNTA
                     NSILVKLNQIGSLTETIDAVKTAQRANYTAVISHRSGETEDTTIADLAVALSAGQIKT
                     GAPSRSERVAKYNRLLRIEEEIGRPAVYPGRLAFYNIDLD"
     gene            complement(16827..17426)
                     /locus_tag="PLD98_01450"
     CDS             complement(16827..17426)
                     /locus_tag="PLD98_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQA04951.1"
                     /translation="MSSEFGSRLTSLRHERGVSQKEVAEALGVSQALLSHYEKGIREC
                     GLDFIRKASNYYDVTADYLLGLSENRRGLGDLFESSELPSDEDMKMQTIYRAIIKLGE
                     IMTAKGGKKAESVKWYLAFSAYHLLSKAREAGTVPDDWFSLSEDAGTNISNAMANYFS
                     DSFKKEELSEESESVPDILVFKTLISNCEKQIKSILSEI"
     gene            complement(17454..17918)
                     /locus_tag="PLD98_01455"
     CDS             complement(17454..17918)
                     /locus_tag="PLD98_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HQA04952.1"
                     /translation="MSIEKVRAFFKKHGCEDRVLEFDVSSATVELAAKALGCPPERIA
                     KSLTFKSDDGVIMVVAAGDARVDNKKYKAQFGIKAKMLSAEEVEQLVGYPVGGVCPFD
                     VNDGVNVYLDQSLKRFETVYPACGSSNSAIELNLTELEFYSNAKGWVDVCKL"
     gene            complement(17943..19850)
                     /locus_tag="PLD98_01460"
     CDS             complement(17943..19850)
                     /locus_tag="PLD98_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQA04953.1"
                     /translation="MALVSLQNLEMSFLDRLLFTASNINVYENDRIGFIGENGSGKTT
                     LFRLLCGELEPTSGQVIISKSTKIGYAEQHACRDLSKTAYEEMLTVFSHLSEMEKELN
                     ELHSKVDAAKGEELSQLIAKQDALTERFQREGGLTYKNMARSALLGLGFSEEEINSPV
                     GNLSGGQRTKISLGKLLLSDSDLILLDEPTNHLDINSVEWLEGFLQNFKGAFIVISHD
                     RYFLDRVTDKTLEIYGKKLYLGKGSFTRYMELKEERLLSEQREYEKTLKEIKRLEDII
                     EQQRRFNRERNYIVIASREKQIERLKENLGDPPVPPSKAMRLKFEERIESPNEVLTIR
                     EISKSYGEKRLFSNLSLTVKKGERLMIVGPNGCGKSTLIKIIAKHLTPDTGAVFYGPN
                     IVVGYFDQTLSGLNDENSCIEEVYNSCLDKSIPELRNYLAAFNFKGDDIEKKVGSLSG
                     GEKAKLALLKLMLKRPNLLILDEPTNHLDIMSREALEQALSDYSGTIIAVSHDRYFIN
                     RLATNLLAFTADVVKEIDGNYDSYLSFQQGTAPESSKPEKKVNEYKRRKEEESLRRKR
                     QTRLKKIEAELQEVSKELEETNTLLQSPEVASDYERLTELTAKLSALSEKEEELLAEY
                     LQLEEILSEQN"
     gene            complement(19989..20981)
                     /locus_tag="PLD98_01465"
     CDS             complement(19989..20981)
                     /locus_tag="PLD98_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HQA04954.1"
                     /translation="MKKIKSLIALALVFAMLAAYAPFASAVKLKPDLAGKPNTGDVDN
                     KGDVTAADARLVLRYCAKLESFNSDQLKAADVDMDGHVTPLDARAILRIAAGLDMSVN
                     IDLTYRSEADQAIKDAIKAYNTAATKIKKNEVQKNAKVEQTINTKADISLSWALKLFL
                     RSLGQDPSELEQGFTYPADQSTYYLDSNGLVKALPPEGSNAVDIPLDAQTIYGMLKTN
                     QQCTLFFNPENFSNDSVYTKALPVKDFAKLNDPFDLMDDAGDDLGMLIKIKSKSLKYN
                     GGSLFYELDSEGNLTKATYYCQAVATYKMEFLETSTFTMDMKITIDITQEFIFS"
     gene            21349..21777
                     /locus_tag="PLD98_01470"
     CDS             21349..21777
                     /locus_tag="PLD98_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HQA04955.1"
                     /translation="MNRVAVVYWSGTGNTEKMAELVAEGVVAAGGRADIFTAAEFTKE
                     MVQNYKALAFGCPSMGVEELEDTEFEPMFSSVKEFLKGKVIGLFGSYDWGDGEWMRNR
                     VADCEDYGLTLINDGVIANNEPDDEAAENCRALGAALAKA"
     gene            21901..22632
                     /locus_tag="PLD98_01475"
     CDS             21901..22632
                     /locus_tag="PLD98_01475"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQA04956.1"
                     /translation="MEVSDIMRARTSRRSYTSSPIPKDSIEKLQDTIEYLNRKSGLHI
                     QLVLDGEAAFKSFKKTYGMFSGVKHYFAMVGNSSDPLLHEKVGYYGEYLVLVATELGL
                     GTCWVGSSYDAQNCNCEILAGEQLVCVITVGKSPESLSSRERLVLSMVKEKSKKYDEL
                     CQYDKQPPEWFWDGMKAVELAPSARNRQPVQIIYYNDVIYATTGQFQYNKIDMGIAKL
                     HFALGAGGRFELGEHGRFYKDEETV"
     gene            complement(22673..23563)
                     /locus_tag="PLD98_01480"
     CDS             complement(22673..23563)
                     /locus_tag="PLD98_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HQA04957.1"
                     /translation="MAEVVGVRFKVVGKIYYFDPAGVQLKKGDNVIVETARGIECGEI
                     AMENRDVDEDQIVKPLKSIIRKATPEDLKIVEENKEKEKRAFKICEERIQAHGLEMKL
                     IDVEYTFDSSKILFFYTAPTRVDFRELVKDLASIFRARIELRQIGVRDEAKMLGGLGI
                     CGKPFCCSQFLSDFEPVSIKMAKEQGLSLNPVKISGSCGRLMCCLKYEQEVYEHLARH
                     TPKVGAIVETADGRGEVVESNLLTGVLKVILDSKRDAAPQTYHCKDVKLIMNPKVKIE
                     KEELEALKEIAEEPVDETLS"
     gene            complement(23582..>24428)
                     /locus_tag="PLD98_01485"
     CDS             complement(23582..>24428)
                     /locus_tag="PLD98_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04958.1"
                     /translation="SGRLSHAVLITGATEKIRAQVARLTASALLCVGDRDKPCGKCGH
                     CVKSLKNLHPDITEISGEDKAKSIKKESVLLIRQQALIMPNEASEKIFIVFEAQNMTE
                     EAQNAMLKILEEPPRFARFILTAASSEGLLETIRSRVRTFDLGEESQESKQNKAQTIA
                     CDIARALYSGSEYDLVLAAAPIKKDRQLIKNVCDELTGIFIEALKLQKRGFAKTEGYP
                     ENESQAMAVKMYKSVSPSLLLRWTGTLSKIAELAEMNANETLLSTFFCSRLSEDKIGM
                     TANEN"
BASE COUNT         5979 a         5741 c         6031 g         6677 t
ORIGIN      
        1 caagagaatt atcttcaacg gcaacggcta tgacgagagt tggattaaag aggcaacaga
       61 aaagaggggt ctctccaact accgcacaac tcccgactgc atgcctcacc tgcttgacaa
      121 gaagaatgtt gacatgctta ttaaacacgg cgtttacagc ctggcagagc ttaaatcaag
      181 atgcgagatt atgctcgaga actactgcaa gactgtaatc attgaagcta acaccatggt
      241 tgacatggca aagactcaga ttgctcccgc cgttgagctg ttcaccgctg acatcgcaag
      301 gggcgcggcc gctaagaagg ctctcgacgc atcccttgaa tgcagctacg aaaagaatgt
      361 tgtaataaaa ctctccgctc tgcttgacag gattgcggca aagacagatg agctgagcag
      421 cgctctcgtt tcggttggca acgcggaaga cattgaaaca aaggcatata gaattcgtga
      481 cgagattctg ccgctcatgg gtgagctgag acttgcctgc gatgaggccg agaccttgac
      541 agctaagagc tactggccgt tcccgaccta tgcggatctg ctctttggcg taaagtaaat
      601 gggctttacg ccaaagactc tactgtaaag caatgacact tgacacaatt cttattcaat
      661 aaagcaataa ggctttacat ttaacagtgt aaaaaagttg gatttggtca aaaattattt
      721 tagattcagt catcagcgga ggaaatatta tgacaaaata catttttgta acgggtggag
      781 ttgtatccgg ccttggcaaa ggcattaccg ccgcatccct gggaaggctc ttaaaggcaa
      841 gggggctaaa ggtagcggct cagaaactgg acccttacat caatgttgac cccggcacca
      901 tgagcccgta tcagcacggc gaggtatatg ttaccgaaga cggagctgaa accgacctgg
      961 acctgggaca ttatgagcgg tttattgatg aggatttgaa taaatactca aatctcacca
     1021 caggcaaggt ttactggaat gttcttaata aagagcggcg gggcgaatat ctcggctcca
     1081 ccgttcaggt gattccccac atcacgaacg agattaagga atttatctac agagtaggca
     1141 gaaagactaa tgccgatgtg gtcataacag aaatcggcgg caccatagga gatattgagt
     1201 cccagccctt tattgaggct gttcgtcaaa tctcccttga ggtgggaaag gaaaacagtc
     1261 tttttatcca tgtaacgctc gtgccgtatc ttcgcggctc cggggagcac aaatccaagc
     1321 ccacccagca ctctgtcagg gaactcagga gccttggtct caaccccgac attgtggtcc
     1381 tccgctgtga cgagccattg gaggattcaa tcttcaagaa aatatccctc ttttgcaatg
     1441 taaagcccga ctgcgttata gaaaacatca cactgccgaa cctttacgag gttccgctga
     1501 tgctcgaaaa ggcaaacttc tcaaaggttg tttgccgcga gctgaacata gatgctcccg
     1561 agcccgacct taaagagtgg accgagatgg tcgaaaggct gaaatcaaga ccctacacag
     1621 tcaacatcgg acttgtgggc aagtatatcg gtcttcacga cgcttatctc tcggtaacgg
     1681 aggctctgca ccatgcgagc atctatcaca atacccatgt aaagattcat tggattgact
     1741 ccgaagaaat aacctcagaa actgccgaag caaagctctc cgggcttgac ggaataatca
     1801 ttcccggagg ctttggcagc aggggcattg agggcatgat tcaggctgca aggtacgcca
     1861 gagaaaagca tatacctttc ttcggaatct gccttggaat gcaggtggcg ttaatagaat
     1921 acgcaaggaa tgtggcgggc attgaggacg cgaattccgg tgagtttgac gagcagtgca
     1981 agcacaaggt tatagacttt atgcccggtc agagcgacca tatcgacaag ggcggcactc
     2041 tcagactcgg ctcatatccc tgcgttatca agcccggaac aacaatggaa cgctgctacg
     2101 gcacaactct tataaacgag cgccacaggc acagatacga gtttaacaac gaataccgcg
     2161 agctattaaa agagcatgga cttacactca gcggcatgtc gcccgacgac aggctggttg
     2221 aaacggtgga gctgagcgac cgacccttcc atgtgggcgt tcagtatcac cccgagttta
     2281 aaagccgacc caacagacct cacccgctgt ttaagggatt tatcgaggct gcgctgaagc
     2341 agtcgggaat taaaaaagaa gattagtatc tcacagcgta aaggaggggc gatatgcgtt
     2401 ttaccaaaat gcacggcttg ggaaatgact atctgtatat ttacgatgaa gtgccgcaga
     2461 atgtgtcgga gctggctgta aagctttccg accgacattt cggcgcgggc tcggatggtc
     2521 ttatatacat ttcaaaatca aatgtcgcgg actttaagat gcgcattttc aatgccgacg
     2581 gcagcgaggc gaaaatgtgc ggaaacggaa ttcgctgcgt tggaaaatat gtctatgaca
     2641 agggctacac cgacaaaacg accctgagga ttgagaccct atctggcata cgcactttga
     2701 atcttcagat tgttggcagc aaggttaaat ccgtaacggt ggatatgggc aaggctgcag
     2761 ccgaaaagcc cataactctc gatgtggacg gaaaatccgt aacccttacc cccgttaatg
     2821 tgggcaatcc ccacgcggtg atttttgtag atgacattga aaaagctccg ctttatgagc
     2881 ttggacccaa aattgaaaag cacaagcact tccccgacgg agttaatgtg gagtttgtgc
     2941 aggttcaaag cccgctcaaa cttagaatga gggtcttgga aagaggcagc ggcgttacaa
     3001 tggcatgcgg cacgggcgca tgcgccagcg cgatggctgc catagcctca aaatactgca
     3061 aatacggtga tgaaatatcg gttcgccttg acggcggcga cctgaaaata caaatctccc
     3121 ccgacaacag cgttcagatg accggtcccg ctgaatttgt ttacgaagga gagacaatcg
     3181 aatgactaca ccaaatatga attacgcaaa tttaaaggat tcgtaccttt ttttcaatat
     3241 aggtcaaaag gtaagaaggt atcttgaaca gaacccagac aaaagggtac tgcgtcttgg
     3301 catcggcgac attacccgcc ccctttgcga tgttgttatc aaagccctac atgaggctgt
     3361 agatgaccag gcgtcttttg acaggttcca cggctatatg cccgaagtcg gcgctccttt
     3421 tctgagggaa gccataagaa atcattacgc agacagaggc attgagctta aagtcgacga
     3481 ggtttttgtt tcaagcggcg caagcgatga gttgggcgac attcttgacc tttttgaccg
     3541 ctcaaattcc gcgcttgtaa tcgagcccgc ctaccctgct tatgttgacg cgaatgtaat
     3601 tgcgggaaga gaaattattc atcttgcgtc aagcaaggaa aacggctttt tgccccatcc
     3661 cacagacgac gcaaaagcgg acattatcta catatgttcg cccaataacc ccacgggcgc
     3721 ggttttcagc cgcagtcagc tcaaagagtg ggttgacttc gcgaataagc acggctcagt
     3781 aatcctcttt gacgcggctt atgaggcgtt tattgaggac gatagtctgc cgaggagcgt
     3841 ttttgagatt gagggcgcaa agacctgcgc cattgagatt tgctccctgt caaaaacagc
     3901 gggatttacg ggaacaagac tcggatatac cgttatccca aaggctcttg tgcgcaatgg
     3961 catgagcttt aacgacatgt gggtgcgcaa ccgcaccaca aagaccaacg gcgtatccta
     4021 tatcctgcaa aagggcggag ccgctgtctt tactcccgag ggacaacagc agattaaaga
     4081 gaacataagc gtgtataaaa acaacgcaaa gcttataatg aacgcacttg acgaaatcgg
     4141 actttggttc tgcggcggaa agaactcccc gtatatttgg cttgagtgcc ccaagggcat
     4201 gggaagctgg gagttctttg actacctgct gaatgaaatt caggttgtcg gcacccccgg
     4261 tcagggcttt ggagaatgcg gagaaggata tttcaggttc tccacattcg gcagccccga
     4321 agatacaaaa gaagccgcaa gaaggctttg cgaattatta agatagtcac cctccattcg
     4381 ctgatgacga taccaaccaa cgggaaaaac cactgcttta cgcagtggtt ttttcccttt
     4441 atagactttt tatctgcact gttacatatc cgaactgtga gaaaataaat aaaaaagctg
     4501 agcctattgg ctcagcccat ttttttagaa cctcccgagg cgctttgccc cgggagggct
     4561 ttattatttt ataactctta ctctgtaaac tccctcgata gccttgattt cgtcaaggac
     4621 attctcaggg atagcgtctg cggtttcgat aagagctgcg gcggctttct ttgcgttgga
     4681 ggctccgccg gcgaccttaa tcgaattgtt tgcgaggatg ccgagaatct tctcggaaac
     4741 atcttcagcg gacaatacaa gcagtctttc cttagcgtct tcagacgggg caagctcaat
     4801 gttcgggaag tttacggagt ttataatctc acccttttca aggaatgctt taagctcctc
     4861 tactgccatg actgcgcagt tctcctcgga ttcgggagtg gatgcgccca ggtggggaat
     4921 cgggataact ccgtcaacgc caagctgtga gtcggagggg aaatctgtga cataagcagc
     4981 gcacttgcct gaattcagcg cttcaattat gtcgttttcg gctacaagct ctttccttgc
     5041 aaagttgagt atcctgacac cgtcacgcat tttctcaagg gtttctgccc ttacaattcc
     5101 tcttgtggag tcggtaagcg gagcatgaag gctgatgtaa tgggactccg cgaatacatc
     5161 gtcaaggttt gcggtaatct ttacggactt gtcaaggctt gtggctgcct tatcggtaag
     5221 ataggggtca tagcctacaa ccttcatgcc gagagctacc gctgcgtttg ctaccattac
     5281 gccgatggcg ccgagaccga taacgcccag ggtcttgccc ttgatttcgg gacctgtgta
     5341 gttagacttg cccttttcga ctaaagcaga gacctcgtcg cccttgccct ttaagctctg
     5401 gcaccactcg atggcgggga cgattcttct tgacgagagc agaagaccgc agataacaag
     5461 ctccttaacg gcgtttgcgt ttgcgccggg agtgttgaaa accactacgc ccttttcggt
     5521 gtatgcgctt acgggaatat tgttaacgcc tgcgcctgcc cttgcaaccg caagaaccga
     5581 ttcgggcagc tccatttcgt gcattgaggc ggagcgaaca attatagcat cgggagaatc
     5641 aaattcggtt gagcaggaat actttgcaga atcgaaattg gaaagtcctt cagccgatat
     5701 tttattcaga gttaaaactt tatacataga ttatccccca tatcagatag tgtataatcc
     5761 gctggaaatt tggaatacgg gattatttgt tttctgcttc gaactttttc ataaaatcta
     5821 caagagcctg aacgccttca taaggcattg cgttgtagat ggaggctctc atgccgccga
     5881 ctgtgcggtg acccttgagg tttacaagac ctgcggctgt tgcctctttt acgaacttag
     5941 cgtcaagctc ctcgctgccg gttacgaagg gaacattcat aagtgaacga tcctcgggaa
     6001 ctactgtagc cttaaagagg cttgaggaat cgagataatc gtaaaggagc tttgccttct
     6061 tgatgtttct ctctcttgct gcctcgagtc cgcctgtagc tttaagatgc tcgagaacca
     6121 gcttgcagat atatatggag tagcagggcg gtgtgttgta gagggagtcg ttctccgcca
     6181 tgattgtgta gttgagcatt gtgggagtga tgtcccttgc cttgccgagc atatcctctc
     6241 tgatgatgca gagagtcagg cctgcggggc cgatgttttt ctgcgcgcct gcatagagca
     6301 ttgcgaactt ggttacatca agaggctcgg acataatgca ggatgaaatg tcggcgataa
     6361 gcggaatgtc gcccacatcg ggaatttcgt ggtacttagt tccgtagatg gtgttgttta
     6421 agcagatatg aacatagtct gcctctttgt caaagtcttc tctcttggtc ttggggatat
     6481 atgagaaggt tttgtccttg gaggatgcaa ctattcttgc ctggccgtat tttgcggcct
     6541 cctggtaggc cttgtttgcc cactgacctg tgattataaa gtcggctttg cctgaaccgt
     6601 tcataaagtt aagaggaatg gcggcaaact gggtagatgc accgccctga aggaaaagaa
     6661 ccttgtaatt gtcgggaaca ttcaaaagtt ctcttacaag ctcttcggcc tctttaatga
     6721 ttgattcaaa aacctttgac ctgtgggaca tttccatgac ggactgtccg ctgcctttat
     6781 aatcaagcat ttcgtcggct gcacgctgta atacctcaac aggcagcatt gagggacctg
     6841 cagaaaaatt ataaactctt ggcatacctt ttgcctccaa aataaaaata aaagattgtt
     6901 ataaatatat caattttcca catcaaagtc aagtaaagac actatattct ttgggcaaaa
     6961 acagatattc gtataatctt ataaaatcag taatttatta gcgataaaat ttgctaaaat
     7021 agcaaaattt gcaggttaaa tccttgctac tccggtctct atggcggctt ttgaaaccgc
     7081 ctttgccact gcgggagcta ccctttcgtc aaatgccttg gggatgatat agtcgtggct
     7141 aagctcctcg tcggaaacaa ggctcgcaat agccatagcg gcggctaatt tcatctcctc
     7201 gtttatatcc cttgccttga catcaaaggc tcctctgaac acgccgggga atgcgaggac
     7261 attgttaatc tggttcggga aatcgctcct gcccgtgccc acaacaaggg cgccggcctc
     7321 tttggcaagg tcgggcatga tttcgggaac ggggtttgcc atggcgaaaa ctattgcgtc
     7381 ctttgccatg gtttttacca tttcgggcgt taggacattg ggggcggaaa cgccgatgaa
     7441 cacatccgcg ccctttatgg cgtcggcgag agtgcccttt aagttctgcg gattagtcca
     7501 gcttgcaaga tctttatggg catcgcttag actgtcgtct ccccttgaaa taatgccctt
     7561 tctgccgcac attattatat tttcgatgcc gagggattta aaaagctttg caatggcgac
     7621 gcccgccgct cccgcgccgg agaatacaac tgtgaggtct tccagctttt tatcggtaag
     7681 ctttaaagcg tttaacagcg ccgctacggt tacgattgcg gtgccgtgct ggtcgtcgtg
     7741 gaaaatcgga atgtcggtgc attctttaag ttttcgctct atttcaaagc atctgggggc
     7801 ggcgatatcc tcaaggttta tgccgccgaa gctgccgctg agaagggcta tggtctttac
     7861 aatttcgtcc acatccttgg agcggatgca aagagggatg gcgtcaacgc cgccgaattc
     7921 cttaaagaga acgcatttgc cctccataac gggcatgccc gcctcgggac cgatgtcgcc
     7981 aagacccaaa accgcggtgc cgtcggttac aaccgcgact gtgttccagc gtcttgtaag
     8041 atcaaaggat ttttcgggat gttccttaat ttcaaggcat gcggccgcga cgccgggggt
     8101 gtacgcaagg gacaaatcct cccttgactt tacgggagct cgggatacaa tctcaatctt
     8161 gcccctgagc ttttcgtgga gtttaagtga ttctttaaga tagtccatct atttttcaat
     8221 ccttattata atctctttac tcatctattt cgggggctgc ccatctcatg gacctttcga
     8281 ctgctctctt ccagcctgca tagagctttt ctcgctctct gtcgtccatt gagggctcga
     8341 aaatcctatc cgacttgcgg atatctctaa tctcgtccat gtccttccag aagcctacag
     8401 cgagtcctgc gaggtatgcg gctcccaggg cggtggtttc tacaaccttg ggacggttga
     8461 ccctgatgtt cgcgatattt gcctgaatct gcatcattat atcgctgacg cttgcgccgc
     8521 cgtcaaccct cagctcattg accgaaatgc ctgtgtcggc gttcatggcc tcgagaaggt
     8581 ccttgagctg gaatactatg ctctcaagaa cggctctgca tacatgggct ctgttgactc
     8641 ctcttgtaag tcccacaatg cagcctcttg cgtacatatc ccaataggga gcgccgagac
     8701 ccgtaaacgc ggggacgagg taaattccgt tggagtcagg cactgtttcc gcaagggtgt
     8761 cgcactcctt ggcgtttttg atgagcttga gctcatccct gagccactta ataactgagc
     8821 ctgcgttaaa ggcgctgccc tcaaggcagt aatttacctt tccgtcatag ccccatgcaa
     8881 tgcccgaaat aagcctgtgc ttggagcgga tgcgcttttc gccggtgttc ataagaagga
     8941 agcagcctgt gccgtatgtg ttcttggcgt cgcctgcctc atagcaagcc tgaccgaaaa
     9001 gggatgcctg ctggtcgccg attgcggagg aaatcggaac tccggcaaga gcctcgattc
     9061 cgggaacacc ttcggcgaca gtgccgtaaa cggcgctgga gggcataacc ttgggcaaaa
     9121 tctccattgg gataccaagc ttttcgcaaa ggtattcgtc ccagcagagt ctgtctatat
     9181 caaagagcat ggttcttgcg gcgttggaat agtccgttac atgaaccgcg cccttggtca
     9241 tattccaaat aagccatgta tctattgtgc ccgcaagcag ccttttttcc tttataagct
     9301 gctttgcaag gggtacattg tctataatcc acttaatctt tgtggcggaa aaatacgcgt
     9361 caagcaaaag tcccgtatgc tcttttatgt aatcctcaag accctctgcc ttcagctgct
     9421 cgcacatggc ggcggttctt ctgcattgcc atactatcgc gttgtaaacg ggcttgcctg
     9481 tttccttatc ccaaagcacc gttgtttcgc gctggttggt aatgccgatg gcggcaatgt
     9541 gggttgactt tatgttgccg gaaatcatgg cgtcgttgag ggatctgaac tggcttttta
     9601 atatctcata agggtcgtgt tcaacccatg agggctgggg gtaaatctgg gggaattcgt
     9661 actgggccat cgaaacgatt tcgccctgag aattgaaaac tatggttctt gagctggtgg
     9721 ttccctggtc taacgcaagc acatatttgc ccataattac acctcagtta attctttttg
     9781 atgatgctca gtgaattgct atgaatataa tactccaaag ggatagcact gtcaataaaa
     9841 aggggtcggg caaaaacgca gaaaaaaaga aagcagggga gatttctctc ccctgccctt
     9901 tattagagct tacgctccga tgaagctgaa tatcttgagc aggttcttga agaactcaat
     9961 aatcctctgg aagaacctga tcagaccgct tgatgcaaga ccgccatcat cgaagattcc
    10021 gccggagatc tgtctcagca gttcagcagc agcttctgca aagtcacgga ggaattcgga
    10081 atcggagttg gcgaagaggt cgaagatgta cttaacttcg gcattgaagc cgcctaagaa
    10141 gtcgttcatc tgaccaattg ccttgtttcc gccggagagc agatagaaga gtaccataag
    10201 taccaaatct gttccctcgg gtctccttgc atatgctgac aaggcgtcaa tcattgtgtt
    10261 gacggttgcg agaccagctc cgtcaaggcc ggttgcgtcc gcaagagcag ccttgagaat
    10321 ctcggtgttg tctctgaaga atacggtctg tacaaggaat ctgagcagga ttgtgaggaa
    10381 gtctcttgag tagccttcga tgtatactgc gtctccgacc actgcatcgc cggccttgga
    10441 ctggtaggtt gttacagtgc ctgctgcgaa ggtctcaagc tcgggcagtg ccgggactct
    10501 gaggcctgcg atgtcaaggc ctgcaacaag gtctgccagc atctcggata tgcttacatc
    10561 aagaccgaga tttacattgt agatcggacg gactgtgtca agcagtacgc atacgggctg
    10621 caggaggttg ataaggctta cattgaggag gtctgcgttg atgaagtaga agatgttggg
    10681 cagaatctcg tacaatgttt caacgggagc ctcataaatc ctgtcaatga gtgagaggat
    10741 cgggttgatg atgttcagca gcaggttagc gccgtcttca tcagcctgag ccttgtaggt
    10801 ttcgaagtca aggatttcgc tttcctcaat tccgagagcc tcaagaatcg ggataatacc
    10861 tgtgcggtaa ccctcgtaac cgtaaattgt ggtaaggctg agtaacggca ggtcatcgcc
    10921 aacaagcagt accctgagaa gcggctctac gggagcaagg aattcaacaa gtgccgctac
    10981 aaagccgtcc ttgtcgccat cctcgaagtc taaagcgagc ttgggattct tgaaggaatc
    11041 taagtcaaga ccgagcagcg gtgcgactat gtcaaggatg tctccgatgt catcgtcgct
    11101 aaggagctct gacagcatct cggctaattt ggtcatgttc ttcttggtgt aaacagactc
    11161 gccgaagagc ttatcgagca gctcctgaat gctgccaagg cctaagagct tgagcaggct
    11221 gtcgacgaac tcattcaggt tgtcagcaac gaagtctgcc ctttcagttg tccagtagct
    11281 ggagtagccg acctttacaa tctgattttc gggcggtgta agacctgcaa gctctgcatg
    11341 cgggtaagtg agtgttaagt tcggatactt tacggggttg aagagctcaa cgatagcggc
    11401 gatagcgtcg cccggattct ctgcaagacc ctggagtatc tctttcagtg ttccgtcaat
    11461 ttcaacagcg ccgggatcta cggggatcgg gttgccgttc tcatcaacag gaggtaccca
    11521 accgtcggta tttctgtcct tattggcgag catgttgatg agtgagccga gcatgtcgtt
    11581 aagggatgta agttcaattc ccagaccgcc catgagatca tcgggattaa tcatatcgct
    11641 gacatagatt gtctcgttta cgccgctggc aagaccggaa cccttaattg taatgactgt
    11701 gtcgaagagg tcagcaaagc cctggtcaaa catactgaat tccattgcgt aagccatgtt
    11761 gggcaggaag tcaagtatcg tcttgagcgg gttgttggcc atgttgacct ggatccagtc
    11821 aaagagcggg ttgaatatag ccctgagcat gttgaggttt acggtatagt ttattgtggt
    11881 gttgttgcag tagccttcga atgtggctgt tgaaactaca ccgggagctc cgatagcctc
    11941 aaggaagggc attatagcct tgtcataacc tctgttcctt gtaacattaa ggttgatgcc
    12001 tgcaaggaga atgctcctgc tgaaggtatg ttctctgttg ccccatgcgg ctttgaggag
    12061 agctgttaca ccagcaaatg cgcttgccat agcctggatc cactcatcac ggtcgccctc
    12121 cgggaagccc cagtcaagaa caacatctcc gtcctcgttc atgactttcg accagtcttt
    12181 gccggcagcc ttgagggcgg ttctgacggc gggatacttg cttgtgttaa tctgatcagc
    12241 aaggtcctcg gggaatacat taagtcccat tgcggcgggt gtaatgccga agaggcttgc
    12301 tattgtaccc attgtgccgc cgagctgctc gttaacggta ttagcaagtt cgggataaag
    12361 ggttccgact atggagttga cggaatcctg tgtgaacaag ccgttaatca ggtcatttaa
    12421 gatatcctta aagaccatgt aaatgacatt tccgggctcc tcgggttcgc cgggctcctc
    12481 gggttcgccg ggctcttcag gctcagcggg ttcttcctcg tcatcgtcac cgtcgaagag
    12541 accttcaaga gcggaaacaa cggttgaaag gaaggtttca tcgccaagag cgttaaggag
    12601 atagttcagt actgcaacca gaacatctgc tctgtcagcg ataattctgt ggcctacgcc
    12661 agatgacctt gtagaactaa agctgataag ggtgccaagg cttgcgagcc ttgcctgatt
    12721 gatttcgggc agtgaaacat taatgggagg tacgggaggc tctgtttcaa caggctctgt
    12781 ggtgccgggc tgtgtggttg tgggagccgt ggtaccggtt gtagtaacgg tggtctcctc
    12841 aacaatgccg tagtcaataa gttttccgaa ctcctggcta ttgaggaatt catcaagctt
    12901 atcaattaca ttaaggagtc tatcctttgt gaccacatag tcatggtcgg ggctgattat
    12961 tctgattata tcgtcttccc tgacgacacg aacaggcagt tcaggctcta acttgtaatg
    13021 tccgcctgtg ccgttaggtc cgacaagacc cttctttgcg acaaagtcgt tatactccgc
    13081 aagtgcgggg tctaaccagt tgtatttgct ctgaatgctg gagttgttgt acggtgtgtt
    13141 ctgaatgagc ggatagtact ctctctcaac cattccgata agctctctta cttctttgta
    13201 gttgatgagc ttaattccgc cgtaatcata gtagcgttct gcggcaatat tgagctgagc
    13261 ctcaagtctg tctgcgattt catagccaag gctgtctatg taagctacta ttctgtcata
    13321 gtactcgccg aagattctga tgcggatctc aaggtcataa gcattaatct gctgcagctt
    13381 ctgacagcct gtattgaact cggcaatgat tgttgcctta tccataagag aggcatcaga
    13441 tgcaaggcga gactcgttga agtactcgta gtactgaccc gattcgtcaa ggagatttct
    13501 tgctacaagc tcctcattga gaagggcaag atactcgcct acataatcgg ttccttctgt
    13561 gaagattgct acctttgcct ctgaggtgta gtatggaacg ccgcttgcgt tggtgcctac
    13621 aacagcgttg tactttgctg taaactcgtt aacaaaggcg tagaactcgg tattggagag
    13681 tgagccctct tcctcaaagg ggattaccaa gttggcgatt acatcgtcaa agtaatcctt
    13741 gtactgctgg agctcgatgc attcaatatc gtgattcagc tctctgcgga aggtgtcgat
    13801 tgcgttaaag tcaagatcgt agtgagcctc gaagaactgc cttactgcct ggagagcgga
    13861 gtctgtgcgc agaacattaa ggcgattgaa tgctgcggtt gatgctgtgc gcagctctct
    13921 cttctgctca agtgtgaact ccgaatagtc gagagccaat acattattaa agtataatac
    13981 atcaggccta taccttgaaa tatcggcata gtacttaaga tttgcaacga atgctgctgc
    14041 ggctgtaaga gtaggcagac cgaagtgctc cattatctca ttgtcgaaac ggttgcctct
    14101 ctcaagcgct gcgactgctg cgtcatagag gtctacgatt tcctcgtctg taagggtgga
    14161 gaggtcaata ttcaatgttt cgggagtgaa tacacttctg tatgcgtcaa gatctgcctt
    14221 aacattgttc tgaggctggc tttccgcata gttgatagca gtaagataat gccagcgata
    14281 tgtctggaat aaaccgccgg atgccgatgc aacggagtgt acccaagtat atgtagctct
    14341 gaggtttatg gtattgggca catcgtcaac tgatgcatag gacagaaggg cagcctttaa
    14401 agtacggttt accgtaaatg tgcagttctg cgcgttaagg ctgcctgttg aaccgacttc
    14461 cctaatgtta tacgggttac cggcaaagta ctgcatcagt gtgtttacat tgtactgtgc
    14521 gtattcctcg ctggtgaaat aatcggggct gttaagctga ttcctcagcg tcgcgattac
    14581 ttcgggaccc gtattgttgt cggtattgtc gtttctgtca cccgcggtgt tgagtacagc
    14641 ggtagacagg gcggcttgat agaatttgta agccgcgtcc caaataccgt tcgtggtgtc
    14701 actggacacc gtcacactgt tgctggaaac gctgtagctg ctaagagtgg agacattggg
    14761 ctttgcttta attgcttcag ccagagctgc aagctggctt gcggaagcgg caaagcctac
    14821 gaatcctgcc gagaagcagg acaggatcag gacaaccgtg agcataatac tcatcggttt
    14881 ttttgagtcg cttcattcaa tcactccttt ttctgcagtt aaactaaata gttttttagg
    14941 caaaaaagag cctaattttg gacttaattg ccattagttt cagtttattt tatacactaa
    15001 caactgtaaa ctaattattt tcaagatttg tgaaaatctt gaaaatatcc caaattcaat
    15061 taacaaaatt ttcataattt gcgcatcaca aaagtcgctt tttgcaaaat aaccgattat
    15121 tgcttatagg taaatatttt ctgattcatc ttcggtaaaa ttatataata attctgaata
    15181 ttcgacaacg gaaaattaca gcaaaactgc cgcgcaaaaa gcacggcagt ttcgctgtaa
    15241 gattcataaa tcagtcaagg tcaatattgt agaatgccag cctgccgggg taaacggcgg
    15301 gtctgccgat ttcctcctct attctaagga gcctgttgta ctttgcgacc ctctcgcttc
    15361 tcgaaggcgc gccggtctta atttgtcccg cggaaagagc cacggcaagg tcggctattg
    15421 tggtgtcctc ggtttcgcca gagcggtggg agattaccgc ggtgtagttt gccctttgag
    15481 cggtctttac agcgtctatg gtttcggtaa ggctgcctat ctgattgagc tttacgagaa
    15541 tggaatttgc ggtgttaagc tcaatgccct ttttgagcct ctttgtgttg gttacgaaca
    15601 ggtcgtcgcc aacaagctgg agtcttgagc ccagtttctc ggtaatagcc tgccagcctt
    15661 cccagtcctc ttcgcccagg gggtcctcaa gagagataat cgggtatttg tcgataagac
    15721 tttcccagta agaaatcagc tcgtctcttg tgagcttctt ctttttcttg ggcataaagt
    15781 atgtgccgtc gtccctgtac cattcggtgg aggcggcgtc aatggctatt ttgaactcgg
    15841 tgccgggcat atagcctgcc ttctttatgg cttcgagaat cgcgtcaaga gcttcctcct
    15901 cgctgcccag gttcggggca aagccgccct cgtcgcctac gccggttgcg agaccctggt
    15961 cgtcgagtac ctttttaagg ctgtggaata cctcgcagca gcgtcttaat gcctcggcaa
    16021 agctgtccgc gccgatgggc attatcataa attcctgaat gtcaacattg tttgaggcgt
    16081 gggcgccgcc gttgagaata ttcatcatgg gaacgggcag ggttctggcg tcgcagccgc
    16141 cgatatagcg gtaaagcggt attcccagag tgtttgccgc agcctttgcg gcggcgagag
    16201 aaaccgccag aatagcgttt gctcccagtc ttcccttatt ttccgtgccg tcaagctgaa
    16261 tcatggtaag gtcgatgtcc gcctgattaa aaacgcacat gcctttaagc tcgtcattta
    16321 taatgccgtt tacattgtca accgctttgg aaacaccctt gccgccgaat ctgcccttgt
    16381 cgccgtcgcg cagctccaca gcctcaaagg caccggtgga cgcgcccgag ggcactgcgg
    16441 cggttccgat aacgccgttt tcaagaatta catccgcctc tacggtgggg tttcctcttg
    16501 agtcgattat ttcccttgcc cttattctct gaattgaggt tcctgccatt taatcatccc
    16561 cttttaaaag tatctaaaca aattatatct ttgccagtat ttgcattaat atgtaaggta
    16621 ttttaccttg acagtaactt taatatgtaa agtattttac cttgtcatta tttttcactt
    16681 tgggagaagc agttagcaaa ggacaacttg aacgcaaaaa ataaaaggca gaaaagaaac
    16741 taacaacatg cttcaatttg ccgttaattt acaaattcta cctttattat aagtaaacct
    16801 ttttaaataa tcaacagcat ttaagtttat atttccgaca gaatgctctt tatctgcttt
    16861 tcgcagttag aaatgagggt tttaaacacg agtatgtcgg gaacggactc tgactcctcc
    16921 gacagttctt cctttttgaa actatcggaa aaatagtttg ccatggcgtt tgagatattg
    16981 gtgcccgcgt cctctgagag cgagaaccag tcatcgggca cggttcccgc ttctcttgcc
    17041 ttggaaagaa ggtgataggc gctgaaggca aggtaccatt ttacgctctc cgcctttttg
    17101 ccgcccttgg cggtcattat ctcgccgagc tttattatgg ctctgtagat ggtctgcatt
    17161 ttcatgtcct catcggaggg cagctcgctg ctttcaaaca ggtcgccgag tcccctgcgg
    17221 ttttcgctca aacccagaag ataatctgcc gtaacatcat agtagttgct ggctttgcgg
    17281 ataaagtcaa gcccgcactc ccttattccc ttttcgtaat gggagagaag ggcctgggac
    17341 acacccagag cctcggcaac ctccttttgg ctgactcccc tttcgtgcct taatgaagtt
    17401 aggcgggaac cgaattcaga actcataagc tcctcctttt gaagaaactt ctattacagc
    17461 ttgcagacat ccacccagcc cttagcgttt gagtaaaact ccagctccgt aagattcagc
    17521 tcaatcgcgc tgttggagct gccgcaggcg ggataaacgg tttcaaaacg ctttaaagac
    17581 tggtcgaggt atacatttac gccgtcgttc acatcaaagg ggcagacgcc gccgacggga
    17641 tagccgacaa gctgttctac ctcctcggcg ctgagcattt ttgccttgat tccgaactga
    17701 gctttgtact ttttattatc aacacgggcg tcacccgcgg ctacgaccat aattactccg
    17761 tcatcggact taaaggtaag gctctttgca attctctccg gcgggcagcc cagagccttt
    17821 gcggcaagct ccaccgtcgc gctggagaca tcgaactcca atactctgtc ctcgcagccg
    17881 tgttttttaa agaatgctct tactttttcg atagacattt tgacctccgg ttcgctgatg
    17941 tattaattct gctcgcttaa aatttcttcc agttggagat attccgccag cagttcttct
    18001 tccttttccg ataatgcgga cagctttgca gtcagctccg ttaaccgctc gtagtctgag
    18061 gcgacttcgg gggattgaag gagggtgttt gtttcctcca gttcttttga tacttcctgc
    18121 agctccgctt ctattttttt gagcctcgtc tgacgcttgc gcctcaggct ctcctcctct
    18181 tttcgtcttt tatactcatt cacctttttc tccggcttag aagattcggg ggccgtgccc
    18241 tgctgaaagc ttaaatatga gtcgtagttg ccgtctattt ccttaaccac gtcagctgta
    18301 aaggcaagga ggttcgtggc gagtctgttt atgaaatatc ggtcgtggga aacggcgatg
    18361 atggtgccgc tgtagtcgga gagagcctgc tccagagcct cccttgacat aatgtcaaga
    18421 tggtttgtag gctcatccag gataagaagg ttcgggcgct ttagcataag tttcaatagc
    18481 gccagctttg ccttttcgcc gccgctgaga gagcctacct ttttttctat gtcatcgccc
    18541 ttgaaattaa aggcggcaag gtaatttcta agctctggga tagatttgtc aaggcaggaa
    18601 ttgtagactt cctcaatgca ggagttctca tcgttaagcc cgctgagagt ctggtcaaag
    18661 tagcccacga ctatgtttgg gccgtagaaa accgcgccgg tgtcgggggt tagatgcttt
    18721 gctattattt ttataagcgt tgattttcca cagccgttgg gaccgactat catcagcctt
    18781 tcgccctttt tgacggtgag agaaagattt gaaaacagtc ttttctcgcc atagcttttt
    18841 gagatttccc ttattgtgag aacctcgtta gggctttcga ttctctcctc gaatttaagc
    18901 cgcatagcct ttgaaggggg gactggcggg tcgccgagat tttctttcag cctttctatc
    18961 tgcttttcgc ggcttgcaat tacaatgtaa tttcgctcgc ggttaaatct tctctgctgc
    19021 tctattatgt cttccagcct tttgatttcc tttagggttt tctcgtactc cctttgctca
    19081 gacaaaaggc gctcttcttt aagctccata tatcttgtaa aagacccttt gccgagatac
    19141 agcttttttc cgtatatctc aagggttttg tcggttaccc tgtcaaggaa atatctgtcg
    19201 tgggagatta caataaaggc gcccttgaaa ttctgcaaaa agccttcaag ccattcgacg
    19261 ctgtttatat caaggtggtt agttggctcg tcaaggagaa taaggtcgga gtcggaaaga
    19321 aggagcttgc cgaggctgat ttttgtcctt tgacctccgc tgaggttgcc gacggggctg
    19381 tttatctcct cctcggaaaa cccgagaccc aaaagggctg accttgccat gtttttatag
    19441 gtaagaccgc cctctctttg aaaacgctcg gtaagggcgt cctgctttgc gataagctgc
    19501 gacagttcct cgcctttggc ggcgtccacc tttgagtgaa gctcgtttag ctccttttcc
    19561 atttctgaaa ggtgggaaaa gacagtcagc atttcttcat aggcggtttt gctaaggtcc
    19621 ctgcaggcgt gctgttcggc gtagcctatt ttggtgctct ttgaaataat aacctgcccg
    19681 cttgtgggct caagctcgcc gcagaggagc ctgaaaaggg ttgttttgcc gctgccgttt
    19741 tcgccaataa agccgatgcg gtcgttttcg tagacattaa tatttgaagc cgtaaacaac
    19801 agcctgtcta aaaagctcat ttcaagattt tgcagtgaaa ctaaagccat tttttctccc
    19861 tgaatacacg ccgcggaaaa cggcaaaatc gtcacttcaa aatcttagca cattagtata
    19921 aataattcaa tcaccgcaca catcaaataa aaacggcggc tattaagccg ccgtaagttt
    19981 gtttttgttt agctgaagat aaattcctga gttatgtcta tggttatctt catatccatt
    20041 gtgaatgtac tggtttccaa aaactccatt ttgtatgtgg caaccgcctg gcaataatat
    20101 gtagccttcg tgagattgcc ttcggaatcc agctcgtaga aaaggctgcc gccgttgtat
    20161 ttaagactct tgctcttaat tttaatcagc ataccaaggt cgtcgccggc gtcatccatc
    20221 agatcaaagg ggtcattcag cttcgcaaag tcttttacgg gaagtgcttt tgtatagacg
    20281 ctgtcgttgc tgaagttttc aggattaaag aacagggtgc attgctgatt ggttttaagc
    20341 atgccgtaaa ttgtctgggc atcaagagga atatccacag cgtttgagcc ttcgggagga
    20401 agtgctttta caaggccatt tgagtcaaga taataggtgc tctgatcggc gggataggtg
    20461 aagccttgtt caagctcgct cgggtcttga ccaagtgaac gcaggaaaag ctttaatgcc
    20521 catgaaagag atatatcggc ttttgtgttg attgtctgtt caactttggc gtttttttga
    20581 acttcatttt tttttatttt tgttgcagct gtattgtaag ctttaattgc gtccttgatt
    20641 gcctggtcgg cttcactgcg gtatgttaaa tcaatattta cggacatatc aagtcctgcc
    20701 gcaatacgaa ggatggcacg ggcatcaagg ggcgttacat gcccgtccat gtcaacatct
    20761 gcagctttca gctgatctga gttaaatgat tcaagctttg cgcagtaacg cagaacaaga
    20821 cgggcgtcgg cggcggtaac atcgcctttg ttatccacat cgcctgtgtt gggcttacct
    20881 gcaagatcgg gtttaagctt taccgcggag gcaaagggtg cgtatgccgc aagcattgca
    20941 aacacaagcg ctaaagctat taaggattta atcttcttca tattcaacta cccctaattt
    21001 aaagttaagg taaagtaaga cttaaaatta tataaaagtg caaattcagg ctgcttttat
    21061 attcatcata ataataacat acaataatat gtttgtaaag aacgcaaatt caaggtatta
    21121 tttacaaaga aggtgtgtct tccggctccc ctattacatt tggacaatat tcgactttcc
    21181 tttgacttaa aaacggattg tagaaaaatc aatactcatt ttctgtagca aagttacaga
    21241 atttattaaa gtaaagggat aaaataaatg gcgaatcgga aaggtataat ttttcctttt
    21301 cggtctaagc tgttaaaggc aaaaaaccct tatgagagga ctgattttgt gaatagggtt
    21361 gctgttgttt attggagcgg cacgggcaat accgagaaga tggcagagct tgtagccgaa
    21421 ggcgtcgtcg ccgcgggcgg cagagcggat attttcaccg ccgcagagtt taccaaggag
    21481 atggttcaaa actacaaggc tctcgcgttc ggctgccctt ccatgggagt tgaggagctt
    21541 gaggatacag agtttgagcc tatgttctca tcggtgaagg agtttctaaa aggaaaagtc
    21601 ataggtcttt tcggctcata tgactggggc gacggcgagt ggatgagaaa ccgggtcgcc
    21661 gactgcgagg actatgggct tactctgatt aatgacggag ttatagcaaa caacgagccc
    21721 gacgatgagg ctgcggagaa ctgcagagct ctcggcgcgg cattggccaa ggcgtagtta
    21781 ttatttttat aaaaaactgt caaaattgta aaaattcttt atcaattaaa gattgattta
    21841 agctaattaa agttatatta tggtaacata accacacagg gttctacgga gggaaaacca
    21901 atggaagttt cagatattat gcgcgcgaga acatcgagaa ggagctatac ctcctcaccg
    21961 attccaaaag actctattga aaagctccag gacacgattg agtatttgaa cagaaagtcc
    22021 gggcttcata ttcagcttgt gcttgacgga gaggcagcat tcaaaagctt taaaaagaca
    22081 tacggaatgt tctcgggcgt aaagcattat tttgccatgg tcggaaacag cagcgaccct
    22141 ttgctgcatg aaaaggtagg atattacgga gaatatcttg tccttgtggc tacagagctg
    22201 ggactcggca cctgctgggt aggctcctcc tatgacgcgc aaaactgcaa ctgcgagatt
    22261 ctcgccggcg agcagcttgt atgcgttatt accgtaggca agtctcccga gtcgctttcc
    22321 agcagagaga gattggtgct ctcaatggtt aaggaaaaga gcaaaaagta cgatgagctg
    22381 tgccaatatg acaagcagcc gcccgagtgg ttctgggacg gcatgaaggc tgtggagctt
    22441 gcgccctcgg caagaaacag gcagcctgtt cagattattt actacaatga tgtaatctac
    22501 gccacaacgg gacagttcca atataataaa attgatatgg gcatagcgaa actccacttt
    22561 gccctgggcg ccggcggaag gtttgagctg ggcgaacacg gcaggttcta taaagacgag
    22621 gaaacagtat aattaggaat aaattaagag ccgcttaaac agcggctctt ttttagctta
    22681 aggtttcgtc caccggttct tccgcgattt ctttcagggc ttcgagctct tccttttcaa
    22741 tcttaacctt cgggttcatt atgagcttta catccttgca gtgataggtc tgcggagcgg
    22801 cgtccctttt ggaatcaaga attaccttga gaacgcctgt aagcagattg ctctccacga
    22861 cctctcccct gccgtcggcg gtttccacaa tggcgccgac cttcggagta tgtcttgcaa
    22921 ggtgctcata cacctcctgc tcgtatttaa ggcagcacat gagtcttccg caggagccgc
    22981 tgattttgac ggggttgagt gaaagtccct gttcttttgc catctttatt gaaacgggct
    23041 caaagtcgct taaaaactgg ctgcagcaga agggctttcc gcaaatgcca aggcctccga
    23101 gcatttttgc ctcgtccctg acacctatct gcctgagctc aattcttgct ctgaagatgc
    23161 ttgccaaatc ctttacaagc tccctgaagt ccacccttgt gggagcggta tagaaaaaca
    23221 gtattttgct tgagtcaaag gtatattcaa catcaatgag cttcatttca agaccgtggg
    23281 cctgaattct ctcctcacag attttgaagg ctcttttttc tttttcttta ttctcctcta
    23341 caattttaag gtcctcaggt gttgcctttc ttataatgct ttttaagggc tttacaatct
    23401 ggtcctcgtc aacatcgcgg ttttccatcg caatttcgcc gcattcaatg cctcttgcgg
    23461 tttccactat tacattgtcg ccctttttga gctgtactcc ggccgggtca aaataataga
    23521 ttttgccgac taccttaaat cgtacgccta ctacttctgc catatatgtc tccttaatgg
    23581 attaattctc gtttgcggtc attccgattt tatcctcaga cagccttgag caaaagaatg
    23641 ttgaaagcaa ggtttcgttg gcgttcattt ccgccaattc ggctatcttg gacagggtgc
    23701 ctgtccacct aagtaaaagc gacgggctta cggatttata catttttacc gccatagcct
    23761 gggattcgtt ttcaggatag ccctcggttt ttgcaaagcc cctcttttgc agcttcagag
    23821 cctctataaa gatgcccgtc agttcatcgc agacattctt tataagctgc ctgtcctttt
    23881 ttatgggagc cgccgctaat acaaggtcat actcgctgcc cgaataaagc gctcttgcta
    23941 tgtcgcaggc aattgtctga gccttgttct gctttgactc ctgcgattcc tcgcccaaat
    24001 caaaggtgcg cacccttgag cggattgttt caagcagacc ctctgaggag gctgcggtaa
    24061 ggataaagcg ggcgaaacgg ggaggctcct caaggatttt gagcattgcg ttctgagctt
    24121 cttccgtcat gttctgcgcc tcaaagacaa tgaagatttt ctctgaggct tcgttgggca
    24181 ttatgagcgc ctgctggcgt attaataaaa cgctttcttt ttttatgctc tttgctttgt
    24241 cctcgccgga aatctcggtg atatcgggat gaagattttt cagtgacttt acgcagtggc
    24301 cgcacttacc gcagggtttg tccctgtctc cgacacacag cagggctgag gctgtcagcc
    24361 ttgcgacctg ggctctaatc ttttcagtcg ctcctgtaat cagaacggcg tgggatagcc
    24421 tgccgctg
//
LOCUS       DAONVQ010000010        24388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_1420_length_24388_cov_4.626762, whole genome shotgun sequence.
ACCESSION   DAONVQ010000010 DAONVQ010000000
VERSION     DAONVQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 24388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24388
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1420_length_24388_cov_4.626762"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="PLD98_01490"
     CDS             <1..1389
                     /locus_tag="PLD98_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04959.1"
                     /translation="TELEGNIIAGATDYNGIDWFITDRYLYTNYERGTLYPYHETETP
                     DCKGLAAFNEDFVFAAGSFGLQIIHGKRPRWATLITQTSKIPTNELTAIAADTWNHVW
                     IGTKNGALIFDGRNRWLTPDTVKALPRASVTTIAIGKNGAAYIGTEIGLYIIDGTKES
                     FLGYNRWLPDFKVTAIAVSSDSDTLCIGTEKGLAIIEFKLMSLSEKAEYYQYTVEKYH
                     NREGYTTNRQLTEKGNIESGAPQITDNDGLWTGCYVASQALRYGATKDKKALESARKG
                     MRAMLKLMSITGIEGFTARAYRRPGEPGFGDGDKEWHLTEDEFGPLEWKGETSSDEMT
                     GHFFGLSLYYDLCADEKEKKEIALALSNIMDHILSHEYTLCDVDGLPTTWAHWGPHEL
                     NNDEKWFWERGINSLELLAFLKIVYHMTGDEKYQKEYEKLIRTQNYALNCVQYKIFDA
                     HTSHIDDNLGFL"
     gene            1390..1821
                     /locus_tag="PLD98_01495"
     CDS             1390..1821
                     /locus_tag="PLD98_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04960.1"
                     /translation="MTSLLRYEQDPTIRQYFLMGFKHHWNDQRIERSPLWNIMYGALT
                     GEHCDLENAVRSLEELPLDLIHHKVVNSVRPEIVWDSGQELFGGVRQLKEPLPYDEKP
                     MNKYDNNPFLADRGDGLRAEDGTVFLLPYWYARYYGMIIEE"
     gene            1920..2822
                     /locus_tag="PLD98_01500"
     CDS             1920..2822
                     /locus_tag="PLD98_01500"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HQA04961.1"
                     /translation="MSFSDYAKNFGGVYSLMLTPFKEDRSIDYDIYPEYVRWQEEQGA
                     HYLFAVCGSSEMKNLTLEEREKLAALTVKHRKKVKVFATANLEPSWHGQLEEIKRIEA
                     AGVDGLVFVTKGYGNDEERLVTYLLELQSHATVPVILYEFPGFSNHLISGSAYKRLVE
                     NGNFHAIKDTTCTMQGIKEKIAVQGDSCVLQANIPYLLEAYEAGARGVAATPTTCGTN
                     LFIRMWDSFAAGDMEEARRMHEEIVNLDNALGGAFPATAKYLVNLQGIPMNWYTRGDH
                     VLKDHKLKALRVYHDYAVRNNLMK"
     gene            2839..3744
                     /locus_tag="PLD98_01505"
     CDS             2839..3744
                     /locus_tag="PLD98_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQA04962.1"
                     /translation="MEKYLIINADDFGMCRAANLATFDLFEKGGISSATIMMPCPWAP
                     EAVKWAASHPEYAVGVHLTLTSEWKYYRWGPVAQSGTDSLRDEYGYMYPECDEFEENC
                     DIDEVETEIRAQVALAKRLGLNPSHLDNHMGSLYGFNGNMEVMPRTLEICGELGYAFR
                     LFRSLRREFIPSTFPQDLPLDLLNMLVAGYVSIADEKGVPLIDNILFPEWTEDGKADY
                     ETYREYMLTRLANIPEGEITETFIHPSLECDELKGITSLWHCRVWEHRLFSDPKTRQH
                     LESHGVKYINYRDVVRMRKEQASKE"
     gene            3879..3955
                     /locus_tag="PLD98_01510"
     tRNA            3879..3955
                     /locus_tag="PLD98_01510"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3913..3915,aa:Ile,seq:cat)
     gene            4021..4096
                     /locus_tag="PLD98_01515"
     tRNA            4021..4096
                     /locus_tag="PLD98_01515"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4054..4056,aa:Trp,seq:cca)
     gene            complement(4342..5973)
                     /locus_tag="PLD98_01520"
     CDS             complement(4342..5973)
                     /locus_tag="PLD98_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04963.1"
                     /translation="MSDFVFFHGYDSKLWDCFVKRGIINSNAGIRFCQNLMLPEEKKF
                     NRLMKEDKKLKSLVHDERLPVYIDRLQGGAYIDTCRYDMELIEALEDRFYGFQMHEWM
                     SNLRTDYNKLSRVKDEEWNKETIEKEIFRQFPFKHLFLEAATAEEFAIELKRPKTFEE
                     LLINAEKLYRKRLNETGGRLIPCDSAMMAANLEFKLGAKRVMPEIGAQTPLANIQLSY
                     ARGMAKAYKGSFGAYYEPWGGKPFSVCTYFKDGINEWNIKSEGDFPFKALGGEGGSSR
                     SLQRRIYIYSYLAGASFLSEEWGLHNTFIDDENFELSPYGLVKKEFLSFISKYDQGKF
                     YAPIAIVLPKDMMALANVYNSSFNQSLEMPYENKGTQERDKKIAEKIRELMGQSASGF
                     GNEYRTLPNSDIPDAFDIIHEDCESVLGEYEYLVDLSFNPAFKKKHGNIMSPKEAVEK
                     ISELMPCRVSGGLHWFVNRLKNGWYLTVFNNDGIEKSVEYGEKANPEAERTAKVELKS
                     GLNLLPLEGSKEIKRIDGKFFVTLKGGDIFFAKLV"
     gene            6116..6439
                     /locus_tag="PLD98_01525"
     CDS             6116..6439
                     /locus_tag="PLD98_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQA04964.1"
                     /translation="MNIFVLNGSPKGEYSITLQTVNYLKLFFPEQNFETYHVGHKIKA
                     LERDMSPVIDLINKTDLLLFSYPVYTFIAPAQLQRFIELLKQSGFPLRANTPPRLPPQ
                     SVFMM"
     gene            6436..7497
                     /locus_tag="PLD98_01530"
     CDS             6436..7497
                     /locus_tag="PLD98_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HQA04965.1"
                     /translation="MTAHSYIEENCHDLKLKYIAGLSADMDDLLSVNGQKDAREFFEY
                     VLWCAENDYYVKSPSVSCSYTPAPGTVPDDPSEPKTGDVVIVTDCEENDTQLKNMIAR
                     FRAVLPRKTRVVNIREYPFKGGCLGCFNCAVSGKCVYKDGFDDFLRNEIQSAEAIVIA
                     FTIKDHLMGSCFKMYDDRQFCNGHRTVTKGMPMGYLVSGALEAEQNLKTIIEARARVG
                     GNFLAGVASDESDPDSEIDKLANTLEYALQHKYAPPGNFYGVGGLKIFRDLIWLMQGM
                     MRADHKFYKKHGMYDFPQKKKGTILKMYAVGMLLAAPKVKAKMGNMLNEGMLMPYKKL
                     LEKQNQTYLLNKAILSFSQ"
     gene            7582..7989
                     /locus_tag="PLD98_01535"
                     /pseudo
     CDS             7582..7989
                     /locus_tag="PLD98_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376297.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            8181..9416
                     /locus_tag="PLD98_01540"
     CDS             8181..9416
                     /locus_tag="PLD98_01540"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HQA04966.1"
                     /translation="MVIEHFVENEKEGQYYTIPFSVPQNVIKITVKYEYYRPTKGILS
                     DLRPTNCIDIGLMNEKGRFLGWSGSSHSEISVGEYSSTDGYITGKINPGEWRIIIGAY
                     HVEKKGVTVKYTIDFEFAGEKLLFGDLHIHTKASDGIFDVSQIANMAREKGLDFIAVA
                     NHNNYAENFALPAVNGLTYIPAVEWTHYKGHMNFYGVKNPFEDSFIANSIEEMRKVIE
                     KAKALGAVISVNHPKCAIIPYLWEDDYIFDMAEIWNGPMRPTNVRGIAYWTSLLKAGR
                     KVVAVGGSDYHRPLRFVKIGNPVTGVYSPSQSAEDILDSIKKGRCFVSESKDGPAIDL
                     KYGGFRMGDTAEYKEGTDLTVEVKSRYKVKLYLVTDKYEKEIKAGKVPLDKVKFAYIK
                     ATKKHGNKVRAVSNPIYFN"
     gene            9431..10741
                     /locus_tag="PLD98_01545"
     CDS             9431..10741
                     /locus_tag="PLD98_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA04967.1"
                     /translation="MAAESRLAKRTSPSRYGFAMFGTSIPINMFKSFAAIYYVDMLGL
                     DMKKYSLVLFIYTFVDAIDNPVYGFLSDRTRTKWGRRRPWLLIGTPLLVLSFILFYNV
                     PAFVQTEKGMLFIYMLLMYILTGTLDSLVNANYGALFPELFKDDVQRAKVNGFRQICQ
                     LFAMAISIALTPMITGAIGYRLTSLIYGILALAVMMYCFFGCHEDLEYIEDLEKPSLF
                     SSVFALISNPKFWIFGFVGAFYSAAFSLISQAVSFYVKYTLGLGGNVTTIMLGVVLVV
                     AVSGIVFWSWILKKWTVMKVWRTGLIIMAVSFLPLFFINSLIPAIIAACFVGFGVGAC
                     LTTMDCIGAKIVDDDYAKHGIKREGIINSLMGVMNRLYGLYTSLAFYIVSVTYDFVNG
                     ENPGPNPAMASRVLLCLFPAAAMVLASIISFFLKFPEMDKRDKS"
     gene            10753..11619
                     /locus_tag="PLD98_01550"
     CDS             10753..11619
                     /locus_tag="PLD98_01550"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HQA04968.1"
                     /translation="MSRFLIINADDFGCCKEQNEAIKELWENNLITSVSVLAVAPEFE
                     DAANWLKSKGLSAGVHLTLNSDEALNPWRSITGAKSLGKNGNLHSNSKDITFHARRRD
                     VSREIAAQYNAIKNAGIKVDHADSHSGTLYGLNLRRFYIDAFRFCGENGLPYRFPKRA
                     DFIERMLGRKPPAALYKFHSHLVNLAKKNGVCLIDDMLSNPWSIDKIKDKDSILEYYL
                     NAVKGIGEGITEIFLHPAKPKSEEKTPWTKRVYEYEILKSGALLEAAKKNGIEIISWA
                     DVREINNYRRTE"
     gene            11616..13073
                     /locus_tag="PLD98_01555"
     CDS             11616..13073
                     /locus_tag="PLD98_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04969.1"
                     /translation="MRKFVSIVLAIVLMFSVCMPAFAAGSVGAENEPTPPEYPLIVIR
                     GVNFMNEYLDVGTKNERIYIPPIKASDIFAAVFKGAAAYAVTSDKKKFLTPVLDYLNE
                     TFKYIACDKNGDSVYNVGVYEYPLSAANYEDFPLGDDHEEGILTEAVNRYGAEYVYYF
                     NYDWRIDPLDAADKINEMIERALLDHGCDKVNIICCSMGGVMTVGYLYKYGYDKVNKI
                     VFLSAVLDGTYMVSDVYQGKINFNPVYAYNFLREGLGLPFIAKILVDILYKAGVIDRL
                     CELLNSFVAEETQFVLDYTVRDIFGTVSSWWALVIKEEIDDAVNYMFPTDEMKEEYSG
                     LLQRIERIKEMLYAKDELLRNAKADGVGIFVLASYDKPLFPFYERSGSIGDGLETARM
                     AGGAITAEYGKTLDVEESEFLSPDKMVDVSTVLFPDSTWIIKNAPHVACKVGSDFAEF
                     MFCLIDTEENMTVYSNPRYPRFLCVDKNQNFVPYK"
     gene            complement(13132..14580)
                     /locus_tag="PLD98_01560"
     CDS             complement(13132..14580)
                     /locus_tag="PLD98_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HQA04970.1"
                     /translation="MAKERIHGYLPDEKPPVGKLLLYALQQVIVMFPATITVALITGF
                     HVSTTIFASGLATLCFVLITGRRIPLYYGSSFAYLSAVIGLMSSETVTNALSADEIAQ
                     LNGFLNGEAGFFTMPTATIRYAQVGIVMSGLVSIAAGLLVKFVGIKAVEKILPPTITG
                     PVAMIIGLTLAGNALADAAPNANATGVFASNWVWVISLVTLLSTILYSRYLKGFLGQL
                     PLLLGAATGCLAAAILFWIGGADYNMFRVISADAIESLWSAGPFAIPKFSLPDFSNSK
                     IALASIAAIMPIAIATIPESTAHMYQLDIYVNDVAEKKGAKKYNLIERLHMNLIGDGI
                     GDMITGFIGGPAGTNYGENISTMAITKVFSVPVLMVAAVIAMIISCFTPLIQAIYAIP
                     LAVIGGLEIYLFGAIAAQGVAIMIDKNADMFSSKNIAVIASIVIIGIGGQYVYGGNIP
                     FFGLNIPCIAGAAIFGIFLNAILSIGEKKKAE"
     gene            complement(14904..15536)
                     /gene="upp"
                     /locus_tag="PLD98_01565"
     CDS             complement(14904..15536)
                     /gene="upp"
                     /locus_tag="PLD98_01565"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQA04971.1"
                     /translation="MERQVVILDHPLIQHKLRFLRDENTGTKEFRNLVSEIAMLIVYE
                     ATRSLPLFDVKVKTPCGIANCKVISGRKLAFVPILRAGTGMLDGALALVPAAKVGHIG
                     LYRNEEDMKPVEYYCKLPADIEERDVFVMDPMLATGGSAINAISQIKKRNPKSIKFLC
                     IIAAPEGLKALTEYHKDVDVYCAGLDDHLNEHGYIVPGLGDAGDRIFGTK"
     gene            complement(15825..17609)
                     /locus_tag="PLD98_01570"
     CDS             complement(15825..17609)
                     /locus_tag="PLD98_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04972.1"
                     /translation="MKRKILSTLLAVLMLAALLPLGALADDTDAVAKIGDTYYNTLAE
                     ALAAVKNGGTITLLRDIDLDNQPWTPLSRSNVGFTFDGDGHTISNLKVNTTSNSAGLF
                     GSIGFSESTTTIRNLNLHNVDVRGNSYVGAVAGWITGTAVFENITVSGDVKVQANAQN
                     AGGILGHSAGVITVNNCHINATSGSYVKSNTTSGGGAGGIVGFAANEGSKACTISNCT
                     VKNLDVSGYHRIGGIVGMMQLVSGIYPSVHIYVTDNSVENVHVTVTGGSAATLNNRHD
                     AQVGLIVGATTDNGGRSRHHYYNNTYSGDCLVTATTGAIDIIPTALYGLWTLGAARVK
                     DYGQTNVGWYNTNGNADIIYADGAAGEVTFGGTEYITFLTAYRAGESSADNPFILHTD
                     VGYKLKPTDKIYVEANGNIFYPFADAGYEIQVTEISETVKLYELISVAEVKAEKIALL
                     NSYPSSEVIDDIIAQGIAVINAATTVEEVINAYNSTVQAIEAALQAAELAAAKAEAKA
                     RLVEAAGEDPSAAMQAIVDEYCARIDSAETISEVESIEEEGLQAIKAQYDKEHPIKEL
                     ITIEKIIRKFFGYIGKIIDFIKSFFTFT"
     gene            17988..18377
                     /locus_tag="PLD98_01575"
     CDS             17988..18377
                     /locus_tag="PLD98_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009532708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HQA04973.1"
                     /translation="MASVSSGILGDANLMNLKEMSVTQVRPEVNSLLVSDEEIIQCFK
                     TIRDQVIFTNKRVLVVNVQGETGKKVAYFSYPYSKVQYFGVETAGLLDIDSELILAFN
                     DGNRLQFDFKAQVDIARICSIISSFVL"
     gene            complement(18517..18592)
                     /locus_tag="PLD98_01580"
     tRNA            complement(18517..18592)
                     /locus_tag="PLD98_01580"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18557..18559),aa:Pro,seq:ggg)
     gene            complement(18767..19117)
                     /gene="ssrA"
                     /locus_tag="PLD98_01585"
     tmRNA           complement(18767..19117)
                     /gene="ssrA"
                     /locus_tag="PLD98_01585"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(19146..19610)
                     /gene="smpB"
                     /locus_tag="PLD98_01590"
     CDS             complement(19146..19610)
                     /gene="smpB"
                     /locus_tag="PLD98_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQA04974.1"
                     /translation="MSKEAIKTVVTNKKAFHDYFVEESYEAGIELFGTEVKSIRQGRV
                     NLKDAWCSIVNGEIFVNNMHISPYEHGNIFNRDPLRVKRLLMHKREITRLYGLTKQQG
                     YAIIPLSVYFKKGRVKVQVGLCKGKKLYDKRAVEAKKASEREIERAFKERRF"
     gene            complement(19632..21314)
                     /gene="argS"
                     /locus_tag="PLD98_01595"
     CDS             complement(19632..21314)
                     /gene="argS"
                     /locus_tag="PLD98_01595"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQA04975.1"
                     /translation="MSIVVKKAKNQIVEAVEKAFKTAIEKGLLPEGEHAPVIIEVPAD
                     SAHGDYSTNAAMASARVFRMAPVKIAEIVRDCIVTDGTYIASCETAGPGFLNFRLNSD
                     YYSDILKDALEKKEQYGHSDIGKGQSVLVEFVSANPTGPMHIGNARGGALGDVLSEIL
                     IWAGYRTEREFYVNDAGNQIEKFGLSLDIRYRQIFDPSIEMPEDAYLGQDIIDHAKAF
                     YEQYGDKYLNVPDEERKKALIDFALPKNIKALEEDLLKYRIKYDNWFRESTVHANGSV
                     QRVLKLLKESGYTYEKDGALWFMAEKFGCDKDFVLVRSNGIPTYIVPDIAYHYNKLVE
                     RGFDRAINILGADHHGYVPRLKAALQAIGIDPERLIVILMQMVRLVRGGEVVKASKRS
                     GKAITLVTLLEEVPIDAARFFFNLREANTHLDFDLDLAVENSSKNPVYYCQYAHARIC
                     SIIKRVSARLDEIKTEPKFELLDTPEERELIKHIASLTEEIEFAAKNLDPSRITKYAI
                     DLATAFHKFYNANRVDVEDKDLFKARLCLCIAVRDVMKNILNMMKVDAPEVM"
     gene            complement(21339..21758)
                     /locus_tag="PLD98_01600"
     CDS             complement(21339..21758)
                     /locus_tag="PLD98_01600"
                     /inference="COORDINATES: protein motif:HMM:NF020711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HQA04976.1"
                     /translation="MKKNVVIRITNIQSEGPQMVENHEVTTKGTFTGNENDYTLNYIE
                     LFDENTKSNTTIRVNKKTVTLIRQGDFNSEFIIEQNSRHNCHYETPYGEFMLGIYGKS
                     VESTVTENGGVLRLNYTLDFNAGFASENEMTIEFSVI"
     gene            complement(21797..22867)
                     /locus_tag="PLD98_01605"
     CDS             complement(21797..22867)
                     /locus_tag="PLD98_01605"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HQA04977.1"
                     /translation="MNNILVVFGGKSSEYEVSLRSSECIIRNIPRDKYNVYTLGITRD
                     GSWLYYDGSVDLIPNNEWISSGKTYPAVFSSEFGSRELLVFDNDGSVKEKIKIDAAFP
                     VLHGKNGEDGTIQGLFEIYGIPYVGCGVLSSAACMDKAFTNLVADAAGIPQAKWLSFN
                     SFEYSSNSEGIIADIIDKLGLPVFIKPANAGSSVGITKAKTREDLKKAIELALEHDCK
                     IVAEEAINARELECSVLGTMQEVRTSVVGEIRPQNEFYDYEAKYISDDSELIIPAPVS
                     EETSNELRRLAEKVFRTLDGRGLARVDFFLDKDSGRLLFNEINTIPGFTSISMYPKLF
                     EHAGLPCPELINELLKIALKGK"
     gene            23161..23859
                     /gene="sigK"
                     /locus_tag="PLD98_01610"
     CDS             23161..23859
                     /gene="sigK"
                     /locus_tag="PLD98_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HQA04978.1"
                     /translation="MLQALIDFIAANILYLTLHLNNYGAFPPPLKPSEEKMYFERLKN
                     GDEEARKILIKHNLRLVAHIIKKYYSGCAEQDDLISVGTIGLIKGINSFNPDRGIKLA
                     TYAARCIENEVFMYFRQERKGMQDISLSEPVETDGEGNPLTLMEIIKVDDTIVDDIDI
                     KLKEKMLKKFVEDIPNERDKTIIILRYGLNGGKPMTQKEVAKQLNISRSYVSRIEKRV
                     LKYLNRKFETETCI"
     gene            complement(23956..24084)
                     /locus_tag="PLD98_01615"
     CDS             complement(23956..24084)
                     /locus_tag="PLD98_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04979.1"
                     /translation="MKAFLERKSNILPTLIVLGVTLITIVIAVLFNEVSPDALLMF"
BASE COUNT         6558 a         5752 c         5589 g         6489 t
ORIGIN      
        1 acagagcttg aaggcaatat aatcgccggc gccaccgact ataacggcat tgactggttc
       61 attaccgaca gatatctcta tacaaactac gagcgcggca cattgtaccc ctatcacgag
      121 accgaaaccc ccgactgcaa aggtcttgcc gcatttaacg aggacttcgt cttcgcagca
      181 ggctcttttg gactgcagat aattcacgga aagcgccccc gctgggcaac gctcataacc
      241 caaacctcca aaatccccac taacgagctt acggctatcg cagcggacac atggaatcat
      301 gtctggatag gcaccaagaa cggcgctctt atctttgacg gcagaaacag atggctcact
      361 cccgacaccg ttaaggctct gcccagggca agcgttacca caatagccat cggcaaaaac
      421 ggagccgcat acatcggtac ggaaatcgga ctttatataa tcgacggcac aaaggagagc
      481 ttcctcggct ataaccgctg gctgcccgac tttaaggtaa cggcaattgc ggtaagctcc
      541 gacagcgaca ccctgtgcat aggcacagaa aagggtcttg ccatcatcga gtttaagctg
      601 atgtcccttt ccgaaaaggc cgagtactat cagtacaccg ttgaaaaata ccacaaccgt
      661 gagggctaca ccaccaaccg ccagcttacc gaaaagggca atatagagtc aggcgcaccg
      721 cagattaccg acaacgacgg tctctggacc ggctgctatg ttgcgtctca ggcactccgc
      781 tacggcgcca caaaggacaa gaaagccctt gaatccgcaa ggaagggcat gagagccatg
      841 ctcaagctca tgagcattac gggcatcgag ggctttaccg cgagagccta cagacgcccc
      901 ggcgagcccg gcttcggtga cggcgacaag gaatggcatc ttaccgagga cgagttcggt
      961 ccgcttgagt ggaagggcga aacatccagc gacgagatga cgggtcactt cttcggtctc
     1021 tccctctact acgacctctg cgccgatgag aaggagaaga aggaaatcgc ccttgccctt
     1081 tcaaatatta tggaccatat tctcagccat gaatacaccc tctgcgatgt tgacggtctg
     1141 cctaccacat gggcgcattg gggtccccat gagcttaaca acgacgagaa atggttctgg
     1201 gagaggggaa tcaactccct tgaactgctc gctttcctca aaatcgtcta tcacatgaca
     1261 ggcgacgaga agtatcagaa ggaatacgaa aagctcatca gaacgcagaa ctacgctctc
     1321 aactgcgttc aatacaagat tttcgacgca catacctccc acatcgacga caatctgggc
     1381 ttcctttgaa tgacatccct tctcagatac gagcaggatc ccaccatcag gcagtacttc
     1441 ctcatgggct ttaagcacca ttggaacgac cagcgcattg agcgctcacc gctgtggaac
     1501 attatgtacg gcgccctcac aggcgagcac tgcgaccttg aaaacgcggt cagaagcctt
     1561 gaggaattgc cccttgacct tatccaccat aaggttgtca acagcgtccg tcccgaaatc
     1621 gtctgggaca gcggtcagga actgttcggc ggcgtaaggc agctcaaaga gcctttgccc
     1681 tatgacgaaa agccgatgaa caagtatgac aacaacccct tccttgctga caggggcgac
     1741 ggtctcagag cagaggacgg caccgtattc ctgctgcctt actggtatgc ccgctactac
     1801 ggaatgatta tagaagagta ataaaatcca acgcgcgctc atatgagtgt gcgttggaaa
     1861 tgcttttgtt tagctgataa taatttaagt ttaacaatat aattaaaggg gtcttttaaa
     1921 tgagtttttc tgattatgcc aaaaatttcg gcggagttta ctccctgatg ctcacgcctt
     1981 ttaaagagga cagaagcata gactacgaca tctatcccga atatgtccgc tggcaggagg
     2041 agcagggcgc ccattatctc tttgcggtat gcggcagctc cgaaatgaag aatctaaccc
     2101 ttgaggaaag ggagaaatta gccgctctta ctgtaaagca ccgcaaaaag gtaaaggttt
     2161 tcgctaccgc caaccttgag ccttcatggc atggtcagct tgaagaaata aagagaatcg
     2221 aagctgcggg cgttgacggt cttgtatttg taaccaaagg ctacggcaat gacgaggaaa
     2281 ggcttgttac atatcttctg gagcttcaaa gccacgccac cgtgcccgta atcctttacg
     2341 agttccccgg tttctcaaac catctcatct caggcagcgc ctataagcgt cttgtggaaa
     2401 acggcaattt ccacgccatt aaggacacca cctgcacaat gcagggcatc aaagagaaaa
     2461 tagccgtaca gggcgactcc tgcgttcttc aggctaatat tccttacctt ctggaggcat
     2521 acgaggcagg cgcaagaggc gtcgccgcaa ctcccaccac ctgcggcaca aacctcttta
     2581 tcagaatgtg ggacagcttt gctgcgggag acatggaaga agcaagaaga atgcacgagg
     2641 aaatcgtaaa ccttgacaac gccctcggcg gagcattccc cgcaaccgcg aaatatcttg
     2701 taaatcttca gggaatcccg atgaactggt acaccagagg cgaccatgta ttaaaggacc
     2761 ataagctcaa agctctcaga gtctaccacg actacgccgt tagaaataat ttaatgaaat
     2821 aagagagaag gggtaaaagt ggagaaatat ctgattatca atgccgatga cttcggcatg
     2881 tgcagagccg caaaccttgc tacattcgac ctttttgaaa agggcggaat ctcgtccgcc
     2941 acaatcatga tgccctgccc gtgggctccc gaagctgtca agtgggcggc aagccacccc
     3001 gagtacgccg tgggcgtgca ccttaccttg acaagcgaat ggaagtacta cagatggggc
     3061 cccgtagccc aaagcggcac cgactcgctc agggacgaat acggctatat gtaccccgag
     3121 tgcgacgagt ttgaggaaaa ctgcgacatc gacgaggttg aaaccgaaat cagagctcag
     3181 gtcgcccttg ccaaaaggct tggacttaat ccttcccacc tggacaatca catgggttct
     3241 ctctacggct ttaacggcaa tatggaggtc atgcccagaa ctcttgaaat ctgcggagaa
     3301 ttaggttatg ccttccgtct tttcagaagt ctgcgccgcg agttcattcc ctccaccttc
     3361 ccgcaagacc ttcccttaga ccttcttaac atgcttgtag ccggctatgt ctcaattgcc
     3421 gatgagaagg gcgtccccct cattgacaat attctgttcc ccgagtggac ggaggacggc
     3481 aaagccgact acgaaaccta cagagagtac atgctaacaa ggcttgccaa catcccagaa
     3541 ggcgaaatta ccgaaacctt tatccatccg tctttggagt gcgacgagct taaaggcatc
     3601 acaagtcttt ggcactgcag agtttgggag cacagacttt tctcagatcc caaaacacgc
     3661 cagcacctcg aaagccacgg cgtaaagtac ataaactaca gagatgtggt aaggatgaga
     3721 aaagagcagg cttcaaaaga atagagatat taggtgcagt agctaagact actgcccttt
     3781 ctcttaaaaa ctgccaataa aagaagataa aagaaattaa gaattgatat tgacaatttt
     3841 agtttctgta tatataatac tttagcaagc taacgcaagg gccattagct cagttggtta
     3901 gagcaaccgg ctcataaccg gttggtccgg ggttcgagtc cctgatggcc caccagaatt
     3961 gccgaacttt tcattcgaag ggttcggaaa ttattagcct ttgcagcagc gaaggcagat
     4021 aggggtatag ctcagctggt agagcagcgg tctccaaaac cgcgtgccga gggttcgaat
     4081 ccttctgccc ctgccagata aaagccttga tttcctttat atgtaaggac ttcaaggctt
     4141 tttgctatat ttaagtcgtg accttcttct cactaaaaat taatgccctc cgtttgggag
     4201 ggtattttat tcttgctttc ttgtggcgaa gcattaagga gctgaaagca gcggctgcgt
     4261 tattaaagcg caagacctga gcaattcgtt gttttcgcta actttgctca atgtttgatg
     4321 gtatttacac acaaattata tttaaacaag ttttgcgaag aatatatccc cgccttttaa
     4381 agttacaaag aacttgccgt caatgcgttt aatctctttt gagccttcaa gcggcaggag
     4441 attcagcccg ctttttagtt ccaccttagc cgtgcgctca gcctcggggt tagccttctc
     4501 gccgtattcc acactttttt caatgccgtc attattaaat actgtcagat accagccgtt
     4561 cttgagcctg ttcacaaacc agtgcagacc tccggatacc ctgcagggca taagctcaga
     4621 tattttctcc accgcttcct tcgggctcat tatatttccg tgctttttct taaaagcagg
     4681 attaaagctc agatcaacaa ggtactcata ttctcctaaa acggattcgc agtcttcgtg
     4741 aataatatca aaagcgtcgg ggatatcgct gttcggcagc gttctatatt catttccgaa
     4801 tcccgaggca gactggccca taagctctct tattttctcg gcaatctttt tgtcccgctc
     4861 ttgagtcccc ttattctcat agggcatttc aagactttga ttaaacgaag aattgtaaac
     4921 atttgcaagc gccatcatat cttttggaag aactattgca atcggcgcgt aaaacttgcc
     4981 ttgatcgtat ttgcttataa agctcaaaaa ctccttttta acaagtccgt aagggcttag
     5041 ctcaaaattc tcatcgtcaa taaatgtatt atgcagtccc cattcctcac ttaaaaagga
     5101 agcgcccgca agatatgaat aaatgtatat acgcctttga agggagcggg aactgccgcc
     5161 ttcgccgcca agtgctttaa aggggaagtc cccctcagac tttatattcc actcattgat
     5221 tccgtcttta aaataggtgc agacagagaa aggcttgccg ccccacggct cataatatgc
     5281 tccgaaactg cccttatacg cctttgccat gccccttgca taggaaagct gtatgttggc
     5341 caaaggcgtc tgagcgccta tttcgggcat tactctcttt gcgcccagct taaattcaag
     5401 gttcgccgcc atcatagcac tgtcgcaggg gataagcctt ccgcccgttt cgttaagacg
     5461 cttgcggtac agcttttcgg catttattaa taattcctca aaagtctttg gtcttttaag
     5521 ctcaatcgcg aattcttcag ccgttgccgc ctcaaggaaa agatgcttaa agggaaactg
     5581 cctgaatatc tccttttcaa tcgtttcctt attccattcc tcgtccttga ccctgctcaa
     5641 cttattgtaa tctgtgcgaa ggttagacat ccattcgtgc atttgaaagc cgtaaaatct
     5701 gtcctctaaa gcctctatta attccatatc ataccgacag gtgtctatat atgcgccgcc
     5761 ttgaaggcgg tctatgtata caggaagcct ctcatcgtgc acaagagact tcagtttttt
     5821 gtcctccttc ataaggcggt taaacttttt ctcctcgggc agcattaaat tctgacaaaa
     5881 gcgaatgccc gcatttgagt taattatgcc cctttttaca aagcagtccc agagtttaga
     5941 gtcatagccg tgaaaaaaca caaaatcgct catatggcac catccttgta ttctttctgc
     6001 tatgctaaca cagcccaaag ttcaaatcaa ttgctcagtg taaatttaca atatagctgc
     6061 aaaatttaat ctttacagta tgacggtgat tttcactatt aaggatgcaa gagctttgaa
     6121 tatttttgtc ttgaacggca gccccaaggg tgagtacagc ataaccctgc agacggtgaa
     6181 ctacctgaaa ctatttttcc ccgagcaaaa ctttgaaaca tatcatgtgg ggcacaaaat
     6241 taaggcgctc gaaagagata tgagtcccgt tattgatttg attaacaaga cggaccttct
     6301 gttgttttcc taccctgtat atacctttat cgcgcccgcg cagctccaac gctttataga
     6361 gcttttaaaa cagtcggggt ttcccttgag ggcaaatacg ccgcccagat taccacctca
     6421 aagcgttttt atgatgtgac cgcccacagc tatatagagg aaaactgtca tgatttaaag
     6481 ctaaagtaca tagcgggtct gtctgccgat atggacgacc tgctaagcgt taacgggcaa
     6541 aaagacgcaa gggagttctt tgaatatgtc ctttggtgcg ctgaaaacga ttattatgta
     6601 aaatcgccct cagtgtcttg cagctacacg cccgcccctg gtactgtacc cgatgaccct
     6661 tcagagccca aaacgggcga tgtggttatt gtaaccgact gtgaggagaa cgacactcag
     6721 ctcaaaaata tgattgcccg cttcagagca gtattgccca ggaaaacaag ggtagttaat
     6781 atcagagagt accccttcaa aggcggctgc cttggctgct ttaactgcgc ggtgtcgggc
     6841 aagtgcgtct acaaggacgg ctttgacgac tttttaagaa acgagattca gtcggcagag
     6901 gcaattgtga ttgcatttac tataaaagac cacttaatgg gctcttgttt caagatgtat
     6961 gacgacaggc agttttgcaa cggtcaccga accgtcacaa agggtatgcc catgggctac
     7021 cttgtcagcg gcgcgttgga agcagagcag aaccttaaaa ccataatcga ggccagagcc
     7081 cgggtcggcg gcaacttcct tgcgggcgtc gcatcggatg agtctgaccc cgactctgaa
     7141 attgataagc tggcaaacac tctggagtac gctttgcagc acaaatacgc cccgcccgga
     7201 aatttctacg gcgttggggg tcttaaaatc ttccgcgacc ttatctggct tatgcagggc
     7261 atgatgagag ccgaccataa gttctacaaa aagcacggaa tgtacgactt tccccagaaa
     7321 aagaagggca ctattctcaa aatgtacgcc gtcggcatgc ttttagcggc gcccaaagtc
     7381 aaagccaaaa tgggcaatat gctaaacgaa ggaatgctga tgccttataa aaaacttctt
     7441 gaaaaacaaa atcaaactta tcttctcaat aaggcaatcc tatctttttc acaataaatt
     7501 ttccctttca tataatggta tagaacaata tatatgaaag ggatgaacta agtgcattac
     7561 ggcaattttt caggacaaaa catcagagac tacggtcccg agccgctggt agttaatata
     7621 gacagattcg caaagatgaa tcaaaattac agaactgccc tgtggacggg aaaacatctt
     7681 caggtaacac ttatgagtat tagggtcgga ggagacatag gcctcgagat gcacccgaat
     7741 gtcgaccagt ttatcagaat cgaaagcggc tatgcccttg tcaagatggg caactgcaag
     7801 gacaatctga actatcagag aaagattgac agcaactatg cggttatcat tcccgccgga
     7861 acatggcaca acattatcaa tatcggcaaa actccgctca agctctattc aatctacgcc
     7921 ccgcctgagc acccccgcgg aactgttcac agaaccaagg aagaagcaat gcgggcagaa
     7981 cagcattaat aatccctggg gcaaagaaaa cggcaaaact tcggttttgc cgtttttttt
     8041 ctttggaatt cattattttt tgtgaaattg gataaaaaag atttgaaatc aaatcaacat
     8101 tttgttaaac ttagtctatg aattaaatat gtatttagta ataacaaaca gacatactgc
     8161 cgcaggatgg gggcgattaa ttggtaatag agcattttgt agagaacgaa aaggaagggc
     8221 agtattatac catccccttc agcgtaccgc agaatgtaat aaagattacg gttaaatatg
     8281 agtattacag accgacaaag ggtattctct ccgaccttag acccacaaac tgcattgaca
     8341 tcggtcttat gaacgaaaag ggcagattcc tcggctggtc cggcagctcc cattccgaaa
     8401 tcagcgtagg ggaatacagc tcaaccgacg gctacataac gggaaaaata aacccgggtg
     8461 agtggaggat aatcatcggc gcctatcatg ttgagaaaaa gggcgtaacc gtaaagtaca
     8521 ccattgactt tgagttcgcg ggcgaaaagc tcctattcgg cgaccttcac attcatacaa
     8581 aagcctccga cggcattttt gatgtctctc aaatagcgaa tatggcaagg gaaaaggggc
     8641 tcgatttcat cgctgtcgca aatcataaca actatgccga gaactttgcc ctccccgctg
     8701 taaacggctt aacctacatc cccgccgttg aatggaccca ttacaagggt catatgaatt
     8761 tctatggggt taaaaatcct tttgaggatt cctttatcgc aaacagcata gaagaaatgc
     8821 gaaaggtaat agaaaaggca aaagccctcg gagcggtcat ttccgtaaac caccccaaat
     8881 gcgccattat cccttatttg tgggaggacg attatatttt cgatatggcg gaaatatgga
     8941 acggtcccat gcgccccaca aatgtaagag gcatagccta ctggacctcg cttttaaagg
     9001 cgggcaggaa ggttgtggcg gtgggaggca gcgactatca cagacccctg cgctttgtca
     9061 aaatcggcaa ccccgtaacc ggcgtgtatt ccccctccca aagcgctgag gatatactcg
     9121 attccatcaa aaagggacgc tgctttgttt ccgagtcaaa agacggtccc gccatagacc
     9181 ttaaatacgg cggctttaga atgggcgaca ccgctgaata taaggaaggc accgatttaa
     9241 cggtagaagt taaaagccga tacaaagtta aactatatct cgttacggac aagtacgaaa
     9301 aggaaataaa agctggcaaa gtcccgctcg ataaggtgaa attcgcgtat atcaaggcaa
     9361 caaaaaagca cggcaataaa gttcgcgcgg tttcaaaccc gatttatttt aactgacagg
     9421 aggaaatcca atggcagcag aatcaaggct agcaaaaaga accagcccgt caagatacgg
     9481 cttcgcgatg ttcggaacat caataccaat caatatgttc aaatccttcg cggcgattta
     9541 ctatgtcgat atgctgggac ttgacatgaa aaaatactcc cttgtgcttt tcatctatac
     9601 ctttgtggac gccatcgaca accccgttta cggcttcttg tccgacagaa caaggacaaa
     9661 atggggcaga agacgcccgt ggcttttaat cggcacgccg cttctggttt tgtcctttat
     9721 ccttttctac aatgtgcccg cctttgtgca aaccgaaaag ggcatgctct ttatatacat
     9781 gcttctcatg tatatactca cgggaaccct tgactcactt gtcaacgcaa actacggagc
     9841 ccttttcccc gagcttttca aagacgatgt ccaaagggca aaggtaaacg gcttccgtca
     9901 gatatgccag cttttcgcca tggcgataag cattgccctt actcccatga ttactggcgc
     9961 aatcggctac agacttactt ctttaatcta cggtattctc gctttagccg taatgatgta
    10021 ctgcttcttc ggctgccatg aggatttgga gtacatagaa gatttggaaa agccgagcct
    10081 tttcagcagc gttttcgcac tgatttcaaa tcctaagttc tggattttcg gctttgtggg
    10141 cgcattctat tccgcggctt tctcactcat ttctcaggct gtttcgttct atgtaaagta
    10201 cactctgggg ctcggaggca atgtcaccac cataatgctt ggcgtggtgc ttgtggttgc
    10261 ggtttcgggc atagttttct ggtcttggat attaaagaaa tggaccgtca tgaaggtctg
    10321 gagaacagga cttattataa tggctgtcag cttcctgccc ctctttttca tcaacagcct
    10381 tataccagca ataatagccg cctgctttgt gggctttgga gtcggcgcct gccttaccac
    10441 catggactgc atcggcgcga aaatcgttga cgacgactac gcaaagcacg gcataaagcg
    10501 tgaaggcatt ataaactccc ttatgggcgt tatgaaccgc ctttacggac tttatacttc
    10561 ccttgccttc tatatcgttt ccgtcaccta cgattttgta aacggcgaaa atcccggacc
    10621 caatcccgct atggcgtcaa gagttctttt gtgcttattc cccgccgcgg cgatggtact
    10681 tgcgtcgatt atcagcttct tcttaaaatt ccccgagatg gacaaaaggg ataagagctg
    10741 aaggtgcctt ttatgagcag gtttcttata attaacgccg atgatttcgg ctgctgcaag
    10801 gagcagaacg aggcgataaa agagctgtgg gaaaataatc tcataacttc cgtctccgtt
    10861 ctcgcggtcg cgcccgaatt tgaggacgcg gctaattggc tgaaaagcaa agggctgtca
    10921 gccggcgttc acctgacgct aaactccgac gaggcgttaa atccttggag gagtattacg
    10981 ggcgcaaaat cccttggaaa aaacggcaat ctgcactcta attcaaagga tataaccttt
    11041 cacgcaagac gcagagatgt aagcagagaa attgccgccc agtacaatgc cattaaaaat
    11101 gcgggcatta aagtcgacca tgcggacagc cacagcggca cattgtacgg gctgaattta
    11161 agacgctttt acattgacgc tttccgcttc tgcggcgaaa acggccttcc ctacagattc
    11221 cccaaaagag cggattttat agaaagaatg cttggcagaa aaccgccagc ggctctttat
    11281 aaattccatt cccaccttgt aaacttggct aagaaaaacg gcgtgtgtct catcgacgat
    11341 atgctctcta acccatggag cattgacaaa attaaagata aagactcgat tttggagtat
    11401 tatctgaacg cggtaaaggg aataggggag ggaataacgg agatttttct ccaccccgca
    11461 aagccaaaga gcgaagagaa aacgccatgg accaaaagag tctatgagta cgagatactc
    11521 aaaagcggag cgcttttgga ggccgcaaag aaaaacggca tagaaatcat ctcctgggct
    11581 gatgtcaggg agattaataa ttataggagg accgaatgag aaagtttgta agtatagttt
    11641 tggctatagt actaatgttt tctgtttgca tgcccgcttt tgcggcaggc agtgtgggag
    11701 cggaaaatga gccaaccccg cccgaatacc ccctaatagt cattcgaggg gtaaacttca
    11761 tgaatgaata tcttgatgtg ggcacaaaga acgagagaat ctacataccg ccaattaaag
    11821 catcggatat attcgcggct gtgttcaagg gtgccgcggc ttatgcggtc acttcagaca
    11881 aaaagaaatt ccttactccc gttctcgact atctcaatga aacctttaaa tatatagcct
    11941 gcgacaaaaa cggcgactct gtctacaatg tgggcgttta cgaatacccc ttgtccgccg
    12001 cgaattacga ggatttcccc ttgggagatg accacgagga gggtattctc accgaagctg
    12061 taaaccgcta cggcgcggaa tatgtgtact atttcaacta cgactggaga atagaccccc
    12121 tggacgcagc ggacaaaata aacgaaatga ttgagcgcgc cctgctggac cacggctgcg
    12181 acaaagtgaa catcatctgc tgctccatgg gcggcgttat gacagtgggc tatctgtata
    12241 agtacggata cgacaaggtc aacaagattg ttttcctctc cgcagtttta gacggcacct
    12301 atatggtctc cgatgtatat cagggcaaaa ttaatttcaa tcccgtctat gcgtacaatt
    12361 tcctcagaga aggtctggga ctgcctttca tcgcaaaaat actcgttgat atcctttaca
    12421 aagcaggcgt catcgacagg ctttgcgaac ttctcaacag ctttgtggcg gaggaaaccc
    12481 agtttgtcct tgattacaca gtaagggata ttttcggcac cgtatcttcc tggtgggcgc
    12541 ttgtcataaa ggaagagatt gacgacgctg taaactatat gttcccgacc gatgaaatga
    12601 aagaggagta cagcggtctg cttcaaagaa tcgagcgtat caaggaaatg ctgtacgcaa
    12661 aggatgagct tttgcggaac gcaaaggcgg acggcgtggg aattttcgtg ctcgccagct
    12721 acgacaagcc gctctttccc ttctatgagc gcagcggaag cataggcgac ggtcttgaaa
    12781 ccgcccgtat ggcaggcggc gctataaccg cggagtacgg caagactctt gatgtggagg
    12841 aatccgaatt tctgtcaccc gacaagatgg ttgatgtaag cactgtttta ttccccgact
    12901 ccacatggat tatcaagaac gctccccatg tagcctgcaa ggtgggcagc gatttcgccg
    12961 aattcatgtt ctgccttatc gatacagagg agaatatgac agtctactca aatccccgtt
    13021 atccccgttt cctctgcgta gataaaaacc agaatttcgt gccttacaaa tagtctaagg
    13081 caacaaaaaa gaggctgcga taagcagcct ctaattttta tatttctatg attattcagc
    13141 ctttttcttc tctccgatgc tgagaattgc gttgaggaag atgccgaata ttgcggcgcc
    13201 tgcgatgcag ggaatgttaa gaccgaagaa cgggatgttt ccgccgtata catactgacc
    13261 gccgatgccg atgattacga tggaagcgat aacagcgata ttcttagagg agaacatatc
    13321 tgcgttcttg tctatcatga tagccacgcc ctgtgcggcg atagcgccga agaggtatat
    13381 ctcaagtccg ccgataactg cgaggggaat tgcgtaaata gcctggataa gaggtgtgaa
    13441 gcaggagatt atcatagcga taacagctgc gaccatcaga acgggaactg agaaaacctt
    13501 tgtgatagcc attgtgctga tgttctcgcc gtagttggtg cctgcgggac cgccgatgaa
    13561 accggtaatc atgtcgccga taccgtcgcc gataaggttc atgtggagac gctcaataag
    13621 gttatatttc tttgcgccct tcttttcagc aacatcgttt acatatatgt caagctgata
    13681 catatgtgct gtggattcgg ggattgttgc aattgctatg ggcatgattg ctgcgatgga
    13741 agccaaagct atcttgctgt tgctgaaatc ggggagcgag aatttcggga ttgcgaaggg
    13801 gcctgcggac cagagggact caatagcgtc tgcggagatg acgcggaaca tattgtaatc
    13861 tgcgccgcca atccagaaga gtatagccgc ggcaaggcag ccggttgcag ctccgagcag
    13921 aagcggaagc tgtccgagga agccctttaa gtaacgggaa tacaaaattg tggaaagcag
    13981 tgtgaccagg gatatgaccc aaacccaatt ggaagcgaat acgccggttg cgttggcgtt
    14041 gggagcggcg tctgccaacg cgttgcctgc aagggtcaga ccgataatca ttgcgacagg
    14101 gcctgtaatg gtgggcggaa ggattttttc aactgccttg atgccgacga atttaacaag
    14161 aagtccggcg gcgatggaaa caagaccgga cattacaata ccgacctgtg cataccggat
    14221 ggttgcggtg ggcattgtaa agaaacctgc ctcgccgtta aggaagccgt tgagctgtgc
    14281 gatttcatct gctgaaagcg cattggtaac cgtttcactt gacataagac ctataactgc
    14341 cgataaataa gcaaagctgg agccgtagta aagggggatt ctcctgcctg taatcaggac
    14401 aaagcaaagg gttgccaatc cgctggcgaa aatcgtggtc gatacatgga agcctgtaat
    14461 cagcgcaaca gtgattgttg cggggaacat gactatgacc tgctgaagcg cgtaaagcag
    14521 cagtttgccg actggcggct tttcatccgg taaatatccg tgtattcttt cttttgccat
    14581 actgttctct cctcaatttt gttttttact gtttttgttt tcacgctcca aacttaaggc
    14641 tgcctttggg cttgtcctgc ggagagcgaa aacactttag aactcgcgca cccgggacgg
    14701 tctttggctc aaagccccct gcgaccgcac aatgccgact cccggcaagg aaaggggtaa
    14761 atctgctccg agttttgctc gttctgtaaa cgcggctttt tctcctgtta ttcctccttt
    14821 gggtccgaat ttggaaaatg tattccaaat tttataaatt tttacaaaaa aaatcagttc
    14881 cttttgagaa ctgaattttt aaactatttg gtgccgaata tcctatcacc cgcgtcgcca
    14941 agaccgggta cgatgtagcc gtgttcatta agatgatcat caaggccggc gcaataaacg
    15001 tcgacatcct tatgatattc ggttaaagcc tttaagccct caggggcggc aattatgcaa
    15061 aggaacttaa tgctcttggg atttctcttc tttatctgag aaatggcgtt tattgcggaa
    15121 ccgcctgtcg caagcatggg gtccattaca aatacatcgc gctcctcaat gtcggcgggg
    15181 agcttgcagt aatactcaac gggcttcata tcctcttcat ttctgtaaag accgatatga
    15241 ccgacctttg cggcgggaac aagagcaaga gctccgtcaa gcatgcctgt accggcacga
    15301 agaatgggaa caaatgcgag ctttctgcct gaaataactt tgcagttggc tattccgcag
    15361 ggagtcttaa ccttaacatc gaaaaggggc agcgagcgcg ttgcctcata aactatcagc
    15421 attgcaattt cgctgacaag attacggaat tctttggttc ccgtgttttc atccctcaag
    15481 aatctcagct tgtgctgaat gagaggatgg tcaagaataa cgacttgtct ttccatggtt
    15541 ttcgcccctt cgtcaggtat catcaatttt tgcacttggt tgatagtagc ataatttgtt
    15601 ttctatgaca agaggatttt ttgaattgta aaaatttttt cacaacatat tgtttctcct
    15661 cattttcaga gaacaatttg tagagaataa cgaaatttgt cccgtcatga ggtgctttaa
    15721 aaaagttatg ctttattatg ttttccaaga ttggaatata aatgcccgag ccgcattttt
    15781 acagctcggg catggtggga taatatgggg acggagactt ccgattaagt aaatgtgaag
    15841 aaggacttga tgaaatcgat aatctttccg atgtagccga agaatttgcg tatgattttt
    15901 tcgatggtta taagctcttt tatgggatgt tctttatcat actgagcctt gattgcctgc
    15961 aggccttctt cctcaatgct ctctacctcg ctgatagttt ctgcgctgtc aattcttgcg
    16021 cagtattcgt caacgatagc ctgcattgct gctgagggat cttcgccggc agcctcaacg
    16081 agccttgcct tagcctcagc ctttgccgct gccagctctg ctgcctgcag tgctgcctca
    16141 atggcttgta cggtgctgtt gtatgcgttt ataacttcct caacggttgt tgcggcattg
    16201 ataactgcga tgccctgggc gattatatcg tcgattacct ctgaggaggg atagctgtta
    16261 agaagcgcaa tcttctctgc ctttacttcc gcaacggaaa tcagttcgta aagctttacg
    16321 gtttcgctga tttcggttac ctgaatttca tatcctgcgt cagcaaaggg atagaagatg
    16381 tttccgtttg cctcaacata aatcttgtcc gtgggcttga gcttatatcc aacatccgta
    16441 tgaaggataa agggattatc tgcggaggat tcgcctgcac ggtatgccgt aaggaatgta
    16501 atatactcgg ttccgccgaa tgttacttct ccggctgcgc cgtcagcata tataatatct
    16561 gcgttaccgt ttgtgttgta ccagcctaca tttgtctgtc cgtaatcctt aactctggcg
    16621 gcgccaagag tccaaagtcc gtacagcgct gtgggaataa tatcaatagc accggtggtg
    16681 gcagttacta agcaatcacc cgaataggtg ttgttgtaat aatgatgcct tgagcggccg
    16741 ccgttatctg ttgttgcgcc tacgataaga ccgacctgtg cgtcgtgcct gttgttgagt
    16801 gttgcggcag aaccgccggt tacagttaca tgtacatttt caacagagtt gtcggttaca
    16861 taaatatgaa cgctgggata tataccggat acaagctgca tcatgcctac aatgccgccg
    16921 attctgtgat agccgctgac atcaagattc tttacggtgc agtttgaaat ggtgcatgcc
    16981 ttgctgcctt cgtttgccgc aaagccgact atgccacctg cgcctccgcc gcttgtggtg
    17041 ttcgacttaa cataagaacc gcttgtggcg tttatatggc aattgtttac cgtaattacg
    17101 cctgcgctgt gacctaagat tccgccggca ttctgagcgt ttgcctgaac cttaacatcg
    17161 ccgcttacgg taatgttttc aaacactgcg gtaccggtaa tccagcctgc aaccgcgcca
    17221 acataggaat tgccccttac atccacatta tgaaggttga ggttgcggat ggtagtggtg
    17281 gattctgaga atccgattga gccgaaaagt ccggcactgt tcgatgttgt gttaaccttc
    17341 agattgctga tggtgtgacc atcgccatca aatgtaaagc ctacatttga tctgcttaaa
    17401 ggcgtccaag gctgattgtc aaggtcaatg tcgcgcaata aagttattgt tccgccgttc
    17461 tttactgccg cgagtgcctc tgcgagagta ttgtagtaag tatcgccaat cttagcgact
    17521 gcgtcggtat catctgctaa tgcgcccaag ggcagaaggg cagcaagcat cagaaccgcc
    17581 agaagcgtac ttaaaatttt tcttttcatg cgcattttcc tttcttccgt cagttttcgt
    17641 tgtgcggcta gagtataact aatgtaattt aacctaatat tggaaacttt gcaacgataa
    17701 aatacaaaaa tagacaaatt atgctgtcaa aatcgcatct tttttgcgac tttgctgaaa
    17761 acgcataaat attagccttt taatggaaaa aatgtaatat ttggactcca tatcgacaaa
    17821 attcgataat aaattcgccc cttcgccgag tgtcctcagc aaggcatttt tgttttcagc
    17881 tttagctaag tcccaataat cgtgcacttc ttttaggtat acttctattg atttttggaa
    17941 aaatgaagat ataataaaac taattttttt atgaggggga ttttaaaatg gcaagcgtat
    18001 cttccggcat tttgggcgac gctaatctaa tgaatttaaa agaaatgtcg gttacacaag
    18061 ttcgtcccga agtgaattct ttgcttgttt cggacgagga gattatccag tgtttcaaaa
    18121 ccatccgtga ccaggttatc ttcacaaaca agcgtgtttt ggttgtaaat gttcagggtg
    18181 agacaggcaa aaaagtagcg tatttttcat atccttattc aaaggtgcag tatttcggcg
    18241 ttgaaacagc gggtcttttg gatattgaca gcgagctgat acttgcattt aatgacggaa
    18301 accgcctgca gtttgatttc aaagctcagg tagacattgc gcgcatttgt tcgattattt
    18361 cttcctttgt tctgtaaagc ggcaatttcc atagggcatt aaaataatta aaacccgtcc
    18421 cgaaagcatc tcgggtcggg ttttttaatt cctttaaaca atggatttta acataaaaaa
    18481 gacagcagat tttctatcaa aacctgatgc cttttgtggt ccgagtggcg ggacttgaac
    18541 ccacggcctc ttgaccccca gtcaagcgcg ctcccatctg cgccacaccc ggatattatt
    18601 attttatatt tttaaggctt tcttgtgcat ccgagagcct attatatagc acttttttct
    18661 ctgtgtcaag ctgtttttaa ttatatttgt tttatttatt ttgcaaatta gaaggggagg
    18721 ggggagaaaa cataacgccg gcttttattt aagccggcgg atactttggt ggagatggcg
    18781 gtaatcgaaa ccgcgtccga aaatccattc ccgcaacttt ctacgagcgt agccgtttat
    18841 ttaaacttcc cctcgaccga cgccaaacgg caggcttcgg tgtcgggtag ttccattatt
    18901 catgacggac tatggaacac tcgtccgttc acgttcaccg ctagtcgacg cctttatccg
    18961 aaccgcggta gtttcggtaa agacggctgc ttatacttta agcagctgct aaaactctat
    19021 tgttctcgtt tatttttaaa ctgcggattt tatagcggtc ccgcaccgct gctcgcttat
    19081 cacggttcaa gatccccgtc gaaatccttt catccccatt tttaagcaga tgaaagagtt
    19141 tagtcttaaa accgtctttc cttaaaggcg cgttcgattt ctcgttcaga tgctttttta
    19201 gcttcaacgg ctcttttgtc gtaaagtttt ttacctttac agagcccgac ctgaacttta
    19261 acgcgtcctt ttttgaagta taccgacaat ggtattattg cataaccctg ctgttttgtc
    19321 aatccgtaaa gcctggtaat ctctctctta tgcataagca ggcgcttgac cctaaggggg
    19381 tccctgttaa atatgtttcc atgctcatag ggcgaaatat gcatattatt tacaaaaatt
    19441 tcaccgttta ctattgagca ccaggcgtct ttgagattta cccttccttg ccttatggat
    19501 ttgacctcgg tgccgaaaag ctcgatgccg gcctcgtagc tttcctcaac aaaataatcg
    19561 tgaaaggctt ttttgtttgt aacaaccgtt ttgatagctt ccttactcat gttgccctcc
    19621 tttctttgag gctacataac ctccggagcg tcaaccttca tcatattaag aatatttttc
    19681 ataacatccc tgaccgcaat gcaaaggcag agccttgcct tgaacaaatc cttatcctcc
    19741 acatcaaccc tgttggcgtt gtagaacttg tggaaggcgg tagcaaggtc gattgcgtat
    19801 tttgtaatcc tcgacgggtc aagattcttt gcggcaaact caatttcttc ggtgagagac
    19861 gcgatgtgct tgataagctc cctttcctcg ggagtatcga gaagctcgaa cttaggctcg
    19921 gtctttattt catcaagtct tgcgctgacc ctcttaataa tgctgcaaat cctcgcgtgg
    19981 gcatactggc agtagtaaac ggggttcttt gaggagttct cgacagccag gtcaagatca
    20041 aaatcaaggt gagtgttagc ttctctcaga ttgaagaaga acctcgctgc gtcaatcgga
    20101 acctcctcaa ggagagtcac tagggtaata gccttgccgg agcgctttga tgccttgaca
    20161 acctcgccgc ctctgacaag acgcaccatc tgcataagaa ttacaatgag cctttcgggg
    20221 tcaattccga ttgcctgcaa tgcggctttg agcctcggca catagccgtg atggtccgcg
    20281 ccgagaatgt ttatggccct gtcgaagcct ctttcgacca gcttattata atgatatgca
    20341 atatccggaa caatgtaagt gggaattccg tttgacctga caagcacaaa atccttgtcg
    20401 cagccgaatt tttctgccat gaaccaaagc gcgccgtcct tttcgtaggt gtagccgctt
    20461 tcctttaaaa gcttaagaac ccgctgaacc gaaccgttag cgtgaacggt gctctcgcgg
    20521 aaccagttgt cgtatttgat gcggtatttc aaaaggtcct cttcaagggc tttaatgttc
    20581 ttgggcagag caaagtcgat aagagccttt tttctctcct cgtcgggtac attaagatac
    20641 ttgtccccgt actgctcata gaaagccttt gcgtggtcga tgatgtcttg tccgagataa
    20701 gcgtcctcgg gcatctcaat cgacgggtca aaaatctgcc tatatctaat atcaagggag
    20761 agaccgaact tctctatctg attgccggcg tcgtttacat agaactccct ttcagtgcgg
    20821 tagcccgccc agataagaat ctccgacaga acatcgccca gagcgccgcc gcgggcgttg
    20881 ccaatgtgca tgggaccggt ggggttggcg gatacgaact ccaccagcac gctttggccc
    20941 ttgcctatgt cggaatgtcc gtactgctcc tttttctcaa gggcgtcctt caatatatcg
    21001 gaatagtaat cggaattgag cctgaagttt aaaaagcctg gacccgctgt ttcgcagctt
    21061 gcgatgtatg ttccgtctgt gacaatgcaa tccctgacaa tctccgcaat cttaacgggc
    21121 gccattctga aaaccctggc ggacgccatg gcggcgttgg tggaataatc gccatgggcg
    21181 ctgtcggcgg gaacctcgat aattacgggg gcgtgctcgc cctcgggaag caagcccttt
    21241 tcaatagcgg ttttaaaagc cttttctaca gcctctacaa tctgattctt agccttttta
    21301 acaactatgc tcatctttca aattcctttc ggataatgct aaattacaga gaattcaatg
    21361 gtcatctcgt tttcggaggc aaagcccgcg ttaaagtcaa gggtgtagtt aagtctcagg
    21421 acgccgccgt tttctgtgac ggttgactca acggacttgc cgtaaatgcc aagcataaac
    21481 tcgccgtatg gcgtttcata atggcagttg tgcctgctgt tttgctcaat gatgaattca
    21541 gagttaaagt cgccctgtct tatgagggtg acggtttttt tatttactct tatcgtggtg
    21601 ttgctttttg tgttctcgtc aaatagctca atgtaattta gtgtgtagtc gttttcgttt
    21661 cctgtgaagg tgccctttgt ggtgacctcg tgattttcca ccatctgcgg accttcgctt
    21721 tgtatattgg taatcctgat tacaacattc ttcttcattt atatacttta cctaatcctc
    21781 tgctttctga tttcagctat ttgcctttaa gggcgatttt taaaagctcg ttgatgagct
    21841 cggggcaagg aagccccgca tgctcaaaga gcttgggata catgctgatt gatgtaaatc
    21901 ccggtatcgt gttgatttcg ttgaaaagga gcctgccgct gtccttgtca aggaagaagt
    21961 ctacccttgc aaggcctcgt ccgtcaagag ttctgaaaac cttttctgca agccgtctta
    22021 attcattgga ggtttcctcg ctgacgggag cggggatgat tagttcgctg tcgtcgctta
    22081 tatactttgc ctcgtaatcg taaaactcgt tttggggtct gatttcaccg accacggagg
    22141 ttcttacctc ctgcatggtg ccgagtaccg agcactcaag ctcccttgcg tttatagcct
    22201 cctcggcgac tatcttgcag tcgtgctcca aagccagctc tatcgccttc tttaaatcct
    22261 ctcttgtttt agctttggtg atacccaccg atgagccggc attggcgggc tttatgaata
    22321 cgggaagacc gagtttatca ataatatcag cgattattcc ctcggaattt gacgaatact
    22381 cgaagctgtt aaacgacagc cattttgcct ggggaattcc cgcggcgtcg gcaaccagat
    22441 tggtaaaagc cttatccatg catgccgcgc ttgacagaac gccgcagccc acatagggta
    22501 tgccgtatat ctcaaaaagt ccctgaatcg tgccgtcctc tccgtttttg ccgtgcaata
    22561 cggggaacgc ggcgtcaatt ttgattttct ccttaacaga gccgtcatta tcgaaaacca
    22621 aaagctccct tgagccgaac tcggaggaga aaaccgcggg ataggttttg cccgatgaaa
    22681 tccattcgtt attcgggatt aaatccacgc tgccgtcata gtacagccag ctaccgtccc
    22741 tggtgattcc caaggtatat acattgtatt tatcccttgg aatgttcctt attatacatt
    22801 ccgaggagcg aagcgacact tcatattcgc ttgacttgcc gccgaagact acaagaatat
    22861 tattcaaata aaacatctcc ctggagattg accgttttgc ggccatagca taatcattaa
    22921 taaatataat cgcgtaaatt aaataatagc acaattaagg cgaggtttgc aatcgaattt
    22981 atgcattaac ttccattttc gctcgacagc tcgttttgag ggaattcgac aaaacaagac
    23041 aggacctgca cttgcgcaca acggggagca tataatgtat tacctgtcat tatacacaaa
    23101 tttgggcgga aagagaattc tgttttaagc ggagtacttt tttcgttacg gggggctatt
    23161 atgctgcaag cacttattga ttttattgcc gcgaatattc tgtacttaac acttcaccta
    23221 aataactacg gagcctttcc gccgccgctc aaaccctcgg aagagaaaat gtactttgaa
    23281 cggctgaaaa acggcgacga ggaagcgaga aaaatcctga taaagcacaa tctaaggctc
    23341 gttgcccata ttataaaaaa atactattcc ggctgcgctg agcaggatga cctgatatcc
    23401 gtcggcacca ttggtctgat taaaggaatt aacagcttta atcccgacag aggcatcaag
    23461 ctcgctacat acgccgcaag atgcatagaa aacgaggttt ttatgtactt ccgtcaggaa
    23521 agaaagggca tgcaggatat ttccctgtcg gagcctgttg aaaccgacgg cgagggcaat
    23581 ccgctgacgc ttatggaaat tatcaaagtt gacgacacga ttgtcgatga catagatatt
    23641 aaactcaaag agaaaatgct taaaaaattc gtggaggata taccaaacga aagggataag
    23701 acaattatca ttttaagata cgggcttaac ggcggcaaac ccatgaccca aaaggaagtc
    23761 gccaagcagc tgaatatttc gaggtcctat gtttcaagaa ttgaaaaaag agtcctgaaa
    23821 tacctgaaca gaaaattcga gaccgaaacc tgcatttaaa tataaggcga atcgggctgt
    23881 gcaatgctgc acagcccgat tttttaaagg atgtataaaa gagacggaaa agaaggacgc
    23941 aaaatatagt acaagctaaa acataagaag ggcgtcgggg ctgacttcgt taaaaagtac
    24001 agcgattacg atagttataa gggtaacccc caaaactata agcgtgggaa gtatgtttga
    24061 ctttctctct aaaaatgctt tcatcgctgt atccctcctt gttgtttgca agttaattat
    24121 agaatcaatt tccgccaaag tacactaaag caaatttaca aaatgactac agatttgtaa
    24181 tctttacagg caatttatta caactctgaa atatgtgact tttttagaca aaattcaaag
    24241 aatccgttac tttaaaggcc tttgtataaa taatgcgaaa aaatgcataa atttaaaaaa
    24301 atatttgatt ttttggcttt ttgctagtat aatatggtat gatttattta aagtcagtgt
    24361 ataaatgcac tgaatatatt catcaaag
//
LOCUS       DAONVQ010000011        23493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_1512_length_23493_cov_5.111742, whole genome shotgun sequence.
ACCESSION   DAONVQ010000011 DAONVQ010000000
VERSION     DAONVQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 23493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23493
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1512_length_23493_cov_5.111742"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..3179
                     /locus_tag="PLD98_01620"
     CDS             177..3179
                     /locus_tag="PLD98_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQA04980.1"
                     /translation="MKYEINKTNHCTFPLFEKNKLSPRAYFIPFKDRKLLEKTDIRKE
                     RYSGDMVKVLSGEWDFKFYPKKSKMPSSINTSKIKFDKVQVPSTWQRTGYMDPVYLNV
                     AYEFTAVPPAIPEDMPVGLYRKLIDIEDLSKNYIITFLGVASSLDLYVNGSFVGYSEG
                     SHNSAEFDITKFLKKGENEIVAVVFKWSTGTYLECQDMFRENGIFRDVLLTEYEPVFI
                     YDYCLKTAKQGELYNLTAEIDIKGQPEGYDVEIEIVKDGAVIASAAADAKEQTVIKFQ
                     NLDVKEWNPEIPELYDVFITLKQSGREVMSIRNKTGFRTIEIDGEVFLFNGRKIKMKG
                     VNHHDTHPVKGYAMSLEDIEKDIRLMKEFNVNAVRTSHYPPDPFFLTLADVYGLYVID
                     EADIETHGVGRLTGSRDTISHDLKWAKHYVDRVKRMYYRDRNHPSVTLWSLGNEAGGY
                     KCHDKCYSYLKSVSDIPVHYEGVIDTRRHSYDVISEMYPRHDWLRKMVSKKRGKAFTG
                     KPEVLCEYVHAMGVGPGGMEEYWDIFYSSDQFMGGCIWEWADHAVYHAPDDPKYPYRY
                     TYGGDHGEKHHDGNFCVDGLFYPDRRPHTGAYQMKTIYRPLMAKAVSDDEFIFTNTNS
                     FKSSAYLDIKWELYRDGELKADGALDLDIPALESRKVKIDLPPLTDENSWHINFVYHD
                     GDRHVATEQLTLRDVPIEYEFEITDSISASRENEFLTVNFEGGKAVFNAETGELTSYV
                     KDGTEYINKTPAEGLTGLLPLVFRALLDNDRKISGEWLAAGLDKAKPVLTNFETRLLE
                     REVEVVAEFDIVAKRRILYKVKTKYIVSGFGAIEVSSELVRPKNSKAPDDIPRFGLVL
                     QLGREFNKVKYYGRGDKENLPDFKVHAPVGIYETTVEDMHEPYIYPQDNGERTEVKWL
                     SITNDKGKGLKIYADDKLAFNAHNYTVDALYKAHHQEDLEDMNTTVLTIDGAIRGTGT
                     GSCGPDTLPEYLINASKGVKFSFTILPL"
     gene            complement(3645..7217)
                     /gene="nifJ"
                     /locus_tag="PLD98_01625"
     CDS             complement(3645..7217)
                     /gene="nifJ"
                     /locus_tag="PLD98_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HQA04981.1"
                     /translation="MARKMKTMKGNSAAAYVSYAFTEVAAIYPITPSSDMAEETDKMS
                     ADGVKNIFGQTVRVAEMQSEAGAAGAVHGSLAAGALTTTYTASQGLLLMIPNMYKIAG
                     ELLPNVIHVSARAVASHALSIFGDHSDVYACRQTGYAMLCSGNVQEVMDLGAVAHLAT
                     IKSRVPFLHFFDGFRTSHEIQKIHVWDYNELREMVDMDCVNAFRRRALNPEHPVLRGS
                     AQNPDIFFQAREAVNPFYDDVVAATEKYLEEVNKRAGTNYKLFNYYGAPDADRVIIAM
                     GSVCDTIEETIDYLNANGEKVGLVKVRLYRPFSAKHLIEALPDTVKKISVLDRTKEPG
                     SLGEPLYLDVVAALRDSKFKDVEIFAGRYGLGSKDTNPSCVFAVYDNMNAAEPKKRFT
                     VGINDDVTNLSLPITNHVDTTPEGTVSCKFWGLGSDGTVSANQNSIKIIGDNTDMYAQ
                     GYFAYDSKKSGGITISHLRFGKKPIKSTYYINKADFVACHNPSYVDKYDMVQDLKKGG
                     VFLLNCGWTLDELNTHLPAKMKKYIAENDIKFYTIDATSIAKDLGLGGRVNTILQAAF
                     FKLTGIIPVDKAVDLMKEAATRSYGKKGEKIVAMNHAAIEKGIEGVVEVKVPADWANA
                     EVEEVKHEVKTDRPELKKFVEEVLIPVNAQKGDTLPVSTFKDTPDGTFPQGSAAFEKR
                     GIAVDVPNWIPENCIQCNFCSYVCPHAVIRPAVLDENELKNAPEGMETLPMTGVPGLN
                     FAITVSTLDCTGCGSCASICPGKKGAKALEMKPIEQQMPKQKYFDYGIELPAKPEVFE
                     KFKPTTVKGSQFKQPLLEFSGACAGCGETPYAKLVTQLFGDRMYIANATGCSSIWGGS
                     APSTPYTVNKEGKGPAWANSLFEDNAEYGYGMLLANTQIRNRLEECVRILAEANITEA
                     LTDAANRWLETKENGNENTAASKALLAVLSDAQKGDEKAKAAAEEILTDADFLAKKSV
                     WIFGGDGWAYDIGFGGLDHVIAMGEDVNILVFDTEVYSNTGGQQSKATPTGAIAKFAA
                     AGKSGKKKDLAQIAMSYGHVYVAQVAIGADYNQTIKAITEAESYHGPSIIIAYAPCIN
                     HGIKGGLTAGIHEEKKAVEAGYWHIFRFNPELRKEGKNPFTLDSKAPTGDYREFLLNE
                     VRFASLAKAMPERADELFKAAEESARDKYESLVRLKDFYEPKAE"
     gene            7883..9229
                     /gene="gdhA"
                     /locus_tag="PLD98_01630"
     CDS             7883..9229
                     /gene="gdhA"
                     /locus_tag="PLD98_01630"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HQA04982.1"
                     /translation="MNQYIAKVISDTRKKYAGEPEFLQAVEEVLTSLEPVIARHPEYE
                     KYNILGRMVEPERFISFRVCWTDDSGNIRVNTGYRVQFNSAIGPYKGGLRFHPSVYPG
                     IIKFLGFEQVFKNSLTGLPIGGGKGGADFDPIGKSEGEVMRFCQAFMTELYRHIGPDT
                     DVPAGDIGVGGREIGYLFGQYKRIKGNFENGVFTGKGLEYGGSLLRPESTGYGAMYFA
                     NEVLRHFGDSVEGKTIAISGYGNVAWGVAKKAAELGAKVVTISDPTGYVLDKDGICTE
                     EKFEYMNRLRYVGDVTLADYAKQFGATFYPGQKPWGEKVDIIMPCATQNEVGIEEAKR
                     IVENGVKYYVEVSNMPTSNEALAYLSKHLMAVAPAKAANAGGVAVSALEMSQNAERRI
                     WDSHTVDELLKDIMKNIHDNSMHAAEEYGFGYNLIAGANIAGFKKVADAMIAQGVI"
     gene            9444..9617
                     /locus_tag="PLD98_01635"
     CDS             9444..9617
                     /locus_tag="PLD98_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04983.1"
                     /translation="MHRDTLERLKVFLIVLAVFFVFFVASYVSKTLDKSGKAEGMGIM
                     SYAELNIGQDGNV"
     gene            9855..10544
                     /locus_tag="PLD98_01640"
     CDS             9855..10544
                     /locus_tag="PLD98_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQA04984.1"
                     /translation="MYTVLVCDDDKAILDALEIYLKLEGYNVLKAENGEEALKLASEN
                     EIHCMILDIMMPGMDGLRATLKLREIKNIPIILLSAKSEDSDKITGLSFGADDYVTKP
                     FNPLELMARVKSQIRRFTTLGSIEVKEDVLVNGGLVVDLKAHEVTVDGEPVKLTATEY
                     GIVEFLMRNVGRVFSTAQIYEAVWNEPFVAGEKTVTVHIRRIREKIEIDPKEPRYIKV
                     VWGVGYKIEKQ"
     gene            10891..13809
                     /locus_tag="PLD98_01645"
     CDS             10891..13809
                     /locus_tag="PLD98_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQA04985.1"
                     /translation="MEKGNQKSFDIKRAVFLGVILIVYFVTSLGFAPLVKSIFVYNLP
                     IPEGSAYTDTWKFYDQLGDYAYSLTTYLKFKGDEINTESEDFIDYFINEGKGSQIADT
                     YYEYASMRRLAEILAEIEKKAGKSKDGKAVPYEDFAFRLFGIKSGDEETEPQSEDHDD
                     GFRATSPAGGNAELHSAPALEYKVEYHTSPNENLTEGITYRSAQNAHSIDLWGDLSRF
                     NAEEIDYYLKKYNVVISGKEVGPINIGWAQDLLERFDEFYSDDHESNEEYAESLYELY
                     SETVRLFARRDFAEDYSPEKIIAYYAEQMKTTENKVAAELNADFYLAQQLLGSYSNFK
                     YIFYDTKTGNFESNFYEGAVNPENPLNISLNADELSWLMVYSEKSGLKIGKPENKTAS
                     ELSITPSPYSKFDLTRQAENRLKGYCSNRYILYLALPKTLVNSDLISDYSSDFEVLSK
                     SFNKNLTTFIVGMLLSIVFTIAFSLTSGESEDGGRLYFIDRIFNDVHFAASSAVIVSL
                     AITTVVLLKALMTNGALVLTSDGSPVYRFAELNRAYDIIVPAAMMISAAASLELLSSI
                     SRNTRLKRYFSHTLAAKILGFILGIFRRFWKFIKRIINFVLKPFRGLMQMASMFTTNK
                     LKTLTKSLLFYSLLYFGINAVLVLFFIASAVNVSKGGTFGSVFFTMLIGVAFNTVVLY
                     KVFMFLESLDSIIFAASQIAQGRYNIALNIPRMPRLLKSLASDLLNINAGTKAAVETA
                     VKGERLKAELITNVSHDLKTPLTSIVNYVDLLQRSGEYGEPANEYIKILKEKSNRLKA
                     LIDDLMEASKVSTGNIKINLSPVNLYELAMQISGEHSDVLEEKGIDIIIAKPEVEPIV
                     WADSQQTWRIIENLFSNVKKYTMPGTRVYVDTTIENGYGCFSIKNISADALNIPAEEL
                     TQRFVRNDEARSGEGSGLGLSIAESLCKLQKGEFSITIDGDLFKVKVCLPIYRGNQES
                     "
     gene            14062..15546
                     /locus_tag="PLD98_01650"
     CDS             14062..15546
                     /locus_tag="PLD98_01650"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HQA04986.1"
                     /translation="MRRLLDISSQRLIKILELFALGEQNLTFKDIARHVKASVRTVHD
                     DIAHIKERWGDKLSIEVSPKSGISVKGCSIFTFEKICKEIFNESQSLLLFQGIFFNPY
                     RNNEFHEAIVFASHSTLSRMIKKFNGKRVDDTGIRILKNKAGYYLYAPDEQQFRRLST
                     CFTLEIEGLDKSLFEQWLKEYQISFADDYINEIIRRINPNIDEFYDDRAFKYDATFFL
                     VSLIRELQGFHAEPYPIDYEPEITEELLRLVRKKFPAVTKNALNAICIGLKLHHTDIR
                     KDRDWHKIRPCFDTFLNNALKIIGVPAEENWLEEAYTVLGKLYNLGLWFNIKASTLFD
                     RVEYFAQLFKRKNRAVYDYLYRETEIMSAKLGVNLTFNFHKILFWLCVRIPDFSGVTI
                     KKKLLVIGDYSELHTEFLANFIEENINGAKSRKVETIPFYLGKGEPLPPPESFDIIAS
                     TMPNIASVYPELSSKGMAVVNDYPTEENIADLKNLLSGLKMQLD"
     gene            complement(15582..15836)
                     /locus_tag="PLD98_01655"
     CDS             complement(15582..15836)
                     /locus_tag="PLD98_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQA04987.1"
                     /translation="MDILQYLTEKSLVLIPALLILGQIIKGIEWIPDKYIPLILLPFG
                     ILGSVGLNTFCADTVIQGILVTGAAVFGNQIVKQLKKKEE"
     gene            complement(15865..16158)
                     /locus_tag="PLD98_01660"
     CDS             complement(15865..16158)
                     /locus_tag="PLD98_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04988.1"
                     /translation="MEISERLWEESMRSIDARFARAERDIDELYSRQCEIEKLSIQIG
                     EMLKHHEKTQEKHGKRLDNLESRPVKYFDKFISGIIGALTGSLTAALISLILK"
     gene            complement(16180..16845)
                     /locus_tag="PLD98_01665"
     CDS             complement(16180..16845)
                     /locus_tag="PLD98_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HQA04989.1"
                     /translation="MFKNNIELAAWSISKVGCPYWYGTFGQIASESLLKQKRKQYPSH
                     YTDARLAKMRSHFGKQVFDCVGLIKGYMWESGGRIVYNAAQDVSADTMYKLCKEKGDI
                     KDIPEIKGLLVFKKGHVGVYIGSGKVVEAKGFNYGVVISNLKGSGFTAWGKCPFIFYA
                     EKFDVDGDGKVTAADARAVLRHSAKLETLSAAEQYSADVNNDGKVDSKDAREILRQSA
                     GLG"
     gene            complement(16891..17721)
                     /locus_tag="PLD98_01670"
     CDS             complement(16891..17721)
                     /locus_tag="PLD98_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04990.1"
                     /translation="MIKEVKLGKEPLENIENINSNFSELSSSINNLKPGQPGGVATLD
                     ESGKLNRNQLPLIDLIPIEDSSVIYLDSFSFELPASAPDGTWGYRTANNKLYLRKDGA
                     WTGPFEPSPSKLYLWPQGRQMYYAVQGVGLMPLIFQFPNLETLLMLETSDDPFGLAIA
                     GDPMMQKYRDFWEYKDITSVRMVSGMPTSDGKVGDAAYDPQTKLMYLYGIWGWEVLRY
                     DIYTGGLEPYLIADFVEKNENLIFLDKSSSKLYSYTADYIRDGNNQITKIEVTLHPLN
                     "
     gene            complement(17735..18430)
                     /locus_tag="PLD98_01675"
     CDS             complement(17735..18430)
                     /locus_tag="PLD98_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04991.1"
                     /translation="MAMREINIEIKGETGETIVCEDAGMAGEHNAACLVFTLPAALRN
                     QDLKFYAEYQSRTGRTVRSRYLEKDASGKVIFPITAELSSQIRAECTFNAAAFEEGKL
                     VQKIKPVKLLLKFTPSPDCAVIYEESASVNFEELLEFLRNAGTGIFRYERQVDAPDWT
                     FQSGIFKTVVPENEHKLGTGASLVLSEAFDGERYKPVTCTYYRDEGGNVTVLSHVPFK
                     GRLILSLSEQKLI"
     gene            complement(18445..18669)
                     /locus_tag="PLD98_01680"
     CDS             complement(18445..18669)
                     /locus_tag="PLD98_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA04992.1"
                     /translation="MSAKSLTALADEYLESARLQTEIIRKYNERKLKAIKSRNRDELL
                     ICSRALSVLYSARRDLLDTAELLRGYYDRS"
     gene            complement(18688..19107)
                     /locus_tag="PLD98_01685"
     CDS             complement(18688..19107)
                     /locus_tag="PLD98_01685"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HQA04993.1"
                     /translation="MKRKDIHINFELIADDSQLEALRKIEEDCSDCESIGKRRLSLTE
                     LINIAIRDELTEAERKAVELFWFKGMKVSEAARYLGVSSATITKTLDRAKLRLFNALK
                     YAVLYNLDLSDCPEITENASEIAKNAEVFLKRKLKDI"
     gene            19510..21762
                     /locus_tag="PLD98_01690"
     CDS             19510..21762
                     /locus_tag="PLD98_01690"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HQA04994.1"
                     /translation="MDFPTVFIGASKEYGGFENHIPAPYIRKNFNLDYEPAEPRLLIC
                     GLGYYRVFINGKEITKGPLAPYTSNPDDYTYFDKYDLKGLIKKGENAIGIILGNGMTN
                     GFDGYVWDFDTAKHRGAPRVALSIFDEAKEEDNCLLTADTAFKTHPSPILFDSLRCGV
                     IYDARLEIEGWCEPGFDDSEWANCVIAEMPRGKAKICECEPIVVSKELKPVSVTKHMD
                     GYLYDFGVNYAGVTRIKIKGERGQTITVDHGDWLHRGLFSTENYNRFYPEGYSQKTVY
                     TLKGGGEEVFVPSFTYYGFRYAYVTGITEEQATEDLLTYLVMHSDLKEVGGFKCSDES
                     LNRLQSMVRVSDLANFFYFPTDCPHREKNGWTGDAAVSAEHMLINLTPENLYREWLYN
                     IRAAQNEEGALPGIVPTGGWGFKWGNGPCWDQVLVVLPYFVYKYRGDKKILKENAHAI
                     LRYLDYLDRNLNEKGLIDFGLGDWVHANRDAGKPVCPIEVSNTIISMNIAAMSERVFE
                     VLGLELQRQFAKGFKEKLRNNIRKHLVNFSTMTVLGRCQTAQALAIAYGVFDESERPK
                     AIEVLLKLIEEKDYYIDVGMHGLRVIFHVLSDAGYADIAYKMIARKDPPSYGHWVEQG
                     FTSLPEEFRPDYTDFATSYNHHFLGDISNWFIQGICGIKLNPHAEDVNFVRIAPEFIA
                     ELDWAEGWHKAPAGEIKVKWARDGEKVLLSVTVPEGIKGEIILPAGYTDENGYSVLKL
                     QSYENLICIK"
     gene            21901..22446
                     /locus_tag="PLD98_01695"
     CDS             21901..22446
                     /locus_tag="PLD98_01695"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpaII family restriction endonuclease"
                     /protein_id="HQA04995.1"
                     /translation="MNKINQIETRTKIIDKINRLIEFGCELKPAEHLESARFEENLQF
                     IDTMMPRLLSLAVLYSYIYKLRTSKQIIDKMKELNPLGYSNVQMYEYKYKKMLCACAL
                     GMTPEKDWEGDEDANGGYIVVKRDGTVVCYHIYNRTDFEQYLFDYTCFDKASTSRYKY
                     MDIYKDNEGYKIKLNLQVRFT"
     gene            complement(22501..>23493)
                     /locus_tag="PLD98_01700"
     CDS             complement(22501..>23493)
                     /locus_tag="PLD98_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HQA04996.1"
                     /translation="VGFAEEQYSELPYAQEFAKTIGVPNFAKKITAEEYFDIAPTVQY
                     YMDEPLPNPSALPLFFLSQNASKYVKVVLSGEGADELFGGYHYYKDPLDFESYRRIPK
                     PVRQLAAVAARALPRNIHGRRFLIRGAQDIEERYIRNNYVFDYIERNKVLNFKQKAQN
                     PAHYTKPYFDKVKHLDDITKMQYADINVWMVHDILVKADRMSMANSLEVRVPFLDREM
                     LKVALAIPSKYRVSRTNTKIVLRRAAARVLPEKTANMRKKGFATPLNDWLRQDRFYNR
                     VKESFESDIAREFFDTKYIMKLLDDHKAGRAKNMKKIWTIYSFILWYQKYFIEN"
BASE COUNT         6038 a         5813 c         5732 g         5910 t
ORIGIN      
        1 gaacaaactt taagtggaaa tatgagccat atgttaacat tctttggcat tttctttttt
       61 tcgaccgagg gaaggtttat attcttatta aaagtcatat ttgatgaatt attctgtccg
      121 ctgtggtact atctctaaag gaaaccgcct ttaaaatttt aaggggagga aaaaaattga
      181 aatacgagat taacaaaaca aatcattgta cttttccgct ttttgaaaag aacaagctgt
      241 cacccagagc ctactttatt ccctttaagg acagaaagct tcttgagaaa acggatataa
      301 gaaaagaaag atattccggc gatatggtaa aggtgctctc aggcgagtgg gattttaagt
      361 tctatcccaa aaagtcaaag atgccgagca gcatcaatac ctcaaaaata aagtttgaca
      421 aggttcaggt cccctccaca tggcagagga cgggctatat ggaccccgtt tacctcaatg
      481 tagcctacga gttcaccgca gtcccgcccg caatccccga ggatatgccc gttggacttt
      541 acagaaagct cattgacatt gaggatttgt caaagaatta tataatcacc ttccttggcg
      601 tcgcctcaag ccttgacctt tatgtaaacg gaagctttgt gggctactcc gagggctccc
      661 acaactccgc ggaattcgat attacaaagt tcctcaaaaa gggcgagaac gagattgtag
      721 ccgttgtttt caagtggagc accggcacct atcttgagtg ccaggatatg ttcagagaga
      781 acggcatatt ccgtgatgtg cttcttaccg agtacgagcc cgttttcatt tacgactact
      841 gcctcaaaac cgcaaagcag ggcgagcttt ataatctcac cgccgagatt gatattaagg
      901 gtcagcccga aggctatgat gtggagattg aaatagtaaa agacggcgcc gttatagcct
      961 ccgccgcagc ggacgccaag gagcagacgg taataaagtt ccagaacctt gatgttaagg
     1021 aatggaaccc cgaaattccc gagctttatg atgtattcat caccttaaag cagagcggca
     1081 gagaggtaat gagcatcagg aataagacgg gtttcagaac catagagatt gacggcgagg
     1141 tattcctctt taacggcaga aagataaaga tgaagggcgt caaccaccac gacacccacc
     1201 ccgtcaaggg ctacgccatg agtcttgagg atatcgaaaa ggatataagg ctcatgaagg
     1261 agttcaatgt gaacgcggta agaacctccc actatccgcc cgaccccttc ttcctcaccc
     1321 ttgccgatgt ctacggactt tatgtaattg acgaggccga tattgaaacc cacggtgtgg
     1381 gcaggctcac aggctcaagg gataccataa gccacgacct taaatgggct aagcactatg
     1441 tggacagagt caagagaatg tactaccgag acagaaacca cccctcggtc accctttggt
     1501 cactgggcaa cgaggcgggc ggctacaagt gccacgacaa gtgctacagc tatcttaaat
     1561 ccgtaagcga cattcccgtt cactacgagg gagtaatcga tacaaggcgt cattcctatg
     1621 atgtcatctc cgagatgtac ccccgccacg actggctcag aaagatggtc agcaaaaaga
     1681 ggggcaaggc gtttacaggc aagcccgaag tcctttgcga atatgtccat gccatgggcg
     1741 taggtcccgg cggcatggag gagtattggg acatcttcta ctccagtgac cagttcatgg
     1801 gcggctgcat ctgggagtgg gctgaccacg cggtctatca cgcccccgac gaccccaaat
     1861 acccttacag atacacctac ggcggcgacc acggggagaa gcatcacgac ggcaacttct
     1921 gcgttgacgg tcttttctat cccgacagaa gaccccatac aggcgcctat cagatgaaaa
     1981 ccatctacag acccctcatg gcaaaggcag tctccgacga tgagttcatc ttcacaaaca
     2041 ccaacagctt taagtcctcc gcttatcttg acatcaagtg ggagctttac cgcgacggcg
     2101 agctgaaagc cgacggcgct ctggacctcg acattcccgc ccttgagtcc cggaaggtca
     2161 aaatcgacct gccgccgctt accgacgaga acagctggca tattaacttt gtttaccacg
     2221 acggcgacag acatgttgcc accgagcagc tgacgctcag ggatgtgccg attgagtatg
     2281 agttcgaaat caccgactcc atctcagcct caagggaaaa cgaattcctt acagtcaact
     2341 ttgagggcgg caaggccgta ttcaacgccg agactggcga gcttacttcc tatgtaaagg
     2401 acggcaccga gtatataaac aagacacccg ccgagggact gaccggactt ctgccccttg
     2461 tattcagggc tctccttgac aacgacagaa agataagcgg cgagtggctg gcagcgggtc
     2521 ttgacaaggc aaagcccgtc ctcacaaatt tcgaaacccg ccttcttgag cgcgaggttg
     2581 aggtagtcgc cgagttcgat atagtcgcca agagaagaat tctctacaaa gtaaagacaa
     2641 agtacattgt tagcggtttt ggcgcaatcg aggtaagctc ggagcttgta agacccaaga
     2701 acagcaaggc tcccgacgat atacccagat tcggacttgt cctccagctg ggcagggagt
     2761 ttaacaaggt taaatactac ggcagaggag ataaagagaa cctgcccgac tttaaggttc
     2821 acgctcccgt cggcatttac gaaaccaccg tcgaggatat gcacgagcct tatatctacc
     2881 cgcaggacaa cggcgagaga accgaggtca agtggctcag cattacaaat gataagggca
     2941 agggcttaaa gatttacgca gacgacaagc tggctttcaa cgcccataac tacaccgtcg
     3001 acgccctcta taaagcccat catcaggagg accttgagga catgaacaca acggtcctga
     3061 caatcgacgg agctattaga ggcacaggca caggcagctg cggccccgac accctgcccg
     3121 agtatttaat caacgcctca aagggagtca aattcagctt caccatcctc cccctctaaa
     3181 atataatgga ctaaagggac gcactgcgtc ccttttttta tgccctattg tgtaaggtat
     3241 gcccccgctg aggacgacta ccgcccccgc ccaccgccga gtgcaatcac cgctcagcaa
     3301 aaaccgcaca ccaccccgcc gacctgccga gggatacccg ccgaaaatta agccgcaagc
     3361 cacccgcccg ccgaggggga gcggaacgcc cgtcttgaaa actgcatcca cgccccgccg
     3421 ccgcccgtgg aaaactctgc gctggacggg agcggcacta cgccgaatat gatttccttt
     3481 cccctatatt ggtcaggtag atgcgcccga cgggggaacc ccgggacaaa attatgtcgg
     3541 gcacagcgag caaaagaaaa aggcacgctt tttgagcgtg ccttcttctt tatctttaaa
     3601 gaacagtctc tgaaaccaaa gagctgtact tgaggtagct tttattactc ggccttgggc
     3661 tcgtagaagt ccttcagcct tacgaggctc tcatacttgt ctcttgcgct ctcttcggca
     3721 gccttgaaga gctcatctgc tctttcgggc atagcctttg caagcgaagc gaaacgaacc
     3781 tcgttaagca ggaactcgcg ataatcgcct gtgggagcct tgctgtcgag tgtgaacggg
     3841 ttcttgcctt ccttcctgag ttcggggttg aagcggaaga tatgccagta gcctgcctca
     3901 acagccttct tctcctcgtg gatacctgcg gttaggccgc ccttaatgcc gtggttgatg
     3961 cagggggcgt atgcgatgat gattgacgga ccgtgatagc tttcagcctc tgtgatagcc
     4021 ttgattgtct ggttgtagtc ggcgccgatg gcaacctgtg ctacatagac atggccgtag
     4081 ctcatagcta tctgagcaag gtccttcttc ttgccgctct tgcctgccgc ggcaaacttg
     4141 gcgattgcgc ctgtcggtgt agccttggac tgctgaccgc ctgtgttgga gtaaacctct
     4201 gtatcgaata caaggatgtt tacatcctcg cccattgcga ttacatggtc aagaccgccg
     4261 aagccgatgt cgtatgccca gccgtcgccg ccgaaaatcc atacggactt cttagcgagg
     4321 aagtcggcgt ctgtgaggat ttcctcagct gctgccttag ccttctcgtc gcccttctga
     4381 gcgtcggaga gaaccgcaag gagagccttg gaagcggcgg tgttctcgtt tccgttttcc
     4441 ttggtctcga gccatctgtt ggcagcatcg gtcagagcct cggtgatgtt ggcctcggca
     4501 aggattctta cgcactcctc aagcctgttc ctgatctgag tgtttgcaag cagcatgccg
     4561 tagccgtact cagcgttgtc ctcaaacagg gagtttgccc atgcggggcc cttgccctct
     4621 ttgttgacgg tgtagggtgt gctgggcgcg ctgccgcccc agatggatga gcagcctgtt
     4681 gcgttggcga tatacattct gtcgccgaag agctgagtta caagctttgc gtacggagtc
     4741 tcgccgcagc ctgcgcatgc gccggagaac tcaaggagcg gctgcttgaa ctggctgccc
     4801 ttaacggttg tgggcttgaa cttctcgaat acctcgggct ttgcgggcag ctcaatgccg
     4861 tagtcgaagt acttctgctt gggcatctgc tgctcaatgg gcttcatctc aagagccttt
     4921 gcgcccttct tgccggggca gatgcttgcg catgagccgc agcctgtgca gtcgagcgtg
     4981 gatactgtga tagcgaagtt aagtccggga acgcctgtca tcgggagtgt ctccattccc
     5041 tcgggagcgt tcttcagttc gttctcgtca agaaccgcgg gacggataac cgcgtgcggg
     5101 catacatagg agcagaagtt gcactgtatg cagttttcgg gtatccagtt gggaacatcg
     5161 acggcaatgc ctctcttctc gaatgcggcg ctgccctgcg ggaatgttcc gtcgggtgta
     5221 tccttgaatg ttgaaacggg gagggtgtcg cccttctgag cgttaacggg gataagaacc
     5281 tcctcgacga acttcttgag ctcgggacgg tctgtcttaa cctcgtgctt gacttcttca
     5341 acttcagcgt ttgcccagtc ggcgggaacc ttaacctcta caacgccctc aatacccttt
     5401 tcgatagcgg cgtggttcat ggcgacaatc ttctcgccct tcttgccgta ggagcgggtt
     5461 gccgcttcct tcataaggtc aacagccttg tcaacgggga taatgcccgt cagtttaaag
     5521 aatgctgcct gaaggattgt gttgactctg ccgccgagac ccaagtcttt tgctattgag
     5581 gtagcgtcga tggtgtagaa cttaatgtcg ttctcggcaa tatacttctt catctttgcg
     5641 gggaggtgag tgttgagctc gtccagtgtc cagccgcagt tcagaaggaa tacgccgccc
     5701 ttcttgagat cctgtaccat gtcgtacttg tcgacatagg acgggttgtg gcatgccacg
     5761 aagtcagcct tgttgatgta gtaggttgac ttgatgggct ttttgccgaa tctgaggtgg
     5821 gatattgtaa tgccgccgga cttcttggag tcgtatgcga agtagccctg agcgtacata
     5881 tctgtgttgt cgccgataat cttgatggag ttctggtttg cgctgacggt accgtcggag
     5941 ccgagacccc agaacttgca ggaaactgtg ccctcgggag tggtgtcaac atggtttgtg
     6001 atgggcagtg agagatttgt tacatcgtcg ttgatgccta cggtaaatct cttcttcggc
     6061 tcagcagcgt tcatgttgtc ataaacggcg aatacgcagg aggggttggt gtcctttgag
     6121 ccgagaccgt agcggcctgc gaagatttca acatccttga acttggaatc tctgagagcc
     6181 gcaacaacat caagatacag aggctcgcca agggagccgg gctcctttgt tctgtcaaga
     6241 acggaaatct tcttaacagt gtcgggaaga gcctcgataa gatgctttgc ggagaaggga
     6301 cgatagagac gaacctttac aaggccgacc ttctcgccgt ttgcgttgag ataatcgatg
     6361 gtttcttcta ttgtatcgca aacagagccc attgcgatga tgactctgtc agcgtcgggc
     6421 gcgccgtagt agttaaagag cttgtagttt gtgcctgctc tcttgttaac ctcttcaagg
     6481 tatttctctg ttgcggctac aacatcgtca tagaaggggt taacagcttc tctggcctgg
     6541 aagaagatgt ccgggttctg agcggagccg cggagaacgg ggtgctcggg gttaagagcg
     6601 cggcggcgga atgcgttaac gcagtccatg tcaaccattt ctctcagctc attgtagtcc
     6661 caaacatgaa tcttctgaat ctcgtgggag gtgcggaagc cgtcaaagaa gtgcaggaac
     6721 ggaacgcggc tcttgattgt tgcgaggtga gctactgcgc ccaggtccat aacttcctga
     6781 acattgccgg agcagagcat tgcgtagcct gtctgacggc aagcgtaaac atccgagtgg
     6841 tcgccgaaga tgctcagcgc gtggctggcg actgcgcgtg ccgaaacatg tataacattg
     6901 ggcagaagct cgcctgcgat tttatacatg ttcggaatca taagaagcag accctgggat
     6961 gcggtgtagg tggtggtgag agcgcctgcc gcaagggagc cgtgaacggc gcctgctgcg
     7021 cctgcctcgg actgcatttc agcaaccctg acagtttgtc caaaaatgtt tttcactccg
     7081 tcagcggaca ttttgtcggt ttcttccgcc atgtccgaag aaggagtgat aggataaatt
     7141 gccgcgacct cagtaaacgc gtatgataca tacgcggccg cagaattacc cttcattgtc
     7201 ttcatttttc tggccatttt atattgtttc ctcctgtttt gatttgctga ctgatctatt
     7261 tcctgcgccg cccctcggtt tcacagagag gccaaaacgg gaattgaaca cacaaagatg
     7321 tcggaactga gaacagcctc ccatatgggg aaaaacgatt tcgttccgac cgttattttt
     7381 ataacaaaga tagtttaaca cctttttgag ccgttgtaaa tactgagact tatactttta
     7441 cattattcca gaaaatcctg ttaaaaaaca taaaaacttg gcaaaataag cggaaattta
     7501 agctaattat atcatatttt catttatata tagattatct aaattaagga aatttttcaa
     7561 tatgaaaatt aaaatttccc ggcgatttat caggaaaaag cgtcatccga aagtcaaaga
     7621 gaagaaaatg tgtggaatta catttaattc cgccgcaaat gttaaatctt tttgtgaaaa
     7681 atataaaatt tttttcaaat caaccccatg gtttgtagct ttatacgaat tgtatacaat
     7741 ctttggtttt tcattgtatt ttaagaaaaa taaaggtaca atacagattg ccgaaatctg
     7801 cgtattttgc ggaattttgt tttatggttg gtttattgtt ggtattattt cattcagcga
     7861 atttgaaggg gctaatttca aaatgaatca gtacattgcg aaagttatca gcgacacaag
     7921 aaaaaaatac gccggcgaac ctgagtttct gcaggctgtg gaggaggttt taacttccct
     7981 cgagcctgta atcgcgcgcc atcccgaata tgaaaagtac aatattctgg gacgcatggt
     8041 ggagcctgag cgctttatat ctttcagggt ttgctggacg gacgacagcg gaaacataag
     8101 ggtaaacaca ggctacagag tacagttcaa cagcgccata ggaccttata agggcggact
     8161 gcgctttcat ccttcggttt atccgggcat tatcaaattc ctcggttttg agcaggtctt
     8221 taaaaacagc cttacaggcc tgcccatagg cggcggcaag ggcggcgcgg actttgaccc
     8281 catcggcaag agcgagggcg aggttatgcg cttctgtcag gcgtttatga ccgagcttta
     8341 ccgccacatc ggacccgaca ccgatgttcc cgccggcgac atcggcgtgg gcggcagaga
     8401 aataggctat cttttcggac agtacaagag aatcaagggc aatttcgaaa acggcgtatt
     8461 cacaggcaag ggtcttgagt acggcggctc tcttctcaga cccgagtcaa cgggctacgg
     8521 cgccatgtac ttcgccaacg aagttctcag gcacttcggc gacagcgtag agggcaagac
     8581 cattgcgatt tccggctatg gcaatgtggc atggggcgtc gctaaaaagg ctgcagagct
     8641 tggcgcgaag gttgtcacca tctccgaccc gacaggctat gtccttgaca aagacggcat
     8701 ttgcaccgag gagaagttcg agtacatgaa taggctccgc tatgtgggcg atgtaacgct
     8761 tgccgactac gcaaaacagt tcggagctac cttctatccg ggtcagaaac cctggggcga
     8821 aaaggttgac atcatcatgc cctgcgcgac acagaacgaa gtcggcattg aagaggcaaa
     8881 aagaattgtt gagaacggcg taaagtacta tgtcgaggtc tccaatatgc ccacctccaa
     8941 tgaggctctc gcttatctct caaagcacct tatggcggtc gctcccgcaa aggcggcaaa
     9001 cgcgggcggc gtagcggttt ccgctcttga gatgagccag aacgccgagc gcaggatctg
     9061 ggacagccac acagtcgacg agctgctcaa ggatattatg aagaatatcc acgacaacag
     9121 catgcacgcc gccgaggagt acggtttcgg ctacaacctt attgccggcg ccaacatcgc
     9181 gggctttaaa aaggtcgccg acgccatgat agctcagggc gtaatctaag gcgagcgaga
     9241 ttcgcagcct taatcggctg cgggcgataa aagcaaaata aaagaccgag taaaactcgg
     9301 tcttttttat ctaaaagtta tgtttataat aactgtataa atacaaactg caaacaattt
     9361 atgaattccc gctgttatgt ttactttatt ttcttttcat ggtattattt ggcctgcact
     9421 atctttagaa aagaggatac ttgatgcata gggacacttt ggaaaggctg aaggtattcc
     9481 tcatagtcct tgccgttttc tttgtctttt tcgtcgcgag ctatgtctcg aaaacactgg
     9541 acaaatcggg caaagcggaa ggcatgggta ttatgtctta cgcggagctg aatatagggc
     9601 aggacgggaa tgtttgacgc ctttatatcc aaagaagaaa gaatcggcat ttatgccgat
     9661 ttttcttttt cgacatatag atacattttg tcgctgaatg ttaccaaatc ttaataattt
     9721 tacgccctct ttcttagaaa tcatattgga taataattac gaagataaat cagtgcactt
     9781 tgagataaat tcatgtataa ttgttataac cgaaaacttt gtgatttcgg gaatctgttg
     9841 ggcggaggaa ttacatgtat actgttcttg tttgcgacga cgacaaggca atacttgacg
     9901 cattggagat atatctgaag ctggagggct acaatgtcct gaaggctgag aacggcgagg
     9961 aggcgttaaa gcttgcctcg gagaacgaaa tccactgcat gattctcgac atcatgatgc
    10021 cgggcatgga cggactcaga gctacgctga aactcaggga aatcaaaaat attcccatca
    10081 tcctgctttc ggcaaagagc gaggacagcg acaagattac gggactgtcc ttcggagccg
    10141 acgactatgt gacaaagccc tttaaccccc ttgagctgat ggcgagggtc aaatcccaaa
    10201 tcagaaggtt caccactctg ggcagcatag aggtcaagga ggatgtgctc gtcaacggcg
    10261 gtcttgtggt tgaccttaaa gcccatgagg ttaccgtgga cggcgagcct gtgaagctga
    10321 ccgccaccga gtacggaatc gttgagttcc ttatgaggaa tgtgggcagg gtattctcca
    10381 ccgcccagat ttacgaggcg gtctggaacg agccctttgt ggcgggcgaa aaaaccgtta
    10441 ccgtgcacat aagacgcatc agagaaaaga ttgaaataga ccccaaggaa cctcgctaca
    10501 taaaggtcgt atggggtgtg ggatataaaa tagagaagca gtagaaagta aaaccgactt
    10561 tttcgcgcac tcccgtaaat tcacactcct ttggcaaatc tcatcctgca gcccccgcac
    10621 ccctgcgcca ttatgaaagg tttcaacgct tgcccgtcgg cagggcgaaa acccaatcga
    10681 cgggagcggc aaaccgctcc aaaccaaaac ctattttcag aacacgctcc gccctttttc
    10741 gggcggtact ttaaatttca ccccgcccga aaaagagggc gcgccgcaaa ccctattaat
    10801 aaaaacagaa cccaacggca ttaagccccc aatcgcccgc ggcaaatcgg ccgcctgctc
    10861 actgcattta atgatatttc ggaggctatt atggaaaagg gcaatcaaaa gagctttgat
    10921 ataaaaaggg ctgtattttt aggcgtgata ctcatcgtct attttgtcac atccctcggc
    10981 tttgcgcccc ttgtgaagtc tatattcgtc tacaatcttc ccattcccga aggctcagcc
    11041 tacaccgaca catggaagtt ttacgaccag ctcggcgact acgcctacag ccttaccaca
    11101 tatttaaaat ttaaaggcga tgaaataaac accgagtccg aggattttat agattacttt
    11161 ataaacgaag gcaaaggcag ccagattgcc gacacctact atgaatacgc ctccatgcgg
    11221 cgccttgcgg agattctggc ggagattgag aaaaaagccg gcaagtcaaa agacggcaag
    11281 gccgtgccct atgaggactt tgcattcagg cttttcggca tcaaaagcgg cgatgaggaa
    11341 acggagccgc aaagcgaaga ccatgacgac ggtttccgcg ccacatcccc cgccggcggt
    11401 aatgctgagc ttcactccgc ccccgccctc gagtataaag tcgaatacca tacctctccc
    11461 aacgaaaatc tcaccgaagg cataacatac cgctccgctc aaaacgcgca ctcaattgac
    11521 ctctggggcg atttgagcag attcaacgcc gaggaaatag actattacct taaaaaatac
    11581 aatgtggtca tatcgggcaa ggaagtggga cccattaata tcggctgggc tcaggatttg
    11641 ctggaacgct ttgacgagtt ttactccgac gaccatgagt ctaacgagga atacgcggag
    11701 tccctttatg agctgtattc cgaaaccgta aggcttttcg cccgcagaga ttttgcggag
    11761 gattattccc ccgagaaaat tatcgcttac tacgcggagc aaatgaaaac gacggagaac
    11821 aaagtcgccg cggagctgaa tgccgacttc tacctcgctc agcagcttct cggcagctac
    11881 tccaacttta aatacatctt ctatgacacc aagacgggaa actttgagag caatttctat
    11941 gagggcgctg taaatcccga aaacccccta aatattagcc tcaacgccga cgagctttcg
    12001 tggctcatgg tttacagcga aaaaagcggg cttaaaatag gaaagcccga gaacaaaacc
    12061 gcctccgagc tttcaataac cccctccccg tacagcaagt tcgaccttac aaggcaggcg
    12121 gagaacaggc tgaagggcta ctgctcaaac aggtatattc tgtatctcgc cctgccaaag
    12181 actctcgtaa attctgactt aatcagcgac tattcctcgg actttgaggt tctctcaaag
    12241 agtttcaata aaaatctcac cacatttata gtgggtatgc tcctctccat tgttttcaca
    12301 atcgcgttca gtttaacatc gggagagagc gaggacggcg gaagactgta ctttatagac
    12361 cgaatattca acgatgtgca ttttgccgcg tcatcggcgg tcatagtgtc ccttgcgata
    12421 actacggttg tcctgttaaa agcgcttatg accaacggcg ctttggtgct taccagcgac
    12481 ggttcccccg tttaccgctt tgcggagctg aacagagcat acgacataat cgtgcccgcc
    12541 gccatgatga tttccgcggc agcctccctt gagcttttaa gctccatttc aaggaataca
    12601 aggcttaaaa ggtatttctc ccacactctg gcggcaaaga tactcggctt tattctgggg
    12661 atattcagaa ggttctggaa gtttataaag aggataataa acttcgtgct gaagcctttc
    12721 agaggtctta tgcaaatggc gtcgatgttt acaaccaata aactaaagac gcttacaaag
    12781 agccttcttt tctactccct tctttacttc ggcattaacg cggttcttgt cttgttcttc
    12841 atcgcgtctg ctgtcaatgt ctcaaagggc ggcacattcg ggtcagtgtt ctttactatg
    12901 ttaatcggcg tcgcctttaa taccgtggtg ctttacaagg tctttatgtt ccttgaaagc
    12961 ctcgacagca taattttcgc cgcctcccaa atagctcagg gcagatacaa tatcgccctt
    13021 aacattccga gaatgccccg ccttttaaaa agccttgcct cggatttgct caacatcaac
    13081 gccggcacaa aggccgcggt agaaaccgcc gttaaaggcg aaaggctaaa ggctgagctt
    13141 atcacgaatg tctcccacga ccttaaaacg ccgctgacat ctatagtgaa ctatgtggat
    13201 ttgctccagc gcagcggcga gtacggcgag cccgccaacg agtatattaa gatactcaag
    13261 gaaaaatcca atcgtctcaa agctctcatc gatgacctta tggaggcatc aaaggtatca
    13321 acgggcaata taaagataaa tctctccccc gtaaacctct atgagctggc gatgcagata
    13381 agcggcgagc acagcgatgt gcttgaggaa aagggcatag acataataat cgcgaagccc
    13441 gaagtcgagc ccatagtctg ggccgacagc cagcagacat ggagaataat tgagaacctt
    13501 ttctccaatg tcaaaaagta caccatgccc ggcacaaggg tttatgtgga caccaccata
    13561 gagaacggct acggctgttt cagcatcaaa aacatctcgg ccgacgccct taatatcccc
    13621 gccgaggagc tgacgcagag atttgtcaga aacgacgagg caagaagcgg cgagggcagc
    13681 ggtctgggtc tgtcgatagc cgagagcctt tgcaagctcc agaagggaga gttctccatc
    13741 accattgacg gcgacctctt taaagttaag gtatgcctcc cgatatacag gggcaatcaa
    13801 gagtcctgac aaaactatca aaaggcgtcc tttaaagggc gccttttttg ttctctacaa
    13861 aaaagtactg gttttttcga tttaggtatt ataataggga tgggtaattt ttagcatttt
    13921 aaaccaactt aacgcttaaa tgtaaatatt tcgtaaattc gtgtcgattg ccgatttgac
    13981 agatttaagt gttgtggtac aatagccttg aattcgcagc tcttaaagct cacttactct
    14041 ttaagggaag agatgatggg aatgcgcaga ctgctggaca tttcatcgca aaggcttatt
    14101 aaaattcttg agctttttgc tctcggcgag cagaatctca ctttcaagga tatagcaagg
    14161 catgtaaagg cgagcgtaag aacggttcac gacgacatcg cccacataaa ggaaagatgg
    14221 ggcgacaagc tcagtattga ggtttcgccc aaaagcggta tttccgttaa aggctgcagt
    14281 attttcacct ttgagaaaat ctgcaaggaa atctttaacg aatcccaatc tctcctttta
    14341 ttccagggca tattcttcaa cccgtacagg aataacgagt tccatgaggc aattgtattt
    14401 gccagccact ccaccctttc aaggatgata aagaagttca acggcaaaag ggtggacgat
    14461 acgggcatca ggatactcaa aaacaaggcg ggttactacc tttatgcccc ggacgagcag
    14521 cagttcagga gacttagcac ctgctttacc cttgagatag aggggcttga caagagcctt
    14581 ttcgagcagt ggcttaaaga gtatcagatt agctttgccg acgactacat aaacgagatt
    14641 ataaggcgca ttaatcccaa catagacgag ttttacgacg acagggcgtt taagtacgac
    14701 gccaccttct tccttgtgtc ccttataagg gagctgcagg gtttccacgc cgagccctat
    14761 cccattgact acgagccgga gattaccgag gagcttttaa ggcttgttag aaaaaaattc
    14821 cccgcagtca ccaaaaacgc cctgaacgca atctgcattg gtctaaagct ccaccatacg
    14881 gatataagaa aagacaggga ctggcataaa atccgcccct gctttgacac attcttaaac
    14941 aacgccctta aaattattgg cgtgcccgcc gaggaaaatt ggctggagga agcctacacc
    15001 gttctgggca agctgtataa tctggggctt tggttcaata ttaaggcgtc caccctcttt
    15061 gacagagtgg agtatttcgc tcagctcttt aagagaaaga acagagcggt ttatgattac
    15121 ctctaccgcg aaaccgagat aatgtccgca aagctggggg taaaccttac ctttaatttc
    15181 cacaagattc ttttctggct ctgcgtcaga atccccgact tttcgggcgt gaccattaag
    15241 aaaaagctcc tagtgatagg cgattacagc gagctgcaca ccgagttctt ggcaaacttt
    15301 attgaggaga acataaacgg cgcgaagagc cgcaaggttg agacaatacc cttctatctc
    15361 ggcaagggcg agccgctgcc gccgcccgaa agtttcgaca ttatagcctc cacaatgccg
    15421 aatatcgcct ccgtttatcc ggagctgagc agtaagggca tggccgttgt aaacgactac
    15481 ccgacggagg agaacatagc ggatttaaaa aacttgctaa gcggtcttaa aatgcagtta
    15541 gactaaaaag gaatagggcg gggaaaaccc cgcccttatt tttattcttc cttcttttta
    15601 agctgtttta caatctggtt gccgaaaacc gccgctccgg ttaccagaat tccttgaatt
    15661 acagtgtcgg cgcagaatgt gttaaggccg acggagccca aaatgccgaa gggcagaaga
    15721 atcaaaggaa tgtatttgtc gggtatccat tctattccct tgatgatttg tcccaatatc
    15781 aaaagtgccg gaatcagcac aagggatttt tccgtaaggt attgaagtat atccatagga
    15841 acctcctaaa aagtaaagtt agctctattt cagtatcaga gatatcaatg ctgccgtcaa
    15901 agaacctgta agggcgccga taatgccgct gatgaattta tcgaagtatt tcacgggcct
    15961 gctttcaaga ttgtcaaggc gctttccgtg cttctcctga gtcttttcgt ggtgcttgag
    16021 catttctcca atctgaatcg agagcttttc aatctcgcac tgacgggaat agagctcgtc
    16081 tatatctctt tcagctcttg caaagcgggc gtctatgctc cgcatgcttt cttcccaaag
    16141 tcgttccgaa atttccatct gtccacctcg tttatgcggt taaccaaggc cggcagattg
    16201 tctaagtatc tctcttgcgt ctttagagtc aaccttgccg tcattgttaa catctgccga
    16261 atattgctcc gcggcgctga gagtctccag cttcgcgctg tgccgtaaaa cggctctcgc
    16321 gtccgcggca gtaactttgc cgtcgccgtc aacatcaaac ttttccgcat aaaagataaa
    16381 gggacatttt ccccaggcgg taaagccgct gcctttaagg tttgatatta ccacgccgta
    16441 gttaaagcct ttggcctcca ctaccttgcc tgagcctata tacactccca catggccctt
    16501 tttgaacact agcaggccct ttatttcggg aatgtccttt atatccccct tttccttgca
    16561 gagcttatac attgtgtcgg cgcttacatc ctgagcggcg ttgtaaacta ttctgccgcc
    16621 gctttcccac atatacccct taatgagtcc cacgcagtca aagacctgct tgccgaaatg
    16681 gctgcgcatt ttggcgagtc tcgcgtctgt gtagtgggag gggtactgct ttcgcttctg
    16741 tttaagcagc gattcggacg caatctgtcc gaaggtgccg taccaatagg ggcagcccac
    16801 ctttgagatg ctccacgctg ctaattctat attattctta aacatcgggc ttgctccttt
    16861 aaaagaataa atcgcaaaaa gagctaaaag tcagttcaga gggtgtaatg taacctctat
    16921 ctttgtaatc tgattattcc cgtcacggat atagtccgct gtgtacgaat agagcttgga
    16981 acttgatttg tccagaaaga taagattctc attcttctcc acaaagtccg cgataagata
    17041 gggctcaaga ccgccggtgt aaatgtcgta tctcagcacc tcccagcccc atatgccgta
    17101 aaggtacata agtttagtct gcgggtcgta tgcggcgtcg cccaccttgc cgtcacttgt
    17161 cggcatgccg cttaccatcc tcacgcttgt aatgtccttg tactcccaaa agtccctgta
    17221 cttttgcatc attgggtcac ccgctatggc gagaccaaag gggtcatccg aggtttccag
    17281 catcagcagg gtctcaaggt tggggaactg gaaaataaga ggcatgagcc ctacgccctg
    17341 aaccgcgtag tacatctgcc tgccctgagg ccagagatac agctttgaag ggctcggctc
    17401 aaatggtccc gtccatgccc cgtccttgcg gagatacagc ttgttgttag ccgttctgta
    17461 gccccatgtg ccgtcgggag cggaggcggg cagctcaaag gagaaagagt caaggtaaat
    17521 tacgctgctg tcctctatgg ggataaggtc gattaggggg agctgatttc tgttcagctt
    17581 tccgctttcg tcaagcgtgg cgactcctcc cggctgaccg ggcttcagat tatttatgct
    17641 gctgctcagc tcgctgaaat tcgagttgat attttcaata ttctccaaag gctcttttcc
    17701 gagctttact tcttttatca taagccgcct cctttcatat taacttctgt tccgacaggg
    17761 acaagatgag cctgcctttg aagggcacat gggagagcac cgttacattt ccgccttcgt
    17821 ccctgtaata tgtgcaggtc acgggtttgt atctttctcc gtcaaaggct tccgaaagga
    17881 caagggatgc ccctgttccc agtttatgtt cgttttcggg aacgacggtt ttaaaaatac
    17941 cgctttggaa agtccagtcg ggagcgtcaa cctgcctttc gtacctgaaa atgcccgttc
    18001 ccgcgtttct caaaaactcc aaaagctcct cgaaattcac actggcggat tcctcataga
    18061 ttaccgcgca gtcgggggag ggggtaaact ttaaaagcag ctttacgggt tttattttct
    18121 gaacgagttt tccctcctca aaagccgccg cgttgaaggt gcattccgct cttatctgag
    18181 agcttaattc cgcggttatg gggaaaatga cttttccgga ggcatccttt tcgagataac
    18241 ggctccttac cgttctgccc gttctgcttt gatattccgc atagaacttt aaatcctgat
    18301 ttctcaaagc ggcgggcagg gtaaagacta aacacgccgc attgtgctca cccgccattc
    18361 ccgcgtcttc gcatactatg gtttcgccgg tttcaccttt gatttcaatg tttatctctc
    18421 tcatcgccat atcaaaacct cttttcagct tcggtcatag tagccgcgaa ggagttcggc
    18481 tgtgtccaaa aggtctcgcc ttgcggaata cagtacgctc agcgcccttg agcagataag
    18541 cagctcatcc ctgttgcggc tctttatggc ctttagcttt ctttcgttgt atttccttat
    18601 aatctccgtt tgcagccttg cgctttcgag atattcgtcc gccaaggcag taaggctttt
    18661 cgcgctcaaa ttgattcgcc cctttgctta aatgtccttg agtttgcgct ttaaaaaaac
    18721 ttcagcgttc tttgctatct ccgaggcgtt ttcggtgatt tcagggcagt cggaaaggtc
    18781 aagattatac agaaccgcgt atttaagggc gttgaagagc ctgagctttg ccctgtcaag
    18841 agtctttgtg atagtcgcgc tgctgactcc cagatacctt gccgcctcgc tgaccttcat
    18901 acccttaaac cagaaaagct ccaccgcctt tctttccgcc tcggtaagct cgtcgcgtat
    18961 ggctatgttt ataagctccg tcagactgag cctgcgtttg cctattgatt cgcagtcgga
    19021 gcagtcctcc tcgatttttc tcagagcctc cagctgcgaa tcgtctgcta tcagctcgaa
    19081 attaatgtga atgtctttgc gcttcatttc ttcatcctct ctttttgaag gtcgcccaat
    19141 ggggcaaagg gcaaaaaagg ataaaataaa aagacctaca attgcatagc gcagttgcag
    19201 attattctcc cgacatacgg ggcaatatcg ggttttgttt cggcggcgcc cgccgcctca
    19261 aatgacggga ggcggtcctc ccattttcgg tttgcaaaac gaacatttgt tcggttcaat
    19321 tttattgtat cggcgaaagg ggtagttgtc aatagtgagg cttctgcaaa ttctcaattt
    19381 tcctgtaatt ttccgtaatt gtcccttgat tggtactata acggcatttt ctttatttcc
    19441 tttttcttct tgcttttgat atgccgctca tgtatactca gtttagattt ttaaaaacgg
    19501 aggaaaatta tggacttccc gactgttttc ataggcgcgt caaaagagta cggcggcttt
    19561 gaaaatcata tacccgcccc gtatataaga aaaaatttca acctcgatta tgagccggcc
    19621 gagccaaggc tcttaatctg cggactcggc tattacagag tctttattaa cggcaaggaa
    19681 atcaccaagg gtccccttgc gccctatacc tctaatcccg acgactacac ctattttgat
    19741 aagtacgatt tgaaagggct tataaaaaag ggcgagaacg ccataggaat tatcctcggc
    19801 aacggcatga caaacggctt tgacggctat gtctgggact ttgataccgc aaagcacagg
    19861 ggagcgccga gagtcgccct ctcaatcttt gacgaggcaa aagaggagga taactgcctt
    19921 ctcactgccg ataccgcatt taaaacccac ccgtcgccga ttctctttga ttctctgcgc
    19981 tgcggcgtga tttacgacgc aaggctggag attgaaggct ggtgcgagcc gggctttgac
    20041 gattccgagt gggcaaactg cgttatagcc gaaatgcccc gcggcaaggc aaaaatctgc
    20101 gagtgcgagc caattgtggt aagcaaggaa ctaaagcccg tttccgtcac aaagcatatg
    20161 gacggctacc tttatgactt tggcgtcaac tacgcgggcg tgacaaggat aaaaatcaag
    20221 ggcgagaggg gtcaaaccat caccgtcgac cacggcgact ggctacacag aggcctgttc
    20281 agcacggaga attacaacag gttctacccc gaaggctatt cacaaaagac cgtctatacc
    20341 ttaaagggcg gcggcgagga agtctttgtt ccttcgttca catactacgg cttcagatac
    20401 gcctatgtaa cgggcattac cgaggagcag gccaccgagg acctcttgac ctaccttgtt
    20461 atgcactcgg atttaaagga ggtcggcggt tttaagtgct ccgacgagag cttaaacagg
    20521 cttcagagca tggtcagggt ctccgacctt gctaacttct tctacttccc cacagactgc
    20581 ccccacaggg aaaagaacgg ctggaccggc gacgccgccg tttccgccga gcatatgctc
    20641 attaacctta cccccgaaaa cctttacaga gagtggctgt ataacatcag agccgcccag
    20701 aacgaggagg gagcgctgcc gggcattgtg cccacaggcg gctggggctt taagtggggc
    20761 aacggcccct gctgggacca ggtgctcgta gtccttccct actttgtcta caagtacagg
    20821 ggcgataaga agattcttaa agagaacgcc cacgcaatac tgaggtactt ggattatctt
    20881 gacaggaacc ttaacgaaaa gggtcttata gactttggtc tgggcgactg ggtacacgca
    20941 aaccgcgacg cgggcaagcc cgtatgcccc atcgaggtat ccaacaccat aatttcaatg
    21001 aacatagccg ctatgtccga gcgggttttc gaggttctgg gacttgagct tcaaaggcag
    21061 ttcgcaaagg gctttaagga aaagctgagg aacaatatca gaaaacacct tgtaaacttc
    21121 tccaccatga ccgttttggg cagatgccag accgctcaag ccctggcaat cgcctacggc
    21181 gtctttgacg agtccgaaag accgaaggcc atcgaggtcc tcttaaagct gattgaagaa
    21241 aaggattatt atatcgatgt gggcatgcac ggtctcagag tgattttcca tgtcctctcc
    21301 gacgcgggat atgcggatat agcctacaag atgatagcaa ggaaagaccc gccgtcatac
    21361 ggtcactggg ttgagcaggg ctttacatct ctccccgagg agttccgccc cgactacacc
    21421 gactttgcca catcatacaa tcatcacttc cttggcgaca tcagcaactg gttcattcag
    21481 ggcatctgcg gcataaagct caatccccat gcggaggatg taaactttgt gagaatcgcc
    21541 cccgagttta ttgcagagct tgactgggcg gagggctggc acaaggctcc cgccggcgaa
    21601 atcaaggtca agtgggcaag agatggagaa aaagtgctgc tctccgtcac agtacccgag
    21661 ggcattaagg gagaaatcat cctccccgca ggctacaccg acgaaaacgg ttacagcgta
    21721 ttaaaactcc aatcctatga aaaccttatt tgcattaaat agcgaattct ttttgtggaa
    21781 aacctaattt atctattaat ttctgaatgc ccatagaaag acaaaaagac tgattccgtt
    21841 tgtttgcgga gtcggtcttt cttttttaat gattgaattt gttaacagaa cactttaatt
    21901 atgaataaga ttaatcaaat tgaaacccgt acaaaaatta tcgataaaat taacagatta
    21961 atcgaattcg gttgtgagtt gaagcccgct gagcaccttg agtccgcaag gtttgaggaa
    22021 aaccttcaat ttatagacac catgatgccc cgtttgttgt ctttggcagt tttgtattct
    22081 tatatataca aactcaggac atcaaagcaa ataatcgata agatgaaaga attaaacccc
    22141 ctcggatact caaatgttca aatgtacgag tataaatata aaaaaatgtt atgtgcatgc
    22201 gctctcggca tgacacccga aaaggattgg gaaggtgatg aggacgcaaa cggcggatac
    22261 atagtcgtta aaagagacgg aacggttgta tgctaccata tatacaacag aacggatttt
    22321 gagcagtacc tgtttgatta cacatgtttc gacaaagcaa gtacgagccg atacaaatat
    22381 atggacatat ataaagataa cgaagggtac aaaattaaat taaatttaca ggtaagattc
    22441 acataaaacg ctttaagctg ttataaaaac cccctccgta aacgggaggg ggttaagctt
    22501 tcagttttca ataaaatact tctgatacca cagaataaac gagtatatcg tccagatttt
    22561 tttcatgttc ttggctctgc cggctttgtg gtcgtcaagg agtttcatta tatactttgt
    22621 atcgaaaaac tctcttgcta tgtcgctttc aaagctctcc ttgacgcgat tatagaacct
    22681 gtcttgtctg agccagtcgt tgaggggagt cgcaaagccc ttctttcgca tattagcggt
    22741 tttttcgggc agaactcttg cggcggcgcg ccttaaaact atctttgtgt tggtcctcga
    22801 taccctgtat tttgagggaa tggcgagggc tactttcagc atctccctgt caaggaaggg
    22861 cacccttacc tcaagggagt tcgccatgct catgcggtcc gcctttacga gaatatcgtg
    22921 aaccatccag acatttatgt cggcgtactg catttttgtt atgtcgtcaa ggtgctttac
    22981 cttgtcaaaa tagggctttg tgtagtgcgc gggattctga gccttctgct taaaattcag
    23041 caccttgttg cgctcaatat agtcaaagac atagttgttt ctgatgtatc tttcctctat
    23101 gtcctgagcg ccccttataa ggaatctcct gccgtgaatg ttcctcggca gagccctcgc
    23161 ggcgacggcg gccagctgcc ttacaggctt tggaattctc ctgtagcttt caaaatccag
    23221 agggtccttg tagtaatgat agccgccgaa aagctcgtca gcgccctctc ccgagaggac
    23281 aacctttaca tacttggagg cgttctggga aaggaagaac agcggcagag ccgacgggtt
    23341 ggggagcggc tcgtccatat agtactgaac ggtgggggcg atatcgaaat attcctcagc
    23401 ggtgattttc ttcgcgaagt tgggaacgcc gatggtcttt gcgaactcct gagcataggg
    23461 cagctcgctg tactgctcct ccgcaaagcc tac
//
LOCUS       DAONVQ010000012        22858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_1594_length_22858_cov_5.004342, whole genome shotgun sequence.
ACCESSION   DAONVQ010000012 DAONVQ010000000
VERSION     DAONVQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 22858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22858
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1594_length_22858_cov_5.004342"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /gene="ftsH"
                     /locus_tag="PLD98_01705"
     CDS             <1..1382
                     /gene="ftsH"
                     /locus_tag="PLD98_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQA04997.1"
                     /translation="GSDFLEMYVGVGAARVRDLFNQAKKSAPCIVFIDEIDAVGRHRG
                     AGMGGGHDEREQTLNQLLVEMDGFVENEGVIVLAATNRPDILDPALLRPGRFDRQITV
                     GYPDAKGREEILRVHSRKKPLAPDVDLGVIARSTVGFTGADLENLLNEAALLAARRKK
                     RAITMAEIEEATVKVLVGAEKRSKKQSEKDRKLTAYHEAGHAVVSYFLETQDPVHQIS
                     IIARGMAGGYTMSVPKEDRSYISKSSMIEDIVELLGGRIAEALIIGDISTGASNDIER
                     ATDIARKMVTRYGMSETLGPIAFGTGHDEVFLGKEYGTIRNYSELTASEIDNEINRII
                     TNAYNRGKEILSDNVHKLHLVANALLEFEKISGEDFKRLMDAKNEEEENEVLESMRTA
                     PKGSTGTTLYSYHTKKAKEEELLKQFKDEVTNTEKEAAEPETAKDTEPAPENAAEAEK
                     EDAEDSEEK"
     gene            1440..3632
                     /locus_tag="PLD98_01710"
     CDS             1440..3632
                     /locus_tag="PLD98_01710"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HQA04998.1"
                     /translation="MTGKEMFGKAKWFSPSSDYSQPIFADTFEAPDFDMAELIICGLG
                     FFKAYINGRSVSSDLFVPAWTDYNHVDRYINGELYKDRFRHSINVLKYDVSLHIKPQN
                     NIIAVLVGPGWYVKYNFGKVKLCFKIDFYKDGKLQGSFYSNDTVKWTKSHITESQIIR
                     HEIQDFNVAPEGFIENLTPDSLSDDRWQKAVEIEAPESDYVFQTTPADKVIRTLSPKL
                     VRDFGDIKVYDVGQNISGRVKIDISGLKAGESALIVYSELINEDGEIDFATHSWGKPQ
                     ANEFISDGKAKIAAAEFSWFGFRYFSLTSNAKPIVCDVIHADVPVTSGFMSSSYALNW
                     LYDAFINTQLTNMHAGIPSDCPHREGRGYTGDGQLVSGCGLLLFDSKEFYKKWLRDIA
                     DCQDETTGHVQYTAPFVQSGGGPGGWGGAIAIVPYNYYLRFGDLSVLKEFFPRMLKYF
                     KYLDDHSENDLVVSDEPWAWCLGDWCTPDEIKIPVPFVNNYFYVKTLEIAIKTAKLIG
                     KEEYIPELSEKLNIKKAATVKHYFNESTGDFADNIQGANAFAVDIGLGDERTFQRMAE
                     NYKKLGMYDTGIFGTDIVTRVLFERGQGQLAFDLLTSEKEISFSTQMKKGATTIWEYW
                     THKKSNNHPMFGAVVKYLFDYLLGIRQKAETAGCKDIIISPVFVNGLDFVSGYITLDT
                     GKLEVTISKNEKKADVTVFVPDGIKATLLDGDKALELKAGTNKYQYKL"
     gene            3648..4892
                     /locus_tag="PLD98_01715"
     CDS             3648..4892
                     /locus_tag="PLD98_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQA04999.1"
                     /translation="MGKILVVGGGPAGFMAAISAARYGDEVFLLEKNNRYMKKLLITG
                     KGRCNVTNYQTLLNELIEAVPVNGRFLFSAFSKFMPTDTMDFFEDMGVPLKVERGNRV
                     FPESDKSSDIADALYNKAKKLGVNFLQGRAVKLILEDGELTGVKTENETIKADRVIIA
                     TGGLSYPQTGSTGDGYKLAKEAGHTIVPLKPALVPIETKEDWCRDLMGLTLKNIAISV
                     VDSDTYREVFNDFGEMLFSHFGVSGPVILSASSHMKDAVEGKYKIIIDLKPALNYDKL
                     DNRILRDLAEFANKGMSNALAKLLPKALIPVVLRLSEIPHDLKANQLRKEQRAVLVNT
                     IKNLTVTFKKFRPIEEAIVTSGGVSVKEINPKTMESKLIKGLYFAGEIIDVNAYTGGY
                     NLQIAFSTGYLAGESAAESLRN"
     gene            4905..5585
                     /gene="cmk"
                     /locus_tag="PLD98_01720"
     CDS             4905..5585
                     /gene="cmk"
                     /locus_tag="PLD98_01720"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQA05000.1"
                     /translation="MINIAIDGPSGAGKSTVAKAVAKKLGFIYVDTGALYRAIGVNAL
                     RLGYDTTDREKVVSTLKDVKLDLRYINGEQRVILNGEDVSEAIRLPEASMAASNVSAI
                     PEVRQFLFDLQRSLAAENNCIMDGRDIGTVVLPNADLKLFLTASPEERAMRRHRELIQ
                     RGTPVDYEVLLEEIKQRDYNDSHRAIAPLKPADDAIIVDTSGNTLEESIENVLALIKE
                     KLGIDTEN"
     gene            5586..7679
                     /locus_tag="PLD98_01725"
     CDS             5586..7679
                     /locus_tag="PLD98_01725"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF000907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HQA05001.1"
                     /translation="MIYTAEIKVAKTAGFCFGVDRAVNKVNELLEAGQKVCTLGPLIH
                     NPQFIKSLTEKGVVVAESPDDCPEGYTLVVRTHGITKETADRIKELSISFVDATCPYV
                     SKIHKLAEKHYEKSAVIIVCGDSSHPEVKGIVSHCKDRSYVVSSESQIEDVISDIKKR
                     FGEQFSDIVLLSQTTFSVEEFKKCVKKLKILCTNPIIFDTICSATAERQAEAVSLAKT
                     CDMMVVIGGRNSSNTQKLKDACEKYCPTYLVESYEELKNIDFGKAECIGVTAGASTPA
                     CNIKEVLKYMSDMVNDRDNLEALAAEEIAENSDYISEDEKLFAEALEKMDNDNFNPKV
                     IGTVQRITPTEIHVDIEGRKQTGIVPVEEYSSDPNADPSKELKVGDQLELIIMKTNDV
                     EGTIMLSKRRLDASKHWEAIVDAHKNGTVVTGKVVEVISKGAVAFSKGIRVFIPASLA
                     TASRKDRIEDLLDTEVEFIIIDIDKRRKRAVGSIKDVLRKKKAEAAEKFWATAKVGDV
                     LKGTVKSLTSFAAFVDLGGVDGMIHKSELSWNRVGHPSEVVKEGDEVEVYIKALDSEK
                     KKISLGYRKPEDNPWEILKKQYPVGTVLDVTITGLTTFGAFAQVMPGIEGLIHISQIS
                     NERIAKPQDVLSIGDQVKVMVTSIDAEKKRLSLSMSALLEPPAKEEAEEPEQDEPVAV
                     SIDELLASAENKEAE"
     gene            7794..8525
                     /locus_tag="PLD98_01730"
     CDS             7794..8525
                     /locus_tag="PLD98_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQA05002.1"
                     /translation="MKEKPFCLPDDNYDTLPSPRQQKEKILKENLPFWDKLTAQDLEL
                     IYNHSTLVKFHKGLRINAGDENCVGVIIITKGALSTSLLSDAGREIVLFKLYKGDICV
                     LSSSCIMEPITFDVNIEVDEDMEGLVINSDAYKKLCDSNVYVDCFTYKLVAENFSSVM
                     WAIHQILFMRFDKRLAVFLVDEAARTGSNIINLTHEQIAKYTGSAREVVSRMLKRFAE
                     DNIVNLSRGEIQIVNKRALLDIIDM"
     gene            complement(8567..9184)
                     /locus_tag="PLD98_01735"
     CDS             complement(8567..9184)
                     /locus_tag="PLD98_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HQA05003.1"
                     /translation="MKKNKKRFVVLVLALILSALPLFSLTASAVPHTVIKGESLWLIA
                     KKYQVGLSEIIAANPQIPNPDLIYPKQIIEVPLLDQGILDYEAEVIRLTNLKRTQNGL
                     SALKTNWELSRVARYKSQDMHDRKYFAHQSPTYGSPFEMMKKFNIKYSTAGENIAKGQ
                     KTPAAVVEAWWNSPGHKANILNASFKQIGVGYVADGNYWTQMFIG"
     gene            complement(9618..10775)
                     /locus_tag="PLD98_01740"
     CDS             complement(9618..10775)
                     /locus_tag="PLD98_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05004.1"
                     /translation="MQKARETLKQNLTSIISLAAVLLAAAATIAMPEITRKGVSNGLS
                     ICGSIIIPSLFPFMVISSFLVESGGAEILGKVFKYPAKYLFGLSPAGGTVVLMSMVGG
                     YPIGAKMSGQLYKNGSITKKEAKNLILFAMNSGPAFSIAAVGTAILGSRKAGYLLFIS
                     TGLAMLLMGIYLNLFKLASRKGKELANKTKRKDNELYSDEQILDAQSPVKQDIFTALT
                     NAVVKATSGIISISAWVIFFSAMAEALNEVLKGKEALQFIKYIAEVTAGTNAAASTGN
                     AALIAFIVAFGGLCVHFQVLSDVLQCGVKFSSFLISRLVHGTIAGIIAKILFTVFPIT
                     TAVFGPSQYPKFALYSTSVPASIALLFMCAVLILDTSFQNGITVKHKRLKA"
     gene            10897..11202
                     /locus_tag="PLD98_01745"
     CDS             10897..11202
                     /locus_tag="PLD98_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HQA05005.1"
                     /translation="MAKSRKKGKDKQPKTSLMTKLATELEVPSEILPGVFRIELQETR
                     EATVDGCKGILEYKDERIKLKLSNKTVTFAGLNLELKTFTNERVIISGYIAGIDFSE"
     gene            11219..12412
                     /locus_tag="PLD98_01750"
     CDS             11219..12412
                     /locus_tag="PLD98_01750"
                     /inference="COORDINATES: protein motif:HMM:NF018591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HQA05006.1"
                     /translation="MAVLRILRYIFGYVHFRAYGGFAERFINLCAKDKIPIWDVKYFD
                     GGITAYTMAAAEKTISSAAEKSGMIFEPIEKKGVPYFIKRYRFRFGVAIGLFLTLIFS
                     IILSSMIWYVKVEGNEELTEEEIITELEALGLKPGVFKKGIDVKELQMTMLYKLDELS
                     WISVNINGSTAIVEVRERNKAPEIIDRGQPSNIVAGHDGQIIKLEVYQGDGMVEEGSA
                     VVKGDLLISGVETLKDGKVLFHRARGSAIARTERNIVSITNNGFPAKKRTSQKLKYSV
                     YFFGIIIPLAPSVEADEWYKTNHMLMSDQTIIPVGIIRERFTSYSSGTLKLTNEQLRL
                     VTILDFYKDQKEKLANVKEMKSSKFGIESKSDSCKLTANYILIEEIGVEKFFEVEDNR
                     KNSNN"
     gene            12597..13574
                     /locus_tag="PLD98_01755"
     CDS             12597..13574
                     /locus_tag="PLD98_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQA05007.1"
                     /translation="MFEQTISIDRLDHAVSLFGSFDQHIRTLEKEFAVSVVNRGSELK
                     VTGEPEDVGLAVRVIGGLLTLINKGEHLSDQSVRYVISLVRQGSEDKLSTMTQDCICI
                     TSRGKPVKPKTLGQKKYIEAIKNNTIVFGVGPAGTGKTYLAVAMAVTAFRAKEVNRIV
                     LTRPAVEAGENLGFLPGDLQHKVDPYLRPLYDALFDMLGAENFQKFQERGNIEVAPLA
                     YMRGRTLDDSFIILDEAQNTTREQMKMFLTRLGFNSKIVVTGDITQIDLPEGKRSGLI
                     DVLKILKGIDDIQQVFFTEHDVVRHKLVQDIIRAYEKSEEGKKQHGRKS"
     gene            13558..14061
                     /gene="ybeY"
                     /locus_tag="PLD98_01760"
     CDS             13558..14061
                     /gene="ybeY"
                     /locus_tag="PLD98_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQA05008.1"
                     /translation="MAGKVKVIITDDQKDIRIPTGIRMLIRRCCNAVLKLENFEGGAE
                     ISVRFVNDEEIHRLNKEFRNVDAPTDVLSFPLKNGDDYDINYDTGLKMLGDIVISIER
                     AVDQAKEYNHSLQREIAYLTVHSMLHLLGYDHEGGGLPAVRMREKEETVLTQIGLPRN
                     SSYYMDE"
     gene            14072..14968
                     /gene="era"
                     /locus_tag="PLD98_01765"
     CDS             14072..14968
                     /gene="era"
                     /locus_tag="PLD98_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQA05009.1"
                     /translation="MPKTAFISIIGRPNVGKSSILNALIGQKIAIVTPKPQTTRTRIM
                     GVLTKGETQLVFTDTPGFHRPKNLLGEKMVKAVGETISAVDVCMLVVEPENQIHPAEE
                     ELLSKIKKQEIPAILVINKIDTLKNKAEILATIDLFRNEYDFIAIVPVSALDGDGLDD
                     LLNVLCEQAYESPHFFPDDALTDQPERVIAAEMIREKLLMLLDKEIPHGTAVSIERFS
                     EREDGSGILDIEAEIYCERNTHKGIIIGKNGSKLKEISTLARKDMENFFGCKVFLTTY
                     VKVKEGWRNKGGLIRNFGLDQS"
     gene            14974..15711
                     /gene="recO"
                     /locus_tag="PLD98_01770"
     CDS             14974..15711
                     /gene="recO"
                     /locus_tag="PLD98_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQA05010.1"
                     /translation="MIFTTDAVVIRERQSGEADRLLTLLTRDRGVINAFAKGACRPKN
                     KLHSSTQLFSYASFTLYEGKSISVNEAAQKELFFGLREDIEKLSLAQYFCEVMCLSVP
                     SNTYSDDYLRLLLNSLYFLAEGVLPAKMLKSVFELRTAVIEGYMPELSSCANCGADTG
                     QMYFDIENGSLICSECKVTSKKLKPLTGAVLAAMRHISNASFKDLFRYSLSGESLNAL
                     SDITESYFLYHMPYTFKTLDFYKSIAT"
     gene            15831..16787
                     /locus_tag="PLD98_01775"
     CDS             15831..16787
                     /locus_tag="PLD98_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HQA05011.1"
                     /translation="MAERYYLGADGGGTKTTVRVSDSKGKIVAEVVGGSINFYSEGME
                     RARENMANIIAELKVNNPDIEIYSAFIGMSALYKRATEKECADFFTGVIDAPYAFMDS
                     DLFIALEAMGLEGECAVMICGTGSMIAGRKKDGSIIVKGGYGYILGDEGSGYNLGLAG
                     IKRGVAAFEGAKSPTALVDAIREYYKVEDLHELIDIFYDPPMERSAIADFARVVFKCA
                     ESGDEAAIELVKFEAEEAALTAAALLREFDEPISLGVYGGMFQHHKIYTDFFTDKLNS
                     IIATKEIKLLPMPPVNGAILAALRESGTEITDEILNNLKEAK"
     gene            16787..18457
                     /locus_tag="PLD98_01780"
     CDS             16787..18457
                     /locus_tag="PLD98_01780"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HQA05012.1"
                     /translation="MVFPAPQKIEASKYISCKEEPIINLIAPKDIMEKRTAFGSAFSS
                     SGNVVVTAEVIDSREYSYVNEIAKYTKEKYILNITGDESMIEIKACSSSKRGLWYALN
                     TIEKLVEKNSFPIGQIVDYPLFQMRGLIEGFYGNPWSFSARRELIALASRNNMNTYFY
                     APKDDSYHRRRWRELYPEEELSQLADLVKFTDDVCMDFHYCIAPGLSMCYSSEDDFEY
                     LKKKTEQLYSIGIRKFGLLLDDIPEKLAHEDDAKLYGETVNAHIDLINRYYNYLLELD
                     GSIELTICPTQYHGKGNEYYISKLGQAIPPMVNIFWTGLDICSREITTYEAIRFIDNT
                     KHMPLYWDNYPVNDAEMMNEMHIGPLIGREKDLYKVSRGLIANCMEYAQCTKIPLMTI
                     ADYLWNPEKYDSDKSFMNAIEANIEEEGREHFVIFADHLKTSCLKDNNSYIMEQSLSK
                     AAAMLSGGDFLGAFAEVEAYGEKLFAAAEYLKNKNDGIYAELKRWKDKFIFCAEIFEQ
                     AVEYMNSGDEEVGKKIAEMNKKYNSEATVLTGFAFRAFIELILAGLDI"
     gene            18477..19454
                     /locus_tag="PLD98_01785"
     CDS             18477..19454
                     /locus_tag="PLD98_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HQA05013.1"
                     /translation="MAKIIGESIPNIPWQERPAGSSEIAWRYDGNPIIKRDAIPGSNS
                     IFNSAVVKFKDGFAGVFRVDDTTRKQTLHRGFSKDGINWDIDEKCIEFVCDDPEISEF
                     VYGYDPRVCFIEDRYYVTWCNCYYGPTIGVAYTFDFETFYQLENAYLPFNRNGVMFPR
                     KINGNYFMLSRPSDNGHTPFGDIFISQSPDMCYWGKHRHVMSPKENWEATKIGAGPIP
                     IELDEGWLLIYHGVLTSCNGFVYSMGAAILDKDEPWKVLYRAKPYILNPRTDYECVGD
                     VPNVTFPCAALADADTGRIAIYYGCADTTVGLAFTQVDELVDFIKKNNG"
     gene            complement(19506..19820)
                     /locus_tag="PLD98_01790"
     CDS             complement(19506..19820)
                     /locus_tag="PLD98_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05014.1"
                     /translation="MIGLGKWKARVDHMFFKGDVILEIKDNNGEYDFDIDLNADMELP
                     DFRFYDIVEDGNTLKGKGEVSLLPGKEIEAFLEFDGDKMSGYLKAPLVGKIKIKDGVK
                     VG"
     gene            19998..21479
                     /locus_tag="PLD98_01795"
     CDS             19998..21479
                     /locus_tag="PLD98_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009169793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HQA05015.1"
                     /translation="MSLMELKSGSDIRGTAVGEKNQLTDEVVRKLALAFSVLLSEKTG
                     KETASLSIAIGHDVRISSERIKNAAVAAFTDRGVTVYDCGLSSTPAMFMTTLKLPIDG
                     AVQITASHHPYDKNGLKYFTKEGGLSGKDIERIIKTAESLPETMVKAEGKIIKTDFMK
                     DYAADLRKMIIAGLPGGGDKPLSGLKIVVDAGNGVGGFYAYDVLEPLGADISGSQFLE
                     PDGYFPNHTPNPENADAIKSVSEATVKAKADLGIIFDTDVDRAACVDESGEEINRNRL
                     VALASAIALKDNEGGTIVTDSVTSDGLKKFIENKLKGKHHRFKRGYKNVIDEAIRLNN
                     EGINAPLAIETSGHAAFRENYFLDDGAYLITKIIILMSSLKKEGKTLLSVISDLEEPA
                     EAKEIRIGIKESDFKSYGEKVISDLIALAEKNANWKLADDNYEGVRVSVNGGEGWFLL
                     RLSVHDPLLVLNLESAKEGCIPKYAEEIKTFFEQYPSLDLSKF"
     gene            complement(21550..21747)
                     /locus_tag="PLD98_01800"
     CDS             complement(21550..21747)
                     /locus_tag="PLD98_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HQA05016.1"
                     /translation="MARSGRSSLVPEAREALNRFKMEAAGEVGVTLKQGYNGDLTARQ
                     AGSIGGQMVKKMIQAYEQGLK"
     gene            22139..>22858
                     /locus_tag="PLD98_01805"
     CDS             22139..>22858
                     /locus_tag="PLD98_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HQA05017.1"
                     /translation="MKLKRLIVVCFCIALLMIPLIVSNAAETVFDPSEEFCLIGDANL
                     DGSVNSEDARLILRYVARFSDLTEEQKALADVDGNGTVNAFDARQVLRFVAKLDPQPK
                     HHVVHQEAVAATCVSSGLTEGSYCDICGKVFLKQETIPAHGLHTIAVDEYVAPTCTEP
                     GLSKGYHCLVCNTVIDAQVEIPARGHKFGDWQVVNAATCVADGLEKRTCSVCEFSETR
                     AIPAHGLHTVEIIEAKEPTCTE"
BASE COUNT         6733 a         4871 c         5367 g         5887 t
ORIGIN      
        1 caggctccga tttccttgaa atgtatgtcg gtgtcggtgc tgccagagta agagacctgt
       61 ttaatcaggc aaagaagtca gctccctgca tagtcttcat tgacgaaatc gacgccgtcg
      121 gtcgccacag aggcgcaggt atgggcggcg gacacgatga aagagagcag actttaaacc
      181 agctgcttgt tgaaatggac ggctttgttg agaatgaagg cgtcattgtg cttgctgcca
      241 caaaccgtcc cgacattctc gaccctgctc ttttaagacc tggcagattt gaccgtcaga
      301 ttaccgtcgg ctatcccgac gccaagggca gagaggaaat tttaagggtt cactcccgca
      361 agaagcctct tgctcccgat gttgacttag gcgttatcgc aaggtcaacc gtcggcttta
      421 cgggcgccga ccttgagaat ctccttaacg aagcggctct gcttgcggca aggcgcaaaa
      481 agagagccat caccatggcg gaaattgaag aagcaaccgt caaggtgctt gtaggcgccg
      541 aaaaacgctc aaaaaagcag agcgaaaagg acagaaagct caccgcctac cacgaggcag
      601 gtcacgccgt tgtttcatac ttccttgaaa ctcaggaccc cgttcatcaa atttcaatca
      661 tcgcaagggg catggcaggc ggctatacca tgtcggttcc caaggaggat aggtcttaca
      721 tctccaagag ctcaatgatt gaggatattg tcgaactgct tggcggcaga attgctgagg
      781 ctcttattat cggcgacata tctacaggcg cctccaacga catcgaaaga gcaaccgaca
      841 tcgcccgcaa gatggttaca aggtacggaa tgagcgaaac cctcggtccg attgccttcg
      901 gcacgggtca tgacgaggtt ttcctgggca aagagtacgg cacaatcaga aactactcag
      961 agcttaccgc atctgagatt gataacgaaa ttaacagaat catcaccaat gcttacaaca
     1021 ggggcaagga gattctgtct gacaatgttc acaagctgca ccttgttgcg aacgcccttc
     1081 ttgagtttga gaaaatcagc ggcgaggact ttaagagact catggacgca aagaacgagg
     1141 aagaagaaaa cgaagtgctt gaatccatga gaaccgctcc taagggcagc acgggcacta
     1201 ccctctactc ctatcacaca aagaaggcta aggaagaaga gctcttaaag cagtttaagg
     1261 atgaagtgac aaacactgaa aaagaagccg ccgaacccga aactgctaaa gatacagagc
     1321 cagcgcccga aaacgctgcg gaagcggaga aggaagacgc tgaggacagc gaagaaaaat
     1381 aaggttttaa agcttacagg cgaggaattt cttcgcctgt tttttaaagg gggattttaa
     1441 tgaccggcaa agaaatgttc ggaaaagcaa aatggttctc gccaagctcg gattattctc
     1501 agcccatatt cgcggatacc tttgaggctc ccgattttga catggcggag cttattatat
     1561 gcgggctcgg tttttttaag gcatacataa acggaagaag tgtcagcagc gatttgttcg
     1621 tccccgcatg gaccgactat aaccatgtgg accgatatat taacggcgag ctttataagg
     1681 atagattcag acattcaatt aatgtcctta aatatgatgt ttcgctgcat ataaaacccc
     1741 aaaacaatat aatcgccgta ctcgtaggtc ccggctggta tgtaaagtac aatttcggca
     1801 aggtaaagct ctgctttaaa attgactttt ataaagacgg caagctgcag ggcagctttt
     1861 actcaaacga cactgtaaaa tggaccaaaa gccacataac agaatcacaa attataagac
     1921 acgaaattca ggatttcaat gtcgcccccg agggttttat tgagaatctg actcccgaca
     1981 gcctcagcga tgacagatgg caaaaagccg tagaaataga agctcccgaa tcggattatg
     2041 tattccaaac tacccccgcg gacaaagtta taaggacttt atctcctaaa ctcgtcaggg
     2101 actttggaga tataaaagtc tatgatgtgg gacagaacat ttcgggcagg gtaaagattg
     2161 atatttcggg tcttaaagca ggagagtctg ctttgattgt ttactcagag cttataaatg
     2221 aagatggcga gatagacttt gcgacccaca gctggggcaa acctcaggca aacgaattta
     2281 tttccgacgg aaaggctaag atcgcagctg ccgaattctc ctggttcggc tttagatatt
     2341 tctcccttac atcaaacgcg aagcctattg tctgcgatgt aatacacgcc gatgttcccg
     2401 taacaagcgg ctttatgtca agcagttatg ctctcaactg gctgtatgat gctttcataa
     2461 atactcagct taccaatatg catgctggta tcccctcaga ctgcccccac agagagggca
     2521 ggggatatac cggtgacgga cagcttgttt ccggctgcgg acttctgctg tttgacagca
     2581 aggagttcta taaaaaatgg ctcagggata ttgccgattg tcaggacgaa acaacgggtc
     2641 atgtccagta cactgctccc tttgttcagt cgggcggcgg tcccggcggc tggggcggcg
     2701 caattgccat tgtgccgtat aattattatc tgagattcgg cgatttgagc gtactgaaag
     2761 agtttttccc gagaatgctc aaatatttta agtatctcga tgaccacagc gaaaacgacc
     2821 ttgtggtaag tgacgagccc tgggcatggt gcctcggcga ctggtgcact cccgatgaaa
     2881 ttaaaatccc cgtgcccttt gtgaacaact atttctatgt aaaaaccctt gaaatagcca
     2941 taaagaccgc aaagctcatc ggcaaggaag aatatatccc agagctcagt gaaaagctca
     3001 atattaaaaa ggcggctact gtaaagcatt atttcaatga atccaccggg gattttgcgg
     3061 ataatattca gggcgcgaac gcctttgccg ttgatatcgg gctcggcgat gaaaggacat
     3121 tccaaagaat ggcggagaat tacaaaaagc ttggaatgta cgacacaggc attttcggca
     3181 ccgatattgt aacaagggtt cttttcgaga ggggacaagg tcagctggcg tttgaccttt
     3241 tgacctctga aaaagaaatc tctttcagca ctcaaatgaa aaaaggcgcc acaacaattt
     3301 gggagtactg gactcataaa aagtccaaca atcaccccat gttcggcgcg gttgtaaagt
     3361 accttttcga ttatctcttg ggcattaggc aaaaggcaga aacggcaggc tgcaaagata
     3421 taatcatttc acctgtattt gttaatggtc ttgattttgt cagcggctat ataacactcg
     3481 acacaggtaa gctggaagta acaatcagca aaaacgagaa gaaagccgat gttacggttt
     3541 ttgtgcccga cggcataaag gcgactttgc ttgacggcga taaagcttta gagctgaagg
     3601 caggaacaaa caaatatcag tataaacttt agtctaacgg agaggttatg ggtaaaatac
     3661 ttgttgtggg cggcggacct gcgggcttta tggccgcgat ttccgccgca agatacggtg
     3721 atgaagtttt tcttcttgag aaaaataatc gatatatgaa aaagctcctt attacgggaa
     3781 agggcaggtg caatgtaaca aactatcaaa ccctgctcaa tgagctgatt gaagccgttc
     3841 ccgtgaacgg acgctttctc ttcagcgcat tttcaaaatt tatgcccaca gacacaatgg
     3901 acttttttga agatatgggc gttccgctga aagtggaaag gggaaacagg gttttccccg
     3961 aatcggacaa gtcctcggat attgccgacg cactctataa caaagcaaag aaattgggcg
     4021 taaatttcct tcagggcagg gctgtaaagc tcattttgga agacggcgaa ttaacgggcg
     4081 ttaagaccga aaatgaaaca ataaaagccg atagggttat tatcgcaacc ggcggcttgt
     4141 cttacccgca aacaggctct acgggcgacg gctataagct ggctaaagag gccggtcata
     4201 caatcgtgcc gttaaagccc gcccttgtac ccattgaaac aaaagaggac tggtgcaggg
     4261 atttaatggg gcttacctta aaaaacatcg ccatttcagt tgttgactca gacacctacc
     4321 gcgaggtttt caatgacttt ggcgagatgc tttttagcca tttcggagta tctggtcccg
     4381 ttattctcag cgcaagctct catatgaagg acgctgtaga gggcaaatac aagattatta
     4441 ttgatttaaa gcccgctctg aattatgata agcttgataa cagaatttta cgggatttag
     4501 ccgaatttgc aaataaggga atgtcaaacg cccttgcaaa gctcttgccc aaggctttaa
     4561 ttcccgttgt gttaaggctc tccgagattc cccacgactt aaaggcaaat cagctcagaa
     4621 aagagcagag ggcagtgctt gtaaatacta tcaagaactt gacggttaca tttaaaaagt
     4681 tcaggccaat agaggaagcg attgtgacat cgggcggagt ttcggtcaaa gagattaatc
     4741 ctaaaacgat ggaatctaaa ttaattaagg gtctgtattt tgcgggcgag attatagatg
     4801 tgaacgcata tacgggcgga tataacctcc agattgcctt ttcaacaggc taccttgcgg
     4861 gtgaatccgc cgcggaatca ctgagaaact gaaagaggga taagatgatt aacatagcta
     4921 ttgacggccc ttctggagcg ggcaaaagca ccgttgcaaa ggcggtagct aaaaagcttg
     4981 gatttatcta tgttgacacg ggcgctttgt atagggcaat tggcgttaat gccctgaggc
     5041 tcggttatga cacaaccgac agggaaaagg ttgtaagcac cttaaaagat gtaaagctcg
     5101 atttgcggta tattaacggc gagcagaggg ttatcttaaa cggcgaggat gtttccgagg
     5161 cgataagact gcccgaagca tccatggcgg cgtctaatgt gtccgccatt cccgaggtcc
     5221 gtcagtttct tttcgatttg cagcgcagcc ttgccgccga aaataactgc attatggacg
     5281 gcagggacat aggcacggta gttctcccca atgcggattt aaagctcttt ttaaccgcct
     5341 ctcccgagga aagggcaatg aggcgtcaca gggagttaat ccaaaggggt actccagtcg
     5401 actatgaggt tctccttgag gagataaagc aaagggacta taacgactcc cacagggcga
     5461 ttgctccctt aaagcccgcg gatgacgcta ttattgttga tacatcgggc aatacccttg
     5521 aggagtccat tgaaaatgta ttggcattaa ttaaagaaaa actcggaata gatactgaga
     5581 attaggtgat ttatacggct gagattaagg ttgccaaaac cgcaggattt tgctttggcg
     5641 ttgacagagc ggtaaacaag gtaaatgagc ttttagaggc aggtcagaag gtttgtacct
     5701 tggggccttt gattcataat cctcagttta taaagagcct aacggaaaaa ggcgttgttg
     5761 tggccgaatc tcccgatgat tgccccgagg gctacactct tgttgtcaga acacacggca
     5821 tcacaaaaga aacagcggat agaataaagg agctaagtat cagttttgtc gacgccacct
     5881 gcccctatgt ttccaagatt cataaactag ccgaaaagca ctatgagaaa agcgcggtta
     5941 ttatagtctg cggcgacagc agtcatcccg aggtcaaggg aatagtcagc cactgcaaag
     6001 acagaagtta tgtagtaagc agcgaaagtc agattgaaga tgtaatttcc gacattaaaa
     6061 agaggttcgg agagcaattt tctgacatag ttttgttaag tcaaacaact tttagcgtcg
     6121 aagaattcaa aaaatgtgta aaaaagctga aaatactatg tacaaatcca ataatttttg
     6181 atacaatatg cagtgctact gctgaaagac aagccgaagc tgtcagtttg gctaaaacct
     6241 gtgacatgat ggttgttatc ggcggtcgca acagctccaa cactcaaaag ctaaaagacg
     6301 cttgcgaaaa atattgtcct acttatctgg ttgagtcgta tgaagagctg aaaaacatcg
     6361 actttggcaa ggctgaatgc attggtgtta cggcgggagc ctctactccc gcctgcaata
     6421 taaaggaggt acttaaatac atgtccgaca tggtaaatga ccgcgacaat cttgaagcgc
     6481 tggcagcaga ggagattgct gagaattcgg attacatttc cgaagatgag aagcttttcg
     6541 cagaagctct cgaaaaaatg gataacgata atttcaaccc gaaggtaatc ggtacagttc
     6601 agagaattac tcctaccgaa attcatgttg atatagaagg ccgcaagcag acaggtattg
     6661 tgcctgttga agaatacagc tcagacccga acgccgaccc ctcaaaggag cttaaagtag
     6721 gtgaccagct tgagcttatt atcatgaaga caaacgatgt ggaaggcaca ataatgcttt
     6781 caaagcgccg tcttgacgct tctaagcatt gggaagccat tgtggacgcc cataagaacg
     6841 gaactgttgt taccggcaaa gtggttgaag ttatcagcaa gggtgcagta gcattttcaa
     6901 agggaattcg tgtattcatc cccgcatcac ttgcaacagc ttcaagaaaa gaccgcattg
     6961 aagacctgct tgacactgag gttgaattca taattatcga tatcgacaaa cgccgcaagc
     7021 gcgcagtcgg ctccattaag gatgtactcc gcaagaagaa ggcagaagcc gcagaaaaat
     7081 tctgggcaac cgcaaaagtg ggcgatgttc tcaagggcac cgtcaaatcc cttacatcct
     7141 ttgcggcttt cgttgacctt ggcggcgttg acggcatgat tcataagtcc gagctttcat
     7201 ggaaccgtgt aggacatcct tccgaagttg ttaaagaggg cgacgaggta gaggtttaca
     7261 tcaaagctct tgacagcgaa aagaagaaga tttctctcgg ctacagaaag cccgaggaca
     7321 atccgtggga gattctcaag aagcagtatc ctgtcggcac agttcttgat gtaacaatca
     7381 caggacttac aaccttcggc gcattcgcac aggtaatgcc cggaatagag ggacttatcc
     7441 atatttccca gatttccaat gagcgtatag caaagcccca ggatgttctt tcaatcggcg
     7501 atcaggtcaa agtcatggta acaagcattg acgctgagaa gaagagactc agcctctcca
     7561 tgagcgcact tcttgagccc cctgcaaagg aagaagctga agagcccgag caggatgagc
     7621 ctgtagctgt atcaattgac gaacttcttg catctgccga gaataaagag gcagagtaag
     7681 ttaaatctgt tgaattccgc cgcaactgac ctttttgcag aaggctcttg cggctttttt
     7741 ttattaatgc taaactggaa atacgaaatt ttgttgtttt gtggaggtgc tcaatgaaag
     7801 aaaagccatt ttgcctgccc gatgataatt acgacactct accttcaccc cgtcagcaaa
     7861 aagaaaaaat attaaaagaa aatcttccct tctgggataa gctcaccgcg caggatttgg
     7921 agctgattta caaccactcc acgctggtaa agttccataa gggactgaga attaatgcag
     7981 gcgatgaaaa ctgcgtcgga gtcataataa ttacaaaggg tgctttgagc acatcattgc
     8041 tttctgatgc gggcagggaa attgtgcttt ttaagcttta taagggcgat atttgcgtac
     8101 tttcttcatc gtgcataatg gagcccatta cctttgatgt gaacattgaa gttgatgagg
     8161 atatggaagg tcttgtaata aactcggatg cctataaaaa gctctgcgac agcaatgttt
     8221 atgtagactg ctttacatat aagctggttg cggaaaactt ttcaagcgta atgtgggcga
     8281 ttcatcaaat tctctttatg cgctttgata aaaggcttgc cgttttcctt gttgatgaag
     8341 ccgcaagaac gggctcaaat ataattaatc taacacacga gcaaatcgct aaatatacag
     8401 gcagcgcaag agaagtagtt tcccgaatgc ttaaaagatt tgctgaagac aatatcgtaa
     8461 atctctcaag aggagaaatc caaatcgtca ataaacgggc gctgcttgac attattgata
     8521 tgtaataacc aaaacttagg ggcagctttt atgctgcccc caaattttat cctatgaaca
     8581 tctgtgtcca gtaattgccg tcggcaacat aaccgacgcc gatttgctta aaggatgcgt
     8641 tcaggatatt tgccttgtga ccgggtgagt tccaccaagc ttcaacaacc gcagcaggag
     8701 tcttttgtcc cttcgcgatg ttttcgcccg cggtggagta cttaatgtta aacttcttca
     8761 tcatttcaaa ggggcttccg taagtcgggc tctgatgagc aaagtacttt ctgtcatgca
     8821 tatcctgaga cttgtatctt gcaacccttg acagctccca gtttgtcttc aaggcgctta
     8881 aaccgttctg agttctctta aggttcgtga gtcttataac ctctgcttcg tagtccagaa
     8941 tgccctggtc cagtaaggga acttcgatta tctgcttagg atatataagg tcaggattag
     9001 ggatttgcgg gtttgcggca attatttcgc tgagacctac ctgatatttt tttgctatca
     9061 gccaaagtga ttcgccttta ataactgtat ggggaaccgc gctcgcagtc aagctgaaaa
     9121 gaggcagggc agacagtatc aacgcaagta ccaaaacaac aaatctcttt ttgttctttt
     9181 tcatacttca tccttccagt ttatttaaat tttttagact gtccgtccat gattattttt
     9241 ttcagcaata ttcatattaa gcaatgtaat ttttacgggg aaaattaaat ctaataggaa
     9301 aataaaagaa aaaatctatc tctgaagaga tagatttttt aaatctcaaa atctttttgg
     9361 tcgaatttag gaaggcgcgg aatccgttta ggaattcttt ttaaatggtc atatttaaat
     9421 tttttgcaat atgaatccaa ggtcattaca cccatataag ggcaaagaat catatcggaa
     9481 tctttaaggt ttctgccgtt ttggcagtat gagcagcggg cttcataatt tgaattaaag
     9541 aaatttagct tcatattcgc cacctgaatt tgctttttaa taagtttacc acagtataaa
     9601 ggcggcggca agaattatta tgcttttaat cgcttatgtt taacagttat tccgttttga
     9661 aagcttgtgt caaggattaa aaccgcgcac ataaacaaga gagcgatgct tgcgggcaca
     9721 gaagtggaat aaagcgcaaa tttggggtac tgacttggac caaagactgc ggttgtaatg
     9781 gggaatacgg taaagagaat ttttgcgatt atgcccgcaa tcgtgccatg cacaagtctt
     9841 gatattaaaa atgaggaaaa ctttacgccg cactggagta catcgcttaa aacctgaaaa
     9901 tgaacgcaaa gcccgccaaa tgctacgatg aaagctatca aagccgcgtt gcccgttgat
     9961 gctgcggcat tggtgcccgc agttacctca gcgatatatt taataaactg caaagcctct
    10021 ttgcccttga gtacctcgtt caaagcctct gccatggcgg aaaagaatat gacccacgca
    10081 gaaattgaaa ttattcccga tgttgcttta actacagcat ttgtcagagc tgtaaagata
    10141 tcctgcttta cgggtgattg cgcgtctaaa atttgttcgt cactgtaaag ctcattatct
    10201 ttgcgttttg ttttatttgc aagctctttt cctttacggc ttgcgagttt gaaaaggttt
    10261 aaataaatgc ccatcaaaag catcgcaaga cccgttgaaa tgaatagcaa atatcctgcc
    10321 tttcttgaac ccaaaatcgc agtgccgacg gcagctatgg agaaggcggg ccccgagttc
    10381 atggcgaaaa gaattaaatt ttttgcctct ttttttgtaa tgctgccgtt tttgtaaagc
    10441 tgtccgctca ttttggctcc gatgggatag ccgcccacca tagacatcag aacaaccgtt
    10501 cctccagccg gagaaagtcc gaaaaggtat tttgccggat acttaaatac ctttcccaaa
    10561 atttctgcgc ccccgctttc aaccagaaaa gaagatatga ccataaaggg gaacagcgag
    10621 gggataatta tcgagccgca aatgctaaga ccgttgctca ctccttttct tgtaatctcc
    10681 ggcattgcaa ttgtcgcggc ggcagccagc agcactgccg caagtgatat aatgctcgtc
    10741 agattctgtt ttagcgtttc tcttgctttt tgcatttttg ctttagtttt atcttgtctc
    10801 atcatactac catgtatatg gcttgtttgt actttttata aagacagttt catatatttt
    10861 ttaacataag aaatctttat tccgtgaggt cgtaatatgg ctaaaagcag gaaaaagggc
    10921 aaagacaagc agccgaaaac atctttaatg acaaagctcg ctacagagct tgaagtgcct
    10981 tccgagattc ttcccggagt tttccgcatt gagcttcagg aaacccgaga ggcaacggta
    11041 gacggctgca aaggcatttt agagtataag gacgaacgga taaagcttaa actcagcaac
    11101 aaaacagtga cctttgctgg gctgaattta gagcttaaga cctttacaaa tgaaagagta
    11161 attatcagcg gatatatagc gggaatagat ttttctgaat aaatatggag gaattatctt
    11221 ggcggtatta aggattttaa gatatatatt cggatatgtg catttcagag cttacggcgg
    11281 attcgccgag cgttttatta atctttgcgc aaaggacaaa atccccatct gggatgttaa
    11341 gtatttcgac ggcggaatta ctgcctacac catggcagcg gctgagaaaa ccatttcatc
    11401 cgcggccgaa aaatcgggaa tgatattcga gcccatagaa aaaaagggtg ttccctactt
    11461 tattaagcgc tacagattca ggttcggcgt tgcaatcggt ctttttctga ccttaatttt
    11521 ttcaattata ctatcatcaa tgatttggta tgtaaaagtc gagggcaacg aggaacttac
    11581 cgaagaagaa ataataaccg agcttgaggc tttgggtcta aagccaggag tttttaaaaa
    11641 gggcattgat gtaaaagagc tgcaaatgac aatgctttat aagctcgacg agctttcgtg
    11701 gatttctgta aatataaacg gcagcacagc catagtggag gttagggaga gaaacaaggc
    11761 acccgaaata attgacagag ggcagccctc caatattgtg gcgggccatg acggtcagat
    11821 tatcaaactg gaggtttatc agggcgacgg catggtagaa gaaggctcag ccgtcgtaaa
    11881 gggcgatttg ctgatttccg gcgttgaaac ccttaaagac ggcaaagttc ttttccacag
    11941 agcgagaggc agcgccatag ccagaaccga aaggaatatt gtttcaataa ccaacaacgg
    12001 ctttcccgcc aaaaaacgca catcgcaaaa acttaaatac tccgtatatt tcttcggaat
    12061 catcattccg cttgcgccgt ctgtggaggc ggacgaatgg tacaaaacaa atcatatgct
    12121 gatgtcagat caaacaatca tacccgtggg aataattaga gaaagattta catcttattc
    12181 aagcggcact ttgaaactta caaacgagca gcttcgcctt gtaaccattc tggattttta
    12241 caaggaccaa aaagaaaagc tcgcaaatgt caaggaaatg aaaagctcaa agttcggtat
    12301 cgagtcgaaa agcgattcct gcaagctgac agcaaattac attctaattg aagaaattgg
    12361 tgttgaaaaa ttttttgaag tggaggataa tcgaaaaaac agtaacaatt aaataaaaat
    12421 ttctcttaga tttcatatat tttgaccgct tttcttgata atgcgccggt tttatgctat
    12481 cataacttcg tgttttgtag tcttttgatg taatatttgc ataatcttga cgcatactaa
    12541 aaggcggaaa tctccttaga gatttcggca ttatatttga ttaaagggaa gatttaatgt
    12601 tcgaacagac aattagtatt gacaggcttg accatgccgt cagccttttc ggcagctttg
    12661 accagcatat cagaactctt gaaaaggagt tcgcggtttc agtcgtaaac cgcggctcag
    12721 agcttaaagt aacaggcgag cccgaagatg ttgggcttgc tgtcagagtt atcggcgggc
    12781 ttttgacctt aatcaacaag ggcgagcacc tttctgacca gagcgtaaga tatgttattt
    12841 cccttgtaag gcaaggctca gaggacaagc tgtccacaat gactcaggac tgcatctgca
    12901 ttacttccag aggcaagcct gtaaaaccca agaccttagg acagaagaag tatattgagg
    12961 ctataaagaa taacacaata gttttcggag tcgggcccgc aggtacgggc aagacttatc
    13021 ttgccgttgc catggcggta accgctttca gggctaaaga ggtcaacaga attgtgctta
    13081 ctcgccccgc ggttgaggca ggcgagaatc tcggattttt gcccggcgac cttcagcaca
    13141 aggttgaccc ctatctgcgc cccttgtatg acgcgctttt tgacatgctc ggcgcggaga
    13201 atttccaaaa attccaggag aggggaaata tagaggttgc gcctcttgca tacatgcgcg
    13261 gcagaactct tgacgacagt tttataatac ttgatgaagc gcaaaacacc acaagggaac
    13321 agatgaagat gttccttacc cgtctcggat ttaactccaa aattgttgtt acaggcgata
    13381 ttacacagat agaccttccg gagggcaagc gctccggtct tatcgatgtc ttgaaaatat
    13441 tgaagggtat tgatgatatt cagcaggttt tctttaccga acacgatgtt gtaagacaca
    13501 aactggttca ggatattatc agggcctacg agaaaagcga ggagggcaaa aaacaacatg
    13561 gccggaaaag ttaaagtaat tataactgat gaccagaagg atataagaat acctaccggc
    13621 attagaatgc ttataaggcg ttgctgcaac gccgttctaa agctggaaaa ctttgaaggc
    13681 ggtgctgaaa tcagcgtccg ctttgtaaat gatgaggaga tacataggct aaataaggaa
    13741 ttccgcaatg tggacgcccc gaccgatgtt ctctcattcc cgttaaagaa cggcgacgac
    13801 tacgatataa actacgacac agggcttaaa atgctcggcg atatagtaat ttcaatcgaa
    13861 agagcggtag atcaggcaaa ggagtataac cattctctgc agagagaaat tgcttatctt
    13921 accgttcact caatgcttca tcttctcggc tacgaccatg aaggcggcgg acttcccgct
    13981 gtccgcatga gggagaagga agaaaccgtt cttactcaga ttggtctgcc cagaaattcg
    14041 agctactaca tggatgaata aaggagagaa aatgcctaag accgcattta tttcaataat
    14101 aggacgcccc aatgtgggca agtcctccat acttaacgcg cttatcggac aaaaaattgc
    14161 aatagtgaca cccaagcccc aaaccacaag aacgagaatc atgggtgttc tgactaaggg
    14221 cgagacccag cttgtcttta ccgacactcc gggctttcat cgcccgaaaa atttgttggg
    14281 cgaaaaaatg gtaaaggcag tgggcgagac tatttccgcc gttgatgtct gcatgcttgt
    14341 cgtggagccc gagaatcaga ttcaccccgc tgaggaggaa ctgctttcca aaatcaagaa
    14401 gcaggagatt cccgcaattc ttgttattaa caaaattgat actcttaaaa acaaggctga
    14461 gattctcgca accatcgacc ttttcagaaa tgagtacgac tttatcgcga tagttcccgt
    14521 cagcgccctt gacggcgacg gacttgatga tttgctgaat gtcctttgcg agcaggcata
    14581 cgagtcgcca catttcttcc ccgacgatgc ccttaccgac cagcccgaaa gggtaatcgc
    14641 cgcggagatg ataagagaaa agcttcttat gctccttgat aaggaaatcc ctcacggcac
    14701 agctgtcagt attgagcggt tcagcgaaag ggaggacggc agcggaatac ttgacataga
    14761 agcggaaatc tactgcgagc gcaatacaca taagggaatc atcatcggta aaaacggctc
    14821 aaagctaaag gaaatctcca cccttgccag aaaggatatg gagaatttct tcggctgcaa
    14881 ggtcttttta acaacatatg taaaggtcaa agagggctgg cgaaacaaag gcggtcttat
    14941 ccgcaacttt ggtcttgatc agagctgaag gatatgatat tcaccacaga tgcggttgtt
    15001 atcagagagc gtcaatcggg cgaggcggac aggcttttaa ctctgcttac aagggatagg
    15061 ggagtcataa acgccttcgc caagggcgcg tgccgcccaa agaataagct gcacagttca
    15121 acgcagcttt tttcttatgc ttcttttacc ctatatgaag gaaaaagcat ttccgtaaac
    15181 gaggcggcgc agaaggagct gtttttcggg ctcagagaag atattgaaaa gctgtcacta
    15241 gcccagtatt tttgcgaggt aatgtgcctt tcggttccgt ccaatacata ttcggacgat
    15301 tacttaaggc tccttttaaa cagcctgtat tttctggcgg aaggcgtttt gcccgcaaag
    15361 atgcttaaat cagtgtttga gctgaggact gccgtaattg aaggctatat gcccgagctt
    15421 tcttcttgcg caaattgcgg agcggatacg ggtcaaatgt attttgatat tgaaaacggc
    15481 agcttaatct gttcagaatg caaagtgact tcaaagaagc taaagcctct caccggcgcg
    15541 gttcttgctg ccatgaggca catctcgaat gcctctttta aggatttatt taggtattcc
    15601 ctcagcggcg aaagtctcaa cgctctttcc gatattaccg aatcatattt tctctaccac
    15661 atgccctata cctttaagac acttgatttt tataagtcaa tagctacata aaaagctgat
    15721 gagaaatgtc gcatcggctt aattttgtat ttctgaattg tattgattaa atcatcggca
    15781 tattatacaa taatgtataa tattggaatt gatttttagg ggcggataat atggctgaaa
    15841 gatattatct cggcgctgac ggcggcggca caaagacaac ggtcagagtt tcggacagca
    15901 aaggcaagat agtagccgag gttgtcggcg gttccatcaa tttctattca gagggaatgg
    15961 aaagagccag agaaaacatg gcgaatatta ttgccgagct taaagtcaat aatcccgaca
    16021 ttgaaatata ttcggcattt ataggaatgt ctgccctata caaaagagcc accgaaaagg
    16081 aatgcgccga ctttttcaca ggcgtaatcg acgcgcccta tgcatttatg gacagcgacc
    16141 tatttatagc tcttgaggct atgggcttag agggcgagtg cgccgtaatg atttgcggaa
    16201 cgggctccat gattgcgggc agaaaaaaag acggctcaat cattgtaaaa ggcggctatg
    16261 gatacatact cggcgatgaa ggtagcggat acaatttagg tcttgcggga attaaaaggg
    16321 gagtcgccgc gtttgaaggc gcgaaatccc ccaccgcttt ggttgacgcg attcgagaat
    16381 actataaggt cgaggacctg cacgagctta ttgatatttt ctacgacccg cccatggaga
    16441 gaagcgccat agccgacttt gcgagagtag tctttaaatg cgccgaaagc ggcgacgagg
    16501 cggctattga acttgtaaaa ttcgaggcgg aggaggcggc tttaaccgcg gcggcccttt
    16561 taagggaatt cgatgagcca ataagtctcg gagtctacgg cggcatgttc cagcatcata
    16621 agatttacac cgatttcttt actgataagc taaactcaat aatcgcaaca aaggaaataa
    16681 agctattgcc catgcccccc gtcaacggtg ccatacttgc ggcgttaaga gaaagcggta
    16741 cagagattac cgatgaaata ttaaataatt taaaggaggc taaataatgg ttttccccgc
    16801 accgcaaaaa attgaggcgt caaaatacat ttcatgcaaa gaagagccta ttataaatct
    16861 tatagcccct aaagacataa tggaaaagag aacagccttc ggttctgcct tttcaagctc
    16921 cggcaatgtg gttgtaaccg cggaggttat agactcgaga gaatactctt atgtgaacga
    16981 gattgctaaa tacacaaagg aaaaatacat cctcaacatc acaggcgatg aaagcatgat
    17041 agaaatcaaa gcctgttctt cttcaaaaag agggctttgg tacgcgctga ataccattga
    17101 aaagcttgtg gagaaaaaca gcttccccat cggtcaaatc gtggattacc cgcttttcca
    17161 aatgcgcggt ctgattgagg gattctacgg caatccctgg agcttcagcg caaggcgtga
    17221 gcttatagca cttgcctcca gaaacaatat gaatacctac ttctatgctc ccaaggatga
    17281 ctcataccac cgcagacgct ggagagagct ttatccggag gaggagctgt cgcagctagc
    17341 cgaccttgta aagtttaccg acgatgtttg catggatttt cattactgca tagctcccgg
    17401 tctttcaatg tgctattcat cagaggacga tttcgagtac cttaaaaaga agactgaaca
    17461 gctttactct atcggcatca ggaaattcgg acttctcctt gacgatatac cggaaaagct
    17521 tgcgcatgaa gatgacgcaa agctttacgg cgaaaccgta aacgcccata ttgaccttat
    17581 taacaggtat tataactacc ttcttgagct tgacggcagc attgagctta caatttgccc
    17641 gacgcagtat cacggcaagg gcaacgaata ctatatctcc aagctgggtc aggcaatccc
    17701 gccgatggtc aatatcttct ggacaggtct cgacatctgc tcaagggaga ttacaaccta
    17761 cgaggcaata aggtttattg acaatacaaa gcatatgcct ttgtactggg ataactatcc
    17821 cgtcaacgac gcggaaatga tgaacgaaat gcacataggt ccgctgattg gcagagaaaa
    17881 ggacctttac aaggtatcaa ggggtcttat cgcaaactgc atggaatatg cccagtgcac
    17941 caaaataccc ctcatgacta ttgccgatta cctctggaat cccgaaaaat acgactccga
    18001 caaatccttt atgaacgcta ttgaggcaaa tatcgaggaa gagggcaggg agcatttcgt
    18061 aatctttgcc gaccatctta aaacctcttg ccttaaagac aacaattctt atattatgga
    18121 gcagtcgctt tctaaagccg ccgcaatgct gtcgggcgga gattttctgg gagctttcgc
    18181 ggaggtagag gcttacggcg aaaaactctt tgccgcggca gagtatttaa agaataagaa
    18241 tgatggcata tacgctgagc ttaaacgctg gaaagataaa ttcattttct gcgccgaaat
    18301 atttgagcag gcggtcgaat acatgaattc cggcgatgag gaagtcggaa agaagattgc
    18361 ggagatgaac aaaaaataca actcggaagc aaccgttctt acgggctttg ctttcagagc
    18421 atttatagag cttatacttg caggactgga tatctaaatt tgaggaggac agtttaatgg
    18481 caaaaattat aggagagtca atacccaata ttccgtggca ggaaagaccc gccggcagca
    18541 gcgaaattgc atggagatat gacggaaatc caattataaa gcgtgacgcg attcccggct
    18601 cgaacagcat cttcaacagc gccgttgtaa agtttaaaga cggctttgca ggcgttttca
    18661 gagttgacga tacaacccgc aagcagactc tgcacagagg cttcagcaag gacggcatca
    18721 actgggatat tgacgagaag tgcattgagt ttgtctgcga cgatcctgag atttcagaat
    18781 ttgtttacgg ctacgacccg agagtttgct ttatagagga cagatattat gtaacatggt
    18841 gcaactgcta ttacggtccc acaatcggcg ttgcgtatac ctttgatttc gaaacctttt
    18901 atcagcttga gaacgcatat ctgcccttta accgcaacgg cgtaatgttc ccgaggaaaa
    18961 taaacggcaa ttactttatg ctcagtcgtc ccagcgacaa cggtcacaca ccttttggcg
    19021 acatcttcat tagccaaagc cccgatatgt gctattgggg taagcacagg catgttatgt
    19081 cgccgaaaga aaactgggag gcaaccaaaa tcggcgcggg tcccattccc attgagcttg
    19141 acgaaggctg gctgctgatt taccatggcg ttcttacctc ctgcaacggc tttgtttaca
    19201 gcatgggcgc cgcaatcctt gataaggacg agccgtggaa ggtattatat cgcgcaaagc
    19261 cctatatctt aaatcccaga accgactatg aatgtgttgg cgatgtgccc aatgtaacct
    19321 tcccttgcgc cgctcttgcg gacgctgata cgggcagaat agctatttat tacggctgtg
    19381 ctgacacaac cgttggcctt gcctttacac aggttgacga gcttgttgac ttcattaaga
    19441 agaataacgg ataataaaaa aagcagaggc atttttgcct ctgctttgat aagtacttaa
    19501 attaattagc cgacttttac gccgtcctta atcttgattt ttccgaccag cggagctttc
    19561 agatagccgg acatcttgtc gccgtcgaac tcaaggaatg cttcgatttc cttgccgggc
    19621 aggagagaaa cctcaccttt gcctttaagc gtatttccat cttcaactat gtcatagaag
    19681 cggaaatccg gaagttccat gtctgcgttg aggtcaatgt cgaaatcata ctcgccgttg
    19741 ttgtccttaa tctcaagtat gacatcgcct ttgaagaaca tgtgatcgac acgagctttc
    19801 catttgccta aacctatcat tttactatct ccttttttac agccgaagct ggaatagcca
    19861 atactttggt tggacatata ttaccattat ttagtaagag agtcaagcgg gatagaaatc
    19921 aaatttagca gtttataaga tataaactga tttttattaa tacttttgtt taatatgctt
    19981 aatgtaagag gtggaatatg tcgctgatgg aattaaagag cggttccgat atcagaggta
    20041 ccgcagtggg agaaaagaat caattaaccg atgaggttgt aagaaaacta gccctggcat
    20101 tctctgttct tttgtccgaa aagactggaa aagaaaccgc atctcttagc atagcgatag
    20161 gtcatgatgt aagaatctca tccgaaagga taaagaacgc cgcggtcgca gcttttaccg
    20221 ataggggagt caccgtttat gactgcggtc tttcatccac tcccgccatg tttatgacga
    20281 ctttaaagct gcccattgac ggagcggtgc agattacagc aagccaccac ccctatgaca
    20341 aaaacggtct taaatacttt acaaaagaag gcggactttc gggaaaggat attgaaagaa
    20401 taataaaaac cgctgaaagt ttgcccgaaa ctatggtaaa ggcggaaggc aaaatcataa
    20461 aaaccgattt tatgaaggat tatgccgcag accttagaaa gatgattata gctggtctgc
    20521 cgggcggagg agataagccg ctgagcggtc ttaaaatcgt tgttgacgcc ggcaacggag
    20581 tgggcggctt ttatgcctat gatgtgctgg agcctcttgg cgcggatatt tcgggcagtc
    20641 agttcttaga gcccgacgga tatttcccga accatactcc caaccccgaa aacgccgacg
    20701 cgataaaaag cgtcagcgaa gcgactgtta aggcaaaggc tgatttgggc ataatttttg
    20761 acacggatgt tgacagagcg gcatgtgtag acgaaagcgg tgaggagatt aacagaaacc
    20821 gccttgttgc tcttgcctct gcaattgctc tgaaagacaa cgaaggcggc acgattgtaa
    20881 ccgacagcgt aacctcagac ggcctaaaga aatttattga gaataagctg aagggaaagc
    20941 accacagatt caaaaggggc tacaaaaatg taattgatga agcgataagg cttaacaatg
    21001 aaggcataaa cgcgcccctt gcgattgaaa cctcaggtca tgccgcattc agagaaaact
    21061 atttccttga cgacggcgcg tatctaatta caaaaatcat tatccttatg tcctcgctaa
    21121 aaaaggaagg aaagacttta cttagcgtca tctccgacct tgaagagccc gctgaggcaa
    21181 aggaaataag gataggaatc aaagaatctg actttaaatc ctatggcgaa aaggtaattt
    21241 ctgaccttat tgccctcgct gagaaaaacg caaactggaa attggctgac gataattacg
    21301 agggtgtaag agtttccgta aacggcggag aaggctggtt cctcttgaga ctcagcgtcc
    21361 atgacccgct gcttgtatta aatcttgaaa gcgcaaaaga gggctgtatt ccgaaatatg
    21421 cagaagaaat aaagacattc tttgagcaat atccgtcatt ggatttaagt aagttctaag
    21481 ttttgaaact aaaccgacgc ctccctatta agggaggcgt ctttgttatt tccggtttat
    21541 cggaaatcct tatttaaggc cctgttcgta agcctgaatc atctttttga ccatctggcc
    21601 accaatggag ccagcctgtc ttgctgtaag gtcgccgttg taaccctgct taagagttac
    21661 gccaacttcg ccggcagctt ccatcttgaa acgattgaga gcttcacgag cttcaggtac
    21721 taaacttgat ctaccgcttc ttgccatcgt attacaactc ctttaaatct attgcgtttg
    21781 caatcatatt attgaccctg attctttcaa atatttctga aaatttttgg cttgattttt
    21841 agaactgttt tataatcagc cgtagatttc tgtatgcggc ttataaactc aattttattg
    21901 agcttcgtaa acattattaa ttttttttgg cttagtattc atttcgagaa aaattgatgc
    21961 cttggaaaat atgaataaga tgtaaataat tggtgaaatc attgaaagta taaatgtaaa
    22021 atgttactat aatttctaaa gaatggtaat aaatggccga ctattcggga tttttatcag
    22081 ttctttgtaa acaattagca ttaagctaat aaaaataaac aggtgtagga ggctatttat
    22141 gaaactcaaa aggttaattg ttgtctgttt ctgtattgcg ctgcttatga taccgctcat
    22201 tgtatcaaac gcagctgaaa cggtatttga cccgtccgaa gagttttgct taatcggtga
    22261 tgccaatctg gacggaagcg taaattccga agatgcccgc ctcatattaa ggtatgtggc
    22321 tcggttctcc gacctgactg aagagcagaa ggctcttgcg gatgttgacg gaaacggaac
    22381 agtaaatgca tttgatgctc gtcaggtttt aagatttgtc gcaaagcttg atccgcagcc
    22441 taaacatcat gttgttcatc aggaagccgt agcagccacc tgcgtaagct ccggtcttac
    22501 cgagggctct tactgcgata tttgcggtaa agtattctta aagcaggaga ccattccggc
    22561 tcacggttta catacaatcg cagttgatga gtatgttgct cccacctgca cagagccagg
    22621 actcagcaaa ggctatcatt gcttggtctg caacaccgtt attgacgcac aggttgaaat
    22681 tcccgcccgc ggtcacaagt ttggcgactg gcaggtagtc aacgccgcta catgcgtcgc
    22741 tgacgggctt gagaagagga cctgctctgt ttgtgagttt tccgaaaccc gtgcaattcc
    22801 cgctcatggc ctgcatactg ttgaaatcat tgaagccaaa gagccgacct gcacagag
//
LOCUS       DAONVQ010000013        22839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_1597_length_22839_cov_5.246269, whole genome shotgun sequence.
ACCESSION   DAONVQ010000013 DAONVQ010000000
VERSION     DAONVQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 22839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22839
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1597_length_22839_cov_5.246269"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PLD98_01810"
     CDS             complement(<1..311)
                     /locus_tag="PLD98_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HQA05018.1"
                     /translation="MNKIELIVSSEYEGERIDKFLALTQPELSRSYIQKLIDDGKVSV
                     NNKLISKCVKVKSGDCISLDVPEPQELELSPQDIPLDIIYEDEHLLVVNKPKGMVVHP
                     AA"
     gene            complement(308..943)
                     /gene="lspA"
                     /locus_tag="PLD98_01815"
     CDS             complement(308..943)
                     /gene="lspA"
                     /locus_tag="PLD98_01815"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQA05019.1"
                     /translation="MQKNKSRLKALLTYLPIIIVLVAIDLIIKYFAKTYLRPIDTIPF
                     IDGILHFTYVHNPGAAFGILRGSSQFLIYFTGVILLGLIIYLVSAKIKSKIEIVSLIL
                     IISGGLGNLVDRALNGHVVDYIQALPAFIDFPVFNFADCLVTVGSFLLIGFLLYDIFK
                     EFKNSKKELPHAESAVSEENALPAETGEISEKADESEESESVSEKTSDETA"
     gene            complement(968..3322)
                     /locus_tag="PLD98_01820"
     CDS             complement(968..3322)
                     /locus_tag="PLD98_01820"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HQA05020.1"
                     /translation="MLTPDKIKSQSFNLIDGQAYSKSEVDAFLEEVSRSYAQMFQENG
                     ELLRKISILATRIEEYRREEDIIKKSLAAAQRSAEKLMADARASISTTLDQARREASL
                     IKIDAQNRAKEIIKSAEESANEKLSQAEETARQLLNAAADKAKEIESSSLASIKEKEE
                     LILQNSTEAQEIRAKAVEEAERIIEEAKKSAENLLETAKAKAEELSRIERENAEKEIA
                     ALKDNAQTYIESARNKSDALLASVLEKSENMEAEARAKAEEIINNANAEAERILSEAN
                     AQAESVVSEANAQAESVISEANAKAESILNEAAAKAESIVAEAEAKAEELKHTTETAA
                     EDLMASTKDMAAELLVSAKNESDSITMAAKMRLEDILPKLQHMEETYNKIKGNCKVLS
                     EAMKLSMDEHLKNIAPEYEGLLNAEGEHYEVPVIEDIDSVDFTDKTADDILMYFGFKA
                     FDSKAVSDEITALNEESEEGVKISDIEEDAESETAEAKEAPETEAAEEAAEVEESAEA
                     HESDDDWDDLGAAEEISVDTSEEVEAIEETEPEAFEIDEEAVSESLDAEVLVNDEEAV
                     AETIESVDEEDDWQDISGFDLDTSAIVEETEIVESEIEEPQSEVTIVETSEEKEGFEI
                     DDSFDDDWDDLSVDSAESKQAEIEVETIITEAQEETEESEQPTEESYFDNTEDLSESY
                     VQIFNKIRDMKKSAVASLDLENEAIVDAEEAVEDEDYDLGFELDTSIFDDDDLDLDTE
                     DDSWDDLSDAKPSELFAEPDTAEEDVEEKTENIKGFEIDFSIFE"
     gene            complement(3372..3854)
                     /locus_tag="PLD98_01825"
     CDS             complement(3372..3854)
                     /locus_tag="PLD98_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HQA05021.1"
                     /translation="MGLVDKLKKIVNIDEDEYYDDVESNDLLVYSPEEEEEPQPRKSI
                     LRAESKPATSVKSGSNKIVDIHTTAKLQVVLTKPERFEDGRAIADNLNEKRTVVLNLE
                     SASRDVARRLVDFLSGVAYANGGQLKRIANSTFIITPYNVDVMGDLLDELESNGVFFI
                     "
     gene            complement(3868..4599)
                     /locus_tag="PLD98_01830"
     CDS             complement(3868..4599)
                     /locus_tag="PLD98_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQA05022.1"
                     /translation="MQERSFLDERLRIIEENYKVIKANIEKAALSVNRNPDEIRLMAV
                     TKTVEPIYIKHAINLGIDLIGENRVQEYLSKEEEIMKNEKKPEVHIIGHLQTNKVSKI
                     IDKVDMIESLDSVKLAKEISKRAVKAGVTMPVLVEVNIGREESKTGFDPDEVIDGIYE
                     IAELEGIKIKGLMCIPPVCDSEKEAMAYFDAISKLFIDIKDKKIDNVDMQILSAGMSQ
                     DYEAAIKCGSNLVRIGSALFGARIY"
     gene            complement(4577..5845)
                     /locus_tag="PLD98_01835"
     CDS             complement(4577..5845)
                     /locus_tag="PLD98_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQA05023.1"
                     /translation="MNRARRKPKSKVVKINFINSSVFKLAGIGAAALILIIYAAAQIY
                     KIYSVPVKTTTALTKTVYKSAVVDIFVIRDEKYIVYDANGSVVPLVKDGTRVTGGDNV
                     AAIFSNDADAKSFIEERQIKNEIAYYEALSKSDINFQSDLTQLNSNIKQSVLNYITAA
                     ESGKLSDLKAQIDRVRETITARQRATGEQVDYSQKLSELKSRLSALSSSRHDTKFVSA
                     TGPGYYVSTADGYENAVPFEKALELSCEEIEELIKKEPEQVPSNVRGKLISGYDFYIA
                     AVLKAELAADLKVGSTVSINFPSSSVGEIKVKVAKISETKDGKTPVIFTSRQMSEELS
                     QLRIERAEIRLEKHEGYAVPNSAIRIVDGEKGVYILRGNLVTFRKINITYSDDDISIA
                     TTPENPEGHLRLYDEIIVEGKNLYAGKVIS"
     gene            complement(5885..6841)
                     /gene="miaA"
                     /locus_tag="PLD98_01840"
     CDS             complement(5885..6841)
                     /gene="miaA"
                     /locus_tag="PLD98_01840"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQA05024.1"
                     /translation="MNLIPVIAVVGPTASGKTALAIELAKALDGEIVSADSMQVYKGM
                     DIATAKPTAEERQGIPHYMMDLVDTSVRYSAALYAEKASEYIADINKRGKVPIVAGGT
                     GLYVDTLLKPHIYTLKEHDMTVRKALEEREKEEGIEALFEELKRVDKETADKLHINNR
                     GRIIRALEVYYLTGRPMSEKQETDEGSPYNVLYLGLDFKNRALLYDRINRRVDIMLEK
                     GLLEEAARYKELSDATTACRAIGHKELLPYINGEMTLEEAVDNLKRETRRYAKRQLTW
                     FRKNKEINWFYVDCHDSFDSVAADAIKMSKDFLVSCKSSQNS"
     gene            complement(6854..8866)
                     /gene="mutL"
                     /locus_tag="PLD98_01845"
     CDS             complement(6854..8866)
                     /gene="mutL"
                     /locus_tag="PLD98_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQA05025.1"
                     /translation="MPKINVLPKSVAELIAAGEVVEKPASVVKELLENAIDSGADKIT
                     VEINSGGIKYIRVTDNGCGIDREDVRTAFLSHATSKIKESTDLNSIFTLGFRGEALAS
                     IAAVSRVNMLTRTADSPVGTAYVIEGGQEVSIDDAGCPVGTTIIVRDLFYNTPARMKF
                     LKKDATEAGYVQDICLKIALSHPHISFRLIKDGKQVLFTPGDGKLISAIHSALGKEFA
                     DTLIPLSYSFEGLSAEGYISLPSFSRVNRNQQYFYINGRFVKIPVGASALDTAYKNSI
                     MVGKFPACVINISIPPETVDVNVHPAKTEVRFSDEKRIFSLIFHAAQSSLAKFDAPSS
                     VALSGDIAAKNKNAYKANFINESKAEEGEDLFSRAAKEKTETLAKMAEIEKRIEEKNP
                     EDSLDKTLRLRTPTIALDYKGYNNPDEFLLKSNTKAKIDSNKENLRFEISEDITDEPL
                     IDLTQKFESASKQVETKNENGPAPEKKAEIKDTPVIIGEAFNTYIIAEMKGKLLLIDK
                     HAAHERMIYNKLKENLFGYSSQLLMSPVKIDLGPREYNAVIDNLELLKKAGFDVSDFG
                     DGSVLVRSCPVDLLSADIRELFEEIADNLASSPNMISSEKIDKIYHSAACKSAIKSGK
                     SISKYEMAEIVDRVLNDNNIRYCPHGRPVMIELTKNELEKLFGRIQ"
     gene            complement(8896..11499)
                     /gene="mutS"
                     /locus_tag="PLD98_01850"
     CDS             complement(8896..11499)
                     /gene="mutS"
                     /locus_tag="PLD98_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQA05026.1"
                     /translation="MADFSPMMTQYLKIKEQYKDALLFFRLGDFYEMFFDDAVTASRE
                     LELVLTGRDCGQDERAPMCGVPFHSAESYIARLVAKGYKVAICEQVEDPATAKGIVRR
                     EVVRVITPGTVTESSMLDEGKNNYIGCIFVENNAAGVAFADISTGDLQAAELQGKFVD
                     NLVINEIARFTPAELIANRESSKRKTIFDFLKNKITLAVDTFDDERFDFEASSKIIKE
                     HFTKLDDEIFEKKMLISAVGALMAYLYETQKNNVSNIEEIKVYNDSKYMALDENARRT
                     LELTQSERRREVKGSLLWVLDSTKTAMGKRMLRQWVENPLVTLPEISMRLNAVDELYS
                     DTIRLMSIKDLLSGISDIERIITRIVYETANARELKVLSNTIKALPELKAALKGFSSK
                     MLNEIERDIDLLSDVYELIETAIIDDPPFTVREGGLIKDGFNAELDELRSIVSGGKSF
                     LVKLEEEEQQKTGIKKLKIGYNRVFGYYIEVLNSYKELVPEHYIRKQTLSNCERYITP
                     ELKELESKVLGSQERIAKLEYEIFAAIRAKTADQQYRIRRTASAIAKLDSLCSLAYAA
                     SQNGYTRPIVDLSDRLEIKNGRHPVVEKFTDEPFVPNDALLDCEDNRTVIITGPNMAG
                     KSTYMRQIALITIMAQIGSFVPAESAVIGIVDGIFTRIGASDDLSMGQSTFMSEMSEV
                     AFIIKNATRKSLIILDEVGRGTSTYDGMSIARALLEYITDKKNIGARTLFATHYHELA
                     QLEDEISGVKNYYITAKKRGDDIIFLRRIVRGASDGSYGIEVARLAGVPKKVADRAKE
                     ILDDIESNKGHYHYVPKSVQSDSNQLSLGNTFADNANLAIIDEIKNLDINVLTPIEAM
                     TILHGIIKRLN"
     gene            complement(11600..12013)
                     /locus_tag="PLD98_01855"
     CDS             complement(11600..12013)
                     /locus_tag="PLD98_01855"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HQA05027.1"
                     /translation="MDLIKLTRQLGAAIQQDERYLAFQAARKANEEDEGLNDLIGKLN
                     LVQLSYQHEAEQPEPDENKLAQYDDEFRSLYSEIMQNPNMINYENTRRDLDELMNYIM
                     QILAMCVNGEDPETCEPQMNEGAGCHGSCSSCQGC"
     gene            complement(12089..13483)
                     /gene="miaB"
                     /locus_tag="PLD98_01860"
     CDS             complement(12089..13483)
                     /gene="miaB"
                     /locus_tag="PLD98_01860"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQA05028.1"
                     /translation="MSTFSGNEYSRLIKTLLDTNYPVYSPKAFVHTFGCQGNLADSER
                     MKGMLSEIGYEFTDDVNEANLILFNTCSVREHAEDRVFGNVGNLKKLKAEKPDLIIVL
                     CGCMMQQKHVAEKIKKSYPYVDIVFGTYHMQRLPEFIYKVLATRERVFEITDNPEAEI
                     VEGLPVYRDISYKGWLPIMYGCNNFCSYCVVPLVRGRERSRDPEDVINEAKELIKSGA
                     KDITLLGQNVNSYGKNSPFNMDFPELLRRINALEGEFIIRFMTSHPKDCTPELLKVMA
                     ESEKVAKHLHLPFQSGSNRILKAMNRKYTREHYLELTTLARELMPELAITSDVIVGFP
                     GEQYEDFTQTLDLVERVKFSSLFTFIYSKRKGTAAEKLPDPVSREEKGRWFNELLKVQ
                     ERISAEIRQSMVGKTYKVLAENKTKDLDGCITGRTEGNMNIDFLGDESLIGNFVKVKV
                     NKVENLNIKGEMIP"
     gene            complement(13631..15388)
                     /locus_tag="PLD98_01865"
     CDS             complement(13631..15388)
                     /locus_tag="PLD98_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQA05029.1"
                     /translation="MENAFYPVREIRDFKDLLNQSAELFGARPAFSLKKNGEYVDVTY
                     NEFKNDVFAFGAALNSLFGLEGSRVSLIGENSYEWCVSYLAVTCGGGVIVPIDKDLSF
                     EDIASIISVSKSKAIIADEAKTQLLIENKDKLSEDLIIITKEKEKNEGSTKQVLDPAK
                     IQYSFKSLKEKGKASPQDLEAYERIVPDPNAMSVLLFTSGTTGTSKGVMLSQYNICSD
                     IMALAGVVKINPTDKLLTVLPLHHTYQCSLGFLMMMYSGGNVAFSEGLRYIVQNMQEF
                     KPTLFLTVPLMLEKIHKRIIKAASSKHGGKLALTFGKAISSFGAAFGINIREKIFGEI
                     QKNFGGELRMIITGAAPISPTVVKDFQAFGIPVYIGYGLTECSPLVIGNNDRLQLPDS
                     VGVPLPGVEVKILNPDSDGVGEIAIKGPMVMLGYYENEKATAEVFEDGWFKTGDLGTV
                     DENGHYRIAGRSKNVIVTKTGKNIYPEEVEYHLNTNPLIAESMVVADDGDEDGDTTVR
                     AKIFPDIEAIKEKLKLQSVTKEQITKVIQDVIKEVNKKLPKYKNIKSFDIRESEFVKT
                     TTHKIKRFANIGKEEEENE"
     gene            complement(15435..16142)
                     /locus_tag="PLD98_01870"
     CDS             complement(15435..16142)
                     /locus_tag="PLD98_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05030.1"
                     /translation="MLNTKKRLVAILVSVALIMSLAAITFAVLGNRPGSSDGKFESNY
                     VGRVNITAEKTSFNIDKLQDGQKENISLTLSVKKTEPEFYAKIVSTSIKGLNHDYAVW
                     TSVEGDKDFPEDAILPVGDDGEPKALKWSLSAPFTVPDIFDTKPYIEIRLLSGVSELL
                     ATESIVKIPLKITIINKKELSRLVNIGHSVINQTGANPDSELYQAVDEAAKLLLTQCT
                     QQEVDSAVNNLKNHLGY"
     gene            complement(16232..16840)
                     /locus_tag="PLD98_01875"
     CDS             complement(16232..16840)
                     /locus_tag="PLD98_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4093 domain-containing protein"
                     /protein_id="HQA05031.1"
                     /translation="MLKTDRAIIVEGKYDKIKLSGIVDALIIETDGFRVFKDKELQKL
                     IKRIAEEKGIIIFTDSDAAGFKIRAFVASIVPKGTFLHAYIPDVFGKEKRKTAPSAEG
                     KLGLEGIPDEIIVNALKRTGIENEGNAQLSPHKEITAFDLMSDGLCGKDGSKKLREEF
                     LKFLGLPAHLSTSSLIKLINSIYGYDLYRQMLSDFLCREKAK"
     gene            complement(16849..17883)
                     /gene="holA"
                     /locus_tag="PLD98_01880"
     CDS             complement(16849..17883)
                     /gene="holA"
                     /locus_tag="PLD98_01880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQA05032.1"
                     /translation="MPLFDETRLKNYLKEPSNVNVFLIYGEDDYLKDYYSAFIRDRAV
                     DPAFSAFNLHGFEADEMDISSVWDCATAYPLMSERSCVFIKGMKLAALKKDAVEDIKR
                     LISEIPDFCTLIVKAEEGAEEEKRNAAVKDMVGAFSKAGIAACLSKRQTNSIVTLIMN
                     GAKSRGSSITKEAAAYLVEIVGDDLLVLMNELTKLAAFAGKDGITKEIIDKVAVKSVE
                     ASIFDLSAAIIEKNSDKAYRILSALKLDKVSPQMIIGTLSSYFADLYRAKVADKERAG
                     LSNIISAYDYKNTAFRLEKAMKAAGKIGMKSIRKALENLTEADSKIKSTGADSFIVIE
                     SLVASLLTIN"
     gene            complement(17885..20059)
                     /locus_tag="PLD98_01885"
     CDS             complement(17885..20059)
                     /locus_tag="PLD98_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HQA05033.1"
                     /translation="MPFKQSVMLLFVSLLFSLAFTLLCFKNERLRTLAAVFLCFSSAI
                     LLTVSVAELKYNKALESVGSNKEIVGRVVNYEQSGGRNYYIIKTELIDNEEKRVNLRL
                     YSRTPIYAEVNDMVKFKADSVYKLGGSDTAKRSYKADSLFLGAYTYGEITVKPAKTKT
                     LFTSISKAREYIKDIIKTNFPEETEGLMTGIMLGDTSGIDSGLLNEVRKTGAAHILSV
                     SGLHVSLWSMFAYQLLTRLKRRTLSGIVSIAFVFFVVAVTGFSSAAIRAGIMLSLFYL
                     GEIFNKNSEPINSLGFAAFVICFINPYTAADLSFLLSFFATLGILLAAGKTNEFAGKI
                     KIKLKSLKRLVSGIVTAFCISLAAVLFTMPVSVLAFGGISFIAPLSNIISILPTEAAM
                     LFCGLAIISSPIKFLSSALYYIASFLLDCFILINSLFAKLPFAYINISKGVLAAAASA
                     FPICLLFLKLLDREKNLSLLDKKNKNMRNILRAFLASIMVLLLGVNISYFSDFNKLKI
                     YVPDAGNSTAVILTLNGRAAIIGCGGEYNVPYEINGRLYRENIYSLDLLVLPRDKETE
                     IGFAPKVIKEHTPKTLIASKSVDPVLFMHNKNIDSQQSDTAKIVLWGSVVLEYINNDS
                     LSCARISFAGKAVLICFTPSSDFTGFESFSDADILIARSAYPESLRNKVFERVIISAD
                     SYKIGSEKKFEKNSKNPVLLTASDSGVEISIKNKAFSIKTGD"
     gene            complement(20201..20734)
                     /locus_tag="PLD98_01890"
     CDS             complement(20201..20734)
                     /locus_tag="PLD98_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQA05034.1"
                     /translation="MEKYKMTIAGLERELPICRINDNLSIAAFIMFNDVELTIAAAKE
                     LLAKCPEFDIILTPEAKSIPLAYEMARQSGKPYLVARKGKKLYMHDPVRVEVKSITTE
                     NNQQLWIDQSDVELMRGKKVLIVDDVISTGNSLNAVIELAKRFGAEIAACAAVLAEGE
                     AAERQDIIFLEPLPLFD"
     gene            complement(20798..21139)
                     /locus_tag="PLD98_01895"
     CDS             complement(20798..21139)
                     /locus_tag="PLD98_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HQA05035.1"
                     /translation="MDCIFCKIIAGEIPSKKVYEDEHVLAFEDISPRAPVHILLIPKE
                     HIVSAAEINGENSAYVAKIFEVIPKIAEQMGVKESGFRVVTNCGASAGQSVMHLHFHL
                     LGGRSFSWPPG"
     gene            complement(21158..>22183)
                     /locus_tag="PLD98_01900"
     CDS             complement(21158..>22183)
                     /locus_tag="PLD98_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HQA05036.1"
                     /translation="SVFTGAKVSASVCKKSREEVKKNHTSAHLLHAALKKVLGQHVEQ
                     AGQSVTPELMRFDFTHFSALTSEEINKVEEMVNQAIMSAQDVKTELMGLDEAKAKGAV
                     ALFGEKYSDVVRVVSAGDFSSELCGGTHVSNTGEIGMFKIISETSVAAGVRRIEALTG
                     LNLLEYIRKNTAVLYETAEILKCGDVYEITEKAAQLNKELKQKDKEIAALKAEIAAAK
                     LSSELKVRAEIDGIKLITANIGEAAADEARAAADRIKESGDDVVAVIAATQSEKGTCT
                     FCAVCGSAALKAGAHAGNLVKAVAAVAGGRGGGKPESAMAGGKDISKVDEALDSAEEA
                     LKAMLNK"
     assembly_gap    22184..22193
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22194..>22839)
                     /locus_tag="PLD98_01905"
     CDS             complement(<22194..>22839)
                     /locus_tag="PLD98_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HQA05037.1"
                     /translation="EVAESVIKFSSGAYPDLAEKRDFIINIITHEEENFLKTIDAGMK
                     LLFEMLEGKDSLSGDDAFKLSDTFGFPIDLTEEIVNEQGKTLDKARFEELMQLHRQKA
                     REARKFSGSDAWKDSAAALKDYPETEFVGYDTDSADTKIIALLSNGEIVETIEKDSEE
                     TAIVLEKTPFYALGGGQCGDIGKITGDGFEFTVTDTIKTAKNVYIHKGYISEGVS"
BASE COUNT         5596 a         5387 c         4798 g         7048 t
ORIGIN      
        1 gcggcggggt gaacaaccat gccctttggc ttattgacca caagcaggtg ctcgtcctcg
       61 taaataatgt caaggggaat gtcctgggga gaaagctcaa gttcctgcgg ttcgggcaca
      121 tctaagctta tgcagtcacc gcttttaact ttaacgcact ttgaaataag cttattattg
      181 acgctgactt tgccatcgtc aatcagcttt tgaatatatg accttgacag ctcgggctga
      241 gtcaaagcca aaaatttgtc tattctttct ccctcatatt cagagctgac aatgagttct
      301 attttgttca tgccgtctca tccgatgttt tctcggaaac agattcgctt tcttcgcttt
      361 catcagcctt ttcgctgatt tctccagttt ctgcgggaag ggcgttttcc tcagaaactg
      421 cgctttcggc gtgaggcaat tcttttttgg agtttttaaa ctctttgaaa atgtcataaa
      481 gcaaaaagcc aatcagaagg aatgaaccta cagttacaag gcagtcggca aaattgaata
      541 cgggaaaatc tatgaatgcg ggcagagcct gaatataatc cacaacatga ccgttgaggg
      601 ctctgtctac aagatttccg agaccgcctg agattattaa gataaggctg acaatctcaa
      661 tcttgctttt aatctttgcc gaaactaagt aaataatcag tcctaaaaga ataaccccgg
      721 taaagtaaat aagaaattgg gaggaacctc tgagtatgcc aaaggcggcg ccgggattgt
      781 gaacataggt aaaatgcaga ataccgtcaa tgaacggtat ggtgtctatg gggcgcagat
      841 atgttttggc aaagtatttg ataattaaat caatcgcaac aagaacaatt ataataggta
      901 aataagtaag taacgctttc agtctgcttt tatttttttg caaaatcagc cctccaaagt
      961 ttttaggcta ctcaaaaatg ctgaaatcaa tttcaaagcc ttttatattt tctgtttttt
     1021 cctcgacatc ttcttctgct gtatcgggct cggcaaacag ctctgagggc tttgcgtcgg
     1081 aaagatcatc ccagctgtca tcctctgtgt caaggtcaag gtcgtcgtca tcaaaaatgc
     1141 ttgtatcaag ctcaaagccc agatcgtaat cctcgtcttc gacagcttcc tcagcatcaa
     1201 caattgcctc gttttcaagg tcgagcgatg caacagctga cttcttcatg tctctgattt
     1261 tattgaaaat ctgaacataa gactcggata aatcctcagt attgtcaaag tagctctctt
     1321 ctgtcggctg ctctgattct tccgtttctt cctgagcttc tgtaattatt gtttcaactt
     1381 caatttctgc ctgcttagac tctgcggaat cgacggacaa gtcatcccag tcgtcatcaa
     1441 agctgtcgtc gatttcaaag ccttcttttt cctcagaagt ttctacaatc gtaacttcgc
     1501 tttgaggttc ttcaatctca gactcgacga tttccgtttc ttcaactatc gcgctagtat
     1561 caagatcaaa gcctgaaata tcctgccagt cgtcctcttc gtcaacggac tcgatagttt
     1621 cggcgacagc ctcttcatcg ttcacaagga cttcggcgtc taaagattca gaaacagctt
     1681 cttcgtcaat ttcaaaggct tcgggttctg tttcttctat ggcttcgact tcctcagaag
     1741 tatccacaga aatctcttct gctgcgccaa ggtcgtccca gtcgtcatca gactcatgag
     1801 cctctgctga ctcctctact tctgcggctt cttcggcagc ctcagtttcg ggagcttctt
     1861 tagcctctgc ggtctctgac tcagcgtctt cttctatatc ggatatttta acgccttctt
     1921 ctgattcttc gtttaaggcg gttatttcgt cgcttacggc tttggaatca aatgccttga
     1981 atccaaagta cataagaata tcgtcggctg ttttatcagt gaaatcaacc gaatcaatat
     2041 cctctataac ggggacctcg taatgctctc cttcagcgtt aagaagtcct tcgtattcgg
     2101 gagcgatatt ctttaaatgc tcatccatgc tcagcttcat ggcttcgctg agaaccttgc
     2161 agttgccttt aatcttattg taggtctctt ccatgtgctg gagcttgggg aggatatcct
     2221 caagcctcat tttggctgcc attgttattg agtcagactc atttttggca gaaacaagca
     2281 gctccgccgc catatccttt gtggatgcca ttaaatcctc ggcggcagtc tcagtggtgt
     2341 gctttaattc ctcggctttt gcctctgcct cagccacaat gctttctgcc ttggcagcgg
     2401 cctcattaag aatgctctcg gccttagcat ttgcttcaga gataacactc tctgcctgtg
     2461 cgttagcttc agacacaaca ctttcagctt gtgcgtttgc ttcggaaaga attctttcgg
     2521 cctcggcatt tgcgttattg attatctcct cagcctttgc cctagcctcg gcttccatat
     2581 tctcagactt ttcaagaact gatgccaaaa gtgcgtcgga cttgtttctg gcgctctcta
     2641 tgtatgtctg ggcgttgtcc tttaacgcgg cgatttcctt ttcggcgttt tctctttcta
     2701 tccttgaaag ctcctcagcc ttggcctttg cggtttcaag aaggttctcg gcggacttct
     2761 ttgcttcctc aattatacgc tcggcttctt ctacggcttt agccctaatt tcctgagcct
     2821 ctgtgctgtt ttgaaggatg agctcctctt tctcttttat tgaagcaagg gatgagcttt
     2881 caatctcctt agccttatcg gcagcggcgt taagaagctg ccttgcggtt tcctctgcct
     2941 ggctgagctt ctcgttcgcg ctttcttcgg cggactttat aatctcctta gccctgtttt
     3001 gagcgtcaat cttaattaaa gacgcctcgc gccttgcctg gtcaagtgtt gtggaaatgc
     3061 ttgcccttgc gtctgccatg agcttttcgg cagagcgctg agcagcggca agagatttct
     3121 ttataatatc ctcttcacgg cgatattcct caatcctggt tgcaaggatg cttattttgc
     3181 gaaggagctc gccgttctct tggaacattt gggcataaga gcgagaaacc tcctcaagga
     3241 aggcatcgac ttcactcttg ctgtaggctt gaccgtctat caaattaaag ctctgagact
     3301 ttatcttatc aggagtgagc atttatatta ccgtccattt ctccctaata cggaaagtat
     3361 agtttatgag cttatatgaa gaagactccg ttgctttcaa gctcgtcaag taaatcaccc
     3421 attacatcca cattgtaagg ggtaattata aatgtgctgt tggcgattct cttgagttga
     3481 cctccgtttg cgtatgcaac gccgctgagg aagtcgacaa gacgccttgc aacatcgcgg
     3541 cttgcggact cgagattgag aactaccgtt ctcttttcat taagattgtc ggcaatggct
     3601 cgtccatcct caaagcgttc gggctttgtg agcacaacct gcagctttgc ggttgtgtga
     3661 atgtccacaa tcttattgga gcctgacttt accgatgtcg cgggcttgga ctcagcgcgc
     3721 agaatgctct ttctgggctg gggttcttct tcctcttccg gggaatatac taaaaggtca
     3781 ttagactcaa catcgtcata atattcgtct tcgtcaatat tgacaatctt tttaagctta
     3841 tccactaatc ccatacatta tcctccccta atatattctt gctccgaaca gggcggagcc
     3901 gattcgtact aagttggagc cgcattttat tgcagcttcg tagtcttgag acatacctgc
     3961 ggacaatatt tgcatgtcta cattatctat ttttttatct ttaatgtcaa taaacagttt
     4021 gctaattgca tcaaaatacg ccattgcttc cttttcgctg tcgcaaacgg gaggaatgca
     4081 cataagaccc tttattttaa tgccctctag ctctgcaatt tcgtaaattc cgtcaatcac
     4141 ttcatcgggg tcaaagcctg ttttgctttc ttccctgcct atgtttactt caacaagcac
     4201 aggcattgtg acgcccgctt tgactgcccg ctttgagatt tccttagcca gtttcaccga
     4261 gtcaaggctt tcaatcatat ctaccttatc aattatcttt gataccttat ttgtctgcaa
     4321 atgtcctatg atatgaacct ctggcttttt ttcgtttttc attatttctt cttccttgct
     4381 caggtactcc tgaacacggt tttcgcctat caaatcaatg ccgaggttta ttgcgtgttt
     4441 tatatatatg ggctctacgg ttttggttac ggccataagc cttatttcat cgggatttct
     4501 gtttaccgaa agagctgcct tttctatatt ggcttttatg accttatagt tctcttctat
     4561 tattctcaat ctctcgtcaa gaaatgacct ttcctgcata aagattttta ccctccacaa
     4621 ttatttcatc gtataatctc agatgccctt cgggattttc gggcgtggta gctattgaaa
     4681 tatcgtcatc ggaataggta atgttgattt ttctgaaggt tacgaggttt cctctgagaa
     4741 tataaactcc cttttcaccg tcaacgattc ttatagctga attgggaaca gcgtagccct
     4801 catgcttttc gagccttatc tcagcccttt cgattctgag ctgtgaaagc tcctcgctca
     4861 tctgccttga agtaaagata acgggcgttt ttccgtcttt tgtttcgctg attttcgcga
     4921 ctttaacctt tatttcaccc acggatgagc tggggaaatt aatcgatacg gtggagccga
     4981 ccttaaggtc agccgcaagc tccgccttta aaaccgcagc tatgtaaaaa tcgtagccgc
     5041 taatgagttt gcctcttaca tttgacggaa cctgctcagg ctcttttttt atcagctctt
     5101 cgatttcttc gcagctgagc tccagcgctt tttcaaaggg aaccgcgttt tcatagccgt
     5161 cggcggtgga aacatagtat ccgggaccgg tagcagaaac aaatttcgtg tcatggcggg
     5221 aggaggataa ggcgctgagc cttgatttaa gttcactgag cttctgagag taatccacct
     5281 gttcacccgt tgccctttgc cttgcggtga tggtttctct taccctgtct atttgcgcct
     5341 ttaagtcgct gagctttccg ctttccgcgg cagttatgta atttagaacg ctttgcttaa
     5401 tattggagtt tagctgtgtt aggtcgcttt gaaaattaat atccgacttt gacagggctt
     5461 cataataagc tatctcattc ttaatttgtc tttcttctat aaagcttttg gcgtcggcat
     5521 cgtttgagaa aatcgcggca acattgtcgc cgcccgtaac ccttgtgccg tctttgacaa
     5581 gggggacgac gctgccgttc gcgtcatata ctatgtattt ttcatccctg ataacaaaga
     5641 tgtctactac agcggattta taaaccgtct ttgtaagcgc ggttgtggtt ttgacgggaa
     5701 ccgaatatat tttatagatt tgagcggcgg cgtaaattat taaaatcagt gccgcggcgc
     5761 cgattcccgc gagcttgaaa acggagctgt ttataaagtt tatctttact actttgcttt
     5821 tcggctttct gcgcgctcta ttcaagattt tccctccgtt ctgcctgaga ttatcgataa
     5881 ccgatcaaga attttgactg cttttgcaac tgacaagaaa atcctttgac atttttattg
     5941 cgtctgctgc cacgctgtca aagctgtcgt ggcagtctac ataaaaccag ttgatttctt
     6001 tattcttcct gaaccaagtt agctggcgct tagcgtatct gcgggtttct cttttaagat
     6061 tatcaacagc ttcttccaaa gtcatttcgc cgttaatata gggcaaaagc tctttgtgac
     6121 ctattgccct gcaagcggtt gtagcgtccg ataattcctt gtatctggcc gcttcttcca
     6181 aaagaccttt ttcaagcatt atatcaacac gcctgtttat tctgtcataa agcagggctc
     6241 tgtttttgaa atcaagaccg agatataaga cattataggg cgagccttca tcggtttctt
     6301 gcttttcgct catgggtctg ccagttaggt agtaaacctc cagggccctt ataattctgc
     6361 ctcggttatt tatatggagc ttgtccgccg tttctttgtc cacacgcttt agttcttcaa
     6421 aaagggcttc aatgccttct tctttttcac gctcttcaag ggctttcctt actgtcatat
     6481 catgctcttt aagcgtatag atatggggtt taagaagggt atcaacatat agccccgtgc
     6541 cgcctgcgac tatcggcacc tttcctcttt tgtttatatc ggcgatgtat tccgatgcct
     6601 tttcggcgta gagagcggcg ctgtagcgga cggatgtgtc cacaaggtcc atcatgtagt
     6661 ggggtatgcc ctgacgctcc tcggcggtag gctttgcggt ggcaatatcc attcccttgt
     6721 aaacctgcat agaatccgcg gagacaatct caccgtcaag agcctttgca agctcaattg
     6781 caagcgcggt tttgcctgat gccgtgggtc ccacaactgc aattacagga attaagttca
     6841 ccttttcacc gccctactga attctgccaa aaagcttttc aagctcattt tttgtaagct
     6901 caatcattac aggtctgccg tgggggcagt acctaatgtt attgtcattt aataccctat
     6961 caacgatttc agccatttcg tatttggaga tactcttgcc cgacttgatt gccgacttgc
     7021 aggctgcgga atgataaatc ttatcaatct tttccgaact tatcatattg gggctcgagg
     7081 caagattatc ggcaatttcc tcaaaaagct ccctaatatc cgctgacaaa agatcaacgg
     7141 gacatgagcg aacaagcaca ctgccatcgc cgaaatcgga cacatcaaag ccggctttct
     7201 ttaaaagctc aagattatct attacagcat tgtactctct gggacccaaa tcaatcttga
     7261 cggggctcat gagaagctgg gatgaatagc cgaaaaggtt ttcttttagc ttattatata
     7321 tcattctttc atgggctgcg tgcttatcta tcagcagcag cttgcctttc atctcagcga
     7381 taatataggt attaaacgcc tcgccaatga ttacgggagt atctttaatt tctgcctttt
     7441 tttcgggtgc tgggccattt tcgtttttgg tttccacctg ctttgaggca gactcaaact
     7501 tttgagttaa atcaattaaa ggctcatctg taatatcttc ggatatttca aatctaaggt
     7561 tttctttatt ggaatcgatt ttcgcttttg tattgctctt taataaaaat tcgtcggggt
     7621 tattatagcc tttataatca agagctatgg ttggagttct aagtctcaga gttttatcaa
     7681 gactgtcctc agggtttttt tcctcaattc gcttttctat ctcagccatc tttgctaaag
     7741 tttcggtttt ctctttagcg gcgcggctga acaagtcctc tccttcttcc gccttgcttt
     7801 catttataaa gtttgcctta taagcgtttt tattctttgc cgcaatatcg cccgacaaag
     7861 ctacggaaga cggagcgtca aactttgcaa gtgagctttg cgcggcgtgg aaaataaggg
     7921 agaatatccg cttttcatcg gaaaagcgaa cctcggtttt tgcgggatgg acatttacat
     7981 caactgtttc gggagggatt gaaatattaa ttacgcaggc ggggaatttg cctaccataa
     8041 tggaattctt atacgccgta tcaagggcgg aggctccaac ggggattttt acaaacctgc
     8101 cgtttatgta aaaatactgc tgattcctgt ttaccctcga aaacgaaggc agagagatat
     8161 agccctcagc ggaaagcccc tcaaagctgt aggacaaagg aattaaggta tcggcgaatt
     8221 ccttgccgag cgcggaatga attgcggaaa tcagctttcc gtcaccgggg gtaaagagaa
     8281 cctgcttacc gtctttaatg agcctgaaac ttatatgcgg gtgggaaaga gcgattttaa
     8341 ggcaaatatc ttgcacatag cccgcctcgg tggcgtcttt ttttaggaac ttcatccttg
     8401 cgggcgtgtt ataaaataaa tcccttacaa ttattgtagt gccaacgggg cagccggcgt
     8461 cgtcaattga aacttcctgc ccgccctcaa taacataggc ggtgccgact ggagaatcgg
     8521 cagttctcgt caacatattc acccttgaaa cagcggcgat tgatgccaat gcctctcccc
     8581 tgaatcccaa tgtaaatatg gaattaaggt ccgtactctc cttgattttg ctggttgcgt
     8641 gacttaaaaa ggcggttctt acatcctccc tatcgattcc gcagccgttg tcggtaaccc
     8701 ttatgtattt aattccgccg ctgtttatct cgacggtaat cttatcggct cccgagtcaa
     8761 tggcgttttc gagcagctct ttcaccacgg aagcgggctt ttccacaact tcgcccgcgg
     8821 caataagctc cgccacgctt ttggggagaa catttatttt aggcattctc tcaccgcctt
     8881 tctgggaagt ttaaatcagt taagcctttt aatgataccg tgaagtattg tcatagcctc
     8941 aattggcgta agaacattta tgtcaagatt tttaatttca tcaataatag caaggtttgc
     9001 gttgtctgca aaagtattgc cgaggctcag ctggttgctg tcagactgaa cggatttcgg
     9061 cacatagtga taatgaccct tgtttgactc aatgtcgtca aggatttctt tggctctgtc
     9121 ggcaaccttc ttgggaacgc ccgcaagacg cgcaacctca atgccgtagc tgccgtcgga
     9181 ggctcccctg actatgcgtc tgaggaaaat aatatcatcc ccgcgctttt ttgcggtgat
     9241 atagtagttc tttacgccgc ttatctcgtc ttcaagctgc gccagctcgt gataatgggt
     9301 ggcgaaaaga gtccttgcgc cgatgttctt tttgtcggtt atgtactcaa gcaaagccct
     9361 cgctatgctc attccgtcat aggtggaagt tcccctgccc acctcgtcga gaattattag
     9421 gctctttctt gtagcgtttt ttattatgaa tgcgacctcg gacatctccg acataaaggt
     9481 ggactgaccc attgaaaggt cgtcggatgc gccgattctt gtgaaaatgc cgtccacaat
     9541 gccaataaca gcagattcag cgggaacaaa gctgcctatc tgcgccataa ttgtgatgag
     9601 cgcaatttga cgcatatatg tggacttacc cgccatattg ggacctgtaa tgattacggt
     9661 tctgttgtct tcgcaatcaa ggagagcgtc gttcggcaca aagggctcgt ctgtaaattt
     9721 ctcaactacc gggtgcctgc cgtttttaat ttcaagcctg tcggaaaggt cgactatcgg
     9781 acgggtataa ccgttttgag atgccgcata ggcaagggag caaagtgaat ccagcttagc
     9841 aatagccgat gccgtacgcc tgattctgta ctgctggtcg gcggtctttg ctcttatagc
     9901 ggcaaaaatc tcatactcaa gctttgcgat tctctcctga gagcccaaga ccttgctttc
     9961 aagctcttta agctcggggg ttatgtatct ctcgcagttt gagagagtct gctttcttat
    10021 gtagtgctct gggacaagct ctttatatga atttaaaacc tctatgtaat atccgaaaac
    10081 tctgttgtag cctatcttca gtttcttaat gcccgtcttt tgctgctcct cttcctccag
    10141 cttaacaagg aagctcttgc cgccggatac aatggagcgc aactcgtcca attctgcgtt
    10201 aaagccgtcc tttataagcc cgccttccct taccgtaaag ggcgggtcgt ctataatcgc
    10261 ggtttcaata agctcataaa catcgcttaa aaggtcaatg tccctttcaa tttcatttag
    10321 catcttgctg ctaaatcctt taagcgcggc tttaagctcg ggcaaagctt ttatagtgtt
    10381 tgaaagaacc ttgagctctc tggcgtttgc ggtttcgtaa actattcggg tgatgattct
    10441 ttcaatatcg ctgatgccag acagcaaatc tttaatggac ataagcctta ttgtgtctga
    10501 ataaagctcg tccacggcgt ttaggcgcat tgagatttca ggcaaggtca ccaatggatt
    10561 ttctacccac tggcgaagca ttcttttgcc catggcggtt tttgtggagt caagaaccca
    10621 aagaagggag ccttttacct ctctccttct ttcgctttga gtcagctcca aagtgcgcct
    10681 tgcgttctcg tcaagcgcca tatactttga gtcgttgtaa acctttattt cctcaatgtt
    10741 tgaaacattg ttcttctgcg tttcgtacaa atatgccatc agcgcgccga cggctgagat
    10801 aagcattttc ttttcaaaga tttcatcgtc cagcttggta aaatgctctt tgatgatttt
    10861 tgacgatgct tcaaaatcaa acctttcatc atcaaaggtg tcaacggcaa gggtaatctt
    10921 attcttcaaa aaatcaaaga tggtttttct ctttgaggac tctctgttag ctataagctc
    10981 ggcgggggta aaccttgcga tttcgttaat tacaaggtta tctacgaatt tgccttgaag
    11041 ctccgccgcc tgcagatcgc ctgtggagat atctgcaaaa gcaacgccag ccgcattatt
    11101 ctctacaaaa atgcagccta tgtagttgtt tttgccctcg tcaagcatgc tgctttccgt
    11161 aacggtaccg ggggttataa cccttacaac ctctcggcga acaataccct ttgctgtggc
    11221 ggggtcctcc acctgctcgc aaatagcaac cttgtagccc tttgcaacaa gtcgggctat
    11281 atatgattca gcgctgtgga agggaactcc gcacatggga gctctttcgt cttgtccgca
    11341 gtcgcggcct gtcaatacaa gttcaagctc tctggacgcg gtaacagcgt cgtcaaagaa
    11401 catttcgtaa aaatcgccaa gtctaaaaaa cagcaaggca tctttatact gttcttttat
    11461 cttgagatac tgagtcatca tcggagaaaa gtctgccatc aggacacccc actgagttta
    11521 gtaaaacaaa acatccatat cagttttaag gcaatgcaaa gtttttagct ttacattgcc
    11581 ttattttcaa gtacgccaat tagcagccct ggcaggagga gcaggagcca tggcagcccg
    11641 caccctcatt catctggggc tcgcaggttt ccgggtcctc gccgtttacg cacatagcaa
    11701 gaatctgcat gatatagttc atgagttcgt caaggtcgcg ccttgtattc tcataattaa
    11761 tcatgttggg attctgcatg atttcgctgt aaaggcttct gaactcgtcg tcatactgag
    11821 ccagtttgtt ctcgtcgggc tcaggctgct ctgcctcatg ctgataagaa agctgaacca
    11881 gattcagctt gcctataagg tcattgagcc cttcgtcttc ctcgtttgcc tttcttgcag
    11941 cctggaacgc aaggtatctt tcatcctgct gaatggctgc gccaagttgt cttgtaagtt
    12001 tgattaaatc catttcttta ctccttttgt tggtttatat aacgcataaa gcgaaatatt
    12061 cagttgtcaa gtgtttctct ttacaccgtt aaggaatcat ctcgccttta atattaagat
    12121 tttctacctt gtttacctta acctttacaa agtttcctat caggctctca tcacccagga
    12181 aatcgatgtt catattgccc tcagttctgc ctgtgatgca gccgtcaagg tcctttgttt
    12241 tgttttcggc gagcacttta taagtttttc cgaccatgga ctgtcttatt tccgcggaaa
    12301 ttctttcctg tactttaaga agctcgttaa accatctgcc cttttcctct cggctgacgg
    12361 ggtcgggcag cttttcggcg gcggttccct tgcgctttga atatatgaag gtaaacaagg
    12421 aggagaactt tacacgctca acaagatcta aggtctgtgt aaagtcttca tactgttcac
    12481 ccgggaagcc gacgattaca tcgcttgtaa ttgcaagctc aggcataagc tcccttgcaa
    12541 gtgtggtcag ctcaagatag tgctcccttg tgtattttct gttcatcgct ttaagaatgc
    12601 ggtttgaacc ggactggaag ggcaaatgca gatgctttgc taccttttcg ctctcagcca
    12661 ttacctttaa cagctcggga gtgcagtctt tagggtgaga ggtcataaat cttatgataa
    12721 actcaccttc aagggcgtta attctgcgaa gcagctcggg gaaatccata ttaaagggag
    12781 aattcttacc gtaggaattt acattctgac caaggagcgt aatatcctta gctcccgact
    12841 ttataagctc ctttgcctca tttataacat cctccgggtc gcggctcctc tccctgcctc
    12901 tgacaagcgg caccacgcag tagctgcaga aattgttgca gccgtacata atgggaagcc
    12961 agcccttgta gctgatatcc ctgtaaacgg gaagaccctc tacaatctcc gcctcgggat
    13021 tgtctgtgat ttcaaatacc ctttcccttg ttgcaaggac cttgtaaata aactcaggca
    13081 gtctttgcat gtggtaggtt ccgaaaacaa tatctacata agggtagctt tttttgattt
    13141 tttccgccac atgcttctgc tgcatcatac agccgcaaag gacaatgatc aaatcgggct
    13201 tttcagcctt cagctttttt aaattgccaa cattaccgaa aactctgtcc tctgcgtgct
    13261 ccctgacaga gcaggtgttg aaaagtatta aatttgcttc attaacatca tcagtaaatt
    13321 catagcctat ctccgaaagc atacccttca tgcgctcgct gtcggcaaga ttgccctgac
    13381 agccaaaggt gtggacaaat gccttgggag aatatacggg ataatttgta tctaaaagcg
    13441 tctttataag acgggaatat tcgttaccgc taaatgtgct caatttatcc tccgattcaa
    13501 ctaaacttaa gcatatattg tgtttaatat actatcttag tttaatataa atttgaaaaa
    13561 aagtaaactg attaaatcca tttgccttaa attaagttta tcggctgccc caaaaacggc
    13621 agccgattta ttattcgttt tcttcttcct cttttccgat attcgcaaag cgcttaatct
    13681 tatgggtcgt ggtctttaca aactcggatt ccctgatgtc aaagctcttg atgtttttat
    13741 acttgggaag ttttttgttt acttccttaa taacatcctg aataactttg gtaatctgct
    13801 ctttagttac actctgaagc ttgagcttct ccttaattgc ttcaatatcg gggaaaatct
    13861 tcgctctgac agttgtatct ccgtcttcgt ccccgtcatc ggctaccacc atagactcgg
    13921 caattaaggg atttgtgttg aggtggtact caacctcctc gggatatata ttcttgcccg
    13981 tttttgtgac aataacattc ttgctgcggc ccgcaattcg gtaatgaccg ttttcgtcaa
    14041 cggtgccgag gtcgcctgtc ttgaaccagc cgtcttcaaa tacctctgcg gtcgcttttt
    14101 cattttcata atagcccagc attaccatcg ggcctttaat tgcgatttcg ccaactccgt
    14161 cgctgtcggg gttaagtatt ttaacctcaa cgccgggcag aggcacgccg acggaatcgg
    14221 gcagctgcag acggtcattg ttgcctatta caaggggcga acactcggta agaccgtagc
    14281 ctatatatac gggaatgccg aaagcctgaa aatccttaac aaccgtagga cttataggcg
    14341 cggctcccgt aataatcatt ctcagctcgc cgccgaagtt tttttgtatc tcgccgaaaa
    14401 tcttttctct gatatttatg ccgaaagcag cgccaaagct ggaaatcgcc tttccgaagg
    14461 taagagccag cttgccgccg tgcttgcttg aggcggcttt gattatccgc ttatgtattt
    14521 tttcgagcat aagcggaact gtcaggaaca gcgtgggctt gaattcctgc atgttttgta
    14581 cgatatatct gagtccctcg gagaaagcca catttccgcc cgaatacatc atcatcaaga
    14641 atcccagtga gcactggtag gtatgatgca gcgggagaac cgtcagcagc ttgtccgtgg
    14701 ggttaatctt tacaacaccc gcaagcgcca tgatgtccga gcaaatgttg tactggctca
    14761 gcattacgcc ctttgatgtg cccgtagtgc ccgatgtaaa aagcagtaca ctcatcgcat
    14821 tcgggtcggg tacaattctc tcgtaagcct ccaaatcctg cggtgatgct ttgccctttt
    14881 ctttaagact cttaaaggaa tactgtatct tggcgggatc gagaacctgc tttgtggagc
    14941 cttcgttttt ttctttttcc tttgtgataa tgattaaatc ttcgctcagt ttgtccttat
    15001 tctcaataag aagctgagtt tttgcctcgt ccgcaataat tgcctttgat tttgaaacgg
    15061 agattattga agcaatatcc tcaaaggaca aatccttgtc gatgggaaca attactcccc
    15121 cgccgcaggt aacggcaagg tatgaaacgc accattcgta tgagttttcg ccgattaatg
    15181 atactctgct gccctcaagt ccgaaaagac tgttaagggc ggcgccgaaa gcaaaaacat
    15241 cgtttttaaa ctcgttgtaa gtaacatcca catattcacc gtttttcttt aaagaaaaag
    15301 cgggtcttgc accgaaaagt tcggcgcttt gattcagcaa atccttaaaa tctcgaattt
    15361 ctctgaccgg ataaaaagcg ttttccattt actatccctg cctttttatt tttttggagg
    15421 gaacaaacct aaatttaata accgagatga ttttttaaat tatttacggc agagtcaact
    15481 tcctgctgag tgcattgagt taaaagaagc ttagcagctt cgtcaactgc ttgatagagc
    15541 tctgagtccg ggtttgctcc ggtttgattt attactgagt gtcctatatt tacaagcctt
    15601 gaaagctctt ttttatttat gattgtgatt ttaagcggga tttttacaat gctctcggtt
    15661 gcaagcagct cgctgacacc gcttaaaagc ctaatttcaa tgtatggctt ggtatcgaaa
    15721 atatctggaa ctgtgaaggg agcggaaagg ctccatttta aagccttggg ctcgccgtca
    15781 tccccgaccg ggagaatggc atcctcgggg aaatccttgt ccccttcgac cgaagtccag
    15841 acagcgtagt cgtgatttaa gcccttaatt gaggtgctga cgatttttgc gtaaaactct
    15901 ggctcggttt ttttgacgga tagagtgagg ctgatatttt ccttctgccc atcctgaagc
    15961 ttgtctatat taaatgaggt tttttcagcg gtgatattca ccctgccgac ataattgctt
    16021 tcaaatttgc cgtcgcttga gccggggcgg tttcctaaga ccgcaaatgt aatcgcggct
    16081 aaggacataa taagcgccac tgaaaccaaa atggcaacta gtcttttttt cgtatttagc
    16141 atttaaccgc ctccagagag cattttccgc tgattttaat atacaatgcc gtcgatacat
    16201 agtcaagcaa ataggttcca attttttcta tttacttagc tttctctctg cataaaaaat
    16261 ccgacagcat ctgtctgtat aaatcgtaac cgtaaattga gtttattagt ttaattaagc
    16321 tcgatgttga aagatgtgcg ggcagtccca aaaatttgag gaattcttct ctgagtttct
    16381 ttgagccgtc tttaccgcaa agcccgtcgg acattaaatc aaaggcagtg atttctttat
    16441 gagggctaag ctgcgcatta ccctcgtttt caattcccgt ccttttaagt gcgtttacaa
    16501 taatttcgtc tggaattccc tcaagcccca acttgccttc tgctgatggg gccgtttttc
    16561 ttttctcctt gccgaaaaca tcgggaatat aagcgtggag aaaagtgccc tttggaacga
    16621 tgcttgcgac aaaggctctt atcttaaagc ccgctgcgtc actatcggta aaaataataa
    16681 tgcccttttc ttcagcgatg cgcttaatta gtttttgcag ttccttgtct ttaaatactc
    16741 taaacccgtc ggtttcgata attaaggcat cgacaatacc gctgagcttt atcttatcgt
    16801 attttccttc aacaataata gccctgtcag tttttaacat aaaacacctt aatttatagt
    16861 aaggagcgac gccaccaaag actcaattac aataaagctg tccgctccgg ttgactttat
    16921 tttgctgtcc gcctcagtaa gattttccaa agcttttctg atgcttttca tgcctatttt
    16981 tcccgctgct ttcatggctt tttcgagtct gaacgccgtg tttttataat cataggcgga
    17041 aattatattg gaaagccctg ccctctcctt atcagcaacc tttgctctgt ataaatccgc
    17101 aaaataggaa gataaagtgc cgattatcat ttgcggagaa accttgtcca gctttaaagc
    17161 ggagagaatc cggtatgctt tgtcggaatt cttttctatt attgcggcgg agaggtcaaa
    17221 gattgaagcc tcaacacttt taaccgcgac tttatcaata atctccttag taatgccatc
    17281 tttgcccgca aaggcggcaa gctttgtcag ctcattcatt aagacgagca aatcgtcccc
    17341 aactatttca acaaggtacg cggccgcctc tttggtgatt gacgaacctc ttgactttgc
    17401 gccgttcatt ataagagtta cgattgaatt tgtctgacgc ttgcttaaac acgccgcaat
    17461 gcctgctttt gaaaaggcgc cgaccatatc tttaacagca gcgtttcgct tttcctcctc
    17521 cgcgccttct tccgctttta caataagagt gcaaaagtcg ggaatttcag atattaacct
    17581 tttaatatcc tctacagcgt ctttttttaa tgcggcgagt ttcatgccct taataaaaac
    17641 gcacgagcgc tcgctcataa gaggataagc ggtggcgcag tcccagactg aggagatgtc
    17701 catttcatcc gcctcaaatc catggagatt gaacgcgctg aatgcggggt caaccgctct
    17761 atctctgata aaagcggagt aatagtcctt taaataatca tcttccccgt atattaaaaa
    17821 gacattaaca tttgaaggct cttttaaata gtttttcagc cgggtttcat caaatagcgg
    17881 catgtcaatc ccccgtttta atgctaaagg ctttgttttt tatgctgatc tcaacacccg
    17941 aatcgctggc agttaagaga acaggatttt tgctgttttt ctcaaacttt ttttcgcttc
    18001 cgattttgta gctgtccgcg ctgataatta cccgctcgaa aaccttgttt cttaaagatt
    18061 cgggataggc tgaccttgca atcagaatat cagcgtcaga aaaactttca aatcccgtaa
    18121 aatcgcttga aggggtaaaa caaatcagaa ctgcctttcc tgcaaagctt attcgagcgc
    18181 agcttagtga atcgttatta atatattcta acacaaccga gccccaaagg acaatctttg
    18241 cggtgtcgct ttgctgtgag tcgatatttt tattgtgcat aaaaagcacg gggtcaacag
    18301 actttgaggc tattaaagtc tttggagtat gctcttttat cacctttgga gcaaaaccga
    18361 tttcagtttc tttgtcacgg ggaagtacga gaaggtccaa ggaatatata ttctccctgt
    18421 aaagcctgcc gtttatctcg taaggaacat tgtattcccc cccgcagcct attattgcgg
    18481 ctctgccgtt taaagtcaga attacagctg tggaattccc cgcatcgggc acatatattt
    18541 tgagtttatt aaaatccgaa aaatagctta tattcacgcc caagagcaaa accattattg
    18601 atgctaaaaa ggctcttagg atattccgca tatttttatt tttcttatca agcaaagaca
    18661 ggttcttttc tctgtctaag agttttaaga ataataagca tataggaaaa gctgaagccg
    18721 cagctgccaa aacgcctttg ctgatattta tataggcaaa gggcagcttt gcaaatagac
    18781 tattaatcaa aataaaacaa tccaaaagga aacttgcgat atagtaaagc gcgcttgata
    18841 aaaatttaat gggacttgag ataatcgcca gaccgcagaa aagcattgct gcctctgtgg
    18901 gcagtatgga tattatattg cttaaaggcg caatgaaaga aatgccgccg aaagccaaaa
    18961 cagaaacggg catggtaaac aacaccgccg caaggctaat acaaaaggcg gtgacaatgc
    19021 ccgacacaag ccttttaagg ctttttagtt taatcttaat tttgcctgca aattcgttag
    19081 tctttcccgc tgccagcaaa attcccaatg tggcgaaaaa agagagtagg aaggacaaat
    19141 ctgctgccgt ataaggatta ataaagcaaa taacaaaggc ggcaaagccg agggaattaa
    19201 tcggttcaga gtttttatta aaaatctcgc cgagatagaa aagggacagc ataatccctg
    19261 ccctgattgc cgcggaggaa aagcctgtaa cggcaaccac aaaaaacaca aatgcaatag
    19321 acacaattcc cgaaagggtt cttcttttca gccttgtcaa caactgatag gcaaacatgg
    19381 accataagct gacatgaaga cccgataccg aaagaatatg agcggcacca gtttttcgaa
    19441 cttcatttaa aagcccggag tctattcccg aggtatctcc cagcataatg cctgtcatca
    19501 agccttcggt ttcctcggga aaattagttt ttatgatgtc ttttatgtat tcccttgctt
    19561 tcgaaattga agtgaaaagc gtcttagttt ttgcgggctt tacagtgatt tctccatatg
    19621 tataggcgcc gaggaaaagg gaatcagctt tataggacct tttcgctgtg tcgcttccgc
    19681 cgagtttgta aaccgaatcc gccttgaatt tcaccatatc gttgacttcg gcatatatgg
    19741 gcgttctgct gtaaagcctt agattaactc tcttttcttc attgtctatc agctcggtct
    19801 ttataatata ataattcctg ccgccgcttt gctcgtaatt taccactctg ccgacaattt
    19861 ctttattgct cccgactgat tccagagcct tgttgtattt aagctcggca acggaaacag
    19921 tcagaagaat ggcagaggaa aagcataaaa acacagcggc aagagttctg agtctttcat
    19981 ttttaaagca cagcaatgtg aaggctaaag aaaacagcaa agaaacaaaa agaagcatta
    20041 ccgattgctt aaagggcata aagcaaccga taaagctcgc tgtaaaaaat gaaaaaccta
    20101 tcagcgcaaa gggcctgttt atcctgttct cccctttcgc agtttgcttt taaaggtaaa
    20161 ggttttcaaa aataattgca ataaaaagtt aaactctgat tcaatcgaat aacggcagcg
    20221 gctcaaggaa aataatgtcc tgcctctcgg ctgcctcgcc ttcggcaagc actgcggcgc
    20281 atgcggcaat ttcggcgccg aaacgctttg caagttcgat taccgcattt aatgaattgc
    20341 ctgtgcttat aacatcgtcc acaatcagaa cctttttgcc acgcattaac tctacgtcac
    20401 tttgatcaat ccaaagctgc tgattgtttt cagtggttat ggatttaacc tcgactctta
    20461 cggggtcgtg catatagagc tttttgccct ttcttgcgac aagatagggc ttacccgact
    20521 gccttgccat ctcataagca aggggtatgc tcttagcttc gggagtgagg ataatatcaa
    20581 actcggggca ctttgccaaa agctcttttg cggcagcaat agtcagctcc acatcattga
    20641 acattataaa agccgcgatt gaaagattat cgtttattct gcagataggc agctctcttt
    20701 caagccctgc tatagtcatt ttgtattttt ccatactaag tcctctctga gaatattaat
    20761 gctgtgccgg cttatttacc ggcacagctt atttttatta gcccggaggc caggaaaagg
    20821 aacggccgcc aagcaaatgg aagtgcagat gcattacaga ctgaccggca gaggcgccgc
    20881 agttggttac gactctaaag ccgctctctt taactcccat ctgttcggca attttgggga
    20941 ttacctcaaa aatcttggca acatatgcgc tgttttcccc gtttatttcg gcagcagaaa
    21001 cgatatgctc cttggggatt aaaagaatat ggacaggcgc tctgggggat atgtcctcaa
    21061 acgcaaggac atgttcgtct tcatagacct tttttgaagg gatttcccct gcaattattt
    21121 tgcaaaaaat gcagtccacg ctcaaacctc ctgtcattta tttattaagc atagccttga
    21181 gagcttcttc cgcagaatcc aaagcctcat caacttttga aatatccttg ccgcctgcca
    21241 tggcgctttc gggctttccg cctcccctgc cgcctgccac agccgcgaca gcttttacaa
    21301 ggttgcccgc atgtgctcct gccttaaggg ctgcgcttcc gcaaactgcg cagaaggtgc
    21361 aggtaccttt ttcggactga gttgccgcga ttacagcgac cacatcgtca ccgctttcct
    21421 tgattctgtc agcggcggct cttgcttcgt ctgcggctgc ctcgccgata ttagcggtta
    21481 tgagcttaat accgtcgatt tcagcgcgga ctttaagctc tgagcttaat ttcgcggcag
    21541 caatctcagc ctttaatgct gcaatctcct tatccttttg tttaagctcc ttattgagct
    21601 gagcggcctt ttcggttatc tcataaacat cgccgcactt taaaatctcg gcggtttcgt
    21661 aaagcactgc ggtattcttt ctaatgtatt caagcaggtt aagtcccgta agagcttcga
    21721 ttctgcgaac gcctgcggct acggaggttt cggaaattat cttaaacata ccgatttcgc
    21781 ccgtgttgga aacatgggtt ccgccgcaga gctcggatga gaaatcgcct gccgatacga
    21841 ctcttacaac atcggagtat ttttcgccaa agagagcgac agcgcccttt gcctttgcct
    21901 catcaagtcc cataagctcg gttttgacat cctgagctga cattattgct tggtttacca
    21961 tttcctcaac tttgtttatt tcttcggagg tcaaagcgga aaaatgggta aagtcaaagc
    22021 gcataagctc gggagttaca ctctgaccag cctgctcgac atgctgaccc aaaacttttt
    22081 taagcgcggc gtgaaggaga tgggcagaag tgtgattttt cttaacctcc tctctggact
    22141 ttttgcagac gcttgcactg acttttgcgc cagtgaaaac agannnnnnn nnnagaaaca
    22201 ccctcagaaa tatatccctt atggatataa acattctttg cggtcttaat tgtatcggta
    22261 acggtgaact caaagccgtc gcctgtgatt tttccaatat cgccgcactg accgccgccc
    22321 aatgcgtaga agggagtttt ctcaagaacg attgcggttt cttctgagtc tttctcgatt
    22381 gtctccacta tctcgccgtt agagagaagg gcaataattt tggtatctgc cgaatcggtg
    22441 tcgtatccca caaactcggt ttcgggatag tcctttaatg cggctgcgga atccttccaa
    22501 gcgtcggatc cgctgaactt tcttgcctcc cttgccttct gcctgtgaag ctgcataagc
    22561 tcctcaaacc tagctttgtc aagggtcttg ccctgctcgt taacgatttc ttcagttagg
    22621 tcaatcggga aaccgaaagt gtcggagagc ttaaaggcgt cgtcgccgct gaggctgtcc
    22681 ttgccctcta gcatttcaaa gagcagtttc atgccggcgt ctattgtctt taagaagttt
    22741 tcctcttcat gggtgattat attaatgatg aaatcgcgct tttcggctaa atccggatat
    22801 gcgccggagc tgaacttgat tacgctttca gcgacttcg
//
LOCUS       DAONVQ010000014        22470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_1642_length_22470_cov_4.636895, whole genome shotgun sequence.
ACCESSION   DAONVQ010000014 DAONVQ010000000
VERSION     DAONVQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 22470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22470
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1642_length_22470_cov_4.636895"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1783
                     /locus_tag="PLD98_01910"
     CDS             137..1783
                     /locus_tag="PLD98_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05038.1"
                     /translation="MKLKVKALITILITLLIFPLNLNAKADDDYSWVGAWHTSPVKTN
                     LTVADRDITPVLAYTTVRSVITVSLGGDNLRLCLTNKYGNKAAYINRVYVAKTDPNNE
                     SSIIGDLYTVRFGGSKTLTLGREFVYSDPIDMKVEPLDKISISIYFTTPTTVSTAGLT
                     GGVTYIAAANQVKEKNLIAPSKLRLDLDGASYYPIPFVTSVDVDNPNAYSVVMIGDSS
                     LANRVPNLLSEKLINGGTRNIGVLQSSIAANRLLTGGYSGGDGGSIQGDSAVSRFDDD
                     ALKLSGVKKIFVKVGANDILHPKLKSFKGSVPESTAADIIRGYESLISKAVAKGIEIY
                     FFGITPFKGYGGSDLQRYSDAEKTAQEVNTWLKNNRRINGYIDIDKLRDPADSAKLRT
                     ALTTDGLNLNNLGQIALTDLIPLEVFGIDASRYKTAAELNRVDPYKGSQSPTAPTKPA
                     EESTTRRGEEPTREDEITTEPVIEDFEPIEEVTEDIPVNSPGKNNKNPYANLHEIPGA
                     YRKMSKKQRSGIIFFTSLAVALTAFGSMYYVNRKKMWEST"
     gene            complement(1815..2765)
                     /locus_tag="PLD98_01915"
     CDS             complement(1815..2765)
                     /locus_tag="PLD98_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05039.1"
                     /translation="MDLNALVLDYCDLTINGEDKGKNIPIVDVQEIACSYGEPVNLEA
                     VFSFNVKAVPEGELYLVCETPELFDIEINGEKLAQKDCGCYRDKSFRKLDISGLLRKG
                     RNTVALKTLFKQSPETYENISKAGGFESYRNKLSYDMEIDAVYIIGNFGVETDGEFTE
                     IPRNALSFKGSFSVAEPPKTVKCGEIATQKLPFFNGRIKYRKKIELKAGETENRYIEF
                     AAKPDTVTRVWANGKLAGEILWRPYRAELGGLLKEGINIIEIETVGTLRNLLGPFHLK
                     EESYGLGPHTFMHISNIWRKGLNPAWDDAYVFLKTGLFFK"
     gene            complement(2795..4054)
                     /locus_tag="PLD98_01920"
     CDS             complement(2795..4054)
                     /locus_tag="PLD98_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05040.1"
                     /translation="MRTISQKEYTSGARKKGVLLTGHRVLEETLGDQVTSNGAVMPNY
                     EFFHIPGMDRLVRHANNCLTPVQLVSAANQLGKKQILTETFACCGWGLSFEEMKWIYE
                     WQMGRGVNLMCEHLSPYSMAGIRKRDYPAFHFYQSPWWDDYKKFLDYSSRIGMLIAEG
                     KPDYKVLVLHTQSTAWLLYNDKKSGYAYIAIYNEHLMSVVKALEHSQIPYHFGDDRII
                     RRHGKIEGDTFRIGTQSYKTVIVPPSMNINSNTLKMLYEFKENGGRIIWCGEVPSMVD
                     GEKRDMAKALSDTCAFADEPSELISLIPDYAKTVRVKTKDGSDARGMTCLIREYPESK
                     CRLIYIVNTYAQGSDIDIILKAEDLRLYDALNDEIYSVDFEKRGDKIVFSAFIEEKGS
                     IVLFAYDERQGITLRNTGLYKMQLPLP"
     gene            complement(3975..4433)
                     /locus_tag="PLD98_01925"
     CDS             complement(3975..4433)
                     /locus_tag="PLD98_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05041.1"
                     /translation="MEVTSKPKETETTIRNIEHGGENLHFYYEVNEFYVDTLNAKVTD
                     KFIEAIYEEYKLKLGEDFSRLKGFFTDEPQVSRNGIPWSFILPDEYKNAYNEDLLSVL
                     PDLFLDTDTSMQTRFRFWHLVCELFADNFAKRIYEWCEKKRGTAYGSQGS"
     gene            complement(4424..4576)
                     /locus_tag="PLD98_01930"
     CDS             complement(4424..4576)
                     /locus_tag="PLD98_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05042.1"
                     /translation="MENVKASIDEGEKYNMGAWGYDENGWPSGFGDGRVNELGIEYQQ
                     KYSAWK"
     gene            complement(4577..4768)
                     /locus_tag="PLD98_01935"
     CDS             complement(4577..4768)
                     /locus_tag="PLD98_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05043.1"
                     /translation="MKDLRNLLHNPPNEYRPIPFWAWNARLEAEESRRQVRELFEKGN
                     GGFFMHARGGLTTPFMGDE"
     gene            complement(4829..5107)
                     /locus_tag="PLD98_01940"
     CDS             complement(4829..5107)
                     /locus_tag="PLD98_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HQA05044.1"
                     /translation="MDILNAVLLLSGLGLVLGLVLSIVDTLVSVPSDDDKAKEIEEAL
                     PGYNCGVCGYPGCQGYAAALASGETDDISLCIPGKKATVEKLAEILNK"
     gene            complement(5135..5725)
                     /locus_tag="PLD98_01945"
     CDS             complement(5135..5725)
                     /locus_tag="PLD98_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HQA05045.1"
                     /translation="MKTYIALLVMGILTQNFVLSRFLGICPFLGVSRQTNTAVGMGLA
                     VTGVMTLAAIISHPFNVLLNNMGLEYLQTTVFILIIASLVQILEVFIKRYIKALYSAF
                     GIYLPLITTNCAVLGIVIINAQEAYTFPQSIVSALGAGLGFLLAIFMFSIVRSKINEA
                     HVPPSFRGMPITLIAASIVSLSFMGFTGIAQGLGLA"
     gene            complement(5725..6453)
                     /locus_tag="PLD98_01950"
     CDS             complement(5725..6453)
                     /locus_tag="PLD98_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HQA05046.1"
                     /translation="MAKTKVSPLETFSKGFVKENPVLRLVLGTCPALAVSNTMVSAIG
                     MGIATIMVLVMTNTIISALKKVIPSKVRIPAYIVVISTMVTVVQMLVKAFLPVIYDAL
                     GVYLPLITVNCIILGRAESFASKNSVALSILDGLGMGIGFLAALFSISLIREFVGGGT
                     ILSGIQSLLPFLSGSSFNGVDVASAVSQYTALEPIKIFILPAGGFMVFGFLMALVNRI
                     NHKKGKPSVELGCGSCKACDKEAK"
     gene            complement(6455..7042)
                     /locus_tag="PLD98_01955"
     CDS             complement(6455..7042)
                     /locus_tag="PLD98_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HQA05047.1"
                     /translation="MQKLKGILLPTLSLLMICLVCAGLLAFTNGFTKEYIEKAEKEQI
                     LNMLSSVIESDRFSEQKEYSTGRGEPYPYYDAFDKDGNRIGRVLITSTNGYGGILRLV
                     TGIDAEGKVTGLKILELHETPGLGSKVNSESFLNQFKGISRELILSKAGNTGASLGQV
                     GLIDAISGATVSSGAVSSCVNTALEVNALIEKGAN"
     gene            complement(7029..8111)
                     /locus_tag="PLD98_01960"
     CDS             complement(7029..8111)
                     /locus_tag="PLD98_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HQA05048.1"
                     /translation="MENAKNPAISEDKSANSVQDNNKDNLSELKLTVSPHIKSSLTTK
                     GVMLDVIIALIPTLAAGAWIQGPRAFLIAFTGVLSSVLAEYLYNRFMKKPNTIGDLSA
                     AVTGMLIALNMPVTIPLWMVVVGSVFAIVIVKQLFGGLGRNFANPALTARIFMLLSFP
                     AAMNNFVMPFQWKSSSEIVTTATPLYTLYEAFKSGSPDSFLTAAKDFPSLTSLFLGQM
                     QGAIGEISALALLLGFIYLLYRGVIRLSIPVSFVGTVAVVMLIAGKGSLNFVAGQILS
                     GGLLLGAIFMATDYTTSPMSIKGKIIFGIGCGLITCFIRLFGSLHEGVSYAILIMNIF
                     SPFIDRLTRPRPFGQTKKGGKAHAKA"
     gene            complement(8080..9471)
                     /gene="rsxC"
                     /locus_tag="PLD98_01965"
     CDS             complement(8080..9471)
                     /gene="rsxC"
                     /locus_tag="PLD98_01965"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HQA05049.1"
                     /translation="MKTAKDIFLKPVSRIKGGVLVKDNKTTFDSVPKSLSVSRVVIPL
                     LQHCTKPASAIVAVGDKVKKGQLIGDAGDATARIHASVSGTVTDISVRKIAPKEKAVC
                     VTIESDGNDEWLETIAPPTVTDAESLIDAVFNAGLAGMGGAGFPTHIKLRSVYEAKAK
                     TLIINSIECEPYITVDYRACIDFGDDIIRCIEALIKHIGFERVLIAEEDKKEKGDEQA
                     LIERIEKSEYKDIIKIVRLKPKYATGAEKMLIKALLGISLPEGKLPSDASCMVMNTST
                     AAAFGNYLLTGRPLVSRCITVAGSAIKEPANIIAPLGALISDIIDFVGGFEAKPYKLI
                     IGGPMMGHAVDSTDYPVTKTTNGILAFAEKMARVKTETSCIRCGKCAEVCPMRIVPTD
                     IYKAATDYGKPDINELVALNAQACILCSCCAYICPAGIPLVKGLDIGKRLVAKAKAKG
                     AGNNGKCKESCDI"
     gene            complement(9530..10090)
                     /locus_tag="PLD98_01970"
     CDS             complement(9530..10090)
                     /locus_tag="PLD98_01970"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HQA05050.1"
                     /translation="MKGQGKKGKKNYPLLISLYGILSALAICLSYLENLVPQFPFFPP
                     GAKLGMSNVVILLAILFLGVKGSLFIFIAKSVFALLTRGTFAFLIGFAGGAGAFVISA
                     IILNIGVLYKKGHISLAGVSMLSAAAFNILQLAAAVYVTGTDFFAAYLPFMLFASVVS
                     GLITGIVLNLSAPAIEKYASLNRLFI"
     gene            complement(10180..10578)
                     /locus_tag="PLD98_01975"
     CDS             complement(10180..10578)
                     /locus_tag="PLD98_01975"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HQA05051.1"
                     /translation="MKLHKAGIKKGDIAITLFLISISLLSFVLAPAGKGGGERFAVLY
                     RKGEEIERISLDRIEEPIEKTYEFDGSKAVIKYEKGSAAFIYSDCHDKLCIQRGYISK
                     QADASVCLPLSLVLIIEGGETDADFDAVVY"
     gene            complement(10590..11660)
                     /locus_tag="PLD98_01980"
     CDS             complement(10590..11660)
                     /locus_tag="PLD98_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQA05052.1"
                     /translation="MKNSSNKRNKNLSAFAFLAVVLLLMLIAGDIKGGAQSVNKSSSA
                     MGAAVFITLYGGTEDNADAMISAIKELDEKCLSPTVEGSDIFRLNENAGAELEISQKT
                     AEILKTSLEICAKSDSALNVALGKLVKLWGIDTDNPRVPAEEEIKTALETADLSKIIL
                     NGNKAKIGEGQLVDLGAIGKGAACDIAAETAKKLGVKAASISVGGSVTFVGLKPANGV
                     FSRFSKNEGVQWSIGIRDPFGPVSETFASVKTGPASVSTSGNYEKYFEKDGVRYHHIF
                     SPVTGYPSDSGLVSVTVFSDSGLLSDALSTICFVLGYEKSLPVLKEYSAEAVFVFPDK
                     TVKATDGIKDSITIKNSEFTLV"
     gene            complement(11650..13164)
                     /locus_tag="PLD98_01985"
     CDS             complement(11650..13164)
                     /locus_tag="PLD98_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HQA05053.1"
                     /translation="MFKEPIRNHPSVIFEKLAAFWGFIAVMLLSEGFSQFLEGKSRIF
                     SVTYWRGFFYTLKSGDFSVLLSGAGLFLICVIVIVISFYVWRKTFFSIRDSVLVVDKK
                     TLFKKVSKLPASNISTVNIERSLIERLIGTAKVKVDINSSTTAEQTDFTFVLKLPLAE
                     LFKEELIKAKAGEENTQTAVPLISAFEESRADTIIKFGFSEVIRHQLLSIPFAQGLLA
                     LLTFLPVFLEDGRYSAGGTDQRIFLLWLLLIGAVFSVVKSVFKNWNFTLMKDGGNIYI
                     KKGLIKTSSIAFGADKINGVIIKQSLLARIFGLYSIEAAVIGLTTQQEKTPVLSLLTT
                     KSEAERVLKLCAPDYMTSSQLIPASKKSLVPYMIAAVFSSGVSAPLIFVPIQKTWISF
                     IAIAIFSFILAYMSYRTKAIAFDERLVKIVSGILLRKTVLINFSDIQKIDIKSDFIVK
                     RLGIARAAVSILSASAQRNHLTGYFDRALLDEIADRTVKAKDALSTVRRFSYEK"
     gene            complement(13157..13666)
                     /locus_tag="PLD98_01990"
     CDS             complement(13157..13666)
                     /locus_tag="PLD98_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HQA05054.1"
                     /translation="MDNKQSGEFMGLTKQALGVLRVTAGIQCLAVLALSLLLFKLPIR
                     PVFSVLMILVILILCLVYFFKITEFRHRRYKYRISDDRIDIIEGVFFVSHTIVPVDRI
                     HQVEIAFGPIDARFGVAKVIVTTAGSRAVFRFLEKERADAVAEKLNKMIKERLAAAQK
                     GEGTGEGDV"
     gene            13966..14313
                     /locus_tag="PLD98_01995"
     CDS             13966..14313
                     /locus_tag="PLD98_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HQA05055.1"
                     /translation="MSSVSEKMPDGKLYISNDVIATIAYNAAKEVEGFAGLSKLSPSS
                     KSLFKANKHSIEIKSGQTGIEISIAVDLKSDASVQNTVKLIQSNIKNAVQNMTGNVVK
                     RVNVTVNDIVFED"
     gene            14336..14851
                     /gene="nusB"
                     /locus_tag="PLD98_02000"
     CDS             14336..14851
                     /gene="nusB"
                     /locus_tag="PLD98_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQA05056.1"
                     /translation="MGRREEREQAFNIVFSSLFQPEEPLEDIVKAETEAEAYVPSDYS
                     RRLIKLISENLEEIDRIIEKRTVRWKKERLSKVALSVLRISLCEILYFDEIPVSVSVN
                     EAVELAKKYSIPEDAAFVNGLLGSYIREREGQNAETEAKETEEQSVKEEIEALAAESE
                     AEKQAEMEVTS"
     gene            14856..15803
                     /locus_tag="PLD98_02005"
     CDS             14856..15803
                     /locus_tag="PLD98_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M22"
                     /protein_id="HQA05057.1"
                     /translation="MDIYLGIDTSNYKTSVAVYEPESDSIIADERRFLTVKEGELGLR
                     QSEALFQHVKELPALIEKAFSGLKNFTLRASAASVSPTNREGSYMPCFLAGESAARAI
                     AAANSVPFYRTNHQAGHVMAALHSAKALSLLDEGFVAVHLSGGTTDVLEVKKDPESII
                     EIKPLSASLDLKAGQVIDRVGKMLGIPFPAGEELEKLALKSDKEFKVKPYFKDGEASF
                     SGLQNQCEQKLKKGESKEDIAKYCISYVTEAIILMAQSALQSTGMKKALFSGGVTANS
                     YLRKKALERLDCVFGSPEMSGDNAAGIAVLCCLLHNPQK"
     gene            15814..17025
                     /gene="xseA"
                     /locus_tag="PLD98_02010"
     CDS             15814..17025
                     /gene="xseA"
                     /locus_tag="PLD98_02010"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQA05058.1"
                     /translation="MAQEYVLSVSQLNFYIKSIIDADRKLYDLYVSGEISNFTRHFRT
                     GHLYFTLKDKSSAIKVVMFSKAADRLRFTPEDGMQVIVRGRVSVFERDGTYQLYAESI
                     EAQSVGELAAALERLKQKLKSEGLFDESRKQPIPRFPKRVGVVTSASSAAIQDILSIL
                     ERRWPLSEVVFCPVSVEGLKAAEEISNAIKEFNDKKAADVLIVGRGGGSVEELWTFND
                     ERIARAVAASRIPVISAVGHESDYTLIDFVADLRAPTPSAAAELAVPERAELLHRIRL
                     LEQRLKNSVDEIINTKTYRLRLLENKLSSREPTAMIENRIQLIDNLIQRLNKTADIYL
                     SNKQNRLIKNISMLDALSPLKNLQRGYAIAKSEKGKIIKSVSAVKVGDKIDVNLTDGA
                     LKCNILEMKEL"
     gene            17030..17233
                     /gene="xseB"
                     /locus_tag="PLD98_02015"
     CDS             17030..17233
                     /gene="xseB"
                     /locus_tag="PLD98_02015"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HQA05059.1"
                     /translation="MPEFSYEKSIKRLEEIISRLEQGDIELEEAVNLFDEGTKLAVSC
                     RKSLEEAKQKITLLKNAGSAESN"
     gene            17247..18107
                     /locus_tag="PLD98_02020"
     CDS             17247..18107
                     /locus_tag="PLD98_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQA05060.1"
                     /translation="MVKEVLNENLKLINPFLKERISGANLSTAAENLVLDAAAYSLSS
                     GGKRVRPVLTLEFCKLCGGDKKDALHFAAAVEFIHTYSLIHDDLPCMDDDDVRRGQPS
                     CHVKFGEAVALLAGDALLTLAFETLADAPLKCEQIVKAVKALSSLAGVEGMIGGQSID
                     IDSENKKLTVEQLEKMDLMKTGALIKAACVLGCIAADASEEEIQSAGTFAESLGLAFQ
                     IIDDILDGDEQGEDEKLNKATYVSVLGMEGARALAEEHTRNALSALAPFGERAQFLRE
                     FAITLLERSV"
     gene            18141..20030
                     /gene="dxs"
                     /locus_tag="PLD98_02025"
     CDS             18141..20030
                     /gene="dxs"
                     /locus_tag="PLD98_02025"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQA05061.1"
                     /translation="MEEFKLLKNISGPADVKKLKKEELPQLADEIRQVLINTVSKNGG
                     HLASNLGVVELSIALHRVFCSPYDKIVWDVGHQCYVHKLLTGRFDRFDTIRTEGGLSG
                     FCRPDESEHDTFYSGHSSTSISAAYGIAAANKLKGNKNYTIAVIGDGSFTGGLVYEAL
                     NNAGRSKTRLIVILNDNDMSISRNVGALARYFAAIKARPGYFRLKAKTEQLINKIPFV
                     GKKLSITLFRIKTYIKNMIYKSTIFEDFGFRYMGPINGHNINQLIDALEGAKAANHPV
                     VLHINTVKGKGYDHAEKSPEVFHGISQFDINTGEPLSAGTNFSAEFGRFLIDVAEKDR
                     RICAITAAMSVGTGLDGFAARFPDRFFDVGIAEAHAVTFASGLAKAGMVPVFAVYSSF
                     LQRAYDQLIHDGALQRQKMIIAVDRAGFVGEDGETHQGIFDVAFLNSIPNVTVYSPST
                     YAEMRRCFINAFYHQDGLAAVRYARGTECILPDDFEPTFSDYEVYGDKEAYTVIVTYG
                     RLFGFAAAAVRRLKEQGIPVMTLKLTKIKPIDKDAVTILLGKKKIFFFEEGVKSGSVG
                     ERLALILKEQGYEGEYTLTAVNDSFVPHAPVKSLLKRYKLDTDGMCEIITQSFQKPAA
                     IGFEP"
     gene            20043..20855
                     /locus_tag="PLD98_02030"
     CDS             20043..20855
                     /locus_tag="PLD98_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HQA05062.1"
                     /translation="MSELQRLDTEIVKRGLYDSRQKAAAAIAEGRVKVNGKTITKSSY
                     KVSPADSIEIEKGVSDEYVSRGALKLKKALDVFNISVRGLDCLDIGSSTGGFTDCLLK
                     NGAARVIAVDVGTNQLHDTLRQDKRVFVYENTDIRDFAKETLPFNVRFVSCDVSFISL
                     VLILPSVYNLLEDKGQAVLLIKPQFEAGRENIGKKGIVKDRKIHTEIIHKIYRSAKDL
                     GFFVCGLDFSPIKGGDGNIEYLIHLKKGDNEGEITDFDALIERTVNFAQNLN"
     gene            20872..21729
                     /locus_tag="PLD98_02035"
     CDS             20872..21729
                     /locus_tag="PLD98_02035"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HQA05063.1"
                     /translation="MLAAIMPNLTRKNAFDVTVAACKKLDSAGIAYAFPEEYRGDFPV
                     LTNASFYKEDRLFKDCDLIIAVGGDGSVMHSAKHAALNSKPLFGINAGNLAFMASLES
                     DELDYLERLKTGDFRISRRMLLEYELYDEEKLIKKDYCINDIVFARGSQIKLSHINVE
                     CNGREIGKYSADGIILSTPNGSTAYSLAAGGPVVDPDIQCIILTPVCPHSLFARSVIF
                     CRDSVFTISPAEEENSPLVISCDGEEAMPLNKSGKCVIKCADIDVGFIIFKEDTFTDV
                     LYNKLATNS"
     gene            21782..22234
                     /gene="argR"
                     /locus_tag="PLD98_02040"
     CDS             21782..22234
                     /gene="argR"
                     /locus_tag="PLD98_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HQA05064.1"
                     /translation="MKKARHELILKLIEENDISTQEELLEKLKERGIKVTQATVSRDI
                     KELRLIKQPSARGEYRYTCARNTTDDEDARFHTVFASSVISVDYAQNICVLKCHTGTA
                     QAACAVFDEMKWHDTVGTLAGDDTIFLLCRTEAAAKNICETIAKQLDI"
     gene            22267..>22470
                     /locus_tag="PLD98_02045"
     CDS             22267..>22470
                     /locus_tag="PLD98_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA05065.1"
                     /translation="MLATLNIENIAVIEKANIDFSDGFICLTGETGAGKSIIIDSINA
                     VMGEKTSRELIRTGAETAMVSALF"
BASE COUNT         6147 a         5444 c         5364 g         5515 t
ORIGIN      
        1 ccgacgcgag aaaaatcctc agattctacg caaagctgga accaatggat taaaaaacgg
       61 cacggcaaaa aacgccgtgc tgtaatatta ctgcctgtat tgcggcaccc aagaaaatgg
      121 atgcggggga gagaatttga aacttaaggt aaaggcttta attactatac tcataacgct
      181 tcttatattc cccttgaact taaacgccaa ggcggacgac gactactcat gggtgggagc
      241 atggcataca agcccggtca aaacaaatct caccgtcgcg gacagggata ttacccccgt
      301 actcgcttac accacagtca gaagcgtcat caccgtttct ttgggcggcg acaatctgcg
      361 tctttgcctt actaacaagt acggaaacaa agccgcgtac attaacaggg tctatgtcgc
      421 caaaaccgac ccgaataacg aaagctccat aatcggcgac ctttataccg tcagattcgg
      481 cggcagcaaa accttaactt tagggagaga atttgtttat tccgacccca ttgatatgaa
      541 ggtggagccc cttgacaaaa taagcatttc catttacttt accaccccga ctaccgtcag
      601 tacagcgggt cttacaggcg gagttacata tatcgccgct gcaaatcagg taaaagagaa
      661 aaatcttatt gccccctcaa agctcagact cgaccttgac ggcgcgtcat actatcccat
      721 accctttgta acaagcgttg atgtggataa cccaaacgcc tattccgttg tgatgatcgg
      781 cgactcatcc cttgcaaaca gggtgccgaa cctcctttcc gaaaaactca taaacggcgg
      841 cacaaggaat atcggagttc tgcagtcttc aatagccgcg aacaggctgc ttactggcgg
      901 ctacagcggc ggcgacggcg gcagcataca aggggattcg gcagtaagcc gctttgacga
      961 cgacgccctt aaactctcgg gcgttaaaaa gattttcgta aaagtaggag ccaacgacat
     1021 actgcacccg aagctgaaga gctttaaagg cagcgtgccg gaatccacag ccgcagacat
     1081 aataaggggc tatgagagcc ttatttccaa agccgtggca aagggcatag agatttactt
     1141 tttcggcata acccccttta aaggctacgg cggcagcgac ttgcagcgtt acagcgacgc
     1201 tgaaaaaacc gctcaggaag tcaacacttg gcttaaaaac aacagaagaa taaacggata
     1261 cattgatatt gacaagctgc gggacccggc ggattccgca aagctcagaa ccgccctcac
     1321 aaccgatggg ctgaatctaa ataacctcgg tcaaatcgct cttacggatt taattcctct
     1381 tgaggttttc ggaatagacg cctcaagata caaaaccgct gctgaactta atagagtaga
     1441 cccttacaaa ggctcacaga gccccaccgc ccccacaaaa cccgctgagg agagcaccac
     1501 aaggagaggg gaggagccaa cgagagagga tgaaatcaca acagagcccg taatcgagga
     1561 ctttgagccg attgaggaag taaccgagga tatccctgta aacagccccg gaaaaaacaa
     1621 taaaaacccc tacgcaaatc ttcacgaaat ccccggcgcg tacagaaaaa tgagcaaaaa
     1681 gcaaaggtcg ggaatcatct tctttacttc cctcgcggtt gctttaaccg ctttcggcag
     1741 tatgtattat gttaatagaa aaaagatgtg ggaatcgaca taaaaaagaa agggcagagt
     1801 aatctgcccc ttttttattt aaagaaaagc cctgttttaa ggaaaacata agcgtcgtcc
     1861 catgcgggat tcagtccctt gcgccagata ttcgagatat gcataaaggt atgcgggcca
     1921 agtccgtagg attcctcttt cagatggaag ggacccaaaa ggtttctcag cgtaccgacg
     1981 gtttcaattt cgattatgtt tataccttct tttaaaagcc cgccaagctc tgcgcggtaa
     2041 ggtctccaaa gaatttcgcc cgcaagcttg ccgtttgccc atacccttgt tacagtgtct
     2101 ggctttgcgg cgaattcaat atacctgttt tcggtctctc ccgcttttag ctctattttc
     2161 tttctgtact ttattctgcc gttaaagaag gggagttttt gcgttgcgat ttcaccgcat
     2221 ttaaccgttt tcggcggctc tgcaacgctg aaagagcctt tgaagctcaa agcgtttctc
     2281 gggatttctg taaactctcc gtccgtctcc acgccgaaat tgccgattat atagacggcg
     2341 tcaatctcca tatcgtagct gagcttgttg cggtagctct caaagccgcc cgccttggat
     2401 atattctcat aggtttcggg cgactgctta aatagggttt taagggcgac ggtattcctt
     2461 ccctttctca gaagccccga aatatccagc tttctaaagc tcttgtccct atagcagccg
     2521 cagtcttttt gcgcgagttt ctccccgttt atctcaatgt caaaaagctc cggagtctca
     2581 cacacaagat acagctcgcc ctcgggcaca gccttaacat taaagctgaa aacagcctca
     2641 aggtttacgg gctcgccgta tgagcatgcg atttcctgaa catccacaat cgggatattt
     2701 ttgcccttat cttcaccgtt aattgtaagg tcgcagtagt ccagaacaag ggcattcagg
     2761 tccatttcag caatttccca ctcaccgttg agctttaagg gagcggaagt tgcattttgt
     2821 aaagccctgt atttcggagc gttatcccct gtctttcgtc ataagcaaaa agaacaatcg
     2881 aacccttttc ttcaataaat gcgctgaaaa ctattttgtc gcctctcttt tcaaagtcca
     2941 cggagtaaat ctcgtcattc agcgcgtcat aaagccttaa atcctcggct ttaagaatga
     3001 tgtcaatatc gctgccctgg gcgtaggtgt ttacgatata aataagcctg cactttgact
     3061 cgggatactc cctaataagg caggtcatgc cccttgcgtc gctgccgtct ttggttttta
     3121 ctctaacggt tttcgcatag tcggggataa gagagataag ctcagaaggc tcatccgcaa
     3181 aagcgcaagt gtcggagagg gcttttgcca tatctcgctt ttcgccgtca accattgacg
     3241 gaacctcgcc gcaccaaata atccgcccgc cgttttcttt gaactcatag agcattttga
     3301 gggtgttgga atttatattc atcgacggcg gaacgattac tgtcttatag ctctgagtgc
     3361 cgattctaaa agtatctcct tcaatttttc cgtgacgcct gataattctg tcgtcgccga
     3421 aatgataggg aatctgcgag tgctcaagag ccttaacaac cgacatcaga tgctcgttat
     3481 atatagcaat gtacgcatag ccgctctttt tgtcgttgta taaaagccat gcggtcgact
     3541 gagtatgcag cacaagcact ttgtagtcgg gcttgccctc ggcaatcagc attccgattc
     3601 tgcttgagta gtcaaggaat tttttatagt cgtcccacca ggggctttga taaaagtgga
     3661 aggcggggta gtccctcttt ctgattcccg ccatgctgta tgggctcagg tgctcgcaca
     3721 taagatttac gcccctgccc atctgccatt cgtaaatcca tttcatttcc tcaaagctga
     3781 gtccccagcc gcagcaggca aaggtctcgg tgagtatctg ctttttgcca agctgattcg
     3841 ccgccgatac cagctgaacg ggggtaaggc agttgttcgc gtgtctgaca agccggtcca
     3901 tgccggggat gtggaaaaac tcgtagttgg gcattacagc tccgtttgat gtgacctggt
     3961 cgcccagagt ttcctcaaga accctgtgac ccgtaagcag tacccctttt tttctcgcac
     4021 cactcgtata ttctttttgc gaaattgtcc gcaaacagct cgcatacaag atgccagaac
     4081 ctaaagcggg tttgcataga tgtatccgta tcaaggaaca aatcgggaag gacgctcagc
     4141 aaatcctcgt tatatgcatt tttatactca tcgggcagaa tgaaggacca gggaattccg
     4201 tttcttgaaa cctgaggctc atcggtaaaa aagcctttaa ggcggctgaa atcctcgccc
     4261 agcttcagtt tatactcctc ataaatggcc tcaatgaact tgtccgtcac tttggcgttt
     4321 aaagtatcca cataaaactc gtttacctca tagtaaaaat gcagattctc tccgccatgc
     4381 tctatgttgc gaatcgtggt ttccgtttcc ttaggtttgc ttgtcacttc catgcggagt
     4441 atttctgctg atactctatt ccaagctcat ttaccctgcc gtcgccaaag ccggagggcc
     4501 agccgttttc gtcatagccc catgcgccca tattgtattt ttcgccctca tcaatggacg
     4561 ccttgacatt ttccattcac tcatcgccca taaagggggt ggtaagtccg cctcttgcgt
     4621 gcataaaaaa gccgccgttg cccttttcga aaagctctct gacctgacgc cttgactcct
     4681 cagcctcaag ccttgcgttc caagcccaga agggtatggg gcggtattca ttcgggggat
     4741 tatgtaataa attccttaaa tccttcatgc gcacctatcc tttttgatta taacaagaca
     4801 gacagcggta cacactgtct gtcgcatttt acttattaag tatttctgcc agcttttcta
     4861 ccgtagcctt tttgccggga atgcaaaggc tgatatcatc ggtttcaccc gacgcaagag
     4921 cggcggcata gccctgacag ccgggatatc cgcacacgcc gcagttgtag ccgggcagag
     4981 cctcctcgat ttcctttgcc ttgtcatcgt cagatggtac ggaaacgagg gtatccacta
     5041 ttgacagaac cagtcccaag acaagtccca gaccggacag caataaaaca gcatttaaaa
     5101 tatccaaaca atcacgcctc ttatatttag atttttacgc aaggccaaga ccctgagcta
     5161 tgcccgtaaa gcccatgaag gaaagtgaca cgatagacgc ggctataagt gtaatgggca
     5221 ttcctcggaa tgaaggggga acatgagcct cgttgatttt gcttctaaca atcgagaaca
     5281 taaaaatcgc gagcaaaaag ccaagtcccg cgccaagcgc gctgactatg ctctgcggga
     5341 atgtgtaagc ctcctgagca ttgatgatga caatgcccaa aacggcgcag ttggtggtga
     5401 taaggggaag atatattcca aaggccgaat aaagagcctt gatatacctc tttataaaga
     5461 cctcaagaat ctgaacaagt gaagctatta tcagtataaa aacggtggtc tgaagatatt
     5521 ccagtcccat gttgtttaaa agaacattaa agggatgcga gattatcgcg gcaagggtca
     5581 taactccagt aaccgcaagt cccatgccca ccgcggtatt agtctgcctc gaaactccaa
     5641 ggaaggggca gatgcctaaa aagcgggaga gaacaaaatt ctgtgtcaat atgcccataa
     5701 cgagcagagc tatataggtt ttcattattt cgcctcctta tcgcaggctt tgcaggagcc
     5761 gcagccgagt tccaccgagg gcttgccctt tttgtgattt atgcggttta ccagagccat
     5821 taagaagccg aaaaccataa agccgcctgc gggaagaatg aatatcttta tcggctcaag
     5881 ggctgtgtat tgcgaaaccg cggacgctac atcaacgccg ttaaaagagc tgccgctcaa
     5941 gaagggcaaa aggctttgaa tgccgctgag tattgtgccg ccaccgacaa attcccttat
     6001 aaggcttatg gaaaagagag cggcgaggaa gccgatgccc attccaagtc cgtcaagtat
     6061 ggacagcgca acggagtttt tggaggcaaa gctctcagcc ctgccgagta ttatgcagtt
     6121 aacggtgata aggggcaggt aaactcccag agcgtcgtaa atgacgggca ggaatgcctt
     6181 aaccagcatc tgcacaactg taaccattgt ggagataaca actatataag cgggaatcct
     6241 gacctttgag ggtattacct ttttaagcgc ggaaataatg gtgttcgtca ttacaagaac
     6301 cattatcgtg gcgattccca tgccgattgc gcttaccatt gtgttggaaa ccgcaagggc
     6361 ggggcaggtt cccagaacca gccttaaaac agggttttct ttaacaaatc cctttgaaaa
     6421 ggtctccaaa gggctgacct tagtttttgc catatcagtt agcccccttt tctatcagag
     6481 cgtttacttc aagggcggta tttacgcagg aggatacagc gcctgagctg accgtagctc
     6541 ccgaaatcgc gtctataagt ccgacctgac cgagacttgc gcccgtattt cctgccttag
     6601 aaaggataag ctctctgctt atgcccttga actgatttaa aaagctctcg ctgtttacct
     6661 ttgagccaag gccgggggtt tcgtgcagtt cgagtatttt aagccctgtg actttgccct
     6721 cggcgtcaat gcccgtaacc agcctgagta tgccgccgta gccgtttgtg ctggttataa
     6781 gaactctgcc tattctgttt ccgtctttgt caaaggcatc gtagtaagga tagggctcgc
     6841 ccctgcctgt gctgtattcc ttctgctcgg aaaagcggtc tgactctatt accgaggaga
     6901 gcatgttcaa aatctgctcc ttctcagcct tctctatata ctcctttgta aagccgttcg
     6961 taaatgccag aagaccggcg caaacaaggc atatcataag aagggacaag gtgggcagca
     7021 gaatgccttt aagcttttgc atgagctttg ccccctttct ttgtttgtcc aaagggtctc
     7081 ggtcttgtga gcctgtctat aaagggtgag aatatattca ttataagtat tgcgtaagaa
     7141 acaccctcat gcaggctgcc gaacagcctt atgaagcagg ttataagtcc gcagcctatg
     7201 ccgaatatga tttttccctt tatgctcatg ggacttgtgg tgtagtcggt tgccatgaag
     7261 atggctccca ggagaagtcc gcccgagagt atctgacccg cgacgaaatt taaacttccc
     7321 ttgcccgcaa tcagcattac aaccgccaca gtgcccacga acgaaacggg gatggaaagc
     7381 cttattacgc ctctgtaaag aaggtatata aagccgagaa gcagagcaag cgcgctgatt
     7441 tcacctatgg ctccctgcat ttgtccgagg aacaaagagg tcagcgacgg gaagtctttg
     7501 gcggcagtaa ggaaagagtc ggggctgccg gacttgaacg cctcgtaaag ggtatataag
     7561 ggcgttgcgg tggttactat ctcggagctg cttttccact ggaaaggcat tacgaaattg
     7621 ttcattgcgg cggggaagga aagcagcatg aaaattcttg cggtaagggc ggggttagca
     7681 aagttcctgc cgagtccgcc gaaaagctgc tttacaatca caatggcgaa tacgctgccc
     7741 acaaccacca tccaaagagg gatagtgacg ggcatattaa gagctatcag cattcccgta
     7801 accgctgcgc tcaggtcgcc tatggtattg ggctttttca taaagcggtt gtaaagatac
     7861 tcagccaaaa cagagcttaa tactccagta aaagcaataa ggaacgctct gggaccttgt
     7921 atccatgcgc ccgcagccag agtggggata agggcgatta taacatcaag cattactccc
     7981 tttgtggtaa ggcttgattt tatatggggc gagactgtga gtttcagctc gctgagattg
     8041 tctttattgt tatcctgaac cgagtttgcg cttttatcct cagatatcgc aggattcttt
     8101 gcattttcca ttattgcctg ctcctttcgc tttcgctttc gctacaagcc gctttcctat
     8161 atccagacct tttacaaggg gaattcccgc agggcagatg taggcgcagc agctgcaaag
     8221 tatgcaagcc tgagcgttga gcgccacaag ctcattaatg tccggcttgc cgtaatcggt
     8281 agctgccttg tatatatcgg taggcacgat tctcatcggg caaacctccg cgcacttgcc
     8341 gcagcgtatg caggaggtct ccgtcttaac ccttgccatc ttttccgcaa aggcgagaat
     8401 gccgtttgtt gtcttggtga cgggatagtc cgtgctgtca acggcgtgac ccatcatggg
     8461 accgccgatt atcagcttgt acggcttggc ctcaaagccg cccacgaaat cgattatgtc
     8521 agaaatcagt gctcccaagg gggcgataat gttggcgggc tctttgattg cagagccggc
     8581 tacggtaatg cagcggctta caagcggtct gcctgtcaga aggtaattgc cgaacgctgc
     8641 cgctgtcgat gtattcatta ccatgcatga ggcgtcggag ggcagctttc cttcgggcag
     8701 actaattccg agaagagcct taatgagcat cttctcagcg cccgttgcgt acttgggctt
     8761 aaggcgcaca atcttaatta tgtctttata ctctgacttt tctatcctct caataagcgc
     8821 ctgctcatcg cccttttcct ttttatcttc ctcagcgata agcacccttt caaagcctat
     8881 gtgctttata agggcttcaa tgcaccttat aatgtcgtcg ccgaagtcta tgcatgcgcg
     8941 gtagtcaacg gtaatatagg gctcgcactc aattgagttg atgataagcg tctttgcctt
     9001 tgcctcataa acggacctga gtttaatgtg tgtcgggaag ccggcgccgc ccatgccggc
     9061 aagacccgcg ttgaatacgg cgtctataag gctctcagcg tctgtgacgg tcgggggagc
     9121 gattgtttca agccactcat cattaccgtc ggattctatt gtgacgcaga ccgccttttc
     9181 cttgggggcg atttttctta ccgaaatatc cgtaacagtg ccggaaacgg aagcgtgtat
     9241 ccttgccgtg gcatcgcccg cgtcgccgat aagctgaccc tttttgacct tgtcgccaac
     9301 cgctacaata gccgaagcgg gttttgtgca gtgctgaagc agcggaatta caaccctgct
     9361 gacggacaag ctcttgggaa cagaatcaaa ggtggtcttg ttgtctttaa caagcacgcc
     9421 gcctttaatc ctggaaacgg gctttaaaaa tatgtctttt gcagttttca ttaccgattc
     9481 tcccgtaagg atatattttt acacaatagt atttgtgata ttgcctgatt tatataaata
     9541 agcggttcag tgatgcgtat ttttcgattg cgggagcgga gagattaagc actattcccg
     9601 taatcagccc cgaaactacc gatgcgaaaa gcataaaggg cagataagcc gcaaaaaaat
     9661 cagtgcccgt tacatacacc gcggcggcaa gctgaaggat attgaaggcc gcggcagaaa
     9721 gcatggacac tccggcaagg ctgatatgtc cctttttgta aagcacaccg atattcagta
     9781 ttattgcgga aatcacaaac gcgcccgcgc cgccggcaaa gccgattaaa aacgcgaatg
     9841 ttcctctggt caaaagggcg aaaacgctct ttgcgatgaa aataaagaga gagcctttga
     9901 ctccaaggaa taaaatcgca agcaatatca ccacattgga catgccgagc tttgctcccg
     9961 gcgggaagaa ggggaactgg ggcacaaggt tttcaagata agaaaggcaa atggcaagag
    10021 cgcttagtat cccgtacagg gaaataagca aaggataatt ctttttgccc tttttgcctt
    10081 gtcctttcat aaaatccccc aaaatagtat aaagcatttt ttggttttgg caacaaaaaa
    10141 ataataaata gttaaaataa tattcaaaaa gcaatttgat taataaacca cagcgtcaaa
    10201 atccgcgtcc gtttccccgc cctctataat gagcacgagc gacaagggca ggcagacgct
    10261 tgcgtcagcc tgctttgaaa tatatcccct ctgtatgcac agcttgtcat ggcagtcgga
    10321 atatataaaa gctgctgagc ccttttcata ttttattaca gcctttgagc cgtcaaattc
    10381 ataagttttt tcaatcggct cttcaattct gtcaaggctt attctctcaa tctcctcgcc
    10441 cttgcgataa agaacggcaa acctttcgcc gccgcctttg cccgcggggg caagcacgaa
    10501 tgagagcaaa gatatgctta tcaaaaagag agtgattgca atatcgcctt tttttatgcc
    10561 cgctttgtgt aacttcaata gcctgtcttt catacaagag taaattccga attcttaatt
    10621 gtgatgctgt ctttaatgcc gtcggtggct ttaaccgtct tgtcggggaa aacgaaaacc
    10681 gcctccgcgg agtattcctt taaaacgggg aggctctttt catagcccaa aacgaagcat
    10741 attgttgaga gggcgtcgct taaaagcccg ctgtcggaga acaccgtaac gctgacaaga
    10801 ccgctgtccg acggatagcc cgtaacgggt gagaaaatat ggtggtatct tacgccgtct
    10861 ttttcaaagt atttttcgta gttgcccgaa gttgaaacgc tggcggggcc cgttttaacg
    10921 cttgcgaagg tttcgctgac aggaccgaat gggtcgcgga tgccgatgct ccactgaacg
    10981 ccttcatttt tcgaaaacct tgagaaaacc ccatttgcgg gctttaagcc cacaaaggta
    11041 acgctgccgc cgacgctgat gctggcggcc ttgacgccca gctttttagc cgtttcagcc
    11101 gctatgtcgc aggcggcgcc cttgcctatg gctccgaggt ccacaagctg accctcgccg
    11161 attttagcct tgtttccgtt taatattatt ttcgataaat ccgctgtttc aagggcggtt
    11221 ttaatttctt cctcagccgg cacccgggga ttgtccgtat caatgcccca gagctttacg
    11281 agtttgccca gagcgacatt caaagccgag tcgctctttg cgcaaatctc aagagaagtc
    11341 ttaagtatct cagcggtttt ctgagaaatc tcaagctcag cgcccgcgtt ttcattgagc
    11401 ctaaaaatat ccgagccttc aacagtcggc gaaaggcact tttcgtcaag ctcttttatt
    11461 gcgcttatca tggcgtcggc gttatcctcc gtgccgccgt agagggtgat gaaaactgcc
    11521 gcgcccatgg cggaggagct tttgtttacg ctctgagcgc cgcccttaat gtcgcctgca
    11581 attaacatca gcaaaagaac aaccgccaga aaagcgaagg ctgaaagatt tttgtttctt
    11641 ttattgctgc tatttttcat agctgaatct cctaacagtg gaaagtgcgt ctttggcttt
    11701 gaccgttctg tcggctattt catccagcag agcgcggtca aagtatcccg ttaagtgatt
    11761 tctctgagcc gaagccgaga gaatggacac cgcggctctg gcgattccga gcctttttac
    11821 tataaagtcg gacttaatat caatcttttg aatgtcggaa aaattaataa gcaccgtttt
    11881 cctgaggagt attcccgaca ctattttcac gagtctttcg tcaaaggcaa tggcctttgt
    11941 tctgtaggac atatatgcga gaataaaaga aaaaattgca atggctataa aggaaatcca
    12001 ggttttttga attgggacaa agattaaagg cgccgaaacg ccgcttgaga aaaccgcggc
    12061 aatcatatat ggcaccagtg actttttgct tgcgggaata agctgtgagg atgtcatata
    12121 atcgggggcg caaagcttta aaaccctttc ggcctcgctc ttggtcgtaa gcaaagacag
    12181 cacgggcgtt ttctcctgct gagtggtaag acctataacg gcggcctcta tggagtaaag
    12241 cccgaatatc cttgcgagaa ggctctgctt tattatcacg ccgttaatct tgtccgcccc
    12301 gaaggcaatt gacgatgtct ttataagacc cttttttata taaatattcc cgccgtcctt
    12361 cattaaagta aaattccagt ttttgaaaac ggattttact actgaaaaca cggcgccaat
    12421 aagcagcagc cataaaagaa atatcctttg gtcggtgccg cccgcagagt atcttccatc
    12481 ctccaaaaaa acggggagga aggtaagaag ggcaagcaag ccctgagcaa agggaatact
    12541 caaaagctga tgcctgataa cctctgaaaa tccaaacttg attatcgtat cagcccttga
    12601 ttcctcaaag gcagagatga gcggcactgc ggtctgagta ttttcttcac ccgcttttgc
    12661 ctttataagc tcctctttaa aaagctcggc aagagggagt ttcagcacaa aagtgaagtc
    12721 ggtctgctcc gccgttgttg atgagttgat gtcaaccttt acttttgctg tgccgattag
    12781 cctttctatg aggcttctct caatgtttac cgttgaaata ttcgacgcgg gcagctttga
    12841 caccttttta aaaagggtct ttttatcaac caccaaaaca gagtcccgga tgctgaaaaa
    12901 agtctttctc caaacataaa agctgatgac gattacgatt acgcagatta aaaaaagtcc
    12961 cgcgccggaa agcagtacgc taaagtcgcc tgatttaagg gtatagaaaa atccccgcca
    13021 gtaggttaca gaaaaaatcc tgcttttgcc ctcaaggaac tgggaaaatc cctcggacaa
    13081 aagcataacc gctataaagc cccaaaaggc ggcgagcttt tcaaatataa cagaggggtg
    13141 atttcttata ggctccttaa acatcgcctt cacccgtgcc ttcgcctttt tgggccgcag
    13201 cgagcctttc ctttatcatt ttgttcagtt tctccgcgac agcgtcggct ctttcctttt
    13261 caagaaagcg gaaaaccgcc cttgagccgg cggtggtcac aatcaccttt gccacgccga
    13321 accttgcgtc aatgggaccg aaggcgattt caacctgatg aatcctgtca acgggcacga
    13381 ttgtgtgaga aacgaaaaaa actccctcta ttatgtcgat tctgtcgtca gaaatcctgt
    13441 atttatatcg cctgtggcga aactcggtta ttttgaagaa ataaaccaag caaagaatca
    13501 atatgactaa aatcatcaat actgaaaata ctggtcttat gggcagctta aaaagcagaa
    13561 gcgagagagc caataccgca aggcactgaa tgcccgctgt aactctcagc actccgagag
    13621 cctgctttgt tagacccata aattctccgc tttgtttgtt gtccataaaa cctccgtaaa
    13681 ttgtgccgac aaaaacacaa aaaattttac cataaatatt aaaccacaca gcttgggcat
    13741 tgtaaagcat tcgggcggat ttaaaagtgt caaaatatta acgagctcaa gccttcccac
    13801 cgttttaagg cttattaaag caaagcctat ttaaatgcac atgcggataa atttaatgac
    13861 tttacgcctt taatactaaa gtgccgattt aatctttata ttttaattgt gatatgctac
    13921 aattaacaaa aatgatttaa gcccgaattt aagggaggga gccaaatgtc gtctgtaagc
    13981 gaaaaaatgc ccgatggcaa gctctatata tcaaacgatg ttatagcaac catagcctac
    14041 aacgccgcaa aggaggttga gggctttgcg gggctttcaa agctcagccc ttcttcaaaa
    14101 tccctcttta aggcaaacaa gcactccatt gagataaaat cgggacagac gggcatagaa
    14161 atcagcatcg ccgtcgacct taaaagcgac gcgtcggttc agaataccgt caaactcata
    14221 cagagcaaca taaaaaacgc ggttcagaac atgaccggca atgtggttaa aagggtcaat
    14281 gtaaccgtaa acgatattgt atttgaagat taaagaaacc caaacggagg taaggatggg
    14341 acgcagagaa gaaagagaac aggcgtttaa tatagtattt tcttccctat ttcagccgga
    14401 ggaaccctta gaggatatag tcaaggctga aaccgaggcg gaggcttatg ttccgtcgga
    14461 ttattcgcgg aggctcatta agctcataag cgagaactta gaggaaatcg accgaatcat
    14521 tgaaaagagg acggtcaggt ggaaaaagga aaggctgtct aaggtggctc tttctgtact
    14581 gcggatttcc ctttgcgaga tactgtattt cgacgaaatt cccgtcagcg tcagcgtaaa
    14641 cgaggcggtg gagctggcga aaaaatactc aattcccgag gacgccgctt tcgttaacgg
    14701 acttttggga agctatataa gggaaagaga aggtcaaaac gccgaaaccg aggcaaagga
    14761 aaccgaggag cagtcagtaa aggaagaaat cgaggctctc gctgccgagt ccgaggcgga
    14821 aaagcaagct gagatggaag tcacaagcta aaactatgga catttatctc ggaatagaca
    14881 caagcaatta caaaacctcc gtggctgttt acgagcccga gtcggactcg attatagccg
    14941 acgaaagaag gtttttgact gtaaaagagg gtgaactggg tcttagacaa agcgaggccc
    15001 tttttcagca tgtaaaggag cttcccgctc ttatagaaaa agccttttcg gggctcaaaa
    15061 actttacgct gagagcctcg gccgcgagcg tgagccctac aaaccgggaa ggctcatata
    15121 tgccctgctt tcttgcgggg gagtcagcgg caagggcaat cgccgccgca aactcggtgc
    15181 ctttttacag aacgaatcat caggcggggc atgtcatggc ggctcttcac tcggcaaaag
    15241 cgttaagttt gctggacgaa ggctttgtgg cagttcatct gtcgggcggc acaacggatg
    15301 tgctcgaagt aaaaaaagac cccgaaagca ttatagaaat aaaacctctc tcggcctccc
    15361 ttgatttaaa ggcggggcag gtaatcgaca gggtgggcaa aatgctgggc atccccttcc
    15421 ctgcgggtga agaactcgag aaacttgccc ttaaatccga taaagagttt aaggtaaaac
    15481 cctattttaa ggacggagaa gccagctttt ccgggcttca gaatcagtgc gagcagaaac
    15541 tgaaaaaagg cgagagcaaa gaggatatag ctaaatactg catttcgtat gtgaccgagg
    15601 caataatact tatggctcag tccgcccttc aaagcacagg catgaagaag gcccttttct
    15661 cgggcggggt tacggcgaac tcatatctca gaaaaaaggc tttagagcgc cttgactgcg
    15721 ttttcggctc tcccgagatg tcgggggaca acgccgctgg aattgcggtg ctgtgctgcc
    15781 ttcttcacaa ccctcaaaag tagggggaat ttcatggctc aggaatatgt attaagcgta
    15841 tcgcagctga atttctatat aaagtccata atcgacgctg acagaaagct atatgacctt
    15901 tatgtaagcg gcgagatttc gaatttcacc cgccatttca gaacgggtca tctttacttt
    15961 acccttaaag acaaatcgtc cgccataaag gtggtaatgt tcagcaaagc cgccgacaga
    16021 cttcgcttta cccccgagga cggtatgcag gtcatagtcc gcggtcgagt ttcggttttt
    16081 gagcgtgacg gaacatatca gctttacgcc gaaagcattg aggctcagag cgtaggcgag
    16141 cttgccgctg ctctggaaag attgaaacaa aaacttaaaa gcgaaggtct ctttgatgag
    16201 agccgaaagc agcccattcc ccgtttcccc aaaagggtgg gagtggtaac ctccgcaagc
    16261 tctgcggcta ttcaggacat tctcagcatt cttgaaaggc gctggcccct ttccgaagtg
    16321 gttttctgcc ccgtcagcgt ggaggggctt aaagcggcgg aggaaatctc aaacgcgatt
    16381 aaggaattta acgacaaaaa agcggcggat gtgcttatag tcggcagggg cggcggctcg
    16441 gtggaggagc tttggacctt caacgacgag agaatcgcga gggcggtcgc agcaagccgc
    16501 atccccgtaa tctccgccgt gggtcacgaa agcgactata ctctcatcga ctttgtcgcc
    16561 gatttaagag cccccacgcc ctccgcagcg gcggagctgg cagtgcccga aagagcggag
    16621 ctgcttcaca gaatccgact ccttgagcaa aggcttaaaa acagcgtgga cgaaataata
    16681 aacacaaaaa cctacagact caggctcctt gaaaacaagc tcagctcaag agagccgacg
    16741 gcaatgattg aaaacaggat tcagcttatc gacaatctca ttcaaagact caataaaact
    16801 gcggacatat acttatccaa taagcaaaac agacttataa agaacatatc catgcttgac
    16861 gcgctgtcgc cgctgaaaaa tcttcaaaga ggctacgcaa tcgcaaaatc cgagaagggc
    16921 aaaatcataa aaagcgtatc tgctgtaaaa gtcggggaca aaatagatgt caatttgact
    16981 gacggagcat taaagtgtaa tatattagaa atgaaagagt tgtgataaaa tgcccgaatt
    17041 ttcctacgaa aagtcaataa aaaggcttga ggaaatcatc tcaaggcttg agcagggcga
    17101 cattgagctt gaggaagcgg taaacctctt tgacgaggga acaaagctcg ccgtttcctg
    17161 ccgcaagagc cttgaggagg caaagcagaa aatcaccctg cttaaaaacg ccggaagcgc
    17221 agagtctaat tagataggac agaattatgg ttaaagaagt actgaacgaa aacctgaaac
    17281 tgataaatcc ctttttaaaa gagaggatat ccggggcaaa tctttctacc gccgccgaaa
    17341 atctggttct tgacgcggcg gcgtactccc tttcctcagg cggcaaaagg gtgcgccccg
    17401 ttttgacctt ggagttctgc aaactctgcg ggggagacaa aaaagacgcc cttcactttg
    17461 ccgcggctgt tgagtttatt cacacttatt ctctgattca cgacgacctg ccctgcatgg
    17521 acgacgacga tgtaaggcga ggacagccgt cctgccatgt aaagttcggc gaggcggtag
    17581 ccctgcttgc gggcgacgct cttttgaccc ttgcctttga aacccttgcc gacgccccct
    17641 taaagtgcga gcaaatcgta aaggctgtta aggcgctttc atctcttgcc ggcgtcgagg
    17701 gcatgatagg cggtcagagc atagacattg acagcgagaa caaaaagctg actgtagaac
    17761 agcttgaaaa aatggacctt atgaaaacag gggcgcttat taaagccgcc tgcgttttgg
    17821 gctgcatagc cgcggacgca agcgaggaag aaatccagtc ggcaggcacc tttgcggaaa
    17881 gccttgggct tgcctttcag attatcgacg atattcttga cggagacgaa cagggcgagg
    17941 atgaaaagct gaataaagca acctatgttt ctgttcttgg tatggaagga gcaagagccc
    18001 ttgccgagga gcacacaaga aatgccctgt cggcgctcgc tccctttggc gagagagctc
    18061 agtttctgag ggagtttgcg ataacgctcc ttgagagaag cgtataagac tgtatgcgct
    18121 ttgttgtcag gtgaattaag atggaagaat ttaagttatt aaaaaacatc agcggacctg
    18181 cggatgtaaa gaagctcaaa aaggaagagc tgccgcagct cgcggatgaa ataagacagg
    18241 tgctgataaa caccgtttca aaaaacggcg ggcaccttgc ctcaaatttg ggcgtggtgg
    18301 agctttcaat cgcccttcat agggtatttt gctccccata tgacaaaatt gtctgggatg
    18361 tgggtcatca gtgctatgtt cacaagctct taaccggccg ctttgacagg ttcgacacaa
    18421 tcagaaccga gggcggcctt tcgggctttt gcagacccga cgagagcgag cacgatacct
    18481 tctactccgg tcacagcagc acatccattt ccgccgccta cggaatagcc gcggcgaata
    18541 aactaaaggg caacaaaaac tacaccatag cggtaatagg ggacggctca tttacgggcg
    18601 gtcttgtgta tgaggccctt aacaacgcgg gacgctcaaa aacaaggctt atagtcattc
    18661 ttaacgacaa tgatatgtca atttcaagga atgtgggagc tctcgcaagg tactttgccg
    18721 ccattaaggc aagacccggc tacttcagac tcaaggcaaa gaccgagcag cttattaata
    18781 agataccctt tgtaggcaaa aagctgtcca tcaccctttt cagaatcaag acctatatta
    18841 aaaacatgat atacaagagc accattttcg aagatttcgg ctttaggtac atgggcccaa
    18901 tcaacggaca caacataaac cagctcatcg acgctcttga aggcgcgaag gcggcaaatc
    18961 acccagttgt gctccatatc aataccgtaa agggaaaggg ctacgaccat gctgaaaaat
    19021 cccccgaggt gttccacggc atctcccagt ttgacataaa tacgggcgag ccgctttccg
    19081 ccggaacgaa tttctccgcc gagttcggca gattcctgat tgatgttgcc gagaaggaca
    19141 gaagaatctg cgccataacc gccgccatgt ccgtgggaac gggtcttgac ggctttgccg
    19201 cccgtttccc cgacaggttc tttgatgtgg gcatagccga ggctcatgcg gtcacctttg
    19261 cgtcaggtct tgccaaggcg ggaatggtgc ccgtattcgc ggtttactcc agcttccttc
    19321 aaagggctta tgaccagctt atccacgacg gagctctcca aaggcaaaag atgattatag
    19381 ccgttgacag agcaggcttt gtgggcgagg acggagaaac ccatcaggga attttcgatg
    19441 tcgcgttcct aaacagcatt ccgaatgtaa ccgtctattc cccgtcaacc tatgcggaga
    19501 tgcgccgctg ctttattaac gccttttatc atcaggacgg tcttgcggcg gtcaggtacg
    19561 caaggggaac cgagtgcatt ctgccggacg attttgagcc cacattctca gattacgagg
    19621 tctacggcga caaagaggcg tatacggtta tagtcaccta cggaaggctt ttcggctttg
    19681 ccgccgccgc tgtcagaagg cttaaagagc agggtatccc cgtcatgact ctgaagctta
    19741 caaagataaa gcccatagac aaagacgccg ttaccattct cctcggcaaa aagaagattt
    19801 tcttctttga ggaaggcgta aaatcgggct cagtgggcga aaggcttgct ttgattctca
    19861 aggagcaggg ctatgaaggc gagtacaccc tcaccgccgt aaacgacagc tttgtgcccc
    19921 acgcccccgt aaagagcctt cttaagagat acaagctgga caccgacggc atgtgcgaga
    19981 ttataaccca atcctttcaa aaacccgcgg cgataggctt tgagccgtaa agcggaggca
    20041 gtatgtcgga acttcaaaga cttgataccg aaatagtaaa aagagggctt tacgacagcc
    20101 gtcaaaaggc agccgccgcc atagcggagg gcagggtaaa ggtaaacgga aagacgataa
    20161 ccaaaagctc ctataaagtc agccccgccg acagcataga aatagaaaag ggcgtttctg
    20221 acgagtatgt ttcaaggggc gctcttaaac tcaaaaaagc cctcgatgtt tttaatataa
    20281 gcgtgcgggg gcttgactgc cttgacattg gctccagcac gggcggtttt acagactgcc
    20341 tattaaagaa cggagccgca agagtcattg ccgtggatgt gggcacaaac cagctccacg
    20401 atacactgcg gcaggacaaa cgagtttttg tgtatgaaaa tacagacata agggactttg
    20461 caaaggaaac gctgcccttt aatgtgcggt ttgtttcctg cgatgtttct tttatttctc
    20521 ttgtattaat tttgccttca gtatataatc ttcttgaaga caaagggcag gccgttctgc
    20581 ttataaagcc ccagtttgaa gcgggcaggg agaacatagg caaaaagggc attgtgaaag
    20641 atcggaaaat tcatacagaa attattcata aaatttatag aagcgcaaag gatttaggct
    20701 tttttgtctg cggtcttgat ttttccccaa tcaaaggcgg cgacggaaat attgagtacc
    20761 ttatacacct taaaaaaggc gacaatgaag gggaaataac agactttgac gcccttatag
    20821 aaagaacggt aaactttgct caaaatttaa actgatacgg cggtgaataa aatgctggct
    20881 gccattatgc caaaccttac aagaaaaaat gcctttgatg tcaccgttgc agcctgcaaa
    20941 aaactcgact ccgcaggaat tgcctacgct tttcccgagg agtacagagg ggactttccc
    21001 gttctgacaa acgcctcttt ttataaggag gataggcttt ttaaggactg cgatttgatt
    21061 attgccgtcg gcggcgacgg ctccgtaatg cactctgcaa agcacgcggc cttaaactca
    21121 aagccccttt tcggcattaa cgcgggaaac cttgccttta tggcaagcct tgagagcgat
    21181 gaactggact accttgaaag gctaaagaca ggcgacttta gaatcagccg cagaatgctc
    21241 cttgaatacg agctttacga tgaggaaaag ctcataaaaa aagactactg cattaacgac
    21301 atagttttcg cgaggggcag tcagataaag ctctcccata tcaatgtgga gtgcaacggc
    21361 agagaaatcg gcaaatactc ggcagacggc attatcctct caacccccaa cggctccacc
    21421 gcctattctc tcgcggcggg cggtccagtg gttgaccccg atatccagtg cataatactt
    21481 acccccgtat gccctcactc tctttttgcc aggtccgtaa tcttctgccg cgacagcgtt
    21541 ttcaccattt ctcccgccga ggaagaaaac tcccccttag taatttcctg cgacggcgag
    21601 gaggcaatgc ccctgaataa aagcggcaaa tgcgtcataa aatgcgccga tattgatgtg
    21661 ggctttataa tcttcaaaga ggatactttt acggatgttt tatacaacaa actcgcaacc
    21721 aacagctaaa ctgcggtctt aatcccttaa cacaacgaaa taggaaggaa agagctttcg
    21781 tatgaaaaaa gcaagacatg agcttatctt aaagctgatt gaagaaaacg acatcagcac
    21841 tcaggaagag cttcttgaaa agctgaagga aagaggcatc aaggtaacac aggcaaccgt
    21901 ttcaagggac atcaaggaat taaggctcat aaagcagccc tccgcaagag gcgagtatcg
    21961 ctatacctgc gcaaggaaca ccaccgatga tgaggacgcc cgtttccata cggttttcgc
    22021 aagctccgtc atctccgtgg actacgccca aaacatctgc gtcttaaaat gccatacggg
    22081 cactgctcag gctgcatgcg cggtgtttga cgagatgaag tggcacgata cggtgggcac
    22141 ccttgcgggc gacgacacca tattcctcct ttgcagaacc gaggctgccg caaagaacat
    22201 ctgcgaaacc attgcaaagc agcttgatat ataattaatt ctttcttaaa ccgaggcggt
    22261 ggtttgttgc tagccacttt aaatattgag aatattgccg tcatagaaaa agctaacata
    22321 gatttttccg acggctttat ttgcctaacc ggcgaaaccg gcgcgggcaa atctattatt
    22381 atcgactcaa ttaacgccgt aatgggcgaa aagacatcca gagagctgat aagaaccggg
    22441 gcggaaaccg caatggtcag cgccctgttt
//
LOCUS       DAONVQ010000015        21314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_1781_length_21314_cov_4.590620, whole genome shotgun sequence.
ACCESSION   DAONVQ010000015 DAONVQ010000000
VERSION     DAONVQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 21314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21314
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1781_length_21314_cov_4.590620"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1342)
                     /locus_tag="PLD98_02050"
     CDS             complement(83..1342)
                     /locus_tag="PLD98_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA05066.1"
                     /translation="MINYDIAVIGGGPAGLAAALKAKELGIEKIIILERAETLGGILE
                     QCIHTGFGLHYFKEELSGPEYAGRFIEELKKTDIDVRTNTMVVDISSDKVVTAVGPNE
                     GLLKIQAKAIILAMGCRERPRGAISIPGNRPAGVMTAGTAQKYVNIHGYMPGKKIVIL
                     GSGDIGLIMARRMTLEGAEVKMVCEILPYSGGLARNIVQCLDDYGIPLKLSTTVVEIH
                     GRERVEGVTIAKVDEKMRPIKGTEEYVECDTLLLSVGLIPENELSKSAGVDINCITGG
                     PDVDEMRETSIRGIFACGNVLQVHDLVDYVTLEAQLAAEGAAEYIKKGEEEKKYAYTV
                     NQNGVRYIVPQKININADRDVDLYFRVCQIYKNAKVVVKIDGETVISRKKPKLAPGEM
                     ETVKLKKDMIAKLKEDSLVEISVEESL"
     gene            complement(1352..2785)
                     /locus_tag="PLD98_02055"
     CDS             complement(1352..2785)
                     /locus_tag="PLD98_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQA05067.1"
                     /translation="MYDVAVIGAGIIGALTARELAKYKLSAVLIEKCNDVAMGTTKAN
                     SAIVHAGFDAVPGTKKAIFNVLGAELMPKVTSELSVPYRNNGSLVVAFSEEELPELQN
                     LLERGIKNGVKDLKILSKEELLELEPNISPEAVAALHAPTAGIVCPYEFAIAATENAV
                     TNGVEFIRNCEVKDVEVHDDYTTLITNTGDIKAKVVVNAAGVFSDDIARMFGDDTINI
                     VARKGEYYLFDKAVGHVVFHTVFQCPTPMGKGVLVTPTVDGNLLIGPTAVDIEDKNDT
                     STTYEGLMEVLNKAHKSVPTIINTRDAITQFSGLRAHAKSGDFIIGFSEKAPSLYNLA
                     GIESPGLSAAPAIAVHAAQEVANKLGLTELKEDYNPIRPQPIRFRYLDDEEMAELIKK
                     DPRYGRIICRCETITEGEIVEAIHAPAGARDIDGVKRRTRAGMGRCQGGFCSSKVLEI
                     LARELNIPYEEVTKEGGNSRILYGRTK"
     gene            complement(3176..4207)
                     /locus_tag="PLD98_02060"
     CDS             complement(3176..4207)
                     /locus_tag="PLD98_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05068.1"
                     /translation="MKKLISVLLIAVLLIASVPFVVTADTIDTDIDVDTTISGDDIIP
                     ADKIITLTKTLIIAPGASLTVYGQLKIGFGGSIIVDGTLNVYGKVTNVAGADPVVRGQ
                     GVIMNAVTLPGSKPGLYTVYKMTYDPILKIYHEEEVATSTTYLVESGGEFAFNVKMLQ
                     PQHDPVTFPVKANGMTVYNEAGYFKVTGIVEPINFTFGDPQIKIFKVRLPKGKGYIVA
                     ARGQAGVPDSEVTDVVYIKYGEDLEFTLRINENYDKSSNIQVYVNGNLVVPDAYGYYR
                     VPALGTDGATSYQIDVFGVVSNELSDTLTAVIQLFKNIFEAFKGIIDALREIFAELGG
                     IGGGLLPTP"
     gene            complement(4339..5406)
                     /locus_tag="PLD98_02065"
     CDS             complement(4339..5406)
                     /locus_tag="PLD98_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HQA05069.1"
                     /translation="MENITSEISRKPIFFERNRVFRVYLGGKLFNDFFGDEPTDGNYP
                     EEWIASAVKALNSNSTDPNEGLSKVQGTDIYLDELIEKERENILGGHDSLGILVKMLD
                     SAVRLPIQAHPDKPFSRKHFNSEYGKAEAWIVFATRENAKIYFGFKDKITKEEFVKAI
                     ERSETEKDAMEELLNEIPAKAGDVYFIPAKQVHAIGYGCMILEIQEPTDFTIQPEAWC
                     ADYKLNDYEKYLGLNPDDALECFDFSVYGDAAVALGKKAPKIIKEDGGVVSEQLIGRE
                     DTPCFAVKRHTVTNGTFRELSAPAVYVVTDGEGEIKGEDYSKPIKKGDYFLLPKAAEG
                     RFEIASNSGIQIVECIPPFNV"
     gene            complement(5408..6775)
                     /locus_tag="PLD98_02070"
     CDS             complement(5408..6775)
                     /locus_tag="PLD98_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQA05070.1"
                     /translation="MKLLGLDIGTTSICVNVIDSETGQVICTSNVPNTSFIHTDRAYE
                     KIQDPARILSCATHAVEAMIEKHSPIDAIGISGQMHGILYLDAEGNAVSPLYIWQDGR
                     GDQEYKDGKSYAEYMSEVTGCDLATGFGTVTHFFNMKNSLIPEKAVKLSTIHDYVGMK
                     LTGGKEPVTHPSDAASLGLYNLTEDKFDEAAMEKLGIDPSFFPEVKKGFEIIGKTKHG
                     IPVAVAIGDNQASFMGSVKDSESSILVNVGTGGQVSMVTDKPLYNQYIETRPWTDGKY
                     LLVGPSLCGGRAYAILERFFRSVVQLTDAEVFTMYPIMDKLSEGYKTLTDKLEVSTLF
                     CGERGNPSKRGSISNISADNFTPQHLVVGVLEGTVNELYEKYTYMSENIATPPSTLVG
                     SGNGIRKSKVTRSMFAGKFGMEVKVPAHKEEAAYGAALFAGVAAGVFKSLKEAQSIIK
                     YEKED"
     gene            complement(6789..9161)
                     /locus_tag="PLD98_02075"
     CDS             complement(6789..9161)
                     /locus_tag="PLD98_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HQA05071.1"
                     /translation="MSIQTVYTADKITALAKELFKEERSKKRLDGWFRIKEIQKEADE
                     KYKGEHIEIKAAKTLRDIAEKLPIDISDYAVFAGTQDDAFARSYALINPSFEVESFSG
                     YCDPTAVFNDIEPSEEISAEHIEEMREYNKNTDYVRQLSEAYSKAEKYTSEVVYFFEQ
                     VTGHLIPDMRPALAHGIESLIADIEKRISAETDSERKANLEAMKIALEAAVILAGRYS
                     ALAKEKAEKASGESKERFELMAKTLAKVPAKGADNLYEAIQSFIILWQVMCIEQSPNP
                     FAFSVGNADRIFEPYRAKENLSRELASALFKHFLVFFNVGDRSWAISQNIILGGRDVD
                     GNDLSNESTFALFDAYYDMNLPQPILSVKLHKNTPDSIYEAMGKFFFTPGMLTPSLFN
                     DDAMFQVLERAGRDKEDLPDYSIAGCQEPLIMGKDNGNTTNSWLNLGKILELTISDGV
                     STVTGERIFEPHGPKDPVERLKNIRELFYQNVEKYTKIMADSANDASRALSNLRVPFL
                     SVLMGCAERGYDMRDCDHQGTKYNGSGCLIHGVSVVADSFVAIDKLLEERPEDAERLI
                     EALRNNFEGDEELRRYLQSAPKFGNNEEIVDNEAADVANKVADIVASQKNYLGNPFRP
                     DFSTPSTHLLYGYWVGATPDGRKSRDMLNYGLDPLYGDAHQGLGFRTLSAFKLPYEKM
                     NGGYASHFGIDPKYFTSESYEGKGLDFKKRVIAPLFFNEAKEGVSPFYLYVNVTTAEI
                     LRKVLENPKKYAPSGVYIMRIHGTFVNFLDLSPAIQKDIITRLDPASTSM"
     gene            complement(9183..10052)
                     /locus_tag="PLD98_02080"
     CDS             complement(9183..10052)
                     /locus_tag="PLD98_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HQA05072.1"
                     /translation="MRITVFQKGFNYSQDGQGNRLVFHLQGCNMRCPWCANPEGIPVV
                     PPIMVNKEKLLDSACPYGAVKDKELDRSRCEKCPDRRCLQSGVDLGLYTAASDRSTDD
                     IIAEAVKCKPMYFDGGGVTFTGGEPTLQFDALKEVYKGLKANGIHTAIETNGTSPKLP
                     ELMPYIDQLIIDLKHYDSKKQKEVTGVGNENTKANIEQALKIGKSVLVRIPLVNGFNA
                     AREDALGFAGYFESINQDGLSVELLPYHEFGKDKWLKCGMEYTVNDGYIKPGTLKMFE
                     DIFKERGIKHIRT"
     gene            complement(10337..10552)
                     /locus_tag="PLD98_02085"
     CDS             complement(10337..10552)
                     /locus_tag="PLD98_02085"
                     /inference="COORDINATES: protein motif:HMM:NF022659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HQA05073.1"
                     /translation="MYIIDRFEESIAVIEDDDGKMIEVNRSLLPKNAKEGSVLIKNDD
                     GSFSVDEKMTAERRRKLKAMQDALFGN"
     gene            complement(10563..11504)
                     /locus_tag="PLD98_02090"
     CDS             complement(10563..11504)
                     /locus_tag="PLD98_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HQA05074.1"
                     /translation="MQNNRKLKAVLSAVALLLFLSILVPFVFMKNPNGDIPTTEDVIE
                     SAALVEETSVPVTDSEELTVTFIDVGQGDSTLIQCGDKLMLIDAGESGNEDKILNLIH
                     KSGFDKIDLLIATHPHSDHIGSMPAILKRIKVDTVIMPYISEASMPTTRIYEKSLQAL
                     SESGAKVERAVPGKEYSLNNLKITILAPFTDTAELNNLSVVLRADFKETSFLFTGDAE
                     KKAEQSILKNNMNVRAAVLKVGHHGSRSSTTEEFLSKVSPQYAVISCGLKNSYGHPSK
                     DTIGRLEKHNIKYYRTDYNGNITVITDGKTLEFKTER"
     gene            complement(11523..14066)
                     /locus_tag="PLD98_02095"
     CDS             complement(11523..14066)
                     /locus_tag="PLD98_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HQA05075.1"
                     /translation="MAETKKTSSAAGKGNAAKSGSGASSQKSGAKKKTRAAENVAPYD
                     EYTPIYKNEQFWAIILFAFALFLLMLSIFEGGPDTIWTKMREIRFGLLGFTAFLYPFA
                     LICISVMLTYNKKKTTIFTGSLSLFVIILFISSLLFITRTDPPFEVWNDAKEQYKLFA
                     DSGIKSGSGFFGALIGGLLISIADSKAPAIVISLLTIFVFLMLATGTSLMKLFSAVGK
                     PVKKVSDISAEKIVELKNRPKFDVDVDLGPPPSTESPFEDEPKEIPISGYPNVFDESY
                     KKEKAPAPIIDIPLEKEPEPEPEPQPPKAKSVFDAEKEASIKEREENIFALEDEIAPN
                     QDKPEKPYVLPPLNLLNPPSKSSASQSAAELKATGEKLINALKSFNVEASLLNIIPGP
                     SVTRYEVAPAPGVKISRFTGLAEDLALHLAAPAGVRIEAPIPNKSAIGIEIPNSGRAT
                     VSFRSIIDSENYRKNKSLLKVALGKDIAGVPICADLAKMPHLLIAGTTGSGKSVALNA
                     MIVSLLYNATPDEVRLLLIDPKQVEFSVYNGIPHLLVPVLSDARKAAGSLGWAVTEMI
                     DRYKTLNDNGVRDIKAYNALCEENKELKRMPQIVIVIDELSDLMAVAPSSVEDSIMRL
                     AQMARAAGIHLVVATQRPSVDVITGLIKANIPSRIALSVSSQVDSRTIIDSAGAEKLL
                     GLGDMLFCPVGAPKPIRIQGCYLSDAEIERVVDFVKKQGECRYDEDVASEIERQAAQE
                     KGKHSSSSDGETSLDEGTKDLIMKALEFVVSNPEKASITNLQRRLGLGFAKAGRIMDE
                     LEERGIVGPPEGSKPRKVLITKAQWLEMNARQSDDAPTESHREETADII"
     gene            complement(14103..14966)
                     /locus_tag="PLD98_02100"
     CDS             complement(14103..14966)
                     /locus_tag="PLD98_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQA05076.1"
                     /translation="MEILKAIFLGFVQGVTEFLPISSSGHLSLVQYFFGSTGEESLLM
                     TVLLHLGTLLAVFIVFYKTIWEIFLEFFKIIKDIFTGKFSFKDMSETRTMLFMFVLSC
                     LPLLLLLVPVSGDMRLMDKLAVFSTDKSILAEGVCFIFTALLLITGSYIAKNMISPKK
                     ISPKSALGIGFAQLFAAAFPGISRSGSTISTGLIFGLSREYMVKYSFILGIPAILAAN
                     AVELKDAVSQGSDINWIAAAAGVITAAAVGVGAIKALQYLLKKDMFKYFGYYCFAVGV
                     FAIIVSLIEVL"
     gene            complement(15032..15808)
                     /locus_tag="PLD98_02105"
     CDS             complement(15032..15808)
                     /locus_tag="PLD98_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HQA05077.1"
                     /translation="MTPQVKGDDDEANQTPEDTQDLIKETGSVTVSKQGRLIHCLTII
                     GQIEGHYTLPSQNKTTKYEHVIPQIVAVEESKDIDGLLIVLNTVGGDIEAGLAIAELI
                     AGMRKPAVSLVLGGGHSIGVPLAVAAKRSFITPSATMTIHPVRMNGLVLGVEQTFAYF
                     QSIQERIVKFVADNSNMPADRFRELMLASGELVMDVGTILKGEQAVNEGLIDSLGSLS
                     DALSCLYSMIEEFKKAEGESEENTAPESDGGEEKNQPNQP"
     gene            16134..17060
                     /locus_tag="PLD98_02110"
     CDS             16134..17060
                     /locus_tag="PLD98_02110"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQA05078.1"
                     /translation="MFPSSSGLGDIFARSYELSNISETKGVFQIVHSITEHSERYKEF
                     AVFLCSRGYAVFVHDHIGNGRSVLADEAKGYFGEQGGASTLIADTYELTRLISAKYAG
                     LPIFMYGQGFGSFIAREYCVAHKYDLKGAVFCGTGSASPTVSVGRAVAKILTEKNGSD
                     YRSDFLNNMLLGANNKRCKPSKSNFDWLTSSEEDLEKFLKDPLCCTEGLTVSAYADIL
                     SIVQRVNTKDWFKNIPKDLNLLLISGTEDPIGNYGKGVKEVFDRLQAAGHKNTLLKLY
                     EGCRNELIHECGKYSIFDDIADWADSVIENKA"
     gene            complement(17102..18421)
                     /locus_tag="PLD98_02115"
     CDS             complement(17102..18421)
                     /locus_tag="PLD98_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HQA05079.1"
                     /translation="MKLTFMGAGSTVFAKNVIGDVMLTESLRDAEIALYDIDAERLEE
                     SYIMCTALNRNINENRAKVKKYLGVENRKDALRGADFVVNAIQVGLYDPCTIIDFEVP
                     KKYGLRQTIGDTTGIGGIFRALRTIPVLKDFAEDMQEVCPDAWFLNYTNPMGMLTGYM
                     LRYTGVKTVGLCHSVQGCSRGILRDLGIEYKEPIKERIAGVNHMGWLLEIYDADGKDL
                     YPEIRKRAIEVLESGNKEHHDLVRYEYIRRFGYYCTESSEHNAEYNNFFIKDKYPELI
                     ERYNIPLDEYPRRCVNQIERWKNDKLKYLNDEVSHKKTHEYASGIMNSMMTDTPYKIG
                     GNVINDGLIPNLPKEACVEVACLVNKLGVQPCKQPPLPVQLAAMNMTMINVQLLTIEA
                     AVTQKKEHIYQAAMLDPHCSSELSIDDIVKLCDDLIEAHGSWLPKYR"
     gene            complement(18580..19128)
                     /locus_tag="PLD98_02120"
     CDS             complement(18580..19128)
                     /locus_tag="PLD98_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQA05080.1"
                     /translation="MSRERAEELLKEYNKEEFHLKHAYTVEAVMGYFAKELGYGEDEE
                     FWRIVGLLHDLDYELYPDRHCIKAQEILRENGASEELIHATASHGYMLTVDIEPEHIM
                     EKVLYAVDELTGLIGAVALMRPSKSVQDLELKSVKKKYKSPSFAAGCSREVIQRGADM
                     LGWELDELITKTILAMRTIPGA"
     gene            complement(19164..19532)
                     /locus_tag="PLD98_02125"
     CDS             complement(19164..19532)
                     /locus_tag="PLD98_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQA05081.1"
                     /translation="MKEVSTEKAPKAIGPYSQAIVASDMVFVSGQIPLVPDTGEIPEG
                     IAAQTRQALKNLAAVLEAAGSSVQKVVKTTCFLKDLSMFTDFNKEYENAFPHKPARAC
                     VEVSRLPKDVLVEIEAIALL"
     gene            complement(19569..20318)
                     /locus_tag="PLD98_02130"
     CDS             complement(19569..20318)
                     /locus_tag="PLD98_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HQA05082.1"
                     /translation="MNQFTRTELLIGPEGMEKLKNSRIAVFGIGGVGSFALEALVRTG
                     VGEVDIFDDDKVCLTNLNRQLFALRSTVGKYKVDVAKERMLDINPNVKVNAHRMFYLP
                     ENADEVDLSVYSYVIDAVDTVTAKLELCERAYKAGVPIISSMGAGNKLDPTKFEVADI
                     YETSVCPLARVMRRELNKRGVKSLKVVYSKEPPMTPRDPFEACRKNCVCPPGTERTCL
                     SRRQIPGSIAFVPSVVGLIIAGEVIKDLLAK"
     gene            complement(20405..>21314)
                     /locus_tag="PLD98_02135"
     CDS             complement(20405..>21314)
                     /locus_tag="PLD98_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQA05083.1"
                     /translation="TTFVSGDKPENFEAAIRPETRAIFVESIGNPNSTIVDIDAVAEI
                     AHKHEIPLIVDNTFSTPYLFRPIEHGADIVVHSATKFIGGHGTAIGGVIIDSGNFDWA
                     ASDKFPGLSKPNPSYHGVVFTEAVGRAAYIIKARTTLLRDTGAALSPFHSFLFLQGLE
                     TLSLRVERHVYNALKVVEYLRNHPLVEAVNHPSIETHPDHEIYKRYYPNGGGTIFTFE
                     IKGDAETAKKFIDSLELFSLLANVADVKSLVIHPASTTHSQMTEEELLESGIKPNTIR
                     FSIGTEHIDDILADIENAFNKVSGKA"
BASE COUNT         4860 a         5141 c         5303 g         6010 t
ORIGIN      
        1 gtgacggaga taagctcctt gccctcgtac tcaacatcaa gtatgcatcc gataggacaa
       61 gctatacata tcatttgctt tttcataggc tctcctcaac agatatctct acaaggctgt
      121 cttctttcag ctttgctatc atatctttct taagtttaac ggtttccatt tcgccggggg
      181 ctaatttggg tttctttctt gaaattacgg tttcgccgtc tattttaacg acaacttttg
      241 cgtttttgta gatttggcaa acacggaaat aaaggtctac atcacggtca gcgttaatgt
      301 ttatcttctg cgggacgatg taccttacgc cgttttgatt gacggtgtag gcgtactttt
      361 tctcttcctc gcccttcttt atatactctg ccgcgccctc tgcggcgagc tgagcctcaa
      421 gggttacata gtccacgagg tcgtgaacct gaaggacatt gccgcatgcg aaaatgcctc
      481 ttatggaggt ctccctcatc tcgtcaacat cgggaccgcc tgtgatgcag ttgatgtcca
      541 cgccggcgga cttggaaagc tcgttttcgg gaataagtcc gacggacaga agtaaggtat
      601 cgcactcaac atattcctcg gtgcccttaa tgggtctcat tttctcgtca accttggcta
      661 tggttacgcc ctcgacacgc tccctgccgt ggatttccac aacggtggtg ctgagtttaa
      721 gcggaatgcc gtagtcgtca agacactgga caatgtttct tgcaagaccg cccgaatagg
      781 gcagaatctc acaaaccatc ttaacctcag cgccttcaag ggtcatgcgg cgcgccataa
      841 tgagtccaat gtcgcccgag ccgagaatga caatcttttt gcccggcata tagccgtgga
      901 tattgacata cttctgggcg gtgcctgcgg tcattacgcc cgcgggacgg ttaccgggga
      961 tggatattgc tcctctgggt ctttcgcggc agcccatggc aaggataatt gcctttgcct
     1021 gtattttcaa aagtccctca ttgggtccga ctgcggttac aaccttatcg ctgctgatgt
     1081 cgacaaccat tgtgttggtt ctgacatcaa tgtcagtctt tttaagttcc tcgataaagc
     1141 ggcctgcgta ctcgggccct gaaagctcct ctttgaaata atggagaccg aagcctgtgt
     1201 gaatacactg ctccagaatt ccgcccaagg tttcggcgcg ctcaaggatg attatttttt
     1261 ctatgccgag ctcttttgcc ttcagcgcgg cggcaaggcc cgcgggacct ccgccgataa
     1321 cagctatatc gtaattaatc ataattcagc ctcatttcgt cctgccgtat aatattcttg
     1381 aatttccgcc ctctttggtg acttcctcat aggggatatt aagctccctt gcgaggattt
     1441 caagcacctt ggaggagcag aaaccgccct gacagcgccc catgcctgct ctggttcttc
     1501 tctttactcc gtctatatcc cttgcgcctg cgggggcgtg aattgcctct acgatttcgc
     1561 cctctgtaat ggtttcacag cggcaaatta tgcggccgta gcgagggtct ttctttataa
     1621 gctctgccat ttcctcatcg tccaagtacc tgaacctgat aggctgggga cgaatcgggt
     1681 tgtaatcttc tttcagttcg gtcagtccca gcttatttgc tacttcctga gcagcgtgaa
     1741 ctgcgattgc gggagcggcg ctgagtcccg gagattctat gcccgcaaga ttatagagtg
     1801 acggcgcttt ctcgctgaag cctattataa agtcgccgct ctttgcgtga gctctgagac
     1861 ccgagaactg agtgattgcg tcccttgtat ttatgattgt gggtacggat ttatgggctt
     1921 tattaagaac ctccataagt ccctcatagg tggtggaggt gtcgtttttg tcctcgatgt
     1981 caacggcggt gggacctata agcaggttgc cgtcaactgt gggtgttaca aggacgccct
     2041 tgcccatggg tgtggggcac tggaatacgg tatggaaaac cacatgaccg acagccttgt
     2101 caaagaggta gtactcgcct tttcttgcga ctatgtttat tgtgtcgtcg ccgaacattc
     2161 tggctatgtc gtcggagaat acgcccgcgg cgtttaccac taccttggct ttaatgtccc
     2221 cggtgtttgt aattaaggtg gtgtagtcgt cgtgtacctc tacatcctta acttcgcagt
     2281 tgcgaataaa ctctacgccg tttgtgacag cgttttcggt cgcggcaatc gcgaactcat
     2341 agggacaaac aatgcctgcc gtcggcgcgt gcaaagcggc aacggcttct ggtgaaatat
     2401 tgggctcaag ctccaaaagc tcctctttgg agaggatttt taaatcctta acgccgttct
     2461 taatgcctct ttcaagaagg ttctgaagtt cgggcagctc ctcctcagaa aaggctacga
     2521 ccaaagagcc gttgtttctg taaggaacgc tcaactctga agtaaccttg ggcataagct
     2581 ctgcgcccaa aacattgaaa atagcctttt ttgtgcccgg aacagcgtca aatccggcat
     2641 ggacaatcgc gctgtttgcc ttggttgtgc ccattgccac atcgttgcat ttttcaatta
     2701 agacagcgct gagcttgtat ttcgcaagct cccttgccgt caatgcacca attatacctg
     2761 ctcctataac tgctacatca taaatcataa aaaagctcct gacaagttca ttagttttgc
     2821 gttagtatta taaaccgctg tgagaataac ttcaagcggc gcgaattaag taagacttta
     2881 tttatatatt agcaccaaat attatcaaaa tcaacaaaaa caccgattaa acactacatt
     2941 ttgttttata ttcccaaaag gaaaaatggg gctcaatttt atttataaat gcaaaaaaaa
     3001 caccgcccac agcgccatcg cactgtgagc ggctgcggat gtaacgcaaa cgcaattttt
     3061 tttatcagtt catccgcaag tatagattat gaaattttta ttcctttata agagttaatt
     3121 taaggaaaaa acagggcgaa caatcgccct gcttatcatt ttaattaatg aagttttatg
     3181 gtgtgggaag gaggcctccg ccaataccgc ctaactctgc aaagatttcg cgcagagcgt
     3241 cgattatgcc tttaaatgcc tcgaaaatgt ttttaaagag ctgaattact gcggtaagag
     3301 tatctgacaa ttcgttggaa acaacgccga atacatctat ctgataagag gttgcgccat
     3361 cggttccaag tgcgggaact ctgtaatagc cgtatgcgtc gggaactaca aggttgccgt
     3421 ttacataaac ctgaatattg cttgatttgt catagttctc atttatacgg agggtgaact
     3481 caaggtcctc gccgtatttg atgtaaacaa catcggtaac ttcgctgtcg ggaacgcctg
     3541 cctgaccgcg tgccgcaacg atatagccct tacccttggg aagtcttacc ttgaaaatct
     3601 tgatttgcgg atctccgaaa gtgaagttga tgggctcaac aatgcctgtt accttgaagt
     3661 agcctgcctc gttgtaaacg gtcatgccgt ttgccttaac ggggaaggta accgggtcat
     3721 gctgaggctg aagcatcttt acattgaacg caaactctcc gcccgactca acaaggtatg
     3781 tagtgcttgt tgcgacctct tcctcatgat atatcttgag aatcgggtca tatgtcatct
     3841 tgtagacggt ataaagaccg ggcttgctgc cgggaagcgt taccgcgttc atgattacgc
     3901 cctgacctct tacaaccggg tctgcgccgg caacattggt aaccttgccg taaacattaa
     3961 gggtaccgtc aactattata gagccgccaa agcctatttt cagctgtccg taaacagtaa
     4021 ggcttgcgcc gggagctata ataagagttt tggtcagagt tattatctta tccgccggta
     4081 tgatatcatc gccgctgatt gtggtatcca catcaatgtc agtgtcaatc gtgtccgctg
     4141 tgacaacgaa cggtacgctg gctatcagca aaacagcgat taacaatacc gaaatcagtt
     4201 ttttcataag gcaacctcct aaaatgactt gtccgcacaa taatctaagc taagtatatt
     4261 aaataaagcg accgttgtca actgtttttg tgcattggaa acagtataga ggaggaaatt
     4321 tacacaaaaa acggtgaatt agacattaaa cggaggaata cattcgacaa tctgaatacc
     4381 ggaatttgaa gctatctcga accttccttc agccgctttg ggtaaaagga agtagtcgcc
     4441 cttctttata ggtttggagt agtcctcgcc cttaatttcg ccttcgccgt cggttacaac
     4501 ataaacagcg ggcgcggaaa gttccctaaa tgtaccgttt gtgacggtgt ggcgctttac
     4561 cgcaaagcag ggtgtgtcct ccctgccgat taactgctcg gataccacgc cgccgtcctc
     4621 ttttatgatt ttgggagctt tcttgccgag agccactgcg gcatcgccgt agacggagaa
     4681 gtcaaagcac tcaagggcgt cgtcgggatt cagaccgagg tatttctcat agtcattcag
     4741 cttatagtcg gcgcaccatg cttcgggctg gattgtaaag tcggtgggct cctgaatctc
     4801 aagaatcatg cagccgtagc cgattgcgtg gacttgtttt gcggggatga agtaaacatc
     4861 gcctgcttta gcggggattt cgtttaatag ctcctccatg gcgtcctttt cggtttcgct
     4921 acgctcaata gcctttacaa attcctcttt tgtaatcttg tccttaaacc cgaagtagat
     4981 tttcgcgttc tcccttgtgg cgaaaactat ccatgcctca gccttgccgt attcggaatt
     5041 gaagtgcttt cttgagaagg gcttgtccgg gtgagcttga atagggagcc tgacggcgct
     5101 gtcaagcatt tttacgagaa ttccgaggct gtcgtgtccg ccgaggatgt tctccctttc
     5161 cttttctata agctcgtcaa ggtaaatatc tgtgccctgt acctttgaca gaccctcgtt
     5221 cgggtcggtg gagttgctgt taagagcctt taccgcggag gcaatccact cctccgggta
     5281 gttgccgtcg gtcggctcat caccgaagaa atcgttaaac agcttgccgc ccaaatatac
     5341 tctaaaaacc ctgtttcttt caaaaaatat aggctttctt gaaatctccg aagttatgtt
     5401 ttccatctta atcctctttt tcgtacttga ttatgctctg agcctcttta agagacttga
     5461 atactcccgc ggcaacaccc gcgaagagag ccgcgccgta tgccgcctcc tccttatgtg
     5521 cggggacttt tacttccatg ccgaatttgc cggcgaacat actgcgggtg actttgctct
     5581 ttctgatgcc gttgcccgag ccgacaaggg ttgagggagg agttgctatg ttttcggaca
     5641 tataggtata tttttcatac agctcgttta cagtgccctc aagaacgcct acaaccagat
     5701 gctggggtgt aaagttatcc gcgctgatgt tggatatgga tcctctcttt gagggattgc
     5761 ctctttcacc gcagaacagt gttgaaacct ccagcttgtc ggtaagggtc ttgtagccct
     5821 cggacagctt atccataatc ggatacatgg tgaagacctc ggcgtcggtg agctgaacga
     5881 cggaacggaa gaagcgctcc aagatagcgt atgctctgcc gccgcagagg gagggaccta
     5941 caagcagata cttaccgtcg gtccacgggc gggtttctat atactgattg tataagggct
     6001 tgtcggtcac cattgaaacc tgaccgcctg tgcccacatt tacaaggatg ctgctctcag
     6061 agtctttaac ggagcccatg aagctcgcct ggttgtcgcc tatggcaacg gctacgggga
     6121 taccgtgctt tgtcttgccg atgatttcaa agcctttttt gacctcgggg aagaatgacg
     6181 ggtcaattcc cagcttttcc atagccgcct cgtcaaactt atcctcggtg agattgtaaa
     6241 gtccaaggct tgccgcgtct gacgggtggg tgacgggttc cttgccgcct gtaagcttca
     6301 tacccacata gtcatggatt gtggagagct ttactgcttt ttcggggata agggagttct
     6361 tcatattgaa gaagtgggtg acggtgccga aacccgttgc aaggtcgcag cctgtaacct
     6421 cggacatata ttccgcgtag cttttgccgt ccttgtactc ctggtctccc ctgccgtcct
     6481 gccagatata aagggggctg acagcattgc cctccgcgtc aaggtagagg attccgtgca
     6541 tctgacccga aatgccgatg gcgtctatgg gtgaatgctt ttcaatcatt gcctccactg
     6601 cgtgggtggc gcaagagaga atgcgggcag ggtcctgaat tttttcatag gctctgtctg
     6661 tatggataaa tgaagtgttc ggaacatttg aagtgcaaat aacctggcct gtttccgaat
     6721 cgatgacatt aacgcaaata gaagttgtgc cgatatccaa accaagcagc ttcatgtttt
     6781 tcctcctgtt acattgatgt tgaggcagga tcaagccttg tgataatgtc cttctgtata
     6841 gcgggcgaca aatcaaggaa gtttacgaat gtgccgtgga ttctcattat ataaactccg
     6901 ctgggggcgt actttttggg attttctaaa accttgcgga gtatctctgc cgtggtgaca
     6961 tttacataga ggtagaaagg tgatacgcct tcttttgcct cgttgaagaa caagggagcg
     7021 atgaccctct ttttgaagtc aagacccttg ccctcatagc tctcgcttgt gaagtacttg
     7081 gggtcgatgc cgaagtggga ggcatagccg ccgttcatct tttcgtacgg gagcttgaag
     7141 gcggaaaggg ttctgaagcc caggccctga tgagcgtcgc cgtacagcgg gtcaagaccg
     7201 tagttgagca tatctcttga ctttctgccg tcgggtgttg cgcctaccca gtagccgtaa
     7261 agcaggtggg ttgagggtgt gctgaagtcg gggcggaagg gattgccgag gtagttcttt
     7321 tgagacgcca caatgtccgc aaccttgttt gcgacatcag ctgcctcgtt gtccacgatt
     7381 tcctcgttat ttccgaactt gggcgcggac tggagatagc gtcttaattc ctcatcgccc
     7441 tcaaagttgt ttctgagtgc ctctataagt ctttctgcgt cctcgggtct ttcttcaagg
     7501 agcttatcga tggcgacaaa ggagtcggca acaacggata cgccgtgaat caggcagccg
     7561 ctgccgttgt actttgtgcc ctgatggtcg cagtcgcgca tatcgtagcc tctctccgcg
     7621 cagcccatga gcacgctgag gaagggaacc ctgagatttg aaagggctct ggaggcgtcg
     7681 ttcgcgctgt ccgccattat ctttgtgtat ttctcaacat tctgatagaa aagctctctg
     7741 atgttcttaa gcctttcaac ggggtccttg gggccgtggg gctcaaaaat cctctcgccc
     7801 gttactgtgg acacgccgtc gctgattgtc agctcaagga ttttgccgag attgagccag
     7861 ctgtttgtgg tgttgccgtt gtccttgccc ataataagtg gctcctgaca gccggcgatt
     7921 gagtagtcgg gcaggtcctc tttatctctg ccagctcttt cgagaacctg gaacatcgcg
     7981 tcgtcgttaa acagcgacgg ggtgagcatg ccgggtgtga agaagaattt gcccattgcc
     8041 tcataaatgc tgtcgggggt attcttgtgg agctttaccg aaagaatcgg ctggggcagg
     8101 ttcatatcat aataggcatc gaaaagggca aaggtgctct cgttggaaag gtcattgccg
     8161 tcaacatctc tgccgccgag aatgatgttc tgagaaattg cccagcttct gtcgccgaca
     8221 ttgaagaaaa caaggaaatg cttaaacaga gcggaagcaa gctctcttga aaggttttct
     8281 tttgctctgt agggctcgaa aatcctgtcg gcgttgccta cggagaatgc gaaggggttg
     8341 ggggactgct ctatgcacat tacctgccag aggataatga agctctggat tgcctcatag
     8401 agattgtccg cgccctttgc gggaaccttt gcgagggtct ttgccataag ctcaaaacgc
     8461 tctttggact ctcctgacgc cttctctgcc ttctcttttg ccagagctga atatctgccc
     8521 gcgagaataa cggctgcctc gagcgctatc ttcatagcct caaggtttgc tttgcgctct
     8581 gagtctgtct cggcgcttat tctcttttca atatcggcaa taaggctttc tatgccgtgg
     8641 gcaagggcgg gtctcatatc ggggataagg tgacccgtaa cctgctcaaa gaagtaaaca
     8701 acctcggatg tgtacttctc cgccttgctg tatgcttcgg aaagctgcct tacatagtcg
     8761 gtgttcttat tgtactcgcg catttcttcg atgtgctctg cggagatttc ctccgaaggc
     8821 tctatatcgt tgaaaacggc ggtcgggtcg cagtagcccg agaaggattc aacctcaaag
     8881 gaaggattta taagagcata gcttcttgca aaagcgtcgt cctgagtgcc cgcgaaaacc
     8941 gcgtagtcgc tgatgtcgat gggcagcttt tccgcgatgt cccttaaagt cttggcggcc
     9001 ttaatctcta tgtgctcgcc cttatacttt tcgtccgctt ccttttggat ttctttgatt
     9061 ctgaaccagc cgtcaaggcg ctttttggag cgttcctctt taaagagctc ctttgccaag
     9121 gcggttattt tatcagccgt ataaactgtt tggattgaca taagattacc ctccgaaaat
     9181 ggttatgttc tgatatgttt aattccccgc tccttaaata tgtcctcaaa catttttaaa
     9241 gtgccgggct ttatatatcc gtcattaaca gtatattcca taccgcattt aagccatttg
     9301 tccttgccga attcgtgata cgggagaagt tcaaccgaaa gtccgtcttg attaatggat
     9361 tcgaaataac cggcaaagcc taaggcgtct tcccttgcgg cgttaaagcc gttgacaagg
     9421 gggattctta caagcacgct tttgcctatt ttaagggctt gctctatatt agcctttgtg
     9481 ttctcgttgc ctacacctgt gacttccttt tgctttttac tgtcatagtg ttttaggtca
     9541 atgattagct ggtcgatata gggcattagt tcggggagtt tcgggcttgt gccgttggtt
     9601 tcgattgccg tatgtattcc gtttgcttta agccccttat agacttcctt aagcgcgtca
     9661 aactgcagcg tcggctcgcc gcctgtaaag gtaacgcccc cgccgtcaaa atacatcggc
     9721 ttacatttta cagcttccgc aattatatcg tctgtgctgc ggtcactcgc cgcagtgtat
     9781 agtcccaaat ccacgcccga ctgaaggcac cttctgtcgg ggcatttttc gcagcggctt
     9841 cggtcaagct ccttgtcttt gaccgctccg taggggcatg cgctgtcaag cagcttttct
     9901 ttgtttacca tgatgggggg aactacggga atgccttcgg ggttcgcgca ccacgggcag
     9961 cgcatattgc agccctgaag atgaaaaaca agcctgttgc cctgaccatc ctgagaataa
    10021 ttaaagcctt tttgaaaaac ggtaattctc acctgtatct cccgtctatc gacaaattat
    10081 tacaatattt ttgaatagat atacagtatt tgttctcaaa gaaacaaatt tccaatcgcc
    10141 ttttttttat tatagaccgc ggcatgtaga aatagcaaga gtaatttagc tcttattcca
    10201 tttttcatac atcgtcggac aggcgctttc gacaattcac ataagaaaac cgccttattt
    10261 ccgttatttg gcatgaaaaa gcggcggcat aatttgccgc cgcggaagct ttttatgcaa
    10321 aaacataaat tcccattcaa tttccgaaaa gggcgtcctg cattgctttg agttttcgtc
    10381 ttctttcggc tgtcattttt tcgtcaacgg aaaagctgcc gtcatcgttt tttataagaa
    10441 cgctgccttc ctttgcgttt ttgggcagca gtgagcggtt tacttctatc attttgccgt
    10501 catcgtcttc gattacggcg atgctttcct caaacctgtc tattatatac attcgcggcc
    10561 tcctatcgct ctgttttaaa ctccagagtc tttccgtcgg ttattacggt tatgttgccg
    10621 ttgtagtctg ttctgtagta tttgatatta tgcttttcaa gtctgccgat tgtatccttt
    10681 gacgggtgac cgtagctgtt tttcagaccg caggagatta cggcgtactg gggacttact
    10741 tttgagagaa actcctcggt ggtggatgag cggctgccgt ggtgaccgac tttaagcacc
    10801 gcggctctta cattcatgtt gtttttaagt atactttgct cagccttttt ctcggcgtcg
    10861 cctgtgaata aaaatgaggt ttctttaaag tccgccctca gaactacaga aaggttatta
    10921 agctctgccg tgtcggtaaa cggggcaagg attgtgattt taagattgtt taagctatac
    10981 tccttgccgg gaaccgccct ttcaacctta gcgccggatt cggacagagc ctgaagggat
    11041 ttctcgtaaa tccttgttgt cggcatggac gcttcggaga tgtagggcat tattactgta
    11101 tcgactttaa tgcgttttaa gatggcgggc atggagccta tgtggtctga gtgggggtgg
    11161 gtcgcgatga gtaaatctat tttgtcaaag ccgcttttat gtatgagatt taagatttta
    11221 tcctcgttcc cgctctcgcc cgcgtctatg agcattagtt tgtcgccgca ctgtatcagc
    11281 gtactgtccc cttgccccac atctataaag gtgacggtca gttcctcgga gtcggttacc
    11341 gggactgatg tttcctcaac taaggcggcg ctttcgataa catcctcggt agtggggatg
    11401 tcaccgttcg ggtttttcat aaaaacaaag gggactaaga tggagagaaa gagcaatagc
    11461 gctacggcgg aaagaacagc tttcagcttt ctgttatttt gcattattgc ccccgccttt
    11521 cgttatatta tgtcagcggt ttcttctctg tgggactctg tcggcgcatc atccgactgt
    11581 cttgcgttca tttcaagcca ctgagccttt gtgataagca ccttcctcgg cttgctgcct
    11641 tcggggggac ctacgattcc cctctcctcc agttcgtcca ttattctgcc tgccttggca
    11701 aaaccgagac ccagccttct ttgcaggttc gtgatggacg ccttttcggg gttggaaacg
    11761 acgaattcca acgccttcat aattaaatcc tttgtaccct cgtccaggct ggtctcgccg
    11821 tcggaggagg agctgtgctt gcccttctcc tgagccgcct gacgctcaat ctcgctggcg
    11881 acatcttcgt catagcggca ctcgccctgc tttttgacaa agtccactac cctttcgatt
    11941 tccgcgtcgg aaagatagca gccctgaatt cttatgggct tgggagcacc tacggggcag
    12001 aaaagcatat cgccgagacc taagagcttt tcagctcccg ctgagtcgat tatggttctt
    12061 gagtcaacct gagaggagac ggacagggct atgcggctgg ggatattggc cttgataaga
    12121 cctgtgatga catcaaccga cgggcgctgg gtggcgacaa caagatgaat gcccgcggcg
    12181 cgcgccatct gcgcaagtcg cattatggag tcctcaacgc tggagggggc gacagccata
    12241 aggtcagaca gctcgtcaat aactatgact atctggggca tccttttaag ctccttgttc
    12301 tcctcgcaaa gagcgttata ggctttgatg tcccttactc cgttgtcgtt gagtgttttg
    12361 tagcggtcta tcatttcggt aaccgcccag cccagtgaac ccgctgcctt tcttgcgtcc
    12421 gaaagtacgg ggacaagaag gtgggggata ccgttgtata cggagaactc aacctgtttg
    12481 gggtcgataa gcaggagcct tacttcatct ggggtggcgt tgtagagaag gcttacaatc
    12541 atggcgttaa gggcgacgga tttacccgag cccgttgtgc ccgcgataag caggtggggc
    12601 atcttcgcaa ggtcagcgca aatcggaacg ccagcgatgt ccttgcccaa agcgaccttt
    12661 aagaggctct tgttctttct gtaattttcg ctgtctatta tggagcggaa ggaaaccgtt
    12721 gccctgccgc tgttggggat ttctatgcct attgccgatt tgttcgggat gggagcctct
    12781 attctgacgc cagcgggagc ggcaaggtgg agggcaaggt cctccgcaag acccgtaaac
    12841 ctgctgattt tcactcccgg agcgggggct acctcgtacc ttgtaaccga cgggccgggg
    12901 atgatattaa gaaggcttgc ctcaacatta aagctcttaa gcgcgttaat cagtttctcg
    12961 cccgtagctt taagctctgc cgctgactgg gaagcgcttg actttgaggg aggattgaga
    13021 aggttcaaag gcggcaggac atagggcttt tcgggcttgt cctgattggg agcaatctca
    13081 tcctcaaggg caaagatatt ctcctcacgc tctttgatgg aggcttcctt ttcagcgtca
    13141 aagacggatt ttgccttcgg gggctgcggt tcgggttcgg gctcaggctc tttttcaagc
    13201 ggaatgtcta tgatgggagc cggagccttc tccttcttgt agctttcgtc aaagacattg
    13261 ggatagcccg aaatgggaat ctccttcggt tcatcctcaa agggagattc ggttgacgga
    13321 ggcggtccca aatccacatc cacatcgaat ttggggcggt ttttaagctc cacaatcttc
    13381 tccgcagata tgtctgaaac ctttttgacg ggcttgccca cggcggaaaa gagcttcatt
    13441 aaggatgtgc cggtggcaag cattaaaaat acaaagatgg tgagaaggga aattacaatt
    13501 gcgggcgcct tgctgtccgc aatgcttata agcagaccgc cgattaaagc gccgaaaaag
    13561 ccgctgccgc tcttaatgcc gctgtcggca aagagcttgt actgctcctt ggcgtcgttc
    13621 cagacttcaa acggcgggtc ggttcttgtt atgaacaata gtgaactgat gaaaaggata
    13681 attacaaaca gcgaaaggct gcctgtaaat atggtggttt ttttcttgtt gtatgtaagc
    13741 atgacggaga tgcagataag agcgaagggg tataaaaagg cggtaaagcc taagagaccg
    13801 aacctaatct cacgcatttt ggtccagatg gtgtcgggtc cgccttcaaa tatgctgagc
    13861 attaaaagaa acaaagcaaa ggcaaagagt atgattgccc aaaactgctc atttttatat
    13921 atcggcgtgt attcatcgta gggcgccaca ttttcggcag ctcgagtttt cttcttagcg
    13981 ccgctttttt ggcttgaggc tccggagccg ctctttgcgg cattgccctt gccggcggca
    14041 ctcgacgttt tttttgtttc agccataatg cttcatttcc tttcttttga aacaatacgg
    14101 ttttagagta cttctataag gctaactata atggcaaata cgccaaccgc aaagcagtag
    14161 tagccgaagt atttgaacat atccttcttt aaaagatact gcaaagcctt gatggctccg
    14221 actcccacag ccgcagccgt aataacgccc gctgcagcgg caatccagtt tatgtcgctg
    14281 ccctggctta cggcgtcttt taactcaacg gcgttggcgg cgaggattgc gggaattccc
    14341 agtatgaatg agtattttac catgtattct ctcgaaagtc caaaaataag gcctgtggaa
    14401 atagtgctgc ccgaccttga aatgccgggg aaggctgcgg cgaaaagctg ggcaaagcct
    14461 attcccagtg cgcttttggg gcttatcttt ttaggtgaaa tcatgttttt tgcgatgtag
    14521 gagcctgtta tgagaagaag ggcggtaaag ataaagcaca cgccctctgc aagtatgctt
    14581 ttgtcggtgg agaaaaccgc gagcttatcc ataagccgca tatcgcccga tacgggcaca
    14641 agaagcaaaa gaaggggcag gcaggacaga acaaacataa agagcattgt tctggtctcc
    14701 gacatatcct taaagctgaa tttgcctgtg aatatatctt ttattatctt aaagaactcg
    14761 aggaatatct cccaaattgt cttataaaaa actatgaaca ccgcaagaag ggtgccgagg
    14821 tgcaaaagaa cggtcatgag aagggattcc tcacccgttg agccgaaaaa atactgaacg
    14881 agagaaagat gtccgctgct tgaaatggga aggaattcgg taacgccctg gacaaagccc
    14941 aggaatattg ctttaagaat ttccatgtga actacttgcc tttcatatgt attttactaa
    15001 tatgtatgtg cgaaaatcgc acttttttcg tttaaggctg attcggctga tttttttcct
    15061 caccgccatc gctttcggga gcagtgtttt cctcgctctc gccttccgcc tttttaaatt
    15121 cctcaatcat gctgtaaagg caggaaagcg cgtcggaaag gctgcccaga gagtcgataa
    15181 gaccttcgtt cacagcctgc tcgcccttta atatggtgcc aacatccatg acaagctcgc
    15241 cagaagccag cataagctcc ctgaatctgt cggcgggcat attggagttg tccgcaacga
    15301 attttacaat cctctcctgt atgctctgga agtaggcaaa ggtctgctca acgcccagaa
    15361 ccagcccatt catcctaacc ggatggatgg tcattgtggc ggacggagtg ataaacgaac
    15421 gctttgccgc tacagccagt ggaacgccga tggaatgtcc gccgcccagc acaagggaaa
    15481 cggcaggttt tctcatccct gctataagct ccgcgattgc aagccccgcc tctatatctc
    15541 cgccgacggt gttgaggact atcaaaagtc cgtcaatgtc cttgctttct tccacagcca
    15601 caatttgagg aatgacatgc tcgtactttg tggttttgtt ttgcgacggc agggtgtaat
    15661 gcccttcaat ctgcccaatt atagtaaggc agtgaatgag cctgccctgc ttgcttaccg
    15721 tgacgcttcc cgtttccttt ataaggtctt gggtatcctc gggagtttgg ttcgcctcgt
    15781 catcgtcgcc tttaacctga ggagtcatgg gcgacggagc gggtgttgta aagctgtcag
    15841 cggtaggaaa tgatttatcg ttgaaattca tttttatccc tccgttttgc tattgtgccc
    15901 ttaattgcaa aaagtattag caaggatagc ctcggagagt aaaaatttgc cttagcctgt
    15961 ttacaaataa ttgtagcatt taatggattt gtcggcaaca gccaaaaaaa cttttataac
    16021 ttgcgaatat ataggattag atgtataatt acaaaataga ttcttatgca gcattttatg
    16081 cgcttaaaaa aatctaaagg taagatttta tgtctcttaa atacggtgaa tttttgtttc
    16141 cctcttcctc gggtctcgga gatattttcg cccgcagcta tgaacttagc aatataagtg
    16201 agaccaaggg tgtttttcag attgttcaca gcataactga gcactcggaa agatataagg
    16261 aattcgcggt ttttctgtgc tctaggggct atgccgtctt tgttcacgac catataggga
    16321 acggcaggtc ggtactcgcc gatgaggcaa agggctactt tggagagcag ggcggagcct
    16381 ccacccttat tgccgataca tatgagctga caaggcttat cagcgcgaaa tacgcgggtc
    16441 tgcccatatt tatgtacggt cagggcttcg gctcatttat cgcaagggaa tactgcgtcg
    16501 cccacaaata tgacttaaag ggcgcggtgt tctgcggtac gggcagcgcc tcccctaccg
    16561 tttccgtggg cagggctgtg gcaaagattc tgacagaaaa gaacggctcc gactaccgct
    16621 cggattttct caacaatatg cttctcggcg cgaacaataa gagatgcaag cccagcaaaa
    16681 gcaatttcga ctggcttaca agctccgagg aggatttgga gaaattcctt aaagaccccc
    16741 tttgctgcac cgagggtttg accgtatccg cttacgccga tattctctcc attgtgcaga
    16801 gagtaaacac aaaggattgg tttaagaata taccaaagga tttaaactta cttcttattt
    16861 caggcacgga ggaccccatc ggaaactacg gcaagggcgt gaaagaggtc tttgaccgcc
    16921 tgcaggccgc ggggcacaag aatacgctct taaagctcta tgaaggctgc aggaatgagc
    16981 ttatacacga gtgcggaaaa tactcgattt ttgacgacat agccgactgg gcggactcgg
    17041 taatagaaaa taaggcataa taaaaaagct ctcatcaacc gatgagagct ttgatttttg
    17101 tttaacggta tttcggcagc caggagccgt gtgcctcgat aaggtcatcg cagagcttta
    17161 cgatgtcgtc tattgaaagc tctgatgagc agtgcgggtc aagcatagcc gcctgataga
    17221 tgtgctcctt cttctgagtt acggcagctt cgattgtaag aagctgaaca ttaatcatgg
    17281 tcatgttcat tgcggcaagc tgtaccggca gcgggggctg cttgcagggc tgaacgccga
    17341 gcttgttgac aaggcaggca acctctacgc atgcctcttt ggggaggttc gggataagac
    17401 cgtcgtttat tacattgccg ccgattttgt acggagtgtc ggtcatcatg gagttcataa
    17461 tgcccgatgc gtactcgtga gtcttcttgt ggctgacctc gtcgttgagg tacttcagct
    17521 tgtcgttctt ccagcgctca atctgattta cgcagcggcg gggatattcg tcaaggggaa
    17581 tgttataacg ctcgataagc tcgggatact tatccttaat aaagaagttg ttgtactcag
    17641 cgttatgctc gcttgactct gtgcagtaat atccgaacct tctgatgtac tcatatctta
    17701 caaggtcgtg atgctccttg ttgccgcttt caagaacctc gatagccctt tttctgattt
    17761 cgggatagag gtctttgccg tcggcatcat atatctcaag cagccagccc atgtggttga
    17821 cgcctgcgat tctctctttt ataggctcct tgtactctat gcccaggtct ctcaaaatgc
    17881 ctcttgagca gccctgaacg ctgtggcaaa gacctacggt cttaacgcct gtgtatctga
    17941 gcatatagcc tgtgagcatg cccatggggt tggtgtagtt gaggaaccaa gcgtcggggc
    18001 agacctcctg catatcctcg gcaaagtctt tcagaacggg gattgttctg agggctctga
    18061 agatgccgcc gattccggtg gtgtcgccga ttgtctggcg cagaccgtac tttttgggaa
    18121 cctcaaagtc gatgattgtg caggggtcat aaagacctac ctgaatggcg ttaacaacaa
    18181 agtccgctcc tctgagggca tcctttctgt tttcaacgcc gagatatttc ttcacttttg
    18241 ctctgttttc gtttatgttt ctgttcaaag ccgtgcacat tatgtacgac tcttcaagcc
    18301 tttcggcatc gatgtcgtag agtgcgattt cggcgtcgcg cagggattcg gtgagcatta
    18361 catcaccgat aacattcttg gcaaagactg tgctgcctgc tcccataaag gtaagtttca
    18421 tataatgatt catcctttca acgcattagg caggataacc gccttttgaa gtatacaacg
    18481 aagaccattc aaggtcaaga ctgtaagggg cgcgcggatt aagatttgat ttaattgtca
    18541 atccgcgtga gagtttttta gtgattctga cagtgccgtt tatgctccgg ggattgtcct
    18601 cattgccaag atggtcttgg tgatgagctc gtcaagctcc cagcccagca tatccgctcc
    18661 cctctgaatg acctctcttg agcagccggc ggcaaagctg ggggatttgt acttcttttt
    18721 gacggattta agctccaaat cctgaacgct ctttgacggg cgcatgagcg cgaccgcgcc
    18781 gataagacct gttagttcgt cgacggcata gagcaccttt tccatgatgt gttcgggctc
    18841 gatgtcaact gtgagcatat agccgtggct ggcggtggcg tggataagct cctcgctggc
    18901 gccgttctct cttaatatct cctgcgcttt gatgcagtgc cggtctggat agagctcata
    18961 atcaaggtcg tggagaagtc ccacgatgcg ccagaactcc tcgtcctcgc catagcccag
    19021 ttccttcgcg aaatagccca ttaccgcctc gacggtgtag gcatgcttta aatgaaactc
    19081 ctctttgttg tactccttta aaagctcttc agctctttct cttgaaattc ctgcctgcaa
    19141 tttttaaact cctctccaat ctattaaagc aaggctattg cctctatttc cacaagcaca
    19201 tctttcggca gtctgctcac ttccacgcaa gctcttgcgg gtttgtgggg gaaggcgttt
    19261 tcgtattcct tgttaaaatc ggtaaacatg gacaagtcct ttaaaaagca ggtggtcttt
    19321 acgacctttt ggaccgatga gcctgccgcc tcaagcaccg ccgcaagatt tttcagcgcc
    19381 tgacgggttt gggccgctat gccctcgggg atttcgcccg tatcggggac aagtgggatt
    19441 tgtccggaaa cgaaaaccat atcgctcgct actatcgctt gggagtaggg acctattgcc
    19501 tttggggctt tttcggttga aacctctttc atggcgtttc ctcctttatt aaggcttgta
    19561 ttgcaatctt attttgccag caaatccttt ataacctcgc ctgcgattat gagtcccact
    19621 accgacggaa caaaagctat gctgccggga atctgccttc ttgaaaggca ggttctttcg
    19681 gtgccgggcg ggcagacgca gttctttctg cacgcctcaa aggggtctct gggcgtcatg
    19741 ggcggctcct ttgaataaac cactttaagg cttttgactc cccttttgtt aagctccctt
    19801 ctcataaccc tcgctagggg gcagacggag gtttcgtata tatcggcaac ctcaaacttt
    19861 gtggggtcca gcttgttgcc cgctcccatt gagctgatta tcgggactcc cgctttgtac
    19921 gctctctcgc aaagctccag tttcgcagtg actgtatcga ccgcgtcaat tacataagaa
    19981 taaacggaca aatccacttc gtccgcgttt tctggcagat aaaacattct gtgggcgtta
    20041 actttaacat tagggtttat atcgagcatt ctctcttttg caacatcaac cttgtatttg
    20101 cccacggtgc tgcgcagggc gaaaagctga cggttgaggt tagtaaggca tactttgtcg
    20161 tcgtcgaaaa tatccacttc gcccacgcct gttctgacaa gggcttcaag ggcaaagctg
    20221 ccgaccccgc ctatgccgaa aacggctatc cttgagtttt taagcttttc catgccttcg
    20281 gggccgataa gcagctcggt tcttgtaaat tgattcagtt ttcatcagct ccgtataaaa
    20341 agttaaagcg gaggaaaacc tccgctcttt attttagtaa atactttcta aattatcaat
    20401 atctttatgc tttgcccgaa actttgttaa aggcgttttc gatatcggct aaaatatcgt
    20461 caatgtgctc agtgccgatt gagaacctta tggtgttggg cttaatgccg ctctccaaaa
    20521 gctcctcctc tgtcatctgg gagtgggtgg tgcttgcggg atggattact agggatttta
    20581 catccgcaac attcgcaagc agggagaaga gctcaaggga gtcgatgaac ttcttagcgg
    20641 tttcggcgtc gcccttaatc tcgaaagtga agattgtgcc gccgccgttg ggataatatc
    20701 tcttgtaaat ctcgtggtcg ggatgggttt cgattgacgg gtggtttacc gcctcgacaa
    20761 gcggatgatt cctgagatac tccacaacct tgagagcatt gtagacatgg cgctccactc
    20821 tgagggacag ggtttcaaga ccctgcagga aaaggaagga gtggaagggg ctgagagccg
    20881 cgcccgtatc tcttaaaagc gtggtccttg cctttatgat gtaggctgcc ctgccaactg
    20941 cctctgtaaa gacaacgccg tggtagctgg gattgggctt tgagagtccg gggaacttgt
    21001 cgcttgcggc ccagtcaaag ttgccgctgt caatgattac tccgccgatt gcggtgccgt
    21061 gtccgccgat gaactttgtt gctgagtgaa ccacgatgtc ggcgccgtgc tcaatgggtc
    21121 tgaaaagata aggtgttgag aaggtattgt cgactataag cgggatttcg tgcttgtggg
    21181 caatctcagc gaccgcgtcg atgtcgacta tggttgagtt ggggttgccg atgctctcaa
    21241 cgaaaatcgc gcgggtttcg gggcgaatgg ctgcctcgaa attctcgggc ttgtcgccgg
    21301 atacaaaggt ggtt
//
LOCUS       DAONVQ010000016        20561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_1879_length_20561_cov_4.475422, whole genome shotgun sequence.
ACCESSION   DAONVQ010000016 DAONVQ010000000
VERSION     DAONVQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 20561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20561
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1879_length_20561_cov_4.475422"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2746)
                     /locus_tag="PLD98_02140"
     CDS             complement(<1..2746)
                     /locus_tag="PLD98_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05084.1"
                     /translation="METKHSNTKVAKKSISILLSVLMVLSCFSVAFPTIAKAAATTQQ
                     INELKSALAAFVNSGETGSYSVTGNMANGNYHIYDNTSQGYVYNVAVKLAPVFAGEMS
                     TATGSGYNWWTLMRSRIKSLTGYSSGVGATLIDNLLPVISSYDYNNKYYQESGLWGST
                     PDRALTAYGNLYVRVSRTVNAALMSYANLSQVPNNLDITFQYYCDTAEPTETGEKGGT
                     FNKRKWRREWYAFGSGLSYGVSAQDTTTAPAIKAAYNHFTPALVGLSTYDLTVQYDAS
                     TIASLINTNNSYWSTVNSLATAIKNKFFTNPTVNDIQAFMGRAVIAQQVISLLPANTW
                     YLNAMAAGYDTQNYSQMTSLYSTAVPYYNSLNGASADAKAVLTTNYSMNLNAFNAWKE
                     RLFDDIQIYELRELKNTIDQKIAEVNQYKIPDEQYPIYKPHVYFGDANTELTNTYLDV
                     ASGQFAGWLSAIGPYQQENINKVFPEGTGYIQAVLASINYEIQVRNYESVYAPFYEYF
                     TPWFAKDLTEVNSTYIIEKLYPEVIAKQSAFNTQYNNAVNILGQEAVNIIFGTLKTDI
                     VDFIDKMHKELEARITAEVNLAYSYYTAFNQITYQNFTLVKDAIGRVETNIYDFLAPT
                     AYMTQDTRNKYNALFGTILQQYNNFVNTGGFSIYQQQTFDGGTGIYYVRTPMEQDLAR
                     TTEYRVTEQRLLDTLNKLDDLLQSPDFAELVGLDGTVEQMVLDLLADELFSDKTVNEL
                     VGLLYPMLVEMFEVDLIGSIPSSIDTGTIFGSVGIGLRYTPRELCNTIGLRVYPDQFA
                     TLIDSSFAQVKNELASKTSWTPLLDEDDNLNLNWGVDEAEGYHAKKARFMSAVDQALR
                     GVLPLFRVLLCNYTWRVSINKVGYGEKDLGILGSIKINVDLDLTADGCHGYINTLAPI
                     L"
     gene            complement(3742..11376)
                     /locus_tag="PLD98_02145"
     CDS             complement(3742..11376)
                     /locus_tag="PLD98_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05085.1"
                     /translation="MGKKILSSMLALIMVLSCLSAAFPMMASAAASSSQISALTSAIS
                     AYVNSGQTGSYSVSGSAENLTYTVSDNTTLGYIYNIAVALAPVFANEMSTASESGNNW
                     WTIMRNRIKSLTGYTSGAGATIIDVLLPVVSAYDTNNRAEKEKSFSTPDPFLPSYGNI
                     GVKVTRTVSAAILSYSTLESLPSVIDIAYQYYCGTAIGNKTGNSGIKRYRKQWYYFGS
                     GLTYGVAQQDSTSTANIKAAGNYFTTSLVGQSTYQLASGNNASSIATLINNNNYYYNV
                     LMGISADLRNRFFTNPSVTDIQNFMNRAVAAQTVIAMNPANTWFQNAIANGYNNQDYN
                     GMKSLYSTAVSYYNSLAAVTGEAKTVLQNNYGMNLTQYNAFKEQLLDDIEVYELTQLK
                     NAIDQKKAELEQYRIPADQLEQYKPHLFFGDASTALTNIQLDVASGQFAGWVSTLGNY
                     QAENINKVFTSGTAYVSSMLSDLRYEINVRNYETVYAQFYEFFIPRFGADLTAIKSEV
                     IYGTLIPEAQSQRTAYNNHYNTAVSLIGQDAVNIIFGNFGVQINDYIDRMYRELEARI
                     TAEVNLAYSYYSTYNKITYQNFTMVKDAIGRVETGIYNFLASTAYMSQDTRNKYSALY
                     NSILNQYNNFVATGGYSIYQQQTLDGGTGIYYVRNPMGGDLTRDPSDPNSAYRVTEQR
                     LLDTLKKIDDLLLSEDFLKLTGSDEKLDDMLKNMLAEELFTDESVNKIMSTLYPTFAE
                     LLNEMIADIPSSYRYEKGIVRVTIDINILKTPAIIAQDFGLQVFPNQLASKINASMFP
                     DVYNALNSNSSWTPLLNEDEELELNWGIDELEDYNAKKTRFMNAFAQSLKGIEPLLRV
                     LLCNYTWKAGSSSVAEGEKSGISIKADVDLTAYGNHGYINTIAPILEAIGCNPSLIAN
                     ASAMQNITTTEGIVNAIFNPILDFIENKLAKAPLDTIVSMLPNLAYAISFDRIKPLLN
                     NLGTSIYYYIESHSLSIKLADGTEAFNVGDSLDLDDMDLDLTSLNGLLSGFLFDDDDE
                     EAPAQLPLLNTGLLAFMGQLQVTSSVRQVSMTGLPAGQRYNIVADKADVFYYLLSYLL
                     EAISDPVVREKLLGAFSDDDEEEEVVVQQAAGAQVFSAFAAAGFTAFGADDEEEDEVD
                     ILDIILQNLGLDLSAWDQYGDDYDWGFEDGDREGFMNALYTLLAPLTPVLKAFLADQN
                     LNLIVGKNNNPFISLNGYKGYAYGIVPLLEALGVKNLKSQAQYEAAVAADPTAALRLI
                     IDPLFDRLNEIAADPINEILQILPALLHYIDCGGIQTTVNNILYPLYAIIDTLRPVYA
                     IDLDTVLKDGFELDLDEIINGLANNPQAAIAVIVELLNPQDYSMKTPNWKYTDQGNGD
                     NVSIVYLGYGTRWTKAKAQDVLNNIDIIVNDLIKDEDEGIGSLSDYIMNAINGLFTVD
                     TIRSLAETLRGLSESLFGDDEDEEEPTEPEEPTDTTAPTTQPTTAPTEPTAPTETTAP
                     TTSAPTEPTTQASAEPEEPEDEDEFEFSFELLNSISLDWLLAFLESQLDADLEFAEIV
                     GRGLKSIFQNVAVPYTSANGTTAYTTYGALDNADVFTCVLSILLEVVTYGDNAEVLDE
                     MLETDGLLPAVTGLFAGRINDMAEIDWLWFTDDIEGPITGDTEIELPERSIVFLTYPN
                     NWTKATANYLDAHLAEIVDSVIRTSGDYASLAEMLSEKVDFYNDDTVNSLLKALKDIT
                     AEIDRVLLETVGVVLELDLTAWDAYEEDHVWGVTDRESFVAAVCEVLMPLAEAVDWLL
                     YGDDYTFFEGFDLTGQGGKNLITIKGADGFAFGIVPLLEAIGCKNILNYVDIKAELES
                     NPESTVRFTAILNPILDRLEEILANPVDELLGIIPNLIFFINANGLSVSIKNTLHAVL
                     NILEQVGPAADISLEELIGFSIDDLTFTGIFEILKAETGIDLNAPLGEFLSTFWLGDL
                     TVYTSANGTGAIRMDYSEDEHRGDLITIVFCLLIHTIQHEANSEKFREMLGDETYDAI
                     LNVLNLREFPMQDIRWYNTEYADTDTVFNAIESSQLFEYGYGSMWTKQKATYIANNIG
                     DVVDNIVKLLGPEGDNGQIITSLDELIADLANGNIYTSDTVNSLVETLQDFVAQIDEL
                     GEAGEHAKALIYEIFGIDLDHWKQFGPDYDWGFENGDRAAFIAAVAEVLSPLNPILEW
                     LLCDKDISFFVDENGDNAVTFLGAEGYAYGIIPLLEALGCQNILTPAEYYAAVEENEN
                     NILIAILTPLFAKIDDILSDPANKILEMLPGIIYFVNSNGLDTAFKNILHPVYGILHA
                     IEPVVQIDLYDMLGIRLDEYNFESLYKLALSSIAESTGFSFDELVGNAINELALGTIV
                     SFQSKNGQTAYTMVYAPQADQGDMVTILLRLGIKFLTYGNNMETVKTMIKERDMFSPA
                     SRKYVLALLETMVELMNEPRGVDATMGVLYYVFIGAERASDGATDILDQINKEWKAII
                     KELLNSDSELIKNIGKAISDLLNENFDDIFDEGGLASNGLLKFFAKIREFFEMISNFF
                     KNLLKF"
     gene            complement(12061..12444)
                     /locus_tag="PLD98_02150"
     CDS             complement(12061..12444)
                     /locus_tag="PLD98_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HQA05086.1"
                     /translation="MACDVRFFVCKHCGNIVGLVHNGGGPLSCCGEEMTELIANTVDA
                     SLEKHVPVAKVEGNKVTVNVGEVDHPMLEEHYIMWIYLKTDKGGHRICLKPGEAPKAV
                     FALADEKPIAVYEYCNLHGLWKAEI"
     gene            complement(12587..15163)
                     /locus_tag="PLD98_02155"
     CDS             complement(12587..15163)
                     /locus_tag="PLD98_02155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQA05087.1"
                     /translation="MDLKVRWIEKGGDFSDAAKVRKRVFVEEQSYSLEEEFDSLDSVS
                     EHLVLYDKDKEIACGRLIDLGGGAYKLGRIAVDKEYRGRGLGLMLVNLLSEKAEEKGA
                     TRLLIGAQTRVVPFYEKLGFVPYGLEYMDGHIPHVDMAKCLDFKDCRWLMFRKNAEAF
                     LARKTIYLTKKVKSAVLRICTLGFGEFYVNGQRITDDLNVPAWTNYEYRDLSKIHMPI
                     YDTLTHRVYYLEYDITSFLKDGENALGVHVGNGFYGQHESRNEGFTRCGDLKLAFSIN
                     LIYEDGELERIVSDSTVKVYESYILRTNIYFGEIQNLNNEPEGWNDAGFDDSEWYPPA
                     LADAPKSILEKQECPPDRVIRTIQPKLISKKGDHSVYDLGENVAGYPVLKFPEKSRAN
                     ETALLRIAEEINEDGTLYFFSAGDIHRIQQDFYIHNGKDDSLYYPRFTWHAGRYFEII
                     GTAEPLEFRVIHTDIKNTSEFESSDDLLNWYYEAYIRTQLNNIHCCVPSDCPHRERLG
                     YTGDGQIASGACMTTLSAKEMFKKWMKDIADNQDRFGGHVQHTAPFYGGGGGPGGWGG
                     AIVIVPYNYYRYYGDTDVLREYYPNMVKYIEYMVSRCEDNLVVREEEGGWCLGDWCTP
                     HNIILIPEPFVNTYYLIKTARITLEVAKILGINKDNEYLNKVIDDCSKAMHDNYFDEK
                     TGSFLEGIQGADAFAADLGLGDERTLKNLVDKYTQIGRFDTGIFGTYILLDVLFKNGY
                     GELAYKLLTNKSKVSFHRMRESGATTLWEEWEGRHSHSHPMFGGSVEHLFSYILGIKN
                     TEGTVGFKDVTIAPADIPSLSYVKGSMLTENGRITVEIDRRNGETRIDAKADDGIIIR
                     RA"
     gene            15351..16127
                     /locus_tag="PLD98_02160"
     CDS             15351..16127
                     /locus_tag="PLD98_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQA05088.1"
                     /translation="MAEPKLGVQLYTLRDYIKTYEDTDRTFAFLQELGVNIIQISGIG
                     DFPAIEQAELVRKYGMQVCVTHKSFERMQNDLDALIDEHLLLGCDSIGVGSMPVSYRG
                     SLEGAKAFIKEAERIGKHMRKRGVRFNYHNHDFEFETYEGRRIMDVLIEESDPEYVWF
                     VPDVAWIQIAGENPAEYIKKMQNRIKVLHFKDFIIDEDGRRFTEIGLGQCDLKACYKT
                     CVELGVPYIVYEQDSGFAVNPLESTKVSFENMKKIAAGAL"
     gene            complement(16128..16457)
                     /locus_tag="PLD98_02165"
     CDS             complement(16128..16457)
                     /locus_tag="PLD98_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05089.1"
                     /translation="MLRPRKLVVSGALLLCALTLPIAITQTLGVGGGDISEYHSAETT
                     AQGTKYTVRAYEEKIAVFVEDETVPSQILDVFLFSLPPADAEILENGITVYGEESLAR
                     VIEDYTS"
     gene            16675..18912
                     /locus_tag="PLD98_02170"
     CDS             16675..18912
                     /locus_tag="PLD98_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQA05090.1"
                     /translation="MGESRKKQNPLAGIKLERNKKQKAKKHHSKAFNTFLTVILSLAI
                     IGIISVTSVAIYVLHDIVSIANGDVVIDLDEYMLNQSQTTILYAYDENGKEVEIARLH
                     GNENRIWVPLEEMPKSLIDAYVAIEDKRFWDHSGVDWMRTLSVVVKYRFSQGGSTITQ
                     QLIKNLTGENGRTFIRKYREICYALNLEKHASKEKILETYLNTLYLDEGCYGVQTASE
                     FYFGKDVSELNLAESACLAAITTAPRTYNPIRNFENNRRRQKLCLDYMLEQGKISKEE
                     YDEALNYEIIFTNSEKYVPKKDNKTTKEKKDTYQSYYVDYVIDKVIADLQEQYNYTYN
                     QAWRKLYYGGLKIYTAEDMNIQSVLEDIYYNRKTLPKGTKDGQAVQSAMTVMDYQGRL
                     KGIVGRAGPKPGDRSLNIAAKSPRQPGSAIKPLSVYAPAIEKNYAHWSTLMQNYGLVL
                     KDGSIWPKNYDGPGSPGSYKTIADAIPPSLNTVPARLVDIMTPKVCYDFLVNNFHIST
                     ATTSDVNLAPLAVGAMSKGVTTLEMAAAFATFGNGGKYYEPICYYRVTNHDGSKVYLQ
                     TQAKPEQVISEATADIMLRLLERVITTSNGTGRKYGVSGFETFAKTGTTTDNKDRWFV
                     GGTPYYVAAVWLGYETPKDLSHISGNPAGQTFKTVMDEIHKGLEKKGFTHSEDVVKKV
                     FCTRTGLLASEYCTSKSTGWYKFDHVPSTCTTCKYSSPQVNPGGVTTEPEAGEGNEEQ
                     PNPEQ"
     gene            18967..19383
                     /gene="ruvX"
                     /locus_tag="PLD98_02175"
     CDS             18967..19383
                     /gene="ruvX"
                     /locus_tag="PLD98_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQA05091.1"
                     /translation="MVILAVDYGEARTGLAVCDKNEVLATPLSPVVVKSTDMLIDTII
                     ETAKQYKAELIVVGLPVNMDGSHGFRAEAVKEFAQLLKEKSGMPVEFWDERLTTMSAS
                     RLLNETDVKGKKRKMKLDSAAAALILQSFIDSRKRG"
     gene            19559..>20561
                     /locus_tag="PLD98_02180"
     CDS             19559..>20561
                     /locus_tag="PLD98_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HQA05092.1"
                     /translation="MASFDIDPFRDKNSERYMLSKRIGKLAEVLYKDYLYTDTEPLGG
                     FYYRDGQYSPAQINEGEWVPFDSENGWWGYPECYCWFKHSFKVPERFKGKTVVYRLYP
                     SDSGEWNVNPQLIIFIDGKTVQGADPNHREVRLLDKAQGGETFNIQINAYTDPFAWRG
                     PVRMRAKLHTLDEDVLKLYYHIKTPLDAANLLGPDDLARIEIVNKLNTAVSMIEFGAP
                     ESEFKKSVLETIDYLEENLFGKALDGVEVSAIGHTHIDVAWLWRLRQTRDKTGRSFAT
                     VLKMMDEYPEYKFMSPQAQLYDFVKHDYPDVYEGIKQRVREGRWEVEGGMWVEADTNI
                     "
BASE COUNT         5201 a         4764 c         5096 g         5500 t
ORIGIN      
        1 caagtatcgg agccagggta ttgatgtaac cgtggcatcc gtcagctgta aggtcaaggt
       61 caacattgat cttgatgctg ccgagtatgc caaggtcctt ctcaccgtag ccaaccttgt
      121 tgatgcttac tctccaagtg tagttgcaga gaagaactct gaacagcggc agaacgcctc
      181 ttaatgcctg gtcgacagcg ctcatgaacc ttgccttctt ggcatgatag ccttcagctt
      241 cgtcaacgcc ccagttgagg ttcaggttat catcctcgtc aaggagcgga gtccagcttg
      301 ttttggaggc aagctcattt ttgacctgtg cgaatgagct gtcaataagc gttgcaaact
      361 ggtcgggata taccctaagg ccgattgtat tgcacaactc tctgggtgtg tagcgcagac
      421 caatgccgac gcttccgaag attgtgcctg tgtcgatgga gctgggaatg ctgcctataa
      481 ggtcaacctc aaacatctca accagcatcg gatagagaag accaacaagc tcgttaacgg
      541 ttttgtcgga gaacagctcg tcggcaagca ggtcaagaac catctgctca actgtgccgt
      601 caagaccaac aagctccgcg aaatccgggc tctgaagcag gtcatccagc ttgttaaggg
      661 tgtcaagcag tctctgctct gttaccctgt actctgtggt ccttgcaagg tcctgctcca
      721 tgggcgtacg gacatagtaa atgcctgtgc cgccgtcaaa tgtctgctgc tgatagatgc
      781 tgaagccgcc ggtattaacg aagttgttgt actgctgcag gattgtaccg aacagtgcgt
      841 tgtacttgtt tctggtatcc tgcgtcatgt aagccgtggg agcaaggaag tcgtagatat
      901 tggtttcaac cctgcctatg gcgtccttaa ccagtgtgaa gttctgatat gtaatctggt
      961 taaatgctgt gtagtagctg tatgcaaggt tgacttcagc ggtaatcctt gcctcaagct
     1021 ctttgtgcat tttatcgatg aagtcaacta tgtcggtctt gagtgtgccg aagataatgt
     1081 ttacggcttc ctgaccgagg atatttacgg cattgttgta ctgggtgttg aatgccgact
     1141 gctttgcgat tacctcagga taaagctttt caatgatata tgtggagttg acctctgtaa
     1201 ggtcttttgc gaaccaagga gtgaagtatt catagaacgg ggcataaacg gattcgtagt
     1261 tacggacctg aatctcgtag ttaattgaag cgagaacagc ctgaatgtaa cctgtgccct
     1321 ctgggaatac cttgtttatg ttctcctgct gataggggcc tatagcggag agccagccgg
     1381 cgaactgacc ggaagcaaca tcgaggtaag tgtttgtcag ctcggtgttg gcatcgccga
     1441 agtaaacatg gggtttgtaa atcggatact gttcatcggg aatcttgtac tggtttactt
     1501 ccgcaatctt ctggtcgatt gtgttcttca gttctcttaa ttcatagatc tgaatgtcgt
     1561 caaacagccg ctctttccat gcattgaatg cattcagatt cattgagtag ttggttgtca
     1621 gaacagcctt tgcgtcagcc gaggcaccgt tcagtgagtt gtaatacgga actgcggttg
     1681 agtagaggga tgtcatctgg ctgtaattct gcgtgtcgta tcctgcagcc attgcattca
     1741 gataccatgt gttcgcaggc agcagtgaaa taacctgctg agcgatgact gctctgccca
     1801 taaatgcttg aatgtcattt acagtcggat ttgtaaagaa cttgttcttt atagcggttg
     1861 caagactgtt tacagtgctc caatagctgt tgtttgtgtt gataagcgaa gctattgtgg
     1921 aagcgtcgta ctgaactgta agatcatatg tagacagtcc gacaagcgca ggtgtgaagt
     1981 ggttgtaagc agccttaatt gcgggagcgg ttgtggtatc ctgagcggat acgccatagc
     2041 tgaggccgct gccgaatgcg taccattctc tgcgccactt tctcttgtta aatgtgccgc
     2101 ccttttcacc tgtctcagtc ggttctgcgg tatcgcagta gtactggaag gtgatgtcaa
     2161 gattgttggg aacctggctc agattagcat aggacatgag agctgcgttg actgtccttg
     2221 atactctgac atacaggttg ccgtaagcgg tcaatgcgcg gtcaggtgtg ctgccccaaa
     2281 gaccactttc ctggtaatac ttgttgttgt agtcgtagga tgaaattaca ggaaggaggt
     2341 tgtcgataag ggtagcgccc actccggatg agtaaccggt aagggactta attcttgacc
     2401 tcattaaagt ccaccagtta tagccggagc ctgttgcggt actcatttcg cccgcaaata
     2461 caggagccaa cttcacagca acattataaa catatccctg cgaagtattg tcgtagatat
     2521 gatagttgcc gttggccata tttccggtaa cagagtagct tcctgtttca ccggagttta
     2581 caaaggcagc cagcgcgctt ttcagttcgt taatctgctg cgtggttgcc gccgctttag
     2641 ctatcgttgg gaacgcgacg ctaaagcagg aaagtaccat aagcaccgat agtaggatgc
     2701 ttatggactt tttagcaact ttggtgttgc tatgcttggt ttccataaac tcactccttt
     2761 aataaaatgc tgggtttgaa ttgacttgaa ttgggtacag ttttggcaca ttacctaaaa
     2821 attgcgcagt taggcttata ttcatattaa agtatactgt tgccgatgct taaagtcaag
     2881 gcgaaaatgg atttatattg acatattgtt aatatttgca aatttgttaa catttgtaac
     2941 ataattctaa aatatatggt tttgcgttgt atcaccgtaa atttagtgcc ttcgccgttt
     3001 cggggcgaat caagtttgtt aaatccgcgt tcacagctta ttaataattt caaggtcagc
     3061 ggggctattc ggttttgttc actgaatttt aaaatatatg caaaaatttc ctttaatttt
     3121 ttatttagga aaaatgtcgc ataccattac atgattataa agaacgctgg ttttttatga
     3181 gtttcgaaca agtcataatt tatgtagatg tgtatttgtt agaatttcac aaaaaaatac
     3241 ccctctatat tctaaaatat atcacgaatt aacataagtc aagtagtttt tcccaagcct
     3301 ttcattttct atactgtttg gcttgattga cgcaaatata ttttagaatt ataagtttag
     3361 agcgggaaac atggaaagct gccggagctt ttcccattta ttatcaaaat ttataggatg
     3421 cagcatgacg aggcagactt ttgtcagaca cggagaaaat aaatacatac gctaattcta
     3481 atacaagcca aaagaaatat aaatggctaa agttttcctg aaatttgtaa atgtaatttg
     3541 ctttacagca tgactggaac gattggcgga caaattttag aacttgcgcc tccaacgaaa
     3601 aatccaagaa gtcttgcttt gtcttgaaga cattttttgt caaggcattg agctttacaa
     3661 aaacctgcca aatcacgaca cataatcaaa accaaaagaa aaaccccctg gaccgtttgg
     3721 ctcagggggt aagtgtttgg cttagaactt taagagattc ttgaagaagt tgctgatcat
     3781 ctcgaagaat tctctgatct ttgcgaagaa cttcaacaga ccgttggagg cgagaccgcc
     3841 ttcatcgaat atgtcatcaa agttctcgtt caggagatcg gagattgcct ttccgatgtt
     3901 ctttatcagc tcagagtcag agttcagaag ttccttaatt attgccttcc actctttgtt
     3961 gatctgatca aggatgtcgg ttgcgccgtc agaagctctc tcagcgccga tgaatacata
     4021 gtacagaacg cccattgtag catcaacgcc gcgaggctcg ttcatcagct caaccattgt
     4081 ctcaaggagt gcgagtacat actttctgga tgcggggctg aacatgtcgc gctccttaat
     4141 catggtcttg acagtctcca tgttgttgcc gtaggtcagg aacttgatgc ccagtctgag
     4201 gaggatggtt accatgtcgc cctggtcagc ctgcggagca tagaccattg tgtaagcggt
     4261 ctgaccgttc ttggactgga aggaaacgat tgtaccgagt gccagctcgt tgattgcatt
     4321 gcctacaagc tcgtcgaagg agaagcctgt gctttctgca atgctgctga gtgccagctt
     4381 gtagagggac tcgaagttgt actcgtcgag tctgatgccg agcatatcat acaggtcaat
     4441 ctgaacaacc ggctcgatcg catgcaggat tccgtataca ggatgcagga tgttcttgaa
     4501 tgcggtgtcg agaccgttgg agttgacgaa gtagatgatg ccgggcagca tctcaagaat
     4561 cttgtttgca ggatcggaga gtatatcgtc aatctttgca aacagaggag tgaggatagc
     4621 gatgaggatg ttgttctcgt tctcctcaac tgctgcgtag tactcagccg gagtgaggat
     4681 gttctggcat ccgagagcct caagcagcgg gatgatgccg tatgcataac cctctgcgcc
     4741 gaggaaggtt acggcgttgt cgccgttctc gtctacgaag aaggagatgt ccttatcgca
     4801 gagcagccac tcaagaatcg gattcagcgg tgaaagaacc tcagcaactg ctgcgatgaa
     4861 tgcggctctg tcgccgttct cgaagcccca gtcatagtcg ggaccgaact gcttccagtg
     4921 gtcgaggtcg atgccgaaga tctcatagat gagagcctta gcgtgttcgc cggcctcacc
     4981 cagctcatcg atctgagcta cgaagtcctg aagggtttcg accaggctgt ttacagtgtc
     5041 tgatgtgtag atgttgccgt tggcaaggtc tgcaatcagc tcgtcgagag atgtaatgat
     5101 ctggccgttg tcgccttcgg gtccgaggag tttgacgatg ttgtcaacaa catcaccgat
     5161 gttgtttgcg atgtaggtag ccttctgctt tgtccacatg ctgccgtatc cgtactcgaa
     5221 gagctgtgag gactctatag cgttgaatac ggtgtctgtg tcagcgtact cggtgttgta
     5281 ccatctgata tcctgcatcg ggaactctct gaggttcaga acattgagga ttgcgtcgta
     5341 tgtttcatcg ccgagcatct ctctgaactt ctcggagtta gcctcgtgct ggatggtgtg
     5401 gataagcagg cagaatacta tggtaatcag gtcgcctctg tgctcgtcct ctgagtaatc
     5461 catgcggatt gcgcctgtgc cgtttgcgct ggtgtaaact gtaaggtcgc cgagccagaa
     5521 ggtggacagg aactcaccga gaggtgcgtt aaggtcgatg ccggtctctg ctttgaggat
     5581 ttcgaagatt cctgtgaagg tgaggtcgtc gatggagaat ccgataagct cctctaagga
     5641 gatgtcggca gcgggaccaa cctgctcaag gatgttgaga actgcgtgga gtgtgttctt
     5701 tatggaaacc gagagaccgt ttgcgttgat gaagaagatg aggttcggga tgattccgag
     5761 gagttcatca acgggatttg cgaggatctc ttcaagtctg tcaaggatcg ggttgagtat
     5821 tgccgtgaac cttactgttg actcaggatt gctctcgagc tctgccttga tgtcaacata
     5881 gttcagaatg ttcttgcagc cgattgcctc aaggagcgga acgataccga atgcgaaacc
     5941 gtctgcgccc ttgattgtga tgaggttctt gccgccctga cctgtaaggt cgaagccttc
     6001 gaagaaggtg tagtcgtcac cgtacagcag ccagtcaaca gcctctgcaa gcggcataag
     6061 aacttcgcaa actgctgcta cgaaggactc tctgtcggtt acgccccaga catggtcttc
     6121 ttcgtatgca tcccatgctg tcaggtcaag ctcaagaact acgcctactg tctcaagaag
     6181 gactctgtcg atttcggctg tgatatcctt gagagcctta agcagtgagt tgactgtgtc
     6241 gtcgttgtag aagtctacct tctcggagag catctctgcg agtgatgcgt agtcgccgct
     6301 tgtcctgatt acggagtcaa cgatttcggc gaggtgagca tcaaggtagt tggctgttgc
     6361 ctttgtccag ttgttcgggt aggtgaggaa gacgattgaa cgctcgggaa gctctatctc
     6421 ggtgtcgccg gtgataggtc cttcgatgtc gtctgtgaac cagagccagt caatctctgc
     6481 catgtcgttt atgcgaccgg caaagagacc ggttactgcg ggcagcaagc cgtcggtctc
     6541 gagcatttcg tcaagaacct ctgcgttgtc gccgtaggtt acaacctcaa ggaggattga
     6601 cagtacgcag gtgaatacat cggcgttgtc aagagctccg taggttgtgt aggctgttgt
     6661 gccgtttgcg gaggtgtagg gaacggcaac attctggaag atgctcttaa ggcctctgcc
     6721 gacaatttcc gcaaactcaa ggtctgcgtc aagctggctt tcaaggaagg ccagcagcca
     6781 atcaagcgaa atggaattca gcagctcgaa ggagaactca aattcgtcct catcttcagg
     6841 ctcctcaggc tctgcggatg cctgggtggt gggctctgtg ggagctgaag ttgtgggagc
     6901 ggttgtctcc gtgggtgctg taggctctgt gggagctgtg gtcggctgtg tggtaggtgc
     6961 tgttgtatct gtcggctcct caggctctgt gggctcttct tcatcctcgt cgtcaccgaa
     7021 gagagactcg gaaagtccgc gcagagtttc tgcaaggctg cggattgtat ctaccgtgaa
     7081 cagaccgttg atagcgttca tgatgtaatc ggagagtgag cctattcctt cgtcttcgtc
     7141 cttaataagg tcgttgacga ttatgtcgat gttgttaagt acatcctgag ccttagcctt
     7201 tgtccagcgt gtgccgtatc cgaggtaaac gatggataca ttgtcgccgt tgccctggtc
     7261 ggtgtacttc cagttcgggg tcttcatgct gtaatcctgc gggttgagaa gctcaactat
     7321 aactgctatt gcagcctgcg gattattggc aagaccgttg attatctcgt ccaggtcgag
     7381 ctcgaagccg tccttaagaa ctgtgtcaag gtcgattgcg tataccggac ggagagtatc
     7441 gatgattgcg taaagcggat agagtatgtt gtttactgtt gtctggatgc cgccgcagtc
     7501 gatgtagtgg agaagcgcgg gcagtatctg gagaatctca ttgatgggat ctgctgcgat
     7561 ttcgttcagt ctgtcgaaca gcggatcgat gatgagtctg agtgctgctg tcgggtctgc
     7621 cgcaactgct gcctcgtact gtgcctgaga tttcaggttc tttacgccca gagcctcaag
     7681 cagcggtact atgccgtatg cgtagccctt gtaaccgtta aggctgatga acgggttgtt
     7741 gttcttgccg actatgaggt tgaggttctg gtcggcaagg aatgccttga gtacgggagt
     7801 caggggagcg agcagtgtgt agagagcgtt catgaagccc tctctgtcgc cgtcctcgaa
     7861 gccccagtca taatcgtcgc cgtactgatc ccatgcgcta aggtcaagac cgaggttctg
     7921 gaggatgatg tcaagaatat caacttcgtc ctcttcctcg tcatcagcgc cgaatgctgt
     7981 aaagcctgct gctgcgaacg cggagaatac ttgtgcgcct gcagcctgct gtactactac
     8041 ttcttcttcc tcgtcgtcat cgctgaatgc tccgagcagt ttctcgcgga caacgggatc
     8101 ggaaatagct tcaaggagat aagagagcag gtagtagaat acatctgcct tgtcggctac
     8161 gatgttgtat ctctgacctg cgggcagacc ggtcatggat acctgcctta cgctgcttgt
     8221 aacctggagc tgtcccatga atgcgagaag acctgtgttg agaagcggga gctgtgcagg
     8281 agcttcttcg tcatcatcgt caaagaggaa gccggagagc agaccgttaa ggcttgtcag
     8341 gtctaaatcc atgtcgtcga ggtcaaggct gtcgcctaca ttgaatgcct ctgtgccgtc
     8401 tgcgagctta atgctaagtg agtgtgactc gatgtagtag taaatggatg tgccaaggtt
     8461 gttaagcaag ggtttgatgc ggtcgaagct gattgcgtat gcgaggttcg gcagcatcga
     8521 aacgattgtg tcaagaggag ccttggcaag cttattctca ataaagtcaa gaataggatt
     8581 gaagattgcg tttacaatgc cttcggttgt ggtgatgttc tgcattgcgg aggcgtttgc
     8641 tataaggctc gggttgcagc cgattgcctc aagaatcgga gcgatggtat tgatgtagcc
     8701 gtggttgccg tatgctgtga ggtcaacatc ggccttaatg gagatgcctg acttttcacc
     8761 ttcggcaacc gagctgctgc ctgctttcca tgtgtagttg cacaggagaa cgcggagcag
     8821 aggttcaata ccctttaagc tctgggcaaa tgcgttcatg aagcgggtct tctttgcatt
     8881 gtagtcctcg agctcgtcaa tgccccagtt gagttccagt tcctcgtctt cattcagaag
     8941 cggtgtccag gagctgttgg aattaagagc gttgtagaca tccgggaaca ttgaagcgtt
     9001 aatcttggat gccagctggt tcgggaatac ctggagaccg aagtcctgag cgattattgc
     9061 gggtgtctta agaatattga tgtcgattgt gactctgaca atgcccttct cgtatctgta
     9121 gctcgacgga atgtctgcta tcatttcatt gagcagttct gcgaatgtgg gatagagagt
     9181 ggacattatc ttgttgaccg actcgtctgt gaacagctct tcagcaagca tgttcttcag
     9241 catatcgtca agcttctcgt cagagcctgt cagcttcagg aagtcctcgc tgaggagaag
     9301 gtcgtctatc ttctttaatg tatcaagcag cctctgttcg gtgactctgt atgcggagtt
     9361 gggatccgag gggtctcttg tcaggtcgcc gcccatgggg ttgcggacat agtaaatgcc
     9421 tgtgccgccg tcaagggtct gctgctgata gatgctgtag ccgccggttg ctacgaagtt
     9481 gttgtactgg ttaaggattg agttgtacag agcggagtac ttgtttctcg tatcctggct
     9541 catgtaagct gtggatgcga ggaagttgta aatgcctgtc tcaactctgc cgatagcatc
     9601 ctttaccatt gtaaagttct ggtatgttat cttgttgtaa gttgagtagt agctgtaagc
     9661 aaggtttact tccgctgtaa ttcttgcctc aagctctcta tacattctgt cgatgtaatc
     9721 attaatctga acgccgaagt tgccgaagat tatatttacg gcatcctgac cgattaatga
     9781 tacagcggtg ttgtagtggt tgttgtaagc tgtacgctgc gattgagctt cgggaataag
     9841 tgttccgtag ataacttctg atttaattgc tgttaagtct gcgccgaaac gcgggataaa
     9901 gaactcatag aactgagcgt aaacggtttc atagttgcgg acattgattt catatctcag
     9961 gtcgctgagc attgatgaaa catatgctgt tccgcttgtg aataccttat ttatattctc
    10021 agcctgatag tttcctaatg tgcttaccca gcctgcgaac tgaccggaag cgacatcaag
    10081 ctgaatgttt gtaagagctg tggaagcgtc gccgaagaag aggtgaggct tgtactgctc
    10141 aagctgatcg gcggggattc tgtactgctc aagctctgcc ttcttttggt cgatagcgtt
    10201 cttgagctgt gtgagctcat agacctcgat atcgtcaagg agctgctctt tgaaggcgtt
    10261 gtactgggtg aggttcattc cgtagttgtt ctgaagaacc gtcttagcct cgccggttac
    10321 agcagccagt gaattataat aggaaactgc tgtggagtac agggacttca tgccgttgta
    10381 atcctgattg ttgtatccgt ttgcgatagc gttctggaac caggtgttcg cgggattcat
    10441 ggcgatgacc gtctgagcgg caacagccct gttcatgaag ttctggatat ccgttaccga
    10501 cggatttgtg aagaagcggt ttctcaggtc ggcggaaata cccatgagta cattgtagta
    10561 atagttgttg ttgttaatca gtgtggcaat tgatgaagcg ttgttgcccg atgccagctg
    10621 atatgtactc tggccaacaa gtgatgtggt gaagtagttg cctgcggcct tgatgtttgc
    10681 ggtgcttgtg ctgtcctgct gagcaacgcc gtaggtaaga ccggagccga agtaatacca
    10741 ctgttttctg tatctcttta tgcccgagtt acctgtcttg ttgccgattg cggttccgca
    10801 gtagtactga tatgcgatat cgattacaga cgggagtgat tcaagtgtgc tgtaactcag
    10861 tattgctgcg ctgacagttc ttgtgacctt tacgccgatg ttgccgtaag agggcaggaa
    10921 cgggtcaggc gtgctgaatg acttttcctt ctcggctctg ttgtttgtgt catatgccga
    10981 tacaacaggg agaagtacat cgatgattgt tgcgccggct ccggaggtat aaccggtaag
    11041 ggacttgatt ctgttacgca taattgtcca ccagttgttg cctgattcgg aggcggtcga
    11101 catttcgtta gcaaaaacag gggccagcgc gacagctata ttgtaaatat aaccaagcgt
    11161 ggtgttgtcg ctaaccgtgt aggtaagatt ctcagcagaa cccgataccg agtagctgcc
    11221 ggtttgtccc gaattgacat aggcggatat ggcgcttgtc agagctgata tctgactgct
    11281 ggacgccgct gccgatgcca tcatcgggaa tgctgcgcta agacatgaca aaaccatgat
    11341 caaagccagc atagaactaa ggattttctt acccatagtt ctaccctttg aagtttccat
    11401 ggacatacat actcctttgc agattttttt gtgccccccc aattaaatgc ggaatatttg
    11461 ggagaggcac aataaatttt ttgaatattt ttttaatatt ccatcaaaat tataaattct
    11521 ctatggaaaa ttgtcaataa actgttaatt aactgcattg tttttattaa attttattag
    11581 gaattcataa ttaagtcaaa tttccgtaca atttatttca aaattgggga agttgcggaa
    11641 tattatgtaa acaaattatt tccacataat gtaaaagtgc cgcaatttta tattaattaa
    11701 aaagttttgt aataatatat ccaaaatatc catattttgc acacaatatc cattattgtt
    11761 aacatagtgt gaaaaaatgg acatagcgca agaaataaaa tcagccgtta agagcaattt
    11821 ctctctaaac ggctgatttt gctgtgtttt tgcctatttt aagctgctgg acgaaagctc
    11881 atgaccttta caaaacccaa attccgacta tttccgtaaa ggcttattgc ttgcctgtaa
    11941 aaaacagcat aaaaacgatg ccataatcgg caattaaacg gaaattttcc ttttcattat
    12001 atattatata gcgtacaagc agaaaaaaaa gccacccgaa agggtggctt agcgtttatc
    12061 ttagatttca gctttccaca gaccgtggag gttgcagtac tcataaaccg caatgggttt
    12121 ttcgtcagca agcgcgaaca ctgccttggg cgcctcgccg ggtttcaggc agatgcgatg
    12181 tccgcccttg tctgtcttga ggtaaatcca cataatgtag tgttcctcaa gcatggggtg
    12241 gtcgacttcg ccaacattaa cggttacttt attgccctca acctttgcta cgggaacatg
    12301 cttttccaaa cttgcgtcaa ctgtatttgc aataagctcg gtcatttcct caccgcagca
    12361 gctaagcgga ccgccgccgt tatgtacaag tcctacaata ttgccgcagt gtttgcatac
    12421 gaaaaacctt acatcacaag ccataaataa acctccctga tttagtttca gtcaaaggtc
    12481 agtgccttag actacggagt tcgctatata gtaacaaact ctattactat tctacatatt
    12541 tcccaaataa attcaagagc aaactttaaa atagactcat gcccaattaa gcccgcctta
    12601 tgataattcc atcgtcagcc tttgcatcaa tgcgggtttc gccgtttctg cggtcaattt
    12661 caaccgtgat tctgccgttt tcggtaagca ttgagccttt cacatagctc agtgacggaa
    12721 tatccgcggg ggctatggtc acatccttaa agcccacagt gccctcagta ttttttattc
    12781 cgagaatata gctgaagaga tgctccacgc tgccgccgaa catgggatgg ctgtgggaat
    12841 gtctgccctc ccattcctcc cagagagtgg tggctccgct ttcgcgcatt ctgtggaagg
    12901 aaaccttgct cttgtttgta agcagcttat atgccagttc tccgtagccg tttttaaaca
    12961 gcacatcgag gagaatatag gtgccgaata tgcccgtgtc gaacctgcct atctgagtgt
    13021 acttatccac aagatttttg agagttctct catctccgag tcccaaatcc gccgcaaagg
    13081 cgtctgctcc ctgaataccc tcaaggaagc tgcccgtttt ttcgtcaaag taattgtcgt
    13141 gcatagcctt tgagcagtcg tcaattacct tattgagata ctcgttgtcc ttgttaatgc
    13201 ctaaaatctt ggcgacctca agggttatgc gggcggtctt aatcaggtag taggtgttta
    13261 caaagggttc gggaatcaaa attatattgt ggggagtgca ccagtcgcca agacaccagc
    13321 cgccctcctc ctctctgacg accagattgt cctcacagcg ggacaccatg tactctatgt
    13381 actttaccat gttggggtaa tactctctga gaacatccgt atccccatag tatcggtagt
    13441 agttgtaggg cacaattacg atagcgccgc cccagcctcc gggaccgccg ccaccgccgt
    13501 agaagggagc cgtatgctga acatggccgc cgaatctgtc ctgattgtcg gcaatatcct
    13561 tcatccactt tttaaacatt tccttggctg aaagggtagt catgcatgct cccgaggcaa
    13621 tctgaccgtc accggtgtag cccagcctct ccctgtgggg gcagtcggag ggaacgcagc
    13681 agtgaatatt gtttagctga gtccttatat aagcctcata gtaccagttg agaaggtcat
    13741 cggagctttc aaactccgag gtattcttga tgtcggtatg aatgaccctg aactccaaag
    13801 gctcggcagt gccgattatc tcaaaatacc tgcccgcgtg ccatgtaaag cggggatagt
    13861 acagactatc gtcttttccg ttgtgaatat aaaaatcctg ctgaatgcgg tgaatgtctc
    13921 ccgcggaaaa gaagtaaaga gtgccgtctt cgtttatttc ctcagcgatt ctgagcagag
    13981 cagtctcatt agcccttgat ttttcgggga acttcagcac gggatagccc gcaacatttt
    14041 cgcccaggtc ataaacgctg tgatctccct ttttgcttat cagctttggc tgtatggttc
    14101 ttattactct gtcaggggga cactcctgct tttccagaat gctcttaggg gcgtccgcca
    14161 aagcgggcgg gtaccactcg gagtcgtcaa agcccgcgtc gttccatcct tcgggctcat
    14221 tgttaagatt ctgaatctcg ccgaagtaga tattcgtcct taaaatatag ctttcataaa
    14281 cttttacagt gctgtcggaa acaattctct ccagttcccc gtcttcatat atgagattta
    14341 tgctgaacgc aagcttcaaa tcgccgcagc gggtaaagcc ttcatttctg ctctcatgct
    14401 gaccgtaaaa tccgttgccc acatgcacgc ccagagcgtt ttccccgtct ttaaggaatg
    14461 aggttatgtc atactcaaga tagtaaaccc tgtgggtaag tgtatcgtaa atcggcatgt
    14521 ggatctttga aagatccctg tattcgtagt ttgtccatgc ggggacattc aggtcatcgg
    14581 taatcctctg accgtttaca taaaactccc caaagccgag ggtgcaaatt ctaagcacag
    14641 cgctcttaac ctttttggta agataaatgg tctttctcgc aaggaacgcc tcggcgttct
    14701 ttctgaacat cagccagcgg cagtctttaa agtcaaggca ttttgccatg tccacatggg
    14761 gtatgtgccc gtccatgtac tcgagtccat agggaacaaa gcccagcttt tcataaaagg
    14821 gcacaaccct tgtctgagct ccgattaaaa gccttgtggc tcccttttcc tcagcctttt
    14881 cagagaggag attgacgagc atgagtccga gacctctgcc tctgtactcc ttgtccacag
    14941 cgattctgcc gagtttataa gctccgccgc caaggtcaat tagccttccg caggcaatct
    15001 ccttgtcctt gtcatagagc acaaggtgtt ccgaaacgct gtccaaagag tcaaactcct
    15061 cctcaaggga atagctttgc tcctcgacaa aaactctttt tctcactttc gcggcgtctg
    15121 aaaaatctcc gcccttttca atccacctta ccttcaaatc cataataaca ccctcaagcc
    15181 cgttgttatt aaaaggaaaa accttcgtta tgtgaagttt aacataccaa caggctcaag
    15241 acaatacgaa aggggcaata aaaactttta tcttgataat attgtggttt atgctatact
    15301 atattttgag tttttgtgta gttaaacgaa aatgaaaagg agtttttctt atggcagagc
    15361 ctaaattggg agtacagctt tatacattaa gggattatat aaagacctat gaggataccg
    15421 acagaacctt tgcctttctt caggaacttg gcgtaaacat cattcagatt tccggcatag
    15481 gcgatttccc cgccatagag caggcggagc tggtccgcaa atacggcatg caggtctgcg
    15541 taacacacaa atcctttgag agaatgcaaa acgaccttga cgctctgatt gacgagcatc
    15601 tgcttttggg ctgcgacagc ataggcgtcg gcagcatgcc cgtatcctac agaggctccc
    15661 ttgaaggcgc aaaggccttt atcaaagagg ccgaaagaat aggcaagcac atgagaaagc
    15721 gcggcgtaag attcaattac cacaaccacg attttgaatt tgaaacctac gaaggccgca
    15781 gaattatgga tgtgctcatt gaggaaagcg accccgaata tgtatggttc gtgccagatg
    15841 tggcttggat tcagattgcg ggcgaaaacc ccgccgaata cattaaaaaa atgcagaacc
    15901 gcattaaggt actgcatttc aaggacttta ttattgatga ggacggcaga aggtttaccg
    15961 aaataggtct cggacagtgc gaccttaaag cctgctacaa aacttgcgta gagctgggcg
    16021 ttccctacat cgtttacgag caggacagcg gctttgccgt aaatcccctt gaatcaacaa
    16081 aggtaagttt cgaaaatatg aagaaaatcg cagcgggcgc tctttaatca gcttgtataa
    16141 tcttcaataa cccgcgcaag gctttcctct ccgtacaccg taatgccgtt ttcaagtatt
    16201 tccgcgtccg ctgggggcaa actaaagaga aatacatcca gtatttgtga aggcacggtt
    16261 tcgtcctcaa caaaaacggc tatcttttct tcataagctc tgacggtgta ctttgtacct
    16321 tgagccgtgg tttccgcgct gtgatattcc gatatatcgc ccccgcccac gcccaaagtc
    16381 tgggttatgg ctatgggaag ggtaagggcg caaagcagca gcgcacccga caccaccagt
    16441 tttcttggtc ttaacatctg acctccccca acacaatttt taaaggcaaa acacgccgca
    16501 aatctatttt gccaacaaat agttgttttt atacaatatc tgctaaaaat aaaaaaagcc
    16561 aacacaatac agcgcacccc gcaagtaaac tccctttacg gcgggaaaca tatgtaaaat
    16621 caaaatcttt acatgtattt tgtgcactca aacctaaagg aggcaacagc cctaatggga
    16681 gaaagcagaa aaaaacaaaa tcccttggct ggtattaaac tcgagagaaa caaaaagcaa
    16741 aaggcaaaaa agcaccactc caaagccttc aacacctttc tcacggttat tttaagctta
    16801 gccattatcg gaatcatatc cgtaacctcg gttgcgatat atgttctgca tgacattgta
    16861 agcatagcaa acggcgatgt ggtcatagat cttgacgagt atatgctcaa tcaaagccag
    16921 accacaattc tttacgccta tgacgaaaac ggcaaagagg ttgaaattgc aaggctccac
    16981 ggcaacgaaa accgcatctg ggtacccctt gaggagatgc ccaaaagcct aattgacgcc
    17041 tatgtcgcca ttgaggacaa gcgtttttgg gaccattcgg gcgttgactg gatgagaacc
    17101 ctctccgtcg tcgtcaaata caggttctcc cagggcggct ccaccatcac tcagcagctt
    17161 attaagaacc tcaccggcga aaacggcaga acctttataa gaaagtacag agagatttgc
    17221 tacgctctca acctcgaaaa gcatgcctct aaggaaaaga tactcgaaac ctaccttaac
    17281 accttatatc ttgacgaggg ctgctacggc gttcagaccg cctccgagtt ctatttcggc
    17341 aaggatgtca gcgagctgaa tctcgccgaa agcgcatgcc ttgccgccat taccaccgct
    17401 cccagaacct ataaccccat ccgcaatttc gagaacaaca gaagaaggca gaagctctgc
    17461 cttgactaca tgctggagca gggcaaaatc tcaaaagaag aatacgacga ggctttaaac
    17521 tacgagatta tctttacgaa cagcgaaaaa tatgtcccca aaaaggacaa caagacaacc
    17581 aaagagaaaa aggacaccta ccagagctac tatgtcgact atgttatcga caaggtaata
    17641 gccgacctcc aggagcagta caactacacc tacaatcagg catggagaaa actctactac
    17701 ggcggactta aaatctacac cgccgaggat atgaatatcc agtccgtttt ggaggacatt
    17761 tactataaca gaaaaacctt gcccaagggc acaaaggacg gtcaggctgt ccagtccgcc
    17821 atgaccgtta tggactatca gggaagactc aagggcatag tgggcagagc cggccccaag
    17881 ccgggcgaca gaagccttaa catcgcggcg aagagcccca gacagccggg ctccgcaatt
    17941 aagcccctca gtgtttacgc ccccgcgata gagaagaact acgcccactg gtcaaccctc
    18001 atgcagaact acggcctagt cttaaaggac ggctctatct ggcccaagaa ctatgacggc
    18061 cccggttcac ccggctcata caagaccata gcggacgcca tacctccttc gctcaacacc
    18121 gtgcccgcaa ggcttgtgga tataatgact cccaaggtat gctacgactt ccttgtgaac
    18181 aacttccaca tttcaacagc cactacttcc gatgtaaacc ttgccccctt ggcggtaggc
    18241 gctatgagca agggcgtcac aacccttgag atggcagccg ctttcgccac cttcggcaac
    18301 ggcggaaagt actacgagcc catctgctat tacagagtaa ccaaccacga cggctcaaag
    18361 gtatatctcc agacacaggc aaagcccgag caggttattt ccgaagccac cgctgacatc
    18421 atgctcaggc tccttgaaag agttatcacc acatccaacg gtacgggccg caagtacgga
    18481 gtttcgggct ttgaaacctt cgctaagaca ggtaccacca ccgacaacaa ggacagatgg
    18541 ttcgtgggcg gaactcccta ttatgtagcc gccgtctggc tcggttatga aacacccaag
    18601 gacctctcac acatctctgg caaccccgcg ggtcaaacct tcaagaccgt tatggacgaa
    18661 atccataagg gacttgaaaa gaaaggcttt acccattccg aggatgttgt caaaaaggtt
    18721 ttctgcacaa gaacgggact tctcgcgagc gaatactgca caagcaagtc cacaggctgg
    18781 tacaagtttg accatgtacc cagcacctgc accacatgca agtattcctc acctcaggta
    18841 aatccgggcg gagtcaccac cgagcctgag gcaggcgagg gaaatgaaga acagccaaac
    18901 cccgagcagt aaattaaatg ccgcctgata tttttcgggc ggcaaatttc agttaaaggt
    18961 gatttaatgg ttattctggc ggttgactac ggagaagccc gcacgggtct tgccgtatgc
    19021 gacaaaaacg aggtgctcgc gacccctctc agccccgtcg tggtcaaaag caccgatatg
    19081 cttatagata cgattataga aaccgcaaag cagtacaaag ccgagctgat tgtggtgggg
    19141 ctccccgtca acatggacgg ctcccacggc ttcagagcgg aggcggtaaa ggagtttgct
    19201 cagcttctga aagaaaagtc gggtatgccc gtagagttct gggacgagcg gcttaccaca
    19261 atgtcggctt caaggcttct taacgaaacc gatgtaaaag gcaaaaaaag aaaaatgaag
    19321 cttgacagcg ccgccgcggc acttattctc cagtctttca tcgactcaag gaaaagaggc
    19381 tgagaggctt ttaatttcgg catgtttaag gaatacaaaa tagccttaat ctattgattt
    19441 aaaggtatat aaaagctaca atataccaaa gatatacagc ctgttttttt ggcttaagaa
    19501 acgagcagtt cgcaaagttt ttctgcggct tgcccgaatt ttgattagga ggtttattat
    19561 ggcgtccttt gacattgacc ccttccgcga caaaaactct gaaaggtata tgctgtccaa
    19621 aagaatcggc aaattggcag aggttctcta caaggattat ttgtacacag ataccgagcc
    19681 ccttggcggc ttttattaca gggacggtca gtattcgccc gctcagataa acgagggcga
    19741 atgggtaccc tttgacagcg aaaacggctg gtggggctat cccgaatgct actgctggtt
    19801 caagcacagc tttaaagtgc ccgaaagatt taagggcaag accgttgttt acaggctcta
    19861 tccttccgac agcggcgaat ggaatgtcaa cccgcagctt ataatcttca tcgacggcaa
    19921 aaccgtacag ggcgccgacc cgaatcacag ggaagtaagg cttcttgaca aggctcaggg
    19981 cggcgaaacc tttaacatac aaatcaacgc gtacacagac ccgttcgcat ggaggggtcc
    20041 cgtcagaatg agggcaaagc tgcacaccct cgatgaggat gtcttaaagc tttattatca
    20101 catcaaaacc cctcttgacg cggcaaacct cttgggcccc gacgatttgg caaggattga
    20161 gatagtaaac aagctcaaca ccgcagtcag catgattgag ttcggcgctc ccgaaagcga
    20221 gtttaaaaag agcgtcctcg aaaccatcga ctaccttgag gaaaaccttt tcggcaaggc
    20281 ccttgacggt gttgaggttt cggcaatcgg acatacccac atagatgttg catggctgtg
    20341 gcgcctgcgt cagacaaggg acaagaccgg caggtccttc gcgaccgtgc ttaagatgat
    20401 ggatgaatat cccgaataca aattcatgtc cccccaggct cagctttacg actttgtaaa
    20461 gcacgactat cccgatgttt atgaaggcat taagcagaga gtcagagagg gccgctggga
    20521 agttgaaggc ggcatgtggg tcgaggcgga caccaatatt t
//
LOCUS       DAONVQ010000017        20176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_1945_length_20176_cov_4.421152, whole genome shotgun sequence.
ACCESSION   DAONVQ010000017 DAONVQ010000000
VERSION     DAONVQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 20176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20176
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1945_length_20176_cov_4.421152"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /gene="mnmE"
                     /locus_tag="PLD98_02185"
     CDS             <1..1183
                     /gene="mnmE"
                     /locus_tag="PLD98_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQA05093.1"
                     /translation="KIDDAVALVFRAPKSYTGENVVEISCHGGVYVLTRALRAALNAG
                     AEPAEPGEFTKRAFLNGKMDLTAAEAVMSVISAHGEQALSAARTALEGALGRKINEIA
                     DSLIKISAGFAVWTDYPEEDIPELDFDAVKGSLEEAKEALSELLSTFEAGQAITEGVR
                     TVICGVPNVGKSSLMNLLCGMERSIVTDIPGTTRDVVEETVKIGNVLLRLADTAGVRD
                     TEDTVENIGVRLAVKRMRDAGLILAVFDGSRELKDEDREFLKALEGKRAIAVINKADL
                     EIKTDIEEIKKSIPELVMLSCKTGKGLDNLRETIEKLLGTENFDTTAPMLANERQRSC
                     CQRALDAIADALEALEMGVTSDAVNVCIDSAINSLLVLTGRKASEAVVDEVFRSFCVG
                     K"
     gene            1202..1702
                     /locus_tag="PLD98_02190"
     CDS             1202..1702
                     /locus_tag="PLD98_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HQA05094.1"
                     /translation="MPKRTDYISWDEYFMGIALLSAQRSKDPHTQVGACIVNQANKIM
                     SLGYNGFPAGCNDDEFPWERTGEDDYETKYPYVCHAELNAILNAGGRDLSGCKLYVAL
                     FPCNECAKAIIQSGIKEVVYLSDKYADTVSVRASKRMFTAAGVKFTKLETKIKEIVIS
                     YREDNI"
     gene            1774..3405
                     /locus_tag="PLD98_02195"
     CDS             1774..3405
                     /locus_tag="PLD98_02195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQA05095.1"
                     /translation="MSDIKQKPVRGSQKMPDHIMLSFSKDPKTSMTVTWRTCTDIKNG
                     YVLYREEGSAEVKRADAVMGLFESDIDISHIFWATPENLKPGTKYYYTCGDDTHRSEE
                     YYFTTEPENLTKFKFICIADHQKASPFDCPDYSELNKFLKDILKQHPDTRFILTGGDN
                     TDCGQHEVQWNGMFSGLTGIIEYVPYMMCIGNHDNRGFKIYREHGSEGRFYSEPAEFF
                     AKQFKGSYPDNGPENWKTENYAFDYGNVHFCVLGVNGPEEVNEWLIDDLNKTDKTWKV
                     GVYHFPICYSGVENENYDAYPQMREGMEMLDILFSGHIHNFCRSFPLKNEELFDRPSQ
                     GTIHYMLGNAHNNPPGSRTSPKIWHAAFYHQEERNQMVTIVEVDGSKMTLTALLNDGR
                     IADQCVIDKEKDLILPYALAPVFNQTRIYYKGMYLGLCSVTVPPVQVDGMWFIAFATL
                     VGYIGGEVKKEKGKVTCSLYGHTATFFEDSDIALTDKGEVKMKAKVFRGHSGQLYVPL
                     DCAAKIFEMRWAYAKRNNFISIEHESDSKTITVQP"
     gene            3613..4305
                     /locus_tag="PLD98_02200"
     CDS             3613..4305
                     /locus_tag="PLD98_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05096.1"
                     /translation="MTYSHEVEKMCKVAKGPHHGPAPIPEEGKWVKAYEIKDISGLSH
                     GVGWCAPQQGTCKLTLNVKDGIIEEALVETVGCSGMTHSAAMAAEILPGKTLLEALNT
                     DLVCDAINVAMREIFLQFAYGRTQSAFSENGLPIGAGLEDLGKGLRSQVGTMYSTKEK
                     GVRYLEMAEGYVTRLALDENDQVIGYEFIKLGTFLEAIRHGVPADEALKKNTGSYGRF
                     NEACRYIDPREE"
     gene            4360..5388
                     /locus_tag="PLD98_02205"
     CDS             4360..5388
                     /locus_tag="PLD98_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HQA05097.1"
                     /translation="MSKAIINDDAISFEGKERRMPRINECLKKYGLSSLTEARELCLS
                     KGIDVEKEVKGVQPIAFENAVWAYTLGVAVAIQSGAKTAAEAASAIGIGLQAFCIPGS
                     VADQRKVGLGHGNLGKMLLSDETKCFAFLAGHESFAAAEGAIGIARSANRVRKEPLRV
                     ILNGLGKDAAMIISRINGFTYVKTEYDYYTDELKIVETIPYSKGERAKVKCYGADDVS
                     EGVAIMRHEKVDISITGNSTNPTRFQHPVAGTYKKWATENGVQYFSVASGGGTGRTLH
                     PDNVAAGPASYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGATVACAV
                     AVEVAMKG"
     gene            5577..6845
                     /locus_tag="PLD98_02210"
     CDS             5577..6845
                     /locus_tag="PLD98_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQA05098.1"
                     /translation="MGVTSEVKKTKYTIVNPYASVDWDTWGVYKANLHTHSTNSDAEI
                     PMADMVEAYYAKGYDILAMTDHGVIGYGWNKPVKTNGIFNFYRPVKTVSEERYKEMRT
                     GADRGGRGMLDIKGGVEVNMAVLAKNHVNGLFTTFGQGILGKENSFREAVEGIHKAGG
                     YSFLNHLGDWTGAKNDREIARNKEYIAYFAEIFRDFRSCLGMEIFNLRDNDTRNDRLF
                     WDELLQVVIPTGRNIWAFANDDAHNLSHVGCSFELFMMPENTVENFKKALVNGTFFAS
                     SRFDKSDPNNIIDDASGLVPVVKRITVNDEEGTISIEPDESRDCKLIEWIANGEVIKT
                     GPSINLNEHEEKLGCYVRFRLVGEGGVTYSQPFELRYEGRVDKKIPSEALDKYRTPMG
                     KVGIAIYRSRPYMISREIVKVIKKKIKKKR"
     gene            complement(7364..9349)
                     /locus_tag="PLD98_02215"
                     /pseudo
     CDS             complement(7364..9349)
                     /locus_tag="PLD98_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
     gene            complement(9417..10811)
                     /locus_tag="PLD98_02220"
     CDS             complement(9417..10811)
                     /locus_tag="PLD98_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQA05099.1"
                     /translation="MTTSEIFLIGLSVLNLIVLILILIKVFSKTSNAEYLTLKQALEH
                     LMRSTADEFQRSRNDNIAFQNIMRESQTALQKQMYDFTNEFTRQISEAQNQFEKNVLV
                     KLGDAEKANLRHYADLSKLLSNSLENIREKNIEQNEKQAKMVEAAILRMQESNEQKLE
                     AMRMTVDEKLSQTLTTRLDSSFKTVSEQLENVYKSLGEMKELSSGVTDNVTALNRVLT
                     NVKARGTWAEIQLESILDQTIPNMYVKNYQSETGKGMVEFAIKIPSGENNSEITYLPV
                     DSKFAMEAYIRLCDAADRADAAALAAARKQLEDAIIKSARDVSKYINPPKTTPFAIMY
                     LATEGLYAEAAASKSGLLERLQREFSVMLAGPSTITALLNSVAMGFKTIAINEKATEI
                     YKILGAAKIQYEKFGDILVKAKKKIEEAGRTLDEAQHRNTIIQKRLSKVEALDVKEAE
                     LLLEEGAEDESATA"
     gene            complement(10953..11630)
                     /locus_tag="PLD98_02225"
     CDS             complement(10953..11630)
                     /locus_tag="PLD98_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HQA05100.1"
                     /translation="MKKAFSFLALFLTVLLITTHGFSALAADAPDTQSAITLTEPRLG
                     IKAKLPNEEAFKVLRLGAYEIVDGVDFEVADNLLGDFVFSLFNIMFIRNQTEVLEQLG
                     SPAQIFLPIPEGYNAQKCAVFRITDGRAVETDFTLNGSELSFTANGSGLFAIVDTSKK
                     PQQVLPGDVNFDGKIRSDDARLVLRAVAKLETLTPFKELAADIDKNGKVNAADARKIL
                     RIYAKLE"
     gene            complement(11650..12666)
                     /gene="galE"
                     /locus_tag="PLD98_02230"
     CDS             complement(11650..12666)
                     /gene="galE"
                     /locus_tag="PLD98_02230"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQA05101.1"
                     /translation="MSVLVTGGTGYIGSHTVVELINAGYDVVIIDNLSNSSEKVLGRI
                     EKITGKTPKFYKCDIRDSKMLDTVFENENIDVVIHFAGLKAVGESVSKPLMYYDNNIG
                     GSVKLFDAMQRHDCKKIVFSSSATVYGSLSKPPFKEDSPVGMTTNPYGTTKLYIEKIL
                     QDLYYSDNEFSISILRYFNPIGAHESGEIGEDPHGIPNNVMPYIAQVATGKLPIFNIF
                     GDKYPTRDGTGVRDYIHVVDLALGHLKAMDRVFSKTGVDIYNLGTGKGYSVLELLTAF
                     EEASGKEIPRRVVGPREGDIAESYADPTKAEKELGWKAERDLKKMCEDTWRWQSKNPN
                     GYEE"
     gene            complement(12685..13515)
                     /locus_tag="PLD98_02235"
     CDS             complement(12685..13515)
                     /locus_tag="PLD98_02235"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HQA05102.1"
                     /translation="MKVGSELVDTKEILWRIVLQEIGGIDGIKPEALKAQVVAAYTYI
                     MYASGKTNPEDNKEYKVTGVAYAKKEKYDEYLKQKENEDSVKNKPDFAVKEAIERAID
                     EVGGKFMTYNGKVINSVFFALSAGKTADSENIWGGNLPYLRSAFSLTDKNEKNYKTEV
                     VLTSAEMRTYLENYIKTLREKYKDNEKYKTFKLSEYEPEKWLSIISHDEAVSESVGYV
                     SDIHINNSVIDPTKPKDYGYICIRGNFFRENVLKDGSKMLLRSPCFTFEYIPKFAPTK
                     "
     gene            complement(13738..13953)
                     /locus_tag="PLD98_02240"
                     /pseudo
     CDS             complement(13738..13953)
                     /locus_tag="PLD98_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
     gene            complement(14043..15290)
                     /locus_tag="PLD98_02245"
     CDS             complement(14043..15290)
                     /locus_tag="PLD98_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05103.1"
                     /translation="MRAALFSIGAQNTGEFKNLKEHIDSVFSAVDQKAALSDYEGLKN
                     AVQAVSAALKECNILVFFLPLKDYFRNKILLFKLFRLEGNVSEDALRKLEALKVYESL
                     SEAEKKAASVFPPNASVIEPVKNEFIAFSVASGKQEIAVIPFADGLTQGLLESYLVSK
                     GLIEAKREEIREIPEDKSQETAPAEETSKAAEEATPAETEREIKFPKLEKYLSENDIK
                     VGVASTPSSIFIKSAGENLNGFDTAFKFIPYIRERSTYDLREYAVNLASLASEMAEAP
                     FGISITNIHTIGEGEDAERFVFIAAAGEKYAKAAKLYALPGEGAEEFLSSAADEALKL
                     LESMIAEYLGDMDYPREAYETPPRPVQFISGAKSIAVIALIAISLIASFLAGFFMLRE
                     KNPDEQIIVSNGIDSYETAEDFV"
     gene            complement(15336..17069)
                     /gene="srtB"
                     /locus_tag="PLD98_02250"
     CDS             complement(15336..17069)
                     /gene="srtB"
                     /locus_tag="PLD98_02250"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR03064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HQA05104.1"
                     /translation="MKSEKVMHNDSLLEQEIENILSSLNLTGGSEGFSSEFTELLERY
                     GVKEDIIRDVQAMSAHAANMPESYTDDNLYSDDLSLYPDVEEPYIPPQPEAYHPGAGR
                     VIYDEDYSSMGNIYMKKTPQTPYYYDENGFRVIYDEDLVNGRGSAPVPERRKPLSAES
                     YIHSKTVKEPDLEGFTEIYKEPDIEVEVSERETEVSGANEKNETPSDLNSLIDPISND
                     WIDYNAKGKKKEKKAHAAKGADSSDNSEKDTESKAVRFFKSFLPWKGDPGKEIARKVI
                     MLASICIIVYYSIYFINYFAQYYGSINDMNDLADLATEYDNSNDTSSVWETIRAKYPN
                     VNFPEKLNPKWANLYAINQDFGGWISIGNTNIDDIVVKSSDNKDYLKTNFYGKENKYG
                     TLFLDYRNFRNGFEELDRNTIIHGHNMKDGLKFAQLEKYMTPEGFAESPIIIYETPYQ
                     KYYWKVYAVIVTNGLPKDDNGYLFNYIVPNFTSDEAFMKYIKALDERKLYDTGVGMLP
                     TDKILTLSTCSYEFKEARLAVIARLVRNGESLDVDTSLVKVNPNPRYPQAWYDAKKLP
                     NPYKDAYKWQP"
     gene            complement(17255..18643)
                     /locus_tag="PLD98_02255"
     CDS             complement(17255..18643)
                     /locus_tag="PLD98_02255"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HQA05105.1"
                     /translation="MDEKINPLENKNINADNKSPSKQLDADFDEALRLLSFDMDSVTE
                     KSSDSSQTADKTADFDAFLESAKSEIEKTAEESKAATQEILNSDAAVDTLPSSPSAAA
                     PPESEPAAASAEESEETDEISKYLDELTPVTDAEGAVVKDNRTIRKERTVFFTFVILT
                     ILMLAFLIFYILIGSSGRYGIGIDIDPDMQNAVFEAPQVFKTLKPNEKEGIQYPEGIQ
                     EKYKRLYAAHQDFVGWLRIPGTPVDTPIYQAKNNTYYLKRDLAGNYSKYGTPFLDYTN
                     RVDKLSRNTVIYGHNFEPKYENDLGFGGIEQYKDVEFYKKHPVIEFNTLYKDYKWKVF
                     ACFLSNGDPSGDNGYLFNYIATSMGNNSFLKFIDEVMQRSFIQTSVDIKETDKILTIS
                     TCAYDFDRGGRLEDLRCVVMARLVREGESEEVDTSKAVQRTDIRYPQLYYDIFGGTNP
                     YKNALKWYPSAD"
     gene            complement(18679..19161)
                     /locus_tag="PLD98_02260"
                     /pseudo
     CDS             complement(18679..19161)
                     /locus_tag="PLD98_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
     gene            complement(19187..>20176)
                     /locus_tag="PLD98_02265"
     CDS             complement(19187..>20176)
                     /locus_tag="PLD98_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQA05106.1"
                     /translation="SYEVLKAMYEHLEAIEGFTFLESTHADPIITEDGQIKGITLTNK
                     KGEKTEVAADFVVAAPGRGGADWLTGIAKLHNIQTRNNEVDIGVRVEVPNSVMDHLTK
                     HLYEAKMVYYSDTFESKVRTFCMNPSGFVSEEHYNAGFNNTFERSIAVVNGHSYADKD
                     KHSGNTNFAMLVSTRFTEPFNQPIEYGRYIAQLGNMLTGGGVMVQRLGDLLLGRRTDA
                     SRLKKSTTVPTLTTAVPGDLSFVLPHRHLTSIIEALRAFDKVAPGLYSKNTLLYGVEV
                     KFYSSKIEVNNKFETSVKNFYAIGDGAGITRGLMQASVSGIVVARDIAEKLKK"
BASE COUNT         4801 a         4791 c         5066 g         5518 t
ORIGIN      
        1 aaaaattgac gacgccgttg cccttgtttt ccgcgcgccc aagagttata cgggcgagaa
       61 tgtggtggaa atctcctgcc acggcggagt ttatgtactg acaagggcat taagggcggc
      121 tttgaacgcc ggagccgaac ccgctgagcc gggagagttt acaaagaggg catttttaaa
      181 cggcaaaatg gaccttaccg ccgcggaggc ggttatgagc gttatatccg cccacggcga
      241 gcaagctttg tccgccgcaa ggacagccct tgagggcgct ttgggcagga aaataaacga
      301 aatcgccgac tccctcataa aaatctccgc aggctttgcc gtctggaccg attatcccga
      361 ggaggatatt cccgagctgg atttcgacgc cgttaaaggc tcccttgagg aggcaaagga
      421 ggctctttcg gagctgctct ccacctttga ggcgggtcag gcgataaccg agggcgtcag
      481 aaccgtaatc tgcggcgttc caaatgtggg aaaatcctct ctcatgaatc ttctttgcgg
      541 catggagcgt tcgattgtta ccgacattcc cggcaccaca agggatgtgg tcgaggagac
      601 cgtcaaaatc ggcaatgtgc ttttgcgcct tgcggatact gcgggcgtga gagataccga
      661 ggacaccgtt gagaatatcg gcgttagact tgccgtaaag cgtatgcgtg acgccgggct
      721 tatccttgcg gtgtttgacg gctcaaggga gcttaaagat gaggacagag agttcttaaa
      781 ggctctggag ggcaaaaggg cgattgcggt tataaacaag gcagaccttg aaataaaaac
      841 cgatattgaa gaaataaaaa agagcattcc cgaacttgtt atgctttcct gcaagacggg
      901 aaagggactg gacaatctta gagaaaccat tgaaaaactg ctgggcaccg aaaactttga
      961 cacaaccgct cccatgctgg cgaacgaaag gcagagaagc tgctgtcaga gggctttgga
     1021 cgcgattgcg gacgctcttg aggctctcga aatgggagtc acaagcgacg cggtgaatgt
     1081 ctgcatagac tctgccataa acagcctgct ggtactcaca ggcagaaagg cgtcggaggc
     1141 tgttgtggac gaggttttca ggagcttttg cgttggaaaa taaatttgtg gggagtttaa
     1201 catgccaaag agaacggatt atatttcctg ggacgaatac tttatgggca tcgcccttct
     1261 ttccgctcaa agaagcaaag acccgcacac tcaggtgggc gcctgcattg taaatcaggc
     1321 aaacaagatt atgtccctcg gctacaacgg ttttcccgca ggctgcaacg acgacgagtt
     1381 cccgtgggag cgtacgggcg aggacgacta cgaaaccaaa tatccttatg tctgccatgc
     1441 ggagcttaac gccatactta acgcaggcgg cagggacctt agcggctgca agctgtatgt
     1501 ggctctgttc ccctgcaacg aatgcgcaaa ggcgataatc caaagcggca tcaaagaggt
     1561 ggtttacctc tcggataagt acgccgacac cgtaagcgtt agagcgtcaa agagaatgtt
     1621 caccgccgcc ggcgtgaaat tcacaaagct cgaaacaaaa ataaaagaaa tcgtaatctc
     1681 ctatcgtgaa gataatatct aacaaagccg ccgcccgaaa aaggcggctg catttatagc
     1741 tttccttgca aaaacaagaa aaggagttct cagatgtcag atatcaagca aaaacccgtc
     1801 aggggctcac agaaaatgcc cgatcatata atgctcagct tttcaaagga cccgaaaacc
     1861 tccatgacgg ttacatggag aacctgcaca gacataaaaa acggctatgt gctttacagg
     1921 gaagaaggct ctgcggaagt taaaagagcc gacgcggtta tggggctttt tgaaagcgac
     1981 attgatataa gccacatttt ctgggctacg cccgaaaact taaagcccgg cacaaagtat
     2041 tactacacct gcggcgatga tacacacagg agcgaggagt attactttac cacagagccc
     2101 gaaaacctta caaagtttaa attcatctgc atcgctgacc atcagaaggc aagccccttt
     2161 gactgccccg attattcgga gctgaataag ttccttaaag atattctgaa gcagcacccc
     2221 gacacccgct ttattctcac aggcggcgac aacaccgact gcggtcagca tgaggttcag
     2281 tggaacggaa tgttcagcgg tcttacgggc attatcgagt atgtccccta catgatgtgt
     2341 ataggcaatc acgacaacag gggctttaaa atttacaggg agcacggttc tgagggcagg
     2401 ttctactctg agcccgcgga gtttttcgca aagcagttta aaggctccta tcccgacaac
     2461 ggccccgaga actggaaaac cgaaaactat gcatttgact acggcaatgt gcatttctgc
     2521 gtcctgggcg taaacggtcc cgaggaggtc aacgagtggc ttatagacga ccttaacaag
     2581 acggacaaga cctggaaagt gggagtatat cacttcccca tctgctactc cggagtggag
     2641 aacgaaaact acgacgccta cccgcagatg cgtgagggta tggaaatgct ggacattctg
     2701 ttcagcggtc atatacataa tttctgcagg agcttcccgc ttaaaaatga agagctgttt
     2761 gacagaccct cacagggcac cattcactat atgctgggca acgctcacaa caacccgccc
     2821 ggctcacgca cctctccaaa gatttggcat gccgccttct atcatcagga agagagaaat
     2881 cagatggtca ccattgttga ggttgacggc agcaaaatga ccctgaccgc ccttttaaac
     2941 gacggcagaa tagccgacca gtgcgttatc gacaaagaga aggacctgat actcccctac
     3001 gctctcgctc ccgttttcaa tcagacgagg atatactaca agggtatgta tcttggtctt
     3061 tgctccgtca cagttcctcc cgttcaggtt gacggaatgt ggtttatcgc tttcgcgacc
     3121 cttgtgggat acatcggcgg cgaggttaaa aaggaaaagg gcaaggtcac ttgcagtctt
     3181 tacgggcata cggcgacctt ctttgaggac agcgacatag cactcaccga caagggcgag
     3241 gtaaaaatga aagcaaaggt tttcagaggt cacagcgggc agctttatgt tcctcttgac
     3301 tgcgcagcta aaatctttga gatgagatgg gcatacgcta agcgcaacaa cttcatatca
     3361 atagagcacg aaagcgactc aaagacaata actgttcagc cgtaattgga attcacgccc
     3421 tcaaaatcgg gggcgttttt aatttatccg caaagaccgt tagttttttg acaatttgta
     3481 tgaaatatcc ttacttacac tttcaaagct atatattttt ttacttgcgg attttgtcgc
     3541 atttagatat aatatttttg tttgctgcat gaagcatttt ccaattgtaa attcgaaagg
     3601 agaagtacaa gaatgaccta ttcacatgag gttgaaaaaa tgtgcaaggt tgcaaaaggc
     3661 ccgcatcacg gtcccgcgcc gattcccgaa gagggcaaat gggttaaggc atatgagata
     3721 aaagacattt cgggcctttc ccacggcgtc ggctggtgcg ctccccagca gggcacctgc
     3781 aagcttacac ttaatgttaa agacggaatt attgaggaag cccttgttga aacggtaggc
     3841 tgctcgggca tgacccattc ggcagccatg gcagcggaga ttcttcccgg caagactctg
     3901 cttgaggctc ttaacacaga ccttgtttgc gacgctataa atgttgcaat gagggagata
     3961 ttccttcagt ttgcgtacgg ccgcacccag tccgcattct cagagaacgg acttcccatc
     4021 ggcgcaggtc ttgaggacct gggcaaggga cttcgctcac aggtcggcac aatgtactca
     4081 accaaagaaa agggcgttcg ctatcttgag atggcagagg gctatgtaac ccgccttgcc
     4141 cttgatgaga acgaccaggt tatcggctat gagtttatta aactcggcac attccttgag
     4201 gctattcgtc acggcgttcc cgctgacgag gctctcaaaa agaatacggg cagctacggc
     4261 cgctttaacg aagcatgccg ctacatagac ccgagggaag aataaggagg agcgaatatg
     4321 acagagaata aattcagtgg gccttcatgc actcccagta tgagcaaggc tatcataaat
     4381 gacgacgcca tctcctttga gggcaaggaa aggcgcatgc ccagaatcaa cgagtgcctt
     4441 aaaaagtacg gtctttcaag ccttacagag gcaagagagc tttgcctttc aaagggcatt
     4501 gatgttgaaa aggaagtaaa gggcgttcag cccatcgcat ttgaaaacgc tgtttgggca
     4561 tatacactcg gcgttgccgt cgctattcag tccggcgcaa agaccgccgc tgaggccgca
     4621 tccgctatcg gaatcggact tcaggcattc tgcatccccg gctccgttgc cgaccagaga
     4681 aaggttggtc tcggtcacgg caatctcggc aagatgctgc tttctgacga aaccaagtgc
     4741 ttcgctttcc ttgcaggtca cgagtccttt gcggctgcgg aaggcgcaat cggaatcgcc
     4801 cgttccgcta acagagtccg caaggagcct ttgagggtta tccttaacgg tctgggcaag
     4861 gacgcagcta tgattatctc cagaatcaac ggctttacct atgtaaagac cgaatatgat
     4921 tattataccg acgagctgaa gatagttgaa actatcccct actccaaggg cgagagagca
     4981 aaggtaaaat gctacggcgc ggacgatgtc tccgagggcg tcgctatcat gcgccacgag
     5041 aaggttgaca tctccatcac gggcaactcc accaacccga cccgcttcca gcaccccgtc
     5101 gccggtacat acaagaagtg ggctacagaa aacggcgtac agtacttctc cgttgcgtcg
     5161 ggcggcggca caggcagaac gctgcatccc gacaatgtgg ccgcaggccc cgcttcctac
     5221 ggaatgaccg acaccatggg cagaatgcac agcgacgctc agttcgcagg ctcctcatcg
     5281 gttcccgctc atgttgaaat gatgggactt ataggcatgg gcaacaaccc gatggtcggc
     5341 gctaccgttg cttgcgctgt tgctgttgaa gttgcgatga aaggttaaaa tatcaaggct
     5401 tccgacctta gtcgggagcc tttttgtatt ttaaagagga aataagcttt ttgtctatta
     5461 gctatgcaaa tattgctctt aataaatgag cttatattat tgacctcaga gctcttttag
     5521 ggtattttga aataaatatc gatacaatct caatatttgt gactggagcg atttgtttgg
     5581 gtgttacatc tgaggtaaag aaaacaaaat acaccatagt taatccatac gcgagcgtcg
     5641 actgggacac atggggcgtt tacaaggcta accttcatac ccactccaca aacagcgacg
     5701 cggaaattcc catggcggac atggttgagg cttattacgc taagggctac gacattctcg
     5761 ccatgaccga ccacggagtt ataggctacg gctggaataa gcctgttaag acaaacggca
     5821 ttttcaactt ctaccgccct gtaaaaaccg tcagcgagga aaggtacaaa gaaatgagaa
     5881 ccggcgccga caggggcggc aggggtatgc ttgacataaa gggcggcgtt gaggtaaata
     5941 tggctgtcct tgcaaagaac catgtaaacg gtctgttcac cacattcggt cagggcattt
     6001 taggaaaaga gaacagcttc agagaggctg ttgaaggcat acacaaggca ggaggctact
     6061 cgttcttaaa tcacctcggc gactggacgg gcgctaagaa cgacagagag atagcaagaa
     6121 ataaggaata catagcctac ttcgcggaga ttttcaggga cttcagaagc tgcctcggca
     6181 tggagatatt taatctcagg gacaacgaca cccgcaacga cagactcttt tgggatgagc
     6241 ttttgcaggt ggtcatccct acgggcagga acatctgggc attcgctaat gacgacgccc
     6301 acaatctctc ccatgtgggc tgctcctttg agctgttcat gatgcccgaa aacaccgtgg
     6361 agaactttaa gaaagctctg gttaacggca ccttctttgc ttcaagccgc tttgacaagt
     6421 ccgacccgaa caatataatt gacgacgcca gcggtcttgt gcccgttgta aagcggatta
     6481 ccgtaaatga tgaggaaggc acaatctcca tagagccgga tgaaagccgc gactgcaaac
     6541 tgattgagtg gattgcgaac ggcgaggtta ttaagacggg cccctctata aacctaaatg
     6601 aacatgaaga aaaattaggc tgctatgtac gcttccgcct tgtgggtgag ggcggcgtta
     6661 cctactccca gccctttgag ctgagatacg agggcagggt tgacaagaag attccctcag
     6721 aggctctgga taagtacaga acgcccatgg gcaaggtcgg catagcgatt taccgctcaa
     6781 ggccctatat gatttcaaga gaaatcgtca aagtaataaa aaagaaaata aagaaaaaga
     6841 ggtaacggca cggccgctcc cctttttaag acaaattaag gaggccttta ggcctccttt
     6901 tttgtattta tattgaattc atcagctttc cgacattctt tatggctgtg gtaagtgaga
     6961 gtaccatttt gcctattccc ttgggaacgc tgccgccgtt gagaatgtct atgaaagcgt
     7021 ccgccatatc ctgagtaaag acgcccatgc tcattgacac aaagcagcgg atgggtatct
     7081 gcatagccat tgccttcatc atattctcgt tggcgctgtc ctcgcccatt gccgaaaggg
     7141 ctttgcttat aatgttgttg attaccctgc ccgcctttgt ggtgttggcg tcctcaaggg
     7201 tggagttaag gtcgatgggc tttccttcgg ggcgagtgga ctcgggtatg ggtctgccga
     7261 gcagagcctc aaactcgctg tcgggaacgg agagaacatt cgcgccgtag taggagggtg
     7321 ctttgtcttt gagattaagc tgaggtcttt cctctgtttc tgtaagctgt accgcagctt
     7381 tgagcctgat gtcacggctg gatgcgccga caaggatatt gtacttgcct gtgggggcat
     7441 gccagtcgcg gagctcaaca ttgtaatagg caaatgagcg cttatcgagc tcgatttcaa
     7501 ccgtcttttc ttcgccgggc tccaggaaca cctttttaaa gcctttaagc tccttttcgg
     7561 ggcggaatac gacggaatcc tccgcgctta cataaacctg agcgatttca gctcccgcca
     7621 tatcgccgat attctttatc ttgaaggata cggtcaatgt gtcggtgtcc ttaatcttct
     7681 tggcggagag ttttatgttt gaatactcga attcggtata tgacaaaccg aaaccgaagg
     7741 ggaagagaac atcctttttg gcggtgtcga aatagcggta gccgacatat atgctctccc
     7801 tgtactcaac cgtgcagtgg gcgccgggga agttcgcggt gctgggaatg tcgtcaagac
     7861 tgtaggcata ggtctcagcc agcttgcctg aggggtttac atcgcccaca agaatgtccg
     7921 cagcagcctt gccgcctgcc tgacccgcaa gatatgtgtg cagaacagcc tttacctttg
     7981 aaagccacgg cattgtgact gccgagccgc cggcaaggac gacgatgaca ttgtcgtttt
     8041 cctctgcgac cgcctcaatc agctcgttgt ggctgttggg caggtcgata tgggtgcggt
     8101 catagccctc ggagtcgaag gcgtcggtaa gtcccgcgaa gataagaacc gcgtcactgt
     8161 tcttggcgca ttttaacgct tcctcaatca gcttttcgtt gacttcatcc ttggacttgt
     8221 catatccggg ggcgtatttt acattcagac ccttttcgat aagcgcgtca aaggcgttgt
     8281 caagccttgt gggattgata agcgagctgc ctgcgccctg atatctgggc gagcgagcca
     8341 tttcgccgat gacagctacc gtcatgccct ttttgagcgg gaggatttta tcctcgtttt
     8401 tgagaagaac catggactgg cttgctatct ttcttgcaag ctcgtgatgt gtcggctcgg
     8461 aatagcttgt gttctggagg tgtttgcttg ctctgaatat aacatgaagg attctgttga
     8521 ctgctctgtc aagaactgcc tcgtcaagct ctccgttttg gactgcctcg actatcttct
     8581 tggcgccgag tccgcttgaa gtgggcattt caagctcctg accagcttta aggccttcta
     8641 ccctgtcatt ctctgcgccc cagtcggtga cgacaaagcc cttaaagccc cactcgtttc
     8701 ttaaaacatc cgtaagcagc cacttgttct cgctgcagta ggtgccgttg agcttgttgt
     8761 aggcgttcat tacggtccag ggctggctct tttttacagc ctcctcaaag cccgcaaggt
     8821 agatttctct cagcgcgcgc tcgtcaacaa ccgaatcgat ggtcattctt cttgtttcct
     8881 gattgttggc ggcaaaatgc tttaaggatg tacccacacc ctttgactgg acgccttcga
     8941 ttaaggctgc tgccagcttg cccgtaagga aggggtcctc cgagaaatac tcaaagtttc
     9001 tgccgcataa gggggaacgc ttgatgttga cgccggggcc cagaagaatg gatactcttt
     9061 cggcaaggca ttcctcgccc agagcctcgc ccatttccct taaaaggtcg gggtcccatg
     9121 agcatgccgt aaggcatgcg gtggggaagc aaacggcggg aacggagctt aaaagctcgc
     9181 ccttgccctt tttatccttc ttttttctca gtccgtgagg tccgtcggta accatgatgg
     9241 acgggatgcc gagtctttcg tttccggcaa ggtgccagaa atctatgcct gagcagaatt
     9301 cagccttttc ttggagcgtc attttgccaa tcagttcagc gtacttcatg ttattccctc
     9361 cagtttatgt tttgaaacct taaatatggg tattgtatcc agatttaaca agcggtttaa
     9421 gcggtggcgc tttcgtcctc tgcgccttct tcaaggagaa gctctgcctc tttgacatcc
     9481 aaagcctcaa ctttggagag ccttttttga attatggtgt tgcggtgctg agcctcgtca
     9541 agggttctgc cggcctcctc aattttcttc tttgccttaa cgaggatgtc gccgaatttt
     9601 tcgtactgaa tcttcgccgc gccgagaatc ttataaatct cggttgcctt ctcattaatg
     9661 gcgattgtct taaagcccat ggcgacggag ttaagaaggg cggtgattgt tgaggggcct
     9721 gccagcatga cgctgaattc cctttgcagc ctttcaagca gacctgactt tgaggcggct
     9781 gcttccgcgt aaagtccctc tgtggcgagg tacattatcg caaacggggt ggttttgggg
     9841 ggattaatgt attttgatac atccctcgcg gactttataa tagcgtcttc gagctgtttg
     9901 cgggctgcgg cgagggctgc ggcgtcggct ctgtctgccg catcgcagag ccttatatat
     9961 gcctccatgg cgaacttgga gtcgacgggg aggtaggtaa tctccgaatt attctcgccc
    10021 gacgggattt ttatcgcgaa ctcaaccatt cccttgcccg tttcggactg atagttcttg
    10081 acatacatat tgggaatggt ctggtcaagg atggactcaa gctgaatctc cgcccatgta
    10141 ccccttgcct tgacattggt gagcactctg ttcagggcgg tgacattgtc ggtaacgccg
    10201 cttgaaagct ccttcatctc gccaagggac ttatacacat tttcaagctg ctcggaaacg
    10261 gtcttgaatg aggagtcgag ccttgtggta agagtttggc taagcttttc gtccactgtc
    10321 attctcattg cctcgagctt ttgctcgttg ctctcctgca ttctgagaat ggcggcttcc
    10381 accatctttg cctgcttttc gttctgctct atgttctttt ctctgatatt ctcaagggaa
    10441 ttggaaagga gttttgaaag gtcggcgtag tgcctgagat ttgccttttc cgcgtcgccc
    10501 agctttacaa gaacattttt ttcaaactgg ttctgggctt cgcttatctg acgggtgaac
    10561 tcgtttgtaa agtcgtacat ctgcttttga agggctgtct gggactccct cattatgttc
    10621 tggaaagcta tattgtcgtt gcggctgcgc tggaattcgt ccgcggtgct cctcattaaa
    10681 tgctccaaag cctgctttaa tgtgagatac tccgcgttgg atgtttttga aaagactttg
    10741 ataaggatta agattaacac tatgagattt aaaacgctaa gacctattaa gaatatttct
    10801 gatgttgtca tatttaccct ttcattattt tttataaaaa atagtccttt gtaaaaattt
    10861 atcttattta ttataccaaa atctaaccta attgttaaca gtattttgga agaagtgctt
    10921 taaataaaaa aaccgtctaa gacggtcttt ttttactcaa gctttgcgta gattcttagt
    10981 atcttccttg cgtcggcggc atttaccttg ccgtttttgt caatatccgc cgcgagctcc
    11041 ttgaaggggg tcagcgtttc cagctttgcc accgctctta aaacaagtct tgcgtcgtcg
    11101 gacctgattt tgccgtcgaa attgacatct cccggaagta cctgctgcgg ctttttgctt
    11161 gtatcaacta tggcgaaaag gccgctgccg tttgcagtga aacttagctc agagccgttt
    11221 aaggtaaaat cggtttcgac cgccctgccg tcggttatcc taaataccgc gcacttttgg
    11281 gcattatagc cttcgggaat gggcaaaaag atttgagcgg ggctgccgag ctgctctaaa
    11341 acttcggttt gattgcggat aaacattatg ttaaagagtg agaacacaaa atcgcccaag
    11401 agattgtcgg cgacttcaaa gtccacacca tcgacaatct catatgctcc aaggcgcaaa
    11461 accttaaaag cctcctcgtt cgggagctta gccttaatcc ccaaacgggg ttctgtgagt
    11521 gtgattgcgg actgagtatc cggagcgtct gccgctaagg cagaaaaacc gtgggtggtg
    11581 ataagaagaa cagtaagaaa aagcgccagg aaagaaaatg cctttttcat gttctcaccc
    11641 gtcttttatt tactcctcat agccattggg attcttgctc tgccatctcc aagtgtcttc
    11701 gcacatcttt ttaaggtctc tttcggcttt ccagcccaac tctttttccg cttttgtcgg
    11761 gtctgcgtag gattcggcaa tatcaccctc gcggggtccc acgactctgc ggggaatttc
    11821 tttgccgctt gcctcctcaa atgcggttaa aagttctaag accgaatagc cttttcctgt
    11881 gccgaggttg taaatatcaa cgcctgtttt ggagaatacc ctgtccatag ccttgaggtg
    11941 accgagggcg aggtcgacaa catggatata gtccctgact cccgtgccgt cccttgtggg
    12001 gtacttgtcg ccgaaaatat tgaaaatcgg cagcttgccc gttgcgacct gcgctatata
    12061 gggcattaca ttgttgggga taccgtgggg gtcctcgccg atttctccgc tctcatgggc
    12121 gccgatgggg ttaaagtatc tgagaatgga tattgaaaac tcgttgtctg agtaataaag
    12181 gtcctgaagg attttctcga tatacagctt agttgtgccg taggggtttg tggtcatgcc
    12241 cacgggggaa tcctctttaa aaggaggttt gctgaggctt ccgtaaaccg ttgcggagga
    12301 gctgaaaact atttttttgc agtcgtgcct ttgcatcgcg tcaaagagct ttacgctgcc
    12361 gcctatatta ttgtcgtagt acataagcgg ctttgaaaca gactccccaa cagcttttaa
    12421 gcccgcaaag tggattacca catctatgtt ttcgttttcg aaaacggtat ccagcatctt
    12481 tgaatctctg atgtcgcact tatagaactt tggggtttta cccgtaatct tttcgattct
    12541 gccgaggacc ttctcggagc tgttggaaag attgtctata attacaacat catagcctgc
    12601 gtttatcagc tcgacaacgg tatgggagcc gatataacct gtgccgccgg taacaagaac
    12661 tgacactggc cttcctcctt attgttattt tgtaggcgcg aatttgggta tgtactcaaa
    12721 ggtaaagcac ggtgagcgaa ggagcatttt gctgccgtcc tttaaaacat tctctctgaa
    12781 gaaattgccc cttatacata tgtagccgta gtccttgggt ttagtggggt cgattacgga
    12841 attgtttata tgtatgtcgg acacatagcc aacagattcg cttaccgctt cgtcatgcga
    12901 gataatagag agccattttt ccggctcata ttctgagagc ttgaatgttt tatatttttc
    12961 gttgtccttg tatttttctc tgagggtttt tatataattt tcaaggtatg tacgcatctc
    13021 agcggaggta aggacaacct cggttttgta gtttttctca tttttgtccg taagactaaa
    13081 agcagacctt aagtacggca aattgccgcc ccaaatgttc tcggaatccg cggttttgcc
    13141 tgctgacagc gcgaagaata cggagtttat aaccttgccg ttataggtca taaacttgcc
    13201 gccgacttcg tctattgccc gctctatggc ttctttaacg gcaaagtcgg gcttgttttt
    13261 taccgaatcc tcgttctctt tttgctttag gtattcgtcg tacttttctt ttttggcgta
    13321 tgctacgccc gtcaccttat actctttatt gtcctcggga tttgtcttgc ccgacgcata
    13381 catgatatat gtatacgcag cgactacttg ggctttaagc gcctctggct ttatgccgtc
    13441 aatgccgcca atctcctgca ggactatgcg ccaaagaatt tctttggtgt ctaccagctc
    13501 ggagcctact ttcatttttt cgggcatctc ttcattggca aactctatgc cattatagta
    13561 atgggagagg atttcctcgt atttataata gctgccgtct tccttttggg tgtttgccat
    13621 acgagctgcg ccgtactggc tcatgccaac gccgtgaccc cagccgtaaa cggtaaatac
    13681 aaagtcaccc attacggggt actcaagctt tgcggctatt ctgaggacga ttctcgcgtc
    13741 cgcggcgttg accgtgccgt caaaattcgc gtcggcgtac tttagattct cgcctttaag
    13801 ctcgctgagt ttggaaaaat gacggagaat cagccttgcg tcgctggcgt cgattttgcc
    13861 gtcgagattc gcgttgccgc ctgcgtagac aattttgggc tcggcgtttg cggcaaaggc
    13921 tgcgggagaa agaacgccca caaataccgc catgcagata agaaggcaga aatatttatg
    13981 tattctgttt gttttattca ttctctcaca acccttgcaa aaaacggaag ggattttgtc
    14041 ttttaaacaa aatcctccgc cgtttcataa gaatctattc cgtttgatac tattatttgt
    14101 tcatcgggat ttttctccct gagcataaag aagcccgcaa ggaaagaggc tataagggag
    14161 attgcaatca gggcgattac ggctatgctc tttgccccgg aaataaactg aacgggtctg
    14221 ggcggggttt cgtaagcctc tctgggataa tccatatcgc caaggtactc cgctatcatg
    14281 ctttcaagaa gcttcaatgc ctcgtccgcg gctgaggata aaaactcctc cgctccctcg
    14341 ccgggcaagg cgtagagctt tgccgccttt gcgtattttt cgcccgcggc ggctataaag
    14401 acaaagcgtt cggcgtcttc gccctctcct atcgtgtgaa tgttggtgat ggagatgccg
    14461 aagggggcct ctgccatttc ggatgcgagg ctggcaaggt ttacggcgta ttccctaagg
    14521 tcataggtgc tgcgctctct gatataggga atgaacttaa aggctgtgtc aaagccattc
    14581 aaattttcgc ccgcggattt gatgaatatt gatgagggcg tgcttgcgac gcctacctta
    14641 atatcgtttt cggaaaggta tttttcaagt ttcgggaact ttatttcccg ctctgtttcg
    14701 gcgggagttg cctcttcggc tgcctttgag gtttcctctg cgggagctgt ttcctgagac
    14761 ttgtcctcgg ggatttctct gatttcttcc ctttttgcct caattaagcc ctttgaaaca
    14821 agataagact ccaataaacc ttgtgtaagt ccatccgcaa aggggataac cgctatctcc
    14881 tgcttgccgg aggcaacgga gaaggctata aattcatttt taacaggttc gattacggac
    14941 gcattcggcg ggaaaacact tgccgccttt ttttccgcct ccgaaaggct ttcataaacc
    15001 tttagagcct caagctttct gagcgcatct tccgaaacat taccttcaag gcggaagagc
    15061 ttaaacagca ggattttgtt tctgaaataa tcctttaatg gcaggaagaa tacaagaata
    15121 ttgcattctt taagcgccgc ggaaacagct tgtacggcat ttttaagacc ttcatagtcc
    15181 gaaagggctg ccttttggtc gactgccgaa aatacggagt caatatgctc cttaagattc
    15241 ttaaactctc ctgtattctg agcccctatg gaaaatagcg ctgctctcat caaataacgc
    15301 gtcctttcgt caagagaaaa caattggttc ggctgttacg gctgccactt gtatgcgtct
    15361 ttgtaggggt tgggcagctt tttggcgtcg taccatgcct gaggatagcg aggattggga
    15421 tttaccttga caagggaggt gtccacatcc aggctctcgc cgtttctgac aagccttgct
    15481 ataacggcaa gccttgcttc cttaaactca tagctgcagg ttgagagggt gagaatcttg
    15541 tccgttggga gcatgcccac tcctgtgtcg tagagctttc tctcgtcaag agccttaata
    15601 tatttcataa atgcttcgtc gcttgtaaaa ttaggaacga tgtagttgaa cagatagccg
    15661 ttgtcgtctt ttggcagacc gtttgtaacg ataacggcat ataccttcca atagtatttc
    15721 tgataaggtg tttcgtaaat gataatcggc gactcggcaa agccctcggg tgtcatatac
    15781 ttttcaagct gggcgaactt gagtccgtcc ttcatgttgt gaccgtggat tattgtgttg
    15841 cggtcaagct cttcaaagcc gttcctgaag tttctgtagt caaggaacag tgtgccgtat
    15901 ttattctcct tgccgtagaa gttggttttg aggtagtcct tgttgtcgga gctcttaacc
    15961 acaatatcgt ctatgtttgt gttgccgatt gaaatccagc cgccgaagtc ctgattgatg
    16021 gcgtagagat ttgcccactt ggggttaagc ttttcgggga agtttacatt cgggtatttt
    16081 gcccttatgg tttcccaaac ggaagaagtg tcgtttgagt tgtcatattc ggtggcaagg
    16141 tcggcaaggt cgttcatatc gtttatgctg ccgtagtact gggcgaaata gttgataaag
    16201 taaatgctgt aatagacgat tatgcagatg gatgccagca taatcacctt gcgggcgatt
    16261 tccttgcccg ggtcgccctt ccaaggtaaa aagctcttaa agaaccttac cgcttttgat
    16321 tctgtatctt tttctgagtt atcagaagaa tcggcgccct ttgcggcgtg tgctttcttc
    16381 tccttctttt tgcccttggc gttatagtct atccaatcgt ttgaaatagg gtcgataagg
    16441 gaattaaggt cggaaggagt ttcgtttttt tcgtttgcgc cgctgacttc agtttctctt
    16501 tcagatactt ctacttctat atcgggttct ttataaatct cggtaaatcc ctcaaggtcg
    16561 ggttctttaa ccgtttttga atgaatgtag ctttctgcgc tgaggggctt tcttctttcg
    16621 ggaactgggg cgctgccgcg gccgtttacc aggtcctcgt cgtaaataac tctgaagccg
    16681 ttttcgtcgt agtaataggg agtctgagga gttttcttca tgtagatatt gcccattgaa
    16741 gaataatcct cgtcatatat gaccctgccc gcgccggggt ggtatgcttc gggctggggc
    16801 gggatatagg gctcctcaac atcgggataa agtgagaggt cgtcggagta aagattgtca
    16861 tcagtgtaag attcgggcat attcgcggcg tgagcgctca ttgcctgaac atcgcgaatt
    16921 atatcttctt taacaccgta tcgctcaagc agctcagtga attcggacga aaaaccttca
    16981 gaccctcccg ttaaattgag agagctgagg atattttcta tctcctgctc aagcaggctg
    17041 tcgttgtgca ttactttttc gcttttcatt tccgacaacg aaatcacttc tttcatcaac
    17101 cgaaaacggc gctttcagtt tcattaaacc gcttaaaaac ggctgcgaac tttgtgtaaa
    17161 ttatattata cctttaattt cgatcgcttg caattatatt tttggttgtt ttgctcaaaa
    17221 attgtggcaa attctctaaa ctttgcgaaa aaactcagtc tgcggagggg taccacttga
    17281 gagcgttttt gtaaggattg gttccgccga aaatatcgta gtaaagctgg ggatatctaa
    17341 tatcggttcg ctgaaccgct ttggaggtgt caacctcctc gctctcgcct tctctgacta
    17401 atcttgccat gacgacgcac cttaaatcct caagtctgcc accgcggtca aagtcgtagg
    17461 cgcaggttga gattgtcagg attttatccg tttctttaat gtccacgctt gtctggataa
    17521 agctcctttg cataacctcg tcaatgaact tcaaaaagct attattgccc attgaggtgg
    17581 caatataatt gaaaaggtag ccattgtcgc cgctggggtc gccgtttgag aggaagcagg
    17641 cgaaaacctt ccatttatag tccttgtaaa gtgtgttgaa ctcgataacg gggtgctttt
    17701 tgtagaactc cacatccttg tactgctcaa tgccgccaaa gcctaagtcg ttttcgtact
    17761 tcggctcaaa attgtgaccg tagatgacgg tattcctgct gagcttgtca actctgtttg
    17821 tgtaatcaag gaagggggtt ccgtactttg agtaattgcc cgcaaggtcg cgctttaggt
    17881 agtaggtgtt gttttttgcc tgataaatgg gagtatccac cggggtgccg gggattctga
    17941 gccagccgac aaaatcctga tgagcggcgt aaaggcgctt atacttttcc tgtatgccct
    18001 cggggtactg aataccctct ttttcgttgg gctttaatgt cttgaatacc tgcggggcct
    18061 cgaaaacagc gttctgcata tcggggtcga tatctattcc tatgccgtat ctgccgctgc
    18121 ttccgataag tatatagaaa ataaggaacg ccagcataag tatggttagg ataacaaagg
    18181 taaagaatac agtgcgctct tttcttatgg ttctgttgtc cttgacgacc gctccctcag
    18241 cgtccgttac gggagtaagc tcgtcaaggt attttgatat ttcgtctgtt tcttcacttt
    18301 cttctgccga tgccgcggcc ggttcggact ctgggggggc tgctgccgac ggggaagacg
    18361 ggagtgtgtc gactgcagcg tcggagttta gaatctcctg agtggcagcc ttgctctcct
    18421 cggctgtttt ttctatttcg cttttggcgc tctctaaaaa ggcgtcaaaa tcggccgttt
    18481 tgtcagcagt ctgagatgaa tcggagcttt tttcggtcac agagtccata tcaaagctca
    18541 aaagacgcaa agcctcgtca aaatcagcat caagctgttt tgaggggctc ttattgtctg
    18601 cgtttatgtt tttattctca aggggattta tcttttcgtc cataaacttc accatgaaaa
    18661 ggagttacgg tgatggggtt aaagctgctc gaggaactcc gagagcattt tcagagcctc
    18721 gttggcggca aagtttctgt tctcggttct aacatcgccg ctgaaggagg ttctgaattc
    18781 ctttgtgacc gaatagcctt cggctgaaag ggaaatgtaa atcagaccta cgggctttga
    18841 tgtattgtcg ctgccgggac ccgcgattcc cgtaatgccg atgccgatat ccgtactgcc
    18901 ggctttcctt acgccttcgg ccatttcctc ggcggtctcg cggctgacag caccgtgggc
    18961 agataaggtt tctggcttta cgcctaaaaa ctgcagcttt gcgtcgtttg aataggcgac
    19021 gattccgtac ttatagaagg ctgatgagcc ggggatgtcg gttatcagct ttgaaacaag
    19081 accgcctgtg catgactcgg ctaaacttag cgtcaagccg aggcttgtca gacgctgcgc
    19141 tataaccctt aagtctacag tcatgctctt acctgccctt ttggtgttat ttcttaagtt
    19201 tttcggcaat atctcttgcc acaactatgc cgctgacgga tgcctgcata agtcctctgg
    19261 ttatgcccgc gccgtcgccg atggcataga aattcttaac agaggtttca aatttattgt
    19321 ttacctcaat ctttgaggag tagaacttaa cctcaacgcc gtaaagcagg gtattcttgg
    19381 agtacagtcc tggagcgacc ttgtcgaatg cccttaatgc ctcaataatg ctggtgaggt
    19441 gtctgtgggg cagaacgaat gaaaggtcgc cgggcacggc tgtggtgaga gtgggaacag
    19501 ttgtggactt tttaagtctt gaagcgtcgg ttcttctgcc gaggagcaaa tctccgaggc
    19561 gctgaaccat tacgccgccg ccggtgagca tattgcccaa ctgggcgata tatctgccgt
    19621 attcaatcgg ctgattgaag ggctctgtaa accttgtgga aacaagcata gcgaagtttg
    19681 tgttgccgct gtgcttgtcc ttgtcagcgt agctgtggcc gttaacgacg gctatgctgc
    19741 gctcaaaggt attgttaaag cccgcgttgt aatgctcctc gctgacaaag ccgctggggt
    19801 tcatgcagaa ggtgcgtacc ttgctctcga atgtatcgga gtaataaacc atcttagcct
    19861 cataaaggtg ctttgtaaga tgatccatga cggaattggg gacctcgact ctgactccga
    19921 tgtccacctc gttattcctg gtctggatat tgtggagctt tgcgatgcct gtaagccaat
    19981 cggcgccgcc tctgccggga gcagcgacaa caaagtccgc ggcaacctcg gtcttttcgc
    20041 cctttttgtt tgtaagagtg attcctttaa tctgaccgtc ctcggtgata atcgggtccg
    20101 catgggtgct ttcaaggaat gtaaagccct cgatcgcctc aaggtgctca tacattgctt
    20161 taagtacttc gtatga
//
LOCUS       DAONVQ010000018        19236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2094_length_19236_cov_4.702883, whole genome shotgun sequence.
ACCESSION   DAONVQ010000018 DAONVQ010000000
VERSION     DAONVQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 19236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19236
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2094_length_19236_cov_4.702883"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1583
                     /locus_tag="PLD98_02270"
     CDS             129..1583
                     /locus_tag="PLD98_02270"
                     /inference="COORDINATES: protein motif:HMM:NF002969.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HQA05107.1"
                     /translation="MRNICEISDKTFGDLPEKVLQIGEGNFLRAFAEWIIETGNRQGV
                     LSTSVLVCQPIEKGNCDLINAQSGIYTVYMRGISKNAVFEDMQKITCISRCINPYREY
                     EDLLEAARSEDLEVIISNTTEAGIAYKEGDRLTDTPPSSYPAKLTALLYERFSVFPSK
                     DKGVLILPVELIDDNGAKLKSIVLRYAKEWELGDEFISWLEEGCYFANTLVDRIVTGF
                     PADEYAEICAKMGYEDKLLVTCEPYLFWAIQCPKEWAEKFPIDKLGFNIIFADDISPY
                     RERKVRILNGAHTLSVLAAFLTGHEFVIDMMRDGLFSEFIRKAIFSEIIPTLSLPEKE
                     LVSFAESVLDRFSNHFIKHRLHDISLNSVSKFKARCLPSLLKYKEIKGALPDKLCFSL
                     AALIRFYKCRFEDGKYLGKDDSGREYEIRDGEDVKEFMRSAWESGDAAKAVLSNISFW
                     DMDLTEIQGLEEKVRGYLIDIESLGIRKALEKNF"
     gene            1594..>2882
                     /locus_tag="PLD98_02275"
     CDS             1594..>2882
                     /locus_tag="PLD98_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HQA05108.1"
                     /translation="MPKLIKIHPNDNCAVAVSEIKKGESIEGLSALSDIPFGHKIALF
                     DIAAGEAVIKYGYPIGVAIKEIKKGEHIHTHNLRTRLSGEIEYKYEPCGCSCDSAAKN
                     HDIPTFKGYRRNDGTAAIRNDIWIIPTVGCVNNIAKRLEKEARSALQSKYEGITGIHA
                     FTHPYGCSQLGDDHEFTRVILAGLAANPNAAGVLVLGLGCENNTLKSFMPLLENLNKP
                     VKAFNVQDVCDETDEGLALIEELCSLCCKAERTEIPVSELKIGYKCGGSDAFSGITAN
                     PLCGRLTDLLTSMGGTCVLTETPEMFGAETILMNRSCTFDVFEAQTKMINGFKEYFTS
                     YGQPIYENPSPGNKEGGITTLEEKSLGCIQKGGTAPVADVLSYGERIKKRGLTLLNGP
                     GNDIVSSTALTAAGVHMIIFTTGRGTPLGAPVPTIKMS"
     assembly_gap    2883..2892
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2893..3087
                     /locus_tag="PLD98_02280"
     CDS             <2893..3087
                     /locus_tag="PLD98_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HQA05109.1"
                     /translation="NTALYEKKPNWIDFNAGTLLEGDDFDKKLRELLDFVISVASGRQ
                     TNNEINDYREIAIWKNGVTL"
     gene            3132..4535
                     /gene="uxaC"
                     /locus_tag="PLD98_02285"
     CDS             3132..4535
                     /gene="uxaC"
                     /locus_tag="PLD98_02285"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HQA05110.1"
                     /translation="MKKFMDENFLLQTETAVKLFQLCKDEPIVDWHCHLSPKEIYENK
                     QPEDIAQLWLGGDHYKWRAMRSYGISEEYITGNKSGYEKFKAWAKVMPSLIGNPLYHW
                     THLELQRFFDIYTPLSEATADEIWEKANAKIAAGGFRPRDLITRSNVKSVFTTDDPAD
                     SLEYHKLLKEDKSFSCLVLPAFRPDKALNIDLPTFVPWLKDLEKAADMKIDSFEELKA
                     ALLKRIEFFAEMGCKATDHALGGVPFALSTPDELEAIFKKGKNGETLTQEEADKYKTA
                     LLRFLAKEYYKKGWAMEIHIGALRNNNTRMFEKLGPDTGFDSIDDRQIAYPLSRLLDS
                     IDKEGMLPKTILFTLNPKDNYVLGTMIGNFQSDEIGSKMQFGTAWWFNDNIDGMEAQI
                     KALANLGCLGKFIGMLTDSRSFLSYPRHEYFRRILCNFMGGLVENGEYPFDLPALERM
                     VKDISYYNAVKYFGLEL"
     gene            5028..5924
                     /locus_tag="PLD98_02290"
     CDS             5028..5924
                     /locus_tag="PLD98_02290"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HQA05111.1"
                     /translation="MTEINASPLGNDAQRKTLKKTKALNALVTGMLIPCLFAAAPLEL
                     AFSGRHTKTLAYEEFKAPAKTALYDVQSESSEEETENITMLSEPVSEVLDIAPSVPLA
                     EEETEHQSLIFRYQQLNGEIVLISEKNTGSTDKPEPNFDPPKKATVKKAAAKKTQSSA
                     KSAEVSISSSSVSVSGVPKAMSMTRLSGIEFDENGIPKKYKKVIEGKASAYTSYGNSR
                     TATGTVPRLGTVAVNPKIIPYGTKMWIVSLDGSVNYGFAVAEDTGGFIKWKNPRIVDL
                     FMPTKAQCRNWGVRGVRIYILE"
     gene            complement(6244..7080)
                     /locus_tag="PLD98_02295"
     CDS             complement(6244..7080)
                     /locus_tag="PLD98_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQA05112.1"
                     /translation="MLKSGQKLKSGGQIVLIPHELIYPNPNQPRKHFDYDELEGLAES
                     IRANGILQPLNVRQAENGTYELISGERRLRAARIVGLTRIPCIVMRANDEQTALYSLL
                     ENLQRQNLNFFEEAQAIETLMTEYNLSQESIAKKLGKSQSCLSNKLRLLRLTPDMREK
                     IMKAGLTERHARALLRLDTNEQRERALGIIADRRLNVAETDRLIGQMLKRSIQKRKPA
                     IKVFKDVRIFINTLNNAVDTMRKAGIEADSAKSETPEYIEYIVRIPKITSSKTDTISS
                     AV"
     gene            complement(7198..7914)
                     /gene="rsmG"
                     /locus_tag="PLD98_02300"
     CDS             complement(7198..7914)
                     /gene="rsmG"
                     /locus_tag="PLD98_02300"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HQA05113.1"
                     /translation="MTEFEIRLKELSNQAEIDLTEEMISRFSLYASLLKEWNEKMNLT
                     AITDDEGIAVKHFIDSLYLLKLVDIKEGNSLVDVGTGAGFPGVPLLIARPDLKVCLMD
                     STAKRLKFLETVLTELKLTADTVHIRAEEAGQNKTFRESFDIATARAVAPLHILSEYC
                     LPLVKKGGVFLSLKGKPESDEIERGEKAIKVLGGEKAQIMEYELINGDKRTLIITKKI
                     SQTPPKYPRTSAKLAKKPLG"
     gene            complement(7911..8570)
                     /gene="plsY"
                     /locus_tag="PLD98_02305"
     CDS             complement(7911..8570)
                     /gene="plsY"
                     /locus_tag="PLD98_02305"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HQA05114.1"
                     /translation="MKYFEMPGFALYALLAAVVGYLLGSVSFSVIASRLIAKDDVRNH
                     GSGNAGFTNMMRNYGKLPSAVTLIGDFSKGVAAVLLGGYILSKLSPDLKILGEVIGSV
                     SVFVGHVFPLFFGFRGGKGVLSTAGAVLIIDYRIFLILVAAFAVIALTSRIVSLASIS
                     TAVLLPILVFAFYRNDGGLKLLLIYSIIICLMVLITHRENIKRLLNGTEYRFKRKGKT
                     E"
     gene            complement(8595..10466)
                     /gene="mnmG"
                     /locus_tag="PLD98_02310"
     CDS             complement(8595..10466)
                     /gene="mnmG"
                     /locus_tag="PLD98_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQA05115.1"
                     /translation="MKFLAGDYQVAVIGAGHAGIEAALASARLGCRTVVFTLNLDWIA
                     NVACNPSIGGTSKGHLVREIDALGGEMGKAADSTLIQSRMLNSGKGPAVHSLRAQIDR
                     RKYSEYMKHTLEKCKNLDLKQAEIVDLKRTKDGRWELTTHLDAIYLADAVIISTGTFL
                     NGKIFVGDKSFSGGPDGMFPAVRLGEALKKLNIPLRRFKTGTPPRVNALSIDYSRLEA
                     QPGDEKIVPFSFETRRQLKNKVVCYITYTNEKTKQIILDNLHRSPLYSGEIEGVGPRY
                     CPSIEDKIVRFKDKERHQIFLEPCGLNTEEVYLQGMSSSLPEDVQYAFVRTIKGLENV
                     KIMRPAYAIEYDCVDPLYLKPTLELMYEPMLYGAGQFNGSSGYEEAAAQGIVAGINAA
                     RKVQGKSEIIFSRTESYIGTLIDDLVTKGCQDPYRMMTSRSEYRLVLRQDNADLRLTQ
                     KGYEIGLISEKRYKAFLKKKAAIEDEYKRMSKVIIPPIKEVNDKLQELGTAPIETGVK
                     LSELLKRPQVTYSDIKPFDKDAPDYDDEVFQQVEIMLKYEGYIKRQNAQIQEIHRLES
                     KLIPEDIDYSQIIGLRKEAIEKLSKIRPVNVGQASRISGVSPADISVLLIYLSRLGK"
     gene            10693..12675
                     /gene="ligA"
                     /locus_tag="PLD98_02315"
     CDS             10693..12675
                     /gene="ligA"
                     /locus_tag="PLD98_02315"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQA05116.1"
                     /translation="MDRTFAEKRAEELRKLIDKYSRQYYVENESEISDYEFDMLMNEL
                     KEIESRFPELKTPDSPTQRVGGSAAGQFESVVHPVPLESLQDLFSEGELIDFARKLKA
                     AYPEITFVTEPKIDGLSVSLEYTNGVFTRGATRGDGLVGENVTANLRTIKALPLRLTE
                     EIPYLVVRGEVFMPRESFYKLVRQQEEKGEKVFKNPRNAASGSLRQKNPAITAERELS
                     IFVFNIQRIDGVEVRTHAEGLELLRRLGFNVIPDYVVSDDIEGSLQAIRALGERREGL
                     PYDIDGAVVKVNQLALRQVIGSTSKFPKWAAAFKYPPEEKETVLKDIEINVGRTGILT
                     PTAILEPVLLGGTTVSRASLHNEDYIKERNIRIGDTVIVRKAGDIIPEVVKVTKHGEN
                     SAEYVYPTECPSCGSPVVREEDEAAVRCVNPDCPAQLLRGLIHFCSKEAMDIEGLGEA
                     VLTTFTQRGLIKGIADIYRLKAEEISVIPGMGKKSAENLLNAIEKSKQNPLSRLINAL
                     GIRNIGQRASKLLAARFKTMENLINATKEDILSIEGFGEIMAESIINFFSLPHVKELI
                     NELKDLSVNMTESEEEGKDRRFEGMTFVLTGALSKYTRDEASKIIESFGGKVSSSVSK
                     KTSVVLAGENAGSKLTKAQELGVKVINEEEFEKMIS"
     gene            complement(12703..13668)
                     /locus_tag="PLD98_02320"
     CDS             complement(12703..13668)
                     /locus_tag="PLD98_02320"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQA05117.1"
                     /translation="MKSFEIGKNDAGQRMDKFLSKAAPSLPKSLLYKYLRTKRIKLNG
                     KRAEPASRLNEGDIVELYINDEFFARKEYHYDFLKAPKKPDILYEDENILIINKPPGV
                     LCHPDGDNYTNNLIASVRRYLFEKGEYDPEKENSFAPALANRIDRNTGGLVMAAKNAE
                     TLRILNERIKNREITKKYLCVAEGAFKEKSGVLEGFISLNKSQNKATVTKKETEDSKA
                     IKTGYTVLKEKNGLSLLEIELFTGRTHQIRAQFAAIGHPLYGDTKYKGKAAGENSPYK
                     KQYLYAYKLEFSFKTDAGALNYLKDKVFTAPEPEFVAEFNNGKLK"
     gene            13833..14771
                     /locus_tag="PLD98_02325"
     CDS             13833..14771
                     /locus_tag="PLD98_02325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQA05118.1"
                     /translation="MKKVSLVVPCYNEADVLDLLYKEVTAVFDALPQYELELLLVDDG
                     SRDNTLEIMKRLAEKDSKVKFISFSRNFGKEAAMLAGLKYASGEYIGIIDADLQHSPD
                     LLPPMIEAIENEGYDVAAARRTDRAGEAKLKSALSEFFYKIYNKFSDVHLEESAQDFR
                     VMKRKVVEAIISLSEKNRFSKGIFTWVGFKTKWFEHENRQRAAGETKWSVRKLLKYAI
                     DGLLSFTNAPLRLPLYLGSVSVAVSILTFIGIIIAKIVNTSGSYGTVLIITAIMFFAG
                     IILQCLGIMGMYLARIYDESRDRPIFIVSETNIEKA"
     gene            14818..17952
                     /gene="ileS"
                     /locus_tag="PLD98_02330"
     CDS             14818..17952
                     /gene="ileS"
                     /locus_tag="PLD98_02330"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQA05119.1"
                     /translation="MAENEKNTFIEMEHEILKFWEENKCFEKLMKKNEGNEPFRFIDG
                     PITANNPMGIHHAWGRTLKDTFIRYKMKRGYSCRCQNGFDAQGLWVEVEVEKELGFKT
                     KKDIEAYGMDKFTRQCVDRVKKFSSIITEQSKRLGQWMDWDNSYYTHTDKNIEGIWHF
                     LKVCNEMGMIEREYRPMPWCPRCGTSLSEHEMTGSYKMLTHNSVFFKLPLKEIPSKIL
                     AWTTTPWTLSSNVALAVNPEIDYAEVRVKSDDQTLFLAKNAISRLGDDKLEVLRVVKG
                     SELVGLHYETCFPELEAQKDVEHKVVAWDEVAADEGTGIVHIAPGCGIEDYELGLKLG
                     LPQIMPVDDMGIFLSGFGFMSGKECHEVASEIFDRLKADGKLYKVEPYEHSYPVCWRC
                     KQEIIFRLVPAWYIRTEDIKPELIKAAQSVKWEPEANGKRMVDWLQNMGDWNISRKRY
                     YGLPLPFYPCECGHITVIGNRKELRERSVNPEVVDSLPELHRPWIDEVRIKCEKCGKE
                     VSRITEVGDVWLDAGIVPYSTLHYFDDREYWNKYFPADWVVEMREQVRLWFYSMLFMS
                     VVLEGRAPYKRVLCHNSVVKEDGGKFSKTGYMIKFDEAAENMGADTIRYLYAGAPVAN
                     DVRFGYNLGDEARRKLLAFSNIYTFIETYASIDKPVLEGYTPDVSAMTPTDKWLILRT
                     NEFIQRATECYEDFKTYALVKDFEVFVDDISNWYIRTNRRRFWKTGDVKDKTLAYWVL
                     TFALDAATRCMSPIIPFLTENIWQKLIRKLHPSAPISVHLSEWPEPFEGIKDTEVLEQ
                     TALARDVINTAMRLRNEQQLKVRQPLSTLYVCCSDEDAEKIKVFEKTILDELNIRKLE
                     YINDLSILQDSYLTVNFRSAGAVLKQDVNKLKAVLESLPADKMTKAAETVSKGGSVRF
                     NEFDMDLAPELFNVQTAPKKGIAHGEVSPEITVAIDTHLTDELIADGIVRDIVRQWQI
                     LRKDTGYSVEQRIIAAIKTADDFTKAAVENKSEYIKNELLADELIINGDGEFDADKTF
                     EVNDADVTIYVKSV"
     gene            18038..18238
                     /locus_tag="PLD98_02335"
     CDS             18038..18238
                     /locus_tag="PLD98_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HQA05120.1"
                     /translation="MTVDRNTKIGELLDADRTLAPIFLNMGMHCLGCPASRNETIEEA
                     CMVHGVDPDEILEQINEHLANK"
     gene            18240..18974
                     /locus_tag="PLD98_02340"
     CDS             18240..18974
                     /locus_tag="PLD98_02340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQA05121.1"
                     /translation="MQGSLRGGAAAPRLDKRLRMVANLLRHNRKLIDVGTDHGYLPAF
                     MVIMGYSPCAAATDINPGPLENARATIEKYGIADRVSLILSDGLDNVPPDGEEIVMAG
                     MGGELMCEIIGRAPWLKDSGKALVLQPMTKAEELRKYLADNGFVIEKETAVRDGSHAY
                     LALRAVYSGETKNVSKAYFYIGELLSGEISDDIIFYTEKLLSRIKHKADALNNTEDFK
                     EEAKELYEIYDEISEKLQVSLRDFNK"
     gene            19029..>19236
                     /locus_tag="PLD98_02345"
     CDS             19029..>19236
                     /locus_tag="PLD98_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQA05122.1"
                     /translation="MTVGEILNAIDSIAPFAGQEAWDNSGLVVGDFNQKVNTVIVALD
                     LTSEVLDHAKNMAAQVIITHHPPIF"
BASE COUNT         5107 a         4389 c         4737 g         4993 t
ORIGIN      
        1 aaacttacta aagaagccaa agtttattcc gatgaggaaa atcttgaaac ttgccttact
       61 tgacataatc tatatagaaa tatatgctaa atcagaaaag agtcaatctt tagcaatcgg
      121 agtgttttat gagaaatatt tgtgaaatat cggataaaac ctttggcgat ttgcccgaaa
      181 aggtgcttca aatcggcgaa ggcaattttc ttagagcctt tgcggagtgg ataatagaaa
      241 ccggcaacag gcagggcgtg ctttcaacca gcgtgctggt ctgtcagccc attgaaaagg
      301 gcaactgcga ccttatcaac gctcaaagcg gcatttatac cgtttacatg cgcggcatct
      361 caaaaaacgc ggtctttgag gatatgcaaa agataacctg catttcccgc tgtataaatc
      421 cttacaggga gtatgaggac ttgctggagg cggcccgcag cgaggatttg gaagtcatta
      481 tttcaaacac caccgaagcg ggaatcgcct acaaggaggg ggacagactg acagatactc
      541 ccccgtcgtc atatcccgcc aaacttaccg cgctgctgta tgagcgtttt tccgttttcc
      601 cgagcaagga taagggagtt ttgattctgc cagttgagct catagacgac aacggcgcga
      661 aactgaaatc cattgtttta aggtacgcga aggaatggga gctgggcgat gagttcatct
      721 catggctgga ggaaggctgt tattttgcca acacattggt tgacaggata gttacgggtt
      781 tccccgcaga cgagtacgcg gagatttgcg caaaaatggg ctatgaagat aagctcctgg
      841 taacttgcga gccctatctc ttctgggcta tccagtgccc gaaggagtgg gctgaaaaat
      901 tcccgattga taaattgggt tttaacatta ttttcgcaga cgatatttca ccttacagag
      961 aaagaaaggt gcgcatactt aacggcgcgc acactctaag cgtgcttgcg gctttcctta
     1021 cgggtcacga gtttgtgata gacatgatgc gtgacgggct tttctcggaa tttataagga
     1081 aggctatatt cagcgagatt attcctactt taagcctgcc cgaaaaggag cttgtcagct
     1141 tcgccgagtc ggtgcttgac aggttctcca atcactttat taagcatagg ctgcacgata
     1201 tttcactgaa ctccgtttca aaattcaagg caaggtgcct gccctctctg ctcaaataca
     1261 aagagattaa gggcgcgctc cccgataagc tctgcttctc ccttgccgct ctcattaggt
     1321 tctacaagtg ccgctttgag gacggaaaat acctcggcaa agacgacagc ggcagagagt
     1381 atgaaatcag agacggagag gatgtaaagg agtttatgcg ctccgcgtgg gaaagcggag
     1441 atgctgcgaa agccgttctt tcaaacatct ctttctggga tatggatttg accgaaattc
     1501 agggacttga ggaaaaggta aggggatatc ttattgacat tgaaagcctc ggaataagga
     1561 aagcactgga aaagaacttt taaaggagca aatatgccaa agcttataaa aatacacccg
     1621 aacgacaatt gcgcggtggc tgtaagtgaa ataaaaaagg gcgagagcat tgaggggctc
     1681 tccgcccttt cagacatacc cttcggacac aagattgccc tttttgatat tgccgcgggt
     1741 gaagcggtta taaagtacgg ctaccctatc ggcgtcgcca ttaaagaaat taaaaagggc
     1801 gagcatatac atacccacaa tttaagaaca aggctttcgg gggagattga gtacaaatac
     1861 gagccttgcg gctgttcctg cgactcggcc gcgaagaatc acgatattcc cacatttaag
     1921 ggatacagaa gaaacgacgg aaccgcggcg ataaggaatg atatctggat tatccccacc
     1981 gtcggctgcg tgaacaatat tgcaaagagg cttgaaaaag aggcaaggtc tgcgcttcaa
     2041 agcaaatacg agggcataac gggcattcac gcttttaccc acccttacgg ctgctctcag
     2101 cttggcgacg accatgagtt tacaagagta attcttgcgg ggcttgcggc taatccgaat
     2161 gccgctggcg tgctggtttt ggggctgggc tgcgagaaca acacgctcaa atcctttatg
     2221 ccccttttag aaaaccttaa taagcccgta aaggctttta atgttcagga tgtatgcgac
     2281 gaaacggacg agggcttggc tctaattgag gagctttgct ctctctgctg caaagcggaa
     2341 aggaccgaaa tccccgtcag cgagcttaaa ataggctaca aatgcggcgg ctccgacgcc
     2401 ttttcgggca ttaccgcaaa ccccctatgc ggcaggctga cggacctgtt gacctccatg
     2461 ggcggcactt gcgttctgac cgaaactccc gagatgttcg gcgcggagac catacttatg
     2521 aaccgctcat gcacatttga tgtgtttgag gctcaaacta agatgattaa cggctttaag
     2581 gagtatttta cttcttatgg tcagcccata tatgaaaacc cctccccagg aaacaaggag
     2641 ggaggaatca cgacccttga ggaaaagtcc ctcggctgca tacaaaaggg cggaaccgct
     2701 cccgttgccg atgtgctaag ttacggggag agaataaaaa agaggggcct tacgctctta
     2761 aacggccccg gaaacgacat tgtttcaagc actgccctga ccgcagcggg tgttcatatg
     2821 attatattca caacaggcag gggcactccc ctcggcgcgc cagtgcccac cataaagatg
     2881 tcnnnnnnnn nnaacactgc gctttatgag aaaaagccca actggattga ctttaacgcg
     2941 ggaacgcttc ttgagggcga tgactttgac aaaaaactca gggagctttt ggacttcgta
     3001 ataagcgtag ccagcggcag gcagacaaat aacgagatta acgactaccg cgaaatagct
     3061 atatggaaaa acggcgtgac gctttaggaa tcggcgttta cgctttaacg gataggaaag
     3121 gatgatttga gttgaaaaag tttatggacg agaactttct tttgcagacc gaaactgcgg
     3181 taaagctgtt ccagctctgc aaggatgagc ccattgttga ctggcactgc catttatccc
     3241 ccaaggagat atacgagaat aagcagcccg aggatattgc tcagctttgg ctgggcggcg
     3301 accattacaa atggagggca atgcgcagct acggcataag cgaggagtat atcacaggaa
     3361 acaaatcggg ctatgaaaag tttaaggcat gggcaaaggt aatgccgagc ctcatcggca
     3421 atccccttta ccactggact caccttgagc tgcagagatt cttcgatata tacactcccc
     3481 tttctgaggc cacagccgat gaaatctggg aaaaggcaaa cgccaaaatc gcagcgggcg
     3541 gttttagacc ccgcgacctt attacccgct ccaatgtaaa gagcgttttc accaccgacg
     3601 accccgccga cagccttgag tatcacaaac ttcttaaaga ggataagagc ttcagctgcc
     3661 ttgttctgcc cgcattccgt cccgacaagg ctcttaacat agatttgccc acctttgttc
     3721 cgtggcttaa agaccttgaa aaagccgccg atatgaaaat tgacagcttt gaggagctta
     3781 aagctgccct cttaaaaaga attgaattct ttgctgaaat gggctgcaag gcgacagacc
     3841 atgctttggg cggcgttccc tttgctctct caacccccga cgagcttgag gcaatcttta
     3901 aaaagggcaa aaacggcgaa acacttacac aggaggaggc ggacaaatac aagacagcgc
     3961 ttctccggtt cctcgcaaag gaatactaca aaaagggctg ggcgatggaa attcacatcg
     4021 gcgctctcag aaacaacaac acaaggatgt ttgaaaagct gggtcccgat actggctttg
     4081 actccattga cgacaggcag attgcctatc ccctctcaag gctgcttgac agcatagaca
     4141 aggagggcat gctgccgaaa accatactct ttacactcaa tcccaaggac aactatgttc
     4201 tcggcacaat gataggcaac ttccagagcg atgagatagg ctcaaagatg cagttcggca
     4261 ccgcttggtg gttcaatgac aatatcgacg gcatggaggc tcagataaag gcgctcgcaa
     4321 atctcggctg cctcggcaaa tttatcggca tgctcaccga ctcccgctca ttcctttcct
     4381 accccagaca cgagtatttc agaaggatac tctgcaactt tatgggaggt cttgttgaga
     4441 acggagaata tccctttgac ctgcccgccc ttgagagaat ggtcaaagac atctcctatt
     4501 acaacgctgt aaaatacttc ggtttggagc tgtaataaac ctcaaaaata atgaaaagcc
     4561 cgcactaaaa tgcgcgggct tttttctttt gcgaaaaatt atattacatt atatatacgc
     4621 ttttctcctt ccatttttta tgcaatttat cgtcgatttt accaatggaa aacactgtcc
     4681 gattattacc ccagtgtaat aaattcacct caaattatta caaattgtaa ccattttttc
     4741 agctcttgta acccctttgt aacgaattgc aagatttctc ttttttgaac aaaagcaatt
     4801 ggaattttat gggatttttc ggcttttttc ggggaaaaaa tacggatttt cccattattt
     4861 cccttgtata tacaaaaact aattgtgcat actgcacaat ttatgattct caaaaaagtt
     4921 gacaatcgga caagctgtaa atataatgac gacacctttt gcctataatg tatcaagccc
     4981 gcaaaatccc atttttgcgg tgtactatga tatctgagga gatttttatg acagaaatta
     5041 atgcgtcccc attaggcaat gacgcacagc gcaagaccct caaaaagaca aaagcgctga
     5101 atgccttagt aaccgggatg ctgataccat gtctgttcgc cgcggctcct ttggagctgg
     5161 cattctcagg acgccacaca aaaacactcg cttatgagga gtttaaggct cctgcgaaaa
     5221 ccgctttgta tgatgtgcag tctgaaagca gtgaagaaga gacggaaaac attacaatgc
     5281 tttcagaacc cgtatcggaa gttcttgaca tcgctccttc cgtaccttta gcagaagaag
     5341 aaaccgagca tcaatcgctg attttcaggt atcagcagct gaacggtgaa attgttctta
     5401 tcagcgaaaa gaatacggga agcaccgaca agcccgaacc gaattttgac ccgcccaaaa
     5461 aggcgaccgt taaaaaggct gccgctaaaa agacccagag cagcgcaaaa tccgcagagg
     5521 tttcaatatc ctcatcatcc gtttccgtat cgggcgttcc caaggctatg tcaatgacca
     5581 ggctgtcggg aattgagttt gacgaaaacg gaatccccaa aaagtacaaa aaggtcattg
     5641 agggcaaggc atccgcctac accagctacg gcaactcaag gaccgctacg ggaacagtac
     5701 cgaggcttgg caccgtagcc gtaaacccaa agattattcc ctacggcaca aaaatgtgga
     5761 ttgtatccct tgacggctcc gttaactacg gatttgcggt tgctgaagac acaggcggct
     5821 ttatcaagtg gaagaatccg agaatagtcg atttattcat gcccacaaag gcccaatgca
     5881 gaaactgggg cgttagaggt gtaaggatat acatccttga gtaaaagcga aatttgaccc
     5941 ctttatgctg aaatttcggg ctgttgtgaa aaatcccgca actcaaagcg gtttataagc
     6001 actaaatgac gaatatcaga cactttgaac cctgcgtaaa tcacgcaggg ttttcctttt
     6061 tgggccgaaa gcaaagagtt agtttgcgcg cagtaaaatg ggcgtgtttc ttaaagattt
     6121 aaggtgaaaa agaccgcaat aacacacaaa gaggacagac aagctgtcct ctttgtgtgg
     6181 atgtggggtg tgaaagagaa tgaatatatt atagcggatg gaccctcaaa acttatggaa
     6241 tctctataca gcggaggaaa tcgtatcggt tttgcttgag gttattttgg gaattctgac
     6301 tatgtactct atatattcgg gagtttcgct ttttgcggag tctgcctcta tgcccgcttt
     6361 gcgcattgtg tcaaccgcgt tgttaagggt gtttataaag attctgacat ccttaaagac
     6421 tttgattgcg ggttttctct tttggatgga gcgcttgagc atctgaccta tgagcctgtc
     6481 cgtttcggcg acattgagcc ttctgtcggc tattatgccc agcgccctct ccctttgctc
     6541 gttggtgtca agtcttaaaa gcgctctggc gtgacgctcg gtgagacccg ccttcattat
     6601 tttctcccgc atatcggggg taagtcttaa aagtcttaat ttgttcgaaa ggcaggactg
     6661 ggatttgccc agtttcttcg ctatgctctc ctggcttaaa ttgtactcgg tcatcagagt
     6721 ttctatagcc tgagcctctt caaagaaatt aaggttctgc ctttgaaggt tctctaaaag
     6781 ggagtatagg gcggtctgtt cgtcgtttgc cctcattact atacagggga ttcgggtcag
     6841 ccccactatt ctcgcggctc tgagccttct ttcgccggag ataagctcgt atgtaccgtt
     6901 ttctgcttgg cggacattca ggggttggag aattccgttc gctcttatgg actccgcaag
     6961 cccctccagc tcgtcgtagt cgaaatgctt tctgggctgg ttcggattgg ggtaaatcag
     7021 ctcatgcgga ataagaacga tttgcccgcc ggatttaagt ttctggccgg atttaagcat
     7081 atcaaaaccc ccgcctcttt ttgtcgtcct ttttctgaga ttagattacc acaaaataaa
     7141 tggaagaaaa agggaaatcg gtcgggggaa tacaactctc ttcgccagaa aatctgatta
     7201 tcccaatggt tttttggcga gtttggcaga tgtgcgggga tattttggcg gggtttgcga
     7261 tatttttttt gtaataatca gtgtcctttt atcgccgttg ataagctcat attccataat
     7321 ctgagccttt tcgccgccca ggacttttat tgccttctct cccctttcga tttcgtcgct
     7381 ttcgggcttg cctttaaggg agagaaagac gccgcccttt ttaacaaggg gcaggcagta
     7441 ttcggagagt atgtgcaagg gggcgaccgc tcttgccgtg gcaatatcaa aactctctct
     7501 gaatgttttg ttctgacccg cctcctcggc tcttatgtga actgtgtccg cggtgagctt
     7561 caattccgtg agcaccgttt caaggaattt aagccgcttt gcggtggaat ccataaggca
     7621 gactttaagg tcggggcggg ctatcagcag cggaactccg ggaaaacccg ctcccgtacc
     7681 aacatcaaca aggctgttgc cctctttaat atccacgagt ttcaaaagat acaggctgtc
     7741 aataaagtgc tttacggcaa tgccctcgtc gtcggtaatt gccgtaaggt tcattttttc
     7801 gttccattcc tttaatagcg aggcgtagag gctaaagcgg gaaatcattt cctcggttaa
     7861 gtctatttcc gcttggttag atagttcttt gagccttatt tcaaattcgg tcattcggtt
     7921 ttccctttcc gcttaaatct gtattccgtg ccgtttaaaa gccgctttat attctccctg
     7981 tgggtgataa gcaccattag gcagatgatt attgaatata tgagcagcag tttcagtccc
     8041 ccgtcattcc tgtaaaaagc gaatacgagt atggggagaa gaaccgccgt gctgatggac
     8101 gcgagggaca cgattcttga tgttagcgct atgactgcga atgccgcaac gagtattaaa
     8161 aatattctat agtcgataat gagcaccgcg cctgcggttg agagaactcc ttttcctcct
     8221 ctgaagccga agaacagcgg aaagacatga cccacaaaga cggaaacgct gcctatcact
     8281 tctcccagaa ttttaaggtc cggggacagc tttgacagta tatagccgcc caaaagaacc
     8341 gcggctacac ctttggagaa gtcgcctatc aaagttactg ccgaagggag ctttccgtaa
     8401 ttgcgcatca tatttgtaaa acctgcgttg ccgctgccgt ggtttcgtac atcgtccttt
     8461 gcgataagtc ttgaggctat taccgaaaag ctgacgctgc cgagaaggta gccgacgaca
     8521 gccgccaata acgcataaag cgcaaagccg ggcatttcaa agtatttcat tttatcctct
     8581 tcccaaaagt taatttattt gccgagcctt gacaggtaga ttaaaagaac cgaaatgtcc
     8641 gcggggctga cgcccgaaat tcttgacgcc tgacccacat ttacggggcg gatttttgaa
     8701 agtttctcaa tcgcctcttt cctgagtcct ataatctgag agtagtcgat gtcctcgggg
     8761 ataagctttg actcaagcct gtgaatctcc tgaatctggg cgttctgtct tttgatatag
     8821 ccctcgtatt tgagcattat ctctacctgc tgaaagactt cgtcatcgta atcgggggcg
     8881 tccttgtcaa agggttttat atccgaatat gtgacctgcg ggcgctttag aagctccgac
     8941 agctttactc ccgtttcaat tggggcggtt ccgagctcct gcagcttgtc gtttacttcc
     9001 tttataggcg ggatgatgac cttgctcatt cttttgtatt cgtcctctat tgccgccttt
     9061 ttctttaaaa acgccttgta gcgtttttcg gagataaggc cgatttcata gcccttttga
     9121 gttaagcgca ggtccgcgtt gtcctgcctt agaacaagcc tgtactcgga gcgggaggtc
     9181 atcattctgt aggggtcctg acatcccttt gtgacaaggt cgtcgattag cgttccgata
     9241 tatgattcgg ttcttgagaa tattatttca ctttttccct gaacctttct cgcggcgttg
     9301 attccagcga ctattccctg agccgctgcc tcctcatagc cgctggagcc gttgaactga
     9361 ccagcgccgt agagcatggg ctcatacata agctcaaggg tgggtttcaa atacaagggg
     9421 tccacgcagt cgtactctat ggcgtaggcg gggcgcatga tttttacatt ttcaagaccc
     9481 ttgattgttc ttacaaaagc gtactgcaca tcctcgggca gagaggagga catgccctga
     9541 aggtaaacct cctcggtatt cagtccgcag ggctcaagga aaatctgatg cctttcctta
     9601 tccttaaacc ttacgatttt gtcctctatg ctggggcagt atctgggtcc cacgccctca
     9661 atttcgccgc tgtaaagagg cgaacggtgg aggttatcaa ggataatctg ctttgttttc
     9721 tcgttggtgt aggtaatata gcaaaccacc ttgtttttaa gctgccttct tgtttcaaag
     9781 gagaatggca cgattttctc gtcccccggc tgagcctcaa ggcgggaata atctatgctg
     9841 agggcgttta ccctcggcgg ggtgcctgtc ttaaagcgtc ttaaagggat attcagcttt
     9901 ttaagggcct cgccgagtct tacagcgggg aacattccgt cgggaccgcc gctgaaggac
     9961 ttgtcgccca caaagatttt gccgtttagg aatgtgcctg tcgatattat taccgcgtcg
    10021 gcaaggtata tggcgtcaag atgggtggta agctcccatc tgccgtcttt ggttctttta
    10081 agatctacaa tttcagcctg ctttaaatca agattcttgc acttttcaag ggtgtgcttc
    10141 atatattcgc tgtatttgcg tctgtcaatc tgcgctctga gggagtgaac ggcgggaccc
    10201 ttgccggagt ttaacattct gctttggatg agggtgctgt ccgccgcctt gcccatctcg
    10261 ccgcccaaag cgtcgatttc ccttacaagg tgaccctttg aggtgccgcc gatggacggg
    10321 ttgcaggcga cattcgcaat ccagtcaaga tttaatgtaa agacaacggt tctgcagcca
    10381 agccttgcgg aagcgagtgc cgcctcgatt cccgcatgac ccgcgccgat aaccgcaacc
    10441 tgataatctc ctgctaaaaa cttcattttt catttccttt gctgtgcacg aattagtttc
    10501 cgctcaaatt aaatatttta tcacagctcg tcgttttttc aaagcattta tgctgcttta
    10561 gaagattttt tgtatctttt caattccttt gttaaatagt atcattgtaa tgagcttaaa
    10621 gttagaacag caaattaaga tttgattact ttttgatata gaaatattaa tttttcgaga
    10681 aaggaaatta atatggacag gactttcgca gaaaaaaggg ctgaggaatt aagaaagctg
    10741 attgacaaat acagccgtca atattatgtg gagaacgaga gcgaaatatc ggactatgag
    10801 ttcgatatgc tgatgaatga gctgaaagaa atagagtccc ggttccccga attaaaaacg
    10861 cccgactccc ctactcagag ggtaggcggc agcgcggcgg gtcagtttga aagcgttgtc
    10921 cacccagtgc cattggagag tttgcaggat ttgttctcag agggagagct tatagatttt
    10981 gcaaggaagc ttaaagccgc atatccagaa atcacttttg tgaccgagcc gaaaatcgac
    11041 ggtctttccg tttcgcttga atacacaaac ggagttttca caaggggcgc cacaaggggc
    11101 gacggtcttg tgggcgaaaa tgttacggcg aacctgcgca caattaaggc tctgccttta
    11161 aggcttactg aggaaatccc ctatcttgtg gtcaggggcg aggtttttat gccaagagag
    11221 agcttttata agctggtacg ccagcaggag gaaaaagggg aaaaggtttt caagaatccc
    11281 cgaaacgccg cgtcgggaag tctgaggcag aaaaaccccg caatcacagc ggagagggag
    11341 cttagcattt tcgtattcaa cattcagagg atagacggcg tggaggtaag aacccatgcc
    11401 gaaggtttgg agctgttacg gcgtcttggc tttaatgtca ttcccgatta tgtggtttcc
    11461 gatgatattg agggctcgct ccaggctata agagcgctgg gcgagaggcg cgagggcctg
    11521 ccgtatgaca ttgacggagc tgtggttaag gtaaatcagc ttgcgctccg tcaggttatc
    11581 ggcagcacct ccaaattccc caaatgggcg gcggcgttta aatatccgcc cgaggaaaag
    11641 gagactgtgc tcaaagacat tgaaataaat gtgggcagaa cgggaatttt gaccccgaca
    11701 gccattctgg agcccgtgct ccttggcgga acgacggtca gcagggccag cctgcataac
    11761 gaggactata ttaaagaaag aaacatccgc ataggcgata ccgttatagt cagaaaagcg
    11821 ggtgatatta tccccgaagt tgtgaaggtt acaaagcatg gggaaaacag cgccgagtat
    11881 gtttacccaa cagaatgccc ttcatgcggc agccccgttg ttagggaaga ggatgaggcg
    11941 gccgtaaggt gcgtaaaccc cgactgcccc gctcagcttt tgaggggtct tattcacttc
    12001 tgctcaaaag aggctatgga cattgagggt ctgggcgagg cggttctgac aacatttaca
    12061 cagcggggtc tgattaaggg aatcgccgac atatacaggc taaaggctga ggagataagc
    12121 gtcatccccg gcatgggcaa aaagtccgcg gagaacctat tgaacgccat agaaaaatca
    12181 aagcagaatc ccctctcacg ccttataaac gctttgggaa taagaaatat cggtcagagg
    12241 gcgtcaaaac tgcttgccgc aaggtttaag acaatggaaa acctgattaa cgccactaaa
    12301 gaggacattc tctccattga gggctttggc gagattatgg ctgagagcat tataaacttc
    12361 ttctccctgc cccatgttaa ggagcttata aatgagctta aagacctttc ggtcaacatg
    12421 acggagtccg aggaggaggg caaagacagg cgctttgagg gcatgacctt tgttctgacg
    12481 ggcgcgctct caaaatatac ccgtgacgag gcatcaaaaa taatagagag ttttggcggc
    12541 aaggtgtcct catcggtgtc taaaaagacc tccgtggtgc ttgcgggcga gaacgccggc
    12601 agcaagctga caaaggctca ggagctgggt gtcaaagtta taaacgaaga agagtttgaa
    12661 aagatgattt cataaaaaga aggtgagcat aaagctcacc ttttacttta gtttaccgtt
    12721 attaaattcg gcgacaaact ccggttcggg ggcggtaaag accttgtctt taaggtagtt
    12781 taaagcaccc gcgtctgttt taaaggaaaa ctccagttta taggcataga gatactgctt
    12841 tttgtagggg ctgttttcgc cggcggcctt gcccttgtat tttgtgtcgc cgtatagcgg
    12901 gtgaccgatg gcggcaaact gggctctgat ctgatgcgtc ctgcccgtaa aaagctcgat
    12961 ttctaataga ctaagaccgt ttttctcttt taaaaccgta tagcccgttt taatcgcctt
    13021 tgagtcctca gtttcttttt tggtaacagt cgctttgttt tggctcttgt taagggaaat
    13081 gaaaccttcc aaaactcccg acttttcttt aaaagcgccc tctgcgacgc agagatattt
    13141 ttttgttatc tccctgtttt ttattctctc attgaggatt ctcagcgtct cggcgttctt
    13201 tgccgccatt acaagcccgc ccgtgttgcg gtctattctg tttgcaaggg cgggggcaaa
    13261 ggagttttct ttttcggggt cgtactcccc cttctcgaaa aggtagcggc gaacgcttgc
    13321 tataagatta tttgtgtaat tgtcaccgtc cggatggcac agaactcccg gaggcttgtt
    13381 tataataagg atgttttcgt cctcatagag aatatccggt ttcttggggg ctttcaggaa
    13441 atcataatgg tattccttgc gggcaaaaaa ctcatcattt atataaagct ctacaatatc
    13501 cccttcatta aggcggctgg cgggctcagc cctttttccg ttgagcttta ttcttttggt
    13561 tctgaggtat ttgtataaaa ggctttttgg cagtgagggc gcggctttgg atagaaactt
    13621 gtccattctc tgacctgcgt cgtttttgcc tatttcaaag cttttcagat taatttctcc
    13681 catcctttgg tttgaactta ttatatcata aaacttcata gcttaattta caaacgaaaa
    13741 attaatagtt ttgcataaat atgtttgttt ttttgtcatt attatgctac aatatcggat
    13801 gaatttgtgt cctgccgaaa ggttgtgtat ggatgaaaaa ggtatcgctc gtggttccct
    13861 gctacaacga agcggatgtc ttagaccttt tatataaaga agttaccgcg gtttttgacg
    13921 cgctgcccca gtacgaactt gagcttttgc tggttgacga cggcagcagg gacaatactc
    13981 ttgagataat gaaaaggctt gccgaaaagg actctaaggt taagtttatt tctttttcaa
    14041 ggaatttcgg caaagaagct gctatgctgg cgggtttaaa atacgcgagc ggagaatata
    14101 taggaataat cgacgcggac cttcagcact cacccgattt gctgcctccc atgattgagg
    14161 caattgagaa cgagggctac gatgtggccg ccgcgagaag gaccgacaga gccggcgagg
    14221 caaagctcaa aagcgctctt tcggagtttt tctataagat atataataag ttttccgatg
    14281 tgcaccttga ggaaagcgct caggacttca gagtcatgaa gcgcaaagtt gtggaggcga
    14341 ttatatcgct gtccgagaaa aacagattct caaagggaat tttcacttgg gtgggcttta
    14401 aaaccaagtg gttcgagcac gaaaacaggc agagagccgc gggagaaaca aagtggtcgg
    14461 tcagaaagct gttaaaatac gctattgacg gacttttgtc ctttacaaac gctccgctga
    14521 gactcccctt gtatttggga agcgtcagcg tcgccgtttc catactgacc ttcatcggaa
    14581 ttataatcgc aaagatagta aacacttcgg gctcatacgg tactgtcctt ataattaccg
    14641 caattatgtt ttttgcaggt ataattttgc agtgtcttgg cattatggga atgtaccttg
    14701 caagaatata tgacgagtca agagacagac ctattttcat tgtaagcgaa acaaacatag
    14761 aaaaagctta aacgcacctg tatatatttt tattctatct tttcggaggg aacatctttg
    14821 gcagagaatg agaaaaacac ttttattgag atggagcatg aaatcctaaa gttttgggaa
    14881 gagaataaat gctttgaaaa gctgatgaag aagaatgagg gcaacgagcc cttccgcttt
    14941 attgacggtc ccattacggc caacaacccc atgggcattc accacgcatg gggcagaacg
    15001 ctgaaggaca ccttcatccg ctataagatg aaaaggggct attcctgccg ctgccagaac
    15061 ggctttgacg ctcagggtct ttgggttgag gtcgaggtcg aaaaggagct gggctttaag
    15121 acaaagaagg atattgaagc ctacggcatg gacaagttca cccgtcagtg cgttgacaga
    15181 gtaaagaagt tctcgtccat cattaccgag cagtcaaaga ggctcggtca gtggatggac
    15241 tgggataact catattatac acataccgat aagaacattg agggcatttg gcacttcctt
    15301 aaagtctgca atgaaatggg tatgattgag cgcgagtacc gtcccatgcc ctggtgcccc
    15361 cgctgcggaa catccctttc cgagcatgag atgacgggct cctacaaaat gctcactcac
    15421 aattcggtat tttttaaact ccccttaaag gaaatcccct ctaagattct tgcttggaca
    15481 accactccgt ggacgctttc ctcgaatgtg gctcttgccg ttaaccccga aatagattat
    15541 gcggaagtta gagttaagag cgacgaccag acattgttcc tcgctaagaa cgcaatctca
    15601 aggcttggcg acgacaagct ggaggttctg cgcgttgtta agggctctga gcttgtgggt
    15661 ctgcattacg agacttgctt ccccgagctt gaggctcaga aagatgttga gcataaggtt
    15721 gtcgcttggg acgaggttgc agccgacgaa ggtacgggca tcgttcacat cgctcccggc
    15781 tgcggtatcg aggactatga gctgggtctg aaactcggtc tgcctcagat tatgcccgtt
    15841 gacgatatgg gtatattcct cagtggcttc ggcttcatgt ccggcaagga gtgccatgag
    15901 gtcgcaagcg agattttcga cagacttaaa gcagacggaa aactttacaa ggtagagcct
    15961 tatgaacaca gctaccccgt atgctggcgc tgcaagcagg agattatctt caggcttgtt
    16021 cccgcatggt atataagaac agaggatatt aagcccgagc ttattaaggc tgctcagtcg
    16081 gtcaagtggg agccggaggc aaacggcaag agaatggttg actggctgca gaacatgggc
    16141 gactggaata tttcaagaaa gcgttactac ggtctgcccc tgcctttcta cccctgcgaa
    16201 tgcggtcaca ttaccgttat cggcaaccgc aaggagctga gggaaagaag cgtaaacccc
    16261 gaggttgtgg actctctgcc cgagctccat cgtccgtgga ttgacgaggt acgcattaag
    16321 tgcgaaaagt gcggcaaaga ggtttcaaga attaccgagg tcggcgatgt ttggcttgac
    16381 gcgggcatcg tcccctactc cacactccac tactttgacg acagagagta ctggaacaag
    16441 tacttccccg ccgactgggt cgttgagatg agggagcagg taaggctctg gttctactca
    16501 atgctcttta tgagcgtggt tcttgaaggt cgcgctccgt ataagcgtgt tctttgccac
    16561 aactcggtcg taaaagagga cggcggaaag ttctccaaga caggctatat gattaagttc
    16621 gacgaggccg cggagaatat gggcgcagac acaatccgct atctgtacgc tggcgctccc
    16681 gtcgcgaacg atgtacgctt cggctacaat ctcggcgacg aggcaagacg caagctgctt
    16741 gctttcagca atatctatac ctttatcgaa acttacgcaa gcatagacaa gcccgtactt
    16801 gagggctaca ctcccgatgt atccgccatg actcccaccg acaagtggct gatactcagg
    16861 acaaatgagt ttattcagag ggctacagag tgctacgaag actttaagac ttatgccctt
    16921 gtcaaggact ttgaggtctt tgttgacgat atttccaact ggtatatcag aaccaaccgc
    16981 cgccgtttct ggaagacagg cgatgttaag gacaagaccc ttgcatactg ggtactgacc
    17041 tttgctcttg acgcggctac ccgctgcatg tcgccgatta tccccttcct gacggagaac
    17101 atctggcaga agcttatcag aaagctccat ccctccgctc cgatttcagt tcacctctcc
    17161 gagtggcccg aaccctttga gggcattaag gatactgagg ttctggagca gacggctctc
    17221 gcaagggatg tcatcaacac cgctatgcgt cttagaaacg agcagcaact taaagttcgt
    17281 cagcccctct caacccttta tgtatgctgc agcgacgagg acgctgagaa gataaaggta
    17341 tttgaaaaga caatccttga cgagctgaat atcagaaagc tcgaatatat caatgatttg
    17401 tccattttac aggacagcta ccttaccgta aacttcagaa gcgcgggcgc ggttttaaag
    17461 caggatgtaa acaagctgaa ggctgtgctt gaatctctcc ccgctgacaa gatgactaag
    17521 gccgcggaga cggtttcaaa gggcggcagt gtcagattca atgaatttga catggacctt
    17581 gctcccgagc tctttaatgt ccagaccgct cccaagaagg gcattgccca cggcgaggta
    17641 agccccgaaa tcaccgtagc tatcgacact caccttaccg atgagcttat tgccgacggt
    17701 attgtaagag atatagtaag acagtggcag attttaagaa aagacaccgg ctactccgtt
    17761 gaacagagaa ttatcgcagc cattaaaact gccgatgact ttacaaaagc cgctgttgag
    17821 aataagagcg aatatatcaa gaacgaactg cttgccgatg agcttatcat caacggcgac
    17881 ggcgagttcg acgcggacaa gacctttgag gtcaatgacg cggatgtcac catttatgta
    17941 aaatccgttt aaggcttatt tacaagtttt gcataagggt gtattatgga ttcagggtaa
    18001 aataaccctg tagactgctt tcactggagg tttaattatg acagttgatc gaaatacaaa
    18061 aatcggcgag ctgcttgacg ctgacagaac actggctccc atctttttaa acatgggcat
    18121 gcactgcctc ggctgccccg cttcgagaaa cgagacaatc gaagaggctt gcatggttca
    18181 cggcgtcgac cctgacgaga ttctcgagca aatcaatgag catcttgcaa acaaataata
    18241 tgcagggaag tttaagaggc ggggccgctg cgccccgcct tgataaaagg ctcagaatgg
    18301 tggcaaatct tttgcgccac aacagaaaac ttattgatgt cggcaccgat cacgggtatc
    18361 tgccggcatt tatggttatt atgggctatt ctccttgcgc tgccgcgacg gacataaacc
    18421 ccggtccctt ggagaacgcg agggcgacaa ttgagaaata cggcatagcg gatagggtat
    18481 cgctgatttt gtccgacggg cttgataatg tccctcccga cggcgaagaa atcgttatgg
    18541 cgggcatggg cggagagctg atgtgcgaaa tcatcggcag agcgccttgg ctaaaggaca
    18601 gcggcaaggc tcttgtgctc cagcccatga caaaggcaga ggaattaaga aaataccttg
    18661 cagataacgg ctttgttatc gaaaaagaaa ccgctgttcg tgacggctcc catgcgtatt
    18721 tagctctaag ggctgtttat tcgggagaaa ctaaaaatgt cagcaaagcc tatttttata
    18781 ttggcgagct tttaagcggt gaaatatcgg acgatattat tttttatacg gaaaaactgc
    18841 tttcaaggat taagcacaaa gcagacgctc ttaataacac cgaagatttt aaagaagaag
    18901 cgaaagagct ttatgaaatt tatgatgaaa tttccgaaaa gctacaagtc agtttacggg
    18961 atttcaataa ataattgcgc ccctctattg gaatactcat aaaaactaaa cccattcagg
    19021 aggtttttat gaccgttggc gaaattttaa atgctataga ttcaattgcg ccgtttgccg
    19081 gtcaagaggc ttgggacaac agcggtcttg tggttggcga ctttaatcaa aaggtaaata
    19141 cagtcatagt cgctcttgat ttgaccagcg aggttctgga ccacgcgaaa aacatggcgg
    19201 ctcaggtaat catcacccat catccgccca tatttg
//
LOCUS       DAONVQ010000019        18806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2158_length_18806_cov_5.086449, whole genome shotgun sequence.
ACCESSION   DAONVQ010000019 DAONVQ010000000
VERSION     DAONVQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 18806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18806
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2158_length_18806_cov_5.086449"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLD98_02350"
     CDS             complement(<1..226)
                     /locus_tag="PLD98_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQA05123.1"
                     /translation="MEKYIKNIAHETPLSLLDEVSYQPGQIVSKTLAQNQYNSVTLFA
                     FDKGEEISTHASDGDAIVICLDGQGEITIDG"
     gene            complement(237..1040)
                     /locus_tag="PLD98_02355"
     CDS             complement(237..1040)
                     /locus_tag="PLD98_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQA05124.1"
                     /translation="MVRQIIKIDEELCNGCGLCAQACHEGAIGIVDGKAKLLRDDYCD
                     GLGNCLPVCPTGAISFETREALPFDEEAVKAKTEGKNADTEEKKTPERKPMFGGCPGS
                     MARAIVREEKEDNLPCGCPGSQSKTLERGEESVNAQNAPAAPRESRLMQWPVQIQLVP
                     VNAPYFNGAKLLIAADCTAFAYGDFHERFIKNHITIIGCPKLDTVDYSQKLEAIIRNN
                     DIKSITIVRMEVPCCAGIANAAITALRNSGKMIPWQVVTISTDGEIIDE"
     gene            1148..1816
                     /locus_tag="PLD98_02360"
     CDS             1148..1816
                     /locus_tag="PLD98_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQA05125.1"
                     /translation="MKEYIDLLSKNPLFKGIDKGDIGFLLDCLSAKTKTYYKDEPIFE
                     AGSRPEYVGIVLSGSAFVLQEDYWGNRNILSNIGVGGIFGEAFSCAEAAALPVSVIAK
                     EDSVILLINVKKILTICSTACAFHTSLIMNLMRILANKNRYLTRKIEHITKKTTREKV
                     LSFLSECAVQGGKNTFEIEFNRQELADYLSVERSALSAVLSKMKEEGIIDYYKNTFRL
                     TDKK"
     gene            complement(1843..2688)
                     /locus_tag="PLD98_02365"
     CDS             complement(1843..2688)
                     /locus_tag="PLD98_02365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQA05126.1"
                     /translation="MNSKPIVAIMYDFDKTLSPGNMQDYAFIPGIGKTADEFWRLCKE
                     YETANNMDPILAYMYYMVKSSRGKMLITREIFRKLGESVDFFDGVETWFERVNSFGES
                     LGLTVEHYIISSGLKEIIEGTKIAKHFKRIYAAEFCYDEEQVPIWPAMAVNYTSKTQF
                     LYRINKGVLDVTDHDSLNRYTTDSKRRVPFKNMIYIGDGYTDVPCMKLCKVNGGHSIA
                     VYQNDDRKIVNELIEQGRVDLVAPADYSEGSELESKVFRILEQIKTYNAVLKLHLKDM
                     DDAKA"
     gene            complement(3342..4004)
                     /locus_tag="PLD98_02370"
     CDS             complement(3342..4004)
                     /locus_tag="PLD98_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05127.1"
                     /translation="MKKVKDYNESIHFYGRLWMVFILIVFIGVPLSMCVYYNAWPKPA
                     AVLKGLLPIAMFYYPTAVVEVLTYAPLLGTGGMYLSFITGDISNLKLPCAIAAMNAAK
                     VKPNSEEGEIISTISIAASSIATIVIVAVCVFFLRPIIPIITDESSIFAPAFSQVLPA
                     LFGSILTAYLLKYPKTVVAPLAVILIILIFAGQMSVGILIPIGVVVAILAAHLMYKKN
                     LI"
     gene            complement(4048..4830)
                     /locus_tag="PLD98_02375"
     CDS             complement(4048..4830)
                     /locus_tag="PLD98_02375"
                     /inference="COORDINATES: protein motif:HMM:NF027796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HQA05128.1"
                     /translation="MTDFKEAGFMYAMGLVVAVIVVAQAVFFLVKAWRRGKELGITVK
                     DMKNTVVTSVMFSITPALSIVATVVALAGALGLVLPWVRTLIIGNISFETAAASAAME
                     AYGLGGLTSEVTDPEVFSAIAWVMTLGSMLPLVIIPIFLKRIQKKMGKALSKDTKWAD
                     VISSAAFVGLICAFVGRAILGQGNVDVSPEVLTDGAGVLSVTALVFSVLFMIILQKIA
                     DKKQLKWLDAFAMPISMFAAMGVVILVSQLLPPEIAFLEWRG"
     gene            complement(4962..5690)
                     /gene="fabG"
                     /locus_tag="PLD98_02380"
     CDS             complement(4962..5690)
                     /gene="fabG"
                     /locus_tag="PLD98_02380"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HQA05129.1"
                     /translation="MRTALVTGGGRGIGAAVARALCHEGYAVAVNCNKSYDEALRLSA
                     ELNSCGFVTKVFRCDVTSPDEVKRMFEDIERELSPVSVLVNNAGAAQQKLFCDITDKD
                     FQDILSVNLTGAFNCIREALPYMIREKKGSIINIASMWGQVGASCEVHYSAAKAGLIG
                     LTKALAKEVAPSGIRVNCVSPGAIFTDMLPQDKETIDRLKEEIPLGYIGSPEDVANLV
                     AFLASEKASYITGQVIGVNGGMVI"
     gene            complement(5707..6627)
                     /locus_tag="PLD98_02385"
     CDS             complement(5707..6627)
                     /locus_tag="PLD98_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05130.1"
                     /translation="MKKAITVFCIVLMALSVVFLVSCAKDKNDDIVRTTIPRTTLPGE
                     DETDESDEYIIVTNENGETVTDEAGQPVTEIPSKQQDPSDPDSGQQPGETTTKKSDAD
                     TPNQGGNNNGNNGGNNGGGLDQGPKAVRDAISILQSGRFYMKGSAYDGKETTPMKVAI
                     SGKNMYMETTMQGMNIAMLTLDGTIYLINPSKGAYWKPAKAFLKQMGMDELTPPDFGS
                     TTDKSKYKGSEEGVLNGKKMTCYVYEDTEGYSKWYTDSSGKVVAFEAYKTDGKLDSRM
                     TFEELSAAIPDGLLDNPEKRYKKGSINIFF"
     gene            complement(6781..7994)
                     /locus_tag="PLD98_02390"
                     /pseudo
     CDS             complement(6781..7994)
                     /locus_tag="PLD98_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059901.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
     gene            complement(8068..9309)
                     /locus_tag="PLD98_02395"
     CDS             complement(8068..9309)
                     /locus_tag="PLD98_02395"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HQA05131.1"
                     /translation="MKVAIMGFGVVGSGVAELFAKNHNKIVSSSTQPELELKYILDIK
                     DFPGSPFADMMIKDFNIILNDPEVGIVVETMGGLKPAYDYVSACLKAKKSVVTSNKEL
                     VAEKGYELLSLAKENGVNFLFEASVGGGIPIIRPISQCLAANEIDEIAGILNGTTNFI
                     LTKMFKEGMSFGDALKLAQENGYAESNPAADIEGTDACRKICILASLAFGKHVYPEEV
                     YTEGITKITTEDVDYAKNWGGVVKLIARAGRLENGKIFALVSPAFVKSSSQLASVDDV
                     FNAILVRGDATGDVVFYGRGAGKMPTASAVVADVIDCAKHAVSKKVFGWEDGIPNYVV
                     DYTSLPVSLYVRVSAVGNTSEIKKELESKIPGINYLVRSASPANEFAFVTPEMPENEI
                     REKLTEINGISIINTIRITDY"
     gene            complement(9598..10581)
                     /locus_tag="PLD98_02400"
     CDS             complement(9598..10581)
                     /locus_tag="PLD98_02400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQA05132.1"
                     /translation="MSDILYIVVPCYNEEEVLQETASRLRHKLASLIESGAVSEKSRI
                     LFVNDGSKDRTFEIIESLHKTCKLFAGLSLSRNRGHQNALLAGLLTAKNYADAVISMD
                     ADLQDDIDAVDEMIKKYKEGCDIVYGVRSKRKTDSFLKRFTAEGFYRLMNFLGANTIY
                     NHADYRLMSKRALEALENYKEVNLFLRGIVPLIGFKTDIVYYERHERFAGESKYPLKK
                     MLAFAFEGITSFSVKPIRFITMLGFFITLVSVGMFVYTLVRYFQGNTEVGWASLIISL
                     WAIGGLTLLSIGIIGEYIGKIYLEVKGRPRFIIDKLLLDEESEVKAEESSK"
     gene            10776..11045
                     /locus_tag="PLD98_02405"
     CDS             10776..11045
                     /locus_tag="PLD98_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQA05133.1"
                     /translation="MRAIVTVIGHDRTGIIAEISMLLFKAGVNILDINQTVMQEFFTM
                     TMLVDTKDATISFEELSKRLADKGEEMKLNIRIQREDIFNAMHRI"
     gene            11105..12463
                     /locus_tag="PLD98_02410"
     CDS             11105..12463
                     /locus_tag="PLD98_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HQA05134.1"
                     /translation="MLTLTDILQTIEMIDRQHLDIRTITMGISLCDCADESSKKAAKK
                     IYDKITRCAENLVSTGEAIEREYGIPIVNKRIAVTPMAIVAASCKDGNITDYAFALDN
                     AAKECGVNFIGGYSALVHKGMTEADKRLIESIPQVLSQTNLVCSSVNVATTKAGINMD
                     AVALMGSVIKKTAELTADRDSLGCAKLVVFANAVEDNPFMAGAFHGVGEPECVINVGV
                     SGPGVVKAALQECKGKSFDVVAETIKKTAFRITRMGQLVAMEASQRLNVPFGVVDLSL
                     APTPARGDSVAEILEEIGVEYAGAHGTTAALAMLNDAVKKGGVMASSHVGGLSGAFIP
                     VSEDEGMIRAAKAGALTIDKLEAMTCVCSVGLDMIAVPGDTPASTLAAIIADEAAIGV
                     INNKTTAVRIIPAVGKKVGDIIELGGLLGSAPIMPVNSYSSERFAARGGRIPAPMHSL
                     KN"
     gene            complement(12866..13567)
                     /locus_tag="PLD98_02415"
     CDS             complement(12866..13567)
                     /locus_tag="PLD98_02415"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HQA05135.1"
                     /translation="MSYCVNCGVELDSSAKICALCSTPVINPNIKTTEPAPVPYPDKY
                     MIPPKVRRRYAAFLATMIMLIPNIVCVLVNLLVPRGGKWSIFVVASSALLWMMVFLPM
                     LMKKTRPFILLTVDAVALCLYIYVFYAIYRQEGWFTGLVFPLIAGLYVIIAGMIKWLR
                     KKRDWVKIAINVFLSIAVYSLYADFFISRFYEAERPLGVSLVIAASCIALIIFFKFVE
                     RNKRFRAWLTRKFFV"
     gene            complement(13589..14944)
                     /locus_tag="PLD98_02420"
     CDS             complement(13589..14944)
                     /locus_tag="PLD98_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05136.1"
                     /translation="MAEKEQKENHGGKFWLKLDNAGKVFPGQNTQTWSNIFRGAITLK
                     EKVDPAVLEEALARVLPRFPCFDVNIKRGFFWYYFEKAKYPAPPVMPDVGNPCHRIKF
                     RENKGFLFRVYYHERRIAVEFFHALTDGYGGSLFLCTLTAEYLRIKYGANIPPGGAVL
                     SLQETADEFELEDSFERFASSKVKAKRRDRFVYHKKGERLPAHMVNVTTGYMPVDKLL
                     ELARANNASITEYLAAILMYIHYNIQKEEERKQKDVSVQIPVNLRRTFPSKTLRNFSL
                     CYSVRINPNMGEYTFEEILKHISLYLRFINNEKTLNSMMTANMALERNPFMRALPLPV
                     KWLAIAISFGLTAEKTTTAMITNLGPVKVPDEMLEYVESYVMMPSPGKLNGARVGAAS
                     IGNTFAATFANIYTDSRIEREFFTFLVKKGIPVKIESNRESIPENFKNNPYAFNPQIT
                     K"
     gene            complement(14958..16007)
                     /gene="ispG"
                     /locus_tag="PLD98_02425"
                     /gene_synonym="gcpE"
     CDS             complement(14958..16007)
                     /gene="ispG"
                     /locus_tag="PLD98_02425"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQA05137.1"
                     /translation="MERRKTRQVAAGSVPIGGGAPIAVQSMLNKPAHDIEGNVEQAKT
                     LEKAGCEIIRLSVPDKEAVKTLYAVKQSVSVPVVADIHFDYRLALESAAAGADKIRIN
                     PGNIGSDENVAAVARECKTRGIPIRIGVNSGSVEKHILKKHGGPTPEALVESALYHVS
                     LLEKCDFYDIVLSMKSSDVVSTVRAYELASERCGYPLHIGVTEAGTERTGIIKSSAAL
                     GALLLEGIGDTIRISLTADPVNEVAAGIALLKSLGLRKQGVNIISCPTCGRTKIDLIK
                     LANEIENRLSNLDKNISVAVMGCVVNGPGEASHADIGIAGGDGCAVIFKGGKVLKKVS
                     EDNIIEELMAEIEKL"
     gene            complement(16023..17108)
                     /locus_tag="PLD98_02430"
     CDS             complement(16023..17108)
                     /locus_tag="PLD98_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HQA05138.1"
                     /translation="MIFPLSIAELWSKAWPILFAIFFFGLVITVHEVGHFIFAKLFNV
                     KVNQFAIGMGPAIFKKKKGDTLYAVRLLPMGGYVNMEGEDEESQHERSFGKKPIWQRF
                     IIVSAGAIFNLITGLVIVAIMLGQQNLIGTTQIRHFANNAKSHETGLEPGDRIVRIDG
                     MKIYSDSDISYALLRSTGPTYTFVVDRNGEEIKIEGVEFDRQPSPAGKGDIVRIDFVL
                     VGEKVTFGRVFKNAIIETASMARIVRLTIVDLIRGRYGINDLAGPIGTINIIAGAASE
                     AAASKGQDLTGILSILAFISINLGIFNLMPIPALDGGRLFFFIVEAIRRKPIPQKYEA
                     LVHAVGFGLLIGLMVVVSINDIFGVFK"
     gene            complement(17262..18419)
                     /locus_tag="PLD98_02435"
     CDS             complement(17262..18419)
                     /locus_tag="PLD98_02435"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQA05139.1"
                     /translation="MNSLIILGSTGSIGAQALDVAEKLSLKVEALTANSDAKKLEEQA
                     RKFKPRLAALADEKAAAELKIKLADTNTKVLSGIEGVTECAAFGENPTVLNSIVGMAG
                     LRPTLAAAKAGRRIALANKESLVAGGKLVKTAVKENNAELLPVDSEHSAIFQSIQGCK
                     DKKEIKKIILTASGGPFFGKKRHELKSVTLQDALKHPNWSMGAKITVDSATMFNKGLE
                     LIEAVWLFDVAPENIEIVVHRESIVHSLVEFADNSVIAQLGLPDMRIPIQYAITYPNR
                     FESPVGSLNLAQIGKLTFFEPDYETFKCINICRDAINRGGLYPAAANAANEEANELFR
                     KGKIPFLAIGDIIEEALLDIENKEEYRLEDVEEISDAARERVRRLSEKYTL"
     gene            complement(18434..>18806)
                     /locus_tag="PLD98_02440"
     CDS             complement(18434..>18806)
                     /locus_tag="PLD98_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HQA05140.1"
                     /translation="FAYFVGVKFGKHKMSPNISPKKSIEGAVGGVIGAIILNLILCAV
                     FKNCFPGKSTMMSYFVVAPLSAVLSLTSMLGDLSASVIKRNFGVKDYGNILPGHGGVM
                     DRFDSVIFVMPVLYAFVMLWV"
BASE COUNT         4569 a         4575 c         4534 g         5128 t
ORIGIN      
        1 tgccgtcaat ggttatctcg ccctgaccgt caaggcagat tactatagcg tctccgtcgg
       61 aggcgtgggt gctgatttcc tctcccttat caaaggcaaa gagggttacg ctgttgtact
      121 gattctgagc caaagtcttg ctgactatct gtcccggctg gtatgatacc tcatccaaaa
      181 gggacagagg ggtttcgtgg gcgatgtttt taatgtactt ttccatagcc tttctcctat
      241 tcgtctataa tttcgccgtc tgtggagatg gtcacgacct gccacggaat catctttccg
      301 ctgtttctga gggctgtgat ggcggcgttc gcaattcccg cgcagcaggg gacctccatc
      361 ctgacaatgg taatgctttt tatatcattg tttcttatta ttgcctcaag cttctgagaa
      421 taatcaacgg tatcaagctt cgggcagccg attattgtga tgtgattctt tataaagcgc
      481 tcgtggaagt caccgtatgc aaaggctgtg cagtcggcgg cgataaggag cttagcgccg
      541 ttaaagtagg gagcgtttac ggggacaagc tgaatctgga cgggccactg catgagcctg
      601 ctctctctgg gggcggcggg ggcgttctga gcattgacgc tttcctcgcc cctttcaagc
      661 gtcttggact gtgagccggg gcagccgcag gggaggttat cttccttttc ttcccttaca
      721 atcgcccttg ccatgctgcc ggggcagccg ccgaacatcg gctttctttc gggagttttc
      781 ttttcttctg tatcagcgtt cttgccttcg gtctttgcct ttaccgcctc ctcgtcaaag
      841 ggcagagcct ctctcgtttc aaaggagatg gctcccgtgg ggcatacggg caggcagttt
      901 cccaaaccgt cgcagtagtc gtctctaaga agttttgcct ttccgtcaac tatgccgatt
      961 gcgccctcgt ggcaggcctg cgcgcagagt ccgcagccgt tgcaaagttc ttcatctatc
     1021 ttaataatct gtcttaccat atctaatacc tctctctatt tttatattaa atttcttgac
     1081 cttgcaagtt taatttacta taatcaaaca aaagagtccg ttgtttttac aacgaaatga
     1141 ggtttttatg aaagaatata ttgatttgtt atccaagaac cctcttttta agggcataga
     1201 caagggggat atcggttttc tgctggactg cctttcggca aaaaccaaaa catattataa
     1261 agacgaaccg atttttgagg cgggctcaag acccgaatat gtggggatag tgctctcagg
     1321 ctcagcgttt gttttgcagg aggattactg gggcaaccga aatatacttt cgaatatcgg
     1381 cgtgggcggt attttcggcg aggccttttc ctgcgcggag gctgctgccc tgcccgtatc
     1441 agttatcgca aaagaggatt cggtaatact ccttataaat gttaaaaaga ttttgactat
     1501 ctgctccacc gcctgcgctt ttcacacttc gctcataatg aaccttatga gaatactcgc
     1561 gaacaagaat agatacctta cccgtaaaat tgagcacata acaaaaaaga ccacaaggga
     1621 aaaggtgctg tcttttttgt ctgagtgcgc cgtccagggc gggaagaata cttttgagat
     1681 tgagtttaac aggcaggagc tggcggatta tctctccgtt gagcgcagcg ccctgtccgc
     1741 cgttttaagc aagatgaaag aggaaggcat catagactat tacaaaaata ccttccgcct
     1801 tacggataaa aaataaaaac ctcagctttg gctgaggcct ttttatgctt tagcgtcatc
     1861 catgtcttta aggtgcagct ttaataccgc gttgtatgtc ttaatctgct ccaatattct
     1921 gaaaaccttg gactccaatt cgctgccttc gctgtagtcc gcgggggcga ccaaatccac
     1981 cctgccctgc tctatcagct cgtttacaat ctttctgtca tcgttctggt aaaccgctat
     2041 tgagtgaccg ccgtttacct tgcacagctt catgcatggc acatcggtgt agccgtcgcc
     2101 tatgtatatc atatttttaa aggggaccct tcgcttgctg tcggtggtgt agcggttcag
     2161 actgtcgtgg tcggtgacat ccagaacgcc cttgtttatg cggtacaaaa actgggtttt
     2221 actggtatag tttaccgcca tggcgggcca aatgggcacc tgctcctcgt catagcagaa
     2281 ctcggcggcg taaatccttt taaagtgctt tgctatcttt gtgccctcga taatctcttt
     2341 aagccctgat gagattatgt agtgttcaac cgtaagtccc aaactctcgc caaaactgtt
     2401 gaccctctcg aaccatgttt caactccgtc aaagaaatct acactctccc ccagtttcct
     2461 gaaaatctcc cttgtgatga gcattttgcc gcggctggac tttaccatgt agtacatata
     2521 ggcaagaatc gggtccatgt tgttagccgt ttcatactcc ttgcaaaggc gccaaaactc
     2581 atcggcagtc ttgccgatgc cgggaataaa ggcgtaatcc tgcatattgc cgggagagag
     2641 ggttttgtca aagtcataca ttatggcgac tataggcttt gagttcaagg tttttccccc
     2701 taattgcttt ttaacaattg tagcatattt gggcggcata aaaaaggtcc cataaaatat
     2761 aaaaaaccgc cgtttggcgg ctttttatat taaaactaat taggcaaaca gatcataaac
     2821 tcttttgaag atctcgagta atcccttaat gaaatcagcg atgattgcaa aatcgaacat
     2881 ccttttgcac ctcactttca aatctagatg gatgcgtcag cttaaaagtt ttcttcaggc
     2941 tttataatta ctcttcaata cccttaagct tgttaaggaa ctccttaaga agtgcgaaga
     3001 aatcaacgat agcctgcatc aatgcatcaa aatcaaagct catgcctaca cctccttaaa
     3061 tgtattaccc ttttggcgga tggataaaat ccccaccaat tcttacaact agagattagc
     3121 acaagcaatt ttaaaattca atagaattgc gccaaagttg taaaagttta caagtatgca
     3181 aacatcatgt aaaatttgtt gcattttgat aatattttta cccttttatc ttgcttattt
     3241 ggaaaaaagt cagattacta atccatttcc tacatgaaaa tagtcgtaaa ttgacaaagg
     3301 gctttgagat tgttgcgaaa atattgcaga tgtatgtcct gctatataag attcttttta
     3361 tacattaaat gggctgcgag aatcgctacc acgactccga tggggataag aattcccacc
     3421 gacatctgtc ccgcaaagat gagaattatc agtatgaccg caaggggggc tacgaccgtc
     3481 ttggggtact taagcagata cgctgtcagg atggagccga acagcgcggg cagaacctga
     3541 gagaaggcgg gagcgaaaat agagctttcg tccgtgatta tggggataat gggtctcagg
     3601 aaaaatacgc agaccgctac tattactatt gtggcaatcg atgaggctgc gattgagatt
     3661 gtggaaatta tctcgccctc ctcgctgttg ggctttacct tagcggcgtt catggcagct
     3721 atggcgcagg ggagcttgag gttagatatg tcgcccgtaa tgaagctgag atacatgccg
     3781 cccgttccca gcagcggcgc gtaagtcaga acctccacaa cagcggtggg atagtagaac
     3841 atggctatgg ggagaagtcc ctttaaaacc gcggcgggct ttggccaagc gttgtagtaa
     3901 acgcacatgg agagaggaac gcctataaat actataagaa tgaaaaccat ccacagcctg
     3961 ccgtaaaaat ggatggactc gttgtaatcc tttacttttt tcatttcagc tcactgcctt
     4021 tctttaaaat cttggtttct ttcatcttta acccctccac tcaaggaacg caatctcggg
     4081 cggcaggagc tgtgaaacca gaatcaccac gcccatggcg gcaaacatgc ttataggcat
     4141 agcgaaggcg tcaagccatt tgagctgctt tttgtcggcg attttttgaa ggataatcat
     4201 aaagagaacc gagaaaacaa gggctgtgac ggagagtacg cccgcgccgt cggttaagac
     4261 ctctgggctt acatccacat tgccttgacc cagtatagct ctgcccacaa aggcacagat
     4321 aagacccaca aaggcggcgg aggaaatgac atccgcccat tttgtgtcct tagagagagc
     4381 cttgcccatc ttcttttgaa tccttttaag gaaaatcggg ataattacaa gaggcagcat
     4441 actgccgagg gtcataaccc atgctatggc gctgaaaacc tcggggtctg tgacctcgct
     4501 tgtaagtccg cccagtccgt acgcctccat ggcggcggag gcagcggcgg tttcaaagct
     4561 gatatttcct ataataagcg ttctgaccca agggagtacc aatcccaagg ctcccgcaag
     4621 ggcgaccacc gtcgccacta ttgaaagagc gggagttatg gagaacatta cgcttgtaac
     4681 taccgtgttt ttcatgtcct ttaccgtaat gcccagctcc ttgccccttc tccatgcctt
     4741 gacaaggaag aaaacagcct gagccacaac aattacggca acaaccaagc ccatggcata
     4801 cataaagcca gcttctttaa aatctgtcat ctcgcacctc gctgcgccgc cttatcaggc
     4861 ggctgagttt agaatacttt taaataatat cacgacataa gttcgttagt caattaacaa
     4921 aaacaaaccc catacagtat ttgctgtatg gggtttgttt attaaattac cattccgccg
     4981 tttacgccta ttacctgtcc cgttatatag gacgcctttt ccgaggcaag gaaggcgacc
     5041 agatttgcca catcctcggg actgcctata taccccagcg ggatttcctc ttttagtctg
     5101 tcaattgttt ccttatcctg cgggagcata tcggtgaaaa ttgctccggg ggacacgcag
     5161 ttgaccctga tgccgctcgg cgccacctct ttcgccagag cctttgtaag accgataagc
     5221 cccgccttcg cggcggaata atgcacctcg caggaggctc ccacctgccc ccacatggag
     5281 gcgatgttta ttatggagcc tttcttttcc cttatcatat agggcaaagc ctctctgatg
     5341 cagttaaagg ctccagtaag gtttacggat aggatgtcct gaaaatcctt gtcggtaatg
     5401 tcgcagaaaa gcttctgctg agccgctccc gcgttgttca caagcacgga gacgggggat
     5461 agctcccttt caatatcttc aaacatccgc tttacctcat cgggggaggt gacatcgcag
     5521 cggaaaacct tcgtcacaaa accgcaggaa ttaagctctg cggacaagcg cagagcctca
     5581 tcgtaagatt tattgcaatt gaccgcgacg gcatagcctt catggcacaa agcgcgcgcc
     5641 actgccgcgc ctatgcctct gccgccgccc gtaacaagtg ctgtcctcat aagccatttt
     5701 ccttcattag aagaagatgt ttatgctgcc ctttttgtat cttttttcgg ggttatcaag
     5761 cagaccgtcg ggaattgctg cgctcagctc ctcaaaggtc attcttgagt caagcttacc
     5821 gtccgtttta taggcttcaa aggcgacaac cttgcctgag gagtcggtgt accacttgct
     5881 gtagccctct gtatcctcat aaacatagca tgtcatcttt ttgccgttaa gtacgccttc
     5941 ctcagagccc ttatacttgc tcttgtcggt tgtgctgccg aaatcgggcg gggtaagctc
     6001 gtccatgccc atttgtttaa ggaaagcttt tgcgggcttc cagtaagctc cctttgaggg
     6061 attgataaga tatatggtgc cgtcaagggt cagcatggct atattcatgc cctgcatggt
     6121 ggtttccata tacatattct tgccggagat ggcaaccttc atgggagtgg tttctttgcc
     6181 gtcataggca ctgcccttca tgtagaacct gccgctctgg agaatagaga tggcgtccct
     6241 tacagccttg ggaccctgat ctaagccgcc gccattgttg ccgccgttat tgccgttgtt
     6301 attgccgcct tgattcggcg tatcggcatc tgactttttg gttgtggttt caccgggctg
     6361 ctgaccggag tcggggtcgg aggggtcctg ctgtttagaa gggatttcgg ttacgggctg
     6421 acctgcctcg tctgtaacgg tctcgccgtt ttcgtttgtt actataatat attcgtcgga
     6481 ctcgtccgtc tcgtcctcac cgggaagggt ggtacggggg atggtggtcc tgacaatatc
     6541 atcatttttg tcttttgcgc aggataccag aaaaacaacg ctgagagcca tcagtacgat
     6601 gcagaaaact gtgattgctt ttttcatttt cctcattccc aatttcttat tctgtgcaaa
     6661 gctcacaata ttagattaca ctaaactgac aaaattagca agagtttttt accccgcaga
     6721 aaatgccagc gctttcggga cacaaaaaaa ggcaggctta gcctgccttt cagtatttaa
     6781 ttattcaacg ggaggcagaa actttgcgtg gattgcggct actgcctcgt cggcgtattc
     6841 ggcgtcgata agaaccgaaa tcttaatctc gctggttgaa atcatctgga tgttgatgtt
     6901 tctctcataa agagcctcga acatcttggc tgccgcgccg gggtgggtct ccatgcccgc
     6961 gccgactacc gatacctttg cgattcctgt atcaagcttt acattgtcgg ggttgatttc
     7021 ggctattgaa gtcttgagaa cctcaattgc aaggtcagca ttatccttgg atactgtgaa
     7081 ggctatgtcc tttgtgccct ctctgccgac cgactgaagg ataatgtcca cattgatatt
     7141 cttagctgcc agcttgctga agattttgaa tgctataccg ggaacatcgg gaacctcggt
     7201 aatagaaatg cgggcaacat ttacatcttt tgtaacgcct ctgacaagca ttttttccat
     7261 attaacaacc tccttgacca ttgtacccgg ctgccttgtt ggcttgagag cacctctaat
     7321 tcaacattgt atttcttagc catttccacg gaacggttat tgagaacctg cgcgccaagt
     7381 gtcgccagct cgagcatttc gtcgtatgtt atttcgtcga gcttaattgc ggcttttacc
     7441 tttctcgggt cggctgtgta gacgccgtca acatcggtga aaatctggca cttatcagca
     7501 tggagggctg ccgccaacgc taccgcgctg gtgtcggagc cgcctctgcc gagggtggtg
     7561 acatcgtcgt acttattgat tccctggaaa cctgccacga taacaatatt gcccttggca
     7621 agctctgctt tgattctggt tgtgttgacg ctcttgattc ttgctgagcc gtaatccgag
     7681 ctggtgagga ctcctgcctg ccatccaagc aaggaaacag cgggatagcc catttcccta
     7741 attgccatag caaccagggc tatggagatt tgctctccgg aggctaaaag catatccatc
     7801 tctctcttgg gaggcttagc cgaaatctct ttagccttgt cgataagctc gtcagtggtg
     7861 tcgccctggg cggaaacgac tacgacgact tcgtttccgg ccttgtaagt gtccgtaatg
     7921 atgctggcaa ctttcctgac tctatcggca ttagcaacgg agcttccgcc gaatttctga
     7981 acaatcagtg ccataatact cctccattat ttaatccgag cttttctcgg aatagactgt
     8041 cttactaaat atatgatatt cgcctcatta ataatcggta attctgattg tatttattat
     8101 gctaataccg tttatttctg tgagcttctc tctgatttca ttctcgggca tctcgggggt
     8161 tacgaacgcg aactcgtttg cgggagaggc gcttctgaca agataattta tgccgggaat
     8221 cttgctttca agctccttct ttatctcaga agtgttgccc accgcgctga ctctgacata
     8281 aagggatacg gggaggctgg tgtagtcaac aacataattc gggatgccgt cctcccagcc
     8341 gaaaaccttc tttgacacgg cgtgctttgc gcagtcgatg acatcggcaa caacggcgct
     8401 ggctgtgggc attttgcccg ctcccctgcc gtagaatacc acatctcctg tcgcgtcgcc
     8461 tcttacgaga atggcgttga acacatcgtc aacggaggcg agctggcttg agctcttaac
     8521 aaaggcgggg cttacaaggg caaagatttt gccgttttcg agccttccgg ctctggcgat
     8581 gagcttcacg acgccgcccc agttctttgc gtaatccaca tcttcggttg tgattttggt
     8641 aatgccctct gtgtagactt cttcggggta aacatgcttt ccgaaggcga gggacgcgag
     8701 tatgcagatt tttctgcagg cgtcggtgcc ctctatatcg gccgcggggt tgctctccgc
     8761 atagccgttt tcctgagcga gcttcagcgc gtcgccaaag ctcatgccct ctttaaacat
     8821 ctttgtgagt ataaagtttg tggttccgtt gagtatgccc gctatctcgt caatctcgtt
     8881 agcggcaagg cactggctta tggggcggat aatcggaatg ccgccgccga cgcttgcctc
     8941 aaaaaggaag tttacgccgt tctctttggc aaggcttaaa agctcatagc ccttttcggc
     9001 gacaagctct ttgtttgagg ttacaacgct cttttttgcc ttaaggcagg cgctaacata
     9061 gtcataggca ggctttagtc cgcccatggt ttcaaccact atgcccactt cggggtcatt
     9121 gaggataatg ttgaaatctt ttatcatcat atcggcaaag ggtgagccgg ggaaatcctt
     9181 tatatccagg atatacttca gctcaagctc aggctgggtg ctgcttgata ctatcttgtt
     9241 gtgatttttg gcaaacagct cagcgacgcc cgagccaact acgccgaaac ccataatggc
     9301 gactttcatt tttcatcccc ctacacacag agtgtttata taatgagagt atattctgaa
     9361 taaaaatgca aacagcccga aggccgaatt ccataaattt tggcaccaag acacgaattt
     9421 aaataatata atagcataaa agccttaacg aataaagtat tttttatgat ttgtgaaaaa
     9481 tgcatatata agcaggaaag cgaataagtc ttctggatat ttaatccaag atacaaaatg
     9541 agcggcactt taaataaatg ccgccccgat tttttcctat gccgtttttt ctttaagtta
     9601 ctttgagctc tcttctgctt ttacttcgct ttcctcatcc agaaggagct tgtctatgat
     9661 aaagcggggt ctgcccttta cttcaagata tatctttcct atatactcgc cgattatgcc
     9721 gattgaaagg agcgtcagcc cgccgatagc ccaaaggctt atgataaggc tcgcccagcc
     9781 aacctcggta ttgccctgaa agtatcttac aagggtataa acgaacatgc ccacgctgac
     9841 caaagtgatg aaaaagccca gcatggtgat gaatcttatc ggttttaccg aaaatgaggt
     9901 tatgccctca aaagcgaagg caagcatctt ctttaagggg tacttgctct cgcccgcaaa
     9961 gcgttcgtgt ctttcatagt aaacaatatc ggtcttaaag ccgataagcg gcactatgcc
    10021 ccttaaaaag aggttgactt ccttgtagtt ttcgagagcc tccaaggctc ttttgctcat
    10081 gagtctgtag tcggcgtggt tatatatagt attcgcgccg aggaaattca taaggcggta
    10141 aaagccctcg gcggtaaatc gctttaaaaa gctgtcggtc tttcttttgg agcggacacc
    10201 gtagacaatg tcgcagccct ctttgtactt tttaatcatt tcatcaacag cgtctatgtc
    10261 atcctgcaaa tccgcgtcca tggaaatgac cgcgtctgcg taatttttcg ccgtcaaaag
    10321 tcccgcaagc agggcgttct gatgccctct gttgcggctt aaagaaagtc ccgcaaagag
    10381 cttgcaggtt ttgtgcaggc tctcaataat ctcaaaagtt ctgtcctttg agccgtcgtt
    10441 tacaaacagg attcggctct tttcggaaac ggctccgctt tctataaggc ttgcgagctt
    10501 gtgccgaagc cttgaggcag tttcctggag aacctcctcc tcgttgtagc agggcacgac
    10561 tatgtataat atatcggaca tttttctctc tcctttaaga ttagataagg acaccttaat
    10621 ctaatcaact gtaataatat cataagcaga acggcttttc aacttaaagc ggcgcggcaa
    10681 atactcaaag tttctacatg gtttaattgc gctaaagtct taattgtgat ataataattt
    10741 tctgaatttg aatttccctt ttcttggagg atgacatgag agctattgta accgttatag
    10801 gtcacgacag aacgggcatt atcgcggaaa tcagcatgct gcttttcaaa gcgggcgtga
    10861 acattcttga catcaatcag acggtaatgc aggagttctt tacaatgacg atgctggttg
    10921 acacaaaaga cgccacaatc agctttgaag aactaagcaa gcgtcttgcc gacaagggcg
    10981 aggagatgaa gctgaatata agaattcagc gggaggacat cttcaacgcc atgcacagga
    11041 tttagtctac aattagactg ttaaactctc tattttgttc aataaatttt tgtaggggaa
    11101 aaatatgctg actcttaccg atattcttca gacaattgaa atgattgacc gccagcacct
    11161 ggacattcgc accatcacaa tgggaatctc cctgtgcgac tgcgccgacg agtcatcaaa
    11221 gaaggcggct aagaagattt acgataaaat cacccgctgc gccgaaaacc ttgtaagcac
    11281 gggcgaggca atagaaaggg aatacggcat tccgattgta aacaagagaa tcgccgtaac
    11341 tcccatggct atcgttgccg cgtcatgcaa ggacgggaac ataacggact atgcctttgc
    11401 ccttgacaac gccgcaaaag agtgcggggt aaacttcatc ggcggctatt cggcgcttgt
    11461 tcataaagga atgaccgaag ccgacaaaag gctaatcgag tccatcccgc aggttctgtc
    11521 ccagacgaat ctcgtttgct ccagcgttaa tgtcgccacc acaaaggcgg gcattaatat
    11581 ggacgcggta gctctcatgg gctccgttat aaaaaagacg gcggagctga ccgccgaccg
    11641 tgacagcctg ggctgcgcaa agctggttgt tttcgccaac gccgttgagg ataacccctt
    11701 tatggcgggc gccttccacg gagtcggcga gcccgaatgc gttataaatg ttggcgtcag
    11761 cggccccgga gtcgtaaagg cggctctgca ggagtgcaag ggcaagagct ttgatgtggt
    11821 cgccgaaact attaagaaaa ccgcgttcag aataacccgc atgggtcagc ttgtggctat
    11881 ggaggcttcc caaaggctta atgttccctt cggcgttgtg gacctctccc ttgcccccac
    11941 acccgcaagg ggcgacagcg tagcggaaat ccttgaggaa atcggcgttg aatacgccgg
    12001 agcccacggc acaacagccg cacttgccat gctcaacgac gctgtcaaaa agggcggagt
    12061 tatggcaagc agccatgtgg gcggactttc gggagccttt attcccgtca gcgaggatga
    12121 gggcatgata agagccgcaa aggcgggcgc cctcacaatt gacaagctgg aggcaatgac
    12181 ctgcgtatgc tcagtgggcc ttgacatgat agccgttccc ggcgacaccc ccgcaagcac
    12241 tctggcggct ataatagccg acgaggcggc gataggcgtt atcaacaaca agaccaccgc
    12301 cgttaggatt atccccgctg tgggcaagaa ggtcggcgac ataattgagc tcggcggact
    12361 tctcggcagc gcgccgatta tgccggtaaa ctcatattcg agcgagagat tcgccgcaag
    12421 aggcggcaga atccctgccc cgatgcacag tcttaaaaac taaacacaca gagccttgcg
    12481 agtcgcaagg ctcttttttt ggagatagcc aaagtttcga agctcaattt tgtctattat
    12541 gttattaaac tgttatcttt ctttgaacgg atgcataatt cacagaatct ttataaatat
    12601 tttacaaaaa agacccataa aaatagtgca aattgttgac ttaagtactc cgggatgata
    12661 ccattaaggt aagctaaagg actgtttatt gggtggtaga gtggagattt gctttctgat
    12721 tcacagtctt aaggctaaaa agagatcgct tcgatgaact ccttttttgg gacatatccg
    12781 aacggatatg tcccatcgtt cttaaagcgc gcacagtccc cttttaaaaa actaaagcct
    12841 gcggttattc cgcaggctct ttttattata caaagaattt tctggtaagc caggctctga
    12901 agcgtttatt cctttcaaca aacttaaaga aaatgataag ggctatgcat gaggcggcta
    12961 tgacgaggga tactccgaga ggccgctcag cctcataaaa gcggctgatg aagaaatcgg
    13021 cataaaggct gtaaacggct atgcttaaaa agacattgat tgcgattttg acccagtctc
    13081 tttttttccg cagccatttt atcatgcccg cgattatcac atagagcccc gctataagag
    13141 gaaagacaag ccccgtaaac cagccctcct gcctgtatat ggcgtaaaag acatagatgt
    13201 ataaacaaag ggcgaccgcg tccacggtca gcaatataaa ggggcgtgtc tttttcatca
    13261 gcatggggag gaaaaccatc atccataaaa gagctgagct tgccactaca aagattgacc
    13321 atttgccgcc ccttggaaca aggagattta caagaacgca gacgatgttt ggtatgagca
    13381 ttatcattgt ggcgaggaac gcagcgtacc tgcggcgaac cttggggggt atcatgtatt
    13441 tgtcgggata gggaacgggg gcgggctctg ttgttttaat attggggttt attacaggcg
    13501 tggagcaaag ggcgcatatt ttcgcgctgc tgtcaagctc aacgccgcag tttacacaat
    13561 aggacatatg accacttccg accgattgct attttgtgat ttgaggatta aacgcatagg
    13621 ggttgttttt gaagttttcg ggaattgact ccctgttgct ctctatctta acgggaatgc
    13681 ccttctttac aaggaaggta aagaactccc gctcaattct gctgtctgtg tagatattgg
    13741 cgaaggtggc ggcaaaggtg ttgccgatgg acgccgctcc cactctcgct ccgttcagct
    13801 tgccggggga gggcatcata acatagctct ccacatattc aagcatttcg tcgggcactt
    13861 tgacgggacc caaattcgtt atcatcgctg tcgtggtctt ttcggcggtt agaccgaagg
    13921 atatcgcgat ggcaagccac tttacgggaa ggggcagggc gcgcatgaag gggttgcgct
    13981 caagcgccat gttggcggtc atcattgaat tgagggtctt ttcattattg ataaagcgaa
    14041 ggtacaggga gatatgcttt aagatttcct caaatgtgta ttcccccatg ttgggattga
    14101 ttctgaccga gtagcaaagt gaaaagtttc tgagggtttt ggatgggaaa gtgcgcctta
    14161 ggtttaccgg tatttggacg cttacatcct tctgttttct ttcctcttcc ttttggatat
    14221 tgtagtgaat atacatcagt attgccgcaa ggtactcggt tatggacgca ttattcgcgc
    14281 gggcgagctc caaaagcttg tccaccggca tatagccagt agtgacattg accatgtggg
    14341 cgggaagtct ttcgcccttt ttgtgataga cgaatctgtc ccttcttttt gcctttacct
    14401 ttgaggaggc aaaacgctca aaggagtcct caagctcgaa ctcgtcagca gtttcttgga
    14461 gtgaaagaac cgctccgcca ggggggatgt tggccccgta cttaattctt aaatactcgg
    14521 cggtcagggt acacaaaaac aggctgccgc cgtagccgtc ggtcagggcg tggaagaact
    14581 ccaccgcaat tctgcgctcg tgataataga ccctgaacaa aaagcctttg ttttccctaa
    14641 actttattct gtggcagggg ttgcccacat cgggcatgac gggcggggcg gggtactttg
    14701 ccttttcgaa atagtaccag aaaaagcccc ttttgatatt gacatcaaag cagggaaaac
    14761 gaggcagaac gcgcgcaagg gcttcctcaa ggacggcagg gtcaaccttt tcttttaagg
    14821 tgattgcgcc cctgaagata tttgaccaag tctgagtatt ctgaccgggg aaaaccttgc
    14881 ccgcgttgtc gagtttaagc cagaactttc cgccgtgatt ttccttttgt tccttttcag
    14941 ccatgttccg caccaaatta cagtttttct atttcagcca taagttcttc tatgatatta
    15001 tcttcgctaa cctttttaag gaccttgccg cctttaaaga taacggcgca gccgtcgccg
    15061 ccggctatgc cgatgtccgc gtgggacgcc tccccggggc cgttgacgac gcagcccata
    15121 acggcgacgc tgatattttt atcgaggttt gagagcctgt tctcaatctc atttgcgagc
    15181 tttatcaaat ctattttagt cctgccgcat gtggggcagg agataatatt cacgccttgt
    15241 tttctaagtc ccaggctttt aagcagagca atgcccgcgg cgacctcgtt tacggggtcc
    15301 gcggtaaggg agattctgat tgtgtcgcct ataccctcta acagaagggc gccaagggcg
    15361 gctgaggatt ttataattcc cgtgcgttcc gttccggcct cggtaacgcc tatatgtaag
    15421 gggtagccgc accgctcgga cgccagctca taggctctta cggtgctgac cacatcggag
    15481 gatttcatgg ataaaactat gtcgtaaaag tcacactttt caagcaggga gacatgatac
    15541 aaagcgcttt ccacaagcgc ctcaggagtg ggcccgccat gctttttaag gatatgcttt
    15601 tcaacggagc cggagtttac accgattctt atggggattc cccttgtttt gcactccctt
    15661 gctacggcgg cgacattttc gtcggagccg atattgccgg ggtttattct aatcttgtcc
    15721 gctcccgcgg cggcgctctc aagggcgaga cggtagtcga aatgtatgtc cgccacaacg
    15781 ggtacggaca cgctctgctt tacggcataa agggttttca ccgcctcctt gtcggggacg
    15841 gagagcctta ttatctcgca gcccgctttc tcgagagtct ttgcctgctc cacatttcct
    15901 tcaatatcat gggcgggctt attcagcatc gactgaacgg caatcggggc accgccgccg
    15961 attgggacgc tgcccgctgc tacctgcctt gtctttcgcc tttctatcat cttactcctg
    16021 ccttatttga atactccgaa aatatcgttt atagatacca caaccataag cccgataagc
    16081 agaccaaagc ccacagcgtg aacaagagcc tcgtacttct gcggtatggg ctttcttctt
    16141 attgcctcca caatgaagaa gaacagcctg ccgccgtcaa gggcgggtat gggcataagg
    16201 ttgaaaatgc cgaggtttat tgagataaac gcgaggattg agagtatgcc cgtaaggtcc
    16261 tgacctttgg atgccgctgc ctctgaggcg gcgcccgcga ttatattgat ggtgccgatg
    16321 ggacccgcaa ggtcatttat gccgtatctg cccctaatca agtccacaat ggtaaggcgc
    16381 acaatccttg ccatggatgc ggtttcgatt atggcgtttt taaatactct gccaaaggtg
    16441 accttttcgc ccacaaggac aaagtctatt ctgacaatat cccccttgcc ggcggggctg
    16501 ggctgacggt cgaactcaac gccctctatc tttatctcct cgccgtttct gtcaacgaca
    16561 aaggtatagg tggggcctgt gctgcgcagc aaggcgtagc ttatatcgct gtcggaataa
    16621 atcttcattc cgtcgattct cacaatcctg tcgccgggtt caagacccgt ttcgtggctc
    16681 tttgcgttat tggcaaagtg gcgaatctga gtagtgccga taaggttttg ctgacccagc
    16741 attatggcga cgattacaag acccgtgatg agattgaaaa tcgcgccggc ggagacgatt
    16801 ataaagcgct gccaaatggg ctttttgcca aagctccttt cgtgctggga ctcctcgtcc
    16861 tcgccctcca tattaacata gccgcccata ggaaggagtc gaacagcgta gagggtgtcg
    16921 ccttttttct ttttgaaaat ggcgggaccc atgcctatgg caaactggtt gaccttaaca
    16981 ttaaaaagct tcgcgaaaat gaaatgtcct acttcatgga cggtgattac aagaccgaaa
    17041 aagaagatgg caaaaagtat gggccatgct ttggaccaaa gctcagctat actaagggga
    17101 aaaatcaagg ttacagcacc tctttcacat ccgctaaagc ctcgtggaag atggaagaac
    17161 tactttaggg aaattttagt tggtatttta gtatatcacg ggtcttaatg atttaatgcc
    17221 cgtttagaaa aaatttataa actcttaaca ttaaactacg gttaaagagt atatttttcg
    17281 ctgagccttc tgaccctttc ccttgcggca tctgagattt cttccacatc ctcaaggcga
    17341 tattcctctt tattctcaat gtctaagaga gcttcctcaa ttatgtcgcc gattgctaaa
    17401 aaggggattt tgccctttct gaaaagctcg tttgcctctt cattggcggc gttcgccgcg
    17461 gcggggtata gccctcctct gttaatggcg tcgcggcaga tatttatgca cttaaaggtt
    17521 tcgtaatcgg gctcaaagaa tgtgagttta cctatttggg caagatttaa gctgcccacg
    17581 ggggactcga atctattggg ataggtaatg gcgtattgaa tggggatgcg catatcgggg
    17641 agccccagct gggctatgac cgagttgtcc gcgaactcaa caagggagtg gacaatgctc
    17701 tccctgtgga cgacgatttc tatgttttcg ggagcaacat caaaaagcca aaccgcttcg
    17761 ataagctcca gacccttatt gaacatggtg gcgctgtcaa cggtaatctt cgcgcccatg
    17821 ctccagttgg gatgctttaa agcgtcctgc aaagtaacgc ttttaagctc atgtcttttc
    17881 tttccgaaaa agggtccgcc ggacgcggtg aggatgattt tctttatttc ctttttatct
    17941 ttgcagccct gaattgactg aaaaatggcg gaatgttcgc tgtcaacggg caaaagctcg
    18001 gcgttgtttt ccttaaccgc ggtttttacg agttttccgc ccgccacaag gctctcttta
    18061 ttggcaaggg cgattctcct gccggctttc gcggcggcaa gggtgggtct gagacccgcc
    18121 atgcccacta tggagtttaa aaccgtgggg ttttctccaa aggcggcgca ctcggtcacg
    18181 ccttcaatac ctgaaagaac cttggtgttt gtgtcagcca attttatttt aagctccgct
    18241 gccgcttttt cgtccgcgag ggcggcaagg cggggtttga atttccttgc ctgttcctca
    18301 agtttttttg catccgaatt ggcagtcaga gcctctacct tcaaactcag cttttcggcg
    18361 acatcaaggg cctgcgctcc tattgagccg gtgctgccga ggataatgag tgagttcatt
    18421 ttgacctccg aatttatacc caaagcatta caaaggcgta aagcacgggc attacgaata
    18481 ttacgctgtc gaacctgtcc atgactccgc cgtgaccggg gagtatgttg ccgtagtctt
    18541 taacgccgaa attcctcttg ataaccgaag cggaaaggtc gcccagcata ctcgtgaggg
    18601 aaagcacagc cgacaagggg gcgacgacaa aatagctcat catagtggac ttgccaggga
    18661 agcagttttt aaatacggcg caaaggataa ggtttaagat aatcgctccg ataacgccgc
    18721 cgacagctcc ctcaatgctc tttttggggc tgatgttggg ggacattttg tgctttccga
    18781 actttacgcc cacgaagtag gcgaaa
//
LOCUS       DAONVQ010000020        18465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2208_length_18465_cov_4.193862, whole genome shotgun sequence.
ACCESSION   DAONVQ010000020 DAONVQ010000000
VERSION     DAONVQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 18465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18465
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2208_length_18465_cov_4.193862"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLD98_02445"
     CDS             complement(<1..673)
                     /locus_tag="PLD98_02445"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HQA05141.1"
                     /translation="MLSKIKSSLTLENIIVRALTSYCVMNIAIIIIYGDDFLSKSYIG
                     RVNFIFACLILVLSFLFLSAFDLLFTDREISKIFRKSHLTIAAMAFFVRVAAETGSFY
                     LFLIFLPFLAALTRYVLPESEKELKILSSVKLKHVKATFGILGLAFFLFTGLYTAFRY
                     WSYNSPCFDFGIWAQMFHNMKTTGLPITTCERYMELSHFAVHFSPIYYLMLPIYFIFP
                     DPATLQ"
     gene            complement(820..1473)
                     /locus_tag="PLD98_02450"
     CDS             complement(820..1473)
                     /locus_tag="PLD98_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05142.1"
                     /translation="MADSAPLYPVLYYSIGFGAVICLAFLQTYTRYVTDKIFFLKASD
                     SSTKGAYRIKGKTLLPKSFLLGVLIVTLKTLWFLVLQIPFLALASYFAYLLFKGLPRN
                     IIYVFVTGCLVLFVIGLFFWFCIVQRYAAARYFFVARKNPSAFGSIKQSTDTMRGRSL
                     KTAVFKLSMLPWIATCLAVIPIPYVLTYCKMSSAVLINEFMCRLKAKPGPAIVLGVK"
     gene            complement(1731..2594)
                     /locus_tag="PLD98_02455"
     CDS             complement(1731..2594)
                     /locus_tag="PLD98_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQA05143.1"
                     /translation="MLKTKHEKGIIISFYAIAAVFMLVASFFDLQINQLLYYPNNPFG
                     VFLDRIGELPPYVAVALALTALFRFPPKLKKWATALLKAIFGIASVAVWIYIFIDFGD
                     KWIIEDENRLIFEIIFGLITGFLSLYLSKYLPEKHMSAFRNLAVFFILIALLTSWPTS
                     VLKDIWGRIRFRDLVAAGSYDGFTPWYIIAGKDGRSFPSGHTTGAAATFMLMGISYFF
                     PSQKENELKYYIAAMVFTFAVGFSRITRGAHYLSDITFAAIIVFTLFLIIRYLYVKRP
                     FGSSKKADTKN"
     gene            complement(2615..4522)
                     /locus_tag="PLD98_02460"
     CDS             complement(2615..4522)
                     /locus_tag="PLD98_02460"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HQA05144.1"
                     /translation="MYFKPTKEMIEKAALIKSVTANINAKKTHKFKIDVPLDFNKGIA
                     DGYEMSSPDGQIKARLYFKNGLRYEVIFGGRTVIEPSEMGIEIKNHKLGQNVFVGQPE
                     IHEHDAEYTVRGKHNKTRDLHRAYRFPVHHFPTGLKYIVEFRLWNDGAGFRFEFPKGS
                     RMLIFWENTEINVVRDSVCLYQTDVKKLQSKTLRAEIKDLPEDLRIAYLTTFELSGVG
                     YMILTESNLINYPGAALMHESSGKFKIDFWDSGKFFVKDCATPWRIAILAHDLNTLVN
                     NEVVKHAADPPSPELENADWIKPGKAAWSYFHDKATSRSYETILEFNKQLDDLGYDFS
                     LIDSGWRKWSLTESTAIRKVKNVIKQKPESVDIWVWKSIEMGPFIPLYRQWLLKKLQS
                     IGVVGVKLDHIETETQWTINVYRRFLEDAAKHKLMVIYHNPNKPTGLTRTYPNMLSRE
                     AVRGLQCAADADDNTIIPFTRFAIDGADYTPFCPSLPDRCTNTTVSHALANLIVFTSP
                     FMTIAEHPKNLKGTVYQDFFKTIPVCWDETIVLPQSELGRLAAFARRHEDLWYIGVQA
                     GNIEDFEFSFTPHFLDDGKEYKLEMFTDSEEKDSKDTVRCERIVTKDDTIKVNIKTAG
                     GFAGRFIPINQ"
     gene            complement(4574..6049)
                     /locus_tag="PLD98_02465"
     CDS             complement(4574..6049)
                     /locus_tag="PLD98_02465"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQA05145.1"
                     /translation="MSKFSPRRFKGAAIFLAKALLMIGSVFLFLMTLIYFYREAMFPA
                     RGNYVLAFLYACILMTLSTTYECFKIGISRIKELVLSFAIAAGLTNFFTYLILSLIES
                     KLLSPVALIGLTLAQIIYGIVIYTCANKIYFVIFPISSCVFVSSGSEYEQEILNKLQT
                     NRSRVHIHKVINQDAGIEAIQNEMNKYNTIMIGDIDAELKNFMIKYCFEKNKSMYILP
                     SVQDIVVNASTEYQFGDTVMFYSKSRSMSIEQLAIKRLMDLAISIVGLILASPIMLII
                     ALLIKLYDGGPVFFKQERYTRNEKVFTLIKFRSMIVDAEKDGAAFTVNNDSRITPIGR
                     FIRATRLDELPQLINILKGDMSFVGPRPERIENVENYSQKMPDFKYRMKVKAGLTGYA
                     QIFGKYNTSCEDKARMDMLYIQNYSLFLDLKIIFYTFKVIFMKESTEGFATSGLENKK
                     TEAEKLPAAEDKAAEKDKRIQLDVNDYIPENLDRKNFAGNN"
     gene            6606..7322
                     /locus_tag="PLD98_02470"
     CDS             6606..7322
                     /locus_tag="PLD98_02470"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HQA05146.1"
                     /translation="MIESVIALIAFLILSPVVRAMADRQITVRSKKIATFGYSEEVRL
                     QREAEFASAMEKIKTPKALILIMLVLAIPGALIFLTNNPIEVKIEYSLYLLLIINIAV
                     VDQLVKRIPNELLLSLLIVRTASIIYAIINGGSIKNLIVSSIVGFALALIIFDIPSVF
                     HVYIGAGDVKFCAVIGYCFGFLGFIEASFAMGVAMLIVLIYLKATKKGSIKSKIALGP
                     YLALGAVVHMLTSFTSLLTR"
     gene            7403..7588
                     /locus_tag="PLD98_02475"
     CDS             7403..7588
                     /locus_tag="PLD98_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HQA05147.1"
                     /translation="MLMNWLRDEEGQAMVEYGLIIALIAIAVIGALAILGPKIAKLFD
                     DAASEVASATSYPAGAD"
     gene            7768..8181
                     /locus_tag="PLD98_02480"
     CDS             7768..8181
                     /locus_tag="PLD98_02480"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HQA05148.1"
                     /translation="MRKSFLKREKGQSIVEFALVLPLILLIVCGIIDFGWLFYNQLTL
                     NNACRDAARYAVVNATKSDLVSAVDEKAEQESRGLFKKGINTQVTFTNPTNPLEGDVV
                     VTLTAEMNALTPVLFIISGGPTRQLVSTVVMKAES"
     gene            8236..9024
                     /gene="cpaB"
                     /locus_tag="PLD98_02485"
     CDS             8236..9024
                     /gene="cpaB"
                     /locus_tag="PLD98_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HQA05149.1"
                     /translation="MKKVYLIATVIALITGLATFFIFRSVSGKKTTDEMVDVVVAAVE
                     IKENTPITADMLEVVKKPLAEAKPGFLSRIEDVEGSYAMYTIPKGEQVVRASILKPSP
                     IGGPDQNKGKLSLELSPGEYALSVPVTLIDAVSYYPREYDYVDVYLVEKVHPNDIGQT
                     DVNGSVYEADDMKTSLLLEGVRILRVSNYQQEDLSAANGTKITSYTEVTLRLNKEQVL
                     KLASAMNQGTIKLALTAFGENEGETTTAPPTTAPPATEETSEAA"
     gene            9075..10271
                     /locus_tag="PLD98_02490"
     CDS             9075..10271
                     /locus_tag="PLD98_02490"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA05150.1"
                     /translation="MEKLNIVVLSTDLDLRIQVKNSINSERMVIAGFADFNDAGLIKL
                     SGLYPDVIIVAVKGPVEDHVFNFVQHVLSTVQNCFALLMNDRIDVDLVNKAAQYGFRM
                     VLTLDTQPAELEDIISKIYSLELERNMDKNASKRVRSKVISVFSGKGGTGKTTVAINL
                     AAALSKAGKRVFLIDADLQFGDVALAMDIEPKDTIVELVQDRNGVTIENINSFSIVHN
                     TGVTVLCGPDSPEFAEYITGEDIERIIDIARPYFEYIIVDLPPSFNDYSIAALENSND
                     ILLVYNNDILSLRNAKACITILEKLHLKDKVEIVINKNLQGLISVRDFEKMFNMSVIA
                     SVPHDVKAATASINKGLPIFVAHSKSLISKEIGNLANKIIQKHTGILPIDMIQPKKRF
                     SLFKKA"
     gene            10348..11682
                     /locus_tag="PLD98_02495"
     CDS             10348..11682
                     /locus_tag="PLD98_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HQA05151.1"
                     /translation="MSLLEKLDRSGNQARETNNSKETVSALPPDPYAAVKQKIHRDLI
                     NSMNKQNLGTVDKDTMMRLIEEHVNDEVNGIPRIDRPKIVVEIYNDVMGYGPIEELLE
                     DQRISEIMINGPDNIYIESRGKLQLTGLKFRDEQHLMSVIDRIVSNVGRHVDEATPMA
                     DARLPDGSRVNVIIPPLSLIGPVVTIRKFGKNPITAQQLIKFGSLNHNMLAFLEACVR
                     GKLNIVVSGGTGSGKTTLLNVLSSFIPSDERIITIEDAAELQLKQDHVITLEARPANI
                     EGKGAITIRSLVKNALRMRPDRIIVGEVRAEETLDMLQAMNTGHDGSLTTIHANSPRD
                     SIARIETMVMMSGMDLPMKAIRNQIASAIDIIIQQSRLRDGTRKVTSISEIVGMEGDI
                     VSMQDIFVYETDGKLDADGKFIGRFRATGARPHCIEKIKSNGVNVNSDWFTD"
     gene            11663..12646
                     /locus_tag="PLD98_02500"
     CDS             11663..12646
                     /locus_tag="PLD98_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQA05152.1"
                     /translation="MTGLQIRSLIFAVLMGFTVFVGVVIFYNYFTKDKRAVLRRLLKI
                     RGRQNTIFDAIELEKERKSESIEERLGKRGATGQRKFVDRILNELVLAGIAMRPEEFA
                     LIWILLVFVPSGIAALFGQKIMPAATLAFLGAIAPVIYLSQKKKKRVAEFDSQLGDAL
                     MVITNCLRSGLSFQQSLETIVNEMSPPISSEFSRVLIEVKYGNPLEVALNNMVERLKS
                     ADLLIAISAVNIQRQTGGNLSEILETLANTIKERMRIKKEIKAITAQGRMSGLVVGLL
                     PVGLTGILMVVAPDYITTLFTDPRGQIMIGIAAVMEMIGALFIKKIINIKY"
     gene            12679..13617
                     /locus_tag="PLD98_02505"
     CDS             12679..13617
                     /locus_tag="PLD98_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQA05153.1"
                     /translation="MEILVILYSILAFFVSLSILGVFGQQQDIKQRRIEYIDGINKMP
                     VFEELDKSFFERFIAPRLKNLRNKMPKISVGKNQGDASSAKYEQVERLLRLAGVNMSA
                     PDFMFLKNIGAIILTIIALIVSLIIKTTMTNRLLITAMGLLIGMIIPTYILRSKVTSY
                     QEAIRNQLPDALDLLGVCIEAGLSFDAALIKVAEKLEGPFINELKILYREIQMGKPRR
                     EALRSLGDNTNIPELKTFAAALAQADQLGIPINNVMKVQSEQLRETRRQMAREKGMKA
                     PIKMMFPMVGFIFPVIFIIILGPSVLTIMDSFLISR"
     gene            13623..13970
                     /locus_tag="PLD98_02510"
     CDS             13623..13970
                     /locus_tag="PLD98_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HQA05154.1"
                     /translation="MKTLRAYAADGRTLVRNVELANTFFTRLKGLMFRESLPNDTGLL
                     IYPCCQIHTYFMKFSIDVVYLNRDFKVVFIDRNIQPGVSVRRIREAKCVLELNAGEAD
                     RLGIAVDDVLTFA"
     gene            14028..15338
                     /locus_tag="PLD98_02515"
     CDS             14028..15338
                     /locus_tag="PLD98_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05155.1"
                     /translation="MYQNPDDRLSVDANKDGNQKETVSVNKADGQNSVLNLDDGLSCP
                     IRTSVTFKKALFGGLDPDEVYAYIDEQFENYNNTCQMYAGRLEELKNNITLISRERDW
                     LKIQNQDAVKRAEAAEERLMASQKAFDEELALAQQAEEKARREAEEASRAAAEAKAMA
                     LEAERNSKIMSEQLSKAKDAQRRAEEELNKAHTKLRLAEERAVLAEEKMKEALKELAS
                     QNDSNELQEKYNELSSKYNQLLEDSRRLKEFEAGLAQSAIEKENLQKKIESLNAQIEK
                     LKEENQRLSGVISESNLQLEAFEKLKVDYSAVNARLSVAEAELSSVSVELSKKTDENE
                     KLQSTLADLRIRIASLEQQNDNLEKEISALRENLRVQMYEFAQKKNEYETAAANERLN
                     LIKLIQVHSYHLKQSEMLLNELRTQFSHAIETFKDLEKEDAGQE"
     gene            15368..17107
                     /locus_tag="PLD98_02520"
     CDS             15368..17107
                     /locus_tag="PLD98_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HQA05156.1"
                     /translation="MKKRLRSFWKEESGVAVIFVAISMVVLMSFTALAVDYGMVYYHR
                     SELQTALDSAALAAAQKLPDKEAARRTAIEYVRKNGIEVKSDDEIIVDFLQNDTIVRV
                     SSSVETKTSFARIFNVDTIKSVAVAAAQTKEVKQGQQSFDHLLFSGSRDDRLFLDSQF
                     EIFGSVHTNNKFFFSPSNGVIHGEVSAVVPGSSYNGGFRAGSINPNASYIPMPDFSDS
                     IAKYVPPFPTETAESNFGYAANKIRGIRGYVSPAYVNSLNQQNITLEGKTEYSGPLNI
                     KAWGSTTIIGEIYVKGSASFQGKLIMNSPNDLLCVDGDLNIGGGLFGKGTLIVDGDLT
                     ISGGGPYTFDGDIYVAGNFRTNAGLNATGKIVCKGNMNISQSFNFYGPSSNIPGVIYC
                     DGNLSANLYKGKADIICQGTISFTGNSAEHDGLLYSAKQINVYTRLISNNITILSKDD
                     ITVNSNCTIAKGVIYAGKNFNCYTALEGSVYVFAENNLNYSGYANNNTQLDIAFYAIN
                     GNISISLSKTNVTGIIYAPKGNVHMGGSIEIYGSIIANSFTGSFSKLTIRRNENGNPL
                     DVGKTVKRTVLVQ"
     gene            17394..>18465
                     /gene="alaS"
                     /locus_tag="PLD98_02525"
     CDS             17394..>18465
                     /gene="alaS"
                     /locus_tag="PLD98_02525"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQA05157.1"
                     /translation="MQWTGLNEIREKYLSFFESKGHLRLPSFSLVPEGDKSLLLINAG
                     MAPMKKYFTGEVTPPSKRVTTCQKCIRTPDIERVGKTARHGTFFEMLGNFSFGDYFKR
                     EAISWAWEFCTKVLEIPVDRLYATVYLEDDEAYDIWTKEIGIDETHIKRMGKEDNFWE
                     LDSGPCGPCSEIYFDMGEEFGCGSPDCGVGCDCDRYVEFWNLVFTQFEKDSEGNYTPL
                     AHPNIDTGMGLERIACIIQGAPNLFEVDTVKGIMEHISSIAGVKYHEDPKMDVSLRVI
                     TDHIRSTVMMVGDGIMPSNEGRGYVLRRLLRRAARHGRLLGIDRPFLSELAESVIKFN
                     AGAYPDLTEKHDYIINVITHEEE"
BASE COUNT         5479 a         3912 c         4096 g         4978 t
ORIGIN      
        1 actgaagcgt cgcggggtcg gggaagataa agtatatggg cagcatcaga tagtatatgg
       61 gcgagaaatg caccgcaaaa tgcgaaagct ccatatatct ttcacatgtg gttatcggca
      121 gacccgttgt tttcatgtta tggaacatct gtgcccagat accgaaatca aaacagggtg
      181 aattgtagct ccagtatctg aacgccgtat aaagtccggt gaaaaggaaa aatgcaagac
      241 ctaaaatacc gaaggttgct ttaacatgtt tcaattttac agaagacaga attttaagct
      301 ctttttcact ttcgggcaga acatatcttg ttagagcggc aaggaaaggc aggaaaatca
      361 aaaataaata aaaactgcca gtttctgcgg caacacgcac aaaaaacgcc attgcggcta
      421 tagtcaggtg gctttttctg aaaatcttgc tgatttctct gtcggtaaaa agaaggtcaa
      481 aagctgaaag aaaaagaaaa gacaatacga gaattaagca agcaaaaata aaattaaccc
      541 tgccaatata gctcttgctg agaaaatcat ctccgtaaat aatgatgatc gcaatattca
      601 ttacgcagta gcttgtcagg gctcgtacga ttatattttc aagtgtcagc gaggacttaa
      661 ttttgcttaa catgccgcct ccgaaaattt atgttaaatc ctgttaacta atttatccca
      721 aaaatattat cataataaag acaaatatgc aaggcatgct gatgcataag aaaacccgta
      781 aatccacgga tttatgggta aataagaatt tgtataacct tatttaactc ccagtacaat
      841 tgcgggaccc ggttttgctt ttagtctgca cataaactca ttgataagca ccgcagacga
      901 cattttgcag taggtcagca catagggtat cgggataaca gcaagacatg tcgcaatcca
      961 cggcagcatg ctgagtttaa aaactgccgt tttaaggctc ctgccacgca ttgtatctgt
     1021 gctttgcttt attgagccaa aggcgctggg atttttgcgg gccacaaaaa aatacctcgc
     1081 tgccgcgtaa cgctgaacaa tgcaaaacca aaagaatagc ccgattacaa agagcactaa
     1141 gcagcccgta acaaaaacat aaataatatt gcgcggcagt cccttaaaaa gcagatacgc
     1201 aaaataagaa gcgagggcta aaaagggtat ctgcaaaacc agaaaccaaa gcgttttaag
     1261 cgttacaatc aaaactccaa gcagaaaaga tttgggcaga agtgtttttc cctttattct
     1321 gtatgcgcct ttggtgcttg agtcagacgc ctttaaaaag aagattttgt cggttacata
     1381 tcttgtgtat gtctgcaaaa acgcaaggca aataacggct ccaaagccta ttgagtaata
     1441 caaaacagga tagaggggag cggagtccgc catattaaat aagctgctga caaaaggatg
     1501 aaatgcggaa agcgccagca gacaaacggc gccaaggcat aatagtgata atagaaaggc
     1561 tgtcataata agtacaaagt aactgaaaac cctgcccgtt attagcccct tagccctaag
     1621 cttatattta gaaaacaagc tcagcacacc cccttttaag ttctgttagc gcctacttaa
     1681 aagtatggga tggctgagca gtttttatgc tttaaatgcc tctaagttac ttaatttttg
     1741 gtatctgcct ttttagagct tccgaaaggt cttttgacat ataaatagcg gataatcagg
     1801 aataaagtaa agacaatgat tgcggcaaat gtaatgtccg aaagataatg cgcaccacgg
     1861 gtgattcttg agaagcctac tgcaaatgta aataccatgg cggctatata gtatttaagc
     1921 tcattttcct tctgtgacgg gaagaagtat gaaataccca taagcataaa tgttgctgcg
     1981 gctccagtgg tatgacccga cgggaaggag cggccgtcct tgcctgcgat tatgtaccag
     2041 ggggtaaagc cgtcgtagct gcctgccgcg acaaggtctc tgaatctgat tctgccccag
     2101 atgtccttta agacgcttgt gggccaggag gtgaggagag cgatgagaat aaagaacact
     2161 gccaggtttc tgaacgcgct catgtgcttt tcgggcagat attttgaaag gtaaaggctt
     2221 aagaatcctg tgataagtcc gaagataatc tcaaaaatca gcctgttttc atcctcaatg
     2281 atccacttgt cgccgaagtc aataaatatg taaatccaaa cggcgactga ggcaatgccg
     2341 aatatagcct tcaatagtgc ggttgcccac ttctttagtt tcggcgggaa tctaaagaga
     2401 gccgtaagtg ccaaagcgac agctacatac ggcggcagct cgccgattct gtcaagaaaa
     2461 acgccaaaag gattgttggg ataataaagc agctgattta tctgtaaatc aaagaatgac
     2521 gccaccagca taaaaacagc ggcgattgca taaaagctga ttattatgcc cttttcatgt
     2581 tttgttttca gcatagaatt ccctctttcc aaagttattg attaatcggt ataaatctgc
     2641 cggcaaagcc gcccgcggtt ttaatattga cctttattgt atcatctttt gtgacaatcc
     2701 gttcgcatct gactgtatcc tttgagtctt tttcctcact gtcagtaaac atctcaagtt
     2761 tatactcctt gccatcatcc aaaaagtgcg gagtgaaaga aaactcaaag tcctcaatgt
     2821 tacccgcctg cactccaatg taccataaat cctcatgcct tctcgcaaaa gcggcaaggc
     2881 ggccgagttc gctttgaggc agaacaatgg tttcgtccca gcagacgggg attgtcttaa
     2941 agaaatcctg ataaacggtg ccctttaaat tctttgggtg ctcggcaatc gtcataaagg
     3001 gcgatgtaaa aacaatgaga tttgccaatg cgtggctgac cgttgtgttt gtgcacctgt
     3061 cgggcagaga cggacagaag ggcgtataat ccgctccgtc aatcgcaaat cttgtaaagg
     3121 gtatgattgt gttgtcatcc gcatccgccg cgcactgcag acccctgaca gcctcccttg
     3181 acagcatgtt gggataagtc cttgtaagac ccgtaggctt attggggttg tggtatatta
     3241 ccatcagttt atgttttgcc gcgtcctcca aaaatcggcg gtaaacattt atcgtccact
     3301 gagtttcggt ttcaatgtgg tccaacttta cgcccacaac gccgattgac tgaagtttct
     3361 ttaaaagcca ctgcctgtaa agaggaataa aaggacccat ttcaatggat ttccaaaccc
     3421 aaatatcaac gctttcgggc ttttgcttta taacattttt aacttttctt atagctgtgc
     3481 tttcggttag agaccatttc ctccagccgg aatctataag cgaaaaatca tatcccaaat
     3541 catcaagttg cttattaaac tcgagtattg tttcgtagct cctgcttgtc gccttgtcgt
     3601 ggaagtatga ccatgcggct ttgcccggtt taatccagtc ggcattttcc agttcgggcg
     3661 acggtgggtc tgccgcatgc tttacaacct cgttgttaac aagtgtgtta aggtcgtggg
     3721 cgagaatagc gattctccag ggagttgcgc agtcttttac aaagaacttg ccagagtccc
     3781 aaaaatcaat tttgaatttg cctgaacttt catgcattaa agccgcgccg gggtagttta
     3841 taaggtttga ttctgtcaga atcatatacc cgacgccgct tagctcaaat gtggtaagat
     3901 acgcaatcct taaatcctct ggtaaatcct taatttcagc cctgagtgtc ttgctttgca
     3961 gctttttaac atcggtctga tataagcaaa cagaatccct taccacattt atttcggtgt
     4021 tttcccagaa aatcagcatc cttgagccct taggaaactc gaatctgaat ccagcgccgt
     4081 cgttccatag cctaaactca actatgtatt taagccctgt ggggaaatga tgaacgggga
     4141 atctgtatgc tctatgtaaa tcccttgtct tattatgctt gcccctgacc gtgtattcgg
     4201 cgtcgtgctc atggatttcg ggctgaccta caaagacatt ctgacccagc ttatgattct
     4261 ttatctcaat acccatttcg gatggctcaa taacagtcct gccgccaaaa atgacctcat
     4321 accgtagacc gtttttgaaa tacagccttg ccttgatttg accgtcgggg gaggacattt
     4381 catatccgtc ggcaatgccc ttgttaaagt caagcggaac atctatttta aatttatgag
     4441 tttttttggc gtttatattt gctgtgacgg attttatcaa tgccgccttt tctatcattt
     4501 ctttagtggg tttaaagtac atcttgagcc tccgatttta caaaaaaaga ggtgagtgaa
     4561 tcacctctgt attttagttg tttcccgcaa aattctttct gtcaagattt tcgggaatat
     4621 aatcatttac atcaagctga attcttttgt ccttttcggc agccttatct tccgccgcgg
     4681 ggagcttctc agcctcggtc tttttattct caagaccact ggtcgcaaag ccctcggtgc
     4741 tttccttcat aaagataacc ttaaaagtat agaagattat cttgaggtca aggaacagcg
     4801 agtaattctg aatatagagc atgtccattc gggccttgtc ttcgcagctg gtgttgtatt
     4861 tgccgaatat ctgggcatag cccgtaagac ctgccttgac cttcatgcgg tatttaaagt
     4921 cgggcatctt ctgggagtag ttttcaacat tctctattct ttcgggtctg ggacctacaa
     4981 aggacatatc gcccttgagt atatttataa gctgcggcag ctcgtccagt cttgtggctc
     5041 tgataaatct gcctatgggc gtaattctcg agtcgttgtt tacggtaaat gccgcgccgt
     5101 ccttttcggc gtcgactatc atgcttctga acttaataag ggtgaatacc ttttcgtttc
     5161 ttgtatatct ttcctgctta aagaatacgg gaccgccgtc gtagagcttg ataagaagcg
     5221 caataataag cataatgggg gaggcgagta tcagaccaac aatagaaatc gctaggtcca
     5281 taagcctctt tattgcgagc tgttctatgg acattgagcg gctctttgag tagaacataa
     5341 ccgtgtcgcc gaactgatac tctgtcgatg cgtttaccac aatatcctgc accgaaggca
     5401 gaatatacat tgacttgttc ttttcaaagc agtatttaat catgaaattc ttgagttccg
     5461 catcaatgtc gcctatcatt attgtgttgt atttgttcat ttcattttga atagcctcaa
     5521 tacccgcgtc ctgattaatg accttgtgaa tatgaacacg gcttctgttg gtctggagct
     5581 tattcaaaat ttcctgctcg tattccgagc ctgagcttac aaaaacgcag ctgcttatgg
     5641 ggaagattac aaaataaatc ttgtttgcgc aggtgtaaat cacaattccg tagataatct
     5701 gcgcgagagt aagaccgatc aatgcaacgg gggagaggag ctttgactca atcagtgaaa
     5761 gtataaggta tgtgaagaaa ttggtaagtc ccgctgcgat agcgaatgag agaacaagct
     5821 cctttattct gcttatgcct atcttaaagc attcataggt tgtggaaagg gtcatcagaa
     5881 tgcaggcata cagaaaagcc agcacatagt tgcctcttgc ggggaacatc gcttcacgat
     5941 agaaataaat tagcgtcatc aaaaaaagga acacagatcc aatcatcagc agcgcttttg
     6001 cgaggaatat ggctgcgcct ttaaatctgc gtggagaaaa cttagacata ttaaaactcc
     6061 tattacatta aaataataag atgtcaggcg tcaaaagaaa aaatttcctt aaaatttatt
     6121 taagatatta tacaattaac tgacgaataa agtcaataag agcatatagg gcaaaattct
     6181 tgcgatgagg aattaaataa gcttaatcgg aatatattga cagtacaagc agttgaacca
     6241 aactttttta tcaattcggc tttatattta tacggcagaa caataataaa attcccgctt
     6301 tataaatatc attataacca ggtgcttgaa agagcttgtc taaaaaaatt gcgtcaaaat
     6361 ccgctttttt gtgacccgat taccgcaatt tagtgctctt tggcggtctt ttgtaaataa
     6421 aggagattta tccctaaaat tttatgcact ttttggtcaa tttttaagaa aaatcataaa
     6481 ttttctattg acacttgtcc aatcgtgtga taatttaata acagcccaag gaaatttatg
     6541 aaaatttgta tatttcacaa ggaatttacg cagttgagta tattttacag ccggaggcac
     6601 ttaatttgat tgaatctgtt atagctttaa ttgccttctt aattctttcc cccgtggtaa
     6661 gagccatggc tgacaggcaa ataaccgttc gctcaaaaaa gatagccacc ttcggctaca
     6721 gcgaagaggt aagacttcaa agggaagctg aattcgcctc cgccatggag aaaatcaaaa
     6781 cccccaaggc attgattctt ataatgcttg tgcttgcgat tcccggggcg ttaatcttcc
     6841 taacaaataa ccctatcgaa gtcaaaatag agtattccct ttacctatta ttaatcatca
     6901 acattgctgt tgttgaccag cttgttaaaa ggatacccaa tgagctgctt ttatccctac
     6961 ttattgtccg cactgcttcc ataatttatg caataataaa cggcggcagc atcaaaaacc
     7021 ttattgtcag ctctatagtc ggttttgcat tggctcttat tatttttgat atcccttctg
     7081 ttttccatgt ttatattgga gccggcgatg ttaagttctg tgcggtaatc ggctactgct
     7141 tcggattttt gggctttatc gaggcctctt tcgcgatggg cgtagcgatg ttaattgttt
     7201 tgatttatct caaagccaca aaaaaaggct caatcaaatc aaagattgct ttaggtcctt
     7261 atctggcact cggcgcggtg gtgcacatgc ttacatcctt tacttcactg cttaccagat
     7321 aatacatcaa ttactttgga tttaattccg cataaagcgg gttaaattat aaacataata
     7381 aaaataaaaa ggaggaaaga ttatgttaat gaattggctt cgcgacgaag aaggccaggc
     7441 aatggtagag tacggcctta tcatcgccct catcgctatc gctgttattg gcgctctcgc
     7501 tatccttgga ccgaaaattg ctaagctctt cgatgatgct gctagcgaag ttgcaagtgc
     7561 cacatcctat cccgccggcg ccgattaatg gctaataatt attgctcaaa ggcaaattaa
     7621 atcagcacat tatgggtctt agacccgtcc tgttagcggt ggaatgttat tccctgcggg
     7681 atgaaattaa agacgcagga ttttcctgcg tctttgtttc attatcctta cgcaaaactt
     7741 aatattgttt gtaaagcggt ggtgaatatg agaaagagtt ttcttaaacg agaaaaaggg
     7801 cagtcgattg ttgaattcgc tcttgtgctg ccgcttattt tgcttattgt gtgcggaatc
     7861 atagatttcg gctggctttt ttataatcag cttaccttaa acaatgcctg ccgcgacgca
     7921 gcaaggtacg ctgtagtcaa tgccacaaag tccgatttgg tatccgctgt ggacgaaaaa
     7981 gcggagcagg aatcacgagg gcttttcaaa aaaggtatta atacacaggt tacctttaca
     8041 aaccccacta accctcttga aggtgatgtg gttgtgaccc tgaccgcgga aatgaatgcc
     8101 ttaacgcccg ttctttttat aatatcgggc ggacccacaa gacagcttgt ttctacggtc
     8161 gttatgaaag cggaaagcta agcaaatgct ttaacatata aattcaaaag ctcttgaagg
     8221 gatgtaaagt aatctatgaa aaaagtttat cttatagcaa ccgttatagc gcttattaca
     8281 ggccttgcga catttttcat cttcaggtca gtcagcggca agaagactac cgatgaaatg
     8341 gtagatgttg ttgtagccgc tgtggaaatc aaagaaaaca cacccataac cgcagatatg
     8401 ctggaagttg ttaaaaaacc cttggcagag gctaaacccg gctttctgtc aagaatagag
     8461 gatgtggaag gcagctatgc aatgtacaca attcctaagg gtgaacaggt tgtcagagcc
     8521 tcaatactta aaccaagccc cataggagga cccgaccaga ataaaggtaa actttcgctc
     8581 gaattgtctc cgggtgaata tgcattatcc gttccggtta ccttaattga tgcggtaagc
     8641 tattatccca gagaatatga ctatgtggat gtctatcttg tagaaaaggt gcatcccaac
     8701 gatataggtc agacggatgt aaacggcagc gtttatgagg cggatgatat gaaaacctca
     8761 ctgcttcttg aaggcgtccg catactgaga gtttcaaact atcagcagga agacttatct
     8821 gccgcaaacg gcactaagat aacctcttat actgaggtca cattaagact caataaagag
     8881 caggtgctta agctcgcatc tgccatgaat cagggcacaa tcaagcttgc tcttaccgcc
     8941 ttcggcgaaa atgagggtga aacaacgacc gctccgccaa ccacagctcc tcccgcaact
     9001 gaagagacct ccgaggccgc ttaaagcaag ttttaggttt aatataaaaa acctgctaat
     9061 cgaaaggaac cgatatggag aagctaaata tagttgtact ttcaactgat ttggatttaa
     9121 gaattcaggt caaaaacagc ataaactccg agcgcatggt cattgccggc tttgcggatt
     9181 ttaacgacgc cggacttatt aagctctccg gtctttaccc cgatgttatc atcgttgctg
     9241 taaagggccc ggttgaggac catgttttca actttgttca gcatgttctt tcaaccgtac
     9301 agaactgctt tgcccttttg atgaacgacc ggattgatgt cgatttggtc aacaaagcgg
     9361 ctcagtacgg ctttagaatg gttctgaccc ttgatactca gcccgcagag cttgaggata
     9421 taatcagcaa gatttactcc cttgagcttg agcgcaatat ggataagaac gccagcaaaa
     9481 gggtccgctc taaggttata agtgttttca gcggcaaggg cggaacgggc aaaaccacgg
     9541 ttgccataaa ccttgcggcg gctctttcca aggctggcaa gagggtattc ctgattgacg
     9601 cagacctgca gtttggcgat gtagccctcg ccatggatat tgagcccaaa gacactatcg
     9661 ttgagctggt tcaggaccgc aacggtgtca caattgagaa tatcaacagt ttttcgattg
     9721 tgcacaatac cggcgtaacc gtgctttgcg gaccagacag ccccgagttc gcggaataca
     9781 tcacaggtga ggatattgag aggattatag atatagcaag gccgtacttt gagtatatca
     9841 ttgtcgacct gcccccctca ttcaacgact attccatcgc cgcgcttgaa aacagcaacg
     9901 acattctgct ggtttacaac aacgacattc tctcactcag aaacgcgaag gcatgcatta
     9961 ctattcttga aaagctgcat ctcaaggata aggtagagat agttataaac aagaatctgc
    10021 agggtcttat aagcgtcagg gactttgaaa agatgttcaa tatgtccgtt attgcttccg
    10081 taccccacga tgtaaaagca gcgacggcaa gcataaacaa gggtctgccg attttcgttg
    10141 ctcacagcaa gtctcttatt tcaaaagaaa tcggtaacct tgctaacaaa ataattcaaa
    10201 agcacaccgg cattttgcca attgatatga ttcagcccaa aaagcgtttt tcccttttta
    10261 agaaagcctg aaaagggaat gctaatgtaa tttaactttg agttatctcg aaattaaatt
    10321 gcatggcaat gtaaggaagg attcactatg agcttacttg aaaaactgga cagaagcggc
    10381 aatcaagcca gagaaactaa taacagcaaa gaaaccgtat ccgctttgcc ccccgacccc
    10441 tatgctgcgg ttaagcagaa aattcaccga gatttaataa actcaatgaa taaacagaac
    10501 ctcggcaccg ttgacaaaga tacaatgatg cgcctcattg aggagcatgt aaacgatgaa
    10561 gtcaacggca ttccgagaat cgacagaccc aagattgttg ttgaaatcta caacgatgtt
    10621 atgggctacg gtcccattga ggagcttttg gaggaccaga gaatctcgga aatcatgatt
    10681 aacggtcccg acaatattta cattgaaagc cgaggcaaat tacagctcac gggacttaaa
    10741 ttcagagatg aacagcatct tatgagcgtt attgacagga tagtatccaa tgtcggcaga
    10801 catgtcgacg aggcaacgcc tatggcagac gcccgtttgc ccgacggctc ccgtgttaat
    10861 gtcatcattc ctccgctgtc actcattggt cccgtagtta ccataagaaa gttcggtaaa
    10921 aacccgatta cagctcagca gcttattaaa ttcggttcat taaatcacaa tatgctggct
    10981 ttccttgagg cctgcgtaag aggaaagctg aacatagtcg tatccggcgg taccggcagc
    11041 ggtaaaacga cgctgctcaa tgtcttgtcc tcatttatcc cgtcggatga gcgtattatc
    11101 accatagagg acgcggcaga gctacagctc aagcaggacc atgttataac tcttgaggcg
    11161 agacccgcca acatcgaagg caagggcgcc attacaatca gaagccttgt taagaacgcg
    11221 ctgcgtatga gacctgaccg aatcatcgtc ggtgaggttc gcgcggaaga aactcttgac
    11281 atgcttcagg ctatgaatac gggtcacgac ggctctctca ccaccatcca cgccaactcc
    11341 ccgagagact caatagcccg tatcgaaaca atggttatga tgtcaggtat ggatctgccc
    11401 atgaaggcaa tccgcaacca gatcgcgtcc gctatcgaca ttatcattca gcagagccgt
    11461 ctcagagacg gtacaagaaa agtcacctcc ataagcgaaa ttgtcggtat ggaaggcgac
    11521 atcgtaagca tgcaggacat ctttgtatat gaaacggatg gtaagcttga cgccgacggc
    11581 aagttcatcg gcagattcag agcaacaggc gcaagacctc actgtataga aaaaattaag
    11641 tcgaacggag tgaatgtaaa cagtgactgg tttacagatt aggagcctta tattcgctgt
    11701 tctgatgggc ttcaccgtct ttgtgggagt agttatcttc tataattact ttacaaaaga
    11761 taaaagggct gtattaaggc gacttttgaa aatcagaggc aggcagaaca cgatatttga
    11821 cgccatagag cttgaaaagg aaagaaagtc tgagtcgata gaggagcgcc tcggcaaaag
    11881 gggagccacg ggacagcgca agtttgttga caggatactc aatgagcttg ttcttgcggg
    11941 catcgccatg agacctgagg agtttgcgct tatatggatt ctccttgttt tcgttccttc
    12001 cggtattgcc gcgcttttcg gacagaaaat aatgcccgcc gcaacacttg cgtttctcgg
    12061 agccattgct cccgtaatct atttgtcaca aaagaagaaa aagagagttg cggaatttga
    12121 cagccagctg ggcgacgctc ttatggttat caccaactgt ttgcgctccg gtcttagctt
    12181 ccagcagtct cttgaaacca tcgtaaacga aatgagtccg ccgataagct ccgaattctc
    12241 ccgcgttttg attgaggtca aatacggcaa cccgcttgaa gtcgccctta acaatatggt
    12301 tgaaaggctc aagagcgccg acctgcttat tgctatttca gctgttaaca tccaaaggca
    12361 gacaggcggt aacctttccg aaattcttga aaccttggcc aacacaatca aagagagaat
    12421 gagaatcaag aaggaaatca aggcaattac cgcccagggc agaatgtcgg gtcttgtagt
    12481 cggtttgctt cccgtcggtc ttacgggaat acttatggtt gttgctcccg attatataac
    12541 tacattgttt acagacccca gaggtcagat aatgattggc atcgccgctg ttatggaaat
    12601 gatcggcgct ttgtttatta agaagataat caacattaaa tactaaggct ttttgccctt
    12661 acgaaaggca ggtctattat ggaaatactg gtaatattgt attcgattct cgcattcttt
    12721 gtttccctgt ctatcctcgg cgttttcggt cagcagcagg atataaagca gagaagaatt
    12781 gaatatatag acggaataaa taagatgcct gtttttgagg aattggataa atccttcttt
    12841 gagcgcttta ttgctccgcg cctgaagaat ctgcgcaaca agatgccgaa gatttcagta
    12901 ggcaaaaacc aaggagatgc ttcgagcgca aaatacgaac aggtcgaaag gcttttgcgt
    12961 cttgcgggcg ttaatatgtc cgctccggac tttatgtttt taaagaatat cggagcaatt
    13021 atccttacca taattgcgct gattgtatca ctgattatta aaaccacaat gactaacagg
    13081 ctccttatta ccgcaatggg cttgctgatt ggcatgatta ttcccacata tatactgcgc
    13141 tcaaaggtta caagctatca ggaggcaatc aggaatcagc tccccgacgc gcttgacttg
    13201 ctcggcgttt gtattgaggc gggtctcagc tttgacgccg cgctcataaa agttgcagaa
    13261 aagctggaag gtccgtttat aaacgaattg aagatactat acagagaaat ccaaatggga
    13321 aagccgagga gagaggcatt aaggtccttg ggagacaaca caaatattcc tgagcttaaa
    13381 acttttgcgg cagccctcgc tcaggcagac cagctgggta tccccatcaa caatgttatg
    13441 aaggttcagt cagagcagct tagagaaacc cgccgccaga tggcaagaga aaagggcatg
    13501 aaggcgccta tcaagatgat gttccccatg gttggattca ttttccccgt tattttcatt
    13561 atcatattag gaccatctgt attaacaatt atggattcat tcttaataag caggtgagag
    13621 gcttgaaaac tctaagagca tacgcggcag acggcagaac ccttgtaaga aatgttgaat
    13681 tagcaaatac cttttttaca agacttaagg ggctgatgtt cagagaatcg ctgcctaatg
    13741 acacagggtt gctgatttat ccctgctgtc aaattcatac atacttcatg aaattcagta
    13801 tagatgtggt ctatctcaac agagatttca aggtagtttt cattgacaga aatattcagc
    13861 ccggtgtcag cgtcagaaga ataagagagg ctaaatgcgt tcttgaactg aatgccggag
    13921 aagccgacag gcttggcatt gcagttgatg atgtgttgac ttttgcataa acctgactta
    13981 taattttagt aagattttgt aaacattgct gcttaggggg acaatagatg tatcagaatc
    14041 ctgatgatag attatcggtt gacgccaata aagacggcaa ccaaaaagaa accgtgtctg
    14101 taaacaaagc agacggtcaa aactccgtac ttaatcttga tgacggttta tcatgcccca
    14161 tcaggacttc tgttactttt aaaaaagcac tcttcggcgg acttgacccc gacgaagttt
    14221 atgcttatat tgacgaacaa tttgagaatt acaacaatac ctgccaaatg tatgccggta
    14281 ggcttgaaga gttaaagaat aatataaccc taatttcccg tgagcgcgat tggctcaaaa
    14341 ttcagaacca ggacgcggtt aaaagggcag aggcggccga agaaaggctt atggcctctc
    14401 aaaaagcctt tgatgaggag ttggctttag ctcagcaggc tgaggaaaag gcccgcagag
    14461 aagccgagga agcctcaaga gcagccgctg aggcaaaggc tatggcgctt gaagccgaaa
    14521 gaaactcaaa gataatgtcc gagcagctca gcaaggcaaa agacgctcaa aggcgcgccg
    14581 aggaggagct gaacaaagcc cacactaaac tcaggctggc cgaggagcgc gcagttcttg
    14641 ccgaagaaaa aatgaaagaa gctcttaaag agcttgcaag ccaaaacgac agcaacgagc
    14701 tgcaggagaa atacaacgaa ttatcttcaa aatacaatca gctgttagaa gacagcagac
    14761 gcttaaaaga attcgaagca ggtcttgcac agagcgcaat tgaaaaggaa aacttacaga
    14821 aaaaaatcga atcgctcaac gctcaaatcg aaaagctcaa agaagaaaat cagcgtctta
    14881 gcggcgtcat aagcgaaagc aatcttcagc ttgaagcttt tgaaaagctc aaggttgatt
    14941 actcggcggt caacgcaagg ctttcggttg ctgaagctga gctaagctcc gtatccgttg
    15001 agctgagcaa aaagacggac gaaaacgaaa agctgcagtc aaccttggca gacctcagaa
    15061 tcagaatcgc ttctcttgaa cagcagaatg acaatctcga gaaggaaata tccgctctca
    15121 gagagaatct cagagttcag atgtacgaat tcgcccagaa gaaaaacgaa tacgaaaccg
    15181 cagccgctaa tgaaaggctg aaccttatta aactcataca ggtccattcc taccatctca
    15241 aacagtccga gatgcttctt aacgaactta gaacacaatt tagccacgct attgaaacct
    15301 ttaaggacct cgaaaaggaa gacgcagggc aagagtaaac cttgaagaat ataaggggag
    15361 gtttaacatg aaaaagaggc tgcgcagttt ttggaaagaa gaaagcggag ttgccgtaat
    15421 atttgtggca atctccatgg ttgttcttat gtcatttaca gctcttgccg ttgactacgg
    15481 aatggtatac tatcaccgct ccgagcttca aacggctctt gacagcgctg ctcttgcggc
    15541 tgctcaaaag ctccccgaca aagaagccgc aagacgaacc gcaattgagt atgtaagaaa
    15601 gaacggcata gaagttaaaa gtgatgatga gataatagta gattttctgc aaaacgatac
    15661 catagtaagg gtatcctcat cagtcgagac caaaacttct tttgcaagga tattcaatgt
    15721 agatactata aaaagtgtcg ctgtagctgc ggcccaaacg aaggaagtaa agcaagggca
    15781 acaaagcttt gaccacctgt tgttctcagg ctcgagagat gatcgtcttt tcctcgacag
    15841 tcaatttgaa atttttggca gtgttcatac aaataacaag ttttttttca gtccaagtaa
    15901 tggagttatt catggtgaag taagcgctgt tgttcctggc agctcataca acggtggctt
    15961 tagggctggc tcaattaatc caaatgccag ttatattcca atgcctgatt tttcagactc
    16021 tattgctaag tatgttccac ctttcccgac agaaacggca gaatccaact ttggttatgc
    16081 ggcaaataag ataaggggca ttcgcggata tgtttcgcct gcatatgtta acagtctgaa
    16141 tcagcagaat attacacttg agggcaaaac tgagtacagc gggccactta atataaaagc
    16201 ttggggttcc acaacaatca taggtgaaat ctatgtaaaa ggctccgcct ccttccaggg
    16261 caaacttatt atgaattccc ccaatgattt actgtgcgtt gacggtgact tgaacatagg
    16321 cggaggactt ttcggaaaag gcactcttat tgttgacggc gaccttacca tcagtggcgg
    16381 cggtccatac acctttgacg gcgatattta tgttgcagga aattttagaa ccaatgccgg
    16441 tcttaatgca acgggcaaaa tagtatgcaa aggtaatatg aacatcagtc aatcttttaa
    16501 tttttacgga ccttcctcta atattccagg tgtaatatac tgtgacggca atctatcagc
    16561 aaatttatat aaaggtaaag ctgacataat atgccaaggt acaatttctt ttaccggtaa
    16621 ttcagctgaa catgatggtc ttttatattc cgctaaacaa attaatgttt atactcgtct
    16681 tatttcgaac aacataacta tcctaagcaa agacgatatt acggtaaaca gtaattgtac
    16741 aatagctaaa ggtgttattt atgcaggaaa gaattttaac tgttatactg cgttagaggg
    16801 ttccgtctat gtttttgcag aaaacaattt gaattatagt ggatatgcaa ataataatac
    16861 acaattagat attgcattct atgccattaa tggaaatata agcattagtc tctccaaaac
    16921 taatgtcaca ggaataattt acgctcctaa gggcaatgtt catatgggag gatctataga
    16981 aatatatggt agtattatag ctaatagctt cacgggcagt tttagcaagt taacgattag
    17041 aagaaatgaa aatggcaatc ctcttgatgt aggcaaaact gtcaagcgaa cagttttggt
    17101 tcagtaattg acaatttttt aatcagataa tataattgtt cagtcaagta aatatttagg
    17161 cgatgattaa gcagaaaccg tgtatttcgg ctcaagagag gggcttttgg tgcgacgccc
    17221 tgccgaaaag cggtgagccg gcttatagcc tcttcgttaa tccgaagcgt tttccgcgtt
    17281 aaggaatttg agtggcgctt tgcgcaattt gggtggtacc gcggctttaa gtcgtcccaa
    17341 tttgtaaaag gcgcttattt tttgatttta tataactttg gaggaaattt ttaatgcagt
    17401 ggaccggact taacgaaatc agagaaaaat atctgagctt ttttgaaagc aagggacatt
    17461 taaggcttcc cagcttttca ttggttcccg aaggtgataa gagcctgctg ctaatcaatg
    17521 cgggcatggc tcctatgaaa aaatacttta cgggcgaagt tacccctccc agcaagaggg
    17581 ttaccacctg ccagaaatgc ataaggactc ccgatattga gcgcgtaggt aaaaccgcaa
    17641 ggcacggtac cttctttgaa atgctcggca acttctcttt cggcgattat ttcaaaagag
    17701 aggctatctc ctgggcatgg gagttctgca caaaggttct tgaaattcct gtggacaggc
    17761 tttacgccac ggtttatctc gaagatgacg aggcttatga tatctggaca aaagaaatcg
    17821 gcatcgacga aactcatata aagagaatgg gcaaggagga caacttctgg gagctggatt
    17881 caggtccctg cggaccctgc tccgagattt acttcgacat gggcgaggag ttcggctgcg
    17941 gttctcccga ctgcggcgtt ggctgcgact gcgaccgcta tgttgaattc tggaaccttg
    18001 tctttaccca gtttgaaaag gacagcgaag gcaactatac gccccttgct catccgaata
    18061 ttgatacggg catgggtctt gaaagaattg cctgcattat tcagggcgct ccgaatctct
    18121 ttgaggtcga caccgttaag ggcataatgg agcacataag cagtattgct ggcgtcaaat
    18181 accatgagga cccgaaaatg gatgtttcat taagagttat aaccgaccat atccgcagca
    18241 cggttatgat ggtcggcgat ggcattatgc cctcaaacga aggcagaggc tatgttttga
    18301 gacgcctttt aagaagagcc gcaaggcacg gcaggctttt aggcatagac agacccttcc
    18361 tttctgaact tgccgaaagc gttattaaat tcaacgccgg agcttatcct gacttaaccg
    18421 aaaagcacga ctatattatc aacgtcataa cccatgagga ggaga
//
LOCUS       DAONVQ010000021        18216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2251_length_18216_cov_4.018006, whole genome shotgun sequence.
ACCESSION   DAONVQ010000021 DAONVQ010000000
VERSION     DAONVQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 18216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18216
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2251_length_18216_cov_4.018006"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLD98_02530"
     CDS             <1..453
                     /locus_tag="PLD98_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HQA05158.1"
                     /translation="MAADMMDAGINAGKLNRILFETKSKSYAALEAMALATLTKHFSE
                     KCAIMHITLEMHEKSGTDETEIHALASLPRQIEGVLVGATIKEAEPGRYKVSIRTNPP
                     VNASEICQIFGGGGHKRAAGCEIHGTLSEVKKQLLDAIEPFVRDVDPE"
     gene            450..1337
                     /gene="truB"
                     /locus_tag="PLD98_02535"
     CDS             450..1337
                     /gene="truB"
                     /locus_tag="PLD98_02535"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQA05159.1"
                     /translation="MTGFCIIDKPEGLSSFQVSRALRRIFNEKKTGHIGTLDPMATGV
                     LPVAVGGATRFIPYIPDEDKEYIARFRLGIRTDTLDITGKVTDTSPVTATADEVEKIL
                     ERFRGRISQVPPMYSALSKDGVKLYKLARQGIETEREAREISVYSLELTNVLDYNEYE
                     IKVSCSKGTYIRSLIDDIGTALGTHAVMTYLRRTASNGFSIDEAVPLDALSEMPFDAA
                     AKTLISIDAAMSSYPEITVTEAQAKRFLNGGALSIDRLKNIKTDGLFRVYSPDRRFLG
                     LGEAGAELLNPKRVINADE"
     gene            1330..2238
                     /locus_tag="PLD98_02540"
     CDS             1330..2238
                     /locus_tag="PLD98_02540"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQA05160.1"
                     /translation="MNSDIKKTAIALGSFDGLHRGHMAVIEKAVRQKENGLRPIAVTF
                     KEHPQKYLNGSTPPLIFSGIVKERAFKDAGISLYELDFADIMRLTPEEFFEKILINKL
                     HAAFISCGYNYRFGINGTGDAELLKELCEKKGIEFCVAPKVEFENEPISSTRIRQAIL
                     AGDIEKANMMLGRPFSYCEKVVSGDHIGRKLGAPTINQFFPENHIIPKFGVYISKVEI
                     DGRFYRAVTNIGIRPTLELKVPRSETCIIDFSGDLYGRHIEVFLLKYLRPELIFEGLD
                     SLKGQIMSDIEKARLYEEPEIYGREI"
     gene            2235..2993
                     /locus_tag="PLD98_02545"
     CDS             2235..2993
                     /locus_tag="PLD98_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQA05161.1"
                     /translation="MKKFVSWNVNGLRAVMNKGFLDIFNEFDADAFCLQEIKLQEGQI
                     ELHLPGYYQFWNYAEKKGYSGTAIFTKDEPLSVFYGIGIPEHDTEGRVITLEYPEYYL
                     VTVYTPNAREDLSRLEYRVRWEDAFREYLLGLRVIKPVIFCGDLNVAHKEIDLKNPKA
                     NRGKAGFTDEEREKFTELLEAGFTDTFRYFYPDLEGVYSWWSYRFNARKNNAGWRIDY
                     FVTSEELNERLQHAAIHMDIFGSDHCPVSLIIKD"
     gene            3017..3736
                     /locus_tag="PLD98_02550"
     CDS             3017..3736
                     /locus_tag="PLD98_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQA05162.1"
                     /translation="MTVIWFAYFALYLIALSPSLLYAKILTKQGKIEKRNTLVRKHAK
                     KLIRRISRLAGVTFDIQGLENVPKDRNVVYIANHQGIFDVIALMMVPPEICGFLAKQE
                     LSKIPLARTWLEYMGCIFVNREKPREAVAALDMAEEKVRNGESFIIFPEGTRSKNGEV
                     GEFKNGAFRVAKNTGVPIVPVALEGTYKAWEEKKRISPAKVKVRVLPPIETEGMTREE
                     YKAIGAKVRELIVNAKPPRED"
     gene            complement(3895..4281)
                     /locus_tag="PLD98_02555"
     CDS             complement(3895..4281)
                     /locus_tag="PLD98_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQA05163.1"
                     /translation="MKYKVTLNGKTYEVEVTEKTAEVVSVSDAPAAPAQAAAPAPAAP
                     AAPAAPVAAAPTAEGETLPAPMPGTIVKVLAPAGTAVKEGDVLFILEAMKMENEIVSP
                     KTGTVAQVLVSEGASVSSGQPLCVIA"
     gene            complement(4317..4682)
                     /locus_tag="PLD98_02560"
     CDS             complement(4317..4682)
                     /locus_tag="PLD98_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05164.1"
                     /translation="MTIFNTIMAAIEFSADNSALEFAAIIAAAMLIAAAIIASKLGKV
                     AEAIEKLVSSPSATVPAPAHAAAPAAVAAAPAQRAVSHTVVLNGIDEETAAAIMAIVS
                     HESGIPLEKLEFKSIKEID"
     gene            complement(4695..6074)
                     /locus_tag="PLD98_02565"
     CDS             complement(4695..6074)
                     /locus_tag="PLD98_02565"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HQA05165.1"
                     /translation="MKVRFTETVLRDAQQSLIATRMPFEEFEGILPELDKAGYYSLEC
                     WGGATFDSCLRYLNEDPWERLRKIRKACPNTKLQMLLRGQNLLGYKHYPDDVVRMFVK
                     KSIENGIDIIRIFDALNDLRNIETAVDETLKNGAIASGTICYTTSPIHNLEAYVKLAK
                     DLENMGVNTISIKDMAGIIAPQDAYDLVKAIKENVKLPVIFHTHSTTGLGPISALKAV
                     EAGADVIDTAISSMAGGTSQPPTETLEYALRTAGYETGLDTAVLKKINDFFKDIRSKD
                     IESGLLNPIILSTETDALIYQIPGGMLSNLVAQLKAQNSLHLLDKVLEEVPRVREDLG
                     YPPLVTPMSQMVGVQAAINVLVGERYKNISNEIKAYIRGEYGKAPGKINEELIKKVLG
                     DEPMFTGRYADTLEPMFEKTKAELGSLARSDEDVLSYIAFPQQAESFFKRREAKEKGV
                     FEYSIQRID"
     gene            complement(6404..6682)
                     /gene="rpmA"
                     /locus_tag="PLD98_02570"
     CDS             complement(6404..6682)
                     /gene="rpmA"
                     /locus_tag="PLD98_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQA05166.1"
                     /translation="MLKINIQLFAHKKGVGSTKNGRDSESKRLGVKRGDGQSVHAGTI
                     IVRQRGTKIHPGNNVGRGSDDTLFALIDGKVKFERLDKTRKKVSVYPA"
     gene            complement(6676..6996)
                     /locus_tag="PLD98_02575"
     CDS             complement(6676..6996)
                     /locus_tag="PLD98_02575"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HQA05167.1"
                     /translation="MIKAVFTLKEDYVTGFSIKGHALYAEHGQDIVCAAVSSAAYMAA
                     NTVTEVFGAEADIKVDEGFFELKLKNPEKESCNVLKGLMLHINGLKEQYPTYIETTTE
                     VLSC"
     gene            complement(6996..7310)
                     /gene="rplU"
                     /locus_tag="PLD98_02580"
     CDS             complement(6996..7310)
                     /gene="rplU"
                     /locus_tag="PLD98_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQA05168.1"
                     /translation="MYAVIETGGKQYKVAVNDEIFIEKLGVEAEDEVVFDKVIAVVDG
                     DKLNVGAPYVEGAEVKATVLKNGKAKKITVFTYKPKKGYKRKLGHRQPYTKVRIDAIN
                     VK"
     gene            complement(7456..8706)
                     /locus_tag="PLD98_02585"
     CDS             complement(7456..8706)
                     /locus_tag="PLD98_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQA05169.1"
                     /translation="MKRAIVFAGGGSKGSYEFGAWSALRDLGQSFDIAAGTSIGSINA
                     GFYVQDDYEAAKEMWSTLSVDKIFANPVKLERSITALADQMDTIRPFLKAYINKKGAD
                     VTPFIENLNSYANEEKFFSSEVDYGLMTVAFPALIPVEITKSQIERGYLAKWIQASCA
                     CFPVFPTCEIDGKSYIDGGFYDNLPIATAFRLGAEEVVAIDLNPVSAHEAYALNPYVK
                     LIKPARDLGSFMDFDRASLDRNIRLGYNDTMKAFGKYHGRLYTFMGSDETMNRLEKLS
                     KDFTYEITRAETYPLKEKQRLFQRNAVNAPCTRRLADYSGKNSPTMLDFLISAIEITA
                     RLLAYPDDLLYSFESIFQMLRLDAYPIKEELERGLTEGIAAFSKLKREKNKEHRLLDL
                     KRSENDYEEIIATVLLKELFKLVE"
     gene            complement(8727..9740)
                     /locus_tag="PLD98_02590"
     CDS             complement(8727..9740)
                     /locus_tag="PLD98_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05170.1"
                     /translation="MKAINKEKFNKALKGRLAPICALIIAALVLLTALPYSVNAIRTR
                     NELPESSFEERGRADRIHFIGFENTDCVLIESNNRFALFYGGAFESAEKEKAEGISIA
                     DYLKLRAADGSGRVLLDYLICADSSYEKIEGLKSLIKDKGIYAKTIYFKRLDFSAVKM
                     DDELKETYYKVYEAILEKGTPIAPDSEEMLTLGDYKLHIIDAGDISDSSLNLLIKKGD
                     LSVFIAGNINSKDMKRLKNEIGEVTLYKAPFAGGKKDFPLSVAMKLKPDYIVFGGKPL
                     RFGPALMYRLCFAAGGSVYETYKEGGIVADLSTDEIRLCKIGQLGKIADSTTAAETES
                     SAK"
     gene            complement(9727..11316)
                     /locus_tag="PLD98_02595"
     CDS             complement(9727..11316)
                     /locus_tag="PLD98_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05171.1"
                     /translation="MFWVLYKSRMQSLFTAMFSVSRSKKKLSPAAYVGIAALVIYVFG
                     SLMFSNIAMFSAVGEQLIMAGMASTYFALIGLLLLGLGFFGSIFVAQVQLFQARDNDL
                     LLSMPIKPKTILASRIAMIITLVYIYQLIFVVPALFVYFKVAGMEIVNSLIFLILCFI
                     LPLFTVSISSLGGWAAAAASARMKNSRVISVLMVLLFIGGYMFISTRMNYYMQLIIQK
                     GETIVQGLKKVFFPMYLFAEASASGNILYALIFIAFCVLVFLIVSSLIARRFLKITTL
                     EQNAYKKKYKAKEIKARGKSSALFKKDLDYFTNTIGYLLNAGLGSVLMLLFAGVTVFG
                     GDSFKNEMFAALGDNFSMIAAMGIGFCAAMNIVTAPFISLESKFLDIVKSLPVSSMDV
                     LLSKLKLHLVVSQPFIFVSALVCSFGFKLSFTEFLLSLAFPAIINFMLGISGLLINLR
                     FPKFDWINETLVVKQSLSTFLAIFTGIGACLLPVLAFMLLSDYAEANLILLISCALYA
                     IVGIVLYRVLKTWGVKVYESY"
     gene            complement(11338..12054)
                     /locus_tag="PLD98_02600"
     CDS             complement(11338..12054)
                     /locus_tag="PLD98_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05172.1"
                     /translation="MLKIQNLTKTYGSKVAVDNLSLEINAGEIYGFIGHNGAGKTTTI
                     KACCGILAFDSGEIYIDGLSIKENPVEVKKRIAYIPDNPVLYEFMSGIKYLNFIADIF
                     ELPKDVRNREIEKYAELLELKDELHNPISSYSHGMKQKLALISALIHDPKLLFLDEPF
                     VGLDPKASHLLKVLMREKCDEGKAIFFSTHVLEVAEKLCDKVAIIKEGKLVVAGETAE
                     VIGQSTLESVFLELSEANKE"
     gene            complement(12077..12922)
                     /gene="folD"
                     /locus_tag="PLD98_02605"
     CDS             complement(12077..12922)
                     /gene="folD"
                     /locus_tag="PLD98_02605"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HQA05173.1"
                     /translation="MAELIDGKALAAEIRQAAALRVKSLNERGKTVGLAVIIVGNDPA
                     SRVYVNNKKKACAEVGIESFEYALSEDTTQDELLALIEKLNHDDRVDGILCQLPLPKH
                     LNADAVIDSISPDKDVDGFHAVNVGRLSLNKQGMRPCTPAGVMRMLQSKGIEVEGKHC
                     VVIGRSNIVGKPAAVMLINAGATVTVCNSKTKNLSEITRQADILIAAVGRPGFVTADM
                     VKDGAVVIDVGINRDENGKLCGDVDFDTVKEKASYITPVPGGVGPMTIAELIENTVLS
                     AELKA"
     gene            complement(12940..13995)
                     /gene="lgt"
                     /locus_tag="PLD98_02610"
     CDS             complement(12940..13995)
                     /gene="lgt"
                     /locus_tag="PLD98_02610"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQA05174.1"
                     /translation="MKFLADYTAVTFTGIDKVFDVPGVFVRFTMPIIGTPVEIRFYGL
                     IIFIGFTLAVLFGGRMAYKWKMSIDKMLDVLIYGTFGGIVGARLYYVAFEWNNYKDDL
                     LSIFKIWEGGLAIYGGLIGGIIAAYITCKFNKLNFYNLLDLAGMSFLIGQGIGRWGNF
                     TNQEAFGTNSDLPLGMWSEKVADYLARNQAKFLQQGIAVDPSKPVHPTFLYESVWCLV
                     GFLILYIVCKKARKFSGQIMLMYGVWYGTGRFIFEGLRTDSLYIPGTNLRTSQLLSLA
                     IVIICLVLLITKLIKYTKHPVPIEGVDYFPEEEKKEAEVAEVNTENGGAETAETAEKA
                     DDSGAENADAKESEAEI"
     gene            complement(14019..14756)
                     /locus_tag="PLD98_02615"
     CDS             complement(14019..14756)
                     /locus_tag="PLD98_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQA05175.1"
                     /translation="MSGHSKWSTIKRKKEKTDSQRAKIFTKVGREISVAVREGGPDPD
                     SNPKLRDAIAKAKANNVPNENIERIIKKASGEGSSENYEQIIYEGYGPGGVAVIVEAL
                     TDNRNRTAADVRHYFDKYGGKMGQSGSVMFIFEKQGIIIVDKENVSEEKLMEDALEAG
                     AVDFLTAEEYFDIRTAPNDVGAVREALEKMGYKLLSAEAEYVPNTEVKLEKEEDIKNM
                     LKMLEMFEDNDDVQNVWHNWANADDYE"
     gene            14937..15815
                     /locus_tag="PLD98_02620"
     CDS             14937..15815
                     /locus_tag="PLD98_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HQA05176.1"
                     /translation="MSFFDSLHPAVSLIFFLGVIFCAAFFINPVITLSALLGGLIFLC
                     ILKGRRAFFKDFVFILILILFSAALNPLFSAEGSTVILILFGRPYTLESLLYGIQAGA
                     MIGAVLCWFKCLSECFGSEKYLYLFSKALPKFSLILTIVFRYIPLIKEHIGKLRKAQK
                     ALGVKDNSLKNKLTLSMRIFGSAVVYSLESAVETGNSMKARGFGAANRTSYSLFRFSL
                     RDAVFSGVFFGLAIFSAMGFAFKGIEFEYYPNLGGISADTLSVFSYLSFFVLCLMPAI
                     CEIKENLKWRYLRLRV"
     gene            15788..17485
                     /locus_tag="PLD98_02625"
     CDS             15788..17485
                     /locus_tag="PLD98_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA05177.1"
                     /translation="MAIFEIKGLSFKYPESEGFALKNINLSINEGEMALLCGVSGCGK
                     TTLLRLLKKELSPFGETHGEILYDGAPLWELNEKRSAKEIGFVSQYSDGQIVTDKVFS
                     ELSFGLESLGENPQTIRLKVGEMASYFGIGEWFRKNTSELSGGQKQILNLASVIVTQP
                     RVLIFDEPTSRLDPNSASAFINILEKLNKELGITVIIAEHRLDELSPMADRILIMDNG
                     EIIVNTSPEKAGEALRKAAPAHPMLSALPAPVRLFELLKKQEGTNKALSGKTPPLTVK
                     AAREIIADCFAAEKAQRAKNANSHKDNESVLELKDVWFRYERDSEDVLRGVNLAVKKN
                     EAYFLLGGNGAGKTTTVNVAAGLLKPYRGKVKVKGKDIKGYKNGSLYKNLLALLPQDP
                     LTLFLEQKVSDDFRFVLKNQGLSETDCKERISKVSEKLKIENLLDMHILDISGGETQK
                     CAIAKMLLLSPEILILDEPTKGLDAAAKRDLGMLLKSLKSEGITIITVTHDIDFAAEF
                     ADRCALLFDGAVAAEGAAREFFGENFFYTTAACRISKGFIDGVLTVEELANNLRLKD"
     gene            17524..18210
                     /locus_tag="PLD98_02630"
     CDS             17524..18210
                     /locus_tag="PLD98_02630"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HQA05178.1"
                     /translation="MKKKIPIYLLIFIAAALLVTGVFRFAKDNYALITLIMAVLSMLP
                     LFLSFEKKKADTKLLAVLAALIAVSVLSRFIFFAVPFFKPVTAMIIIIAMYLGSDAGF
                     AAGAATALISNFYFGQGPWTAFQMFSWGLIGFAAGLLAKRLKKSTALLLVYGALSGVF
                     FSLLSDLWSVLWYDGGFNLKRYIALVTSAAGITLTYAVSNVVFLLIMKNVVGGAIERV
                     KLKYGIGIDS"
BASE COUNT         4548 a         4312 c         4427 g         4929 t
ORIGIN      
        1 atggcggctg acatgatgga cgcgggcata aacgctggca agctcaatag gatactcttt
       61 gagaccaagt ccaaatccta cgccgcactt gaggctatgg cgcttgccac gctcacaaag
      121 catttctcag aaaagtgcgc cattatgcac atcacccttg agatgcacga aaagagcggc
      181 accgatgaaa ccgagattca tgcccttgcc tcactccccc gccagattga gggtgtgctt
      241 gtgggcgcca caattaaaga ggctgagccg ggaagatata aggtgtcgat cagaacaaat
      301 ccccccgtca acgcttctga aatatgccag attttcggcg gcggcggaca taagagggcc
      361 gcgggctgcg agattcacgg cactctgagc gaagttaaaa agcagctttt agacgccata
      421 gagccctttg tgagggatgt ggaccccgaa tgacgggatt ttgtataatc gacaaacccg
      481 aggggctttc ctcttttcag gtttcaaggg ctttaaggcg tatcttcaac gaaaaaaaga
      541 cggggcatat aggcaccctt gacccgatgg ctacgggggt tctgcccgtg gctgtgggcg
      601 gcgccacaag gtttattcct tatatccccg atgaggataa agagtatata gcaaggttca
      661 ggctcggaat cagaaccgac acccttgaca taacgggcaa agtcacggat acatctcccg
      721 taacggccac tgccgatgaa gtggaaaaga ttttggaaag attcaggggc aggatttctc
      781 aggttccgcc catgtactcc gccctgtcaa aggacggagt aaaattatat aagctggcgc
      841 ggcagggcat agaaaccgaa cgggaggcaa gggaaataag cgtttattcc cttgagctga
      901 ccaatgtgtt ggattataat gaatatgaaa taaaggtttc ttgttctaag ggcacatata
      961 tcaggagcct tattgacgac ataggcaccg cccttgggac acacgcggtt atgacatatc
     1021 ttcgccgcac cgcctcaaac ggctttagta tagacgaggc ggtacctctt gacgctctgt
     1081 ccgaaatgcc ctttgacgcc gccgcaaaga cgcttataag cattgacgcc gctatgtctt
     1141 cttaccccga aatcaccgta acggaggctc aggcgaaacg attcttaaac ggcggagctt
     1201 taagtattga caggctaaag aatatcaaaa cagacgggct tttcagggtc tactcccccg
     1261 acaggcgctt tttggggctt ggagaggcag gggcagagct actgaatccg aagcgggtga
     1321 ttaacgctga tgaatagcga tataaaaaag accgccattg ccctgggctc ctttgacggg
     1381 ctgcacaggg gtcacatggc ggttatagaa aaggccgtaa ggcaaaaaga gaacggtctc
     1441 cggccaatag ccgttacctt taaagagcac cctcaaaagt acttaaacgg cagcactcct
     1501 cccctgattt tctcagggat tgtaaaggaa agagctttta aagacgccgg aatcagcctt
     1561 tatgagctgg atttcgccga cataatgcga cttactcccg aggagttttt tgaaaagata
     1621 cttataaaca agctccacgc cgcttttatt tcctgcggtt acaactaccg tttcgggata
     1681 aacggcacgg gcgacgccga gcttctgaag gagctttgcg agaaaaaagg cattgaattc
     1741 tgcgtcgccc ccaaagtaga gttcgaaaac gagcctataa gctccacaag aataaggcag
     1801 gcaatactcg ccggcgacat tgaaaaagcg aatatgatgc tgggcagacc cttttcctac
     1861 tgcgaaaaag tggtcagcgg cgaccatatc ggaaggaagc tgggcgctcc gacaataaat
     1921 cagttcttcc ccgaaaatca tataatccca aagttcgggg tttatatttc aaaggtagag
     1981 atagacggca ggttctacag ggcggtgacc aatatcggca tcaggcccac gctggagctt
     2041 aaagtccccc gcagcgaaac ctgcattata gatttctcag gcgaccttta cggtaggcac
     2101 attgaggttt ttctgcttaa atacttaaga cccgagttga tttttgaagg tcttgacagc
     2161 cttaaaggtc agattatgag tgatattgaa aaggcaaggc tttatgagga gcctgaaatc
     2221 tacgggagag agatatgaag aaatttgttt cctggaatgt aaacgggctg agggcggtaa
     2281 tgaacaaggg ctttcttgat attttcaatg agtttgacgc ggacgccttc tgccttcagg
     2341 agattaagct gcaggaaggg cagattgagc tgcaccttcc cggctactat cagttctgga
     2401 actacgccga gaaaaagggc tattcgggca ccgcgatttt cacaaaggac gagccccttt
     2461 ccgtattcta cggcataggc atccccgagc acgacactga aggacgggtc ataacccttg
     2521 aatatcccga atactatctt gtaacggtat atacgcccaa cgccagggag gacctttcca
     2581 gactcgaata cagggtaagg tgggaggacg ccttcaggga gtacttactc ggactcagag
     2641 tcataaagcc agtgattttc tgcggcgact taaatgttgc ccacaaggaa attgacctta
     2701 aaaaccccaa ggcaaacagg ggcaaggcgg gctttaccga tgaggaaagg gagaagttta
     2761 cggagctctt ggaggcgggc tttaccgata ccttcaggta cttttatccc gaccttgagg
     2821 gggtttattc ctggtggtca taccgtttta acgcaagaaa gaacaacgcg gggtggcgta
     2881 tcgactactt tgtgacctct gaggagctga acgaaagact tcagcacgcg gcaatacaca
     2941 tggacatttt cggctccgac cactgccccg tttcccttat aataaaagac taattgcggc
     3001 ggaaggattg atttttgtga ctgttatctg gtttgcttac tttgctctgt atcttatagc
     3061 tctgtcgccc tcccttttat acgcgaagat tctcacaaag cagggcaaaa tcgaaaagag
     3121 aaacaccctt gtaagaaagc acgcaaagaa acttataagg cgcattagcc gtcttgcggg
     3181 cgtgacattc gatattcagg gtcttgagaa tgtgccgaaa gacagaaatg tggtttatat
     3241 tgcgaaccat cagggaatct ttgatgttat cgccctgatg atggtgccgc ccgagatttg
     3301 cggctttctg gcaaaacagg agctttctaa gattcccctt gccaggacct ggctcgaata
     3361 catgggctgc attttcgtaa acagggaaaa gcccagagag gcggttgccg cccttgacat
     3421 ggctgaggag aaggtcagaa acggcgaaag cttcattatt ttccccgagg gcacaaggtc
     3481 aaagaacggc gaagtgggcg agttcaagaa cggagccttc agagtcgcga agaatacggg
     3541 cgtgcctata gtgcccgttg ccctagaggg cacatacaag gcatgggagg agaaaaaaag
     3601 gatttctccc gctaaggtaa aagtcagagt gctgcctccc attgaaacag agggcatgac
     3661 aagagaggaa tacaaggcca taggcgcaaa agtcagggaa ctgattgtaa acgccaagcc
     3721 accgagagaa gactaaaaat aggcagggaa acctgccttt tttgtttggt tttttagggg
     3781 ggccttagtt tcagacaaaa agaaaaggca ggtctaagcc tgcctaaatg aacaatatta
     3841 agaggtcggc ggcaaaaccg ctgaccgata ttttgcgttt ttacccgctt ttttctatgc
     3901 gattacgcag aggggctgac ctgaggatac gctggcgccc tcggatacaa gcacctgagc
     3961 gactgtgcct gtcttggggg atactatctc gttctccatc ttcatagcct caaggataaa
     4021 gagcacatcg ccctccttaa cagcggtgcc tgcgggggca agaaccttta caatggtgcc
     4081 cggcatgggg gcgggcaggg tctcgccttc agcggtcggc gctgcggcga cgggagccgc
     4141 gggcgctgcg ggagcagcgg gtgcgggagc tgccgcctga gcgggcgctg cgggagcgtc
     4201 gcttacgctt actacctctg cggttttttc ggttacttcc acttcgtatg tcttcccgtt
     4261 aagggttact ttatatttca ttgtattagt cctttccgaa aaaatttaat aggctcttag
     4321 tctatttcct tgattgactt aaactcgagt ttttccagcg gaattccgct ttcgtggctt
     4381 acaatcgcca taatagccgc tgcggtttcc tcatctatgc cgttcagcac tacagtatga
     4441 gatactgccc tttgagcggg agctgcggcg actgccgcgg gagctgccgc atgagcgggc
     4501 gcgggaaccg tagctgaggg agaggaaaca agcttttcta tcgcctctgc cactttgccg
     4561 agtttgcttg ctataattgc cgcggctatc agcatagcgg cggcgatgat tgccgcaaat
     4621 tcaagggcgg agttgtcggc agaaaactct attgctgcca taatagtatt aaaaattgtc
     4681 acgccggctc ctccttaatc tatcctctga attgaatatt cgaacacgcc tttttccttt
     4741 gcctctctcc tcttaaagaa gctctcggcc tgctgcggga aggctatgta tgatagcaca
     4801 tcctcgtcgg agcgggcaag actgccaagt tctgcctttg tcttttcgaa catgggctcc
     4861 agggtgtcgg cgtatctgcc ggtgaacatg ggctcgtcgc ccagaacttt ctttataagc
     4921 tcttcgttta tcttgccggg ggctttgccg tattcgcctc tgatgtatgc cttaatttcg
     4981 ttagaaatat tcttgtatct ctcgcccaca agcacattga ttgccgcctg aacgccgacc
     5041 atctggctca tgggagttac aaggggcgga taacctaaat cctctctgac tctcggaacc
     5101 tcctcaagaa ctttgtcaag gagatggaga gaattctgag ccttgagctg tgccacaagg
     5161 tttgaaagca tgccgccggg tatctggtaa atcagcgcgt ctgtttcggt ggaaaggata
     5221 atgggattta aaagtccgct ttcaatatcc tttgacctaa tatccttaaa gaagtcgttg
     5281 attttcttaa gtacagccgt gtcaagacct gtttcgtagc ccgccgttct gagggcgtat
     5341 tccaaagttt cggtgggggg ctgagatgtg ccgcccgcca tgctggaaat ggctgtgtct
     5401 ataacatcgg cgcctgcctc tactgcctta agagcggaga tggggccaag acccgttgtg
     5461 ctgtgggtgt ggaatataac gggcagcttt acattctcct taatggcctt tacaaggtcg
     5521 taggcgtcct gcggggcgat aatgcccgcc atgtctttta tgctgatggt attgacgccc
     5581 atgttctcaa ggtcttttgc cagcttcaca taagcctcga gattatgtat ggggcttgtg
     5641 gtgtagcaga ttgtgcccga tgctatggcg ccgtttttaa gcgtttcgtc aacggcggtc
     5701 tcgatgtttc tcaggtcatt gagagcgtca aagattctaa ttatatcaat accgttctcg
     5761 attgactttt tgacaaacat cctgaccaca tcatcaggat aatgcttata gcccaagagg
     5821 ttctgacctc ttaaaagcat ctgcagcttt gtattggggc aagcctttct gattttgcgc
     5881 agcctctccc acgggtcctc gttaaggtac ctaagacatg agtcaaaggt tgcgccgccc
     5941 cagcactcca atgaataata gcccgcctta tcaagctcgg gaagtatgcc ctcgaattct
     6001 tcaaagggca tacgggtggc aatcaaagat tgctgagcat ctctcaagac ggtttccgta
     6061 aatctgactt tcatatctat ctcctgtaag gattgttaaa aatgttctta aagtgaacct
     6121 tggcggatat tagtttattt tgtcattttt gaacatttaa taaattaatc caccaaattc
     6181 acccatcata tcatacccta atatttaaaa aatttcaaga tatttgatag tttctgatta
     6241 aatttttgaa attcttttat tttctcttta attctctttt gtcaaattgg ttaaattaat
     6301 aacaaattag aggacagaac ctgtcctcca tgagcttttc ttcaaaaagc taaagaccgc
     6361 accttatgat gcggcctgaa tggtcttttt gtttttttaa gttttaagcg ggatagacgc
     6421 tgaccttctt gcgggtcttg tcaaggcgct caaactttac cttgccgtcg atgagggcaa
     6481 agagtgtgtc gtcagagcct ctgccaacat tgttgccggg gtgaatcttt gtgcctctct
     6541 ggcgaacgat gattgtgccg gcatgaacgg actgaccgtc tccgcgctta acgccaaggc
     6601 gctttgactc ggagtcgcgg ccgttttttg tggagccgac gcccttttta tgtgcaaaca
     6661 gctgaatatt tatctttaac atgataaaac ctccgttgta gtttcaatat aagtggggta
     6721 ctgctcttta aggccgttta tgtggagcat aagtcccttt aagacattgc agctttcctt
     6781 ttcggggttt tttagcttca gctcaaaaaa cccttcgtct acttttatat ccgcctcggc
     6841 gccgaaaacc tctgtcacgg tattcgctgc catatacgcc gcggaagaaa cagccgcgca
     6901 gactatatcc tgcccgtgct ctgcgtaaag ggcgtgacct ttaatggaaa agcctgttac
     6961 atagtcttct ttaagggtaa aaacagcctt aatcattatt ttacattaat ggcatcgatt
     7021 ctgacctttg tgtagggctg cctgtgaccc agcttacgct tatagccctt cttgggcttg
     7081 taggtgaaaa cggtaatctt ctttgccttg ccgttcttta aaacggtagc ctttacttcc
     7141 gcgccttcaa catagggagc gccgacattc agcttgtcgc cgtcaacaac agctataacc
     7201 ttgtcaaata ctacttcgtc ttctgcttca acgccaagct tttcgataaa aatttcgtca
     7261 tttacggcaa ccttgtactg tttgccgcct gtttcaatta ctgcgtacat aattaccttc
     7321 ctattttcca gactcgctac ggcaggtgga gcaaagctct ctttaacata cctgcaatat
     7381 gcggcaaagc agattgtacc attgagatac ctttttgtca agcatttttt cctttttgtc
     7441 gctctttgga aaaaattact ccaccagttt aaaaagctct tttaaaagca cggtggctat
     7501 gatttcttca tagtcgtttt ccgaacgctt gaggtccaat agcctatgct ccttgttctt
     7561 ttcccttttg agctttgaga aggcagctat gccctcagtc agaccccttt ccagctcttc
     7621 ctttatggga taggcgtcga gcctcagcat ttggaaaata gactcgaagg aataaagaag
     7681 gtcgtcgggg tatgcaagca gccttgctgt gatttctatg gcggagataa gaaaatcgag
     7741 catagtaggg ctgtttttgc ccgaatagtc ggcaaggcgc cttgtgcatg gagcgtttac
     7801 cgcattgcgc tggaagagcc tttgcttttc ttttaagggg taggtttccg ccctggttat
     7861 ctcataggta aaatcctttg agagtttttc aagacgattc attgtttcgt cgctgcccat
     7921 gaaagtgtag agcctgccgt gatattttcc gaatgccttc atggtgtcgt tgtagccgag
     7981 ccttatattc ctgtcaaggc tcgccctgtc aaagtccata aaggaaccca aatcccttgc
     8041 gggctttatg agcttaacat agggatttaa ggcgtatgcc tcatgggcgc tgacggggtt
     8101 taaatctatt gcaacgacct cctccgcgcc gagtcgaaat gctgtggcga tggggaggtt
     8161 atcgtaaaat ccgccgtcaa tatagctttt gccgtctatc tcgcaggtgg ggaatacggg
     8221 gaagcaggcg caggacgcct gaatccactt tgcgagatag cctctttcta tttgggattt
     8281 ggtaatctca acgggtatga gcgccgggaa cgcgacggtc ataaggccgt agtcaacctc
     8341 ggaggagaaa aacttttcct cattggcgta gctgttgaga ttctcgataa agggggtgac
     8401 atccgcgccc tttttgttta tgtaagcctt taagaaggga cggatagtgt ccatctggtc
     8461 ggcaagggct gttatggagc gctccagctt tacgggattg gcgaaaatct tgtcaacgct
     8521 taaggtgctc cacatctcct ttgcggcctc gtagtcgtcc tgaacataaa agcccgcgtt
     8581 aatggagcct atggatgttc ccgcagctat gtcaaaactc tgtcccaaat cacgaagcgc
     8641 cgaccatgcg ccgaactcat aagagccttt tgagccgccg ccggcaaaaa caatagctct
     8701 tttcattttc catcgcctct tagactttat tttgcgctgc tttcggtttc agccgcagtg
     8761 gtgctgtcgg caatcttgcc gagctgaccg attttgcaga gccgaatttc gtcagtgcta
     8821 agatcagcca cgattccgcc ctctttataa gtttcataaa ccgagccgcc cgccgcaaaa
     8881 cacagcctat acattagagc gggcccgaat ctcaagggct tgccgccaaa gactatatag
     8941 tcgggcttga gtttcatagc gacactcaag gggaaatcct tttttccgcc ggcgaaggga
     9001 gctttatata gcgttacctc gccgatttcg tttttgagcc ttttcatatc ttttgagtta
     9061 atattgcccg caataaaaac ggacaaatcg ccttttttga taaggagatt aagagagctg
     9121 tcggaaatgt cgcccgcgtc tatgatatgg agcttataat cgcccaaagt cagcatttcc
     9181 tcgctgtcgg gggcaatggg cgtacccttt tcaagtattg cctcatagac cttgtaatat
     9241 gtttctttca gctcgtcgtc catttttacg gcgctgaaat ccagtctttt aaagtatatg
     9301 gttttggcgt aaatgccctt atcttttata aggcttttaa gcccctcgat tttttcatag
     9361 gagctgtcgg cgcagataag ataatccaaa agcactctgc cgcttccgtc cgcggctctg
     9421 agctttaaat aatccgctat tgatatgcct tctgcctttt ctttttcggc gctttcgaat
     9481 gctccgccgt aaaagagggc aaagcggttg ttggactcaa taagcacgca gtcggtgttt
     9541 tcaaaaccga taaaatgaat cctgtctgcc ctgccccttt cttcaaagga gctttcgggc
     9601 agttcgtttc ttgtgcggat ggcatttacg ctgtacggga gagcggtcaa aagcactaaa
     9661 gcggcaatta taagcgcaca aatcggagcc aatctgccct ttaacgcctt attaaatttc
     9721 tccttgttaa tagctttcat agacctttac tccccatgtt ttgagcactc tgtaaagcac
     9781 gattccgaca atggcgtaga gagcacagct tataagcaga ataagatttg cttccgcata
     9841 gtctgaaagc agcatgaacg ccaaaacggg caggagacat gcgccgattc ccgtaaaaat
     9901 cgcgaggaag gtgctgagac tctgctttac cacaagggtt tcgtttatcc agtcaaactt
     9961 ggggaatcta agattaataa gaagaccgct gatgcccagc ataaagttta ttattgccgg
    10021 gaacgcaagg ctgagaagaa attcggtgaa actcagctta aatccgaacg agcagaccaa
    10081 agccgaaacg aatataaagg gctggcttac gaccagatgg agcttcagct ttgaaagaag
    10141 cacatccatg gagcttacgg gcagggattt tactatgtcc aagaacttgc tttcaaggga
    10201 aataaacggg gcggtgacaa tgttcatcgc agcgcagaag ccgattccca tggccgctat
    10261 cattgagaaa ttatcgccca aagcggcaaa catttcgttc ttaaagctgt cgccgccgaa
    10321 aaccgtaacg cccgcgaaaa ggagcatcag cacagagccg agtcccgcat tcaaaaggta
    10381 gcctattgtg ttggtaaagt agtctaaatc ctttttgaaa agggctgagc tcttgcctct
    10441 tgcctttatc tcctttgcct tgtacttctt tttatacgcg ttctgttcca aagtggtaat
    10501 tttgaggaat cttcttgcaa taagcgagct tactataaga aataccagaa cgcagaaggc
    10561 gataaagatg agagcgtata ggatattgcc ggatgcgctg gcctcggcga aaagatacat
    10621 ggggaaaaag acttttttaa gaccctgtac tatcgtttca cccttttgga taatcagctg
    10681 catatagtag ttcattcttg tggaaatgaa catatatccg ccgatgaaca aaagaaccat
    10741 aagaacgctg attaccctgc tgtttttcat ccttgcggag gcggctgccg ctgcccatcc
    10801 gccaagggag cttatcgaca ccgtaaacaa tgggagaata aagcaaagta taaggaatat
    10861 caggctgtta acaatctcca tacccgcaac cttaaaatag acaaagagcg cgggcaccac
    10921 aaaaatgagt tgatagatat atacaagggt gataatcatt gcgattcggc ttgccaaaat
    10981 agtcttaggc tttatgggca tggacaaaag aaggtcattg tcccttgcct gaaagagctg
    11041 gacctgagct acgaaaatgc tgccgaaaaa gcccagtccc aagagcaaaa gacctattaa
    11101 ggcaaagtat gtgcttgcca tgcccgccat tataagctgt tcgcctacgg ctgagaacat
    11161 ggcaatatta gagaacatca gagagccgaa aacatatatg accaaagccg cgatgcccac
    11221 ataagccgcg ggggacagtt tctttttaga cctcgacacg ctgaacatcg cggtaaacag
    11281 cgactgcatt cttgatttat aaagaaccca gaacatttaa ttcagtcctt tcccgtatta
    11341 ctctttattt gcctcggaaa gctcaaggaa aacgctctcc aaggtggact gacctattac
    11401 ctccgcagtt tcgcccgcta ctaccagctt tccttccttg attatggcaa ccttgtcgca
    11461 gagcttctcc gcaacctcta gaacatgggt ggagaagaat attgccttgc cctcatcgca
    11521 cttttcacgc ataagaactt ttaagaggtg ggaggcttta gggtcaagtc ccacaaaggg
    11581 ctcgtcaagg aacagaagtt ttgggtcgtg aataagagcg gagataaggg cgagcttttg
    11641 cttcattccg tgggaatatg agctgatggg gttatgcagc tcgtctttaa gctccaaaag
    11701 ctcggcatac ttttcgattt ctctgtttct tacatctttg ggaagctcga agatatcggc
    11761 gatgaaattc agatatttaa tgcccgacat aaattcgtac agaacgggat tgtcgggaat
    11821 gtatgctatt ctttttttga cctcaacggg gttttctttg attgaaagcc cgtcgatgta
    11881 gatttcgccg gaatcaaagg ccagaatgcc gcagcaggcc ttgattgtgg tcgtcttgcc
    11941 ggctccgttg tgtcctataa agccgtagat ttcgcccgcg ttaatctcaa ggctcagatt
    12001 gtccacggcc accttgctgc cgtaggtttt cgtgaggttc tgaattttaa gcaagaaaat
    12061 caccctttat atgtttttaa gccttcagct ccgctgagag gacagtgttt tctattagct
    12121 ccgcaattgt catggggcct acaccgccgg gaacgggggt gatgtaagac gccttttctt
    12181 ttacagtgtc aaagtccaca tcgccgcaga gcttgccgtt ttcgtccctg ttaatgccca
    12241 catcaataac gacggctccg tctttgacca tgtcggctgt gacgaatccg ggtctgccga
    12301 cggcggcgat aaggatatca gcctgtcttg taatttcgct gagattcttt gtctttgaat
    12361 tgcatacggt cacggtcgcg cccgcgttga tgagcataac agcggcgggc ttgccgacta
    12421 tattgctcct gcctataacg acgcagtgct tgccttcaac ctcgattccc ttgctttgaa
    12481 gcatgcgcat gactcccgcg ggggtgcacg ggcgcattcc ctgcttgtta agagagagcc
    12541 tgcccacatt tacggcgtga aagccgtcca catccttgtc gggagaaatg ctgtcaataa
    12601 ccgcgtccgc gtttaaatgc ttcggcagcg ggagctgaca gagtatgccg tcaaccctgt
    12661 cgtcatggtt cagcttttct atgagcgcca aaagttcatc ttgagtagtg tcttcgctga
    12721 gcgcgtattc aaaggactct atgccgactt cggcgcaagc cttctttttg ttgttcacat
    12781 agactctgga tgcggggtcg ttgcccacta ttatgaccgc aagacccaca gttttgccgc
    12841 gctcgtttag actttttact cttaaagccg ccgcctgtct tatttcggcg gcaagtgcct
    12901 ttccgtcaat taattccgcc atttaaatgt tttctccttc tatatttctg cttcgctttc
    12961 cttagcgtca gcattttcag cgccgctgtc atccgctttt tcggcagttt cagcagtctc
    13021 tgcgccgccg ttctcggtgt ttacttcagc gacttcggct tccttctttt cttcctcggg
    13081 gaagtagtca acaccctcaa tgggaactgg atgctttgtg tacttaatta gcttagtgat
    13141 aagaagcaca aggcagataa taacaatagc gagggataaa agctgagagg tcctaaggtt
    13201 cgtgccgggg atataaaggc tgtcggttct gagaccctca aatatgaatc tgcccgtgcc
    13261 gtaccatacg ccgtacatga gcataatctg accgctgaac tttcttgcct ttttgcagac
    13321 tatgtagaga atcagaaagc ccacaaggca ccaaacggac tcataaagga aggtggggtg
    13381 gacgggcttt gaggggtcaa ccgcaatgcc ctgctgcaaa aactttgcct gattgcgggc
    13441 aaggtaatca gcgacctttt cgctccacat tccaaggggc aaatctgagt tggtgccgaa
    13501 cgcctcttga tttgtaaagt tgccccagcg tcctatgccc tgccctataa gaaagctcat
    13561 gcccgcaagg tcaaggaggt tgtagaaatt cagtttattg aatttgcaag ttatataagc
    13621 cgctataatg ccgccgatta agcctccgta aatggcaaga ccgccttccc atattttgaa
    13681 tatggaaaga aggtcgtcct tgtagttatt ccactcaaag gcaacataat atagccttgc
    13741 tccgacaatg ccgccgaatg tgccgtatat cagcacatcc agcattttgt cgatgctcat
    13801 cttccactta taggccattc tgccgccgaa aagaacggca agggtaaaac ctatgaatat
    13861 tatcagcccg taaaagcgaa tctcaacggg ggtgccgata atcggcattg taaagcgcac
    13921 aaacacgccg ggaacatcaa aaaccttatc tatgcccgta aaggtgaccg ctgtgtaatc
    13981 agccaaaaac ttcatgttaa acttcctttc ggatgccctt attcgtaatc gtccgcgtta
    14041 gcccagttat gccagacatt ctggacatcg tcgttgtcct cgaacatctc aagcattttg
    14101 agcatgttct ttatgtcctc ttccttttcg agctttacct cggtattcgg gacatattcc
    14161 gcctcggctg aaaggagctt gtagcccatt ttctcaaggg cttctctgac ggcgcccaca
    14221 tcgttcgggg cggtgcggat atcgaaatac tcttccgcag tgaggaaatc cacggctccc
    14281 gcttccagag cgtcctccat cagcttttct tcgctgacat tttccttgtc gacaatgatg
    14341 atgccctgct tttcaaagat gaacataacg ctgccggact gacccatctt gccgccgtat
    14401 ttgtcaaagt aatgccttac atccgcggcg gtgcggttgc ggttgtccgt cagagcctcc
    14461 actataacgg cgactccgcc ggggccgtag ccttcgtata taatctgctc gtagttttct
    14521 gagctgccct cgcccgaggc cttctttatg attctttcaa tattctcatt gggaacattg
    14581 tttgcctttg cttttgctat agcgtccctg agcttggggt tgctgtcagg gtcgggaccg
    14641 ccttcacgga cggccacgga tatttcacgg cctacctttg tgaaaatctt agccctttga
    14701 ctgtcggtct tttctttctt tctcttaatg gtgctccatt tggaatggcc tgacatatat
    14761 ttcttccttt caagaaaata taaacattag tattttatca ctctaaaccc cagtagtcaa
    14821 cataagcctg tctgctaaaa gcggcaaaac ccgcgtagct tctgagtttt ccgaattatt
    14881 gactttgaaa ataataaaga ttataattat tgttaagcta atgctggggt ggtttaatga
    14941 gttttttcga ttccctgcac cctgcagtat ccttaatttt tttccttggc gtaatatttt
    15001 gcgccgcgtt ttttataaat cccgttataa ccctctccgc ccttttggga gggcttattt
    15061 tcctttgcat cttaaagggc agacgggcgt tttttaagga ttttgttttt attctgatac
    15121 tgattctttt ttccgcagcg ctgaatcctt tgttttcggc tgagggaagc accgtaatat
    15181 taattttatt cggaagaccg tacacccttg agtcgctgct ttacggaatt caggcagggg
    15241 cgatgatagg agcggtgctg tgctggttta agtgcctttc ggaatgcttt ggctctgaaa
    15301 agtacctata tctcttttca aaggcgctgc cgaaattttc gctgattttg acgatagtat
    15361 tcagatacat tcccttgata aaagagcata tcggaaagct cagaaaagcg caaaaggctt
    15421 tgggagtcaa agacaacagc ttaaaaaata agctgacgct ctcaatgaga attttcggct
    15481 ccgccgtggt ttactccctt gaaagcgcgg tggaaacggg aaattcaatg aaggcaaggg
    15541 gctttggcgc cgcaaacagg acaagctatt cactgtttcg ctttagcctt cgcgacgctg
    15601 ttttttcagg cgttttcttc gggctggcaa tcttttcggc tatgggcttt gcctttaagg
    15661 gcattgaatt tgagtattat ccgaatttag gcggcataag tgccgatact ctttcagtgt
    15721 tttcttatct ttcgtttttt gtcctttgcc taatgcccgc tatttgtgag attaaggaga
    15781 accttaaatg gcgatatttg agattaaggg tctgagcttt aaatatcccg aaagcgaagg
    15841 ctttgcctta aagaatataa acctctcaat taatgagggg gaaatggcgc ttttgtgcgg
    15901 agtgtcgggc tgcggcaaga ccactctttt aaggctgtta aaaaaggagc tttcgccctt
    15961 tggggaaact catggggaga ttctttacga cggcgcgcct ttatgggagc tgaatgaaaa
    16021 gcgaagcgca aaggaaatag gctttgtgag ccagtattcc gacggtcaga tagtgacgga
    16081 caaagttttc tctgagctct ctttcggact cgaaagcctt ggagaaaatc cgcagacaat
    16141 aaggctcaag gtcggggaaa tggcaagcta tttcggaatc ggcgaatggt tcagaaagaa
    16201 taccagcgag ctgtcgggcg ggcaaaagca gatactcaat ctcgcctctg tcatagtaac
    16261 gcagccgagg gttctgattt tcgatgagcc tacatcaagg cttgacccaa actcagcgtc
    16321 ggcgtttata aatatccttg aaaagctgaa caaagagctc ggcattaccg taataatcgc
    16381 agagcaccga cttgatgagc tgagccccat ggcggacaga attcttatta tggataacgg
    16441 ggaaattatc gtaaacactt ctcccgaaaa ggcgggggag gctctgcgca aagcagcccc
    16501 cgctcacccg atgctctccg cactgcccgc tcccgtaaga ctgtttgagc tgttaaagaa
    16561 gcaggagggg acaaacaagg ctctaagcgg caaaacccct cccctcacag taaaggctgc
    16621 aagggagata atcgccgatt gctttgcggc tgaaaaggct caaagggcta aaaacgcaaa
    16681 ctcccataaa gacaatgaga gcgttctgga gcttaaagat gtctggttca gatacgaaag
    16741 agactccgag gatgttttaa ggggcgtaaa tctcgccgtc aagaaaaatg aggcatattt
    16801 cttgctgggc ggcaacggcg cgggaaagac tacgaccgta aatgtcgccg cggggctttt
    16861 aaagccgtac aggggcaagg taaaggtaaa aggcaaggat attaaaggct ataaaaacgg
    16921 aagtctatat aaaaatcttc tcgctctcct cccccaggac ccgctcactc tttttttgga
    16981 gcaaaaggtt tcggatgatt ttcgctttgt cttaaagaat caggggctca gtgaaacgga
    17041 ctgcaaggaa agaataagta aggtttcgga aaaactgaaa atagaaaacc ttttagacat
    17101 gcacatcctt gatatttccg gcggggagac gcagaaatgc gccattgcca aaatgctgct
    17161 tttgtcccct gaaattctga tacttgacga gcccacaaag gggcttgacg ccgcggcaaa
    17221 gagggatttg ggaatgctgc tgaaatccct taaatccgag ggaattacca ttatcaccgt
    17281 tacccatgac attgactttg ccgctgagtt tgcagaccgc tgcgctcttt tgtttgacgg
    17341 cgctgtggcc gctgagggcg ctgcgagaga gtttttcggg gaaaatttct tttacaccac
    17401 cgccgcatgc cgcatttcaa aaggctttat tgacggcgta ctcaccgtag aggaacttgc
    17461 gaataattta agacttaaag attagccgcg ctgacacaac aaaatatgcg taaggagaaa
    17521 gaattgaaaa agaagatacc catatacctt ttaatcttca tagccgctgc cctgcttgta
    17581 acgggagtgt tccgttttgc aaaggacaac tatgccctga ttacccttat aatggcggtt
    17641 ctttcaatgc tgcccctgtt tttgagcttt gagaaaaaga aagccgatac caaattattg
    17701 gctgtgcttg ccgctttaat tgcggtgtcg gtgctttccc gctttatctt tttcgccgtt
    17761 ccctttttta agcccgtaac ggctatgata attataatcg ccatgtattt gggctcggac
    17821 gcgggctttg cggcgggcgc cgcgacggct cttatctcta acttttattt cggtcagggg
    17881 ccttggaccg catttcagat gttctcatgg gggcttatcg gctttgccgc cgggcttctc
    17941 gccaaaaggc ttaaaaaaag caccgctttg cttcttgtct acggggcttt gtcgggggta
    18001 tttttctcat tgctgagcga cctttggtcg gttctctggt acgacggcgg ctttaatcta
    18061 aaaagatata ttgcccttgt aacctccgcg gcgggcatta ccctaaccta tgccgtatca
    18121 aatgtggttt ttcttctgat tatgaaaaat gtggtgggcg gcgccataga aagagtaaaa
    18181 cttaaatacg gaatcggcat tgactcttga ttattt
//
LOCUS       DAONVQ010000022        17624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2359_length_17624_cov_4.463202, whole genome shotgun sequence.
ACCESSION   DAONVQ010000022 DAONVQ010000000
VERSION     DAONVQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17624
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2359_length_17624_cov_4.463202"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLD98_02635"
     CDS             complement(<1..277)
                     /locus_tag="PLD98_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQA05179.1"
                     /translation="MLTIGASSACFYPDVTEESFIRLCKMNVKTAEIFFNSPSELEDS
                     FTSLLKSYAEEYGVSITAVHPFMSFAEHYNLFSNYERRFYDYMPLYLK"
     gene            373..669
                     /locus_tag="PLD98_02640"
     CDS             373..669
                     /locus_tag="PLD98_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05180.1"
                     /translation="MKELFNELKKIINSMNSFSKNVIKYGSIPVIAVYALAAIMYIFA
                     GRVVDYFFAMQICRDLLTMGKDFFSVIFVSALFFEIIATATGMKYVEAKNKDKD"
     gene            963..1799
                     /locus_tag="PLD98_02645"
     CDS             963..1799
                     /locus_tag="PLD98_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05181.1"
                     /translation="MSELKCSANPDVSSRRIKDAVCINTSKVYDSCADKDCLADLRVY
                     FTDCAQDVVDYATSVRVRKVDVLNVFTDIEKVPFNNGYYSIDLTFFFKVTLDVYTTPM
                     NPPTTIHGLAVYSKKIILFGGEGSVKVFSSEFTKKDFDEQLPAVETSPRVVVQVAEPV
                     ALDARICRPCDCCNNIADVQNGIPGCIKKCFPGQFVSPSAERAVRVTIGLFTIVQLER
                     DVQLLIPAYDFCIPTKDCTAVDEDACDAFRRIKFPIEEFFPPCDGDVAGESDDKPCGC
                     KK"
     gene            complement(2039..2551)
                     /locus_tag="PLD98_02650"
     CDS             complement(2039..2551)
                     /locus_tag="PLD98_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HQA05182.1"
                     /translation="MYSNKVMDMLKVQVNKEFYAAYLYLDMANFYADKNLDGFANWFY
                     VQAQEELDHAMLISKYLLNNGIRPELEAIAKPDKEFKELIDPLEAALEHEKYVTKCIH
                     EIYDAAMAEKDYRSKQLLDWFVLEQNEEEANADSLLQKMKMIGSGGLVFMLDAQLAQR
                     KHEPPSFDFD"
     gene            complement(2640..3446)
                     /locus_tag="PLD98_02655"
     CDS             complement(2640..3446)
                     /locus_tag="PLD98_02655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQA05183.1"
                     /translation="MKKFIVCLLKVLAVFTGWLALSHVADMYFEHTAVSRLFADLIVF
                     VGVVLVSYVFWLIDHKSFKVVNIEEPFKNIFKGILIGAVWVGVPLVILFITGKARIVS
                     VNHVDYFILWIISCFFSSVMQGLLIKGYIYQLIEHTYGFAAAVVVSTVLFTVMHHGVF
                     EAGPMTILNIASSSVAFSFMLRYSETIILPITAHFVWNLLGGMVLGAESMAHGYPALL
                     NIAFNESVLFDNAHKLEGSVLVLLINIVFILTLAILIHKKEHKTTAKAKV"
     gene            complement(3588..4772)
                     /gene="metK"
                     /locus_tag="PLD98_02660"
     CDS             complement(3588..4772)
                     /gene="metK"
                     /locus_tag="PLD98_02660"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQA05184.1"
                     /translation="MRKHTFTSESVTEGHPDKVCDQISDAILDAILEKDSRSRVACET
                     VTNTGLVLVTGEISTSSYVDIPAVVRSVLTDIGYNNPEYGIDGKHAAVITAIEEQSPD
                     IALGVDKAFEFKDGTMTDSEIEHLGAGDQGMMFGFACDETPELMPLPISLAHALSKRL
                     AEVRKKGIVPYLRPDGKTQVTVEYEDDRPVRVDTIVISTQHDPDATNAQIREDMIEHV
                     VKAVIPAHLLDEKTKYYINPTGRFVLGGPAVDSGLTGRKIIVDTYGGYAKHGGGAFSG
                     KDPTKVDRSGAYAARYIAKNIVAAGLARKCEVEIAYAIGMANPISVYVDTFGTGTEDE
                     QKIERAVIESIDLRPGAIIRDFDLRKPIYRNLAAYGHMGREELGLKFEERDLAAKILS
                     RL"
     regulatory      complement(4846..4945)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(5007..5996)
                     /gene="pfkA"
                     /locus_tag="PLD98_02665"
     CDS             complement(5007..5996)
                     /gene="pfkA"
                     /locus_tag="PLD98_02665"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HQA05185.1"
                     /translation="MKTIAVLTSGGDAPGMNAAIRAVVRTACENNMRVLGIRRGYNGL
                     MAGDMYEMNIRSVSDIIHRGGTILYSARSPRFNSEEGLKEAIEVCKANSIEGIVVIGG
                     DGSFRGARDLSIRGIPCVGVPGTIDNDIACTDYTIGYDTALNTIMDMVDRIRDTTESH
                     DRCSVIEVMGRHAGYLALNSGLAVGATSILLPEVDFNVERDVIERMRRTQKTGKKHFI
                     IMVAEGVAESMKTQGINGVGELAKLIEQRTGVETRATVLGHVQRGGSPSVKDRVVASQ
                     MGAAATLLLKEGKGNRVIAMRKEEIVDYDIYEALSMKKSIDLNLLKTALEISI"
     gene            complement(6239..7966)
                     /locus_tag="PLD98_02670"
     CDS             complement(6239..7966)
                     /locus_tag="PLD98_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05186.1"
                     /translation="MKKTFFRLYGFMRKSRGLLVLSVFSALTVVACNLLAPIALGRAV
                     DSMVGKGLVDFSSAYKYIALLASLYFGVCLFSWVLNRSANKIAYTTVGLIRKSMFDRL
                     LVLPSSFFDRTSYGDTASRFVNDADMVSDGLLQGLMALIQGVLTIVGASVIMLILNVP
                     MALTVMLSAPASFFVARYITLKSQKRFGQQAAIVGELNGYAEEYIDGLQLIKAFGRRE
                     KVLFEFGEINSRLYKAGLKAQIISALSNPTTRIVNNIAYAVIGVISGYLAINGLVTVG
                     TVSGFLIFAVIYARPFNEITSVLTQIQSAFASAERIFALLDETAEPSDENAPEHIEEC
                     RGEVEFKNVYFSYDPKKPLIEDLNLHIPQGSKVAIVGPTGAGKTTLVNLLMRFYDVDS
                     GSITVDGIDIRNLKRKNLREQFGMVLQDTWLFGGTVRQNIAYGKPDATFEEIVEAAKK
                     AGADSFIRRMEKGYDTRIRAGGENLSAGQRQLITIARIMLINPPMLILDEATSNIDTH
                     TELKIQRAFDTLTKGRTTFTIAHRLSTIINADTILVMDKGRIAEQGNHETLMKKNGLY
                     AALYNSQFS"
     gene            complement(7963..9690)
                     /locus_tag="PLD98_02675"
     CDS             complement(7963..9690)
                     /locus_tag="PLD98_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05187.1"
                     /translation="MRRLLVYLKPYRTDCIIGPVFKFLEVVFELLLPTIMASIINNGI
                     ENRDKQYVLKMGLLMAVMAILGYMSALICQILASRASQGFGTVLRNKVFGHIMSFSVS
                     DANNFGASTLTTRLSNDINQIQSFVAMTIRLSTRAPFICIGAVIMAFILNRRLALILA
                     ASIPVFAVIIFVITKLSAPLYKAFQMRLDDLSGTIRENIAGVRVIRAFSRNDAEKKHF
                     SKVNTDITETGFAIGRISAVFNPLTSLIVNTVVIIILWQGAISINAGTLSGGEIIAFI
                     NYTSQILSSLLIISNLIILSIKAFASAGRIAEILETAPEDEGSREIPELDMNAPAVSF
                     HNVSFVHNKSDELALKNINMTAFKGETIGIIGATGSGKSTFINMIGRFYKPSEGEIRI
                     FGVPIDEFPVSTLRKMIGIAPQRAVLFSGTVADNIRFGKPDATDEEVIKAAEDAQADS
                     FIREMPQGYDSRVSRGGKNLSGGQRQRISVARAIIGNPDILILDDATSALDFLTDSRL
                     RKAVKERRPDQTVFIVSQRVGVIKNADRILVFDDGEIVASGKHEELIRSSDIYKEICL
                     SQLSKEEIA"
     gene            complement(9860..10117)
                     /gene="spoIIID"
                     /locus_tag="PLD98_02680"
     CDS             complement(9860..10117)
                     /gene="spoIIID"
                     /locus_tag="PLD98_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HQA05188.1"
                     /translation="MKNIVEERAVELGEYIVENKATVRAAAKEFGISKSTVHMDVAER
                     LRKINPVLYQEVRSVLELNKAQRHIRGGLATKEKYRKLRDY"
     gene            10338..11867
                     /locus_tag="PLD98_02685"
     CDS             10338..11867
                     /locus_tag="PLD98_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HQA05189.1"
                     /translation="MKYEVKKDAVNAKELFYEGTLEQMIETEVNLPEYYCDVVKVLKC
                     TVCPVVASTQISGNRVVIEGNAKFSFMFLGEDNQVYSYESSYPFSKSTEIKDLDEQDS
                     VIAEARTDYVSCRASGKRQIAANATVSISIKVYRVKTLEIVTDCSIKGIQMKKKEISP
                     YSFIKVEQKGFTVSDTCEIQNFSGTVKRIVKSDATAYLTDVKTINGKMMISGEMQVKS
                     LAVTGGDEPDIIAVTSIIPFSQIFEISGVDENSICDVSVKVDHVDAQLQGSETSAAIV
                     YNIKATALICIGEVKNIQAVSDAYSTAKATETKKLQGNLKVIKARTTEPYMFNSQVDV
                     ADLEIGKIIDISVVGLKKNLRTDGSKVYVYGSVEIGMLYAYNDGRISYAEKSVSFEYE
                     KDTTLKAGAFECEANCECGGLSYSHASTDRLDIKAELKISLTVFEIESVDVLTDIEEK
                     GDSEFKAKPASLVIYFSDPGETVWDIARKYNTTVEKIAVENNLTDDVIRERRMLLIPC
                     V"
     gene            11923..13401
                     /gene="spoIVA"
                     /locus_tag="PLD98_02690"
     CDS             11923..13401
                     /gene="spoIVA"
                     /locus_tag="PLD98_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HQA05190.1"
                     /translation="MEDRNIYEDIATRTQGDIYIGVVGPVRTGKSTFIKKFMDTLVIP
                     NIEEGYQRERAIDELPQSAAGRTIMTTEPKFIPEEAVEIKLDNNAAFRVRMIDCVGYI
                     VPSSLGYIEGEQPRMVKTPWSDEELPFNVAAEMGTHKVIAEHSTIGLVVTTDGSISDI
                     PRYEYEEAEERVIKELQEINKPFIVLLNCMYPQSASARSLADELSEKYQVPVVPVNCI
                     ELSENEIVAVLTQVLYEFPVKEIEVELPAWLSSLNSDHWLKSSVFGQIKTSASKLTNM
                     RTVTYLSEEIAEMENISGANIESMSLGTGRAHIKVTMSNVLFYKVIEELTKIKIENEQ
                     DLMKTLTDLSMVKVKYERLKDALDEVEATGYGIVMPTIDELKLEEPEIMKQGGKYGVR
                     LKASAPSVHMLRADITTEVAPIVGSESQSEELVKYLLQGFEEDPQKIWESNIFGKSLH
                     ELVNEGLKNKLFHMPADARMKLQETLERIINEGCSGLICIIL"
     gene            13595..15916
                     /locus_tag="PLD98_02695"
     CDS             13595..15916
                     /locus_tag="PLD98_02695"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HQA05191.1"
                     /translation="MASFFVKLKSFFTALIFLFMSVFVPNITGPAQMPEVEMGEYARY
                     VDPFIGTGGLPWVSGMLFPGATAPFGLVRLSPDTAFPAGLNPFKMGTAGYYYGHNTIW
                     GFSHTRLSGTGAVDMGHFRVTPVTGKMSAADRLSSSLPFSHDKETATAGYYAVYLPSI
                     SCLAELTATQHVGVHRYSFDTSKDARILIDATSFLAGGRAEEGRVKVNPETYEVEGEA
                     RVFTGFTGRYGGLKGYFVAKFDTPFKSFATWEGSAVTEGKIEAFGNDTGADLNFGNLK
                     GKSVELKVGISFVSLENARENLEFETEGKNFDTVRDEAVAEWNAGLSKIKIDTNDKDI
                     KKIFYTALYHSMIMPTNFTDVNGQYLGFNEKVGVAEGYTYRTDLSLWDTVRTTHPLYT
                     LIEKEIQLDSVKSLIAMAEESGALPRWPSGAGESGSMFGTPADLLIAETYLKGLTDFD
                     AEKAYNFMKNTALETDDDSPAAKRDYNKEYLQYGYIPAQAGKRSVSYALEYAWEDYSI
                     ALLAEALGKFEDAEFFKERSKSYKNIFNPETKYFQAKSKNGTFVEPFSPYITTYYDEV
                     LPIKVSSAYVEGSPRQWRWSVQHDPQGLIELFGDRDYFISELEQFMKDATPKRAAIDP
                     GSGYWHGNQHDLHAVYLFIDAGRPDLAQKWIRWVLTDRYGTGADGLDGNDDGGTLSAW
                     YVLSAVGIYPMAGTDKYYIGAPIVDSAELDLGNGNILKVRAVNQSKDNIYVSEVTFNG
                     EKLTEPYITHALLDAGGELVFTMSPTPAENGGF"
     gene            complement(15962..>17624)
                     /locus_tag="PLD98_02700"
     CDS             complement(15962..>17624)
                     /locus_tag="PLD98_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HQA05192.1"
                     /translation="IALILLGKSLEAVSKGKTSEAVRKLMGLAPKTATVIHGGEEIEI
                     PIDEVMIGDIIAVKPGGKIPVDGTVVEGQTAIDESMLTGESMPVNKTVGDEVYAATIN
                     TTGSIKFRAEKVGSDTALAQIIKLVEDAQGTKAPIAKLADVVSGYFVPIVILIAVIAG
                     IGWFAAAKFGWADLPHGTSGLEFALTIFVSVLVIACPCALGLATPTAIMVGTGKGAEN
                     GILIKSGEALEIAHKIDTIVLDKTGTITEGKPSVTDIIAFEGFDNDTVLKAAASAEQG
                     SEHPLAQAIIDSAKEKGLPLSPALEFEALTGRGIRAKIEDAEILVGNKSLMEENNIGT
                     EAFSGFADNLSDEGKTPMFAAINGKPAGIIAVADTLKPTSKAAIKALHDLGLVTVMIT
                     GDNKKTAETIAKQVGIDKVLPEVLPKDKAEEVKKLQSEGRKVAMVGDGINDAPALAQA
                     DIGIAIGSGTDVAIESADIVLIRSDLKDVPTAINLSRRTIRNIKQNLFWAFAYNTIGI
                     PIAAGVLYIFGGPLLNPMFGAAAMSLSSVSVLTNALRLKRFKAKE"
BASE COUNT         4722 a         4393 c         4143 g         4366 t
ORIGIN      
        1 gctttaagta aaggggcata tagtcgtaaa atcttctctc gtaattgctg aaaaggttat
       61 aatgctcagc aaaggacata aaaggatgta cggcagtgat gctcacgccg tactcctcag
      121 cgtatgactt taaaagagag gtaaatgaat cttccagctc tgagggggaa ttgaaaaaaa
      181 tctctgccgt cttaacattc attttgcaga gccttataaa agactcctcg gtaacatcgg
      241 ggtaaaagca ggcagatgac gcgccaatag tcagcataaa tttcctttcg ccggcatata
      301 atgccttcag tttccataaa ttaaagcttg ttaatatatt ttattatagg tataatttat
      361 taggtgatac atatgaaaga gctcttcaac gagctgaaaa aaataataaa ttcaatgaat
      421 tccttttcaa aaaatgttat taagtacggc tcaataccgg taatagcagt atacgccctt
      481 gccgcaatta tgtacatatt cgcgggcagg gtagtggact atttctttgc tatgcagatt
      541 tgcagagacc ttctgaccat gggcaaagac tttttcagcg tgatattcgt atccgccctt
      601 ttctttgaga ttattgccac ggctacggga atgaaatatg tcgaggcaaa aaacaaggat
      661 aaagactgac cgcttactcc tatttaatta tacattacca tccgtaaaac aggggtcccg
      721 ggcattagcc cggggctttc tgttttttct tttttcgcac cgtcatttta tcaccaatac
      781 actttaaagt caatttttta tgtcggaact aacgctttcg caaaaatgaa agaataaaac
      841 ttttaaataa atctttgagc caataagttg aaagcaaatt taagtaacca aaattcgccc
      901 tcctcataaa ctaatattga agtttacgaa atttcaatta ttaattaata ggaggaaaat
      961 gtatgtctga attaaaatgc agcgcaaatc ccgatgtaag ttcaaggcgt ataaaagatg
     1021 ctgtatgcat taacacaagc aaggtatacg attcatgtgc tgataaggat tgtctggccg
     1081 atctcagagt atactttacc gattgtgctc aggatgttgt agactacgca accagcgtaa
     1141 gagtccgcaa ggtagatgtt ctgaatgtct ttaccgatat tgaaaaggtt cccttcaaca
     1201 acggatacta ttcaattgac ctgacattct tcttcaaggt aacccttgat gtctacacca
     1261 ccccgatgaa tccgcccaca acgattcacg gtcttgcagt atacagcaag aagatcatcc
     1321 tcttcggcgg cgaaggcagc gtaaaggtat tctcatctga gttcaccaag aaggatttcg
     1381 acgagcagct gcccgcagta gagaccagcc cgagagttgt ggtacaggtc gcggagcccg
     1441 tagcgcttga cgcaagaatc tgccgtccct gcgactgctg caacaacatc gccgatgtgc
     1501 aaaacggcat acctggctgc ataaagaagt gcttccccgg ccagtttgta agcccgagcg
     1561 ccgaaagagc agtaagagtc accatcggtc tgttcacaat cgttcagctt gagagagatg
     1621 ttcagctcct tatcccggca tatgacttct gcataccgac caaagactgc acagccgttg
     1681 acgaagacgc ctgcgacgca ttccgccgca taaaattccc cattgaggag ttcttcccgc
     1741 cctgcgacgg agatgtagca ggagagagcg atgacaaacc ctgcggctgc aagaaataaa
     1801 gtacctcaag tcaaacagca ttagccttaa caaaatcaaa gtcagcctac aaagtatgaa
     1861 gccaaggaaa ccccttggct tcactttttt tgaagggctc aaagcccccg cctaaagttt
     1921 tagtccgaat agagtgaccc gccaaacgcc tttcgaaatt aaacccgccg caccgccccc
     1981 cgtcagaata aatacaaaaa ccctgccctt ttgggcaggg tcttttatgt aagctcaatt
     2041 aatcaaagtc gaatgaaggg ggctcatgct ttctctgagc aagctgtgcg tcaagcatga
     2101 ataccagtcc gccggagcct atcatcttca tcttctgcag cagggaatcg gcgtttgcct
     2161 cctcctcatt ctgctccaga acgaaccagt caagcagctg cttggagcgg taatccttct
     2221 ctgccattgc cgcatcatag atttcgtgga tgcactttgt tacatacttt tcatgctcca
     2281 gagcagcctc aaggggatcg atgagctctt tgaattcctt atcgggctta gcgatagcct
     2341 ccagctcagg cctaatgccg ttattcagca gatattttga aatgagcatt gcgtggtcaa
     2401 gctcttcctg tgcctgaaca tagaaccagt ttgcaaagcc gtccaagttt ttgtcagcgt
     2461 agaaattcgc catgtcaaga tagagatatg cggcataaaa ctccttgttg acttggactt
     2521 taagcatatc cattacttta ttgctataca tacaaacatc cccttttctt tctgtttttt
     2581 acattatata tcaatttatt ctaaaccaca aggaaactgc ggataaattt tccaaaagtt
     2641 taaacttttg ccttagcggt ggtcttatgc tcctttttat gaatcaaaat tgcgagagtc
     2701 agaatgaaaa caatgtttat aaggagcacc agaacgctgc cttcgagttt atgggcgttg
     2761 tcaaagagca cgctttcatt aaaggctata ttcaaaagag cggggtagcc gtgagccatg
     2821 gactcggctc ccaaaaccat tccgccgagc agattccata caaaatgtgc ggtgatgggc
     2881 aggataatcg tctccgagta cctgagcata aatgagaacg ccacgcttga cgacgcgatg
     2941 ttaagtatag tcatcgggcc cgcctcaaaa acaccgtggt gcatgacggt aaagagtacg
     3001 gtggaaacga cgaccgcggc cgcaaagccg taagtgtgct caatgagctg atagatatag
     3061 ccctttatta aaagtccctg cattacggag ctgaagaagc aggaaataat ccagaggata
     3121 aagtaatcca catggtttac gctgactatt ctcgccttgc ccgtaataaa cagtatcaca
     3181 aggggtacgc cgacccatac ggcgccgatt agaattccct tgaaaatatt cttaaagggc
     3241 tcttcaatat tgactacctt aaagctctta tggtcaataa gccaaaatac ataggatacg
     3301 agaacaactc ccacaaaaac tataaggtcg gcaaaaagcc ttgataccgc ggtgtgctca
     3361 aaatacatat cggctacatg ggaaagcgcg agccagcctg taaaaactgc caataccttc
     3421 aaaagacaaa caatgaactt tttcacacta tctctctttt ctttgcccaa agtcttttaa
     3481 tattatatac actaaaagac tcttatgtaa atatatagta acttctcttt aagttctgtg
     3541 acctgggtta caaaaaaaga ggcttacgca gcctcttttt cttttcttta gagacggctg
     3601 agtatctttg ccgccaaatc tctttcttca aatttcagtc ccagctcctc gcgtcccatg
     3661 tgaccgtatg cggcgaggtt tctgtagatg ggctttctta aatcaaagtc cctaataatg
     3721 gcgccgggtc ttaaatcaat gctttcaatt acagcccgct cgattttctg ctcatcctca
     3781 gtgcctgtgc cgaaggtgtc aacataaacc gaaatcgggt tcgccatgcc gatggcgtat
     3841 gcgatttcaa cctcgcactt tcttgcgaga cccgccgcga cgatgttctt tgcgatatac
     3901 cttgcggcgt atgcgcccga gcggtcaacc tttgtcgggt cttttcccga gaaggctccg
     3961 ccgccgtgct tagcgtagcc gccgtatgtg tcaacgatga ttttccttcc cgtaagaccc
     4021 gagtcaacag cggggccgcc cagaacaaag cgtcccgtgg ggttaatata gtactttgtc
     4081 ttttcgtcaa ggagatgagc ggggataacc gcctttacaa catgctctat catatcctct
     4141 cttatctgag cgtttgttgc gtcggggtcg tgctgagtgg atatgactat tgtgtcaact
     4201 ctaacggggc ggtcgtcttc gtattcaacg gttacctgag tcttgccgtc gggtctgaga
     4261 tagggtacaa tgcccttctt tctgacctca gccagcctct ttgaaagggc gtgggcaagg
     4321 gatatgggca gaggcataag ctccggggtt tcatcgcagg caaagccgaa catcatgccc
     4381 tggtcgcctg cgcccagatg ctctatttcc gaatcggtca ttgtgccgtc cttaaactca
     4441 aaagctttgt caacgcccag agctatgtcg ggagactgct cctcaatggc agtaataaca
     4501 gcggcatgct tgccgtcaat gccgtattcg ggattgttgt agcctatgtc tgtcagaacg
     4561 gagcggacaa cagcggggat gtccacatag cttgaggtcg aaatctctcc cgttaccaga
     4621 acaaggcctg tatttgtaac cgtctcacag gcaaccctgc tccttgaatc cttttcaagg
     4681 atagcgtcaa gtattgcgtc ggaaatctgg tcgcaaacct tgtcgggatg tccctctgtt
     4741 acggattcgg atgtaaaagt gtgctttctc attttaggtt tcctttcgtt tttgtcatgt
     4801 ctcataaaca aatacccggc atagcaactg ccgggatgac tttaaatctc atcttgcggc
     4861 aaatccgcag gaattggcac cttgtaatcg cttacaggtt gccgggtttc acagggcctt
     4921 ttccctccac cactcttgat aagatgtatt aaatattcac ttttattatt atacgaaaag
     4981 cctcagatta tgtcaagaaa taaaggttaa atggagattt caagggcggt tttgagaaga
     5041 tttaagtcaa tgctcttctt cattgaaaga gcctcgtaga tatcgtagtc aacaatctcc
     5101 tctttgcgca tggcgatgac tctgttgccc ttgccttctt taagcagaag tgttgcggca
     5161 gcgcccatct ggcttgccac gactctgtcc ttaacggagg gtgagccgcc tctctgaaca
     5221 tgaccgagaa cggtagccct tgtctcaacg cctgttctct gctcaatgag cttcgcaagc
     5281 tcgccgacgc cgttaatgcc ctgagtcttc atggattcgg caacgccctc tgccaccatt
     5341 atgataaagt gctttttgcc ggtcttctga gtgcggcgca ttctttcaat gacatcgcgc
     5401 tccacattaa agtcaacctc gggcagcaga atggatgtag ctccaacggc aagaccgctg
     5461 ttaagggcga gatagcctgc gtgtctgccc ataacctcaa ttacggagca gcggtcgtgg
     5521 gactctgtgg tgtccctgat tctgtccacc atatccatta ttgtattaag agccgtatca
     5581 tagccgattg tgtaatcggt gcatgctatg tcgttgtcaa tggttccggg aacgcctaca
     5641 caggggattc ctctgatgga aaggtcgcgg gcgcctctga aggagccgtc gccgccgata
     5701 acaacaatgc cctcaatgga gttagccttg cagacttcaa ttgcctcttt aagaccttcc
     5761 tcggagttga atctcgggct tcttgcggaa taaagaatgg ttccgcccct gtgaataatg
     5821 tccgaaacgc tccttatgtt catctcgtac atatcgcctg ccataagacc gttatagccc
     5881 ctgcgaatgc cgagaactct catgttgttt tcacatgccg tgcgtactac agcgcggata
     5941 gccgcattca tgccgggcgc gtcgccgccg cttgtcaaaa ctgcaatggt tttcattgtt
     6001 gttcctccaa aacttataaa gcctctcgtc ttaaagcgag ccgaacgaca agctaaaagt
     6061 gagggtgttt ttgcggttta atatttatgc gaaaaaacaa ttccaactat aaatattaaa
     6121 ctcataaaga tacaaaagtc aatagaaaaa agctcctatt ggcttctttt gtcgcttagt
     6181 acagactgtc cgatgaatta aagaaaaaat tgttgaaaga ttaacaaaat ccatgagttt
     6241 aagagaactg actgttatac aaagcggcgt aaagaccgtt ctttttcatc agagtttcat
     6301 ggttgccctg ctccgcaatc ctgcccttgt ccataacgag aatggtatcc gcgtttataa
     6361 ttgtggagag cctgtgggca attgtgaagg tggttctgcc ctttgtgagg gtgtcaaacg
     6421 ccctttggat tttcagttcc gtatgggtgt ctatattgct tgtagcctcg tcaagtataa
     6481 gcatgggcgg gtttattagc attatccttg ctattgtgat aagctgcctt tgacccgccg
     6541 acagattttc accgcccgct cttatacggg tgtcgtagcc cttttccatt cttcttataa
     6601 agctgtcggc gccggctttt ttcgccgcct caactatttc ctcaaatgtt gcgtcgggct
     6661 ttccgtaggc aatgttctgc cttacggtgc cgccgaaaag ccatgtgtcc tgcagtacca
     6721 ttccgaactg ctctctgagg tttttcctct ttaagtttct tatgtcaatg ccgtcaacgg
     6781 tgatggagcc cgagtcaaca tcataaaaac gcataaggag atttacaagc gttgtcttgc
     6841 ccgcgcccgt gggacccaca atggcgactt tgctgccttg ggggatgtgc aaattcaaat
     6901 cctcaatcaa gggctttttc gggtcatagg aaaagtatac atttttaaac tccacctctc
     6961 cgcggcactc ctcaatatgt tcgggagcgt tctcatcgga gggctcagcc gtttcgtcaa
     7021 gcaaagcgaa tatcctctca gccgacgcaa aggcggactg aatctgcgtc agaaccgagg
     7081 taatctcatt aaagggtctt gcgtagataa cggcgaaaat caaaaagccg gaaaccgtgc
     7141 ccacggtgac aagcccgttt attgcaagat agccgcttat aacgcctatc accgcatagg
     7201 caatattgtt tactatcctt gttgtggggt ttgagagagc ggagataatc tgagccttca
     7261 ggcccgcctt ataaagcctt gagtttattt ctccgaactc aaagagaacc ttttcccgcc
     7321 tgccgaacgc ctttataagc tgcaatccgt caatatactc ctcagcgtag ccgttaagct
     7381 cacccacgat agccgcctgc tgaccgaacc tcttttggga tttaagggtg atatacctcg
     7441 ccacaaagaa ggaggctggt gcggagagca tgacggtcag cgccatgggg acattgagaa
     7501 tcagcattat taccgacgcg cccacaattg tcagtacgcc ctgaataaga gccataagac
     7561 cctgcaatag tccgtctgat accatgtccg cgtcgttcac aaagcggctg gcggtatcgc
     7621 cgtaggatgt cctgtcaaaa aacgaggacg gcagcaccag cagcctgtca aacatacttt
     7681 ttcttattag tcccactgtg gtatatgcga ttttgttagc cgagcgattg agtacccagc
     7741 tgaaaaggca gacgccgaaa tataaagagg cgagaagggc tatatatttg taagccgagg
     7801 agaaatccac aagccccttg cccaccatgc tgtccacagc cctgccgagg gctatggggg
     7861 caaggagatt acaggcaacc acagtcagcg cgctgaatac agagagaacc aaaagccccc
     7921 tgctctttct cataaagccg tacagcctaa agaaggtttt tttcatgcta tctcctcctt
     7981 tgaaagctgc gacaggcaaa tctctttgta tatgtcgctt gagcggataa gctcctcatg
     8041 cttgccgctg gcgacgattt cgccgtcgtc aaatacaagt atgcggtcgg cgttctttat
     8101 tacgcccaca cgctgggaga ctataaaaac ggtttggtcg ggtcgacgct ctttaaccgc
     8161 cttcctgagc cttgagtcgg ttaaaaagtc aagggcgctg gtggcgtcgt caagtatcag
     8221 aatatcgggg tttccgataa tagcccttgc tacggaaatc ctctgccttt gaccgcccga
     8281 gaggtttttg ccgcccctcg acactctcga atcataaccc tggggcatct ccctgataaa
     8341 tgagtctgcc tgagcgtctt cggcagcctt tattacctcc tcatcggtgg cgtcgggctt
     8401 tccgaaacgg atattgtccg ccacagtgcc ggagaaaagc acagcccttt gcggcgcgat
     8461 gcctatcatt tttcttaagg ttgatacggg gaattcgtca atcggaacgc cgaaaatcct
     8521 gatttcccct tccgagggct tataaaacct tcctatcata tttataaatg tggactttcc
     8581 cgagcctgtc gcccctatta ttcctatggt ctcgccctta aaggctgtca tgtttatatt
     8641 ctttaaagca agctcatccg acttattgtg aacaaaggac acattatgaa atgatacggc
     8701 gggggcattc atgtcaagtt cgggaatctc ccgagagccc tcatcttcgg gagcggtttc
     8761 aagtatctcc gcaatcctgc ccgccgacgc aaaggctttg attgagagaa taataaggtt
     8821 tgatataata agcagcgagg acaaaatctg gcttgtgtaa tttataaagg caataatctc
     8881 tccgcccgac agagtgcctg cattaatgct tatagctccc tgccagagaa tgataatgac
     8941 cacggtattt acgataagtg aggtcagggg attgaaaacg gcggaaatcc tgcctattgc
     9001 aaaacccgtc tccgttatat ccgtatttac ctttgagaaa tgttttttct ccgcgtcgtt
     9061 tcttgaaaac gccctgatga ctctgacgcc cgctatgttc tctcttattg ttccgctcaa
     9121 atcgtcgagc ctcatttgaa aagccttgta aaggggagcg gacagctttg taattacaaa
     9181 gataataact gcaaaaacgg gaatggaagc ggcgagaatt aaagcaagcc tcctgttaag
     9241 aataaacgcc ataataaccg cgcctatgca tataaacggc gcgcgggtgg aaagccttat
     9301 ggtcattgcc acaaagcttt gaatctggtt tatgtcgttg gaaagtctgg tggtaagggt
     9361 ggaggctccg aaattgttgg cgtcggagac cgaaaaggac attatatgcc cgaaaacctt
     9421 gtttctgagc actgtgccaa atccctgaga cgctctggag gcaagaatct ggcatataag
     9481 agcggacata tagccaagaa tcgccatgac cgccattaaa agccccattt tcagcacata
     9541 ctgcttgtcc ctgttttcga tgccgttgtt tattatagat gccataatgg taggcagcag
     9601 cagctcgaaa accacctcaa ggaatttaaa aacgggacct attatgcagt ccgttctgta
     9661 tggttttaaa tagaccaaaa gacgccgcaa aaaatcagct cccaaagcat aataatattc
     9721 ctattataca tattttaccc atcaatgtaa attaaagcgg aatatcaaaa gaaaaagcat
     9781 cggctcccca taagggagcc gatacttttg agagtgaaaa aacttaattg ggagcaaata
     9841 aattcagcgg gaaacagaat taataatccc ttagctttct gtatttctcc tttgtggcaa
     9901 gaccgcctct gatatggcgc tgtgccttgt taagctccag cacgcttctt acttcttgat
     9961 aaagaacggg attaattttt cttagccgct ccgcaacatc catatgtacc gtggacttgc
    10021 ttattccgaa ttccttagcg gctgctctca cagtagcttt attttcaact atatattcac
    10081 caagctctac tgctctttcc tcaactatat tcttcaataa aaaccctccc gcttaacata
    10141 tcgttacata gatatgccag acaaatttga tatatgatac gggacaaatt catataattt
    10201 aaaaaaacgc ttttaaagga caaaaaagta aaaaagtgcc caaaaacata aagacaagat
    10261 gcaaatgctt ggcatactcg ggacaggatg tgaatataga ttgcatagag actttttaat
    10321 aaaggagaca gccagatatg aagtatgagg taaaaaagga tgccgttaat gccaaagagc
    10381 ttttttacga gggtaccctt gaacaaatga tagaaaccga agtaaacctt cctgagtatt
    10441 actgcgatgt tgtcaaggta ctcaagtgca cggtatgccc ggttgttgcc tcaacccaga
    10501 tttcgggcaa cagagtggtc atagagggca acgcgaagtt cagctttatg ttcctcggag
    10561 aagacaacca ggtgtactct tacgaaagct cttatccctt ctcgaaaagc acagagatta
    10621 aagaccttga cgagcaggat tcggtcattg ccgaggcaag aaccgactat gtaagctgca
    10681 gagcctccgg caagaggcag attgcggcca acgccacggt ttcaatctca atcaaggttt
    10741 acagggtaaa gacccttgag attgtcaccg actgctcgat taaaggcatt cagatgaaaa
    10801 agaaggaaat aagcccctat tcatttataa aggtcgagca gaagggcttt acggtaagcg
    10861 acacctgcga gattcagaac ttcagcggta ccgtaaagag gattgtaaag agcgacgcca
    10921 ccgcgtatct gaccgatgtc aagaccataa acggcaagat gatgataagc ggcgaaatgc
    10981 aggtcaaatc ccttgccgta acgggcggcg atgagccgga catcatagcc gtaacaagca
    11041 taatcccctt cagtcagata ttcgaaatca gcggcgtcga cgaaaactca atctgcgatg
    11101 tttctgtaaa ggttgaccat gtggacgccc agctccaggg cagcgaaacc tcggccgcca
    11161 tagtctacaa cataaaggca accgccctta tctgcatagg cgaggtcaag aacattcagg
    11221 cggtcagcga cgcctactca acggcaaagg ctacggagac aaagaagctg cagggcaatc
    11281 ttaaagtcat aaaggcaaga accacagagc cctatatgtt caattcacag gttgatgtag
    11341 ccgaccttga gataggaaag ataatcgaca taagcgtcgt gggacttaaa aagaacctca
    11401 gaaccgacgg ctctaaggtt tatgtctacg gcagcgtcga aatcggtatg ctctacgcct
    11461 acaacgacgg cagaataagc tacgccgaaa agagcgtcag cttcgaatac gagaaggata
    11521 caactcttaa agcaggagcg tttgagtgcg aggcaaactg cgagtgcggc ggactcagct
    11581 acagccacgc ctccacagac aggctggata ttaaggcgga gcttaaaata tcccttaccg
    11641 tattcgaaat cgaaagcgtc gatgtactga ccgatattga ggaaaaaggc gacagcgaat
    11701 ttaaggcaaa gcccgcaagc cttgtaatct acttctccga cccgggcgaa accgtttggg
    11761 acatagccag aaagtacaac accacggtgg agaaaatcgc tgtcgaaaac aaccttaccg
    11821 acgatgtgat aagggaaaga cgaatgctcc ttatcccttg cgtttaacat agatttaatt
    11881 taaagccatc tgcataccga gaatgcgaag gagaggatat aattggaaga ccgcaacata
    11941 tacgaagata ttgctacacg cacacaaggg gatatataca taggtgtggt cggacccgtc
    12001 agaacgggta aatccacctt cataaagaag ttcatggaca cacttgttat ccctaatatt
    12061 gaagagggtt atcagagaga gcgggcaatc gacgagctgc cccagtccgc cgccggcaga
    12121 acaattatga ccacggagcc caagttcatc cccgaggagg cagtcgagat taaactcgac
    12181 aacaacgccg ccttcagagt acggatgatt gactgcgtcg gctatatagt tccgagttct
    12241 ctcggctaca tcgagggcga gcagcccaga atggtaaaga ccccgtggag cgacgaggaa
    12301 ctgcccttca atgtggcagc cgagatgggc acccataagg tcatagccga gcactccacc
    12361 ataggtcttg tggtcaccac cgacggcagc atcagcgaca ttccccgcta cgagtacgag
    12421 gaggcggagg aaagggtaat caaggagctt caggagatta acaaaccctt cattgtgctc
    12481 cttaactgca tgtatcccca gtcagcctcc gcaaggagcc ttgccgacga gctctccgaa
    12541 aagtatcagg tgcccgtagt gcccgtaaac tgcattgagc tgtcggagaa cgaaatcgtg
    12601 gcggtcctga ctcaggttct ctatgagttc cccgtaaagg aaatcgaagt ggagctgccc
    12661 gcatggctct cgtccctcaa ctccgaccac tggctcaaga gctcggtatt cggtcagatt
    12721 aagaccagcg cttctaagct caccaatatg cgcacggtca catatctgag cgaagaaatc
    12781 gccgagatgg agaacatcag cggcgccaat atcgagtcca tgagcctcgg caccggcaga
    12841 gcccacatca aggtcacaat gagcaatgtc ctgttctaca aggtcatcga ggagctgact
    12901 aagattaaga ttgagaacga gcaggacctc atgaagacgc ttacggacct gtcgatggta
    12961 aaggtcaaat acgagcggct taaagacgcc cttgacgagg ttgaggctac gggctacggc
    13021 atagtaatgc ccaccataga tgagcttaaa ctcgaggagc ccgagattat gaagcagggc
    13081 ggcaagtacg gagtaaggct caaggcatcc gcgccgtcgg tacacatgct cagagccgac
    13141 attacaaccg aggtcgcccc gatagtgggc tccgagagcc agtccgagga gcttgtaaaa
    13201 tacctgctcc aaggctttga ggaggacccg cagaagattt gggagtccaa catcttcggc
    13261 aagtcgctgc atgagcttgt aaacgagggt ctgaagaata agctcttcca catgcccgcg
    13321 gacgccagaa tgaagctgca ggaaacactg gagagaatca tcaacgaggg ctgcagcgga
    13381 ctgatttgca taatccttta acctcatttc attgccgcct cgaatttatt tcgggcggcg
    13441 tcggaacgaa acaaccggct aaacacttag ccggtttttt tcttttctga gaattagatt
    13501 tggagaaatt acatattaat gcttgttttt tccatgcgga aactgtatga tttaaataga
    13561 caagccgcag tttacataaa acggaggttg taaaatggca tcgttctttg ttaaactcaa
    13621 gagctttttt acggcgctta ttttcttgtt tatgtccgtt tttgtgccaa acataacggg
    13681 gcccgctcaa atgcccgaag tggaaatggg cgagtacgcc cgttatgtcg acccatttat
    13741 cggtacgggc ggtcttccgt gggtaagcgg aatgctcttc cccggagcca cagcgccatt
    13801 cggacttgtg cgcctcagcc ccgacaccgc gttccccgca gggctcaacc cctttaagat
    13861 gggcacagcg ggctactatt acggtcataa cacaatctgg ggcttcagcc acacaaggct
    13921 ttcgggtacg ggagccgttg acatgggcca cttcagagta acccccgtta cgggcaaaat
    13981 gagcgcggcc gacaggcttt caagctcact gcccttcagc cacgataagg aaaccgccac
    14041 ggcaggatat tacgcggttt atctcccctc aatcagctgc cttgcggagc ttaccgcaac
    14101 ccagcatgtg ggagttcaca gatacagctt tgacacaagc aaagacgccc gaatcctcat
    14161 cgacgccacc agcttccttg caggcggcag agcagaggag ggcagagtaa aagtaaatcc
    14221 cgaaacctat gaggtagagg gcgaggcaag agtctttaca ggctttaccg gcaggtacgg
    14281 cggtcttaaa ggctactttg tcgcaaaatt cgacacgccc tttaaatcct tcgccacatg
    14341 ggaaggttct gccgtaacag aaggcaaaat cgaagccttc ggaaacgaca cgggcgccga
    14401 ccttaatttc ggcaacctta aaggcaagtc ggttgagctg aaagtcggaa taagctttgt
    14461 aagcttagag aacgcaaggg aaaaccttga attcgaaacc gaaggcaaaa actttgatac
    14521 ggtaagagac gaggctgttg cagagtggaa cgccggactc tcaaaaatca aaattgatac
    14581 aaacgacaag gatataaaga aaatctttta caccgccctt tatcactcaa tgataatgcc
    14641 caccaacttt accgatgtta acggtcagta tctgggtttt aacgaaaagg tgggagtcgc
    14701 cgagggctat acctacagaa ccgacctttc cctttgggat acggtcagaa ccacccaccc
    14761 gctgtatact cttatcgaaa aggagattca gcttgactcg gtaaagagcc ttatcgccat
    14821 ggcggaggag tcgggcgcgc tgcccagatg gccctccggc gcgggagaga gcggctctat
    14881 gttcggcacc ccggcggacc ttctgattgc cgaaacctac ctcaagggtc taaccgattt
    14941 tgacgcagaa aaggcgtata actttatgaa gaataccgcc ctcgaaaccg acgacgattc
    15001 ccccgccgca aagagggatt acaacaagga gtacctgcag tacggctata tccccgccca
    15061 ggcgggcaag cgctccgtaa gctacgctct cgaatacgct tgggaggatt acagcatagc
    15121 ccttcttgcg gaggctctgg gcaagtttga ggacgcggag ttcttcaaag agcgctcaaa
    15181 gtcgtataaa aacatcttca accccgagac aaaatacttc caggcaaaga gtaaaaacgg
    15241 cacttttgtt gagcccttca gtccctacat caccacctac tatgacgagg ttctgcctat
    15301 aaaagtatca agcgcctatg ttgagggcag cccgagacag tggagatgga gcgttcagca
    15361 cgacccgcag ggtcttatag agctctttgg cgacagggat tactttatct ccgagcttga
    15421 gcagtttatg aaggacgcga cgcccaaaag ggcagcaatt gaccccggtt cgggctactg
    15481 gcacggcaac cagcatgatt tgcatgctgt ttacctcttt attgacgcgg gcagacccga
    15541 ccttgcccag aagtggataa gatgggtcct gaccgaccgc tacggaacgg gagccgacgg
    15601 acttgacggc aacgacgacg gcggtacctt aagcgcatgg tatgtcctct ccgcagtggg
    15661 catttacccg atggcgggaa ccgacaaata ttatatcggc gcgcccattg tggacagcgc
    15721 ggagctcgat ttgggtaacg gaaatatact caaagtcagg gctgtcaatc agtcaaagga
    15781 caatatttat gtaagcgaag taaccttcaa cggcgaaaag ctgacagagc cttatatcac
    15841 ccacgctttg ctcgacgcgg gcggagaact ggtattcacc atgagcccga cccccgctga
    15901 gaacggcggc ttctaaagaa aaataaaaag ggcaggctaa aagcctgccc atctttttta
    15961 tttactcttt agctttaaac cttttaagtc tcaaggcgtt tgtcagcacc gaaacggagc
    16021 tgagactcat tgccgccgcg ccgaacatgg ggtttaggag cggcccgccg aagatataca
    16081 gaacgcccgc ggcaattggt atgcctatag tattgtaagc aaacgcccag aaaagattct
    16141 gcttaatgtt cctaatcgtc cgcctgctga gatttatagc tgtggggaca tcttttaggt
    16201 cggagcggat aagaactatg tccgccgatt ctatcgccac atccgtgccc gagccgatgg
    16261 ctatgcctat atccgcctga gcgagagcgg gagcgtcgtt tatgccgtcg cccaccatgg
    16321 cgacctttct gccttcagat tgcaactttt tcacttcttc cgccttgtcc ttgggcagca
    16381 cctcgggcag aaccttgtca atgcccacct gttttgcgat tgtctcggcg gttttcttgt
    16441 tgtcgcccgt aatcattacc gtcacaagac ctaaatcgtg cagcgctttt atcgccgcct
    16501 tgctcgtggg cttgagagtg tccgcaaccg ctattattcc tgcgggcttg ccgtttatcg
    16561 ctgcaaacat cggcgtcttt ccctcatcgg agagattatc cgcaaagcct gaaaatgcct
    16621 cagtgcctat attgttttct tccatcagag acttgttgcc cacaaggatt tcggcgtctt
    16681 ctattttagc cctgatgccc cttcccgtca gagcctcgaa ttcaagggcg ggggagagcg
    16741 gcaagccctt ttccttcgcg ctgtcaataa tagcctgtgc caaggggtgc tccgaaccct
    16801 gctcagccga cgctgcggcc ttcagaacgg tatcgttgtc aaaaccttca aaggcgataa
    16861 tatccgtaac cgacggtttt ccttccgtga ttgtgccggt cttgtcaagg actatggtgt
    16921 caatcttgtg ggcaatctcc agagcctcgc cgcttttaat tagaattccg ttctccgcgc
    16981 ccttgccggt gcccaccata atcgccgtgg gagtggcaag gcccaaagcg catgggcagg
    17041 cgattacaag gacggaaaca aaaatcgtaa gggcgaattc aagccccgat gtgccgtgtg
    17101 gaaggtcagc ccagccgaat tttgccgcgg cgaaccagcc gatgcccgca atcaccgcaa
    17161 taagaatcac aatcggaaca aaatatcccg aaaccacatc ggcgagcttc gcaatcggag
    17221 ccttagtacc ctgagcgtcc tcaaccagtt ttataatctg ggcaagagca gtgtcggagc
    17281 caaccttctc agccctgaac tttatgctgc ccgttgtgtt aatcgtggcg gcataaacct
    17341 catcgccgac tgtcttgttt actggcatgc tctcacccgt cagcatcgac tcgtcaatgg
    17401 cggtctgtcc ttcaacaaca gtaccgtcaa cgggaatctt gccgccgggc tttactgcaa
    17461 taatgtcgcc gatcataacc tcgtcaatgg ggatttcgat ttcttcgccc ccatgtataa
    17521 cagtcgccgt ctttggggca agacccatca gcttccttac agcctcgctt gtcttgccct
    17581 ttgaaaccgc ctcaagggac ttgccgagga gtatcagagc aatg
//
LOCUS       DAONVQ010000023        17548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2377_length_17548_cov_4.765621, whole genome shotgun sequence.
ACCESSION   DAONVQ010000023 DAONVQ010000000
VERSION     DAONVQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 17548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17548
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2377_length_17548_cov_4.765621"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..2178)
                     /locus_tag="PLD98_02705"
     CDS             complement(73..2178)
                     /locus_tag="PLD98_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQA05193.1"
                     /translation="MLSSKKTNLKRVTSALIAIFSILGIFVVQLFRIQLINRDEIAAS
                     KNVDVYTLKVEGARGEILDRNGNVLATNRQGNRIVFSAAHFPSLKNQSERNEIIISLI
                     KLLESKGEEWNDNLPLILNPDGSVSFKENSDSYIEYLKSPDMLNLNHYATAQNCFDAL
                     KEYFSLQDYSDEDAIKIASVCVSLRKQGFSITNPYVFAEDVSSATIAAIKERSDFYRG
                     VEHEVVTYREYPEKSLAAHLIGTVGAISQSEYENAKNALEQKLKDPNLTEEEKRALEK
                     RSYDLDDIYGKSGVEKLMEEYLKGADGYKVISRDTDGNLKESYIEEPEKGETVILTID
                     AGLQKAAEIALEKRIKELTAQKGLEAAGAVAVIDVNTGELLASASYPGYDLSTYYENY
                     SKLASDPAAPLWNRVLQSTYAPGSTFKPLVAIAALETGTVNKYDQYICNSVFHYKGMD
                     FRCLKSHGRTNVVEAIEKSCNIYFYNVADILGINKMNEYATAFGLGSKTGIELPEAEG
                     VLAGIPYRESLGKLWLPGDTIQASIGQSDNLFTPLQLVNYCAAIANGGTRYVPHIIKE
                     VKTPDKSSTIMKKDPAVAFKTNISASTFATVKEGMLRAVTQGSLQSTFKDLKVQAAAK
                     TGTSQTVKIVNGRAVKGNNGFVISFAPYENPEIAVVVLIENVDSGTATAKVAADIYEY
                     YFGTMKSVVPQQPFNTLLP"
     gene            complement(2199..2705)
                     /locus_tag="PLD98_02710"
     CDS             complement(2199..2705)
                     /locus_tag="PLD98_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05194.1"
                     /translation="MPFKNNRQMHIYRAILAVLVIAVSVLHTTPSVPILSSFYAFIPL
                     VICLGLFERELSGALYGALAGIIYDISSPLPDGFNAIFFCLMGFACGLLVRYILRNNL
                     VSALILTVFFGGLYTLFRCIFLRKIYGEALKLFSPLALTALFLPVFYLLIRYISKHFK
                     TSDDLDKI"
     gene            complement(2727..3575)
                     /gene="mreC"
                     /locus_tag="PLD98_02715"
     CDS             complement(2727..3575)
                     /gene="mreC"
                     /locus_tag="PLD98_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQA05195.1"
                     /translation="MKTYIRTTAFKVLAVLLSALILGFAFGAVSNSGSSPITSAASVV
                     FSPLQKLSARLAEAMKDFNAGFVSADYYRKQIDELKLEIEKYKEQLVEYEKQKRKLQS
                     YEAMLGVKEEHKDFKLSAAEIISRDPSDSFFSFVIDKGSLHGIEVDMPVIYGKQLIGV
                     IKEVTPSTALVKTILSPEINVGAYEIRTFEDGYTRGLVRDGKSGLMEMSGLTGFSAIS
                     KGGLVCTSGVGGLYPRDLIIGSVTEIYTDNITTAGAAVLEPGLDFTELTDVFVITDFS
                     GRQTGK"
     gene            complement(3612..4628)
                     /locus_tag="PLD98_02720"
     CDS             complement(3612..4628)
                     /locus_tag="PLD98_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQA05196.1"
                     /translation="MLFSQDIGIDLGTANTLVFVKGKGIVIREPSVVAVDIRSIPHRV
                     VAVGSAAKQMIGKTPGSITAIRPLKDGVIADFEITAEMLRIFIKKAVSNSLFNRARVM
                     ICIPSGVTEVERRAVHDAARSAGARYVSLIEEPMAAAIGSGLPVAEAKGSMIVDIGGG
                     TSEVAVISLGDIVTSRSARVAGDNFDDAIVNYIRRKYNLLIGESTAEEIKIKIGSAYP
                     YEGEGALDIKGRNLLDGLPKNIEITSEEVREALSDPVNQILDTIKSTLEKTPPELAAD
                     IVDNGIMLTGGGALLRGLDKLIASETQMPVHVAENPLDCVAIGTGICLEKDILNLSNK
                     KRLI"
     gene            4955..5383
                     /gene="rplM"
                     /locus_tag="PLD98_02725"
     CDS             4955..5383
                     /gene="rplM"
                     /locus_tag="PLD98_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQA05197.1"
                     /translation="MSTYMPKAGDITRKWYVIDAEGKTLGKVAAAAASILRGKHKTTF
                     APHADCGDNVVIINAAKVVLTGKKLQQKKYYRHTGYIGHLKEVSYATLMRTKPEFVVE
                     KAVKGMLPNNVIGRNAARRLRVYRDANHKQTAQKPEVWTV"
     gene            5401..5799
                     /gene="rpsI"
                     /locus_tag="PLD98_02730"
     CDS             5401..5799
                     /gene="rpsI"
                     /locus_tag="PLD98_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQA05198.1"
                     /translation="MYETNPFFYGTGRRKKSVARVRLYAGTGKITINERDIDEYFGLE
                     TLKVIVRQPLELTNTTDKFDIVCRVSGGGVTGQAGAIRHGIARALLEYNEELKPVLKS
                     AGFLTRDPRMKERKKYGLKGARRAPQFSKR"
     gene            complement(5844..6092)
                     /locus_tag="PLD98_02735"
     CDS             complement(5844..6092)
                     /locus_tag="PLD98_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05199.1"
                     /translation="MIECLKVYARISKKAFIEVINHPNFKLENVLHYRKIADFFGEEI
                     REFMLKNRYSAEYLLKEYAMNDDKSERLYFIIFSKLKK"
     gene            complement(6169..6432)
                     /locus_tag="PLD98_02740"
     CDS             complement(6169..6432)
                     /locus_tag="PLD98_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05200.1"
                     /translation="MQKYISWGGNMERIKYFSNSSFPVLINDSIIESVLNEYVQNRKL
                     RNIDDMTEIYNIKKYFQSGLCPKELIEVYGETVNTVCGGGKIF"
     gene            6593..7096
                     /locus_tag="PLD98_02745"
     CDS             6593..7096
                     /locus_tag="PLD98_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA05201.1"
                     /translation="MKNLIIINGTMGVGKTAVSKELQKLLPDCVFLDGDWCWDMSPFI
                     VNHETKEMVLDNISYLLNNFISCSVFENIVFCWVMHEQSIIDNVLSRLKLTDCKVHKF
                     SLVCSEEALISRLKNDIAAKIRSEDIIERSVPRLKNYDKMDTVKIDVSHISAKDAAEI
                     IFRHIMQ"
     gene            7178..7534
                     /locus_tag="PLD98_02750"
     CDS             7178..7534
                     /locus_tag="PLD98_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HQA05202.1"
                     /translation="MGRAESICKMSFSKVYPYYLAKAEKKGRTKAEVDEIIRWLTGYS
                     QEELEAQIEKETDFETFFNEAPMLNPARSLIKGVICGVRVENIEDPLMRNIRYLDKLV
                     DELAKNKTMDKILRKE"
     gene            complement(7613..>17548)
                     /locus_tag="PLD98_02755"
     CDS             complement(7613..>17548)
                     /locus_tag="PLD98_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05203.1"
                     /translation="PIGNHDRQYAGTFDGQGFTISNVYVNSTSVDRGLFGKITTSGVI
                     KNVNVNNITIAGTRDYSGGLLGVAVGGTISNCHVTGINISAGRAQTGGLIGQVEGAAV
                     ITDCSVTGTSSMKTIVGTNNTGGLVGYIKANNVQFRNCRVENLNISSTSSNSRIAGAI
                     GHADYRFDMYDTIVKGVNVTGTRYMGGLAFAVKNGSFIERCMVLDSTITSTQTSATTD
                     VEIGGLIGVVTSDVANTIQYNAVYNTTVSSAGRMSGGFIGKIEAARHFISENYSAATL
                     NLRAAPTRAGTLVGYVANAELNDLFYVTASGQNAIGEQVSSSTVTNIRGVTAAEATNG
                     ALLSYLNNLTNIWKTGNDGYPVIDGNLAVAPSKDASGYFLIGSAFQLEWFQKHVNAGN
                     TSASAKLVADINLNNKKMDTIGNNNVRYAGTFDGQNFTISNLYISKTGNDKGLFGSTA
                     PTAVLRNLKLKNVYINTNGSYVGGLVGLLRGTVSNITVEGMNINGASYVGGVIGQSEA
                     STQLNNITVIGETGAKTIKGTGSFVGGVAGNIKATMPVNNVIVKNVDFTANEKAGGVF
                     GDSDGGTVRISEALVENVNISVNRYAGGVIFSVRNASTVDKATVRNVRITGGAELGGI
                     SGTSNGNPVSVTNSLVENFTCVSNNERTGGIIGYINNKATITDTIIKDSSLTGTRYIG
                     GVSYSLKDGSFISRVGVINVTINATNTGTGNEFCVGGLIGVVGWSTANTIQYNYVSNV
                     TITGVGRMIGGFIGKMDNANHAIAENYADASINNNTKIYVGSFLGYKSSGNVVNNFAR
                     ANGFPRTGGASAGASGDVSGITEVSLADIRNGSLTEMLNDITDLWAQYNDDYVNGFPV
                     IANAEPPALVNGYYEIKNTFQLEWFRNFVNDGNVNANAKLIADINIRNKEWIPIGTDS
                     RRYNGIFDGNNHSISNFKIQPATYDRGLFGSTNPNAVIKNLTLRNVSLNSTSEYVGAV
                     VGLLRGKVENVTVEGVNIVAKAQVGGIAGQIESNGSIKDSSVIGLPGMNVIRGTGNNI
                     GGLAGNIKTTSYITNVSSKDLVINANERAGGISGDTDGGTGHFDTVNVDNVTISVVRY
                     GGGVVFGLRNASSVNNAIVKNVTINGAEELGGIAGCTSGSEVKITKSLVENYTGNSTS
                     ERTGGIIGYINSTASISDTIIRNSSIKGSRYIGGVSYTAMNGTKIIKVGVENVTITAT
                     NTNASTDICVGGLIGVVGWNTGNTIQYSYAQNVTITATGRMVGGLIGKIDTANHQILE
                     NYANANISGNVSTYGGSFVGYRTSGTFANNFAKPISGLPLAGYGASTGISEVSLQDIK
                     NGNLTEMLNNVTDIWAQYNNDYENGYPVIGNAEPPQLVNGYYEIETAFQLEWFKNYVN
                     AGNTNANAKLLNDINLRNKDWYPIGNNDRRYTGTFDGQGFTVRNVLITTTSVDRGFFG
                     KLGSGGVVKNLNLENVQITTNRNYTGGLVGVLNNASVINCHVRGIKITNTGQYIGGLV
                     GQTEGTITIADSSVTGTETMNTIVGSSYVGGFVGRNDTSNANFMNNVVNGVNVSATAT
                     DSRAGGFAGYNEGTIFAYDNLVKNTDITGGRYLGGFTFAIRDDSVVERNGVVNTKVTS
                     TYNSPATDIEIGGFAGVIVSNRVGRIQFNYVVDSEITSYGRVSGGFAGKVESARHKVS
                     ENYSDTLVNIIGTPTRTGTAFGWLTNGEISTLYYVPKAGQNAIGEYNTTPLINNVTPI
                     EKAEVKNGSLLEKLNDITPIWEASEDGYPVPQKDLVSAPEKDADGYYLIYNSYQYEWV
                     TRFVNSGNPNTRIKLMADINLNGKNIDPLGTNSSRYSGIFDGNGFEISNLYISKTAND
                     IGLLGSTTNAVVVKNLTLRNVDISTTASYVGAVAGLFRGRMENVHVIGLNIRGTSSVG
                     GLAGQTNENVTLINCSVTGTETMKTISGSNSNVGGVIGYVDTSGLTLTDVSVKGVNIS
                     AGTERAGGIAGDTSPKMTFTNISVEDTSVTAPRYIGGLVFYLRNGSTVNGATVKNVTL
                     TGTSAVQAEIGGISGSNSGTASTINNAAVENLTINTPGECVGGILGLANSAIIITNST
                     VKNSTLNASRYMGGISYNLGYGSSLTKSGVENVTLNASNTSTTNDIAAGGLVGVVGWG
                     TANTVQYNYVDRAAVNAVGRMTGGLIGKIDNANHSVSYNYVNDTSVTSGAIYTGSLVG
                     YRTSGTFSNNFVVGSSALPLVGSDKVGTPGNVAGTALAEVFTGDIETSETPVLLSYYI
                     SATDFIKSFQNQNVVFLKEGIFSELYDEISAILVSIAAELARRLDSEISVLKEFYDEY
                     GMICIFNYELIGTHLGTVTNAIAQYVTLPPETQANYEFYLQLIDDFNDYLANHGYNSF
                     YEATVGYTVRKVREDDIARYESPRDDFPVTYEKMQNVVSRLDALLLSDDFATLAGLES
                     SLSRKIKDILAEELYNNEIINDLMSSLYPKLIEELESAIEENAEIDLKVTKITFRGRA
                     RDLVDDLLRKFNLWVYPTYLADQIDPKYAAVRSVLYSADKDWSKVNFDDPEAPNYLDW
                     GELDKEGFIDALSNSLMGIYPVLRAALTANSFNDRKNSSLIANIDVSIRLQINAVDAY
                     DRAIVPLLEMLNITGFMPAAEYNNLTSVKGLLENILNPLLTWIEEEIPTAPFSNIMEM
                     LPNIAYSLQFGLVESWLRAIKTTIYYDIDGVFDYWLGDIDFDIANGEYAFDAFEMLSE
                     EEDDILYGADLSSINGILKMVLKQMEIDVELPPINNKYFASLGSVVYLNSATRQQTRG
                     YIQGDSAKVAYVILRYIFNILSDEELVRAILGEEEGEDKLDGTIGDIVRNIGNSPEDA
                     IAAIVELCNPVEYDVRGFDYGGPFEGEYPKVQYSQYWTKQQAKFITDHLDTYIDNILK
                     LLGLKPAGELLREKLGELYTNDTLTNIILGARDALESLDSDGKILKVLDVDITPWYDV
                     DYGYDWGFEDGDKDAFLRALCDALSPLNNAIGLFLADMDYNIMDGEITIKGYNGYKNA
                     IVPLLEAIGCDYDEILTGEEYLAAVEADPNSMIELIIKPIFGLLERLYAAPTETIFSI
                     LPNLIYFLDSGVLDTAILNLVQPILVVVDTIRPIYSLEFEFSASEYIKEAIRELVAEN
                     GITLPDINLSDMLKAYAKPKTNVHGEEIYVIDADNEDVLTVMLRYIVATVFHPANAEL
                     IKSEVIDEAGLSGDSAAIIKAAFETFGELINKENGADQVLGMFYYIFYGTDLGFGHGA
                     NFLDNLNENWRKALEMLSNSESEFLRNMAGALNELMKDYLVGIIDEGGLAPNGVLRFF
                     QRIAEFFRNFLNMLRNLFK"
BASE COUNT         4113 a         4198 c         4458 g         4779 t
ORIGIN      
        1 aaaataattt cacttctata ttacccgaat ttataaaaaa ggcaataatt atgttaaatt
       61 ttgtataata cgctacggca gaagggtgtt gaaaggctgc tgcggaacca cgctcttcat
      121 ggtgccgaag taatattcgt aaatatccgc ggcgaccttg gccgttgccg taccgctgtc
      181 cacattctct ataagaacca caacggcgat ttcggggttt tcatagggag caaagctgat
      241 aacaaagccg ttgttgccct taacagccct gccgtttact attttgaccg tctgagaggt
      301 acccgtctta gccgccgcct gaaccttcag gtccttaaag gtgctctgga gcgaaccttg
      361 tgttacggct ctgagcatgc cctccttgac ggtggcgaat gtggatgccg aaatatttgt
      421 tttaaacgca acggcgggat ctttcttcat gatggtggag gatttgtcgg gagtcttaac
      481 ctccttgatt atatggggaa cataccttgt gccgccgttc gctatcgctg cgcagtagtt
      541 tacaagctgc aggggagtaa agaggttgtc cgactgaccg atggacgcct gaatggtatc
      601 gccggggagc cacagcttgc caaggctctc cctgtaaggg atacccgcca gaacgccctc
      661 cgcttcgggc agctctatgc ccgttttgga gccgagtccg aacgctgtgg cgtattcgtt
      721 catcttgttt atgcctaaaa tgtccgccac attgtaaaag taaatgttgc aggatttttc
      781 tatcgcctcg accacatttg tccttccgtg gcttttaaga cagcggaagt ccatgccctt
      841 gtagtggaaa acgctgttgc agatatactg gtcgtacttg tttaccgtgc ccgtctccaa
      901 ggcggcaatc gccacaaggg gcttaaatgt ggagccgggg gcgtatgtgc tctggagtac
      961 cctgttccac aagggagcgg cagggtcgga agcgagcttt gagtagtttt cgtaataggt
     1021 tgaaaggtcg tagccgggat atgaagcgct ggccagcagc tcgcccgtat ttacatcaat
     1081 gaccgccaca gcgcccgcgg cctcaagacc cttttgagcc gtcagctcct tgattctctt
     1141 ttcaagagcg atttccgcag ccttctgaag tcccgcgtca atggtcagta tcaccgtttc
     1201 gcccttttcc ggctcctcta tgtagctctc tttgagattt ccgtccgtat ctctgcttat
     1261 gaccttatat ccgtcggcgc ctttaaggta ttcctccatc agcttttcga cgccgctctt
     1321 gccgtaaata tcgtcaaggt cgtatgagcg tttttcgaga gccctttttt cttcctcagt
     1381 gagattcggg tccttcagct tttgctccaa ggcgtttttg gcgttttcat actcgctctg
     1441 ggaaatcgcg cccacagtgc ctataagatg cgccgcaagg ctcttttcgg ggtattctcg
     1501 gtatgttacc acctcatgct caacgcccct gtaaaagtcg ctgcgctctt ttatggcggc
     1561 gattgtggcg gagcttacat cctcggcgaa aacataaggg ttcgttatcg aaaagccctg
     1621 ctttcttaaa gacacgcata ccgaggcgat ttttatcgcg tcctcatcgg aatagtcctg
     1681 caaagagaaa tactctttaa gagcgtcaaa gcagttctgc gcggtggcgt agtgattgag
     1741 attcagcata tcgggggatt taaggtattc tatgtagctg tcgctgttct ccttaaagct
     1801 gaccgagccg tcggggttga gaataagcgg cagattgtcg ttccactcct cccccttgct
     1861 ttcgagaagc tttattaaag atatgataat ctcgtttctc tcggactgat ttttaagaga
     1921 ggggaaatgg gcggcggaga aaacaatcct gttgccctga cggtttgtgg caaggacatt
     1981 gccgtttctg tcaaggattt cgccccttgc gccctccacc ttcagggtat agacatccac
     2041 atttttgctt gcggcaatct cgtcgcgatt tataagctga atcctgaaaa gctgaacgac
     2101 aaagatgccg agtatagaga atatggctat aagggcggat gtaacccttt ttaagtttgt
     2161 ctttttggaa cttaacattt tttcaccgta gtatggaact aaattttgtc caaatcatcg
     2221 cttgtcttaa aatgctttga aatatatcga atgagaagat aaaacacagg aagaaaaagc
     2281 gccgttaaag cgaggggaga aaagagtttt aacgcctcgc cgtaaatctt tcttaaaaag
     2341 atgcatctga aaagcgtgta taatccgccg aaaaagacag tgagtataag ggcggataca
     2401 aggttgtttc ttaaaatata ccttaccaaa agcccgcagg caaagcccat aaggcaaaag
     2461 aaaatggcgt taaagccgtc gggcaaaggg gagctgatgt cgtagataat tcccgccagc
     2521 gcgccgtaaa gggctcccga aagctccctt tcaaaaagcc caaggcagat tacaagggga
     2581 atgaaggcat aaaaggaaga aagtatggga actgatggcg ttgtgtgcag cactgaaacc
     2641 gcaatcacaa ggaccgccaa aatcgccctg tatatatgca tctgcctgtt gtttttaaag
     2701 ggcaaagcaa tcgtccttcc ttttagttac tttcccgtct gccttccgct gaagtcggta
     2761 attacaaaaa catcggtaag ctctgtaaag tcaagccccg gctcaagcac cgcggcgccc
     2821 gcggtcgtta tgttgtcggt ataaatctct gtgacggagc ctattataag gtcgcgggga
     2881 taaagtccgc ccacgcccga agtgcataca agtccgccct ttgagatggc ggaaaagccg
     2941 gtaaggcccg acatctccat aagtccgctc ttgccgtcgc ggacaagacc ccttgtgtag
     3001 ccgtcctcaa aggttcttat ctcatatgcg cccacattga tttcggggct taaaatggtc
     3061 tttacaaggg ctgtggaggg ggttacctcc ttgataacgc ctatgagctg cttgccgtaa
     3121 atgacgggca tatccacctc aatgccgtgg agggagccct tgtctatcac aaaggagaaa
     3181 aagctgtctg atgggtcgcg ggagataatc tccgccgcgg agagcttgaa atccttgtgc
     3241 tcctccttaa cgcccagcat tgcctcgtaa gactgaagtt ttctcttttg cttttcgtat
     3301 tccacaagct gctctttgta tttttctatt tcgagtttga gctcatctat ttgctttctg
     3361 tagtagtcgg cggacacaaa gcccgcgttg aaatccttca tagcctcagc cagccttgcg
     3421 gacagctttt gaaggggaga gaacacaaca gaggcggcgc ttgttatggg agaggagccg
     3481 ctgttgctga cagctccgaa ggcaaagcct aaaataaggg cagataacag tacagccagc
     3541 accttgaatg cggttgtgcg tatgtaggtt ttcatttgat aatcctccgg cgccaagaat
     3601 cggcatttgc cttaaatcag tcgctttttg ttcgaaagat ttaaaatatc cttctcaagg
     3661 caaattcccg ttccgattgc aacgcagtcc agggggttct ccgccacatg cacgggcatt
     3721 tgagtctcgc ttgcaatcag cttgtccaga ccccttaaaa gcgctccgcc gcctgttagc
     3781 ataatgccgt tgtccacaat gtcagccgcc agctcggggg gagttttctc aagggtggac
     3841 ttaattgtgt caagaatctg attaacgggg tcgctgagag cctcccttac ctcctcggag
     3901 gtaatctcaa tattcttcgg cagaccgtca agcaggtttc tgcctttaat gtcaagggcg
     3961 ccctcgccct cgtacggata agcggagcct atctttatct taatctcctc ggcggtgctc
     4021 tcgccgatta aaagattgta tttccttctg atgtagttga ctatcgcgtc gtcaaaattg
     4081 tcgcccgcaa cccttgccga gcgtgatgtt acaatatcgc cgagggaaat aacggctacc
     4141 tctgaggtgc cgccgccgat gtccactatc atgctgccct ttgcctcagc gacgggcaga
     4201 ccggaaccta ttgccgcggc catgggctcc tcaattaaac ttacatacct tgcgcccgcg
     4261 cttcttgcgg cgtcgtgaac cgccctgcgc tcaacctcgg taacgcctga gggaatacaa
     4321 atcataaccc tcgccctgtt gaacagagag tttgatacag ccttctttat aaagattcta
     4381 agcatctccg ccgtaatttc aaagtcggcg ataacgccgt cttttaaggg tctgatggcg
     4441 gtaatggagc caggagtctt gcctatcatc tgctttgcgg cgctgcccac agccacaacc
     4501 ctgtggggta tggagcggat gtccaccgcc acaaccgacg gctcccttat tacaatgccc
     4561 ttgcctttta caaacacaag ggtattggcg gtacccaaat ctattccgat gtcctgtgaa
     4621 aataacatat tattccccca aacggatatt gccaatggcg acaaattcac agcttaatta
     4681 tatattgttt gacctctttt ctcaacatat tttaactttt ttaatatttg aagtattttc
     4741 atgccgaatc ggagaaaata aaatgaaaat catttgcgag gaaaaaagag aaaaacatat
     4801 ggattttaaa gaaaatcgga gatactaaaa ggtaaataaa aatttatttt aaaatgtatt
     4861 gacacccgaa attacctatg ctaatataat cgggcacgaa aaaggcttta aagttaaagt
     4921 attagcttta cgaatttaat cggaggtata aaatatgtca acttatatgc ccaaagcagg
     4981 tgacatcacc cgcaaatggt atgttattga cgccgaagga aagactttag gcaaggttgc
     5041 ggctgccgct gcttccatcc ttcgcggcaa gcataagacc acatttgctc ctcatgccga
     5101 ctgcggcgac aatgttgtta taatcaacgc cgcaaaggtc gtcctgaccg gcaagaagct
     5161 ccagcagaag aagtattatc gccacacagg ctatatcggc cacctcaaag aggtcagcta
     5221 cgcaactctt atgagaacaa agcccgaatt tgtcgttgag aaggcagtta agggcatgct
     5281 tcccaacaat gtaatcggca ggaacgccgc tcgccgcctg cgcgtttaca gggacgcaaa
     5341 tcataagcag acagctcaaa agcctgaagt ttggacagtt taaggggagg gcaaaagatt
     5401 atgtacgaaa ccaatccatt cttttacgga acaggaagaa gaaagaagtc tgttgcccgt
     5461 gtaaggctct atgcaggcac aggtaaaata acaataaacg aaagggacat cgacgagtat
     5521 ttcggtcttg agacactgaa ggttatcgtt cgtcagcccc ttgagcttac aaacacaacc
     5581 gacaagtttg acatcgtatg ccgagtatca ggcggcggcg taaccggtca ggcaggcgcc
     5641 atcagacacg gcatcgcaag agcgctgctc gagtacaacg aggagttaaa gcccgttctc
     5701 aagtccgcag gtttcctcac ccgcgacccg agaatgaagg agagaaagaa atacggtctc
     5761 aagggcgccc gtcgtgctcc ccagttctca aagagatagt tttcaaatca accgtcaaag
     5821 gaatcccctt tggcggtttc tttttatttt ttgagctttg aaaaaattat gaaatataaa
     5881 cgctcacttt tgtcatcatt cattgcatac tcttttaata gatattcagc tgaatatcta
     5941 ttttttaaca tgaactctct tatttcttca ccgaaaaaat ctgctatttt tcgataatgc
     6001 aaaacatttt ctagtttaaa attagggtgg ttaatcactt caataaaagc tttttttgat
     6061 attcttgcat acacttttaa acactcaatc aattcccaga aatcctcact gtatagcagc
     6121 ttatctagat cgataaagga attataaaaa ctttcatcat ccgaccggtt aaaatatttt
     6181 gccacctccg caaacagtgt ttactgtttc cccatacact tctattagct ctttaggaca
     6241 taaaccgctt tggaaatact ttttaatatt atagatttcc gtcatatcat caatatttct
     6301 gagttttcgg ttttgaacat actcattaag cactgactct attattgagt cattaatcaa
     6361 tacagggaaa ctactatttg agaaatactt tattcgctcc atatttccac cccacgatat
     6421 atacttctgc ataagcaaaa acgccatatt gttgcaatac ttacaatata cgcggataaa
     6481 ttcaactatt atcgaggcgg catataccaa gcaaaactag attatttcag agccaatgtg
     6541 tataatattt gagcaacccg aaatatttag tttaatctgt ggcgagagga aattgaaaaa
     6601 tttgattatt ataaacggta caatgggtgt cggcaagacg gctgtaagca aggagctgca
     6661 aaagctgctc cctgactgcg tttttcttga cggtgattgg tgctgggata tgtcgccctt
     6721 tatagtgaac cacgaaacaa aagagatggt gctcgataat atcagctacc tattaaacaa
     6781 cttcatttca tgctcggtat tcgagaatat cgttttctgc tgggtaatgc atgagcagag
     6841 cataattgat aatgtgctaa gcaggcttaa attaacagac tgcaaagtac ataaattctc
     6901 gcttgtctgc tcagaggagg cgctgatttc ccgccttaaa aatgatattg cggcgaaaat
     6961 cagaagcgaa gatataattg aaagaagcgt tccgagactt aagaattacg acaaaatgga
     7021 caccgtaaaa attgatgtaa gccatatttc agcaaaagac gccgcggaga taatttttag
     7081 acatataatg cagtaatatt aaaaccgtca aagaaattct ttggcggttt ttcttttatg
     7141 gtataataaa ctcagaaacc gagaaacgag gtgcaatatg ggcagggcag aaagtatctg
     7201 caaaatgagc ttctctaagg tttatccata ttatctcgcc aaggcggaaa agaaagggcg
     7261 caccaaggca gaggtggacg aaatcatccg ctggctgaca ggctacagtc aggaggagct
     7321 tgaggctcag atagaaaaag aaacagactt tgaaaccttt tttaatgagg cgccgatgct
     7381 caatcccgcc cgctccctta taaaaggagt aatctgcggc gtcagagtgg aaaacataga
     7441 ggaccctctt atgcggaata tccgctatct ggacaagctt gtagacgagc ttgctaagaa
     7501 taaaacaatg gataaaatcc ttcgcaaaga ataaaagcag gggaaaccct gctttatgtt
     7561 taaatccacc gcaaagaatc agccgcccgc gttagcgggc ggctttgccg ttttacttga
     7621 aaaggtttct taacatattg aggaagtttc tgaagaactc ggcgattctc tggaagaacc
     7681 tcagtactcc gttgggagca agaccgcctt cgtcaattat tccgacgagg taatccttca
     7741 tgagctcatt cagcgcgcct gccatattcc tcaggaactc gctttcggaa ttggagagca
     7801 tctcaagagc cttcctccag ttctcgttga ggttatcaag gaagttggct ccgtggccga
     7861 agcccaaatc ggtgccgtag aagatgtagt agaacatacc gagcacctgg tctgcgccgt
     7921 tctctttgtt gataagctcg ccgaaggttt caaaggctgc cttgatgatt gccgcgctgt
     7981 cgccgctgag acctgcctcg tcgatgacct cgctctttat aagctcagcg ttcgcgggat
     8041 ggaatacagt cgccacaatg tatctgagca ttaccgtcag aacatcctca ttgtccgcgt
     8101 ctattacata gatttcctcg ccgtggacat tagtcttcgg ctttgcgtat gccttgagca
     8161 tgtctgagag gttaatgtcg ggcagcgtaa tgccgttttc ggcgaccagc tctctgattg
     8221 cctctttgat gtactcggag gcggagaact cgaactcgag gctgtaaatg ggtctgattg
     8281 tgtctactac aaccagtatc ggctgaacga ggttgagtat agcggtgtca agcacgcctg
     8341 agtcaaggaa gtagatgagg ttcggcagta tggagaagat tgtctctgtg ggcgcggcgt
     8401 atagtctctc aagcagtccg aagatgggct tgatgataag ctcaatcatg gagttcgggt
     8461 ccgcctctac cgctgccaga tactcctcgc cggtgagaat ttcatcatag tcgcagccga
     8521 ttgcctcaag gagcggaacg attgcgttct tgtagccgtt gtagcccttg attgtaatct
     8581 cgccgtccat gatgttgtag tccatatcgg caaggaaaag accgattgcg ttgttcagcg
     8641 gtgagagcgc gtcgcacaaa gctctgagga acgcgtcctt atctccgtcc tcaaagcccc
     8701 agtcgtagcc gtagtcaaca tcgtaccacg gtgtgatgtc cacatcaagg accttgagaa
     8761 tctttccgtc gctgtcaagg ctctccagag cgtctcttgc gccgaggatg atgtttgtca
     8821 gggtgtcatt ggtgtaaagc tcgccgagct tttctctcag cagctctccc gcgggcttga
     8881 gacccaggag tttcagaata ttgtcaatgt aggtgtcaag atggtctgta atgaacttag
     8941 cctgctgctt tgtccagtac tggctgtact gtaccttcgg gtactcgccc tcgaagggtc
     9001 cgccgtagtc aaagccccgt acatcgtact ctaccgggtt gcaaagctca acaatggctg
     9061 cgattgcgtc ctcggggcta ttgccgatgt tccttacgat gtcgcctatg gtgccgtcca
     9121 gcttatcttc gccttcttct tcgccgagta tagcccttac gagctcctca tcgctgagaa
     9181 tgttgaagat gtatctgagt attacatagg caacctttgc gctgtcgccc tggatgtagc
     9241 ctctggtctg ctgccttgtg gcggagttga ggtagacaac cgagccgagg gacgcgaaat
     9301 acttgttgtt tatcggcggc agctcaacat caatctccat ctgcttaaga accatcttca
     9361 gaatgccgtt gatgctggag aggtccgcgc cgtagagaat gtcgtcctct tcctcggaga
     9421 gcatctcgaa agcgtcaaag gcatattcgc cgttggcaat atcgaagtca atgtctccga
     9481 gccagtagtc gaacacgccg tcgatgtcgt agtagattgt ggtcttaatg gctctgagcc
     9541 aggattccac aagaccgaac tgcagcgagt aggctatgtt cggaagcatc tccatgatgt
     9601 tggagaaggg cgctgtcggg atttcttcct caatccaagt aagcagcgga ttcaggatgt
     9661 tttcaagaag tccctttacg cttgtgaggt tgttgtactc tgcggcgggc ataaagcctg
     9721 tgatgttaag catttccaaa agcggtacta tcgctctgtc gtaagcgtca accgcgttaa
     9781 tctgaagcct gatgcttaca tcgatgttgg ctataagact tgaattcttc ctgtcgttga
     9841 aggagttggc ggtaagagcc gctctgagta cggggtagat acccatgagg ctgtttgaga
     9901 gagcgtcaat gaagccttcc ttgtccagct ctccccagtc gaggtagttg ggagcttcgg
     9961 ggtcatcaaa gtttaccttg ctccaatcct tgtctgcgct gtagagcacg gacctgaccg
    10021 cagcgtactt cgggtcaatc tggtctgcaa ggtaggtcgg gtatacccag agattgaact
    10081 ttctcagaag gtcgtcaaca aggtctcttg ccctgcctct gaaggttatc ttggtgacct
    10141 taaggtctat ttccgcgttc tcctcgattg cggattcgag ctcctcgatg agcttcggat
    10201 agagcgagct cataaggtca ttgattattt cattgttgta aagctcttcg gcgaggatgt
    10261 ccttaatctt acggctgagg ctggactcaa gacccgcaag tgtcgcgaag tcgtcgctga
    10321 ggagcagagc gtcaagtctg cttaccacat tctgcatctt ctcgtaggtt acggggaagt
    10381 cgtctctggg gctttcgtac cttgctatgt catcctcgcg aacctttctg acggtatagc
    10441 cgacggttgc ctcatagaag gagttgtagc cgtggtttgc cagatagtcg ttaaagtcgt
    10501 cgatcagctg gaggtagaac tcatagtttg cttgagtttc gggcgggaga gttacatact
    10561 gagcgattgc gtttgtaact gtgccgagat gtgtgccgat aagctcgtag ttgaatatgc
    10621 atatcatgcc gtattcatcg tagaactctt taagcacact gatttcgctg tcaagtctcc
    10681 ttgccagctc agccgcgatt gagacaagga ttgcggaaat ctcatcgtaa agctctgaga
    10741 agattccctc tttaaggaat acaacattct ggttctggaa ggacttgata aagtctgttg
    10801 cggatatgta gtagctgagc agtacgggtg tctcggaggt ttcgatgtcg cccgtaaata
    10861 cctctgccaa agcggtaccc gctacatttc cgggagttcc gaccttgtcg gagcccacaa
    10921 gcggcagcgc actggagcct acaacgaagt tgttggagaa ggtgccgctg gttctgtagc
    10981 ctacaaggct gcctgtgtag atggctccgg aggtgacgga tgtgtcgttt acatagttgt
    11041 agcttacact gtggtttgcg ttgtcaatct tgccgatgag accgcccgtc attctgccca
    11101 ccgcgtttac ggcagctctg tcaacatagt tgtactgaac ggtgtttgcg gtgccccagc
    11161 cgacaacgcc cacaagtccg ccggctgcta tatcgtttgt ggttgatgtg ttgcttgcgt
    11221 taagcgttac attctcaacg cccgactttg taaggcttga gccgtagccc aggttgtagg
    11281 agatgccgcc catgtacctt gaggcgttaa gggtggagtt cttaacagtg ctgttggtaa
    11341 taatgattgc gctgtttgcg agacccagaa taccgccgac gcattcgccg ggtgtattga
    11401 ttgtaaggtt ctcaaccgca gcgttgttaa ttgtggaagc tgttccgctg ttggatccgg
    11461 aaataccgcc gatttctgcc tgaacggcac tggtgcccgt aagcgtaaca ttcttaaccg
    11521 tcgcgccgtt tacggtcgag ccgtttctga ggtagaacac aaggccgcct atatagcgcg
    11581 gtgctgtgac actggtgtcc tcaacggaaa tgttcgtaaa ggtcatcttc gggctggtgt
    11641 cgcctgcgat gccgcctgct ctttcggttc cggcggagat gttaacgccc ttgaccgaaa
    11701 catccgtaag ggtcagtccg gaggtgtcaa catatcctat gacgccgccc acattggagt
    11761 tggagccgct gatggttttc attgtctcgg tgcctgttac ggagcagttg ataagggtta
    11821 cattttcgtt cgtctgacct gcaagtccgc cgacggacga tgtgcccctg atattaagtc
    11881 cgattacatg gacattctcc attctgcctc tgaacagacc ggctacggcg cctacatagc
    11941 ttgctgttgt tgaaatatca acatttctga gtgtcaggtt ctttaccaca acagcgttgg
    12001 tggtggagcc taaaagaccg atgtcgttag ctgtcttgct gatatagagg ttagaaatct
    12061 caaagccgtt gccgtcaaag atgcccgaat atctgctgga gtttgtgcca agcgggtcaa
    12121 tgttcttgcc gttaaggtta atatcggcca tgagcttgat gcgtgtgttc gggttgccgg
    12181 agtttacgaa ccttgttacc cactcatact gataggagtt gtagatgagg tagtagccat
    12241 cggcgtcctt ttcgggagcg gatacaaggt ctttctgcgg aacgggatag ccgtcctctg
    12301 aagcctccca gatgggagtg atgtcgttaa gtttctcaag cagcgagccg ttctttacct
    12361 ccgccttttc aataggagtt acattgttga taagcggagt ggtgttgtac tcgccgattg
    12421 cgttctgacc ggctttggga acatagtaga gggttgagat ttcaccgttt gtaagccagc
    12481 cgaaagcggt gcccgttctt gtgggtgtgc cgatgatgtt gactaatgtg tcggagtagt
    12541 tttcacttac cttgtggcgg gcggattcaa ccttgccggc gaagccgccg gatactctgc
    12601 cgtaagaagt gatttcggag tcaacaacat agttgaactg aattctgccc acgcggttgc
    12661 tgactataac gccggcgaag ccgcctattt caatatccgt tgcggggctg ttgtaggtgg
    12721 aggttacctt tgtgtttaca acgccgttgc gctctaccac ggagtcgtct ctgatggcga
    12781 aggtaaagcc gcccaggtac ctgccgcctg tgatgtcggt gttcttgacc aagttgtcat
    12841 aggcgaatat cgtgccttca ttgtagcctg caaagccgcc tgcgcgggaa tcggttgcgg
    12901 tggcggagac attgacgcca ttgaccacat tgttcatgaa gttcgcgttg ctggtgtcgt
    12961 tcctgcccac aaagccgccg acataagagc tgcccacgat ggtgttcatg gtttctgtgc
    13021 ccgttactga cgagtcggcg attgtgattg tgccttccgt ctgacctaca aggccgccga
    13081 tgtactgccc tgtgtttgta atcttaatgc ctctgacatg gcagttgata accgaagcgt
    13141 tgttaagtac gcccacaaga cctcctgtgt agttacggtt tgtggtaatc tggacatttt
    13201 cgagattcag gttctttaca acgccgccgc tgcccagctt gccgaagaag cctctgtcaa
    13261 ccgatgttgt ggtgatgagg acattcctta cggtaaagcc ctgaccgtca aatgtgcctg
    13321 tgtaccttct gtcgttgttg cctatcgggt accagtcttt gtttctcagg ttaatgtcgt
    13381 tcaagagctt tgcgttggcg ttcgtgttgc ctgcgtttac atagtttttg aaccactcaa
    13441 gctggaaggc ggtttcaatc tcgtagtagc cgtttaccag ctgcggaggc tcggcgttgc
    13501 cgattacggg atagccgttc tcatagtcgt tgttgtactg agcccagata tccgttacat
    13561 tgttaagcat ttccgtaagg ttgccgttct tgatatcctg gagggaaacc tctgaaatgc
    13621 ctgtggaagc gccgtagcct gcgagaggca agccgctgat gggctttgcg aagttgttgg
    13681 cgaaggtgcc gctggttctg tagcctacaa agctgccgcc gtaagtgctt acattgccgc
    13741 ttatgtttgc atttgcgtag ttttcaagaa tctgatggtt ggcggtgtca atctttccta
    13801 taagaccgcc gaccatcctg cctgtcgcgg taatggttac attctgagcg tagctgtact
    13861 ggattgtgtt gcccgtgttc cagccgacaa caccgataag gccgccgacg cagatgtcgg
    13921 ttgacgcgtt ggtattggta gccgtaatgg taacattttc aacgccgacc ttaataatct
    13981 tggtgccgtt catggctgtg taggaaacgc cgcctatgta gcgggaaccc ttaatggagg
    14041 agtttctgat tattgtgtcg gagattgaag cggtgctgtt gatgtagcct ataataccgc
    14101 ccgttctctc ggatgtggaa ttgcctgtgt agttttcaac aagggacttt gtaatcttga
    14161 cctcgctgcc ggatgtgcag cctgcgatgc cgcccagctc ctcagcgccg tttatggtta
    14221 cattctttac tatcgcgttg ttgacgctgg atgcgtttct cagaccgaac acaacgccgc
    14281 cgccgtatct tacgaccgag atggtgacat tatccacatt gacggtgtcg aagtggccgg
    14341 tgccgccgtc ggtgtcgccg gagatgccgc cggctctttc gttggcgttg attacaaggt
    14401 ccttagagct tacatttgta atatagctcg ttgtcttgat attgcccgca agaccgccta
    14461 tgttgttgcc cgtgccccta atcacattca tgccgggcag accgattacc gagctgtcct
    14521 taatgctgcc gtttgactca atctgacctg cgatgccgcc gacctgagcc ttggcgacga
    14581 tattaacgcc ctcaacggtg acattttcga ccttgcctct gagcaaacct acaacagcgc
    14641 cgacatattc ggatgtggag ttaagagata catttctgag agtaaggttc ttaattaccg
    14701 cgttggggtt ggtggagccg aacagaccgc ggtcgtatgt ggcgggctga atcttaaagt
    14761 tgctgattga atggttgttg ccgtcaaaga taccgttgta tctgcggctg tctgtgccga
    14821 tgggtatcca ttccttgttt cttatgttga tgtcggctat gagctttgcg ttagcgttca
    14881 catttccgtc attgacgaaa ttcctgaacc actcaagctg gaaggtgttc ttaatctcat
    14941 agtagccgtt tacaagagcg ggaggctctg cgttcgcgat aacggggaag ccgtttacat
    15001 agtcgtcatt gtactgcgcc caaaggtcgg tgatgtcgtt gagcatctcg gtcaggctgc
    15061 cgtttctgat atccgcaagg ctgacttcgg taatgcccga aacatcgccg ctggctccgg
    15121 cggaagctcc gcctgtgcgc gggaagccgt tggctctcgc aaagttgttg acaacattgc
    15181 ccgagctctt atatccaagg aagctgccca cataaatctt agtattgttg tttattgagg
    15241 catcggcgta gttttcggca attgcatggt ttgcattgtc catcttgccg ataaagccgc
    15301 ctatcattct gccgacgccg gtaatcgtta cattggatac atagttgtac tgtatcgtat
    15361 tggctgtgct ccatcctaca acaccgataa ggccgcccac gcagaactcg ttgcccgtgc
    15421 cggtgttggt ggcgttgatg gtaacattga taacgccgac tctgcttatg aaggagccgt
    15481 ctttaaggga gtatgaaacg ccgcctatgt accttgtgcc tgtaagggat gaatccttga
    15541 ttatggtatc ggtgatggtt gccttattgt tgatgtagcc gatgatgccg cctgttctct
    15601 cgttatttga aacgcaggtg aagttctcaa caaggctgtt ggtgactgac acggggttgc
    15661 cgttggatgt gccagatatg ccgcccagct cggcgccgcc tgtaattctt acatttctga
    15721 cggtagcctt atccactgtg gaagcgttgc ggacgctgaa tataacgccg ccagcgtagc
    15781 ggttgacgga gatgtttaca ttctcaacaa gagcctcgct gattcttacc gtaccgccgt
    15841 cggagtcgcc gaagacgccg cccgcttttt cattagcggt aaagtcaaca ttcttgacaa
    15901 ttacattgtt gacgggcatg gttgccttga tgttgccggc tacgccgccc acaaaggagc
    15961 ctgtgccctt aatcgtcttt gcgcccgtct cgccgatgac ggtaatattg ttaagctggg
    16021 tcgatgcctc ggactgacct ataacgccgc ccacatagga cgcgccgtta atgttcatgc
    16081 cctcaacggt gatgttggaa accgtgcctc tcaaaagacc tacaagaccg ccgacatagc
    16141 tgccgttggt gtttatgtag acattcttaa gttttaagtt tctcagaacc gctgtgggag
    16201 ccgtggagcc gaaaagaccc ttgtcgttgc ctgtctttga aatataaagg ttggagattg
    16261 taaagttctg accgtcgaat gtgccggcat agcgcacatt gttgttgccg atggtgtcca
    16321 tctttttatt gttgagattg atgtcggcaa caagctttgc ggaggcgctg gtattgcccg
    16381 cgttgacatg tttctggaac cactcaagct ggaaagcgga gccgataagg aagtagccgc
    16441 ttgcgtcctt tgagggagcg acggcaagat tcccgtcaat cacgggatat ccgtcgtttc
    16501 ctgttttcca gatgtttgta aggttgttaa ggtagctgag aagcgcgccg tttgttgcct
    16561 cagcggcggt gacgcctctg atgttggtaa ccgtagagga ggaaacctgc tcgccgatgg
    16621 cattctgacc gcttgcggtg acatagaata aatcgttcag ctcagcgttg gcgacatagc
    16681 ccaccagagt gcctgccctt gtgggagcgg ccctgaggtt taatgtcgcg gcgctgtagt
    16741 tttcggaaat gaaatgtctt gcggcctcga tttttccaat aaagccgccg ctcattctgc
    16801 cggcgctgct gacggttgtg ttgtaaacag cgttgtactg aatggtgttt gccacatcgc
    16861 ttgttacaac gccgataagg ccgccgattt caacatcggt ggtcgcgctt gtctgagtgg
    16921 aggtgatggt ggagtctaaa accatgcagc gctctataaa gctgccgttc ttgacagcaa
    16981 aagcaagacc gcccatgtac cttgtgcctg tgacattgac gcccttgaca attgtgtcat
    17041 acatgtcgaa tctgtagtcg gcgtgaccta tggcgccggc gattctcgaa ttcgaagagg
    17101 tggatgagat attaagattc tcaactcggc agtttctgaa ctgtacattg tttgccttta
    17161 tgtagcctac aagtccgccg gtgttgtttg tgccgacgat ggttttcatt gatgatgtgc
    17221 ccgtgacgga gcagtcggtg ataaccgccg cgccctctac ctgaccgata agaccgccgg
    17281 tctgagccct gccggcagaa atgtttatgc cggtaacatg gcagttgctg attgtgccgc
    17341 ccacagcaac gcccaaaaga ccgccggagt agtcacgggt gcccgcgatt gtaatgttgt
    17401 tgacattaac attcttaata acgcccgagg tggtgatttt tccgaaaagg cctcggtcaa
    17461 cagaggtgga attgacatag acatttgata tggtaaagcc ctgaccgtca aatgtacccg
    17521 cgtactggcg gtcatggttg cctatggg
//
LOCUS       DAONVQ010000024        16406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2617_length_16406_cov_4.720629, whole genome shotgun sequence.
ACCESSION   DAONVQ010000024 DAONVQ010000000
VERSION     DAONVQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16406
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2617_length_16406_cov_4.720629"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /locus_tag="PLD98_02760"
     CDS             complement(<1..1743)
                     /locus_tag="PLD98_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009167438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQA05204.1"
                     /translation="MTREEARKRAKELLEKMTVEEKISQLVYDSRSVERLGIPSYNWW
                     NEALHGVARAGLATVFPQAIGMAASFDKKLIYKVATAIADEARAKYNAAQEEGDHGIY
                     KGLTFWSPNINIFRDPRWGRGQETYGEDPYLTAAMGTEFVKGLQGDGEFMKAAACAKH
                     YAVHSGPEKGRHGFDAQVDDRDLFDTYLPAFERLVKDAKVEAVMGAYNRVNGEPACAS
                     KRLLIDILRGDWGFEGHVVSDCGAIADFHKNHHVTSSADESAALALKAGCDLNCGCVY
                     ASGNVLLALEKGLITEEDIDRSVLRLLTTRFLLGMFEEKQPYIDVPLEIVDCPEHREL
                     NLKMARESIVLLKNDGILPLDPAKLKSVAVIGPVAQSYSVLEGNYCGKAGEYTTLADG
                     IRRMLPDARVHVVPGSELYRDRSEANCLPNDGVSQAVAYARRADVTILCVGLDPTIEG
                     EEGVEARDYADGDKRNLLLPKSQQCLVEAVCDVTDRVIVITTAGSCIDLGVANTKARA
                     ILHAWYPGARGGEAVADILFGRVSPSARLPVTFYYDNNNIPDITDYRMENRTYRYSKE
                     EPLYPFGYGLSYTTF"
     gene            complement(1749..3071)
                     /gene="xylA"
                     /locus_tag="PLD98_02765"
     CDS             complement(1749..3071)
                     /gene="xylA"
                     /locus_tag="PLD98_02765"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HQA05205.1"
                     /translation="MSEIFKNVPKVRYEGQNSTNPYAFKFYDAERIIMGKPMKEHLRF
                     AMAWWHTLCFTGSDMFGSPTMDKSFGAECGTMEHAYKKVDAGFELMDKLGIEYFCFHD
                     ADIVPQADTIAETNRRLDEITDYIKEKMEKHNKKLLWGTANLFSSPIYMNGAGSTNSV
                     DVYCRAAGQIKKALELTVKIGGLGFVFWGGREGYESLWNTDMKFEQENIASLMRMAVD
                     YGRRIGFRGDFYIEPKAKEPMKHQYDFDVATSIAFLRRYGLDKDFKINIEANHATLAG
                     HTFQHELRVAAINGMLGSIDANQGDLLLGWDTDEFPYNVYETTACMYEVLKAGGFKNG
                     GLNFDAKTRRPSNTYEDLFISFILGMDSFALGLINAARIIEDGRIDAFVKERYSSFES
                     ELGRKIREGKATLEELAEYSEKAVSVTVPDSGRQEYLLSVINQLMLKG"
     gene            3313..3606
                     /locus_tag="PLD98_02770"
     CDS             3313..3606
                     /locus_tag="PLD98_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQA05206.1"
                     /translation="MAELGAKEFKLLSRLMENPERVFTKRQLFLAVWDDEYYYDDNPL
                     RDIASSQYGLIDKVTFFCGKVRTYNDVYQRLYDIDLSEAYKELSGHSAKRRVP"
     gene            complement(4063..4500)
                     /locus_tag="PLD98_02775"
     CDS             complement(4063..4500)
                     /locus_tag="PLD98_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05207.1"
                     /translation="MDRKMEIVRILLKIIMWFLIAEFSCNFVMQTISYSFYKNAKKME
                     DISATPQFIRFNDSLTGYGANLDSDKGKVILFFGGSKFIAYNSVGKYSAKFDCPFISA
                     DYYGTQESGGRMNLKTMKQTAEDLHDWAREHYPNSEIVIIGHS"
     gene            complement(4552..5547)
                     /locus_tag="PLD98_02780"
     CDS             complement(4552..5547)
                     /locus_tag="PLD98_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C
                     domain-containing protein"
                     /protein_id="HQA05208.1"
                     /translation="MKLKKALKFLLIIPAVFLTAFLALCINGTCPYKNHVADESLSAW
                     MSKIPDDTLIKEIAIPGSHDAGTIGMPWTFRTQNYSIGDQLTFGARYFDIRVNKTEKG
                     YPIFHSSMNGVDFRDVLQEIKAFIKAHPTEVLLLDFQHFKGGSQGDVYEFLSEELDKE
                     NLILHNDTDKSDVEFISSLTLGEARGKCIVFYGGTDNDFSDWVFLRNNDECTKDNMTL
                     DSYYIGKLHRGGFYTLVNEAHPIYFEHLERNQKENKDCIFVLQSQLTDGKLVLGPWAI
                     ERGQEKKMSEYVKNLKDSPYIKSINVIMRDFLIPEKCQEIIDLNIAKGLMDAPQS"
     gene            complement(5681..6583)
                     /locus_tag="PLD98_02785"
     CDS             complement(5681..6583)
                     /locus_tag="PLD98_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQA05209.1"
                     /translation="MGFSRTDFMFPSHGDKCSAWLYLPDNGSDRHPLVVMAHGFGGMK
                     NCGLEPYAEYFADNGLAVLLFDYRGFGDSEGTPRQLISHRRHIEDYHAAVAFARRQKN
                     INPDKIALWGSSYSGGHAIVAASQDPKIAAIVVQVPFVSGIASSAPIVKQNGAKYFFK
                     AAASTFKDLWRAATAREPYMVPVYGAPDEFAVLNTRDSKTGYESLIPQGLEVVNAAPA
                     RVFLTMVLYRPLSAASKVNCPALIIAGEKDSLIPLESVKKAASKMRQAELITLPLDHF
                     EPYLGEPFKKVSQKQLDFLKAHLF"
     gene            6913..7230
                     /locus_tag="PLD98_02790"
     CDS             6913..7230
                     /locus_tag="PLD98_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05210.1"
                     /translation="MKTDSGTVILKQRGSREDFRERIKRPAEEVRVTAHLACMKKLPL
                     ETILEWVNDEDYNVRHGAAFACRNSNVPIEKIQRLLEDEHPSARRTASKLYFELLNRM
                     EEE"
     gene            complement(7297..9222)
                     /locus_tag="PLD98_02795"
     CDS             complement(7297..9222)
                     /locus_tag="PLD98_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HQA05211.1"
                     /translation="MKDRIFTSMLLENDPDSGAESGIALSLKFIGSNDTKPQGIKERL
                     TKINYIIGKDRSKWRRNLPTFDGVKYTGVWDGVDLEIEANKEGLKLYWKVSPHADPSK
                     IQLEYMGANRLSVTETGELKIEHDLGESFDKAPVAYQIINGEKTEVPCEYRLDSDLAR
                     FSFALGEYDPNFELIIDPLMPYGSYLGGSEQDAGMSICVDEAGSAYVAGYTISEDFPI
                     IPGAYQIAPEQTDWDSGFVSKFTPDGSDLIFSTYLSADRSVIIYAIDIDDDLCVYVTG
                     DTNSLDFPTTDNAFRPDITGNEDGFLTKFAADGSDLVYSTYLGSFRCFPRALKVSPDG
                     FAYVGGFATTDYPTTPGAYQNWVATNYSGFVTKFSQDGSAQVFSTLFSGSNYTRIQGI
                     AVDDSGCVFAAGYTQDVNLPTTDGAFERELQGSQCAFAVKFNDDGTNIICSTYFGGSN
                     LTWANGICADAEGNAYLTGTVFTGGLPVTPNAPQPVPLNDRCGFAAKLNADFSDLIYS
                     TYLTCMGTVDGMDIKLLSGNRACVACWGDEEADTFPSTPMITPVDNGRSIIVILTDDG
                     SDFYASQTIAPNIGVSLAIGNDSSIYITGWTNAEQFIPTAGAFQTEYGGGPNDAYVMR
                     VKYIDVANSSVWIKRIG"
     gene            complement(9345..10094)
                     /locus_tag="PLD98_02800"
     CDS             complement(9345..10094)
                     /locus_tag="PLD98_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05212.1"
                     /translation="MASIALQLIAESINIDINSQESIVFDVVRYASDSVSYNPATGEI
                     TLFGIGSYTAHWWVATQSVSRSGGASLALVSNQGSEQIGNSPIKTGEVYGFGVFEVTE
                     DEPVTISLKNITDGTMFLSTDEPVKASLILNYDVLPAFGFFFTPYRTSHWAIESGNNF
                     VFYENGPSVNVEHDPVSSEVTVLLSGTYEISYVFSFIEPANILISLLKNNVPVHTIPL
                     ANFTGALSDTIVLPLSAGDVLKWEFEFLEED"
     gene            complement(10433..11320)
                     /locus_tag="PLD98_02805"
     CDS             complement(10433..11320)
                     /locus_tag="PLD98_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQA05213.1"
                     /translation="MKNCVLCPRECHADRLSGKSGYCKAPAELLVARAALHFWEEPCI
                     SGKEGSGTVFFSGCALGCVYCQNRSISKGLEGKAITIERLAEIFLELQAKGANNINLV
                     TPSHYVPHIIEALNISRRNGLTLPIVYNCGGYEKTETLKMLEGYVDIYLPDLKYISDE
                     PAIKYSNCKGYFTAAKAAIEEMVRQAGTAVFDERGIMRKGVIVRHLTLPGYLEDSKRI
                     VKYLYESYRNKIFISIMNQYTPMTKSTEYPELNRKISYEEYEELVNFAIEIGVENGFI
                     QEGEPASESFIPEFNGEGV"
     gene            complement(11457..11936)
                     /locus_tag="PLD98_02810"
     CDS             complement(11457..11936)
                     /locus_tag="PLD98_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQA05214.1"
                     /translation="MKPEFTFRYATREDIGLVLQYIKELAEYEKMSDEVIADEKTLEH
                     WIFDKQLAEVFFALEDGKEVGFALYFHNFSTFVGRAGIYLEDIYVKPEYRGKGYGKAM
                     LKKLASIAVERGCRRLEWMCLDWNKPSIEFYKSLGAIPMSEWTLYRLTGDALTSLAE"
     gene            complement(11947..12693)
                     /locus_tag="PLD98_02815"
     CDS             complement(11947..12693)
                     /locus_tag="PLD98_02815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQA05215.1"
                     /translation="MTKSKSNQKGLYVAAVALILIFQQFASKVGNLVAGCFDYKAIDE
                     YGIFAHVSVHHIVQLLIALLAISILAKKLKIDFGFGLGNKKTGTKYVLWFTAAVFGFT
                     AITYAVRYFGNQIIQYDYPLNAKNVLGTLGFQLLLSGPSEEVLFRALPIGVLAFLNES
                     NKERRFTKLNISLEVIIAAVLFSIAHIKWTVSPFSISMNILQLIYCFVLGIADGKAYQ
                     ESGSVIYPMMMHSISNVITVGLGYILTIIV"
     gene            complement(12981..13202)
                     /locus_tag="PLD98_02820"
     CDS             complement(12981..13202)
                     /locus_tag="PLD98_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05216.1"
                     /translation="MFNYIAESDVTLSPGFNRDTPKPFAVSGSKSFIYPVTAGTRLAM
                     LVYVRSEEINAEAAGTTTNFSGGVTILFD"
     gene            complement(13172..13903)
                     /locus_tag="PLD98_02825"
     CDS             complement(13172..13903)
                     /locus_tag="PLD98_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05217.1"
                     /translation="MSNYLFVEYTLNDPILSSTAIPFNNIKSQNGISYNDLTGENRFD
                     EPGTYQVNWWVTTQSSTAPNANFVLYHSDNGQTYVDQSCSPAKTGQTSGTAVFQVTDV
                     PHTAYLRNESGQTFYFAQSVPVKASLTAFGIPSQGIALVPFGSIYVDSMVDSEEAGFY
                     MGNPAFGGQAYSYYFETFPLSIDMEEFMEMQAFSVPKDGVLTGISGAGMMFSGEMGFD
                     TYYLHAALYACPPAAMCSTILPNPM"
     gene            complement(14431..15300)
                     /locus_tag="PLD98_02830"
     CDS             complement(14431..15300)
                     /locus_tag="PLD98_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQA05218.1"
                     /translation="MDSLSYLNKAMEYIEEHLTEDIDFSEISKIACCSEYHFKRMFSF
                     LSGIGLSEYIRRRRLTLAALDLRDESLRIIDIAVKYGYDSDDSFSRAFRAMHGVLPSE
                     VRKSNIQLKAYPRMTFQLSINGGCEMKYRIVEKGPFKIVGFKKRVPIIFNGVNPEIAK
                     MAELLTPEIIEKLKALSNVEPTGIINASANFSEGRMEEKGELDHYIGAATTSDETAEF
                     DVLNVEAGTWAVFEAVGPFPETLQNVWGRIYSEWFPSSGYEAVPGPEILWNESQDTKE
                     PNYRSEIWIPVRK"
     gene            complement(15580..15885)
                     /locus_tag="PLD98_02835"
     CDS             complement(15580..15885)
                     /locus_tag="PLD98_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQA05219.1"
                     /translation="MKVFNKSREALIAVCTLAGAYILISALVIFNVEPDSFNTFFDAV
                     YWATVSLTTVGYGDIYPVSTIGRIVTMFSSVFGIAIVALPAGIITAGYMNEIKDSRD"
     gene            complement(15882..16229)
                     /locus_tag="PLD98_02840"
     CDS             complement(15882..16229)
                     /locus_tag="PLD98_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HQA05220.1"
                     /translation="MIAAIIISIIPLAFKKDTLLFQIADKVVLVIFICDYILRLITAD
                     YKYNKMSVSSFIKYPFSFMAIIDLVAILPSVTILNDGLKLLRILRMIRASRVLRVFKV
                     LRYSKSMSIIALL"
BASE COUNT         4320 a         3907 c         3654 g         4525 t
ORIGIN      
        1 aaatgtggtg tagcttagtc cgtatccgaa aggataaaga ggctcttctt tcgaatagcg
       61 gtaggtgcgg ttttccatgc ggtaatcggt gatgtcaggt atattgttat tgtcataata
      121 gaaggtaacg ggcagacggg cggaggggct gactcgtccg aacagtatat cggccaccgc
      181 ttcgccgcct cgggcgcccg gataccaagc gtggaggatt gccctggcct ttgtgttggc
      241 gacaccgagg tcaatgcagc tgcccgccgt ggtaatgaca ataacacggt cggtcacatc
      301 gcagaccgct tccacaaggc attgctgact cttggggagc agcagattcc tcttgtcgcc
      361 gtcggcgtaa tcccttgcct ctacgccctc ctcaccctcg atggtggggt caagtcctac
      421 gcacaaaatg gtgacatccg ctctgcgggc gtaggccaca gcctgagata cgccgtcatt
      481 cggcagacag tttgcctcgc ttctgtcacg gtaaagctcg ctgccgggaa ctacatgaac
      541 ccttgcgtcc ggcagcatac ggcggatacc gtcggcaagg gtcgtatact cgcccgcctt
      601 gccgcaatag ttgccctcaa gcacggaata agactgggcg acggggccta ttaccgcaac
      661 ggattttagt ttggcggggt ctaaaggcaa aatgccgtca tttttaagaa gcactattga
      721 ctcccgagcc atttttagat tcagctctct atgctcgggg cagtccacaa tttcaagggg
      781 gacatctatg tagggctgtt tttcctcaaa cattccaagc aggaatctgg tggtcaaaag
      841 tctgagaacg gagcggtcaa tatcctcctc ggttataagt cctttttcca acgccagcag
      901 aacattgccc gaggcgtaaa cgcagccgca gtttaaatcg cagcccgctt taagcgccaa
      961 agcggcggat tcatcagcgg aggaggtaac atggtggttt ttatgaaaat ccgcgatggc
     1021 gccgcagtct gaaaccacat gaccttcaaa accccaatcg cctctgagaa tatcaatcag
     1081 cagccgcttg cttgcgcagg cgggctcgcc gtttacacgg ttgtaagcgc ccatgacggc
     1141 ttccaccttg gcgtccttta caagacgctc aaaggcgggc aggtatgtat caaaaaggtc
     1201 acggtcgtca acctgagcgt caaaaccgtg acgccccttt tcggggccgc tgtggacggc
     1261 gtagtgcttt gcgcaggcgg cggctttcat aaattcaccg tcgccctgaa gtcctttaac
     1321 gaactcggtg cccatagcgg cggtaaggta cgggtcctcg ccgtaggtct cttgtccgcg
     1381 tccccagcgg gggtcacgga aaatattgat gttgggactc cagaaggtaa gacctttgta
     1441 tatgccgtgg tccccctcct cctgcgccgc gttgtatttt gccctcgctt catcggcaat
     1501 agcggtggcg actttgtaaa tgagcttctt atcaaaggac gccgccatgc cgatagcctg
     1561 ggggaaaacc gtggcaagcc ccgctctggc gacgccgtgc aaagcctcgt tccaccagtt
     1621 gtatgaaggt atgccaagcc gctccactga gcgggagtca tatacaagct gagatatttt
     1681 ctcctctacg gtcatttttt cgaggagctc ttttgctctt tttctcgctt cttccctcgt
     1741 catagccctt acccctttaa cataagttga ttgataacgc tcaaaagata ctcctgtctg
     1801 ccggaatcgg gaacagtgac gcttaccgcc ttttcggaat actccgccag ctcctcaaga
     1861 gtggcctttc cctctcgaat ctttctgccc agctcagact caaatgagct ataacgctct
     1921 tttacaaagg cgtcgatacg cccgtcctct ataatgcggg cggcgttaat tagacccaaa
     1981 gcaaagctgt ccatgcccag aataaaggaa atgaacaaat cctcataagt attgctcggg
     2041 cggcgggttt tggcgtcgaa attaaggccg ccgtttttaa atccgcccgc ctttaaaacc
     2101 tcgtacatgc aggcagtggt ttcgtaaaca ttataaggaa attcgtccgt atcccagccg
     2161 agaagcaaat cgccttgatt agcgtctatg gagcccagca ttccgtttat ggcggcgaca
     2221 cgcagctcat gctggaatgt atgccccgca agtgtggcgt ggttagcctc gatattgatt
     2281 ttgaaatcct tgtcaagacc gtaccgccga agaaaagcga ttgaagtcgc cacatcaaaa
     2341 tcatactgat gcttcatagg ctcttttgct tttggctcaa tataaaaatc gcctctgaaa
     2401 ccaatgcgcc tgccgtaatc gaccgccatg cgcatcaaag aggcgatgtt ctcttgctca
     2461 aatttcatgt cggtattcca gagcgattca tagccttctc tgccgcccca gaaaacgaag
     2521 ccgagcccgc cgattttcac ggtgagctca agagcctttt ttatctgtcc cgcggcgcgg
     2581 cagtagacat caacgctgtt tgtgctgccc gctccgttca tgtagatggg actgctgaaa
     2641 agattcgcgg tgccccagag cagcttttta ttgtgttttt ccatcttttc ttttatgtag
     2701 tcggtgattt cgtcaaggcg gcggttcgtc tcggcgatag tatccgcctg cggaactata
     2761 tccgcgtcgt ggaagcagaa atactcaatc cccagcttat ccataagctc aaagcccgcg
     2821 tccacctttt tgtatgcgtg ttccatagtg ccgcattcgg cgccgaaact cttgtccatt
     2881 gtcggcgagc cgaacatatc ggagccggta aagcacagag tgtgccacca agccatggca
     2941 aagcgcagat gctccttcat gggcttgccc attatgattc gctcagcgtc ataaaactta
     3001 aaggcatagg ggttggtgct gttttgccct tcgtaccgta ctttcggcac attcttaaat
     3061 atctcgctca aactcatgcc cccattcgcg ttggtatatt attccttttc cattatagtt
     3121 tttttatttc tatataacaa gaaaaaactt cattttgttt aagtaaatac ctttttgcgg
     3181 gcaaatcgtt tttagcggga tattttatat attgcaattt agaaaattgc gaggagggcg
     3241 gcgcatgaac attcttattg cgggaaactc actattgaca aagaaaaatg cggtctgtat
     3301 atcgatgggc agatagcgga gctcggggca aaggaattca aactgctttc acggcttatg
     3361 gaaaacccag agcgggtgtt taccaaacgg cagctctttc tggctgtttg ggacgatgaa
     3421 tactattatg acgacaaccc tctgcgggat atagcctcct cgcagtatgg gcttatcgac
     3481 aaagtcacct tcttctgcgg taaagtccgc acctataacg atgtttacca gcggctttat
     3541 gacattgatt tgagtgaggc ttataaagag ctcagcgggc actctgcaaa acgcagggtg
     3601 ccctaagttt tgacttatta ttaaattgag aagtcaacgc ataaagccgg tatgtcggct
     3661 aaacaatcag gaccaatatt cactttgctg tttctgtttt tggctttaaa tctcacctat
     3721 cgcctgtttt atataatcaa tcaccttatc cgagctgaaa taatcctcat gggcaatacc
     3781 gtcgaaaatc tttacttcgg gatttttaaa gcagcgggca agtttttcct gaagtgaaac
     3841 gctcaggact ttatcgcccg ttgagccgat aatgcaaacg ggacactgaa cattttgggc
     3901 atattccgag gcgcggatat tgtttgaaat aaaaattttc agcggacccc ggaatacggg
     3961 cgtgattttg ttatttaagt ccgaaaggtc tctgtatccc gcggctaaaa tcagagcgcc
     4021 gcactccctg acgcttgcaa ggaaagccgc tattcccgct cattagctgt gcccgataat
     4081 tacaatctcg ctgttggggt aatgctctct tgcccagtca tgcaaatcct cagcggtttg
     4141 cttcatggtt ttgagattca ttctgccgcc gctttcctga gtgccgtaat aatccgccga
     4201 aataaatggg caatcgaact ttgcggagta tttcccgacg gaattatagg ctataaactt
     4261 tgagccgccg aaaaacaata ttaccttacc tttatccgaa tcaaggttag ctccatagcc
     4321 cgtcaaagaa tcattgaacc gaatgaactg cggagtggcg gagatatcct ccatcttttt
     4381 tgcgtttttg taaaacgaat agctgattgt ctgcattaca aaattgcatg aaaactccgc
     4441 aatcaaaaac cacattatga ttttcagcag gattctgact atttccattt ttctgtccat
     4501 ctcgtgtaac ctttaagatg cgtttttgga ggtaaagcca tagaaagttt tttaactctg
     4561 aggagcatcc attaatccct tagcgatatt aaggtctata atttcctgac acttttcggg
     4621 gatgaggaag tcgcgcatta taacattaat gctctttata tacgggctgt cctttaaatt
     4681 ctttacatac tcgctcattt tcttttcctg acctctttca atagcccagg gaccgagaac
     4741 cagttttccg tcggtgagct gggactggag cacgaaaatg cagtccttgt tttccttttg
     4801 attcctttca agatgctcaa aatatatggg gtgcgcctca ttcacaagcg tatagaagcc
     4861 gcccctgtgg agcttgccta tgtagtagga atccagagtc atattgtctt tggtgcactc
     4921 gtcgttgttc cttaaaaata cccagtcgga aaaatcatta tccgtgccgc cgtagaaaac
     4981 aatgcacttg ccccttgcct cgcctaaggt aagagagctt ataaactcca catcgctttt
     5041 gtccgtatcg ttatgaagaa tcaggttctc cttgtccagc tcctcggaca aaaactcata
     5101 cacatcgccc tgactgccgc ccttaaaatg ctggaagtcg agaagcagaa cctccgtggg
     5161 atgcgctttt atgaacgctt taatctcctg caaaacatct ctgaaatcaa cgccgttcat
     5221 ggaggagtgg aatatcgggt agcctttttc cgtcttgttt acccttatgt caaaatacct
     5281 tgccccgaag gtaagctggt cgcctatgga atagttctgc gtgcggaagg tccatggcat
     5341 gccgattgta cccgcgtcat ggctgccggg gattgcgatt tctttaatta gcgtgtcgtc
     5401 ggggattttg ctcatccaag cggacaaaga ctcatcggca acatggtttt tatacggaca
     5461 ggtgccgttt atgcataaag ccagaaacgc cgtcaaaaac accgcgggga tgataagcag
     5521 gaactttaat gctttcttta acttcatact ttacctccga aaacaggctt tatgtttaag
     5581 cctttttgtg ggtttgccgc ctgtggtccg aaatattagc tctgttccat tatacattat
     5641 ccttgatagc ttatagaaca tgagctaagt tttaacgggc ttagaataaa tgggctttta
     5701 aaaagtcgag ctgtttttgg gagacctttt tgaagggttc gccgaggtat ggctcgaaat
     5761 ggtcgagggg cagggttatt agctctgcct gtctcatttt tgatgccgcc ttttttacgg
     5821 actctaacgg gataagagaa tctttttcgc ccgctataat cagcgccggg cagtttactt
     5881 tgcttgccgc gcttaagggt cggtacaaaa ccatggtaag gaaaactcgt gcgggggcgg
     5941 cgtttaccac ttcgagaccc tgagggataa gtgattcata gcccgttttg gagtcgcggg
     6001 tgttaagtac ggcgaattca tcgggcgctc cgtagacagg caccatgtaa ggctcccttg
     6061 cggtggccgc ccgccataaa tccttaaatg ttgacgcggc ggctttgaaa aagtattttg
     6121 ccccgttctg ctttactatg ggcgcggagc tggctatgcc cgaaacaaat ggaacctgaa
     6181 ccacaatagc ggcgattttc gggtcctgag acgccgcaac aatagcgtga ccgccgctgt
     6241 atgagctgcc ccacagagca attttgtcgg gatttatatt cttttgcctg cgcgcgaatg
     6301 ctacggcggc atgatagtcc tcaatatgcc tgcggtggga aataagctgc ctcggagtgc
     6361 cttcgctgtc cccaaaaccc ctgtaatcaa agagcagaac cgccagaccg ttgtcggcaa
     6421 aatattcggc atagggctcg aggccgcagt ttttcattcc cccaaagccg tgggccataa
     6481 caaccagcgg gtgcctatcc gagccgttgt cgggcagata cagccaagcc gaacacttgt
     6541 cgccgtgact aggaaacata aaatcagttc ttgaaaaacc catagtatgc tctcctttct
     6601 gacttgagcc gcaaagattt tcccaattat gccatttcca ttataagaca aaaaagcctt
     6661 gtataacaag gctttttata atattcacct tctgcgctat tgctcctttg cggttttaaa
     6721 ttccgggcaa tttatgggac acagaaaatg ctgtgatact ggcagtaagt ttgcctaagg
     6781 gggttttggg cttggatatg gagcaaaaga gctggggatt tgtactcaag tttgtgatta
     6841 tgagcaagga agagacggag tttcataaaa acaagatgaa aaagctgggt caatcgctta
     6901 aggctgagca gattgaagac agactcagga acggtgattt taaaacagcg aggttcccgc
     6961 gaagatttta gagaaaggat taagagaccc gccgaggaag taagggtaac ggcacatctt
     7021 gcctgcatga agaagctccc ccttgaaact attttagagt gggtgaatga cgaggattac
     7081 aatgtaagac acggcgcggc ttttgcatgc cggaacagca atgtaccgat agaaaaaatt
     7141 cagcggcttc ttgaggacga acacccgtcc gcaaggcgga cggcttctaa gctatacttt
     7201 gaattgctaa accgcatgga ggaagaataa aaaagattac agaaaaaagc aaacagaggc
     7261 agagagcctt tgtttgcttt ttgcgcctga attctcttac ccgattcttt ttatccatac
     7321 cgacgagttt gcgacatcta tatattttac gcgcattaca taagcgtcgt ttggaccgcc
     7381 gccgtattcg gtttggaatg ctcccgccgt cgggataaac tgctcggcat ttgtccatcc
     7441 ggttatatag atgctgctgt catttccgat agccagagag acaccaatat taggggcgat
     7501 tgtctgagaa gcgtaaaaat cgcttccgtc gtccgtcaga ataacaataa ttgacctgcc
     7561 gttatccaca ggggttatca ttggcgttga tgggaaggtg tcggcttctt catcgcccca
     7621 gcaggcaacg catgcccgat tgcctgacag cagctttata tccatcccgt caacggttcc
     7681 catgcaggtt agataagtgg aatagataag gtcggaaaaa tcggcgttga gtttagcggc
     7741 aaagccgcat ctgtcattca agggaacggg ctgaggagca ttgggggtta cgggcaagcc
     7801 tcccgtaaat acggttcccg taagataggc atttccctct gcgtctgcgc aaataccgtt
     7861 ggcccaagtc aaattgcttc cgccgaaata agtggagcag attatgtttg tgccgtcatc
     7921 gttaaacttt acggcaaaag cgcattgcga gccctgcaat tctctttcaa acgctccgtc
     7981 tgtagtaggc aaattaacat cctgagtgta gcccgcggca aaaacgcagc cgctgtcatc
     8041 caccgctatt ccctgtattc gagtatagtt cgaaccgctg aacaaggtgg aaaacacctg
     8101 agcggagccg tcctgcgaaa acttggttac aaagcccgag taatttgtcg ctacccagtt
     8161 ctgatacgct cccggtgttg tgggatagtc ggtagtggca aagccgccca cataggcaaa
     8221 gccgtcaggg gatactttga gcgcgcgggg gaaacatctg aaactgccca aataggtgga
     8281 atacactaaa tcggagccgt cggcagcaaa tttggttaaa aagccgtctt catttcctgt
     8341 aatgtcagga cgaaacgcat tgtcggtcgt ggggaaatcc aatgaatttg tatctccggt
     8401 tacatacacg cacaaatcat catcaatatc tatcgcgtaa ataattacgg acctgtctgc
     8461 cgataaatag gttgaaaaga ttaaatcgct gccgtcggga gtaaatttgg aaacaaagcc
     8521 tgagtcccag tcggtctgct cgggcgcaat ttggtacgcg ccggggatta tcggaaaatc
     8581 ttccgaaatg gtatatcctg ctacatatgc gcttccagct tcgtcaacac agatgctcat
     8641 acccgcatcc tgctcgcttc cgccaagata ggagccgtag ggcatcagcg ggtcgatgat
     8701 tagctcgaaa ttcgggtcat actcgccgag agcaaatgag aatcgggcta agtcggagtc
     8761 aaggcggtat tcgcagggaa cttccgtctt ttcgccgttt atgatttgat aggcaacggg
     8821 agctttgtca aaggattctc ccaagtcgtg ctctatttta agctcgcccg tttccgtaac
     8881 ggacaagcgg ttcgctccca tatattcgag ctgtattttg gaggggtccg cgtggggaga
     8941 tactttccag taaagcttta agccttcttt gttggcttca atctccaaat ccaccccgtc
     9001 ccagacgccc gtatatttca cgccgtcgaa agttggaagg tttctgcgcc attttgaacg
     9061 gtcttttccg ataatgtaat tgatttttgt gagcctctct ttaattcctt gaggcttagt
     9121 atcgtttgag cctataaatt tcaggctcag cgcaatgcct gactctgccc cgctgtcggg
     9181 gtcgttttca agcagcatag aagtaaaaat tcggtctttc ataaagaaat attccgtgga
     9241 ttttgctttg catttatagt aaacgccatc atggcttccg acatactttt caaagtatat
     9301 cgggcggcgg tataaggttt gttgctttat cataaggacg cacctcagtc ttcctcaagg
     9361 aattcaaact cccacttgag cacatcgccg gctgagaggg gcagaacaat cgtatccgac
     9421 aaagcgcctg tgaagtttgc caggggaatt gtatgaacag ggacattgtt tttaagaagg
     9481 ctaatcaaaa tattagcagg ctcaataaat gagaatacat aggaaatctc gtaagttccg
     9541 ctgagcaaaa cggtaacttc gcttgaaacc gggtcgtgct ctacattgac agaaggaccg
     9601 ttttcataga agacaaaatt gtttcccgac tcaatcgccc agtgtgatgt tctgtaaggc
     9661 gtgaaaaaga agccgaacgc gggcaataca tcgtaattaa gaatcagcga tgccttaacg
     9721 ggctcgtcgg tagacagaaa catggtgccg tcggttatat ttttgagtga aattgttaca
     9781 ggttcgtctt ccgtaacttc aaatactccg aaaccgtaaa cttccccggt ttttatcggc
     9841 gagtttccga tttgctctga accctggttt gacaccaaag ccaagctggc gccgccgctt
     9901 cgtgacaccg attgggtggc gacccaccag tgggcggtat agctgccgat gccgaaaaga
     9961 gtaatctctc ccgttgcggg attgtagctt acggaatcgc ttgcgtaccg cactacatca
    10021 aacacaatgc tttcctgaga attaatatct atgtttatcg attcagcgat gagctgcaaa
    10081 gcgatgcttg ccattataaa agccccttac attaaaattt aattaaacac agtcaagcca
    10141 ttatatgtcc gattaaaatt cactgtgaca tgcctttttg gtcggagcac cttaaccttt
    10201 atttcaggaa aaaagaaaag cgcctatcgt tggatagacg ctaaaaacta atattttaac
    10261 cttaattttc cctttaaggc cattataaag catgcaaatg attttgcaaa caatattcgt
    10321 tccaccacaa aaaatcagct atgttcaggt tcgccactat cttagcaacc gaacccgaaa
    10381 cacagcttat tttaacctta aagccgtctt taattgtggg agctaagctt tcctatacac
    10441 cctctccgtt aaactcgggt ataaaactct ctgaagcggg ctccccttcc tgaataaagc
    10501 cgttctccac cccgatttct atggcgaaat ttacaagctc ctcatattct tcatagctta
    10561 tctttctgtt cagctcaggg tactccgtgc tttttgtcat gggggtatat tggttcataa
    10621 tgctgatgaa tattttgttt ctgtaggatt catataggta tttaacaatt ctctttgaat
    10681 cctcaagata gcccggaagt gtcaggtgcc ttactatcac gccttttcgc attatgcccc
    10741 gctcgtcaaa gactgccgtt cccgcttgcc ttaccatttc ttcaattgcc gctttggctg
    10801 ccgtgaaata gcctttgcaa ttggagtact taattgcggg ctcgtcggat atgtatttca
    10861 aatcgggcaa atagatgtca acatagcctt caagcatttt tagagtttcc gttttctcgt
    10921 acccgccgca attatacact atggggagag taagcccatt tcttcgtgat atgttcaaag
    10981 cctctataat atggggaaca tagtggcttg gagtgacaag atttatgttg tttgctccct
    11041 ttgcctggag ctcaagaaag atttccgcaa gtctttcgat tgtaattgcc ttgccttcca
    11101 aaccttttga aatgctcctg ttttgacaat atacgcagcc gagggcgcag ccggagaaaa
    11161 acacagtgcc cgagccctct tttcccgata tgcagggctc ctcccaaaaa tgaagcgccg
    11221 ctctcgcgac caaaagctca gcgggagctt tgcagtatcc gcttttgccg cttagcctgt
    11281 ccgcgtgaca ttcccttggg caaagaacgc aatttttcat taaatttgac gccttctgta
    11341 tttcgctttg gggaattgat atgatgcttt ccgaatccat agcccctaca cccaatcata
    11401 tgtatttact gccgcatttt cttagtccgc aaagagaagg cttcagatat cgggatttac
    11461 tcggcaagtg aagtcagggc gtctcctgtg aggcggtaga gagtccattc ggacatcggg
    11521 atggcgccga gggatttata gaactcaata ctgggcttgt tccagtcaag gcacatccac
    11581 tcaaggcggc gacagccgcg ctcaacggcg attgaggcaa gctttttaag catcgctttt
    11641 ccgtagcctt ttccgcggta ctcgggcttt acatagatat cctcaaggta gatgcccgcc
    11701 ctgcccacaa aggttgaaaa gttatgaaaa taaagggcaa agcccacttc cttgccgtct
    11761 tcaagcgcaa aaaagacttc ggcgagctgt ttgtcaaaaa tccaatgctc cagagttttc
    11821 tcgtcagcga taacttcatc cgacattttt tcgtactcgg caagctcttt gatatactgc
    11881 aatacgagcc caatatcctc acgggtcgca tatctgaatg taaattcagg cttcatttat
    11941 gtcctcctat acaataatcg taagtatata tcctaaaccc accgtaataa catttgaaat
    12001 gctgtgcatc atcattgggt atataacact tccgctttcc tgatacgctt ttccgtctgc
    12061 tatgcctagc acaaaacagt atataagctg aaggatgttc atactgattg aaaatgggct
    12121 gacagtccat ttaatatgcg caattgagaa caatactgcg gcaataataa cctctaaaga
    12181 gatgttcagt tttgtaaatc ttctctcttt attgctttca tttaagaatg ccagaacccc
    12241 tatcggcaaa gcgcgaaaca aaacttcctc cgacgggcct gagagcagca gctggaatcc
    12301 caatgtacca aggacatttt tcgcgtttaa cggataatca tattgaatga tttgattgcc
    12361 gaaatatctg accgcataag taattgcggt gaatccaaac acagcggccg taaaccacag
    12421 aacatacttt gttcctgttt ttttattgcc caaaccgaaa ccaaaatcaa ttttcagctt
    12481 tttggcaagt atcgatatgg ccaataacgc tataagaagc tgcacgatgt gatgaaccga
    12541 cacatgagcg aaaatgccgt attcatctat tgccttataa tcaaaacagc ctgcgaccag
    12601 atttcccact ttgctcgcaa attgctggaa tattaatatc aaggctactg ccgctacata
    12661 caaccctttt tgattgcttt tacttttagt catcttccta tctccttggt gggattcaat
    12721 ctgcgcactc ataactgcag aaggctttag aaggtatcac ttaattatat caagtaactt
    12781 ttgtaaaaac cagaaaaata tttaaaactc acaaattccg taaatcaatg cattattatc
    12841 agcaggcagg gaaccgagat gcagcgctga aaggatattt aatcttcttt ccttagataa
    12901 agcgtccgca atttgtacct ttggaaagtg tttattaaag caaaaaataa aggctttcgc
    12961 ccttccttta tttagtgttg ttaatcgaac aaaatcgtga ctccgccgct gaagttggtg
    13021 gtggttcccg cggcttccgc gtttatttcc tcggagcgca cataaacaag cattgcaagg
    13081 cgtgtgcctg ccgtaacggg ataaatgaaa gatttgctgc cgctgacggc aaaaggcttg
    13141 ggcgtatccc gattaaaacc ggggctcagc gtcacatcgg attcggcaat atagttgaac
    13201 acattgctgc cgggggacaa gcgtaaagag cggcgtggag gtaataggta tcaaagccca
    13261 tttctccgct gaacatcatt cccgcgccgg aaatgccggt gagcacgccg tcctttggca
    13321 ctgaaaatgc ctgcatttcc ataaattctt ccatgtctat gctcaagggg aaggtttcga
    13381 aataataaga ataggcttga cctccgaagg cggggttgcc catatagaaa cccgcttctt
    13441 cgctgtctac catggaatca acataaatgc tgccgaaggg aaccaaggca atgccctgcg
    13501 acgggattcc gaacgcagtc agggacgctt taacgggtac gctttgagcg aaataaaagg
    13561 tctgaccgct ttcgtttcta aggtacgccg tatggggcac atctgtcacc tgaaagaccg
    13621 ccgtaccgct tgtttggccc gtctttgcgg gggaacagct ttggtcaaca taagtttggc
    13681 cgttgtctga gtgatacaga acgaaattag cgtttggcgc cgtggacgac tgagtggtga
    13741 cccaccagtt tacctgatag gttccgggct cgtcaaaccg gttttctccg gtaagatcat
    13801 tgtaagaaat tccgttctga gattttatat tattaaaagg tatggctgta cttgaaagaa
    13861 tagggtcgtt cagcgtatat tccacaaaca agtaattgct catatcaaga cctcattttc
    13921 acttttttac catactatgc ctgcgggtca aaggaagtga ttccgcagca gaatacacgc
    13981 agaaatcttc cggaaaacaa aaaacacccg gcaaaatcgg gtgtttaaag tgtcggcttc
    14041 ttcattaacc ttaaactact gtcatcatgg agcataaagc tcacgatgct aatgaagaaa
    14101 ctgcagacag agcttttgaa acttgtcact tccaaggcgg attataaata gttggcaatg
    14161 aaaatcatcg ccttagtggt cagaaacaaa agttattcct tgaaaacatc aaccgagatg
    14221 agctaaagaa gcgcatggct gatataagta tattacttta ggcgcagtcc actatgctta
    14281 caaaatttga tgagccgctt gtcggaaggt tgcggtctta gaatacgatt gcaccgtgga
    14341 attcaaatcc gcagtaaaat taataagata taacaagcaa ggcgctctaa acagtgcctt
    14401 gctttagttt aggttgtatt ctgattttta ttatttcctt acaggaatcc agatttcgct
    14461 tctgtaattt ggttctttgg tgtcctgact ctcattccac aatatttcag gaccggggac
    14521 tgcttcatat cctgaagaag gaaaccactc tgaatatatc cttccccata cattttgaag
    14581 tgtttccggg aaaggcccga cggcttcaaa gactgcccag gtaccggctt ccacattcaa
    14641 tacatcaaat tccgccgttt catcacttgt agttgctgcg ccgatgtaat ggtccagttc
    14701 gcctttttct tccattcttc cttccgagaa gttcgcagag gcattaataa tccctgtcgg
    14761 ttctacattt gacagcgctt tcagtttttc tataatttcg ggggttaaaa gctcagccat
    14821 ttttgcgatt tcgggattta caccgttaaa aataatcggg actctctttt taaatcctac
    14881 gattttaaaa ggtccttttt caacaatacg atatttcatt tcgcatcctc cgttaattga
    14941 taattggaag gtcattcgag gataggcttt aagctggata ttggattttc tcacttcaga
    15001 gggaagaact ccgtgcatgg cacgaaaagc gcgggagaat gaatcgtctg aatcataacc
    15061 gtatttgacg gcaatatcga ttattctcaa gctttcatct ctcaaatcaa gggctgccag
    15121 agtcaatctt ctccttctga tatattccga taaaccaatt cccgacaaaa atgaaaacat
    15181 ccttttgaaa tgatactccg aacagcaagc aattttggag atttcactga aatctatatc
    15241 ttcagtcaaa tgctcttcaa tgtattccat tgctttgttc aaatagctta aagaatccac
    15301 agaacgacct cctttcatca ttaatatatc agaggacgcg tttgttcatc cgacaatctg
    15361 tgcacagctt tgtcggaagg ctattcgctt caaaaattca ctaaaaactt aacccgatgc
    15421 tttggcagct gtatatatcc aatctgcccg tacaaatttt acaaaactac tataacatca
    15481 aaatcgtcca ctcgcgaagg ctaatatctt acctatatac acgcaaagta ctgacaccta
    15541 aaccgaccac gcaaacataa attagaacca tttacgcatc tagtctctac tatcctttat
    15601 ctcattcata tatcctgctg ttattatacc tgccggaagc gctacaatgg caataccaaa
    15661 aacggatgag aacattgtta caattctacc aatggtgctt accggataga tatcaccata
    15721 gcccacagtt gtcaatgata ctgttgccca gtaaactgca tcaaaaaatg tattgaatga
    15781 atcgggctca acattaaaaa ttactaaggc agaaatcaag atatatgcgc cagccagtgt
    15841 acatactgct attaacgcct ctctagactt attaaacacc ttcataataa tgctataatg
    15901 ctcatacttt ttgaatatct tagtacttta aacacccgca gaacccgaga cgcccttatc
    15961 attcgtaaaa tacgaagcag ctttaatcca tcgtttagaa tcgttactga cggtaaaatc
    16021 gcaaccaggt caattattgc cataaaggaa aaggggtatt taataaatga gcttactgac
    16081 attttattgt atttataatc agccgttatt aaccgaagta tgtagtcaca tataaagata
    16141 actaaaacta ctttgtctgc aatttgaaat aacaaagtat ccttcttaaa ggcgagcgga
    16201 ataatactaa tgataattgc agcaatcatt atgcaatcat agatactgct taatctgtct
    16261 ccacctttcg atagttctac catctcaaaa attcgtttcc gcatacgatc accttatatt
    16321 catatatcgc acatatcaga aatttcaaaa ctgttgatcg gaaaagcctg tccttgacat
    16381 caatactacg cactcaacgt ggcacg
//
LOCUS       DAONVQ010000025        16139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2678_length_16139_cov_4.479483, whole genome shotgun sequence.
ACCESSION   DAONVQ010000025 DAONVQ010000000
VERSION     DAONVQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16139
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2678_length_16139_cov_4.479483"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLD98_02845"
     CDS             <1..181
                     /locus_tag="PLD98_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05221.1"
                     /translation="RKTNRVVIQNIVVSLAVKAIILILAAMNLASMWAAVFGDVGVAM
                     LAVLNSVRILRSDKL"
     gene            242..697
                     /locus_tag="PLD98_02850"
     CDS             242..697
                     /locus_tag="PLD98_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQA05222.1"
                     /translation="MKISSKGRYALQIMLDLAQCPPNVYTPIKEIAARQQLSLKYLEQ
                     IMNMLSKAGLLHSIRGPQGGYMLTRKPSEYIIGDILRATEGNLAPVDCIEITDKSCPK
                     LDSCPMALFWNGLHEAVLNYIDSITLADLIEKSRNLGPSFCEGGGQNRE"
     gene            complement(756..1526)
                     /gene="sigG"
                     /locus_tag="PLD98_02855"
     CDS             complement(756..1526)
                     /gene="sigG"
                     /locus_tag="PLD98_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HQA05223.1"
                     /translation="MYFNKVEICGVNTSELKVLKESEKTELLRRVKQGDKAARDALIN
                     GNLRLVLSVIQRFAGRGENLDDLFQIGCIGLIKSIDNFDVTQDVKFSTYAVPMIIGEI
                     RRYLRDNNSVRVSRSLRDTAYKAMQVKEDLQNELNREPDIEEIAERMGIPKSQVVLAL
                     ESIVEPVSLFEPVYSDGGDTIFVMDQVSDRVTDTDWMDEILFKESLEKLSEREKMILN
                     LRFLQGKTQMEVAEEIGISQAQVSRLEKGAVQKIKENG"
     gene            complement(1759..3204)
                     /locus_tag="PLD98_02860"
     CDS             complement(1759..3204)
                     /locus_tag="PLD98_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HQA05224.1"
                     /translation="MSENRIGFKDKIGYAFGDVGSSLIFGLGSTFSMVYYTDVLGLPT
                     AALSIMFLVLKIVDGAIDPFLGMFFDSRRPTKSGKFKKYLVIFAVPVALSAVLMFFPF
                     SYFGINLSGKQAITFASVTYLLYVVTYSSVNVPYGSLASVITDDQKGRTSLSTFRSLG
                     SGLGGLPSALILPMLVYSSVKSVDKVTGESITVQVIKPDMLFWCVIAMSVACVVFLFL
                     SYKMTTERIRLDETQEKTDIAKTLKSLIKSKPFISISLASLVLIMYQTFNVSFLQYLG
                     KDYFGNKAFITLSSLCFYAPFVLLLPFLSKLIERYGKKEVCSAGLFIAAFAHLLLFIL
                     QTKNHILYLALLFVAGTGATIIILQVWALVADVIDYQELIRGKREEATTFSIYFFIRT
                     FGNALSLFFANRFLGLVGYQEAVNGISQIQTEATVKGIYNIAALFPAACCLIMALLLA
                     FLYPLNKKEANSLKEKLKVSRIERGITVSET"
     gene            complement(3226..5730)
                     /locus_tag="PLD98_02865"
     CDS             complement(3226..5730)
                     /locus_tag="PLD98_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05225.1"
                     /translation="MDYKIHVAFGFHVNCYHSYRGDTNDEFGFGGDIRIIRGIINTLN
                     EFNESGVNVKGTWDFENYYSLEKILPEYAPDIIEEVKKRVKKYGDENIIMGYNNGALS
                     AMTDEEFKSSIELAVTNEQKSGLLDIFGEYAPIVRPQEVMFTPSQVSLYNKLGIKALC
                     LYYSCIPFDAFKTLIPPLPEKDAFNFVNYTYKSESIPVLPTISNVDLIDYGSLKFLVK
                     SLHQKQLKGEIDSDLFIFINMDADAVFWEGLKLKGALIKIPNLDGIRGLVNEVKDLDY
                     VVFDTPYNYLKSHPPVAKIEFTQDTADGNFTGYSSWAEKPFNRKIWSRLERARAYSSL
                     YENEKDSPSFKDRILLLSTIHFGLSSPVLNIVREQRALELSEAMVKKELDAAPERENL
                     TLYNIAGSSLVSAQIEVKKGIVKDISALEISSDELLSYGAVANSFYDDGSVKSIFAVF
                     RFESAKKEYPLSIEIKSSNKKSESKPNALKTKSIEVKLSEGGEIRSVFYKGKQIGAED
                     FIKSFINYDYKKYSFSPRKCERLNAAGDIEGISVQGKVSLPYELKSGGFRLELFTVKG
                     IDSLFIKMSVNYPYTEETTAISNENASLGRNCDMKWIETVPLQIKPALYGDISIIKRN
                     YSDDISSFKVSSFWEAVPENKKLDSFNHQITNGFVAVSDGETGLVIANAKQVLNSMAH
                     CPMRLRRKDDKAYVTLNPFGTYFGRQRKHPSASGGAVMDAYMMIATQGKSLAPAYNGA
                     KETFALALFGFDSDRIGYKLLSDIKGFSEGAVVLASKNSPFGPSVEDNAEVANVEPKN
                     TEAKGLGIPVNYKSIKVALKVAKKIVETEIKSALMK"
     gene            complement(5835..6452)
                     /locus_tag="PLD98_02870"
     CDS             complement(5835..6452)
                     /locus_tag="PLD98_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HQA05226.1"
                     /translation="MLISTALINLIMVKGTESRIISLEKAAENGDYDCILVLGAKVMP
                     DGSLSPILKDRVKVGLELYFNGAAPKLLMSGDHGRKEYDEVGAMKNFAISEGGSSADL
                     FMDHAGFSTYESVYRAKEIFGAKRVLIVTQKYHLHRALYIAKRLGLEADGVASDLRDY
                     RSMAYNNTREYLARIKAFGMCIFKPEPTYLGETIPIYGDGNLTND"
     gene            complement(6526..7647)
                     /gene="prfB"
                     /locus_tag="PLD98_02875"
     CDS             complement(6526..7647)
                     /gene="prfB"
                     /locus_tag="PLD98_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078767779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQA05227.1"
                     /translation="MLQFEELRFELLGSKDALINLADAIDLENLKNELKSLEEKMSEE
                     GFWNDIENANKVNKRISALRGKINKYNSLYRLYEDTLTLIELAMEEGDESLYDECKAN
                     VESFKSELEIQTLSTLLTGEYDNNNAIITFHAGAGGTEAQDWALMLYRMYNRWCERHG
                     FKTSTLDYLDGDEAGIKSAVMLVEGQNAYGFLKSEMGIHRLVRISPFDASGRRHTSFA
                     SVEVIPEIDDDINVEIRPEDIKMDVYRASGAGGQKVNKTSSAVRLTHIPTGIVVACQV
                     ERSQHQNREVAMRMLKSKLIEIKERENLEKIEDIKGVQKEIAWGSQIRSYVFMPYTLV
                     KDHRTNYESGNINAVMDGDLDGFINAYLKMQSKGANNNK"
     gene            complement(7762..8754)
                     /locus_tag="PLD98_02880"
     CDS             complement(7762..8754)
                     /locus_tag="PLD98_02880"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HQA05228.1"
                     /translation="MKRIAFFDTKPYDRTWFDALNTKYEIDYFEIKLNERTAVLTKGF
                     DGMIAFVNDEINEKAINTLVENGVKVIAMRCAGYNNVDIKAADKKITIVRVPAYSPHA
                     VAEHTMALLLTLNRKIHKAYLRTRDFNFSLVGLMGFDLYGKTVGVIGTGKIGRAFIDI
                     CKGFGMKVLAYDPYPADIPDVKYVSLDTLLSESDIISLHCPLTEETRHILDEEAFSKI
                     KKGAVVINTSRGELIDSHALLNALNNETLFGAALDVYEEESEFFFEDYSNTIIKDDIL
                     SLLLSRPNTIITSHQGFFTKEAMKSIAETTLKNLDDFFEGRPLKNEVCFRCPNN"
     gene            complement(8788..9999)
                     /gene="hydF"
                     /locus_tag="PLD98_02885"
     CDS             complement(8788..9999)
                     /gene="hydF"
                     /locus_tag="PLD98_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HQA05229.1"
                     /translation="MSLNATPKSERTHIGIFGSINAGKSSIINAITGQDAAIVSDVSG
                     TTTDPVYKSMELLPLGPVVLIDTPGLDDKGQLGKLRVEKSLRVLEQTDIAVVVLDASV
                     GLTEQNRELIEKIEQKNLPYVLVMNKSDTPEAKAYIEKMPEDEKEKYSFVSAKTGEGI
                     WELKEKIASLAPKGNEKRLIADLLKPGDMVILVVPIDSAAPKGRLILPQQQVIRDILE
                     AFAVSVVIRETEVASTINALGQKPRLVVTDSQAFKTVSEQTPPDIQLTSFSILFARYK
                     GNLKQLVEGVKTLDTLKDGDTVLISEGCTHHRQCDDIGTVKLPRWIKNHTKKELNFEF
                     TSGGHFPSDLSKYSLIIHCGACMLNEKEMQYRLSQARESGIPMTNYGTAIAYMHGILK
                     RTLEPFTDITI"
     gene            complement(10017..11453)
                     /gene="hydG"
                     /locus_tag="PLD98_02890"
     CDS             complement(10017..11453)
                     /gene="hydG"
                     /locus_tag="PLD98_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HQA05230.1"
                     /translation="MTVTNAIPYNPKSSKAEEFINHQEVLDTLRYAAENKNNVKLIDE
                     ILKKAAEKKGLSHREAAVLLECDIPEKNKEIFALAKKIKDEIYGNRIVLFAPLYLSNY
                     CVNSCVYCPYHISNKHIPRKKLTQDEIRAEVIALQDMGHKRLALETGEDPVNNPIEYV
                     LESIDTIYSIKHKNGAIRRVNVNIAATTVENYRKLKEAGIGTYILFQETYHKGSYEKL
                     HPKGPKHDYAYHTEAMDRAMQGGIDDVGLGVLFGLELYKYEFAALLMHAEHLEAVFGV
                     GPHTISVPRIRRADDIDPSAFDNGIDDDTFAKIVACIRIAVPYTGMIVSTRESKKVRE
                     RVLELGVSQISGGSRTSVGGYIHPEEDEGTEQFDVSDRRPLDEVVCWLMERGFTPSFC
                     TACYREGRTGDRFMSLVKNGQILNCCHPNALITLKEYLVDYAWPRTKEVGDRFIAEEL
                     NRIPKEKVRHITENYLKEIENGKRDFRF"
     gene            complement(11478..12533)
                     /gene="hydE"
                     /locus_tag="PLD98_02895"
     CDS             complement(11478..12533)
                     /gene="hydE"
                     /locus_tag="PLD98_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HQA05231.1"
                     /translation="MLKLLIDKLEKENRLTKEEFAILIDNATEEDRQYLFSKARKIKE
                     RYFGNKIYIRGLIEFTNYCKNDCYYCGIRRSNKEAERYRLTKDDILSCSETGYSLGFR
                     TFVLQGGEDPYFNDERMTDIVSSIKERYSDCALTLSLGERSYESYKALYEAGADRYLL
                     RHETADSGHYSKLHPEEMLLSTRKEALYALKEIGYQVGTGFMVGSPYQTTEHICTDLE
                     FIADFKPHMVGIGPFIPHHNTPFKDFKAGSVELTLLLIGIIRLMLPCCLIPATTALGT
                     LAATGREMGVLAGANVVMPNLSPSTVRNKYLLYDNKICTGDEAAECRMCLERRMNSIG
                     SVIAVDRGDSPLVTSSI"
     gene            complement(12527..12796)
                     /locus_tag="PLD98_02900"
     CDS             complement(12527..12796)
                     /locus_tag="PLD98_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HQA05232.1"
                     /translation="METRVALIGIIVECPDAAGMLNDVLHDYGKYIIGRMGIPYHKRE
                     INIISIVIDAPLDIINSLTGRLGMIKGISAKAMFAKMPNEKVKNC"
     gene            13117..13204
                     /locus_tag="PLD98_02905"
     tRNA            13117..13204
                     /locus_tag="PLD98_02905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13151..13153,aa:Ser,seq:cga)
     gene            13430..14326
                     /locus_tag="PLD98_02910"
     CDS             13430..14326
                     /locus_tag="PLD98_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HQA05233.1"
                     /translation="MKKVNKQKIITLTLAILFMVSLATNVFLYYKLHMETQKIKISII
                     SSPEKDYFTRMKTLTDYSFINDWSDKGIIFEKLYREKIHSDLKRHEGFIPNRVEAELI
                     AQNPDYPNGCESASAVMLLRYCGFDITLKDFVDNHLKKEEVIDKDGVRYGPDPSKCYA
                     GDPASERRGWGCFAPVITEALTSAIGSENYKAVDISQTSLDFLSLFLPAVVWTTQDYK
                     EATKVYQWKSLDGNFTYTYPVGSHTVLLVGVDKEYVYLNDPLKPEGVQKVKREQFEAS
                     FTSTGRQAVTIINRTPHAEGPY"
     gene            complement(14365..14883)
                     /locus_tag="PLD98_02915"
     CDS             complement(14365..14883)
                     /locus_tag="PLD98_02915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQA05234.1"
                     /translation="MEDGGIIKAELYPEIAPNTVYNFISLANKGCYDGVIFHRVIPNF
                     MIQGGDPNGAGTGGPGYRIKGEFTNNGFENNLKHERGVLSMARQGNPYNPKSAYDTAG
                     SQFFIMVAEAPHLDGDYAAFGKVIEGMDVVDKIVSVKRNLADKPLEDQRMKKVSVETF
                     GVNYPEPETLPK"
     gene            complement(15026..15676)
                     /locus_tag="PLD98_02920"
     CDS             complement(15026..15676)
                     /locus_tag="PLD98_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HQA05235.1"
                     /translation="MLPILKIYTCFLENPLEEDARQRFIKLLPEDRQAKIKAKKNTIA
                     ADESLIGEILARYALSNELLKPLKEIEIRHTEKGMPYIEGDLFFISITHSHGFVACAV
                     SDSPVGIDAEKLRDFSEGVARRMFTENELSFIESSDDKTRSFYMIWTLKESYLKAIGT
                     GISFPMKNLEFTVKDGSISFNLDNNAKFRVIESIDGYILSVCRIAGSNKAHIIEKD"
     gene            complement(15685..>16139)
                     /locus_tag="PLD98_02925"
     CDS             complement(15685..>16139)
                     /locus_tag="PLD98_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05236.1"
                     /translation="VSCDTCRLPNGQTFVIYTTGEAGIFDDKGAVLWTGDILYQDAPA
                     RDAAADGRFVWCVVPKKNAIIKYSPAGKKVVMRIGGDRTTSFSLPGSVAIYDNMLYVS
                     NRGSNKIRTIRLSDFAVEDYRLFEEPVHKYLRVQDKEFVILDSGVYIL"
BASE COUNT         4076 a         3867 c         3588 g         4608 t
ORIGIN      
        1 ccgcaagaca aacagggtgg taatacagaa tatcgttgtc tctcttgctg taaaggcaat
       61 aatcctcatc cttgccgcca tgaaccttgc ctcaatgtgg gcagcggttt tcggcgatgt
      121 gggcgtcgca atgctcgcgg tgctgaattc tgtcagaatc ctcagaagcg acaagctgta
      181 aaggctgtga ttcttgctaa gtcgggcgaa ttgtgctatc ataaaatttg aggtgggtga
      241 aatgaaaata tcgtcaaagg gcaggtacgc ccttcaaata atgctcgact tagctcaatg
      301 ccctccgaat gtttacactc ccataaaaga aatcgcggca agacagcagc tctcgctcaa
      361 ataccttgag cagataatga atatgctcag caaggcgggt ctccttcaca gcattagagg
      421 tcctcagggc ggctatatgc tcacaagaaa gccgtcggaa tacatcatag gcgatattct
      481 gagggcaacc gagggcaatc tcgcccccgt tgactgtata gagattacgg acaaaagctg
      541 ccccaaactg gacagctgcc ccatggcgct gttctggaac ggtctgcacg aagcggtgct
      601 gaactacatt gacagcataa ctcttgcgga cttaattgaa aaaagcagga atttaggtcc
      661 ctccttctgc gaaggcggag ggcagaatag agaataaaaa gaaatgcagg ttttaaacct
      721 gcatttttta tttagaggga gaaaagcaaa tgaatttatc cgttttcttt aattttttgc
      781 actgcgccct tttcgagcct tgaaacctga gcctgagaaa tgcctatttc ctcagcgact
      841 tccatctggg ttttgccctg caaaaagcga agattcaaaa tcattttttc tctttcgctt
      901 agcttttcaa gggactcctt aaacagtatc tcgtccatcc agtcggtgtc tgtcaccctg
      961 tcgctgacct ggtccatcac gaaaatcgtg tcgccgccgt ctgaataaac gggctcaaac
     1021 aaagaaacgg gctccacaat ggattcgaga gccagcacaa cctgtgattt cggtatgccc
     1081 attctttcgg caatctcctc aatgtcgggc tctctgttca gttcgttctg taaatcctct
     1141 ttaacctgca tagccttgta tgctgtgtcc ctaagcgagc ggctgaccct cacgctgttg
     1201 ttatccctga gataacgccg gatttcgcct ataatcatcg gaacggcgta tgtggagaat
     1261 ttaacatctt gagtaacatc aaaattgtct atggatttta taagccctat gcagcctatc
     1321 tgaaacaggt cgtcaaggtt ttcgcctctg cccgcaaacc tttgaataac gcttaaaacc
     1381 agccttaaat tgccgtttat cagcgcgtct cttgcagcct tgtcgccctg cttaacacgc
     1441 cttaatagct cggttttttc gctttctttt agcactttca gctctgatgt gtttaccccg
     1501 cagatttcaa ccttattgaa atacatcctt cttcccccct ccttttatgt acgataaata
     1561 acaggagagg aaatttttgt ttccgctatt aattattgcc tgtccaaaat gagttcattc
     1621 ttttaaaagt taacaacttt acgacagctt tcttctcagc ataaaaatat tttttacttt
     1681 catggcgaaa atataagtgt aaaaaaatga aaaagcggca tcgaaagata ccgcttttta
     1741 tatttaggtt tttatgcttt aagtctcgct taccgtaatg cctctttcaa ttctgctgac
     1801 tttcagcttt tcctttaagg aatttgcctc ttttttatta agaggatata ggaaagccaa
     1861 aagcaaagcc attataaggc agcacgcggc ggggaacagc gcggcgatat tgtatatgcc
     1921 ctttacagta gcctcagtct gaatctgaga tatgccgttc acagcctcct gatagccgac
     1981 taagcctaag aatctgttgg caaagaaaag agataaggcg ttaccgaatg tgcgaatgaa
     2041 gaaatatatg gagaatgtgg tggcctcctc acgcttgcct cttataagct cctgatagtc
     2101 tataacatcg gcaaccagcg cccaaacctg aaggattatt attgttgccc ctgtgcccgc
     2161 gacaaagaga agcgcaaggt aaagaatatg atttttggtc tgcaggatga aaagcagcaa
     2221 atgtgcaaaa gccgcaataa acagacctgc cgagcaaacc tcttttttgc catagcgttc
     2281 aatcagcttt gacaaaaacg gcaacagcag aacaaacggg gcgtagaagc aaagagaact
     2341 gagggtaata aacgccttgt tgccgaaata gtccttgccg agatactgaa ggaaggacac
     2401 attaaaggtc tgatacatta taagcaccaa agaggcaagg gaaatcgaaa taaacggttt
     2461 gctcttaata aggcttttaa gcgtctttgc gatatcggtc ttttcctgag tttcatcaag
     2521 ccttattctc tcagtggtca ttttgtaaga aaggaagagg aaaacaacgc aggcgacgct
     2581 catggcgata acgcaccaga acagcatatc gggctttata acctgaacgg taatgctctc
     2641 gccagtcacc ttatcgacgc tttttacgct gctgtaaacc agcatgggga gtataagcgc
     2701 ggagggaagt ccgccaagcc ccgagcccaa agacctgaag gtcgaaaggc ttgtgcgtcc
     2761 cttctggtcg tccgtaatga cagacgcgag tgagccgtat ggcacattta cgcttgaata
     2821 tgtaaccaca tacaaaagat aagtaacgct cgcaaaggta attgcctgtt tgccgctaag
     2881 atttatcccg aaatagctaa agggaaagaa catcaaaaca gcggacaacg caacggggac
     2941 ggcgaaaatt acaaggtatt ttttgaattt tccgcttttt gtgggccttc ttgagtcaaa
     3001 aaacattccg aggaaggggt caatcgctcc gtcaacaatc tttaatacca ggaacataat
     3061 gctcaaagcc gctgttggca gccccaaaac atcggtatag taaaccattg aaaaggtgct
     3121 gcccagaccg aaaattaagc ttgagcccac atcgccgaaa gcatagccta ttttgtcctt
     3181 aaatccgatt ctgttttcgc tcaccctgct caaattaatt accccttatt tcattaatgc
     3241 ggatttaatc tccgtttcaa cgattttctt tgccacttta agggcaacct ttatggattt
     3301 atagtttacg gggatgccga gtccctttgc ttcggtattt ttaggctcga catttgcgac
     3361 ttcggcgtta tcctctacag agggaccgaa cgggctgttt ttgcttgcca gcaccacagc
     3421 gccctcggaa aagcccttta tgtcagacag cagcttgtag ccgattctgt ctgaatcaaa
     3481 gccgaaaagg gcgagcgcaa aggtttcttt cgctccgttg taagcgggag ccagggattt
     3541 gccctgcgtc gcgatcatca tataagcgtc cataacggca ccgcccgatg cggaggggtg
     3601 ctttctttgc ctgccgaaat atgtgccgaa gggatttaat gtgacatatg ctttatcatc
     3661 tttccttctc agcctcattg ggcagtgagc catggagttc agaacctgct ttgcgttagc
     3721 tattacaaga cccgtttccc cgtcgctgac tgccacaaag ccgtttgtaa tttgatggtt
     3781 aaaggaatcg agctttttgt tttcgggtac agcttcccag aaacttgaca ctttaaagct
     3841 gcttatgtcg tcggaataat tccttttaat aatcgaaata tcaccgtaaa gggcgggctt
     3901 tatctgcaat ggcactgtct caatccactt catgtcgcag tttctgccca aagaggcgtt
     3961 ttcgtttgag attgccgtcg tctcctcggt ataaggataa ttcacgctca tttttataaa
     4021 taagctgtca atgcctttta cagtaaacag ctcaagacgg aagccgccgc ttttaagctc
     4081 ataggggagg gaaactttgc cctgaacgga aatgccctca atatccccgg cggcgtttag
     4141 cctttcgcac tttcggggag agaagctgta ttttttataa tcgtagttaa taaagctctt
     4201 aataaaatcc tcagcgccaa tttgcttgcc cttatagaaa acggagcgaa tttcaccgcc
     4261 ttcgctgagc tttacttcaa tgctctttgt tttaagcgca ttcggcttgc tttcgctttt
     4321 cttatttgag ctttttattt ctatcgacag cggatattcc ttttttgcgc tctcaaagcg
     4381 gaataccgca aaaatgctct ttaccgaccc gtcgtcataa aagctgtttg ccacagcgcc
     4441 atagcttaat agctcatcgg agctgatttc aagagcggag atatccttta caatgccctt
     4501 cttaacctca atttgagccg aaacaagact tgagccggcg atattgtata aggtcagatt
     4561 ctccctttcg ggagcggcat caagctcttt tttgaccata gcctcagaca gctcaagcgc
     4621 cctttgctct ctgacaatgt tcagaacggg ggaggagagt ccgaaatgga tggtggataa
     4681 aagaaggatt ctgtctttaa aggaagggga gtccttttcg ttttcgtaaa gcgaggaata
     4741 cgcccttgcc ctttcaagac ggctccagat tttgcggtta aagggctttt ccgcccagct
     4801 gctgtagccg gtaaaattgc cgtcggctgt gtcctgagta aactctattt tggctacggg
     4861 cgggtgagat ttaaggtaat tgtacggggt atcaaatacc acataatcga ggtctttaac
     4921 ctcattcaca agacccctta tgccgtcaag gttggggatt ttgatgagag cgccctttaa
     4981 cttcagcccc tcccagaaaa cagcgtcagc gtccatatta ataaatatga aaaggtcgct
     5041 gtcaatctcg cccttcagct gcttttggtg aagggatttt acaaggaatt ttaaagagcc
     5101 gtaatcgata aggtccacat tactgattgt tggcagaacg ggaatgctct cgctcttata
     5161 tgtatagttt acaaagttaa aggcgtcctt ttcgggcaga ggaggaatca gcgtcttaaa
     5221 ggcgtcaaag ggtatgcagc tgtaataaag gcacaaagcc ttaatgccaa gtttgttgta
     5281 taaagaaacc tgcgacggag tgaacataac ctcctggggt ctgacaatcg gagcatattc
     5341 gccgaaaata tcaagaaggc ccgatttttg ctcattggtg acagcaagct caattgagct
     5401 tttgaattct tcgtccgtca tggcggacaa agcgccgttg ttatagccca ttattatgtt
     5461 ctcgtcgccg tattttttga cccttttttt aacctcctca ataatgtcgg gagcgtattc
     5521 gggcagtatc ttttcaaggg aatagtagtt ttcaaaatcc catgttccct tgacattaac
     5581 accgctttcg ttaaactcgt tcagcgtgtt tataatgcct cgtataatcc tgatatcccc
     5641 gccaaagccg aactcatcgt tcgtgtcgcc tctgtacgag tgatagcagt ttacatgaaa
     5701 accgaaagcc acatgaatct tgtaatccat atttcctccg cctaatgtaa atttaaataa
     5761 attatacaaa ttttatccat tttatgcaat aagcaataaa aaaacggcca aagcagttag
     5821 ctttagccgc agttttagtc gtttgtaaga tttccgtcgc cgtaaatggg aatggtctcg
     5881 ccaagatatg tgggctcggg cttgaaaatg cacatgccga aagccttgat tctcgcaagg
     5941 tactcccttg tgttattgta tgccatgctc cgataatctc taaggtcgga ggcgactccg
     6001 tctgcctcca gtcctagcct tttggcgata tacaaagccc tgtgcaggtg gtatttctgc
     6061 gtcactatca aaacccgctt tgcgccgaaa atctccttag ccctgtatac cgactcataa
     6121 gttgaaaagc ccgcgtggtc cataaaaagg tcagccgagc tgccgccctc gcttatggca
     6181 aagtttttca tggctcccac ttcatcgtat tctttcctgc cgtggtcgcc gctcattaag
     6241 agctttggcg cagctccgtt aaagtaaagc tccaaaccta ccttaaccct gtctttgagt
     6301 atggggctta gacttccgtc gggcatcact ttcgcgccca gaaccaaaat gcagtcgtaa
     6361 tccccgtttt cagcggcctt ttccaaagag attattctgc tctcagtgcc cttgaccata
     6421 attaaattaa tcaatgcggt ggagatgagc acgaaaaccg ccgccccgca aacgaaaata
     6481 gcggagcgtc ttatgaattt tccctttaaa aagccgccgt tagttttatt tattgttatt
     6541 cgcccctttt gactgcatct ttaaatatgc gttgataaag ccgtcaaggt ctccgtccat
     6601 tacggcgttg atatttccgc tctcgtagtt ggttctgtgg tccttaacaa gggtgtaggg
     6661 catgaagaca tatgacctta tctggctgcc ccaggcgatt tccttctgaa cgcccttgat
     6721 atcctcgatt ttctcaaggt tttctctttc cttaatctca attagctttg atttcagcat
     6781 cctcatggcg acctcgcggt tctgatgctg gcttctctca acctggcagg caacgacaat
     6841 gcccgtgggg atatgggtaa gtcttaccgc agaggaggtc ttgttaacct tctgaccgcc
     6901 cgcgccgctt gccctgtaaa catccatctt gatgtcctcg ggtcttatct ccacattgat
     6961 gtcgtcgtca atttcgggaa tgacttcaac ggaggcaaag gatgtatgcc ttctgcccga
     7021 cgcgtcaaag ggagaaatgc ggacaagtct gtgaataccc atctcgctct ttaaaaatcc
     7081 gtaggcgttc tgaccctcca ccagcataac ggcggacttt atacccgcct catctccgtc
     7141 aagatagtcc agtgtggatg tcttaaagcc gtgacgctcg caccagcggt tatacattct
     7201 gtaaagcatg agcgcccagt cctgagcctc tgtgccgccc gcgcccgcgt ggaaggttat
     7261 aatggcgttg ttattgtcat actcgccggt taatagggtg gaaagggtct gaatttcaag
     7321 ctcggactta aagctctcca cattagcctt gcactcgtcg taaaggctct cgtcaccttc
     7381 ctccattgca agctctataa gcgttagggt atcctcataa aggcggtata aagaattgta
     7441 tttgtttatc tttcctctca gtgcgcttat tctcttattt accttattgg cattttctat
     7501 atcgttccaa aatccctctt cgctcatttt ctcctcaagg gatttaagtt cgtttttaag
     7561 attttcaagg tctatggcgt ccgcaagatt tataagggca tccttgctgc ccaaaagctc
     7621 aaatctcagc tcttcaaact gaagcataaa ttacccctta aatttaagtt ttgcacttca
     7681 atataataca ctaaagctta aaaggctgtc aacaaaacta atgccgagag caaactgcta
     7741 tcggcattta tcggtttgga tttaattgtt cgggcagcgg aagcaaacct cattttttag
     7801 gggtctgccc tcaaagaaat catcaaggtt tttaagcgtg gtctccgcga tgctcttcat
     7861 cgcttccttg gtaaaaaagc cctgatggga ggtgatgatg gtgttcggtc ttgagagcag
     7921 gagagagagg atatcgtcct tgattatcgt attggagtaa tcctcaaaga agaactccga
     7981 ctcctcctcg taaacatcaa gcgccgcgcc gaaaagggtt tcgttgttga gagcgttcag
     8041 cagcgcatgg ctgtctatca gctcgcccct tgaagtgtta atgacgaccg cgcccttttt
     8101 gattttggag aaagcctcct cgtcaagaat atgccttgtc tcctcggtga gcgggcagtg
     8161 aagtgatatg atgtcgcttt cagagagaag cgtgtcaaga gacacatact taacatcggg
     8221 aatgtcggcg gggtaggggt cgtacgccag aaccttcatt ccaaagccct tgcatatgtc
     8281 gatgaacgcc ctgccgattt tacccgtgcc tataacgccc accgtcttgc cgtaaaggtc
     8341 aaagcccata agtcccacaa ggctgaagtt gaaatccctt gttctcaaat atgccttgtg
     8401 aatcttgcgg ttaagagtta aaagcagagc cattgtgtgc tcagcgaccg catggggaga
     8461 gtaggcgggc actctgacaa tggtaatttt tttgtccgcc gccttgatat ccacattgtt
     8521 gtagcccgcg cagcgcatgg cgatgacctt tacgccgttt tccacaaggg tattaatcgc
     8581 cttttcgtta atctcgtcat tcacaaaggc aatcatgccg tcaaagccct ttgtcaaaac
     8641 agcggttctt tcgttcagct ttatttcaaa gtaatcgatt tcatattttg tgttaagagc
     8701 gtcaaaccat gttctgtcgt agggtttagt atcgaaaaac gctattcttt tcatatccat
     8761 caccccctat tatgtcccgt tttgttatta aatagtaatg tcagtaaagg gttcaagggt
     8821 tctttttaag attccgtgca tatacgcgat ggcggtgccg tagttggtca tgggaattcc
     8881 gctttctctt gcctgagaaa gtctgtactg catttccttt tcgttgagca tgcaagctcc
     8941 gcagtggata attaggctgt atttgctcaa atcgcttggg aaatgcccgc cgcttgtaaa
     9001 ttcaaagttc agctcctttt tggtgtgatt cttaatccat cttgggagct taaccgttcc
     9061 tatgtcatcg cattgcctgt ggtgggtgca gccctcgctt ataagtacgg tatcgccgtc
     9121 tttgagagtg tcaagggttt tgacgccctc caccagctgc tttaaattgc ccttgtagcg
     9181 ggcaaagagg attgagaagg atgtaagctg aatatcaggc ggagtctgct ccgatacggt
     9241 tttgaacgcc tggctgtcag tgacaacgag ccttggcttt tgacccaaag cattgattgt
     9301 gcttgcgact tctgtctctc gaatcacaac cgatacggcg aatgcctcaa gaatgtccct
     9361 tataacctgc tgctgaggca gaatcagcct gcccttggga gcggcggagt ctatgggcac
     9421 tacaagtatt accatatccc ccggctttaa caaatcggca atcagccttt tttcatttcc
     9481 cttgggcgca agggaggcga ttttctcttt aagctcccaa atgccctcgc cggtctttgc
     9541 gcttacaaag ctgtattttt ccttttcgtc ctcgggcatt ttttctatgt atgcctttgc
     9601 ctcgggagtg tcggatttat tcatgacaag aacatagggg aggttctttt gctctatctt
     9661 ttcgataagc tccctgttct gctccgtaag acccacagac gcgtcgagaa ccacaacagc
     9721 tatgtcggtc tgctcaagca ctctaagact cttttccacc ctgagttttc ccagctgacc
     9781 cttgtcgtca agtccggggg tgtctataag cacaacggga cctaagggta aaagctccat
     9841 tgatttgtaa acggggtcgg tggtagtgcc gctgacatcg gacacaatcg ccgcgtcctg
     9901 acccgtaatc gcgttgataa tgctcgactt gccggcatta atgctgccga aaattccgat
     9961 atgagttctt tctgactttg gtgtagcgtt aaggctcata tattaactcc tcgaatttag
    10021 aatctaaaat cccttttgcc gttttctatt tctttgagat aattttcggt tatatgcctt
    10081 accttttctt tgggtattct gttaagctcc tcggctatga atctgtctcc gacctctttt
    10141 gttctcggcc acgcatagtc taccaaatac tctttgagag taatcagagc gttgggatgg
    10201 cagcagttga gaatctgacc gttctttaca aggctcataa atctgtcgcc cgttctgccc
    10261 tctctgtagc aggctgtgca gaaactgggg gtaaagcccc tttccataag ccagcataca
    10321 acctcgtcaa ggggtctgcg gtcggaaaca tcaaactgct cggtgccctc gtcctcctcg
    10381 gggtggatat atccgcccac gctggttctt gagccgccgc tgatttgcga tacgcccagc
    10441 tcaagcactc tttcacggac cttcttgctc tcccttgttg aaacaatcat gcccgtatag
    10501 ggaacggcaa ttctgatgca agccacaatc ttcgcaaagg tgtcgtcgtc aatgccgttg
    10561 tcaaaggcag acgggtcaat gtcgtcggcg cgcctgattc tcggaacgct gattgtatga
    10621 ggaccaacgc cgaaaaccgc ctcaaggtgc tcggcatgca ttaagagagc ggcgaattcg
    10681 tacttgtaaa gctcgagtcc gaaaagaacg cccagtccca catcgtctat gccgccctgc
    10741 attgccctgt ccatagcctc agtgtggtag gcatagtcgt gcttcggtcc cttcgggtgg
    10801 agtttctcgt agctgccctt gtgataggtc tcctgaaaga ggatgtatgt gccgattccc
    10861 gcctctttaa gcttcctgta attctcaacc gtagtcgcgg cgatattgac attaactcgc
    10921 ctgatcgcgc cgttcttgtg cttaatgctg taaatggtgt caatgctttc gagcacatac
    10981 tcaatcgggt tgtttacagg gtcctcgccc gtctcaagag ccagacgctt gtgacccata
    11041 tcctgcaaag ctataacctc agcccttatc tcatcttgcg ttagcttctt tctcggaata
    11101 tgcttgtttg aaatatgata agggcagtaa acgcatgagt ttacgcagta gttcgaaaga
    11161 taaaggggag cgaaaagaac aattctgttg ccgtagattt cgtccttaat tttctttgcg
    11221 agggcgaaaa tctccttgtt cttttcggga atatcgcact ccagcaatac cgcagcctca
    11281 cggtgcgaaa gacccttctt ttccgccgcc tttttaagaa tctcgtcaat gagcttaaca
    11341 ttattcttgt tttcagcggc ataccggaga gtatccaaaa cctcctgatg atttataaac
    11401 tcctcagcct ttgaggattt gggattgtag ggaattgcat tggtaacggt cattataagg
    11461 acttcctttc gaaaaaatta tattgagctt gtaaccagcg gcgaatcgcc tctgtcaact
    11521 gcgattacgc tgccgatgct gttcatgcgt ctttctaggc acattctgca ctccgccgcc
    11581 tcgtcgcccg tgcagatttt gttgtcgtaa agcaggtatt tgtttctgac tgtggacggc
    11641 gagaggttcg gcattaccac attcgccccc gcaagaacgc ccatctcgcg acccgtcgct
    11701 gccagggttc cgagagctgt tgttgcggga ataagacagc aggggagcat taagcggata
    11761 attcctataa gcagtaaagt aagctccacg ctgccagcct tgaaatcctt aaagggcgtg
    11821 ttgtggtggg ggataaaagg cccaatgccg accatatggg gtttaaaatc agctataaac
    11881 tcaaggtcgg tgcatatgtg ctcggtagtc tgataaggcg agcccaccat aaagccggtg
    11941 cctacctgat agcctatctc tttaagggcg tacagagcct cttttcgtgt gctgagaagc
    12001 atctcctcgg ggtgcagctt tgaataatgc ccgctgtcgg cagtttcatg ccttagcaga
    12061 tacctgtcag cgcccgcctc atataaagcc ttgtagcttt cgtaacttct ttcgccaagt
    12121 gaaagagtca gcgcgcagtc gctgtatctc tcctttatag aggacacaat atccgtcatg
    12181 cgttcatcgt taaagtacgg gtcctcgccg ccctgaagca caaaggttct aaaacccagg
    12241 gaatatcccg tctcactgca ggagagaata tcatccttag taagtctgta gcgctctgcc
    12301 tctttgttgc tgcgccttat gccgcagtag tagcagtcgt ttttgcagta gtttgtaaac
    12361 tcaataagac cccttatgta tattttgttg ccaaaatatc tttccttgat tttgcgagcc
    12421 ttagagaaaa gatactgtct gtcttcctca gtggcgttgt caatcagaat tgcgaattcc
    12481 tcttttgtaa ggcggttttc cttttccagt ttatctataa gtaactttaa caattcttca
    12541 ccttctcatt cggcattttg gcgaacatag cctttgctga aatgccctta atcatgccca
    12601 gcctgcccgt aagagagtta atgatgtcca gcggggcatc aattactatg ctgataatat
    12661 tgatttccct tttgtgatag ggaatgccca ttctgccgat gatgtatttg ccgtagtcgt
    12721 gcagaacatc attgagcatt cccgctgcgt cgggacactc gacaattatg ccgattaaag
    12781 caactctggt ttccatttgc cgcatccttt cgtgaaatct cttgctttgg gctaaagaaa
    12841 caaaaaacac cccgcaaggg ggtgtcaata taccgcgaca tccgccttgc ctttagcctc
    12901 aagcgtaact ttcagcatca gttcaagtcg ttggtttaac cgaaattcgg catcggcaaa
    12961 tagcctttgc cttatttctt gtattctttt gttattatat taacaccgga cacaaagtat
    13021 tgcaagatta atggaacaaa atcacaaaaa catttttttg attgctcttg cacataatga
    13081 ggttttatgc taaaatctct aagctgaatt tcacacggag agttgtccga gtggtcgaag
    13141 gtgcagcact cgaaatgctg tgtggggaaa ctcacccagg gttcgaatcc ctgactctcc
    13201 gccaaaatcc cgcaagtctt aaatttaggc ttgcgggatt ttctttgttt ttccgtaaaa
    13261 tttcctttaa ttacatcttt cgaaattatt cacagaatat taaaaactcc cgtttgcaat
    13321 gtgaattaat tgcagcagtg caaaaaactg ctgcgaaaat cggggcataa agcagctgct
    13381 atcttttata ctaagtccaa ctaatgattt gttttaggga gaaaatagga tgaaaaaggt
    13441 aaacaaacaa aaaataatca ccctaaccct tgctatactg tttatggttt ctttggcgac
    13501 taatgtattt ctgtactata agctgcatat ggaaactcaa aaaatcaaaa ttagcataat
    13561 atcaagcccc gaaaaagatt attttaccag aatgaaaacc ttgacggatt acagcttcat
    13621 taacgattgg agcgacaagg gcattatctt tgaaaagctt tacagggaaa agatacattc
    13681 tgaccttaaa agacatgagg gattcatccc gaatcgcgtt gaggcagagc tgatagccca
    13741 gaatcccgat tatcccaacg gctgcgaatc cgcctcagcg gtgatgctgc tcagatactg
    13801 cggctttgac attactttaa aggattttgt ggataaccat ttgaaaaaag aggaagtaat
    13861 cgataaagac ggcgtgagat acggtcccga cccgtcaaaa tgctatgcgg gcgaccccgc
    13921 aagcgaaagg agaggctggg gatgctttgc cccagttata accgaggctc tcacctcagc
    13981 aataggcagc gaaaactaca aggctgttga tataagccag actagtcttg attttctttc
    14041 tttattcctg cccgcagtag tctggacaac acaggactac aaagaggcaa cgaaggtata
    14101 tcagtggaaa tcattggacg gaaacttcac ttatacctat cccgtcggct cacatacggt
    14161 tttgcttgtc ggcgtggata aagaatatgt gtatctgaat gaccccttaa agcctgaggg
    14221 agtgcagaag gtaaaaagag agcagtttga agcgagcttt acctcaactg gacgccaggc
    14281 tgtaacaatt ataaacagaa cgccccatgc cgagggcccc tattaaaaaa ataagacggt
    14341 caaacgaccg tctttttttg ttttttattt cgggagtgtt tcgggctctg ggtagtttac
    14401 gccaaaggtt tcgacgctta cctttttcat tctttggtcc tctaaaggct tgtcggctaa
    14461 attccttttg acggatacaa tcttatccac aacatccata ccttcaatta ctttgccgaa
    14521 tgcggcatag tcgccgtcaa ggtggggagc ctcggcaacc ataataaaga actggctgcc
    14581 ggctgtatcg tatgcggatt tgggattgta ggggttgccc tgccttgcca tggacagtac
    14641 gcccctttcg tgctttaagt tgttctcaaa gccgttgttg gtaaactcgc ccttgattct
    14701 gtagccggga ccgcctgttc cagcgccgtt ggggtcgccg ccctgaatca taaagtttgg
    14761 aataaccctg tggaagataa ctccgtcata acagcccttg ttcgcaaggg agatgaaatt
    14821 gtaaacagta ttcggggcaa tttcggggta aagctccgcc ttaattatgc cgccgtcctc
    14881 catttcaatt gttacaatcg gattcttttc aataaccaac ttgctttctc ctttatcgtc
    14941 gctttttcct gagcatgccc ataaagtgag cagcgtggta agtaaactta aggcaaagag
    15001 aatcttaaaa gattttttca tcctctcaat ccttttcaat aatatgagct ttattagacc
    15061 ctgctatgcg gcagacggat aaaatatatc cgtctatgct ttcaatgacc ctaaatttcg
    15121 cattattgtc aaggttaaag cttatagagc cgtctttcac agtgaactcc agattcttca
    15181 tagggaagga aatcccggtg cctattgcct ttaaatagct ttctttcaaa gtccaaatca
    15241 tataaaagct gcgagttttg tcatccgatg attcaatgaa ggaaagctca ttttcagtaa
    15301 acatccgcct tgccacgccc tcggagaaat ctctgagttt ttcagcgtca atgccgacag
    15361 ggctatcgga aacggcgcag gcaacaaacc cgtggctgtg tgtaatgcta atgaaaaata
    15421 aatctccttc aatatagggc ataccctttt cggtatgcct tatttctatt tcttttaaag
    15481 gctttagaag ctcatttgaa agagcgtacc ttgccagtat ttcgccaata aggctctcgt
    15541 ccgccgctat agtattcttt ttggctttga ttttggcctg cctgtcctcc ggcaaaagct
    15601 ttataaacct ttgccttgcg tcttcctcaa ggggattttc gagaaaacag gtataaatct
    15661 taaggatagg cagcaccgcc ttgactatag tatgtaaact cccgaatcga gaatcacgaa
    15721 ctccttgtcc tgaactctta aatatttatg cacaggctcc tcaaacagcc tgtaatcctc
    15781 gaccgcaaag tcggaaagcc tgatggttct aatcttattc gagcccctgt tgctgacata
    15841 gagcatattg tcataaatcg caacagagcc gggcagggag aaggatgtag tcctgtcgcc
    15901 gccgattctc ataacgacct ttttgccggc gggagagtac tttattatcg cgtttttctt
    15961 tggcacaacg caccagacga acctgccgtc ggcggcagcg tcccttgcgg gcgcatcctg
    16021 atagagaata tcgcccgtcc agagcaccgc gcccttgtcg tcgaaaattc cagcctcgcc
    16081 cgtggtgtag attacaaagg tctgaccgtt gggcaggcgg caggtgtcgc aggaaacat
//
LOCUS       DAONVQ010000026        16129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2681_length_16129_cov_5.018913, whole genome shotgun sequence.
ACCESSION   DAONVQ010000026 DAONVQ010000000
VERSION     DAONVQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16129
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2681_length_16129_cov_5.018913"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /gene="hcp"
                     /locus_tag="PLD98_02930"
                     /gene_synonym="priS"
     CDS             <1..1337
                     /gene="hcp"
                     /locus_tag="PLD98_02930"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HQA05237.1"
                     /translation="SDKSDFGDAAVFTVDSGESMLKKAESVGVLSTEDEDIRSLKEMI
                     TYGLKGMAAYTEHAFNLGKEDLEIIEFMYNTLAELLRDDLTADELVALTLKTGEYGVK
                     AMALLDEANTSRYGNPEITEVNIGVRKNPAILISGHDLTDLEQLLEQTQGTGVDVYTH
                     SEMLPAHYYPFFKKYENFAGNYGNAWWKQVDEFGPFNGPILFTTNCIVPPRKEEIRKR
                     IFTTGSAGFPGCKHIEADENGKKDFSEIIELAKTLPAPEEIETGKIIGGFAHNQVFAL
                     ADKVVDAVKSGAIRKFFVMAGCDGRMKSRAYYTEFAEKLPKDTVILTAGCAKYRYNKL
                     PLGDIGGIPRVLDAGQCNDSYSLALIALKLKEIFGLSDINELPLAFNIAWYEQKAVIV
                     LLALLYLGVKNIHLGPTLPGFLSPNVAKVLVEKFGIAGIDTVDSDIEMFMAQ"
     gene            1486..2097
                     /locus_tag="PLD98_02935"
     CDS             1486..2097
                     /locus_tag="PLD98_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HQA05238.1"
                     /translation="MTTGLRFMVGPDEKILYQGKPDLKCFVFETIFNPSLPIVILWGA
                     LDLFVLGNLIFAKGSGKSFFVGFILIHMTPVWIYLSSIIFMARKYRNIEYIVTDKAIY
                     VSGGVFSRTYRSKPFAELSHIDLHRSVFDQLFNVGDVIANTSHIRPNQKSATITLNSI
                     LNFTEVYNLIKKLQQDIYTDIMFPNEYRPDVNRGYKTEYKGFE"
     gene            complement(2134..2694)
                     /locus_tag="PLD98_02940"
     CDS             complement(2134..2694)
                     /locus_tag="PLD98_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQA05239.1"
                     /translation="MNQTAFDREITCKQIDLSNKLRELWNEHVLWTRSFIESTAFDLP
                     DLQAVTDRLLQNPCDFAKLFCRFYGEAKAQQLKKLLKDHLLIAAELVNALKERNVIKA
                     DKIRCKWYENAEDIAKFLASINPYWGKLQWRCLLFDHLRMTEDEAVFILRGQYRKGVT
                     IYDEIQNQAMKMADVMTQGLIRQFNI"
     gene            complement(2821..4347)
                     /locus_tag="PLD98_02945"
     CDS             complement(2821..4347)
                     /locus_tag="PLD98_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05240.1"
                     /translation="MSYNNAIRPYPECCNHSAEPDRRDDCKHDKKKPKCKRCGNLVQD
                     FSFESQYGPWVASNVTFTNTDVYEGAIQARLGPGVASLYQIVSLEGVCRSPMLFSFNA
                     VGSPLLGVQQELTLGILIAEVAWLDSNYNQIGLGLRMVIPGGRINSTAKITFFAQTDR
                     PPEDTAFAKISFSKGSPSFMSEADFIFIDGVLLAPMAYTDLIRNGDFEANLFEWVANP
                     GGDTAFLSSYKESLEGAGHALTQVNGNLSQDISVGHLPPQTSFLLSFAAQGTGNVGLT
                     VRIEWLDYNDNPIGSGLNVSIPNETLNNQQNYLSYLFVSYPMVPGTATARLTFYATVP
                     TTADFLRLDNVIFAPVLTQNLVNNPSFEDGLNYWQQSLVNLIERNDVYEGTADAGIGQ
                     IGGALWQDVDLYDPEGKCYLFSTGLGFRKTIETADFGTMIMKVIWLDRFDRELGVGLS
                     LVSNRVLPTDTNTFIEWIPFAGVTEPAPAGTVRARILFSKTDSEGFIEVDNVVFAQLV
                     "
     gene            4536..5711
                     /locus_tag="PLD98_02950"
     CDS             4536..5711
                     /locus_tag="PLD98_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HQA05241.1"
                     /translation="MNIVDLKQYIKPGKLYKEKESTFYAQAQIPTECRSNHASCLTQL
                     DNGDILCVWFGGSAEGSGDTKIHLSRLNKGASQWTEPKRISDDYNCSEQNPFIYDFGG
                     GHLWLVHTAQKTRGMSKEEWNALVKSGKAKGSYTMQETAEIRILESFDYGYTFKKREP
                     LNQKSGAFCRQPIIRLSNGDLLMGMWYSVVREGDFERGETYGGDYSVCLISEDNGATW
                     KEYPVPDSVGRVHMQPIELENGRLIALFRSRFADRIYKSYSEDYGRTWTAPVPTVLPS
                     NNSSIQAKKLQSGNIALVYNHTPYIANDASKVRWPANSRHHLAVALSEDGGETFPYIR
                     VIEHGEEFFGENWRKNRECSYPSIIEADDGKLHVSYTYNGRRCIKHVIIDENWIKGE"
     gene            5713..6615
                     /gene="dapA"
                     /locus_tag="PLD98_02955"
     CDS             5713..6615
                     /gene="dapA"
                     /locus_tag="PLD98_02955"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HQA05242.1"
                     /translation="MATLKGIVPALITPVTHDEKINEKALRKLANRLIDAGVHGLFAL
                     GTTGEFFSLSQKEKLTAISTVIDEAAGKVPVVIGTGAVSTRESIELSKKAEEMGADYL
                     SVITPYFNCLSQRELSEHYLRIASAVSIPIILYNIPARTGLSLSDDTVSELARVPNIV
                     GIKDSSGKFDNILSYIEVSKGLNFSVYAGTDSLILKTLMAGGSGAVAATACVFPELVV
                     SIYEFWKRGDMEKAEKAQEMLFAIRDFSAKFSIPAAYKAALEIQGIDAGPPISPALQL
                     PYEEKEKLKNLLKKYEKEISELLS"
     gene            6727..7890
                     /locus_tag="PLD98_02960"
     CDS             6727..7890
                     /locus_tag="PLD98_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA05243.1"
                     /translation="MVSLLLAIIYLAFISLGLPDSLLGSAWPVMYEQFGVPLSYAGII
                     SMIISGGTIISSLMSDRLTRKFGAGLVTAVSTLLTALALFGFSLSRSYIPLCVLAVPY
                     GLGAGAIDAALNNYVALHYASRHMSWLHCFWGVGAAISPYIMGYSLSKDLNWSLGYRI
                     VSIIQICFTAVLFISLPLWRKNRENGHTTENSRPLTLKEAVSIKGVKLVLAAFFSYCA
                     LEWTACLWASSYLVEYRQIDANTAARFASLLFIGITVGRFLCGFIADKVGDKNLIRIG
                     TAVTMFGIVLVALPLKVDSIALAGLIIIGFGCAPIYPSIIHSTPVNFGKENSQAIVGI
                     QMASAYVGSTFMPPVFGMLADNISMGLYPFYLMFFGVLMVIMSERLNKTVAKA"
     gene            complement(7919..8914)
                     /locus_tag="PLD98_02965"
     CDS             complement(7919..8914)
                     /locus_tag="PLD98_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HQA05244.1"
                     /translation="MFPDKVYFEPKALEYKLGKELREKYKDVPWIPIESHNNIEELRK
                     NENKEFARMKRHLIIGVRKSLKHVPNNKTSDFLVPYTSSGCSAMCLYCYLVCNYNKCS
                     YLRLFVNREEMMFKLLKEAEKADRDLVFEIGSNSDLLLENTITGNLVWTIENFAKAQK
                     GFITFPTKFDMIEPILPLEHKGRTIVRMSVNPEEIIRKIEFGTSQLSGRIKAVNSLCE
                     ADYPIGLLIAPVVFVSDWKRLYSELIERLADELTEKAKRKIKLEIIFMTYSFIHRSIN
                     NEAFPKAIELFDKVKMTGRGKGKYWYKESLRHEGEEFLRNELSKHLNGVPIAYIV"
     gene            9053..9991
                     /locus_tag="PLD98_02970"
     CDS             9053..9991
                     /locus_tag="PLD98_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQA05245.1"
                     /translation="MAKKLCALLLVVSMLFTIAATGLAADAKPFEDSEFFEIGDYSIH
                     YRFKPATGEFKGRFVLIHGLMSSTTAWEPFAEIMTAEGYDCLLVDLPNFGYSTRENVK
                     VQAIPREDILAALMEELAPGEKWFVGGHSMGGGVAMVLASKHPDLIEALFLYAPGLNT
                     KAMFPNNSEMSLQIIGSIFNAFFKMLVSFARIPFVSQMIKKNVGYAPDFDLNRRYLDA
                     LSVENTGMGLIFMMKRATPLDVEALHELNMPIFLCLAEDDKIVPVDGEMAIKIADNLP
                     EHTVKLIMPGLNHSFIESDADRVTAATLDFIAGLAA"
     gene            complement(10057..12252)
                     /locus_tag="PLD98_02975"
     CDS             complement(10057..12252)
                     /locus_tag="PLD98_02975"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HQA05246.1"
                     /translation="MNKRDKAFLKRISEELKKDTEKFELPETLKPESIAQLLKENSKA
                     PEINEKEVKKGIYVAWAPSKRVIAAAAAFVIIAAAAVFYIGLDRPPSEIQETAAETEA
                     GLSGDIGSSSFDFKESDKIGNVPADTNLSSVSVTQENNEKEVKGVKKANSYSEIEESI
                     WKARKKAQNSSKFSLKNFFGGLFSFKKVGDIVREDNWLDTPSGSESVGYGRTNEQVEG
                     VAEADIIKNDGRYLYIATQYGVRIVDTANNTLKHTSFIRLGQITKEAFTSFEKSTTVA
                     DEYFTRPQNHRILEIFVQGNRLAAIFDYYRPMRVNDTIRQGKSVTGVTVFDISDRSSP
                     KELLHYTQDGYYVSSRIYNGSLSLVTSYDVYYIEGEEEVTKKISIPVIEIQGKADRLA
                     PESIYIPEKSDYQSYLVVGLIKSLEKNSKIDTKAVLGGADSIYQRNENLYTARSIYDG
                     GVRTFEFRSDMVFTDADETEILYFTLKDGNIRLNSRNTVSGGLLNQFAMDEKDGYFRI
                     ATGDSRGNNLFVLDKNLNEVGKVTGFAKGERIKSVRFMGDTAYVVTFVLTDPLFVFDL
                     HDPRNPKIVGELKIPGFSEYLHPVGDNLLIGIGLDGTDTGINGDAKISLFDVSDPTKP
                     IEIDHITYPSARISSNHKAFLTIPEKNLYVVTVMQYRQTSAGQLSVLRSFSVENRRIV
                     PKLTYSPAPGIDELRGTYIGSTLYAIDPMWGIWAMDIDSAEVLYIDFFK"
     gene            complement(12265..12789)
                     /locus_tag="PLD98_02980"
     CDS             complement(12265..12789)
                     /locus_tag="PLD98_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQA05247.1"
                     /translation="MTDVKSNVQKAMSGDADAFAQLYSLYAADLYRFALCYLKNEKDA
                     QDAVQDAVLLAFSKIGSLRNEGAFKSWLFKILSNCCKTRLMKNSAAPVFVDISSAEHL
                     GSDEYLPASLKSELLEVLDTLKDEEREIVLLSALAGYKSHEIAKMLGCPSSTVRSRLA
                     RALKKMRDQLNYGL"
     gene            complement(12940..13992)
                     /locus_tag="PLD98_02985"
     CDS             complement(12940..13992)
                     /locus_tag="PLD98_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA05248.1"
                     /translation="MPDKINVAVVGYGGMGAHHVRELLKMPNYNVKGIYDIKEERCEL
                     ARQNGIHAYNSLDELLADDVELITIATYNEFHKPIAIRAMEAGVNVISEKPVTISLSD
                     LEEMIEASERTGKLFTVHQNRRWDDDFLTAKEIIESNTLGRIFRIESRVQGSRGIPGD
                     WRAEKAHGGGMVLDWGVHLFDQILMLNLDNPVVSVHANLSNVTNEEVDDGCYVTFTFE
                     NGLVCYVEVATSNFISLPRWYILGENGTAVIEDWDLNGKIVKIKDWEKIDAVPIKAGA
                     GITKTMAPRTSETIKEFPLPKVTADWSDYYINIYDVLRNGAQQIVTHDQLRRSMKAIE
                     AVFESAEKNTVVKCRI"
     gene            complement(14076..15041)
                     /locus_tag="PLD98_02990"
     CDS             complement(14076..15041)
                     /locus_tag="PLD98_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQA05249.1"
                     /translation="MKLGVFTTLLSNLSLEEALKYFTSLGIEMVEIGTGGYPGNAHAN
                     PDVLLNDEAEFKKFMDTIKKYNVEISAFSCHGNPVHPDKETAKYYDEVIRKTILLCEK
                     VGIHQINTFSGCPGDHPGAKYPNWVTCPWPNDYLEILDYQWNEVLIPYWKDLVAFAKE
                     HGVNKIALELHPGFAVYNTDSLLKLREAVGPEIGANLDPSHLIWQGMEPVAVIRKLGD
                     AIFHFHAKDTKIDKYNTAVTGVLDTKGYGDVLNRSWTFRSVGYGNDLVYWKDIISNLR
                     LVGYDYAISIEHEDALMSQNEGLSKAVATLKEAIMTETPGEMWWV"
     gene            complement(15074..15805)
                     /locus_tag="PLD98_02995"
     CDS             complement(15074..15805)
                     /locus_tag="PLD98_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQA05250.1"
                     /translation="MKLAVQLYTLRRDFDSKEGFLALLPKVKAIGFDGVEFAGYHGLS
                     AEEIKKALDDAGLEAVGTHVGLDSLKPENLEETLKFHKTIGCKYVGIGGANTSTEENL
                     QNVLNIMGNAYNRAKEEGITIYFHNHTEEFHPVKGSANKALILDRIKEVCAIELDTYW
                     SFCAGVDNYNYILENKDKIVHLHIKDGVNRKPKALGEGENDLKAVFKAAKEAGFEWLI
                     LENDDPVPTGLEDIERSMNYFKANL"
     gene            complement(15840..>16129)
                     /locus_tag="PLD98_03000"
     CDS             complement(15840..>16129)
                     /locus_tag="PLD98_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05251.1"
                     /translation="INSGSGDYLGEIAKYGARIHHFHIKGTLNYGGKHIDDPPAGLDM
                     IQWRPIMGLLYKHAYNGMLSIEPHSRTWRGELGEWGVKVTIDYISKMVYGG"
BASE COUNT         3986 a         3906 c         3875 g         4362 t
ORIGIN      
        1 agtccgacaa atccgatttc ggcgacgccg cagtttttac cgtagattcc ggagagtcca
       61 tgcttaaaaa ggcagaatcg gtgggagttc tctccactga ggacgaggac atccgctctc
      121 ttaaagagat gattacttac ggtcttaagg gcatggctgc ctatacagag catgctttta
      181 acttaggcaa agaggacctt gaaatcatcg agttcatgta taataccctg gcagagcttc
      241 ttagggacga ccttacggcg gatgagcttg tcgctctgac tctcaaaaca ggcgaatacg
      301 gcgttaaggc catggctctg cttgatgagg caaatacctc aagatacggc aatcccgaaa
      361 tcacagaggt taatatcggc gtcagaaaga atcccgcaat tctcatttca ggtcatgacc
      421 tgactgatct ggagcagcta cttgagcaga ctcagggcac cggcgtggat gtatataccc
      481 acagcgagat gctgcccgcc cactattacc ccttctttaa aaagtacgag aatttcgccg
      541 gcaactacgg caacgcctgg tggaagcagg tcgacgagtt tggtcccttt aacggcccga
      601 ttctctttac cacaaactgc atagtcccgc caagaaaaga ggaaatcaga aagagaatct
      661 tcaccaccgg ctctgccggc ttccccggct gcaagcacat tgaggcggac gaaaacggca
      721 aaaaggactt ttccgaaatc atagagcttg caaaaactct ccctgctccc gaggaaatcg
      781 aaaccggcaa gataatcggc ggctttgccc acaatcaggt tttcgccctt gccgacaaag
      841 ttgtggatgc ggtaaagtcg ggagccatta ggaaattctt tgtaatggcg ggctgcgacg
      901 gcaggatgaa atcaagggca tactacaccg agtttgccga aaagctcccc aaagacactg
      961 taattcttac cgccggctgc gcaaagtacc gttacaataa gctgccgctt ggcgacatcg
     1021 gcggaatccc cagagtcctt gacgctggtc agtgcaacga ctcctattcc cttgccctta
     1081 tcgcccttaa actcaaggag attttcgggc tcagcgatat aaacgagctg ccgctcgcct
     1141 tcaatatcgc atggtatgaa cagaaagcgg ttatcgtcct ccttgccctt ttatatctgg
     1201 gcgttaagaa tatccacctc ggacccacgc tccccggatt cctttctccc aatgtggcaa
     1261 aggtccttgt ggaaaagttc ggcattgcgg gcattgatac cgtagactcc gatatagaaa
     1321 tgttcatggc tcagtaaaac aaaaggcact tctaaaagga agtgccttta atgtttgcgg
     1381 gggtttattt ctgcttttcg ccgaagccta aatcaggaaa aagggctgtt caacagcgga
     1441 aaaatattat atattaaatg taaatatttg taagtagaag agagtatgac taccggttta
     1501 aggtttatgg taggtcccga tgagaaaata ctgtatcaag gcaagcccga tttaaaatgc
     1561 tttgttttcg aaaccatatt caacccctca ttgcccatag tgattctctg gggagccctt
     1621 gacctttttg tgctcggtaa tctcattttt gcgaaaggca gcggaaagtc tttctttgtg
     1681 ggctttattc ttatccatat gacccctgtc tggatttatc tcagcagcat aatctttatg
     1741 gcaagaaagt acagaaacat cgaatacata gtcaccgaca aagcgattta tgtatcgggg
     1801 ggagtgttca gccgcacata taggtcaaag cccttcgcgg agctgtcgca cattgatttg
     1861 cacaggagcg tttttgacca gcttttcaat gtgggagatg tgatagcaaa cacctcgcac
     1921 atccgcccga accagaagag cgcgactatt acacttaaca gcattttaaa ttttacggaa
     1981 gtctataatc tgataaaaaa gctccagcag gatatctata cggacattat gttccccaat
     2041 gagtacagac ccgatgtaaa ccgcggctac aaaaccgaat acaaaggctt tgaataaaaa
     2101 aagaccacac agaaacgctg tgtggtctta ttattaaata ttgaactgac ggataaggcc
     2161 ctgggtcatt acatccgcca ttttcattgc ctggttctga atttcgtcat aaatcgtaac
     2221 gccttttctg tattgtcctc tcagaatgaa aacagcctcg tcctccgtca tgcgcagatg
     2281 gtcaaacaga aggcatctcc actggagttt tccccagtat gggttgatgg acgcaaggaa
     2341 tttcgcgata tcctcagcgt tttcatacca tttgcagcgg attttgtccg cttttataac
     2401 atttctttcc ttcagagcgt ttacaagctc ggccgcgatt agcagatggt cttttaaaag
     2461 ctttttaagc tgctgagcct ttgcctcgcc gtagaatctg cagaaaagct tcgcgaaatc
     2521 acaggggttt tgaagcagcc tgtcggtcac cgcctgaagg tcgggaaggt cgaaagcggt
     2581 gctttcaata aaggagcgag tccaaagcac atgttcattc cacagctcac gcagcttgtt
     2641 cgataagtct atttgcttgc aggtaatttc tctgtcaaaa gctgtttgat tcattcttta
     2701 ttccctccgt aatttattgc agcagaaagc atctatttta ttttatgttg cgcccccaag
     2761 agtgtgagga attaataaaa aaggccccag cccttatggg ctgagacccg attctatgct
     2821 ttatacaagc tgtgcgaaga caacattgtc aacttcaata aagccctcgg aatctgtctt
     2881 ggagaacaga atccttgctc ttacggttcc tgcgggtgca ggctcggtta cgccggcgaa
     2941 gggtatccac tcaatgaagg tgttggtatc cgtgggcagt accctgttgg aaacgagact
     3001 gagacctacg cccagctctc tgtcaaatct gtcgagccag atgactttca ttatcattgt
     3061 gccgaagtcg gctgtttcaa tggtttttct aaatccgaga cccgtgctga acagatagca
     3121 cttgccctcg gggtcataaa ggtcaacatc ctgccacaaa gcgccgccaa tctgacctat
     3181 gcctgcgtcg gcagtgcctt cgtaaacatc gtttcgctca atgagattta caaggctctg
     3241 ctgccagtag ttaaggccgt cctcaaagct gggattgttt acaaggttct gagtgagaac
     3301 aggagcgaaa atcacattgt caagccgcag gaagtctgcg gtggtgggta ctgttgcgta
     3361 gaaggtaagc cttgcggttg cggtgccggg taccatcggg tagctgacga aaaggtagct
     3421 caggtagttc tgctgattat tgagagtttc gttcgggata gatacattaa gacctgagcc
     3481 gatgggattg tcgttatagt caagccactc aatgcgcacg gtaagaccta cattgccggt
     3541 accctgagcc gcgaagctga gaaggaatga ggtctgcggc ggcaggtggc ctacgcttat
     3601 gtcctgagaa agatttccgt tgacctgggt cagagcgtgt cccgcgccct caagggactc
     3661 tttgtaggag gagaggaatg cggtgtcgcc gcccgggttc gctacccact caaagaggtt
     3721 tgcctcaaag tctccgttcc tgataaggtc ggtgtaagcc atcggagcca gaagcacacc
     3781 gtctatgaag atgaaatccg cttcgctcat aaaggaaggc gaacctttgc tgaacgagat
     3841 tttcgcaaag gccgtgtcct caggcggtct gtcggtctgg gcaaagaagg taatcttcgc
     3901 ggtgctgttg attctgccgc cggggattac cattctgagg ccgaggccaa tctggttgta
     3961 attgctgtca agccatgcga cttccgcaat gagaattccc agggtcagct cctgctgaac
     4021 tcccaaaagc ggcgagccca cagcgttaaa gctgaacagc atcgggcttc tgcatacgcc
     4081 ctcaagggat acaatctgat agagggaagc aactccggga ccgagtcttg cttgaatcgc
     4141 tccttcgtaa acatcggtat ttgtaaaggt tacatttgaa gcaacccacg gaccgtactg
     4201 gctttcaaag ctgaaatcct gcaccaaatt gccgcagcgt ttgcatttgg gctttttctt
     4261 atcgtgcttg caatcgtccc gcctgtcggg ctctgcagaa tgattgcagc actcgggata
     4321 tggtctgatt gcgttattat aagacatatg cattatctcc ttgaatttag taatgatgtt
     4381 ttaattcaac atcccatagt attttatgtt tgagcccctt gcagtgggac aaatactttc
     4441 gatgtttggg gacaaactta aagcttccaa agacaaaaga attgatttat tccccgcaaa
     4501 aagttattat ggttttggaa aataaggagg ctgttatgaa catagttgat ttaaagcagt
     4561 atataaagcc gggcaagctt tataaggaga aggagagcac attttacgct caggctcaga
     4621 ttccgaccga gtgccgctca aaccacgcaa gctgccttac tcagcttgac aacggggata
     4681 ttctctgcgt gtggttcggc ggctccgccg agggttcggg agatacgaaa attcacctct
     4741 caagactgaa caaaggcgca tcccagtgga ctgagcctaa gagaataagc gatgactata
     4801 actgctccga gcagaacccc tttatctacg atttcggcgg cgggcattta tggcttgtcc
     4861 acaccgctca gaaaacaagg ggaatgtcaa aggaagaatg gaacgccctt gtaaaaagcg
     4921 gcaaggcaaa gggctcatac acaatgcagg aaacagccga aatccgcata ctggagtcct
     4981 ttgactacgg ctataccttt aaaaagagag agcccttaaa ccaaaaaagc ggagccttct
     5041 gccgccagcc gataataagg ctctcaaacg gcgatttgct aatgggtatg tggtacagcg
     5101 tcgtaaggga aggggatttt gaaaggggag agacctacgg cggggattat tccgtctgcc
     5161 ttatctccga ggacaacgga gcgacttgga aggagtatcc cgttcccgac agcgtgggca
     5221 gagttcatat gcagcccata gagcttgaaa acggcaggct catagccctt ttcagaagcc
     5281 gctttgccga caggatatat aaaagctatt ctgaagacta cggcagaaca tggactgccc
     5341 ccgttcccac ggttctcccc agcaacaact cctcaattca ggcaaagaaa cttcaaagcg
     5401 gcaacatagc ccttgtctac aaccacaccc cctatattgc gaacgacgcc tcaaaggtgc
     5461 gctggcccgc gaactcccgc catcatcttg cggtcgcgct ctccgaggac ggcggcgaaa
     5521 ctttcccgta catcagagtc attgagcacg gcgaggagtt tttcggggag aattggcgca
     5581 agaacaggga atgttcctac ccctcaataa ttgaggcaga tgacggaaag cttcatgttt
     5641 cctataccta taacggcaga cgctgcataa agcatgtaat aatcgatgaa aactggatta
     5701 agggggaata gcgtggcaac cttaaagggc atagtccccg cattaataac tcccgtaaca
     5761 cacgatgaga aaataaacga aaaggcatta agaaagcttg caaacagact gattgacgcg
     5821 ggagtccacg gtctttttgc ccttgggacg acaggggagt tcttctcctt gagccaaaaa
     5881 gaaaagctca ccgccatatc gactgtaatc gacgaggcgg cgggcaaggt acccgttgta
     5941 atcggcacgg gagcggtcag cacaagggag agtatagagc tctcaaaaaa agctgaggag
     6001 atgggagcgg actatctctc ggtcataacc ccctatttca actgcctcag ccaaagggaa
     6061 ttaagtgagc actatctgag aatagcctcc gcggtgtcaa tccccataat cctatacaac
     6121 atccccgcca gaacgggact gagcctttct gacgacacag tctcagagct ggcaagggtg
     6181 ccgaatatag tcggcataaa ggacagcagc ggcaaatttg acaatatact gtcttacatt
     6241 gaagtttcaa aggggcttaa tttctcggtc tatgccggaa cggactcgct gatacttaaa
     6301 acccttatgg cgggcggcag cggcgcggtt gcagcgacag cctgcgtttt ccccgagctt
     6361 gtggtgtcaa tctatgagtt ttggaaaagg ggcgacatgg aaaaagccga aaaggctcag
     6421 gaaatgctct ttgccattcg ggatttcagc gcaaaattca gcatccccgc cgcatacaaa
     6481 gcggcgctgg agattcaagg catcgatgcc ggcccgccca tttcacccgc cctgcaactt
     6541 ccctatgaag aaaaggaaaa actaaaaaac cttctaaaaa aatacgaaaa ggaaatctca
     6601 gaactgctat cataaaaaag cgctcccaag cggagctctt ttttattaat taaaagtttg
     6661 ttcactcaag tttttttgaa ctgtgctata atttgcccgt tgtatttcag tggctttgga
     6721 ggaaaaatgg tttcacttct gcttgcgata atatatttgg cttttataag tctcggattg
     6781 cccgactccc ttttgggctc agcttggccc gtaatgtatg agcagttcgg cgtgccgctg
     6841 tcctacgcgg gaattatttc aatgataatt tcgggcggca ccattatatc gagccttatg
     6901 tcggacagac tcacaagaaa attcggcgcg ggactcgtca cagcggtaag cacgcttctc
     6961 accgcccttg cccttttcgg attttccctt tcccgctcat acattcccct ttgcgttttg
     7021 gctgttcctt acggtctcgg cgcgggagcc attgacgcgg ctttaaataa ctatgtagcc
     7081 ctgcactacg cctcccgcca tatgagctgg ctgcactgct tttggggagt gggagcggcg
     7141 ataagtccct atattatggg ctacagtctg agtaaagatc taaactggag cctgggctac
     7201 agaatagtgt ctataattca aatctgcttt acggcagtgc tctttatcag cctgcccctt
     7261 tggagaaaga acagagagaa cggacatacc acagaaaact cccgcccctt gactctcaaa
     7321 gaagcggtca gcataaaggg ggtaaaactc gttcttgctg ctttcttctc ctattgcgcc
     7381 cttgagtgga ccgcctgcct ttgggcaagt tcttatcttg ttgagtacag gcagatagac
     7441 gccaacaccg ccgcaagatt tgcctcgctc ctgtttatag gcatcaccgt cggacgcttc
     7501 ctctgcggct ttatcgccga caaggtgggc gacaaaaacc taatccgcat aggaacggca
     7561 gtaaccatgt tcggcatagt tcttgtagcc ctgcctctta aagtggattc aatcgccctt
     7621 gcgggtctta ttataatagg tttcggctgc gcgcccattt acccgtccat tatccattct
     7681 acccccgtaa acttcggcaa ggaaaactcc caggcaatcg tgggcataca gatggcaagc
     7741 gcatatgtgg gcagcacctt catgcccccc gtattcggaa tgcttgccga caatatcagc
     7801 atggggcttt atcccttcta ccttatgttc ttcggagtgc tgatggttat tatgtccgaa
     7861 cggcttaata aaacggtagc taaagcataa attgatcccc cttttatagg gggatttttt
     7921 atactatata ggctatcggc acaccgttta aatgcttaga gagttcattt ctcaaaaact
     7981 cctctccctc atggcgcaga gattctttgt accagtactt gcccttgccg cgccccgtca
     8041 tcttcacctt gtcaaaaagc tcaatcgcct tcgggaaagc ctcattattg atggagcggt
     8101 gaataaagct gtatgtcata aagatgattt ccagtttgat tttccgcttt gccttttccg
     8161 tcagctcatc ggcgagcctt tctataagct ccgaatacag ccttttccaa tcgcttacaa
     8221 aaacgacggg ggcgattaaa agcccaattg gataatccgc ctcgcaaagg gagttcaccg
     8281 cctttattct gccgctgagc tgagaggtgc cgaattcgat ttttcttata atctcctcag
     8341 ggtttacgct cattctcaca attgttctgc ctttgtgctc caaggggagt atcggctcta
     8401 tcatgtcgaa ttttgtggga aaggttataa aacccttttg agctttcgcg aaattctcta
     8461 tagtccaaac gagatttccc gttattgtgt tctccagcaa caaatcgctg ttgctgccga
     8521 tttcaaaaac aaggtccctg tccgcttttt cggcttcttt caggagctta aacatcattt
     8581 cctccctgtt tacaaagagc ctgagataag agcatttatt gtagttgcag acaagatagc
     8641 agtaaaggca catggcgctg cagcccgatg aggtgtaggg caccaaaaaa tccgaagttt
     8701 tgttgttcgg aacatgcttt aaagattttc tgacgcctat tatcagatgc cttttcatcc
     8761 tggcaaattc tttgttttcg ttttttctca gttcctcaat gttattgtgg ctttcaatcg
     8821 gaatccacgg cacatccttg tacttttctc taagttcctt gccgagctta tattcaagag
     8881 cttttggctc gaagtaaacc ttatcgggaa acaaatccac atccccttta tattttttat
     8941 tagtatttca ccgcctttga gtttaagcaa agcaaaacat taaacaaatt gacaattttt
     9001 ctcagattgt ttatagtcaa agaggaaata aaaaacggaa ggagtaaaaa cgatggcaaa
     9061 aaaactatgc gctctgctgc ttgttgtttc aatgcttttt accattgcgg ctacgggtct
     9121 tgccgctgac gctaaaccct ttgaggacag cgaattcttt gaaataggcg attattccat
     9181 acattacaga ttcaagcccg cgactggcga gtttaagggc aggtttgtgc tgattcacgg
     9241 acttatgtcc tccaccaccg cgtgggagcc cttcgccgaa attatgaccg ccgagggcta
     9301 tgactgcctg cttgttgacc tgccgaactt cggctacagc acaagggaaa atgtaaaggt
     9361 tcaggcaatc ccgagagagg atattcttgc agctcttatg gaggaactgg cacccggaga
     9421 gaaatggttt gtgggcggcc attcaatggg cggcggagtc gccatggttc ttgcctccaa
     9481 gcaccccgat ttgattgagg ctctgttcct ctatgctccg gggcttaata caaaggctat
     9541 gttccccaac aattcggaaa tgtcccttca gattattggc tcaatcttca acgcattttt
     9601 caagatgctt gtaagctttg ccagaatacc ctttgtaagc cagatgataa agaaaaatgt
     9661 tggctatgcg cccgactttg atttgaacag aaggtatctt gacgctctga gcgttgagaa
     9721 tacgggaatg ggacttatct ttatgatgaa gagggcaaca ccccttgatg tggaggctct
     9781 gcatgagctg aatatgccca ttttcctctg ccttgccgag gacgacaaaa tcgttcccgt
     9841 tgacggtgag atggcaatca aaattgcgga caatctgccc gagcataccg taaagctcat
     9901 tatgccgggt cttaaccact ccttcattga aagcgacgct gacagggtca cagccgcaac
     9961 ccttgacttt atagcaggtc ttgcggcata atgagaaaac gcaacagggc tcacctttct
    10021 ttcggtgagc cctgttttcg tgcaggaggc gggagattat ttgaaaaagt cgatgtagag
    10081 tacttctgcg ctgtctatat ccatagccca tattccccac atggggtcaa tggcgtagag
    10141 tgtgctgcct atatatgtgc ctctcagctc atcgattccg ggagcggggg agtaggtgag
    10201 cttaggcact atccttctgt tctcaacgga gaaggagcgg agaaccgaaa gctgacctgc
    10261 ggatgtttgt ctatactgca tcacagtgac cacatacaga ttcttttcgg gtatggtaag
    10321 gaatgccttg tggttggagg atattcttgc gctgggatag gtgatatggt ctatttcgat
    10381 aggtttagtc gggtcgctaa catcaaagag ggaaatttta gcgtctccgt taatgcccgt
    10441 atccgtgccg tcaagaccta tgcctataag gagattatcg cccacggggt ggagatactc
    10501 ggagaagccg gggattttaa gctcgcccac aatcttggga tttctagggt cgtgcaaatc
    10561 aaacacgaac agcgggtctg taagaacaaa ggttaccaca taagcggtat cgcccataaa
    10621 gcgcacggat tttatccgct cgcccttggc aaatcctgtt accttgccca cttcattcaa
    10681 attcttgtca agcacaaaca ggttgttgcc ccgtgagtcg ccggtggcta ttctgaaata
    10741 accgtctttt tcgtccatgg cgaactggtt gagaagccct ccgctgacgg tgtttctgga
    10801 attgagcctt atattgccgt cctttaaggt aaagtagaga atctctgtct cgtccgcgtc
    10861 ggtgaaaacc atatcgcttc tgaattcgaa ggttctgacg ccgccgtcat atatggagcg
    10921 ggcggtataa aggttttcgt tcctttgata tatgctgtca gcgccgccca gaaccgcctt
    10981 tgtgtctatc tttgagttct tctcaagact cttgataagt cccaccacaa ggtagctctg
    11041 gtaatccgac ttttcgggga tatagatgct ttcgggagct aggcggtccg ctttgccttg
    11101 aatctcgatt acgggaatcg agattttctt tgtaacctct tcctcacctt cgatatagta
    11161 aacatcgtac gaggttacaa ggcttaatga gccgttataa attctagacg atacatagta
    11221 gccgtcctgg gtgtagtgga gaagctcctt ggggctgctt ctgtcgctga tgtcaaatac
    11281 ggtaacgccc gtaaccgatt tgccctgcct gattgtatca tttacgcgca tgggtctgta
    11341 atagtcgaaa atggcagcga gcctgttgcc ttggacaaaa atctccaaaa tcctatggtt
    11401 ttgaggtctt gtgaagtatt cgtcggcaac tgtggtactc ttttcgaatg aggtgaaagc
    11461 ttcctttgtt atttgtccaa gcctaatgaa agaagtgtgc ttaagagtat tgtttgccgt
    11521 gtccacaatt cgaacgccgt actgagtggc aatataaagg tatctgccgt cattctttat
    11581 gatatccgct tcggcaacgc cctcaacctg ctcgttggtc ctgccgtagc cgacgctctc
    11641 cgatccggag ggagtgtcaa gccaattatc ttctcttact atgtccccga cttttttgaa
    11701 gctgaatagc cctccgaaga aattctttaa ggagaattta gaagaatttt gcgccttctt
    11761 ccttgccttc caaatgcttt cttcaatctc ggagtaagaa tttgccttct ttacgccctt
    11821 aacttctttt tcgttattct cctgtgttac cgaaacggag ctgagatttg tatcggcggg
    11881 aacatttccg attttgtcgc tctctttaaa gtcgaatgaa ctgctgccta tatcgcctga
    11941 gagaccggct tctgtttcgg ctgcggtttc ctgaatctcg gaggggggac ggtcgagacc
    12001 tatatagaaa acggctgcag ccgcgataat cacaaaagca gcggcggcgg caatgactct
    12061 cttggaagga gcccaggcca cataaatgcc cttctttact tctttttcat ttatttcggg
    12121 ggcttttgag ttttccttta aaagctgagc tatgctttcc ggctttaaag tctcgggaag
    12181 ctcgaatttc tcagtatcct ttttaagctc ttcggaaatc cgttttaaaa aagccttatc
    12241 ccttttgttc atattaacaa cccctcaaag accgtagttt aattgatctc tcatcttttt
    12301 aagtgcacgg gcaagccgag agcgaaccgt tgaggacgga cagccaagca ttttggcgat
    12361 ttcatgactt ttgtagcctg ccagtgcact taaaaggaca atctcccttt cctcgtcctt
    12421 taatgtgtcg agaacctcca aaagctccga ttttaaggag gcggggagat actcatcgga
    12481 gcccagatgc tccgccgagg aaatgtccac aaaaacgggg gcggctgaat ttttcataag
    12541 tcttgtcttg cagcagtttg aaagtatttt aaaaagccaa gacttgaacg ccccttcgtt
    12601 tctaagggag ccgattttgc tgaatgccaa aagcacggcg tcttgaaccg cgtcctgagc
    12661 atccttttcg tttttcagat agcataaagc aaacctgtaa aggtcggcgg cgtaaaggga
    12721 gtagagctgc gcaaatgcgt cagcgtcgcc gctcattgct ttttgcacat tcgatttaac
    12781 atctgtcatc aaatcacctt gcttccgtcc tcacagataa gagattgaag agccgaaaat
    12841 tgttgcaggg ctttataatt ttctcaagtt tataatggat taaaaatttt ggcaagaaaa
    12901 aactgacgca cccggggtgc gccagtttga atttgtagtt tagattctgc acttgacaac
    12961 cgtgtttttc tctgcggatt caaataccgc ctcgattgcc ttcatggagc gcctgagctg
    13021 atcgtgagtt actatctgct gagcgccgtt ccttaataca tcgtaaatgt tgatgtagta
    13081 gtcggaccag tcggctgtga ccttcggcag tgggaactcc ttaatggttt cgcttgttct
    13141 cggtgccatg gtctttgtaa ttcccgcgcc tgccttaatc ggcaccgcgt caatcttctc
    13201 ccagtcctta atcttaacaa tcttgccgtt taaatcccag tcctcgataa cggctgtgcc
    13261 gttctcgcca aggatgtacc agcggggcag ggagatgaag ttgcttgtgg caacctccac
    13321 atagcataca agaccgtttt caaatgtaaa ggttacatag cagccgtcgt cgacttcctc
    13381 gtttgtgaca ttggagaggt ttgcgtgaac tgatacaacc gggttgtcaa ggttcagcat
    13441 aaggatctgg tcaaagagat gtacgcccca gtccagaacc attccgccgc cgtgagcctt
    13501 ttcggctctc cagtcgccgg ggatgcctct tgagccctga actcttgact cgattctgaa
    13561 aatcctgccc agagtgttgg actcaataat ctccttggct gtaaggaagt cgtcgtccca
    13621 tcttctgttc tgatgaactg tgaagagctt tccggtcctt tcgcttgcct caatcatttc
    13681 ctcaaggtcg gaaagtgaaa tggtaacagg cttttctgaa ataacattta cgcctgcttc
    13741 catggctcta atggcgatgg gtttgtggaa ctcgttgtaa gttgcgatgg taatgagctc
    13801 tacatcgtca gccaaaagct catcaaggga attgtaggca tgaattccgt tctgccttgc
    13861 aagctcgcat ctttcctctt tgatgtcgta aatgcccttt acattgtagt tcggcatctt
    13921 gagaagctcg cggacatggt gagcgcccat gccgccgtaa cccacaacag ctacattgat
    13981 tttatctggc atttctgaat agacctcttc tttccatatc gataagtttg cagagtgcat
    14041 attttcagcg gctcaaaacg ccgcggagtt tcggtttata cccaccacat ctcgccgggg
    14101 gtttcggtca ttattgcctc cttgagcgtc gcgacagcct ttgaaagacc ctcgttctgg
    14161 ctcataagag cgtcctcgtg ctcaatggag attgcatagt cgtagcccac aaggcgcaga
    14221 ttgctgatga tgtccttcca gtaaacaaga tcgttgccgt agccgacgga gcggaaggtc
    14281 caggagcggt tgagcacatc gccgtagccc tttgtgtcaa gcacgcccgt aacggctgtg
    14341 ttgtacttgt cgatcttggt gtcttttgcg tggaagtgga agattgcgtc tcccagcttt
    14401 ctgatgacgg ctacaggctc catgccctgc caaatgagat ggcttgggtc aaggtttgcg
    14461 ccgatttcgg ggcctactgc ctcacggagc ttaagcagtg aatcggtgtt gtaaaccgca
    14521 aagccggggt ggagctcaag agcgatcttg ttgacgccgt gctcctttgc gaaggcaaca
    14581 aggtctttcc agtaggggat aagaacctcg ttccactgat agtcaagaat ctcaagatag
    14641 tcgttcggcc aggggcaggt tacccagttg gggtactttg cgcccggatg gtcgccgggg
    14701 cagccggaga aggtgttaat ctgatgaatt cctacctttt cgcaaaggag gatggtcttt
    14761 ctgatgacct cgtcataata cttagctgtc tccttgtcgg ggtgaacagg gttgccgtgg
    14821 cagctgaacg cgctgatttc aacattgtac ttctttatgg tgtccataaa tttcttgaat
    14881 tcagcctcgt cattcagtag cacatcggga tttgcgtgag cgttgcccgg atagccgccc
    14941 gtgcctatct caaccatttc aatgccgaga gaggtaaaat atttcagagc ctcttcaagg
    15001 cttaaattgc tgagcagagt agtgaatact cccagcttca tattatcatc ctttctcttt
    15061 tttaaagcat tgattacaga tttgctttaa agtaattcat gcttctttca atgtcctcaa
    15121 gacctgtggg aacggggtcg tcgttttcga ggatgagcca ctcgaagcct gcttccttag
    15181 ctgccttgaa aacagccttc aggtcgtttt cgccctcgcc cagagccttc ggctttctgt
    15241 ttacgccatc tttgatatgg agatggacga ttttgtcctt gttctcgagt atgtaattgt
    15301 agttgtcaac gccggcgcag aagctccagt atgtgtcaag ctcaatggcg cagacttctt
    15361 tgattctgtc aaggatgaga gccttgttgg cggagccttt aacggggtgg aactcctctg
    15421 tgtgattgtg gaagtagatt gtaatgccct cttcctttgc tctgttgtag gcattgccca
    15481 ttatattcag gacattctga aggttctcct ctgtggaggt gtttgcgccg cctatgccta
    15541 catatttgca gccgattgtc ttgtggaatt taagggtttc ctcaagattc tcaggcttta
    15601 agctgtcaag acccacatgt gttccgacag cctcgagacc tgcgtcgtca agggctttct
    15661 tgatttcctc ggcagaaagt ccgtgatagc cggcaaactc cacgccgtca aatccgatag
    15721 cctttacctt ggggaggaga gccagaaagc cttcctttga atcaaagtca cgccttaatg
    15781 tataaagctg aacagcaagt ttcataactt ttactccttc tctttaagct gaacggaact
    15841 tagccgccgt aaaccatctt tgagatgtag tctatggtga cctttacgcc ccattcgccg
    15901 agctcgcctc tccaggtcct tgagtgaggc tctatgctga gcatgccgtt gtaagcgtgc
    15961 ttgtaaagca gtcccataat cggacgccac tgaatcatgt caagacctgc cggcgggtcg
    16021 tcaatgtgct tgccgccgta gttgagcgta cccttgatgt ggaagtgatg aattctggcg
    16081 ccgtactttg ctatctcgcc cagatagtcg ccgctgccgg aattgatgc
//
LOCUS       DAONVQ010000027        16057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2698_length_16057_cov_5.398825, whole genome shotgun sequence.
ACCESSION   DAONVQ010000027 DAONVQ010000000
VERSION     DAONVQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16057
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2698_length_16057_cov_5.398825"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            594..1319
                     /locus_tag="PLD98_03005"
     CDS             594..1319
                     /locus_tag="PLD98_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05252.1"
                     /translation="MKCPKCGEVFDDKTICPNCNEPLTDENIETAGKEPAEEVKPQAE
                     NASETKSEGESAESAAAETEAKPEQEIKPAKKAVKPAADKKHFNVKKTASGLITVFKN
                     LSPLKKAIACACAALAVFLIVFGIWYAAGVSSTKKTLMQDWVLVEQNNNVYIRTLVFE
                     KDKAEFCFESRFAWLNNTIAVYEYKVVFPSRVKFKTDGFSKTVKIDFSEDKQSFTITP
                     ALSGDDESQTFKAIEKDRRYIWK"
     gene            complement(1356..4100)
                     /locus_tag="PLD98_03010"
     CDS             complement(1356..4100)
                     /locus_tag="PLD98_03010"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HQA05253.1"
                     /translation="MSEILAKVKKSRFAEKCRENKYPIVGGIAAGIIMLVIYAAFRMK
                     PFGDITILRMDLYHQYGPLFAELYERIVNRMSLIYSWTSGLGSPFLGNYFNYLSSPIG
                     GIIMLLFGHKNMPEAIAAMILVKCVLSAAFFTYYLKKSVGKESYVTSAFGVLYAFCSY
                     FVAYYWNVMWLDAMALFPLVILGIERIINKGKPALFITSLAVIFLTNYYMAYMVCILS
                     VLYFIYYYLSNYTLGKRFLYSGFTFAVSGVASALIMAFALVPVFFILKESSATSGTFP
                     SEFKSYFKIFDFLANHLASVEPTIRSSGTDVLPNVYCGILTLILLPLYMLSKKISFRE
                     KVMSTVLLAVFYFSFNTNYLNYIWHGFHFPNDLPYRFSFAYSFILLLLAYKVFVRLYE
                     FSMKAVALSGAALLAFIVIVQKLGSKNVDETSIWLSIALTVLFVIIIALVKNPKYNRA
                     ALCMLVLIAVSTDMVIGQGTNFSMSQAKEHYVSDYDQFKQLKADLDKYDKTPFYRMEL
                     TNLRTRMDNSWYFYNGVSVFSSMAKQDLAKMQKKLGMFGNNINSFTYNMQTPVYNAMM
                     GLRYIVNNDDKIEMNPKLFERALVPSTQKFTAYKNFYDMPLAFGVSARVLDWSIENDD
                     PFKVQADFFRLATGVSDVFEYLMPTISDSSNMRDIADSSLQSGSFSYGKINSNEDGSF
                     TIEVQITKAQNVYVYFRCSGAEKYMISGSAFSKTRSFDREMIIDLGRHKAGDVINIEV
                     PITKNSSGVANFYAVGLDLAKFREGYERLQKNGTLNLTEFKETYLAGDINLSSNMLVY
                     TSIPYDKGWTVKVDGKALTEEEIYKVGGALLAFKLPAGAHRIEMSFSQQGLIAGTVIS
                     FFTILLIALFVFLIKKNRPKALQKLDSFYAEGAVKIEEFPDYSDEELMEYEREKEKAE
                     "
     gene            complement(4188..4757)
                     /locus_tag="PLD98_03015"
     CDS             complement(4188..4757)
                     /locus_tag="PLD98_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQA05254.1"
                     /translation="MTDRRIRKTRTNVFNALSSLLLSRESPDSISVTELCEKADISKS
                     TFYLHFMDIYDCREQWADEYVESLIDAAGGLLYEDILKDPKKFSLGLVLAADSSREIC
                     RRLSPENCEPKNAINIKKRLIDKVIEKNGFEPGVNLSEILNLTLLIGGIFDFYAYSPV
                     TASRQRIAEEVEKKIKAVISPASAETPEK"
     gene            complement(4914..5840)
                     /locus_tag="PLD98_03020"
     CDS             complement(4914..5840)
                     /locus_tag="PLD98_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQA05255.1"
                     /translation="MALTNLAKGWKIALIILAVVILIAAVLIIFNFRYYFAYKLSSEV
                     FNIAEVQRTPGQITAMSCNVRTLSPSDTGKRSWYYRADLLLQNIKEASPDIIGFQEVT
                     PVHYKFFQEKLIGYDSIITYRDDKPISEACPVFYRADAFELIDKDSFWLSETPDVMSK
                     SWNSACYRICSYAILKEKQSGKEFIVFNTHLDHVSEEARIKGIEVVLDRIKKFEGRPS
                     LLMGDFNAYEDSETYKMATEHFTDARYAAKLSSKAGTFHAYGQSESVIIDYIMVTPNA
                     FEVEEFRVIDKTYGGAYSSDHNQVFVKLKFAE"
     gene            complement(5953..6519)
                     /gene="pgsA"
                     /locus_tag="PLD98_03025"
     CDS             complement(5953..6519)
                     /gene="pgsA"
                     /locus_tag="PLD98_03025"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HQA05256.1"
                     /translation="MNLPNKLTIIRMLITPIFLAVVLWDKLPYRFFIAAVIFIIGSVT
                     DFLDGRIARKHNLITNFGKLADPLADKMLTTAALLVFMQHGLCSIWVIMIVLAREFAV
                     TSVRLVASSQGVVIPANQWGKIKTISQMVFSIAIMILAGFNEMGSLPASFDFPLLSNI
                     LLWITAVLTIISGGTYLWASRSQIDYSK"
     gene            complement(7316..8647)
                     /gene="rimO"
                     /locus_tag="PLD98_03030"
     CDS             complement(7316..8647)
                     /gene="rimO"
                     /locus_tag="PLD98_03030"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQA05257.1"
                     /translation="MGIRVGFISLGCPKNRVDSEMMLARLNNAGFEIIDEIDYADIII
                     INTCAFIEDAKKEAIENILEMAQLKESGLVRKIIVTGCLAERYQEEILKEMPEVDGVI
                     GLGGNGDIVELCKRVLEGETVSVKPEKTAMPLEGERLLTTPGFWAYLKVADGCSNNCT
                     YCSIPQIRGPFRSRPMEGIIKEAKELAEKGVRELMLIAQDTTRYGEDIYGKKMLPELL
                     RELVKIDELKWIRILYCYPDRITDELLETMASNEKILHYIDLPLQHAHKEVLRRMNRR
                     GDPESLLALIEKIRSYMPDAVIRTTFITGFPGEGEEEFKTLRDFIEKAKFDRLGCFAY
                     SAEEGTFAATFDNQVDEQTKAERAEQIMLSQFKIFEEKQKALIGTVQEVVTEGYDAYT
                     DSYYGRTWRDAPEIDSAIKFISPYEHDEGEIVKVEVTAVEGYDLIGEIFED"
     gene            complement(8657..9274)
                     /locus_tag="PLD98_03035"
     CDS             complement(8657..9274)
                     /locus_tag="PLD98_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HQA05258.1"
                     /translation="MRFSIERLKGGKVRLLADGEQFAIVTEGFLRDLKLKAGDEITVE
                     DIDSIINEKTKAAALNKAMRLITLRMHSRGELVQKLKREFDGEAAEYAADELENMGLI
                     NDAEFSSLFGEELFERKGFARARIIDELKSRGVSSDTARAAVEFISDEDEFKRLIVLI
                     EDSFSANLSDEKERRRVINNLIRRGYKLYDIERALGSLQTDTEYF"
     gene            complement(9274..10401)
                     /gene="recA"
                     /locus_tag="PLD98_03040"
     CDS             complement(9274..10401)
                     /gene="recA"
                     /locus_tag="PLD98_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQA05259.1"
                     /translation="MANEKATDRKKALETALAQIEKQFGKGAIMRLGESTAMNIEAIP
                     TGSLTLDAATGIGGLPRGRIIEIYGPESSGKTTLALHVIAEAQKAGGEAAFVDAEHAL
                     DPVYAANLGVDVDSLLVSQPDNGEQALEITEALTRSGAIDVVVVDSVAALVPRSEIEG
                     SMGDSHVGLHARLMSQALRKLAGAISRSNTIVIFINQLREKVGVVYGNPEVTTGGRAL
                     KFYASMRIEVRKAEQIKSAATGGEIIGSRTKVKIVKNKVSPPFKEAEFDIMYGKGISR
                     EGEILDVGVKYGIIQKSGAWFSYNDTRLGQGRDNVKLFFEQNPEMTAEIEAKIREKLK
                     EESVSKRNVSSVIQETAEVQPETEKKSVAANRAILDITVDD"
     gene            complement(10546..12087)
                     /locus_tag="PLD98_03045"
     CDS             complement(10546..12087)
                     /locus_tag="PLD98_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQA05260.1"
                     /translation="MRRTEKTKAPKQKKSKKKLSVSALLFRSIASVFIIGIAIVVIAD
                     SRMINYVYLLKTGFDLWTSADIIQSAYSDPDRFYNTLREVELNNDISIEIYGPTGNLV
                     YSTSYSGPYIQPPFKKDDKISDEHKRNYVIVDSGIQNRGFHFDLREDTSGTVKREYLV
                     FVRDFSDGRLELFKLKNEIDDVARVGIIFISTLSTIMVTLMLFIVARLIKRFTDPLKE
                     MCRITDNMSKLDFSQKVGEMRGEEIDRLGKSINALSDSLNEALTDLKAKNQKLQDDIE
                     KEHTLDHLRQIFITGVSHELKTPIAIIQGYAEGLSVFIKEDPKMALEYADIIRSEAER
                     MNDMIMKLLEIIKYDSGEYSPNREVFNVFELVEDWFVRSKAQLKEKGVTVVNAIPTDA
                     LGYGDVIILGSVVNNYLSNAMSHVEDMGEGKNIKAFVEDMGEIWRVSIYNTGKHIADK
                     DIGKIWTSFYRADKAMSRAAGRSGLGLALVAAIQRLHRMEYGVQNVSGGVKFWFDVKK
                     PENTD"
     gene            complement(12065..12772)
                     /locus_tag="PLD98_03050"
     CDS             complement(12065..12772)
                     /locus_tag="PLD98_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQA05261.1"
                     /translation="MKKILIADDEDLIRRLVVDFFKKDGYEMVEAADGQEALDLFDKF
                     ESDFSAVILDIMMPNVDGWEVCRRIRKRSNVPVLMLTARSQEFDELTAFEAGADDYVT
                     KPFSPSVLVKRIEALIRRNERAAATSESVYSIDGLTIDDTAHEVWLNGKSVTLTLKEY
                     SVLFKLVSNAGKVFSRESLLDDIWGYDFYGDIRTVDSHMARLRTKLGDWGAAHIKTIY
                     GAGYKLEVEPNEKNGED"
     gene            complement(12826..13350)
                     /locus_tag="PLD98_03055"
     CDS             complement(12826..13350)
                     /locus_tag="PLD98_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HQA05262.1"
                     /translation="MKSKNGITYIAQAGVIAALYVALTYISAAFNLAYGPFQIRLSEM
                     LTILPVFTPAAIPGLVIGCVIGNLGSPLGIVDIIFGSVATLIAAILTRSFKPYKIKGL
                     PVLSALPPIIVNALVIGLELYIMLPAYAGLIGFAYGVLSVAAGQLIACLGLGLVLYVA
                     IEKSGVKKSLHFES"
     gene            complement(14397..15248)
                     /locus_tag="PLD98_03060"
     CDS             complement(14397..15248)
                     /locus_tag="PLD98_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQA05263.1"
                     /translation="MKSLTDSFVMHNGVKIPCVGFGTWQIPEGETVINSAKKALEAGY
                     RHIDTAAIYRNEVGVGTAVKESGIPREEIFITSKLWNSDQGYDTTLKAFDATMERLGL
                     EYLDLYLIHWPIPRDFRDSWQTKIKESWRAMEKLYKEGRIKAIGVSNFLIHHFEALLE
                     DAEIVPMVNQVEIHPGFNQSELVQYCHSHGVVVEAWSPLGSGKIMADPTLKEVADKYG
                     RTPAQVTLRWLLQRDILPLPRSTNPEHIAENARIFDFELSDEDMGRISSISAYGRLGG
                     DPDNINF"
     gene            15526..>16057
                     /locus_tag="PLD98_03065"
     CDS             15526..>16057
                     /locus_tag="PLD98_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HQA05264.1"
                     /translation="MDDPDPGGTFSILSAAALPELLQAEPVSSDGILLKLAILFFLIL
                     INAFFAMSEIAIVSLNDAKLEKLASQGHKKSMQILALTQNSAVFLSTIQIGVTLAGFL
                     TSSSAAQSFAPMLVSLIDPYLPDAVPRGLLLGVSTVIITLITSYFSLVLGELVPKKIA
                     LQKPEQVSYFAIGLLTF"
BASE COUNT         3791 a         4101 c         4020 g         4145 t
ORIGIN      
        1 ggggtgaggc aattttcacc gcaggcggcg gggcgttcgt ctgacgaaaa tcctgggcgg
       61 gcgcaaccat ctcggcgggg tgaccacttc cctcggcggc ggggcgggtt cgctcggtga
      121 agggtcggga ctcccctcaa cggcggctcg gcggcgggca agcggcggca ctccccctct
      181 gcggcggctc ggctggtagg cgaagggcag ggcagacggg cgtggttctg cgggtggtaa
      241 aacacctctt tgcgagggcg gcgggggtaa ccctctcggc gcggcggtgc ggagcgggtt
      301 gggaggtgtc cctaaaatgg ctcacccgcc tgtgtgtaag gggtacactc tccccatcgg
      361 gcgtgtccct catttagcca acccctcccc gctggggcgg ggctggctct ctaagcgggg
      421 cggttgccct cccacaaaaa aacgccctgc ctttgacggg ccgggcggcg gacattcccc
      481 ctccaacggg gcgagctgtc ttattttgac agaaattaag actaatattg aagtggcggg
      541 agtaatatgg taatattttt atgctgttca gaaaaaagca aaggggaatt aaaatgaaat
      601 gtcctaaatg cggggaagtt tttgacgata aaactatatg ccccaattgc aatgagcctt
      661 tgacagatga aaatatagaa accgcaggaa aagagcctgc cgaggaagtt aagccgcaag
      721 ccgaaaacgc ctccgaaact aagagcgaag gcgaaagcgc agagagcgcc gccgcggaaa
      781 ccgaggcaaa gcccgaacaa gaaataaaac ccgcaaagaa ggcggtaaag cccgccgcgg
      841 acaaaaagca ctttaatgta aagaaaaccg cctccgggct tatcaccgtt ttcaaaaacc
      901 tctcgccctt aaagaaagca atcgcctgcg cttgcgccgc gctggcagtt ttccttatag
      961 tcttcggcat ctggtacgcc gcgggcgtgt cgagcaccaa aaagacgctg atgcaggact
     1021 gggttctggt cgagcagaac aacaatgtct atatcaggac tttagttttt gaaaaagaca
     1081 aagccgagtt ctgctttgaa agccgctttg cgtggcttaa caacaccata gccgtttatg
     1141 aatacaaggt tgttttcccc tcaagggtca aatttaaaac cgatggattt tcaaagacgg
     1201 tgaagataga tttcagcgag gacaagcaaa gctttaccat aacccccgcc cttagcggcg
     1261 acgacgaatc ccagaccttt aaggcgatag aaaaagacag aagatatatc tggaaataaa
     1321 ttaaaagcac tttggatttc tccaaagtgc tttttttact cagctttttc cttttccctt
     1381 tcgtattcca taagctcctc atctgagtag tcggggaact cctctatctt gaccgctcct
     1441 tcagcgtaga agctgtccag cttctgaagc gccttcgggc ggtttttctt tataaggaac
     1501 acaaagaggg caataaggag aattgtaaag aaggaaatta ccgttcccgc gatgagtccc
     1561 tgctggctga agctcatttc aattctgtgc gcgcccgcgg ggagcttgaa ggcaagcagc
     1621 gcgccgccga ccttatagat ttcctcctct gtcagagcct tgccgtcaac ctttactgtc
     1681 cagcccttgt cgtaggggat ggaggtatag accagcatat tggagctgag gtttatatcg
     1741 ccagccagat aggtctcctt aaactcggta agatttaaag tgccgttttt ctgaagcctc
     1801 tcatagccct cccggaactt ggcgaggtcc agacccacgg cgtagaagtt tgccacgccg
     1861 ctggagttct tggttatcgg cacctcgatg tttatgacat cgcccgcctt gtggcggcca
     1921 aggtcaataa tcatctctct gtcaaagctc cttgtctttg agaaggcgga gcctgaaatc
     1981 atatacttct cggctccgga gcacctaaaa tacacataaa cattctgagc ctttgtgatt
     2041 tgaacctcaa tggtaaagga gccgtcctca ttggagttaa tcttgccgta ggagaagctg
     2101 ccgctctgga gcgagctgtc ggcaatatcc ctcatgttgg atgagtcgct gattgtgggc
     2161 ataaggtact cgaacacatc ggaaacaccc gttgcgagtc tgaagaagtc ggcctgtact
     2221 ttgaacgggt cgtcgttttc tatgctccag tcaagcactc tggcgctgac gccgaaagct
     2281 aagggcatat cgtaaaaatt cttgtaagcc gtaaacttct gggtagaggg gacaagcgcc
     2341 ctttcaaaga gctttgggtt catctcaatc ttgtcgtcgt tgtttacgat gtatctgaga
     2401 cccatcatgg cgttgtaaac gggggtctgc atattgtagg taaagctgtt gatgttgttg
     2461 ccgaacattc ccagcttttt ctgcatcttt gcaaggtcct gctttgccat ggatgagaat
     2521 acggaaactc cgttgtagaa gtaccatgag ttgtccattc tggtcctgag gttagtcagc
     2581 tccattctgt agaatggcgt cttgtcgtac ttgtcaaggt cagcctttag ctgcttaaac
     2641 tggtcgtagt cggaaacata gtgctcttta gcctggctca tggagaagtt ggtgccctga
     2701 ccaattacca tatcggtcga aacggctatg agcaccagca tgcaaagggc cgctctgttg
     2761 tacttggggt tctttacaag ggctatgatg atgacgaaca gaacggtaag ggctatgctt
     2821 agccaaattg aggtttcgtc aacattcttg gagcccagtt tctgaacgat tacaatgaag
     2881 gccagcagcg ccgctcccga aagggcgaca gccttcatgg agaactcata gagccttaca
     2941 aataccttgt acgcaaggag aaggagtata aacgaatagg caaaggagaa gcggtacggc
     3001 aggtcgttgg ggaagtggaa tccgtgccag atgtagttca agtagttggt gttaaagctg
     3061 aaatagaaca ccgcaagtag aacggtggac attacctttt cgcggaagct gattttcttg
     3121 gagagcatat aaaggggcag aagaatcagc gtcaaaattc cgcagtagac attgggaagc
     3181 acatcggtgc cgctggagcg gattgtaggc tccacgcttg ccagatgatt tgcgaggaag
     3241 tcgaaaatct taaagtatga cttaaactcc gacgggaagg tacccgatgt ggcggagctt
     3301 tctttcagaa taaagaacac gggcacaagg gcaaacgcca ttataagagc ggaggcgacg
     3361 ccgctgacgg caaaggtaaa gcctgagtac aaaaagcgct tgcccaaggt gtagttggag
     3421 agatagtagt aaataaagta gagaaccgac agaatgcata ccatgtatgc catgtagtag
     3481 tttgtaagga agatgacagc caaagaagtt atgaacagcg cgggcttgcc cttgttgata
     3541 attctttcga tgcccagaat cacaaggggg aagagcgcca tggcgtcaag ccacattaca
     3601 ttccagtaat acgccacaaa gtaggagcag aaggcgtaaa gaacgccgaa cgcgcttgtt
     3661 acatagctct ctttacccac ggacttttta agataatagg taaagaaagc ggcgctgagg
     3721 acgcatttta caagtatcat tgcggcgata gcctcgggca tgttcttatg tccgaaaagc
     3781 agcataatta tgccgccgat ggggcttgaa agatagttaa agtaattgcc gaggaacggt
     3841 gagcccaaac cgcttgtcca ggagtaaatc agggacatgc ggtttacgat tctctcatac
     3901 agctcggcga aaagcgggcc gtactgatga taaaggtcca ttcttaaaat ggtgatgtca
     3961 ccgaagggct tcattctaaa agccgcgtag attacaagca ttatgatgcc cgcggcgata
     4021 ccgccgacaa tggggtactt gttttctctg cacttttctg caaagcggct ctttttcact
     4081 ttagctaaaa tctctgacat ttcaaactcc ttaactccta aagttcttgc aatatttgac
     4141 attttaacat ttttaacact caatttcaac cgattattag actgcggtca cttctcaggt
     4201 gtttcagcgg aggcagggga aattaccgct tttatctttt tttcaacctc ctcggcaatt
     4261 ctctgccttg aggcggtgac ggggctgtag gcgtaaaagt cgaaaattcc gccgattaaa
     4321 agggtaaggt ttaagatttc agagaggttt acccccggct caaagccgtt tttctctatt
     4381 accttgtcaa tgagcctctt ttttatattg atggcgtttt tcggctcgca gttttcgggg
     4441 gaaaggcggc ggcagatttc tctggaggaa tccgcggcta agacaagccc taaactaaac
     4501 tttttcgggt cctttaaaat atcctcatac aaaagaccgc cggcggcgtc gataaggctt
     4561 tccacatact catccgccca ctgctcacgg cagtcgtata tgtccatgaa atgaagatag
     4621 aatgtgcttt ttgaaatatc cgccttttcg caaagctccg ttacggatat gctgtcgggc
     4681 gattctctgc ttaatagaag tgagcttaaa gcgttaaaca cattggttct ggtttttctg
     4741 attctgcggt ccgtcacttt tttcctcctt tgcccataaa agtacaaata aattataatg
     4801 gtataataat agaaatacaa ggcggattgg taaagttttg aactaaatct tgtaatctat
     4861 attattggca aaggaaaaaa gaatataaaa gggcgagaaa cctcgccctt aatttattcc
     4921 gcgaatttaa gctttacaaa tacctgattg tggtcgctgg agtacgctcc gccgtaggtt
     4981 ttgtctatga ccctgaactc ctcaacctca aaggcgttcg gagttaccat tatgtagtca
     5041 ataatgaccg actcactttg accgtaggca tggaaggttc ccgctttgct tgagagcttt
     5101 gcggcgtagc gggcgtcggt aaagtgctcc gtcgccattt tgtaggtttc cgagtcctca
     5161 taggcgttaa agtcgcccat taagagggag ggtctgccct cgaacttctt aatcctgtcc
     5221 agaaccacct caatgccctt gatccttgcc tcctcgctga catggtcaag gtgggtgtta
     5281 aagactataa actccttgcc gctctgcttt tcctttaata tagcgtagga gcagatgcgg
     5341 tagcaggcgg aattccagct cttgctcatt acatcgggag tttcggacag ccagaaggag
     5401 tccttgtcaa taagctcaaa ggcgtcagcc ctgtaaaaga cggggcatgc ctccgaaatg
     5461 ggcttgtcat ccctgtatgt aatgattgag tcatagccta tgagtttctc ctgaaagaac
     5521 ttatagtgca cgggagttac ctcttggaat cctataatgt cgggagaggc ctccttaatg
     5581 ttttgcagca gcaaatcagc cctgtaatac cagcttcttt ttcccgtatc cgacggcgac
     5641 agggtcctaa cattacagct catagcggta atctgacccg gcgtcctttg tacctccgct
     5701 atgttaaaca cctccgaact cagcttgtaa gcgaaatagt acctaaagtt aaaaataatt
     5761 agaacggcgg caatcaaaat gacaaccgcg aggataataa gagcaatctt ccatcccttt
     5821 gcgagattgg tgagtgccat aacaccgcct cctaaacact ttaagatgag taaattatat
     5881 cataatatac tgcgttaaca agcgggaaat tacaatatat aacaaaaaaa agcggagctt
     5941 agctccgcca ttttattttg agtagtcaat ctgactgcgg cttgcccaaa gataagttcc
     6001 gcccgaaata atggtcagca ccgcggttat ccaaagaagg atatttgaaa gcagcgggaa
     6061 atcgaatgac gcaggcagcg aacccatctc attaaagccc gcaagaatca taatggctat
     6121 ggaaaagacc atctgggaaa tcgtcttgat tttgccccat tggttggcgg ggataaccac
     6181 gccctgggag gaggcgacca gcctgaccga tgtcacagcg aactcccttg ccagcactat
     6241 cataataacc cagatgctgc aaagcccgtg ctgcataaag accagcagcg cggcggtggt
     6301 gagcatcttg tccgccagcg ggtcggcgag cttgccgaag ttggttatta ggttgtgctt
     6361 tctggctatc ctgccgtcaa ggaaatcggt gaccgagccg attatgaaaa tcaccgcggc
     6421 gatgaaaaac ctgtaaggga gcttatccca aagcacaacc gccagaaata tgggggtgat
     6481 gagcattctt attattgtaa gcttgttggg taaattcatg tcagactccg ttttaagttt
     6541 tctatgtatt atacacctat attaaaggcg aaaacggcag aaaaagttaa aatagtctgt
     6601 gaacaagaat ataagatttg agaaaaattt cagttccgcg ggactgccaa accccaaaaa
     6661 gggcacgccg cgcggggctg ggcgagtgtt gttcgctgat ggcagcgcaa aaccgccgat
     6721 gggggcgggg tcgcccgatg tgggcagggg cagtccgtag tttggagcgg ggcaatctgc
     6781 ggtcgggata tgtgccgccg agcatatccg ctcccttgcc gcccgccgcc taaagagcac
     6841 cgacttaaaa acgccgcccg ccgatggggg cgggggtcgc ccgatgtggg gacggctcgc
     6901 ccgctgttgg gattgccgcc atgtctccgc tcgtcgtggg cgggggcgta acgccgtatc
     6961 gcccgtatgg gtcgccggct tgagcggggt gctgccgcca aaacaccgcc cgccgatgga
     7021 acggggtcgc ccgttgcggc ggagtgtgcc cgccgttctc ccgctgttgg ggagggggta
     7081 ctgtttcgcc cgacattgag ggggtacgcc cgctatcttg cccgcatggg gtactggtcg
     7141 cctttcgggg gcggggttgt ccgcaatatt accgcttatt agtagtatgc ccgctgacta
     7201 atagtttacg cagtttgcgg gtctcagcgg cagggcttgt ccgctccctt tacgcccgct
     7261 gtcgggcagg gcgcggggtt gaccgctgtc gggggtgtcc gcagcgatgg ggctgttagt
     7321 cctcgaaaat ttcgcctatt aggtcgtagc cttcaacggc ggttacctca acctttacaa
     7381 tttcgccctc gtcgtgctcg taaggcgaaa taaactttat agccgagtca atctcggggg
     7441 cgtcgcgcca tgtcctgccg tagtagctgt cggtgtaggc gtcgtagccc tcggtcacga
     7501 cctcctgaac ggtgccgata agagccttct gcttctcctc gaatatctta aactggctga
     7561 gcataatctg ctccgccctt tcagccttag tctgttcgtc cacctgatta tcaaaggtcg
     7621 cggcaaatgt gccctcctcc gcggaatagg caaagcagcc cagacggtca aacttagcct
     7681 tttcaataaa gtcgcggagg gtcttaaact cctcctcgcc ctcgccgggg aaacccgtaa
     7741 taaatgtggt ccttataaca gcgtcgggca tatagcttct tatcttttct ataagagcca
     7801 gcaggctctc ggggtcgcct ctcctgttca tgcgccttaa aacttcctta tgggcatgct
     7861 gaaggggcag gtctatgtag tgaaggattt tctcattcga cgccatggtt tcaagcagct
     7921 catccgtaat cctgtcggga tagcagtaga gaatccttat ccatttaagc tcatctatct
     7981 tcacaagctc ccttaaaagc tcggggagca ttttcttgcc ataaatatcc tcgccgtagc
     8041 gggtggtgtc ctgagctatg agcataagct ccctgacgcc cttttcggca agctcctttg
     8101 cctccttaat aatgccctcc atgggtctgc tcctgaaggg acctctgatt tggggtatgg
     8161 agcagtaggt gcagttgttg gagcagccgt cggcgacttt cagataagcc cagaaaccgg
     8221 gggttgtgag aagcctttca ccctcaaggg gcatggcggt cttttcgggc tttacgctga
     8281 cggtctcgcc ctcaagaact cttttgcaca gctccacaat gtcgccgttg ccgccaagac
     8341 ctataacgcc gtccacctcg ggcatttctt tgagaatctc ctcctgatac ctttcggcaa
     8401 gacagccggt gacgatgatt tttcttacaa gtcccgactc tttaagctgc gccatctcaa
     8461 gaatgttctc gatagcctct ttcttagcgt cctcaataaa ggcgcaggtg ttaataatga
     8521 ttatgtccgc ataatctatc tcgtcaataa tttcaaagcc cgcgttattg agccttgcga
     8581 gcatcatctc gctgtccacc ctgtttttgg ggcagccgag agaaataaat ccaactctta
     8641 tacccatttt ctcaccttaa aaatattctg tgtccgtttg gaggctccca agcgcccttt
     8701 ctatatcata tagcttgtag ccccgtctta tgagattatt tataactctg cgcctttcct
     8761 tttcatcgga gagattcgcg gaaaagctgt cctcaataag cactatgagc cttttaaact
     8821 cgtcctcgtc gcttataaac tctacggcgg cgcgggctgt atcggaggaa actccccttg
     8881 atttcagctc atcaatgatt cgggctctgg caaagccctt acgctcaaaa agctcctcgc
     8941 caaaaaggga tgaaaactca gcgtcgttta taagccccat attctccagt tcatcggcgg
     9001 catattccgc cgcctcgccg tcgaactctc gtttgagctt ttgcacaagc tcgccccgtg
     9061 agtgcattct aagcgttata agccgcatag ccttgttcaa ggccgcggct tttgtctttt
     9121 cgtttattat gctgtctata tcttctacag ttatctcatc gccggctttg agctttaaat
     9181 ctcttaaaaa gccctctgtg acgatggcaa attgctctcc gtcggcaagc aggcgtacct
     9241 ttccgccttt aagtctttct atggaaaacc gcatcagtcg tccaccgtaa tatcaagaat
     9301 tgccctgttc gcggctacgc tctttttctc cgtttcgggc tgaacttccg cagtctcctg
     9361 aattacggag gagacatttc tcttagatac gctttcctct ttaagcttct ccctgatttt
     9421 cgcctcaatc tcggcggtca tttcggggtt ctgctcaaag aacagcttta cattgtccct
     9481 gccctgaccg agccttgtgt cgttgtatga gaaccacgcg ccgctctttt ggattatgcc
     9541 gtattttacg cccacatcaa ggatttctcc ctctctggag atacccttgc cgtacatgat
     9601 gtcaaactcc gcctctttga aggggggaga taccttgttc ttgacgattt tgaccttggt
     9661 gcgggagcct ataatctctc cgcccgttgc ggcggattta atctgctccg ctttcctgac
     9721 ctcaattctc atggaagcgt agaatttcag agcccttccg cccgttgtga cttcggggtt
     9781 gccgtagacc acgcccacct tttctctgag ctggtttata aagataacta tggtgttgga
     9841 gcggcttatc gcgcccgcga gttttcttaa tgcctgcgac ataagacggg catgcagacc
     9901 cacatgggag tcgcccatgc tgccctcaat ttcggagcgg ggcaccagag cggcgaccga
     9961 gtccacgacc accacatcaa ttgctcccga gcgggtcaga gcctctgtga tttccagagc
    10021 ctgctcgccg ttgtcgggct gggaaaccag aagggagtcc acatcaacgc ccagattcgc
    10081 ggcgtatacg gggtcaagag catgctccgc gtccacaaaa gcggcctcgc ctcccgcctt
    10141 ttgagcctcc gcaataacat gaagggcaag ggtggtcttg cccgaggatt cggggccgta
    10201 aatctctata attctgcctc tgggcagacc gcctatgccc gtagcggcgt caagggtcag
    10261 tgagcctgtg gggatagcct ctatgttcat ggcggtgctc tcgcccagcc tcataatggc
    10321 tcccttgccg aactgctttt ctatctgcgc cagagcggtt tccagggcct tctttctgtc
    10381 ggtagccttt tcgtttgcca tatatttatc tattcctttc gaaataagaa atttatcgca
    10441 caaatgttcg cggcttaata ggattataca gtttttcagg tgaaaaagca atacaaattt
    10501 ttgcggcaat agattcatag cctccataga aggaggctaa tgggtctaat cggtattttc
    10561 gggctttttg acatcgaacc agaactttac gccgccgctg acattctgca caccgtactc
    10621 catgcggtgg agcctttgaa tggcggcgac aagggcaagt cccagtccgc ttctgccggc
    10681 ggctcttgac atggccttgt ccgcgcggta gaaggaggtc cagattttgc ctatgtcctt
    10741 atctgcgatg tgcttgcccg tattgtagat gctgaccctc caaatctctc ccatgtcctc
    10801 tacgaaggct tttatatttt tgccctcgcc catatcctca acatgggaca tggcgttgga
    10861 aaggtagttg ttcacaacgg agccgagaat gattacatct ccgtagccca gagcgtcggt
    10921 cgggatggcg tttacaacgg ttacgccctt ttctttgagc tgcgccttgc ttcttacaaa
    10981 ccagtcttca accagctcaa agacattgaa tacctctcgg ttggggctgt attcgcccga
    11041 gtcgtactta ataatctcca ggagcttcat tatcatgtcg ttcatgcgtt ccgcttcgct
    11101 ccttataatg tccgcgtact cgagcgccat tttgggatcc tcttttataa agaccgacag
    11161 tccctcagcg tagccctgaa ttatggctat gggggtttta agctcatggg aaacgccggt
    11221 gatgaaaatc tgccttaagt ggtcaagggt gtgctctttt tctatgtcgt cctgaagttt
    11281 ttggttcttg gctttaaggt cggtcaaagc ttcgttgagc gaatccgaca gggcgttgat
    11341 actcttgccc agcctgtcaa tctcctcgcc ccgcatctcg cccactttct gagagaagtc
    11401 cagctttgac atattgtcgg taatgcggca catctcttta agcgggtcgg tgaacctctt
    11461 aataagcctt gccacgatga agagcattaa ggtgaccatt attgtcgaga gggtggagat
    11521 aaatataata cctaccctcg cgacatcgtc aatctcgttt ttgagcttaa acagctccag
    11581 ccgcccgtcg ctaaaatctc tgacaaagac aaggtattca cgctttacgg tgcccgatgt
    11641 gtcctctctt aaatcaaagt ggaagcctcg gttttgaatt cccgagtcca caatgacata
    11701 attcctttta tgctcgtcgc tgattttatc gtccttttta aagggaggct gtatgtaggg
    11761 acccgaatat gaggtggaat aaacaagatt gcctgtgggg ccgtagattt ctatgcttat
    11821 gtcgttgtta agctctacct ctcttaaagt attgtagaac ctgtcggggt cggagtaggc
    11881 gctttgaata atgtccgcgc tggtccacaa gtcaaagccg gtttttaaaa gatagacata
    11941 gttaatcatt ctggagtcgg caataacgac tatcgctatg cctatgatga aaaccgaggc
    12001 aatgcttctg aacagcagcg cgctgacgct aagcttcttt ttacttttct tctgtttcgg
    12061 agctttagtc ttctccgttc ttctcattag gctccacctc taacttgtag cccgcaccgt
    12121 aaattgtctt gatgtgcgct gctccccagt cgcccagctt ggtgcgcagg cgggccatgt
    12181 gagagtcaac ggttcttatg tcgccgtaga aatcatatcc ccatatgtcg tcaagaaggc
    12241 tctcccttga gaaaaccttg cccgcgttgg atacgagctt aaaaagaacg ctgtactctt
    12301 ttaatgtaag ggttacgctc tttccgttga gccatacctc gtgggcggtg tcgtcaatgg
    12361 taagcccgtc tatggagtaa accgactcgg aggtggcggc agccctttcg ttgcggcgaa
    12421 ttaaagcctc tattctcttt accagaacgc tggggctgaa gggctttgtg acatagtcgt
    12481 cggcgcccgc ctcaaaggcg gtcagctcgt caaattcctg acttcttgcc gtgagcataa
    12541 gaacgggcac attggagcgt tttctgatgc ggcggcagac ctcccagccg tcaacattgg
    12601 gcatcattat gtcaaggatg accgcgctaa aatcgctctc aaacttgtcg aacaggtcga
    12661 gcgcctcctg accgtcggca gcctcaacca tttcgtagcc gtcctttttg aaaaaatcca
    12721 cgaccagtcg tcttataagg tcctcgtcgt cggcaataag gatttttttc acttaaagca
    12781 gctccttccc ttttaaggtt aaacaacagc atttatttaa agttcttagc tttcaaaatg
    12841 caaagacttt ttcactcccg atttttcaat cgcgacatag agcacaagtc ccagtcccaa
    12901 gcaggctatg agctgacccg cagccaccga cagaactccg taggcaaagc cgataagacc
    12961 cgcgtaggcc ggcagcatga tataaagctc aagaccgatt accaaagcgt tcactataat
    13021 cggcggcaac gccgaaagaa caggcagacc cttgattttg taaggcttga agctccttgt
    13081 aaggattgcg gcaatcagcg tcgctactga gccgaagata atgtccacaa tgccgagggg
    13141 gctgccgaga ttgcctataa cgcagccgat aacaaggccg ggaattgccg cgggagtgaa
    13201 tacggggagt attgtgagca tttcggacag cctaatctga aaaggtccgt aagccaaatt
    13261 aaaggccgcc gaaatatagg tcaaagccac ataaagggcg gcgatgaccc ccgcctgagc
    13321 gatgtaggtg ataccgtttt tacttttcat ttccatcttc tttccgattc cgtagtttgt
    13381 tcgaagcctt aaaggcttcg gtcaggatgt tgcgactgac cgattttata aaggcgcccg
    13441 ccgcgccgtg aaatccccgc cgccgtgagc caaactccga cttttggggc ttgaccgaat
    13501 ttagccgagg agcgcgcccg ctttgcgggt ctcacccgcc gagcagccga tgggcgcaaa
    13561 atccctttat aaaaccgatt taattatatc agcaaataaa ataaccgtca acgaaacagt
    13621 tcaaatttaa catataagcg gcgcgaaaaa tataatttat aggcagattt aactatcttt
    13681 tcctcatttt agttttctaa agggtaatat tttattttac cgtccatctt ggagcggaga
    13741 ttgtccgctc gttgccggag ctcaacgcaa agaatactcc cgccgttcgc cccgccgtgg
    13801 agtcggggga cgcccgactg cgggcgggtg ttactcgccc cgccctgttg tgcggaagtt
    13861 gcccgcaaac ctgccgcaac tgaaccgcct ccgctggtgg ggtgtcgccg gttcatggga
    13921 gcggcacaaa aaaataggcg cggtcgcacg cctgcggggt ggggcgtctg caagggatac
    13981 ccgctcgccg tcgccgttgg gaaagggtcg ttcgcctttg cgggagcggt cgcccgttgc
    14041 ctccccgcac gccgagcggg acggggttgt ccgtctgcaa gtgagggcgc ctgtgtgaaa
    14101 agcaccccgc tcgccgttgt cgggagatgc ccgcctgtgt ggagggtgtc cgtccgctgt
    14161 cgggagcaca aaaaaaagct tgccgccgcc ttgttatgcg gcagtcaccc gcaaaacctg
    14221 ccgccccgaa ccgccgaccg tcggcgggat gcagtccgct ccccgcccgc cggcgggacg
    14281 gggtagccgc aaaaaatttc cctaccgctt gtcgcgctgg cggaagacgc ctgccctccc
    14341 cgaccgcggc gaaaaggcaa taaaaaaagg gagccttgcg ctccctatga ttttctttag
    14401 aaattaatat tgtcggggtc gccgcccagt ctgccgtagg cagagattga gcttattctg
    14461 cccatatcct cgtccgacag ctcaaagtcg aaaatcctcg cgttctcggc gatgtgctcg
    14521 ggattggtgg atctgggcag ggggagaata tccctttgca ggagccatct gagagttacc
    14581 tgagcgggcg ttctgccgta cttgtcggct acctctttaa gggtcgggtc agccataatc
    14641 ttgcccgaac ccaaggggct ccaagcctca acaacaacgc cgtggctgtg gcagtactga
    14701 accagctccg actgattaaa tccggggtga atctccacct gatttaccat aggtacaatc
    14761 tcggcgtcct ccaaaagcgc ctcgaaatga tgaataagga aattgctgac gccgattgcc
    14821 ttaatcctgc cctctttgta gagcttctcc atagcccgcc agctttcctt aatcttggtc
    14881 tgccagctgt ctctgaaatc cctcggaatg ggccagtgga taagataaag gtcaaggtac
    14941 tccagtccca gcctctccat ggtcgcgtca aaagctttaa gtgtcgtgtc gtatccctgg
    15001 tcggagttcc agagcttgct tgttataaaa atctcctctc tcggaatgcc gctttcttta
    15061 acagcggtgc cgacgcccac ttcgtttctg taaatggcgg cggtgtcaat atgcctgtag
    15121 cccgcttcca gggccttttt cgctgaattt attactgtct ctccctcggg aatctgccat
    15181 gtgccaaagc ccacgcaggg aattttaacg ccgttatgca ttacaaaaga atcggttaag
    15241 cttttcataa taaagcctcc tttttaaaag ccttattcac aattataaag acgcaaggta
    15301 taaaagtcaa ccgagcgggc agggaaatgg gaaaaaatag gatttaggtt gacaagcaaa
    15361 atattttgtt ttataatacc ctgtacaatt gcgtgagtta tacatttttt tggctttgcc
    15421 ccaaattttt aaacttgtgt gttttgtttg aatttatcgg catatgctaa ttgatgaaaa
    15481 gctcacagca gttaatcact atttttgaaa ggagaccttt ctttaatgga cgacccggac
    15541 cccggaggta cattcagtat tttgtcagcg gcagccctgc cggagctatt gcaagcggaa
    15601 cccgtaagtt ccgacggaat tcttcttaaa ctcgcgattc tcttcttcct cattcttata
    15661 aacgctttct tcgcgatgtc agagattgcg atagtatcct taaacgacgc gaagctggaa
    15721 aagctcgcat cgcagggcca caaaaaatca atgcagattc tggctcttac tcagaactct
    15781 gcggttttcc tctccaccat acaaatcggc gtaactctcg ccggcttcct cacaagctcc
    15841 tcggcagcac agtcattcgc tccgatgctt gtatctctga ttgaccccta tcttcccgac
    15901 gctgttccca ggggactgct tttgggagtg tcgacagtca tcatcacgct tatcacttct
    15961 tacttctcac tggttttggg cgagctcgtg cccaaaaaaa tcgcccttca aaaacccgag
    16021 caggtttcat atttcgcgat agggcttctt acattcg
//
LOCUS       DAONVQ010000028        16005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2713_length_16005_cov_4.934169, whole genome shotgun sequence.
ACCESSION   DAONVQ010000028 DAONVQ010000000
VERSION     DAONVQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16005
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2713_length_16005_cov_4.934169"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLD98_03070"
     CDS             complement(<1..483)
                     /locus_tag="PLD98_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQA05265.1"
                     /translation="MSKIIGIDLGTTNSCIAVIEGGEPIVIPNAEGARTTPSVVAFSK
                     TGERLIGQVAKRQAITNPDRTITSIKRQMGTDYTVKIDDKRFTPQEISAMILQKLKAD
                     AEAYLGTTITEAVITVPAYFTDSQRQATKDAGKIAGLDVKRIINEPTAAALAYGIDKE
                     N"
     gene            complement(524..1111)
                     /gene="grpE"
                     /locus_tag="PLD98_03075"
     CDS             complement(524..1111)
                     /gene="grpE"
                     /locus_tag="PLD98_03075"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQA05266.1"
                     /translation="MPKKKNPDINETSKEKDKEKDTPEKNAQAPENEKAEENKESAEL
                     QAVREELSNLKKEYDELKDIYLRTLAEYDNYRKRTLKEKDALYLDAKAEVLKNFLPVA
                     DNFQRALSASANEDLDSYKQGMGMIINQFLKALSDEGLTEFGNVGDSFDPNIHNCVMH
                     EENEELGENVISEVFQKGYMLGDRVVRAAVVKVAN"
     gene            complement(1140..2168)
                     /gene="hrcA"
                     /locus_tag="PLD98_03080"
     CDS             complement(1140..2168)
                     /gene="hrcA"
                     /locus_tag="PLD98_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HQA05267.1"
                     /translation="MELSVRKNKILAAVVDRYILTGEPVGSKTVAAMLDHAVSSATIR
                     NEMAELCELGYLEQPHTSSGRIPTQKGYRYYVDRLMGRYELASDEMYRIERQLESASS
                     EPEALLIKASAILAELTGCAGISTTPKDEYTVIKKVELVPIGKKTIMVVLATSSGLLK
                     SKVCRMDVPITLDLIELFYNVAAANFIGVRVSDIHAPLLQNIAASLGEKAFDMLGLVN
                     AVADLAQICTSSELVLEGEANLLNHKELEANALELLTFLQDGDLLSKMLAERNSQLSV
                     TIGKENVFKELENSSMILSSYSVLGQRAGSVGIIGPTRIDYARLIPSIKFLSQLVGKL
                     LSDVTESD"
     gene            complement(2418..2879)
                     /locus_tag="PLD98_03085"
     CDS             complement(2418..2879)
                     /locus_tag="PLD98_03085"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HQA05268.1"
                     /translation="MKRIASFEVNHNILERGMYTSRIDGDAVTYDIRMRRPNKEPVIS
                     NAAIHTIEHLFATFVRNSEYAQGIIYFGPMGCRTGFYFIVRDSISPDTAIKLAHDAFK
                     FIAEYEGEIPGATEIECGNFREHSLEEAKKEAADFYPVLENWTAEMLKYKE"
     gene            complement(2892..4931)
                     /gene="recG"
                     /locus_tag="PLD98_03090"
     CDS             complement(2892..4931)
                     /gene="recG"
                     /locus_tag="PLD98_03090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQA05269.1"
                     /translation="MNLETSIQYVKGVGEARAKQFKKLGVETVGDLLFFFPRAYENWT
                     PESSIHKAPLYTNCCIRAYIAEPPIVSRISGGRLLTKCSATDGETIMNLVFFNNPYIA
                     DKLIEGKEYLFYGKLSRNLYGGREMTSPRLGSVELAGKVRPVYHQTAALKSAVIENCV
                     KNCFSSLKDLPETLPEYLISKYDLMPRYEALRQMHFPDNGDMLFKARRRLVFEELLIF
                     RLALFLIREKGSENDGIALKKDYTDEYAQYLPFTLTNAQKKCIREIAADLSGTKPMNR
                     LLQGDVGSGKTAVAAAAIYSCAKNGYQSALMAPTEVLAEQHYKTLLRLFEGTDLNICL
                     LTGSKTAKEKRKAKESLALGETDIAVGTHALLQSDVEFKKLGLVITDEQHRFGVNQRA
                     ALAAKGDNPHVLVMSATPIPRTLALIVYGDLDVSVIDELPPGRQVIETYKVNSGYRQR
                     IYRFIKKHLDEGRQAYIICPLIDSDEEEPADLIAAKELFEELSKGEFKNYKVGLLHGK
                     MRPNEKNNTMRLFSEGEIQLLVSTVVVEVGVDVPNATVMVIENAEMFGLSQLHQLRGR
                     IGRGKHKSTCILVSDDEGENAKQRFEVFCSTTDGFVIAEKDLELRGPGDFFGERQHGI
                     PQFKIADIFTDSKSLYAAQKIAEGILSLDPRLQRAENRMLKESVDRLIADFQKAN"
     gene            complement(4959..6602)
                     /locus_tag="PLD98_03095"
     CDS             complement(4959..6602)
                     /locus_tag="PLD98_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HQA05270.1"
                     /translation="MITGAMLRDAVISAANNIGNYRTQVDELNVFPVPDGDTGTNMSM
                     TIQNAAKAVRFVEDSASIEQVAKIVSSALLRGARGNSGVILSLIFRGFEKGLRGCFEA
                     AGPTIATALKLGAEQAYKAVMKPVEGTILTVIRVAAEEAEKAADEESAEKVWEIACEA
                     AKRALADTPNLLPALKKAKVVDSGGQGLVYIMEGMRSVFSDNVIIEGESVEPQAAAVI
                     RDVIHSEEQIKYGYCTELLISKKPGSFLDSLLLRAYLETIGDSVVVVDDDDIIKVHVH
                     TDDPGSVLTKAKEYGEFENIKIDNMRRQHRNIVWGANQHMDTSGTHDESAEMKDFGFV
                     AVAAGEGLMELFTSLGVDRMVSGGQTMNPSTEDILNEINKTPANTVFVLPNNKNIILT
                     AEQTLSLTSKKVRVIPTKTIPEGIAALLAFDPDADEETNHLNMNKAAEQVQTAQVTFA
                     ARDSVVNGQNVKKGQMLGMENGKITVIDDNPVSAAFKTTKRLLKKSTSVITIIYGEGV
                     TEEDAEKLKKLIEAKAPSGAEVVVVNGGQPVYYFIVSVE"
     gene            complement(6621..6974)
                     /locus_tag="PLD98_03100"
     CDS             complement(6621..6974)
                     /locus_tag="PLD98_03100"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HQA05271.1"
                     /translation="MVRIENHLGTIDISEKYFSSLIGHTVVSCFGVLGIADGSTVKGK
                     NKSRSFKKKGSGKGVTVKSELGGLLVDIHIIVGYGINISAIVNSIKTKVLYVVETATQ
                     LKVKRINVFVDAMIS"
     gene            complement(7092..9485)
                     /gene="pglX"
                     /locus_tag="PLD98_03105"
     CDS             complement(7092..9485)
                     /gene="pglX"
                     /locus_tag="PLD98_03105"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HQA05272.1"
                     /translation="MTYPEVLNSEGRRLLKVFALSSRNALLKILNGSPASLTQGEAAL
                     NSKEYADAENTAYSVFILSVFSKYLISNGLAELEDTGESILTLIKGYESLVPLALRGC
                     FSYFISEANRFDADIFNNKVFGEFLKILNDLDSRYWIENPEIIGILHQYFHAEAKDRA
                     INAYKGRIEKEQIYAATQLFTPAWVVKYLAENSLGKLWAKIKPSSPALKGFKYFVPSA
                     DEENENTVFTNEEIIEKLKSVSFLDPCAGSGNILMYTLELFVMLYTECGLSTLSACEN
                     ALKHNLFGMDIDERMRAVAAFSLLAKAAVFDKEILSLPVSVNFVILKGSGGLELSSSL
                     IEALGEDLAAKAQKLIDEFALCDEAGCLINPSGCADFADELSGVLISDKLSEPDKMTL
                     NRLIALSKQAAALKRKYSVVVTNPPYSNKPSKRLKAYLLKEYKAYAGDLFSAVMKRCF
                     EFCDKDGYCAFLTPYVWLFIANHKALRKEIINGKSLESLLLFEYSAFADATVPLCAFA
                     IKNQNENKKSIFIGLDEFRGGMDVQEKYMLSSLKEADCPYKYIRDTKTFLDLPNYSFA
                     FWAEPPLIEAFKNHKRIVDIAAPRQGLATGDNSRYVRFWHEVSFEDIGQGFGSLNEFL
                     KSGKKYMPYNKGGSFVRWYGNLDRVLRIDRPYFEELQNRGNRLPSRQLYGKECISWSK
                     VTSSDFSMRYIPKGFAFDVAGCSVFADSELWCLLAFSNSSTMQALLNVLSKTLNYEVG
                     AVKSIPYAELPAERKAEAERLAKESVAALKDEWDRYEQSRDFKCNPLVKIAKEKRNS"
     gene            complement(9472..11244)
                     /gene="aspS"
                     /locus_tag="PLD98_03110"
     CDS             complement(9472..11244)
                     /gene="aspS"
                     /locus_tag="PLD98_03110"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQA05273.1"
                     /translation="MELLKGLKRTNYCGEIRENHIGSEVTVFGWVQRVRNLGGLIFID
                     LRDRTGIVQLSFGDETDREVFEKAASVKSEYVLAAKGFVAERASKNPDLPTGAVEIKV
                     NELRILAKSETPPFEIVENSNTSEAIRMKYRYLDLRRPDLQRNILLRSKAAKVTRDYF
                     YENGFVEIETPILIRSTPEGARDFLVPSRVHKGSFYALPQSPQLYKQLSMVAGFDRYI
                     QLARAFRDEDLRADRQPEFTQIDLEMSFVDVEDVLQIVEGYIARVFKEVVDIDVPLPL
                     PRLTYEEAMNRYGSDKPDTRFGLELIDFSDAVKGCGFKVFDSAIESGGIVRGFNAKSA
                     AAVLTRKEIDKLTETAKGIGAKGLAWIRLTDEGVSSSFGKFVSEDVMNEILKKAEAEK
                     GDVILIVADKKSTALSVLGHLRNETARKLDIIPDRYNFLWITEFPFFEYDEESGQYVA
                     MHHPFTSPMDECLEYLETDKSKVKAKAYDLVLNGIELSSGSIRITDPELQSRIFSLLG
                     LSKEEAQAKFGYLLDAFRYGAPPHGGMGIGFDRLLMQMLKAESLRDVVAYPKLKDGTE
                     PMTDCPAVVDENQLKELGLKIDLS"
     gene            complement(11268..12629)
                     /gene="hisS"
                     /locus_tag="PLD98_03115"
     CDS             complement(11268..12629)
                     /gene="hisS"
                     /locus_tag="PLD98_03115"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HQA05274.1"
                     /translation="MLTKAPRGTHDILPSEIHKYQFLEDEFLSLAESFGFKEIRIPVF
                     EHTELFHRSVGETTDVVQKEMYTFNDLSGRSITLRPEGTAGVARAYVEHGLFNEPAPQ
                     KFVYKVSCYRYEKPQAGRLREFHQFGVETFGASDSSAEAEIVALADAFFELLGIEDIH
                     LEINSIGCPECRKVYLEKLRAYFKEHIDSLCDTCKDRLERNPMRILDCKSPVCKEISK
                     GVPIMLDSLCGDCKEHFESFKADLDALGVEYKVNPMIVRGLDYYTRSVFEFVSDKIGT
                     QGTVCGGGRYDGLIEEIGGPATPACGFGMGVERLVLLLESIGFDFPEPSPCALYIAST
                     DKEAARVASQIAFDVRLSGLGAEYDITGRSLKAQMKYANKINADFTLVLGNDEIMSGV
                     GRLKDMKTGESVEVRLDEVAEAIVDIYSNRIVEQFGELSGSFPDDGGDSPTFTIIEGG
                     KKK"
     gene            complement(12868..13275)
                     /gene="mgsA"
                     /locus_tag="PLD98_03120"
     CDS             complement(12868..13275)
                     /gene="mgsA"
                     /locus_tag="PLD98_03120"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HQA05275.1"
                     /translation="MNIALIAHDAKKELMIQFCIAYCGILSRHSLCATGTTGKLVAEA
                     TGLEIQRFLSGSQGGDQQIAARIACNEIDLLLMFRDPLTAKPGEPNEMNLLHLCDVHN
                     IPVATNIATAEVLVRGLERGDLDWRDLVNPKNK"
     gene            complement(13548..14282)
                     /locus_tag="PLD98_03125"
     CDS             complement(13548..14282)
                     /locus_tag="PLD98_03125"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HQA05276.1"
                     /translation="MASGKGGTGKSTMTAAIGRALARQGKRVLLADMDAGVRALELML
                     SADSLTHYNWLDAATGKARVQDAVSSLGQNLSLLHAPNEGGVQVEPSEIKEMLSALSA
                     NFDYILCDSSAGVGQSLKIAAAASDAAIIVTVPDKIAVSAAAAASEFLFSEGVEDISL
                     IVNKFSVNRLPCSKEDFFTLDDIMDTVCARLLGVVPFDSSVVSLVKGENASGKSAVEA
                     SVQRIAKRIEGESVPLPVKELIKYKG"
     gene            complement(14335..15510)
                     /locus_tag="PLD98_03130"
     CDS             complement(14335..15510)
                     /locus_tag="PLD98_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQA05277.1"
                     /translation="MPTYTEDFTTIPFGPLGIIATPGCEELAAKIDKYIVEWRANRES
                     EHKTTIAFAGYQRDSYIIDVKFPRFGSGEAKALITESVRGYDLFILTDVFNYGVTYKM
                     YDMVVPMSPDDHYANLKRAIAAVAGKAHRITVIMPMLYEGRQHKRSSRESLDCAIALQ
                     ELCHTMGVDNIITFDAHDARVQNAIPLKSFENVTTVYQMIKALVNTVDNLKIDKDHLM
                     IISPDEGGMSRCIYYSTVLGIDLGMFYKRRDYTRIVDGRNPIVAHEYLGANVEGKDVV
                     IVDDMISSGESVLEVASKLKSMKAKRIFICAAFGLFCDGLANFHKAYKDGIIDRVFTT
                     NLVYRSPELLASEWFTEVDMSKYVSLLIDTLNHDLSISKLLSPNERIHRFLKLKGYK"
     gene            complement(15784..>16005)
                     /locus_tag="PLD98_03135"
     CDS             complement(15784..>16005)
                     /locus_tag="PLD98_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQA05278.1"
                     /translation="GVKSVCGLGLMVGIETEAPPADVAQKAVENGVLVLTAKTKVRLL
                     PPLNIPFEDLKRAVEILKKVIAEEAEKVK"
BASE COUNT         3905 a         4038 c         3610 g         4452 t
ORIGIN      
        1 gttttcctta tctatgccgt aagccaatgc ggcggctgtg ggctcgttga tgattctctt
       61 aacatcaaga cccgcaattt tgccggcgtc ctttgtagcc tgacgctggg agtcggtaaa
      121 gtatgcggga acggtgataa ccgcctcagt gattgtggtg ccgagataag cctccgcgtc
      181 tgccttcagc ttctgcagaa tcattgcgga aatctcctga ggggtgaacc tcttgtcatc
      241 tatcttaacg gtgtagtccg tacccatctg gcgcttgatt gatgtaatgg tcctgtcggg
      301 gttggtgatt gcctgccttt ttgcaacctg acctataagc ctttcgcctg tttttgagaa
      361 cgctacaacg gagggtgtgg ttcttgcgcc ttccgcattg ggaataacta tcggctcacc
      421 gccttctata accgctatgc aagagttggt tgtgccaagg tcaatgccta ttatttttga
      481 catgtttgtt cctcctgaaa tgatataggt ttataaaata gagttaattg gctaccttaa
      541 caaccgcggc tctgacgacc ctgtcgccga gcatatagcc cttttggaaa acctcggaga
      601 tgacattttc gccaagctcc tcgttctcct catgcattac gcagttgtga atattggggt
      661 caaagctgtc tccgacattt ccgaactcgg taagcccctc atcggagaga gcctttaaga
      721 actgatttat tatcattccc atgccttgct tatagctgtc aaggtcctca ttcgcgctcg
      781 cagataaagc cctttggaaa ttgtccgcga cgggcaggaa gttctttaaa acctccgcct
      841 tagcgtccaa ataaagggcg tctttttctt ttaatgtgcg ttttctgtaa ttgtcgtact
      901 ccgcaagggt gcgaagatat atatccttta attcgtcgta ttccttttta aggttcgaaa
      961 gctcttcccg aacagcctga agttcagcgg attccttatt ctcctccgcc ttttcgttct
     1021 caggcgcttg agcgttcttc tcaggtgtgt ccttttcctt atccttttcc tttgaagtct
     1081 cgtttatgtc gggatttttc tttttaggca ctaatatcaa tcctcactgt atatttttgt
     1141 caatcagact ctgtgacatc gctcagtagc ttgcccacaa gctgggacaa gaacttaatg
     1201 ctgggtatca gccttgcgta gtctattctg gtaggtccga taatgcccac agagcctgcc
     1261 ctttgaccaa gaaccgaata gctggagagt atcatgctgg agttttcaag ctccttaaaa
     1321 acattctcct tgccgatggt gacgctgagc tgagaatttc tctccgccag cattttggag
     1381 agcaaatctc cgtcctgaag gaaggtcaaa agctccaagg cgttcgcctc aagctccttg
     1441 tggtttaaaa ggtttgcttc gccctcaagc acaagctccg agcttgtgca gatctgagcc
     1501 aagtccgcta ccgcgttaac aagacccagc atatcgaatg ccttttcacc gagcgacgcg
     1561 gcgatattct ggagcagggg agcgtgaatg tccgaaaccc tgacacctat gaaattcgcg
     1621 gccgccacat tgtaaaaaag ctcaataagg tccaaagtaa tgggcacatc cattcggcag
     1681 accttagact ttaaaagtcc cgatgaggtt gccagaacca ccattatggt ctttttgccg
     1741 atgggaacaa gctcaacctt tttaataacc gtgtattcgt ccttgggcgt ggtggaaatg
     1801 cccgcgcagc ccgtcagctc cgcaagtatc gcgcttgcct ttataagcag agcctcaggc
     1861 tcgcttgaag cgctttcaag ctgacgctct attctataca tctcgtcgct tgcaagctca
     1921 tatctgccca tcaggcggtc tacataatac ctgtatccct tctgagtcgg gattcttccc
     1981 gatgatgtgt ggggttgctc aaggtaacca agttcgcaaa gctcagccat ttcgttcctt
     2041 attgtagcgg aagaaaccgc atggtcaagc attgcggcaa cggtctttga gcccacaggt
     2101 tcgcctgtaa gtatataacg gtcaacaaca gctgcaagta ttttattctt ccttacgcta
     2161 agctccacaa ccccacctcc ttctgtttaa tagcactgtt agcactctta tatgctgagt
     2221 gctaacaata ataatatata gtcctaatca gcttttgtca accgaaattt tgtaaataag
     2281 ttgttaattt agaaacgctt gcttcttttc cggcaaggaa agctgcggaa ttaaactaaa
     2341 gccgcacata ttattttccc ctcagagatt aatatattcg aaacaaaaag ccttccgctt
     2401 ttgcgaaagg ctttagctta ttccttgtac tttagcattt ccgccgtcca gttttcgaga
     2461 acggggtaga agtcagcggc ctctttttta gcctcctcaa gggagtgctc tctaaagttg
     2521 ccgcattcaa tctcggttgc tccggggatt tcgccctcgt actccgcaat gaatttaaaa
     2581 gcatcatggg caagctttat agcagtatcg ggggaaatgc tgtccctgac tataaagtaa
     2641 aaacccgttc tgcagcccat gggaccaaag tagataatgc cctgggcata ttccgaattc
     2701 cttacgaatg ttgcaaacaa atgctcaatt gtgtgaatgg cggcgttgct gattacaggc
     2761 tctttattgg gccttctcat tctaatgtca tatgtcacag cgtcgccgtc gattcttgag
     2821 gtatacatgc ccctttcgag tatattgtga tttacctcaa agcttgcgat tcttttcata
     2881 ttcttcaccc tttaattagc tttctggaaa tcggcgataa gtctgtcaac cgactctttt
     2941 aacatcctgt tctccgccct ttgaaggcgc gggtcaaggg acaaaatacc ttcggctatt
     3001 ttctgagccg cgtaaaggct cttgctgtcc gtaaatatat ccgcaatctt aaactggggt
     3061 atgccgtgct gcctttcgcc gaaaaagtcg ccgggtcccc tcagctcaag gtccttttca
     3121 gcaataacaa agccgtctgt ggtggagcag aaaacttcaa agcgctgctt tgcgttttct
     3181 ccctcatcgt cggagacaag tatgcaggtg gatttgtgct tgcccctgcc gattctgccc
     3241 cttagctgat ggagctgaga cagaccgaac atctcagcgt tttctatgac cattacagtg
     3301 gcgtttggca catccacgcc cacctcaacc acaacggtgg aaacaaggag ctgaatttcc
     3361 ccttcggaaa acagccgcat tgtattgttc ttttcgttcg gtctcatttt gccgtggagg
     3421 agacccacct tatagttttt gaattctccc ttggacagct cctcaaaaag ctcctttgcg
     3481 gctatcaggt ccgcaggctc ctcctcgtcg ctgtcgataa gcgggcaaat gatataagcc
     3541 tgcctgccct cgtcaaggtg cttttttata aatctgtata tcctctgacg gtagccggag
     3601 tttaccttat aggtttctat tacctgcctg ccggggggca gctcatcgat gacactcaca
     3661 tccaaatcgc cgtagactat gagggccaga gtgcggggga tgggcgtcgc cgacataaca
     3721 agcacatggg ggttgtcccc cttagcggca agagccgccc gctgattaac tccgaacctg
     3781 tgctgttcgt ctgtgattac aagccccagc ttcttaaact ccacatccga ctggagcagc
     3841 gcatgggtgc caaccgcaat atcggtttcg cccaaagcaa ggctctcctt agcctttctc
     3901 ttttccttcg cggtctttga gcctgtcaga aggcagatat tcaaatccgt gccctcaaac
     3961 agccttaaaa gtgtcttgta gtgctgctcg gcgagtacct ccgtcggagc cataagagcg
     4021 ctttgatagc cgttttttgc gcaggaataa atcgccgccg cggcgaccgc tgtcttgccc
     4081 gagccaacat cgccttggag tagacggttc atgggctttg tgcccgaaag gtcagccgca
     4141 atttctctta tgcacttttt ctgcgcgttt gtaagcgtaa agggcaggta ctgggcgtac
     4201 tcatcggtat agtctttctt tagagctatg ccatcatttt cgcttccctt ttcccttata
     4261 aggaacaaag cgagcctgaa aatcaaaagc tcctcaaaaa caagccttcg ccttgcttta
     4321 aacagcatgt ccccattgtc gggaaagtgc atctgcctta aagcctcata gcggggcata
     4381 aggtcatatt ttgatattag gtattcgggc agggtttcgg gcaaatcttt aaggctcgag
     4441 aaacagtttt ttacgcagtt ttcaataacg gcggacttta atgcggcggt ctgatggtat
     4501 acgggtctaa cctttccggc aagctccacg gagccaaggc ggggagaggt catttccctt
     4561 ccgccgtaga gatttcttga gagcttgccg taaaaaagat attccttgcc ttcaatcagc
     4621 ttgtccgcta tataggggtt attaaagaac acaaggttca ttattgtttc cccgtcggtg
     4681 gcgctgcatt ttgtaagcag cctgccgccg cttatcctgc tgacaattgg aggctcggct
     4741 atatacgctc ttatgcagca gttcgtatat agcggagcct tatgtattga gctttcgggt
     4801 gtccagtttt cataggcgcg gggaaagaag aaaagcaggt cgcccaccgt ttcgacgccc
     4861 agctttttaa attgcttggc ccttgcctcg ccgacacctt tcacatattg aattgaagtc
     4921 tcaagattca cattccgtcc ctgcctttaa aaggggcgtt attcaaccga aactatgaaa
     4981 tagtaaacag gctgcccgcc gtttaccaca acgacctcgg cgcccgacgg agccttagct
     5041 tcaatcagct ttttaagctt ttcggcgtcc tcctcggtta cgccctcgcc gtaaattatc
     5101 gtaatgacgg aggtggattt ctttaaaagc cgctttgtgg tcttgaacgc cgcgctgacg
     5161 ggattgtcgt caataacggt aatcttgccg ttttccatgc ccagcatttg accttttttg
     5221 acattctgac cgttcacgac gctgtccctt gccgcaaagg tgacttgcgc ggtttgaacc
     5281 tgctcggctg ccttgttcat gttgaggtga tttgtctcct cgtccgcgtc cgggtcaaag
     5341 gcaagcagag ccgcaatacc ttcgggtatg gtctttgtgg gaatgaccct tacctttttg
     5401 gaggtcagtg acagggtctg ctccgcggta aggattatat ttttattgtt cggcagaaca
     5461 aaaaccgtat ttgcgggagt cttgttaatc tcgttcagga tatcctctgt gctgggattc
     5521 atggtctgtc cgccgctgac cattctgtca acgccgagag aagtgaacag ctccatgaga
     5581 ccctcgccgg cggcaacggc gacaaagccg aaatccttca tctccgcgct ttcgtcgtgg
     5641 gttcccgagg tatccatatg ctgatttgcg ccccaaacta tatttctgtg ctggcggcgc
     5701 atattgtcta tcttgatgtt ttcaaattcg ccgtactcct tagcttttgt cagtacgctg
     5761 cccgggtcgt cggtgtggac atgcaccttt atgatgtcat cgtcatcgac caccacgacg
     5821 ctgtcgccaa tggtttcaag gtaagctctt aaaagcaaag aatcaagaaa gctgccgggt
     5881 tttttggaga taagcagctc tgtgcaatag ccgtatttta tctgctcctc ggagtgaata
     5941 acatctctga taactgcggc agcctggggc tccacgcttt ctccctcgat aataacattg
     6001 tcggagaaaa cgctgcgcat gccctccatg atatatacaa gaccctgacc gcccgagtcc
     6061 acaacctttg ccttctttaa agcgggcagg agattgggag tgtcggcaag agcccttttc
     6121 gcggcctcgc aggcaatttc ccaaaccttt tccgcggact cctcatccgc agccttttca
     6181 gcctcctcgg cggcaactct tatgacggta aggattgtac cctcaacagg cttcataaca
     6241 gccttgtaag cctgctccgc gccaagtttt aaagcggtcg cgatggtggg accagccgcc
     6301 tcaaagcagc ctctcagtcc cttttcaaag cctctgaaaa taagggaaag gataacgccc
     6361 gagttgcccc ttgcccctct taaaagggca gaggaaacaa ttttggcgac ctgctcaata
     6421 gaggcgctgt cctcaacgaa tctaaccgct ttggcagcgt tctgaatggt catggacata
     6481 tttgtacccg tatcaccgtc gggaacggga aaaacattca gttcatccac ctgtgttcgg
     6541 taattgccga tattgtttgc cgcggaaatt acggcatctc taagcattgc acctgtaatc
     6601 aaaaataaca ctccaagcaa tcaggaaatc attgcgtcaa caaaaacatt aattctttta
     6661 actttaagct gggttgctgt ttccaccaca tagagaacct tagtttttat gctgtttaca
     6721 attgcggaga tattaattcc gtagcccacg atgatgtgaa tatccacaag aagaccgccc
     6781 agttcgctct tgactgtgac gcccttgccg gagccctttt tcttaaaaga gcggctctta
     6841 tttttgccct ttactgtgct tccgtcggct attcccaata ctccgaagca tgacacaaca
     6901 gtatggccga taagacttga aaaatatttt tcggaaatat ctatggttcc aagatggttc
     6961 tcaattctta ccatttacag ccctccagca ataaactcac agccattata actgttatgc
     7021 gccgatttat caacattatt aaagctaatt tgcccctgcg gacatgggaa taagcaaatt
     7081 ttagaaaaat cttaagaatt tcttttttcc ttcgcaatct tgacaagggg gttgcattta
     7141 aaatcccttg actgctcata tctgtcccat tcatccttca aagcggcgac agattctttc
     7201 gccagccttt cagcttctgc ctttctttcg gcggggagct cggcgtacgg aatggatttc
     7261 accgcgccga cctcatagtt taaggttttg cttaacacat tcaaaagagc ctgcatggtg
     7321 ctggagttcg agaaagccag aaggcaccaa agctcactgt cggcaaagac gctgcagccc
     7381 gccacatcaa atgcaaagcc tttcggaata tacctcatcg aaaaatcgct cgaagtcacc
     7441 tttgaccagc taatgcactc tttgccgtag agctgccttg aaggcaggcg attgcccctg
     7501 ttctgcagct cctcaaaata tgggcggtca attcttaaaa ccctgtcaag atttccgtac
     7561 cagcggacga aactgccgcc cttgttgtag ggcatatact ttttgcccga ttttaaaaac
     7621 tcgttaagac ttccaaagcc ctgacctata tcctcaaagc taacctcatg ccaaaagcgg
     7681 acatagcggg agttgtcgcc cgtcgcgagt ccctgacggg gagcggcgat gtccacaatc
     7741 ctcttgtgat tcttaaacgc ttcaatcagc ggaggctccg cccaaaaggc gaaagagtaa
     7801 ttgggcaagt caaggaatgt ctttgtatcc cttatatatt tatatggaca gtccgcttct
     7861 ttcagagatg aaagcatata tttttcctga acatccattc caccgcggaa ctcgtcgagg
     7921 cctatgaata tggacttttt gttttcgttt tgattcttta tcgcaaaagc gcaaagggga
     7981 actgttgcgt ccgcgaatgc ggaatattcg aacaaaagca gagattcaag gcttttgccg
     8041 tttattattt cctttctgag agccttatga tttgctataa acagccaaac ataaggagtt
     8101 aaaaaggcac agtagccgtc tttgtcgcaa aactcaaagc accgtttcat tactgcggag
     8161 aaaaggtcgc ccgcataagc cttgtactct ttaagaagat acgccttcag tctttttgac
     8221 ggcttgtttg aatagggcgg gttagtgacc acaaccgagt atttgcgttt taaagccgca
     8281 gcctgctttg agagcgcaat caatcgattt aatgtcattt tatctggttc ggaaagttta
     8341 tcggagatta aaacaccgct taattcatcg gcaaaatcgg cacagccgga aggattaata
     8401 agacagcccg cctcatcgca gagggcgaat tcgtctatca gcttttgagc tttagccgcc
     8461 aaatcctcac cgagagcctc aattaaagaa ctcgacagct caagaccgcc gctgcctttt
     8521 aaaataacaa agtttacact gacaggcagt gacaaaatct ccttgtcaaa gactgcggcc
     8581 ttagcaagca aagaaaatgc ggctacagcc ctcatcctct cgtcaatatc catgccgaaa
     8641 agattgtgtt taagagcatt ttcgcaagcg cttagggttg ataaaccgca ctcagtgtag
     8701 agcattacaa aaagctccag agtgtacatc aggatattcc cgctgccggc gcatgggtca
     8761 agaaagctga cactctttag cttttctatg atttcctcat ttgtgaaaac agtattttcg
     8821 ttttcttcat cggcagacgg tacaaaatac ttaaaacctt taagggcagg ggaggagggt
     8881 tttattttcg cccacagctt gccaagtgag ttttccgcca agtactttac aacccatgcg
     8941 ggggtgaaaa gctgagtagc tgcgtagatt tgctcctttt cgattctgcc cttgtaggcg
     9001 tttatcgccc tgtctttagc ttccgcgtga aaatactgat gcagtatgcc tataatttcg
     9061 gggttttcta tccagtacct gctgtccaag tcattaagaa tcttcaaaaa ctccccgaaa
     9121 accttattgt taaaaatatc ggcatcaaat ctgtttgcct cggaaataaa ataagaaaaa
     9181 cagcccctaa gagccaaagg cacaaggctt tcgtatcctt taataagtgt cagaatactc
     9241 tcgcccgtat cctccaactc agcaaggccg ttcgatatta ggtattttga aaaaacagac
     9301 aggatgaaca cagagtaggc ggtgttttct gcgtccgcat actccttaga atttaaagct
     9361 gcctctcctt gtgtcagaga ggcggggctt ccgtttagta tctttaacag cgcgtttctg
     9421 gagctgaggg caaagacctt taaaagcctt ctgccctcag agtttaaaac ttcaggataa
     9481 gtcaatttta agacccagct ccttaagctg attttcgtca acaactgcgg ggcagtcggt
     9541 catgggctct gtgccgtcct tgagcttcgg gtacgccaca acatctctca ggctctcggc
     9601 tttaagcatc tgcataagca gcctgtcaaa gcctatgccc atgccgccgt ggggcggtgc
     9661 gccgtacctg aacgcgtcaa gcagatagcc gaactttgcc tgagcctcct ctttggagag
     9721 accgaggaga gagaaaatgc ggctttgcag ctcgggatcg gtgattctga ttgagccgct
     9781 tgaaagctca atgccgttga gcaccaggtc ataagccttt gccttaacct ttgatttgtc
     9841 ggtctcaagg tattcaaggc actcgtccat gggcgaggta aagggatggt gcatagccac
     9901 atactgaccg ctttcctcat cgtactcaaa gaaggggaac tcggtaatcc acaggaagtt
     9961 ataccggtct ggtataatat caagcttcct tgcggtctcg tttctgagat gtcccagaac
    10021 cgaaagggcg gtggatttct tgtctgcgac tataaggatt acatcgccct tttcagcttc
    10081 agctttctta agaatctcgt tcataacatc ctcggaaacg aattttccga aggatgatga
    10141 aacgccctcg tctgtaagtc ttatccacgc aagaccctta gcgccgatgc cctttgcggt
    10201 ctcggtgagt ttatcgattt ccttgcgggt cagcaccgcc gcggcagatt ttgcgttgaa
    10261 gcctcttaca atgccgccgc tctcgatcgc ggaatcgaat accttaaatc cgcagccctt
    10321 tacggcgtcg gagaagtcga taagctccag accgaacctt gtgtcgggct tatcggagcc
    10381 gtagcggttc attgcctctt cataagtgag ccttggcagc ggcagcggaa catcgatgtc
    10441 cacaacctct ttaaagactc ttgctatata gccctcaaca atctgaagga catcctccac
    10501 atccacaaag gacatttcaa ggtcaatctg agtaaactcg ggctgtctgt cagctcttag
    10561 gtcttcatct ctgaatgccc ttgcaagctg aatatacctg tcaaagccgg ccaccatgga
    10621 aagctgcttg taaagctggg gcgactgggg cagagcgtaa aagctgccct tgtgcactcg
    10681 gctggggaca aggaagtccc ttgctccctc gggcgtggag cggataagaa taggtgtctc
    10741 tatctcaaca aagccgttct cgtagaaata atcccttgta acttttgccg ccttgctgcg
    10801 aagtaagata ttcctctgca aatcgggacg gcgcaggtca agatagcgat acttcatgcg
    10861 gatagcttcc gaagtattcg agttctccac aatctcaaag ggaggagtct cgctctttgc
    10921 gaggattcgc agctcattaa ccttaatttc aacggctccc gtgggaaggt cgggattctt
    10981 tgatgccctc tcagccacaa agccctttgc ggcaaggaca tattcgctct ttacggaggc
    11041 ggccttctcg aaaacctccc tgtcggtttc atcgccgaat gagagctgaa caatgcctgt
    11101 tctgtctctt aaatctatga aaataagccc gccgagattc ctgactcttt ggacccagcc
    11161 gaaaactgtg acttctgagc ctatatggtt ttctctgatt tctccgcagt aattggtgcg
    11221 ctttaatcct ttcagcagtt ccatcgtttg accatgcctt tcgaaagtta tttctttttt
    11281 ccgccttcga ttatggtaaa tgtggggctg tcgccgccgt catcggggaa agagcccgac
    11341 agctctccga attgctcaac tatcctgttc gaatatatat ccacgatagc ctctgcaact
    11401 tcgtcgagcc tgacttcaac gctttcgccc gttttcatgt ctttgagcct gccgacgccg
    11461 ctcattatct catcgttgcc gagtaccagc gtaaagtcgg cgtttatctt gttagcgtat
    11521 ttcatctgag ctttaagcga gcggcctgta atgtcgtact ccgcgcccag accgctgagc
    11581 cttacatcaa aagcaatttg cgacgcgacc cttgcagcct ccttgtcggt agaggcgata
    11641 tacaatgcgc agggcgaggg ctcggggaag tcaaagccta tggactcgag aagcaagaca
    11701 agcctttcaa cgcccatgcc gaagccgcag gccggagtcg cgggaccgcc gatttcctca
    11761 ataagcccgt cgtaccttcc gccgccgcaa accgtgccct gagttccgat tttgtctgac
    11821 acaaattcaa atacagagcg ggtatagtag tcaagacctc tgacaatcat cggatttact
    11881 ttatactcaa cgcccaaagc gtcaaggtcc gccttaaagg actcaaaatg ctccttgcag
    11941 tcgccgcaaa ggctgtcaag cataatcgga actcccttag agatttcctt gcagacgggg
    12001 ctcttgcagt ccagtatgcg catggggttt ctctcgagcc tatccttgca ggtatcgcaa
    12061 agggaatcaa tgtgctcctt aaagtaagcg cgcagctttt cgagatatac ctttctgcac
    12121 tcggggcagc caatggagtt tatttcaaga tgtatgtcct caatgcccaa aagctcaaag
    12181 aaagcgtcgg caagggcgac aatctcagcc tccgcgctgg agtcggaagc gccgaatgtc
    12241 tcaacgccga actgatggaa ttccctgagc ctgccggcct gaggcttttc atatcggtag
    12301 catgaaacct tgtatacgaa tttctgcggc gcgggctcat tgaaaagacc gtgctcaaca
    12361 taagccctgg caacgccggc tgtgccctcg ggccttaaag ttatagacct tcccgaaagg
    12421 tcgttaaagg tatacatctc cttttggacc acatccgtag tctcgcccac ggagcggtgg
    12481 aacagctccg tatgctcaaa gacgggtatt ctaatctcct taaagccgaa agactcagct
    12541 aaagataaga attcgtcctc aaggaattga tatttatgaa tctcagaggg gaggatgtcg
    12601 tgtgtgcccc taggagcttt cgtaagcagt gccattcaat cagtccttta aatcaattct
    12661 taagttattt ttacaaagac taaatataaa gatacaggaa aaaaatgaaa aataaaagat
    12721 gaatttaaaa atttatgtca aatacaaaaa agtagcggca gggcagtacc ctttgccgtt
    12781 acaaaagcta agataaatca ttttattaag cattttaaaa attgtttgcc ttgcggtctt
    12841 tccgcaaaag caaacaattc cttaaagtta tttgtttttc ggatttacaa ggtcgcgcca
    12901 gtcaaggtcg cctctctcaa gacctcttac cagaacctcc gccgtagcga tgtttgttgc
    12961 tacgggaatg ttgtgcacat cgcaaagatg caggagattc atctcgttcg gctcgcccgg
    13021 ctttgcggtc agcgggtctc tgaacattaa aagaaggtca atttcgttgc aggcgattct
    13081 tgccgctatc tgctggtcac cgccctgaga gccgcttaaa aagcgctgta tttcaagacc
    13141 ggtagcctcc gcgacaagct tgcccgtggt accagttgcg caaagcgaat gacggcttaa
    13201 aattccgcag tatgctatgc agaattgaat cataagctcc tttttagcgt cgtgagcaat
    13261 caatgctata ttcatatatt aaccccattt cggcaggatg ttttttccta taactttaaa
    13321 atagaataac acaaaaacat atgtattgca atacttccaa agttttgccg cgattttttt
    13381 tgagcagttt tgcggattag tatgtttctt tttacttaaa gcatgtacaa aattatcaaa
    13441 attattatca ttttggatta taaggagctt tctttaaata aagattaaat tcacagcatt
    13501 tttgactcaa aataactcat tacaaaaaag gataaccgta ttctcggcta tcctttatat
    13561 ttaataagtt cctttacggg cagcggcact gattcgccct ctatcctttt tgctattctt
    13621 tgaaccgagg cttcaacggc gcttttgccc gatgcatttt cgccctttac gagggatacg
    13681 acagaggagt caaaaggcac aacgcctaaa agtcttgcgc aaaccgtgtc cattatatcg
    13741 tcaagggtaa aaaaatcttc tttagagcag gggaggcgat ttacgctgaa tttattaacg
    13801 ataaggctaa tatcctcaac gccttcactg aacaggaatt cggacgctgc tgcggctgcg
    13861 gatacggcga ttttgtcggg caccgttact attatcgcag cgtctgacgc ggcggccgcg
    13921 attttaagcg attgacccac tcccgcggag gagtcgcaga gaatataatc aaaattcgcc
    13981 gacaaagccg aaagcatttc cttaatttcg gaaggctcaa cctgaacgcc gccctcattc
    14041 ggggcgtgaa gaaggctgag attctgcccc aaacttgaca cagcgtcctg tacccttgcc
    14101 ttgcccgttg ccgcgtccag ccagttatag tgggtcaggg aatcggcaga gagcataagc
    14161 tcaagggccc ttacgcccgc gtccatgtcg gcgagcaaaa ctctcttgcc ttgtcttgcc
    14221 aaagcccttc ctattgcggc ggtcatggtg gatttgcccg ttccgccctt gcccgaagcc
    14281 agtgttataa tccgagctat ttaaatcact cccgtccgcg cgtttaagag aatcttattt
    14341 gtagcccttg agctttaaga atctgtgaat tctctcgttg gggctcagaa gcttgctgat
    14401 tgaaaggtca tggttaagag tgtctataag gagggataca tacttagaca tatcaacctc
    14461 tgtaaaccac tcagatgcca aaagctcagg agacctgtaa acaaggtttg tggtgaatac
    14521 tctgtcaatt atgccgtcct tgtatgcctt atggaagttt gcgagaccgt cgcagaaaag
    14581 accgaatgcg gcgcagatga aaatcctttt ggccttcatg ctcttaagct tgctcgcaac
    14641 ctcaagaacc gactctccgg aggatatcat gtcgtcgact attacaacat ccttgccctc
    14701 cacattcgcg ccaaggtact catgggcaac tatcggattt cttccgtcaa cgattcttgt
    14761 atagtcacgg cgcttataga acatacccag gtcaattccc agaactgtgg agtagtaaat
    14821 gcacctgctc attccgccct cgtcggggga gataatcata agatggtcct tgtcaatctt
    14881 aaggttgtcc actgtgttta caagagcctt tatcatctga taaactgtgg tgacattttc
    14941 aaagctctta agcgggattg cgttctgaac tctcgcgtcg tgagcgtcaa aggtgatgat
    15001 gttgtcaacg cccattgtgt ggcaaagctc ctgcagagct atggcgcagt caagcgattc
    15061 tctgcttgag cgcttgtgct gtctgccctc ataaagcatg ggcatgatga cggtaattct
    15121 gtgcgccttg ccggctactg cggcgatggc tctcttgaga tttgcatagt ggtcgtcggg
    15181 gctcatgggt actaccatgt cgtacatctt gtaagttacc ccataattaa aaacatccgt
    15241 gaggataaaa aggtcatatc ctctgacgga ttcagtaata agcgcttttg cttcacccga
    15301 accaaaacgg ggaaatttta catctatgat atagctgtcc ctctgatagc ccgcaaatgc
    15361 aatggtagtc ttatgctcgc tctccctgtt tgcacgccat tcaacgatat acttgtcaat
    15421 ttttgccgcc agttcctcgc agccgggcgt agcgattatt cccaacggac caaagggtat
    15481 agtggtaaaa tcttcggtat aagtaggcat ttcagatatc ctccgtaaag catttttaac
    15541 aacctgtgtc ggagccgata atatgaagta agttttgaga gaattttgtc cgctccgtcg
    15601 ctacttatat tctacaacaa atacttttat ttgcaagagt aaaaagagat agaagattgc
    15661 aaattttaat ttcgtaagtt tgtataaaat tttgaaaaga gcttcccgta tttatttttg
    15721 gcaaaattta gctgctaatt catgttccgt aaaaattaaa gccgcacttt gtgcggcttt
    15781 ttattatttc actttttccg cctcttcggc gataaccttc tttaagattt ctaccgccct
    15841 ctttaagtcc tcaaagggga tattcagcgg cggcaggagt cttacctttg tctttgccgt
    15901 tagtaccaga actccgttct ctacagcctt ctgagccaca tccgcgggag gagcctcggt
    15961 ttcgattccc accataagac ccaagccgca aacggatttt acgcc
//
LOCUS       DAONVQ010000029        16002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2716_length_16002_cov_4.643256, whole genome shotgun sequence.
ACCESSION   DAONVQ010000029 DAONVQ010000000
VERSION     DAONVQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16002
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2716_length_16002_cov_4.643256"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /gene="rplT"
                     /locus_tag="PLD98_03140"
     CDS             complement(<1..234)
                     /gene="rplT"
                     /locus_tag="PLD98_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQA05279.1"
                     /translation="MARVKGALATRKRRNKVLKLAKGYYGSKSKLFRTAKQAVMKSGN
                     YAYVGRRLKKRDFRRLWITRISAACKLNGMNYST"
     gene            complement(292..489)
                     /gene="rpmI"
                     /locus_tag="PLD98_03145"
     CDS             complement(292..489)
                     /gene="rpmI"
                     /locus_tag="PLD98_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HQA05280.1"
                     /translation="MPKIKTNSGAKKRFKLSKNGKPIRAHANKSHILTKKSTKRKRGL
                     RKTTVADTTNVKQIKRLIPYK"
     gene            complement(580..1086)
                     /gene="infC"
                     /locus_tag="PLD98_03150"
     CDS             complement(580..1086)
                     /gene="infC"
                     /locus_tag="PLD98_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQA05281.1"
                     /translation="MQINREIRDKEVRVVSENGEQLGIMLIEEALKIAASYDLDLVKI
                     APQAKPPVCKIMDYGKYRFEQAKREKEAKKNQHSIEIKEVQLSLNIDKHDLETKVGHA
                     IRFLKAGNKVKVVIRFRGREMAHPERGVDIMQQFASMCGDICTIEKQPNIDGRNMIMF
                     LVAAKPGK"
     gene            complement(1345..2163)
                     /locus_tag="PLD98_03155"
     CDS             complement(1345..2163)
                     /locus_tag="PLD98_03155"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQA05282.1"
                     /translation="MEYKIFANHAHVFDAERRPNGTVQELLKLMDNCGISKAVCFAPF
                     KGQTSDPNIDPVEWLAKEIENEPRLVGFGTIDFDRDDIEKQVERIAELGFKGIKVHPA
                     AQEISIVGEKARRMYEVAQEKGLFLSFHTGIHWHRISDYHPILFDEVAFDYPMLQFSL
                     EHIGGYHFFKDALAVMCNTKRPIPRVYAGWTSITPPDNNPPSSWTISDEELRTVIYQT
                     GNEYSIFGLDFPYNREEYTMKAIERIINLDIPEEAKRGVLGENLARVLDVSLDD"
     gene            complement(2189..3427)
                     /locus_tag="PLD98_03160"
     CDS             complement(2189..3427)
                     /locus_tag="PLD98_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQA05283.1"
                     /translation="MTDIMEAKKLIVKAGLRLLETGLIARTWGNVSCRVSDTHFAITP
                     SGRAYNTLTSDDIVLVKIDDLSYEGDIKPSSEKGVHAEVYKHRPDINFVIHTHQLNAS
                     MISVLRSDINFVPEKSAAIIGDHIPVSSYGLPSTKKLRDGVTETLKRSDSKAILLANH
                     GAVCMGVDYEDAFKVAEELEKVCEDYVRRRFSLVTGKIAETFESLNEYIAEKSALSAE
                     KAKEIRPYNSEREGEFIKLSPVNGGDIIRVNTVTEKAVAPNSDYPPEIYLHLAVFNKR
                     KDVNAIVHSKKPEILAVSKCCKVLKPFLDDFAQIAGISARSAEFDPNDALKTSKKVVK
                     KLKGRNSVLLKDNGMLCVAGNNSDAQAVEMVAEKNAKAYIASKLFGKNNVIAPHEALL
                     MRFIYKAKYSKKAQEGSNGK"
     gene            complement(3505..4980)
                     /locus_tag="PLD98_03165"
     CDS             complement(3505..4980)
                     /locus_tag="PLD98_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQA05284.1"
                     /translation="MENKFAITTYPNAAEITKQLDELIKKPIYTIKPEALKAYEEEYY
                     EKKCKKSKEMIEKAKEIIPGGVQHNLAFNYPFPLVFTKAEGAKLYDIDGNEYLDFLQA
                     GGPTVLGSNPPEVREQVIDLLKTCGPSTGLFHEFEYKLAKKISDNVETVQMFRMLGSG
                     TEACMAAARVARLATKKKNIIKMGGAYHGWSDQLAYGIRIPGSKFTQANGVPLHTFKY
                     TQEFFPNDLDDLERKLRFNMLRGGTAAVFIEPVGPESGTRPVDFDFNKGVERLCRKYG
                     ALLVFDEVVTGFRIGMSCAQGYFGVSPDLTIFGKAIAGGYPGAGGLGGKKEYMKYLGA
                     GLDGSTKIKKALVGGTMAATPISCVAGYFALEAIEQTNACYKAGLMGDRLTQGLQKLI
                     KKYGLPFVAFNQGSICHLETVGTMHFAINWMRPWEIPHVLSETSIRKKEMEHMGAAYM
                     AEGLVTLAGSRLYTSAAYTPELIDEALKRFDKVFANVAMKK"
     gene            5297..6022
                     /gene="nagB"
                     /locus_tag="PLD98_03170"
     CDS             5297..6022
                     /gene="nagB"
                     /locus_tag="PLD98_03170"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HQA05285.1"
                     /translation="MRVVILENASDIAVQAGNIFVELLTDKPNAVLGLATGATPVPTY
                     KYLIDCYKQGKVSFKDAVSFNLDEYVGLDRNNVNSYYYFMMENLFNHVDMRKDKVHVP
                     NGVAENLEEECARYDAMIEEHGGIDVQLLGIGTNGHIGFNEPSDAFSDGTYITDLAQS
                     TIESNKIYFTNGDMPTKAITMGIGSIMKAKKIILIATGISKAKAIKAMIEGEVTPACP
                     ASILQKHDDAVIFLDKDAASLLA"
     gene            complement(6068..6805)
                     /locus_tag="PLD98_03175"
     CDS             complement(6068..6805)
                     /locus_tag="PLD98_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQA05286.1"
                     /translation="MWNQNEKNFYVFGHRGVKALYPENTMLSFQKAYEMGVDGIEFDV
                     RATKDGELILMHDETVDRTTDGSGLVRDFTFAELRKLDAGVRFGEEFKGNRVPSLTEL
                     FDSVKGTSLLLNVEIKDYEFDVVDKTIDLLFKYGFDERFVITCFDAKITTYTHEKYGV
                     KVQGFPDSYMKNFEESARRHFYSVGIAMKDLTKELCDSYKKAGIDPWCWSPDDEDGVR
                     HMIECGSTLATCNNPLPALKIRGEINK"
     gene            complement(6810..8081)
                     /locus_tag="PLD98_03180"
     CDS             complement(6810..8081)
                     /locus_tag="PLD98_03180"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HQA05287.1"
                     /translation="MDFEKLLNGIDNCPCGKAHKCDIEKIIIKKDAIKELKRLLSSYS
                     NILLVADTNTYEICGERVKAILGDAVKDELIYPGVPLLVPDENAIKALNDSVKSETDI
                     IIAVGSGVINDLCKLISYRRSRPYCIVATAPSMDGYASSVSAVIEGNMKITYAAHSPK
                     FIIADLDVLKNAPYEMIQAGFGDTIGKYSALNDWLLSKIVNGEYFCETVYDMVYSVLQ
                     KTAEIGPRLKDRREEDIKLLFESLVIVGIAMAYVGNSRPASGSEHHLSHFFEVVGLLN
                     NEPYLTHGICVAGSAVITQRLREEIIEIQEFESGFKFDREKWERAVRSIYSKAADGVI
                     DLQDKTGYYKRDFASIYKEKWEEIISVFNKAPSSEYMLDLLNSAGISFSEFNSVYSPE
                     KIRNAVFFAKDLKDRYTVLWLYFAMKYKFEE"
     gene            8227..8415
                     /locus_tag="PLD98_03185"
     CDS             8227..8415
                     /locus_tag="PLD98_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HQA05288.1"
                     /translation="MANLTAEELKALEDQLNMEQLLIKKYRSYASMTADPQIKQTCEN
                     IANMHKQHFDKLMGHLCC"
     gene            8440..8712
                     /locus_tag="PLD98_03190"
     CDS             8440..8712
                     /locus_tag="PLD98_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HQA05289.1"
                     /translation="MNNIPMTDQEIMTDVLVSQKHITGVYNTFANECKNKQLKDDMMC
                     ILNEEHTIQHNVFTEMEKRGWYAPPAAEQQKINEAKTKFQNISTSL"
     gene            complement(8769..9776)
                     /locus_tag="PLD98_03195"
     CDS             complement(8769..9776)
                     /locus_tag="PLD98_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQA05290.1"
                     /translation="MTVEAKSKGKKAFKTVIIALLVIAAALLIFSYFTSRETSVPVAA
                     YNGKNKYITPTGQAMVSAHRSGGGIFPENTFLAFKSCVESDTFNTDIFEFDLHLTADN
                     VLVLLHDDTLDRTSNAEEHFGEKDVKPSAKTYAELRELNMGESFKDDNGKQPYKGLRG
                     KDIPDDLRIIRLEDVLDYLCANGDYHFIIEIKDSGELGKKAADELYKVLKSRNLIEKT
                     IVGTFHGEVTKYIDEAYPDMIRSASIIEVISFYFSSLFGIKKNPEKIKFDALQIPPKA
                     AMLNLSTTRFINYAHSHDIAVQYWTVNDKEQIKALNEKGADAIISDVPDIAYAIIHAN
                     D"
     gene            complement(9810..11234)
                     /gene="cysS"
                     /locus_tag="PLD98_03200"
     CDS             complement(9810..11234)
                     /gene="cysS"
                     /locus_tag="PLD98_03200"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQA05291.1"
                     /translation="MKLYNTLTRQKEEFIPLEEGKVKIYACGPTVYNYIHIGNARPLC
                     VFDVLRRYFIFSGYNVTFVQNFTDIDDKIINKANEMGVESIEVSEKFIEEYKKDAKGL
                     NIMTADVHPKVTENIDEIISIIETLIEKGYAYEVEGDVYFSTNKFEEYGKLSHQPLEE
                     LEAGARISVGEKKAEPMDFALWKAAKPGEPAWDSPWGKGRPGWHIECSAMARKYLGQT
                     IDIHCGGQDLIFPHHENEIAQSECCNGVPFARYWLHNGYINVDNVKMSKSLGNFFTVR
                     DVAEKYGYEPIRYLMISSHYRSPINYSKEAIEQCTAALSRLYNCRENLDFAIENAETE
                     KSDEAKISEVKAALNEYRQKFIDSMEDDFNTADALGALFEMVRYINKEVLAVGAAKDV
                     LNLASDAFKELCDILGLLYEKAEKAAETDPLIDTINAMIEERTQARKEKNWAKADEIR
                     NKLREMGIVLEDTPQGVKWHKVQE"
     gene            complement(11249..11899)
                     /gene="cysE"
                     /locus_tag="PLD98_03205"
     CDS             complement(11249..11899)
                     /gene="cysE"
                     /locus_tag="PLD98_03205"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HQA05292.1"
                     /translation="MFDRLREDICCVKERDPAARSAVEVLLLYPGLKAIRAHRRAHWF
                     YNRGMFFIARWISQRAARKTGIEIHPAAKIGRRFFIDHGCGVVIGETTEIGDDVTLYQ
                     GVTLGGTGKDTGKRHPTIGNNVMIGAGAKVLGPIKIGDNTNIAAGAVVLKDIPPNSTA
                     VGVPARIVKRDGKRVDDSDPRNLKDPIAQEIERLEARITCLRKAIDAMSEKMEEQC"
     gene            complement(12048..12491)
                     /locus_tag="PLD98_03210"
     CDS             complement(12048..12491)
                     /locus_tag="PLD98_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HQA05293.1"
                     /translation="MKKAKKPKKIKKSDNKIKNTRLRSLINPLSCIGFSIVVFILFLL
                     LFAFSMVKSNVSFKILPVFLTVSMLASSFISGFYAAKRLNLRGMFAGFLASLPIVIAT
                     IVCSLIVNNGSTRLLLYLVCLAAMVAGAIGGIFSANTRPAKRRIG"
     gene            complement(12611..12686)
                     /locus_tag="PLD98_03215"
     tRNA            complement(12611..12686)
                     /locus_tag="PLD98_03215"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12651..12653),aa:Lys,seq:ttt)
     gene            complement(12700..12951)
                     /gene="secG"
                     /locus_tag="PLD98_03220"
     CDS             complement(12700..12951)
                     /gene="secG"
                     /locus_tag="PLD98_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQA05294.1"
                     /translation="MPAYQIIISIFLIILSALLTVVILLQQNREAGLSGAITGAADSF
                     LGKNKRRSAEAKLAQITKIVAICFFSLALIASVLITLFS"
     gene            complement(13175..13825)
                     /locus_tag="PLD98_03225"
     CDS             complement(13175..13825)
                     /locus_tag="PLD98_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05295.1"
                     /translation="MGSVHKQIIVSILLLSLLFGFCSCSKSGKDETTTKRESDFKVSE
                     FDKANDEDMKPYLSYVESCVKDYYKAVLADKSRLPNLPLPMSANLREYLKIKFDYETK
                     LEKKNEKDKKYAGGAFNVFDYEIQDNRLVCIVVAEVNYRYSAEEAPIYMVEEVQVVIE
                     NPQSPTIIDWYVNDPESFDSLVRGEDLLLSESENLLINQDFEKIKEKAKDLIASIK"
     gene            complement(13844..14665)
                     /gene="rhaD"
                     /locus_tag="PLD98_03230"
     CDS             complement(13844..14665)
                     /gene="rhaD"
                     /locus_tag="PLD98_03230"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HQA05296.1"
                     /translation="MSSIIDAGFVKAFAKLCDDAYNKGWHERNGGNLSYRIKDEELKP
                     YLSQLSFDGEFKPIGVTVENLKNEFFLVTGSGKYFQNVKADIENNVAIIEIDESGSAY
                     RIVWGLASGGVPTSELPTHLINHSIKKEKTNGLHRVIMHSHPANTIALTFVLPLDSKV
                     FSRELWEMMTECPIVFPRGVGVLPWMVPGSADIALKTAERIKEHDVIIWAHHGVFCSG
                     ADFDETFGLMHTVEKAAEILVKVLSCGGKRQTMSADDISSLSGPYKVNINTDFLK"
     gene            complement(14691..15941)
                     /locus_tag="PLD98_03235"
     CDS             complement(14691..15941)
                     /locus_tag="PLD98_03235"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HQA05297.1"
                     /translation="MAGDRYNHAKKVYADLGVDTDKALETLQKVKISMHCWQGDDVSG
                     FEGAGELSGGIAATGNYPGKARTPEELMSDIDMAFSLIPGKHKLNLHAIYAVTNGEKV
                     PRNKLQPVHFEKWVEFAKERGLGLDINPTLFAHEYASDTLTLSHPDKKIRDFWIEHCK
                     AMRKVGEYFGKELGQPCLNNIWIPDGYKETPADRKGPRERLKESLDEIFSEKYSRDYL
                     YDSVESKLFGIGVESYTVGSHEFYLSYASKNDVLCLLDSGHFHPTEVISDKLSSLLLF
                     HDKIALHVTRGVRWDSDHVVTLDNELIEIAKEIVANHALDRVLIGLDFFDASINRIAA
                     WVIGMRNMQKALLYALLMPLKEMKLLQEKGDFTRMLMMNEEFKTMPFSDVWDRFCEMN
                     NVPVRNEWYKAVEEYEKNVLSKRQ"
BASE COUNT         4164 a         3717 c         3401 g         4720 t
ORIGIN      
        1 ggtagaataa ttcataccgt tgagcttgca agctgctgaa attcttgtaa tccaaagtct
       61 gcggaaatca cgcttcttaa gccttctgcc tacataagcg tagttgccgg acttcataac
      121 tgcctgcttc gcagttctga acagcttgct cttggagccg tagtagccct ttgcaagctt
      181 taatacttta tttcttctct tacgagttgc taatgcacct tttactcgag ccatgttagt
      241 acctccttga atttgaagcc tttaaaagga ttgccgattt atacggaaac cttatttata
      301 aggaataaga cgcttaatct gctttacatt ggttgtgtca gcaacggtag tcttgcgcag
      361 tcccctctta cgctttgtag acttcttggt aagaatatga gacttattgg catgagctct
      421 gatgggcttg ccattctttg aaagcttaaa acgcttttta gctccgctgt ttgttttaat
      481 cttaggcatt tttgtttcct cctgtataga aattctgaat ttgcgaacct gaagacgaaa
      541 taaccggaac gccgggtcat cgtatcagta accctgcaat tacttgccgg gctttgctgc
      601 aacaaggaac attatcatat tgcggccgtc gatattgggc tgcttttcta ttgtacaaat
      661 gtctccgcac atagaagcga actgctgcat aatatccacg ccgcgttcgg ggtgagccat
      721 ttctctgcct ctgaagcgta ttacgacctt tactttattg ccggctttga ggaatctaat
      781 tgcgtgaccg acttttgttt caaggtcatg cttgtcgata ttgagagaca gctgcacttc
      841 tttaatctca atggaatgct gattcttttt ggcttccttt tcacgctttg cctgctcaaa
      901 gcgatatttg ccgtagtcca ttattttgca gaccggcggc tttgcctgag gcgcaatctt
      961 aaccaagtca agatcgtagc ttgcggcgat tttcaaagcc tcttcaatca gcataatacc
     1021 aagctgttcg ccgttttcgg aaacaactct gacttccttg tctcggattt cccgattgat
     1081 ttgcatttgt ttgttactga tacttaaaca cctccgatta attaaaaaga gtgcggaatc
     1141 acccgcactc aaaactgcaa aacaagcagc ataaaagctg cactgcttta aagtattgac
     1201 cctgcggaga actccttaag gtgagaggaa atattaattt cactctgctt tgttgtacag
     1261 atagtttaac agcttgcaaa atatttgtca agctattttt tcacataaaa ttatatgcac
     1321 aaattttaaa ttatgcaacc aaatttaatc gtcaaggctg acatcgagaa ctcttgcgag
     1381 gttctcgccc agaactcccc tctttgcctc ctcgggaata tcgaggttaa ttattctctc
     1441 gattgccttc attgtgtatt cctcgcggtt gtaggggaaa tcaagaccga atatgctgta
     1501 ctcattgccg gtctggtata tgacggttct taattcctcg tcgcttatag tccagctcga
     1561 aggaggattg ttgtcgggcg gggttatgct tgtccagcca gcgtaaactc tgggaatagg
     1621 tctttttgtg ttgcacatga ctgcaagggc gtctttgaag aagtggtagc cgcctatatg
     1681 ttcaaggctg aactgaagca taggatagtc aaatgctacc tcatcgaaca aaatggggtg
     1741 ataatcggat attctgtgcc agtgaatgcc tgtgtggaag ctgaggaaaa gacccttttc
     1801 ctgagcaacc tcgtacattc tgcgggcctt ttcgccaaca atgcttattt cctgtgcggc
     1861 ggggtgaacc ttaattccct taaagcccag ctcggcaata cgctcaacct gcttttcgat
     1921 gtcgtccctg tcaaagtcga tggtgccaaa gcctacgagt ctcggctcat tttcaatttc
     1981 cttggcaagc cattcgacgg ggtctatgtt tgggtccgaa gtctgaccct taaaaggggc
     2041 aaagcatact gccttgctga ttccgcagtt atccatgagc tttaaaagct cctgaacggt
     2101 tccgtttggt cttctttcag cgtcaaaaac atgggcatgg tttgcaaaaa tcttgtattc
     2161 catagagtct cctttatgct aaagctattt atttgccatt actgccttct tgagctttct
     2221 ttgagtactt tgccttgtag ataaacctca tcagcaaagc ctcatggggc gcgattacat
     2281 tattcttgcc gaagagcttt gaggcgatat acgccttggc gttcttttcg gcaaccattt
     2341 caaccgcctg agcgtcgcta ttattgcccg ccacgcagag catgccgttg tctttaagca
     2401 gtacgctgtt tctgcccttt agctttttta ctaccttctt agaggttttt aaagcgtcat
     2461 tcgggtcaaa ctcagcggac cttgccgata tacccgcaat ctgggcaaag tcatcaagga
     2521 agggtttaag gaccttgcag cacttggaca cggcaagtat ctcaggcttt ttggagtgaa
     2581 caatggcgtt tacatccttg cgcttattaa atacagcaag gtgcagataa atctcgggcg
     2641 gatagtcgga attgggagcg accgcctttt ctgttaccgt atttaccctg ataatatctc
     2701 cgccgtttac gggtgagagc tttataaact ccccttccct ttcggaatta taaggcctta
     2761 tttctttagc cttttcagcg cttaaagccg atttttcggc tatatactca tttaaagatt
     2821 caaaggtttc agcgattttg cccgtaacca gactgaacct gcggcgtaca taatcctcgc
     2881 agaccttttc aagctcctct gcaaccttaa aggcgtcctc ataatccacg cccatgcata
     2941 ctgcgccgtg gttagcaaga agtattgcct ttgaatctga gcgtttaagt gtctcggtta
     3001 cgccgtcacg cagctttttt gtgctcggca gaccgtagga tgaaacggga atatggtcgc
     3061 cgattatcgc agcgctcttt tcgggcacaa agttaatgtc cgaccttaaa acgcttatca
     3121 ttgaggcatt aagctgatgg gtatgaatga cgaaatttat gtcgggtcgg tgcttgtaaa
     3181 cctcggcatg aacgcccttt tctgaggagg gcttaatatc tccttcatat gaaaggtcat
     3241 cgattttgac aagcacgatg tcgtcggaag ttagggtatt atatgccctg ccgctcgggg
     3301 tgatagcaaa atgggtgtcg ctgacgcggc agctgacatt gccccaggtt ctcgctataa
     3361 gacctgtttc caggagtctg agacctgctt tgactatcag ctttttggct tccattatat
     3421 cagtcatgaa aatcaccttg atattataaa ttagaaaaaa acttcaagta aaggggttgg
     3481 tttgcgccaa cccctttaaa aggtttattt cttcattgcg acatttgcaa ataccttgtc
     3541 aaatctctta agggcttcgt caatcagttc gggagtatat gcggcgcttg tgtaaagcct
     3601 gctgccggca agagttacaa gaccctcagc catatacgcg gcgcccatgt gctccatttc
     3661 cttcttgcgg atgcttgttt cggaaagtac atgcggaatc tcccacggac gcatccagtt
     3721 gattgcaaag tgcatggtgc cgactgtttc aaggtggcag attgaaccct ggttgaatgc
     3781 cacgaagggc agaccgtact tcttaatgag cttctggaga ccctgagtga gtctgtctcc
     3841 cattaatcct gccttgtagc atgcgttggt ctgctcgatt gcttcaaggg cgaagtagcc
     3901 ggcaacgcag gaaataggtg tcgctgccat tgtgccgccg acgagagcct tctttatctt
     3961 tgttgagccg tcgagacccg cgccgagata cttcatatat tccttcttgc cgccgagacc
     4021 gcctgctccc ggatagccgc ctgctattgc tttgccgaaa atcgtaaggt cgggcgatac
     4081 gccaaaatag ccctgagcgc aggacatacc gattctaaat cctgttacaa cctcgtcgaa
     4141 tacaagcaat gcgccgtact tgcggcatag acgctcaacg cctttgttga aatcaaaatc
     4201 aacgggtctt gtgccgcttt cgggacctac gggctcaata aagaccgctg cggtgccgcc
     4261 tctgagcata ttgaaacgca gctttctctc aaggtcgtca aggtcattgg ggaagaattc
     4321 ctgagtatat ttgaatgtgt ggagcggaac accgtttgcc tgtgtaaact ttgagccggg
     4381 gattctgatg ccgtaggcaa gctggtcgct ccagccgtga tatgctccgc ccatcttgat
     4441 tatgttcttc ttctttgtgg caagccttgc tactctggct gctgccatgc aggcttcggt
     4501 accggagccg agcatgcgga acatctgaac ggtttcaaca ttgtcggaaa tcttcttagc
     4561 gagcttatac tcaaactcat ggaaaagtcc tgttgaagga ccgcaggtct tgagcaaatc
     4621 aataacctgc tctcttactt caggaggatt ggaaccgagg acggtagggc cgcctgcctg
     4681 caaaaagtca agatactcat tgccgtctat gtcataaagc tttgcgccct ctgccttagt
     4741 aaaaacaaga gggaaaggat agttgaacgc aaggttgtgc tgaacgccgc cggggataat
     4801 ctcttttgcc ttttcaatca tctctttgga ctttttgcac tttttctcgt agtactcctc
     4861 ttcgtaggcc ttcaatgctt ccggcttgat tgtataaatc ggcttcttta taagctcatc
     4921 aagctgtttg gtaatctcag ccgcattggg ataggtggtt atcgcaaact tgttttccat
     4981 tgtctaaacc tcctgagcgt gatttatgaa aatagattaa tgaaacggat aagaaataag
     5041 cctcgaaatg ctctgcgcaa tcaagacaat tatagcattt atacaaataa tgtcaatcaa
     5101 accaaagatt atccacaact aattaacaaa aagataatct attatttaca attgtattta
     5161 tacaaaattt acttaatttg ctaaaaaaat gtgtattttg ataattctcg caaaaagtga
     5221 aagaaattgt tcacttcttg agtttttatg ttataatctc atagaacttg gaaaaacaaa
     5281 aatcggaggg aaatcaatgc gtgtcgttat ccttgaaaat gcatcagaca tcgccgttca
     5341 ggcaggcaat atatttgtgg agcttttgac ggacaagccg aatgcagttc tcggacttgc
     5401 aaccggcgca acacctgttc cgacctacaa ataccttatt gactgctata agcagggcaa
     5461 ggtcagcttt aaagacgctg tttcattcaa tcttgacgag tatgtgggcc ttgacagaaa
     5521 caatgtcaac agctactact actttatgat ggaaaacctc tttaaccatg ttgacatgag
     5581 aaaggacaag gttcatgtcc ccaacggcgt tgcagaaaac cttgaggaag aatgcgcccg
     5641 ttatgacgct atgattgagg agcacggcgg aattgatgtt cagctcctcg gcatcggcac
     5701 caacggtcat atcggcttta acgagccttc cgacgcattc agcgacggaa cttatattac
     5761 ggaccttgca cagagcacga ttgaatccaa caagatttac tttacaaacg gcgatatgcc
     5821 cacaaaggca attactatgg gaatcggctc cattatgaag gcaaagaaga taatcctgat
     5881 agccacaggc atatcaaaag ctaaggcaat caaggctatg atcgaaggag aggtcacacc
     5941 tgcctgccct gcttcaattc ttcaaaagca tgatgacgcg gttatcttcc ttgacaaaga
     6001 cgctgcttcc ctcctggcat agtattaaaa ataatttcag ccctgtactt tcatacaggg
     6061 ctgtttttta tttattaatt tcgccgcgaa tttttagtgc gggcaaggga ttgttgcatg
     6121 tggcaagagt tgagccgcac tcaatcatgt gacgcacgcc atcctcatcg tcggggctcc
     6181 agcaccatgg gtcaattccc gcttttttgt agctgtcgca aagctctttt gtaaggtcct
     6241 tcatcgcaat gcctacgctg taaaaatgcc gccttgcgct ttcctcaaaa ttcttcatat
     6301 agctgtcggg gaaaccctgc actttaacgc cgtatttctc gtgggtatag gtagtaatct
     6361 ttgcgtcaaa gcaggttatt acaaatcttt cgtcaaagcc gtacttaaac agaaggtcta
     6421 tggtcttatc cacaacatca aattcatagt ccttaatctc aacattaaga agaaggcttg
     6481 tccccttaac agaatcaaaa agctctgtaa ggctcggaac cctgttgccc ttaaattctt
     6541 cgccgaagcg tacgcccgca tcgagttttc taagctcagc gaaagtgaaa tcacgaacca
     6601 agccgctgcc gtcggtggtt ctgtccaccg tttcatcgtg catgagaata agctcaccgt
     6661 ctttagtggc tctaacatcg aactcaatgc cgtcgacgcc catttcataa gccttttgga
     6721 atgagagcat ggtgttttcg gggtataaag ccttaacgcc ccggtgtccg aaaacataaa
     6781 aattcttttc attctgattc cacataaagt tactcctcaa atttatactt catcgcaaaa
     6841 taaagccata aaaccgtgta tctgtccttt aaatcctttg caaagaaaac agcattccta
     6901 attttttctg gtgaatatac gctgttaaac tcactgaatg agattcccgc gctgtttaac
     6961 aagtcaagca tatattcgga acttggggct ttattaaaaa cggagattat ttcttcccac
     7021 ttctctttat aaatgctcgc aaagtccctt ttatagtagc ccgttttgtc ctgcaaatca
     7081 ataactccgt ctgctgcctt tgaataaatc gacctgactg ccctctccca cttttctctg
     7141 tcaaacttga aaccgctttc aaattcttga atttcaataa tttcttctct caatctttga
     7201 gtaatgaccg cagagcctgc tacgcagatg ccatgtgtaa gataaggctc attgttcaaa
     7261 agaccaacaa cctcaaagaa atgggacaaa tgatgctcgc tgccagacgc ggggcgggaa
     7321 ttgcccacat aagccattgc tatgcccaca attacaaggc tctcaaaaag gagctttata
     7381 tcctcctccc gcctatcttt aagtcggggc cctatctcag ctgttttttg aagcacggag
     7441 taaaccatat cgtatacggt ctcgcaaaaa tactctccgt ttactatctt gcttaaaagc
     7501 cagtcattca gcgcggaata cttgccgata gtatcgccaa agcctgcctg aatcatctca
     7561 tagggggcat tttttaatac atcaaggtca gcgattataa atttaggcga atgggcggcg
     7621 taagttatct tcatatttcc ctcaatcaca gcggagacgg atgatgcgta gccgtccatt
     7681 gagggcgctg tggcaacaat gcagtaaggt cttgaccgcc tgtagcttat aagcttgcat
     7741 aaatcgttta taacccctga acctaccgca ataattatat ctgtttcgga ctttacgctg
     7801 tcattcaaag cctttatggc gttttcatca ggcacaagca gcggcacgcc cgggtatatc
     7861 agctcatctt ttaccgcgtc acctaagata gcttttaccc tttcgccgca gatttcatag
     7921 gtgttagtgt ccgccacaag caaaatattt gagtaggagc tcaaaagcct tttaagctcc
     7981 ttaatagcgt cttttttaat tattatcttt tcaatatcgc atttgtgcgc ttttccgcat
     8041 gggcaattgt caatgccatt cagcagtttt tcaaaatcca taataatcac tttccattag
     8101 gtttaattac agtttactcc cgtcttaatt taaattcaat aaggctgaat atacaattta
     8161 gttttgataa taatactttt gtacaaggtg agattcaccg caataaaaac acaaggagag
     8221 ttgaatatgg caaatcttac tgctgaagaa ttaaaagcac ttgaagatca gctgaatatg
     8281 gaacagctgc ttattaagaa atacagaagc tacgcaagca tgaccgcaga cccgcagatt
     8341 aagcagacct gcgagaatat cgcaaatatg cacaagcagc attttgacaa gctgatgggc
     8401 catctctgct gctaaaaaaa ttaagaaagg atgagtattt tgaataatat cccgatgacc
     8461 gaccaggaaa tcatgaccga tgttttggtt tctcagaagc atattacagg cgtttataac
     8521 acctttgcca atgagtgcaa gaacaagcag cttaaagacg acatgatgtg cattcttaac
     8581 gaggagcaca caattcagca caatgtgttc accgagatgg aaaagcgcgg atggtatgcg
     8641 cctcccgctg ctgaacagca gaagattaac gaagcaaaga caaagttcca gaatatctcc
     8701 acaagcctct aaatttattc tgaattaaaa aacagctctg aatccagagc tgttttttat
     8761 tgttttattt agtcattagc gtggattatc gcgtaggcaa tatcgggaac atcggatatt
     8821 atcgcgtccg cgcctttttc gttaagcgcc tttatttgct ccttgtcatt tacggtccag
     8881 tactgaaccg caatgtcgtg ggagtgggcg tagtttataa atcgggttgt gcttaaattg
     8941 agcatcgcag cctttggcgg gatttgaagc gcgtcaaatt taatcttctc aggattcttt
     9001 ttgatgccaa aaagcgagga gaaatagaaa gatataacct caataatgct ggctgagcga
     9061 atcatatcgg gataagcttc gtctatgtac tttgtaactt cgccatggaa ggtgccgacg
     9121 attgtttttt ctataagatt cctcgattta agaaccttgt atagttcatc cgcagctttc
     9181 tttccaagct cgcccgaatc cttaatttca ataataaagt gataatcacc gttagcgcaa
     9241 aggtaatcga gcacatcttc gagcctaata attcttaaat cgtcgggaat gtcttttcct
     9301 ctaagacctt tatagggctg cttgccgttg tcgtctttaa acgactcgcc catattcagc
     9361 tctctgagct ccgcataggt ctttgcggag ggctttacat ccttttctcc gaaatgctcc
     9421 tcggcgttgc ttgttctgtc gagggtgtcg tcgtgaagta gaacaaggac attgtctgcg
     9481 gttaaatgga ggtcaaactc aaaaatgtcg gtgttaaagg tgtcgctctc aacacagctc
     9541 ttaaaggcta agaatgtatt ttcggggaaa attccgccgc ctgagcggtg ggcggaaacc
     9601 attgcctgcc ctgtgggagt aatgtactta ttcttgccgt tgtaggcagc tacgggaacc
     9661 gaggtttctc tcgaagtgaa ataggaaaaa atgagcaaag ccgctgcgat tacaagtaaa
     9721 gcaataatca cggttttaaa cgccttcttt cccttggatt tggcttctac agtcaattaa
     9781 agcacctccg attttaagct ctcataactt tattcctgaa ccttatgcca ctttacacct
     9841 tggggtgtat cttcaagcac gatgcccatt tccctgagct tgtttctgat ttcgtcagcc
     9901 ttagcccagt tcttctcctt tcgtgcctga gttctttctt caatcatcgc gttgattgta
     9961 tctatcagcg ggtcagtctc ggcggccttt tctgcctttt cgtaaaggag accgagaatg
    10021 tcgcacagtt ccttgaaagc gtcggaggca agatttagta catccttagc ggcaccgaca
    10081 gccaaaacct ctttgttgat gtatcttacc atctcaaaca gagcgccgag ggcgtcagcg
    10141 gtgttaaaat cgtcctccat tgagtcaata aacttctgac gatattcatt cagagcggct
    10201 ttgacttccg aaatttttgc ttcatcggat ttttcagtct cagcgttttc aatggcgaaa
    10261 tcaaggttct cgcggcagtt gtaaagcctt gagagagcgg ctgtgcactg ctctattgcc
    10321 tctttgctgt agtttatggg gcttctgtaa tgggatgaaa tcataaggta tctgataggc
    10381 tcatagccgt atttttcggc gacatccctt acggtaaaga aattgcccag agactttgac
    10441 atttttacat tgtcaacatt tatatagccg ttgtggagcc agtagcgggc aaagggaacg
    10501 ccgttgcagc attcgctttg ggctatctca ttttcatggt ggggaaaaat aaggtcctga
    10561 ccgccgcagt gaatatcaat ggtctgaccg agatatttcc ttgccattgc agagcattca
    10621 atatgccagc cgggtctgcc cttgccccag ggtgaatccc atgcgggctc accgggcttt
    10681 gctgctttcc aaagggcaaa gtccataggc tcagcctttt tctcgccgac gcttattctt
    10741 gcgccagcct caagctcctc aaggggctga tgtgagagct ttccgtattc ctcgaacttg
    10801 tttgtgctga aatatacatc gccctcaacc tcgtaggcat agcccttttc gataagggtt
    10861 tcgataatag aaataatctc atcgatgttc tccgttacct tggggtgaac atcggcggtc
    10921 ataatgttaa gcccttttgc gtcttttttg tattcctcaa tgaatttctc ggaaacctca
    10981 atggactcaa cgcccatttc gtttgccttg tttattatct tgtcgtctat atcggtaaag
    11041 ttctgaacaa aggtaacatt gtagcctgag aatataaaat aacgccttaa aacatcgaat
    11101 acgcaaagag ggcgggcatt gcctatatgg atataattat acacggtagg accgcaggca
    11161 tagattttaa ccttgccttc ctcaagaggg ataaactctt ctttttgcct tgtcagcgta
    11221 ttataaagct tcatattgaa tctcctgttc agcattgctc ttccattttt tcactcatcg
    11281 cgtcaatggc ttttctgagg caagtaattc ttgcttcaag acgctcgatt tcttgagcga
    11341 tggggtcctt gaggttgcgc gggtcgctgt cgtctaccct cttgccgtca cgcttgacaa
    11401 tacgagcggg aacaccgacg gcggtagaat tcggcggaat gtctttaagc acaacagcgc
    11461 ctgcggcaat attagtatta tccccaatct ttatgggacc caataccttg gcaccggcac
    11521 caatcataac attgttgccg atggtgggat gacgctttcc cgtatccttg cccgttccac
    11581 caagggtcac accctgatat agagttacat cgtcgccgat ttcggttgtt tcgccaataa
    11641 caacaccaca gccgtggtcg atgaaaaacc ttcggccgat tttggctgcg ggatgaatct
    11701 caatgcctgt ttttctcgcc gcgcgctgag aaatccatct ggcgataaag aacattcctc
    11761 tgttataaaa ccagtgagcc ctgcggtggg cgcgaatagc cttgagaccc ggatataaaa
    11821 gcagaacctc aacagcagac cttgctgcgg ggtctctttc tttgacacag catatatctt
    11881 ctcgcaaacg atcgaacata gaacactccg ttaaaatagt atcgtactta tgattatact
    11941 acaaaattta ctaaaataaa ccttaatttg caagccttat taaattttgt ttatttataa
    12001 aaacgcaaag gggtatccta ttggataccc ccgcaaaaca tataaattca gccgatccgc
    12061 cttttggcgg gtcttgtgtt tgccgagaaa ataccgccta tcgcgcccgc aaccatggcc
    12121 gcaaggcaaa caagatataa aagtaatctt gtgctgccgt tattaactat gagcgaacaa
    12181 acaattgttg caattacaat cggcagagat gctaaaaatc ccgcgaacat tcctctgaga
    12241 ttcagcctct ttgcggcata gaaacctgaa ataaacgatg aggcaagcat ggatacggtt
    12301 aagaatacag gcagaatctt aaaggaaaca tttgacttta ccatagaaaa cgcaaaaaga
    12361 agcaggaaaa gaataaatac aacaatagaa aaaccaatgc aggataaagg atttataaga
    12421 gagcgcagcc gtgtattttt aattttattg tcgctttttt tgattttctt gggctttttg
    12481 gcttttttca tccgcatcat ccctttcaaa acctagtcta atcagtcaaa aatatgcgga
    12541 caaattacaa tttattacta agcataaaaa aagagcaccc acaaaagtga gtgctgtcta
    12601 tatttaaaaa tggtgattca gcggagattc gaactccgga caccctgctt aaaaggcagg
    12661 tgctctgcct gctgagctac tgaatcacgc tgaaattaat cagctgaaca atgtgattaa
    12721 tacagaggct ataagtgcca gactgaagaa gcagatagct acaatctttg ttatttgagc
    12781 gagctttgcc tctgcggagc gcctcttatt cttaccgagg aatgaatcag cggcgccggt
    12841 gattgctcct gacaaaccgg cctcacggtt ctgctgaaga agaataacaa cagtcagcag
    12901 tgccgaaaga attattaaaa atatgctaat aatgatttga tatgctggca caatatcaac
    12961 tcctcattct tttagcaaga gggttctttt ccgtcgtgct taaaggactt cctttacagc
    13021 gcccgattag tttatcacag ctaaacgcaa aaatcaagta tcttttatat tttttcaata
    13081 ttttttgtgt ttatcacatt tagaattgta aaactgtctg ctatttggct aatatgcatt
    13141 aaaagaaaat gcgatttaga ccttcctcat gcgtttactt tatgcttgct attaaatctt
    13201 ttgctttttc tttaatcttt tcaaaatcct gatttataag caaattttca gactctgaaa
    13261 gcaggaggtc ttcgcctctt acaaggctgt caaagctttc ggggtcattt acataccagt
    13321 caattatagt gggcgactgg ggattttcga taacaacctg aacttcttcc accatgtata
    13381 ttggggcttc ttccgctgaa tagcggtaat tgacctcagc cactacaatg catacgagcc
    13441 tgttgtcttg aatttcgtag tcgaacacat taaatgcgcc gcctgcgtat tttttatcct
    13501 tttcgttttt cttttcgagc tttgtttcgt aatcaaattt aatctttaaa tattccctga
    13561 gatttgccga catgggaagc ggcagattag gcagcctgct tttgtcagcg agtacagcct
    13621 tataataatc cttaacgcag ctttcgacat aagacaaata gggcttcata tcctcatcat
    13681 tggctttgtc gaattcgctt accttaaaat cgctttctct ctttgtggtg gtttcatcct
    13741 ttccagattt ggaacaggaa cagaaaccaa agagcaaaga aagcaaaagt atgctgacta
    13801 taatctgttt atgtacgctg cccataagcc actccccttt tatttattta aggaaatcgg
    13861 tattgatatt aaccttgtat ggcccactca gagagctgat atcatccgcg ctcatggtct
    13921 gacgcttgcc gccgcaggaa agcactttga caagaatctc agcggccttt tcaacagtgt
    13981 gcataagacc gaaggtttcg tcaaagtcgg ctcccgagca gaacacgccg tgatgcgccc
    14041 agataataac atcgtgctcc tttatccgct ctgcggtttt aagcgcgata tctgcgctgc
    14101 cgggaaccat ccacggcaga acgcccacgc ctcgcgggaa tacaattggg cactcggtca
    14161 tcatttccca cagttccctt gagaaaacct ttgaatcaag gggcagaacg aatgtaagcg
    14221 caatggtatt cgctggatgg gaatgcatta taaccctatg caaaccattt gtcttttcct
    14281 tttttatgct gtgatttata aggtgggtcg gaagctcgct tgtgggaacg ccaccggaag
    14341 ctaagcccca tacaattctg tatgcgctgc cgctttcgtc gatctcgatt atggcgacat
    14401 tgttctctat atccgccttg acattttgaa agtactttcc gctgcctgtt acaaggaaaa
    14461 attcattctt aaggttttct acagtgacgc cgataggctt aaactcaccg tcgaatgaaa
    14521 gctgagaaag ataaggcttt aattcctcat ctttaatgcg gtatgagagg tttccgccgt
    14581 ttctctcatg ccagcctttg ttatacgcgt catcgcacag ctttgcaaac gcctttacaa
    14641 agcctgcatc aattatgctg ctcatatttg ctccttaatt cctcaaagat ttactgcctt
    14701 ttgcttaata cattcttttc gtattcttct actgccttat accattcatt cctgacgggg
    14761 acattgttca tttcgcagaa gcggtcccaa acatcggaga agggcatggt tttaaactcc
    14821 tcattcatca tgagcatgcg ggtaaagtca cccttttcct gcaataactt catctcttta
    14881 agaggcataa gcaaagcgta aagcagagct ttttgcatat ttctcatgcc tataacccaa
    14941 gccgcgatgc gatttatgct tgcgtcaaag aagtcaagac cgattaagac tctgtctagg
    15001 gcgtggtttg cgacaatttc cttcgcgatc tcgattaact cgttatccag agttaccaca
    15061 tggtcgctgt cccatctgac tccccttgtg acatgaaggg ctattttgtc gtggaaaagc
    15121 agaagcgacg acagcttatc ggagataacc tcggtcggat ggaaatgacc gctgtcaagc
    15181 aggcaaagca catcattttt ggaggcatag cttaaataaa actcgtgtga gcctaccgta
    15241 taggactcaa cgcctatgcc gaaaagcttt gactctacgc tgtcataaag ataatctctt
    15301 gagtattttt ccgaaaaaat ctcatcaagg gattccttaa gcctttccct cggacccttt
    15361 ctgtctgcgg gagtctcctt atatccgtcg ggaatccaga tattgttaag gcaaggctgg
    15421 ccaagctctt tgccaaagta ctcgcctact ttgcgcattg ctttgcagtg ctcaatccag
    15481 aaatccctga ttttcttatc gggatgagaa agagtcaggg tgtcggacgc gtattcatgg
    15541 gcgaaaagag tggggttaat gtcaagcccc agtcccctct cctttgcgaa ctcaacccat
    15601 ttttcaaaat gaacgggctg gagcttgttg cgggggacct tttcgccgtt tgttacagca
    15661 tagatagcgt gaaggttcag cttgtgcttt ccgggaataa gggagaaggc catgtctatg
    15721 tcggacatta gctcttcggg agtccttgct ttacccgggt aattgcccgt ggcggcaata
    15781 ccgccggaaa gctcgcccgc gccctcaaag cccgagacat catcgccctg ccagcagtgc
    15841 atggagattt taactttctg caaagtctct aacgccttgt cggtgtctac gcccaaatcg
    15901 gcatatacct ttttggcgtg attatatctg tcgccagcca tattgcctcc gattatctat
    15961 tttttttaag ttctattgca gcctttgcct ttgctacaat gt
//
LOCUS       DAONVQ010000030        15830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2757_length_15830_cov_4.369762, whole genome shotgun sequence.
ACCESSION   DAONVQ010000030 DAONVQ010000000
VERSION     DAONVQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15830
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2757_length_15830_cov_4.369762"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..647
                     /gene="rdgB"
                     /locus_tag="PLD98_03240"
     CDS             54..647
                     /gene="rdgB"
                     /locus_tag="PLD98_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQA05298.1"
                     /translation="MKFLIASHNLKKRDELRRILSPLGIEVYTAEDLGIVLPEVEENG
                     STFEENAELKAVSGCEASGLPCIADDSGLVVDALEGRPGIYSARYAGEDTPYSEKIKV
                     LLKEMENVPEEHRTARFVSAICCAFPDGKKIFVRGVCEGKIGFEPKGSNGFGYDPIFY
                     YEGRSFAELSPEEKDAVSHRGKSLRGLRLRLKEQFSL"
     gene            666..986
                     /locus_tag="PLD98_03245"
     CDS             666..986
                     /locus_tag="PLD98_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HQA05299.1"
                     /translation="MTGKERAALRAKANSLESYIQIGKSGISDALIKQVDDAITAHEL
                     IKIKVHLETTPISPREAADELAKALNADVIQVIGGVIVLYRYNPELHKPKQANPPKNT
                     TAKK"
     gene            complement(1061..1369)
                     /locus_tag="PLD98_03250"
     CDS             complement(1061..1369)
                     /locus_tag="PLD98_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05300.1"
                     /translation="MRLYSVIVVPIMIYAGIMYLRAHIAKSPHLFDSARKYISLSLII
                     SLIATLGSLLIFASFAVFAILFSALGFFAAMLPVVCVSAIGALWSFIGFMLEKKLRRI
                     "
     gene            complement(1668..3914)
                     /locus_tag="PLD98_03255"
     CDS             complement(1668..3914)
                     /locus_tag="PLD98_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HQA05301.1"
                     /translation="MASKAKKGTEAPKDTPELERAHILGAGEIEVQPITETIETNYMP
                     YAMSVIMSRAIPEIDGFKPSHRKLLYTMYKMGLLSGPRTKSANIVGRTMQLNPHGDAA
                     IYEAMVRLTKGNETLLHPFIDSKGNFGKAYSRNMAYAASRYTEARLEPIAAELFRDIE
                     KDTVDFVDNYDSTMKEPTLLPVTFPNILVNPNTGIAVGMASNICSFNLREVCETTIAL
                     INNPNHDISDTLNAPDFPGGGQLIYDKDALHEIYETGKGSVKVRAKYTYDKKNNCIDI
                     YEIPPTTTCEAIIEKIVELVKAGKLKEISDVRDETDKTGLKITIDLKRGVDPDKLMAQ
                     LFKRTPLEDSFSCNFNVLIAGKPMLLGVKELLEEWLAFREEAVRRRVYFDLSKAKDRL
                     HLLKGLKKILLDIDKAIKIIRDTENEQDVVPNLMIGFGIDEVQAEYVAEIKLRHLNRE
                     YILKRLEDIEKLTKDIAEMELILSDKKRIRAIIADELKDVIKKYGKDRRTEIITAAEV
                     TDAEDEEEDIPSYPVHLFFTREGYFKKITPQSLRMSGEHKLKEGDEIIAEIESDNTAE
                     LLFFTDKAQVYKAKASDFEDTKASVLGDFVPAALGMDEGESAIYMAVTKDFAGYMVFF
                     FENGKVAKIEMSSYETKLNRKKLIKAYSDKSPLVAAAYIREDTEFLLTSTAGRNLLVN
                     TGAISPKTTKDSQGVSAMTLKKGHRLASVKQYNEGMFKNPARYRTKTLPAAGMLLAKE
                     DTGEQMTM"
     gene            complement(3959..5938)
                     /locus_tag="PLD98_03260"
     CDS             complement(3959..5938)
                     /locus_tag="PLD98_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HQA05302.1"
                     /translation="MQKKVQYGNESISQLKGADRVRKRPGVIFGSDGLEGCQHSVFEI
                     ISNSIDEARSGFGTKIIVTRFLDHSIEVQDFGRGIPVDYNTREGCYNWQLLFCELYAG
                     GKYDTNSGNYEYSLGLNGLGLCATQYASEYMEAEIFTGGFRYFLQFAKGNPVGDMIKE
                     PYSGKQTGTRIKWRPDLEVFTDINIPLEYFQETLKRQSVVNPGITFILKDEISNGKFQ
                     TYEYYYKNGIEDYVREIANGQEFSSIQTWQAERIGRDRPDKPEYKVKINVSLCFSNKV
                     KLKEYYHNSSYLEYGGAPEKAVRSALVSKIDGYLKQNGKYTKTDSKISFQDIEDCLIL
                     VISSFSTQTSYENQTKKAITNKFIQEAITDFLKHQFDVYFIENPLEAEKICDQVLINM
                     RSRVRAEATRQNLKKTLATGNDLTSRVEKFVDCRSKDVSERELFIVEGDSALGACKQA
                     RDSRFQAIIPVRGKILNCLKADYAKIFKNEIITDLIKVLGCGVEVSSKSKELSSFNLD
                     NLRWNKVIICTDADVDGFQIRTLILTMFYRLMPSLINAGKVYIAETPLYEITSGGQTW
                     FAYNEAEKADALKEIGNAKFTIQRSKGLGENDADMMWLTTMNPETRRLIRVEPDSNPA
                     QIEEVFELMLGDNLQGRKDYIAEHGHEYIDLVDVY"
     gene            6401..6628
                     /locus_tag="PLD98_03265"
     CDS             6401..6628
                     /locus_tag="PLD98_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HQA05303.1"
                     /translation="MQTLKESKIGSTVRVVKLKGDGPLKRRIMDMGITKGTRIYIRKV
                     APFGDPLEVTVRGYELSLRKSEAENIIVEQA"
     gene            6643..8793
                     /gene="feoB"
                     /locus_tag="PLD98_03270"
     CDS             6643..8793
                     /gene="feoB"
                     /locus_tag="PLD98_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HQA05304.1"
                     /translation="MNIKVALAGNPNSGKTTLFNTLTGSNQYVGNWPGVTVEKKEGRL
                     KGNKDVNIVDLPGIYSLSPYTLEEVVTRDFLINEKPDVIINLVDATNIERNLYLTTQL
                     AELGIPMVIALNMIDLLRKSGDTINIEKLSKEIGCEIVEVSALRGTGVMEVANKAIKI
                     AKSQKAMQPIHKFGAEVEFALSQISDFIKNREDIAPELIRWYTIKLFERDSKVLEKLQ
                     LSKNDLEKIEKIISDIEKKFDDDAESIITNERYEFIEKVVKIAVKKKSPGMTTSDKID
                     RIVTNRVLALPIFALVVFIIYFISVSTVGTLITDWTNNVFFGEWVMGGATALLQKIGA
                     AEWLQSLIVDGIISGVGAVLGFVPQMFLLFFFLSLLEDCGYMARIAFIMDRIFRKFGL
                     SGKSFIPLLISSGCGVPGVMATKTIENEKDRRLTTIVCTFIPCGAKIPVIGLIAASML
                     PEYAGFVAPAVYFIGIFAVIVSGIALKKTKLFAGDPSPFVMELPQYHLPAFKSVIAHM
                     WERGSSFIKKAGTIIFVSSAAIWFLTNFGIANGSFGMVEGSDSFLGIIGGAIAPIFAP
                     LGFGSWEATVSTFSGLVAKENVVATFGIFFGLGADIAESDPTLVNKVAELFPNVFAGI
                     SFLIFNMLCAPCFAAIGAIRREMASGKWTLIAVGYQTLLAYIFSLMIYQLGGLISGTL
                     TFGAGSVLAIIALLFVLYMIFRPNPYVSAKKARS"
     gene            8839..9024
                     /locus_tag="PLD98_03275"
     CDS             8839..9024
                     /locus_tag="PLD98_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HQA05305.1"
                     /translation="MLEYIINNLGTIVVSLIVLALLVLAVVRIRRQRKQGGCAGCPGC
                     SDGNMYCDGCELNQQKK"
     gene            complement(9105..10988)
                     /locus_tag="PLD98_03280"
     CDS             complement(9105..10988)
                     /locus_tag="PLD98_03280"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HQA05306.1"
                     /translation="MKVTAVIVALLTLLQNFFTMIGFFPLKQTEIDYGGIPYEEPVIV
                     EPMTIIKDGASEYRIVLSDSASVSERTAAEELQYYLREISGVTLPIVSDSTEPQGKEI
                     CLGQTSREGVFYTVDRSDLGDEGFILKVFDERLVIAGGALRGTLYGVYTFLEERLGVR
                     WFTPDLTVIPEASEVIIDKQLEDKQVPVFEYRDDYWLSAYDGNWKPKQKLNSCVNGVI
                     DEKFGGGISYAYFAHSMEWLVPAELYDEHPEYFSYRKDEGKRTLDQRCLTNPDVLAIT
                     IENARRVLQSNPNAKIISITQNDNQDYCQCANCQAMDNLYGGPSGTNIWFVNHVAEAL
                     EDEFPNVQFDTFAYQYTRSAPTGIVPRHNVIVRLCSIECCFAHPLEKCGAERGDNIIE
                     KSMDQPSSFAKDIKNWSEISENLYVWDYTTNFREYLLPFPNFHVLSPNMQFFMNHNVK
                     GVFEQGNHTGGKSGEFGELRAYIIAKLLWNPYADVEYHMMDFMRHYYGVEAAEYIKEY
                     IDFITNKNTQLSHLFIFNRSDEMFHMTASERKMVEKLWENALNSDITEQQRENIERSY
                     LSYRYYKATKLYDEFSLLSFTRMEENKKLHADMKRLGIKRLTEWHDLPDNPWFLLPPN
                     EWQ"
     gene            11207..12880
                     /locus_tag="PLD98_03285"
     CDS             11207..12880
                     /locus_tag="PLD98_03285"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HQA05307.1"
                     /translation="MSKYTIGIDYGTLSGRVLVANVETGEEVASYVLDYPHAVMSETL
                     PNGTKLGLDWALQHPQDYLDVLYKAVPEAVKLSGVSPDDIIGLGVDFTACTILPVKKD
                     GTPLCFIDEYKNNPHAYVKLWKHHAAQDKANKLNEIAEERGEEWLKNYGGKISSEWAI
                     PKVWQILDEAPEIYDAADLFIEAGDWIVWQLIGKENRSSCFAGYKCNWNKQKGFPSKD
                     FFKALDPRLENFIDEKWSRDISPTGTKAGELTAEMANKLGLKPGIAVAVAIIDAHVFV
                     PAAGVAEPGKLVAIMGTSTCHMLLSDEGTPVEGISGVVEDGILPGFFGYEAGQACVGD
                     HFQWFVENCVPKAYFDEAEKLGLNIHQYLRSKAEKQKPGEHGLVALDWWNGNRSVLID
                     GDLSGVMLGMTLSTKPEDMYRALIEATAYGTRIIVDTFKNGGITINEFYAAGGIAKKD
                     PMTMQIYADIINMPIKIVSSDQGGALGSAIFGAVAAGKANGGYDDVLTAAKTMGKVLD
                     IEYRPNPENVKIYNKLFEEYKILHDYFGRGVNDVMKRLKAIKREVLSQN"
     gene            12904..>15830
                     /locus_tag="PLD98_03290"
     CDS             12904..>15830
                     /locus_tag="PLD98_03290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQA05308.1"
                     /translation="MTPFTHLHVHTEYSLLDGAARIKPLVQRAKELGQTALAITDHGA
                     MYGVIDFYLAAKEVGIKPIIGCEVYVAERSRFDKVHGIDNVRYHLVLLCENNTGYKNL
                     IKMVSDAWINGFYTKPRVDKELLQKYNEGIIALSACLAGEIPQALLEGNYEKAKEIAL
                     WYKNVFGKDNFFLELQDHGLEEQIKINPDIIRLSRETDIPLVCTNDSHYILKEDARVQ
                     KLLICLQTDKTINEDTGLEFSTEEFYLKSGDEMAALFPEVPEALENTNNIAERCNVEF
                     EFGQTKLPHFEVPNGMDHCEYFRQLCYEGFKKNYGDNAPPEYKERLDYEIEIINKMGY
                     IDYFLIVRDFIHYAKTQGIPVGPGRGSGAGSIAAYCVGITGIDPMRHNLLFERFLNPE
                     RVSMPDFDVDFCYERRSEVIDYVVRKYGADHVAQIVTFGTLKAKAAIRDVGRVLGMPY
                     VAVDKVAKLIPNELNITIDAALKKSQDLLNLYETDPQVKELIDYARKIEGMPRHTSKH
                     AAGVVISNLPVDEYVPLARNDDSIVTQFPMTTIEQLGLLKMDFLGLRTLTVISDAEKM
                     IRRKEPNFSINNIDLEDKATFDMLSEGSTFGVFQCESAGIRSLMINMKPRNIEDIIAV
                     IALYRPGPMDFIPQYLSNRQHPDKIEYLAPQLKNILDVTCGVIVYQEQVMQIFRELAG
                     YSLGRSDLVRRAVAKKKADVLAKEKEHFLYGLKNEDGTYACPGCIKNGISEEAANKIF
                     DDMSSFAQYAFNKSHSAAYAYVSYQTAYLKCHYPREFMAAILTSVLDNTDKVAAYIGE
                     CHNLGIKVLPPSVNESLEGFTVTNDAIRFGLLAVKNLGRGFINLILEEREKNGPYKSF
                     YSFCKRVYGKEFNRRAIESLIKCGALDGLGLNRRQMLEMIEDVIASLDSEKKKNVEGQ
                     ISFFDLSQELSEASEPVPKDLEEMPERELLALEKATTGLYLSGHPMAEYKDVSKKINS
                     ART"
BASE COUNT         4307 a         3569 c         3722 g         4232 t
ORIGIN      
        1 gaaaaggtct gtttggcgtt ttttctaaaa agtaagcttt aggaggaaaa gtcttgaagt
       61 ttttaatcgc aagccataac ctgaaaaaga gagatgaact gcgcagaatt ctttctccct
      121 tgggtataga ggtttatact gccgaggatt tgggaatagt attaccggag gtagaagaga
      181 acggcagcac atttgaggag aacgccgagc tcaaggctgt cagcggctgc gaagcttccg
      241 gtttgccttg cattgccgat gattcgggtc ttgtggttga cgctttagag ggcagacctg
      301 gcatctacag cgcaaggtat gcgggcgagg atactcccta ctcagaaaaa attaaggtgc
      361 tgcttaaaga aatggaaaat gtccctgagg agcaccgcac cgcaaggttt gtaagcgcga
      421 tttgctgcgc tttccccgac ggtaaaaaga ttttcgtcag gggagtatgc gaaggcaaaa
      481 taggctttga acccaaaggc agcaacggct tcggatatga cccgattttc tattacgagg
      541 gcaggagctt tgcggagctt tctcccgaag aaaaggacgc cgtaagccac aggggcaagt
      601 cgctgagagg ccttagatta agactaaaag agcagttttc attataaaat aacggaaggt
      661 atgctatgac aggtaaagaa agagcagcat taagagcaaa agcaaactca ttggaatcat
      721 atattcaaat cggcaaatct ggcatttcag atgcgctgat taagcaggtt gatgacgcca
      781 ttaccgcaca tgagcttatt aagattaagg tgcatcttga gaccacgccg atttcaccga
      841 gagaagcggc cgatgagctg gctaaagctt taaacgccga tgttattcag gtcatcggcg
      901 gcgttatagt gctgtacaga tataatcccg agcttcataa gccgaagcag gcaaatcctc
      961 caaagaatac aacagcaaaa aaataaagcc taattcaaga taaagcctaa ttcaaggctt
     1021 tgtactaaaa gaaaaacctg cgattttata cgcaggtttt ttatattctt ctgagttttt
     1081 tctcaagcat aaaaccgata aagctccaga gcgctccgat tgccgatacg caaacaacgg
     1141 ggagcatcgc agcaaagaat cccaaagccg aaaacaaaat ggcaaaaaca gcgaaagatg
     1201 cgaagataag cagcgagccc aaggttgcaa tcagagaaat tatgaggctg agacttatat
     1261 acttgcgggc ggaatcaaaa agatgcggac ttttagctat atgagctctt aaatacataa
     1321 ttcccgcgta gatcattatg ggcacaacaa ttactgaata aagcctcata agccccgaag
     1381 ccagtaattt gtttgttgca ttgaagatat aaaacagccc ggggttaatt actgcaatgc
     1441 aaagaatgat aataccgccc aatagccacc aaataatatt aagaataagc aaggcttgtt
     1501 tttttctcat acaacgcatc cttaaatttg tttcatagaa attatgaaat aagagtatta
     1561 aatacatatt aagaagtttt taattatact gaaaagaatt aagaactatt ttgaggaatt
     1621 gtgtaaacaa aaaagcagag agctttcgct ctctgcttta gacccactta cattgtcatt
     1681 tgctcgcccg tgtcttcttt tgcgagcagc attcctgccg cagggagcgt ttttgttctg
     1741 tatctcgcgg gattcttaaa cattccttca ttatactgct taacagatgc cagcctgtgt
     1801 ccttttttca gagtcattgc ggaaacgccc tgagagtcct ttgttgtctt ggggctgatg
     1861 gctcctgtat ttacaagcag attcctgccc gcggtggagg tgagtaagaa ttcagtatcc
     1921 tccctgatat aagcggcggc aacaagcggg ctcttgtccg aatatgcctt aatgagcttc
     1981 tttctgttaa gcttggtttc ataagaggac atttcgattt tagcgacctt gccgttctca
     2041 aagaagaaga ccatatagcc cgcaaaatcc tttgtaacgg ccatatagat tgccgattcg
     2101 ccctcgtcca tacccagagc cgcgggcaca aagtcgccca gaacgcttgc ctttgtatcc
     2161 tcaaagtcgg atgccttagc cttgtaaacc tgagctttgt cggtaaagaa caaaagctca
     2221 gcggtattat cggattcaat ttcggcaatg atttcgtcgc cctctttgag cttatgctcg
     2281 ccgctcatgc gcagcgactg gggagtaatc ttcttgaaat agccttctct tgtaaagaag
     2341 aggtgtacgg ggtagctggg aatatcctct tcttcgtcct cggcgtcagt gacttcggcg
     2401 gcggtaataa tctcagtgcg cctgtctttg ccgtatttct taattacatc ttttagctca
     2461 tcggcgataa ttgctcttat tctctttttg tctgagagaa taagctccat ttcggcaata
     2521 tctttggtga gcttttcaat gtcttcaagg cgttttaaga tatactccct gttaaggtgc
     2581 ctcagcttaa tctcggctac atactctgcc tgaacctcgt caatgccgaa tccaatcatc
     2641 aaatttggaa cgacatcctg ttcgttttcg gtgtcgcgta taatcttaat tgccttgtca
     2701 atatcaagga gaattttctt taagcctttg agcaggtgca gcctgtcctt tgcctttgat
     2761 aggtcaaaat agaccctgcg cctgactgcc tcttctctaa aggcaagcca ctcttcaaga
     2821 agctccttaa cacctaaaag catcggcttg cctgctatga gcacattgaa gttgcaggag
     2881 aaactgtcct caaggggagt tcttttaaac agctgagcca ttaatttatc agggtcaacg
     2941 cctcttttaa ggtcaattgt gattttaaga cccgttttgt cggtttcatc cctgacatcg
     3001 ctgatttcct ttagcttgcc cgcctttaca agttccacaa tcttttcaat tatagcctcg
     3061 caggtggtgg ttggcgggat ttcatatatg tctatgcagt tgtttttctt atcataggtg
     3121 tatttcgctc tgacctttac agaacctttg cctgtttcgt aaatctcatg gagggcgtcc
     3181 ttgtcgtata tgagctgacc gcctccgggg aagtcgggag cattgagagt atcggaaata
     3241 tcgtgattcg ggttgttaat aagggcaatg gtggtctcgc aaacttctct taaattgaag
     3301 gagcagatat tacttgccat gccgacggcg attcccgtat ttggatttac aagtatattg
     3361 gggaaggtga cgggcaggag agtgggctcc ttcattgtgc tgtcatagtt gtccacaaag
     3421 tcaacggtgt ccttttcaat atccctgaaa agctccgcgg caatcggctc aagacgggcc
     3481 tctgtgtaac gggaagcggc gtaagccata ttcctcgaat aagccttgcc gaagttgccc
     3541 tttgaatcta taaatgggtg caaaagtgtc tcgttgccct ttgtgagcct taccattgcc
     3601 tcataaatag cggcgtcgcc gtgggggttc agctgcattg tacggcctac gatattggcg
     3661 gatttggtcc tcggaccgct taaaagcccc atcttataca ttgtatataa gagcttgcgg
     3721 tgggagggct taaaaccgtc aatttcggga atggctcttg acataataac gctcatggcg
     3781 tagggcatat agttggtttc aatggtttcg gtaattggct gaacttctat ttccccagcg
     3841 ccgagaatat gagctctctc cagttccggt gtatctttag gcgcttcggt gcccttttta
     3901 gcttttgaag ccatttaata tatcactcca aaataatctg aaactgatta aatatgattt
     3961 agtaaacatc aaccaagtct atatattcat gaccgtgctc agcgatataa tcctttctgc
     4021 cctggaggtt gtcaccgagc ataagctcga aaacctcctc gatttgggca gggttggaat
     4081 cgggctcaac tctgatgagc ctgcgggttt cggggttcat ggtggtgagc cacatcatgt
     4141 cggcgtcatt ttcgccaaga ccctttgacc tctgaatggt gaacttggcg ttgccgattt
     4201 ctttgagcgc gtcggctttt tcagcctcgt tataggcaaa ccatgtctga ccgcccgatg
     4261 taatctcgta aaggggagtt tccgctatat aaaccttgcc agcgttgata agcgagggca
     4321 tgagcctgta gaacatggtg agaatcagtg tcctaatctg gaatccgtca acatccgcat
     4381 cggtgcaaat tatgaccttg ttccatcgga gattgtcgag attgaaggag gagagttcct
     4441 tagacttgga gctgacctcc acgccgcagc cgagaacctt aataaggtcg gttataatct
     4501 cattcttgaa aatcttggcg taatcggctt ttaggcagtt taggattttg ccccttacgg
     4561 ggatgatagc ctggaatctt gagtcccttg cctgtttgca ggcgccgaga gccgagtcac
     4621 cctctacaat gaaaagctcc ctttcggaaa catccttgct acggcagtca acgaatttct
     4681 caactctgct agtaaggtcg ttgcccgtgg cgagtgtctt cttaaggttt tgccttgtgg
     4741 cttccgctct gacccttgag cgcatattaa taagaacctg atcgcagatt ttctccgctt
     4801 caaggggatt ttcaatgaaa tatacatcaa actgatgctt taagaaatca gttatagcct
     4861 cttgtataaa cttgtttgta atggcctttt tggtttggtt ttcgtatgaa gtctgagttg
     4921 agaaagatga aataacgaga attaagcagt cctcaatatc ctgaaagcta attttgctgt
     4981 cggtctttgt gtatttgccg ttctgcttta gatagccgtc aatctttgaa acaagggcgc
     5041 ttcgaacagc cttttcgggc gcgccgccgt actcaagata gctggagtta tgatagtact
     5101 cctttagttt tactttgttg gaaaagcaga gcgaaacatt tatctttacc ttgtattcag
     5161 gcttgtccgg tctgtcacgg ccaatacgct cagcctgcca agtctgaatg cttgaaaact
     5221 cctgaccgtt cgcaatctct ctgacataat cctcaatgcc gtttttgtag taatactcat
     5281 aggtctggaa cttgccattt gagatttcgt ccttaagaat aaatgttatt ccggggttta
     5341 caaccgactg gcgctttaag gtctcctgga aatattcgag gggaatatta atatcggtaa
     5401 agacctcaag gtcgggacgc cacttaattc ttgttcccgt ttgcttacca gaataaggct
     5461 ctttaatcat atcgccaacg ggattgccct ttgcgaactg aaggaagtac ctgaagccgc
     5521 ccgtgaaaat ttcagcttcc atgtactccg acgcatactg agtagcgcag agaccaagac
     5581 cgttaagtcc taaagagtat tcatagtttc cgctgtttgt gtcgtacttt ccgccggcgt
     5641 aaagctcgca aaataaaagc tgccagttat aacagccttc tctggtgtta tagtcgacgg
     5701 gtatgcctct gccgaaatcc tgaacttcaa tagagtggtc aagaaatctt gtaactataa
     5761 ttttggttcc aaagccggac cttgcttcat ctattgagtt ggagataatc tcaaaaaccg
     5821 aatgctgaca gccttcaagc ccgtcggagc cgaaaattac tcccgggcgt tttctgactc
     5881 tgtcggcgcc tttaagctga gatatgcttt cgttaccgta ctgaaccttt ttctgcattt
     5941 ctgcctccgt taaatctata taaggggtat gacagccgct aaataacaat atttagtaat
     6001 caaatcttat attatgttac actatccggt cggctttagt caagaaatcc ctgtaattat
     6061 agcgtttggg gcttgatatt ttaattttgg gtgctataat aaaatcagag ttagcagagg
     6121 gtaactcctc gaaatttccc gcatataatt taatgaatta aaaaatttga cccataaatt
     6181 ctgaaaaaaa atcatacaaa taggacaggg caaattgtca aaaaatctac ataacaagta
     6241 ttgacaatat tagttagcag tagtaaactt aatgcggcta atgagttagc ctctgctaat
     6301 caagttaaaa atctgtcaat atttttgtaa aactgtacca ttatttaaca caattacaga
     6361 tgtaaacaat ccgtgtgggg ttaatacagg aggaaacaat atgcagacct tgaaagagag
     6421 caaaataggc agtaccgtta gggtggtaaa actcaagggt gacggcccct taaaacgcag
     6481 aattatggat atgggcatta ctaagggcac ccgaatttat ataagaaaag ttgccccttt
     6541 cggtgacccg cttgaagtta ctgtacgagg ctacgagctt tctttaagaa aaagcgaggc
     6601 tgaaaacata atcgtcgaac aggcataaag caggaggaat ttatgaacat taaagtagca
     6661 cttgcgggaa acccgaacag cggtaaaacc acgcttttta acactcttac aggctctaac
     6721 caatatgtgg gaaactggcc cggagtaact gtcgagaaga aggaaggcag actaaagggc
     6781 aacaaggatg taaacatcgt tgacctgccg ggaatatact ctctgtctcc ctatactctt
     6841 gaagaggttg ttacaagaga cttccttatt aacgaaaaac ccgatgtaat tataaatctt
     6901 gtagacgcga ctaatatcga gcgtaaccta tacctgacaa ctcagcttgc cgagcttggc
     6961 atcccgatgg ttatcgctct taatatgata gatttattga gaaaatccgg cgacactatc
     7021 aatattgaaa agctctcaaa agagataggc tgcgaaattg tcgaggtttc tgcactcagg
     7081 ggaacgggcg taatggaggt tgctaacaaa gcaatcaaaa tcgcaaagtc gcaaaaggca
     7141 atgcagccca ttcataaatt cggggctgag gttgagttcg ctctcagcca gataagcgac
     7201 tttataaaga acagagaaga tattgccccg gagcttataa gatggtacac aattaagctt
     7261 tttgagcgcg acagcaaggt tcttgaaaag ctccagcttt ctaaaaatga cttagagaaa
     7321 atcgaaaaaa taatatccga tattgaaaag aaattcgatg acgacgccga aagcattatc
     7381 acgaatgaaa gatacgagtt tatcgaaaag gttgtaaaaa ttgcggtcaa gaaaaagagc
     7441 ccgggcatga caacctcgga taaaatcgac aggatagtca ctaacagagt tctggctctt
     7501 cctatatttg cccttgtggt tttcataatt tactttatat ccgtttccac agtgggaact
     7561 ctaattaccg attggaccaa caatgtattc ttcggtgaat gggttatggg cggagcaacc
     7621 gccttgctcc aaaaaatcgg cgctgccgag tggctccaaa gccttattgt agacggcatt
     7681 atctcgggcg tcggcgcggt tctcggcttt gttccgcaga tgttccttct tttcttcttc
     7741 ctttcactcc ttgaagactg cggctatatg gcaagaattg cgtttatcat ggacagaatt
     7801 tttcgcaagt tcggactttc gggaaagagt tttatcccac tgcttataag ctcgggatgc
     7861 ggagttcccg gtgtaatggc taccaaaaca attgagaacg aaaaagaccg caggcttacc
     7921 accatcgttt gcacctttat tccctgcggc gcaaagattc cagtcatcgg acttatagcg
     7981 gcctcaatgc tcccggaata cgcaggtttt gttgctcccg cggtttactt tatcggcatt
     8041 ttcgctgtta tagtatcggg catagccctc aagaaaacta agctctttgc tggtgaccct
     8101 tcgccctttg taatggagct tccccagtac catttgcccg catttaagag cgttatcgcc
     8161 cacatgtggg aaagaggcag ctcgtttata aagaaggcgg gaacgataat cttcgtatca
     8221 agtgcggcaa tctggttcct tacaaacttt ggaatagcga acggctcgtt cggaatggtt
     8281 gaaggcagcg acagcttttt aggtattatc ggcggcgcaa ttgctcccat attcgcgccc
     8341 cttggtttcg gctcatggga ggcaacggtt tcaacattct cgggtctcgt cgcaaaagaa
     8401 aatgttgtcg ccacattcgg aattttcttc ggtctcggag ccgacattgc cgaaagcgac
     8461 ccgacacttg taaacaaggt tgcggagctt ttcccgaatg ttttcgcagg catttcattc
     8521 cttatcttca atatgctttg cgctccctgc tttgcggcta tcggagctat cagaagggaa
     8581 atggcttcag gaaaatggac attgattgcg gtaggctatc agaccctttt ggcatacatt
     8641 ttctcgctta tgatttatca gcttggcgga cttatttccg gaacacttac tttcggcgcg
     8701 ggctcggtat tggcaatcat cgcattactg ttcgtgctct acatgatatt cagacccaat
     8761 ccgtatgtta gcgcaaaaaa ggcaaggagc taagctccgc gggctgagaa ggctttgcct
     8821 tcagaatgga gtgatactat gctcgaatat attatcaata acctcggtac aatagttgtt
     8881 tcattaattg ttcttgcgct gcttgtcctt gcggtggtta gaatccgccg tcagcgcaag
     8941 cagggcggct gcgcaggctg cccgggctgc agtgatggca atatgtactg cgacggctgt
     9001 gagttaaacc agcagaaaaa ataacaaatg cccgctctgc gggctttttg ctgagagtta
     9061 gaaacagaaa aaagcctccc ttttcgggag gcttaaactt ttatttattg ccactcattg
     9121 ggcgggagga ggaaccaggg attgtcgggc aggtcatgcc actctgtaag acgcttgata
     9181 ccaagacgct tcatatcagc atggagcttt ttgttttctt ccattcttgt aaagctcagc
     9241 aacgagaatt catcataaag ctttgtagct ttgtaatacc tgtaggagag gtaggagcgc
     9301 tcgatattct ctctctgctg ctcggtaata tcggagttaa gagcattctc ccagagcttt
     9361 tcaaccattt tgcgctcgga agctgtcata tggaacatct catccgacct gttgaagatg
     9421 aagaggtgtg aaagctgagt attcttattg gtgatgaagt caatgtactc tttaatgtac
     9481 tcagcggctt ccacgccata gtaatgacgc atgaagtcca tcatatgata ctcaacatcg
     9541 gcataggggt tccagaggag cttagcgatt atgtaagctc tcagctcgcc gaattcgccg
     9601 ctcttgccgc ctgtatggtt gccctgctca aatacgccct taacattgtg gttcatgaag
     9661 aactgcatgt tgggggagag aacatggaag ttggggaagg ggaggagata ctctctgaag
     9721 ttggttgtgt agtcccaaac atagaggttc tcgctgattt cgctccagtt cttgatatcc
     9781 tttgcgaagg aggagggctg atccattgac ttctctataa tattgtcgcc gcgctcagcg
     9841 ccgcactttt caaggggatg agcgaagcag cactcaatgg agcagagacg gacaataaca
     9901 ttatgtctcg gaacgatgcc agtgggagca gaacgggtgt actgatatgc gaatgtatcg
     9961 aactgaacat tcgggaactc gtcctcaagt gcttccgcta cgtgatttac aaaccagata
    10021 ttagtgccgg aaggaccgcc gtacagattg tccatagcct ggcagtttgc gcactggcaa
    10081 taatcctgat tgtcgttctg agtgatggag attatctttg cgttgggatt agactgcaaa
    10141 actctccttg cgttctctat tgtaatagcg agaacatcgg gatttgtaag gcagcgctgg
    10201 tcaagagttc tcttgccttc atcctttctg taggagaagt actcgggatg ctcatcataa
    10261 agctctgcgg gcacaagcca ctccatggaa tgggcaaagt aggcataaga gatgccgccg
    10321 ccaaactttt catcaataac gccgtttacg caggaattga gcttctgctt cggcttccag
    10381 ttgccgtcgt aagcggagag ccaatagtca tccctgtact caaatacggg gacctgcttg
    10441 tcctcaagct gcttgtcgat aattacttcg ctagcctcgg ggataacggt caaatcgggg
    10501 gtgaaccatc tgacgcctaa tctttcctca aggaaggtgt agactccgta aagcgttccc
    10561 cttaaggcgc cgcctgcgat tacgagcctt tcgtcaaaga ccttaagtat aaagccttcg
    10621 tcgccaaggt cgctgcggtc aacagtgtag aaaacgccct ctcttgaggt ttgtccaagg
    10681 caaatttcct taccctgagg ctccgttgag tcggaaacga taggcagtgt tacgccactg
    10741 atctccctta aataatactg gagctcttcg gctgccgttc tttccgaaac ggatgcgctg
    10801 tcggacagaa caattcggta ctcgctggca ccgtctttaa tgattgtcat aggttccaca
    10861 ataactggct cctcgtaagg aattccgccg tagtcgattt cggtttgctt cagcgggaaa
    10921 aaaccgatca tggtaaagaa gttttgcaaa agcgtaagta gtgcaactat tactgcagtt
    10981 actttcattc tatcacctcg gtgaaatatt accataaccg tgtttcgaaa acaagagata
    11041 taagtcaata tgcacaaata tttttccatt gttgaaattt tttatattaa gtagctaaca
    11101 ggcattgatt aattatcaat aatgcaactt gaaaaagacg cacgtatgcc ttattattat
    11161 ttatagtaaa tattatgatg attaaatggc tatcggagga attggaatgt ctaaatacac
    11221 aatcggcatt gactacggta ccctttcggg cagagttctt gttgctaatg ttgagacagg
    11281 cgaagaggtt gcgtcctatg ttttggatta cccccacgcg gttatgtccg aaacactgcc
    11341 taacggcaca aagctcggtc ttgactgggc tttgcagcac ccgcaggact atctggatgt
    11401 tctttacaaa gcagtacccg aggcagtaaa gctctcaggc gtttcacccg atgacattat
    11461 cggtcttggc gttgatttta cagcctgcac tatacttccc gtaaaaaaag acggcacgcc
    11521 cttgtgcttt attgatgaat acaaaaacaa cccccacgct tatgttaagc tctggaagca
    11581 ccacgccgct caggataagg caaacaagct taacgaaatc gccgaggaaa gaggcgagga
    11641 gtggcttaaa aactacggtg gcaagatttc ttccgaatgg gctatcccca aggtatggca
    11701 gattcttgac gaggctccgg agatttacga tgccgcggat ttattcattg aagcaggcga
    11761 ctggattgtg tggcagctca taggcaaaga gaacagaagc tcctgctttg ccggttataa
    11821 gtgcaattgg aacaagcaaa agggattccc gtcaaaggat ttcttcaagg ctcttgaccc
    11881 cagacttgag aacttcatag atgaaaaatg gtccagggat atttctccca caggcacaaa
    11941 agcaggcgag ctgactgccg aaatggcaaa taagctcggt cttaagcccg gcattgcggt
    12001 tgctgtcgct attattgacg cacatgtatt tgtacccgcc gcaggcgttg ccgagcctgg
    12061 caaactggta gccatcatgg gcacatccac ctgccacatg ctcctttccg atgagggcac
    12121 acccgtagag ggaatttctg gcgtagttga ggacggtatc ctccccggat tcttcggcta
    12181 tgaggcaggt caggcatgcg tgggcgacca tttccagtgg ttcgttgaaa actgcgttcc
    12241 caaggcatat tttgacgaag ctgaaaaact gggtctcaac attcaccaat acctccgcag
    12301 caaagccgaa aagcaaaagc cgggcgagca cggtcttgtc gctcttgact ggtggaacgg
    12361 caaccgctcc gttctcatcg acggcgacct cagcggcgta atgctcggca tgaccctttc
    12421 cacaaagcca gaggatatgt atagggcttt aattgaggca acagcctacg gcacaaggat
    12481 tattgttgac accttcaaaa acggcggcat tacaataaat gagttctacg ccgcgggcgg
    12541 cattgccaaa aaagacccga tgacaatgca gatttatgca gatataataa atatgcccat
    12601 taagatagtc agcagcgacc agggcggcgc gttgggctcc gcgattttcg gcgctgttgc
    12661 agcaggcaag gcaaacggcg gctatgacga tgttctcacc gccgcaaaga ctatgggcaa
    12721 ggttcttgat attgaataca gacccaatcc tgaaaatgta aagatttata acaagctgtt
    12781 cgaggagtac aagattctcc acgattactt cggcagaggc gtaaatgatg taatgaagcg
    12841 ccttaaagcc ataaagagag aagttctttc acaaaactaa gctaatctcg gaggaaggac
    12901 aagttgacac cctttaccca cttgcatgtt cataccgaat acagcctgct ggacggagct
    12961 gcccgcataa agccgctcgt tcagcgggct aaagagcttg gtcaaacagc ccttgcgata
    13021 accgaccacg gagcgatgta cggcgttatt gacttttatc tcgccgcaaa agaagtcggc
    13081 ataaagccca ttatcggatg cgaggtatat gtggctgaaa gaagcaggtt tgacaaggtt
    13141 cacggtatcg acaatgtaag ataccatctt gtccttcttt gcgaaaacaa tacgggctac
    13201 aaaaacctta tcaaaatggt gtccgacgcg tggataaacg gtttctacac aaagcccaga
    13261 gtagataagg agcttttgca aaagtacaat gagggcatta tagccctttc cgcctgcctt
    13321 gcgggcgaga ttcctcaggc tctgcttgag ggaaattatg aaaaggctaa ggaaatcgcc
    13381 ctctggtaca aaaatgtatt cggcaaggat aatttcttcc ttgagcttca ggaccacggt
    13441 cttgaggagc aaataaagat taatcccgat attataaggc tttccagaga aaccgatatc
    13501 ccccttgtct gcaccaacga cagtcactat atattaaagg aagacgcaag ggtacaaaag
    13561 ctcttaatct gccttcagac agacaagaca attaatgagg atacgggact tgagttctcg
    13621 accgaagagt tctatctaaa atcgggcgat gagatggcag ctttgttccc cgaagtcccc
    13681 gaagctcttg aaaataccaa taacatagcc gaacgctgca atgttgagtt tgagttcggt
    13741 caaaccaagc tgcctcattt tgaggttccg aacggaatgg accactgcga atacttcagg
    13801 cagctctgct atgaaggctt taagaagaat tacggggata acgctccgcc cgaatacaaa
    13861 gaacgccttg actatgagat agaaataatc aacaagatgg gctatatcga ctacttcctt
    13921 atagtccgtg actttataca ttacgcaaag actcagggta tccccgtggg tcccggacga
    13981 ggttcaggcg cgggcagtat tgccgcttac tgcgtgggca ttacgggcat tgacccgatg
    14041 cgccataacc tgctgtttga gcgcttcctt aaccctgagc gtgtcagcat gcccgacttc
    14101 gatgttgact tctgctatga acgcaggtct gaggttattg actatgtcgt cagaaaatac
    14161 ggcgcggacc atgttgccca gatagtaacc ttcggtactc ttaaagcaaa agccgctatt
    14221 cgagatgtag gcagagttct gggcatgccc tatgttgcgg ttgacaaggt agcaaagctc
    14281 atccccaatg agctgaatat caccattgac gcggctctca aaaaatctca ggatttattg
    14341 aacctgtacg aaaccgaccc tcaggtaaaa gagctaatcg actacgcaag aaaaattgag
    14401 ggtatgccaa ggcacacctc aaagcacgca gcgggtgttg ttatcagtaa ccttcccgtt
    14461 gacgaatatg tgcctcttgc gagaaacgac gattcaatag tcacccagtt cccgatgacc
    14521 accattgagc aattgggact tttgaagatg gacttcctgg gactcagaac cctgacggtt
    14581 atcagcgacg ccgaaaaaat gataaggcgc aaggagccga acttcagcat taacaatatt
    14641 gaccttgaag acaaggcaac ctttgatatg ctttccgaag gcagcacctt cggcgtattc
    14701 cagtgcgagt ccgcaggcat aaggtccctt atgataaaca tgaagccgag gaatatagaa
    14761 gatatcatag ccgtaatcgc tctgtatcgc cccggcccga tggactttat ccctcagtac
    14821 ctcagcaaca gacagcatcc cgacaaaatt gagtatctcg ctccgcagct taaaaatatt
    14881 ctcgatgtta cctgcggcgt tatagtttac caggagcagg ttatgcagat attccgcgag
    14941 cttgcgggat attccctcgg tcgctccgac cttgtgcgca gagccgtcgc aaagaaaaag
    15001 gcggatgttc tcgcaaaaga gaaggagcac ttcctttacg gacttaaaaa cgaggacgga
    15061 acctatgcct gccccggctg cataaagaac ggcatttcgg aagaggctgc taacaaaatc
    15121 ttcgacgata tgtcgtcctt tgctcagtac gcctttaaca aatcccattc ggcggcctat
    15181 gcctatgttt cttaccagac cgcatacctt aaatgccact atcccagaga gtttatggcc
    15241 gctatcctta ccagcgtcct cgacaatacg gataaggtag cggcatatat aggcgagtgt
    15301 cataaccttg gcatcaaggt acttccgccc agcgtaaatg aaagccttga gggctttacc
    15361 gttacaaacg acgcaattcg attcggactt ttggcggtaa agaatctcgg cagaggcttt
    15421 attaatctta tacttgaaga gcgtgagaag aacggaccgt ataaatcctt ctactccttc
    15481 tgcaagaggg tttacggcaa ggagttcaac cgtagggcaa ttgaaagcct tataaaatgc
    15541 ggcgcccttg acggtctggg acttaaccgc aggcagatgc tcgaaatgat tgaggatgta
    15601 attgcctcac tggacagcga gaaaaagaag aatgtagagg gtcaaatttc cttctttgat
    15661 ttaagccaag agctgtcgga ggcatccgag cccgttccga aggacctgga ggaaatgccc
    15721 gaaagagagc ttctggctct tgaaaaggca acaaccggac tttatctttc aggccacccg
    15781 atggctgaat ataaggatgt ctcaaagaag attaactctg cccgcaccat
//
LOCUS       DAONVQ010000031        15820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2761_length_15820_cov_4.658738, whole genome shotgun sequence.
ACCESSION   DAONVQ010000031 DAONVQ010000000
VERSION     DAONVQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15820
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2761_length_15820_cov_4.658738"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /gene="der"
                     /locus_tag="PLD98_03295"
     CDS             complement(<1..1145)
                     /gene="der"
                     /locus_tag="PLD98_03295"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQA05309.1"
                     /translation="MSRAVVAIVGRPNVGKSSLFNKLCGKRLAIVDDTPGVTRDRIYG
                     DCEWLGRKFMLVDTGGIEPYSSDIILSSMREQAQIAIDSADAIIFVTDIKTGPVATDS
                     EVAGMLLKSGKPVILCVNKVDNVGDVPPEFYEFYNLGIGDPIPVSAIHGHGTGDLLDE
                     VIKHLPDEADDDEDDEVTKVAVIGKPNVGKSSLINAICGENRVIVSDIAGTTRDATDT
                     PVTNKHGKFVMIDTAGIRRKSRIDERLEKFSVLRAVMAVERADVCVIIIDATEGFTEQ
                     DSKIAGIAHEQGKACVIAVNKWDLVEKDSKTMDEYRKKLMNDFSFMSYAPIIFISAKT
                     GQRIDRLFSLIKYVHNQNSIRISTGKLNDVLAEAQAKVQPPTDKGKRL"
     gene            complement(1163..2470)
                     /locus_tag="PLD98_03300"
     CDS             complement(1163..2470)
                     /locus_tag="PLD98_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HQA05310.1"
                     /translation="MAVEIKDVVKHSPAYKKGLKKGDILLSINGHQINDVLDFQFYSK
                     NKNPVIGYKTAEGRLFLTRVYKSDDEDLGLLFETYLMDKHRRCENKCIFCFIDQLPKG
                     MRESLYFKDDDSRLSFLFGNYITLTNLTEHEVERIIEMRISPVNISVHTMNPELRVKM
                     MKNPRSGKVLQYIKRFSDAGIKINAQLVLCPEVNDGDELVFSLNELAKYYPQVQSISA
                     VPVGLTKFREKLYPLRAFTKEEAAAVIDTVEEFNDRLISEGKERLAFAADEFYIKAER
                     EIPKAEYYGEFHQLENGVGMWALFKSEFLEALENLETDSLPSPKKVSIATGVAAYPLI
                     RELCDKAESRIKGLKADVFCVLNDFFGHTITVSGLVTGQDIIKQLKGKVTGTLLLPAN
                     MFKSKDELIFLDDVTKEELEKELGAEIKVIDNNGFDFLDALIN"
     gene            complement(2550..2732)
                     /gene="rpmF"
                     /locus_tag="PLD98_03305"
     CDS             complement(2550..2732)
                     /gene="rpmF"
                     /locus_tag="PLD98_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQA05311.1"
                     /translation="MAVPKRRVSKARRDKRRANTWTLAAPTLEKCPKCGELKRSHRLC
                     SACGYYNGREVVKVED"
     gene            complement(2762..3274)
                     /locus_tag="PLD98_03310"
     CDS             complement(2762..3274)
                     /locus_tag="PLD98_03310"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQA05312.1"
                     /translation="MTVDLTSVFNNDGFSLPFDYEFSMSDYFSADEENPFSLIHAKGK
                     IFNRTGIVYLSAQSEFTLTTACDRCASDIVRSMTVPSEHILVIGNEDEAENEPDYDDN
                     RLYIPSYQLNLDDLLAEDIWLSMPVTILCSDDCKGICPSCGTNLNEETCSGKKAVDPR
                     LEALAKLLEQ"
     gene            complement(3408..4757)
                     /locus_tag="PLD98_03315"
     CDS             complement(3408..4757)
                     /locus_tag="PLD98_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HQA05313.1"
                     /translation="MRDLLRTAANVRPNGKQLNMLRNNNFYAFVHFGVNTFTDMEWGT
                     GKESPEIFNPAKLDCDQWVEAVKSAGMTGIVLTAKHHDGFCLWPSKYTEHSVRNSPFR
                     NGKGDVVKECADACAKGGIKFGFYLSPWDRNAPTYGTDAYNDYFKAQLTELLTQYGEI
                     FYVWFDGACGEGPNGKKQVYDFDGYIELINKYQPNAAIFSDFGPDIRWCGNEQGSSRY
                     AEWAVVPYELCHRNKEVHWIDSPIQGDLSYMYNCDQNIGSLSNIMYSKGLVFCPSEVD
                     MSIRKGWFYHKEEEPHFLDKLFDTYIRSVGGNCTFNLNIPPTPDGRFDERDVKRLKEL
                     GEKIRNTFSNNLAKNAKIQRVDKNADKSQCEYIIKLPAKQKISYVVIKEEIEKGQRIE
                     GFQIIDGSFANNKISIYEGTTVGNMKICPVKTQTDTLILRIISARSEALIKSIELYA"
     gene            4956..5053
                     /gene="ffs"
                     /locus_tag="PLD98_03320"
     ncRNA           4956..5053
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLD98_03320"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5152..6969
                     /gene="dnaX"
                     /locus_tag="PLD98_03325"
     CDS             5152..6969
                     /gene="dnaX"
                     /locus_tag="PLD98_03325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQA05314.1"
                     /translation="MYKVLYRKWRPSSFSDVVGQEHITVTLANEVKENRLAHAYLFTG
                     SRGTGKTTCAKILSKAVNCLNPKDGNPCNECEICVGIDKGTILDIAEIDAASNRGIDD
                     IRALREEVSFTPAKAKYRVYIIDEVHMLTIEAFNALLKTLEEPPSHVIFILATTEVHK
                     LPSTIISRCQRFDFRRISPVSIAERLEFITESEGAKITRDAALLIARIADGGLRDAIS
                     LLDRCISVSSDITADVVAKAAGIAGRDYLFKMADAICRKDVDSILSILDELHNSSLDS
                     DRLVTELVAHFRDIMIVKTVENPLDLIVCTEDELKTLKSLANSLTDEAIFRIINILTE
                     TAEKLRRSANRRALAELALIKICSEKDEKDLDSLLARIAALEKELASLKQNGFVSAPH
                     TEPAYKAENKLIQKKAEDAHQTAAQTVNDDAFGFEEEDSPEPSPFELDNEPSFFDESF
                     KNEIDTPPFDADIAPVPEEESIPFDEGGQDLPFGEGSLIDLEPDISEIPQNDDFAYAE
                     TDAQPFAGDSAKTAEKNETSDIVLTPEMWNNITYEIGRQCPPLTGILGSISGYMENGV
                     LVIKIGNALFCDLCSTKFSEQIHKAVEKAAGKAIRVKFI"
     gene            6998..7339
                     /locus_tag="PLD98_03330"
     CDS             6998..7339
                     /locus_tag="PLD98_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQA05315.1"
                     /translation="MKARIPNAAGANNMMQRIQKMQEEMAAAQKAVEESEFTASAGGG
                     LVEVTVNGKHEVTSVKINPEAMDPEEPEMLEDFLILAVNEAINKASDAIQQAMGQITG
                     GLNIPGMPSLF"
     gene            7342..7938
                     /gene="recR"
                     /locus_tag="PLD98_03335"
     CDS             7342..7938
                     /gene="recR"
                     /locus_tag="PLD98_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQA05316.1"
                     /translation="MSYNALPLQRLIEQFEKMPGIGKKTAQRLAFYILSIPKEEALEV
                     ANAIYDACEKIKRCSLCMNLTEEELCPICHSDERDKSVICVVEDAQDLLAIEKTGEYK
                     GVYHVLNGAISPLDGIGPEQLSIKELLKRLESGVSEVILATNSDIEGEATAMYISKLI
                     KPFGIKVTRLAYGLPVGSDLQYADELTLSRALKGRRTF"
     gene            7985..9592
                     /locus_tag="PLD98_03340"
     CDS             7985..9592
                     /locus_tag="PLD98_03340"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HQA05317.1"
                     /translation="MQSVYVITYDIGTTGVKTCVFEIGSKITLKGFASEGYGLYVLPN
                     GGAEQDPDEWWTAMCNTTKKALSEAVVSGDDISGISFCSQMQGLVLVDKDGKALRRSM
                     SYMDQRAEAELKEGIAHGLQIAGANVFKLLKSLKITGAVAASVKDPVYKYKWVEKNEP
                     EIFNKVYKWLDVKEYLICRMTGEFVMTHDSAFATLLYDIRKGKRCFSEAMCKMHGVNI
                     DHLPEIKNSTDKVGTLKADAARELGLKEGIAVFGGGGDASLIGVGAGSVNLGDTHIYS
                     GTSGWVSTVVDKSIVDANAMIAAIVGAQEDKFNYFAELETAGKCLEWVKDHLALDEIG
                     IYLEKVSVVDSYESVFVSLYDYMSSVIDKVPAGSGGVIFTPWLHGNRCPFEDPNARGM
                     FFNMSLETGKSELIRAVVEGVCMHLRWFLETQEKKVKTSEVIRFVGGGALSDVTCQIL
                     ADCTGRKIETIENPQNAGAVGAAVLIAVGLGLINSISDARSLIPATKSFTPDSTVKSV
                     YDKHFEVFKRLYADNKKNFADLNSKTN"
     gene            9614..10513
                     /locus_tag="PLD98_03345"
     CDS             9614..10513
                     /locus_tag="PLD98_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05318.1"
                     /translation="MIDLNTTAKIIHEGILETLKSLSFEPVYEKGADRKNPPLKKRDE
                     AHLMQYEGEKGKLRILFSGSKVFLLVAAPDAESEDDSDYKKVLTGFVDYDEYTEKDIR
                     SLANDIIDEVENNFGVKSFRSQNVKMPTPVSRTQAKSGVMSYNPLTLANRLLSMYPEL
                     KEPYKQNIQDYGDFLPEDFFLNHANKYIHETIEQNDPQKMKKLFGILNEIFEDGTNET
                     QGVIAVTILGAKYNDPDFMQKIVEYMEGGLLEAVVEVNKYLEKNRGARLRLEKPPIYK
                     PKRDKKGGLMDLLLGGQPQQSGR"
     gene            10574..11218
                     /locus_tag="PLD98_03350"
     CDS             10574..11218
                     /locus_tag="PLD98_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05319.1"
                     /translation="MRKKGLVNVLGIGINACTELSVPSLHAFLKAEGFPDIEIGIDRE
                     GTDFPGKNTYQHRLAAMEVKHKTNDECLDAVKLYRKVLSQADEKVDIAEIGFETILAG
                     LLKSNPDEFSPLSGIELIKQKVNKLWLMAGKWDDLSTGYEYNFSANQRARVAASYVCD
                     KWPTEITYLGFEVGEKVITGGSLLDGDILKDVLTAYGHPSGRSSWDPCWFCLPA"
     gene            11185..11376
                     /locus_tag="PLD98_03355"
     CDS             11185..11376
                     /locus_tag="PLD98_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05320.1"
                     /translation="MGPMLVLLACINDEEKAGYYIKRGRASLDIATGYNHFVFDANGP
                     HRFVIKKFPDSFYADMIKN"
     gene            complement(11448..11861)
                     /locus_tag="PLD98_03360"
     CDS             complement(11448..11861)
                     /locus_tag="PLD98_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05321.1"
                     /translation="MIKTRLSEDKEDTGPDINDLDAWDKKWVSIGRLSTANLAPFNNK
                     VGLYKHVIGGQVMYIGRATEWNNGGLRKKLGDYRSDNDGVRTHTSDRKIRKHLEDIVT
                     FVLVLGEDEEAAEKAKYLEKQFIGYYLPEWNYYFR"
     gene            complement(12071..13540)
                     /locus_tag="PLD98_03365"
     CDS             complement(12071..13540)
                     /locus_tag="PLD98_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05322.1"
                     /translation="MKKKLMAIVLCSALIFQAVSLVAFGAGTKNYIITDPYAEVDWDS
                     WGAYKFQPHSHTTASDGFLKIDEYVQKHYDLNYDIVALTDHGTLNKGWNKMPDTVPLI
                     RLVKRERTKMEPIVPLSDEVYYSYINGTAPSPTRTKTNGMLDVPLGIELNMATPIADC
                     HLTGYFSTYGQGLAGVFGDYETPARGVKNAGGISMLAHVGEYVYINKDTEKHVGKKID
                     EYYVNKFARIFLDNQGSCVGMGINSATDAHTRCDRILYDQILQKTIPNGVVPWGFSFA
                     DSHNERSINDAYTMVMMPELSLDGLRYAMVNGHCFAVSHYSNGVELNGMEEIPGFDEQ
                     RVYDEELYLLDNTPMVTRITVDQENDTITVEGTNFNQITWVSNGNVILREGGITDGKA
                     TLDLHSDNLLDEPYLYVRFYITGENGICYSQPFVLNVEGEEFEPVEVPVTQDISTALR
                     ALINLVDLVLFRFNPLIWLFKKVALGYSVFEGDRLNNPY"
     gene            complement(13654..14706)
                     /gene="mtnA"
                     /locus_tag="PLD98_03370"
     CDS             complement(13654..14706)
                     /gene="mtnA"
                     /locus_tag="PLD98_03370"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HQA05323.1"
                     /translation="MIKDLNGATLAYDTENNLLVIVDQTLLPREYRLLYLDELEDIFE
                     AIRSLRVRGAPAIGAAAAVGYSVLVNKIKTSDKTEFKSQADFIENRLRESRPTAVNLF
                     WALDRMEKALENALRDGIDSAKEAVKNEALFIIDEDIEMCRKIGEHGAALLKDGDTVL
                     THCNAGSLATVKYGTALSPVYVAAEQGKKIKVFADETRPLLQGARLTAFELTNAGIDT
                     TVICDNMAAKVMSEGKVNAIFVGADRIAANGDTANKIGTLSLAVNARFFGVPFYVCAP
                     YSTFDKDTPDGGGIVIEERADSEIRSLHYKESMLPDKAKVYNPAFDVTPAELITAFIT
                     PDGIIAPKDISKLFAK"
     gene            complement(14703..15515)
                     /locus_tag="PLD98_03375"
     CDS             complement(14703..15515)
                     /locus_tag="PLD98_03375"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQA05324.1"
                     /translation="MLELLKAAKTYIEGKVKTNADTAIILGSGLNHIAENLEDAVFLD
                     YRDIPAFPVSTAPGHKGRLAFGKLSGKDVICMQGRLHYYEGHCKEDIVFPIRLFKELG
                     VKTLIVTNAAGGINENFDVGDIMLITDHINLTGTNPLIGKNLDCFGTRFPDMTYAYAP
                     RLREKAKEAAEQLGIKLREGVYIGVTGPSFETPAEIRAFRVMGADAVGMSTVFEVIAA
                     VHAGIEVLGFSLITNKAAGISGKCLSGEEVNEIGRRSGEKLGKLIETTVGEL"
     gene            complement(15526..>15820)
                     /locus_tag="PLD98_03380"
     CDS             complement(15526..>15820)
                     /locus_tag="PLD98_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQA05325.1"
                     /translation="KAATVNGAKAQNRPDTGVIKEGCRADLRMIDTEWPNMRPATDMA
                     SNLVYALSGGENLMTMVDGRILYYNNEYYSFDIEKVKHEAERSVKRIISELDN"
BASE COUNT         4213 a         3775 c         3585 g         4247 t
ORIGIN      
        1 agcctcttac ccttgtcggt gggcggctga acctttgcct gcgcctccgc gagaacatcg
       61 ttcagcttac ccgttgatat tcttattgaa ttctgattgt gaacatactt gataagcgag
      121 aaaagcctgt cgattctctg acccgttttt gcggaaataa agattatcgg agcgtatgac
      181 atgaacgaaa aatcgttcat cagctttttg cggtactcgt ccatggtttt agagtccttt
      241 tcaacaaggt cccacttgtt taccgcaata acgcaagcct ttccctgctc atgggcaatt
      301 cccgcgattt ttgaatcctg ctccgtaaag ccttccgtag cgtcaatgat gatgacgcag
      361 acatcggccc tttcaaccgc catgacggct ctgagaacgc tgaatttctc cagtctttcg
      421 tcaatccttg actttctgcg tatgcccgca gtgtcaatca taacgaattt gccgtgcttg
      481 tttgttacgg gagtatccgt agcgtctctt gtggtgccgg ctatgtcaga tacaataacc
      541 ctgttttcgc cgcagattgc gtttataagc gaggacttgc ccacatttgg tttgccgata
      601 accgcaacct ttgtaacctc gtcgtcctca tcgtcatcgg cttcatcggg caggtgcttt
      661 ataacttcgt caagcaaatc gcccgtgccg tgtccgtgga tggcggaaac gggaatgggg
      721 tcgccgatgc cgagattgta aaactcataa aactcgggcg gaacatcgcc cacattatcc
      781 accttgttta cgcagagtat aacaggcttg ccgcttttta aaagcatgcc ggcgacctca
      841 ctgtcggtcg caacggggcc tgttttaatg tcggtgacaa aaataatcgc gtcggcgctg
      901 tcaattgcga tttgcgcctg ctcacgcatg cttgaaagaa ttatatccga tgaatatggc
      961 tcaatgccgc cggtgtcaac aagcataaac ttgcggccaa gccattcgca gtctccgtaa
     1021 atcctgtctc ttgtcactcc gggggtgtcg tcaacaattg caagacgctt tccgcacagc
     1081 ttgttaaaaa gcgacgactt gccgacattc gggcgtccca caatggcaac aactgctctt
     1141 gacatatttc caccgcctct cgtcaattga ttaatgcgtc cagaaaatca aagccgttat
     1201 tgtcaatgac ctttatttcc gctccaagct ctttttccag ttcttccttt gtcacatcgt
     1261 caaggaaaat gagctcatcc tttgatttaa acatattggc gggcaaaagc aaagtgcccg
     1321 taacctttcc tttaagctgc tttattatat cctgccccgt gacaagtccc gaaacagtta
     1381 tagtgtgacc gaaaaagtca ttcagaacac agaatacatc tgctttaagc ccttttattc
     1441 tgctttccgc tttgtcgcac agctccctta taagaggata tgccgcgacc cccgtggcaa
     1501 tcgaaacctt tttgggggag gggagggagt ctgtttcaag gttttcaaga gcttctaaaa
     1561 actccgattt aaacagcgcc cacattccca cgccgttttc aagctgatgg aattcaccgt
     1621 agtactcagc cttgggaatt tccctctcag ccttgatata aaactcgtca gccgcaaatg
     1681 cgagcctttc cttgccctct gagataagcc tatcgttaaa ctcctcaaca gtgtcaatga
     1741 cagccgctgc ttcttccttt gtaaaggctc ttaaaggata gagcttttct ctgaactttg
     1801 taagccctac gggaaccgca gatatgctct gcacctgcgg gtaatacttt gcaagctcat
     1861 tgagcgagaa aaccagctcg tcgccgtcgt ttacctccgg gcaaagcaca agctgagcgt
     1921 taatcttaat cccagcgtcg gaaaaacgct ttatgtattg gagcactttg cccgaacggg
     1981 ggtttttcat catcttgacc ctcagctcgg gattcattgt atgcacggaa atatttacgg
     2041 ggcttatacg catttcaata atgcgctcta cctcgtgctc tgtaagatta gtcagggtaa
     2101 tgtagtttcc gaaaagaaat gaaagccttg aatcgtcgtc tttaaaataa aggctctccc
     2161 tcataccctt aggcagctgg tcgataaagc agaatatgca cttgttctcg cagcgcctgt
     2221 gcttatccat taaatatgtt tcaaataata gacctaaatc ctcgtcgtcg ctcttgtaaa
     2281 cacgggtcaa aaacaaacga ccctcagcgg ttttgtagcc gatgacgggg tttttgttct
     2341 ttgaataaaa ctgaaagtcc agaacatcgt ttatttgatg accgttaatg ctcaaaagaa
     2401 tgtcgccctt tttaagcccc tttttgtacg caggggagtg cttaacgaca tctttgattt
     2461 ctacagccat acgatcaccg acttttaaac aatactaaaa aggcggggaa ggcaaacctt
     2521 ctccacctct tatacgctaa gacaaagaat tagtcctcaa cttttacaac ttctctgccg
     2581 ttgtaataac cgcaggcgga gcaaagtctg tgagaccttt taagctcgcc gcacttcggg
     2641 catttttcga gtgtaggagc agccaaagtc caggtatttg cgcgtctttt atcccttctt
     2701 gccttagaaa ctctgcgttt aggaactgcc atatcttaaa cctcccttac tgtgctaagt
     2761 ttcattgctc taaaagcttg gccaaagcct caaggcgtgg atcaacagcc tttttgccgg
     2821 agcatgtttc ttcattcaag tttgtgccac aggaggggca tatgcctttg caatcgtccg
     2881 aacacagaat tgtcacgggc atggagagcc aaatgtcctc tgccagcaaa tcatcgagat
     2941 taagctgata agaagggata tacagacgat tgtcatcgta atccggttca ttctcagcct
     3001 catcttcatt gccgattacg agtatgtgct cggaaggcac agtcattgac cttactatgt
     3061 ctgaggcaca tctgtcgcac gcggtggtca gagtaaactc cgactgcgcg gacaaataaa
     3121 caatgccggt gcggttaaag attttgccct tagcatgaat taatgaaaag ggattttcct
     3181 catccgcgga aaagtaatcc gacattgaga attcatagtc aaagggcaga ctaaatccgt
     3241 cgttattaaa taccgatgtc agatcgactg tcatgcccat cactcctaaa atgcgtacag
     3301 aactcattat atagtcacat attgcttttg tcaacaaaaa aatacatcga aagatagtaa
     3361 gccgcgcatt cttttttgta aaaagctaac aaaagacaaa agccctttta tgcatacaat
     3421 tcaattgatt ttataagcgc ctcagacctt gcggaaataa ttcttaaaat caaagtatcg
     3481 gtctgtgtct ttacggggca gattttcata ttgcccaccg tagtgccttc gtaaatactt
     3541 atcttattat ttgcaaaaga gccgtcaata atctgaaaac cttcaattct ttgccctttt
     3601 tctatttctt ctttaataac cacataggat attttctgct tggcgggcag ttttataatg
     3661 tattcgcact gacttttgtc cgcgtttttg tcgactcttt gtatcttcgc gttttttgca
     3721 agattgtttg agaaagtgtt tctgattttc tcccccagct ctttaagcct tttaacatcc
     3781 ctttcatcaa acctcccgtc gggggtaggg ggaatattca gattaaatgt gcagttgccg
     3841 ccgaccgacc ttatataagt atcaaagagc ttatcgagga agtggggttc ttcctcctta
     3901 tgatagaacc atcccttgcg gattgacata tccacttcgg aagggcagaa cacaagcccc
     3961 tttgagtaca ttatgtttga caaagagccg atattctggt cgcagttata catataagaa
     4021 aggtcgcctt ggatggggga gtcaatccaa tgaacttctt tgtttctgtg gcataattca
     4081 tagggcacaa ccgcccattc ggcatatctt gaggagccct gctcgtttcc gcaccatctt
     4141 atatcgggac cgaaatcgct gaatatggcg gcattcggct gatatttgtt tataagctct
     4201 atatagccgt caaaatcata cacctgcttt tttccgttcg gaccctctcc gcaggctccg
     4261 tcaaaccata cataaaaaat ctcgccgtac tgagtgagaa gctccgtcag ctgagctttg
     4321 aaataatcat tgtaagcgtc ggtgccgtaa gtgggagcgt tcctgtccca tggggagaga
     4381 taaaagccga actttatccc gccctttgcg caggcatccg cgcattcctt tacaacatcg
     4441 cccttgccgt ttctaaacgg actgtttctc acgctgtgct cggtatattt tgatggccaa
     4501 aggcaaaagc cgtcgtggtg ctttgcggtc agcactattc ccgtcatgcc cgcggacttt
     4561 accgcctcca cccattggtc gcagtcgagt ttggcggggt tgaaaatctc agggctttct
     4621 ttgcccgtac cccactccat gtccgtaaag gtgtttactc caaagtggac aaaggcatag
     4681 aaattattat ttctcagcat attaagctgt ttgccgttag gtctaacatt cgcggcggtt
     4741 cttaataaat ctcgcatatt ttgctccccc tgtcttaaga acattttacc tctttttata
     4801 aaaagctcaa tatgttaaac tgaatatagt atttatttgg atttaagagt taaaataatt
     4861 tggacttgct ataattataa atatgttata taatatcaaa ggcttgaaaa caaaaagcga
     4921 ataataacag cagcacccat tttctgtcat gcggcgtgca ggtcctgtgc ggcggagcgc
     4981 cgcgaaccat gtcaggcggg gaaccgagca gcattaagcg gtatgcccgt gcgccacagc
     5041 ccgcctgtac gccgtatgac cgagagtggg tgttctttca ataaaatata aaaaggtaaa
     5101 ggctggaatt ccccctttgc ctttcatttg caggatgggg agggacttgt tttgtataag
     5161 gtattgtaca gaaagtggcg tccatcctct ttttctgatg ttgtaggtca ggaacatatt
     5221 actgttacct tggcaaacga ggttaaagag aatagattgg ctcacgcgta tctgtttacg
     5281 ggctcaaggg gaacgggcaa aaccacctgc gccaagattc tctcaaaagc cgtaaactgc
     5341 cttaatccca aagacggaaa cccctgcaac gagtgcgaaa tctgcgtcgg catcgacaag
     5401 ggcacaattc ttgatatcgc cgaaatcgac gccgccagca acaggggtat tgacgatata
     5461 agagctttga gggaagaagt atcatttact cccgcaaagg caaaatacag ggtttatata
     5521 atagacgaag tgcacatgct cacaatcgag gcattcaacg cgcttttaaa aacccttgag
     5581 gagccgccct cacatgtaat ttttatactt gccacaacag aggtgcacaa gctcccgtca
     5641 acaattattt cccgctgcca gcgttttgat ttcaggcgca tttctcccgt cagcatcgca
     5701 gaaagacttg agtttatcac agagtcggaa ggcgccaaaa tcacccgcga cgcggctctg
     5761 cttattgcca gaattgccga tggtggtctt agagatgcca tctctctgct tgacagatgc
     5821 atttcggtgt cgtctgacat caccgccgat gttgttgcaa aagccgcggg aattgcgggc
     5881 agggattatc tgtttaaaat ggctgatgcc atctgccgca aggatgtcga ttccatcctt
     5941 tcaatacttg acgaactgca caattcatcc cttgacagcg acagacttgt aaccgagctt
     6001 gttgcccact ttagggatat aatgatagtt aaaaccgtag aaaaccccct tgaccttatt
     6061 gtctgcacgg aggatgagct taagacttta aagtctttgg caaactccct tacggacgag
     6121 gccattttca gaattatcaa tatcctaacc gaaacagctg aaaagctgag aagaagcgct
     6181 aacaggcggg ctttggcgga gctggcttta ataaagattt gctccgaaaa ggacgaaaaa
     6241 gatttagaca gcctgcttgc aagaatagcc gctcttgaaa aggaattagc ctccttaaaa
     6301 cagaacggtt ttgtttcagc gccgcataca gagcccgcct ataaagcgga aaacaagtta
     6361 atacagaaaa aagcagagga cgctcatcaa acagcagctc aaactgtaaa tgatgacgct
     6421 ttcggctttg aggaagaaga ttcccccgag ccttcgccct ttgagcttga caatgagccg
     6481 tccttttttg atgagtcgtt taaaaatgaa attgataccc ctccctttga tgcggatatt
     6541 gcccctgtcc ctgaggagga atctatcccc tttgacgagg gcggtcagga tttgcctttc
     6601 ggagagggta gcctaattga ccttgagcct gacatttccg aaattcctca aaacgatgac
     6661 tttgcatatg ccgaaacaga cgcgcagccg tttgcggggg actcagcaaa gacggcggag
     6721 aaaaacgaaa cttcggacat tgttttaacg ccggaaatgt ggaataatat aacttatgag
     6781 attggaaggc agtgcccgcc cttaacgggt atactcggct ccatcagcgg ttatatggag
     6841 aatggagtgc ttgttataaa aatcggcaac gccttgttct gcgacctttg ctcaaccaaa
     6901 ttctcggagc agattcacaa ggctgtagaa aaggctgcgg gcaaggctat aagagtaaaa
     6961 tttatataat gagaatatag tttttcggag gtaaaaaatg aaagccagaa ttccaaacgc
     7021 agcaggcgcc aacaacatga tgcagagaat ccagaaaatg caggaggaaa tggcggctgc
     7081 tcaaaaagcg gttgaggaat ccgaattcac cgcaagcgca ggcggcggtc ttgttgaagt
     7141 gactgtcaac ggcaagcatg aggttacatc cgttaaaata aatcccgagg caatggaccc
     7201 cgaggagccc gaaatgcttg aggacttcct cattctcgcc gtaaacgagg caataaacaa
     7261 agcttcagac gccatacagc aggctatggg tcagattacg ggcggactta atatccccgg
     7321 catgccctcg ctgttttaat tatgagctat aatgcgctgc cgcttcagcg gcttatcgag
     7381 cagtttgaaa aaatgccggg catcggcaaa aaaactgccc agcgattggc attttatatt
     7441 ctttcaatcc ctaaggaaga agcccttgag gttgctaacg caatctacga tgcctgcgag
     7501 aagattaaaa gatgctccct gtgcatgaac cttacagagg aggagctttg ccctatctgc
     7561 cactccgatg aaagggacaa gagcgtaatc tgcgtcgttg aggacgctca ggatttgctc
     7621 gccatcgaaa aaacagggga gtacaagggc gtttatcatg ttttaaacgg agcgatttct
     7681 ccccttgacg gcataggtcc cgaacagctc agcatcaagg agcttttaaa acgccttgaa
     7741 tcgggcgttt cagaggttat cctcgccacc aactcggaca tagagggcga ggcaaccgcc
     7801 atgtatattt caaagctgat aaagcccttc ggcattaagg ttacaaggct tgcctacggt
     7861 ctgccagtgg gctccgacct gcaatatgcc gacgagctga cactttcaag agcccttaaa
     7921 ggtcgacgca cattttgatt ttgttttctt tggcataaat taataaatgc tcggagggaa
     7981 tgcaatgcaa agcgtctatg taattacata cgacatcgga accacaggtg taaaaacatg
     8041 tgtctttgaa atcggctcga aaattacact caaaggcttt gcctcggaag gctacggtct
     8101 ttatgttctc cccaacggcg gtgccgagca agaccccgac gaatggtgga ccgcaatgtg
     8161 caataccaca aagaaggcat taagcgaggc ggtagtcagc ggagacgaca taagcggtat
     8221 ttccttctgc tctcaaatgc agggccttgt actcgtagac aaagacggaa aagcattgcg
     8281 ccgctccatg agctatatgg accaaagggc ggaagctgag cttaaagagg gcattgccca
     8341 cggacttcaa atcgcgggcg caaatgtatt caaactctta aagtccctca aaataacggg
     8401 tgccgttgcc gcatcagtaa aggacccggt ctataaatat aaatgggtag aaaagaatga
     8461 acccgaaatc tttaataagg tttataaatg gctggatgtt aaggaatact taatttgccg
     8521 catgacaggc gaatttgtta tgacccacga ctcagccttc gctaccctcc tttatgatat
     8581 tagaaagggc aaaaggtgct tcagcgaggc aatgtgcaaa atgcacggcg tcaatattga
     8641 ccatctgccc gaaattaaga actcaaccga caaggtaggc acacttaaag ctgacgccgc
     8701 aagggagctt ggtcttaaag agggaatagc tgtattcggc ggcggcggcg acgcttcgct
     8761 tatcggcgtc ggcgcaggct ccgtaaacct tggcgatacc catatttatt caggcacatc
     8821 aggctgggta tccaccgttg tggataagag catagttgac gcgaacgcca tgatcgcggc
     8881 catagtgggc gctcaggagg ataagttcaa ctactttgcc gagcttgaaa ccgcgggcaa
     8941 atgccttgaa tgggtaaagg accatcttgc ccttgacgaa ataggcattt accttgaaaa
     9001 ggtttcggtc gtggattcat acgaaagcgt ttttgtaagc ctttacgact atatgtcgag
     9061 cgttatagac aaggttcccg caggcagcgg gggagttatc tttaccccgt ggctgcacgg
     9121 caaccgctgt ccctttgagg acccgaacgc aaggggcatg ttctttaata tgagccttga
     9181 aacaggcaag tcagagctta tcagagccgt cgttgagggc gtatgcatgc atctcagatg
     9241 gttccttgaa actcaggaga aaaaggtcaa gacctccgag gtcatccgct ttgtgggcgg
     9301 cggcgcattg tccgatgtga catgccagat tctcgccgac tgcacgggca gaaagatcga
     9361 aaccattgag aatccccaga acgctggcgc cgtcggcgcg gcggtactta tcgcggtggg
     9421 tctgggactc attaattcca tctccgacgc ccgctcactc atccccgcaa caaagagctt
     9481 tacccccgac agcaccgtaa agtctgttta cgacaagcac tttgaggtct ttaagaggct
     9541 ttacgcggat aataaaaaga attttgctga cttaaactca aaaaccaatt aagcaggaaa
     9601 ggtaaggaaa taaatgattg atttaaatac cacagccaaa ataattcacg aaggaattct
     9661 tgagacttta aagtcgcttt cctttgagcc ggtttacgaa aagggagccg acagaaaaaa
     9721 tccccctctt aaaaagaggg atgaggctca tcttatgcag tacgaaggcg aaaagggaaa
     9781 actcagaatc cttttttccg gcagcaaggt tttccttctt gtagcggctc ccgacgccga
     9841 aagcgaggac gactccgact acaaaaaggt tcttacgggc tttgtagatt atgacgagta
     9901 caccgaaaag gatatccgct cattggcaaa cgatattatc gatgaggtcg agaacaactt
     9961 tggcgtaaaa tccttcaggt ctcagaatgt caaaatgccg actcccgttt caagaactca
    10021 ggcaaagagc ggcgttatga gctacaatcc cttgaccctt gctaacaggc tgctctcgat
    10081 gtatcccgag cttaaagagc cttacaagca gaacattcag gattacggcg atttcctgcc
    10141 cgaggatttc ttcttaaacc acgcaaataa gtatattcat gaaaccatag agcagaacga
    10201 cccgcagaag atgaaaaagc tcttcggcat cctcaacgag atttttgagg acggcacgaa
    10261 cgaaacccag ggcgttatag ccgtaaccat actcggcgca aaatacaacg atcccgactt
    10321 tatgcagaag attgttgagt atatggaagg cggtctcctt gaggctgttg tagaagtcaa
    10381 caagtacctt gaaaagaaca ggggagcgag actcaggctc gaaaagccgc cgatttacaa
    10441 gcccaaaagg gacaaaaagg gcggacttat ggatttgctt ttgggcggtc agccccagca
    10501 gtcaggcaga taaaataaag gagtgcaaca gcactccttt taagttttag aggcggtaag
    10561 gcttctttta aggatgcgca aaaaaggctt agtcaatgtc cttggcattg gcataaacgc
    10621 ttgcacagag ctttcggtgc catcccttca cgctttttta aaggcggagg gctttcccga
    10681 cattgaaata ggcattgaca gagaaggcac tgatttcccg ggaaaaaaca cataccagca
    10741 ccgccttgcc gcaatggagg taaagcacaa aaccaatgat gaatgccttg acgctgtaaa
    10801 gttgtataga aaagttctta gtcaagcaga tgagaaagtg gatatagcag aaataggctt
    10861 tgaaacaata cttgcggggc ttttaaagtc aaatcccgat gagttttcgc cccttagcgg
    10921 catagagctt ataaagcaga aagtaaacaa gctgtggctt atggcgggca agtgggatga
    10981 cctttctacg ggatatgagt ataatttctc cgcaaatcaa agggcaagag tcgcagcgtc
    11041 atatgtgtgc gacaaatggc ccacggaaat aacctatttg ggctttgaag tcggtgaaaa
    11101 ggtcataacc ggcggctctc ttttggacgg ggatatactt aaagatgttc tcaccgccta
    11161 cggtcacccg agcggcaggt catcatggga cccatgctgg ttttgcttgc ctgcataaat
    11221 gatgaagaaa aggctggata ttatataaaa agaggacgcg caagccttga tatagcaact
    11281 ggctataacc actttgtttt tgacgctaac ggcccccacc gctttgtaat aaagaaattc
    11341 cccgacagct tctatgccga tatgataaag aattaattaa gagtgaataa aaagagcatc
    11401 tgtcaaaaga cagatgcttt aatatttatc agtgacttta agaagtttta gcggaaataa
    11461 taattccatt cgggcagata gtaacctata aactgttttt ccaaatattt tgctttttcc
    11521 gccgcttcct catcctcacc caaaaccaaa acaaatgtaa caatgtcctc taaatgtttt
    11581 ctaatcttgc ggtctgaagt gtgagtcctt acaccgtcgt tgtcgcttcg gtagtcgccc
    11641 agtttctttc taagtccccc attattccac tcggtcgcgc gtccgatata cataacttgt
    11701 ccgccgataa catgtttata tagccccact ttgttattaa aaggcgctaa gtttgcggtt
    11761 gagagcctgc cgatactgac ccatttctta tcccacgcgt caagatcgtt tatgtcgggg
    11821 ccggtgtctt ctttatcttc gctgagtctt gtttttatca tttgaagcct tagtatttgc
    11881 ccctcaatat tagtcccgct tctttcggca gcttttaatc tccgttcata gtcttcgtcc
    11941 ggtacatctg ttttgttttt tgagtttaaa acattataaa ataaatcccg aattcccaaa
    12001 aatacctccc ccttaaattg aatttaaagt gaattaagac ggcacctaaa taagatgccg
    12061 tcttaaatct ttaataagga ttatttaatc tgtcgccttc aaatacgctg tagcccaatg
    12121 caaccttctt aaagagccag ataagcggat tgaagcggaa cagaaccaaa tctacaaggt
    12181 ttataagagc gcggagagct gtggaaatat cctgtgttac gggaacctca acgggctcaa
    12241 actcttcgcc ttcaacattc agtacaaagg gctgagagta gcaaatgccg ttttctccag
    12301 tgatgtagaa ccttacatag agataaggct catccaaaag gttgtcggag tgcaaatcaa
    12361 gagttgcctt accgtctgta atgccgccct cacggagaat cacattgccg tttgaaaccc
    12421 atgtaatctg gttaaagttt gttccctcaa cggttatcgt atcattctcc tggtctactg
    12481 ttatgcgggt aaccatgggg gtattgtcaa ggagataaag ctcctcgtca taaaccctct
    12541 gctcgtcaaa gccggggatt tcctccatgc cgttcagctc aacgccgttt gaataatggg
    12601 ataccgcaaa gcagtgtccg tttaccatgg cgtagcgcag accatcaagg ctgagctcag
    12661 gcatcattac cattgtgtat gcgtcgttga ttgacctttc gttgtgggag tcggcgaaag
    12721 agaagcccca tggcacgacg ccgttcggaa ttgtcttttg cagaatctgg tcatacagaa
    12781 ttctgtcgca gcgggtgtgt gcgtcggtcg cgctgtttat gcccattcca acgcatgaac
    12841 cctgattgtc gaggaaaatt cgggcgaact tatttacata gtactcgtca atctttttgc
    12901 ccacatgctt ctcggtgtct ttattgatgt aaacatactc tcccacatga gcgagcattg
    12961 agatgccgcc tgcgttttta actccgcggg caggagtttc gtagtctccg aatacgcccg
    13021 caaggccctg accgtaggtt gagaaatagc ctgtcaaatg gcagtcggct atgggagtcg
    13081 ccatattaag ctcaatgcca aggggcacat ccagcatgcc gtttgtcttg gttctggtgg
    13141 gagagggagc ggtgccgtta atgtaagaat aataaacctc atctgaaagc ggcacaatag
    13201 gctccatttt tgttctttcc ctcttaacca aacggataag cggaactgtg tcgggcatct
    13261 tgttccagcc cttgttcagc gtgccgtggt cggtcaaagc aactatgtcg tagtttaagt
    13321 catagtgctt ttggacatat tcatcaatct tcaaaaagcc gtcgcttgcg gttgtgtgag
    13381 aatggggctg gaatttatac gcgccccaag aatcccaatc gacttcggca tatgggtcgg
    13441 tgattatgta attcttagtg cccgcgccaa aggcgacaag cgaaacagcc tggaatatga
    13501 gcgcactgca gagaacaatc gccatcagct tctttttcat cacagcatct cctctcatgt
    13561 tatcacaaga gttagtatat aatagccaaa aaataaatgc aaggacaaaa aatattatat
    13621 acttttccgt tttttcatag ctggaggcaa aagttatttc gcaaagagct tgctaatatc
    13681 ttttggagca ataatgccgt caggagtaat aaacgcggtt atcagctccg cgggagtgac
    13741 atcaaaggcg ggattatata cttttgcctt atcgggcagc atgctttctt tatagtgcag
    13801 acttctgatt tcagaatccg ctctttcttc aataacaatg ccgccgccgt cgggagtgtc
    13861 tttgtcaaag gttgaatatg gggcgcagac ataaaatggc acgccgaaaa accttgcgtt
    13921 aacggcaagg gagagggtgc ctattttgtt tgctgtgtcg ccgtttgcgg caatcctgtc
    13981 ggcgccgaca aatattgcgt ttaccttgcc ctcagacatt acctttgccg ccatgttgtc
    14041 gcagataacc gttgtatcaa ttcccgcgtt tgtcagctca aaggcggtaa gtcttgcccc
    14101 ttgcaagagc gggcgggttt cgtcggcaaa aacctttatc tttttccctt gttcagcggc
    14161 aacataaaca ggggagagtg cggtgccgta tttaaccgtg gcaaggctgc ccgcgttgca
    14221 gtgggtcagc acagtgtcgc cgtcttttaa aagagcggct ccgtgctcac cgattttcct
    14281 gcacatctca atatcttcat caataataaa aagagcctcg ttttttactg cttctttagc
    14341 cgaatcaatt ccgtcacgaa gggcattttc gagagccttt tccatcctgt caagagccca
    14401 gaagagattt actgccgtgg gtcttgattc tctcagccta ttctcaataa agtcagcctg
    14461 agatttaaat tccgttttat ctgaggtttt tatcttattt acaagcaccg aatatcccac
    14521 agcggctgcg gctccaattg cgggtgcgcc cctgactctg agagagcgta ttgcctcgaa
    14581 aatatcctca agctcatcga gatacaaaag cctatactcc cttggcagga gagtctgatc
    14641 aactattaca agcagattgt tctcagtatc atacgccaaa gtagcgccgt ttaagtcctt
    14701 tatcataatt caccaactgt tgtttcaata agttttccga gcttttcgcc gcttcttctg
    14761 cctatttcgt tcacttcctc acccgaaagg cattttccgc tgattcccgc ggccttgttt
    14821 gtaataagtg aaaaacccag tacctcaatg cctgcgtgaa cggcggcgat aacctcaaat
    14881 actgtggaca tgcccactgc gtcagcgccc ataactctga aagcccttat ctccgcggga
    14941 gtttcaaagc tgggacccgt aacgccgata taaacgcctt ctctgagttt aatgccgagc
    15001 tgttctgccg cttcctttgc cttttctctg agccgcggtg cgtaagcata agtcatgtcg
    15061 gggaatcttg tgccaaagca gtcaagattc tttccgataa ggggatttgt gcctgtaagg
    15121 ttaatatggt cggttataag cattatgtcg cccacatcaa agttttcatt tattcccccc
    15181 gcggcgtttg ttacgataag ggttttaact cccagctctt taaagagcct tatgggaaat
    15241 acgatatcct ccttgcagtg accctcataa taatgtaatc tgccctgcat gcagattaca
    15301 tccttgcccg aaagtttgcc aaaggctagc ctgcccttat gccccggcgc tgttgaaacg
    15361 gggaatgcgg gtatatccct gtaatctaaa aatacagcat cctcaaggtt ttcggctatg
    15421 tgattcagac ccgagcccag aataattgct gtatcggcgt tcgttttaac cttgccttct
    15481 atatatgttt tcgcagcctt taaaagctcc agcatatctt cactcctaat tgtcaagctc
    15541 tgaaataatt ctctttacag acctttcagc ctcgtgcttt accttttcta tatcaaatga
    15601 gtaatactcg ttgttgtaat aaagtatcct gccgtcgacc attgtcataa ggttttcacc
    15661 gcccgacagc gcgtaaacaa ggttgcttgc catgtcagtg gcagggcgca tattgggcca
    15721 ctctgtgtca atcattctca aatccgccct gcagccttcc ttaataacgc ccgtgtcggg
    15781 cctgttttga gctttagccc cgtttacggt ggcagccttc
//
LOCUS       DAONVQ010000032        15799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_2767_length_15799_cov_4.906250, whole genome shotgun sequence.
ACCESSION   DAONVQ010000032 DAONVQ010000000
VERSION     DAONVQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15799
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2767_length_15799_cov_4.906250"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..1360)
                     /locus_tag="PLD98_03385"
     CDS             complement(290..1360)
                     /locus_tag="PLD98_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA05326.1"
                     /translation="MKKLKIGLIGIGGICNGVHIPAYLQDERTEVVALCDIVPEKTAK
                     AKKEFFPNAKEYTDFRELLKDDSIDAVDICTPNYLHSIIAVEALERGKHVFCEKPDAV
                     SVGEVQRMKAAAEKSGKVLMVMRNNRFMHISKLAKQFVEEGRMGEIYAGRCGWIRRRG
                     IPGKGGWFTTKEMSGGGPLIDLGVHMIDLAIWLMGNPTPVAVSGATYRKFADSDISDS
                     PNSKFGETKEGGTFDVEDLAMGFIRFDNGAVLQIEFSWASNIEKETRFVELRGTEAGL
                     SLDPEKLRLFYEKDGKLINKTFKKVIGKGKDGHAGNIENFVDVVLGQAEPVFVPQQGV
                     DMIKILTAIYESAEKGREVVLK"
     gene            1860..2636
                     /gene="spo0A"
                     /locus_tag="PLD98_03390"
     CDS             1860..2636
                     /gene="spo0A"
                     /locus_tag="PLD98_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HQA05327.1"
                     /translation="MSKQIKVLLADDSAEFGQACCSVLRSHGFDVFLSAKNGNEVLAS
                     IKSTQPDVVLMDTFMPHLDALGVMKQMNNMRLAKRPLIFLMSSVDNATFEKEVLAAGA
                     DYYFLKPFDIDMVAERITQLTGWQKNGIATTRTNGRNLEHDMDVIVSDIMHQIGVPAH
                     IKGYHYLREAIKLSIQDTEIMNSVTKLLYPTVAKMFSTTSSRVERAIRHAIEVAWDRG
                     DVDILSSYFGYTIQNSRGKPTNSEFIAMISDKLRLRMKIS"
     gene            complement(2713..3552)
                     /locus_tag="PLD98_03395"
     CDS             complement(2713..3552)
                     /locus_tag="PLD98_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05328.1"
                     /translation="MFAVLELYKVGNSFKEKLRKRFLKPSISVDRLSIYDGMHYYIIR
                     APIIDGEIDWKGIGEAAGGHKKRMLIPHGVEVPEDSGIVRFKPRVFQRIILFNTAVSL
                     LALSDLIPSETVISVIDYDMLDTERIYSLLPYASIVKVVTRESNIEAYNCFSDNLYNE
                     TGARLIVTDDISAVKNSNLILTAEEVEDENLSKCRVLSFYPLNNTPKALCIGCIELTH
                     SLNLPFYEGIPMIDFLGALYELCPPSERLTPIRAKTLITDHFELTIFSAANLLSKDLE
                     NEQ"
     gene            3654..4355
                     /locus_tag="PLD98_03400"
     CDS             3654..4355
                     /locus_tag="PLD98_03400"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HQA05329.1"
                     /translation="MKNLRKHLFSLADEDYKNFSIKLLPENTNMIGVRLPQLRKTAKT
                     MVKNGLGSELLSFIYDYECEYFEETMLKGMAIGAVKCGLEEKLKLIRHFVPYIDNWSV
                     CDSFCSSLKFDKAELPKVWDLIKEYTESSEEFFARFGYVSIIRFFINEEYIDRVFERF
                     EALKSPELYARLAVAWAISMCFPDFEERTLEYIRASTLDDFTLKKAVGKIKESYRVSS
                     EGKQRAVKALAERKK"
     gene            complement(4493..6163)
                     /locus_tag="PLD98_03405"
     CDS             complement(4493..6163)
                     /locus_tag="PLD98_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HQA05330.1"
                     /translation="MVIEYMTQNKPEYNCYEYMEKWTETFGEYCVIERFGQEVYRSEF
                     LDQINRMAAYFENELGLKRGDVYTIFLPTIPQSFVAFYALNKIGVIISFVHPLSPPET
                     LKELMIESGSKGIILFDILAKKYVPVINELGVPALVCGLSDYAAPLRKAGLKVADAIM
                     GRHVRKLKKWDTYKNAISKYPPSKTKLDNGTDVAFYLNGGGTTGKSKTIKLSSYAINE
                     IVDKVVRIDGGRYSIEAPGIESTIVVLPLFHAFGLAVAVHMPICQGGRMLTMMDFNPK
                     RFNSMMRKNRVIFIVGIPVMFRKLMAEKNFDGPHLKNLRFLFCGGDDVNEMYLDQFNA
                     YLEKWGASGRLFRGYGLTEVASVCTTNTFENFRRHSIGKPLEGITVEIWDDECKKVPN
                     GTIGEIVISGSTVMDGYLDKDRTENLGVYIDENGTRWVRSGDLGYMDDDGFIFFSGRK
                     KRIIIISGYNVYPTDIEQKVEELPFIKEVCAVQGIVDCKPIIRLYVSFKGDNYDEEEY
                     KRQIFEKCEKTLPAYSRPREIVVMKELPRTHMKKIDFMSLTETMPKAQ"
     gene            6815..8602
                     /locus_tag="PLD98_03410"
     CDS             6815..8602
                     /locus_tag="PLD98_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HQA05331.1"
                     /translation="MKKNLLYKSIAVLLCILMLSIIPLSAYAASDSSKTPVILVTDII
                     ANEIVADVSNSQSEILYPKENNDINKLIRKFIDSYFMASEGHHDMAVDNFMSELNAAM
                     RPLALNPDGSLAFELSTVRSYSYRLDKPTTPASMYFYRNKAEVLDKVYGGIGRKIADR
                     IGDENVFAFNYDWRLDPAENAEKLNQYIQDVKFLKGVNNVTLVAEGFGGIVSSVYLHN
                     YAEANNFADIKNYVLVNSYFQGLGLVGDVFTGNIMVLDSALVRYVNDQPENYIAQFTM
                     WLTQYILNTEWELSHFTATITSTLLQRKARVYSYVKDIFKTVPGLWAMVPAAQFEDAK
                     QFMQFDPTINAQLSPMNPELLQKIDAYHEVQVAAPETLKKLQQSGAGVAIVAGYNLQN
                     FPITKYSAINQSDGMVDTVYASAGADCLKLNAAYLNIFDFLGKRNAQIVDTRYDNVSG
                     DRLIDASTCALPQNTWFIKNLKHNGFRYDSNSSDFIAWLATVESPNVWRSVKYPQFMS
                     YNKFTQKLNTPSSNSRPEGYLLGDVNLDGKVTAADARIVLRVAAKLEKESKLSALAKQ
                     NADVNLDGKIDIKDARLILRLAANLDLDQ"
     gene            complement(8802..9599)
                     /locus_tag="PLD98_03415"
     CDS             complement(8802..9599)
                     /locus_tag="PLD98_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05332.1"
                     /translation="MLYFSEVPILKPNLPESKVKKVLAGGIPPEAERELKLFGIEIIS
                     PEPCCDVLEPLKMHADMLCHIALDGKVFMSEGQESLERALSGLGFNISFAKAKLGREY
                     PLDVPLNAAFIGSALICRPSAVLPELLNYYRELGVKIIETQQGYSKCSVSIVAENAII
                     TEDESIYKACLKNNVDVLLIKKGYVNLPGFDCGFIGGASGLISKDCLAFCGGIENHPS
                     YKEIKAFAANYGVSLISLSREALCDIGGIIALTEIKGKDNENAGKEK"
     gene            complement(9602..10969)
                     /gene="radA"
                     /locus_tag="PLD98_03420"
     CDS             complement(9602..10969)
                     /gene="radA"
                     /locus_tag="PLD98_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQA05333.1"
                     /translation="MSPKSKTVYICSECAYESSKWYGQCPSCGAWNTMTEEVVASVKS
                     ASAAPKASSAVKAVNLMDVRTDTEQRFKTGSPELDRVLGGGLVKGSVVLLCGEPGIGK
                     STILLQTCDFLGSRFNVLYVAGEESVSQIKLRADRLKVFADKLYIINETDTQSVCEYI
                     LSAKPDVVIVDSIQTMSLPEISSTPGSVVQVRESASFIQRTAKALNIPVILVGHVNKD
                     GNIAGPKVLEHIVDAVLLFEGERNMSYRILRAVKNRYGSTNEIGVFEMGNEGLKDVVN
                     PSLLFLSGRPENASGTCIACIMEGTRPILAEVQGLIAKSAYGTPRRMATGFDYNRMSM
                     IIAVMEKRVGLNFGECDSYINVAGGIRIYDTSADLPVALSLVSGFKNVVINHDLVAFG
                     EIGLAGEVRSVQFCEKRVIEAMRLGFRHCIVPYHNAVSLPAELKRQINIIPVKTIADA
                     IKVIS"
     gene            complement(10987..11700)
                     /locus_tag="PLD98_03425"
     CDS             complement(10987..11700)
                     /locus_tag="PLD98_03425"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQA05334.1"
                     /translation="MKRRIVIILFSLAALAAFFALSVFIYYQSGKLGIPVSADGSEPI
                     IIVDAGHGGFDGGAVADDGTVEKDLNLQIALKLNDILRMAGYKTILTRTDDTPTSDPD
                     AKTIREKKHTDLVNRMKLIEETPNAYFVSIHQNMFGVSSYWGSQVFYSGNNPESEILA
                     KNVQQSIRELLQPNNKREIKKSGKSIYLLYYAEKPAILIECGFMSNGPELAKLKNEDY
                     QKKLAFAIFCGINEYFNSP"
     gene            complement(11780..12895)
                     /locus_tag="PLD98_03430"
     CDS             complement(11780..12895)
                     /locus_tag="PLD98_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05335.1"
                     /translation="MDEKEKNITPENNSANTEKDPLEEILESLGLSRKTTDGKPSYFS
                     EKKAAEPTEPAKPTVEEKKEEIKEEPAAAEPEKEKADEKKPAQDLSRYEQKPKVQEFH
                     SAKDKKEPAAMRRNVAFFFGVLIVLFSLFGFYNAAKLAVGGIVSLFDNSDKIAMYNEF
                     LVPVVANDPEPFDDIAMANSADLIDISLWAILKGDLTPDQYEYRDGFMIIPAADVEKR
                     FAALFGTEVKPVHQTITNFGYEFVYDKADAVYKIPLTGMSPIYSPRVTDIEKKSSSVI
                     LTVAYIGGESWTQDKQGNFIPAEPDKYMSITLREVNGEYHISAIQAKNAPETAATLPS
                     RTETQAPQPVTTTEASAPENEAQDTSEESETAENTAE"
     gene            complement(12912..14159)
                     /locus_tag="PLD98_03435"
     CDS             complement(12912..14159)
                     /locus_tag="PLD98_03435"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HQA05336.1"
                     /translation="MSNAVLEIGKKAAAAKSSIANAGSAEKDRALRLIAEALIRDTDK
                     ILAANEKDVNAAKENGMSSALIDRLSLTKDRIKAIADAVLEIISLPDPVGRVVSGSTR
                     PNGIRITKVSVPLGVIAVIYEARPNVTADAAALCLKSGNAVILRGGKEAFNSNMEIVN
                     SMRSALSQTSISPDCIQFIEDTSRESATALMRLNGYVDVLIPRGGAGLIKSVIENSTV
                     PVIETGTGNCHVYVDKSADIDMATEIVFNAKTSRPSVCNAAEKLLIHKDIAQTALPKI
                     CEKLKEKNVEIRADESALKLIPDAKPATDEDWDTEYLDYIIAVKAVNDIDEAIKHINT
                     HGTKHSEAIVTESFAAAEKFCAEVDAAAVYVNASTRFTDGGEFGFGAEIGISTQKLHA
                     RGPVGLPELTSYKYVVRGEGQIR"
     gene            complement(14179..14964)
                     /gene="proB"
                     /locus_tag="PLD98_03440"
     CDS             complement(14179..14964)
                     /gene="proB"
                     /locus_tag="PLD98_03440"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HQA05337.1"
                     /translation="MSTLKEAKRIVFKVGTSTLTYDTGKANIRRMSKLAEVLSDLQNS
                     GREVVLVTSGAIGIGVGKLGLKSKPSDTPGRQAAACVGQCELMFIYDKLFSEFGHTVG
                     QLLITKSDVDDPERCSNLINTFEKLFEFGAIPIVNENDSVAVDEIVFGDNDNLSATVA
                     KLIKADALVILSDIDGLFSANPLEDASATLIPCVDEIDDEIFSLASGAGTNRGTGGMI
                     TKLQAAKTATEAGITTVVMNGYDPTDIYKLIDGHSVGTLFRCK"
     gene            15255..>15799
                     /locus_tag="PLD98_03445"
     CDS             15255..>15799
                     /locus_tag="PLD98_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05338.1"
                     /translation="MKIKIAAVIGMGTTSQRNCDSFNINGRVPLMAEADGGYKAAGEI
                     STPFVIGAFSGSAVGSAFAAANMLTEHAEAIKLSEEEYKSSFLDYLERLRNEVIRREE
                     GIIAFSVALLYAYNDTIAVARLGKNRIYRFSGIELMSIPENRQSGGKQMSGGMDHYDD
                     IKTEDRFLILGPGVVNALTES"
BASE COUNT         3930 a         3680 c         3712 g         4477 t
ORIGIN      
        1 aactgagaaa atatatctta cggactgtct ttgttaaata taatatacaa tgtttcggaa
       61 tttgtccata tatatttcgt tgccgcaaaa agaagccgct gattctattt tgcccacttt
      121 atgctttaaa cccgaataat aatcgacaaa aaacgactaa tttctttgcg gaagtattga
      181 attggcagaa tttatgtgat aaaatatcta aaaattttca gcttggtatc acttttattg
      241 gttaaattaa agagcaagta cataaagtac ttactctgaa ttaagcttat tatttaagga
      301 cgacttccct gcccttttcg gcggactcat aaatggcggt gagaatcttt atcatgtcca
      361 cgccctgctg gggaacgaat actggctctg cctgacccag cactacatca acaaagttct
      421 cgatattgcc cgcgtgaccg tccttgcctt tgcctatgac ctttttgaag gtcttgttta
      481 tcagcttgcc gtccttctca tagaagagcc tgagcttttc ggggtcaagg ctgagtcccg
      541 cctcagtgcc gcgcagctcc acaaaacggg tctccttttc gatattggat gcccagctga
      601 actcaatctg gagcacggcg ccgttgtcga accttataaa gcccatggcg aggtcctcaa
      661 catcgaaggt gccgccctcc ttggtttctc cgaacttgga gttggggctg tcgcttatat
      721 cgctgtcggc aaactttctg tatgtagcgc ccgaaacggc aacgggagtg ggattgccca
      781 tgagccagat tgcaaggtca atcatgtgaa ctccgaggtc gattaaagga ccgccgccgg
      841 acatttcctt agtggtgaac catccgccct tgcctggaat tcctctgcgt cttatccagc
      901 cgcagcggcc ggcgtagatt tcgcccatcc tgccctcttc aacgaactgc ttggcgagct
      961 ttgagatgtg cataaagcgg ttgttcctca ttaccatgag caccttgccg ctcttttccg
     1021 cggctgcctt cattctttga acctcgccga cgctcacagc gtcgggcttt tcgcaaaaga
     1081 catgcttgcc cctctcaaga gcctcaacgg cgataatcga atggaggtag ttgggggtgc
     1141 agatgtcaac ggcgtcgatg ctgtcgtcct tcaaaagctc tctgaagtcg gtatattcct
     1201 ttgcgttggg gaagaattcc tttttagcct tggctgtttt ctcgggcact atgtcgcaga
     1261 gagcgacaac ctctgttctt tcgtcctgta aatatgcagg aatgtgtact ccgttgcata
     1321 ttccgccgat accgataagt ccgattttta gctttttcat tttcttaacc tctttattca
     1381 caaatttttt gattcgcagt cttttcatct gtcgcatgag ccttgattta cacagtctat
     1441 tttataaaaa cagggaaatt tttcaattct tttacttaaa aattatcgac ccgcagcccc
     1501 aaaatccttt attttccgac agcttgaaaa atttaatcga aaaatgtttt ttggtgtata
     1561 gcgtattttg tcggcgtatt tgggtggaaa atcgtgttta acaaggcgat ttaggggcga
     1621 aaaaaacaac atcttgcgga aattgtaaat ttagacaact atatgtagat tgacgaaaaa
     1681 tcgtgaattt tacagaaaaa taatggtaaa tttttacaaa aaataggctg tcagctattg
     1741 ccatatgccg taacctatgg taatatatgg gagaaatgag cacaggcaga tagcaaaatt
     1801 ctacaattgt ccattcgaaa ctgctgaagg ctggttaata gagaaaggat gagaaactga
     1861 tgtcaaaaca gattaaggtt ttgctcgcgg acgattccgc ggaattcgga caggcttgtt
     1921 gcagcgtgct tagatcacac ggttttgatg tatttttatc cgcaaagaac ggcaatgagg
     1981 tgctggcctc gatcaagagc acacagccag atgtggttct aatggatact tttatgcctc
     2041 atcttgatgc tcttggcgtc atgaagcaaa tgaacaacat gcgcctcgca aagagacctc
     2101 ttatcttcct tatgtccagc gttgacaacg ctacatttga aaaagaggtt ctggcagccg
     2161 gcgccgacta ttacttcctc aaaccctttg atatcgatat ggttgccgaa agaatcactc
     2221 agcttaccgg ctggcagaaa aacggcatag caaccacacg cacgaacggc agaaaccttg
     2281 agcatgacat ggatgttata gtttccgaca taatgcatca aatcggcgtt cccgcccaca
     2341 ttaagggcta ccattactta agagaggcaa tcaagctctc cattcaggac accgaaataa
     2401 tgaactctgt taccaagctg ctttatccca ctgtggcaaa aatgttctcc acgacttctt
     2461 caagagtaga aagagctatc cgccatgcca ttgaagttgc ctgggacaga ggcgatgtcg
     2521 atattctgag ctcttacttt ggctatacca ttcagaactc aaggggtaag ccaacaaatt
     2581 cagagtttat cgcaatgatc agcgataagc tccgtctgag aatgaagatt tcttaaattc
     2641 gggaaacctt gtttctgaat ttagattaaa tatttaaagc agcaggtttg actgctgctt
     2701 ttttatttgc ctttactgct cattctccaa atctttgctt aaaagatttg ccgccgaaaa
     2761 aatggtcagc tcaaagtggt cggttatcag cgttttggct ctgatgggcg tgagtctttc
     2821 cgatggcggg caaagctcat acaaggcgcc gaggaaatct atcatgggta tgccctcata
     2881 aaagggcaga ttcaggctgt gtgtcagctc aatgcagcct atgcacaaag ccttcggggt
     2941 gttattaagg gggtagaagg acagaactct gcattttgaa aggttttcgt cctctacctc
     3001 ctcggcggtc agaatcaggt tgctgttttt gaccgctgag atatcgtcgg ttacaatgag
     3061 ccttgcgccc gtttcattat atagattgtc ggagaaacag ttgtacgctt caatattgct
     3121 ttcgcgggtt acaaccttta caatagaggc gtagggcaaa agggagtaga ttctctctgt
     3181 gtccagcata tcgtagtcga tgaccgatat gaccgtttcg gagggtataa ggtcgctgag
     3241 ggctaaaagg cttaccgccg tattgaaaag gatgattctt tgaaataccc tcggcttgaa
     3301 ccttacaata cctgaatctt cgggaacttc cacgccatgg gggatgagca ttcttttttt
     3361 atgtcctcct gccgcctccc ctatgccttt ccagtcgatt tccccgtcga ttatgggcgc
     3421 tctgattatg taatagtgca ttccgtcgta tatggacagc ctgtctacgc tgattgaagg
     3481 ctttaagaag cgttttctca gcttttcttt aaagctgttc cccactttgt acaattccag
     3541 taccgcaaac atatatttcc ccccaaattc gctgttttaa tatgtatatg agctttgggg
     3601 ctaaaactct acaaagtatt tacaaagcaa aggtattttc aaaaggagaa ggtatgaaaa
     3661 atctaaggaa acatctattt tctcttgcgg atgaagatta caaaaacttt tctataaagc
     3721 tgctgccgga aaacacgaat atgataggcg tcaggctccc gcagcttaga aaaacggcta
     3781 agaccatggt taaaaacggt cttggctctg agcttttgtc ctttatttac gactatgagt
     3841 gcgaatattt tgaggagaca atgctgaagg gcatggcaat aggcgcggta aaatgcggtc
     3901 tggaggaaaa gctaaaactt attcgccatt tcgtgcccta tatagacaac tggtcagtgt
     3961 gcgacagctt ttgcagttca ttaaaatttg acaaggcaga gctgcccaaa gtgtgggact
     4021 taattaaaga gtacactgaa agctctgagg aattttttgc ccgctttggc tatgtttcta
     4081 ttatccgctt tttcataaac gaggagtata tagacagagt ttttgagcgt tttgaggctc
     4141 taaagagccc agagctttat gcccgccttg ccgtggcgtg ggcaatctct atgtgttttc
     4201 ccgattttga ggaaaggact ttggagtata tccgcgcttc aacgcttgat gatttcacct
     4261 taaagaaggc ggtcggcaag attaaggaat catacagggt ttcatctgag ggaaagcaaa
     4321 gggcagttaa ggctttggct gaaagaaaaa aataaaaaac ggcagagcta attgcttctg
     4381 ccgtttttct ttattattgc acaggcgcaa atctgaggtg ctaatataac ggaaccatcc
     4441 ccaaataaac ttcccaaagc agcgctccgt cagatttcgc cttatgcatt atttactggg
     4501 ctttaggcat tgtttctgta aggctcatga agtcaatctt cttcatgtgt gttctgggca
     4561 gctctttcat aacgactatt tcacgcgggc gggagtacgc gggcagagtc ttttcgcact
     4621 tttcgaaaat ctggcgcttg tactcttcct cgtcatagtt gtcgcccttg aaggatacat
     4681 acagtctgat tatcggcttg cagtctacga tgccctgtac ggcgcagacc tctttgataa
     4741 agggcagctc ttcaaccttc tgctcaatat cggtcgggta gacattgtag ccggagatga
     4801 tgatgattct cttctttctg ccggagaaga agataaatcc gtcgtcgtcc atgtatccca
     4861 ggtcgcctga gcgtacccat ctggtgccgt tttcgtcgat gtatacgcct aagttctctg
     4921 ttctgtcctt atcaagatag ccgtccatta cggttgagcc ggagatgacg atttcgccga
     4981 tagtgccgtt gggaaccttc ttgcattcgt cgtcccaaat ctcaacggtg atgccctcaa
     5041 gaggcttgcc gattgaatga cggcggaaat tctcgaatgt gttggtggtg catacggaag
     5101 caacctctgt aaggccgtag cctctgaaca gtctgccact tgcgccccac ttttcaagat
     5161 aggcgttgaa ctggtcgaga tacatctcgt taacatcgtc gccgccgcag aacaggaatc
     5221 ttaaattctt gagatgcgga ccgtcgaagt tcttttcagc cataagcttt ctgaacatga
     5281 cggggatgcc tactatgaag atgacgcggt tcttgcgcat catgctgttg aatctcttgg
     5341 gattgaagtc catcatggtg agcattctgc cgccctggca aatgggcatg tgaaccgcaa
     5401 cagccaaacc gaaggcgtgg aagagaggca gaacgacaat tgtggactca atgccggggg
     5461 cttcgattga atatctgccg ccgtcgattc ttacaacctt gtcgactatc tcattgatgg
     5521 cataggatga gagcttgatt gtcttgctct tgccggttgt gccgccgccg ttgagataga
     5581 atgcgacatc ggtgccgttg tcaagctttg tcttcgacgg gggatatttg gaaatagcgt
     5641 tcttatatgt atcccatttt ttaagctttc ttacatgcct gcccatgatg gcgtcggcaa
     5701 ccttaagacc tgcttttctt aaaggagccg catagtcgga cagaccgcat acgagagcgg
     5761 gaacgccaag ctcatttata acggggacat actttttggc gagaatgtca aagaggataa
     5821 ttccctttga gccgctttca atcatgagct ctttcaaggt ttcggggggt gaaagggggt
     5881 gaacaaagct gatgattacg ccgattttgt tcagagcgta gaaggcaaca aagctctggg
     5941 gaatggtggg caggaagatg gtgtaaacat ctccgcgctt taagcccagc tcgttctcaa
     6001 aataagctgc cattctgttg atttgatcaa ggaactccga acgataaact tcctgtccga
     6061 atctttcgat gacgcagtat tcaccgaatg tttcggtcca tttttccatg tactcgtaac
     6121 agttgtactc gggtttgttt tgggtcatat actcgataac caagaaactt cgcctcccaa
     6181 acgatagtcg ttttttcatt ttattttctc ttcggaagat tcccgaatca ccaaattata
     6241 acctttaggg aattaccttc ctaagcaaat gcaaaattat tgtaacaata tcgcaatata
     6301 ttgtcaacac aatttatcat aattgcataa ttaaattatg tcaatttcaa gatttatgta
     6361 ataattagtc aagtttcaat aattttctaa aaaatatgcc attttatgcc aattttatat
     6421 tcgactgtgc tcgacatttt tcaacattta cataatcggc aatcccgcgc tgtaatttgc
     6481 gctttttcga tttttatata gacttagaag ccgcaaaaga attatagccg gtttatagaa
     6541 aaaaatgtat agattccgga ctcaagctta agctgataac attttattca aacaatgtcc
     6601 acaaaagagg aaaacacagt attttgaaaa tagtgttaac aatatgttaa tttttgggac
     6661 agggtagaaa ttttaccgca ctcgcttttg ttaagtttaa atttcggagg cttttccatt
     6721 atttgaaata ttttgcgcac ataaaaaata aacagttgaa atctgaaggg cgctgatata
     6781 taatgtaaac aatagaatga ggaggaatat gtgcatgaaa aagaacctct tatataaatc
     6841 aattgccgta ctgctttgca tattgatgct gtcaattatt cctctatccg cgtatgcggc
     6901 gtctgacagc agcaagacac ccgttatttt ggtaacggat attatcgcta acgaaattgt
     6961 tgctgatgtc agcaattcac agtctgaaat actgtatccc aaagagaata acgatataaa
     7021 caagctcatt aggaaattta tagactcata tttcatggcc tccgagggcc atcacgacat
     7081 ggcggtcgac aactttatgt cagagctgaa tgccgccatg agaccgcttg ccttaaaccc
     7141 cgacggctcg ctcgcctttg aattgtcaac cgttcgttct tacagctaca ggctggacaa
     7201 gcccaccact cccgcgtcca tgtacttcta caggaataag gctgaggtgc tggacaaagt
     7261 ctacggcgga atcggcagaa agattgccga cagaatcggc gacgagaatg tattcgcgtt
     7321 caactacgac tggagacttg accccgccga gaacgctgag aagctgaacc agtacataca
     7381 ggatgttaag ttcctcaagg gcgtcaacaa tgtaacactt gtcgcggagg gcttcggcgg
     7441 catagtttca agcgtatatc tgcacaatta cgcggaagca aacaactttg ccgacataaa
     7501 gaactatgtt ctggtaaact cctacttcca gggtctcggt cttgtgggcg atgtttttac
     7561 gggcaatatt atggtccttg acagcgcttt ggtaagatat gttaacgacc agcccgagaa
     7621 ttacatagct cagtttacaa tgtggcttac acagtacata ctcaataccg agtgggagct
     7681 ttcacacttt acagccacca tcacctccac gctcctgcag aggaaagcca gagtgtattc
     7741 ttatgtaaag gatatattca agactgttcc cggtctttgg gcaatggttc ccgcagccca
     7801 gttcgaggac gcaaagcagt tcatgcagtt tgaccccact ataaatgcac agctctcccc
     7861 catgaatccc gagctccttc agaaaatcga cgcataccat gaggttcagg ttgccgctcc
     7921 cgaaacgctc aagaagctcc agcaatccgg cgcgggcgtg gctatcgtgg caggatacaa
     7981 tctgcagaac ttcccgatta caaagtactc cgcaatcaat cagagcgacg gcatggttga
     8041 caccgtttac gccagcgcag gcgcagattg ccttaagctg aacgcagctt accttaacat
     8101 cttcgatttc ctcggcaagc gcaatgctca aattgtggat accagatacg acaatgtatc
     8161 gggcgaccgc ctgattgacg catccacatg cgctctgcct cagaacacat ggtttatcaa
     8221 gaatctcaag cacaacggct tcagatacga cagcaacagc agcgacttta tcgcatggct
     8281 tgccacagtt gagagtccga atgtatggcg ctccgtaaaa tatcctcagt ttatgtcata
     8341 caacaagttt acccagaaac tgaacactcc ctcatcaaac agcagaccgg aaggctatct
     8401 cctcggcgat gtaaaccttg acggcaaggt cacagcagcg gacgcaagaa tagtactgag
     8461 agttgccgct aaacttgaaa aagagagcaa gctctccgct ctggcaaagc agaatgccga
     8521 tgtaaacctt gacggaaaga ttgatattaa ggacgcaagg ctcatactca ggctcgctgc
     8581 aaatctggat ttggaccaat aaattaaagc ccccttaaac gggggctttt tttgcttccg
     8641 atttaaaaag cagaaagaac ggcggcagtg aaatgccgct ttttttgcgc cttattttga
     8701 ggggtattaa ttagtccttt tgatactttt tcatcacgat gataatggaa atcgaaagaa
     8761 taaccagagt tgccattccg caaatgtttg cggctgtgtc cttatttttc tttcccagca
     8821 ttttcattat cctttccttt aatttctgta agcgctataa tgccgcctat atcgcacaga
     8881 gcctcgcggc ttaaagaaat aagacttact ccgtaattcg ccgcaaaggc ttttatttcc
     8941 ttgtatgagg ggtgattttc gatgccgccg caaaaagcga ggcagtcttt cgatataaga
     9001 cccgacgcgc cgccgataaa gccgcagtca aagcctggga gatttacata gccttttttt
     9061 ataaggagga catccacatt gtttttaagg catgccttat atatggattc gtcctcggtt
     9121 attatggcgt tttcggcgac gatagatacg gagcactttg aatagccctg ctgggtttca
     9181 atgattttga ctccaagctc ccgatagtaa tttagaagtt cgggcaaaac ggctgagggt
     9241 ctgcaaatca gcgcggagcc tatgaatgcc gcgttcagcg gtacatcaag ggggtattct
     9301 ctgccgagtt tcgcttttgc gaagctgata ttaaagccaa ggccgctcag agccctttca
     9361 aggctctcct gcccttccga cataaatact tttccgtcga gtgctatgtg gcagagcata
     9421 tcagcgtgca tctttaaagg ctctaaaaca tcgcagcacg gttcggggct gattatctca
     9481 atgccaaaga gttttagttc cctttccgcc tccgggggaa tgccgcccgc caaaaccttt
     9541 tttactttac tctcgggcag attgggcttt aagattggga cttccgaaaa gtatagcata
     9601 gttacgaaat cactttgata gcgtcggcta ttgtttttac agggataatg ttaatctgcc
     9661 ttttaagctc cgcgggcagg gaaacggcgt tgtgatacgg cacgatgcag tgcctgaagc
     9721 ctaaacgcat tgcctcaatt acccgctttt cacagaactg caccgagcgc acctcgcccg
     9781 caagacctat ttctccaaag gcgacaaggt catgatttat aaccacattt ttaaagcccg
     9841 aaacaagaga tagggctacg ggcaaatcgg cggaggtgtc atatattctg atgccgcccg
     9901 ccacatttat gtagctgtcg cattcgccga aattcaggcc tacccttttt tccattacgg
     9961 cgattatcat tgacattcgg ttgtagtcaa agcctgttgc catccttctg ggtgtgccgt
    10021 aggcggattt tgctataagc ccctgaactt cggcaagtat tggccttgtg ccttccataa
    10081 tgcaggcaat gcaggtgccc gacgcgtttt cgggtctgcc tgagagaaag agaagggagg
    10141 gattaaccac atctttaagt ccctcgtttc ccatctcaaa gacgcctatt tcatttgtgg
    10201 agccgtagcg gttctttacg gctctgagga ttctgtatga catattccgc tcgccctcaa
    10261 agagcaagac ggcgtccact atatgctcta aaaccttggg gcccgcaata ttgccgtcct
    10321 tgttaacatg gcccacaaga atgacgggga tattcaaagc ctttgcggtg cgctggataa
    10381 acgaagcgga ttctcttacc tgcacgacgc ttcccggggt tgaggatatt tcgggcaggg
    10441 acatggtctg aattgagtcg actatgacca catccggctt tgcgcttagg atatattcgc
    10501 aaaccgactg ggtatcggtt tcgtttatta tatagagctt gtccgcaaag actttgagcc
    10561 tgtcggctct gagttttatc tggctgacgg actcctcgcc cgcaacataa aggacattga
    10621 atctgctgcc caaaaagtcg caggtttgaa gcaggattgt tgacttgcct atacccggct
    10681 cgccgcacaa aagcacaact gagcccttta caagcccacc gccgagcact ctgtccagct
    10741 cgggtgagcc tgttttgaac ctctgctcgg tgtcggtgcg cacatccatt agattaaccg
    10801 ccttaacggc agatgaagcc ttgggggcgg cggatgcgct ttttacgctc gccactactt
    10861 cctccgtcat ggtgttccac gcgccgcagc ttgggcactg accgtaccat ttggaggatt
    10921 cgtaagcgca ctctgagcaa atataaaccg ttttgctttt tgggctcatt tcaaaatcct
    10981 ttcgatttat ggggaattaa aatattcgtt gattccgcag aagattgcga aggctaattt
    11041 cttttggtaa tcttcgtttt taagctttgc aagctccgga ccgtttgaca taaagccgca
    11101 ctcaatcaaa atagcgggct tttcggcgta gtaaagaaga taaatagact tgcccgactt
    11161 tttaatctcc cgtttgttgt ttggctgcaa aagctctctg atgctttgct ggacattttt
    11221 tgcgaggatt tcgctttcgg ggttattgcc cgaatagaaa acctgagaac cccagtagga
    11281 ggacacgccg aacatatttt ggtgtatgct tacaaaatac gcgttgggcg tttcctctat
    11341 aagcttcatc ctgttaacaa ggtcggtatg ctttttctct cttatggtct ttgcgtcggg
    11401 gtcgctggtg ggggtatcgt ctgtgcgggt gagtatagtc ttgtagcccg ccattcttaa
    11461 tatatcatta agttttaacg ctatttgcag attaaggtcc ttttccacgg ttccgtcatc
    11521 cgcaacggct cccccgtcaa agccgccgtg ccccgcgtca actatgatta taggctctga
    11581 gccgtccgcg cttacgggta tgcccagctt gccgctctga tagtagataa ataccgacag
    11641 ggcgaaaaag gctgccaagg cggcgagaga aaagagtatt ataacaatcc tgcgcttcaa
    11701 aaacatcacc tcaaaaaatt atatgcggaa aagccttcct tatgtttgag gcgcatttga
    11761 agcgcaaatt tttgtatgtt tattctgctg tgttctccgc cgtttcggat tcctccgagg
    11821 tatcctgagc ctcgttctcg ggcgccgatg cttctgtggt ggttacgggc tggggtgcct
    11881 gagtttcggt tctggagggc aaggtcgcgg cggtttcggg cgcgttcttt gcctgaatag
    11941 cgctgatatg atattccccg tttacctcac ggagagtaat gctcatatat ttgtcgggtt
    12001 ccgcgggtat gaaattgccc tgtttgtcct gagtccagct ctcaccgccg atataagcta
    12061 cggtgagaat gacggagctg ctcttctttt ctatgtcggt tactctgggg ctgtagatgg
    12121 gcgacatgcc cgtgaggggg attttataaa ccgcgtccgc cttgtcgtag acaaactcgt
    12181 agccgaagtt tgttatggtc tggtgaacgg gttttacctc ggtgccgaaa agggcggcaa
    12241 agcgcttttc cacatcggcg gcggggataa tcataaagcc gtccctgtac tcatactggt
    12301 cgggggtaag gtcgcctttg agaatagccc ataggctaat gtcaataagg tcggcgctgt
    12361 tggccatcgc aatgtcgtca aagggctctg ggtcgtttgc cacgacgggc accaaaaact
    12421 cgttatacat agcgattttg tcgctgttgt caaagagtga tactatgccg ccgacagcga
    12481 gcttagccgc gttgtaaaag ccgaacaggg agaaaaggac tataagcacg ccgaagaaaa
    12541 acgccacatt cctgcgcatg gcggcgggct cttttttatc ctttgcggag tggaattcct
    12601 gaaccttcgg cttttgctca tagcggctca aatcctgtgc gggctttttc tcatcggctt
    12661 tttccttttc aggctccgct gccgccggtt cttcctttat ttcttccttt ttctcctcta
    12721 cagtaggctt tgcgggctca gtcggctccg cagctttctt ttcggaaaaa taagagggct
    12781 tgccgtcggt cgtttttctt gacagaccga ggctttccag tatttcctca agagggtctt
    12841 tttccgtatt cgcgctattg ttttcagggg tgatgttctt ttctttttcg tccatactta
    12901 gacttcctgt cttatcttat ttgtccctcg cctcttacga catacttgta agaggtaagc
    12961 tcaggcagac ccacggggcc tcttgcgtgg agcttttggg tcgatatgcc gatttccgct
    13021 ccgaaaccga actctccgcc gtcggtaaat cttgtgctgg cgtttacata tacggcggct
    13081 gcgtcaacct cggcacagaa cttttcggcc gcggcaaagc tctcggtcac aatcgcctcg
    13141 ctgtgctttg tgccgtgagt attgatgtgc tttattgcct catctatatc attaacagct
    13201 tttaccgcaa ttatataatc aagatactct gtgtcccaat cttcgtcagt ggcgggcttt
    13261 gcgtcgggta taagtttcag cgcgctctcg tccgctctga tttccacatt cttttctttg
    13321 agcttttcgc agattttggg gagagcggtc tgggcaatat ccttatgaat cagcagcttt
    13381 tcagccgcgt tgcagactga gggtcttgag gtctttgcgt taaagacaat ctctgtcgcc
    13441 atgtcaatat ccgcggattt gtccacatag acatggcagt tgcccgttcc cgtttcgatt
    13501 acggggacag tggagttttc tatgacggat ttaataagac ccgcgccgcc tctggggatg
    13561 aggacatcca catagccgtt aagccgcatt aaagctgtgg cggattctct ggaggtatcc
    13621 tctataaact gaatgcagtc gggggagatg gaggtttgag agagggctga gcgcattgag
    13681 tttactatct ccatgttgga attgaaagcc tctttgccgc ccctgagaat tacggcgttg
    13741 ccagatttaa ggcagagggc ggcggcgtct gcggtgacat tgggtcttgc ctcgtaaata
    13801 accgcaataa cgcccagcgg gactgacact ttagtgattc taatgccgtt tgggcgggtg
    13861 gagccggata ctaccctgcc caccgggtcg ggcaggctga taatctccaa aaccgcgtcg
    13921 gctattgctt taatcctatc tttggtgaga cttagcctgt ctattaaggc gcttgacata
    13981 ccgttttctt tcgccgcgtt tacatccttt tcgttcgcgg caaggatttt gtcggtgtcg
    14041 cgtatcaaag cctcggcaat aagccttaaa gccctgtcct tttcggcaga gccggcgttt
    14101 gctatgctgc ttttagccgc agcggctttt ttgccaattt ccaaaacagc attgctcatt
    14161 gtcagtcacc tcatactttt atttgcatct gaaaagggtt ccgacgctgt gtccgtctat
    14221 aagcttatat atgtcggtcg ggtcgtagcc gttcattaca acggtggtga ttcccgcctc
    14281 ggtagcggtc tttgccgcct gtagcttggt aatcatgccg cccgttcccc tgtttgtgcc
    14341 agctccgctc gcaagggaaa agatttcgtc gtctatttcg tcaacgcagg gaataagcgt
    14401 agcggaggcg tcctccaagg ggttagcgct gaaaagaccg tcgatatcgg agaggattac
    14461 aagggcgtcc gccttgatga gttttgcgac ggtggcggaa agattgtcgt tgtcgccgaa
    14521 aactatttcg tcaacggcga ccgagtcgtt ttcgtttaca atcggaattg ccccgaactc
    14581 aaagagcttt tcaaaggtat ttataagatt gctgcatctt tcggggtcgt ccacatcgct
    14641 cttggtaata aggagctgtc ccacggtgtg accgaactcc gaaaagagct tatcatatat
    14701 gaacatcagc tcgcactggc cgacgcaggc ggctgcctga cggccggggg tgtcggacgg
    14761 cttggattta agacccaatt ttccgacgcc gatgccgatt gcgcccgatg tgacaagtac
    14821 cacttctctg cccgaattct gaaggtccga cagaacctcc gccagcttgc tcattcttct
    14881 gatattggcc tttcccgtat cataagtcag agtggaggtt ccaaccttaa atactattcg
    14941 ctttgcctct tttaatgtgg acataaatct atctttgcct ctctgtatat attaaagcat
    15001 tttgcttttt acgcttttag ctattatatc atcagcgtca tttattgtct ataataagag
    15061 gtttaaactt taagaaatgg gcgtttaaag gatttattaa tttttagtta catgtattaa
    15121 tttagacttt ttgttatttt atgcacaaaa ccaatgttga cgataacagc gtataatgat
    15181 acactatgtt tgttgaattt ttatattttt tgtccttcaa aatagatttc aacggcaaaa
    15241 aaggggttgt gaaaatgaag attaagatag cggctgttat aggcatgggc actaccagtc
    15301 agcgcaactg tgacagcttt aacataaacg gcagagtacc tttaatggcg gaggctgacg
    15361 gcggctacaa ggccgccggc gaaatatcca ctccttttgt gataggagca ttcagcggca
    15421 gcgcagtggg cagcgccttc gcggcggcaa atatgctcac cgaacacgca gaagccatta
    15481 aactcagcga ggaagagtat aaaagcagct ttcttgatta tcttgagcgc ctcagaaacg
    15541 aggttattcg cagagaagag ggcatcatag ccttctcggt ggcgctcctt tacgcataca
    15601 atgacacaat agccgttgca aggctcggca agaacagaat ataccgcttt tcgggcattg
    15661 agctgatgag catccccgag aaccgccaga gcggcggcaa gcagatgagc ggcggcatgg
    15721 accattatga cgacataaag acggaagaca gattccttat cttaggtccc ggcgtggtaa
    15781 acgctcttac cgaaagcga
//
LOCUS       DAONVQ010000033        14777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3058_length_14777_cov_4.555020, whole genome shotgun sequence.
ACCESSION   DAONVQ010000033 DAONVQ010000000
VERSION     DAONVQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14777
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3058_length_14777_cov_4.555020"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLD98_03450"
     CDS             complement(<1..273)
                     /locus_tag="PLD98_03450"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQA05339.1"
                     /translation="MKIYLASASPRRREILNLAGIRHSVVLPSVSEDLEDGVKPCNAV
                     VMLSERKAEAAFALIDERDDDFAVIAADTVVALGDKIFGKPRNGKDA"
     gene            1496..2227
                     /locus_tag="PLD98_03455"
     CDS             1496..2227
                     /locus_tag="PLD98_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQA05340.1"
                     /translation="MSLKWHHLLFPHRCLFCGSPVTASERICEMCMEALPIIGAPVCF
                     FCGISKADCACREKRHFYDAIIAPFYYEGAVAKGIRLMKFAGRTENAEYFGEAISVCA
                     SHYYSEIAFDCVCFVPESEDVLKERGYNQSRLLAESVSRHLNVPCGELLKKLYTTKPQ
                     RTLSAAERQGNVLGVFETNPEEITAGKKILLIDDIKTTGATLDECAKSLLIAGADRVF
                     CAVAAVSPMRASQNEETAEETLTAK"
     gene            complement(2295..3998)
                     /locus_tag="PLD98_03460"
     CDS             complement(2295..3998)
                     /locus_tag="PLD98_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQA05341.1"
                     /translation="MSIPRSEHPNPQFMRESWVCLNGEWDFEIDQSVSGKARKLYAKD
                     APFSQKIIVPFCPESKLSGIEHKDFLHCVWYRRSFFLTKEQTEKRVVIHFGAVDYEAF
                     VYVNEKPVGSHKGGYISFSFDITNYVVEGENIITVCAVDDVSDPMVPRGKQSEEYYSH
                     GCDYTRTTGIWQTVWLEFTEKSYIDSVKYYPDSENGILTIEAKLVGKGIFLAEAYYEG
                     RPVGKAEAQCNHGCAKVQIELSEKHLWEVGCGRLYDLKLSFCGDEVTSYFGLRNVCLD
                     GYKFRINGKSVFQRLVLDQGFYPDGIYTAPTDEALLNDILISKAVGFNGARLHEKIFE
                     PRFLYHCDREGYIVWGEYPNWGLDHSRPEAVFSILPEWIEELRRDFNHPSIIGWCPFN
                     ETWDVKGRKQCDEILSLVYHTTKAIDPTRPCIDTSGNYHVVTDIFDLHDYDQSPESYK
                     KKFGSFGKDQPPHDNHAKRQTYRGEPLFISEYGGIRWSDDANSWGYGDTPKSEEEFIA
                     RYKGLTDVLLDNENIFGFCYTQLYDIEQEQNGLYTYDRKPKFDPAIFKAINERKAAIE
                     D"
     gene            4258..5172
                     /gene="argF"
                     /locus_tag="PLD98_03465"
     CDS             4258..5172
                     /gene="argF"
                     /locus_tag="PLD98_03465"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQA05342.1"
                     /translation="MKHLLKLADLTTEEIISILNLADRLKKERKNGINHTYLAGKTLG
                     MIFQKASTRTRVSFEAGMYQLGGNALFLSDRDLQIGRGESMEDTARVLSRYIDAIMIR
                     TFKQEDVENLARYSSIPVINGLTDYAHPCQVLADLMTIREYKKNLEGRTLCYIGDGNN
                     MANSLIVGGIKTGMKVRIACPSSNKPDAKIMEWAEKNGDFICTEDVFLAAKDADVLYT
                     DVWASMGQEHEIEERRRIFKNYQINDEVLKVAKSDAMVQHCLPAHKGEEITTEVFDKH
                     ADEIFDEAENRLHVQKAVLLKLLMPEIN"
     gene            5322..6251
                     /locus_tag="PLD98_03470"
     CDS             5322..6251
                     /locus_tag="PLD98_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05343.1"
                     /translation="MTKIKAIVSILLVLSLMFGIAACGKDKGGDETTVEETTAEETAG
                     ETSANDETSAAETSEPASGDETSAAPSETQAQTEAPSQNPGNNSKFPVGTDKAAIVEF
                     YNKAANAVKAYKGTLKVTRKSGAYTKIEKFDVPLVGGSVRKLAEDKLPNDWDKAQPKT
                     LTFNNGVAKDGDKDRTLAEVLPPEKAEYMSKLTPAGVKSAECKLEGSDAKVTITLVEE
                     SSTSLDFVPPHHSSCLDTLKLSSADLDPFTIKNAKVTYKGATITAVINEAGQLLSLQV
                     REPAYVEGNVGYSALTFPAAISGGYNADYTFVW"
     gene            6611..7555
                     /locus_tag="PLD98_03475"
     CDS             6611..7555
                     /locus_tag="PLD98_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05344.1"
                     /translation="MKKNAFRLVSLLLVIAMVFAIGACGKKGDETTTAEETTVEETQD
                     AGATEATGETAATSETAAETEAQTEGQTEAQTQAPSQPASEVPQGKEAILAAYTKVMN
                     KAKQDKPAYKKIEFQKIPDDSQNRKVRKGENTIGRLLGVVDSLGVMTPEDKAKVEELQ
                     KGNDMKWFPIHKNSKGCLLTDVSAIKEAKAEKLANGNIRLEITLVDEKNSQPAAENAA
                     TSPSKIGAMFSPLAKADIDNTLNSGVVKAVVRDIKYDLTFHDCKAVLEYNPANDQIVK
                     LEQYMVIRIDAQGKVLLLGDFDLTQELYNTMKTYDFVY"
     gene            complement(7603..8352)
                     /locus_tag="PLD98_03480"
     CDS             complement(7603..8352)
                     /locus_tag="PLD98_03480"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HQA05345.1"
                     /translation="MIKILCIGNSFSEDAMKYLHAIAKCGGMEVKTVNLYIGGCPLIK
                     HWKNAKSFRKAYDYQLNGERTGKKISMRKALKSETWDYISFQQASYDSGVEESYYPYL
                     TLLSEYAKKKSPGAVQLIHKTWAYEIDSIHDGFAKYERNQKLMFDSLSKAYKKAAEAI
                     NAEIVPVGDVIQALRATKEFDYEKGGLSLCRDGFHLDYIYGRYAAAATWYEKVLKGNI
                     LENSFIPEYNGIRADEALIEAIKRTVHEICS"
     gene            complement(8345..9436)
                     /locus_tag="PLD98_03485"
     CDS             complement(8345..9436)
                     /locus_tag="PLD98_03485"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQA05346.1"
                     /translation="MDEGKIQDIDKNLKVETNLKLDDICFYDARREPFRLYGLYNAKT
                     EAEFKRLPDDVAQSTNQGVAALYKNTAGGRVRFSTDSSYVAVKAVMPGIGKLPHMPLS
                     GSAGFDLYINDGGKDVYFKTFMPPYDIKDGYESVIHFEDRRARNITIHFPLYSCVSSL
                     YIGLQEDANVGEGAKYKYEKPVLYYGSSITQGGCASRPGNCYQNIISAKLGCDHINLG
                     FSGSARGEDIIADYIASLDYSIFVCDYDHNAPSVEHLEKTHEKLFLKVREAIPEVPIV
                     LVSKPDFDSNPAEIAKRRDVIYRTYMNALRRGDKNVYFIDGERLFKDENRDACTVDGC
                     HPNDAGFVRMAEVIGFTVDKILRKGKSHD"
     gene            complement(9586..9670)
                     /locus_tag="PLD98_03490"
     tRNA            complement(9586..9670)
                     /locus_tag="PLD98_03490"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9634..9636),aa:Tyr,seq:gta)
     gene            complement(9675..9750)
                     /locus_tag="PLD98_03495"
     tRNA            complement(9675..9750)
                     /locus_tag="PLD98_03495"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9715..9717),aa:Thr,seq:tgt)
     gene            complement(9897..11360)
                     /locus_tag="PLD98_03500"
     CDS             complement(9897..11360)
                     /locus_tag="PLD98_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05347.1"
                     /translation="MKPISLATKLLTGYIKTSGKIGSVFKSVPQIASADFDYMKPSDN
                     TTFTVGFGKAPMMPEDITKKKYYVAGYDINKPATGILDPLYAHAIWLDDNSGRGGVVF
                     VSLDAVGLLKTDADIIKESLGDFLAESGCRSINIISTHSHASIDTMGIWGPLPRTGRS
                     ADYMTTVKEAVITAVKAAYADRRTGKLYLGRVEVPDMQKDIRTPVVFSKILTRLRFVP
                     DDKSREIHFINFASHPETLTSENSLISADFPAYLRERIKEKTGADTIYFSGAIGGMIT
                     MSSGGKTMPEKIEGTKNLGRRLGDYALSITDEVELKANINLIKQEFFVEAENIVLMTA
                     AQLSILHTNRYHSKEASLGYLLRTEVSYFEIGELKMLLLPGETFPEVVYGGYLSKEES
                     ATGLGPEANPDPLIEIVGDPNLLVFNLANDEIGYILPPNDFLLSEDAPYLERAKDRHG
                     RNHYEETNSVGPKTAVKIAEVVREIMETVNRTKADNS"
     gene            11518..12354
                     /locus_tag="PLD98_03505"
     CDS             11518..12354
                     /locus_tag="PLD98_03505"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQA05348.1"
                     /translation="MNKRLFEKEREVKSLLQGFSANRLVLCLDSTESTNIIAREFLTK
                     GKAFDLEYKKDAPPLIIAAEQTMGEGRLGRCFESKRDMGLYMTAVYPLTGHEQYLSLL
                     TLLAGLSSVKAAEKTVNSKGQKINLTLKWPNDIYFDGKKLGGIIVKMINYPNIAAIVG
                     IGINLNQSGEDFPSEIADRAVSFKMAGIDISSSELAAEIINTLDKAVYEDKSLTSPAP
                     EILNELNNKSFTLGKRVSFTIDGKELVGTAEEILKNGALAVKTDDGRLYEVISGEAGF
                     AV"
     gene            12997..14082
                     /locus_tag="PLD98_03510"
     CDS             12997..14082
                     /locus_tag="PLD98_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQA05349.1"
                     /translation="MANKLFQSVIHQMKDVISRSVGVIDASGIVISSSDLGAIGEVRA
                     QAAEAVFGSEQGLSFEGFTYKPFGSQGRPEYAVFVEGEDDFAATQCKVLAISLSSLKQ
                     FYDEKYDRGNFIKNVMLDNILPGDIYIKSRELHFDNDTTHAVLLIRLTEPTELSVIDI
                     IQPLFPDREKDFVISINETDIAVVKEVPQSIESKDLEKLARSVSDALAAELYTKTLVG
                     IGTAVTGIKELARSFKEAQVALEVGKVFDTEKTIVSYENLGIARLIYQLPTTLCEMFL
                     REVFKRGSIDHLDSETLFTIQKFFENNLNVSETSRKLFVHRNTLVYRLEKIRKLTGLD
                     LREFDDAIVFKVALMVKKYLDVTPSKF"
     gene            14273..>14777
                     /locus_tag="PLD98_03515"
     CDS             14273..>14777
                     /locus_tag="PLD98_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA05350.1"
                     /translation="MIEFRNLTKIYPNGTKALKDVNIKIEKGEFVFIVGASGAGKSTL
                     LKIMMREEVATSGSVIINNYNLNAIKKKDIPKFRRTMGIVFQDFRLIPNLSAYDNVAF
                     AMRVIGAKEREVRKRVTHTLSLVGLSSKAKCLPAQLSGGEQQRVALARALVNNADLII
                     ADEPTGNI"
BASE COUNT         4110 a         3386 c         3324 g         3957 t
ORIGIN      
        1 ggcgtccttg ccgttcctgg gctttccgaa tattttgtca ccgagagcca caaccgtgtc
       61 ggcggcgatt acagcaaaat catcatctct ttcgtctata agagcgaacg ccgcctcagc
      121 ctttctttca gagagcatta ccactgcgtt gcagggtttt acgccatcct ccaaatcctc
      181 cgaaaccgaa ggcagcacta cggaatgtct tatgcccgca aggtttaaaa tctcccttct
      241 gcggggcgaa gcggatgcaa gatatatctt cattttcgtc tccgttattg ctatagattt
      301 aaacgattac aactaattta acacaaccta accttcaagt caattttgcc catttaaact
      361 ttggctatgg caataaaatt aatatccgga aaacgaatat tattgtaaaa atcgtacaat
      421 aagtatttat ttcaaattca aacttataac aaatagttgc aaaaatgttg ttctatatta
      481 ttggtgaaca gaagagtagt cccgaatatt aaatgacaaa tttttacggc agctcaaaga
      541 tatagaagaa aatattacaa cattttgtta ttcatgttaa tttttattcg cagctttact
      601 tttattgaat tgtttgtcct gttgtggtaa tatatgttca ataatataag tggtaatgtg
      661 tttatcggga ctgagtgatt tacttatatg tagcagagga tggtagtatg tgagtatgga
      721 gaatttcaca aagttggtcg aactgatagt atatattatt aactttctga gagagctgtt
      781 taacagaaac aagacctctt agcggttaat aatagcgatt tatttcggat atttgcatct
      841 gtcaaagatg taaaattata tctcattctt tttggcttag catgaaaaac gaaagtgagg
      901 tgcagcagaa tgttaggagc tttccaggac ggctttgctt ggcttgcaga gatcatcaat
      961 tacttcaaag agcttttaaa gaagcttggc gttgagttct aattaaagcg gctatcaaag
     1021 ttgcgcaact tttgcaaaat accttttagt aaatgtttaa aggagggatc actttgaccg
     1081 ctgctgaact tatcgaagtc gtaagaagac atgccgccat gttcaatgat ctggtagctt
     1141 tcatagtcag attctatgag cgtgtcaaag ctaacgctga atgctgaata agctttaacc
     1201 gagtttccta aggattaatc cttggtttgt tggtggctat tttggttttg aaagtgaggt
     1261 gtaacagaat gacaggagaa gaaatagtag cactcatcaa agagttcttc gatatgattc
     1321 ttaacttcct tatcgcagtt ggcatcatta aggccgatga tgaggagtaa tcttctgatt
     1381 ggtttattgc tgtgttgtta aagaaagaga tcggatttct gatctctttc tttattttgt
     1441 cttttctttt ccgcgtgata taatggatta aaattgtttc gtaggaaggt gcaaaatgag
     1501 tttaaagtgg caccatcttc tgtttccgca cagatgcctt ttctgcgggt cgcctgtcac
     1561 agcgagtgaa aggatttgcg aaatgtgcat ggaagcgctg cccattatag gcgcgcccgt
     1621 ctgctttttc tgcggcattt caaaggcgga ctgcgcctgc cgagagaaaa ggcattttta
     1681 tgacgccata atagccccgt tttactacga gggcgctgtg gccaaaggaa taaggctcat
     1741 gaaattcgct ggcaggacgg aaaacgccga gtatttcgga gaagcgattt ccgtttgcgc
     1801 aagccactat tacagcgaaa tcgcctttga ctgcgtctgc tttgtccccg aatctgagga
     1861 tgtgctaaag gaaaggggct acaaccagag caggctgctg gctgagagcg tgtcaaggca
     1921 tttgaatgtc ccgtgcggag agcttttaaa gaagctctat acaacaaagc ctcaaaggac
     1981 tttgagcgcg gctgaaaggc agggcaatgt tctcggcgtg tttgagacta atccggaaga
     2041 aataacagcg ggcaaaaaaa tactcctgat tgacgacata aaaaccacag gagctaccct
     2101 tgacgagtgc gcaaaaagcc ttttaatcgc gggagcggac agggtattct gcgccgtcgc
     2161 cgccgtcagc cccatgagag cgagccaaaa tgaagaaaca gccgaagaaa ctctaaccgc
     2221 aaaataaaaa ccctgagcat ccgttgtaca gctcagggtt tcattttttt tgctttggtt
     2281 aatcgagaaa atgcttaatc ctcaatagcg gcctttctct cgtttatcgc cttaaatatt
     2341 gcgggatcga atttcggctt tctgtcatat gtgtaaagtc cgttctgctc ctgctcaatg
     2401 tcataaagct gagtgtagca gaatccgaat atattttcgt tgtccaaaag cacatcggta
     2461 agccccttgt agcgggcgat gaactcctcc tcagatttcg gagtgtcgcc gtagccccag
     2521 gagtttgcgt catcgctcca acgaatgccg ccgtactcac tgatgaacag cggctcgccc
     2581 ctataagtct gcctttttgc gtgattgtca tggggcggct gatccttgcc aaaggaaccg
     2641 aacttctttt tgtagctctc aggcgactgg tcatagtcgt gcaggtcgaa tatatccgtc
     2701 acaacatgat agttgccgct ggtatcgatg caggggcggg tggggtcaat agcctttgtg
     2761 gtgtggtaga caaggcttaa aatctcatcg cactgctttc tgccctttac atcccatgtt
     2821 tcgttaaagg ggcaccagcc gatgattgac gggtggttaa agtcgcggcg cagctcctca
     2881 atccattcgg gaaggatgga gaaaacagcc tccgggcggg aatggtcaag tccccagttg
     2941 gggtactcac cccagactat atagccctcc ctgtcgcagt gatagaggaa tctgggctca
     3001 aaaatctttt cgtgaagtct cgctccgtta aagcccacag ccttggagat aagtatatcg
     3061 tttaaaagag cctcatcggt gggagcggtg tagattccgt cgggataaaa gccctggtcc
     3121 aaaacaagac gctggaatac cgacttgccg tttatgcgga acttatatcc gtcaaggcag
     3181 acatttctaa ggccaaaata gcttgtaacc tcatcgccgc agaaacttaa ctttaagtca
     3241 tagagcctgc cgcagcccac ttcccaaagg tgcttttcac taagctctat ctgaacctta
     3301 gcgcagccgt gattgcactg ggcctctgcc ttgcccacgg gtctgccctc ataataagcc
     3361 tcagctaaga aaattccctt gcccacgagc tttgcctcaa ttgtgagaat gccgttctcg
     3421 ctgtctgggt agtatttaac gctgtcaata tagcttttct ccgtaaactc aagccatacc
     3481 gtctgccaga ttccggttgt ccttgtatag tcgcagccgt ggctgtagta ttcctcgctc
     3541 tgctttcctc tgggaaccat cgggtcgcta acatcgtcca cggcgcatac ggtgataata
     3601 ttttctcctt cgacaacata atttgtaata tcaaaggaaa aagagatgta tccgcccttg
     3661 tggctgccga ccggcttttc gtttacataa acgaatgcct catagtcgac ggcgccgaag
     3721 tgaatgacaa cccttttctc agtctgctct ttagtaagaa agaagctgcg cctgtaccaa
     3781 acacaatgga ggaaatcttt gtgctcaatt cccgacagct tgctttccgg gcagaaggga
     3841 acaataatct tttggctgaa aggagcgtcc ttcgcataaa gctttcttgc ctttccgctg
     3901 acgctctggt ctatttcaaa atcccattcg ccgttaaggc aaacccaaga ctctctcata
     3961 aactgcgggt tcggatgctc gcttctcggt atgctcatac ttattctcct ttgttttaac
     4021 acttttacat attctaacca atcgggactt ctaattcaag cacaaaattt tcttatattt
     4081 atgcacaaat gcaaggttat atgagggtgt tggcttaata aaactataaa attttgaaaa
     4141 attctttgta tatttacaca tttttgttga cataacggaa ttaagggatt aagataattt
     4201 atataaaatg gaaatttgta ttacgataga acaggatata aaaaggggac ttataaaatg
     4261 aaacatttgc ttaaacttgc cgatttaaca accgaagaaa tcatatcaat cctaaacctt
     4321 gccgacaggc tcaaaaagga gcgcaagaac ggcataaatc atacctacct tgcgggcaaa
     4381 accctgggca tgatattcca aaaagcctca acccgcacaa gggtatcctt tgaggcgggc
     4441 atgtatcagc tgggcggcaa cgctctgttc ctcagcgaca gggatttgca aatcggtcgt
     4501 ggagagtcca tggaggacac cgcaagagtc ctttcaagat atattgacgc gattatgata
     4561 aggacattca agcaggagga tgtggagaat cttgcccgct acagcagcat acccgtaatc
     4621 aacggtctta cggactacgc ccacccctgc caggttctcg ccgaccttat gaccattagg
     4681 gaatacaaga aaaaccttga gggcaggaca ctctgctata tcggcgacgg caacaatatg
     4741 gcgaactcac tcatagtggg cggaatcaaa acgggcatga aggtacgcat agcctgcccg
     4801 agttccaata agccggacgc gaaaataatg gagtgggcag aaaagaacgg cgactttata
     4861 tgcaccgagg atgttttcct tgccgcaaag gacgcggatg tgctctacac cgatgtatgg
     4921 gcatcaatgg gtcaggagca tgaaatcgag gagcgcagaa ggattttcaa aaactatcag
     4981 ataaacgacg aggtattaaa ggtcgcaaag agcgacgcaa tggttcagca ctgcctgccc
     5041 gcgcacaagg gcgaggaaat cacaaccgaa gtctttgaca agcacgcaga cgagattttt
     5101 gatgaggcgg aaaacaggct ccatgtccaa aaggcggtac ttctcaaact tttaatgccc
     5161 gaaatcaact aaatattgcc gtcaaaatgt tttgaacata catttaacaa attgttatcc
     5221 atttatttat tcttactata ttgacataat gatgtcttta tagtacatta tggttgtgta
     5281 ttctgcacat caatatccca tttttgaaag gagtaaatcc cgtgacaaag attaaagcga
     5341 tagtatcaat tcttcttgta ctatccttaa tgttcggcat tgctgcctgc ggtaaggata
     5401 agggcggcga cgaaaccaca gtggaagaaa caacagcaga agaaacagca ggcgagacca
     5461 gtgcaaacga tgagacatcg gcagctgaga cctcagaacc tgcttcaggc gacgagacat
     5521 ctgctgcccc cagtgagact caggcccaga cagaggctcc ctcacagaat cccggaaaca
     5581 actccaaatt ccccgttggc actgacaagg ctgctatcgt agagttctac aacaaggcag
     5641 ctaacgctgt taaggcttac aagggaaccc tcaaggtcac aagaaagtcc ggcgcttaca
     5701 caaagattga gaagttcgat gttccgctgg taggcggctc tgtaagaaag cttgcagaag
     5761 ataagctgcc gaatgactgg gataaggctc agcccaagac ccttacattc aacaacggcg
     5821 ttgccaagga tggcgacaaa gacagaactc ttgctgaagt tctgcctccc gaaaaggctg
     5881 aatacatgag caagctcaca cctgcaggcg ttaagtccgc agagtgcaag ctggaaggca
     5941 gcgatgccaa ggttacaatt actctcgtag aagagtccag caccagcctt gactttgtac
     6001 cgcctcacca cagctcatgc cttgacacac ttaagctcag ctccgctgac cttgacccgt
     6061 tcaccattaa gaacgcaaag gttacataca agggcgctac catcacagct gttattaacg
     6121 aagctggtca gctcctttca ctccaggtca gagagcccgc ttatgttgaa ggcaatgtag
     6181 gctacagcgc attgacattc cccgcagcta tcagcggcgg ttacaatgct gactacacct
     6241 ttgtttggta attaacatta agatttcagg gtaaggcaat gccttgccct gattttttta
     6301 tcttaaaagt atcctgcctt attagccaaa gccgcatatt ttcttttcgc ctttaataag
     6361 atttatcgga tacaaaagtc aaagcatcat tttcacttgt cagatttagt cgaacaatat
     6421 caaaattttc cttatcgaaa aatatcggtt tgttcgttaa tttgtagcat ttgtcacaaa
     6481 ggcttattaa ttaaaacctt ttttcgtata attagcataa ggatattgac tttataagat
     6541 tcttgttgta aattaataaa ggcgattatc gctccaacga ctgcgaatat ttgaaagggg
     6601 tacataactg atgaagaaaa acgctttcag actcgtttct cttttgcttg tgatagcaat
     6661 ggtatttgct atcggcgcat gtggcaaaaa aggcgatgaa acgactactg ctgaagaaac
     6721 cacagtagaa gaaacccagg atgcaggcgc aacagaagct actggcgaaa ccgcagctac
     6781 cagcgagaca gctgctgaaa cagaagctca gacagaaggt cagacagagg ctcagacaca
     6841 ggctccctct cagcccgctt ctgaggttcc ccagggtaaa gaggctatcc ttgcggctta
     6901 tacaaaggtt atgaacaagg caaagcagga taagcccgct tacaagaaga ttgagttcca
     6961 gaagattccc gatgactctc agaacagaaa agtcagaaag ggcgaaaaca caatcggcag
     7021 acttcttggt gttgttgact ccctcggcgt tatgacaccc gaagacaagg caaaggttga
     7081 agagctgcag aagggcaacg acatgaagtg gttcccgatt cataagaact ccaagggctg
     7141 ccttctgacc gatgtatccg ctattaagga agctaaggca gagaagctgg caaacggcaa
     7201 catcaggctg gagattactc ttgttgacga gaagaactca cagcccgccg ctgaaaacgc
     7261 agcaacttca cccagcaaaa tcggcgcaat gttttcacct cttgcaaagg ctgatatcga
     7321 caacaccctt aacagcggcg ttgttaaggc tgttgtaaga gacatcaagt acgaccttac
     7381 atttcatgac tgcaaggcag tgcttgaata taatcccgca aatgaccaga ttgtaaagct
     7441 tgagcagtac atggttatca gaatcgatgc tcagggtaag gttttactcc tcggcgactt
     7501 cgatttaacc caggagcttt acaatacaat gaagacctac gatttcgttt attaatctcc
     7561 ggcaaaacaa taaagggaag ctttaagctt ccctttttct ttttatgagc atatttcgtg
     7621 cacagttctt tttattgctt caatcaaagc ttcatcagcc cgtatgccat tatattcggg
     7681 aataaagctg ttttccagaa tattgccctt cagcaccttc tcataccagg ttgccgcggc
     7741 ggcgtatctg ccgtagatgt aatcaagatg aaagccgtca cggcaaagag agagcccgcc
     7801 tttttcgtag tcaaactcct ttgttgccct cagagcctga ataacatcgc ccacgggaac
     7861 tatctcggcg tttattgcct cagccgcctt cttatacgcc ttgctcagag aatcaaacat
     7921 tagcttctga tttctctcat actttgcgaa gccgtcatgg atgctgtcaa tctcataagc
     7981 ccaggtcttg tgaatgagct gaacagcccc gggcgatttc tttttagcgt actcagacag
     8041 caaagtaaga taagggtaat aggattcttc cacaccgctg tcgtaacttg cctgctggaa
     8101 gctgatatag tcccaggtct cgctcttaag agcctttctc atggagattt tcttgcccgt
     8161 tctttcgccg ttaagctgat agtcataagc ttttctgaag ctttttgcgt tcttccagtg
     8221 tttaatgagc gggcagccgc ctatataaag gtttacggtt ttaacctcca tcccgccgca
     8281 ctttgcgata gcgtgcaggt atttcatagc gtcctcggaa aagctgtttc cgatgcagag
     8341 aatcttaatc atgtgacttg ccctttctta aaattttatc caccgtaaaa ccaataacct
     8401 cagccatgcg gacaaagccc gcgtcgttcg ggtggcagcc gtcaacggtg catgcgtcgc
     8461 ggttttcgtc cttaaacagc ctctcgccgt caataaagta cacattttta tcgccgcggc
     8521 gcagagcatt catgtatgtc ctgtaaatca catctcggcg ctttgcgatt tctgcgggat
     8581 tgctgtcaaa atcgggcttt gaaacaagga caatcggaac ctcaggtatt gcctctcgaa
     8641 ccttcaaaaa aagcttttcg tgggtttttt caagatgctc caccgacggg gcgttatggt
     8701 catagtcgca aacaaaaatg ctgtaatcaa ggctagctat atagtctgct attatatcct
     8761 cgccccttgc gctgcccgaa aagccgaggt ttatgtggtc gcagccgagc tttgcggaaa
     8821 tgatgttctg atagcagttg cccgggcggg acgcgcagcc cccctgggta atggaggagc
     8881 cgtaataaag aacgggcttt tcatatttat acttagcccc ttctcccaca ttagcgtcct
     8941 cttgcagacc tatatacaat gaacttacgc agctgtagag cgggaaatga attgtgatgt
     9001 tgcgagcgcg cctatcttca aaatgaatta ccgactcata gccgtccttt atgtcatatg
     9061 gaggcataaa ggttttaaag tagacatcct ttccgccatc gtttatataa aggtcaaagc
     9121 ccgcagagcc tgaaaggggc atatgcggca gctttccgat acccggcata accgccttaa
     9181 cagcgacata agaggagtcc gttgaaaagc gaaccctgcc gcccgctgta ttcttgtaaa
     9241 gagcggcaac gccctgattt gtgctttgag ccacatcatc gggcaagcgc ttaaactcag
     9301 cctcagtctt ggcgttgtaa agcccgtaaa gcctaaaagg ctcccgcctt gcatcataaa
     9361 agcatatatc gtccagttta agatttgttt ccacctttaa attcttgtct atatcctgaa
     9421 tctttccctc gtccatacat ctttctcctt tgaaaacttc aactaaataa tataagaatt
     9481 caccctctaa agcaaacatt ttctccgaaa cttactggat ttagtatgag cctaaacacg
     9541 cttgtgatat aaaaaaaccc gtcagtgtaa cactgacggg ttttgtggtg ggagagggcg
     9601 gattcgaacc accgaagcca ctggcaacag atttacagtc tgcccccttt gaccgctcgg
     9661 gaactctccc atgatggagc tggtggacgg actcgaaccc ccgacctgct gattacaaat
     9721 cagctgctct accaactgag ctacaccagc aacgcattcg tatcgtgcgt gccaaagcaa
     9781 gagataatat atcacaagca attatttaag tcaagcactt tttatacttt tttaagaatt
     9841 ttgatatttt aaaagggaga aggaacgccg cccatctccc tttacgcttt atttgtctat
     9901 gaattatcgg cctttgttct gtttactgtt tccattattt ctctaactac ttcggctatt
     9961 ttgaccgccg ttttgggacc taccgagtta gtctcctcat agtgatttcg gccgtgcctg
    10021 tcttttgccc tttctagata gggagcgtcc tcgctcaaga ggaaatcatt cggcgggagg
    10081 atgtagccga tttcgtcgtt tgcaaggtta aataccagca gattcgggtc gccgacaatt
    10141 tcgataagcg ggtcggggtt tgcctcggga ccgagacccg ttgccgactc ctctttgcta
    10201 agataaccgc cgtaaacaac ctcggggaag gtctcgccgg gcaagagcag cattttcagc
    10261 tcgccgattt caaaatacga aacctcggtt ctaagcagat atccaaggct tgcctctttg
    10321 gaatgatacc tgttcgtgtg cagaatagaa agctgagcgg cggtcataag aactatgttc
    10381 tcagcctcta caaagaactc ctgctttata aggttgatat tagccttcag ctcaacctca
    10441 tcggtaatgg agagagcata gtcgcccagt ctccttccaa ggttcttggt gccctcgatt
    10501 ttctcgggca ttgtcttgcc gccggagctc atggtaatca tgccgccgat tgcgcccgag
    10561 aaataaatag tatcggcgcc cgttttctcc ttgattctct ccctaagata agcggggaag
    10621 tcagcgctga taagagagtt ttcggatgtc agcgtttcgg gatgggatgc aaagtttata
    10681 aagtgaattt cccttgactt gtcatcgggc acgaacctga gccttgtaag aatctttgag
    10741 aaaacaacgg gggttcttat atccttctgc atatcgggaa cttcaactct gccgagatac
    10801 agcttgcccg tgcgtctgtc ggcgtacgct gccttaacag cggttattac agcctcttta
    10861 acggttgtca tgtaatcggc gctcctgccc gttctcggca gcgggcccca aattcccata
    10921 gtatcaattg aggcgtggga gtgggtgctt ataatattta tgctgcggca gccgctctcg
    10981 gccagaaaat ctccgaggct ttccttaatg atgtcagcat cggtctttaa aagtcctacg
    11041 gcgtcgaggg acacaaaaac aacgccgcct ctgcccgaat tgtcgtcaag ccaaattgca
    11101 tgggcataga gggggtccag aattccagtg gcgggcttgt ttatgtcata gcctgcgaca
    11161 tagtatttct ttttggttat gtcctcgggc atcattggag ccttgccgaa acccactgta
    11221 aaggtggtgt tgtcgctcgg cttcatatag tcaaaatcgg cgcttgcgat ttggggtaca
    11281 cttttaaata ctgagcctat tttcccgctc gtttttatgt agcctgtcag cagtttggtt
    11341 gccaaagata ttggtttcat atcctcagtc ctctctgaat actttagtat tacagtagca
    11401 caaattgcca tttgccgcaa caattatgag tcttctcata attctttttt tgccttttgt
    11461 attgcacaaa aaactacggc gggctaaaat gatgcaaaga ctaaaagggc tgattggatg
    11521 aataaaaggc tttttgaaaa ggaaagggaa gtaaagtcac tgcttcaagg cttttcggca
    11581 aacaggctcg ttttgtgcct tgacagcaca gagagcacca acataattgc aagagaattc
    11641 ctgacaaaag gcaaagcctt tgatttggaa tataaaaaag acgccccgcc cctgataatt
    11701 gccgctgagc agacaatggg ggagggcaga ctcggcagat gctttgaaag caaacgggat
    11761 atgggccttt acatgacggc ggtttacccc ctcaccggcc acgaacaata tctctcactt
    11821 cttactctct tagcgggtct gagcagcgta aaagccgcag agaaaacggt aaatagcaag
    11881 ggacagaaaa ttaatcttac tctcaaatgg cccaacgata tttactttga cggcaaaaag
    11941 ctgggcggca taattgtaaa aatgattaac tacccgaata tagccgccat agtcggcata
    12001 ggcataaatc tcaatcagtc cggagaagat ttcccaagtg aaatcgccga cagagcggtg
    12061 tcttttaaaa tggcgggaat cgatatttca tcctcagagc tcgccgcaga aataataaat
    12121 accctcgaca aagcggttta tgaggacaaa tccctcacaa gccccgcccc ggagattctt
    12181 aatgagctta acaataaatc cttcacctta ggcaaaagag tgagtttcac cattgacggc
    12241 aaagagcttg tcggcaccgc ggaagaaatc ttgaaaaacg gtgccttagc cgttaaaacc
    12301 gatgacggca ggctatacga agtaatatcc ggcgaagcgg gcttcgcggt ataaacttgt
    12361 cacaataaaa ggttcacccc gggaaaagag cagaggcttg actcaaaacg cactttcccc
    12421 ggggactatt ttatgcgaag cctgtagcca agggcaattc tgaggacccc tgcggcaagt
    12481 ctcgaggtca cccttgacca agcctcgctt atactgaatt gcagcacggt gctaaggcga
    12541 atttagcggt tgcaatcagt attaacggtg ccgcagtaat agtttatgct tgtcaatgcg
    12601 tttgtgttat aaaattttag cccgcccaat ccttctcaaa acaaacaaaa atttttgtcg
    12661 aaattaggtg ttttgaataa aaaccgcctc ttacggtttt actgtcggaa aaactgtaat
    12721 ttgtcatttg gtgataatga acaaagttat tttatcaaaa tccctgcgca aaatattgta
    12781 gaaatctcat aaaaaatttt cttttagagc aaatttttaa atatttatta aaataaagca
    12841 tttatttttt caataatact tgaaaagtcc gttgtaattt tgtagaatgc ttaaatagaa
    12901 taggtaaaaa tgcataaatt aattaacaaa tgaagttttg tgttattttg cattgtgcag
    12961 aaggttttat aaaataaaga gtagggcggt agatttatgg caaataagct gtttcaatct
    13021 gtcattcatc agatgaagga tgttatcagc agatcggtag gagtaataga cgcatcggga
    13081 atagtaatct caagctcgga tttgggagct atcggtgaag tcagagcgca ggctgcggaa
    13141 gcggttttcg gctcagagca gggtctttct ttcgagggct ttacctacaa gcccttcggc
    13201 agtcagggcc gccccgagta cgctgtcttt gttgagggtg aggacgactt tgccgcaacc
    13261 cagtgcaagg tgctcgctat ttccctcagc agcctcaagc agttctatga cgaaaagtac
    13321 gacagaggca actttatcaa aaatgtaatg ctcgacaata tcctccccgg cgatatatat
    13381 attaagtccc gcgagctgca ttttgataac gacacaaccc acgcggttct tcttatcagg
    13441 cttacagagc ctacagagct ttcggttatc gacattatcc agcccctttt ccccgaccgt
    13501 gaaaaggact ttgtcatcag cataaacgaa accgatatag ctgttgttaa agaggttccc
    13561 cagtccatcg aatccaagga ccttgaaaag ctggctcgct cagtatccga cgcccttgcc
    13621 gcggagcttt acaccaagac actcgtcggc atcggcaccg cagtaaccgg cattaaggag
    13681 cttgcccgtt catttaagga ggctcaggta gcccttgagg tcggcaaggt ctttgatacc
    13741 gaaaagacaa tagtcagcta cgaaaacctc ggtatagcaa ggcttattta tcagcttccc
    13801 acaacccttt gcgagatgtt cctcagagag gttttcaagc gcggctccat tgaccacctt
    13861 gattcggaaa ccctctttac aattcaaaag ttcttcgaga acaacctcaa tgtttccgag
    13921 acatcaagaa agctctttgt tcacaggaac accctcgttt acaggcttga aaagattcgc
    13981 aagctcacag gtctcgacct cagagagttc gacgacgcca tagtattcaa agtagcatta
    14041 atggttaaaa agtatctcga tgtcacaccc tccaaattct aaacttaata gcttaaaaca
    14101 atacggcaaa cctgttttat acgggtttgc tgtgattgct ttaaaaggtt ttttggtaaa
    14161 tttttcataa tcggaaaaga gacggattaa ttattgattt aattaaagga tgttataata
    14221 gctttgaaat acacttgaat ccctttcaag gtttacatat agaggtttaa taatgattga
    14281 gttcaggaac ttgaccaaaa tatatccaaa cggcaccaaa gccctaaagg atgttaacat
    14341 aaaaatagaa aaaggcgagt ttgtgttcat agtcggcgcg tcgggcgccg gcaagagcac
    14401 ccttttaaaa attatgatgc gtgaggaggt cgccacctca ggctcggtta taatcaacaa
    14461 ctacaatctc aacgcaataa aaaagaagga tatcccgaaa ttcaggcgca ccatgggcat
    14521 tgtttttcag gatttcaggc tcatccccaa tcttagcgcc tacgacaatg tagcatttgc
    14581 aatgagagtt atcggcgcga aggagaggga agtgcgcaaa agagttactc ataccttaag
    14641 ccttgtggga ctttcctcaa aggctaagtg cctgcccgcc cagctctcgg gcggcgagca
    14701 gcagagggtc gctcttgcaa gggctcttgt aaacaacgcg gatttgatta tagcggacga
    14761 gcccacgggc aatattg
//
LOCUS       DAONVQ010000034        14706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3080_length_14706_cov_4.510545, whole genome shotgun sequence.
ACCESSION   DAONVQ010000034 DAONVQ010000000
VERSION     DAONVQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14706
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3080_length_14706_cov_4.510545"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..869)
                     /locus_tag="PLD98_03520"
     CDS             complement(99..869)
                     /locus_tag="PLD98_03520"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQA05351.1"
                     /translation="MLLVVDAGNTNITIGVYDGDKLLSVSRLYTDRGKTKDQYAVEIL
                     EIMKLNGINMPEFTGAIVSSVVPEITRILIKAIKQITGLDAVCVSVENSAGLIIDIDS
                     PTQLGADLIATAVAAREKYPLPCVVIDLGTATKISLVDEGGIFRGCAIAAGVGISLKA
                     LAAETSQLPAISLDAPESAIGKNTITAMQSGAVLGTAYMLDGLAVRFQNEIGEKVSIV
                     ATGGYSSDIVPHCEKDILLDPTLLLEGLKIIYESSINS"
     gene            1141..1938
                     /gene="proC"
                     /locus_tag="PLD98_03525"
     CDS             1141..1938
                     /gene="proC"
                     /locus_tag="PLD98_03525"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HQA05352.1"
                     /translation="MKIAFLGVGNMAQPIIQGIINRGVLEPENVVLFDVDTEKMNSFA
                     ESVGAAAADSAESAVKAADILMLAVKPQIMQNLLTELSAVLEEKKPSIISIAAGKTTE
                     FIQSCLNYEAKIARIFPNLNAKVSESISAFCGNKNADADFLSVVEKIALSFGKAIALD
                     EEYFPVFGVLAGCAPAYTFLYIKSLCDAAKEAGLPEDLAYKTAVQVVLGSAKLLSSVS
                     ESPETMIDRVCSPGGTTIEGIKSLKENSFQEIVSKAFNASLNRDKSL"
     gene            2027..2335
                     /locus_tag="PLD98_03530"
     CDS             2027..2335
                     /locus_tag="PLD98_03530"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HQA05353.1"
                     /translation="MSDEYGPDIVSVIDEEGREHVFEELDRIETDEGKYVALIPVYDD
                     PEESLEDSGELIILKVEEEDGEIYLCPIEDEEEFETVGNIFEDRLIEFYELEKESEEE
                     "
     gene            2358..2545
                     /gene="ssrS"
                     /locus_tag="PLD98_03535"
     ncRNA           2358..2545
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLD98_03535"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2645..4114
                     /locus_tag="PLD98_03540"
     CDS             2645..4114
                     /locus_tag="PLD98_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HQA05354.1"
                     /translation="MKEFFIALLILLAAFIVITLIRAAFFRPKKRDSEPLPPEKVDVD
                     RFVKNLSDAIKIKTICNRNPEKVDWSEFEKFHAFLEERYPFIHKTLQKEVVSKASLLY
                     KWQGKNPNLKPIALLSHQDVVPVTEGTEKDWKYPPFEGTVAEGYIWGRGAIDMKNHLI
                     GVMESIETLLEEGFQPERDVYLCFGHDEEIMLSDNSGAITMARLLKERGVELESVIDE
                     GGAIIPAKIKGLLDINIAGVGIAEKGYADFEISVSAKGGHSSQPPNHTALGEMANIIK
                     DIENHQFKAKLTPYIKELFIRIGKQMTYPGRIFTCNLWLLWPVVKAVMKRIPAAASMI
                     RTTTAVTMASGSPAPNVLPQTAKIVANFRIMPGSSVKDVEKHLIKVIKNKNVNIRLIE
                     SKEPSAISPTDSEAFKAIEDIFYRMNNKNLVAPYLVMGGTDACRYEGVAKNIYRLSPF
                     VFSTDLLLTTHGTNERLPIDCVEDGVAFFKRYIRRLAGE"
     gene            complement(4167..4430)
                     /locus_tag="PLD98_03545"
     CDS             complement(4167..4430)
                     /locus_tag="PLD98_03545"
                     /inference="COORDINATES: protein motif:HMM:NF019085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HQA05355.1"
                     /translation="MGVKLFYLLFALGGLAIIIASIRTGHFLKSLILTAVQGIAALFA
                     VNFIGSFFGVHINLNPFSLIVSSVAGTAGVILLLLLNSLALKT"
     gene            4572..5543
                     /locus_tag="PLD98_03550"
     CDS             4572..5543
                     /locus_tag="PLD98_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HQA05356.1"
                     /translation="MIKNYKKEFVFDFDTAPTPSCHASTVLPLDDGTVLAAWFGGTAE
                     GKDDVVIWVSRRTENGWEKPIQVSDSDNIPHWNPVLHLRQDGSICLYFKVGKKVAVWQ
                     TYYSISTDNGKTWTKQQVLVPGEKIDGRGPVKNKCLRLSNGTLLGPASTEQKRMWRCF
                     IDISEDDGRTWTMSPFIVRPRTTFGVVGMIQPTLWEGPEGHVHALMRTDKGKIYRSDS
                     TNYGRTWCKAYPTSLPNNNSGIDLVKVPDGRIFLLYNNVADNWGKRNPLTLAVSTDNG
                     DTWENIMDLETDDPGEFSYPAITYKDGSLHMTYTHNRERIAYWKMDI"
     gene            5558..6538
                     /locus_tag="PLD98_03555"
     CDS             5558..6538
                     /locus_tag="PLD98_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA05357.1"
                     /translation="MRYATIGTSWITESYIDGANSVPDTDLYAVYSRDEEKAKAFAEK
                     HGAKKYFSSLEEMASCKEIEGVYIASPNSLHYEQSRMMLNAGKHVICEKPIAINPVEV
                     EELISLADKKGLVYMEAIMMLHSPALKLLKQAIGEIGKVTTAHIDFCQLSSKYPAYLR
                     GETPNIFNPDLGTGCLMDIGVYNVYFALELFGIPEKIYATGKFMRTGADACGSAIFEY
                     PDKLVTLNYSKVSQSRTPSYINGDEGSVEVGSISQLTDIYKVNKAGEKTLTFGDSTRH
                     TLMENEARNFYNFITEPEKHKDEYEHAKQIALNVSKVMKEIRNQVKGFKF"
     gene            6557..7474
                     /locus_tag="PLD98_03560"
     CDS             6557..7474
                     /locus_tag="PLD98_03560"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HQA05358.1"
                     /translation="MKIIPNGVYPTMITPFTDDNKIDYNAVEQLIQWYADRGVAGIFA
                     ICQSSEIFFLSFEEKLELLKFIVDKAPKGVSIIASGHTADDIETQIEEAKAICDVGID
                     AYVFISNRFAAPEESDDVLLERMTKVANALPEDIGLGVYECPYPYKRILSPYLLKEMS
                     KNPRYQFLKDTCCSIEMIKAKLDAVKGTNLRIFNANSATLLESLRLGCAGFSGVMANF
                     HPELYAELCEIYESNPEKAEKIQNFVGFASVAEYQLYPVNAKYHLKLTGIDMGIHTRS
                     KDATKFTESQRKEIEQLYKLTEMFKEMIK"
     gene            7709..8701
                     /locus_tag="PLD98_03565"
     CDS             7709..8701
                     /locus_tag="PLD98_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HQA05359.1"
                     /translation="MKKSDLNALPPLVVEFLRYIGSIKNMSNSTVSEYAFDLRTFIRF
                     VKAEREGISLDELESIDMKDCDIDFFSSITLTDAYAFLSYCKDERGNSAKTRARKVAS
                     IRAFYKYLHVTKKLIRENPMAELDSPKLSKTLPKYLTLDQSIELLNSIDGKNKERDFC
                     IITLFLNCGLRLSELVSLNYTDIRSDNTMRVRGKGSKERTVYLNDACIQAINDYMKVR
                     PKDGVIDREALFLSNRLKRISPKTVQYIVKTFIEKSGLGGHGFSTHKLRHTAATLMYQ
                     HGNVDILVLKEILGHDNISTTEIYTHVIDKHIKEAVNANPLSDFKKDKSNKNGK"
     gene            8843..10714
                     /locus_tag="PLD98_03570"
     CDS             8843..10714
                     /locus_tag="PLD98_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HQA05360.1"
                     /translation="MYYNTGNLCIGCMQPLGDSSTCLNCHTERSYVQPSPYLPLRTVI
                     DNKYLVGKVQDYNGDGVTYIGLDLEKNNRIFIREFFPQGAAFRSTNNINLMPMSGKEM
                     VFEDCHQSFLALWRQIARLRGLSAIQSVYDIVDANGTSYIISDYIDGITLRDYLLRSS
                     IGYITWKQARTLFLPVLSTLGTLHSAGIIHRGISPSTILVNQDDGKLILSGFSIWQAR
                     SMQGPLTPEIFSGYAPIEQYAGTDAKQGPWTDIYSFAAVLYRALIGSTPIDAPSRVEN
                     DTLMIPAKFAEQLPAYVINSLINALQISPEDRTRTVEELRAELSASPTAVAASASSYF
                     DETPPSPAPTAPVREPVRGKPDKSKTSVVVLTVIIVLLALAAIGIILALTVFRDRLGL
                     TTEPNNTVIETTTGTESTAPSGMVIVPNFVDMTKSSVEASQDIKKNFVIEWKEEHSDV
                     VPKDFIIAQSIAANTQVAIGTKIVLTVSLGVSTVKIPDVTNLPFEQAKTILEDLGLVC
                     VRDVKVNDGSKIGGTVAETIPGINTEVKIGDEVIVVVWQELETKPVTVTVPLFINESV
                     NSIITNPEWKDVFKFNFVYEYNSSVPKDFVISQSLPSGSEVTAPAEITLKISLGSSD"
     gene            complement(10755..12128)
                     /locus_tag="PLD98_03575"
     CDS             complement(10755..12128)
                     /locus_tag="PLD98_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HQA05361.1"
                     /translation="MSKYSEILAKQREFFDTGQTLDPFFRKKQLQLLKRAIKQNEQAI
                     NEALRLDLNKAPLEAYTTEVGFILQELGYMINSVLRLAAPKMVLSPLIHFPSSTMVYN
                     EPYGSVLIIAPWNYPLQLAFAPLIGAIAAGNCAVIKTSQYAPNCSKIIAKIISETYDP
                     AYITVVEGEPGASEAILEEKFDYIFFTGSVEVGKSVMAAAAKNLTPVTLELGGKSPCI
                     VTENANIKLAAKRIVWGKFLNSGQTCIAPDYILVSEKIKDELITQLKRFIEKQYTSDP
                     LTYADYPKIVNERHFNRLLRLIEGEKIISGGKSDRDKLKIEPTLLDEPAADSPVMSEE
                     IFGPILPIISFDTIENAAKYIKKRPKPLALYVFSECKDEQKYFVKNVSFGGGTINDTI
                     IHISTSAAGFGGVGDSGMGKYHGKASFETFSHSKTVMKKGNWLDFPYRYHPFSDFNLK
                     VVKFFMH"
     gene            complement(12219..13175)
                     /locus_tag="PLD98_03580"
     CDS             complement(12219..13175)
                     /locus_tag="PLD98_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA05362.1"
                     /translation="MDTFFNFLYASIIASTPLLFGTVGEILSEKVGNMNLGVEGMMWL
                     GAFAGFYAAYKTESFLISLLASFGLAAVGALIYAFLTVTLKANQNVTGLTLTTFGIGL
                     SLVFGKAMITAAGGAPKTSDVFNAKVAAINIPVLSDIPYIGKIIFGLNPYVYIAVAIA
                     IILYVYLMKTKTGLNLRAIGENPAAADASGINVNLMKYINIIIGGGICGIGGAYMSLV
                     MVNGSWQDSGVVNGYGWIAVALVIFSSWNPAKAILGSLIFGMFSSLRYYVPSDIIVIP
                     TAFYQMLPFVVTTIVLIVTSIKKNKENTQPAACGINYFREER"
     gene            complement(13175..14284)
                     /locus_tag="PLD98_03585"
     CDS             complement(13175..14284)
                     /locus_tag="PLD98_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA05363.1"
                     /translation="MSNGSKVSTGREPLIRIVKREYNNKLIPGLIRAGALILSIIAGG
                     LFIWAMGFNPFEAYKSIIRGALVGSARDPMSSIRSTIDIFVPLCVTALGLSCAFKMKF
                     WNIGGEGQIIMGGVFATFFALNYPQIPRPLMLLIMLIAGAVGGALMALIPALFKVKFG
                     TNETLFTLMLNYIAIYIVKYLIAGPWQRTPGFASIGKLDKNTYLYEINRQNIGWIAVI
                     IVVLFVYFYLYKTKSGYEVSVVGESQATARYAGMNIRKIIIKTMLISGAICGIVGMIK
                     VSSNHTLTEGIGGGVGFAAITVAWLSQLNPFIIPIVSAIISILQKGSTVMQMNMGISS
                     YAADVLQGIILFCFLGSEFFVRYSLVFNLRIKEAK"
     gene            complement(14277..>14706)
                     /locus_tag="PLD98_03590"
     CDS             complement(14277..>14706)
                     /locus_tag="PLD98_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05364.1"
                     /translation="LVINRKPSRELAEKLVKRLEIVTPSIKTPVSRLSGGNVQKVLIG
                     REIESNPNILITAYPVRGLDINSSFTIYNLLNEQKAKGVAVLYIGEDLDVILELCDRV
                     MVLCHGEVMGIVDPRKVTKEDIGLMMLGNKLEKEGGAENE"
BASE COUNT         4342 a         3242 c         3257 g         3865 t
ORIGIN      
        1 ggggataaag gtcatggcga gaacaaccgc agaaataatc gaagtggcaa caagatagaa
       61 caatttgttt ttattcttca ttgcaacaac ctaccttctt aactatttat actgctttca
      121 tatattatct ttaaaccctc aagaagaagg gttgggtcga gtaaaatgtc tttctcgcag
      181 tggggaacga tgtccgaaga ataaccgccc gttgctacaa tacttacctt ttcaccgatt
      241 tcgttttgga accttacggc aagaccgtca agcatatacg cggtgccgag aacagctccc
      301 gactgcatgg cggtaatagt gttcttgccg attgcggatt cgggagcgtc aaggctgatg
      361 gcggggagct gagaagtctc agcagccaaa gcttttaagg agattccgac gcccgcggca
      421 atcgcacagc ctctgaaaat tccgccctca tcgacaaggc ttatcttcgt agcagtgccg
      481 aggtctataa ccacacaagg gaggggatat ttttctctcg cggcaacggc ggtagctatc
      541 aaatccgctc caagctgggt aggggagtcg atatctataa taagacctgc gctgttttcg
      601 acgcttacac agacggcatc aagacctgta atctgcttta tagctttaat caatattctt
      661 gtgatttcgg gtacaaccga gctgactatt gcgccagtaa actctggcat atttatgccg
      721 ttcagcttca taatctccaa aatttcaaca gcatactggt cttttgtttt gcctctgtcc
      781 gtataaagcc ttgatacaga cagcagcttg tcgccgtcat aaacacctat tgtaatgttg
      841 gtgtttccgg catcgactac taagagcaca atgctcactc cttaaagtaa cggaatatac
      901 atagactata cacattttaa taaaaattat ataatgatta tacactgcat ttttttgcca
      961 ttcaatagat tttttgtaca aataaaagct tttattttct tgatagtata ttaaaacatg
     1021 atataattaa ttttaatagt gttacaaagt ctttgctata cagcatgtat aattattatg
     1081 atttaaaatg caaaggctaa gttttgtttt taattgcgaa tacataatgg ggatgagagt
     1141 atgaaaattg cttttttggg cgttggcaac atggcacagc cgataatcca aggaattata
     1201 aacaggggag tattggaacc cgaaaatgtg gtgctttttg atgttgacac cgagaaaatg
     1261 aacagtttcg cagaaagcgt cggagccgcc gcggcagaca gcgctgaaag cgccgtaaag
     1321 gcagccgata ttctaatgct tgccgtaaag ccgcagatta tgcaaaatct tctaaccgag
     1381 ctttccgcgg tattagagga aaaaaaaccg tcaataattt ctatagcggc aggcaaaacg
     1441 actgagttta ttcagtcctg cttaaattac gaggcaaaaa tagcaaggat tttcccgaat
     1501 ctaaacgcta aggtttccga gtccatatcc gcgttctgcg gcaataaaaa tgccgatgcc
     1561 gacttccttt ccgttgtaga aaagatagct ctcagcttcg gcaaggcaat agctctggat
     1621 gaggagtatt tccccgtatt cggtgttctt gcgggatgcg cccccgctta taccttcctt
     1681 tatatcaaaa gcctttgcga cgcggcaaaa gaggcaggct tgcccgaaga tttggcatat
     1741 aaaaccgctg ttcaggtggt tttgggcagc gcaaagctcc tttcatcggt ctctgaatct
     1801 cccgaaacta tgattgaccg tgtttgctca ccgggaggaa caacgataga gggcattaaa
     1861 tcccttaaag aaaattcatt tcaggaaatt gtttccaaag catttaatgc ctcattaaac
     1921 agggacaaga gcttgtaacg catagctttt tatacagtgt ttaacagttg acactaaaca
     1981 gaaagcattg taaaataaca tcaagagaag ttaaggagtt gtttatatgt ccgatgaata
     2041 tggtcccgat atcgtcagcg ttatagatga agagggacga gagcatgtct tcgaggaact
     2101 tgacagaatc gagaccgacg aaggcaaata tgtagcgcta atacccgttt acgatgaccc
     2161 cgaagagtct ttggaggact ccggcgagct tataatactc aaggttgagg aagaagacgg
     2221 cgaaatctat ctctgcccga ttgaagatga ggaagaattc gaaaccgttg gaaatatttt
     2281 tgaagacaga ctcatagagt tttatgaatt agaaaaagaa tctgaggaag aataatcttg
     2341 agttcagtat ttatctgacc tccctgcttt gttcgataaa tacggtaaat ataacccaac
     2401 acacacttaa agggagctga tctccatctg tggattgcag gcctcccata gcaatatgga
     2461 cgaaggaagc gtgaagcaga agggtaagca cccacctgcc aaatagcagg ttattatcta
     2521 ccgttttccg gcatagcggg ggcttttttt atgcccaaaa atagaatgtg ccatttcttt
     2581 atataattta ttgacattta taatgcggat gttagaataa acttaactga tcggaggttt
     2641 tgctatgaag gagtttttta ttgcgctact tattttgctt gcagcgttta ttgtcataac
     2701 cctcattaga gctgcgtttt tcagaccgaa aaagagggac tctgaacccc tcccgcccga
     2761 aaaggttgat gtggatagat tcgtaaagaa tctctccgac gctataaaga ttaaaaccat
     2821 ctgtaaccgc aaccccgaaa aagtggattg gtcggagttt gagaaattcc acgctttcct
     2881 tgaggagaga tatcccttta tacataaaac tttgcagaag gaagttgtaa gcaaagcgag
     2941 ccttctgtat aaatggcagg gcaagaatcc taacttaaag cccattgccc ttctttcaca
     3001 tcaggatgtt gttcccgtta ccgaaggcac cgaaaaggac tggaaatatc ctccctttga
     3061 aggcactgtc gcagaaggct atatttgggg gcgcggcgcc attgatatga aaaaccatct
     3121 catcggcgtt atggagagca tagaaaccct gcttgaagaa ggattccagc ccgaaagaga
     3181 tgtctatctg tgctttggtc atgatgaaga aattatgctc agcgacaatt cgggagccat
     3241 tacaatggct aggctgctta aagagagggg agttgagctc gaatccgtaa ttgacgaggg
     3301 cggagccata atccccgcaa aaataaaggg tcttttagac attaacattg caggcgtagg
     3361 cattgctgaa aaaggctatg ccgactttga aatctccgtc agcgcgaagg gcggacactc
     3421 ctcccagccc ccgaatcata cagcactcgg cgagatggcc aatatcatta aggatattga
     3481 gaatcatcag ttcaaggcaa agctcactcc ctatattaag gaactgttca tccgcatagg
     3541 aaagcagatg acctatcccg gaaggatatt cacctgcaat ctgtggcttt tgtggcctgt
     3601 tgtaaaggca gttatgaaaa gaatccccgc tgccgcttca atgattagga ccactacagc
     3661 cgtaacaatg gcatcgggca gccccgcgcc caatgttctg cctcagacag cgaagatagt
     3721 cgcaaacttc agaatcatgc ccggctcctc agttaaagat gtggaaaagc atctcataaa
     3781 ggttataaag aataaaaatg ttaacataag gctgattgaa agcaaggagc cttccgcaat
     3841 atctcccacc gacagcgaag ccttcaaggc tattgaggac atcttctaca ggatgaataa
     3901 caaaaacctt gtggcaccct atcttgtaat gggcggcaca gacgcttgca gatacgaagg
     3961 agttgctaag aatatttaca ggctttctcc ctttgttttc agcaccgatt tgctgcttac
     4021 cacccatggt acaaacgaaa ggctgccgat tgactgtgtt gaggatggag tcgccttctt
     4081 taaaagatac attcgccgcc ttgcgggaga ataaatataa aagaaaagcc gcgatgcaaa
     4141 acatctgggc tctttgtttt gcgcttttag gtttttaagg ctaatgagtt aagcagcaaa
     4201 aggagaatta cgcccgctgt acccgcaacc gaggatacaa tcagcgaaaa aggatttaag
     4261 ttaatatgta cgccgaagaa cgagcctata aaatttaccg caaagagcgc cgctataccc
     4321 tgaacggcgg ttaagattag gctctttaaa aaatgacctg ttctgatgga ggcaattata
     4381 attgcaagac cgcccaatgc aaacaaaaga taaaacagct taactcccat aatttatcac
     4441 cactaatcta tattctattt ttcggacaaa tataccatat aatgtggttt aggacaaaaa
     4501 ataataatat gttgacatag agaaatataa tagtaaaatc aaaaagagca aaaaattcgg
     4561 gagggaacaa tttgataaaa aactataaaa aagaattcgt atttgacttt gacacggctc
     4621 ccacaccttc atgccacgca tccacagttc ttcctctgga tgacggcact gttctcgccg
     4681 catggttcgg cggaacggct gaaggcaagg acgatgtggt aatctgggta tcaagaagga
     4741 ccgaaaacgg ctgggaaaag cctattcagg tttccgattc cgataatata cctcactgga
     4801 accctgttct tcatttaagg caagacggaa gcatttgtct gtattttaaa gtgggcaaaa
     4861 aggtcgctgt atggcagacc tattactcaa tttcaacaga caacggcaag acctggacaa
     4921 agcagcaggt tcttgttccc ggcgagaaga ttgacggcag aggtcccgtt aagaataagt
     4981 gcttaagact ttcaaacggc actctgcttg gccccgcatc cacagagcaa aagcgtatgt
     5041 ggagatgctt tatcgacatt tccgaggacg acggccgcac atggacaatg tctcccttta
     5101 tagtaagacc ccgcaccaca ttcggcgttg tcggcatgat tcagcctaca ttgtgggaag
     5161 gccctgaagg acatgttcac gctcttatgc gcacagataa gggcaagatt tatcgcagcg
     5221 actcaacaaa ctacggcaga acatggtgca aggcatatcc cacatctctg cccaacaaca
     5281 acagcggcat tgaccttgta aaggttcccg acggacgaat tttcctcctt tacaacaatg
     5341 tcgcggacaa ctggggcaag cgcaatccgc tgacattggc cgtttccact gataacggag
     5401 acacctggga gaacattatg gaccttgaaa ccgatgaccc gggcgaattc tcttatcccg
     5461 caattacata caaagacggt tcgctccata tgacatacac ccacaaccgt gagagaatcg
     5521 cttactggaa aatggatata taatagggga gtctgacttg agatacgcaa ccataggaac
     5581 atcctggata accgaaagct acattgacgg agcaaactca gtacccgata ccgatcttta
     5641 cgcggtatat tcgagggatg aagaaaaggc taaagctttt gccgaaaagc acggagcaaa
     5701 gaagtatttt tcatcccttg aggaaatggc aagctgcaaa gagattgaag gagtttatat
     5761 agcgagcccg aattctctgc attatgagca aagccgcatg atgctaaacg cgggcaagca
     5821 tgtaatctgc gaaaaaccca ttgccataaa tcctgtggaa gtagaagagc tgattagcct
     5881 cgccgacaaa aaaggccttg tttatatgga ggctataatg atgctccatt ctcccgcttt
     5941 gaaacttctt aagcaggcga taggggagat aggaaaggtc acaacagctc atatcgattt
     6001 ttgtcagctt tcgtcgaaat atccggcgta tctgagaggg gagaccccga atattttcaa
     6061 tcccgacctt ggcacaggct gtcttatgga cataggcgtt tataatgtct attttgcgct
     6121 tgagttgttt ggaattcccg aaaagattta tgccacaggc aaattcatga gaacaggcgc
     6181 ggatgcctgc ggttcggcga tttttgagta tcccgacaaa ctggttactt taaactattc
     6241 aaaggtttct caaagcagaa ctccttcata catcaacggc gacgaaggct ctgttgaggt
     6301 cggctcaata tctcagctta ccgatattta taaggtaaat aaagcgggtg aaaagactct
     6361 tactttcggc gacagcacaa ggcatacgct tatggaaaac gaagcccgta acttttataa
     6421 tttcataacg gagccagaaa agcataagga cgagtacgag cacgctaaac aaatcgctct
     6481 gaatgtcagc aaggttatga aggaaatcag aaatcaggtt aaaggtttta aattttaaaa
     6541 agcgaaggag agcaaaatga agataatccc caacggcgta tatcccacga tgattacgcc
     6601 ctttacagat gacaacaaga tagattacaa cgctgttgaa cagctcattc aatggtatgc
     6661 ggacagaggc gttgcgggta tatttgcaat ctgccagtcc agcgagattt tcttcctcag
     6721 ctttgaggaa aaactggagc ttttaaagtt catcgtagac aaggctccca aaggtgtttc
     6781 aataatcgca tctggtcaca ccgccgacga tattgaaact cagatagaag aagcaaaggc
     6841 aatctgcgat gtgggaatcg acgcttatgt ctttatttcc aacaggtttg ccgcgcccga
     6901 ggaatcggac gatgttcttt tagagagaat gacaaaggtc gcaaacgcgc tgcctgaaga
     6961 tattggtctc ggcgtatacg aatgtcctta tccttataaa aggattcttt ctccttatct
     7021 cctcaaagag atgtcaaaaa atcccagata tcagttcctt aaagacacat gctgcagcat
     7081 agaaatgatt aaggcaaagc tggacgccgt aaaaggtaca aacctcagga tcttcaacgc
     7141 aaactcggca acgctgcttg agagcttgag acttggctgc gcaggcttct cgggcgttat
     7201 ggcaaatttc catccggagc tttacgctga gctttgcgaa atctatgagt caaatcccga
     7261 aaaagccgaa aaaatacaga attttgtcgg atttgcgtcc gttgctgaat atcagcttta
     7321 tcccgtaaac gccaagtatc atttaaaact gacaggcatt gatatgggaa ttcacacaag
     7381 atcaaaggat gcgaccaaat ttacagaaag ccaaagaaaa gaaattgaac agctttataa
     7441 gctgacagaa atgtttaaag aaatgattaa ataaatacat agcccgagct tttgctcggg
     7501 ccattgttat aagaaatttt aagacttgtc aattttaagg acagctttaa atatattata
     7561 attataatat ataatgtttt aagtcggcag ctttaaagcc gcaaagctca aagacttatt
     7621 gaactcaaaa attctgattc cccatgccct aaattataca aaatctttat tttgtataat
     7681 tagtactttc aaacaggcga agtgaaaaat gaagaaaagc gatttaaatg cccttccgcc
     7741 tcttgttgta gaattcttaa ggtacatagg ctcaattaag aatatgtcaa actccacggt
     7801 cagcgaatac gcctttgacc ttagaacctt tatacgcttt gtaaaggctg agcgcgaagg
     7861 aatctctttg gatgagcttg aatcaatcga tatgaaagac tgtgacattg atttcttcag
     7921 cagcatcaca cttacggacg cttacgcatt tttgtcttac tgcaaggacg agcgcggaaa
     7981 ttccgcaaaa acaagagcaa gaaaggttgc gtcaataagg gccttttata aataccttca
     8041 tgtcaccaaa aaactaatta gagaaaaccc aatggccgag cttgacagcc ccaagctctc
     8101 gaaaacgctt cccaaatatc tcacacttga ccaaagcatt gaattattaa actcaattga
     8161 cggaaaaaat aaagaaagag atttctgtat tattactctt ttcttaaact gcggacttcg
     8221 cctttccgaa ttggtttcac taaattatac cgatataagg tctgacaata cgatgcgtgt
     8281 caggggcaaa ggctcaaagg agcgcaccgt atatcttaac gatgcctgca ttcaagctat
     8341 aaacgactat atgaaggtcc gcccgaagga cggcgtaatt gacagagagg cactgttttt
     8401 aagcaaccgc cttaaaagaa tcagccccaa aaccgttcag tatattgtaa aaacctttat
     8461 tgaaaaaagc ggtctcggcg gtcacggttt ttcaactcat aaattaaggc ataccgccgc
     8521 gactttaatg tatcagcacg gaaatgtcga cattttggtc ttaaaggaaa tattaggtca
     8581 cgacaatatt tcaaccactg aaatatatac ccatgtaatt gacaagcata taaaggaggc
     8641 ggtaaacgcc aatccccttt cggacttcaa aaaagataaa agcaataaga atggaaaata
     8701 atagaaaatt ggttacatga gtatcacaaa atgttacagc tcatttgcat atcttgccaa
     8761 ggtttttaag ttgtgataat atacattaga cgaaaattgc cccttatttg atgcattcta
     8821 aacaatattt tgaggtggat atatgtacta caacaccggc aatctatgta ttggttgcat
     8881 gcagccgctt ggcgactcat caacatgctt gaattgccat acagagcgtt catatgtaca
     8941 gccatcaccg tatttgcctc tgcgcacagt tattgataat aaatatcttg tcggcaaggt
     9001 tcaggattat aacggagacg gtgtaactta tataggcctt gaccttgaaa agaacaacag
     9061 aatttttata agagaattct tcccgcaggg cgcggctttc aggtccacaa ataacataaa
     9121 ccttatgccc atgtcaggca aagaaatggt ttttgaagac tgccatcaga gcttccttgc
     9181 tctctggcgt cagattgcga gactcagagg tctttcggca attcagtccg tttacgatat
     9241 tgttgacgcc aacggcactt cttatatcat ttctgattat atcgacggca tcacattaag
     9301 ggattacctt ttaagaagca gtatcggcta tattacatgg aaacaagcgc gtacgctttt
     9361 cctgcccgtg ctttctaccc taggcaccct tcattcagcc ggcatcatcc acaggggcat
     9421 atcaccgtca acaattcttg taaatcagga tgacggaaag ctgattcttt cgggattcag
     9481 catctggcag gcaaggtcaa tgcagggccc cttaactccc gaaatcttct ccggctacgc
     9541 tcccattgag cagtacgccg gaacagacgc aaagcagggt ccctggaccg acatctactc
     9601 ttttgccgct gtgctttaca gagctttaat cggcagcact cccattgacg ctccgtcaag
     9661 ggttgagaat gataccctta tgattcccgc aaagtttgcg gaacagctgc ctgcttatgt
     9721 aattaattcg cttattaacg cgcttcagat ttctcctgag gacagaaccc gtacggttga
     9781 agaactgaga gcggagcttt ccgcctcccc caccgccgtt gcggcaagcg caagctcgta
     9841 ttttgacgaa acacccccct cccctgctcc gactgctccg gttagggaac ccgtaagggg
     9901 taagcctgac aaatccaaaa cgagtgttgt ggtactgact gtcatcatcg tactccttgc
     9961 tctcgccgcg attggaataa tacttgcgct caccgtattt cgtgaccgcc tgggtcttac
    10021 cacagagcca aacaacacgg ttattgaaac cacaaccggc accgaaagca ctgctccgtc
    10081 aggcatggta attgtgccga actttgtcga tatgacaaag agcagtgtag aagcatccca
    10141 ggatataaag aagaactttg taatcgagtg gaaagaagag cattccgatg ttgttcccaa
    10201 agacttcatt atagcccaga gcatcgccgc aaatactcag gtggcaatag gcacaaagat
    10261 tgtgctcaca gtaagtcttg gcgtttcaac cgtaaaaatt cccgatgtga ctaacctgcc
    10321 ctttgagcaa gccaaaacca ttttggagga tttggggctc gtttgcgtgc gtgatgtaaa
    10381 ggtcaacgac ggcagtaaga ttggcggcac cgttgccgaa acaataccgg gcataaatac
    10441 cgaggttaaa atcggtgatg aagtaatcgt agtggtatgg caggagcttg aaaccaagcc
    10501 cgtaaccgta acagtgccgc tgttcataaa cgaaagcgtt aattccatca tcacaaatcc
    10561 tgaatggaag gatgtattca aattcaattt cgtttatgag tacaactcaa gcgtgccgaa
    10621 ggattttgta atatcccagt ccctgccctc aggcagcgag gtaaccgccc ccgctgagat
    10681 tacactcaaa atcagcctgg gaagcagcga ctaaaagaac aaataaaaag cccgctgaat
    10741 gcgggcttta atttttagtg cataaagaat ttgacgacct taaggttgaa atcgctgaag
    10801 gggtggtacc tgtaggggaa gtcgagccag ttgccctttt tcattaccgt cttgctgtgg
    10861 gagaaggttt caaagctggc cttgccgtgg tatttgccca ttccgctgtc ccctactccg
    10921 ccgaatcctg cggcagatgt gctgatgtga attattgtat cattaattgt tccgccgccg
    10981 aatgaaacat tcttaacaaa atacttttgc tcatctttgc actctgagaa tacatacagc
    11041 gcaaggggct tcgggcgttt tttaatatac tttgccgcgt tctctattgt atcaaaggag
    11101 ataatcggaa gaatgggtcc gaagatttcc tcgctcatga ctggcgaatc cgcagcgggt
    11161 tcatcaagga gcgtcggttc gattttaagc ttatccctgt cggacttgcc gccagaaata
    11221 atcttctccc cttctatgag ccttaaaagc ctgttaaagt ggcgctcgtt aacaatcttc
    11281 ggataatcgg cgtaagtcag cgggtcgctt gtgtactgct tttcaataaa ccttttaagc
    11341 tgagttataa gctcgtcctt aatcttttcg gaaacaagaa tatagtcggg ggctatgcag
    11401 gtctgacccg aatttaagaa tttgccccat actattctct ttgcggcaag ttttatattg
    11461 gcattttcag ttacgatgca aggactcttt ccgccaagct caagggttac gggcgtaaga
    11521 ttcttagctg cggctgccat aacgctcttg ccgacctcaa cactgccggt aaagaaaata
    11581 tagtcaaact tctcttccaa aatggcttcg gatgctccgg gctcaccttc cacgaccgta
    11641 atataggcgg ggtcataggt ttcactgatt atcttagcga ttattttgga gcagtttgga
    11701 gcgtactgtg atgttttgat tactgcgcag tttcctgccg cgattgctcc gatcagcggc
    11761 gcaaaggcaa gctggagagg ataattccac ggagcaatta tcagtaccga gccgtaaggc
    11821 tcattgtaaa ccattgtgct cgacgggaaa tgtataagcg gggataatac cattttggga
    11881 gccgcaagcc ttaaaacact gtttatcata tagccgagtt cctgaagaat aaaaccgacc
    11941 tcggttgtat atgcctctaa gggcgctttg ttaaggtcaa gtcttaatgc ttcatttatg
    12001 gcttgttcgt tttgcttaat cgccctttta agaagttgca gctgcttttt tctgaaaaat
    12061 gggtccaggg tttgtccggt gtcaaagaat tctctttgct ttgccaaaat ttcagagtat
    12121 ttgctcattt tatttcctcc caaagtagtc tgtagaaaca ttatatcaca aaaaaaagaa
    12181 aaataaaatc ccacactgta aaagtgtggg atttttgctt atctttcttc tctgaagtag
    12241 ttaatgccgc atgcggcggg ctgagtgttc tctttgttct ttttgattga agttacaatc
    12301 agaacaatgg ttgttaccac aaacggcagc atctgataga aggctgtcgg gataacgata
    12361 atatcagagg gaacatagta tctcagcgag ctgaacatgc cgaaaatcag cgagccgagg
    12421 attgcctttg cgggattcca gcttgagaag ataacaagag caacagcaat ccagccgtaa
    12481 ccgttaacaa cgcccgaatc ctgccaactg ccgttaacca ttaccaatga catataagct
    12541 ccgccgatac cgcaaatacc gccgcctatg ataatgttaa tatatttcat aaggtttaca
    12601 ttgatgcccg aggcgtcagc tgcggcgggg ttttcgccaa tagccctgag gttaagtccc
    12661 gttttggttt tcataaggta gacataaagg atgatggcga ttgcaaccgc aatataaaca
    12721 taaggattaa ggccaaagat gattttgccg atatagggaa tgtcggaaag tacgggaata
    12781 tttattgctg cgacctttgc attgaaaaca tccgaggttt tgggtgcgcc gcctgcggct
    12841 gtaatcatag ccttgccgaa aacaagggat aaacctatgc caaaagttgt aagcgtaaga
    12901 cccgtaacat tctgatttgc cttgagagta acggtaagga atgcgtaaat caaagcgccg
    12961 acagcggcaa gtccgaagga ggcaaggaga gaaataagaa aactctctgt cttatatgcc
    13021 gcatagaagc cggcaaacgc gccgagccac atcataccct caacaccgag gttcatattt
    13081 cccacctttt cggagagaat ctcgcccacg gtgccgaaaa gcaacggcgt tgacgcaatg
    13141 atggaagcat agaggaaatt aaagaaggta tccattattt tgcctccttt attctgaggt
    13201 taaatacaag cgaatatctt acaaagaact cactgccgag gaagcagaac agtattatac
    13261 cctgcagaac atcggcggca tatgaggata ttcccatatt catctgcatt acggtgctgc
    13321 ctttttgcag tatgctgatg attgctgaaa caatcgggat tataaaggga ttgagctgcg
    13381 agagccacgc gacggtaatt gcggcaaagc ctacgccgcc gcctatgccc tctgtgagag
    13441 tgtggttgga tgaaacctta atcatgccga ctatgccgca gattgcgccg cttatgagca
    13501 ttgtctttat tataatcttg cggatgttca tgcctgcgta tcttgcggtt gcctggcttt
    13561 cgcctactac gctgacttca tagccgcttt tggtcttata aagatagaag taaacgaaca
    13621 atacaactat tataacggca atccagccta tgttctgtct gtttatctca tagagataag
    13681 tgtttttatc cagttttcct atgcttgcaa agccgggcgt tctctgccac ggacccgcga
    13741 ttaggtattt gacgatgtaa attgctatat agttcagcat caaggtaaac agtgtttcat
    13801 tggtgccgaa ttttaccttg aacaacgcgg ggataagagc catcaaagca ccgcccactg
    13861 cgccggctat gagcattatg agaagcatta agggtctcgg aatctgaggg taattaaggg
    13921 caaagaatgt agcgaaaact ccgcccataa taatctgacc ctcgccgcct atattccaga
    13981 acttcatctt gaatgcgcat gaaagtccta gggctgttac gcacaaggga acgaaaatgt
    14041 ctattgtgga gcggattgat gacatcgggt ctcttgctga acccacaagc gcgcctctga
    14101 ttatggactt atatgcctca aagggattga agcccattgc ccaaatgaac aaaccgcccg
    14161 caataatcga aagaatcaag gcgcccgcgc gaataaggcc ggggataagc ttattgttat
    14221 attcacgctt tactattctg ataagcggct ctctgcctgt gcttaccttt gaaccattac
    14281 tcattttcgg cacctccttc tttctccagt ttattgccaa gcatcattag accaatatct
    14341 tcttttgtaa cctttctggg gtcaactatg cccataacct caccgtgaca caaaaccata
    14401 actctgtcgc aaagctcaag aataacatca aggtcctcgc ctatatacag aacggcaacg
    14461 ccttttgcct tctgctcatt aaggagattg taaatcgtaa acgaggagtt aatgtcaagt
    14521 cctcttacgg gataggcggt aatgagaata ttcggattgc tctcgatttc tctgcctatg
    14581 agcaccttct gaacattgcc gccagaaagc ctgctgacgg gtgtttttat gctgggagtt
    14641 acgatttcaa gccgcttaac cagcttttcg gcaagctccc ttgaaggctt tctgtttatt
    14701 acaagc
//
LOCUS       DAONVQ010000035        14627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3109_length_14627_cov_4.160925, whole genome shotgun sequence.
ACCESSION   DAONVQ010000035 DAONVQ010000000
VERSION     DAONVQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14627
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3109_length_14627_cov_4.160925"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="PLD98_03595"
     CDS             complement(<1..1303)
                     /locus_tag="PLD98_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQA05365.1"
                     /translation="MKGKNSVFKKGHEFFSAILSQLLSAAKSFKSRKRKIAYGFLSLV
                     LAVSMLPLGAFGTLAAEADYAAQIGTTKYETLNAAFDAVKISQTITLLKDVVVDAPIN
                     VSGIKSFTFDLNGKTVESNKSKTDTISSLFYLNFDGNFAIQDSSSKKEGKIIGYPSFN
                     ADDSVFCVNKGTLVLNGAAIEAVNLNNDGKPNIGSAVLLKNSGKLTMYDGTISANTKN
                     TVYASDSSSAIVYAGLVENLNMSGNNGIAMDFRSGGTLELKGGTIYSKKYFALYIAYS
                     GAKVIVSGGTVRSEFAGAAHILGQGLEFLVSGGSFEGYTENNYGYAVTVGSGSIAVFT
                     GGEVCNTGNGRAIGGYTDKIYIAGGSPVFRTYDLSKKNMSAVENMNIFDLTDYLGDCY
                     YSYYSDGRTLEQYTGGSAVRKYITFKEDGHTPIKLNLETNGG"
     gene            complement(1476..2042)
                     /locus_tag="PLD98_03600"
     CDS             complement(1476..2042)
                     /locus_tag="PLD98_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05366.1"
                     /translation="MKKTLALTLAILMAFAMLLSFAACGGTDDAEETTAPAKTELVTA
                     SVYGLSFKVPADFTEFTEKQPGAKSANKGAGASIAVTDIIDASELPFDSWTEENYISD
                     TLAGTTDAKILEFSKDKKVSGYDALYVHYTTKSQSGTELEGYNYVIYYPLQDGGTGYQ
                     SITFAMVKSVESSLTANITEVLESLSAE"
     gene            2400..3113
                     /locus_tag="PLD98_03605"
     CDS             2400..3113
                     /locus_tag="PLD98_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQA05367.1"
                     /translation="MLRIAICDDEARSALDTKALLEQWSAETGHETELHCFDNGDSLI
                     NETCSEKYDIIFLDIVMPLLSGMDTAKELRLKDKSVKIIFLTSSAEFALQSYAVKASD
                     YCLKPITYSRLKEIMDDCLASINRESESLTLKTAGGFQKIYLHEIEYIEAQNKRVFFF
                     LKNGKAVEVVQQLYTFESVLSCSKGFFKCHRSYLVYLPNVDNFNSIEIKTKSGRRIPI
                     ARGYGKAFKDAYFAVMFKD"
     gene            3125..3427
                     /locus_tag="PLD98_03610"
     CDS             3125..3427
                     /locus_tag="PLD98_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05368.1"
                     /translation="MLEFFMGMLTNTALLLFGIFASAAILGISFTKRNIGVLLLFCLV
                     INAVQFYFYSILGLRGAQWMYPIMTHVPSVLLFTLHLSEGYTPAFLRCSPLIFAVS"
     gene            3397..4392
                     /locus_tag="PLD98_03615"
     CDS             3397..4392
                     /locus_tag="PLD98_03615"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HQA05369.1"
                     /translation="MLSAYLCCQLSKWLGLLVLEISGKMWISYAVRCAATIAIGYVII
                     RYFASSIAVILTKPVKTVLIFGILPAAYYLFDYIATVYTDLLYSGSEVVFEFLPFILC
                     IAYLFFSIVYFKEYEEKCEAEQYNRLMELKRTQSEKEIEAIKRSEHAVSILRHDMRHF
                     MNNIVVLINQGDSEKAKAYISEIIKSADKTATQRYCENEIVNMIISSYENQLKELEIN
                     LCHSIQIPRKLHISDIDLTSILSNGIENAINAVAALEKDQRRITLDLRMNDDKLLLSI
                     KNPFAGRVEMTDGIPRAKGDGHGLGTQSIKYITEKLSGNCQFLAKDGQFALRVVL"
     gene            complement(4461..5057)
                     /locus_tag="PLD98_03620"
     CDS             complement(4461..5057)
                     /locus_tag="PLD98_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HQA05370.1"
                     /translation="MGLKERYYSTLVVSASESFNATISELLPEAHCSPVHMVSSISEA
                     KRSIAEREYDFVFINAPLPDEYGVRFSIDVSAIKSSVTVLLVRSDIFAETLDRVTEHG
                     VFVLSKPTSRQMLIHALEWMTSAREKIRMHQSKTQTLEEKMEIIRLVNRAKLLLISEM
                     HMDEAQAHRYIEKQAMDRCVSRKVIAEEIIRTYTSTQG"
     gene            complement(5079..6290)
                     /locus_tag="PLD98_03625"
     CDS             complement(5079..6290)
                     /locus_tag="PLD98_03625"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HQA05371.1"
                     /translation="MKYSKEEVLQYVKEEDVKFIRLAFCDVFGRQKNISIMAHELPRA
                     FEYGIAFDASAINGFGNEARSDLLLHPQAETLMLLPWRPEHGKVVRMFAHITYPDKRP
                     FESDTRNLLVKTVMKAEAEGYRFAFGVEQEFYLFKLDENGKPTKEPYDEAGYMDIAPD
                     DRGENIRREICLTLEQMGIRPESSHHEQGPGQNEIDFRYSDPITAADNAMTFQTVVRT
                     VAHRNGLYADFSPKPLADQPGNGFHINMSVKADDGSNNLHYMIAGILEKADQMTAFLN
                     PTENSYKRLGVQKAPGYISWSSENRSQLIRIPAAEGEYNRAELRSPDPTANPYLAFTL
                     MIEAGLYGIKNRLDLPPEVDSNLYKADAKILSKLKPLPKDLETACKLAAKSSFIKQCL
                     PEAILNSYLSR"
     gene            complement(6311..7030)
                     /locus_tag="PLD98_03630"
     CDS             complement(6311..7030)
                     /locus_tag="PLD98_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HQA05372.1"
                     /translation="MVINVRELGNKELNELLYKYKGEITLEECCGQRFIGAGMSDSFI
                     SINGVPGNALGAYLNGSKIVVNNNAQDAVGDTMNDGEIVVYGDIGDTAGYAMRGGKIF
                     VKGNAGYRAGIHMKEYKEKKPLMVIGGRAGSFLGEYQAGGVIVVLGLTESEKPIVGSF
                     PCTGMHGGKIFLRSDCKDIKFPDQVTARKADKKDLKELEEYVSEFCGLFGYNFKEVMN
                     SPFKFVTPDSKNPYRQMYVYN"
     gene            complement(7034..8266)
                     /locus_tag="PLD98_03635"
     CDS             complement(7034..8266)
                     /locus_tag="PLD98_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA05373.1"
                     /translation="MKNFVIIGNGVAAVGCIEGIRSIDKESKITVISEEKHFVYGRPL
                     ISYFLENKTDLERMKYRDSDFYEKMGCEVLYGRRAEKIDTDSKRVILDNGTEVPYTSF
                     CVAAGSSPFIPPFEGLDSVKKKFTFQTLDDALELEKAINADSKVLIVGAGLIGLKCAE
                     GIYGRVESITVCDLADRILSSIFDADSSEMMKKHIESKGIKFILGDTVSRFEKNKAIM
                     KSGKEVKFDILVIAVGVRPNTALVKEIGGEVNRGIIVNDRMETSLPDIYSAGDCTEGN
                     DITAGEKKVLALLPNAYMQGHAAGVNMAGGDEVFDKAIPMNSIGFFGLHAMTAGVSDG
                     EVYEEKTDTSLKRLFTKDDRLVGFILIGITERAGIYTSLIREKTPLSTLNFDLLKKIA
                     TTASFAPEIRRKKFGGVV"
     gene            complement(8263..8703)
                     /locus_tag="PLD98_03640"
     CDS             complement(8263..8703)
                     /locus_tag="PLD98_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQA05374.1"
                     /translation="MKRIYVNENWCLGCHLCEYYCAFANSGMSDMAKALKDKPIYPRI
                     HIEGDERISYAVSCRHCTDPICVKSCIAGAIKQTDGVVQIDREKCVGCLTCVLVCPYG
                     ALSPDENGVMQKCELCLKNSFGEPACVKGCPNKAIVYEERGETE"
     gene            complement(8707..10212)
                     /locus_tag="PLD98_03645"
     CDS             complement(8707..10212)
                     /locus_tag="PLD98_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HQA05375.1"
                     /translation="MSVQLINPEFEVIRNENRCIACRVCERQCANEVHSYDEDRKMMV
                     ADESKCVNCQRCVALCPTRALKIVKNDCVLRENANWRNDTIREIFMQANSGGVLLSSM
                     GNPKDYPVYWDKILINASQVTNPSIDPLREPMETKVFLGKKPEKIERDENGRIKCSLP
                     PQIELSMPIMFSAMSYGSISYNAHASLARAAKELGILYNTGEGGLHEDFYQYGDNTIV
                     QVASGRFGVHKDYLEAGVAIEIKMGQGAKPGIGGHLPGAKIVGDISRTRMIPEGTDAI
                     SPAPHHDIYSIEDLRQLVYSLKEATQYKKPVIVKVAAVHNIVAIASGIARSGADIIAI
                     DGYRGGTGAAPTRIRDNVGIPVELALAAVDQRLRDEGIRNNVSLVAGGSIRSSADVVK
                     AIALGADAVYIATAALIALGCHLCRTCHSGKCNWGIATQRPDLVKRLNPDIGCERLVN
                     LLKAWQHEIKEMMGGMGINSIEALRGNRHMLRGIGLTEKELEILGISHAGE"
     gene            complement(10209..11306)
                     /locus_tag="PLD98_03650"
     CDS             complement(10209..11306)
                     /locus_tag="PLD98_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05376.1"
                     /translation="MLLEGQIRIPSGCAIAAVISRGGKKMTGEMIIEGMKKMHDRSNG
                     LGGGFAAYGIYPDYKDFYALHMFFDSRTTRKNCEAFLKERFEIVESELIPTRKIPAIT
                     DEPIIWRYFVAPLKSVLNSMQLDEKEFVVRTVMQINDEMDGAYVFSSGKNMGTFKAIG
                     YPEDVGVFYKLDEYEGYSWTAHGRYPTNTPGWWGGAHPFTLLDWSVVHNGEISSYDAN
                     RRFIEMFGYKCTMKTDTEVITYIMDYLLRIQKLTFKEAANVVAAPFWSEIEDLADPKE
                     KEELYFLRAIFPSLLITGPFSIVMGFNGGLMALNDRLKLRSLVVGEKDDKVFIASEEA
                     AIRRMEPDAENVYFPAGGEPVIVQIEDGAFR"
     gene            11671..13005
                     /gene="gdhA"
                     /locus_tag="PLD98_03655"
     CDS             11671..13005
                     /gene="gdhA"
                     /locus_tag="PLD98_03655"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HQA05377.1"
                     /translation="MSYVDEVIKRVERDCPNEPEFHQAVKEVLNSLRPVVDANEALYR
                     REALLERLVIPERHVEFRVSWIDDNGNVQVNTGYRVQFNSAIGPYKGGLRLHPSVNLS
                     IVKFLGFEQIFKNALTGLPLGGGKGGSNFDPKGKSDREVMAFCQSFITELYKYIGADT
                     DVPAGDIGTGAREIGYMFGQYKRLKGVWEGVFTGKGLSYGGSLVRTEATGYGLLYILE
                     EMLKCKGIALEGKKILVSGSGNVAIYAIEKAHQLGAKVLTCSDSNGWVYDPEGIDLAA
                     LKEIKEVKRQRLTEYKKYRPNSEYHEGRGVWSVKADIALPCATQNELNLEDAKMLVNN
                     GVFAVAEGANMPTTAEAVDFLLENGVYFLPGKAANAGGVATSGLEMCQNSGRLSWSFE
                     KVDENLKKIMIDIYHNIDNAAKKYGLEGNYVAGANIAGFEKVANAMIAQGAV"
     gene            13114..>14627
                     /locus_tag="PLD98_03660"
     CDS             13114..>14627
                     /locus_tag="PLD98_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HQA05378.1"
                     /translation="MKTVKDYFGSLVFDDRVMKEMLSADVYAKLKKTMDEGSELDISV
                     ANAVADAMKEWAISKGATHFTHWFQPLTGITAEKHDSFIAPNPHGGVIMEFSGKELIK
                     GEPDASSFPSGGLRATFEARGYTAWDPTSYAFIKGKTLCIPTAFCSYEGHALDKKTPL
                     LRSMEALNRQALRILRLFGNTSAKHVRACVGPEQEYFLVTKEMYDKRPDLRFTGRTLF
                     GARPPKGQEMDDHYFGAIKPRVAAFMEELNEELWKVGILAKTEHNEVAPAQHEIAPIY
                     TTANIATDHNQLTMEIMQKVALKHGLVCLLHEKPFAGVNGSGKHNNWSIATDSGQNLL
                     SPGDTPYENAQFLLFLCAVIKAVDDYQDLLRISVATAGNDHRLGANEAPPAVVSIFLG
                     DELTAILDAIESGTPYNGTSKIEMKLGVDFLPKFHRDTTDRNRTSPFAFTGNKFEFRM
                     LGSSNSIACANIMLNSAVAESLCQFADRLESAGNFEAELNSLIKDTIKAHKRII"
BASE COUNT         3589 a         3479 c         3569 g         3990 t
ORIGIN      
        1 tgccgccgtt tgtttcgagg ttgagtttta tcggggtgtg gccgtcttct ttaaaggtta
       61 tgtactttct gacagcggag ccgcccgtgt actgctccag agttctgcca tcgctgtaat
      121 agctgtaata gcagtcgccc aagtaatctg tcaggtcgaa aatattcatg ttttcaacag
      181 ccgacatatt tttttttgac aggtcgtagg ttctgaacac cggagagccg ccggcgatat
      241 agattttatc cgtgtaaccg ccgattgctc tgccgttgcc cgtattgcag acctcaccgc
      301 ctgtgaaaac ggctatcgag ccgctgccga cagtaacggc ataaccatag ttattctctg
      361 tgtaaccttc aaaagaaccg ccgcttacca agaactcaag gccctgacct aaaatatgcg
      421 ctgcgccggc aaattccgag cggacagtgc cgcctgaaac aattactttc gcgccgctgt
      481 aggcgatata aagggcaaag tatttcttag agtaaattgt gcccccttta agctcaagcg
      541 tgccgcctga acggaaatcc atagcgatac cgttatttcc gctcatgttt aggttttcaa
      601 ccagacctgc gtaaacaata gcggaagaag aatctgacgc gtaaaccgta ttttttgtat
      661 tggcggaaat tgtgccgtcg tacattgtca gcttgccgct gttttttagc agtacggcag
      721 agccgatatt cggttttccg tcattattca gattaacagc ttctatcgcg gcgccgttta
      781 aaacaagagt gcctttattt acgcaaaaaa cagaatcatc cgcattaaaa ctcgggtagc
      841 ctatgatttt gccttctttt ttagagctag agtcttgtat cgcaaaattg ccgtcaaaat
      901 ttaaataaaa gaggctgctt attgtgtcag tcttgctttt gttgctctca acggttttgc
      961 cgttaaggtc aaaggtaaaa ctctttatac cgctgacatt aatcggagcg tcaacaacaa
     1021 catcctttaa aagggtgatt gtttgactta tctttactgc gtcaaatgca gcattaaggg
     1081 tttcgtattt cgtcgtgccg atttgggccg cataatcagc ctcggcagca agtgtgccga
     1141 acgctcccaa gggcagcatg gaaactgcaa gcacaaggct taaaaagccg tatgcaatct
     1201 ttctttttcg ggacttaaag ctctttgcgg cactcaaaag ctgtgagagg attgccgaaa
     1261 aaaattcatg tccctttttg aacacgctgt ttttaccttt cattctcttt gctccaaact
     1321 aagataaatt gaaaacaaaa aatctcgaag tgattataat agaaacaata aggtcaaatt
     1381 tccttccttc gtgaaacgca ttatttttgg agcgaaatgc attgaaaaac atagaaaagc
     1441 cgactgcggt ccgcagtcgg cttaattctt tgatgttatt cggcgctgag gctctccaga
     1501 acttctgtga tgttagccgt taaggaggat tccacgctct ttaccatcgc gaaagtaatg
     1561 ctctgatagc cggtgccgcc gtcctggagg gggtagtaaa ttacatagtt gtagccttca
     1621 agctctgtgc cgctctgaga cttggtggtg taatggacat agagggcgtc atagccggaa
     1681 acctttttgt ccttgctgaa ctcaaggatt tttgcgtcag tggtgccagc aagggtatct
     1741 gagatgtagt tttcttcggt ccatgaatca aaaggcagtt cgctcgcgtc aattatgtcc
     1801 gttaccgcaa tgctggctcc cgcgcctttg tttgccgatt tcgcgcccgg ctgcttttcg
     1861 gtgaactcag taaaatcagc gggaaccttg aaggacagtc cgtaaacgga agcggtgaca
     1921 agctctgtct ttgcgggggc ggttgtttcc tctgcgtcgt cggtgccgcc gcatgccgca
     1981 aagctcaaca gcattgcaaa tgccataagt atagcgagtg ttagcgcaag tgtttttttc
     2041 ataagttttc tccttaaata tatttacgat tgcgttttct ttaatgtctt atgcttttct
     2101 aaagcaaacg gaggaaagat tattaaagat taagcaatct gctcttttaa acgcggcaaa
     2161 acgcgtctaa aggattatag ccgtatttat cttccttgaa cggtttttgc gtgagctgca
     2221 gaaatttagg agtgaaatgc agaaaacaat gcctgaatta cccaatatat cctttattgt
     2281 tctttttcgg atgacgctta atgaactctt tgcatcggct ttgccgctgt attttttcat
     2341 tgaacaatta aaaaaaatat gctaaactgt atttgatact aaataaagtg agaatttaaa
     2401 tgctgcgaat agctatatgc gatgatgagg caagaagcgc tttggataca aaagccttgc
     2461 ttgaacaatg gtcggcggag acggggcacg aaactgagct tcactgcttt gacaacggcg
     2521 attctttaat aaacgaaacc tgctcagaaa agtacgacat catctttctt gatattgtga
     2581 tgcccctcct tagcggcatg gataccgcaa aagagctgag actcaaggac aagtcggtaa
     2641 aaatcatctt tttgacatcc tctgccgagt ttgctttgca gtcttacgcc gtaaaggctt
     2701 ccgactactg tttaaagccg attacatatt caaggctcaa agaaattatg gacgactgcc
     2761 ttgcctcaat aaatcgcgag tctgagagtt taactcttaa aaccgcagga ggctttcaga
     2821 aaatctatct tcatgaaatc gaatacatag aggctcagaa caaaagagtt ttcttctttt
     2881 tgaaaaacgg aaaggctgtg gaggtagttc agcagctata tacctttgag tcggttctgt
     2941 cttgctccaa gggctttttt aaatgccatc gcagttacct tgtttatctt cccaatgtgg
     3001 ataatttcaa cagcatcgag ataaaaacca aatcaggcag gcgcatcccg attgcgagag
     3061 gttacggcaa agcatttaaa gatgcgtatt tcgctgtaat gtttaaggat taggaggtcg
     3121 cgccgtgctt gaatttttta tgggtatgct gacaaacact gccctgcttc tttttggcat
     3181 attcgcgtca gcggcgattc tcggcatcag ctttaccaaa agaaacatcg gggttttgct
     3241 gcttttctgc cttgtcataa acgcggtgca gttttatttt tacagcatct tggggctcag
     3301 aggcgcccag tggatgtacc ccattatgac ccatgtaccc tctgtgcttt tattcaccct
     3361 gcatttaagc gaaggttaca cgccggcatt tttgcggtgc tctccgctta tctttgctgt
     3421 cagctgagca aatggctggg gctgctggtt cttgaaatca gcggaaagat gtggatttct
     3481 tacgccgtaa ggtgcgctgc gaccatagcg atcggctatg tgattatccg ctatttcgcc
     3541 tcgtcaatag ccgttattct cacaaagccc gtaaagacgg ttttgatttt cggcattctc
     3601 cccgcggctt attatctgtt tgactatatc gccaccgttt acactgacct gctttacagc
     3661 ggctctgagg ttgtgttcga gtttctgcca tttatacttt gcatcgccta ccttttcttc
     3721 agcatcgtgt actttaagga gtacgaggaa aagtgcgagg ctgagcagta taaccgcctt
     3781 atggagctga aaaggacaca gtcggaaaag gaaattgagg cgattaagcg ttctgagcac
     3841 gcggtttcaa tactcaggca cgatatgcgg cattttatga acaatatcgt ggttcttata
     3901 aatcaagggg attcggaaaa ggcaaaggct tatatcagcg agattatcaa gtccgccgac
     3961 aagaccgcta cgcagagata ctgcgagaat gaaattgtga atatgataat ctcctcttat
     4021 gaaaatcagc ttaaagagtt agagataaac ctctgccatt cgatacagat acccagaaaa
     4081 cttcatatct ccgacattga cctgacctcc attctctcaa acggcataga gaacgcaata
     4141 aacgctgttg cggcgctgga gaaagaccaa aggagaatta cgctggattt aaggatgaat
     4201 gatgacaagc tgcttttgtc aatcaaaaat ccctttgcgg gcagggttga aatgacagac
     4261 ggcatacccc gggctaaggg agatgggcat gggctgggaa cacagagcat taagtacata
     4321 acggagaaac tcagcggcaa ctgccagttt ttagctaaag acggtcagtt cgctttgaga
     4381 gtggtactgt aaataaaaag accgcagcag aaaagctgcg gtttgttttt ttatgtcttt
     4441 gcggagcttt acccgctttt ttagccctga gtgcttgtat atgttcttat aatctcttcc
     4501 gctataactt tgcgggagac gcatctgtcc atagcctgtt tttctatata gcggtgggcc
     4561 tgagcctcat ccatgtgcat ttcgctgata agcagcaact ttgccctgtt gacaagacgg
     4621 attatctcca tcttttcctc aagggtctgg gttttgctct ggtgcatcct gattttttcc
     4681 cttgcgcttg tcatccattc aagggcgtga atcagcatct gccttgaggt gggctttgaa
     4741 aggacaaaca cgccgtgctc cgttaccctg tcaagggttt cggcaaagat gtcggagcgg
     4801 actaaaagca ccgttacaga gcttttaata gcgctgacat ctattgaaaa gcgcactccg
     4861 tactcatcgg gaaggggcgc gttgatgaat acaaaatcat attccctctc cgcaatggac
     4921 cgttttgcct cgcttatgct tgacaccata tgaacggggc tgcagtgggc ttcggggagc
     4981 agctcggaga ttgtggcgtt aaagctctct gaggctgaaa cgactagagt gctgtaatag
     5041 cgttccttta agcccatttg ctcccctcct aagttttatt atctgctcaa gtaggaattg
     5101 agaattgcct ccggcagaca ttgtttaata aaactgctct ttgccgccag cttgcaggct
     5161 gtttcaaggt ccttgggcag cggctttaat tttgaaagaa tttttgcgtc tgctttgtaa
     5221 aggttggagt cgacctccgg cggcaggtca agcctgttct ttatgccgta caaacctgcc
     5281 tcaatcatta aagtaaaggc aagataggga tttgccgtcg ggtcgggcga acggagttct
     5341 gctcgattgt attctccctc cgcagcgggt attcggataa gctgggagcg gttttcgctc
     5401 gaccatgaaa tatatccggg cgctttttgc acgcctaaac gcttatagga attttcggtg
     5461 ggatttaaga atgcggtcat ctggtccgct ttttcaagaa ttcccgcaat catatagtgg
     5521 agattgtttg agccgtcgtc cgccttgaca gacatattta tgtggaagcc gttgcccggc
     5581 tggtccgcaa ggggcttggg agaaaaatca gcgtaaagcc cgttgcggtg agctaccgtt
     5641 ctgacaacgg tttggaaggt catcgcgttg tccgccgcgg tgatggggtc tgagtagcgg
     5701 aagtcgattt cgttctgacc gggaccctgc tcgtggtgag agctttcggg gcgaatgccc
     5761 atctgctcta aagtcaggca gatttcacgg cggatgttct ctcccctgtc gtcgggggcg
     5821 atgtccatat agccagcctc gtcatagggc tcttttgtgg gcttgccgtt ttcgtcaagt
     5881 ttaaagaggt agaattcctg ctcaacgccg aaagcgaagc gatagccttc agcctctgcc
     5941 ttcataacgg tttttacaag caggtttctc gtatcgctct caaagggtct tttgtcggga
     6001 taggttatat gggcgaacat tctgacgacc ttgccgtgct caggtctcca gggcaggagc
     6061 ataagggttt ccgcctgggg atgaagcaaa aggtcagaac gggcctcgtt gccgaaaccg
     6121 tttattgctg aagcatcaaa ggcaatgccg tattcaaagg cgcggggaag ctcatgcgcc
     6181 attattgaaa tgttcttttg cctgccgaaa acatcgcaga aggcaaggcg gatgaactta
     6241 acatcctcct ccttgacata ctggagtacc tcttcctttg aatacttcat aacaaaacct
     6301 tccttttgat ttaattatac acatacatct gcctgtacgg gtttttgctg tcgggtgtga
     6361 caaacttaaa gggagaattc attacctctt taaagttata tccgaaaagg ccgcagaatt
     6421 cggatacata ttcttccagc tctttaaggt cctttttatc cgcttttctg gcggttacct
     6481 ggtcggggaa ttttatatcc ttacagtcgg accttaaaaa gattttgccg ccgtgcattc
     6541 ccgtgcaggg gaaggagccg acaatgggct tttcgctctc agtgagaccc aaaacgacga
     6601 taacgccgcc tgcctgatac tcgcccaaaa agctgcccgc tctgccgcct atgaccataa
     6661 gcggcttttt ctctttgtac tctttcatgt gtatgcctgc tctgtagcct gcattacctt
     6721 tgacaaagat ttttccgccg cgcatggcat agcccgctgt gtcgccgata tcaccgtaga
     6781 ctacgatttc accgtcgttc atggtgtcgc ccacggcgtc ctgcgcgttg ttgttgacaa
     6841 caatctttga gccgttaaga taggctccga gggcgttgcc gggaactccg tttatgctta
     6901 taaagctgtc ggacataccc gcgccgataa agcgctgacc gcagcactcc tcgagtgtaa
     6961 tttctccctt gtatttataa agcagctcgt ttaactcttt attgccgagc tctcttacat
     7021 taattaccat atattatacc acacctccga acttttttct acgaatttca ggcgcaaatg
     7081 aggcggttgt agcgattttt ttgagaaggt cgaaatttaa tgtgctgagc ggggtttttt
     7141 ctctgattaa tgaggtataa atgcccgctc tctctgtaat gccgatgaga atgaatccta
     7201 caagacggtc gtcttttgta aacagacgct ttaaggaggt gtcggttttt tcttcgtaaa
     7261 cttcgccgtc gctgactccc gcggtcatgg cgtggagccc gaaaaagccg attgagttca
     7321 tggggatagc cttgtcaaaa acctcgtcgc cgcctgccat gttaacgcct gcggcatgac
     7381 cctgcatata ggcgttgggc aaaagggcta aaactttctt ttctcccgcc gttatgtcgt
     7441 ttccctctgt gcagtcgccg gcggaatata tgtcagggag gctcgtttcc atgcggtcgt
     7501 ttacaattat gcctctgttt acctcgccgc cgatttcttt gacaagggcg gtgttggggc
     7561 gcacgccgac ggctattaca agaatatcga actttacctc tttgccgctt ttcatgatag
     7621 ctttattctt ttcaaagcgg ctgacggtgt cgccgagaat gaatttaatt ccctttgact
     7681 cgatgtgctt tttcatcatc tcggagctgt cagcgtcaaa aatgctggag agaatacggt
     7741 cggcaaggtc gcagacggta atgctctcca ctctgccgta gattccttcg gcgcatttaa
     7801 gacctattag acccgcgccg acaatcagaa cctttgagtc agcattaatt gccttttcaa
     7861 gctccaaggc gtcgtcaagg gtctggaagg tgaatttctt ttttacactg tcgagccctt
     7921 caaagggcgg gataaagggt gatgagccgg ctgcgacgca aaatgaggtg tatggaacct
     7981 ccgtgccgtt gtcaaggatt accctttttg agtctgtgtc tattttctcc gctctcctgc
     8041 cgtaaagcac ctcgcagccc attttttcat aaaagtctga gtcgcggtac ttcatgcgct
     8101 caaggtcggt tttgttctcg agaaaatagg aaatgagagg tctgccgtaa acaaagtgct
     8161 tttcctcgga aataacggtt attttgctct ctttgtcaat gctgcggatt ccctcaatgc
     8221 agccgaccgc ggcaacgccg ttacctatga ttacgaaatt cttcattcgg tctcacctct
     8281 ctcctcataa actattgcct tgttggggca tccctttacg catgcgggct cgccgaaaga
     8341 gtttttaaga caaagctcgc atttctgcat tacgccgttc tcgtcgggcg aaagggctcc
     8401 gtaggggcag acaagaacgc aggtaaggca gccgacgcat ttttctctgt caatctgcac
     8461 cacgccgtcg gtctgcttaa tggcacccgc aatgcagctt tttacgcata tggggtctgt
     8521 gcagtgcctg catgacaccg cgtatgaaat tctctcgtcg ccctctatgt ggattcgggg
     8581 atagatgggc ttatctttaa gcgcttttgc catgtcgctc attcccgagt ttgcaaaggc
     8641 gcagtagtat tcgcaaagat gacagccaag acaccaattt tcgttaacat agattcgttt
     8701 cataggctat tctcccgcat gagaaattcc gagtatctcc agttctttct cggtaagacc
     8761 gattcctctg agcatgtgcc tgttgcccct caatgcctcg attgagttaa tgcccatgcc
     8821 gcccatcatt tctttgattt cgtgctgcca tgcctttaag agattcacaa ggcgctcgca
     8881 gccgatgtcg ggattaaggc gctttacaag gtcggggcgc tgggttgcga tgccccaatt
     8941 gcacttgccg ctgtggcagg ttctgcaaag gtggcagccg agggcaatca gcgccgcagt
     9001 tgctatatac accgcgtcag cgccgagggc tattgccttt accacatcgg cggagctgcg
     9061 tatgctgccg cccgccacaa gggaaacatt gttgcggata ccctcatcgc ggagcctctg
     9121 gtcaacggcg gcaagagcga gctcaacggg aatgcccaca ttatcgcgga ttcgtgtggg
     9181 agccgctccc gttcctcctc tgtaaccgtc gatggcgata atgtcggcgc cgctccttgc
     9241 gatgccgctg gcaatcgcca cgatgttgtg aacggcggca acctttacga tgacgggctt
     9301 tttgtactgg gttgcctctt taagggagta aacaagctgc cttaaatcct cgatggaata
     9361 aatatcgtga tggggcgcgg gagaaatggc gtcggtgccc tcgggaatca ttcttgtgcg
     9421 ggaaatatcg cccacaattt tggctccggg gagatgtccg ccgatgcccg gttttgcgcc
     9481 ttgacccatc ttaatttcta tggcaacgcc cgcctcaaga taatccttat gaactccgaa
     9541 gcgtccagat gccacttgaa ctatcgtatt gtcgccgtat tggtaaaagt cctcgtgcag
     9601 tccgccctcg cccgtgttgt aaaggattcc gagctctttt gcggctctgg cgagcgaagc
     9661 gtgggcgttg tagctgattg agccgtagct catggctgag aacataattg gcattgaaag
     9721 ctctatctgg ggcggaaggc tgcatttgat tcttccgttt tcgtcccttt cgattttttc
     9781 gggctttttg cccaggaaaa cctttgtttc cataggctct ctcagggggt cgattgacgg
     9841 gtttgtaacc tgagaggcgt ttataaggat tttatcccag tagacaggat agtctttggg
     9901 attgcccatt gatgaaagga gtactccgcc gctgtttgcc tgcatgaaga tttctctgat
     9961 ggtgtcattt ctccaatttg cgttttcgcg gagtacgcag tcattcttta ctatctttaa
    10021 agcccttgtg gggcaaaggg cgacgcagcg ctgacaatta acgcactttg attcgtccgc
    10081 caccatcatt tttctgtcct cgtcgtagct atgtacctcg ttggcacatt gtcgctcaca
    10141 gacacggcag gctatgcaac ggttttcgtt gcgtataacc tcgaattcag gatttataag
    10201 ttgaacgctc atctaaaagc accgtcctca atctgtacta ttacgggttc gcctcccgcg
    10261 gggaaataga cattctccgc atcgggctcc atacggcgta ttgccgcctc ctcactggct
    10321 atgaaaacct tgtcatcctt ttcgccgact accaatgaac gaagtttaag gcggtcgtta
    10381 agagccataa gtccgccgtt gaaacccatt acaatggaga acgggcctgt gattaaaaga
    10441 ctcgggaata ttgctctgag gaaataaagc tcttcctttt ctttggggtc agccaaatcc
    10501 tcaatctcgc tccagaaggg cgcggcgacc acattcgcgg cttctttgaa agtcagcttc
    10561 tgaattctga gcaaatagtc cattatatag gtaataacct cggtatcggt tttcatcgtg
    10621 catttgtagc cgaacatttc gatgaatctg cggtttgcgt cgtatgagga gatttcgccg
    10681 ttgtgtacca ccgaccagtc aagaagggta aaggggtggg ctccgcccca ccagccgggg
    10741 gtgttagtgg gatagcgtcc gtgggcagtc caggaatagc cctcgtactc atcaagttta
    10801 taaaagacgc ccacatcctc gggatagcct atcgccttga aggtacccat gtttttgccg
    10861 ctggagaaca cataagcgcc gtccatttcg tcgttaatct gcattacggt gcggacgaca
    10921 aattcctttt cgtcaagctg catggagtta agcaccgact ttaaaggcgc tacaaaatac
    10981 ctccagataa taggctcatc tgttattgcg gggattttgc gtgtagggat aagctcagac
    11041 tccactatct caaaacgctc ttttaaaaaa gcctcacagt tttttctggt agttcgggaa
    11101 tcaaagaaca tatgaagggc ataaaagtcc ttatagtcgg gatatatacc gtaagcggca
    11161 aagccgccac caagtccgtt tgaacggtcg tgcatttttt tcattccctc aattatcatc
    11221 tcgccggtca tttttttgcc gcctcttgaa attactgcgg caattgcaca acctgagggg
    11281 atacgaattt gaccttcaag caacatatgg gtctttcctt tcgagtaaaa taaaaaaggc
    11341 gctcataaaa gcgcgtgaag cgctgttatg aacgccattg ctcattttta gttattataa
    11401 tcgatttgcg gctcttttgc aacaatatat agaaaaaaat tacaactttc tctaaaaatt
    11461 gctgtttggg tcttgacaaa gggcttttcc aatacatata atgttgtggt aagaagcgaa
    11521 tggagtttaa gcaacgacgc ttaaactgta acttacaatt gaatattttt taggttggta
    11581 ttaaaggcaa tggcgtcttt agtggtgttt tccgcaccat taagggcgca tttttatttg
    11641 tcttggaaag tagaaagagg ttgtcagcga atgtcctatg ttgatgaagt aattaagagg
    11701 gttgaaagag attgccccaa cgaacccgag ttccatcaag ccgtaaagga ggttttgaat
    11761 tccctaagac ctgtagttga cgcaaacgaa gctctttaca gacgcgaggc tctgcttgaa
    11821 aggcttgtta ttcccgagcg ccatgttgaa ttccgagttt catggattga cgataacgga
    11881 aatgttcagg tcaacaccgg ttaccgtgtt cagttcaaca gcgctatagg tccctataag
    11941 ggaggtttga gacttcaccc gtcggtaaat ctcagcattg ttaagtttct cggctttgag
    12001 cagatattta aaaatgccct gacaggtctg cctttgggcg gcggcaaggg cggctcgaat
    12061 tttgacccca agggcaaatc ggaccgcgag gttatggctt tttgccagag ctttataacc
    12121 gagctttata agtacatcgg cgccgacacg gatgttcccg caggcgacat cggcacggga
    12181 gcaagagaaa tcggctatat gttcggccag tacaagaggc tcaaaggcgt atgggaaggc
    12241 gtatttacgg gaaaaggtct ttcttacggc ggctcgcttg taagaaccga agctacaggc
    12301 tacggacttc tgtatattct tgaggagatg ctcaaatgca agggcatcgc tcttgaggga
    12361 aagaaaatcc ttgtttccgg ctcgggcaat gttgctattt acgcaattga aaaggcgcat
    12421 cagctcggcg caaaggtact cacctgctcc gactcaaacg gctgggtata tgaccccgaa
    12481 ggaattgacc ttgccgctct aaaggagata aaagaggtta agcgccagag acttacggag
    12541 tacaaaaagt acagaccgaa ttccgaatac catgagggca ggggcgtttg gagcgtaaag
    12601 gctgatatcg cgctcccctg cgcaactcag aacgagctta acttagagga tgcaaaaatg
    12661 ctcgtgaata acggcgtttt cgcagtggcg gagggcgcta atatgcccac aaccgcagag
    12721 gcagtggatt tcctccttga aaacggcgta tacttcctgc ccggcaaggc tgcaaacgca
    12781 ggcggcgttg ccacatccgg tcttgagatg tgccagaaca gcggcaggct gagctggtcg
    12841 tttgaaaagg ttgatgaaaa ccttaaaaag attatgattg acatttacca caacatagat
    12901 aacgcggcta agaaatacgg ccttgagggc aactatgtag cgggcgcgaa tatcgcgggc
    12961 tttgaaaagg ttgcaaacgc aatgattgct caaggcgcgg tataaaaaca agttaataaa
    13021 ggcaaaggcg tctttaatgt taaaagcatt aaaggcgcct ctctcttttt ataaagtcac
    13081 taatgaaatt actatagaaa aggggtaaag attatgaaaa cggttaaaga ttattttggg
    13141 tccctggttt ttgacgacag agtaatgaag gaaatgctgt cagcggatgt ttacgcaaag
    13201 ctcaaaaaga ccatggacga aggcagcgag cttgacatca gcgtagccaa cgctgttgct
    13261 gatgcgatga aggaatgggc gatctcgaaa ggcgccaccc actttaccca ttggttccag
    13321 cctttaacag gtattacagc tgagaaacac gacagcttta tagctcccaa tccccacggc
    13381 ggagttatta tggagttttc gggcaaggag cttattaagg gcgagcccga cgcatccagc
    13441 ttccccagcg gcggacttcg cgccaccttt gaggcaagag gctacaccgc atgggatccc
    13501 acatcctacg cgttcattaa gggaaagacc ctttgcatac ccacagcttt ctgctcatac
    13561 gaaggtcacg ctctggataa aaagactccg ctgctgcgct ccatggaggc tcttaacagg
    13621 caggctttga gaatcctccg tctgttcgga aacacctctg caaagcatgt ccgcgcatgc
    13681 gtaggtcccg agcaggagta cttccttgtc acaaaagaga tgtacgacaa gagacctgac
    13741 ctgcgcttta cgggacgcac cctgttcgga gcaagacccc cgaagggtca ggagatggat
    13801 gaccattatt tcggagccat caagccccgt gtagccgcct ttatggagga gcttaacgag
    13861 gagctttgga aggtgggtat tctcgctaaa accgagcaca acgaggttgc tcccgcacag
    13921 cacgagattg ctcccattta cacaaccgcc aacatagcga cagaccacaa ccagctcaca
    13981 atggagataa tgcagaaggt tgccttaaag cacggacttg tatgcttgct gcatgaaaag
    14041 ccctttgcgg gcgtaaacgg cagcggcaag cacaacaact ggtccatcgc aaccgacagc
    14101 ggtcagaacc ttctctcccc cggcgacaca ccctatgaga acgcccagtt cctgctgttc
    14161 ctctgcgcag tcattaaggc agtggacgat tatcaggatt tgctcagaat ctccgtcgcc
    14221 accgcgggca acgaccacag actcggagct aacgaggctc cccccgctgt tgtttcaatc
    14281 ttcctcggcg atgagctgac cgctattctt gacgctatcg agagcggaac accgtataac
    14341 ggcacctcga agattgagat gaagctgggc gtcgatttcc tgcccaaatt ccatcgcgac
    14401 acaaccgaca gaaacaggac ctcgcccttt gcctttacag gcaataagtt cgagttcaga
    14461 atgctgggct cctcaaacag catagcctgc gcaaacatca tgctcaacag cgctgtcgcc
    14521 gagtccctct gtcagttcgc agacaggctc gagtcggcag gcaactttga ggcagagctc
    14581 aacagcctta ttaaagatac aatcaaggct cacaagagaa ttatctt
//
LOCUS       DAONVQ010000036        14355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3205_length_14355_cov_4.485385, whole genome shotgun sequence.
ACCESSION   DAONVQ010000036 DAONVQ010000000
VERSION     DAONVQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14355
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3205_length_14355_cov_4.485385"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PLD98_03665"
     CDS             <1..683
                     /locus_tag="PLD98_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05379.1"
                     /translation="EAAYRREMSDKYQNARRNYRDTVKKLEAQGVKFFNLCGYGLPLI
                     DFIESKNESSDAVINISSTSIGATSAPVGETFPDGYTPANPDIENAEQFISPDRTIDA
                     TTCLLPERTWFFRNQVHDDIAYNDVALEIAKKILSEDEFDSVFSDPRYPQFNGSRNIR
                     KIKYNLMPKAKSADRSKLSPEQVQALDEAIAYTEAMLANTIIVDNSEAKKAEEKLQAA
                     LAPLEEKK"
     gene            757..1473
                     /locus_tag="PLD98_03670"
     CDS             757..1473
                     /locus_tag="PLD98_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05380.1"
                     /translation="MANDVIIKVRNLSKSYGKNLVLDDISFDLEKGKIIGLLGPNGSG
                     KTTLIKILSGLIHDYDGIVRIDDNPTGIHTKAMTAYLPEKTYLADWFRPIDAINYFND
                     FFSDFDKAKALEFVKRFKLNPKQPIKSMSKGMQEKVQLLLIMSRSAKLYLLDEPLGGV
                     DPAARATILDIIMKNYAENSTLLLSTHMVNDLEGSFNHVLMLGHGKVLVNTSIEKIHS
                     MGKSVEEVFKEVIGNAWETD"
     gene            1454..2275
                     /locus_tag="PLD98_03675"
     CDS             1454..2275
                     /locus_tag="PLD98_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05381.1"
                     /translation="MLGKLIKNDLKFGAHSVSLVYLSALIASAVMGISLLADLERVKY
                     FSSIAFIICAFAIVIVTIITIIQSVSRTMYGNQGYLTHTLPVKTGALVFSKWFSAFFW
                     VFLSLLFLYASIVFMYFYISGESAFQMIKSILLSDELSMLINEKVLRDVAIIEAVRLV
                     IMVGVISMYVIFGITLSNVRPFSALGNIGTVIYSFITIVGGHYLGKGAAKLADLNIGV
                     TPDSLKLIATEAAKDAILYENGAIRPLTATFTEIVFAIILYFAVILLIERKINLK"
     gene            2298..2897
                     /locus_tag="PLD98_03680"
     CDS             2298..2897
                     /locus_tag="PLD98_03680"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HQA05382.1"
                     /translation="MNIIDISRDILDCEIYPGDPEPRVDIIRRIADGSECNLAALYTG
                     LHCGTHVDAPLHFIDKGMPIDMLPLDAFIGECTVISVPSGPITGEYVEKYFPRSKERI
                     LIKGRGRAYFHESAATELSLAGVKLVGTDSLSVGTKGQQTAVHKAFLREAVALLEGLN
                     LEEVEDGDYFLIAPPLKIGGVEASPVRALLISDYIFWSA"
     gene            2986..4179
                     /locus_tag="PLD98_03685"
     CDS             2986..4179
                     /locus_tag="PLD98_03685"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQA05383.1"
                     /translation="MSVLNKKTIEDLDVKGKRVLVRCDFNVPIQDGKITSDKRIQESL
                     PTIKYLIENGARVILCSHLGRPKGGEFEAEFSLAPVAARLSELLGKEVKMAKDVVGES
                     AQALSKELQDGDVMLIENVRFEKGETKNDPELSKKFAALADIFVNDAFGSAHRAHSST
                     AGVADYLPSAVGYLIQKEISFMGQALEAPKRPLVAILGGAKVSDKIGVINNLLEKVDT
                     LIVGGGMAYTFFAAKGYKVGTSICETDKIDLANEMLEKAKARGVKFLLPVDNKVGKEY
                     KPDTESMIVDSDKIPDDYMGLDIGPKTAELFAEAIKGAGTIIWNGPMGVSEWENFAHG
                     TIAVAKAVAESGAISIIGGGDSAAAVQKLGFADKMSHISTGGGASLEFLEGKVLPGID
                     CIDTK"
     gene            4320..5087
                     /gene="tpiA"
                     /locus_tag="PLD98_03690"
     CDS             4320..5087
                     /gene="tpiA"
                     /locus_tag="PLD98_03690"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQA05384.1"
                     /translation="MNKSLRPAVIAGNWKMNKTPDEAVTLIKQLIPLVKDADCEVVAC
                     VPFVDLHAAIEAAKGTNIKIGAQNCHWEKSGAFTGEVSAEMLKAVGAEYVIIGHSERR
                     TLFGDNDLTVQKRTRAALNAGLKVILCVGEYLEQREQGITTEVVRIQTKIALLGVAKE
                     ELKNIIIAYEPVWAIGTGKTATAEQANEVNKAIRDLIAELYGKDAADAMVIQYGGSMN
                     AGNAEELLAQPDVDGGLIGGASLKAEDFSKIVAAASK"
     gene            5117..6628
                     /gene="gpmI"
                     /locus_tag="PLD98_03695"
     CDS             5117..6628
                     /gene="gpmI"
                     /locus_tag="PLD98_03695"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQA05385.1"
                     /translation="MKKPYVLCIMDGFGENPEREGNAIKLASTPRLDEIFSEYPTTFI
                     GASGMDVGLPDGQMGNSEVGHTNIGAGRVVYQDLTRITKSIMDGDFFENETFLNAIEH
                     ARKNGGALHLMGLLSDGGVHSHNTHLYALLELAKKKNFDNVYIHCIMDGRDVSPTSGV
                     GFVSELFHKTKEIGVGTIATIMGRYYGMDREKKWDRVEKAYNAITKCEGVRFSCGKEL
                     MEKSYADGVTDEFVIPSVSDKAQPLKDGDSVIFFNFRPDRARQLTRAIVDPDFSGFER
                     EMLKDIYYVCMTQYDATMPNVEVAFKPDRIECPIGAVVSRHGMKQLRIAEYTKYAHVT
                     FFLNGGEERVYEGEDRILIDSPNVPTYDLKPEMSAYEVADTVTEKIRSGEYDFVVINF
                     ANCDMVGHSGVLEAAIKAVEAVDACVGKVYDTVMDMDGVMILTADHGNAEKMFDTDGS
                     PFTAHTTNPVPFTVIGYNCQLKEGGRLCDISPTMLELMGLEKPASMTGQSLIK"
     gene            complement(6932..7894)
                     /locus_tag="PLD98_03700"
     CDS             complement(6932..7894)
                     /locus_tag="PLD98_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HQA05386.1"
                     /translation="MLDSLFSFREHRMIQRLLDALQSALRPTGEGLGYFKSIIASLVV
                     VAELLGALFFGHPQTPRGQVINLDEWELVWRDEFDGDSLDLTKWRSNGNGPRRGGYWD
                     IDQAIVQDGNLIIRTEYKEDGKYGPGWYSAGLDTRGLFEKKYGYFEVSCKCPPGVGLW
                     GAFWTMSDGMSNPPDGSAADGCEIDIFESFNYNQGARHDAISHAVHYDGYGDGHTSVL
                     VGVYKGKNIYTEYNTYGLEWNENELIFYINGVETDRLSGVWVPQVAEYLLLSVEVAGT
                     VETINGQRVPVLAKDNNITMNPPSAFPIDFVVDYVRVYERKPAA"
     gene            complement(8708..9466)
                     /locus_tag="PLD98_03705"
     CDS             complement(8708..9466)
                     /locus_tag="PLD98_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05387.1"
                     /translation="MDDIFEMTKRAEERVKRFQERAKIFAEKLNEEFYEEEEQRPSKS
                     ELLPLPVNRSKSYNATEDIFQYLASRSRADFEETEREEESEETSKAVSGSPERRDERE
                     MNNSGYDTSTVHVSVPSRSSAWRASDYIDKSKKHHISMPVEIPERYRYVIPSKKKEEI
                     DYKELVRKQRDEPRLKLSLDSPPKKREPEERHEHHHHEHAEKSAGISKLAKALNLDES
                     ALDTDELLLLTLILLLAEEGGDMPLLIALMYILI"
     gene            complement(9482..9880)
                     /locus_tag="PLD98_03710"
     CDS             complement(9482..9880)
                     /locus_tag="PLD98_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05388.1"
                     /translation="MDINELLSSLSPDDIEKLKETAASLLGDNEEKAQSVASILDKRK
                     EDTKELSVKEDDAQMVNALVSRLGSNKAMPTLLKAMGELEKNMNKRDARSDFLFALKP
                     LLKKHRQSKVDEAVKIMRLFSLLPLLKGER"
     gene            complement(9924..10136)
                     /locus_tag="PLD98_03715"
     CDS             complement(9924..10136)
                     /locus_tag="PLD98_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05389.1"
                     /translation="MKLRGSDDQINELISTLKSYDKDKLSKFIDRNLDSRQKKRLEEV
                     LSNDKKLNEMLNSELGRELRKKIEDM"
     gene            10325..10687
                     /locus_tag="PLD98_03720"
     CDS             10325..10687
                     /locus_tag="PLD98_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HQA05390.1"
                     /translation="MAKNFEISVLLDFYGDMLTQKQREFLEDYYNHDLSLSEIAENVG
                     ITRQGVRDSIKRAETQLYKMEEMLGLVKRFEQIRAGLEEIIACASEINKYNLNNGLSR
                     EINDYTVRIKSIATALCE"
     gene            10721..12073
                     /gene="ffh"
                     /locus_tag="PLD98_03725"
     CDS             10721..12073
                     /gene="ffh"
                     /locus_tag="PLD98_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQA05391.1"
                     /translation="MAFEGLSEKLEAAFKKLRSKGTLTESDVRTAMREVRLALLEADV
                     SYKVAKDFTAAVTERAIGSKVMESLTPAQMVVKIVYEELTALMGGTQARLTYSPRPPT
                     VVMMCGLQGSGKTTHSAKIAMRLKNQGHHPMLVACDIYRPAAIKQLQVVGEQAGVPVF
                     EMGTENPVTIAKEAVKHAKDMGYDYVFLDTAGRLHIDEALMNELKAIKAEVKPHEILL
                     VVDAMTGQDAVNVAASFNEALGIDGLVLTKLDGDTRGGAALSARAVTGKPIKFVGLGE
                     KLDALDVFYPDRMASRILGMGDVLSLIEKAEMALDEKKAKELEENLRKNKFDLEDLLT
                     QLQEMKKMGSVKDILMMIPGIGNKIKDTDIDDSHLRKTEAIILSMTPEERRKPDIINP
                     SRKRRIAAGCGLKVEDVNRVLNQHRQMQKMFKSLNGKVGAKKMRRLGLTGSGLEQFLK
                     "
     gene            12122..12364
                     /gene="rpsP"
                     /locus_tag="PLD98_03730"
     CDS             12122..12364
                     /gene="rpsP"
                     /locus_tag="PLD98_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQA05392.1"
                     /translation="MAVRIRLRRMGAKKAPFYRVIVADSRSPRDGRFIEEIGYYNPMT
                     APAEIKIDLEKAEKWIKNGAQPTDTVKALLKKVKPE"
     gene            12446..12679
                     /locus_tag="PLD98_03735"
     CDS             12446..12679
                     /locus_tag="PLD98_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HQA05393.1"
                     /translation="MNELLITMAKGLVEDPDAVSVTVDEPNEDGVVVYHLHVAPDDMG
                     RVIGKQGRIAKALRMIMRATANRRDMKVQVEID"
     gene            12812..13324
                     /gene="rimM"
                     /locus_tag="PLD98_03740"
     CDS             12812..13324
                     /gene="rimM"
                     /locus_tag="PLD98_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060024.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQA05394.1"
                     /translation="MLKKYLEIGQIVGTHGIKGEVRINPWCDSPEFLTGFKKLYKDEG
                     RSHFEVTSLRVHHNIVLGKFKGIDTVNDAAKLRDTVLFIDRDDAEIPEGSYFIQDLIG
                     CAVTDVDTGENLGVLSDVSKTGANDVWHIKRGNKEYLIPAIDEVVIETDVENKSIKIR
                     PLKGIFDDED"
     gene            13314..14066
                     /gene="trmD"
                     /locus_tag="PLD98_03745"
     CDS             13314..14066
                     /gene="trmD"
                     /locus_tag="PLD98_03745"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQA05395.1"
                     /translation="MRIDILTLFPEMCERVFTESITGRAIKNGYIDINAVDIRKYAEP
                     QRKGRVDDAPYGGGEGMIMQAEPIARCFEDIAGNSPEKPYFIYMSPAGQTLTQDKVKE
                     LSERKHLVLLCGHYEGVDERVIEELVDEEISIGDYVLTGGELPALVLADAVARMIDGV
                     LSSKEAVEGESHYNYLLEHPQYTRPPVWRGRQVPEVLLSGHHANIKKWQREQSLIRTY
                     KRRPDLFKKAEVSDKEKEFVENFIAEENGEKD"
BASE COUNT         3701 a         3644 c         3650 g         3360 t
ORIGIN      
        1 aggaggccgc atatcgccgt gagatgtccg acaagtacca gaacgcaagg cgcaactaca
       61 gggataccgt aaagaagctg gaggctcagg gcgtaaagtt cttcaacctc tgcggctacg
      121 gtctgccgct gattgacttc atcgagtcaa agaatgaaag ctccgacgcc gttatcaaca
      181 tctcctcaac atctatcggc gcgacctcag ctcccgtggg cgagaccttc cccgatggct
      241 acacccccgc aaatcccgat atagagaacg ctgagcagtt catctccccc gacaggacca
      301 tagacgccac aacatgcctt ctccccgaga ggacctggtt cttcagaaat caggttcacg
      361 acgatatcgc ctacaacgat gtggctcttg agattgccaa gaagattctc agcgaggatg
      421 agttcgacag cgtcttttcg gaccccagat atcctcagtt caacggctca aggaatatca
      481 ggaagattaa gtacaacctt atgccgaagg ctaagtcagc cgaccgctca aagctcagcc
      541 ccgagcaggt tcaggctctt gacgaggcaa ttgcatatac agaggctatg cttgccaata
      601 ctataattgt cgacaactcc gaggcaaaga aagcagagga aaagctgcag gccgcgcttg
      661 ctcctttgga ggaaaagaaa taatcaataa aggggcatag gcaaagccct atgccccgta
      721 ctgtcacgcg gcggcagccg tttttggagg ctttgtatgg caaacgatgt aatcattaaa
      781 gtccgcaacc tttcaaagtc atacggcaaa aaccttgttc ttgacgatat atcctttgac
      841 cttgaaaagg gcaaaatcat aggacttttg ggacccaacg gcagcggcaa aacgacgctg
      901 attaagatac tttcgggtct tattcacgac tatgacggca ttgtaaggat tgacgacaat
      961 ccgacgggaa tccacacaaa ggcaatgacc gcctatctgc ccgaaaagac ctatctggcg
     1021 gactggttca ggcccattga cgccataaat tattttaacg atttcttctc cgatttcgac
     1081 aaggcaaagg cccttgagtt cgtaaagcgt tttaagctga atccgaagca gcccatcaaa
     1141 tccatgtcaa agggtatgca ggagaaggtt cagctccttc tgataatgtc aaggtccgca
     1201 aagctctatc ttctggacga gcctttgggc ggcgtggacc ctgccgcaag ggcgaccata
     1261 cttgacatta taatgaaaaa ctacgcggaa aactcaaccc tactgctctc aactcacatg
     1321 gtgaacgacc ttgagggcag ctttaaccat gttctgatgc tgggtcacgg caaggtgctg
     1381 gtcaacacaa gcattgaaaa gattcactcc atgggcaagt cggttgagga agtatttaag
     1441 gaggtcatag gcaatgcttg ggaaactgat taaaaacgac cttaaattcg gcgcccacag
     1501 cgtatccctt gtctatctct ccgcccttat cgcctccgcg gttatgggca tttccctgct
     1561 tgccgacctg gagagagtaa agtacttctc gtcaatcgcc tttattatct gcgcctttgc
     1621 cattgttata gtgacgataa ttactataat ccagagcgtt tcaaggacca tgtacggaaa
     1681 tcaagggtac cttacccata ccctgcccgt taaaacggga gcccttgttt tttcaaagtg
     1741 gttctccgcg ttcttctggg tatttttaag ccttctgttc ctttacgcct ccatcgtctt
     1801 tatgtacttc tacatctccg gtgagagcgc gttccagatg ataaagtcca tccttctctc
     1861 cgatgagctg agcatgctca taaacgagaa ggttttaagg gatgtcgcga tcatagaggc
     1921 tgtaaggctt gttattatgg tcggagtcat ttcaatgtat gtgattttcg gcattaccct
     1981 ttcgaatgtc cgcccgtttt cggctctcgg aaatatcgga acggtcatct attcctttat
     2041 aaccattgtc ggcggacatt atttgggcaa gggcgcggct aaactcgccg acctcaacat
     2101 cggcgtaact ccggacagct taaagctgat tgccaccgag gcggcaaagg acgcgattct
     2161 ttacgaaaac ggagctatcc gcccccttac cgccacattt acggagattg ttttcgctat
     2221 aattctttat tttgcggtta tccttttaat tgaaagaaag ataaatctga aataagccga
     2281 aaaaaggggt gcagagcatg aatataatcg atatttcaag agatattctt gactgtgaga
     2341 tttaccccgg tgaccctgag ccaagggttg acatcatacg caggattgcc gacggctcgg
     2401 agtgcaatct tgccgccctt tacacggggc ttcactgcgg aactcatgtg gacgctcccc
     2461 ttcacttcat agacaaggga atgccaatag atatgctccc ccttgacgct tttatcggcg
     2521 agtgcacggt gatatccgtt ccgtcgggcc cgattacggg cgagtatgtt gaaaagtact
     2581 tcccccgctc caaagagaga attcttatca aaggcagagg cagagcctac ttccacgaaa
     2641 gcgccgctac ggagctttcc ctggcgggcg taaagctggt tggaaccgac tcattgtcgg
     2701 tgggcacaaa gggtcagcag accgccgttc acaaggcgtt tttgagggag gcggtggccc
     2761 tcttggaagg attgaatctt gaagaagtgg aagacggcga ctacttcctt atcgctcctc
     2821 ctttaaaaat cggcggagtt gaggcgtcgc ccgtcagagc cctgcttatt tccgactaca
     2881 tcttctggag cgcgtaacaa cggcatttgg tgcgaaagca cttaccgcgt atgcggatat
     2941 atggtattaa ctcttttcta tattttaatt gtaaggagaa tttgcatgag cgtattgaac
     3001 aaaaagacca ttgaggattt ggatgttaag ggcaaacgag ttcttgtccg ctgcgacttc
     3061 aatgtgccga ttcaggacgg caaaatcacc agcgataaga gaattcagga atccctgccc
     3121 acaatcaagt atcttataga aaacggcgca agggttatcc tttgctccca tctgggcaga
     3181 ccgaagggcg gcgagtttga ggctgagttc tcccttgctc ccgtagcggc ccgcctctcc
     3241 gagcttttgg gcaaagaggt taagatggct aaggatgtcg taggcgagtc cgcgcaggct
     3301 ctctcaaaag agcttcagga cggcgatgta atgcttattg aaaatgtccg cttcgaaaag
     3361 ggcgagacaa agaacgaccc cgagctcagc aagaagtttg cggctctcgc agacatcttt
     3421 gttaacgacg ctttcggctc cgcgcacaga gcccactcct ccacagcagg tgtagcagac
     3481 tatctgccct ccgctgtcgg ctacctcatc cagaaggaaa tctccttcat gggacaggct
     3541 cttgaggctc ccaagcgtcc cttagtcgca attctcggcg gcgcaaaggt ttccgacaag
     3601 ataggcgtca tcaacaacct gcttgaaaag gttgacaccc ttatcgtagg cggcggcatg
     3661 gcatacacct tctttgccgc caagggctac aaggttggca catccatctg cgagaccgac
     3721 aagattgacc ttgcgaacga gatgctggaa aaggcaaagg caaggggcgt taagttcctc
     3781 cttcccgttg acaacaaggt gggcaaagag tacaagcccg acacagagtc catgatagtt
     3841 gactccgaca agattccgga tgactacatg ggacttgaca tcggacccaa gaccgccgag
     3901 cttttcgcag aggctatcaa gggcgccggc acaatcatct ggaacggccc catgggcgtt
     3961 tccgagtggg agaacttcgc tcatggcaca atagctgtcg ctaaggcagt ggcagagtcg
     4021 ggagccatct ccatcatcgg cggcggcgac tcggcagcgg cagttcagaa gctgggattc
     4081 gccgataaga tgtcacacat ctcaacaggc ggcggcgcat cgctggagtt ccttgagggc
     4141 aaggtacttc ccggcatcga ctgcatagac acaaaataaa tcgaaagagt ctgcatattg
     4201 aaaaatatgc agacatattt catatgcaca aatttccgcc cgaaattttg tgatattttt
     4261 actgacaatt ctctcagagc gaaatataat aaaaacatcc gataaaaagg agattatata
     4321 tgaacaaaag ccttcgtccc gctgttatcg cgggcaactg gaaaatgaac aagactcccg
     4381 atgaggcggt cacacttata aagcagctta ttccccttgt aaaggacgcg gactgcgaag
     4441 tggtcgcctg cgttcccttt gtggaccttc acgccgcaat tgaggcggct aagggaacca
     4501 atataaagat aggcgctcag aactgccact gggaaaagag cggagctttt acgggcgagg
     4561 tttcagccga aatgctcaaa gccgtcggag cggagtatgt tatcatcggt cactccgagc
     4621 gcaggacact tttcggcgac aacgacctga cggttcagaa aagaacaagg gcggcactca
     4681 acgccggtct taaggtcatc ctctgcgtgg gcgagtatct tgagcagagg gagcagggca
     4741 ttaccacgga ggttgttcgt attcagacca agattgccct tttgggagta gcgaaagaag
     4801 agcttaaaaa catcatcata gcctatgagc ccgtttgggc aataggcacc ggcaagaccg
     4861 cgacagccga gcaggctaac gaagtcaaca aggctataag agaccttatc gccgagcttt
     4921 acggcaagga cgcggcggac gccatggtca tccagtacgg cggctcaatg aacgcgggca
     4981 atgccgagga acttctcgct cagcccgatg ttgacggcgg acttatcggc ggcgcttcct
     5041 taaaggccga ggacttctca aaaatcgtcg cggccgcaag caaataagcc aaactcatat
     5101 tgttaaggag taaaccatga aaaaacctta tgttctttgc atcatggacg gattcggcga
     5161 gaaccccgaa agggaaggca acgccataaa actcgccagc acaccccgtc ttgatgagat
     5221 attctcagaa taccccacca cattcatagg cgcgtcgggc atggatgtgg gtctccccga
     5281 cggtcagatg ggcaacagcg aggtcggtca cacaaatatc ggcgccggca gagttgtcta
     5341 tcaggatttg accagaatca caaagtccat catggacggc gatttctttg agaacgagac
     5401 cttccttaac gccatagagc acgcaaggaa gaacggcgga gctttgcatc tgatgggtct
     5461 tttgtccgac ggcggagttc acagccacaa cacccatctt tacgcccttt tggagcttgc
     5521 aaagaaaaag aattttgaca atgtctacat ccactgcatt atggacggca gagatgtctc
     5581 acccacatcg ggcgtaggct ttgtttccga gctgttccat aagacaaagg aaatcggcgt
     5641 tggcaccatt gccaccatta tgggcagata ctacggcatg gaccgcgaga agaagtggga
     5701 cagagtcgag aaggcttaca acgccatcac caagtgcgag ggcgtgcgtt ttagctgcgg
     5761 caaggagctt atggagaaat cctatgccga cggcgtaacg gacgagtttg tcattccttc
     5821 cgtttccgac aaggctcagc ccttaaagga cggcgacagc gtaatattct ttaacttccg
     5881 tcccgacaga gcaagacagc tgacaagagc catagtcgac cccgacttca gcggctttga
     5941 aagggagatg ctgaaggaca tttactatgt ctgcatgacc cagtacgacg ccacaatgcc
     6001 caatgttgag gtagccttca agcccgacag aatcgagtgc cccatcggcg ctgttgtaag
     6061 ccggcacggc atgaagcagc tcagaatcgc cgaatacaca aaatacgctc atgttacatt
     6121 cttcttaaac ggcggcgagg aaagggttta cgagggcgag gacagaatcc tcatcgactc
     6181 ccccaatgtt cccacctatg acttaaagcc cgaaatgagc gcttatgagg ttgccgacac
     6241 cgtcaccgag aaaatcagaa gcggagagta cgactttgtg gtcatcaact tcgcaaactg
     6301 cgacatggta ggtcactcgg gcgttcttga ggcggcaatc aaggccgttg aggctgttga
     6361 cgcctgcgtg ggcaaggttt acgacacggt tatggacatg gacggagtta tgatacttac
     6421 cgccgaccac ggcaacgccg aaaagatgtt tgacaccgac ggctcaccct tcaccgccca
     6481 caccacaaac cccgtaccct ttacggttat aggctataac tgccagctta aagaaggcgg
     6541 cagactctgc gacatctccc ccacgatgtt agagcttatg ggtcttgaaa agcccgcctc
     6601 catgacagga cagagcctta taaaataaat ttaagccctc cggacggagg gctttattat
     6661 tagcgtttcg cccaaattta aatggacggc gcatgtaaaa atcacgacat tatcgggaag
     6721 gctttcgccc ttttaaatca tttcggcgta ctttctgttt tcctttttag agcaatcggt
     6781 tatccccaga aaatccgcgt cacgccttag ccgcgccaat tttccgaaga acgagtctat
     6841 ctctctccat aaaccgcatc ttttataaaa ttcacgcaag gaattttgtc cgatcgcaat
     6901 aataaagccc ctgcctggca ggggcgattt tttatgctgc gggttttctt tcgtagactc
     6961 ttacataatc gaccacaaag tcaatgggga aagcgctcgg gggattcatg gtaatgttat
     7021 tgtcctttgc gagaacgggc accctttgac cgtttatggt ctccacagtg cctgcaacct
     7081 caacggagag aagcagatac tcggcaacct gaggtaccca tacgcccgag agcctgtcgg
     7141 tctcaactcc gttgatgtag aaaatcagct cgttctcgtt ccactcaagt ccgtatgtat
     7201 tgtactcggt gtatatattc ttgcccttgt aaacgccaac cagaacggag gtatgaccgt
     7261 cgccgtagcc gtcatagtga accgcgtggc tgattgcgtc gtgcctcgct ccctgattgt
     7321 agttgaagga ctcgaaaata tcgatttcgc agccgtcagc ggctgagccg tcggggggat
     7381 tggacatgcc gtcgctcatt gtccagaaag cgccccaaag acctacgccg ggagggcatt
     7441 tgcagcttac ttcaaagtag ccgtacttct tctcaaacag tcctcttgtg tcaagacctg
     7501 cggaatacca gccgggaccg tatttgccgt cctctttata ctcggtgcgg ataattaaat
     7561 tgccgtcctg tactattgcc tggtctatgt cccagtagcc gccccttctc gggccgttgc
     7621 cgttgctcct ccactttgta aggtcaaggc tgtcgccgtc aaactcgtcg cgccagacaa
     7681 gctcccactc gtcaagattt atgacctgtc cgcgtggggt ctggggatgg ccgaaaaaca
     7741 gcgcgccaag cagttccgcc acaactacaa gggaggcgat tatacttttg aaatagccga
     7801 gaccctctcc cgtgggtctg agagcgctct gcagagcgtc aagaagcctt tgaatcatcc
     7861 tgtgttctct aaaggaaaat agcgaatcca gcataatatc cctcctttaa tctttaattt
     7921 catattagcc agtaaattcc acaatgtcaa caaataaaaa acgcaaaatc aataaagttt
     7981 cagcaaagtg acaaaggcgg cgtttgggcg gtgacactcg ctgaggattg gcgcgcgggg
     8041 taaaaaatac ccctgcgtgc ggcggggcgc gagggcgacg actttactct ttgggcgggc
     8101 ggctccccct tctttttcac cccgccactt gcgggcgggg gtgtcggcga tgggcgtggg
     8161 gtatatataa aaagccccca tcggacggct gggcggcgtt aaccgaggtg ggcggcacac
     8221 cccatggcgg gggtaataat aaaaaccccg atggcgggtc tgctcccact gggcggcggg
     8281 acggaacgag cggctgatgg tcttttttta tggcagggcc gctccccgac gggcaggata
     8341 ggaacaaaaa aagccgcagc ggacggcggg ggcggcactt ctctaagagg gcggggcgca
     8401 accctcaaac tgacgacgga aatcccctgc ggatgagagc ggctgggcgg aaattaaaat
     8461 ccctcggcgg gcggggtgtc ggcgatgggc ggtggggtgc atataaaaaa gcccccgaag
     8521 gacggcgggc ggcgttaaac gagatggggc ggtgttacgc tccccatctg tgggcggctc
     8581 tacccgtgtc ggggcggcgg gcaaaattaa aaccctgccg tgtggcaggg tcttaaaata
     8641 gtcccccctt taagagtatt actctggatt aattcccccc tttattgttt agtgcggctg
     8701 ccctccttta tatcaaaata tacattagag caataagtaa aggcatatcg ccgccctcct
     8761 ccgcaaggag gagaatcaaa gtcaaaagca gcagctcgtc ggtgtcaagg gcgctttcgt
     8821 caaggtttaa cgcctttgcg agctttgaga tgccggcgct tttctccgcg tgttcatgat
     8881 gatggtgctc atgtcgctcc tcaggctccc ttttctttgg gggagaatcg agagagagct
     8941 ttagtctcgg ctcatcccgc tgctttctta ccagctcctt ataatctatc tcctctttct
     9001 ttttggaggg gattacatac ctgtaccttt cggggatttc cacaggcatt gatatgtggt
     9061 gctttttgct tttgtctatg taatccgacg ctctccacgc gctggacctt gagggcacgg
     9121 agacatgtac cgtgcttgtg tcgtagccac tgttattcat ctccctctca tctctccttt
     9181 cggggctgcc tgaaactgcc ttgctcgttt cctcgctttc ctcttccctt tctgtttcct
     9241 caaagtccgc tcttgacctg gaggcaagat actgaaatat atcctccgtc gcgttatagg
     9301 atttgctcct gtttacgggc aaaggcaaaa gctcggattt ggagggtctt tgctcttcct
     9361 cctcgtaaaa ctcctcattc agtttctccg caaaaatctt cgccctctcc tgaaagcgtt
     9421 ttaccctctc ctccgcccgt ttggtcatct caaaaatatc gtccactttt cagccccctt
     9481 tctatctttc gcctttaaga aggggcagca agctgaaaag acgcattatc tttaccgctt
     9541 cgtccacttt gctctgcctg tgcttcttta aaaggggctt tagagcaaag agaaaatcgg
     9601 acctggcgtc ccttttgttc atattctttt ccagttcgcc catagccttt aaaagcgtcg
     9661 gcatagcctt gttggacccg aggcgcgaca caagagcgtt taccatttgc gcgtcgtctt
     9721 cttttacgga cagctccttt gtgtcctctt tccttttgtc aaggatagac gccacgctct
     9781 gagccttttc ctcgttatct cccaaaaggg aggcggcggt ttctttcagc ttttctatgt
     9841 catcggggct tagcgatgat agcagctcgt ttatatccat gatttcactc ctttttatgt
     9901 ggatttcttt tcccttccgc tttttacata tcctctattt tctttctgag ctccctgccg
     9961 agctcgctgt tgagcatctc attcagcttt ttgtcgttgc ttaaaacctc ctccagcctc
    10021 tttttctggc gggaatcaag gtttctgtca ataaacttgg acagcttatc tttatcgtaa
    10081 gacttcagtg tggagataag ctcgtttatc tggtcgtccg agccgcgcag cttcataaac
    10141 tcaccctttt tctgctaaaa tcgatataaa aacacctcaa ttaccatata tatgcaaaat
    10201 tcttctacta ttgcttgtat ggcttttttt aaatttgcaa ttgacaatat cggcgcgatt
    10261 tagtataata cttacctgta aaggaaacag gctttacact atactgcttc aggagggctt
    10321 ttcggtggca aaaaactttg agatttccgt gcttcttgat ttttacggcg atatgctcac
    10381 ccaaaagcag cgcgaatttc ttgaagacta ttataatcac gacctctctc tttccgagat
    10441 tgcggaaaat gtggggataa caaggcaggg agtgcgcgac tccattaaaa gagcggaaac
    10501 ccagctttac aaaatggagg aaatgctggg actggtcaaa aggttcgagc agattcgcgc
    10561 cggtcttgag gagataatag cctgcgcgtc ggaaatcaat aaatacaatc ttaacaacgg
    10621 tctttcaaga gagattaacg actatacggt cagaataaaa tccatagcga ccgctctttg
    10681 cgaatagctt tggactttat taaaaataaa ggagaaatgt atggcatttg aaggtctttc
    10741 tgaaaaattg gaggccgcgt ttaaaaagct gaggagcaag ggcaccctta ccgagtccga
    10801 tgtcagaacc gccatgcgcg aagtgcgcct ggcgctcctt gaagcggatg tcagctataa
    10861 ggtggcaaag gatttcaccg ccgccgtcac cgaaagggca atcggctcaa aggtaatgga
    10921 aagccttact cccgcgcaga tggttgtaaa aatcgtctac gaggagctca cggctttaat
    10981 gggcggcaca caggcaagac tcacatactc cccccgaccg ccaacggtag ttatgatgtg
    11041 cggtctgcag ggttcgggca aaaccaccca cagcgcgaaa atcgcgatga ggcttaaaaa
    11101 tcagggtcat caccccatgc ttgtcgcctg cgatatttac aggcccgctg ctataaagca
    11161 gcttcaggtt gtgggcgaac aggcgggcgt gccggtcttt gagatgggca ccgaaaaccc
    11221 cgtcaccata gccaaagaag ccgtaaagca tgccaaagac atgggctatg actatgtgtt
    11281 ccttgatacg gcaggccgtc tgcacattga cgaggctctt atgaacgagc tgaaggcaat
    11341 aaaggcagag gtaaagcccc atgaaattct gcttgtggtc gacgccatga caggtcagga
    11401 cgccgtaaat gtggcagcct ccttcaatga ggctctgggc attgacggtc ttgtccttac
    11461 aaagctggac ggcgacacaa ggggcggcgc cgccctctcc gcaagagcgg taacgggcaa
    11521 gccgattaaa ttcgtcggtc tcggcgaaaa gctggacgcc cttgatgtct tctaccccga
    11581 ccgcatggca tcgagaattc tcggcatggg cgatgttctc tcccttatcg aaaaggctga
    11641 gatggctctt gacgagaaaa aggcaaagga gctggaggag aacctcagaa agaacaagtt
    11701 cgaccttgag gaccttctca ctcagcttca ggaaatgaaa aaaatgggct ccgtgaagga
    11761 tatactaatg atgatacccg gcatcggaaa caagattaaa gacaccgaca ttgacgacag
    11821 ccatctgaga aaaaccgagg ctatcattct gtcgatgact cccgaggaga gaagaaagcc
    11881 ggatattata aacccgtcac gcaaaaggcg catagccgcc ggctgcggac ttaaagttga
    11941 ggatgtaaac cgcgttctca accagcacag acagatgcaa aagatgttca agagcctgaa
    12001 cggcaaagtc ggcgccaaaa aaatgcgccg tctgggtctt acaggcagcg gtcttgagca
    12061 gtttttgaag taaaatggag actctcaaac taatctatac aaactttgga ggtaaatttt
    12121 aatggcagta agaatccgtc tgcgccgcat gggcgcaaag aaggcacctt tctatcgcgt
    12181 tatcgtcgct gattcccgtt caccccgtga cggcaggttc atagaggaaa tcggttacta
    12241 caatccgatg accgcccccg ccgagattaa gatagacctt gaaaaggccg aaaagtggat
    12301 aaagaacggc gctcagccca ccgatacagt taaggctctg cttaagaaag taaagcccga
    12361 ataaaggtgc ccacaaaaat cgcggtcaat aggcttagcc tttgccgaat aaattaccac
    12421 ttcgcatctt taggaggaaa agaaaatgaa tgaactgctg atcacaatgg caaaaggact
    12481 tgttgaggat cccgatgccg tctccgtcac cgtagatgag cccaacgaag acggcgttgt
    12541 agtttatcat ctgcatgtcg ctcccgatga catgggcaga gttataggca aacagggcag
    12601 aatcgctaag gctctgcgta tgattatgcg cgctaccgct aaccgcagag acatgaaggt
    12661 tcaggtggaa atcgactaat cggctttggc taaagcccaa aacccataca gtccgaagac
    12721 cgcagctttt taaggctgcg gtttttttaa tatttccata ttttcgattt atgctataat
    12781 atataggctc aaaaatattt tggagggcaa gatgcttaaa aaatatctgg aaatcggaca
    12841 aatagtcgga actcacggaa ttaagggcga ggtaagaata aatccctggt gcgacagccc
    12901 cgagtttctg acgggtttta aaaagcttta caaggatgag ggcaggtccc atttcgaggt
    12961 gacctctctc agagtgcatc acaatattgt ccttggaaag tttaagggca ttgatacggt
    13021 aaacgacgcg gcgaaactcc gcgacaccgt tttgttcatc gacagggatg acgccgaaat
    13081 ccccgagggc agctacttta ttcaggacct tataggctgc gctgtcaccg atgttgacac
    13141 gggcgaaaac ttaggcgttc tcagcgatgt atcaaagacg ggcgccaacg atgtatggca
    13201 cattaaaagg ggaaacaagg agtacctcat ccccgccatt gacgaggttg ttatcgaaac
    13261 cgatgttgaa aacaagagca tcaaaatccg tccgctgaaa ggtatatttg acgatgagga
    13321 ttgatatttt gaccctcttt cccgagatgt gcgaaagagt ttttacagag agcataacgg
    13381 gcagggcgat aaaaaacggc tatattgata ttaacgccgt tgacataaga aaatacgccg
    13441 agccgcagcg caaaggcaga gttgacgacg ctccctacgg cggcggcgag ggcatgatta
    13501 tgcaggcgga gcccatagcc cgctgctttg aggatatcgc gggaaactcc cccgaaaaac
    13561 cctattttat ctatatgtcc ccggccggac agactctgac ccaagacaag gttaaggagc
    13621 tctccgagcg gaaacacctc gtgctccttt gcggtcacta tgagggcgtt gacgaaaggg
    13681 tcatagagga gcttgtggac gaggaaattt caataggcga ctatgtcctg accggcggcg
    13741 agctgcccgc ccttgtgctt gccgacgcgg tggcgaggat gattgacggc gtgctctcaa
    13801 gcaaggaggc tgtggaaggg gagagccact ataattacct tctggagcat ccccagtaca
    13861 cccgcccccc tgtctggcgg ggcaggcagg tgcccgaagt gcttttgtcg ggtcaccacg
    13921 caaacataaa aaagtggcag cgagagcagt cgctgataag aacatacaag cgccgccccg
    13981 acctttttaa gaaggccgaa gtttccgaca aggaaaagga atttgtcgaa aactttattg
    14041 ctgaggagaa cggtgaaaag gattaattgc agtaaaaaat attaaccaaa gaaaaaagca
    14101 ctcctaaaga gtgctttttt attgtttgcg caaggaaagg ctgttgttcg gtcggccgga
    14161 atcagcccaa aactccccgc ccgcagaaag ctcccccgcc cgcacgggtc aggggcagga
    14221 aggcactccc atggcagcgg ggcaaagtcc ccccccacgg cggcgggcgg agagggcggg
    14281 cgcaccccca cgggggtgag gcaaaatcct cccaacgatg gcgggaaggg ataaggcggc
    14341 ggggcaaaat cctcc
//
LOCUS       DAONVQ010000037        14308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3222_length_14308_cov_4.626324, whole genome shotgun sequence.
ACCESSION   DAONVQ010000037 DAONVQ010000000
VERSION     DAONVQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14308
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3222_length_14308_cov_4.626324"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLD98_03750"
     CDS             complement(<1..312)
                     /locus_tag="PLD98_03750"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HQA05396.1"
                     /translation="MKTVIDYVLYFFFYSALGWLVESIYCSVGEKKWINRGFLTGPIC
                     PIYGTGAVVLSVCLTPFKDKWYLVFIIGLILADIVEFITSVIMEKLFHARWWDYSHYR
                     FN"
     gene            complement(309..854)
                     /locus_tag="PLD98_03755"
     CDS             complement(309..854)
                     /locus_tag="PLD98_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQA05397.1"
                     /translation="MGFLYDAYIRSYSLNTEDDADFFQYIDDLYFREELQGMSVYPQH
                     GDINRVQHILSVAYLSYVLAKKYNARVKEVCRGAILHDLFYYDWHVGEKGHRLHGYRH
                     PGFALKNARALTVLNKIEEDIIKRHMWPLTPTPPRYKESYIVTLADKYCAWQEILICR
                     IKSHREKFQKNLNTLNKVREL"
     gene            complement(892..1725)
                     /locus_tag="PLD98_03760"
     CDS             complement(892..1725)
                     /locus_tag="PLD98_03760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQA05398.1"
                     /translation="MKKTVYISDLDGTLLTSDAEISAKSAEIINRLISKGMLFTVATA
                     RSVSSSKRILSCLDLNLPVVLMNGVFIADIKSGRVIESVPIEKNTALKIIEIFKSFNV
                     SPFMYCAEGDMLDVVFEKIDSKQNEMFYKRREKQYYRRFEQVEKLTPPDNGECVYFNI
                     VDSYSRLKPLADKLRSVKGVSVAFYEDTYLEDSWFLEVFSSKASKCSGMLRIKEMTGA
                     ERAVVFGDNLNDISMFECADESFAVSEAVPQLKKIATGVIGSHDEDAVAKHLEKIFEY
                     R"
     gene            2286..4019
                     /gene="aspS"
                     /locus_tag="PLD98_03765"
     CDS             2286..4019
                     /gene="aspS"
                     /locus_tag="PLD98_03765"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQA05399.1"
                     /translation="MSKYRTHNCGELRTEHIGQNVKLAGWVDTVRDHGGVIFLDLRDH
                     YGVTQTVFHDETMLEGISRESVISVEGKVVKRDEETVNNKIATGQVEVVVDKIEVLGP
                     AIARLPFIPGESTATREDVRLKYRFLDLRNPEMHDVIVLRSKVISFLRKKMEELGFVE
                     IQTPILTCSSPEGARDYLVPSRKHKGKFYALPQAPQQFKQLLMASGFDKYFQIAPCFR
                     DEDARADRSPGEFYQLDFEMAFAEQEDVLKVAETVLYETFTAFSDFPVSKPPFKRIPY
                     AEAMLTYGTDKPDLRNPLIIKDLTDLFADVDFPAFKGRPVRGIKADCTGKSRSFFENM
                     LKFALSIGMKGLGYLTLAGDTFKGPIDKFLTPEKKAELIRIVDLKDGETVFFICDSLP
                     IVNTYAGQIRTELCNQLGLLDKDRFEMCFITDFPMYEINEETGKIDFTHNPFSMPQGG
                     LEALNNQDPLTILAYQYDTVCNGVELSSGAVRNHHPEIMQKAFEIAGYSHEELKAKFG
                     ALYTAFTYGAPPHAGMAPGVDRMIMLLAGKNNIRDVIAFPLNSNAQDLLLGAPNEVTE
                     KQLREVHIKLR"
     gene            4053..4355
                     /locus_tag="PLD98_03770"
     CDS             4053..4355
                     /locus_tag="PLD98_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     C"
                     /protein_id="HQA05400.1"
                     /translation="MVSVDKIKRIANLAKIHISEEEANQLAEQMSSIIAFADEINNSS
                     LPVSEEELINEYDRTEYREDEVRPSSDINDILLNSGGTQGNFFMVKDTAKAGRRYG"
     gene            4348..5802
                     /gene="gatA"
                     /locus_tag="PLD98_03775"
     CDS             4348..5802
                     /gene="gatA"
                     /locus_tag="PLD98_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HQA05401.1"
                     /translation="MVNISYYKKLLDTKALSCTELTKAFLDSAEKENPSLNAYISITK
                     EEALKTAAEVDEKIARGESLQPLEGIPVIIKDNICTKGIRTTCASKMLENYVPFYDAT
                     VWEHIKAQNSVLIGKGNMDEFAMGSTSETSYFGIVKNPHNPEYVAGGSSGGVAAAVAG
                     KLAAYGIGTDTGGSIRQPASFCGIYGLKPTYGSVSRYGIVAYASSFDQAGVLASCAED
                     TALVFDVISKYDERDMRSVGFKKSTYESLSGSVKGLKIGIATEDLMASDEFVNSAVEN
                     TINVFKSMGAEVEEIKIPFIKECLPVYYILACAEASSNLGRFDGIRYGYHVEDYEDLN
                     DMISRTRSEGFGDEVKRRIIFGTYVLSSGYFDAYYKKAQRLRESIIKEIDKIFKSCDI
                     VLAPTVPVAPLKAGANLSPVDLYRTDICTCAANIVGLPSISVPAGFTDDNMPVGIQLI
                     GGRFKEDVILNAAYAFEKETGGEYIKLSEMGCGV"
     gene            5799..7214
                     /gene="gatB"
                     /locus_tag="PLD98_03780"
     CDS             5799..7214
                     /gene="gatB"
                     /locus_tag="PLD98_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQA05402.1"
                     /translation="MMWEMVIGLETHVELSTKTKIFCSCTTQFGGEPNTHCCPVCTGQ
                     PGSLPLLNKEVVRYGVKAGLALGCHINLVSKMDRKHYVYPDMPKAYQISQFDIPLCEH
                     GKVELENGTTIRVNRIHIEEDAGKLIHEGSTYLVDYNRCGVPLIEIVTAPDFRSVEDV
                     LLYLEKLQGIMRTIGVSDCRMQEGSLRCDVNMSVRPKGSDKFGTRTEIKNLNSFTAIE
                     NAINYEFERQVDILESGGQVEQETLHFNSDTGETVSMRDKENANDYRYFREPDVVTVR
                     VSKEEYDELLASLPELPDQRMRRYVENLGLTEADAKLLVKYRRVAEFFEAAADIDAKT
                     AAAFILTNIFKSVPTEVEREEWQIKVSPENFRELVKLTSSGKINRNIAKRVLDRMLET
                     GSAPAEIIDKEKLGGVDEAEFEAICRKAIEENPAAVADYKGGKEKAFQALIGYVMRLT
                     RGAANPAQTEAKLKELLNEEA"
     gene            7222..7629
                     /locus_tag="PLD98_03785"
     CDS             7222..7629
                     /locus_tag="PLD98_03785"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HQA05403.1"
                     /translation="MKRASKHLIWGIVLIIIIAALIPAYFKFLKPPVNTDNSAVKNIV
                     VSVSYKDGTKKDFEISTTKSNLADALLEKGLVTSEDMADGFISSVDGITAETAKEEWW
                     KITKNGEDLMTGAKDTPINDGDRFELIFSVGFY"
     gene            7783..8943
                     /locus_tag="PLD98_03790"
     CDS             7783..8943
                     /locus_tag="PLD98_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQA05404.1"
                     /translation="MIKLIVKRFLKVKQNPNLENRHIYGLTASCVGIGLNVLLFVIKL
                     TAALLSGSVSIIADAVNNISDAASSVITLVGFKLSKQGADTEHPNGHGRVEYVASFLV
                     SLLIIVMGYESGKYAIKKIVSPEEITLDIITVVILLFSVLIKLYMYFYNKNIGKAINS
                     SSMIATAKDSISDVAATLGVLAAVLITRLTNFYADGFFGAAVSIFIVYTGLSSAKESG
                     TLLIGKAPSAEFIKRLENIVLSHKGISGIHDISYHDYGPERKMVSLHAEMSSEYDIST
                     AHDLVDIIERELFESLGVEAVVHIDPVCFDSHFEEIRDSLIKAIKEVNGNYSIHDLRV
                     LRTPEGLTLYFDTSVPYGDKTPDKEIKAQLTKAVKQLDPTYHSVITIDRRDV"
     gene            8959..10230
                     /locus_tag="PLD98_03795"
     CDS             8959..10230
                     /locus_tag="PLD98_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA05405.1"
                     /translation="MNSYKAEIIVVGGGFTGVAAAAAAAREGRDVWLIEKSGFLGGAA
                     TNCLVNPFMNYMFKDPKSGEAKPVNSGIFKEILGDLEAMGGLHKNRKTFNEEYLKIVL
                     DRLCKRYGVKVLLHTMVTDVYKEGDKITGIEVYSTGQKMSFFADCFIDCSGDGNLASL
                     AGCDFILGRETDNLCQPMTLCLRIANIKADKETVSKLRPKMNELFNKLQAEGKIKNPR
                     ENILFFEHMSEGVIHFNTTRIIKKNPISPFDLSQAEAEAREQGLELLNFLKENIPGFE
                     DATLIMTAAEIGVRESRKIIGEYILRAEDILSCKQFDDSIARGAYEIDIHNPGGTGTV
                     IKRIPEDKYYTIPYRSLVAKNLANLLVAGRCISSTHEAQSAYRVIPIVCCIGEGAGTA
                     AAVYIKNKNERMKDVDIKEVQSILDRNGALY"
     gene            10247..11221
                     /locus_tag="PLD98_03800"
     CDS             10247..11221
                     /locus_tag="PLD98_03800"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HQA05406.1"
                     /translation="MNYLVTGGAGFIGSHFTDFLLSEIGRDDMILCLDNLTYAGNLKN
                     LAEAERHENFRFVKGDICDKDFTDNVFSEFKPDVVINFAAQTHVDRSITSPEIFFSTN
                     LMGTVNLLELSVKHKVSLFHQVSTDEVYGDYSERESLYPNGFTEDASLLPSSPYAASK
                     ASAEMAALSFMRTYSLNISISRPCNNFGERQYPEKLIPLTVKNALEGKPIPLYGDGGS
                     VFREWVYVKDTCRAIYGIIQKRKNGCIYNISPSFSIDNKSLVEKILKIMNKPESLIAK
                     ADDRKGHDIKYRINSNKLLSELGFEFIYDFDESLERTVKSLIENESRG"
     gene            complement(11236..12048)
                     /locus_tag="PLD98_03805"
     CDS             complement(11236..12048)
                     /locus_tag="PLD98_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQA05407.1"
                     /translation="MINNKKIVLTGCNSGIGLEVLKILLKGENKILCVDKDIDVLKSL
                     NSEKIIILQKDVSSKEAVDEVFEFAEENLGIIDIFFANAGYAYYEEIDYTDWDRVERI
                     FKTNVFSPIYSYQKYIKHLGGRKGVFAVTISAIGTMAMPGFTLYSSTKFALHGFQQGI
                     RFEKPENMQLTCIYPVATNTNFFKTANQMDFKRPFPVQQPDVVARKAVKGIEKGRKTV
                     SPSALFIISQQLMKFLPFVRSAYWALENKKFREFRKRARENKAQTPKEEITV"
     gene            12180..>12672
                     /locus_tag="PLD98_03810"
     CDS             12180..>12672
                     /locus_tag="PLD98_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HQA05408.1"
                     /translation="MQNLIQRITAALALVFAVVSSFLGLPSVFEKYENSDKYDPTLGE
                     IKIDLDMTVPRLFLIENGESEYVIVKGSDAIESEAYAAEVLQDYLKRISGVQIPIVND
                     TAPQQEKEIIVGKTNREGENTFTVDREMLGDEGLRIFTYEDKLVIAGGEKRGALYGVF
                     TFLE"
     assembly_gap    12673..12682
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12683..14096
                     /locus_tag="PLD98_03815"
     CDS             <12683..14096
                     /locus_tag="PLD98_03815"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HQA05409.1"
                     /translation="ALGCRWFAADLTVVPELDIVSVRKDIDIEQIPYFYNRDVYWSCA
                     RDADFSVANKINGIVSLDLSGKYGGGGIKYGGPHFVHTFNFLVPPSVYFDEHPEYYAL
                     TANGIRETTQLCLTNPDVLRIATETVLSWLRADPTANVISVSQNDGKDGYCECPNCKA
                     IDDAEGSHSGTLLRFVNAIAEEVEKEFPNVYIDTLAYTYTRKAPKITKPRHNVVIRLC
                     TIECCFSHPLDECPVPDKSGKRITNDIREWAAICDNIHIWDYTTNFSHYLTPYPNFAV
                     LQPNVQFFAEHNVKSVFEQGCYQMVNNGEFAQLKAYLLAKLLWDPYTDIEKHMNEFML
                     AYYGEGYQYIRRYIDFAVDRAQLFHIHYNSSPEEIVYFSESDLKKIDEWFDKAESMAK
                     TQEELDRIQCSRIQIRYYKNFLRKGEFNIFNNTAATGEALYDDLMRFGITRTKERVPL
                     YPKEKINFRQKINTWYEQKK"
BASE COUNT         3931 a         3299 c         3446 g         3622 t
ORIGIN      
        1 gttgaatcgg taatggctgt aatcccacca tctggcgtgg aacagctttt ccataattac
       61 gctggtgatg aactccacta tgtcggcaag gataagacct attataaaca caagatacca
      121 tttatcttta aacggcgtaa ggcataccga taaaacaacc gctcccgtgc cgtatatggg
      181 gcaaatggga cctgttaaaa aacctctgtt tatccacttc ttttcgccga cggagcagta
      241 tatggactca accagccagc cgagggcact gtagaaaaag aaatataaca catagtcgat
      301 tactgttttc ataactctct taccttattg agtgtattaa gatttttttg aaacttttct
      361 ctatgacttt tgatgcggca gataagaatc tcctgccaag cgcagtattt gtccgcaagg
      421 gtgacgatgt aggactcttt gtatctggga ggggtgggag tcagaggcca catatggcgc
      481 tttatgatat cctcctcgat tttgttaaga acggtcagag ccctggcgtt tttcaaagca
      541 aagccggggt ggcggtagcc gtgcagtctg tggccttttt cgcccacatg ccagtcgtaa
      601 tagaaaaggt cgtggagtat ggctcccctg catacttctt taactctcgc gttatacttt
      661 ttggcaagca catagctcaa gtaagccacg ctgaggatat gctgcaccct gttaatgtcc
      721 ccgtgctgcg gatagacgga cattccctgc agctcttccc tgaaatacaa atcatctata
      781 tactgaaaga aatccgcgtc atcctctgtg ttcaaagaat atgagcggat ataggcgtca
      841 taaagaaaac ccacgcttta cactccgttt tagatattta ttatgaaaat tttatctgta
      901 ttcaaaaatt ttttcaagat gcttcgcgac ggcgtcttcg tcatggctgc ctattacgcc
      961 ggtggcgatt tttttaagct ggggcaccgc ttcgcttacc gcaaaagact cgtcggcgca
     1021 ctcaaacatt gagatgtcgt taagattgtc gccgaaaacg accgcccttt cggcgcccgt
     1081 catttctttt attctcagca tgccgctgca cttgctcgcc tttgagctga aaacctccaa
     1141 aaaccatgag tcctcaagat aagtgtcctc ataaaacgcc actgatacgc ccttgacgct
     1201 tctcagctta tccgcaaggg gctttaagcg gctgtagctg tcaacgatat taaagtaaac
     1261 gcactctccg ttgtcgggcg gagtgagctt ctccacctgc tcaaagcgtc tgtaatactg
     1321 cttttcccgc cttttataaa acatttcgtt ctgctttgag tcaattttct caaagaccac
     1381 atcgagcata tccccttccg cgcagtacat aaaggggctg acattaaagc ttttaaagat
     1441 ttcaatgatt ttaagcgctg tattcttttc aatgggaacg ctctcgatta ccctgccgct
     1501 tttaatgtcg gctataaaga cgccgttcat caaaacaacg gggaggttca aatcaaggca
     1561 ggaaagaatc cgctttgatg aggatacgct cctcgcggtg gcgacggtaa agagcattcc
     1621 tttagaaatc aggcggttta taatctcagc ggattttgcg cttatttcag cgtcggaagt
     1681 taaaagcgtt ccgtcaaggt cggaaatgta tacggttttc ttcataaact ctccaatgcg
     1741 tcacaaaata ttttcattag cttacgctta ttatttaatc aatttttgaa agaatacatt
     1801 tggtttagcg tcacaatgcc acttagagag tttagcatac ggcagtttcc tttgcaataa
     1861 atactaatct ccatatattg cttattggga aacaaaaaat taaggtttga ttaagacttt
     1921 gtttaaaaaa gtaaagtcat aactgcttga caagattttt agtctgtgtt aaatatataa
     1981 atgttatgtt agattaaact gctgacattt cggcacggca aaagactccg cttttgtgga
     2041 aagtctgaca gggagtccgc atcgtggact ggaagtgcgg atcagatgag tagtaagtcg
     2101 gggaacgctt ttaccgtatt ttttggggct ttgcccttac aagtaaacac gagtgagctt
     2161 tctttaaagc taatgcgggt ggcaccgcgg ggttagtata tcccgtcccg aaattgtcct
     2221 tataaggcag tttcgggctt tttttatagt atcaaaataa tagtactttc tttggagggt
     2281 aaaaaatgtc taaatataga actcataatt gcggagagct cagaacggag catataggtc
     2341 agaatgtcaa gctcgcgggc tgggtggaca ccgtcagaga ccacggcgga gtcattttcc
     2401 ttgatttgcg cgaccactac ggagtaacgc agaccgtttt ccatgacgag actatgcttg
     2461 agggcatttc aagggaatcc gtaataagcg ttgaaggcaa ggttgtaaaa agagatgagg
     2521 aaacagtaaa caacaaaatc gcaaccggtc aggttgaggt tgttgttgac aaaatagagg
     2581 ttctgggccc cgccattgcc cgcctgccct ttatccccgg tgagtccacc gccacaaggg
     2641 aggatgtaag gctcaaatac agattccttg acctgagaaa ccccgagatg cacgatgtta
     2701 tagttctgcg ctcaaaggtt atcagcttcc tcagaaagaa gatggaggag ctgggctttg
     2761 tggaaattca gaccccgatt ctcacctgct catcccccga gggcgcaagg gactatcttg
     2821 ttcccagcag aaagcacaaa ggcaagttct atgctctgcc ccaggctcct cagcagttta
     2881 agcagctttt gatggcatcg ggatttgata aatacttcca gatcgctccc tgcttcagag
     2941 atgaggacgc aagggctgac cgctcccccg gcgagttcta tcagcttgac tttgagatgg
     3001 cgtttgcaga gcaggaggat gtactgaagg tcgccgagac ggtgctttat gagacattta
     3061 ccgccttctc cgatttcccc gtatcaaagc ctccctttaa gagaatcccc tatgccgagg
     3121 ctatgctgac atacggcaca gacaagcctg acctcagaaa tccgcttatt ataaaggatt
     3181 tgacagacct ctttgccgat gttgacttcc ccgcattcaa gggcagaccc gtaagaggca
     3241 ttaaggcaga ctgcacgggc aaatcccgct ccttctttga gaatatgctc aaattcgccc
     3301 tttccatcgg catgaagggt ctgggttacc ttacccttgc gggcgacaca tttaaaggcc
     3361 ccatcgacaa gttcctgact cctgaaaaga aggcggagct tatcagaata gtcgacctta
     3421 aagacggaga gacagtattc tttatctgcg actctctgcc cattgtaaac acatacgccg
     3481 gtcaaatcag aacagagctt tgcaatcagc ttggtcttct cgataaagac cgctttgaga
     3541 tgtgctttat caccgacttc ccgatgtatg agataaatga ggagacgggc aagattgact
     3601 ttacccataa ccccttctcc atgccccagg gcggtcttga ggctctgaat aatcaggatc
     3661 cgcttacaat actcgcttat cagtacgaca cagtatgcaa cggcgttgag ctttcctctg
     3721 gagccgtcag aaaccaccat cccgaaatca tgcagaaggc atttgagatt gccggataca
     3781 gccatgaaga gcttaaagcc aaattcggcg ccctctatac cgcctttacc tacggcgctc
     3841 cgccccatgc gggcatggct cccggcgttg acagaatgat aatgctcctt gcgggcaaga
     3901 acaatatcag agatgtcatc gccttcccgc ttaactccaa cgctcaggat ttacttctgg
     3961 gcgctcccaa tgaggttacc gaaaagcagc tccgtgaggt tcatataaaa ctcagataac
     4021 cttgcgcaaa atataaagga tagggggatt ttatggtttc cgtcgataag attaaacgaa
     4081 tagcgaactt agccaaaatc cacatcagcg aagaagaagc aaaccagctc gcagagcaga
     4141 tgagcagcat tattgctttt gccgatgaaa taaacaactc ctccctgccg gtcagcgagg
     4201 aagagcttat caacgaatac gacagaacag agtacagaga ggacgaggtt cgcccctcat
     4261 ccgatattaa cgacatctta ctgaattcag gcggcactca gggcaacttc tttatggtaa
     4321 aagacacagc aaaggcaggc agaagatatg gttaatattt catattacaa aaagctcctt
     4381 gataccaagg ctctttcctg cacagaactg acaaaggcgt ttttggactc ggcagagaag
     4441 gaaaacccgt ccttaaacgc atatataagc attaccaaag aagaagcctt aaagacagcc
     4501 gctgaagttg acgagaagat agcaaggggc gaaagccttc agccccttga gggaattccc
     4561 gtaataatta aggacaatat ctgcacaaag ggcattagaa ccacatgcgc ctcaaaaatg
     4621 ctcgagaact atgtgccctt ctacgacgca accgtttggg agcatattaa ggctcagaac
     4681 tcggtgctca taggcaaggg caacatggac gaattcgcca tgggctccac cagcgaaaca
     4741 tcatatttcg gcattgtaaa gaacccccac aatcccgaat atgtcgccgg cggcagctcg
     4801 ggcggcgttg cggccgctgt cgcgggaaag ctcgcggcgt acggaatcgg cacagatacc
     4861 ggcggctcca taagacagcc cgcaagcttc tgcggaatat acggacttaa gcccacatac
     4921 ggctccgtat cccgctacgg catcgtcgcc tatgcctcgt cattcgacca ggcgggtgtg
     4981 cttgcaagct gcgccgagga cacagccctt gtatttgatg tgatttcaaa gtatgacgaa
     5041 agggatatga ggtccgtcgg ctttaaaaag agcacatacg agagcctcag cggcagcgtt
     5101 aagggtctta aaatcggcat cgcaaccgag gaccttatgg caagcgacga gttcgtaaac
     5161 tccgccgtgg agaacacgat taatgtcttt aagtccatgg gcgctgaggt tgaggaaatc
     5221 aagataccct tcatcaaaga gtgtctgccc gtttactata tcctcgcctg cgcggaggcg
     5281 tcctccaatc ttggaagatt tgacggcata agatacggct atcatgttga ggactatgag
     5341 gaccttaatg acatgatttc acgcacaaga agcgagggct tcggcgacga ggtaaagaga
     5401 aggataattt tcggcaccta tgttttaagc tcgggatact ttgacgctta ctataaaaag
     5461 gctcagcgtc tgcgcgagag cattattaag gaaattgaca aaatctttaa atcctgcgat
     5521 atcgttctgg ctcccactgt tcccgtggct cccttaaagg caggggcaaa tcttagtccc
     5581 gttgaccttt acagaacgga tatctgcacc tgcgccgcga atatcgtcgg tctgccgtca
     5641 atatccgttc ccgccggctt taccgacgac aatatgcccg ttggcattca gcttatcggc
     5701 ggcaggttca aagaggatgt tatactcaat gccgcttacg cctttgagaa agaaaccggc
     5761 ggcgaatata ttaaactttc ggaaatgggg tgcggcgtat gatgtgggaa atggtaatag
     5821 gtcttgagac ccatgttgag ctttccacaa agaccaagat attctgctcc tgcactacac
     5881 agttcggcgg cgagccgaac actcactgct gtcccgtttg cacaggtcag cccggctctc
     5941 tgcctttact caacaaagag gttgtgcgct acggcgtaaa ggcgggtctg gctctgggct
     6001 gccatataaa ccttgtttca aagatggaca gaaagcacta tgtttacccc gacatgccga
     6061 aggcttatca gatttctcag tttgatattc ccctgtgcga gcatggaaag gtggagcttg
     6121 aaaacggcac cacaatcagg gtaaaccgca ttcacattga ggaggacgcc ggaaagctca
     6181 tacatgaagg cagcacctat cttgttgatt acaaccgctg cggcgttccc cttatagaaa
     6241 tagtcaccgc acccgacttc agaagcgttg aggatgtgct tttgtacctt gaaaagctgc
     6301 agggcataat gagaactata ggagtttccg actgcagaat gcaggaaggg tcgctgcgct
     6361 gcgatgtcaa tatgtcggta cgcccgaagg gctctgacaa attcggtaca aggacggaga
     6421 taaagaatct caactccttt acagctattg aaaacgctat aaactacgag ttcgagcgtc
     6481 aggtggacat tctcgaaagc ggcggtcagg ttgagcagga aactctccac tttaactccg
     6541 acacaggcga gaccgtcagc atgagagaca aggaaaacgc caacgactac aggtatttca
     6601 gagaacccga tgttgtgacg gtcagggtat caaaggagga gtacgatgag cttttggctt
     6661 ctctccctga gctgcccgac cagagaatga gaagatatgt tgagaacctt ggtcttacag
     6721 aggctgacgc taagctcctt gtaaagtaca ggcgcgtcgc ggaattcttt gaggcggctg
     6781 cggatatcga cgcgaaaacc gccgcggcgt tcatccttac gaatatattc aaatccgttc
     6841 ccaccgaggt tgagcgcgag gagtggcaga ttaaagtttc gcccgagaac ttcagagagc
     6901 tggtaaagct cacaagcagc ggcaagatta acagaaacat cgcaaagcgt gttcttgaca
     6961 gaatgcttga gacgggctca gcgcctgccg aaatcatcga taaggaaaag ttgggcggag
     7021 ttgatgaggc tgagtttgag gctatttgca gaaaggctat tgaggaaaac cccgccgctg
     7081 tcgccgacta caagggcggc aaggaaaagg cgttccaggc tcttatcggc tatgtaatgc
     7141 gtcttacaag gggcgccgca aatcccgctc agaccgaggc aaagctcaag gagctgctta
     7201 atgaggaggc ttaacggtaa aatgaaaagg gcatcaaagc acttaatatg gggtattgta
     7261 ctgattatca ttattgccgc tcttatcccc gcctacttta agttcttaaa gccgcccgta
     7321 aatacggaca attccgccgt aaaaaatatt gtggttagtg tcagctacaa agacggcact
     7381 aaaaaggact ttgaaatctc tacaaccaaa agcaaccttg ccgacgctct tttggaaaag
     7441 gggcttgtca caagcgagga tatggcggac ggctttataa gcagtgttga cggcattact
     7501 gccgagaccg ctaaagagga atggtggaaa ataacaaaga acggcgagga tttgatgaca
     7561 ggcgctaaag atacccccat aaacgacggc gacaggtttg agcttatatt ttcggtagga
     7621 ttctattaaa aatagggcag agaatactct gccctatttt tttatgccga ttgagttttg
     7681 gggcgaattg tgatactctc taaataataa agtcatattt ttgtgaactt tcggtcgaaa
     7741 gtgaaataat atgactgtga gcattttttt aggagcagcg acttgattaa gttaattgta
     7801 aagcgatttt taaaggtaaa acagaatcca aacctcgaaa acaggcatat ttacggcctt
     7861 accgcttcat gtgtcggcat aggtcttaat gtgctcttgt ttgttataaa acttactgcg
     7921 gctctgctta gcggctcggt ttcaatcatc gctgacgccg ttaacaatat ttccgacgcg
     7981 gcttcctccg tcattaccct tgtgggcttt aagctatcaa agcagggggc ggataccgag
     8041 cacccgaacg gtcacggcag ggtagaatat gtggcgagct tcttggtgtc gctgcttatc
     8101 atagtgatgg gctacgagag cggaaaatac gccataaaga agattgtctc tcccgaggaa
     8161 atcactttgg atattatcac cgtcgttatt ctccttttct ccgttttgat taagctctat
     8221 atgtacttct acaataaaaa cataggcaag gcgataaatt cctcatccat gattgccacc
     8281 gctaaggaca gcattagcga tgtggcggct actctgggag ttttggcggc cgtactgatt
     8341 acgaggctta caaactttta cgctgacgga tttttcggcg cggcggtttc gatatttatt
     8401 gtttacacgg gtcttagctc cgccaaagag tcgggtacgc tgcttatcgg caaggctccg
     8461 tctgctgagt ttataaaacg gctcgaaaat attgtcctct cccacaaggg catatcgggc
     8521 attcacgata tttcgtatca cgactacggc cccgaaagga aaatggtatc ccttcacgcg
     8581 gagatgtcat cggaatacga catcagcacc gcccacgacc ttgtggacat tatcgaaagg
     8641 gagctttttg aaagccttgg ggttgaggcg gtagttcaca tagaccccgt ctgtttcgac
     8701 tcccattttg aggagataag agacagcctt attaaagcta taaaagaggt aaacgggaat
     8761 tactccattc acgatttgag ggttctgagg accccggagg gtcttacctt gtattttgac
     8821 acaagcgtgc cctacgggga caagacacct gataaagaga ttaaagcgca gcttaccaag
     8881 gctgtaaagc agctcgaccc cacatatcat tcggttataa ccatagacag aagagatgtc
     8941 tgatagagga gatatattat gaattcgtat aaggctgaaa ttattgttgt cggcggaggc
     9001 tttacgggag tcgcggctgc cgcggcggcc gcgagagaag gcagagatgt ttggctcata
     9061 gaaaaaagcg gctttcttgg cggagccgcc accaactgcc ttgtaaaccc ctttatgaac
     9121 tatatgttca aagaccctaa gtcaggcgaa gcaaagcctg taaacagcgg catttttaag
     9181 gaaatactgg gtgaccttga ggctatgggc ggactgcaca agaaccgcaa gactttcaat
     9241 gaagaatacc ttaaaatagt tctggacagg ctgtgcaaaa ggtacggcgt caaggtgctt
     9301 ttacatacga tggtcaccga tgtgtataaa gagggcgaca agataacggg cattgaggtt
     9361 tacagcacgg gtcagaaaat gagtttcttc gcggactgct ttattgactg cagcggcgac
     9421 ggaaatctcg cttctcttgc gggctgtgac tttatcctcg gcagagaaac ggacaatctc
     9481 tgccagccca tgaccttgtg ccttagaata gcgaacatta aggcggataa ggaaaccgta
     9541 tccaagctca gaccaaaaat gaacgagctt tttaacaagc tgcaggcaga gggaaaaatc
     9601 aaaaacccca gagagaacat tcttttcttt gagcatatgt ccgagggagt aattcacttt
     9661 aacacgacaa ggataataaa aaagaacccc atctcccctt ttgatttaag ccaagccgag
     9721 gctgaggcaa gggagcaggg tttggagctg cttaatttcc ttaaagagaa catacccggc
     9781 tttgaggacg ctactttaat tatgaccgcc gcggaaatcg gagtcaggga aagcagaaag
     9841 attatcggtg aatacatact cagggctgag gacattttaa gctgcaagca gttcgatgac
     9901 tctatcgcac ggggcgcgta cgaaattgac attcacaacc ccggaggcac cggcacggtt
     9961 atcaagcgca tacccgagga taaatattac acaattccat acaggtcgct tgtggccaaa
    10021 aatctcgcca atctccttgt tgcgggtagg tgcatttcct ccacccatga ggcgcagtcg
    10081 gcatacaggg ttattccgat tgtctgctgc ataggcgagg gcgcgggtac cgcggcagcc
    10141 gtttatataa agaacaaaaa cgagagaatg aaggatgttg acattaaaga ggtacagtca
    10201 atactcgaca gaaacggcgc actttattaa agagatgtga gggtgtatga actatcttgt
    10261 aacaggcgga gcgggcttta taggcagtca ttttacagac ttcctgctga gcgaaatcgg
    10321 cagggacgat atgatattat gccttgacaa ccttacatac gcgggaaatc ttaaaaacct
    10381 tgccgaggct gagaggcacg aaaatttccg ctttgttaag ggagatattt gcgacaagga
    10441 ttttaccgat aatgtttttt ctgagtttaa gcccgatgtc gtgattaact ttgccgctca
    10501 gacccatgtt gaccgctcca ttacctcccc cgaaatcttt ttcagcacta atcttatggg
    10561 cacggtaaat ctccttgagc tgtcggtaaa gcacaaagta tctctctttc atcaggtttc
    10621 aaccgatgag gtttacgggg actattcgga gagggagagc ctctatccaa acggctttac
    10681 ggaggatgct tcgcttctgc cctcaagccc ctatgccgcg tcaaaggcgt ctgcggagat
    10741 ggcggcactc tcctttatgc gtacatactc tcttaacatc agcatctccc gcccctgcaa
    10801 caacttcggc gaaaggcagt atcccgaaaa gctgattccc ctgacggtca aaaacgccct
    10861 tgagggaaag ccaattcccc tttacggcga cgggggcagc gttttcaggg agtgggttta
    10921 tgtaaaggac acttgcaggg cgatttacgg cattatacaa aagcgcaaaa acggctgtat
    10981 ttacaacatt tcaccctcat tcagcattga caacaaaagc cttgttgaga agattttgaa
    11041 gataatgaat aagcccgaaa gccttatcgc aaaggcggat gacagaaagg gtcacgacat
    11101 taagtacagg ataaattcaa ataagcttct ttccgagctg ggctttgagt ttatatatga
    11161 ctttgacgaa agccttgaga gaacggtaaa aagccttata gaaaacgaga gccgaggtta
    11221 aacctcggct ttttgttata cagttatttc ttcttttggg gtttgggctt tgttctccct
    11281 tgcccttttt ctgaactccc ggaacttctt attctccaaa gcccagtatg cgcttctcac
    11341 aaagggaagg aatttcataa gctgctgtga gattatgaaa agtgcgctgg ggctgactgt
    11401 ctttctgccc ttttcaatgc cttttacggc ctttcttgcc accacatcgg gctgctgtac
    11461 ggggaagggc cgtttaaagt ccatttggtt agcggtttta aagaagtttg tgtttgtggc
    11521 gacgggataa atgcaggtaa gctgcatatt ttcaggcttt tcgaacctga ttccctgctg
    11581 aaaaccgtgg agggcgaatt ttgtcgatga ataaagggta aagccgggca ttgccatggt
    11641 gccgatggcg gagattgtga cggcgaaaac tccctttctg ccgccaaggt gctttatgta
    11701 tttctggtat gaataaatcg gggaaaagac attggtttta aagattctct ccaccctgtc
    11761 ccagtcggtg tagtcaatct cctcataata ggcgtagccc gcgttggcaa agaaaatatc
    11821 gataatgcca aggttttctt ccgcaaactc aaaaacctcg tcaaccgcct cttttgagga
    11881 tacatccttt tgaaggatga tgattttctc gctgttaagg cttttcagca catctatatc
    11941 tttgtcaacg cagaggattt tgttttcgcc tttgagcagg attttcaaaa cttcaagacc
    12001 tatgccgctg ttgcagcctg tgaggactat ttttttattg tttatcatac tgcccctcct
    12061 ttattgccgc tttgcataaa aattattctc cttgcctgca ttttatggta aattttgctt
    12121 gaatacaaga gtaaagttta gtattatatc tttatcaatc agaaaatgga ggaatgggca
    12181 tgcagaacct tatacagcga attacggcgg ctttggcgct ggtttttgcc gtagtttcga
    12241 gctttttggg attgccttct gtttttgaaa agtacgaaaa ctccgacaaa tacgacccga
    12301 ccttgggtga gattaagatt gaccttgaca tgacggttcc gagacttttc cttattgaga
    12361 acggggaaag cgagtatgta atcgtcaaag gcagcgacgc gatagagagc gaagcatatg
    12421 ccgccgaggt tcttcaggat tatttaaaga gaatcagcgg cgttcaaatc cccattgtaa
    12481 acgacaccgc cccccagcag gaaaaggaaa ttatcgtggg caagacaaac cgcgagggtg
    12541 agaatacctt taccgttgac cgtgaaatgc tgggcgacga aggtctgagg atttttacat
    12601 atgaggataa gctcgtaatc gccggcggag aaaagagggg cgcgctttac ggcgtgttta
    12661 ccttccttga ggnnnnnnnn nnggctctgg gctgccgctg gttcgccgca gaccttacag
    12721 tcgtgcccga gcttgacata gtcagcgtcc gcaaagatat agatattgag cagattccgt
    12781 acttctacaa ccgcgatgta tactggagct gcgcaagaga cgcggatttc agcgtggcga
    12841 acaagataaa cggcatagta tcccttgacc tttcgggcaa atacggcgga ggcggaatca
    12901 aatacggcgg tccgcacttt gtccacacat tcaattttct cgtgccgcct tcagtttact
    12961 ttgatgagca ccccgaatac tatgctctta cagcaaacgg cataagagag acaactcagc
    13021 tttgccttac aaatcccgat gttttaagga ttgccacaga aacggttctg agctggctga
    13081 gagccgaccc caccgctaat gtgatttccg tatctcagaa tgacggcaag gacggctact
    13141 gcgagtgccc gaactgcaaa gccattgacg acgcagaggg cagccattcg ggaactctgc
    13201 tgcgctttgt aaacgctatt gcagaggaag tggaaaagga attcccgaat gtatatatcg
    13261 acacccttgc ttatacctac acccgcaagg ctccgaaaat cacaaagccg aggcacaatg
    13321 tggtcataag gctttgcacc attgagtgct gcttctccca tccccttgac gagtgccccg
    13381 ttcccgacaa atccggcaag agaataacca acgacataag agaatgggca gctatatgcg
    13441 ataacattca tatttgggat tacaccacaa acttctccca ctaccttact ccttacccga
    13501 actttgcggt tctgcagccc aatgtccagt tctttgcgga gcataatgta aagagcgtct
    13561 ttgagcaggg ctgctatcag atggtaaaca acggcgagtt cgctcagctc aaggcttatc
    13621 tgctggcaaa gctcctttgg gacccctata ccgatattga aaagcacatg aacgagttta
    13681 tgctggcata ctacggcgag ggctatcagt acatccgcag gtacattgac tttgccgttg
    13741 acagggctca gcttttccac atccattaca atagcagccc cgaagagatt gtttacttct
    13801 ccgaatccga cttaaagaag attgacgaat ggtttgacaa agcagagtca atggccaaga
    13861 ctcaagagga gcttgaccgc attcagtgct caaggataca gattagatat tataaaaact
    13921 tccttagaaa gggcgagttc aatatcttca acaacaccgc cgctaccggc gaggccttgt
    13981 atgacgacct aatgcgcttc ggcataaccc gtacaaagga aagggttccg ctgtatccca
    14041 aggagaaaat aaacttcagg caaaaaatta acacctggta tgagcagaaa aagtaaatga
    14101 taaaaatccc cgctttaccg cggggatttt ttgcgctttg ccgaaaaaga taaattaagc
    14161 aaattcatat ttttagtatg aaaactttga tttttggaag ttttatgcta aacttaatag
    14221 agatttatga gaataaggac aggatataaa tgaagtatgt cttttccgac agaatttccg
    14281 cgctgaagcc atcggctata agggaaat
//
LOCUS       DAONVQ010000038        14222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3252_length_14222_cov_4.840827, whole genome shotgun sequence.
ACCESSION   DAONVQ010000038 DAONVQ010000000
VERSION     DAONVQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14222
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3252_length_14222_cov_4.840827"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3315)
                     /locus_tag="PLD98_03820"
     CDS             complement(<1..3315)
                     /locus_tag="PLD98_03820"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HQA05410.1"
                     /translation="MLMSESRMTSYYAVSSRIVPKKHWGALGRTLAKAGRYTGLISWT
                     GTMFEYYMPHLFLPVYGGTLVSEALRFCLWCQKERTRKLGIPYGISESGFYAFDPQLN
                     YQYKAHGVQKLGLKRMLNADLVISPYSTFLTLPFDRSAAMKNLAALEKLQLVGRCGFY
                     EAADFTPERTQGQDYSVVRSYMAHHLGMSMLSILNAVEANIMQKRFMRDEKMAAGRCL
                     LEEKIPSGAVVFNDVEQRDIPARPERIVPTNKRIDEINPLFPECRLYSNGEWTSITAD
                     TGSSASLYRGADITRRGSDFRNPGGIFAFLKTNKGEVIPFTMSPSVSAKGSFKARLSH
                     YAAELMSQSEIAECTETISVHPRIPAERRRFTIKNTGKQAIRGELFIYFEPSLTSHIS
                     ESSHPAFSKLFLKSFYNRQEKVLLFERRGRQNEPLCLAAGFIEDRKFTYDTRREHVLE
                     RPMGPESLLRPNIRLENKTGSVDNCAAFSVPVNIQPKASESITLVLSAASTSQEALER
                     FLKIRSENLPITKGAPSPFNETGLDGVGASRLLTSVFFRRTMTTDAKTAARDLSGSVK
                     ALWSVGVSGDNPIITVTAERHEDLTNAIPFIRMHKKLSLCGIPTTLVILIKENEGYEA
                     RNIQTIRNILRKENHEQALGSGAGIIPVNINSAGEAALKALRAAAVLSYPESSALPKT
                     VKENIKMLPILPSEPPEDSAKENRFENGGFVVADKPVLPWSFVLSNERFGTLVSDSAL
                     GYTWAVNANLNKLTPWVNDTMRDNRGEMLLLKSDDKLIDLVMGSEAFFRTDFVRYRGR
                     AEGLKYEITVSVEKDKTAKIVEAELENPTDREKVFELAYYIKPVMGQDSSGAVFLKAD
                     KIDGGIKISSPIDAEFPGVIHLTADEAKGICSDDTDFFSGRWNKCGLQPSPDICGAVI
                     RQLILPPRGKEKVKFILSWHSLLEINEEDYDMPEYRLESKFKEPVVIGKGVTEHCEEE
                     REFDALEGEPNLYKELNEKGRELLTNEDKIAESEDYITESPHEEPTLAPEDTAFSDER
                     RLTEETSEGETFVKPRSKAAPPINGSEPSGLEREEIYDHEVLKGEPNLLEELNEKGRE
                     LLKDDKAAETQHFFTES"
     gene            complement(5309..6304)
                     /locus_tag="PLD98_03825"
     CDS             complement(5309..6304)
                     /locus_tag="PLD98_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05411.1"
                     /translation="MKYLSKKLKDDLSLLRNAYRKTLYYEGTDNSFEWLSDNYHILER
                     EGRNILKELRVGGAEKSMDFSRVYTLCREICTDGILPSGDTITDTLRDKELSVFECER
                     LPLVFRAVLLSYAADSWRKRLREIPKTPRRKEHVYTEPLIQVEDESINAIKSLRRIQD
                     IDFSELIEKISPVEKILSEDPAGIYKNMDEATRARYRRAVSKMAARESITEIAAAKRA
                     IEKARKSEGRERHVGFHLDIVKKGSGRRGFALILMEPFIALVLAVSLYVATGLWYLPF
                     ILFLPLWEIVKVGTDLAAREAQPLCAAQNGAGRGHPRKRPHSYNHLNAAALRGQG"
     gene            7197..7598
                     /locus_tag="PLD98_03830"
     CDS             7197..7598
                     /locus_tag="PLD98_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQA05412.1"
                     /translation="MKRHSKQRELIMKSLKKLDHPTAAEIHDDIKLEYPKISLGTVYR
                     NLSELADSKIILRLPSSDGADRFDWNTDEHFHAHCTVCGKVFDLFGLLPREAVDSLDK
                     AVEEHTGLSVSSREISFKGICKDCAKNKNFQ"
     gene            7648..8211
                     /gene="ahpC"
                     /locus_tag="PLD98_03835"
     CDS             7648..8211
                     /gene="ahpC"
                     /locus_tag="PLD98_03835"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HQA05413.1"
                     /translation="MSLVGKKVSDFKVKAFHNGEFIDVTSDDIKGKWSVFVFYPADFT
                     FICPTELADLADLYDKFKEIGCEVYSVSTDTHFVHKAWHDASDTIKKIKYPMLADPTG
                     KLSRDFDVYLEDQGLALRGSFIVNPNGEIVAYEVNADNIGRDASELLRKVQAAQFVWE
                     HGDEVCPAKWKPGEKTLKPSLNLVGKI"
     gene            8275..9903
                     /locus_tag="PLD98_03840"
     CDS             8275..9903
                     /locus_tag="PLD98_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA05414.1"
                     /translation="MDTKNLYDLIILGSGSAGMSAGIYAGRARLKTLVIDKARAGGQI
                     SITNEVANYPGVLNISGPELSDNMKKQAQSFGAKFLDAEVTAVDLEGDIKTVRTTNGD
                     YKALSVIAATGAVPRVLGFKGEQEFRGRGIGYCATCDGEFFTGMDVFVIGGGYAAAEE
                     AVFLTRYAKKVRLIVREPDFTCARAVAEKALNHPKIEVFFNTEIVEVGGDNVLKYAEF
                     VNNQTGERTRYEVPEGDLTFGVFIFAGYVPQSKLFEGVLELDEFGYVKTDEDMRTNIE
                     GVFAAGDIRPKKLRQLVTAVADGAIAAIEAEKYVNEKKRQLGIAETEESGEENADGEF
                     LDDEIKEQLKDIFERFERKVTIAAVLEGDEELSSELKGFLTDFAALSDKVELKFISGS
                     ELKALGASADVLPTAAVLDADGKYTGMQFGGVPGGHEFNSFVIALYNAAGPGQEISDE
                     TMSKISSINKKANLKIGISLSCPLCPDVVAASQLIALKNRNVEAQMVDISRFPEIKNK
                     YAIMSVPALIVNDEKVHFGKKSIEEIAAIIMAES"
     gene            10146..12116
                     /locus_tag="PLD98_03845"
     CDS             10146..12116
                     /locus_tag="PLD98_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05415.1"
                     /translation="MKKFLAVLLSFVLIFTSVITVFAEAGEEPEFYTLNLDKLYEEGA
                     LPEGVTFTYDAGIDLNRIPANTYVSLKFKVPKQYNTARLFLKVSPNPEFVDYDYTILR
                     SSADGTFLIYMNSDKSLAYDLFEIKSFYIDVIVMEEYAIRQCEPYDENDPTPFEVIPG
                     GRVVQYGGDYWFKLILAPDYSNSDVVVSVNGKVIQPAGYDADGNRVYHLKNITAQPVF
                     SVTGLLPNTANSIFEWILKIMRLIAGIIKGWFNRNPEPTTTEPTTEFTTDPEKVYYSA
                     SMPPSHEWAYTVTPLNNPYYVLEGSDFKFRVDKAEGAVGNIYVYVDGEIAEPDASGVY
                     TVREVSADFAVAVRAILFSLPAENAAYTFTPAIDPTEVQPGGSFSFTVTKSELVTGNI
                     VVKANGTVINPTSGGAYTISNINNPVKIEVQVVSVTLPASVPGIYAVIPLTSAIEIPI
                     GGSFSFRIEKNANYLGNFVIKANGTVINPVGGVYTVTNITGPVTITIGAHLVKLPQIE
                     PNPYYTVQYLTDPSTLGEGGVYRIKITRLGSFNGGISVLANGVSVDPSESDLTIYEIP
                     VNSPVEIDIQACTVLITGLDNPVIRMTAVSDLNNIPVGGSFSFQLELVTGYTGEIVVK
                     ANGREIAPNSAGVYTVSNIRGPVTISIAVYFS"
     gene            12182..12919
                     /locus_tag="PLD98_03850"
     CDS             12182..12919
                     /locus_tag="PLD98_03850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQA05416.1"
                     /translation="MKIPKLALFDFGGTLADDGELDIDSGLKAVLNAAGVKEYDEDLL
                     RNLWIDMQEQVLSDIRTPKGYRVETPLSAIFRNIFARAGIKVDLPLTECEYIFDKFNS
                     TRKRTPYIDELLNVMREKGIRTGVISNIVMSGESLKMAIDELLPDNKFEFILTSCDYL
                     FQKPAPHMFEAAAKIAGVEPQDCWYLGDSYRADVLGASPVGMTAFRYKKEQEEEFLTL
                     TAEIDGKEYTYYGINSWKVLANMLREN"
     gene            12935..13963
                     /locus_tag="PLD98_03855"
     CDS             12935..13963
                     /locus_tag="PLD98_03855"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HQA05417.1"
                     /translation="MEQVRITEPTLLLGEDGNVLKPGYCTTNLYVYNRENIKAHPSRI
                     KEWDFYQISNDRYTVQIVFGDISFAGFGSFAFFDRETGEREDALSVIPLTFGKMNMPR
                     TTETPHKISVGYAGTKLRVVAAKNKRYLTADIRARRNRIKADLELEVMDGLESLVMAV
                     PFEDLPGYFYLNQKMNSMPVKGYVEIGSRIFEFSPQNSFAVLDWGRGVWPYKCHWYWG
                     NGTTRLEDGSLFGFEIGWGFGDMSAATENMLFYNGKGHKIGEVHLENNNHKDYMQPWV
                     FSSSDGRFEMTMTPEYDNYTSSRIGPIGNRCHQVYGKWNGTVVLDDGKELHIKDMTAF
                     CEYSDNRW"
BASE COUNT         3461 a         3767 c         3703 g         3291 t
ORIGIN      
        1 cgattcggta aagaagtgct gagtttcggc agccttgtca tctttcaaaa gctccctgcc
       61 tttttcgttc agttcttcaa ggagattcgg ttcgcccttt aatacctcgt ggtcataaat
      121 ctcctcccgc tccaagcccg agggctctga gccgtttatc gggggagcgg ctttggaccg
      181 aggcttgacg aaagtttctc cttcggatgt ttcctctgtg agccttctct cgtcggagaa
      241 cgccgtatcc tcgggggcga gggtcggctc ctcgtggggg gattcggtga tataatcttc
      301 gctttctgct attttatctt cgttcgtcag cagctccctg cccttctcgt tcagctcctt
      361 ataaaggttc ggctcgccct caagggcgtc aaattctctc tcctcttcgc agtgttcggt
      421 gacgcccttg ccgataacga caggctcttt gaatttgctt tcaaggcggt attcgggcat
      481 atcgtagtct tcctcgttta tttcgagcaa agagtgccac gagagaataa actttacctt
      541 ttcctttccg cggggcggca ggataagctg ccttatgacc gcgccgcaga tgtcggggga
      601 gggctggaga ccgcacttgt tccatctgcc cgagaagaag tcggtgtcat cggagcagat
      661 gccctttgct tcgtcggccg taaggtgaat tacccctggg aattccgcgt cgatggggga
      721 gctgattttg atgccgccgt ctatcttgtc ggctttcagg aatactgcgc cggagctgtc
      781 ctgacccatg acgggcttaa tataatacgc cagctcaaag accttttccc tgtccgtcgg
      841 gttctccagc tcagcctcca ctatctttgc ggtcttgtct ttttccacag atacggtaat
      901 ttcgtacttc agcccctcgg ctctgcctct gtagcggacg aaatcggtcc tgaagaacgc
      961 ctcgctgccc attacaaggt caatcagttt atcatcgctc tttaaaagca gcatctcgcc
     1021 gcggttgtcc cgcattgtgt cgttgaccca cggggtgagc ttattgagat ttgcgttcac
     1081 tgcccatgtg tagccgagag ccgagtcgga aaccagagtt ccgaatcttt cgttgctgag
     1141 aacaaagctc cagggcagaa cgggcttgtc cgccaccaca aagccgccgt tttcgaagcg
     1201 gttttctttt gcagagtctt cgggaggctc ggaggggaga atcggcagca tctttatatt
     1261 ctcttttacg gtcttgggca gggcggagct ttcgggatag cttaataccg ccgccgctct
     1321 aagagccttc agcgccgcct cgccggctga gttgatgttg acggggataa tgccggcgcc
     1381 ggagcccaga gcctgctcgt gattttcttt ccttaatata ttccttatgg tctgaatgtt
     1441 ccttgcctca tagccctcgt tttccttaat cagaataaca agggttgtgg gtatgccgca
     1501 gagggagagc tttttgtgca ttcttataaa gggaatggcg ttggtaaggt cctcatgccg
     1561 ctcggctgtg acggtgatta tgggattgtc gcccgacacg cccacgctcc aaagagcctt
     1621 taccgagccg ctcaaatccc ttgcggctgt tttggcgtcc gtggtcatgg ttctgcggaa
     1681 gaatacggag gttaaaagcc tgcttgctcc cacgccgtca agacccgttt cgttaaaggg
     1741 cgagggcgcg cctttggtta tgggcaggtt ctcggagcgg attttgagga aacgctccag
     1801 agcctcctga cttgtggacg cggcgctgag aacaagggta atgctctcgc tcgccttggg
     1861 ctggatatta acggggaccg agaacgccgc gcagttgtca acactgcccg tcttattctc
     1921 gagccttata ttcggtctga gaagggactc cggtcccatg ggcctctcga gcacatgctc
     1981 gcgccttgtg tcgtaggtga attttctatc ctctataaat cccgccgcaa gacaaagcgg
     2041 ctcgttttgc cttccccgtc tttcaaaaag cagtaccttc tcctgacgat tgtagaaact
     2101 ctttaggaac agctttgaga aagcgggatg gctggactcg gaaatatggg aggtcagcga
     2161 cggctcaaag tagataaaca gctcgccgcg gattgcctgc ttgcccgtat ttttaattgt
     2221 gaacctgcgt ctttccgcgg ggattctggg gtgaacggaa attgtctcgg tgcactcggc
     2281 aatctccgac tgggacataa gctcagccgc gtagtgggag agccttgcct taaaggagcc
     2341 ttttgcgctg acagacgggc tcatggtaaa gggtatgacc tcgcccttgt tggtctttaa
     2401 gaaggcgaat attccgccgg ggtttctgaa atccgagccc cttctcgtta tatccgcgcc
     2461 ccgatagagc gatgcggagg aaccggtgtc ggcggtgatg ctggtccatt cgccgttgga
     2521 gtaaagacgg cactcgggga acagggggtt aatctcgtca atcctcttgt ttgtgggcac
     2581 gattctttcg ggccttgcgg gaatgtccct ttgctccaca tcgttaaaga ccacggcgcc
     2641 ggagggtatc ttttcttcaa gcaggcacct gcccgccgcc attttctcgt ccctcataaa
     2701 acgcttttgc atgatgttgg cttcaacggc gttgagaatg gacagcatgc tcatgccaag
     2761 atggtgcgcc atatagctgc gcacaacgga ataatcctga ccctgagttc tttcgggggt
     2821 aaagtctgcg gcctcataaa agccgcacct gcccacaagc tgcagttttt caagggcggc
     2881 aaggttcttc atcgccgcgc tcctgtcaaa gggcagagtc aggaaggtgg aataggggga
     2941 gattacaagg tcggcgttga gcattctttt aagacccagc ttttgaacgc cgtgggcctt
     3001 gtactggtaa ttgagctgcg ggtcaaaggc gtagaagccg ctttcggaaa ttccgtaggg
     3061 gatgcccagt tttctcgttc tttccttctg gcaccacaga cagaatctca gagcctcgga
     3121 aacaagggtg ccgccataga cggggaggaa gagatggggc atgtagtatt cgaacatggt
     3181 gccggtccag gagataagac ccgtatatct gcccgccttg gcaagagttc tgcccaaagc
     3241 tccccagtgc tttttgggca cgattcggct cgacacagcg tagtagcttg tcattctcga
     3301 ctcgctcatc agcatatcgt agtaggagtt ggacatctcc ccgctttcaa ggtcgtagcc
     3361 tatatgaaag aactttctct gacggttgta gaaaaccgac aaatccgtat tgtcaataat
     3421 tcttgttatc ctctccgcaa tctggtttac ctgctcatcc tcgtgggcgt actcgtaaag
     3481 tccctgcctt aaagccacaa ggcagcagat aaagttgccg ctgtccacgc tggaggcgta
     3541 tctgggatgg agaggcttta agctctgagt gtcgtaccag ttaaggagat tgccgttcca
     3601 cttctccagc ctgtctatgg tgtcaagggt gtttgaaagc ctttgacaca tctgctttgt
     3661 gtcaataaag ctgaaatccc ttgccgccag aatacagcag agcattaaac ctatattcgt
     3721 cggcgatgtc ctgtgggctg tgcggtgaac gggtgtctcc tgcacattgt cgggcggcag
     3781 gtagttgtcc gacgccctgc agaagctgtc gtagaatttc catatagacg ccgccatcgc
     3841 cttcattttc tccttttcct cgctgtcggg gagagacctt gaggccttct tctccctgcc
     3901 cgagaagtag gcgaagaaaa cattgtggac aaacagaagt cccgcaagcg ttgagaaggg
     3961 agtgccgaag attataagga gcatacccgc gataagcgaa ggcagacagc gttttaggac
     4021 ttttacaaag ccctttgatt tgtcaaagaa ggcggcgggc acccactcaa ggagcttttt
     4081 cttagagatt agctgcctgt aaacagcctt gcagatggcg tcaagggaaa cccacgccga
     4141 ctgcataaga ctgcaggcgg aaacgaaagc ccttataaac gacgccagag cgtcggggat
     4201 agcggaggaa taatatagcc ttgagagcat tgaaaagccg ccaccgatga tggaattaaa
     4261 gcctgcgaac agctcgccca cgccggcgct gataaggcag ataagggtgg taagacccgc
     4321 ggcgaggggc tgcatgaaga ttgcgagaag aattccgatt gccgcgaaaa cgggggtaac
     4381 ggaacgcctt aaattatcga aaaccttata tctcgaaacc acatcaaaga cattcttctg
     4441 ctttatgccg tcctttttta tcctcggcag gcagtatctg aggttctgcc agtcgcctct
     4501 tatccagcgg tgcagacggt catagaagga gagctgattt gcggggaagg agtcggtaag
     4561 ctccacatct cccataaggg cggtccttaa aaagatgccc tctaaaatat cgtggctcag
     4621 aattctttcg gacgggaaac ggtcctttaa aagagcgtgg aaaacatcca catcaataag
     4681 acccttgccc atgaaaatgc ttgagccgaa catatcctga tacctgtccg cggctacgga
     4741 gttgtaggcg gtaatgccgc cggtgcccgc cattatccgc gcgaatgagg tctttgccgc
     4801 tgaggaaatg tcggtcacaa ccttgggagc gaaaatgccg tagccctctg tgacgatgcc
     4861 ccttttcttg tcaacaacgg ggatgttgag aggatgaacc gccgcgctga ccagctccga
     4921 cagagtgtca aggggcatat tggtgtcgga gtcgagagcc attatatatt tggttttctg
     4981 cagattctcc gtatcgccgt aaaggcacaa gaattcgtcg ctctcgccct tgattgcgga
     5041 aacaagagcg gtgattgcgc ctctttttct ctccctgccc gtatattcgt tctgggtggg
     5101 ggagaaaatc ctgggtctga cggcaagtat aaagccgccg ccgtactttt gattgagctt
     5161 gtctatgacc cgctttgaag cctcaatgtc cgattcgtcg gtgggctttg tgggcgtttt
     5221 ggctccttta aggtcagcga gaacgcatat ggacacagcc cccgacttgt ttgtgcggta
     5281 aagctgggac agatgcttta tcaagtcctt agccttgtcc gcggagggca gcagcgttga
     5341 gatggttata agagtgcggg cgttttcggg gatgaccccg tccagctcca ttctgggcag
     5401 cacatagggg ctgtgcctct cttgcggcaa ggtcggttcc cacctttaca atctcccaaa
     5461 ggggaaggaa gagtataaag ggcaggtacc aaagccccgt cgcaacatag agggatacgg
     5521 ccagaacaag agcgataaag ggctccatga gtataagggc aaagccccgt ctgccgctgc
     5581 cctttttgac tatgtccaga tggaagccca catgtctttc tctgccttcg ctcttcctag
     5641 ccttttcaat agccctcttt gcggcggcga tttcggtgat tgactccctc gccgccattt
     5701 ttgaaaccgc cctgcggtaa cgggcccttg ttgcctcgtc catgtttttg tatatgcccg
     5761 cagggtcctc gcttaagatt ttttcaacgg ggctgatttt ctcaataagc tccgaaaagt
     5821 caatatcctg tatgcggcgc aaggacttaa tggcgttaat gctctcgtcc tcgacctgaa
     5881 tcagaggctc tgtgtaaaca tgctcttttc ttctcggagt cttgggaatc tcgcgaagtc
     5941 ttttgcgcca gctgtcagcg gcgtaagaga gcaaaacagc cctgaagaca aggggcagcc
     6001 tctcgcactc gaatacggac agctccttgt cccttaaggt gtcggtaatg gtgtcgcctg
     6061 aaggcagaat accgtcggtg cagatttccc tgcaaagggt gtagacgcgg gagaaatcca
     6121 tgctcttttc agccccgccc acgcggagct cttttaggat atttctgccc tcccgctcta
     6181 aaatatgata gttgtcggac agccactcaa agctgttgtc cgtaccctcg tagtagagcg
     6241 tttttctgta agcgttcctt aaaagtgaaa ggtcatcctt taattttttg gaaagatact
     6301 tcatgcgaat accatcctat aagatgtaat cctttctatt gtttacagaa atagctaaat
     6361 ttaaacattt ttatgtaaac gactttccgg ctgaattatt atttccggct cccgccttta
     6421 tataaaagga aaggtaacat aaaggaaaac acaccctata aaatctttgc ccgcctgagc
     6481 aaaaattaat caccaaaggc gcatatttgt tttcccgcca acgagtgaga gcatccacca
     6541 cgccgccgag gggagcggtc acgccgccga ggagtattaa ccgctgagta ccgcccgcca
     6601 ccgagatggt tgcgcccgac actgcggggg agttcccgcc gcccgcttgc caaaagagaa
     6661 taatcccgcc gcccctgacc gccctgccca gaggagcggc tttttattta ttttgtctgc
     6721 gcccgccccg cccgccgccg agagggttgc cgccccaccg ccgaggggag cggtcacccc
     6781 gccgagatgg ttgcgcccgc cgattgcccc gcccgccgcc gaggggatga ccgtcgattc
     6841 ccagcccgcc gttgcgggga gtgccgtccg caaagtcagc cccgataccc ccgcccgccg
     6901 ttgggcgagc cgctttcccc cgaccgtatc caagagaaga caggggattg cccgtcaaaa
     6961 accccaaaca ggcggattaa aattttcttt ttttcgtatt aatcaagctt tgcggaaaac
     7021 aatagacttc ggcggtattt ttggaaaaca agctccccat aaatttcaga gttaaaattt
     7081 tcaaaaaatt tttcaaaaag gtgttgacaa caaaattttt cgtgctattc tataaatagt
     7141 aatagttcct attatcactt aggactcaat aaaacttctt atgcgagtga ttaattatga
     7201 aaaggcacag caagcaaaga gagcttataa tgaaatccct taaaaagctg gaccatccca
     7261 cagcggcgga aatccacgac gatataaagc tcgagtaccc caaaatcagc ctcggcaccg
     7321 tctatcggaa tctgtccgag ctggcggact caaaaatcat actaaggctt ccctccagcg
     7381 acggggctga ccgtttcgac tggaataccg acgagcactt ccacgcccac tgcacagtct
     7441 gcggcaaggt tttcgatttg ttcggtctgc tgcccagaga ggcggtcgac agtcttgaca
     7501 aagcggtgga ggaacatacg gggctcagcg tcagctcaag ggaaatcagc tttaagggaa
     7561 tctgcaaaga ctgcgccaaa aacaaaaact ttcagtaact aaaggcgcga cgcgccggtc
     7621 cataaataaa tcattggagg taatactatg tcacttgtag gcaaaaaggt tagcgatttt
     7681 aaagtaaaag catttcataa cggtgaattc attgatgtaa ccagcgacga cattaagggc
     7741 aaatggtctg tattcgtctt ctatcccgcg gacttcacct tcatctgccc gaccgagctg
     7801 gcagaccttg cggacctcta cgataagttt aaggagatag gctgcgaggt ttactcggta
     7861 tcaaccgaca cccacttcgt tcacaaggca tggcacgacg catccgacac aattaagaaa
     7921 atcaaatatc ccatgctggc agaccccaca ggcaaactct caagggattt cgatgtctat
     7981 cttgaggacc agggtcttgc tctcagaggc agcttcatag tcaaccccaa cggcgagatt
     8041 gtggcttacg aggtcaacgc tgacaatatc ggacgcgacg cctccgagct tttaagaaag
     8101 gttcaggccg ctcagttcgt atgggagcac ggcgacgagg tctgccccgc caagtggaag
     8161 cccggcgaaa agacattaaa gcccagcctg aatttggtag gcaagatata aatgttcagc
     8221 ggagcgacat cccgccacgg gattcgctcc ctttaaatga aggagggaga gcgcatggac
     8281 acgaaaaatc tatatgacct tataatctta ggctcaggca gcgcaggcat gtccgcggga
     8341 atttacgcgg gcagggcaag gctcaaaacc cttgtaatcg ataaggcccg cgcgggtggt
     8401 caaatctcca tcacaaacga ggtagccaac tacccgggcg ttttgaatat atccggcccg
     8461 gagctttccg acaacatgaa aaagcaggct cagagcttcg gcgccaaatt ccttgacgcg
     8521 gaggttacgg cggttgacct tgaaggcgac atcaaaacgg tcagaaccac aaacggtgac
     8581 tacaaagccc tctcggtcat cgccgcaacc ggagcagtcc caagagtcct cggctttaag
     8641 ggcgagcagg agttcagggg caggggcata ggctactgcg ccacatgcga cggcgagttc
     8701 tttacgggaa tggatgtctt cgtcataggc ggcggctatg ctgccgcgga ggaggcggtg
     8761 ttcctgaccc gctacgccaa aaaggtaaga ctcatagtcc gcgagcccga cttcacctgc
     8821 gccagagcgg tcgcggagaa ggctctcaat caccccaaga tagaggtatt cttcaatacc
     8881 gaaatcgtgg aggtcggcgg cgacaatgtg ctgaaatacg ccgagtttgt aaacaatcag
     8941 acgggagaga ggacacgcta cgaggtgccc gagggcgacc ttaccttcgg agtcttcatc
     9001 ttcgcgggct atgtgcccca gtcaaagctc tttgagggcg tactggagct tgacgagttc
     9061 ggctatgtaa agacggacga ggatatgcgc acaaacatcg agggcgtctt tgccgcgggc
     9121 gacatcagac ccaaaaagct caggcagctc gtaaccgccg ttgccgacgg agccatcgcc
     9181 gcaatcgaag cggagaagta tgtaaacgaa aagaagcgtc agctcggcat agccgaaacc
     9241 gaggaaagcg gcgaggagaa cgcagacggc gaattccttg acgacgaaat caaggagcag
     9301 ctcaaagaca tattcgaaag gtttgagaga aaagtcacca tcgccgccgt tcttgagggc
     9361 gacgaggagc tgtcaagcga gcttaaggga ttcctcacgg atttcgccgc cctctccgac
     9421 aaggtggagc tgaagtttat tagcggctca gaattaaagg ctcttggcgc gtctgccgat
     9481 gtccttccca cagccgcagt ccttgacgcc gacggcaagt acacaggaat gcagttcggc
     9541 ggcgtacccg gcggccatga gttcaactcc tttgtaatcg ccctctacaa cgccgcgggc
     9601 cccggacagg aaatctcaga cgaaacaatg tcaaaaatct catccataaa taaaaaggca
     9661 aaccttaaaa tcggcatctc actttcatgc cccctctgcc cagatgtggt ggcggcctct
     9721 cagctcatcg ccttaaagaa caggaatgtg gaggctcaaa tggtggacat ctcaaggttc
     9781 cccgaaatca agaacaagta cgccatcatg agcgtccccg cccttatagt caacgacgag
     9841 aaggtacact tcggaaagaa aagcatagag gaaatcgccg ccatcataat ggcagaaagc
     9901 taaacagcac agcagaagga atgtccgcgc ccaaaaagcg cgggcatttc cttttttatt
     9961 tgtctccggc ggactttgca ccccggcggc aaagttcggc agtatgtcta atcaaggcag
    10021 gtattggcaa tataagggat acattagtat tgacaaaaat tttgcccctt gtattattaa
    10081 tatggttact tgtccaaaca gggcgtcatt ttgtctttta gtgattatac aaggaggtcg
    10141 ccaagatgaa aaaatttctg gcagttttgc tgtcgtttgt cctgattttt acctcggtca
    10201 tcaccgtttt cgcggaggcg ggcgaggagc ccgagttcta cactttgaat ctcgacaaac
    10261 tctatgagga gggagctctc cccgagggcg tgacatttac atatgacgca gggattgacc
    10321 ttaaccgcat tcccgccaat acatatgtaa gccttaaatt caaagtgccc aagcagtaca
    10381 acaccgcaag gcttttcctt aaagtttcgc ccaaccccga gtttgtggac tatgattaca
    10441 ccatactcag gtcttcagcc gacggcacct tccttatcta tatgaattcc gacaagtccc
    10501 ttgcctatga tttgttcgag ataaagagct tctatatcga tgttatagtc atggaggagt
    10561 acgcaatccg ccagtgcgag ccctatgacg agaacgaccc cacccccttt gaggttatcc
    10621 ccggcggcag ggttgttcag tacggcggcg actattggtt taagctcata ctcgctcccg
    10681 actacagcaa cagcgatgtt gtggtcagcg taaacggcaa ggtaattcaa cccgcgggct
    10741 acgacgctga cggcaacagg gtatatcacc ttaagaacat caccgctcag ccggttttca
    10801 gcgttacggg ccttttgccc aacaccgcca acagcatatt cgagtggatt ctgaaaatca
    10861 tgaggctcat cgcgggcatt atcaagggct ggtttaaccg caaccccgag cccaccacca
    10921 cagagccgac cacagagttc accaccgacc ccgaaaaggt atattacagc gcttcaatgc
    10981 cgcccagcca cgagtgggca tacaccgtaa ccccgctgaa taatccctac tatgtgcttg
    11041 agggcagcga ctttaagttc agagtggaca aagcagaggg cgcagtgggc aatatctatg
    11101 tctatgttga cggcgagatt gcagagcccg acgcaagcgg cgtttacacc gtgcgcgagg
    11161 tttccgccga ctttgcggtt gccgttagag caatactctt ctcactgccc gcagagaacg
    11221 cggcatacac ctttactccc gctatcgacc cgacagaggt acagcccggc ggctcattta
    11281 gcttcacagt caccaagagc gagcttgtca caggcaacat cgtggtaaag gccaacggaa
    11341 cggtcatcaa tcccacatca ggcggcgcat acacaatttc caatataaac aaccccgtta
    11401 agattgaggt tcaggttgta tccgttacac tgcccgcttc ggtacccggc atctatgcgg
    11461 taattccgct tacatccgct atcgagattc cgataggcgg cagcttcagc ttcagaattg
    11521 aaaagaacgc aaactacctg ggcaactttg tgataaaggc taacggaacc gtaattaacc
    11581 ccgtcggcgg cgtctatacc gtaaccaata ttacaggtcc cgtaaccatc accattggcg
    11641 cgcaccttgt aaagctgcct cagattgagc cgaatcccta ctacaccgta cagtacctca
    11701 ccgacccgtc aactctgggc gagggcggcg tatacagaat caagataaca aggctcggca
    11761 gcttcaacgg cggtatttcg gtgctcgcca acggcgtcag cgttgacccc tctgagtcag
    11821 acctgacaat ctacgaaatc cccgtaaact cccccgtaga gattgatatt caggcatgta
    11881 cggttctgat taccggactt gacaaccccg taatcagaat gaccgccgtc agcgacctga
    11941 acaacatccc cgtaggcggc agcttctcct tccagcttga gctggtaacc ggctacacgg
    12001 gagagattgt ggtcaaggca aacggcaggg agattgcccc caactccgcg ggcgtataca
    12061 ccgtttccaa tatcagaggc ccggtcacaa tatccatagc ggtatacttc tcgtagcata
    12121 aaaataaagg cgggctgttt gcccgccttt tttccaagaa tacaaaatag gcaggtttga
    12181 tatgaaaatt cctaaactgg cgttatttga ttttggcgga acccttgcgg acgacggcga
    12241 actggatata gactcaggtc ttaaagcagt gctgaacgcc gcgggagtca aagaatatga
    12301 cgaggacctt ctcagaaatt tatggattga tatgcaggag caggtcctga gcgacataag
    12361 aacccccaag ggctacagag tggaaactcc cctttcggca atattcagaa acatcttcgc
    12421 aagagcgggc atcaaagtgg atttgcccct tacggaatgc gaatacatat tcgataagtt
    12481 taactccaca agaaaaagaa ccccctatat agacgagctt ttaaatgtta tgcgcgaaaa
    12541 gggcataaga acgggggtca taagtaatat tgtaatgagc ggcgagtcgc ttaaaatggc
    12601 gattgacgag cttttgcccg acaacaaatt cgagtttatc ctcacaagct gcgactatct
    12661 tttccaaaag cccgcgcccc atatgtttga ggcggcggca aagattgcgg gcgtagagcc
    12721 gcaagactgc tggtatctgg gcgactccta cagagcggat gttctgggcg cctcgcccgt
    12781 tggcatgacc gcgttccgct ataaaaagga gcaggaggaa gaattcctca ctcttaccgc
    12841 agaaattgac ggaaaagaat acacatacta tggtataaac tcttggaaag tccttgctaa
    12901 tatgctaaga gaaaactaat caggggggct gcaaatggaa caggttcgca tcactgagcc
    12961 tacattgctt ctcggagagg acggcaatgt cctaaagccg ggctactgca cgacaaatct
    13021 gtatgtatat aaccgagaga acatcaaggc gcacccgtcg agaatcaagg agtgggactt
    13081 ttaccaaatc tccaacgaca ggtacacggt tcaaatcgtg ttcggcgata tctccttcgc
    13141 gggattcggc tccttcgcat tctttgacag ggaaacgggg gagagggagg acgccctttc
    13201 ggtaataccc ctcaccttcg gcaagatgaa tatgcctcgg acgaccgaga cgcctcacaa
    13261 aatcagcgtc ggctacgccg gcactaagct gcgggttgtt gccgcaaaga ataagcgtta
    13321 cctcaccgcc gacataagag cccgcaggaa cagaataaag gcagacctgg agctggaggt
    13381 catggacggt cttgaatccc ttgtcatggc tgttcccttt gaggacttgc cgggctattt
    13441 ctatctcaac caaaagatga actccatgcc cgtaaaaggc tatgtggaga ttggcagcag
    13501 gatattcgag ttctcgcccc aaaactcctt cgcggttctc gactggggca ggggagtatg
    13561 gccctacaag tgccactggt actggggaaa cggcaccaca aggctggagg acggctccct
    13621 gttcggcttt gaaatcggct ggggcttcgg cgacatgagc gccgccaccg agaatatgct
    13681 tttctacaac ggcaagggac ataagatagg cgaggttcac ctagagaaca ataatcataa
    13741 ggactatatg cagccgtggg tattctcaag cagcgacgga cgctttgaaa tgaccatgac
    13801 accggagtac gacaactaca cctcgtcaag gataggcccc ataggcaaca ggtgccatca
    13861 ggtctacggc aagtggaacg gcacagtggt ccttgacgac ggcaaagagc tgcatattaa
    13921 ggacatgaca gccttctgcg aatactccga caaccgctgg taaaaacgcc ccccatcagg
    13981 gggctttttt atgcccgaac caccgataca aggttatcac cctgccaaac gcgggggatt
    14041 ttttatgcct gctcatttcc gtagccgcca tcccccgccg cagaccgtca caagggtcac
    14101 cgaccgcccc cgctcgcccc gccgccgttg gagtaaccgc cgccccgcaa cacagaggga
    14161 gaacagccct gccagccgac caccccccca acgggggttt tttgcgctgt cgcaaatgcg
    14221 ct
//
LOCUS       DAONVQ010000039        14214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3257_length_14214_cov_4.877039, whole genome shotgun sequence.
ACCESSION   DAONVQ010000039 DAONVQ010000000
VERSION     DAONVQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14214
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3257_length_14214_cov_4.877039"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..1191)
                     /gene="fmt"
                     /locus_tag="PLD98_03860"
     CDS             complement(253..1191)
                     /gene="fmt"
                     /locus_tag="PLD98_03860"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQA05418.1"
                     /translation="MKDVRIVFMGTPDFAVPALKRLYDEGFNVTLVVTQPDKPKGRKR
                     IITPPPVKELALQLGLEVYQPHSLKTDEAVEKISAEKPDFIVVAAYGKILPKSVLDIP
                     KHGCVNIHASLLPRFRGASPIQQAILSGDKETGITTMLMDEGLDTGDILEVVKVEIGE
                     NETAGELFDRLSSLGGDIIVSTLKGILNGTVKPTPQPEEGVTYAPQLTREDGIIDWSM
                     PAEAVHNKIRGLSPWPVAESSLAGKRIKIYSSLKTDIKKDGKGGQIFAEKDRLFVRCG
                     DGQLLEIVTLQPDGSKVMPAADFLRGYQVKEAVFGE"
     gene            complement(1206..1694)
                     /gene="def"
                     /locus_tag="PLD98_03865"
     CDS             complement(1206..1694)
                     /gene="def"
                     /locus_tag="PLD98_03865"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004441495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQA05419.1"
                     /translation="MAIRKIREDSDPILRKKSRPVTEFDERLFTLLDDMAETMYSVDG
                     VGLAAVQVGVLRRVVVMDTGDGLIELINPEIVERGGEQVETEGCLSIPGKRGYTLRPA
                     YVKVRAQNRKGQWCMYKGEGLKARCFCHEIDHLDGILFTDRLAPREKWPKEEKQEELT
                     AK"
     gene            complement(1728..4214)
                     /gene="priA"
                     /locus_tag="PLD98_03870"
     CDS             complement(1728..4214)
                     /gene="priA"
                     /locus_tag="PLD98_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQA05420.1"
                     /translation="MKSAIAAGVAVENTAFDFDKLFHYLIPEDLKDKAVPGVRVIVPF
                     GGGNRMRTGIILSVEDKLSEEAAKNPKSIYAVLDEAPLISNEGISLCRYLKDRTFCTY
                     FEAAKAMLPPSFFMKKNESYILSPDVDESTLSNLSSGEKKVISVLPKNGGYISRDSII
                     KKVGSAKAGGDIDKLAAAGILVPNFSQIERAGAKFQKVISLVLDDDELSEIMPTLTAK
                     QRSVAKVLSDVGSASLKELCEFTSVTPAVVNALVKKGICEESEERIERTVDPDSSNRE
                     GSYKSDPIMLTPAQEKVYNEIKNKLEAKENGVGLLHGVTGSGKTKIYLKLIDDIITEG
                     KTAILMVPEISLTPQTLALLYERYGRKTAVFHSGLSIGERLSEWQRVKNGEATIAVGT
                     RSAVFAPLENIGLIIIDEEQEHTYKSDRSPRYHALDVAKCRAAYHGAYVLLTSATPSV
                     ESYSAAVSGRYDLYELNERYGNAVLPEVETVDMTREQNPLGMTAISERLSQLIAETLK
                     NGNQVILLINRRGYNTFIVCKSCGKVVTCPYCSISLTYHSYNERLMCHYCGYSEPFTA
                     ACKECNAENIRYSGFGTQRVEAELSRLFPDARILRMDADTTAAKNSHKKKLTAFKNKE
                     YDILLGTQMVAKGLDFENVTLVGVISIDQQLYQDDYKSLERAFDLLTQVVGRAGRGEF
                     PGKAVIQTMMPENNIITLAARQDYKAFYNSEIRLRKALIYPPYCDICTVGFTGEDEAL
                     VKNAAKAFFDMLREKNEGEYNDLKLIVLGPMTPRIAKISNKYRYRLIIKCRNTKRFRE
                     FISNLLISFALDKTYSKVRAYADINPTSLI"
     gene            complement(4287..4496)
                     /gene="rpoZ"
                     /locus_tag="PLD98_03875"
     CDS             complement(4287..4496)
                     /gene="rpoZ"
                     /locus_tag="PLD98_03875"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQA05421.1"
                     /translation="MLNPNLKLLLKGNLSRYSLVTATAKRAREIVEEANERKELLTEK
                     PVTLAIEQFLNGEYKIVEPEEIRYI"
     gene            complement(4535..5107)
                     /gene="gmk"
                     /locus_tag="PLD98_03880"
     CDS             complement(4535..5107)
                     /gene="gmk"
                     /locus_tag="PLD98_03880"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQA05422.1"
                     /translation="MNNNGKMGQLVVVSGPSGSGKDTILNEIFKMDPDIIPSVSATTR
                     PPRKGEINGVNYYFMSVEEFEERIKRDEFLEYVQYGDNYYGTLKPQLEEKLKTGKTVV
                     LIIDVRGAVNVRKVYPTAKTIFILPPTESELEKRLRCRESETETSLLKRLEVAKDEIK
                     RVNEYDYAVVNDELEKAVEKVYNIIKSDNF"
     gene            complement(5100..5375)
                     /locus_tag="PLD98_03885"
     CDS             complement(5100..5375)
                     /locus_tag="PLD98_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HQA05423.1"
                     /translation="MKLINIGFGNMLNADRLLAVLSPDSAPIKRIIQESKERGRLIDA
                     SHGRRTRAVLIMDSDHVVLSYLQPETIASRLKSAESEIDAEVESADE"
     gene            complement(5403..6281)
                     /locus_tag="PLD98_03890"
     CDS             complement(5403..6281)
                     /locus_tag="PLD98_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQA05424.1"
                     /translation="MIKSMTGYGRAKQQIGDITVSVEIKSVNHRYFEFSPKLHRNFAF
                     LEDKLKNAVQNKVSRGKIECIVQIDAPEAEEAVVQLNISLAKAYIDAFTKLSEEFGIE
                     NDIKLSDLALKTDIFTVHKEPANEELIWNAVSQVLDEAIDAFVAMRETEGKQLSEDIA
                     SRCDIILKFVEEIDQRSPIVVSEYFEKLAQRIKELLHDAKVDEQRLLTEAAVFADKTA
                     VTEETVRLRSHIDQMKSLLNSCEPVGRKMDFLVQEMNREANTIGSKCQDTAIASLVIN
                     IKAEIEKIREQIQNIE"
     gene            complement(6468..7205)
                     /locus_tag="PLD98_03895"
     CDS             complement(6468..7205)
                     /locus_tag="PLD98_03895"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQA05425.1"
                     /translation="MEKIRLQKFLSDCGVASRRKAEQLIIENRVRVNGVIASIGDKVD
                     PQKDVVTVNGKRVTKPEGYKYIMLNKPRGYISTMSDEFGRKSVADLVKDVGIRVYPIG
                     RLDKDSEGLLLFTNDGEFSNLLMHPSSHVEKTYRVTAKPALKDEDIDRLSLPFMMDGK
                     ETTPAVVTVLEQKEDRTVLEIKLKEGRNRQIRRMLEEIGAETARLKRTAIGGIKLGML
                     RPGKWRELMQEEVASLYKAARTKKGKE"
     gene            complement(7228..8457)
                     /locus_tag="PLD98_03900"
     CDS             complement(7228..8457)
                     /locus_tag="PLD98_03900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HQA05426.1"
                     /translation="MKKKAAIIFTAVFLIVTVIMHDGDLAVITSSFNASSISAKAAVL
                     MCADTGEVLFSLNKDLRLPMASTTKIMTALIALEDPSPMREIKVTKEMVAVEGTSMGL
                     LPDDTISLYTLAAGMLLESGNDAANVTAYAIAGGLNEFAKLMNKKAKELGLKNTNFVT
                     PSGLDADKHYSTAYDLALLGAAAIKNPLFKEICSQKSMKVEFGNPPYPRWLYNHNRLL
                     SSYEGTIGIKTGYTKKSGRCLVSAAERNGITLVAVTLNAPDDWNDHKKMFDYGFSVMK
                     QHDLNDDFSNIYVDVVGGKEKRVAVTTQSKPQAAAANNLNLSKVKREILIKPFEYAPI
                     KSGEILGTVRYYLGDRLLEEQPLCAAADVEIMEYEIKEKERETLFEKLKNFFSGNNKD
                     NKTEKPTKQNKEEFKAE"
     gene            complement(8578..9015)
                     /locus_tag="PLD98_03905"
     CDS             complement(8578..9015)
                     /locus_tag="PLD98_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HQA05427.1"
                     /translation="MKEKTAAGILETTIEKVRNLVNVSTIIGEPIYLNDGITIIPVSK
                     VTYGFASGGSDFPSKNNVQLFGGGGGAGITINPVAFLIVKDGEVTLKHITSSDNAAER
                     IVNLVPEMFDKVTGLVNKSKKKKEEEALDKLEKEIMDEINKAE"
     gene            complement(9075..9815)
                     /locus_tag="PLD98_03910"
     CDS             complement(9075..9815)
                     /locus_tag="PLD98_03910"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HQA05428.1"
                     /translation="MKALAIIIGIIVFFALVLSIRVKVSLDYAEKPACSVRWLFIKIP
                     VYPPKEKTKKPKKEKAKKDKKKKEKAEEPPAEKAPAEKKENPFMVFFKKEGVEGVYSL
                     LSKTVSALNSMIGKIIRQFRIEELYLYMLIGAGDAAETAQKYGETCAKFFPLLGAIVS
                     NFKVKKYDIDISPDFLANKDKAEFHTVISLSPRKLINSVLVFGLKLLFGVLLKFLLKS
                     RPKKNKAAKQNTKTPPAPAKETSEAAAV"
     gene            complement(9843..10475)
                     /gene="scpB"
                     /locus_tag="PLD98_03915"
     CDS             complement(9843..10475)
                     /gene="scpB"
                     /locus_tag="PLD98_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQA05429.1"
                     /translation="MNSAQLKSALEAVLFASGEPIELERLAEAVEAGVDITEQALLEL
                     SAEYEKRNSGIELLRLENKYQLATRPEYADCIKRAMEEKRQAPLSQAALEVLAIIAYN
                     QPVTKSFIEQVRGVDCSGVISSLLRRELIEEAGRLDLPGRPLLYCTTTNFLRTFCISS
                     LDELPPLPENDDAVKIISENLAEMEKAQTEHSETADGGAKDEKEPLALTS"
     gene            complement(10456..11241)
                     /locus_tag="PLD98_03920"
     CDS             complement(10456..11241)
                     /locus_tag="PLD98_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HQA05430.1"
                     /translation="MSTLNPEFKLEVFEGPLDLLLHLITKNKLNIYDIPIITLVEQYT
                     EYIEKCIGEGFDIDSASEFLEMAARLLYIKSAALLPVHEEAEKLKKELTGELIEYQEC
                     KKMAAKLAEMGKGINSYVREPMEIKADMRYKRVHSPKELLEHYLLAAGKKLRRLPPPV
                     DAFRGIVARRIVSVSSKISLIKNLFAKHKRLRLIELYKRSETRSDLVATFLAVLELSR
                     QGSIYITDSMDIVHTPEREKNEPTQLDTTENLGGNKENELSTA"
     gene            complement(11238..11918)
                     /locus_tag="PLD98_03925"
     CDS             complement(11238..11918)
                     /locus_tag="PLD98_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQA05431.1"
                     /translation="MLSQFYKAVQTGDFTTFLIGMSAIIFVLFCTLPVHEFAHAWAAV
                     KLGDETPKNQGRLTLNPLAHLSLLGSLMILVAGFGYAKPVPVNTRNLKNPKKDFALIS
                     LAGPASNLIMAAIFFLLTNITFRFLSPSLLSQVIYYFFYFAATINVNLAVFNLLPIPP
                     LDGSRLWGLVLPDKYYYTVLQYERYIMLALLFLLFTGALTAPLSWLSNLIASAIKFIV
                     GLPFRLLP"
     gene            complement(11931..12815)
                     /gene="prmC"
                     /locus_tag="PLD98_03930"
     CDS             complement(11931..12815)
                     /gene="prmC"
                     /locus_tag="PLD98_03930"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQA05432.1"
                     /translation="MTIGCLLKEGKDFLKSIAGESADFEALCLLEQCLKKDRTYIYLH
                     SDKEADTVIENLYKEMLGRRKNGEPLQYILGEWEFFGRRFKVGKGVLIPRPETEEMVE
                     RALSYIKKLNKPLIVFDLCAGTGCIGISIAKEAPNAKVYLFEKYPDAFYYLKENISLH
                     NAENAVPVLCDVLDGFESLKDIPIPDLIISNPPYIKRDELPGLQTEVQAEPATALDGG
                     EDGLDFYKAFSQKWLPYLNDDGAFIFECAEEQPPIIAEREKNNFFCDTIKDIYGNMRF
                     VCGSRKHENPSKLFSLNI"
     gene            complement(12812..13918)
                     /locus_tag="PLD98_03935"
     CDS             complement(12812..13918)
                     /locus_tag="PLD98_03935"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HQA05433.1"
                     /translation="MKNKKDTACPAANVGGQALLEGIMMNGPLGAAMSVRMPDGRIYT
                     QIKDFVSVKKKYKILNLPIIRGVVTFIESMVFGYKCLMESAELSMTEEELAAESSKLD
                     KWLEKHLGPKLQSVISAVGMVLGVLLSVALFLYLPSLIADLTLGWIDKRLIPLVEGLI
                     RIAVFILYIYLTSKNSYIKRVYMYHGAEHKSIFCYEAGKPLTVENVKGFKRFHPRCGT
                     SFIFVVLIVSILIFTLVLVLFPNIREYRPVWVAIKLLATPLIMGIGYEFIRLAGKYQN
                     PVTKLLSAPGLLMQRLTTLEPTDDIIEVGIAALKAVTDEECCKAAKAAPKEKKTEEPE
                     EPKEKEETPAENAETPQESIEAPESPQENEAESV"
     gene            complement(13908..>14214)
                     /locus_tag="PLD98_03940"
     CDS             complement(13908..>14214)
                     /locus_tag="PLD98_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA05434.1"
                     /translation="KQGDKILVTAEILPRRLKILITDTGCGIPPEALPHVKEKFFKAN
                     VSVRGSGIGLAVSDEIVRLHKGSLNIGSVLNEGTTVEIILPIDTSPPLNERTSEDEK"
BASE COUNT         3160 a         3363 c         3414 g         4277 t
ORIGIN      
        1 gccattcggc tgtaaacggt tctgactctt atgcttgcga tgacggagaa gatgaaagca
       61 ggaagcacta agattaaaag ataggggtca aaatagtagg gtgaatagta gccgtatgag
      121 ccaaaataca aagcctgcac tctcctttcc tgcatttttt gcttgctaag gctattatag
      181 cctataaaaa ttaagttatg attagtaaag cattacccag ttattaatat gcgattaaga
      241 atttatttag atttattctc cgaaaactgc ttctttcact tgatagcctc ttaaaaagtc
      301 cgccgcaggc atcacctttg agccgtcggg ctggagagtg acaatctcta aaagctgtcc
      361 gtcgccgcat cttacaaaaa gcctatcctt ttcggcaaag atttgaccgc ctttgccgtc
      421 tttctttatg tctgttttga gagatgagta aatctttatt ctcttacccg caaggctgct
      481 ttcggcaacg ggccacgggg aaagccctct aattttgttg tgcaccgctt cagcgggcat
      541 tgaccagtcg attatgccgt cctctcttgt aagctgcggt gcgtaggtta cgccctcctc
      601 aggctgggga gtgggcttta ctgtgccgtt taatatgcct ttgagggtgg atacgattat
      661 gtcgccgccc aagcttgaga gtctgtcaaa aagctcgccc gcggtttcgt tctctccgat
      721 ttcgactttt acgacttcga gaatatcgcc cgtgtcaagc ccctcatcca taagcatggt
      781 ggtgatgccc gtttccttgt cgccgcttag tatcgcctgc tgaatgggag aggcgccgcg
      841 gaacctgggc aggagggagg cgtggatatt gacgcagccg tgctttggta tgtcgagaac
      901 cgattttgga agtatttttc cgtatgccgc aacgactata aagtcgggct tttcggcgct
      961 gattttctca acagcttcgt ctgtttttaa agagtgcggc tggtaaacct cgagcccgag
     1021 ctgtaaagcc aattccttta cgggcggcgg ggtgatgatt ctttttctgc ccttgggttt
     1081 atccggctgt gttactacaa gggtaacatt aaaaccctcg tcataaagcc ttttgagcgc
     1141 cggaaccgca aaatcgggcg ttcccataaa aactattcga acatctttca tcggagtcac
     1201 tccaattatt ttgcagtaag ttcttcctgt ttttcttcct tgggccattt ctctctggga
     1261 gcaagtctgt cggtgaaaag tattccgtca agatggtcta tttcgtggca gaaacagcgg
     1321 gctttaagac cctcgccttt atacatgcac cactgaccct tgcggttctg ggccctgacc
     1381 ttaacatagg cgggtctgag tgtatagcct cttttgccgg ggatggaaag gcagccttcg
     1441 gtttctacct gctcgccgcc ccgctccaca atttcggggt ttataagctc aataagaccg
     1501 tcgcctgtat ccattaccac aacgcgcctt aaaacgccga cctgcaccgc cgcaagtcct
     1561 acgccgtcga cgctgtacat ggtttctgcc atatcgtcaa gcagggtgaa cagacgctcg
     1621 tcgaactctg ttaccggcct gctctttttt cttaaaatcg ggtcgctgtc ttctctgatt
     1681 tttctaattg ccatgatggg acattccttt cagtaatgcg aagattctta tatcaaactt
     1741 gtggggttaa tatccgcgta agctctgacc tttgaatagg tcttgtcgag agcgaatgat
     1801 attaaaagat ttgagataaa ctcacggaac ctttttgtgt tcctgcattt tattataagc
     1861 ctgtacctgt atttgttgct aatttttgcg attcttggcg tcatgggtcc gaggacaatc
     1921 agctttaaat cattatattc gccctcgttc ttttctcgca gcatatcaaa gaaagctttt
     1981 gccgcgtttt tcacaagggc ttcgtcctcg ccggtaaagc ctacggtgca aatatcgcag
     2041 tagggcgggt aaatgagagc ctttctgagc cttatttcgg aattataaaa tgccttgtaa
     2101 tcctgccttg cggcaagggt gatgatgttg ttttcgggca tcatggtctg aattaccgcc
     2161 ttgccgggga attctcccct gcccgccctg ccgacgactt gcgtcaaaag gtcaaaggct
     2221 ctttcaaggc ttttgtaatc gtcctgataa agctgctggt ctatggatat aacaccgact
     2281 aaggtgacat tttcaaagtc aagccctttg gccaccatct gggtgccgag cagtatgtcg
     2341 tactccttgt ttttaaaggc tgtgagcttt ttcttgtggg agttcttcgc ggcagtggtg
     2401 tcggcgtcca ttctgaggat tctggcgtcc ggaaagagtc ttgaaagctc cgcctccact
     2461 ctttgggttc caaagcccga atatctgata ttctccgcat tgcactcctt gcatgcggct
     2521 gtaaagggct cggaatagcc gcagtagtgg cacatcagcc tttcgttata gctgtggtaa
     2581 gtcagggaaa tgctgcagta agggcaagtg acaaccttgc cgcaggattt gcagactata
     2641 aatgtattat accctcttct gttgataagc aaaatgactt ggttaccgtt ttttaaagtt
     2701 tcggcaatta attgcgaaag tctttcgctt atggcggtca tgccgagggg gttttgctcc
     2761 cttgtcatat ccaccgtttc gacttcgggc agaacggcgt tgccgtagcg ttcattcagt
     2821 tcgtaaaggt cgtatcttcc cgatacggcg gcggaatagg attcaacgga aggagtggcg
     2881 gatgtaagga gcacatatgc gccgtgataa gcggctcggc actttgccac atcaagggcg
     2941 tgataacggg gcgagcggtc ggatttgtag gtatgctcct gctcctcgtc gataattatc
     3001 agtcctatgt tttcaagggg agcaaagacc gcgcttcttg tgccgacggc gattgttgcc
     3061 tcgccgtttt ttactctttg ccactcactg agcctttcgc cgatggacag accgctgtgg
     3121 aaaaccgctg tttttctgcc gtatctctca tagagaaggg caagggtctg gggcgtgagt
     3181 gagatttcgg gcaccatgag gatggcggtt ttgccctcag tgataatatc gtcaatgagc
     3241 tttagatata tctttgtctt gccgctgccc gtaacgccgt ggaggagccc cacgccattc
     3301 tctttggctt caagtttatt cttaatttca ttgtaaacct tttcctgagc gggcgtgagc
     3361 attatggggt cggatttata gctgccctct ctgtttgagg agtcagggtc tacggttctc
     3421 tcaattcttt cctcgctctc ctcgcagatg ccctttttaa caagggcgtt tacgaccgcg
     3481 ggagtaacgg aggtaaattc gcaaagctct tttaaacttg cgctgcccac atccgagagc
     3541 acttttgcga cgctgcgctg ctttgcggta agggtgggca tgatttcaga aagttcatca
     3601 tcgtcaagaa cgaggcttat aaccttttgg aatttcgccc ccgccctttc gatttgcgag
     3661 aaattcggca caaggatgcc tgccgcggcg agtttgtcga tgtcgccgcc cgccttagcg
     3721 gagccgacct ttttaattat gctgtccctt gatatgtagc cgccgttttt gggcagaacg
     3781 gagattactt tcttttcacc gcttgagagg tttgagagag tgctctcgtc aacatcgggc
     3841 gagaggatgt aggactcgtt ctttttcata aagaacgagg gcggcagcat ggctttcgcc
     3901 gcttcaaaat aggtgcagaa ggttctgtcc ttcagatagc ggcaaaggct aattccctca
     3961 ttagaaataa gcggtgcttc gtcgagcacc gcgtatatgg attttggatt ttttgcggct
     4021 tcttccgaca gcttatcttc gacggacagg ataattccag tcctcattct gttgccgccg
     4081 ccgaaaggaa ctatgactct gacgcccgga acggctttgt cttttaaatc ctcgggaatc
     4141 agatagtgga acagcttgtc aaagtcaaag gcggtattct cgacagcaac tcctgccgct
     4201 atggcagatt tcattgacct tgccatcctt tcgcgttttt ctgctctaat ttaaggcagc
     4261 accagccgta aagctgatgc tttgacttag atgtatctga tttcctcggg ttccactatt
     4321 ttatactcgc cgtttaaaaa ctgctcgata gcgagtgtta cgggcttttc ggtaagcaat
     4381 tcttttctct cgttcgcttc ttcaactatt tctcttgccc tttttgcggt tgcggttaca
     4441 agggaatagc ggctgaggtt gcctttgaga aggagcttta aattaggatt cagcataaaa
     4501 tccccccttg caaatgtgtt tatatatata aaattcagaa attgtcactc ttaatgatat
     4561 tataaacttt ctcgactgct ttttcaagct cgtcgttgac tacggcgtaa tcgtattcgt
     4621 tgacccgctt aatttcatcc tttgcgacct cgagtctttt gagaagcgag gtttcggttt
     4681 ccgactccct gcatctgagc ctcttttcaa gctccgattc ggtgggaggg agaatgaaga
     4741 ttgtctttgc ggtgggatag acctttctta cattgaccgc tcccctgaca tctattatga
     4801 gaactacggt cttgcctgtt ttaagctttt cttcgagctg gggctttaat gtgccgtagt
     4861 aattgtcgcc gtactggaca tattcgagga actcgtccct tttgattctt tcctcaaact
     4921 cctcaacgga cataaagtaa tagtttacgc cgttgatttc gccttttctg ggcggtcttg
     4981 tggtggctga aacagacggg ataatgtcgg ggtccatttt aaagatttcg ttcaggattg
     5041 tgtccttgcc gctgcccgat ggccctgaca caaccacaag ctgccccatt tttccattat
     5101 tattcatcgg cgctttcaac ctccgcatct atttcagact ctgctgattt aaggcgggag
     5161 gcgattgttt cgggctgcag gtagctgagc accacatggt cgctgtccat tatgagcaca
     5221 gcccttgttc ttctgccgtg ggacgcgtca atcagcctgc ccctttcttt gctctcctgt
     5281 atgattcttt ttattggagc cgaatcgggg ctgaggaccg caagcagcct gtcggcattt
     5341 agcatatttc caaagcctat attaatcagc ttcaaaccga acacctctct atgtcgcaaa
     5401 aactactcga tgttttggat ttgctccctt atcttttcta tttccgcctt gatgttgatt
     5461 acaagggacg cgatggcagt gtcctgacac ttggagccta ttgtgttggc ctctctgttc
     5521 atctcctgaa cgagaaagtc catttttctg ccgacgggct cgcaggaatt aagaaggctc
     5581 ttcatctggt ctatatgtga gcgcaggcgg acggtttctt cggtgaccgc ggttttgtcg
     5641 gcaaatacgg ctgcctcggt aagcagtctc tgctcgtcca cctttgcgtc atggagcagc
     5701 tctttgatgc gctgagcaag cttttcaaag tattccgaca cgacaatcgg tgagcgctga
     5761 tcgatttcct cgacaaactt taaaataatg tcgcagcggc tcgctatatc ctcggagagc
     5821 tgctttcctt cggtttctct catcgccaca aaggcgtcaa tcgcctcgtc aagaacctga
     5881 gaaaccgcgt tccagatgag ctcctcgtta gcgggctctt tgtgcaccgt gaaaatatcc
     5941 gtctttaagg caaggtcaga cagctttatg tcgttttcta tgccgaattc ttcggagagc
     6001 tttgtgaaag cgtcaatata ggcttttgcc agtgagatgt tgagctgaac aacagcctct
     6061 tcagcctcgg gggcgtcaat ctgaactata cattctattt tgccccttga aaccttattt
     6121 tgcacagcgt ttttaagttt gtcctcaaga aaggcaaaat tcctgtggag tttggggctg
     6181 aactcaaaat acctgtgatt tacgctcttt atttccacag agacggtaat gtcgcctatc
     6241 tgctgctttg ccctgccgta gcctgtcatg ctttttatca tatagaccta ccattccgca
     6301 ttttgataaa aaagaaaaga taagctgcct ttaactttaa ggcttactga aggcctcaga
     6361 attaaaggca aactgaaaaa ctcacaaagc ggaaaaccgc ttttatatag taaatattat
     6421 agaggcgcgg atattatttg tcaaacattt gtctaagatt ttgcttacta ttccttgccc
     6481 ttttttgtcc ttgccgcctt gtagagtgag gcgacttcct cctgcatcag ctccctccat
     6541 ttgccgggtc tcagcatgcc gagcttaatg ccgcctattg cggtgcgctt gagccttgcg
     6601 gtttcggcgc cgatttcctc aagcatgcgc cttatctgac ggtttctgcc ctctttaagc
     6661 ttaatttcaa ggacggttct gtcttctttc tgctcaagca ccgtgacaac cgcgggagtg
     6721 gtttctttgc cgtccatcat aaaaggcagg gagagtctgt ctatgtcctc gtctttgaga
     6781 gcgggctttg ctgtgaccct gtaggttttt tccacatggg aggaggggtg cattaagaga
     6841 tttgaaaatt cgccgtcgtt tgtaaaaagc agcagcccct cggaatcctt gtcgagtctg
     6901 cctatggggt agactcttat gcctacatct tttacaaggt cggcgacgct ttttctgccg
     6961 aattcgtcgc tcatggtgct tatatatccg cggggcttgt ttagcattat atatttatag
     7021 ccttcgggct ttgttaccct ttttccgtta acggttacca catctttttg agggtcaacc
     7081 ttgtcgccga ttgaagcgat aactccgttg accctgactc tgttttctat tatcaactgc
     7141 tcggcctttc gccttgaagc aacgccgcag tcagataaaa atttctgtag ccttattttt
     7201 tccattcctt atacatcctc aattagtcta ttctgccttg aattcttcct tattttgctt
     7261 ggtgggtttt tcggttttat tatctttatt attgccgcta aagaagtttt tcagcttttc
     7321 aaataaggtt tccctttctt tttcttttat ttcatactcc attatttcaa catcggcggc
     7381 ggcgcaaagg ggctgctctt caaggagcct atcgcccaga taatatctta cggtgccgag
     7441 aatttcgccg ctctttatcg gagcatactc aaagggtttt ataagtatct cgcgcttgac
     7501 ctttgaaaga tttaaattgt tcgcggcggc tgcctggggc ttgctttgag ttgtgacggc
     7561 gactcttttt tccttgccgc ccacgacatc cacataaata ttagagaaat cgtcgtttag
     7621 gtcgtgctgc ttcataaccg aaaagccata gtcgaacatc tttttatggt cgttccagtc
     7681 gtcgggggcg ttgagtgtca cggcgacaag cgtaatgccg tttcgctccg cggcggagac
     7741 aaggcagcga ccgctctttt tggtatatcc cgttttaatg ccgattgtgc cctcatatga
     7801 ggaaagcagc ctgttatgat tgtagagcca tctgggatat gggggattgc cgaactccac
     7861 cttcatgctt ttttgggagc agatttcttt aaacagcggg ttttttatgg cggcagctcc
     7921 caaaagagca aggtcgtagg ctgtggaata gtgcttatcg gcgtcaagac ccgagggagt
     7981 gacaaaattc gtgtttttaa gccccagctc tttcgccttt ttgttcatca gctttgcgaa
     8041 ctcattgagc ccgcccgcaa tcgcgtaggc ggtgacatta gccgcgtcgt tgccggactc
     8101 aaggagcatg cccgctgcca aggtataaag agaaatcgta tcatcgggca aaaggcccat
     8161 tgaggttccc tcaacggcta ccatttcttt ggtaaccttt atctcccgca tgggggaagg
     8221 gtcctcaagg gctatgagcg cggtcattat tttagttgtg cttgccatgg gcagtcttaa
     8281 atctttattt agagaaaaaa gcacctctcc cgtatccgcg cacataagca cggcggcttt
     8341 ggcagagatg gagcttgcgt tgaatgagct tgttattact gcaaggtcac cgtcatgcat
     8401 aataacggtg acgattaaaa aaacagcggt aaagattatt gctgcctttt ttttcataat
     8461 acatcacccg cataggaatt tagcgcggca aagagtcgca ctatacatgc ttatgcgggc
     8521 aatctgttaa aataatacta tgttgataaa aagggatata atccgagaaa attcgactta
     8581 ttcagccttg tttatctcgt ccattatctc cttttccagt ttgtcgagag cctcttcttc
     8641 tttctttttc ttggatttgt ttacaaggcc cgttaccttg tcgaacattt cggggacaag
     8701 gtttactatg cgctcagccg cattgtcgct tgaagtgata tgcttgaggg tgacttcccc
     8761 gtccttgacg ataaggaaag caacagggtt gattgttatg cctgctcctc cgccgcctcc
     8821 gaaaagctga acattgttct ttgaggggaa gtcggagccg ccggaagcaa agccgtaggt
     8881 tacttttgaa accggaatga tggtgattcc gtcgttgaga taaatgggct cgccgataat
     8941 ggtgcttaca tttacaaggt ttcttacctt ttcgattgtg gtttcaagga tacctgctgc
     9001 tgttttttct ttcatggcat tatctttcct ttcctttatt tgaaatggac tgagagccta
     9061 atattttcct tgctttatac cgccgcggct tcacttgttt cctttgcggg ggcaggcggc
     9121 gtttttgtgt tttgctttgc ggctttattc ttttttggcc ttgactttaa aaggaatttc
     9181 agcaatacgc cgaacaggag tttaagtccg aatacgagta cgctgtttat aagctttctg
     9241 gggctaagag atattacggt atggaactct gccttgtcct tgttcgcaag gaaatcggga
     9301 gagatgtcta tatcgtattt tttaaccttg aagttgctga ctatcgcgcc caaaagcggg
     9361 aaaaactttg cgcaggtttc gccgtatttc tgagctgttt ccgccgcgtc gcccgcgccg
     9421 ataagcatat agagatacag ctcctcgatt ctgaactgcc ttataatctt gccaatcatg
     9481 ctgttaaggg cggatacggt tttgctgaga agggaataaa cgccctcaac gccctctttt
     9541 ttaaagaaaa ccatgaaggg gttttctttc ttttccgcgg gagccttttc tgcggggggt
     9601 tcttccgctt tctctttctt tttcttatcc tttttagcct tttccttttt gggcttttta
     9661 gtcttttcct tcggcgggta aacaggaatc tttataaaaa gccaccttac ggagcaagcg
     9721 ggcttttcgg cgtagtcaag ggagactttt accctgattg aaagcacaag agcaaagaag
     9781 acaattattc ctatgataat tgccagtgct ttcatttcta tgcctccctt atgtattctt
     9841 agctagcttg tcagcgcaag gggttctttt tcgtctttcg cgccgccgtc cgctgtttct
     9901 gaatgctctg tttgtgcctt ttccatttcc gcaagattct cggaaataat ctttacggcg
     9961 tcgtcatttt cgggcagcgg cgggagttcg tcaagggaag aaatacaaaa tgttctaagg
    10021 aaatttgtcg tagtacagta taacaaaggt ctgcccggca agtcaagcct gcccgcctcc
    10081 tcaatcagct cgcggcgcaa aagcgaggat atgacgccgg agcagtcgac tcccctgacc
    10141 tgctctataa acgacttggt aaccggctga ttgtacgcga tgatggcaag cacttcgaga
    10201 gccgcttggg acagcggggc ctgtcttttt tcttccatcg ccctttttat gcagtcagcg
    10261 tattcgggtc tggtggcaag ctgatatttg ttttcgagcc ttaaaagctc aattccgctg
    10321 tttctctttt catattccgc cgacagctcc aaaagggctt gttcggttat gtcaacgcct
    10381 gcctctactg cctcagcgag cctttccagt tcaatcggct cgcccgaagc gaagagcacg
    10441 gcctcaagag cgcttttaag ctgtgctgag ttcattttct ttgtttcctc cgaggttttc
    10501 agtggtatca agctgggttg gctcgttttt ctccctttcg ggcgtgtgga caatgtccat
    10561 tgaatcggtt atgtaaatgc tgccctgcct tgagagctcg agaacggcaa ggaaggttgc
    10621 cacaaggtcg gagcgggttt cgctcctttt gtaaagctct ataaggcgca gccttttgtg
    10681 cttagcaaaa agatttttta taaggcttat tttggagctt acggacacta tacgccttgc
    10741 gactatacct ctgaaagcgt cgacgggcgg gggcagacgc cttagcttct tgcccgcggc
    10801 gagaagatag tgctctaaaa gctcctttgg gctgtggact ctcttatagc gcatgtccgc
    10861 cttaatttcc atgggttcgc ggacataact atttataccc ttgcccattt ctgcgagttt
    10921 tgccgccatc ttcttgcatt cctgatactc tataagctca cccgtcagct cctttttaag
    10981 cttttcagcc tcctcatgaa cggggagcaa agcggcggat tttatataca aaagccttgc
    11041 cgccatctct aaaaactcgc tggcggaatc aatgtcaaag ccctcgccga tgcatttttc
    11101 aatatactcg gtgtactgct cgacaagagt tattatggga atatcataga tattcagctt
    11161 gttctttgtg atgaggtgca aaagcaggtc aaggggacct tcaaagacct ccagcttaaa
    11221 ttccggattt aatgtactca tggcaacaac ctaaagggca agcccacaat gaattttata
    11281 gcggacgcga taagatttga aagccagctc agaggagcgg ttaaagctcc cgtaaacaga
    11341 agaaagagta acgccagcat tatatatctc tcatactgaa ggaccgtata gtagtatttg
    11401 tcggggagaa cgagtcccca aagtcttgag ccgtcaaggg gcggtatggg aagaagattg
    11461 aataccgcaa ggtttacatt tatggtagcc gcaaaataga agaagtagta tataacctgc
    11521 gacaaaagcg acggcgataa aaacctaaag gttatgtttg taagaaggaa gaatattgct
    11581 gccattataa ggtttgaggc gggtcccgca agggaaatga gagcgaaatc ctttttgggg
    11641 tttttaagat tccttgtatt cacgggcacg ggctttgcgt agccgaagcc tgccacaaga
    11701 atcataagtg agcccaaaag ggataggtgg gcaagggggt ttaatgtcag tctgccctga
    11761 tttttgggcg tttcatctcc cagtttcacc gccgcccatg cgtgagcgaa ctcatgtacg
    11821 ggcagggtgc agaaaagaac gaatattata gcgctcattc cgataaggaa tgtcgtgaaa
    11881 tctcctgttt ggacagcttt gtagaattgg gaaagcaatt tctcaactcc ttaaatgttt
    11941 agtgaaaaca gctttgaggg attttcgtgt tttctgctgc cgcagacaaa gcgcatattg
    12001 ccgtaaatgt cctttatggt gtcgcagaaa aaattattct tttccctttc ggctattatt
    12061 ggcggctgct cctcggcgca ttcgaaaata aaggcgccgt cgtcatttaa atatggcagc
    12121 catttttgag agaaggcttt atagaaatcc aaaccgtcct ccccgccgtc aagagctgtt
    12181 gcgggttctg cctgcacctc cgtttgaagg ccgggcagct cgtccctttt aatatagggc
    12241 gggttcgaaa tgataaggtc tgggataggt atatctttta aactttcaaa gccgtcaagc
    12301 acatcgcaca aaacggggac ggcgttttcg gcattgtgaa ggctgatgtt ttcttttagg
    12361 taatagaaag catcggggta tttttcaaaa aggtagacct ttgcatttgg ggcttccttt
    12421 gcgatgctga ttcctatgca gcccgttccg gcgcaaaggt caaatacgat gaggggtttg
    12481 tttagcttct taatatagga aagagcccgc tcaaccattt cttccgtttc gggacggggg
    12541 atgagaacgc cttttcctac cttaaagcgc ctgccgaaaa actcccactc gccaaggatg
    12601 tactgcagcg gctcgccgtt tttgcggcgg ccgagcatct ctttgtataa gttttctatg
    12661 acagtgtcgg cttctttatc ggagtgaaga taaatataag tcctgtcttt tttaaggcac
    12721 tgctccaaaa ggcataaagc ctcaaagtcg gcgctctcgc ccgctatgct tttaagaaaa
    12781 tccttgccct cttttaaaag acagccgatt gtcataccga ctctgcctca ttttcctgag
    12841 gggattcggg agcttcgatg ctctcttggg gtgtttctgc gttttctgcg ggtgtttctt
    12901 ccttttcctt aggttcttca ggttcttcgg tctttttctc tttgggagcg gcctttgcgg
    12961 ctttgcagca ctcttcatcc gttaccgcct ttaaagcggc tatgcccacc tcgataatgt
    13021 cgtcagtggg ctccaaggtg gtaagccttt gcattaaaag accgggggcg gacaggagct
    13081 ttgttacggg gttctggtac ttgcccgcaa ggcggatgaa ttcgtagcca atgcccatga
    13141 taaggggcgt cgcgagcagc tttattgcca cccaaacggg tctgtactcg cgtatgtttg
    13201 ggaaaagcac aagcactaaa gtgaaaatga gtatgcttac tataagcacc acgaaaataa
    13261 agctggtgcc gcagcggggg tgaaaacgct taaagccctt tacattttcc acggtgaggg
    13321 gctttcccgc ttcgtagcag aatatgcttt tgtgctctgc tccgtggtac atatatactc
    13381 ttttgatata gctgttcttt gaggttaggt aaatataaag aatgaaaaca gcgattctga
    13441 ttagaccctc taccaatggg atgagccttt tgtcaatcca gcccagggtg aggtccgcga
    13501 ttaaagacgg gagatataaa aagagggcga cggacaaaag aacgccgagt accatgccca
    13561 ctgcggaaat caccgactga agctttggtc ccagatgctt ttcgagccat ttgtccagct
    13621 ttgagctttc agcggcgagc tcctcctcgg tcatggaaag ctccgccgac tccataaggc
    13681 atttgtagcc gaaaaccatt gactcgataa aggttacaac gcctctgatt atgggcaggt
    13741 tcaaaatctt gtactttttc ttgacggaca caaagtcttt aatctgcgta tatattctgc
    13801 cgtcgggcat tctgacggac attgccgcgc ccagcggccc gttcatcatt attccttcca
    13861 aaagagcctg accgcccaca tttgcggcgg ggcaggctgt gtctttttta tttttcatct
    13921 tccgaagtcc tttcatttaa ggggggagaa gtgtctatgg ggagaattat ctccaccgtc
    13981 gtgccttcat tgagcacgct gccgatattt aggctgccct tgtggagcct tactatctcg
    14041 tcggatacgg caagacctat gccggagcct ctgacggata cattggcttt aaagaatttc
    14101 tcctttacat ggggcagagc ctcgggcggg atgccgcagc ccgtatcggt tatgagaatc
    14161 tttaggcggc ggggtaaaat ttccgccgtt accaatatct tatcaccttg cttg
//
LOCUS       DAONVQ010000040        14149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3282_length_14149_cov_5.004967, whole genome shotgun sequence.
ACCESSION   DAONVQ010000040 DAONVQ010000000
VERSION     DAONVQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 14149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14149
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3282_length_14149_cov_5.004967"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /gene="jag"
                     /locus_tag="PLD98_03945"
     CDS             complement(<1..800)
                     /gene="jag"
                     /locus_tag="PLD98_03945"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HQA05435.1"
                     /translation="MIKEAIGTGPTIQAAIEEAHKLLAAPEGTDIQHEVLEMPQKKTF
                     GIFGGSPAKVRAYYEIPDEIESKATVEKAEEKEEVKEQKEKEETEVKGIKALFSLGKK
                     KAADAEKEEAAKEEAEAQSEDKVEKYLSDIIKGMGMGEFKISKSFNGVEYVYDIECED
                     SGALIGRRGETIDAIQYLLRLYANKNGGDNKRVSVNVGDYRKRRADSLTALAQRTARK
                     VLRNGRSITLEPMNPYERRIVHTAVQTVEGVKSHSIGQDDNRRVVISLA"
     gene            complement(867..1847)
                     /locus_tag="PLD98_03950"
     CDS             complement(867..1847)
                     /locus_tag="PLD98_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HQA05436.1"
                     /translation="MNNFLVGIGNIFAAPFGWILAFLYSFTDNYILSLFILTVIFKLI
                     LLPTAIKQQKGAAKQLRLQGKVNKIREKYAGDNRKIQEETAALYQREGYSAMTAGCAP
                     ALISLPIMMGLYGVVYTPISSVLRVSETIINKLAEIAYSISSLNLDPNTVGRQIEIKI
                     LENFNLLLQNGADKVLTPDLISSIQKFNDSFRFMGIYLADSPQGGSFNKLWLIPILAG
                     VTSVASTLFMLIKQKKTNPAMAANPASTGCMALLPVAMSVYFTFLFPAGVGVYWIISN
                     ILQFIQQVILSYTHNPYKLVSAIMVDETVERRSRENNIKRLLSLKEKQSE"
     gene            complement(1865..2086)
                     /gene="yidD"
                     /locus_tag="PLD98_03955"
     CDS             complement(1865..2086)
                     /gene="yidD"
                     /locus_tag="PLD98_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HQA05437.1"
                     /translation="MKKAAISLIEFYQKWISPKLPPRCRYYPSCSQYAKEAIERFGLL
                     KGGALALLRLLRCNPLFPGGYDPVPQKKR"
     gene            complement(2130..2486)
                     /gene="rnpA"
                     /locus_tag="PLD98_03960"
     CDS             complement(2130..2486)
                     /gene="rnpA"
                     /locus_tag="PLD98_03960"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQA05438.1"
                     /translation="MQNFETLKRNSDFRRIYARGKSYGSSSLVTYVLKNTTKNVRIGI
                     TTSKKIGNAVKRNRSRRVILAAFRELSAKVKVGYDFVFVARPLTPNLKSTEIKAVMEK
                     QLKAAGVLLNEPNDGQ"
     gene            complement(2511..2645)
                     /gene="rpmH"
                     /locus_tag="PLD98_03965"
     CDS             complement(2511..2645)
                     /gene="rpmH"
                     /locus_tag="PLD98_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQA05439.1"
                     /translation="MKRTYQPKKRQRKKEHGFRKRMSDRNGRKVLARRRAKGRKRLCA
                     "
     gene            3057..5036
                     /locus_tag="PLD98_03970"
     CDS             3057..5036
                     /locus_tag="PLD98_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQA05440.1"
                     /translation="MKNLVSCPSAKELKYIAKRDYPRLLCRMAAIFLISSILFCALAG
                     QFSVLAASPALLWPVPASSRVTAGWGDGRNHKAIDIAAPRGTPVIAVADGVVTLADNS
                     CTHDEGKTYNCCIAMGKYIKIQHSAKINGKTVVSRYSHLSSIDSKIKKGDFVKAGQVI
                     GYVGSTGYSTGNHLDFKLYFDGREVDPGQYLTIPPDVYYGGSDWSVGGPYVQSLRNRI
                     NHSSKSDLRAEGYTYPISLRPGVSFKIAGAVCSNYNITSLTAGIYDTNGNAYFTKTVK
                     PNAKTYNLSGVDADLKFNKLGTGTYIYELKAQDSSGEEMRILFQHVFTVSNSSTLSAV
                     PGFTVPTALSPGEEFALSGIVASNYNINRFTARLINSSGKAVYEHTAYPNSDRFYLDS
                     LISRFKFASLPEGKYTFRVVAWDKSSSSGKTVINQAVTISSKTAIDGSVYISGLIRMA
                     GREMKADTSDITPEGVKLSYQWKRNGENIPGATKSTYTPTNADVGKAITVVVTAADSK
                     YYGSIESYPVYPLPAASDILPGKYNVDLTTMTVTPAKEGTTVKQLLDGMIESFLYSGV
                     YEGSKKLGSNDILKTGHTLKTHLGLLSFTIVVRGDLDGNGKVNAADARVVLRYVAKLQ
                     NLNKAQIKAADADGNYTVTAADARKILRIAADLDN"
     gene            5049..5840
                     /locus_tag="PLD98_03975"
     CDS             5049..5840
                     /locus_tag="PLD98_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HQA05441.1"
                     /translation="MDKVFENAKCLILDMDGTVYLGNTLIEGSDRFLKEISKKGIDFK
                     FFTNNSSHNAASCVNKLRRLGFDYDESKIIISSYVTIDFIKKNRAGKKVYLLGNDNLT
                     GDFAKAGIPLTTDRDEADIVVLGFDTSITYKKIEDAAYIIARGKEYISTHPDVNCPTD
                     YGFMPDNGSFISLFKTSTGREPDLIMGKPYSHTVDYVCSATGCSKDEIVFIGDRMETD
                     IYIGVKNGVKTALVLSGVTSEESMKTYPFAPTVYAENLEALLKFF"
     gene            complement(5897..6457)
                     /locus_tag="PLD98_03980"
     CDS             complement(5897..6457)
                     /locus_tag="PLD98_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQA05442.1"
                     /translation="MDKDIIRNEVIDTIIKRRTIREYEPRQITEKELNTLLNCAMWAP
                     SGRNSQPVHVRVLQDMDALKLINEDFKNLVGWDTPAYTRYETNPVYHNAPTVFFLFSD
                     RECHMDAGIMTENICIAAESLGLGTCIIGSIGALMNAPEGRVWKKRMNIPEDWHFLIA
                     IAVGYKAENPPPKPRDESHYQVIKKI"
     gene            complement(6481..7527)
                     /locus_tag="PLD98_03985"
     CDS             complement(6481..7527)
                     /locus_tag="PLD98_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05443.1"
                     /translation="MREKTKKAARLIGLLLLASMLMTALSACRIGKEPAVVSVNGAEI
                     DNEIFKYFLSHAYLKADSRANSRAIFTNAAEECVKYVAVNTEFKKRGLVLTSVGKAIM
                     NSEVAPLWSVYSDFYKEIGVSKPTFVKIKQSQAYSDALRTEIFDIGGEKQVSEDLIKS
                     YFAQNYVGFRALAGYFTTVDENGNTVRLPADKIEQIKKNFENAANRINSGSDIDEEAV
                     LMYPGVSDEELGTDFVFLSRSDDGNYPEGFFDKVLELPYETARVLTFEDYIYIVIRSD
                     ITKDSLSYYKENRSDCFKAVTDSMMESEIISWAQDYKAVLNYEAAEKTLEKIAKKIDA
                     YKEKSAEKAKALKA"
     gene            7700..8932
                     /locus_tag="PLD98_03990"
     CDS             7700..8932
                     /locus_tag="PLD98_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA05444.1"
                     /translation="MCDKNFVPAFRFMVVSDVHYKDAHTVERDRFEKAIEIAYRIARN
                     HETYKKLDALFVVGDFADNGSEIQMRAFKDSIDRCVNKDETDVVITLASHEFSQKNGG
                     EEAALERFSKIFNMPYDQHKVINGFHCISLTTTKGCRFDDNKKEFAAKALREAADDDP
                     KKPIFFFQHPHCTDTVYGSICWGEDDLIPILANYPQVINFSGHSHAPINDPRSIHQRY
                     FTALGTGSLSYFELDEFGKVYGTVPPKAENCAQMLIVEADKDNRVRVYPYDVLTDNYF
                     PYVWKIDTPSDPSTFIYTDERYKTDIKPYFTEDARAWAEEIGKDSFVITFDQAKIDKD
                     YVDGYDITVRNKATGAVEKQVSIWSEYYFFDMPKTLSQKIDGLKPDTEYEVEITAESF
                     WLTESDNSLKTEFKTLAE"
     gene            9186..10121
                     /locus_tag="PLD98_03995"
     CDS             9186..10121
                     /locus_tag="PLD98_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HQA05445.1"
                     /translation="MKQVIYIDVLIALNIIIGWLLILAASKILRVTPTPFRLLFASFL
                     SGVYSLLILAPEMMFIISLLIKLILSVSIVAVAFKPTTVRELIRYIAAFFLVNFTFAG
                     IMMAIYFLFKPRGMAFNNGAVYFNFSFTSLLACAAAAYVLVSLFGLYLKRKSADGDLY
                     DVEIKMDGKTVSAKGFMDSGNNLYDSFSGSPVIISDPDFIKPLIPKALHKFFDGDYDT
                     AYLELFPDWKKRVRLIPAISVNGSGLLAAFSPDSVTITSGEKAHTITNIKVAVSREKF
                     SNGEYAVLLNPEVFMGEESRRRLKLSFKAQQEVKT"
     gene            10118..10840
                     /gene="sigE"
                     /locus_tag="PLD98_04000"
     CDS             10118..10840
                     /gene="sigE"
                     /locus_tag="PLD98_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HQA05446.1"
                     /translation="MKIPVKLLLRLRAFKEKMILLFLGDNTFYINGPQTLPPPLCREE
                     EQKCIDRIRNGDESAREPLIVHNLRLVVYIAKKFENSGAGVEDLISIGTIGLIKAVNT
                     FCPDKNIKLATYASRCIENEILMYLRKMGTLKAEISLDEPLNVDWDGNELLLSDVLKN
                     EGDSISADIELEAEKSLLIESVEKLGERERLIINMRFGIDGGREHTQKEVADILGISQ
                     SYISRLEKRIIDQLKKEMERVS"
     gene            10994..11152
                     /locus_tag="PLD98_04005"
     CDS             10994..11152
                     /locus_tag="PLD98_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HQA05447.1"
                     /translation="MKKYVCTVCGFTYDEEKGLPAKGIPAGTKFEDLPEDWVCPVCGA
                     KKSKFKPK"
     gene            11299..12498
                     /locus_tag="PLD98_04010"
     CDS             11299..12498
                     /locus_tag="PLD98_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA05448.1"
                     /translation="MTTKTKRIVALLLAAVMLVTVFAGCNKKPNTDTTGETNANTPTF
                     KGEIVIGTTGPLTGSAASYGNSVKNGAQIAVDEINAAGGVNGYKLVYLFEDDEASGDK
                     AKTAYDKLMDKGMQIFLGAVTSGASISLNDAIKEDGILQLTPSASQLEAVSVNKNAFR
                     LCFTDPIQGQAMAEYAYNTLKLTKAAILFNQDDSYSTGIKEAFEARFKELGGKISVAT
                     SFVEDAQDFSSQLTRIAASDAEFIFMPIYNDKVAQIALGANEKGIKLPMLGCDGWDGV
                     LGKYLKTDEEKKLVEGAIYLTPFVATDPDERIQNFVKKYKEAYDAEPDQFAADGYDGI
                     YVIKAALEKMGLDSEEIDNEKLVQAMIGLEYDGLTGKMKFDENGEPNKSFKYAKIENG
                     TYVAITQ"
     gene            12711..12902
                     /locus_tag="PLD98_04015"
     CDS             12711..12902
                     /locus_tag="PLD98_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05449.1"
                     /translation="MKKYALKLTVLALVATFLFSFAACKKKTDDATTTASAKEHTTEA
                     ATEADTAEETAEETEAETE"
     gene            complement(13012..13806)
                     /locus_tag="PLD98_04020"
     CDS             complement(13012..13806)
                     /locus_tag="PLD98_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQA05450.1"
                     /translation="MNRYNESFLEEIAPNVYKINEFDFDLVFLIVGTEKAMLIDCGTG
                     LADLKSFVRTITDKPLLVVATHGHTDHIGGLLQFEEIYVHKDDMLLTWLSKWLRKQSA
                     GRYPHVLKRHNLSFKNIPKKECRTKFLPLHDGDVFDLGGKTVSVFHTPGHSKGHVVLR
                     LEEDKLIFVGDNIADDLWMHIPFTASLEEWLQSAKRTLELSDGYEVWWSHKSGRLTRH
                     HIEKNIELGEKILKQHKKNDFFKSEKVYKDEESGFKIRYRTDMIFR"
     gene            complement(13936..>14149)
                     /locus_tag="PLD98_04025"
     CDS             complement(13936..>14149)
                     /locus_tag="PLD98_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05451.1"
                     /translation="AVNGKPLNKTDVLQNLIEQSKVGDKLTLTIVRVNRNYSLEKFDV
                     TVTLVEDKGDAQATEETTTSFFFPFD"
BASE COUNT         3687 a         3421 c         3271 g         3770 t
ORIGIN      
        1 gcaagggaga taacaaccct gcggttgtcg tcctgaccga ttgagtggga ttttacgcct
       61 tcaaccgtct gaacggcggt gtggacgatt cttctctcat aggggttcat gggctctaaa
      121 gttatgctcc tgccgtttct taaaaccttt ctcgcggtgc gctgagcaag agccgtaagg
      181 ctgtccgccc ttctctttct gtagtcgccc acattgacgg agactctctt gttgtcgccg
      241 ccgttcttgt tggcgtagag ccttaaaaga tactggatgg cgtctatggt ctctcccctt
      301 ctgcctataa gggcgccgga gtcttcgcac tcgatgtcgt agacatactc aacgccgtta
      361 aagctcttgg agattttaaa ctcgcccatg cccattcctt tgattatatc gctgaggtat
      421 ttctctacct tgtcttcgct ctgagcttcg gcttcttctt ttgctgcttc ttccttctcg
      481 gcatcggcag ctttcttttt gccaagcgag aacaatgcct tgatgccttt tacttctgtt
      541 tcttcttttt ccttttgctc tttaacttct tctttttctt ccgctttttc taccgtagct
      601 ttactttcga tttcgtcagg gatttcgtaa taagccctaa cctttgcggg agaaccgcca
      661 aaaatgccga atgttttctt ctggggcatt tccagaactt catgctgtat gtcagttcct
      721 tcgggagctg caagaagctt gtgcgcttcc tcaatggcgg cctgtatggt cggacctgtc
      781 ccgattgctt ccttaatcaa taatttcacc gcctgtaaaa atattcaaac ttatttgatt
      841 ttattattct ctgattttgg aataaattat tcgctctgtt tttcttttag ggacaataag
      901 cgcttgatat tgttttctct tgagcgacgc tctaccgttt cgtcaaccat aattgccgaa
      961 accagcttgt agggattgtg tgtgtagctc aaaattacct gctgaatgaa ctgaaggatg
     1021 ttggaaataa tccagtacac gccaacgcct gcggggaaga ggaaagtaaa gtaaaccgac
     1081 atggcaacgg ggagaagagc catgcagcct gtggaggcgg ggtttgccgc catggcaggg
     1141 ttggtctttt tctgctttat gagcatgaac agtgttgacg caacagatgt aacgcccgca
     1201 agaatcggta tgagccacag cttgttaaag ctgccgccct gcgggctatc ggcaagatag
     1261 atgcccataa agcggaaact gtcgttaaat ttttggatgc ttgagataag gtcgggtgtt
     1321 aaaaccttgt cggcaccgtt ttgcaaaagc aaattgaagt tttcgagaat cttaatttca
     1381 atctgcctgc ccactgtatt ggggtcgaga ttcaaggagc tgatggagta ggctatttca
     1441 gccagcttat tgatgattgt ttccgatact ctcagaacgc tggaaatggg tgtgtagaca
     1501 actccgtaaa gacccatcat gatggggagg gaaatcagcg cgggggcgca gccggcggtc
     1561 atggcggagt agccctccct ttgataaagg gccgcggttt cttcctgaat ttttctgttg
     1621 tcccctgcgt atttttccct aatcttgttg accttgcctt gcaggcgcag ctgcttggcc
     1681 gctccctttt gctgcttaat ggctgtgggc agcaaaatca gcttaaagat gaccgtaagg
     1741 ataaacagcg agagtatata gttgtcagta aatgaataca gaaaggcgag gatccagcca
     1801 aagggggcgg caaaaatatt gcctatgccg accagaaagt tgttcattgc aatcctccgt
     1861 gattttatcg tttcttttgg ggaacagggt catagccgcc ggggaaaagc ggattgcacc
     1921 ttaaaagcct taaaagggca agtgccccgc cctttaaaag accaaagcgc tctatcgcct
     1981 ctttcgcgta ctgtgagcag gagggatagt acctgcatct gggtgggagt ttgggcgata
     2041 tccacttttg ataaaactca atgagagaaa tggctgcttt tttcattggt ctcccgcggc
     2101 gcttgccgta gttagcgaat gcccttaaat tactgaccgt cgttcggctc gttcagcaat
     2161 acgcctgcag ctttgagctg cttttccata actgctttaa tctctgtgct ctttaggttt
     2221 ggtgtaagtg gtcttgcaac aaatacaaaa tcataaccga ccttaacctt agccgaaagc
     2281 tccctgaacg cagctaaaat cacacgcctt gacctgttgc gctttactgc attgccgatt
     2341 ttcttgctgg ttgtaatgcc gattcttaca ttcttagtag tgttttttaa aacataagtg
     2401 acaagcgaag aactgccgta gcttttgcct cttgcataga ttcttcggaa gtcactgttt
     2461 cgtttaagag tttcaaaatt ttgcaaaaca ctcaccaaat caggttcgca ctaagcgcag
     2521 agtctcttcc taccctttgc tctgcggcgg gcgagaacct tgcggccgtt cctgtcagac
     2581 atcctcttgc ggaagccgtg ctcctttttg cgctgtctct tcttgggctg atatgtcctt
     2641 ttcacgaata accctccttt ttagcccata aggctcatta ttaaagccga ggcttaaagc
     2701 cttcggcgga aaaaccggac cgttttcata tttcaatccc gcagtcagca aagccgattg
     2761 caggctattc cggcaagtat agcagagaac attatataac aatactcaat ttcgtgtcaa
     2821 ccttttttta tttgccagcg ctttctttaa acggcactga cgggaaatta cagcatttaa
     2881 aaacgatgaa ttctttaagt atagtataat ccgaaaattc aatattttca ctttaaattt
     2941 ccttgttaat aaaaggttta ttccccatat tgttgactga attccgctcg gatggtatga
     3001 tttaattgtc gatttattta cctttaaagc agcgaagatt agataggaga tttaagttga
     3061 aaaaccttgt ttcctgcccc tctgctaaag aattaaaata catagcaaag agagattatc
     3121 cgaggctgct ttgccgtatg gcggcaatct ttctgataag ctctatttta ttttgcgctt
     3181 tggcgggaca gttttctgtc ctggcggcct cccctgccct tttatggcct gtgcccgcct
     3241 cctcaagggt tacggcaggc tggggcgacg gcagaaatca caaggcaatc gacatcgccg
     3301 ctccccgcgg aacgcccgtc atagcggttg cggacggcgt tgtaactctg gcggacaatt
     3361 cctgcaccca cgacgagggc aagacataca actgctgcat agccatgggc aaatacataa
     3421 agattcagca cagcgccaag ataaacggaa aaacggttgt ttcgagatat tcccaccttt
     3481 catctataga cagcaaaata aagaagggcg actttgtcaa ggcgggacaa gtaataggct
     3541 atgtgggctc cacgggctac tccacgggca atcaccttga ctttaagctc tattttgacg
     3601 gcagagaggt tgaccccggt cagtacctta ccatcccccc cgatgtctac tatggaggca
     3661 gcgactggag cgtgggcggc ccctatgttc agtccctcag aaacaggata aaccatagct
     3721 ccaaatcgga tctccgagcc gagggctata catatccgat ttccctgcgc cccggagttt
     3781 catttaagat agcgggcgcc gtatgctcaa actacaacat cacaagtctt acggcgggca
     3841 tttacgacac gaacggcaac gcatacttta caaagaccgt aaagccgaac gcgaagacat
     3901 acaatctttc gggcgtggac gccgacctca agttcaacaa gctgggaacg ggcacatata
     3961 tctatgagct gaaggctcag gattcatcgg gagaggaaat gaggatactg ttccagcatg
     4021 ttttcacagt atcaaacagc tccaccctct cagcggtgcc gggctttacg gtgccaacag
     4081 ctctctctcc cggcgaggag ttcgcccttt cgggaatagt ggcttcaaat tacaatataa
     4141 ataggttcac cgcaaggctt ataaattctt cgggcaaggc ggtttatgag catacggcat
     4201 acccaaacag cgacagattc tatcttgact cccttatttc aaggtttaaa ttcgcgtcgc
     4261 ttcccgaagg caaatacacc ttcagagttg tggcgtggga taaatcctca agcagcggca
     4321 agacggttat aaatcaggcc gtaacaatct ccagcaaaac cgccattgac ggcagcgtct
     4381 acatctcggg ccttatcaga atggcgggca gggagatgaa ggctgacacc tccgatatta
     4441 ccccggaggg cgtaaagctc agctaccagt ggaagagaaa cggcgagaat atccccggag
     4501 ccacaaagtc cacatacacc cccaccaacg cggatgtggg caaggcgatt acggtggttg
     4561 taaccgccgc ggacagcaaa tactacggct cgattgaaag ctatcccgtc taccccctcc
     4621 ccgcagcttc ggatattctg ccgggcaagt ataatgtgga tttgaccacc atgaccgtaa
     4681 cccccgcaaa agagggcact acggtaaaac agctccttga cggaatgatt gagagcttcc
     4741 tttattcggg agtttatgaa ggctcaaaga aactcggttc taatgatata ttaaagacgg
     4801 ggcataccct taaaacccat ctgggacttt taagctttac gattgtggtg cggggcgacc
     4861 ttgacggcaa cggcaaagtg aacgccgccg acgcccgcgt ggttttaagg tatgtggcaa
     4921 agctgcagaa ccttaacaaa gcccagataa aggcagcgga cgcggacgga aactacaccg
     4981 tcaccgccgc tgacgcaagg aaaatactga gaatagccgc tgacttagac aattaacggg
     5041 aggaacgcat ggataaagta tttgaaaacg caaaatgcct tatactcgat atggacggta
     5101 cagtgtacct tggcaacact cttattgaag gctccgacag attcttaaag gagatttcaa
     5161 aaaagggcat tgactttaag ttctttacaa acaactcctc ccacaacgcg gcgtcgtgcg
     5221 tgaataagct gagaaggctc ggctttgact atgacgaaag caagattata atctcctctt
     5281 atgtcaccat tgactttata aagaagaaca gagcggggaa aaaggtgtat ctcctcggca
     5341 acgacaatct cacgggggat tttgcaaagg ccggtattcc gctcacgacc gacagggatg
     5401 aggcggacat tgtggttctc ggctttgaca cctccattac ctacaaaaag attgaggacg
     5461 ccgcttacat tatagcaagg ggcaaggagt acatatccac ccaccccgat gtgaactgcc
     5521 ccaccgacta cggctttatg cccgacaacg gctcgtttat ctcccttttc aaaacttcca
     5581 cgggcaggga gccggacctt ataatgggca agccctattc ccatacggtg gactatgtgt
     5641 gctccgccac gggctgctct aaagacgaga tagtctttat aggcgacagg atggaaacgg
     5701 atatttacat tggcgttaaa aacggggtaa agacagccct tgtgctctcg ggagtgacaa
     5761 gtgaggagag catgaagacc tatcccttcg ctcccaccgt ctatgctgag aatcttgagg
     5821 ctctgctaaa gttcttctaa acaaaaccga ataaagatat gaaaaaggca ggagtttttc
     5881 ctgcctttta tgtattttat atcttcttta ttacctggta gtggctttca tccctcggct
     5941 tcggcggagg gttttcggct ttgtagccta ccgctatggc aatgaggaag tgccaatcct
     6001 cggggatgtt cattctcttt ttccagactc tgccctcggg ggcgttcata agagctccga
     6061 tgctgcctat aatgcaggtt ccgagtccca gactctccgc tgcgatgcag atgttctcgg
     6121 tcataattcc cgcgtccatg tgacattcac ggtcggagaa caggaagaac actgtggggg
     6181 cgttgtggta aacggggttg gtttcgtatc tggtgtatgc gggagtgtcc cagcccacaa
     6241 ggtttttaaa gtcctcattt atgagcttta atgcgtccat gtcctgaagc accctaacat
     6301 gaacgggctg agagttcctg ccgctgggag cccacatggc gcagttcaga agggtgttaa
     6361 gctccttctc ggtaatctgc ctcggctcgt actccctgat ggtgcgtctt ttgattatgg
     6421 tgtcaattac ttcgtttctg ataatgtcct tatccataca cctgctcctt tcaccttggt
     6481 ttaagccttt aacgcctttg ctttttcagc gctcttttct ttgtacgcgt ctattttctt
     6541 tgctattttc tccagagtct tttcagccgc ctcatagttc agaaccgcct tgtaatcctg
     6601 agcccaggag atgatttcgc tttccatcat gctgtcggtc acagccttaa agcagtcgct
     6661 gcggttctct ttataatatg aaaggctgtc ctttgtaatg tctgagcgga ttacaatata
     6721 tatgtaatcc tcaaaggtca gcaccctggc ggtttcgtaa ggaagctcca gaaccttgtc
     6781 gaaaaagccc tcggggtaat tgccgtcatc gctgcggctt aaaaacacaa agtcggtgcc
     6841 aagctcctca tcgctgacgc ctgggtacat aagcaccgcc tcctcgtcta tatcggagcc
     6901 gctgttaatg cggttcgcgg cgttttcaaa gttcttcttt atctgctcga ttttatctgc
     6961 ggggagcctt acggtgttgc cgttttcgtc aacggtggtg aaataccccg caagagctct
     7021 aaatcccaca tagttttgag cgaaatagga ctttataagg tcctcggaga cctgcttttc
     7081 tccgccaata tcgaaaattt ctgttctgag ggcgtctgaa taagcctgac tctgctttat
     7141 ctttacaaag gtgggcttag acacgccgat ttccttataa aaatcgctgt aaacgctcca
     7201 aaggggcgcg acctcgctgt tcataatcgc tttgccgacg gaggtcagaa ccagccctct
     7261 ttttttaaac tcggtgttta ccgccacata tttaacgcat tcctcggcgg cgttggtgaa
     7321 aatagccctg ctgttcgccc tgctgtcagc ctttaaatag gcgtgactga ggaaatactt
     7381 aaatatttca ttatctatct cggcgccgtt tacggaaacg acagccggtt cttttcctat
     7441 tcggcaggcg gaaagcgccg tcatcagcat tgatgctagc agtaaaagac caatgagcct
     7501 tgccgccttt ttagtttttt cgcgcattgc tgcctccaag ctaaagttgc tatttttaaa
     7561 ataccataac ccgtatatag tgtaaaccaa attttattaa atgtatagcc gagaataagt
     7621 tttacatctt gtttattgtt ttgcgcccgc caatgtgcta caatgcccaa aagaagtatt
     7681 tagccaaagg agatatatta tgtgcgataa aaactttgtg cccgcttttc gtttcatggt
     7741 agtcagcgat gtccactata aggatgcgca caccgttgag cgtgaccgct ttgagaaggc
     7801 tattgagatt gcctacagaa tagcaaggaa tcacgaaacc tacaaaaagc tggacgcgct
     7861 ttttgttgtg ggcgactttg cggacaacgg ctccgaaatt cagatgagag ctttcaagga
     7921 ctccattgac agatgcgtaa acaaggatga aacagatgtt gtcatcaccc ttgccagcca
     7981 tgagttctcc cagaaaaacg gcggcgagga ggctgctctc gagaggttct caaaaatctt
     8041 caatatgccc tacgaccagc acaaggttat aaacggcttc cactgcattt cccttaccac
     8101 cacaaagggc tgccgctttg acgacaataa gaaggagttc gccgcaaagg ctcttagaga
     8161 ggctgccgac gacgacccga aaaagccgat tttcttcttc cagcaccccc actgcaccga
     8221 caccgtttac ggcagcatct gctggggcga ggacgattta attcctattt tggcaaatta
     8281 cccgcaggta atcaacttct ccggtcactc ccacgctccg attaacgacc cccgctccat
     8341 tcatcaaagg tattttacag ctcttggcac aggctcgctc agctattttg agcttgacga
     8401 gttcggcaag gtttacggca cggttccgcc caaagccgaa aactgcgctc agatgctgat
     8461 agtcgaagct gacaaggaca acagagtaag agtttatcct tatgatgtcc tcaccgacaa
     8521 ttacttcccc tatgtttgga agatagatac cccctccgac ccctccacct tcatttatac
     8581 agatgaaagg tataagaccg acattaagcc ctactttact gaggacgcga gagcctgggc
     8641 tgaggaaatc ggcaaagact cctttgtaat cacctttgac caggctaaga ttgataagga
     8701 ctatgttgac ggctatgaca tcaccgtcag aaacaaagcc accggcgcgg tcgaaaagca
     8761 ggtcagcatt tggtccgagt attacttctt cgatatgccc aaaaccctca gccagaaaat
     8821 cgacggctta aagcccgaca ccgagtatga ggtggaaatt acggcagaga gcttctggct
     8881 taccgaaagc gacaactcct taaagaccga gtttaaaacc ttggcagagt aatattcata
     8941 caaaaaaatt gaagcctttt ttaaggcttc tttttttatt gcccatattt ttatcttttt
     9001 gtctatgttt ttatccgctg ttctcttttt agtcaccgca ttgtgacata ttattaattt
     9061 ccgcaaacat ataattatta gtaaccaagt ttataaacaa gctgtggctt gtatgttcaa
     9121 aacaaaggaa ggaaagacgc gcgaaactgt aaaaagggct gcaacaaagc cgcatggagg
     9181 ctaatatgaa gcaggtaatt tacatagatg tgctgatagc tctgaatata attataggct
     9241 ggctgctgat tctggcggcc tctaagatac tgagagtaac ccctactccg ttcagactcc
     9301 tctttgcctc ttttctttcg ggggtttact ctcttttaat tcttgctccc gaaatgatgt
     9361 ttataatttc gctcttaata aagcttatct tgtccgtcag catagtcgcg gttgccttca
     9421 agcccacgac ggtcagggag ctgataagat acatcgccgc gttttttctt gtcaacttta
     9481 cctttgcggg tattatgatg gcgatttact tcctgtttaa accccgaggc atggctttca
     9541 acaacggcgc ggtttatttt aatttcagct ttaccagcct tcttgcctgc gccgccgcgg
     9601 cctatgtgct tgtaagcctt ttcggacttt acctaaagcg caaaagcgcc gacggcgatt
     9661 tgtacgatgt ggaaataaaa atggacggaa aaacggtgtc cgcaaaagga tttatggaca
     9721 gcggaaacaa cctttatgac agtttttcgg gctcccccgt tatcatctcc gaccccgatt
     9781 ttataaaacc ccttattcca aaggcacttc acaaattctt tgacggtgat tacgacaccg
     9841 cctatcttga gcttttcccc gactggaaaa agagagtcag gctcattccc gccatttccg
     9901 taaacggcag cgggctgctt gccgcattca gccccgactc tgtcaccatt acatcgggtg
     9961 agaaagcgca taccattaca aacatcaagg tcgcggtaag cagagagaag ttttctaacg
    10021 gggaatacgc tgtcctttta aaccctgaag tatttatggg cgaagaaagc cgccgtcggt
    10081 tgaaactcag tttcaaagcg cagcaggagg taaagacatg aagatacccg taaagttatt
    10141 gctgaggctc agagcgttta aggaaaagat gattcttctt ttcctcggtg acaatacctt
    10201 ctacatcaac ggcccgcaga cactcccccc gccgctttgc cgtgaggagg agcagaaatg
    10261 cattgacaga atcagaaacg gcgacgaatc ggcgcgcgag ccgctcattg tgcacaatct
    10321 gcgacttgtg gtctacatag caaaaaagtt cgagaactcc ggcgccgggg tggaggattt
    10381 gatttcaata ggcaccatcg gccttattaa agctgtcaat accttctgcc ccgacaagaa
    10441 cattaagctc gccacctacg cttcaaggtg cattgagaac gagattctga tgtaccttag
    10501 aaaaatggga actttaaagg ccgaaatctc ccttgacgag cccttaaatg tggactggga
    10561 cggaaatgag ctgcttctct cggatgttct taagaacgag ggggacagca taagcgcgga
    10621 cattgagctt gaggcggaaa agagccttct tatagaaagc gtcgaaaagc tgggcgaaag
    10681 agaacggctc attataaata tgcgcttcgg cattgacgga ggcagggagc atacccaaaa
    10741 ggaggtggcg gatattctgg gtatttccca aagctatatc tcaaggctcg aaaaaagaat
    10801 tatagaccag cttaaaaagg agatggagag agtttcgtaa atatatcgtt tttgcgggtt
    10861 gtcaaaacta caagggaaca ccctctcccc ttttgccgca aagacacgcc ctttttccat
    10921 aatttttcat taagtctttc tcttttacca aaaatgtgct atattaattg aaaaaaagga
    10981 aagggtgcaa acaatgaaaa agtatgtatg tacggtttgc ggatttactt atgatgagga
    11041 gaagggtctc cccgcaaaag gcattcccgc gggcacaaag tttgaagatt tgccggagga
    11101 ctgggtatgc cctgtctgcg gcgcgaaaaa gtcaaaattt aagccaaaat aatacggctt
    11161 ttcaggcgaa aacagcactt taagcttgtc gatttaaagc gacaggactt taaagtgata
    11221 gcggaatgga aaaattatgc ttgacaaatc gtgttttttt gattatattc agtacataat
    11281 tccaggaggt gtgaatttat gacaacaaaa accaagagaa tagttgcact ccttctcgcg
    11341 gcagttatgc tcgtaactgt ttttgcagga tgcaacaaga aacccaatac tgacacaacc
    11401 ggtgaaacca atgccaatac tcccacattc aagggcgaaa tagttatagg cacaacaggc
    11461 ccccttaccg gctccgcagc ttcttacggc aactccgtaa agaacggcgc tcagatagct
    11521 gttgacgaaa tcaacgctgc cggcggcgta aacggctaca agctggttta tctctttgag
    11581 gatgacgagg cttcgggaga caaggctaag accgcttacg acaagctgat ggataagggt
    11641 atgcagatat tcctcggcgc tgttacatcc ggcgcgtcaa tctcccttaa cgacgctatt
    11701 aaggaagacg gcattctcca gcttacaccc tccgcttctc agcttgaggc agtttccgtc
    11761 aacaagaacg cattccgcct ctgcttcaca gaccccatac agggccaggc aatggctgag
    11821 tatgcttaca acacacttaa gctgacaaag gccgctatcc tctttaacca ggacgactcc
    11881 tacagcacag gcattaaaga ggctttcgag gcaagattca aggagctggg cggcaagatt
    11941 tccgttgcca catccttcgt tgaagacgct caggacttca gctcacagct tacaagaata
    12001 gccgcttccg acgctgagtt catcttcatg cccatctaca acgacaaggt tgctcagata
    12061 gctctcggcg ctaatgaaaa gggcattaag ctcccgatgc tgggctgcga cggctgggac
    12121 ggcgttctcg gcaagtacct caagaccgat gaggagaaga agcttgttga aggcgctatt
    12181 tacctcactc ccttcgtcgc aacagacccc gacgagagaa ttcagaactt cgtaaagaag
    12241 tataaggaag cttacgacgc agagcccgac cagttcgccg cagacggcta cgacggcatc
    12301 tatgtaatca aggcggctct tgagaagatg ggccttgact ccgaggaaat cgacaatgaa
    12361 aaactcgtac aggcaatgat tggccttgag tatgacggtc tcaccggcaa gatgaagttt
    12421 gacgaaaatg gcgagcccaa caagagcttc aagtacgcaa agatagaaaa cggtacatat
    12481 gtagcaatca cccagtaagc aaaaaggcct gtccgaaagg gcaggccttt aaatctgcga
    12541 atattcttgg acctgcgcta ataaaattta tataacaaat gccaaatttt tggtaacaat
    12601 ctgtaacaat cgcaagattt tattgacaat tctatgtcac tgtgctatca ttctgtacga
    12661 gttgaggcaa atttttcctc aacttatcac tgatgaaagg agtacatatt atgaagaaat
    12721 atgcccttaa gctcacagtt cttgctcttg tagctacatt cctcttctcc ttcgcagcat
    12781 gcaagaagaa gacagacgac gctacaacaa cagcaagcgc aaaagagcat accacagagg
    12841 cagctacaga agctgacacc gctgaagaga ctgctgagga aacagaagca gagaccgaat
    12901 aagatcgcaa gttaacgcta aagttcaggt ttgggctaaa aacttagtta cctcattgcg
    12961 aaattgtact taaaaacctc ccggcaataa accgggaggt tttgttattt attatctgaa
    13021 tatcatatcg gttctgtagc ggattttaaa gcctgattcc tcatccttat atactttttc
    13081 cgatttaaag aaatcgttct ttttgtgctg ttttaggatt ttttcgccca gctctatgtt
    13141 cttttctata tggtgtcttg ttagcctgcc gctcttgtgg ctccaccaga cctcatagcc
    13201 gtcggaaagc tccagtgtgc gcttcgcgct ctgaagccac tcctccaaag aggcggtaaa
    13261 gggaatgtgc atccagaggt cgtcggcaat attgtcgccc acgaaaatga gcttgtcctc
    13321 ctcaagtctt aacaccacat gacccttgct gtgaccgggg gtgtggaaca cactgaccgt
    13381 cttgccgccc aggtcgaaaa catctccgtc gtgaaggggc aggaatttcg ttctgcactc
    13441 ttttttcgga atgtttttaa aggacaggtt atgtcttttg agcacatggg gatacctgcc
    13501 cgcggactgc tttctgagcc attttgaaag ccatgtgagg agcatatcgt ccttatgcac
    13561 ataaatctcc tcaaactgca aaagtccgcc tatgtggtct gtatgaccgt gggttgccac
    13621 aaccagaagg ggcttgtccg taatagttct gacaaagctc tttaaatccg caaggccggt
    13681 tccgcagtct atcagcatcg ccttttcggt gccgacaatg aggaaaacca aatcaaagtc
    13741 aaactcgttt attttgtaaa cattaggcgc aatctcttca agaaaagatt cgttatacct
    13801 gttcattttc tcccccaaag cagtttacaa agtgtcttta ctttgataaa aattatatca
    13861 caaaacccat aagtttcaac aaggcattgg gaaaatttgc agaaaaaatc cgcccctaag
    13921 ggcggatatt gttttttagt cgaaggggaa gaagaaggat gtggttgttt cttcggtggc
    13981 ctgagcgtct cccttatcct ccacaagggt gacggtcaca tcgaattttt caaggctgta
    14041 gttcctgttc accctgacga ttgtgagagt cagcttatcc cctacctttg actgttcaat
    14101 gagattctgc agcacatcgg ttttattgag gggcttgccg ttgacggcg
//
LOCUS       DAONVQ010000041        13967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3341_length_13967_cov_4.160725, whole genome shotgun sequence.
ACCESSION   DAONVQ010000041 DAONVQ010000000
VERSION     DAONVQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13967
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3341_length_13967_cov_4.160725"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PLD98_04030"
     CDS             complement(<1..1254)
                     /locus_tag="PLD98_04030"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05452.1"
                     /translation="MSCFVNLFESNKEYTQILMSIEKGRLPMGVLGLPFSAKAHILNF
                     LAERVGKKALCIVPDESAGRKLTSDIKSLGGKAVFYPAKDFVFIDSATKSRQYEMERL
                     AALSGIVFDHADIIVATADAAMQFTIPKDIFKRKTLTIKIGDELPLNQVIETLLSSGY
                     TRADMVEGPGQFSVRGGILDFYPPGFSDAVRVEFWGDEVDSIAYFDVLSQRRSENLCL
                     VSITPAAEILFPSDEYFADAIEKLAASLKGKKAEKAKDLLLKDADSVKNGIRLGNIDK
                     FIPLAYREKVCLLDYFKDNLLFVCESSAVKEKLDAAVALHHEEVKHFFEEGILCKGLD
                     SFMHDFAYLAAAYERFGAVYLDNFARGSFDTPVKDLVTVNTRQLPVWDGSVKLLLEDI
                     LPMHQRGYTTVILCGTAKAGESLCDE"
     gene            complement(1269..1886)
                     /gene="pth"
                     /locus_tag="PLD98_04035"
     CDS             complement(1269..1886)
                     /gene="pth"
                     /locus_tag="PLD98_04035"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQA05453.1"
                     /translation="MFFKRKNKTEETIAYGPIEYIIAGLGNPGRDYEKTRHNAGFMFA
                     DALAEKHNAVFKPSKFKADTADAMINGVRCILVKPMTYMNNSGEAIRDIANFYKIPSE
                     KVIVVYDDTALPTGKLRIRRKGSDGGHNGIKSIIYHLNSDQFPRIKIGIGSKPHPDFD
                     LKDYVLSVFTKEDLELILPCIDRALEAINIMVTGDIDMAMSKYSG"
     gene            complement(1981..2544)
                     /locus_tag="PLD98_04040"
                     /pseudo
     CDS             complement(1981..2544)
                     /locus_tag="PLD98_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908001.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
     gene            2986..3864
                     /locus_tag="PLD98_04045"
     CDS             2986..3864
                     /locus_tag="PLD98_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQA05454.1"
                     /translation="MKKFTSVALVLVLILSVFAMASCAKKDGDTTTAADTTVPVEAVA
                     KYRILDDGLNEEEYAIGFRKGDQTLRDKVQSILAEMKADGTLGEISTKWFGSDVTTVK
                     TTPTTLNPDAKDDSLKKVLDAGVLVLGLDASFKPMGFTNENGEIVGFDIDVAEEVCKR
                     MGVKLEKKGIEWSTKEKPLNTGIIDCIWNGMSVSEERAKAMNLSEAYMDNKMVFVVPA
                     SSDITSPEQLKGKKVALQSGSTAQEILEGSEYAADMEIVALGTNVEALQQLDLGMVDA
                     VFMDLVVAEYENTQSK"
     gene            4008..4706
                     /locus_tag="PLD98_04050"
     CDS             4008..4706
                     /locus_tag="PLD98_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HQA05455.1"
                     /translation="MTWNDILKATEVMLRYLGPTLKVFFYTLLFSIPLGVVVAVLRKV
                     KFKPVSWLTAVYILIMRGTPLMLQIITVYYAIPMLRINSFPWLQNLLAGIDVRSERYQ
                     FSALIFAFIINYAAYFAEIFRGGIESIPIGQYEAAFSLGYTKSQTFFRIILPQVIKRI
                     VPASSNEIITLVKDTALASVVAYPEIFRIAKEQMNYYSSLVPLFVAGLFYFVMNAILT
                     IVLGSIEKKLDYYK"
     gene            4722..5483
                     /locus_tag="PLD98_04055"
     CDS             4722..5483
                     /locus_tag="PLD98_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HQA05456.1"
                     /translation="MKVLEVNNLSKSFGTLEVIKDISFDVEKGQVISIIGPSGSGKST
                     LLRCINQLERVSGGTIKICGLDMITGYEKGKPAYAEPKTLREIKLKMGLIFQNFNLFP
                     HFSVLKNITDAQIHVLKRSKEEAEEIAMDLLRQMGLPEKADAYPYQLSGGQQQRVSIA
                     RALALNPEILFFDEPTSALDPELTGEILKVIRQLAAKSMTMVIVTHEMAFARDVADTV
                     IFMDQGVIVEQGPAEQVINNPTSERTKLFLNRFEN"
     gene            5775..6443
                     /locus_tag="PLD98_04060"
     CDS             5775..6443
                     /locus_tag="PLD98_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQA05457.1"
                     /translation="MNILDFIFIAVVVLTVFFAAKKGFVLSLIEFVSGLLAIFISYAV
                     APAVAGSIYNSYFKEKVIGILTEKFPALAGGKPLTISISDITNILPKSLSFLAENVGL
                     ESGRIELPNASKYISIEQLEQNLAAPIMKVALTVIAFAILSIVLIIALRIAARLLNKL
                     VKVTPLRSLNTLLGAVFGAVKGILQTGILAAVLSLLGSLTTPSALSGLVENSKICEFV
                     LKLF"
     gene            6468..7124
                     /locus_tag="PLD98_04065"
     CDS             6468..7124
                     /locus_tag="PLD98_04065"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQA05458.1"
                     /translation="MSDSKKKNSDLLTNIWTVPNILTMIRIILVPIFAVLFLKGSYIP
                     ALVVLLLSGLTDFFDGKIARRFNQISELGKILDPIADKFTQITIAVLLFFKFRTASDA
                     SLKAFSWVFLLFLVKEASMLIGGSIMLSKGIRPGASELPGKIATFFFYGAMLVILGFG
                     PEIGAFTKSNPDFAISSTVTMVLVVISAILTFVALFSYFPGLFKQIKEYRESQKTDLN
                     "
     gene            7226..9550
                     /locus_tag="PLD98_04070"
     CDS             7226..9550
                     /locus_tag="PLD98_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HQA05459.1"
                     /translation="MNKLICSKCKIRPAVIFVSKIEGDKTTQEGFCIQCAMKMNIGPI
                     KDIMDKMGIREEDLEEFSQQFSEMFNSVDADSLFEAGGAPTLPLFQGFGVNPVMQDAS
                     GVQASKEEPAGDNKLQKTDNETRDNRRPKNKKNQPKRKFLNVYCTDLTEKARNGQIDR
                     IVGREKEIYRVTQILCRRSKNNPCLIGEPGVGKTAIAEGLALKIASGDVPAKLANKEL
                     HLLDLTALVAGTQFRGQFESRIKGLVDEVRNEGNIILFIDEVHNLVGTGDAEGSMNAA
                     NILKPALSRGEIQVIGATTFNEYRKHIEKDAALERRFQPVKVEEPSINDTYEMLKGIK
                     SYYEDYHKVKISDSLLHKAVVLSERYITDRYLPDKAIDLLDEACTCANLRNKAISEYE
                     LNELEIADLKEQIDFIMSETDSIDYEELARLRSELEKKQLKSEELRVKAQDNAVLEED
                     LAKVISLWLGIPVSKIIESDLKKLANLEEKLKKRIVGQDDAIKAVAAAVRRSRVQINP
                     QRRPASFIFVGPTGVGKTELVKQLANELFDTPETLIRLDMSEFMEKHSVSRIIGSPPG
                     YVGYDEAGQLTEKVRRKPYSVILLDEIEKAHPDVLNILLQILDEGKTSDAQGRVVNFE
                     NTVIIMTSNAGSERKSTSLGFAKDTSTQSREKVLKALSDFLRPEFLGRVDEIVVFDPL
                     GEKEYEKIAEILLGDMVEPLKDKGITLNIGEGVPALIAKNAIKGIRGARDVKNEIRRK
                     IEDKIAGLIIDNADTPITEINITAENGEIVVRGR"
     gene            complement(9594..11132)
                     /locus_tag="PLD98_04075"
     CDS             complement(9594..11132)
                     /locus_tag="PLD98_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA05460.1"
                     /translation="MSEQAVNTTKVLTPTEEEYRNYVYENRRYVGKKETIAYILFDFA
                     QNFTINKYNERFIFDVVRIDFAFQAFTNFVGGIWDVVNDTIIGAIVDKTRTRWGKFKP
                     YLVTLAIPGTIGTLLYWMLPIFFPNTSETHFMKLLTYFLLAITRETIETFRGISVTGM
                     LATISPHPVERTRLITLAHLLSIGDKIPEALMSLLIDGINNGILKFQMKSIYVTMGCF
                     TTLVSGLFALYFSLVAKERIIQSEKGPSVMDGIKSILNNKPILLITLAEFLSSFSINT
                     GMTNYYIDVLGSSSIYMIVGIPGGFVSTPSFALVPWFRRHFSTKVLWLLGSSTGDFLM
                     ALVFLFGSIGGIKNGLYKRRLPMIIAIMIQETLFMFVYGIRKVIPKEMYNEAMDYCEW
                     KNGYRTEGMTSVARGLASKLVNTVGATIRAFLMKKIGYVQGAGHLKQSDKTKYYLFAM
                     CTVIPFVTGSLGMIPKLFYDLSGEKRERMYMELLQRRDEKVRQAGGADAPTLPADAGD
                     TGNC"
     gene            11326..13140
                     /locus_tag="PLD98_04080"
     CDS             11326..13140
                     /locus_tag="PLD98_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HQA05461.1"
                     /translation="MALNISDITDKITAVNETANSFIWGTPLLILLIGTGIFFTLRLK
                     VFQITKAKYIFKETVGSLFEKDSEARKSGKGSISQFQAVSTILAATIGTGNIAGVATA
                     ITAGGPGAVFWMWISAFFGMMTMYAEGVLAVFFRKRNKAGEWTGGTMYTLEEGLKTRG
                     GTKRLAKPLGVMFAVMCVIASFGIGNMTQSNSISDALKTNFGVPLAVSGFAVAFVTGI
                     VIFGGIKKIGAAAEKIIPAASLFYIAAALIIIFKNSQNLQNAALSVLNNAFSLRSIGG
                     GIGGYAVSRALSFGLRRGVFSNEAGLGSSATLHAASDAAEPAVQGMWGIFAVFVDTIL
                     ICSLTAFALLSVNVRALPIDAAAMRITEKGTLVALSTLPENSERDGAMVPLILREPLN
                     DDDAYRKYEGLYGVAYGKKVKIIPSDDFYSSTLYNRYTNVAVLRGIAKVDAKGMKILD
                     GKGRPIIEYAELAPLGGVGLVSTAFSSVLGSAAGKIIAISIFLFAFATLLGWSYNGGK
                     ALEYLLNGKGMTAYKFIFTLFIIIGAVCNLELAWSISDTFNGLMAIPNLIGLILLSGT
                     VVKITENYLKRKSNRQVAEEKPMLSNYADLQAEAEKRV"
     gene            complement(13180..>13967)
                     /locus_tag="PLD98_04085"
     CDS             complement(13180..>13967)
                     /locus_tag="PLD98_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HQA05462.1"
                     /translation="DMPFGFKTGAGVIFGSSGGVSEAVLRFAAASLVKGVKLEFTQLR
                     ALQGVKTTEVKLGDLKLRLAVVSGLANAKRLIEKVRKGEEHYDLIEVMACCGGCVNGG
                     GQPVSENKSAVHDRAMGLYENDRNLEYHSSDENPYLQKIYADEITEELAHELFHTKYK
                     NRRRIASGDLELSKKHESAKIELSICLGERCFERGAQNLYTQLSQYVRDKGIEDLVNF
                     KASFCTEQCSKGPAVSINGKNLYHCTYDKVVEEIEKCLVKANA"
BASE COUNT         3687 a         3203 c         3341 g         3736 t
ORIGIN      
        1 ctcatcgcat aacgactccc ctgcctttgc ggttccgcag agaattaccg tcgtgtagcc
       61 cctctgatgc atggggagga tgtcctccaa aagaagcttt acagagccgt cccaaacggg
      121 aagctgcctt gtgttgacgg ttacaaggtc tttaacgggg gtgtcaaagc tgccccttgc
      181 gaagttgtca aggtaaaccg ctccgaacct ttcgtaagct gcggcaaggt aggcaaaatc
      241 gtgcatgaag ctgtcaagcc ccttgcagag tatgccctcc tcaaagaagt gttttacttc
      301 ctcgtgatga agcgccacag cggcgtcaag cttttccttt accgctgagc tttcgcagac
      361 gaaaagaaga ttgtctttga aataatcaag cagacatact ttttctctat acgcaagtgg
      421 tataaactta tctatattgc cgagtcttat gccgtttttg acgctgtccg cgtcctttaa
      481 aagcagatcc ttcgccttct ctgctttttt gccctttaag gaggcggcga gcttttctat
      541 cgcgtcggca aaatactcat ccgacgggaa aagaatttcc gcggcggggg taatgctgac
      601 aaggcacagg ttttcgctcc ttctttgtga gagcacatca aaataggcta tggagtcgac
      661 ctcgtcgccc cagaactcga ctcttacagc gtcggaaaat ccgggcgggt aaaagtccag
      721 tatgccgcct cttacggaaa actgaccagg accctctacc atatcggctc ttgtatagcc
      781 cgagcttaaa agggtttcaa tcacctgatt taagggcagc tcgtcgccga tttttattgt
      841 gagggtcttt cttttgaata tgtccttagg aattgtgaac tgcattgccg cgtcggctgt
      901 ggcgactata atatccgcat ggtcgaaaac aattcccgac agcgcggcaa ggcgctccat
      961 ttcgtactgc cttgacttgg tggcggagtc aataaaaaca aaatccttgg cgggataaaa
     1021 cactgctttg ccgccgaggg attttatgtc ggaggtaagt tttctgcccg ctgactcgtc
     1081 gggcacgatg caaagcgcct ttttgcctac cctttcggca aggaagttta aaatatgcgc
     1141 ctttgcggaa aagggcagtc ccaaaacgcc catgggaagg cgccccttct caatggacat
     1201 aagaatttgg gtatattctt tattgctttc aaagagattt acaaaacagg acatgcggcc
     1261 tcctgatatc agcccgagta tttggacatc gccatgtcaa tatcgcctgt aaccataata
     1321 tttatggctt ccaatgccct atctatgcac ggcagaataa gctccaaatc ttcttttgta
     1381 aacacgctga ggacatagtc ttttaaatcg aagtccggat ggggctttga gccgatgccg
     1441 atttttattc tcgggaactg gtcgctgttt agatgataga tgatgctctt aattccgtta
     1501 tgcccgccgt cggagccttt tcttctgatt ctgagtttgc cggtcggcag ggctgtgtcg
     1561 tcataaacga cgattacctt ctcggagggg attttataga aatttgcaat atccctaata
     1621 gcctccccgc tgttattcat atatgtcatg ggctttacca atatacacct aacgccgttt
     1681 atcatggcgt cggcggtatc cgccttaaac ttgctgggct taaagacggc gttatgtttc
     1741 tccgcaaggg cgtcggcaaa cataaagccg gcattatgtc ttgttttctc ataatccctc
     1801 ccggggttgc cgagccccgc gattatatat tcgatgggtc cgtaagctat agtttcttct
     1861 gttttattct tccttttaaa aaacataatt cttccctttc agcttttctt tacagcaaag
     1921 aacaggcgag agcgaagcgc cccacccctc tttgagttta tattttcggc aaagccgctt
     1981 ttatctgtcc gctggtctta cccagttttc cttattgacc tgacgctccc ttgcaatggc
     2041 aagcgagttg tcgggcacat cctcggtaat cgttgagcca gccgcggtat aggcgttttt
     2101 gcccaccttg acgggggcga ttagatttgt gttgcagccg ataaacgcgt tgtcctcaac
     2161 tatggtgcgc attttgtttt tgccgtcgta gttgacggtc acgcagccgc agccgaagtt
     2221 tacatttttg cccacatcgg agtcgcccac ataggtaagg tgagaaatct ttgagccgtc
     2281 gcctattacg gagttcttta tctccacaaa attgcccact cttgcgctgt tgcctatgtc
     2341 ggagcccggt ctgagatggg catagggccc gacggttgtg ttgtctccga ttttggagcc
     2401 gaagcactgg gagaagttta ttacggtttt gtttcccacc ttagtgtcgg acaaaacgct
     2461 tgaaggacct ataatgcagc cctcacctat ctctactttt gaaagaatta tggtgttcgg
     2521 cagaattacg gtgccggtgc ctataaccgc gtcatcgctt atgattatcc ccgtaaaatc
     2581 gggaatgata actccctctt ttacaagttc gtttaaatcc tttttgttaa agccgtttac
     2641 aagctcatta aagggtgagc tttcaacagg gctcttcttt ttttcgttag cgctcattat
     2701 cattacctcc cgaaaagaat agcacaaaca aagcctgttc gcaagaatct aaagatttat
     2761 gagttttaga gtttaggctt ttatagcttt ttgagggcaa tgtcataagg tcttgtacct
     2821 ttagtttttg tatattatat caaaatctcc ctaatagtat ttgttaatat ttgtcaaggc
     2881 gtacaataat gaaaaaaatt gaaaaaagac tttactttag tctgctaata tgttagcata
     2941 tcaccaagag gcgagaaagc caacattaca taggaggaaa ttttgatgaa gaaattcaca
     3001 tccgtagcac ttgttcttgt gctgattctt tctgtctttg caatggcttc ctgcgcaaag
     3061 aaagatggcg acaccacaac agccgctgac acaactgttc ccgtagaagc agttgcaaaa
     3121 tacagaattc ttgatgacgg ccttaatgaa gaagaatacg caatcggctt cagaaaaggc
     3181 gaccagactc ttagagacaa ggttcagtcc attcttgccg agatgaaggc agacggaaca
     3241 cttggcgaga tttcaacaaa gtggttcggc tccgatgtta caacagtaaa gaccaccccc
     3301 accacattaa atcccgacgc taaggacgac tcactcaaga aggttcttga cgcaggcgta
     3361 cttgttctcg gtcttgacgc gtcatttaag cccatgggct ttacaaacga gaacggcgaa
     3421 atcgtaggct ttgacattga tgtcgcggaa gaagtctgca agagaatggg cgtaaagctt
     3481 gagaagaagg gcattgaatg gtccaccaag gaaaagcctc tgaacacagg aattattgac
     3541 tgcatctgga acggcatgtc ggtcagcgag gagagagcta aggcaatgaa cctctccgag
     3601 gcttacatgg acaacaagat ggtatttgtt gttcccgctt cttccgacat cacttcaccc
     3661 gagcagctca agggcaagaa ggtagctctc cagagcggct ccacagctca ggaaatcctt
     3721 gaaggcagcg aatacgcagc tgatatggaa atcgtagctc tcggcacaaa tgttgaggct
     3781 ctccagcagc ttgaccttgg aatggttgac gctgtattta tggatctggt agtcgccgaa
     3841 tacgaaaaca cacagtctaa ataaatcctt ccttgcagag ccgggctatt agcccggcat
     3901 ttttttgtcg caaaatcctt gattatttgc atatttaatt gtatcatata ctttagtttg
     3961 ctcatatgct aacatattag ctttattata aacttttgag gtgctgcatg acctggaatg
     4021 acatacttaa agcgacagaa gtaatgctga gatacttagg tccgactctg aaggtattct
     4081 tctacaccct tttgttttca atacctttgg gagtcgtggt tgccgttctc agaaaagtta
     4141 aatttaagcc cgtttcgtgg cttacagccg tatatattct gataatgcgc ggcactcccc
     4201 tgatgcttca gattataacc gtatattacg caatacccat gctgaggata aactccttcc
     4261 cgtggcttca gaacctgctg gcgggcatag atgtaagaag cgaaaggtat cagttctccg
     4321 cgctgatttt tgcctttatt ataaattatg cggcatactt cgcggaaatt ttcagaggcg
     4381 gcatagaatc tataccgatt ggtcagtatg aggccgcctt ctcccttgga tacacaaagt
     4441 cccagacatt tttcagaatt atcttgccgc aggttattaa aagaatcgtc cccgcaagct
     4501 cgaatgagat tattaccctt gttaaagaca cggcgcttgc ctccgtggtt gcgtacccgg
     4561 agatattccg catagcaaaa gagcagatga actattacag ctcgcttgtt ccccttttcg
     4621 ttgcaggtct tttctacttt gtgatgaacg ctatacttac aattgtgctg ggcagcatcg
     4681 aaaagaagct ggattactat aagtaaagcg aggagcgcga aatgaaagta ttagaagtaa
     4741 acaacctttc aaagagcttc ggcacacttg aggtaataaa ggatatatcc ttcgatgtgg
     4801 agaagggtca ggttatctcc ataatcggcc cgtcgggcag cggcaagtcc actttgctgc
     4861 gctgcataaa tcagcttgaa agggtttcgg gcggcaccat aaaaatctgc ggtcttgaca
     4921 tgataacagg ctacgaaaag ggcaagcccg catatgcgga gccgaaaacg ctcagagaaa
     4981 tcaagctgaa aatggggctt atctttcaga attttaatct ctttccccac ttttcggttc
     5041 ttaaaaatat aaccgacgcg caaattcatg tgcttaaaag gtcaaaagaa gaggcggagg
     5101 aaatcgccat ggacctttta aggcagatgg gtcttcccga aaaggcggac gcgtatccct
     5161 atcagctctc tggcggtcag cagcagagag tatccatagc aagggctctt gccttgaacc
     5221 ctgagatact tttctttgat gagcccacaa gcgcgcttga ccccgagctt accggcgaga
     5281 ttctgaaggt cataagacag cttgcggcaa agagcatgac aatggtaata gtcacccatg
     5341 agatggcttt tgcgcgcgat gttgccgata cggtaatctt tatggaccag ggggtcatag
     5401 ttgagcaggg tcccgctgag caggttataa acaaccccac atcagagaga acaaagctct
     5461 tccttaacag atttgaaaat taaaagaaat tgatatttat gacttaaaag actgaagcct
     5521 tatgggcgtc agtcttttct tttacgagtc aaagcaaaat ttaatcaaat cttaatcgga
     5581 tatttatatt tgcttataat aaaaatctta ttatgtaatt gaggataatt ggcttatggc
     5641 ggaaatatat ataagtcaaa gaaaataaaa attcgattag ttttttgttt cttaacggac
     5701 gaatttgtgc tacaattatg gtgaaatgaa atacccatgc gcgggctttc gtttccttaa
     5761 aagggaggga aaatatgaat atactggact ttatttttat tgctgttgtt gtgcttaccg
     5821 tattctttgc cgcaaaaaaa ggctttgtgc tgtcgctcat tgagtttgtt tcaggacttt
     5881 tggcaatttt catctcctac gccgttgccc ctgccgtggc aggttccata tacaactcat
     5941 actttaagga gaaggttata ggcatactca cagagaagtt ccccgctctt gccggcggaa
     6001 agccattaac catttccata agcgacatta caaacatatt gcccaaaagc ctttcatttc
     6061 tggctgaaaa tgttggactc gaaagcggca ggattgagct gcccaacgcc tcaaaataca
     6121 tatccataga gcagcttgag cagaaccttg ccgctcccat tatgaaggta gctttgacgg
     6181 ttatagcctt tgcgattctt tcgattgttc ttattatcgc gctcagaata gccgcaaggc
     6241 ttttaaacaa actggtcaaa gtcaccccgc ttagaagtct aaataccctt ctcggagcgg
     6301 ttttcggagc cgtaaaggga attcttcaga caggcattct cgcggcggtg ctgtcgcttc
     6361 tcggttcgct caccacgccc tccgctctgt cggggcttgt tgaaaactca aagatttgcg
     6421 aattcgtgct gaaactgttt taatttattc tgaaaaggga gatttttatg agtgatagca
     6481 agaagaaaaa cagtgactta cttacaaata tatggacagt gcccaacata ttgactatga
     6541 taaggataat ccttgtcccg attttcgcgg tactgttttt aaagggctcg tatattcccg
     6601 ctctggttgt tcttcttttg tcaggtctta ccgacttttt tgacggcaag attgccagaa
     6661 ggttcaatca gataagcgag ctcggaaaga tacttgaccc catcgcagac aagttcaccc
     6721 agattaccat cgcggttttg cttttcttta aattcagaac cgcaagcgac gcgtcgctca
     6781 aggctttcag ctgggtattc ctcctcttcc ttgtaaaaga ggcgtcaatg cttatcggcg
     6841 gctcgataat gctctccaag ggcatacgcc ccggcgcgtc ggagcttccc ggcaaaatcg
     6901 caaccttctt cttctacggc gcgatgcttg taatacttgg cttcggtccc gaaatcggcg
     6961 cctttacaaa gagcaatccc gactttgcca taagctcaac agttacaatg gttctggttg
     7021 taatttccgc tatacttacc tttgtagcgc tgttcagcta cttccccggc ttatttaagc
     7081 agattaaaga gtatagggag tcccaaaaaa cggacttgaa ctaaataaca atagagtcat
     7141 aatatataag tgatattgat agcattaaaa aaagacaaac atacaataat ttattaagac
     7201 ttttttgctc aattaaggag attcgatgaa taaactaatt tgttccaagt gcaaaatcag
     7261 acctgcagtc attttcgtat cgaaaataga gggcgacaag accactcagg agggtttttg
     7321 catacagtgc gccatgaaaa tgaacatcgg ccccattaag gatatcatgg acaaaatggg
     7381 catcagagag gaggacctgg aggaattcag ccagcagttc agcgagatgt tcaactccgt
     7441 tgacgcggat tcattgttcg aggccggcgg cgcacccacc ctgcctttgt tccaaggttt
     7501 tggcgtaaat cctgttatgc aggacgcatc gggcgttcag gcaagcaaag aggagcccgc
     7561 aggcgacaac aagctgcaaa agaccgataa cgaaacaagg gacaacagaa gaccaaagaa
     7621 taaaaagaac cagcccaagc gcaaattcct gaatgtctac tgcacagacc ttaccgaaaa
     7681 ggcaaggaac ggtcagattg acagaatcgt tggcagagaa aaggagattt acagggtaac
     7741 tcagattctt tgccgccgtt caaagaacaa cccctgcctt atcggtgagc ccggcgttgg
     7801 taagacggcc attgccgaag gtctggcttt aaagattgca tcgggcgatg tccccgcaaa
     7861 gctcgcaaac aaagagctgc accttcttga ccttaccgct ttagtggctg gcacacagtt
     7921 cagaggtcag tttgaaagca gaataaaggg tcttgtggat gaggttcgca acgaaggcaa
     7981 tataattctc ttcatcgacg aggttcacaa tctcgtaggc acaggcgacg ccgaaggctc
     8041 gatgaacgcc gcgaatatct taaagcccgc tctttcaagg ggcgaaattc aggttattgg
     8101 cgcaacaacc ttcaatgagt acagaaagca tattgaaaag gacgccgctc tcgaaaggcg
     8161 tttccagccc gttaaggtcg aagagccctc aataaacgac acatacgaaa tgctcaaagg
     8221 cataaagtct tattacgagg attatcacaa ggtcaaaatc agcgactcgc ttttacacaa
     8281 ggcggtcgtt ctctccgaaa ggtatatcac cgacaggtat ctgcccgaca aggctattga
     8341 ccttttggac gaggcttgca cctgcgcaaa tcttcgcaat aaggctataa gcgaatatga
     8401 gctcaatgag cttgaaatcg ccgatttaaa agagcagatt gactttataa tgtccgaaac
     8461 ggattcgata gactatgagg agctggcaag gctgcgctca gagcttgaga aaaagcagct
     8521 aaaaagcgag gagctgagag taaaagctca ggacaacgcc gttctcgaag aggatttggc
     8581 taaggtcatt agcctctggc tcggcatacc cgtaagcaag attattgaaa gcgacctcaa
     8641 gaaacttgcc aaccttgagg aaaagctgaa aaagaggatt gtgggtcagg acgacgccat
     8701 taaagcggtt gcggcagctg ttcgccgcag cagggttcag ataaatccgc aaagacgccc
     8761 cgcgtcgttt atcttcgtag gtcccacggg cgtcggcaaa acagagcttg taaagcagct
     8821 tgccaatgag ctgtttgata ctcccgaaac gctgattagg ctcgatatgt ccgagtttat
     8881 ggaaaagcac agcgtttcaa ggattatcgg ctcacctccc ggctatgtgg gctatgacga
     8941 ggcgggacag cttaccgaaa aggtacgccg caagccttac agcgttatac ttcttgacga
     9001 gatagaaaag gctcacccgg atgtgctgaa catcctcctg caaattcttg atgaaggcaa
     9061 gaccagcgac gctcagggca gagtcgtaaa ctttgagaat acggtcatca taatgacctc
     9121 caacgccggc agcgagcgaa agagcacatc tctcggcttt gccaaggata ccagcaccca
     9181 gtcaagggaa aaggttctga aggctctctc cgatttcctg cgccccgaat ttttgggcag
     9241 agtcgatgaa atagtcgtct ttgacccgct gggagaaaag gaatacgaaa agattgccga
     9301 aatcctcctt ggcgacatgg tagagccttt gaaagacaag ggcattactc ttaacatcgg
     9361 cgaaggagtg cctgccctga tagctaagaa cgccatcaag ggcataagag gcgcaagaga
     9421 tgtcaagaat gaaatccgcc gcaagataga agacaaaatc gcggggttaa taattgacaa
     9481 cgccgacact ccgattactg agattaatat cactgccgaa aacggcgaaa tcgtcgtaag
     9541 gggcagataa aaaacaaaaa ttaaccaccc tcttttggag ggtggttttt ttattagcaa
     9601 ttgcctgtgt ctccggcatc tgcgggtagg gtcggggcgt ctgcgccgcc tgcctgcctt
     9661 accttttcat ctctgcgctg cagaagctcc atatacattc tttctctctt ctcgccggag
     9721 agatcgtaga agagcttcgg aatcatgccg aggctgcctg ttacaaaggg aattactgtg
     9781 cacatcgcga acagatagta ctttgttttg tcggactgct ttaagtgtcc tgcgccctga
     9841 acatagccga ttttcttcat aaggaaggct ctgattgtgg ctcccacggt gttaaccagc
     9901 ttgcttgcaa gtccccttgc gacgctggtc atgccctccg tgcggtagcc gttcttccat
     9961 tcgcagtagt ccatcgcctc gttgtacatt tccttgggga tgaccttgcg tatgccgtaa
    10021 acgaacataa acaatgtttc ctgtatcatt atggcgatta tcataggaag acggcgcttg
    10081 taaagaccgt tctttatgcc gcctatggag ccgaagagga atacaagagc catgaggaag
    10141 tcgcccgtgc tggagcctaa gagccacaga acttttgtgg agaaatgtct tctgaaccag
    10201 ggaaccaatg caaagctggg ggttgaaaca aagccgccgg gtatgccgac tatcatataa
    10261 attgaggatg agccgagaac atctatgtag tagttcgtca tgcccgtatt gatggagaag
    10321 gagcttaaaa actccgcaag ggttataagc agtatgggct tgttgttaag aatcgacttg
    10381 attccgtcca ttacgctggg gcccttttcg gactggataa ttctctcttt tgctacaagc
    10441 gagaagtaca aagcgaacag acccgaaacc aaggtggtaa agcagcccat tgtgacataa
    10501 atgctcttca tctggaactt aagtatgccg ttgttgattc cgtcaatcaa tagggacatc
    10561 agcgcttcgg ggattttgtc gcctatggag agaaggtgcg ccaaggtaat tagccttgtt
    10621 ctttccacag ggtgcggcga tattgtcgcc agcatgcccg ttacggaaat gcctctgaaa
    10681 gtctcaatcg tctccctggt gatagctagc aagaagtatg ttaggagctt cataaagtgg
    10741 gtttcggagg tattggggaa gaatatgggc agcatccagt aaagcagggt gcctatggtg
    10801 ccgggaatcg caagagttac aagatagggc ttgaacttac cccatcttgt tcttgtcttg
    10861 tcaactatgg cgcctatgat tgtgtcattg acgacatccc agattccgcc cacaaaatta
    10921 gtaaatgcct gaaaggcaaa atcgattctt acgacatcga atataaatct ctcgttgtat
    10981 ttgttaatcg taaagttctg ggcaaagtca aagagtatgt aggctattgt ttcctttttg
    11041 cccacatatc tgcggttttc gtacacatag ttgcgatatt cttcttcggt tggggttaaa
    11101 actttggtgg tgtttactgc ttgttcagac aaaggcttca ccctttcggc ggagaataat
    11161 actcctaaaa cgcttatatg gtaagtctat cacaaaaaaa atataattac aacagtattt
    11221 tatacaaaaa caacactgta aatttttgca tttgatactt ttgtggataa gccgttatct
    11281 tctgctggcc tcacgaatag tatttatagg acaggaggga tgtacttggc tttaaatatt
    11341 tcggatataa cagataaaat caccgcggtg aatgagaccg caaactcttt tatctggggc
    11401 actcccctgc ttatacttct tatcggaaca ggcatatttt ttacgctgag acttaaggtt
    11461 tttcagataa caaaggcaaa gtacatattt aaagaaaccg tcggctcatt gtttgaaaag
    11521 gacagtgagg ctagaaaaag cggcaaaggg tctatttcgc agtttcaggc tgtttccact
    11581 atactggccg caacgatcgg aacgggcaac attgcgggag tcgccacagc cataaccgcg
    11641 ggcggcccgg gagcagtttt ctggatgtgg atttccgcct tctttggaat gatgactatg
    11701 tacgctgagg gcgtacttgc ggtttttttc aggaaaagaa acaaagcggg tgaatggacg
    11761 ggcggcacca tgtataccct tgaggagggg ctgaaaacaa gaggaggaac taagcggctc
    11821 gccaaacccc ttggcgttat gttcgctgtg atgtgcgtaa ttgcctcttt tgggataggc
    11881 aatatgacac agtccaactc gatttcggac gctttaaaga ccaatttcgg cgttcctctt
    11941 gccgtttcgg gctttgcggt tgcatttgtt acgggcattg tgattttcgg cggaattaag
    12001 aaaataggag ccgccgcgga aaaaataatc cccgccgctt ctctttttta tattgcggct
    12061 gcgctgatta taatattcaa aaactctcaa aaccttcaaa acgccgctct gtcggtgctc
    12121 aataacgcat tttcattaag gtccataggc ggcggaatcg gcggctacgc tgtttcaagg
    12181 gctctttctt tcgggcttag aaggggcgtt ttttcaaatg aggcgggact cggatcctcc
    12241 gctacgcttc acgccgcctc tgacgcggca gagcccgctg ttcagggcat gtggggaatc
    12301 tttgcggtgt ttgtggatac catactcatc tgctcactta ccgcctttgc gctgctttct
    12361 gtcaatgtca gagccctgcc catagatgcc gccgcaatgc gcattacaga gaagggcaca
    12421 ttggtggcac tctctacatt gcccgaaaac tcggagcggg atggggcgat ggtgcctctg
    12481 atactcagag agcctttgaa tgacgatgac gcttacagaa aatatgaggg gctatacggc
    12541 gttgcctacg gcaaaaaggt aaagataata ccaagcgatg atttttactc ctcaactcta
    12601 tataaccgct acacaaatgt cgcggttttg aggggtatag caaaagtcga cgctaaggga
    12661 atgaaaattc ttgacggcaa aggcagaccg ataattgaat acgcagagct tgcgccattg
    12721 ggcggcgtgg gacttgtcag caccgcgttt tcatctgtac tgggcagcgc ggcggggaaa
    12781 atcatcgcaa tatcaatttt cctctttgcc tttgctactc tcctcggctg gtcatacaac
    12841 ggcggcaagg ctttggaata tcttttaaac ggcaagggta tgaccgcata taagtttatt
    12901 ttcacgctgt ttataataat cggcgccgtg tgcaatctgg agctggcgtg gagcatatcc
    12961 gacaccttca acggacttat ggcaattcca aaccttattg gacttattct cctgtcgggt
    13021 acggtggtta agataaccga aaattactta aagagaaagt ctaacaggca ggtcgcggaa
    13081 gaaaagccaa tgctctcaaa ctatgctgat ttgcaagcag aggcggaaaa aagggtataa
    13141 aaaagagccg aaactttcgt ttcggctctg tgtttttaat taggcgtttg ccttaacaag
    13201 gcacttttcg atttcctcaa caaccttgtc atatgtgcag tgatagaggt tcttgccgtt
    13261 aatgcttact gcgggaccct tgctgcactg ctcggtgcag aaggaagcct tgaagtttac
    13321 aagatcctcg atgcccttgt cacggacata ctgtgaaagc tgggtgtaga ggttctgagc
    13381 gcctctctcg aagcatcttt cgcccaggca gattgaaagc tcaatctttg cggactcgtg
    13441 ctttttggag agttcaaggt cgccggaagc aattctgcgc ctgttcttat acttggtgtg
    13501 gaagagctcg tgggcaagct cctctgtgat ttcgtctgcg taaatcttct ggagataggg
    13561 gttctcgtcg gagctgtggt actctagatt tctgtcgttc tcgtaaagac ccattgctct
    13621 gtcgtgaaca gcggatttgt tctcggatac gggctggccg ccgccgttta cgcagccgcc
    13681 gcagcaggcc ataacctcga taaggtcata gtgctcttcg ccttttctga ccttttcaat
    13741 gagcctcttg gcgtttgcaa gtccgctgac aacagccaga cggagcttaa ggtcgccgag
    13801 ttttacctct gtagtcttaa cgccctggag agctctgagc tgtgtgaact caagctttac
    13861 gccctttaca aggcttgctg ccgcaaagcg gagaactgcc tcgcttacgc cgccggagct
    13921 gccgaagatg acgcctgcgc ctgtcttgaa gccgaagggc atatcaa
//
LOCUS       DAONVQ010000042        13573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3466_length_13573_cov_5.559402, whole genome shotgun sequence.
ACCESSION   DAONVQ010000042 DAONVQ010000000
VERSION     DAONVQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13573
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3466_length_13573_cov_5.559402"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="PLD98_04090"
     CDS             complement(<1..1350)
                     /locus_tag="PLD98_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQA05463.1"
                     /translation="MNSDELRNNENENSQRADENNTENNTNPINPSSGFIYSSGGNSE
                     PQPQNNTQNQSGGYQNSSAPNQGGYQRPAGYQGHPFGSPYYQGQPQGQGSSQYPYGQG
                     RGYYAPPTKPPKKSKGKTVFGVIIGIVVLAIVIYGFYPIFTSVRSENTTAPSEQASTI
                     EGANSAPTLNLNDSQIPKTDNPVGAKELSATEVYKKIKESAVGILVYASDGSIYTEGS
                     GVIMGEDSEGKYTYILTCAHVISSRNSISVQLYDGTDYVAEIVGYDKRTDIGVLRIKA
                     KGLKAAEFGNSEQLEVGQKVYAIGNPGGTEFAGSFTDGIISAIDRPINSSSTGYTMIC
                     IQHTAAINPGNSGGALVNSLGQVIGINSMKIVSTDYEGMGFAVPSKIFQEIFNQIVKN
                     GYVPNRPKLGIKYLPASSYQNLSIVVQLKELPAGTIVIEEISPDSSLYNSKVQVKDLI
                     "
     gene            complement(1526..3061)
                     /locus_tag="PLD98_04095"
     CDS             complement(1526..3061)
                     /locus_tag="PLD98_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQA05464.1"
                     /translation="MKNGLKPISLMQNFMQKGSFFKRLFFVLAFVILSSLVFLGLLMM
                     IFVSGFWSRECIESLQSDAISMASFVGSFYESGKLDDLNSPEGENSAILICSALSRLS
                     ESSNSTIFICDLEGKPILSKEMLSDDMTEIVSEPSTNGIIVTRELIESIGPEGFGGAG
                     RYSSSIEKYFVGAMPVYSGNSLKAIVIAAQPVGIAFRPYFLSVLYIFLIAAFCALIVG
                     LVSVYIISDSVTKPLNLMSQAIKQYAEGNFAYRLPVRGDDEISTLCASINYMAGALAA
                     LEESRRSFVANVSHELKTPMTSIAGFIDGILDGTIDEKNQKHYLRIVSAEVNRLSRLV
                     TSMLNLSKIETGEVQLKPKTFNIGEQVLKAMLTFEKPISDKRINVTGLDKIGSVYVNA
                     DEDMIYQVIFNLIDNAVKFTNEGGTISVSIFERNDGIELSVRNTGSYIPKEEIGNIFD
                     RFYKVDKSRSLDAKSAGLGLYIVKSIVEMHGGNIKVTSIKDQYTEFLVFIPSNNTEPL
                     GYN"
     gene            complement(3134..3838)
                     /locus_tag="PLD98_04100"
     CDS             complement(3134..3838)
                     /locus_tag="PLD98_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQA05465.1"
                     /translation="MANEKIMIVDDDVNICELLRLYLEKEGFTTVILNNGQDAVESFN
                     SVKPDLVLLDIMLPGLDGWQVCRKIRSVSSTPVIMLTAKGETFDKVLGLELGADDYIV
                     KPFDTKEVLARIKAVLRRVSTQTPEQIKEVRYDKLVINLTNYELRVNGVQIDTPPKEL
                     ELIYHLASNPNRVFTRDQLLDEVWGFDYYGDSRTVDVHVKRLREKLEGVSDQWELKTV
                     WSVGYKFETKEPPITG"
     gene            complement(4004..6052)
                     /locus_tag="PLD98_04105"
     CDS             complement(4004..6052)
                     /locus_tag="PLD98_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05466.1"
                     /translation="MKSTRKSALTLLSVLILTAMLASNLLASAAIMFVETQFTYDQPD
                     TAWLKDLIIKEDVLTVQGLSERVKLIAQPDYPYSETPESFAQVVGYYLKFYELDPGSQ
                     EAAYLYIMEQLNKFSAATELNVSDEYIKFWLQSSGIKYPENGLKDNTTLILARTLYAA
                     MSRSNLGVSSFPKGTSLESAAIQIIAKVFGMDISLLSQWLDDVSVDNLESYIIATSKI
                     ALYTMGYDVSKSTPKEEVYRLMAVMTIEKQGISIDAKNASFEEIKIKYLAAMLGRQFG
                     VALDPAELQKAIANDRVPLYILQVMGKTANLTIRSDSNYADAFIAVAQNTHFFDLDEN
                     EFYCDIYNYKGQLKYKRSSVWINPAALYKDGQGTKVVITANGAPIKDNYYSEVPIDPN
                     QKEQVIQIKVTYTKDGQTNSKTYNITIINGTQLPPTPGSGKGDNQTIDDIIENLIPKT
                     EDVTEALDSLLGNLPGRIKDISSLLVPDFSFSDISFDSISGFFSDFFNAGSQVLKGSV
                     LGGIGGLDLLRAEKLPGFGALKDSKLFSTVSQEAANLLELSFLPDSEDSKSGSPVLHN
                     APDIVDVGYYEYKEPEEITQPAVAVNVVPYQPVESLASPPKGYEYVTDANGYAVGLAV
                     SNGGRIVFENDGSASFIKKNMVYILLPITAAAAVVVTVLAKKKQKVEIIPEEILNFEE
                     "
     gene            complement(6446..7135)
                     /locus_tag="PLD98_04110"
     CDS             complement(6446..7135)
                     /locus_tag="PLD98_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HQA05467.1"
                     /translation="MKLTKKVLVGLVVLALMASMALSAFAAAPALAIKVGEPNDKGEV
                     KVDVILKNAAGLQSGGFYLEFDADAYTFVKSAAGVDARNSATNVVTDLVPGTSTVTAA
                     VANADKVVASDDEDEPVNVDNFVIFTVTFKVKDAAKAKAAAFKAGTIDGEPVQINDAD
                     VNLAAVTYQPKVEETTKAPATTKAPATTTTKPAGSIPKTGLSSGDAFASAAIATLAVA
                     AAAFVVTKKKK"
     gene            7744..9435
                     /locus_tag="PLD98_04115"
     CDS             7744..9435
                     /locus_tag="PLD98_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005539819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQA05468.1"
                     /translation="MRENWLCLNGKYEYAVTRNINTFPKSFDGEIIVPFAIESELSGV
                     GRKLLPDERLWYRRYFTVPENFKGKRIILHFDAVDWQCHVYVNRRPATIHKGGYSHFS
                     IDITELLKDGENELIVRVYDPSDTGLQQRGKQRLKSVGIWYTATSGIWQPVWLEAVED
                     ISIENIRLTPDFDGGKIRIESTVKGKAEKDKLRLELKVFESIDGGEVVFSGDISFDER
                     IEIPNFIAWSPENPHLYGVELRLFSGGTEKDFVRSYFGMRKFSVGRDDKGLPRLMLNN
                     KPYFHNGLLDQGYWPESGLTPPSDEAMICDIKTMKDLGFNMLRKHIKVELERWYYHCD
                     RLGVLVWQDMVSGGGRLSTLYAGVLPNLGIKVDDHNYSIFKREDPQNREEFRRELIEM
                     IDRLYNHTSICLWVPFNEGWGQFEAKEIGEFVKKYDPTRIVDHASGWYDMGGPELISV
                     HRYILPVTLPKITDRPFVLSEFGGYSQVIKGHVWDEKKSFGYKMYKTRAGLTAAYKKL
                     YEKQIIPLIEKGLSAAVYTQVSDVEFEVNGIFTYDRKIIKIEEQTIRELNERMRL"
     gene            9435..10436
                     /locus_tag="PLD98_04120"
     CDS             9435..10436
                     /locus_tag="PLD98_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HQA05469.1"
                     /translation="MAGNEKQKLKLLYLLKIFREKTDDDHVMSAADFCEELLKYGITA
                     ERKSIYKDIEVLKEFGLDIIYTDSPKKGYFLGQREFEEPEIRLLCDAVQAAAFIPNNK
                     KQELLSKIGSLTSDHQFGSIKKQVYIENRSKCENNQVFYNIDLIGKAIEQNKKIKLIY
                     TRRVVSDSKENEYAEREFVVSPYAMIWNDDCYYLVGNYEKYDNLMHMRIDRMKKVAIT
                     DQPARHFSEVSEYKIAFDSADYASKHFNMFSGPTESVSLLCDNELVEAVTDRFGQNTT
                     IIKSGENRFTARFDAAVTPGLVNWIVKFGGKIKVVSPAVLTEMVKERAQQILEVYNA"
     gene            10455..10712
                     /locus_tag="PLD98_04125"
     CDS             10455..10712
                     /locus_tag="PLD98_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HQA05470.1"
                     /translation="MVFRYKTKGTCTSEIVMELDDEHIIKSIEFKNGCSGNLKGIAAL
                     VVGRKAEEVINVLSGITCGFKNTSCPDQLSKALFEAISNEN"
     gene            10764..11429
                     /locus_tag="PLD98_04130"
     CDS             10764..11429
                     /locus_tag="PLD98_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HQA05471.1"
                     /translation="MFADENEFPPFEQIILKNGGEAFEQYGKRFVKLEKGGDTLIGVK
                     SGVGKSNAAAGAAYLISMGADRILNGGLSGAVSHVFRGEIIAGESCAECDFDLRSTGR
                     KLGEKIEGYTALPADEELLNAALKIKGIRKGKLGSGDMFLTDKEKKELYLKTFGINAF
                     DMESGAIACVCASAGVPFLSVRKISDNADDTAVEDYRELDSLQEDDLAKILLIIAGFE
                     SLI"
     gene            11574..>13573
                     /locus_tag="PLD98_04135"
     CDS             11574..>13573
                     /locus_tag="PLD98_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQA05472.1"
                     /translation="MKRFCIKLTAFLACISLILSSAILPAAAFEPDFVFDSGSGSASD
                     PYIIKTRRQFLSFAEAVNSGRGFEGEHINLGADIELNDVGTENWQLYAESFVPIGSCS
                     DTPFKGVFDGKGFSIRGLFCDSSEGGGLFGFINGGEVKNLILLDSFMSSEGAAGGIAA
                     VAVRGKITNCKSFASVSGINSGGIVGIAENTEISSSVNYGGVSGAENAGGIVGSVSLE
                     SKADKCLNLGAVYSPNNAGGIVSDNKGKVTNCKNAGAVSSDTNAGGIAANNLWEISAS
                     ANHGSVSGNVRAGGISAVSSLSISDCYNAGSVWGGEYSAGIAALHGETSSTTNCYNAG
                     EIRGEGQRGAISAVYKLFGFSNCYYLDISCLGGNAQGTPLSHAQMQNRTSFTGFNFLG
                     AWRLNTSGEYKYPTLANTPDAVRVSSVAINKGEAELFLGESFVLNANALPENSTAKYI
                     RFYSENPEIASVTQDGTVNAVNAGETRIFAESADGGFSVFCAVRVLPPKDTIEIIRLN
                     KNELNLQIGDTEQLFCEIVSKRELNLIWTSDDSDIASVNESGLVKAISAGSAVIWVRT
                     EDGTLSDSCLVTVTEPYVPVTGIELNKTYMELEQWEWDYLSATVLPTNANNKAFTWVS
                     SNQSVAVVEPDGRVIGVAPGEADIFAVTDEGGFIASCKVKVKS"
BASE COUNT         3560 a         3072 c         3423 g         3518 t
ORIGIN      
        1 aatcaggtct ttgacctgaa cctttgagtt gtaaaggctg ctgtcggggc tgatttcctc
       61 aatgactatc gttcctgcgg gcagctcttt gagctgaacg actatgctga gattctgata
      121 agagctggcg ggcaggtact ttatgcccag tttcggtctg ttgggaacat agccgttctt
      181 tacaatctgg ttgaatatct cctggaatat cttgctggga accgcaaagc ccataccctc
      241 atagtcggtg gaaacaatct tcattgaatt gatgccgatt acctgaccga gagagtttac
      301 aagggcaccg ccggagttgc cgggattgat tgcggctgta tgctgaatgc atatcattgt
      361 gtagcccgtg ctggaggagt taatcgggcg gtcaatcgcg gagattatgc cgtcggtaaa
      421 ggagccggca aactctgtgc cgccggggtt gccgattgcg taaacttttt gaccaacctc
      481 aagctgttcg gaattgccga attcagcggc tttaagaccc ttagccttga ttctcagaac
      541 gcctatgtca gttctcttgt catagcccac aatctcagcc acatagtcgg tgccgtcata
      601 gagctggaca gatatgctgt tcctgctgct tattacatgg gcgcaggtga gaatgtaggt
      661 atacttgccc tcgctgtcct cgcccattat aacgcccgaa ccttcggtgt agatgctgcc
      721 gtcggaagca tagacaagga taccaacggc ggattcctta atcttcttgt aaacctccgt
      781 ggcggaaagc tctttggctc ccacggggtt atcggtcttg gggatttgag agtcgtttaa
      841 attaagggtg ggcgcgctgt ttgcgccttc aattgtggaa gcctgctcag agggcgcggt
      901 ggtattctcc gaccttacag aggtgaaaat gggataaaaa ccgtagataa cgatagcgag
      961 caccactatg ccgataatga cgccaaagac agttttgccc ttggatttct tgggcggttt
     1021 ggtcgggggc gcgtaatagc ctctgccctg accgtatggg tactgggacg agccctgacc
     1081 ctgaggctga ccctgatagt agggagaacc gaagggatgt ccctgatagc ccgcgggcct
     1141 ttgatagccg ccctgattgg gggcactgct attctgatag ccgccgcttt gattctgagt
     1201 attgttctgc ggctgaggct cggaatttcc tcccgagctg tagataaagc cgctggaggg
     1261 atttatgggg tttgtgttgt tttcggtatt attctcgtca gctctttggc tgttctcgtt
     1321 ttcattgttt ctgagttcat ccgaattcat agataagcct ccaaacaaat ttcttcccaa
     1381 ctgaaaaagg accgtgttaa actatataaa tctaaagaca aagaaatgag tgaaatcgct
     1441 aaaaatttta tagttttttt gtgcaagccc ttttttcgac ttaattaaaa caggggtaaa
     1501 ttaaaagaac attaaagtgc atagtttaat tatagcctaa aggctcagta ttattgcttg
     1561 gaataaagac aagaaattcc gtatattggt ctttaatgct tgtgaccttt atattgccgc
     1621 cgtgcatttc aacaatgctt ttgacgatgt atagaccgag tcccgcgctt ttcgcgtcaa
     1681 ggcttcggga cttgtccacc ttatagaacc tgtcaaaaat gtttccgatt tcctctttcg
     1741 gaatatagct gcccgtattg cgtacggaaa gctcaattcc gtcatttctt tcaaaaatcg
     1801 aaacgctgat tgtgccgccc tcgttggtga acttgaccgc gttgtctata aggttaaata
     1861 ttacctgata aatcatgtcc tcgtcggcgt ttacataaac gctgccgatt ttgtcaagcc
     1921 ccgtgacatt gattctttta tcggaaatgg gcttttcaaa ggtaagcatg gcctttaaga
     1981 cctgctcgcc gatattaaag gttttgggct ttaattgaac ctcgccggtt tcgattttgg
     2041 agagattcag catggaagtt acaagccttg aaaggcggtt aacctcggcg gacacgattc
     2101 tgaggtaatg cttttgattt ttctcgtcaa tggtgccgtc aagtatgccg tctataaagc
     2161 ccgcgattga ggtcatgggg gtttttagct cgtgggagac attagccaca aagctccttc
     2221 tggattcctc aagcgcagcc agtgcgcccg ccatgtagtt tattgaggcg cagagtgtgg
     2281 aaatttcgtc gtcgcctcta acgggcaggc ggtatgcgaa attgccctca gcgtactgct
     2341 tgatggcctg ggacataaga ttcaagggct ttgtaacgct gtctgagatg atgtaaacgc
     2401 tgacaagacc cacgataagg gcgcagaacg cggctataag gaagatataa agcaccgaca
     2461 gaaagtaggg tctgaaagca attccgacag gctgcgcggc gatgacaatg gctttaagtg
     2521 aattgcccga atatacgggc atggctccga cgaaatattt ttctatggag ctggaatatc
     2581 tgcccgcgcc gccaaaaccc tcgggaccta tgctttcgat aagctcccga gttactatta
     2641 tgccgttggt tgaaggctcc gacacaattt ccgtcatgtc atcggagagc atttccttgc
     2701 ttaaaatggg cttgccctcc aagtcgcaga taaaaattgt ggaattggag ctttcggaaa
     2761 gccttgaaag ggcggaacaa ataaggatag cggagttttc gccctcgggg ctgtttaaat
     2821 cgtccagctt gcccgactca tagaagctgc ctacaaagct tgccatgctg atggcgtcgc
     2881 tttgcaggga ctcaatgcac tccctgctcc aaaagccgga tacgaatatc atcatcaaaa
     2941 gccccagaaa aacgaggctt gagagaatta caaacgcaag cacaaaaaag agccttttaa
     3001 agaaggagcc cttttgcatg aaattttgca taagacttat aggcttgagg ccgtttttca
     3061 aaacatttca cctcttagat ggcggataga aaaaaagcgg caacctctgc tcaggattgc
     3121 cgcgtaaagt catttagccg gtgataggcg gttccttggt ctcgaacttg tagcccacgc
     3181 tccagacggt tttaagctcc cactggtcag aaacgccctc cagcttttcg cgcagacgct
     3241 tgacatgaac atcaaccgtt ctggaatcgc cgtagtagtc aaagccccaa acctcatcaa
     3301 gcagctggtc tcttgtaaag accctgttcg gattgcttgc cagatggtag atgagctcca
     3361 gctctttggg cggggtatct atctgaacgc cgtttactct aagctcgtag tttgtaagat
     3421 taatgaccag cttgtcgtag cgtacctctt taatctgctc gggtgtctgg gttgaaacgc
     3481 gccttaatac cgccttgatt cttgccagca cctcttttgt gtcaaagggc ttgacaatat
     3541 agtcgtcggc gcccaactca agacccagaa ccttgtcaaa ggtctcgccc tttgcggtga
     3601 gcattatgac gggcgttgag gatacggagc ggattttgcg gcaaacctgc cagccgtcaa
     3661 gaccgggcag cattatgtca agcagcacaa ggtcaggctt tacggaattg aagctctcca
     3721 ctgcgtcctg gccgttattc agtataacgg ttgtaaagcc ttccttttca aggtacagac
     3781 ggagaagctc gcatatattg acatcatcat cgacaatcat tattttctcg ttagccattt
     3841 ccttactcct ttgactgtga tgtaatctat gtttattgta acataaatct gccgtaatac
     3901 taaaaatatt tacgctatct tcccaattag caaaaagggg tataagctaa ataaacggca
     3961 gtcaaaagac gccgtttata cttttttaga gctgcgaaag aagctattcc tcaaagttta
     4021 gtatctcttc gggaattatt tcaaccttct gctttttctt ggcaagtacc gtcaccacaa
     4081 ccgccgctgc ggcagtaatc ggcagcagta tgtaaaccat attctttttt ataaaggatg
     4141 cggaaccgtc gttttcaaat acgattctgc cgccgtttga aacggcaaga ccgactgcgt
     4201 agccgtttgc gtcggttaca tattcatagc ctttgggcgg tgaggcaaga gattccacag
     4261 gctggtaggg cacaacattg actgccaccg caggctgagt tatctcctcg ggctctttgt
     4321 actcataata acccacgtcg actatatcgg gagcattgtg gagtacgggc gagccgctct
     4381 tggagtcttc cgaatcggga aggaaggaaa gctcaaggag atttgcggcc tcttgagata
     4441 cggtggagaa cagcttgctg tccttgagcg cgccgaagcc tggcagcttt tcggctctga
     4501 gcaagtcaag tccgccgatg ccgccgagta cggagccctt aagcacctgt gagccggcgt
     4561 tgaagaagtc tgagaagaag cctgagattg aatcaaagga tatgtcggag aaggagaagt
     4621 ccggaacgag aagtgacgaa atatccttaa ttctgccggg gagattgccg agcaatgagt
     4681 cgagagcctc ggtaacatcc tcagttttgg ggataagatt ctcgataatg tcgtcaatgg
     4741 tttgattatc ccctttgccg gagcccggtg tgggcgggag ctgtgttccg ttaattattg
     4801 taatattata tgtttttgaa ttggtttgtc cgtccttggt gtaggttact ttaatctgaa
     4861 taacctgctc tttttggttc gggtcgatgg gcacttcgga atagtaatta tccttaatcg
     4921 gcgcgccgtt tgcggtaatg actacctttg tgccctgtcc gtctttataa agagcggcgg
     4981 gattaatcca aacggagctg cgcttgtact tcagctgacc cttgtaatta tatatatcgc
     5041 agtagaactc attttcatca aggtcaaaga aatgggtatt ctgagcgaca gcaatgaatg
     5101 cgtcggcgta gttgctgtct gagcggattg taaggttcgc ggtcttgccc attacctgaa
     5161 ggatataaag aggcactctg tcgttggcga ttgccttctg aagctccgcg gggtcaaggg
     5221 caacgccgaa ctgcctgccg agcatggcgg caaggtattt tatttttatt tcctcgaagc
     5281 tggcgttctt ggcgtctatc gaaatgccct gcttttcgat ggtcatgaca gccatgagcc
     5341 tgtaaacttc ttctttggga gtggatttgc ttacatcgta gcccatggtg tagagggcaa
     5401 tcttgcttgt ggcgattatg tagctttcaa ggttgtccac cgaaacatcg tcaagccact
     5461 gggaaagcag agaaatatcc atgccgaaaa cctttgctat aatctgaatg gcggcggact
     5521 caaggcttgt ccccttcggg aaggaggaaa cgcccaaatt cgacctgctc atcgcggcat
     5581 agagggttct ggcaaggatg agggttgtat tgtctttaag tccgttttcg ggatatttga
     5641 ttccgctgct ctggagccag aactttatgt attcgtcgct gacattaagc tcggtggcgg
     5701 cggaaaattt attgagctgc tccattatgt agaggtaagc cgcttcctgg gagccggggt
     5761 caagctcata gaacttgaga taatagccca caacctgagc aaagctctcg ggagtttcgg
     5821 aatatggata atcgggctgg gcgatgagct ttaccctttc ggaaagaccc tgaaccgtga
     5881 ggacatcttc tttaatgata aggtccttta gccaagcggt gtcgggctgg tcgtatgtaa
     5941 actgggtttc gacaaacata atggctgccg acgcaagcag attgcttgcc agcatggctg
     6001 tgagtattaa aacagaaagg agggtcaatg cgctttttct cgtcgatttc attcgattcc
     6061 tccgcttaaa aggcaaccgt atttgctcca aaacccattt gcagtaaata tataaaaatg
     6121 gtgatatttt taggcatact attctgatac aataataaac atattggtga caaaagtcaa
     6181 tagcaaaaac agtcaagctg actaaagtct tccgaaaatt ggactatttt ttgagctttt
     6241 ttcgcgcttt gctgcgccaa tatttttcat taattgtaaa aaatttacgc gactcagcga
     6301 ccttttcttg cccctcgctt tttcttattt agaaaatcct ttgcgcgtat ttgttttgag
     6361 gttttccccg ttttgaattt ataacgcaga aaaaaacaga ggaaaccaac ttaatggttt
     6421 cctcttattt tttatgggtt aattcttatt tcttcttctt ggtaactacg aatgctgcag
     6481 cggcaacagc gagtgtagct atagcggctg atgcgaatgc gtcaccggaa gaaagaccgg
     6541 tcttcgggat gctgcctgcg ggctttgttg ttgtggttgc gggagccttt gttgttgcag
     6601 gagcctttgt ggtctcttca accttgggct gataggttac agctgcaaga tttacatcag
     6661 cgtcgttgat ctgaacaggc tcaccgtcga ttgtgccggc cttgaaagca gcagccttag
     6721 ccttagcagc gtccttaacc ttaaaggtta cggtgaagat tacgaaattg tcaacattaa
     6781 cgggttcgtc ctcgtcatcg gatgcaacaa ccttgtcagc gtttgcgaca gccgctgtta
     6841 ctgtgcttgt tccgggaaca aggtctgtaa caacattggt tgcggaattc ctggcgtcta
     6901 cgccagctgc tgacttaacg aaggtgtaag cgtcagcgtc aaactcaaga tagaaaccgc
     6961 cggactgaag gcctgctgcg ttcttgagga taacatcaac cttaacctcg cccttgtcat
     7021 tgggctcgcc aaccttgatt gcaagtgccg gagctgcagc aaatgctgaa agagccatgc
     7081 ttgccataag tgcaagtacg actaaaccaa caagtacttt ctttgtaagt ttcatttcaa
     7141 ccatccttta aagatttttt tatataaaga acccgcaccg gttcctttat accttggcac
     7201 agcttatgcc gttattcgag aagtattata tccgattata tccgtagcgt caatacattt
     7261 tagcaaaaat ttctttgccc gtgtaccaaa taacgcataa aaagtcaaat aaaaccggac
     7321 atttttccct tttgattttc aaaaaatatt cctgcagtgc aataccggaa agagtgtttt
     7381 cccccaaatc ccagagagca tcgcccgatt ccgcagattg tcccagcctt gcggttttga
     7441 catttttgca aaacaaggat aaaattacat ataaaggaaa atttctcttt ccgacagtaa
     7501 atttacacta ttttcaaaaa aatttcaagc ccgccgcaaa ataattttca tatttaagac
     7561 ttaaggccgc atagatattg tttcaaattt tgtaacaaat atttttgatt attttttcct
     7621 aaagctgtat aatcataaaa aaaacgaatc ggaagcggag gttgtcagat gtccctcaga
     7681 actcgttttt ctgaaaaaat tgatttgaat tgtcctttaa atgagcatcc aaggccccaa
     7741 ttcatgcgcg agaactggct ctgccttaac ggcaaatacg aatatgccgt cacaaggaac
     7801 ataaacacct tccccaagag ctttgacggg gagattatcg tgcccttcgc gattgagagc
     7861 gagctttcgg gagtcggcag gaagcttttg cccgacgaaa gactttggta caggcgttat
     7921 ttcaccgttc ccgaaaattt taaaggcaaa aggataattt tgcattttga cgccgtcgac
     7981 tggcagtgcc atgtttatgt gaacagacgc cccgccacga ttcacaaggg cggctacagc
     8041 catttcagca ttgacataac cgagcttttg aaagacggcg aaaacgagct tattgtcagg
     8101 gtttacgacc catccgacac gggacttcag caaaggggca agcagagact aaagtcggtg
     8161 ggtatctggt acaccgccac atcgggtatc tggcagccgg tttggcttga ggctgtggag
     8221 gatatttcca tagaaaatat ccgcctcacc cccgattttg acggcggaaa aatcagaata
     8281 gaaagcacag taaaaggaaa agccgaaaag gacaagctcc gcttggaatt gaaagtattc
     8341 gagagcattg acggcggcga ggtcgttttc agcggggata tttcctttga cgagagaata
     8401 gaaatcccga acttcatagc atggagcccc gaaaatccgc atctttacgg cgttgagtta
     8461 aggcttttca gcggcggcac cgaaaaagat tttgtccgca gttatttcgg aatgcgtaaa
     8521 ttctcagtcg gcagggacga caagggtctg ccgaggctaa tgctgaataa caagccctat
     8581 ttccacaacg gtctgcttga ccaaggctac tggccagagt cgggacttac tcccccgtcc
     8641 gacgaggcta tgatttgcga tattaagacg atgaaggatt tgggctttaa tatgctcaga
     8701 aagcatatca aagttgaatt ggagcgctgg tactaccact gcgacaggct gggcgtgctt
     8761 gtctggcagg atatggtcag cggcggcggt cggctgagca ctctttacgc gggcgttctg
     8821 ccgaacctcg gaattaaggt tgacgaccac aattactcaa tctttaaaag agaggacccg
     8881 caaaaccgcg aagaattcag acgcgagctt atagaaatga ttgaccgcct gtataatcat
     8941 acctccatct gcctgtgggt tccctttaac gagggctggg gtcagtttga ggcaaaggaa
     9001 atcggcgaat ttgttaaaaa gtacgacccg acaaggatag tcgaccacgc aagcggctgg
     9061 tacgacatgg gcggtcccga gcttataagc gttcaccgct atattcttcc cgtaacgctc
     9121 ccgaaaatca ccgacagacc ctttgtttta agcgagttcg gcggctattc tcaggtaatt
     9181 aaaggccatg tctgggatga gaaaaagagt ttcggctaca agatgtataa aacccgcgca
     9241 gggcttaccg cagcttacaa aaagctctat gaaaagcaga ttatccccct tatagaaaag
     9301 ggtctttccg cagcggttta cacacaggtt tccgatgttg agttcgaggt caacggcatt
     9361 ttcacttatg acaggaaaat cataaagatt gaagagcaga caataagaga gcttaacgaa
     9421 aggatgcgtc tgtaatggca ggcaacgaaa agcagaaact taagctcctg tatcttttaa
     9481 agattttcag agaaaagacg gacgacgacc atgtgatgtc cgccgccgat ttttgcgagg
     9541 agctgttaaa gtacggcatc acagcggagc gcaagtccat ctacaaggat atagaggttt
     9601 taaaggagtt cgggcttgac atcatttata ccgactcccc gaaaaagggc tactttttgg
     9661 ggcagcggga atttgaggag cctgaaataa ggcttttgtg cgacgccgtt caggccgcag
     9721 cctttattcc gaacaacaaa aagcaggagc tgctttctaa aatcggctct ctcaccagcg
     9781 accatcagtt cggcagcata aaaaagcagg tatatatcga aaacaggtca aaatgcgaaa
     9841 acaaccaggt cttttacaat atcgacctta taggcaaggc gattgagcag aacaaaaaga
     9901 taaaacttat atacacaagg cgggttgttt cggattccaa ggagaacgag tacgcggaaa
     9961 gggagtttgt cgtttctccc tacgcaatga tttggaacga cgactgctat taccttgtgg
    10021 gcaattacga aaaatacgac aatctcatgc acatgagaat agaccgcatg aaaaaggttg
    10081 ctataaccga ccagccggca aggcatttct ccgaggtcag cgagtacaaa atcgcctttg
    10141 actccgccga ctatgcctca aagcatttca atatgttttc gggtcccacc gagagcgtca
    10201 gcctgctttg cgacaacgag cttgtggagg ctgtgaccga ccgcttcggt cagaacacga
    10261 ccataataaa gtccggcgaa aaccgcttta ccgcaaggtt tgacgcggcg gtcactccgg
    10321 gacttgtcaa ctggatagtg aaattcggcg gcaagataaa ggtcgtctcc cccgctgttc
    10381 ttacggaaat ggtaaaagag agggctcagc agattttaga ggtttataac gcataacaag
    10441 gaaggtcaac agcaatggtt ttcaggtaca aaactaaagg cacatgcaca agcgaaatcg
    10501 ttatggagct tgacgacgaa catataataa aatcaattga atttaaaaac ggctgcagcg
    10561 gcaatttaaa gggcatagcc gcccttgtgg tgggcagaaa ggcggaggag gttataaatg
    10621 tgctgtcagg cataacctgc ggctttaaaa acacctcctg ccccgaccag ctctcaaagg
    10681 ctttgtttga ggcaatcagc aacgagaatt agccaaagcg gcttacttta gaaaaaggac
    10741 tgataaacat tacaacgggt tttatttttg cggacgagaa cgagtttcct ccttttgagc
    10801 aaatcatact gaaaaacggc ggagaagcct ttgagcaata cggcaagcgc tttgtaaagc
    10861 tcgaaaaggg cggcgacact ttgataggcg taaaaagcgg cgttggcaag tcgaacgccg
    10921 ccgcgggcgc ggcttaccta atatccatgg gagccgacag aattttaaac ggcgggcttt
    10981 cgggcgccgt cagccatgtg ttcagaggcg agattatcgc cggagaaagc tgcgcggagt
    11041 gcgattttga cctcagatca acgggcagaa agctcggcga aaaaatcgaa ggatataccg
    11101 ccctccccgc cgacgaggag cttttaaacg ctgctttgaa gattaaaggc attcggaaag
    11161 gaaaactggg cagcggggat atgttcctta ccgacaagga gaaaaaagag ctttacctta
    11221 aaacattcgg catcaacgcc tttgatatgg aaagcggagc gattgcctgt gtatgcgcct
    11281 cagcgggagt gcctttcctc agcgtaagga aaatctccga caatgccgac gataccgccg
    11341 ttgaggatta cagagagctg gacagccttc aagaggacga tttagccaaa atccttctca
    11401 ttatagcggg ctttgagagc ctgatataaa ccgaaaagca atgagccgcc gagggcggct
    11461 ttttttatgc ccatttaccg aacacttgtt tcgttgacgg tttttgtaaa aaattgtata
    11521 attagataaa attttgctat attttcccga aaagaattat tgggggtaaa gaaatgaaga
    11581 gattttgcat aaagctcacg gcttttttgg catgcatctc cttaatatta agctccgcaa
    11641 tcctgcccgc cgccgcgttt gagccggact ttgtcttcga ctcgggcagc ggctcagcct
    11701 ctgaccctta tataataaaa acccgcaggc agtttttaag ctttgccgag gctgtaaact
    11761 cgggccgcgg ctttgagggt gagcatataa atctcggggc ggatattgaa ctgaatgatg
    11821 tgggaactga aaactggcag ctttatgcgg aatcttttgt tccgataggc tcttgctccg
    11881 acactccttt taaaggcgtt tttgacggca agggttttag tatacggggg ctgttctgcg
    11941 acagttccga gggcggcggg cttttcggct ttataaacgg cggggaagtg aaaaatctta
    12001 ttctccttga ctcctttatg tcctccgagg gcgccgcggg gggtattgcc gcagtcgctg
    12061 ttcgcggaaa aatcacaaac tgcaagagct ttgcgagcgt cagcggcata aactcgggcg
    12121 gcatcgtggg cattgccgaa aacactgaga tttcttcctc ggtaaactac ggcggtgtca
    12181 gcggcgctga gaacgcgggg ggcatcgtcg gcagcgtttc tttggagtcg aaagcggaca
    12241 agtgcctgaa tctcggcgcg gtttactccc caaacaacgc gggcggcatt gtctcggaca
    12301 ataagggcaa agttacaaac tgcaaaaacg caggcgcggt ttcttctgac actaacgcgg
    12361 gaggtatagc cgcgaacaat ttgtgggaga tttccgcttc tgccaatcac ggcagcgtga
    12421 gcggaaatgt cagagccggc ggcattagcg cggtgagcag cctttcaata agcgactgct
    12481 acaacgcagg gtcagtgtgg ggcggtgagt attccgccgg catagccgct ttgcacgggg
    12541 aaacaagctc cacaaccaac tgctacaatg cgggagaaat caggggcgaa gggcaaaggg
    12601 gagcgatttc cgccgtatac aaactctttg gtttcagcaa ttgctactat cttgacataa
    12661 gctgcttggg cgggaacgct cagggaactc ctttaagcca cgctcaaatg cagaacagaa
    12721 ccagttttac aggctttaac tttttaggag cttggaggct caatacctcg ggagaatata
    12781 aatatcccac cctcgcgaat acccccgacg ctgtaagggt cagctccgtt gcaataaata
    12841 aaggcgaagc ggaactgttt ttgggcgaga gttttgtttt aaacgccaat gcgctccccg
    12901 aaaacagcac cgcaaaatat atcaggtttt actctgaaaa tccagaaatt gcgtcagtca
    12961 cgcaagacgg gacggtgaac gccgtaaatg cgggcgaaac gaggattttt gccgagagcg
    13021 ctgacggcgg ctttagcgtt ttttgcgccg ttcgggtttt gccgccgaaa gacactatcg
    13081 aaataataag gctcaacaaa aatgagctga atctccaaat aggagatacc gagcagcttt
    13141 tctgcgagat tgtctccaaa agagaactaa atctcatttg gacaagcgat gactccgata
    13201 ttgcgtcggt caacgaaagc gggcttgtta aagccatatc tgcgggcagc gctgtaatat
    13261 gggtcagaac ggaggacggg acgctcagcg acagctgtct tgttaccgta accgagccat
    13321 atgtgccggt tacgggcatt gagcttaaca aaacatatat ggagctggag cagtgggaat
    13381 gggattatct ctccgcaact gttctcccga caaacgccaa taacaaggcg tttacatggg
    13441 tcagcagcaa tcaaagcgtc gccgttgtgg agcctgacgg aagggttatt ggcgtagcgc
    13501 ccggagaggc ggacattttc gccgtcaccg atgagggcgg ctttatagca agctgcaagg
    13561 taaaggtaaa gtc
//
LOCUS       DAONVQ010000043        13377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3538_length_13377_cov_4.501726, whole genome shotgun sequence.
ACCESSION   DAONVQ010000043 DAONVQ010000000
VERSION     DAONVQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13377
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3538_length_13377_cov_4.501726"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..364)
                     /locus_tag="PLD98_04140"
     CDS             complement(119..364)
                     /locus_tag="PLD98_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05473.1"
                     /translation="MFNFALVTTLEIAAVLLIILGFIYEHKVIEFEDKMFDKITMRLR
                     QNRRYKAELKTAEFLERSRYVPEIPEDDEEEVRGYVA"
     gene            complement(652..1314)
                     /locus_tag="PLD98_04145"
     CDS             complement(652..1314)
                     /locus_tag="PLD98_04145"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQA05474.1"
                     /translation="MQFKAVVFDLDGTLIDTIEDLKDSLNFVLEKRGCPKRTYDEVKR
                     FVGNGLGKLMERGLPQGAGPELIKECTAELISYYSEHSLIKTAPYEGIKELISDLKTR
                     GMKLSVVTNKNEKEAKRICAHFFGDVFDTIVGDREGMPLKPAAEPVFKALEEMGVSAD
                     DAIYIGDSEVDVRTALNSGLRMIAVLWGFREKDVLIKEGAKVFAEKPSDILKVEAQKV
                     CP"
     gene            complement(1316..3166)
                     /gene="uvrC"
                     /locus_tag="PLD98_04150"
     CDS             complement(1316..3166)
                     /gene="uvrC"
                     /locus_tag="PLD98_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQA05475.1"
                     /translation="MDERLKTLHDKALSLPHQPGVYLMKDSSGKVIYVGKAKSLKNRV
                     TTYFGSQDKHTTKVRKMVSNVKDFDYIVVQSEFEALLLECSLIKQYMPKYNILLKDDK
                     GYSYIKVTPPPWSRISAVKQKDDDNARYLGPYTGSGYVTNAVEQALKIYKLPTCSKEF
                     PRDIGKSRPCLNYFINNCSAPCAGKISQAEYEEAVNSAIKFIQQGGAATKKELIAQMN
                     EAAENLEFERAAKLRDRIAAIDRVGRSQRVVAQKVKNQDVIAASTAGEITCFAVLRFR
                     DGNIVDTEHFFIETDEDMEYARAHFLLSYYQIRTDIPPIVTLDGEVEDIWLIKEWLKE
                     KRGSLVRINIPVRGEQKELVEICRKNALEKLSQRIGRQSSEVSVLEELMQLIGLKKLP
                     EYIEAYDISHTAGKNSVGAMVVFKNAKPYKSAYRRFVIKSFEGNDDYRSIAEVLTRRA
                     GEYERCKGTENTFGVKPDIILIDGGIGQVHAAREALKNTPLSDVPVFGMVKDSRHRTR
                     AMTDDGDEITIDSKRRVFSLISEIQNEVHRFAIEYHRKKSSSASKSLTLMEIEGVGKQ
                     RAKELIKAFKTIDKISKASVEELMSVKGISKAVAENIYNHFHKEKQDTEN"
     gene            complement(3241..3510)
                     /locus_tag="PLD98_04155"
     CDS             complement(3241..3510)
                     /locus_tag="PLD98_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HQA05476.1"
                     /translation="MYVKDVVVQNQVGLHARPATFFIQKANEFKSSIWVEKDERRANA
                     KSLLGVLSLGIMGGTSIKIIAGGPDEEKAVNELAKLVESGFAETE"
     gene            complement(3707..4669)
                     /locus_tag="PLD98_04160"
     CDS             complement(3707..4669)
                     /locus_tag="PLD98_04160"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQA05477.1"
                     /translation="MEKMKKKTSISILLTFCITLAVVACVFIYKQVNNLLLEKKIDTP
                     QAPIQTEAAGGTISMPSEEPTTEEKTEAPTETTTKAPAKEPTTKKQTPSNNTGGGSLE
                     AKIKKLNGKGKKIYLTFDDGPSKLTPQILDILDKYNVKATFFVIGNGTNHSYLSKIAE
                     KGHAIGLHTYSHNYKYIYASEENFFDDLEKISKLVEQETGVVSKIIRFPGGSSNLSSK
                     FNPGVMSRLAEKVKAKGYEYYDWNCTNGDGTGNNLSVSTLINTAVDTALKANGDLIML
                     MHDSAAKKTTVEALPRIIEIFRDAGYTFAPISPETVPVHHTIKN"
     gene            complement(4804..5715)
                     /locus_tag="PLD98_04165"
     CDS             complement(4804..5715)
                     /locus_tag="PLD98_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQA05478.1"
                     /translation="MEIAAEILTRFWQITEKKDKARLSKQRPADGIDGRFDIPYINDG
                     ETEHLLDVYFPENTKENLPAIIDIHGGGWMYGNKELNKNYCYYLASRGFTIVNINYTL
                     APKATVDGQIRDVFAALKWVDENADEYFIDKNNLFLTGDSAGGHLAALTTVIMSDRPD
                     LELLYNVECPSFRFKAIGLTCGAFNLDIILNNRIFVAKKYAEFLLGSDFENSPYKNSV
                     SMRDVLCGTKIPPVYLASSAEDFIKRQSDAFDALLTKYNLPHFYRRMGRVKGKHLSHV
                     FNILYPDWEESIFVNNEMTDFFKSYID"
     gene            complement(5781..7016)
                     /locus_tag="PLD98_04170"
     CDS             complement(5781..7016)
                     /locus_tag="PLD98_04170"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQA05479.1"
                     /translation="MKTIKVAAIISEYNPFHNGHKYHVERTRAMGATHVVAVMSGNFV
                     QRCEPALIDKRHRAKMALMSGIDLVIELPLPWATASAERFAKGAVQIINAMPAVEILS
                     FGSESGDVERLKNAAEILLDETVEEEILNALDSGISYPAARERAIAKIAGAKVASVLK
                     TPNDILGIEYIKALITTDSKLTPMPIERRGAAHDGKSYEKGFMSASEIRRAILSAENS
                     DRDPKDILTEKLSEYLPEASIEILSDGIKKGEAPCSFSQFETAVLYALRRLSAEDYLS
                     FPDVSEGLHNRIYAAVNSSASLSEIYSRAKTKRYTLSRIRRIVLSAFLGIERDIALNE
                     PPYIRVLGLNERGREILREIRKTAAKPIIMRAADIKKCDDFSKRVFSLEAASTDIYAL
                     AKPVKGACGSEYTDNVVLL"
     gene            complement(7107..8747)
                     /locus_tag="PLD98_04175"
     CDS             complement(7107..8747)
                     /locus_tag="PLD98_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA05480.1"
                     /translation="MSKSVELNTYTKKERNAYLAGLAGQNIMYNIISQTFSYYLQFVL
                     AIPAGIVGGILAVLKVWDGINDPVMGHIIDRTRTKWGKCRPYLIFLPVPIFFVTMFCF
                     LNGFYDTSLPSTALRNIAVVAWAALAYFLWDILYTAGDVPLWGITALMTESDKDRIKL
                     LSLARIFAGVGSAVALLSIQSVSFALRDALVPFFDTPIKAERAGFILSALIFAVVGTA
                     LFQLTGLMTRERITPSPEKNSLLDSFKVMYKNKPFRQLMISGILASPKQLVTIAAFPL
                     VSYYYANKDGALNTLYIAILGGSVMIGAFVATALTPKILQKVSKKDLYNYSNLFGAIP
                     FAAIMLLYMAAPYDMAKPFYIAVCFVLFFFAGASNGFTAVLTSLMIADAVDYEEYRTG
                     IRTDGVFFAGQSFITKLTTGLATIVSGIAYAVVGFSDANVEKINAYISAGGIARENPE
                     FQPYMFIMFFLVSVPPLIGSLLMVIPTWKYALSDERHKQILAELNEKRHAARMAAEAQ
                     ESVAELAAEAKESDAEIIETLTEEPQENTEAVTQENNE"
     gene            complement(8849..10228)
                     /locus_tag="PLD98_04180"
     CDS             complement(8849..10228)
                     /locus_tag="PLD98_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HQA05481.1"
                     /translation="MLNRFKSAISVALVAVLLFTAFPIATFAEDTYNIKFSVTKLNLS
                     RDELESFLGDLNEDLPTEADGVYPIPLIIRVGVSGSGKFVGGSFEVEYNGKKLKFGGA
                     VSNSDSILDDDGNLAVSNEISIANSDNKRVHVAFFTTDYYQLSSSGGSITYLLFYVKR
                     NPDSFDGKFHYYEAMNFEIRPEYVGFQKSDGGVEVLRPATFGSRVTASVQSNIIPDTP
                     APIDSLKFEVSIPDKYTVDPTKKNYTFSNELKSGNIRAYRRDGTSELLLRYESGRQPN
                     RFKVEPVDLSTPGMKTLRVICIDGVTNREIVLRNNIEVVAASGVTSISVTPPTNLYNT
                     VGNALDTKGMRVRAKMSGSSGEVEVTSGYTITGYNKDKVGEQTITVTYNGKSASFKVY
                     VCPIKGDADNNGKVQPADARVVLRVYAKLQSTNKAFEHSMDVDGNGKVNASDARKILR
                     YCAKLDKTL"
     gene            10378..11649
                     /locus_tag="PLD98_04185"
     CDS             10378..11649
                     /locus_tag="PLD98_04185"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:NF009814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HQA05482.1"
                     /translation="MKILLSDEVKLVIDRFSEHGYTAYAVGGCVRDLLLSLNPKDWDV
                     ASPASPDETKEIFKDFKVLDTGIKHGTVTVIINSMPIEVTAFRTEGAYSDRRHPDFVE
                     FTKDIESDLKRRDFTVNALAYNPIRGIVDISGGLDDLKNKVIRTVGNPKERFDEDPLR
                     ILRALRFASALGFTIEEETEKAVHKYHPLLGEIAAERIYAELKALFSGQRIEYVLSGF
                     KDVVAFLFPEIKESLYSGYEALFGEVSLKSEEVFKAISLLRGDFYLRAALLFSSGKEA
                     DAEMILNRLRSDKATSGKVQKLVKALKLPLPSTKADMKRFINAFGFDAAEDYLIAAEK
                     VGCAIESKALPNLSSAKSFFETIRKNKEAVTIHDLDIKGTDIQNLGFKGAEVGKALEI
                     LLDAVFEEECENTKVDLIRFIKNRSEDIKHK"
     gene            11665..>13377
                     /locus_tag="PLD98_04190"
     CDS             11665..>13377
                     /locus_tag="PLD98_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQA05483.1"
                     /translation="MRKVINLNFGWKYSPVFEDEMLKKSFDDSSFETVDIPHANKELP
                     LNYFDEREYMFVSCYRKKFKLDKGDYADGKRVLLHFEGVACRSLVYVNGKYAGEYKGA
                     YTPFTFDITDHLSSRDNVIAVRVDSTETPDMPPFGKVVDYLCYGGIYREVWLECVEKE
                     HIEDIFVRTGNVLDKKKLLTADITFSDKLKGELKLELLDGEKVIAERTTEFDAKVFRV
                     KWGIQGVQNWDIENPKLYTLRVTFGKDVKEVRFGFREARFTKNGFYLNGKRIKLIGLN
                     RHQSYPYVGYAMPKSAQEADADLLKFTLGCNIVRTSHYPVSKHFLNRCDEIGLLVFTE
                     MPGWQYTDMNPGEWQDNAIENVRRMIIRDRNHPSIVLWGVRINEGADCDALYEKTNAL
                     ARSLDPTRQTGGVRNFPQSHLLEDVYTYNDFSHSGGKIKLLPPTVVAGPNAPYLVTEF
                     NGHMFPTKSYDREDIRTEHALRHARVQSASFGNDRISGAIGWCMSDYNTHCDFGSGDR
                     ICYHGVTDIFRIPKLAAAVYASQQDYIPVLEVSSSMDIGDHPKSFRGQIYIFTNCDYV
                     KVYKN"
BASE COUNT         3345 a         3057 c         3168 g         3807 t
ORIGIN      
        1 acagcggaca ggcacaataa aaaacagggg cgctggaaac ggcccctgtt tatgtttatg
       61 ggatacaaaa aagggagggc gcagaacgcg ccctcattat tatgtataac ggagaagatt
      121 atgccacata acctcgcact tcttcttcat cgtcctcggg aatctcggga acatacctgc
      181 ttctttctaa aaattcggca gttttaagct ctgccttata ccttctgttt tgccttaatc
      241 tcatggtgat tttatcgaac attttatcct caaactcaat aaccttatgt tcatatataa
      301 aaccaaggat gattaaaagc actgctgcta tttcaagggt tgtaaccaaa gcgaagttaa
      361 acatattatt acctccattg gagaacattt gttttgttgg ttttagtata cagatttttc
      421 atcgacctgt caatacaaat gttcggttat ttcgacattt aatgtcccat aaatgggact
      481 attttgacga tttatagaga atcttaaatg gaaaaaatat actgctatga gaataatagc
      541 acaatatatt gtgcttgtca agagtgttat atactaagtg atgtgtcgaa taatggagaa
      601 ataaaaaacg ggcgttaaag cccgtttaat tttataagat attctcacga tttacggaca
      661 gaccttttgc gcctcgactt taagaatgtc cgagggtttt tcggcaaata cctttgcgcc
      721 ttcttttatg agaacatcct tttccctaaa gccccaaagc accgcaatca ttctgagccc
      781 cgagtttaag gctgttctta catccacctc ggaatcgcct atataaattg cgtcgtcggc
      841 gctgactccc atttcctcaa gagccttgaa taccggttcg gcggcgggct tcagcggcat
      901 accctccctg tcgccaacaa tggtgtcaaa gacatcaccg aaaaagtgag cgcatattct
      961 ttttgcctct ttttcgttct tgtttgtaac gacgctaagt ttcatgcccc ttgtctttaa
     1021 atcgcttata agctccttta tgccctcata aggggctgtt tttataaggg agtgctccga
     1081 atagtagcta atcagctccg cggtgcattc ttttataagc tcggggccgg caccttgggg
     1141 aagtcccctt tccatcagct ttccgagtcc gttgcccaca aagcgtttaa cctcatcgta
     1201 ggttcttttg gggcagcctc tcttttcgag gacaaaattc aaactgtcct ttaaatcctc
     1261 aattgtgtca ataagtgtac cgtccaaatc aaaaactact gctttaaact gcatcttaat
     1321 tctctgtatc ctgtttttct ttatggaagt gattatatat attttccgca actgctttgc
     1381 ttatgccttt aacgctcata agctcttcta cgcttgcctt tgagattttg tcaatagtct
     1441 taaaggcttt tataagctct tttgccctct gcttgcctac tccctcaatt tccattaaag
     1501 tgaggctctt tgaggctgag gagctttttt tgcggtgata ttcaatggcg aatctgtgaa
     1561 cctcgttttg aatctcggaa ataaggctga acactctgcg ctttgagtcg atagtgattt
     1621 cgtctccgtc gtcggtcatt gctcttgtgc ggtggcggct gtccttaacc atgccgaaaa
     1681 cgggcacatc ggataagggg gtgtttttga gagcctctct tgccgcgtgt acctgaccaa
     1741 tgccgccgtc gataagaata atgtcgggct taacgccgaa ggtattttcg gtgcccttgc
     1801 atctttcgta ctcccctgct ctgcgggtca gaacctcggc tattgagcgg tagtcatcgt
     1861 taccctcaaa ggactttatt acaaagcggc ggtacgcgct cttatagggc tttgcgtttt
     1921 taaagaccac catcgcgccc acactgttct tgccagcggt gtgggagatg tcgtacgcct
     1981 caatgtattc gggcagcttt ttaagaccta tgagctgcat gagctcctca agaacgctta
     2041 cctcgctgct ttgtctgccg attctctggg acagcttttc aagggcgttc tttctgcaga
     2101 tttccacaag ctccttctgc tcgcccctga cggggatatt gattctgaca agtgagcccc
     2161 gcttttcttt aagccactcc tttatgagcc aaatatcctc aacctcgccg tcaagggtta
     2221 caatgggcgg aatgtcggtt ctgatttggt agtagctgag aaggaaatgg gctctggcgt
     2281 attccatatc ctcgtcggtt tcaataaaga aatgctcggt gtccacaata ttgccgtccc
     2341 tgaatcgcag gaccgcaaag caggttatct cccccgcggt cgacgcggct atgacatcct
     2401 gattcttgac cttttgagcc acgacccttt gggagcggcc cactctgtct atggcggcta
     2461 ttctgtcccg cagctttgcc gccctttcaa actcaaggtt ttccgccgcc tcgttcatct
     2521 gggcgataag ctccttttta gtagcggctc caccctgctg aatgaattta atcgcgctgt
     2581 ttacagcctc ctcgtattca gcttgtgaaa tttttcccgc gcagggagcg ctgcagttgt
     2641 ttataaagta gttaagacaa gggcgggatt ttcctatatc cctcgggaac tcctttgagc
     2701 aggtggggag cttgtatatt tttaaagcct gctcaacggc gtttgtcaca tagcccgaac
     2761 ccgtataggg tcctaaatag cgggcattgt cgtcgtcctt ttgcttgacc gccgatattc
     2821 tcgaccacgg cggcggggtg acttttatat agctgtagcc cttgtcatcc tttaaaagga
     2881 tattgtactt gggcatgtac tgctttataa gcgagcactc aagcagcagc gcctcaaact
     2941 cgctttgaac aacgatatag tcaaaatcct tgacatttga gaccattttt ctgaccttgg
     3001 ttgtatgctt gtcctgagag ccgaaatagg ttgtgacacg gtttttaagg ctcttggctt
     3061 ttcccacata gatgaccttg ccgctggagt ctttcattaa atagactccc ggctggtggg
     3121 gcaggctcaa tgccttatcg tgaagcgttt tcaatctttc gtccatgctt cctccgtcag
     3181 aaatcggttt ataataaaaa aggcatcgga gtattctccg acgccgcatc aagtccttag
     3241 ttattctgtt tcggcaaagc cggattcaac cagcttagca agctcattaa ctgctttttc
     3301 ctcatccggg ccgccggcaa ttatctttat tgatgtgccg cccatgattc cgagtgagag
     3361 aactcctaaa aggctctttg cgtttgctct gcgctcatcc ttttcaaccc aaatggatga
     3421 cttaaattcg tttgccttct ggataaaaaa tgtagccgga cgggcatgta gacccacctg
     3481 attttgcact acaacatctt taacatacat atacgaaagt ctcccaacca attgtattct
     3541 ttcacactct taaatattat atcacaaaaa accgaatcgt caacggttgg gcagataaat
     3601 aagcctttag gagcgatttt tcataggaat gctaaactat atgaattcgg aatttaacta
     3661 tttgtgcaaa atgacaatgt aattttatca aattttacaa attaattcag tttttgatag
     3721 tgtgatggac gggcactgtt tcgggagaaa ttggggcaaa agtatagccc gcgtccctga
     3781 aaatctcgat aattcgaggc agtgcctcta ccgtcgtctt tttagcggcg gagtcgtgca
     3841 tcagcataat aaggtctccg tttgccttca aagctgtgtc cacagcggtg tttataagtg
     3901 tgcttacgct gaggttattg cctgtgccgt cgccgttggt gcagttccag tcgtaatact
     3961 cgtagccctt ggctttgacc ttttcggcaa ggcgggacat tacgccgggg ttaaacttgc
     4021 tggagaggtt gctgctgccg ccggggaatc tgattatctt tgataccaca cctgtttcct
     4081 gctctacgag ctttgaaatc ttttcaaggt cgtcaaagaa attctcctcg gaggcgtaga
     4141 tgtatttata attgtgggaa tatgtatgca gaccgattgc atgacccttc tcggcgattt
     4201 ttgaaagata gctgtggttt gtgccgttgc ctattacaaa gaaggttgcc ttaacattat
     4261 acttatcgag aatatcgaga atttggggtg tgagctttga gggaccgtcg tcaaaggtaa
     4321 ggtagatttt cttgcccttg ccgttgagct tctttatctt tgcttcaaga cttccgccgc
     4381 cggtattatt tgagggagtc tgctttttgg tggtcggctc tttggcggga gccttggttg
     4441 ttgtttctgt gggtgcctca gttttttctt cggttgtagg ctcttcagaa ggcatggaaa
     4501 tggtgccccc tgctgcctct gtctgaatgg gtgcctgggg agtgtctatc ttcttctcca
     4561 aaagcagatt gtttacctgc ttgtagataa acacgcaagc gactaccgct aaggtgatgc
     4621 agaaggttag taaaatgctg atagaggttt ttttcttcat cttttccatt taaagtacct
     4681 gccctattga tttcacaata aagtttacag catatattct ttcaaatcaa tgaaaatagg
     4741 gtaacaaaga tttacaatat tctatctttt ttgaaagaat attttggcag gattaaagcg
     4801 gctttaatca atataagact taaagaaatc ggtcatctcg ttgtttacaa agatgctttc
     4861 ctcccaatcg ggataaagga tattgaaaac atggcttaag tgcttgccct ttaccctgcc
     4921 cattctgcga tagaagtggg gcagattgta ttttgtaagc agggcgtcaa aggcgtctga
     4981 ctggcgtttt ataaaatcct ccgcgctgct ggcaagatat acgggcggga ttttggtgcc
     5041 gcaaagcaca tcacgcattg agaccgaatt cttgtagggt gaattctcga aatcgctgcc
     5101 caaaaggaac tcggcatact ttttagccac gaaaatccta ttgttaagga taatgtccag
     5161 attgaacgcg ccgcaggtaa gaccaatggc tttgaacctg aatgaggggc attccacatt
     5221 gtataaaagc tccaaatcgg gtctgtcgga cataataacg gttgtgagag ccgcaagatg
     5281 tccgcccgcg gaatcgcccg tcaggaacaa attattcttg tcaataaaat actcgtcggc
     5341 gttttcgtcc acccatttta aagcggcgaa aacatcccta atctgaccat ccacggtcgc
     5401 ttttggagca agggtgtagt tgatgttgac gatggtgaaa cctcttgagg caagatagta
     5461 gcagtagttt ttgttaagct ccttgttgcc gtacatccag ccgccgccgt gaatgtctat
     5521 gattgcgggc agattctcct ttgtgttttc tgggaaatac acatccaaaa gatgctccgt
     5581 ctccccgtcg tttatatacg ggatgtcaaa tctgccgtca atgccgtcgg ccggtctttg
     5641 cttggacagt ctcgctttgt ctttcttttc ggttatttgc cagaatcttg tgagaatttc
     5701 agcggcgatt tccatttcac acctccaaaa agctagcctt tttatacata ctctgaatat
     5761 aaagccttta tgaaaaacgg ctacaagaga accacattgt ccgtgtattc cgagccgcaa
     5821 gcgcctttta cgggctttgc gagggcgtaa atatcggtgg aggcagcctc cagagagaat
     5881 accctcttgc tgaaatcatc gcacttttta atgtccgccg cgcgcattat aatgggcttt
     5941 gcggcggttt ttcttatctc cctcaatatc tccctgcctc tttcattaag ccccaaaact
     6001 ctgatatacg gcggctcgtt aagggctatg tccctttcta tgccgaggaa tgcggagagc
     6061 acgattcttc tgattcttga gagtgtgtat ctttttgtct ttgcccggga gtatatttcg
     6121 ctaaggctcg ctgagctgtt tacagcggcg taaattctgt tgtgaagccc ctcggataca
     6181 tcggggaaag aaaggtaatc ctctgccgac agccttctta aagcgtacaa aacggcggtt
     6241 tcaaactggg aaaatgagca gggcgcctca cccttcttta ttccatccga aagtatttca
     6301 atgctggctt cgggcagata ttccgagagc ttttcggtca gtatatcctt tgggtctcta
     6361 tcggagtttt ccgcgctcag tatcgctctt cttatctccg aggcggacat aaagcccttt
     6421 tcgtaagatt ttccgtcatg ggcggctccc cttctttcga ttggcatggg ggtgagcttt
     6481 gagtctgttg tgataagagc cttaatatat tcaatgccga gtatgtcgtt tggggttttt
     6541 aagacggagg cgaccttggc tcccgcgatt ttggcgatag ccctttccct tgcggcggga
     6601 tagctgatac cgctgtcaag agcgtttaaa atctcctcct ccactgtttc gtcaaggagg
     6661 atttcagcgg cgtttttaag cctttcaaca tctccgcttt cgctgccgaa tgacaggatt
     6721 tccacagcgg gcatggcgtt tattatttgc actgcgccct ttgcaaaacg ctcagcggag
     6781 gcggttgccc aagggagcgg cagttctata acaaggtcaa tgcccgacat cagagccatt
     6841 ttggctctgt gccgcttgtc gattaaagcg ggctcgcagc gctgaacgaa attgccgctc
     6901 ataaccgcaa caacatgggt cgcgcccatt gcccttgttc tctcaacatg atatttgtga
     6961 ccgttatgga agggattgta ctcgcttatt attgccgcaa ctttaatggt tttcacttcc
     7021 tttggcagtt tactatattt taacacaccg aaagggctta ttcaaatatc aaacttaaag
     7081 ggcaggattt ttcctgccct taaactttat tcgttatttt cttgtgttac cgcttcggta
     7141 ttctcctgag gctcttcagt cagagtctcg attatttcgg catcgctttc cttagcttcg
     7201 gcagccagtt cggcaacgga ctcctgagcc tcggcagcca ttcttgccgc atgtctcttt
     7261 tcgttaagct cggcgagaat ctgcttgtgc ctctcgtcgc tgagagcgta tttccatgtc
     7321 gggatgacca tgagaagtga gccgataagc ggaggaacgg atacaaggaa gaacattata
     7381 aacatataag gctggaactc ggggttctct cttgcgattc cgccagcgct tatataagcg
     7441 ttaatctttt ctacattggc gtcggagaag cctaccacag cataggcaat gccgctgact
     7501 atggttgcaa gaccggttgt gagctttgtg atgaatgact gtccggcgaa gaatactccg
     7561 tcggttctga tgcccgtgcg gtattcctca tagtcgacgg cgtcggcaat catcagggat
     7621 gtaagcactg cggtaaagcc gtttgaagcg cctgcgaaga agaagagcac aaagcagacg
     7681 gcaatataga agggctttgc catatcatag ggagcggcca tgtaaagaag catgattgcg
     7741 gcgaagggaa ttgctccgaa aagattgctg taattgtaaa ggtctttttt gctgactttc
     7801 tgcagtattt tgggagtgag ggcggtagcg acaaaggcgc ctatcattac ggacccgccc
     7861 agaatggcta tgtataaggt gtttaaagcg ccgtccttgt ttgcgtagta gtatgaaaca
     7921 agcgggaacg ctgcgattgt gacaagctgc ttgggacttg cgagtatgcc cgaaatcata
     7981 agctgacgga aaggcttgtt tttatacata accttaaagc tgtcaaggag actgttcttt
     8041 tcgggcgagg gagttattct ctcccttgtc ataagacccg taagctggaa caatgctgtg
     8101 ccgactacgg caaagattaa ggcgctgagt ataaagcccg ctctctctgc cttaatgggt
     8161 gtatcaaaga aaggaacgag agcgtctctt aacgcgaagg aaaccgactg gatggaaaga
     8221 agagcgaccg cactgccgac gcctgcgaag attcttgcaa gggacaacag cttgattctg
     8281 tccttgtcgc tctcagtcat gagggctgta atgccccaaa ggggaacatc gcctgcggtg
     8341 tagaggatat cccaaaggaa gtatgccaga gccgcccaag cgacgaccgc aatattgcgt
     8401 aaagcggttg agggaaggga tgtatcatag aagccgttta agaagcagaa cattgttaca
     8461 aagaatatcg gcacaggcaa aaaaatgagg taaggtctgc atttgcccca ctttgttctt
     8521 gttcggtcta tgatatgacc cataacgggg tcattgattc cgtcccaaac cttaagcacg
     8581 gcaaggattc cgccgactat gcccgcggga atggccagaa caaactgcag ataatagcta
     8641 aatgtctggc ttatgatgtt atacattatg ttctgaccgg ccaagcctgc aaggtatgcg
     8701 ttgcgctctt ttttggtata ggtgttaagt tcaaccgact tgctcataag actcccccga
     8761 tatagtatta tttgtcatca aaatataagg ggacaaaagc cccctattat ttattactat
     8821 ctaaaagttc atcttaccag gaatgggatt ataaggtctt atccagcttt gcgcagtagc
     8881 gcaggatttt tcttgcgtcg ctggcgttaa ccttgccgtt gccgtccaca tccattgagt
     8941 gctcaaaggc tttgttggtg ctttgaagct ttgcgtatac tctgagtacc actcttgcgt
     9001 cggcgggctg caccttgccg ttgttgtcgg cgtcgccttt aatcgggcag acataaacct
     9061 taaagctggc cgacttgccg ttgtatgtaa cggtgatggt ctgctcgccc accttgtcct
     9121 tattgtagcc ggtgatggta tagcctgagg tgacctcaac ttcgccgctt gagcccgaca
     9181 tctttgctct aaccctcatt ccttttgtgt caagggcatt gccgactgtg ttgtaaaggt
     9241 ttgttggcgg ggtgactgaa attgatgtaa cgccggaggc tgcaacgacc tcaatgttat
     9301 ttctaagtac aatttccctg tttgttacgc cgtcgatgca gattaccctt agggttttca
     9361 ttccgggtgt cgaaaggtcg acaggctcaa ccttaaatct gttcggctgc cttccgcttt
     9421 catatcttaa cagaagctca gatgtaccgt ctctcctgta agctctgatg tttccgctct
     9481 ttaactcatt ggagaaagtg taattctttt tagtggggtc aactgtatat ttatcgggaa
     9541 tggacacttc aaatttaaga gaatcaatag gagcgggggt atcgggaatt atattgcttt
     9601 gtaccgatgc ggtaaccctg gagccgaaag tggcgggcct gagaacctca acaccgccgt
     9661 cgcttttctg gaaacctaca tattcgggcc ggatttcaaa attcattgcc tcgtaataat
     9721 ggaatttgcc gtcgaaagaa tcaggattcc tctttacata gaacagaaga tatgtaattg
     9781 aaccgccgct tgaagaaagc tgataataat cggtggtgaa gaaggcaaca tgaactctct
     9841 tgttatcgct gttggcaatg ctaatctcgt ttgaaaccgc taaatttccg tcatcgtcaa
     9901 gaatagaatc gctgttgctt acggcaccgc cgaattttaa ctttttgccg ttatattcaa
     9961 cctcaaagct gccgccgaca aatttgccgc tgccgcttac tcccactcta ataatcagcg
    10021 gaatgggata gacgccgtca gcctccgtgg gcaggtcctc gttcaaatcg cccaggaagc
    10081 tctcaagctc atctcttgaa agatttaact tagttacaga aaacttaatg ttgtaggtat
    10141 cctcagcaaa ggttgcaatt gggaaagctg taaacaacaa aaccgctacc aaagcaaccg
    10201 aaattgcgct tttaaatctg ttaagcattt tgtatgcccc ctatttactc gtggtttaat
    10261 tgtatcaaat tcggattgtc tttttcaagc gttttttatg aattattaat gaattgcaca
    10321 ataattgtat ggactgcact aacagttata tgttatattt acaaaaggag gaagcggatg
    10381 aaaattctac tatcggatga agtaaagtta gtgatagaca gattctctga gcacggctat
    10441 accgcctatg cggtgggcgg ctgtgtcagg gatctgctgc tttccttaaa cccgaaagac
    10501 tgggatgtcg cctcccccgc ttctcccgat gaaaccaagg aaatatttaa ggactttaag
    10561 gttctggata cgggcattaa gcacggaacg gttacggtga ttattaactc aatgcctatt
    10621 gaagtaaccg cattcagaac cgagggtgcg tattccgaca ggcgtcatcc tgatttcgtg
    10681 gagtttacaa aggatattga gtccgacctt aaaaggcggg attttaccgt aaacgccctt
    10741 gcctacaacc ctatcagggg gattgtggac atttccggcg ggctcgatga tttaaaaaat
    10801 aaggtcataa gaaccgtagg aaatcccaaa gaaagatttg acgaggaccc gctgaggata
    10861 ctcagagcct tgagatttgc ctcggctttg ggctttacaa ttgaagaaga aacagagaag
    10921 gcagtacaca aataccatcc gctgctgggg gagatagccg cggagagaat ttacgcggag
    10981 ctgaaggctc ttttttcggg tcagcgcatt gagtatgtac tgagcggctt taaagatgtg
    11041 gtcgcctttt tgttccctga gattaaggag agtctttatt cgggttatga ggctttgttt
    11101 ggggaagtga gccttaaatc cgaggaagta tttaaggcaa tatccctttt aagaggagat
    11161 ttttatttaa gggcggcgct tttgttctcg tcgggaaaag aggcggacgc ggaaatgata
    11221 ctgaataggc tcaggtccga taaggcgaca tcgggaaaag ttcaaaagct cgtaaaggct
    11281 cttaagctgc cgctccccag cacaaaagcc gatatgaaac gctttattaa cgctttcggc
    11341 tttgacgcag cggaggatta tctcattgcc gccgagaaag ttggttgtgc aattgaaagc
    11401 aaagcgctgc caaacctttc atcggcaaag tccttttttg agacgataag aaaaaacaaa
    11461 gaggcggtta caatacatga ccttgatata aaaggtacgg atattcagaa tctgggcttt
    11521 aagggagctg aggtcggcaa agctctcgaa attctgctcg atgccgtatt tgaggaagaa
    11581 tgcgaaaaca caaaagtgga tttaattcgg tttattaaaa accgttcgga ggatataaaa
    11641 cacaaataaa tgggaggtat ggatttgaga aaggttatta atttaaactt tggatggaag
    11701 tactcccccg tctttgaaga cgaaatgctg aaaaaaagct ttgacgacag ctcttttgaa
    11761 actgttgaca ttcctcacgc caacaaggag ctgccactta attatttcga tgaacgggaa
    11821 tatatgttcg tcagctgtta cagaaagaaa ttcaagctgg ataaaggaga ctatgcggac
    11881 ggcaagaggg tgctccttca ttttgagggc gttgcctgcc gctcccttgt gtatgtaaac
    11941 ggcaagtacg ccggagaata caagggcgca tatactccct ttacctttga cattaccgac
    12001 cacctgtcct caagggataa tgtgattgcg gtaagggtcg actctaccga aactcccgat
    12061 atgcccccct tcggcaaggt tgtggattat ctttgctacg gcggcattta cagagaggtc
    12121 tggcttgagt gcgtggaaaa agagcatatt gaggacatct ttgtaaggac cggcaatgtg
    12181 cttgacaaaa agaagctcct tacggcggac attacattct cagataaact gaagggcgaa
    12241 ttgaagctcg agcttttgga cggagaaaag gttatagcgg agagaaccac cgagtttgac
    12301 gccaaggtat tcagggtcaa atggggcatt cagggcgttc agaactggga tatcgaaaat
    12361 cccaagctct acacccttag agtcactttc ggcaaggatg ttaaggaagt aaggttcggc
    12421 tttagagaag ccagatttac aaagaacgga ttctatctta acggcaagcg tattaagctc
    12481 ataggtctta accgccatca gagctatccc tatgtgggct acgcaatgcc caaatccgct
    12541 caggaggcag acgcagacct tctcaaattc accctgggct gcaatatagt caggacctcc
    12601 cactaccccg tttcaaagca tttccttaat agatgcgatg agattggcct gcttgtcttt
    12661 accgagatgc ccggctggca gtacaccgac atgaacccgg gcgaatggca ggataacgcc
    12721 attgaaaatg taaggcgaat gataatcagg gacaggaacc acccctcaat agtgctttgg
    12781 ggcgtcagaa tcaatgaagg cgccgactgc gacgctttgt atgaaaagac aaacgccctt
    12841 gcaaggagcc ttgaccccac aaggcagacg ggcggagtca ggaacttccc gcaaagccat
    12901 ctgcttgagg atgtttatac ctataacgac ttctcacact ccggcggcaa aataaagctc
    12961 ctgcccccga cggttgtagc gggtccgaac gctccctatc ttgtaaccga attcaacgga
    13021 cacatgttcc ccacaaagag ctacgacagg gaggacataa gaactgagca cgctttaagg
    13081 cacgcgaggg ttcagtcagc gtcattcggc aacgacagga tttcgggcgc catcggctgg
    13141 tgtatgtccg actacaacac ccactgcgac ttcggcagcg gcgacaggat ttgctatcac
    13201 ggagttacgg atatattcag aatcccgaag ctcgccgcgg cggtttacgc ctcccagcag
    13261 gattacatcc ccgttcttga ggtatcctcc tcaatggata taggcgacca tcccaagagc
    13321 ttcaggggtc agatatatat cttcacaaac tgcgactatg taaaggttta taagaac
//
LOCUS       DAONVQ010000044        13186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3603_length_13186_cov_4.324423, whole genome shotgun sequence.
ACCESSION   DAONVQ010000044 DAONVQ010000000
VERSION     DAONVQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13186
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3603_length_13186_cov_4.324423"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PLD98_04195"
     CDS             complement(<1..1149)
                     /locus_tag="PLD98_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA05484.1"
                     /translation="MSRKITFGIIGVGNMGSSHMRSFMEGKLPELELTAVADINPERL
                     EWAKEQLPNISVFNTASELIHSGKCEAIVIATPHYDHPPIAIEALKAGLHVMTEKPAG
                     VYTKQVREMIEVASQTDRTFAIMFNQRTNVVYRKLRELVQNGEYGEIRRVNWIITDWF
                     RTQAYYDSGGWRATWAGEGGGVLINQCPHNLDLWQWICGMPNKIWATCHQGKWHNIEV
                     EDDVTIYAEYPNGATGVFIATTGDCPGTNRLEITMDKAKIVCENNEIKLYVLDESISK
                     FTYEAKLGFAKLKGQYVDVEITGESTHHPGVLNAFAANILRGEPLYAHGSEGINSLMI
                     SNAAYLSSWLGRPVELPLDEDLFYEELKKRIESSNAKKEVKAQVQEDMS"
     gene            1488..1610
                     /locus_tag="PLD98_04200"
     CDS             1488..1610
                     /locus_tag="PLD98_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05485.1"
                     /translation="MEQIMEYIKRFFEILREFLKLIGVELPDFFDTTKAPAEED"
     gene            complement(1744..1872)
                     /locus_tag="PLD98_04205"
     CDS             complement(1744..1872)
                     /locus_tag="PLD98_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05486.1"
                     /translation="MSLYTIIAMLQWAISMIEKFMLQYLGWSGWETTAPESESESE"
     gene            complement(1960..2871)
                     /locus_tag="PLD98_04210"
     CDS             complement(1960..2871)
                     /locus_tag="PLD98_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQA05487.1"
                     /translation="MTLIQFLLIALSVACGVLFSSTRSRFSKKHVTELSDLMIFNSLC
                     AVVASVLLGLVTLITNNEINSFTLVFGLIYGVMITAGSLTALMAFKFGPLSYTTLITA
                     FAMVIPSVAGVLFWNESIKPIKYLGMAMILVSVFFAVDRKKTDEKKTSLKWLFFCILT
                     LFINGSYGVMQKRFYMRENGGGTYAFLMISFIVSAIATFIAYGILSRRKKQNPPEKRL
                     GKSDLFKRVLIIFSLICGSTTVVNNIINTYLAGTMDSAVFFPVLNGCSLYTTIIVSVV
                     IFKERLAKRQWFGLIVGFAAIMLLALS"
     gene            complement(2892..4268)
                     /gene="purF"
                     /locus_tag="PLD98_04215"
     CDS             complement(2892..4268)
                     /gene="purF"
                     /locus_tag="PLD98_04215"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQA05488.1"
                     /translation="MNKSKYSLGEECAVFGFFNRGEKDVGQLAYQSLFSMQHRGQDGA
                     GLAGAVGNEVRHHKNIGLVNEVFTKQILDEYANDEILIGHVRYATSSSVTVLDTQPLV
                     MHGYEGFIAMAHNGHIVNGNEIRRELQKNGVLFQTNVDSEALMHIIAGNRDGIEEGIK
                     DLIKRVRGSYALTLMCPGKLIGVRDPWGIRPLCLGRLGDSYVLASESCAFNAIGAVFV
                     RDIKPGEIVIITSSGISSIETPPMERTALCVFEYVYFARNDSVIDGVNVYQSRIRMGR
                     HLCKTAPVEADIVSGVPESAIPAAIGYANESGITYGQCLIKNAYSGRTFIQEGQMNRE
                     QSVRMKLCAIHENVENKRIVLVDDSIVRGTNSMYIVKMLRNAGAKEIHMRIASPPVKF
                     ACRFGIDTPSRKNLVGAYLDTEEIASRIGADSLAYLGIEDLIAAVGNKNLGLCSACFD
                     GKYPMPID"
     gene            complement(4261..4974)
                     /locus_tag="PLD98_04220"
     CDS             complement(4261..4974)
                     /locus_tag="PLD98_04220"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQA05489.1"
                     /translation="MQKGAMLYEGKAKKVFATDEPQLVIVEYKDDATAFNGLKKGSIQ
                     GKGAINNRLSNHFFRILETEGVPTHYAEELSDRETLVKKVQIVPLEVIIRNITAGSLS
                     KRFGVEEGIVLDTPIFELSYKNDDLGDPMINHSQAIALKFTTKEELEKIESMAMKVNE
                     VLKREFLKANLILVDFKLEFGKTEEGEIVLADEISPDTCRLWDTDTKEKLDKDRFRRD
                     LGGVEEAYIEVARRVLGNE"
     gene            5228..5938
                     /locus_tag="PLD98_04225"
     CDS             5228..5938
                     /locus_tag="PLD98_04225"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HQA05490.1"
                     /translation="MSKNVAVILAGGSGNRFGESIPKQFLEVEGKTILEYSMLALQRH
                     PEIDEFSVVVSREYFEKVKSLAEKNGCTKFGKTIAGGSERYESSLNAVRAYEDTPDIN
                     ILLCDSARPVLRQRYITEVIEALKHYNAATIAVPEIDTVLRVNPETMLMTQRLDRNEI
                     FRAHTPQGFKYDVLRKAYEIGLKDPDFNPTDDTGVIFKYLPDEKVYIIKGEHDNIKLT
                     YKQDLEIIRKALKCLENE"
     gene            complement(5967..6818)
                     /locus_tag="PLD98_04230"
     CDS             complement(5967..6818)
                     /locus_tag="PLD98_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HQA05491.1"
                     /translation="MNASKKNALKIAALVIAGILVAGAAYAVVSTYLYESKKKPIVPS
                     GKTLDLTGYELVFEDDFDGDELDLEKWQYRANGKRRAGFNSPNQVTLKDGNLHIKAEY
                     KEDGEYGPGWYTGMIRTNSEYITGYFEVRCKANEGGGFWSAFWLNSQGMNAQSNGGIN
                     GAEIDIFEAFNYDKKTYFFDIRDSVSLNIHVDGYGEELKSKNLGNFYVKDPYNEFHTY
                     GLEWTTSEYIFYIDGVEAVRSSFDKGVSKALEYAILSLEPPSEVTAKPGFETEFIVDY
                     VKIYQKK"
     gene            6953..7261
                     /locus_tag="PLD98_04235"
     CDS             6953..7261
                     /locus_tag="PLD98_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HQA05492.1"
                     /translation="MIVTTTGSIEGRKITGYKGIVFGEVILGVDFVKGIDAGIRNFFG
                     GRSQAYEQEIIAARQKAINEMCQNAFNMGANAVIGVKIDYEQITGMLMVTASGTAVVV
                     "
     gene            complement(7300..8715)
                     /locus_tag="PLD98_04240"
     CDS             complement(7300..8715)
                     /locus_tag="PLD98_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQA05493.1"
                     /translation="MSLKTSLSESALNKLADTLLNYLNKDPEKNILKVLDKVKIVFTS
                     GMFPKENYMKMREAAKNPNNTYVKLIKNILRDVDQNIIRQLVIALGYHAAYLGTKTVR
                     ANREKYNCNIPWVILLDPTSACNLNCKGCWSAEYGHKENLSLEEMMDIVKQGKALGTH
                     FYMFTGGEPLIRKNDIIKLCEQNPDCAFLSYTNGTLVDDAFCAEMRRVGNLALALSIE
                     GTEESNDARRGKGVYQKTLQAMDILKKNRCLFGISVCYTRDNIPYVTSDEFLDLMIEK
                     GVKFAWYFNFMPVGRTADPELIPTPEQRKFMYSWIRKVRSSTEGKPMFAIDFQDDGEF
                     VGGCIAGGRNYFHINSAGDIEPCVFIHYSDSNIREKTLLEALQSPLFMAYRKNQPFND
                     NHLRPCPMLENPDALRRIVRETGAKSTNLMEPEDVETLCAKCDRFAKEWAPVAEEIWN
                     STTHPKTFTQYYRDNGKGKRQ"
     gene            complement(8886..9740)
                     /locus_tag="PLD98_04245"
     CDS             complement(8886..9740)
                     /locus_tag="PLD98_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQA05494.1"
                     /translation="MEKKVKITTDSPADLPPELAKRYDISIIPLHIILGGESYDDGVN
                     ITPSDIYRVYDERKELPKTSAVTIGEYQDFFKQFADEGYEVVHLSLSSGISSTHANAV
                     LAAKKFDGVYVVDSKLLSVGIALLCIKAAEMRDQGISAAEIYERIHNLREKVVCDFIL
                     DNLEFLRKGGRCSALQAFGANVLGIKPSIVMQDGTLSVGKKYRGKTEICFTQYVNDKL
                     SEYEGKIDTDRIFVHHTEGVSQQFMDNVKKELKKRLKPKEIIQSEAGCTITAHCGKRT
                     FSLMFMAK"
     gene            complement(9929..10660)
                     /locus_tag="PLD98_04250"
     CDS             complement(9929..10660)
                     /locus_tag="PLD98_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05495.1"
                     /translation="MKKKLYDITPIPMLHQIIWWVVRGLLLIFAIHGLFTNSVTEFLM
                     GMFSIAFSHLWDMFQLFGGRSFISRVSYSSQTLLNIFIFFGCVVGPYLNTKTQFQYSD
                     IILHFTSGIVATWFGYDFAETIQGKHKPISPALAALFCLGFSISIAVGWEFYEFTMDR
                     LYGYTLQTSDILSERGLVDTMVDFIMAAAGSVVGMFIVSFTKNGIIGPNRKEIRARVK
                     EEYKKHKELELKFLEEQKKAKKQSK"
     gene            10929..12611
                     /locus_tag="PLD98_04255"
     CDS             10929..12611
                     /locus_tag="PLD98_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HQA05496.1"
                     /translation="MAAEIKKSWYKEMVVYQIWPRSFCDGNGDGIGDLKGILSKLDYI
                     KSLGVDAIWFSPLYKSPQKDYGYDVADYRDIAPEYGTLEEFKLVLEEAHKRGINVIMD
                     LVISHTSDQHYWFLESKKGEDNPYHDYYFWRKGRLGGKLPPNNWKSVFSEPGWKYDEN
                     LDKWYLHLFATEQPDLNMDNPKVREEVKDIMRFWLDMGVDGFREDVITFISKAEGLPN
                     GFPIPVATGIEHYMDGPHLDEYLAEFRKVIDEYDCVTIGEAPMMTPKRALKYITEGPD
                     QKLHMMFHFQHMEADCLFISWIRRPFSLKKLKKAFTNWQEQLYGKAWNALYIENHDQP
                     RVINRYGSLKYREESAKMLAVMYMCQSGTPFIYQGQEIGMTNIELPSIDMYDDISTIN
                     NYKLARKLGLKESTVMKIVNYASRDNSRTPVQWSAEKNAGFTTAEKAWFHINPNYTEI
                     NVEAAEKDPNSILNFYRKLIKFRKENEIVIYGSYKEHYKDSDELYVYSRYYEGKKMLV
                     VCSFSEKPVSFKAPEGFDLALGELKLSNYDNCGIANNGFVLRPYETRVYLFS"
     gene            12655..>13186
                     /locus_tag="PLD98_04260"
     CDS             12655..>13186
                     /locus_tag="PLD98_04260"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQA05497.1"
                     /translation="MSVICKEAYYNSSTGKDTIRALIWHDDTVAPIGVFQIAHGVCEH
                     IGRYDDFARFLASNGFVVCGNDHLGHGKSVKSLADLGHTADENGDVRMVDDMHILTCI
                     MKKRYPNLPYFLFGHSMGSFAARIYAADFGHELDGVIFCGTGQIPGVAALLKDAVEKT
                     IDKIGVRKSSLGTMQLL"
BASE COUNT         3536 a         3111 c         2990 g         3549 t
ORIGIN      
        1 gctcatgtcc tcctgaacct gagcctttac ctctttcttg gcgttggagc tttcgattct
       61 ctttttcagc tcctcataga agaggtcctc gtcaagaggc agctcaacgg gtctgccgag
      121 ccaggaggag aggtaagcag cgtttgaaat catcaggctg ttaatgccct cagaaccgtg
      181 agcgtaaaga ggctcgccgc ggaggatgtt tgccgcgaaa gcgttgagaa cgccgggatg
      241 atgtgtgctc tcgcctgtga tttcaacatc aacatactga cccttaagtt tagcaaagcc
      301 cagctttgcc tcatatgtaa acttggaaat gctctcgtca agaacataga gcttaatctc
      361 attgttctcg caaacgattt ttgccttatc cattgtgatt tcaagcctgt tggtgccggg
      421 gcagtcgcct gttgtagcga tgaatacgcc agttgctccg ttgggatact cggcgtagat
      481 ggttacatcg tcctcaacct caatgttgtg ccacttgccc tgatggcatg tagcccaaat
      541 cttgttgggc ataccgcaaa tccactgcca aaggtcaagg ttgtgggggc actggttgat
      601 aagaacgccg ccgccttcgc ctgcccatgt ggctctccat ccgccggaat catagtaagc
      661 ctgagtgcgg aaccagtcgg tgattatcca gttaactctt ctaatctcgc cgtattcgcc
      721 gttctgtacc aactcgcgca gttttctgta aaccacattg gtcctctggt tgaacatgat
      781 tgcgaatgta cggtcggtct gggaggcaac ctcaatcatc tctctgacct gctttgtgta
      841 aacgcccgcg ggcttttctg tcatgacatg aagtcctgct ttgagtgcct caatggcaat
      901 gggcggatgg tcatagtgag gagttgctat gactatagct tcacacttgc cgctgtgtat
      961 aagctcggaa gcggtattga aaacgctgat gttcggcagc tgctcttttg cccattcaag
     1021 cctttcggga ttaatatccg caacggctgt gagctcaagc tcgggcagtt ttccctccat
     1081 aaatgagcgc atgtgcgagg aacccatgtt acccacaccg ataatgccga aagttatttt
     1141 tcttgacata atcagatccc ctttgctttt aattaaacac gcagagttta aagtcgctgt
     1201 aattatacca cagggatttt tttcattcaa caaaaaaagg agaattaaaa gaatatttgg
     1261 ggcgatgaaa gcacatttag agcgaatcca taatatttcg tgtttgaata tatattttcg
     1321 atacggaaaa atggaaattt tgtttttggt attgcaggca tattgcaaaa atttgttatt
     1381 aatataaatt tttatcaaaa tacataaaaa tccttgtaat gacatatctt aaatgttaat
     1441 atcaagatac caaaaatagt aaaagctatg aaagagaggt gctaataatg gagcaaatca
     1501 tggagtacat caagagattc ttcgagattt taagggagtt cctcaagctg attggcgttg
     1561 agctgcccga tttcttcgac acaaccaagg cccccgcaga agaagactaa ttagttaagc
     1621 ccgagaaggt cacagcaaaa gctgtggcct tctttttttt atgcggacat tcctttgcgt
     1681 cggcatgcta gagtttctaa cgcaaaaaat ctccgggagc ttgtcccgga gatttaggct
     1741 tttttattcg gattcggatt cgctttcggg ggctgtggtc tcccagccgc tccagccaag
     1801 gtattgaagc ataaactttt caatcattga gatagcccat tgcagcattg caataattgt
     1861 gtaaagtgac atactacccc tccttatctt tgacagtcca aaatcatatt agcacatcca
     1921 ttaaatgatt acaatataca aaatgtattt tgtgaatatt tacgaaaggg caagaagcat
     1981 gattgccgca aagcctacaa tcagaccgaa ccactgccgt tttgcaagtc tctctttaaa
     2041 aatgacgacg gatacgatta ttgtcgtata aagcgagcag ccgtttaata cggggaagaa
     2101 caccgcgctg tccatagtgc ccgcaaggta agtgtttatg atgttgttca caaccgtcgt
     2161 actgccgcag attaaagaga aaatgataag tacccttttg aacaaatcgg atttgcccag
     2221 tcttttctcc ggcgggtttt gctttttgcg ccttgacaga atcccgtagg cgataaaggt
     2281 tgcgatggcg gatactataa aggaaatcat taaaaaagcg tatgttccgc cgccgttttc
     2341 ccgcatataa aaccttttct gcataacgcc gtacgagccg ttgataaaaa gggtaagtat
     2401 gcagaaaaag agccatttga gcgatgtctt tttctcatcc gtctttttgc ggtcaactgc
     2461 gaaaaatacg gagacgagta tcatcgccat gccaaggtac ttaataggct ttatgctctc
     2521 attccagaac agcacgcccg cgacggaggg tatgaccata gcgaaggcgg taatgagcgt
     2581 ggtgtaggag agtgggccga acttaaacgc catcaaggcg gtaagcgagc cggcggtaat
     2641 cattacgccg tagattaagc caaaaaccaa agtaaaggag tttatctcat tattagtgat
     2701 aagcgtaact aagcctaaaa gtaccgatgc tacaaccgcg cagagagagt tgaaaatcat
     2761 caggtccgaa agctcggtca catgcttttt gctgaacctg cttcttgtgg acgaaaacag
     2821 tacgccgcat gccaccgaca aggcaatcaa taaaaactgt attaaggtca tgttttaccc
     2881 tttcgtcgct gttaatcaat gggcataggg tacttgccgt caaagcaggc gctgcaaagt
     2941 cccaaattct tgttgcctac cgccgcgata aggtcctcaa tgccaagata agcgagagaa
     3001 tccgcgccaa tcctgcttgc gatttcctct gtgtcaagat atgctcccac aaggttcttt
     3061 cttgagggag tatcaatgcc gaagcggcag gcgaatttaa cgggcgggga ggcgattctc
     3121 atatgtattt ctttggctcc cgcgtttctc agcatcttaa ctatatacat gctgtttgtg
     3181 cctctgacta tggagtcgtc aaccagcact attctcttat tctcaacatt ttcgtgtatt
     3241 gcgcagagct tcattctaac gctctgctca cggttcatct gaccctcctg aataaaggtg
     3301 cgtcccgaat acgcgttctt tataaggcac tgaccgtagg taatgccgct ttcgttagcg
     3361 tagccgatag cggcggggat ggcggattcg ggaactcccg acacaatatc cgcctctacg
     3421 ggagcggttt tgcacagatg ccttcccatg cggattcttg actgatatac atttaccccg
     3481 tcaataacgc tgtcgttgcg ggcaaagtac acatactcaa aaacgcaaag ggcggttctt
     3541 tccataggag gagtttcgat ggaggaaatt cccgaggagg tgattataac aatctcaccg
     3601 ggcttaatat cccttacaaa tactgctcct atagcgttaa aggcgcagct ttccgaagcg
     3661 aggacataag agtcgccaag tctgccgaga cacaaggggc ggatacccca ggggtccctg
     3721 actcctatca gctttccggg acacataagc gtcagagcgt atgagcctct gacccttttt
     3781 ataaggtcct ttatgccctc ctcaatgccg tcacggttgc ccgcgataat gtgcattaaa
     3841 gcctcgctgt ccacatttgt ctgaaacaga acgccgttct tttgcagctc tcgcctaatt
     3901 tcgttgccgt tcacaatgtg gccgttgtga gccatagcga tgaaaccttc gtatccatgc
     3961 attacaaggg gctgagtgtc aagaacggtc acacttgagc ttgtggcgta gcggacatgt
     4021 ccaataagta tttcgtcatt tgcgtattcg tcaagaatct gctttgtaaa aacctcgttt
     4081 acaagtccta tgttcttatg gtgacgaacc tcgtttccca cagcgcctgc aagacccgcg
     4141 ccgtcctgac ccctgtgctg catggagaaa agggactgat aggcaagctg tcccacatct
     4201 ttttcgccgc ggttaaaaaa gccgaaaacg gcgcattcct cacccaaaga atacttactc
     4261 ttattcattt ccaagtaccc ttcttgctac ctcgatatat gcctcttcaa cgccgccgag
     4321 gtctcgtctg aaacggtcct tgtccagctt ttccttggta tctgtgtccc aaagcctgca
     4381 tgtatcggga gaaatctcgt cagcaagaac aatttcgccc tcttccgtct ttccgaactc
     4441 cagtttaaag tcaaccagta taaggtttgc tttaaggaat tctctcttta aaacctcgtt
     4501 taccttcatt gccatggact cgattttttc cagctcttct tttgtggtaa actttaaagc
     4561 tattgcctgg ctgtgattta tcatcgggtc gccaaggtcg tcgttcttat agctcaattc
     4621 aaatatagga gtgtcgagaa caataccttc ctcaacgccg aaacgcttgc tgaggctgcc
     4681 tgcggttata tttcggataa taacctcaag gggaacgatt tgaacctttt taacaagggt
     4741 ttccctgtcg ctgagttcct ctgcatagtg ggtgggaacg ccctcagttt caagaattct
     4801 gaaaaagtgg ttcgaaaggc ggttgttaat agctcccttt ccctgaatgc tgcccttttt
     4861 cagtccgttg aaagccgtcg cgtcgtcctt gtactccact atgacaagct gaggctcatc
     4921 tgtggcaaaa accttctttg cctttccttc gtaaagcatt gcgccctttt gcataaacat
     4981 cctcctacaa aaatattccc gagtattcag ctacataagg attatacctt attaaggtaa
     5041 taatgtatat atgctttaaa gcggtaaaaa cgcagaaaaa aagcccctta gagccttcat
     5101 ttttatacat ttccaccgca tttttactta atagataaat tggcagaaaa agctcgaatc
     5161 gtggagattg actgcgaaaa tcataaggct tataattaat aaaaagagtt ccgaaacgga
     5221 ggataatatg tctaaaaatg tagcggtcat tttggcaggc ggcagcggca acagattcgg
     5281 ggagagcatt ccaaagcagt ttttggaggt agagggcaaa accatactcg agtattcaat
     5341 gctcgcgctt cagcgtcacc ccgaaatcga tgaattctcc gtggtggttt cgagagagta
     5401 ttttgaaaag gtaaaaagcc ttgccgaaaa aaatggctgc accaagttcg gaaaaacaat
     5461 agcaggcggc agcgaaaggt atgaatcctc ccttaacgcc gtcagagcat acgaggatac
     5521 ccccgatata aatatccttt tgtgcgacag cgcaagaccc gttttaaggc agagatacat
     5581 taccgaggtt atagaggctt taaagcacta taacgccgca accatagccg ttcccgaaat
     5641 tgacacagtg ctgagggtca accccgaaac catgcttatg acccaaaggc ttgacaggaa
     5701 cgaaatattc agagcccaca cgccccaggg atttaaatac gatgttctga gaaaggctta
     5761 tgagattggt ctaaaagacc ccgactttaa ccccaccgac gatacgggcg tgatattcaa
     5821 atacctgccc gatgaaaagg tatatattat aaaaggtgag cacgacaata ttaagctgac
     5881 atacaagcag gatttggaga taatcagaaa agctctgaaa tgtctggaga atgaataaaa
     5941 aaccgcctca aaagaggcgg cttttttcat ttcttctgat aaatctttac atagtccaca
     6001 ataaactcgg tttcaaagcc gggctttgcc gttacttcac tgggcggctc aagggataaa
     6061 atcgcatact caagagcctt tgaaacgccc ttgtcaaagg aggagcggac agcctccacg
     6121 ccgtcgatat agaatatgta ctcgcttgtg gtccactcaa gaccgtaggt atgaaactca
     6181 ttgtagggat cttttacata aaagttgccc agattctttg atttcagctc ctcgccgtag
     6241 ccgtcaacat ggatgttgag ggaaaccgag tcccttatat caaagaagta tgtctttttg
     6301 tcgtaattga aggcctcaaa aatgtctatc tcagcgccgt tgattccgcc gtttgactgg
     6361 gcgttcatgc cctgagaatt aagccagaag gcgctccaga agccgccgcc ttcgtttgcc
     6421 ttgcagcgca cctcaaagta gccggttatg tactcggaat tggttcttat catgcccgtg
     6481 taccagccgg gaccgtattc gccgtcctct ttatactccg cctttatatg cagatttccg
     6541 tctttgagcg ttacctgatt cgggctgtta aagcctgcgc gccttttgcc gttggctctg
     6601 tattgccatt tttcaaggtc cagctcgtcg ccgtcgaaat catcctcaaa aacaagctca
     6661 tatcccgtaa ggtccaaagt tttgcccgat ggaactatgg gcttcttttt gctttcgtac
     6721 aaatatgtgc tgaccacagc gtaagcagct cccgcgacaa gaattcccgc aataacaagc
     6781 gcggctattt taagagcatt ctttttagag gcgttcatac gaaaactcct tcaataatat
     6841 tttgctaaat attagcatat accctatatt tattcaataa tatgaagtca ttttcttgca
     6901 cggagattaa aacgggttta taatgagaaa aagacaaaaa ggggagagaa aaatgattgt
     6961 taccaccaca ggcagcatag agggcagaaa aattaccggc tataaaggca ttgtgttcgg
     7021 cgaggtaatt ctcggcgttg attttgtaaa aggcattgac gcgggtatca gaaatttctt
     7081 cggcggccgc tctcaggctt atgaacagga gattattgcg gcgcggcaga aagccataaa
     7141 cgaaatgtgc caaaacgcct tcaacatggg tgcgaacgct gtcatcggag tgaaaataga
     7201 ctacgaacag attacgggca tgctgatggt taccgcgtcg ggcaccgctg ttgttgtcta
     7261 ataaaaaaag ccgctttaaa agcggcttta atttttgttt tactgcctct tgcctttgcc
     7321 gttgtccctg taatactggg taaaggtctt gggatgggtt gtggagttcc aaatctcctc
     7381 cgcgacggga gcccactctt ttgcgaagcg gtcgcacttt gcgcaaaggg tttccacatc
     7441 ctcaggctcc atgagatttg tggacttagc gcccgtctca cgaacgattc tgcgcaaagc
     7501 gtcgggattt tcaagcatcg ggcaggggcg cagatggttg tcgttaaacg gctgattctt
     7561 tctgtaagcc atgaacagcg ggctctgcag agcctcaagc agcgtctttt ctctgatatt
     7621 cgagtctgaa tagtgaataa atacgcaggg ctcaatgtcg ccggcggagt taatgtggaa
     7681 gtagtttctg ccgcctgcga tgcagccgcc cacgaactcg ccgtcatcct ggaagtcaat
     7741 agcgaacatc ggcttgccct cagtggagct gcgaaccttt ctaatccatg aatacataaa
     7801 tttacgctgt tcgggagtgg ggataagttc cgggtccgct gtcctgccga ccggcataaa
     7861 gttaaagtac caagcgaact taacgccctt ttcaatcata aggtcaagaa actcgtcgct
     7921 tgttacatag gggatattgt cgcgtgtgta gcaaacggaa attccgaaaa ggcacctgtt
     7981 tttcttgaga atatccatgg cctggagggt cttttgatat acacccttgc cgcgccttgc
     8041 gtcgttggat tcctcggtgc cctcaatgct gagagcaaga gccagattgc caacccttct
     8101 catttcggcg cagaatgcgt cgtcaacaag ggtgccgttg gtgtagctta agaatgcgca
     8161 gtcgggattc tgctcgcaca gctttataat gtcgtttttt ctgataagcg gctcgccgcc
     8221 cgtgaacata tagaaatggg tacccagagc cttgccctgc tttacaatgt ccatcatttc
     8281 ttcaaggctc agattctctt tatgaccgta ttccgcggac cagcagccct tgcagttaag
     8341 gttgcacgcg cttgtggggt caaggagaat aacccacgga atgttgcagt tgtatttttc
     8401 tctgtttgcc ctgaccgttt ttgttccaag ataagcagcg tgatagccga gagctattac
     8461 aagctgcctt atgatgttct ggtcaacatc ccttagaatg ttcttaatga gcttgacata
     8521 ggtgttgttg ggattcttgg ctgcttccct catcttcatg tagttttcct tgggaaacat
     8581 tccgctggtg aaaactatct taaccttgtc aagaaccttg agaatattct tttcagggtc
     8641 tttatttaaa tagttcagca gagtgtccgc caatttgttt aaagcggatt ctgacaaact
     8701 cgttttaagt gacattttaa aatcctccaa aaaaagttgc atgtcgtcga gtaatataca
     8761 aattatagca cgctggcaaa tgtttgtaaa ctcaaagatt tcagtatggt ttgaagaaac
     8821 taactcctat cttaatagcg taatgtctgg aggatttacg gtaattaaga taacggaaaa
     8881 cagactcatt tcgccatgaa cataagcgag aatgtcctct tgccgcagtg ggctgtaatg
     8941 gtgcagcccg cctcgctttg gataatctct ttgggtttca gtctcttttt aagctctttc
     9001 tttacattgt ccataaactg ctggcttacg ccctcggtat ggtggacaaa aatcctgtcg
     9061 gtatcaattt tgccctcgta ctctgagagc ttgtcgttta catactgcgt aaagcagatt
     9121 tcggtcttgc ctctgtattt ttttccgacc gaaagggtac cgtcctgcat aactatagac
     9181 ggcttgattc cgagcacatt cgctccgaac gcctggagag cggagcagcg tccgcctttg
     9241 cgcagaaact ccagattgtc aagaataaag tcgcagacta ctttttctct tagattgtgg
     9301 attctttcat aaatctcagc cgcgcttatt ccctggtctc tcatctcggc agcctttatg
     9361 cacagcaagg cgatgcctac tgacaaaagc tttgagtcaa ccacataaac gccatcaaat
     9421 ttctttgccg cgagcactgc gttcgcgtgg gtggatgaga tgccggaaga aagggagaga
     9481 tgtactacct catagccttc atccgcaaac tgcttaaaaa agtcctgata ttcgccaatt
     9541 gttacggcgc tcgttttggg cagctccttg cgctcgtcat agaccctgta aatatcgctg
     9601 ggagtgatat ttactccgtc gtcatagctt tcgccgccca gaatgatgtg cagaggaata
     9661 atgcttatat cgtatctctt ggcaagctcc ggcggcaaat ctgccgggct gtcagtcgtt
     9721 attttaacct tcttttccat ttctcttcct ccggttcctg agctgtcagt ataatatatt
     9781 atacctaatc agcccatatt tctcaatttt aatatattta ataatatgcc ctaaactgtg
     9841 aataaattgt gaagtcaaag ttaatttccg agtaagaaaa aatattaagg cggataaatt
     9901 aatagcggca aagaaattgc cgctaatttt attttgattg tttctttgct ttcttttgct
     9961 cctccaggaa cttaagctcc agctccttat gctttttgta ttcttccttg acccttgccc
    10021 ttatctcctt gcggttggga ccgataattc cgtttttcgt aaagctcact atgaacattc
    10081 cgacgaccga gccggcggca gccataatga agtctaccat agtgtccaca agacctcttt
    10141 cgctgagaat atccgaggtc tggagcgtat agccgtagag cctgtccatc gtaaactcat
    10201 aaaactccca gccgacagcg atggaaatcg aaaagccgag gcagaacaaa gcggcaaggg
    10261 cgggacttat gggcttgtgc tttccctgaa tggtctcggc aaagtcatag ccgaaccatg
    10321 tcgccacaat gcccgatgta aagtggagga taatatccga atactgaaac tgcgtcttag
    10381 tattaagata gggacccaca acgcagccga agaaaatgaa gatgttcaat agtgtctgcg
    10441 atgaatatga aacccttgaa ataaaagacc ttccgccaaa gagctggaac atatcccaaa
    10501 gatgcgagaa ggcgatggag aacatgccca tcaaaaactc cgttacagag tttgtaaaaa
    10561 gcccgtgaat cgcgaagatg agcaaaagtc ctctgacaac ccaccagatt atctggtgca
    10621 gcatcggaat aggggttata tcataaagct tctttttcat ctggagccgt attcctttcg
    10681 ttctatattc atttcgacac cgaataataa tatcataagc cgctattatt ttcaattaaa
    10741 aaccaattct gtaatcctca gttttctcat attgaaaaat tttgtaagag tgcataattt
    10801 ctcagccttt ttttgttaat tcctttacat aattatataa aaatgctaaa atgtttcaga
    10861 tgtccaatgt aaaacagtca gggcaataac gccttgctca ttatactacg aaaggtgttg
    10921 cttcttgtat ggctgctgaa ataaagaaaa gttggtacaa agagatggtc gtctatcaga
    10981 tttggcccag gagcttttgc gacggcaacg gcgacggcat cggcgacctt aaaggaattc
    11041 tctcaaagct ggactacata aagagccttg gcgttgacgc catttggttc tcaccccttt
    11101 acaagtctcc ccaaaaggac tacggctatg atgtcgccga ctacagggac attgcccccg
    11161 aatacggcac tcttgaggaa ttcaagcttg ttttggaaga ggcacacaaa aggggcatca
    11221 atgtcattat ggaccttgtc ataagccaca catccgacca gcattactgg ttccttgaaa
    11281 gcaaaaaggg cgaggataac ccctatcacg attattactt ctggcgcaaa ggaagactgg
    11341 gcggcaagct cccgcctaac aactggaagt ccgttttctc cgaacccggc tggaagtacg
    11401 acgaaaatct tgataaatgg tatttgcacc tgtttgccac cgagcagccc gacctgaaca
    11461 tggataaccc caaggtcaga gaagaagtaa aggatattat gcgcttctgg ctcgacatgg
    11521 gcgttgacgg tttcagggag gatgtcatta ccttcatttc aaaggcggag ggtctgccca
    11581 acggcttccc gattcccgtc gctactggca ttgagcacta tatggacggc ccccatcttg
    11641 acgaatacct tgccgagttc agaaaggtta tcgacgagta tgactgcgta accataggcg
    11701 aggctcccat gatgactccc aagagggcgc tgaagtacat cactgagggt cccgaccaaa
    11761 aactgcacat gatgttccac ttccagcaca tggaggcgga ctgcctgttt ataagctgga
    11821 ttagacgccc cttcagcctt aaaaagctga agaaagcttt caccaactgg caggagcagc
    11881 tttacggcaa ggcatggaac gcattgtata ttgaaaacca cgaccagccg agagtcatca
    11941 accgctacgg cagcttaaag tacagggaag aaagcgcgaa gatgcttgcc gtcatgtata
    12001 tgtgccagag cggcaccccc tttatctatc agggtcagga aatcggcatg acgaacatcg
    12061 agctgccctc catcgatatg tacgatgaca tatccacaat aaacaactac aaactcgcaa
    12121 gaaagctggg tcttaaagaa agcaccgtaa tgaagatagt aaactatgcc tccagggaca
    12181 actcaagaac ccccgtacag tggtccgcgg agaagaacgc cggcttcacc actgcggaaa
    12241 aggcatggtt ccacattaac cccaactaca ccgaaataaa tgttgaggcc gccgaaaaag
    12301 acccgaattc aatccttaac ttctaccgca agctcataaa gttccgcaag gagaacgaaa
    12361 tcgtcatcta cggctcatac aaggaacatt acaaggacag cgacgagctt tatgtttact
    12421 caaggtacta cgagggcaaa aagatgcttg tcgtatgctc cttcagcgaa aagcctgtaa
    12481 gcttcaaagc tcccgaaggc tttgacctcg ctttgggcga gcttaagctc tcgaactacg
    12541 acaactgcgg catcgccaac aacggctttg tattaaggcc ttacgaaaca agagtttatc
    12601 tgttctcata atgacaatac tatttgcggc tgtataaaat taggaggtat gtgtatgtcg
    12661 gttatatgta aggaagcgta ttacaactcg tcaacaggca aggacactat aagggcgctg
    12721 atttggcatg acgatacagt agcgccgata ggcgtattcc agatagccca cggtgtgtgc
    12781 gagcatattg gcagatacga tgattttgcc cgttttctcg cctccaacgg ctttgtggtc
    12841 tgcggcaacg accatcttgg acacggcaag agcgtaaaat cccttgctga cctcggtcat
    12901 accgccgatg aaaacggcga tgtcagaatg gtagatgata tgcacatcct cacctgcata
    12961 atgaaaaagc gttatcccaa tctgccctat ttccttttcg gacacagcat gggctccttt
    13021 gcggcaagaa tctacgccgc cgatttcggt catgagcttg acggcgtaat cttctgcggc
    13081 actggtcaga ttcccggcgt ggcagctctc ttaaaagacg ccgttgaaaa gactatagac
    13141 aaaatcggag taagaaagag cagcctcggc accatgcagc ttctgg
//
LOCUS       DAONVQ010000045        12949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3693_length_12949_cov_4.847759, whole genome shotgun sequence.
ACCESSION   DAONVQ010000045 DAONVQ010000000
VERSION     DAONVQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12949
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3693_length_12949_cov_4.847759"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLD98_04265"
     CDS             <1..552
                     /locus_tag="PLD98_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQA05498.1"
                     /translation="SASPFEILSDLKTIEMSGLKIQSTGSIMELDTNAELSLSSSKAG
                     ECKAAIVPADYRELSGLYDIIFESQAKSPEHFRIWYAHASHMIRHGFIRAAAVKNKDG
                     TPVACAFTQCENSVLSVITSVAVKEKHRRQGLGSVAVGFLANKLRKEGKKVYLFSKDE
                     NTDKFYLNNGFFVCGNWAKAVIQ"
     gene            582..1856
                     /locus_tag="PLD98_04270"
     CDS             582..1856
                     /locus_tag="PLD98_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HQA05499.1"
                     /translation="MAENYFNIDEKLLELSEKALNRCKEEFIKIDKTTEINQQKVLYA
                     FSANRVSEADLSITTGYGYGDRGREALDKVAAMCLGAEDSIVRHSFASGTHTLTVALF
                     GLLRPGEKMLCVTGTPYDTIRPVIGIKGKEGRGSLKDFGITYMQTELTDSGRVDLDAV
                     EKMLKTEKIKMVYIQRSRGYTLRPSLSVEEIGEIVALTRNFSDAIVMVDNCYGEFVQT
                     KEPTDFGADIIAGSLIKNPGGGIAKCGGYIAGKKELVELCAYRMTAPGIGKEVGATLG
                     HTRDMLMGLFHAPHVVGETLKTAVFCASLYELLGFDASPAHNEERYDIIQSISLGNPE
                     RLIAFCRGLQKGSPIDSFVVPEPWDMPGYDNKVIMAAGAFTSGSSIELSADAPLREPF
                     AVWLQGGLNFHSAKIGILLSAQEMLKEGLISL"
     gene            1909..3375
                     /locus_tag="PLD98_04275"
     CDS             1909..3375
                     /locus_tag="PLD98_04275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA05500.1"
                     /translation="MEEKLKLDYKKTILIGFGFMATSIVWQIYDPYITKILNEMLSNS
                     ELIARWSATLAERYPILLEFLKAQGEKVDTAGGFTLVPLFIGIIMTFDNIFGVIFQPA
                     LGKLSDRTHTRLGKRRPYILVGAPISALLFALIPWMFVKFNSLPGLMICIILFVFVMS
                     TWRAPVVALMPDLTPPELRSEGNAVINLCGGVGGFIGMISGTILSIIFGLIYGKDNFT
                     EQQTYPYVFLLGSVVMIICTLVLFFFVKEPDSRIKAKADANMALEEEKKLKEEKKKKL
                     KEMKLSKAERKSLIFMLFGLFFLFCSTNAITTFFALFAAEILGKTTAQATLMMAVFLV
                     CSMLMAIPAGKLGQKYGRKKTILAGLFIFMGIFLVYLVSYLITGKNWAIWLALVFGGA
                     ANMMITVNTLPLVLEIGGVERIGTFTGYYYTATFSAQIAAPIIYGAVRMFTGTYLSLF
                     VYCPIAFLLAILCISMVKHGEAIPAEELKKIQAEVDAD"
     gene            3487..5097
                     /locus_tag="PLD98_04280"
     CDS             3487..5097
                     /locus_tag="PLD98_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05501.1"
                     /translation="MVSSDFENKDRNTEDFEKNENPIDNNPPIDDREEKSDTPDEETL
                     FQGASLPENTPAENIDDDTRKELEDLARLFQEEYNKTLKEAEEKGEESEEDGSEEETL
                     EERSETESVLNLENLCASCGVRERDLSCVEDYEYCSKCRNDMINRPVPLVSRLFKVLA
                     VFGAVISFFLFVLSFPAIEELAEGDIAIKERRINSAINHYSSAINKFESLSSDDGLAK
                     GYVFEKYIPKASFVKARLIDALYKNGYVTYVESYVDTYFEDRLNNPLLYRVKKLNDEA
                     AVLSHNHQTAYDIIYDDVSKRPNNLPYDDLAKKLDEMKNTPVEGESKKGETYRKVASN
                     LYKYYIASLSGRDFSEQIAMLEEIKELMPEAYWLYGQNLASIYAKAGEKDKALKSVEE
                     LKSYNSEDPMPYAAEAAAHRVSKDFDAAIAAAEKGYNLDPDYGYECLRQKAIAQLLKG
                     QYNEAYETMSFVYEAFLDMDSEDYFIGDMGDTYAITCLAAGKKEEYDSVVELFESYGY
                     NQRPILADYAAGKLTIEQIFLEGKADVA"
     gene            5094..5612
                     /locus_tag="PLD98_04285"
     CDS             5094..5612
                     /locus_tag="PLD98_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05502.1"
                     /translation="MIYLYAILKYLTFPGSIIRATIEHTNCRKFGIPIEDDNPLRFDD
                     LCGHVEHELPKDLTAAARIVLRPFIFNLVVGFFILFYAAVAVFMAGDFSLVDLFLLWL
                     GISLWSNVFPSLEDGSNYLYLARTQGKGISKAIAGFMRVGAALDTWCLSLPLALVFAY
                     ILPIIFKYLFVA"
     gene            5849..6391
                     /locus_tag="PLD98_04290"
     CDS             5849..6391
                     /locus_tag="PLD98_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05503.1"
                     /translation="MKKLIALVLSLVLMFSLPLGTMAASVQAPVVQKAELTPFCADFD
                     ISTILEMADFDLGSIFSFFEDFDIIGMITSLFGNFDLGSLFGGGDFDFLGIIMDLIGG
                     LFGGGGGGGGGGGDDETTEPTDEYTDPSDEYPTDETEPVTDIPKTGASNGAANGLAVA
                     VAITFIASCAFVATRKNKEE"
     gene            complement(6544..6618)
                     /locus_tag="PLD98_04295"
     tRNA            complement(6544..6618)
                     /locus_tag="PLD98_04295"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6583..6585),aa:Glu,seq:ttc)
     gene            complement(6622..6698)
                     /locus_tag="PLD98_04300"
     tRNA            complement(6622..6698)
                     /locus_tag="PLD98_04300"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6662..6664),aa:Met,seq:cat)
     gene            complement(6886..8133)
                     /gene="spoIVB"
                     /locus_tag="PLD98_04305"
     CDS             complement(6886..8133)
                     /gene="spoIVB"
                     /locus_tag="PLD98_04305"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HQA05504.1"
                     /translation="MRKFFKGLTAAMAVFSAVVFGFIIVGERRIPDEFNIVEGAELNI
                     GMFYSASKTSEGEQKEQNVSYSFANEIKNASDVEIKAFRLFPVKRAKVTTNERRYAVP
                     GGEVIGIRLYTNGVIVVGIDDVMSETGLVNPGKAAGLQEGDIIKSIDGKKITQNSDVA
                     KCFENSGGNPVTLQVTRDGKDITLIFRAVKSATDGKYRGGLWVRDSSAGIGTISFYDP
                     ETGIFGSLGHAICDVDTKTVMPLSGGDILTAHLKGVYKGSPGVTGELCGVFGDKVLGS
                     LRINSDTGLYGVLHEPEKKTPLPVATKNEVQPGEAEIYSTVENGKPEKYKIKIVKVNL
                     NEDGTKKNMTIEITDSRLIAKTGGIVQGMSGSPIIQNGMLVGAVTHVFLNNPVQGFGI
                     FAETMLETADKIKAEIDSLAPAA"
     gene            8712..9440
                     /gene="nadE"
                     /locus_tag="PLD98_04310"
     CDS             8712..9440
                     /gene="nadE"
                     /locus_tag="PLD98_04310"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HQA05505.1"
                     /translation="MRDFALEIEKRVEFIRKVLKDAGASGIVYGNSGGKDSALTGILC
                     KMACDNTLGLIMPCHAKRNFEMDLKDGMEVAEKFNIETKVVDLTPIRTETLKTLEKDF
                     SLTDMAIANIAPRLRMTVLYAVAASRNALVAGTDNQSEMYMGYFTKFGDGAYDFNPIG
                     DLTVTEVYEFLKYLGAPECVIKKAPSAALFDDQTDEGEMGITYKKIDEYLMHGTGSKE
                     DIEIIERYHRISEHKRNLPIVYKP"
     gene            complement(9463..9696)
                     /locus_tag="PLD98_04315"
     CDS             complement(9463..9696)
                     /locus_tag="PLD98_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05506.1"
                     /translation="MNALFLGAITPLPDDVKLYIMYLASALLIFLAAVLILKAKPRRR
                     DSIEQLKEVLKKSKEKYIPLDNEIIIEKENKKS"
     gene            complement(9698..10468)
                     /locus_tag="PLD98_04320"
     CDS             complement(9698..10468)
                     /locus_tag="PLD98_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQA05507.1"
                     /translation="MEYTSIFDSHAHYDDAKFDEDRDELISSLPSKGIIGVINCGADL
                     KSSAQSVKLSEKYDYIYAAVGVHPHEAKDFGDDTLNELRRLAANKKVVAIGEIGLDYY
                     YDFSPREKQIEVFEKQLILAAELNLPVIIHNRDAHEDTLNILKKHRPKGVLHCFSGSA
                     ETAREIVKLGMFIGLGGAVTFKNAKKPLEVAATVPDEFLLIETDAPYMAPVPFRGKRC
                     DSAMIPYTAEKIAEVRNTTAQEILNLTAENAKRLFGID"
     gene            complement(10474..12402)
                     /gene="metG"
                     /locus_tag="PLD98_04325"
     CDS             complement(10474..12402)
                     /gene="metG"
                     /locus_tag="PLD98_04325"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQA05508.1"
                     /translation="MDKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARYKRMRGYDV
                     MFLTGTDEHGQKIEDKAKAAGLEPKAYVDNIVVGIKALWKLMNISYDKFIRTTDDYHV
                     KAVQKIFRKLYENGDIYKGVYEGLYCKPCESFWTETQLKDGKCPDCGGEVQKASEEAY
                     FFRLSKYADKLLKHYEENPEFIEPQSRKNEMINNFIKPGLEDLCVSRTSFKWGVPVDF
                     DEGHVVYVWIDALSNYITALGYGSDDDSLYKKYWPADVHLMAKEIVRFHSIIWPAMLM
                     SLGEPLPKKVFGHGWLLLEGGKMSKSKGNVVDPVVLCERYGVDAIRYFLLREIAFGSD
                     GVFSNENLINRINFELANDLGNLLSRTVAMVEKYFGGTIPAEREKTEFDESLISMAKG
                     LKANTESLYDQLLFPNALTDIWKFISRANKYIDETTPWLLAKDESKKTVLANVLYNLC
                     ESLRIVSVLINPVMPETSPKIRQQLGLTDEIEWDSAGVFGLKDSYSVQKGEIIFPRID
                     TEAELKELEKLMPLAKEEEKKSAENIALIGIEDFAKVNLKVVKVLACEPVKKAKKLLK
                     LTVDDGERQDRTIVSGIALWYKPEDLIGRNVIIVDNLKPAVLCGVESQGMILAADAGE
                     NDVKVIFADDVPVGSKVR"
     gene            12696..>12949
                     /locus_tag="PLD98_04330"
     CDS             12696..>12949
                     /locus_tag="PLD98_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQA05509.1"
                     /translation="MVSAGDFKNGLTFEMDGEVYQVIEFQHVKPGKGAAFVRTKIRNV
                     IAGSVVERTFSPTDKFPTAYIERKEMTYSYSDGDLYYFMDP"
BASE COUNT         3482 a         3033 c         2912 g         3522 t
ORIGIN      
        1 agcgcatccc cctttgaaat cctgtcggac cttaagacaa ttgaaatgtc gggtcttaag
       61 attcaaagca ccggcagcat aatggagctt gacacaaacg cggagttgtc tttgagctcc
      121 tcaaaggccg gggagtgcaa agcggcaatc gtgcccgcag actaccgtga gctgagcggc
      181 ttatatgata ttatttttga atctcaggca aagtcccccg agcatttcag aatctggtac
      241 gcccacgcct cgcacatgat aaggcacggt tttataaggg cagcggcggt aaaaaataaa
      301 gacggcaccc ccgttgcctg cgcctttacc cagtgcgaga acagcgtttt aagcgtaatc
      361 acttccgttg ccgtaaaaga aaaacacagg cggcagggtc tcggcagcgt cgcggtaggc
      421 tttctggcta ataaactgcg taaagaaggc aaaaaagttt atcttttttc caaggatgaa
      481 aatacagata agttttattt gaacaacggc ttttttgtat gtggtaattg ggcaaaagct
      541 gttatacaat agtagagaaa tttaaaatag cggagttata aatggcagag aattatttta
      601 acattgacga aaaacttctt gagctttccg aaaaagctct gaatagatgc aaggaagaat
      661 ttataaaaat agataagacg acagaaataa atcagcagaa ggttctttac gcctttagcg
      721 ccaacagggt cagcgaggcg gatttatcaa tcaccacagg ctacggctac ggcgacaggg
      781 gcagagaagc ccttgacaaa gtcgccgcca tgtgcctcgg agcggaggat tccatagtcc
      841 gccacagctt tgccagcggc acccacaccc tcaccgtcgc ccttttcggt ctcttgcgcc
      901 caggggaaaa aatgctttgc gttacgggca ccccttatga tacaattaga cccgtaatcg
      961 gcataaaggg caaagagggc agaggttccc taaaagactt cggcatcacc tatatgcaga
     1021 ccgagctgac agatagcggc agagtggacc ttgacgccgt tgaaaagatg ctgaaaaccg
     1081 aaaaaattaa gatggtttat atccagcgct caaggggata cacacttcgc cccagccttt
     1141 ccgtggagga aataggtgag attgttgccc ttaccagaaa cttcagcgac gccattgtga
     1201 tggtagacaa ctgctacggc gagtttgtcc agaccaagga gcctaccgat ttcggcgcag
     1261 acataatagc aggctcgctt ataaagaacc ccggcggcgg cattgcgaag tgcggcggct
     1321 acatagccgg caaaaaggag cttgtggagc tgtgcgccta cagaatgacc gcgcccggaa
     1381 tcggcaagga ggtgggcgcg actctcggtc acacaagaga tatgctcatg ggtcttttcc
     1441 atgcgcccca tgtggtcggc gagacgctta aaaccgccgt cttttgcgcg tcactttacg
     1501 aacttttggg atttgacgcc tctcccgcac ataacgagga gagatatgat ataatccaga
     1561 gcataagtct tggaaacccc gaaaggctga ttgccttctg cagaggcttg caaaaaggct
     1621 cgcccataga ttcctttgtc gttcccgagc cgtgggatat gcccggctac gacaacaagg
     1681 taattatggc cgcgggagcc tttacctcgg gctcatccat cgagctctca gccgacgcgc
     1741 ctttaagaga gccctttgcc gtttggctcc agggaggtct taacttccac agcgccaaaa
     1801 tcggcatact tctttccgct caggaaatgc tgaaagaagg ccttatctct ttataatcga
     1861 caccaaccct gtcgggttta ccataacaac ctttaggagc gtgtacttat ggaggaaaag
     1921 ttaaagctag attacaaaaa gaccatacta ataggctttg gattcatggc aacatccatt
     1981 gtatggcaga tttacgaccc gtacataacc aagattctca atgagatgct gtcaaattca
     2041 gagcttattg cccgttggag cgcgaccctt gcggagcgtt atcccattct gcttgagttc
     2101 cttaaagccc agggcgaaaa agtcgacacc gcaggcggct ttacgcttgt ccccttgttc
     2161 atcggaatta taatgacctt tgacaatatc ttcggcgtta tattccagcc tgccctcgga
     2221 aagctttcgg acagaaccca cacaaggctc ggcaaaaggc gcccctacat cttggtcggc
     2281 gcacccattt cggcgctgct gtttgctctt attccctgga tgttcgttaa gttcaacagc
     2341 ctgcccggac ttatgatttg cattatcctc tttgtctttg ttatgtccac atggcgcgcg
     2401 ccggttgtcg ccctaatgcc cgaccttaca ccgcctgagc tcagaagtga aggcaatgct
     2461 gttatcaacc tctgcggcgg tgtcggcgga ttcatcggca tgatttcggg tacaattttg
     2521 tcaattatct ttggtcttat ctatggcaag gacaatttca cagagcagca gacttatcct
     2581 tatgtattcc ttttgggctc tgtcgtaatg ataatctgca cccttgtcct gttcttcttc
     2641 gtaaaggagc ctgacagccg aatcaaggca aaagccgatg cgaacatggc tttggaagag
     2701 gagaagaagc ttaaagaaga aaagaaaaag aagctcaagg aaatgaagct ctccaaggca
     2761 gagagaaaga gcctcatttt catgctgttc ggacttttct tcctgttctg ttccacaaac
     2821 gccataacaa ccttctttgc gctgtttgcg gctgagattc tcggaaagac aacagctcag
     2881 gcaaccttaa tgatggctgt gttccttgtc tgctcaatgc ttatggcaat cccggcagga
     2941 aagctcggtc agaaatacgg cagaaaaaag actatccttg caggactctt tatcttcatg
     3001 ggaatcttcc ttgtatatct tgtttcttac cttatcacag gcaagaactg ggctatctgg
     3061 ctggcactcg tattcggcgg agcagctaac atgatgataa ccgtcaatac gctgcctctc
     3121 gttctcgaaa tcggcggtgt cgagagaata ggcaccttca ccggctacta ctacacagcc
     3181 acattctccg cccagattgc ggctcccatc atctacggcg cagtcagaat gttcacgggc
     3241 acatatctct cactctttgt atactgcccc atcgcgttcc ttctcgcaat cctctgcatc
     3301 agcatggtta agcacggcga ggcaattccc gccgaggagc ttaaaaagat tcaggctgag
     3361 gtagacgctg actaaaaaat taatttctat tgatttgtgg aaattaactg tttaaaataa
     3421 taatgccttg cctttgcacc agcaaaaagc aaaggcaggg ctattaaaac tttaggagga
     3481 aaagttttgg tttcatccga ttttgaaaac aaggatagga ataccgagga ttttgaaaag
     3541 aacgaaaatc ctatagacaa taaccctccc atagatgaca gggaagaaaa atccgatact
     3601 cccgacgaag aaactttgtt tcagggggct tctttgcccg agaatacacc tgcggaaaat
     3661 attgatgacg acacaagaaa agagctggag gacctcgcaa ggctctttca ggaggaatac
     3721 aacaagacct taaaagaggc ggaggaaaag ggagaagaaa gcgaagaaga cggctcagag
     3781 gaagaaacac tcgaggagcg gagcgaaacc gagtctgtgc tgaatctcga aaacctttgc
     3841 gcaagctgcg gcgtgcgtga aagggattta agctgcgtcg aggattatga gtactgctca
     3901 aagtgcagaa atgacatgat taaccgcccc gttcctcttg tttcacggct gtttaaggtt
     3961 ctcgctgttt tcggcgcggt catcagcttt ttcctctttg ttctgagctt tcccgccatt
     4021 gaggagcttg ccgaaggcga catcgccata aaagaacgaa ggataaactc cgccatcaac
     4081 cactattcct ccgcgataaa caaattcgaa agccttagct ccgacgacgg tttggcaaag
     4141 ggctatgttt ttgaaaagta tattcccaag gcaagctttg tcaaggcaag gcttattgac
     4201 gctctttaca aaaacggata tgtaacctat gtggagtcct atgtggatac ctactttgaa
     4261 gacaggctta ataatccgtt gctatacagg gtaaagaagc tgaatgacga agccgcggtt
     4321 ctttctcaca accaccagac ggcatacgat ataatttatg acgatgtctc aaaacgcccc
     4381 aacaatcttc cctatgacga ccttgccaaa aaacttgacg aaatgaaaaa caccccggta
     4441 gagggcgaga gcaaaaaagg cgaaacttac agaaaggtag cctcaaacct ctacaagtac
     4501 tatatcgcgt cactcagcgg ccgtgatttt tccgagcaga ttgccatgct tgaggagatt
     4561 aaggagctta tgccggaggc atattggctc tacggtcaaa accttgcctc aatttacgca
     4621 aaggccggag aaaaagacaa ggctttaaag agcgtagagg agcttaaaag ctataactcc
     4681 gaggacccga tgccctatgc cgccgaggct gccgctcaca gagtttctaa agactttgac
     4741 gccgccatcg ccgccgcgga aaagggatat aaccttgacc ccgactacgg ctatgagtgt
     4801 ttaaggcaaa aggcaatagc ccagctctta aagggtcagt ataacgaggc ttacgaaacc
     4861 atgagctttg tttacgaagc cttcctggac atggattccg aagactactt catcggcgac
     4921 atgggcgaca cctatgccat cacctgcctt gccgcaggaa agaaggaaga atacgacagc
     4981 gtagttgagc tgtttgagtc ctacggctac aatcaaaggc ccattctggc ggactacgcg
     5041 gcaggaaagc tcaccatcga gcagatattc cttgaaggaa aggcggatgt ggcatgattt
     5101 atctttacgc aatattaaaa tatctcactt tccccggcag catcataaga gcaaccatag
     5161 agcatacaaa ttgcagaaag tttggaatac ccatagagga cgacaatcct ttaaggtttg
     5221 acgacctttg cggtcatgtg gagcatgaac tgccgaaaga ccttacggcg gccgcgagaa
     5281 tcgtgctaag acccttcata ttcaatttgg ttgtaggctt ctttattctg ttttacgccg
     5341 cagtcgcggt ctttatggca ggcgatttca gccttgtgga tttattccta ctgtggctcg
     5401 gaatttccct gtggtccaat gtgttcccct cccttgagga cggctcgaac tacctctatc
     5461 tcgcaagaac tcagggcaag ggaatttcca aggctatagc gggctttatg cgcgtgggag
     5521 ctgctttgga cacatggtgt ctcagcctgc ctttggctct cgtttttgcg tatatcctgc
     5581 caataatttt taaatatctt tttgtggcgt aacactaagg atttcaaagt tttatgttgt
     5641 tttgtatctg ttaatagatt taacacattt ccgcaataat tttcagtctt gtttttagat
     5701 tttatataaa aatgagatgt gtaaatatac catttgaaca aaattatgtt cgaataaaga
     5761 ataacattga caattcacca aaaaaaagtt attctaaatt acagggtatt agcataattc
     5821 tgatttcata ttgaaaggat tgtttcggat gaaaaagcta atcgcacttg tcctctcgct
     5881 tgtattgatg ttttctcttc cgctcggcac aatggcagca agtgttcagg ctcctgttgt
     5941 tcaaaaagcg gagctcacac ctttctgtgc agactttgat atctcaacaa ttctggaaat
     6001 ggccgatttt gatttaggct cgattttcag cttttttgaa gactttgaca taatcggaat
     6061 gattacaagt ctcttcggca actttgacct cggcagtctg tttggcggcg gagacttcga
     6121 cttcctcggc atcatcatgg acctcatcgg cggcctcttc ggaggcggcg gtggcggcgg
     6181 cggtggcggc ggtgacgacg agaccaccga gcctaccgac gagtacaccg atcccagcga
     6241 tgagtatccg acagatgaaa ccgagcctgt aaccgacata cccaagacag gtgcgtcaaa
     6301 cggcgcggca aacggccttg cagtagctgt tgctattaca ttcatagctt cctgcgcctt
     6361 cgtagcaaca aggaagaata aagaagaata atcacagctt ttccgaaaac ttttcaatat
     6421 ttagataaac ccggctgttg ctattaagca gcagcctttt tgtttttatt ggaaaacaaa
     6481 aaaagcggca aaaaccgcct taaacaaaaa ataaaaccga gcacctataa gatgctcggt
     6541 ttgtggtgac ccggacgaga ttcgaactcg tgttaccgcc gtgaaagggc ggtgtcttaa
     6601 ccccttgacc accgggccgc atggtagcgg cagtcggatt cgaaccaacg acacttcggg
     6661 tatgaaccga atgctctagc caactgagct atgccgccat atcgcgctgt ggaacagcga
     6721 acacaattat aatataagct gagatacttg tcaactcttt ttttaaatat aaattttata
     6781 agcgtaattt ttttggcgtc aatgtattta ttataagccg attattattg tgcgttaaaa
     6841 aaataaaaat atacggcctg catttgcaag ccgttttttt gtgttttaag ccgcaggcgc
     6901 gaggctgtcg atttccgctt taatcttgtc agccgtttca agcatcgtct ccgcaaagat
     6961 tccaaagccc tgaacgggat tgtttaagaa cacatgggtt acggcgccga caagcatacc
     7021 gttttgaatt atcggactgc cgctcatgcc ctgaacaata ccgcccgttt tcgcgatgag
     7081 tcttgagtcg gtaatctcaa ttgtcatatt ttttttcgtg ccgtcctcgt tgagatttac
     7141 ctttactatc ttaatcttgt atttttcggg ctttccgttt tcaactgtgg agtaaatctc
     7201 cgcctcaccg ggctgaacct cgttctttgt cgctacaggc aggggagttt tcttttcggg
     7261 ctcatgcaga acaccgtaaa gtcccgtatc ggagtttatt ctaagtgagc cgagaacctt
     7321 gtcgccgaat actccgcaaa gctcgcccgt tactccggga ctgcctttat atacgccctt
     7381 tagatgagcc gtgagaatgt caccgccgct gaggggcatt acggttttgg tgtccacatc
     7441 gcatatagcg tgtcccaaag agccgaatat gcctgtttcg gggtcgtaga aggagatagt
     7501 gcctattccc gccgagctgt ctcttaccca cagaccgcct ctgtatttgc cgtcagtggc
     7561 tgatttaaca gctctgaata ttaaggtaat atccttaccg tctctcgtca cttgaagcgt
     7621 tacggggttt ccgccgctgt tttcaaagca ttttgccaca tcggagttct gagtgatttt
     7681 ctttccgtca atgctcttta taatgtcccc ttcctgaagt cccgcagcct ttccgggatt
     7741 tacaagtcct gtctcgctca tcacatcgtc gattccgacg acaattacac cgtttgtgta
     7801 aagtcttatt cctataacct ctccgccggg gaccgcgtat ctgcgctcat ttgtggttac
     7861 cttcgctctt tttacgggga acagcctgaa ggcttttatc tccacatcgc tggcgttttt
     7921 aatttcattc gcaaagctgt agcttacatt ctgctccttc tgttctccct cggaagtttt
     7981 gcttgccgaa taaaacattc cgatatttaa ttcggcgccc tctactatat tgaattcgtc
     8041 aggaattctg cgttcaccga caataataaa gccgaaaacc actgccgaaa aaacggccat
     8101 ggcggctgtt aagcctttga aaaatttccg catattatga ccaacctttc actttgcaga
     8161 aagcttttgt gaccgcatat ttaaggctga atatcgccta acatcttgat ttttttgcga
     8221 ttcaaaacaa aattactttc cgctcactct tataatttgc ataagcggct ttttaatttg
     8281 cggcaattct tttcgctgtc ggtgaattct tgaataaaag agatagaaat tgcctcgtat
     8341 attttttgaa aataggcaag acttttaaaa tcaaggcata aaaaaagctg ccgaatatat
     8401 cctgacgaac tttattttca taatcttcaa actgttctat aattttttta attaggttta
     8461 gatttttaag aataaataaa aatctcttta tttatgttgc tttctctttt ttgagaattt
     8521 ttactgcctt tattattccc agaaaaggca ctgtcaaatc aaactttgag acaacttgca
     8581 aaaaatagca tcataaaaat tgacaattgt ttttttctta atctataata gtttaaaagc
     8641 tttaagcgtt taactcagca ctgtcttaat agcagactaa atttaatttt tatatatgag
     8701 agggaaatgc tatgagggat tttgctttgg aaattgagaa aagagtggag tttatcagaa
     8761 aggttttgaa agacgccggc gcgagcggaa tagtttacgg aaacagcggc ggcaaagaca
     8821 gtgccctgac aggtattctt tgcaaaatgg cgtgtgataa tacgctgggt ctcatcatgc
     8881 cctgccacgc gaaaagaaat tttgaaatgg accttaaaga cggcatggag gttgccgaaa
     8941 aattcaatat cgagacaaag gtagtggacc tgactcccat aagaacggaa acgcttaaaa
     9001 ccctcgaaaa agatttttcg cttaccgaca tggcaatagc taacatagct cccagactca
     9061 gaatgacggt gctctacgct gttgccgcaa gcagaaacgc ccttgtcgcc ggtaccgaca
     9121 atcagagcga aatgtatatg ggctacttta caaagttcgg cgacggagcc tatgacttta
     9181 accccatcgg cgaccttacc gtaaccgaag tctatgagtt ccttaaatat ctcggcgctc
     9241 ccgagtgcgt aattaaaaaa gctccctccg cagccttgtt tgatgaccag accgacgagg
     9301 gagaaatggg gatcacatat aaaaagattg acgagtatct tatgcacggc accggctcaa
     9361 aggaagatat tgaaatcatc gagcgctacc acagaattag cgagcataag agaaacctgc
     9421 ccatagttta taaaccgtaa aaataagagc taaggcaaaa ccttagctct ttttgttttc
     9481 tttttctata attatttcat tatccaaagg tatgtatttt tcttttgatt tttttaaaac
     9541 ctcttttagc tgctcaatgc tgtcccttct gcggggcttt gctttaagaa ttaacaccgc
     9601 cgcaagaaaa atcagcaaag cagaggccaa atacatgata taaagcttta catcatcggg
     9661 caggggagtg attgccccca aaaacagagc gttcatatca gtcaatccca aatagccttt
     9721 ttgcgttttc ggctgtgagg ttcaaaattt cctgcgctgt cgtgtttctg acctcggcta
     9781 ttttttcagc cgtatagggt atcatcgcag agtcgcatct cttgcccctg aacggaacgg
     9841 gggccatata gggagcgtcg gtttcgataa gcaaaaactc atcgggcact gttgccgcta
     9901 cctcaagggg cttttttgcg tttttaaatg taaccgcgcc gccgagacct atgaacattc
     9961 cgagtttaac aatctccctt gccgtttctg cgctgccgga aaagcagtgc agaacgccct
    10021 tcggacggtg ctttttaagg atattcagtg tatcttcatg ggcgtctctg ttgtgaatta
    10081 tcacggggag attaagctcc gcggcgagaa taagctgctt ttcaaaaact tcaatctgct
    10141 tttcccgcgg cgaaaaatca tagtaatagt caagacctat ttcgccaatc gccacaacct
    10201 ttttgtttgc ggccagccgc cttaattcat taagcgtatc gtcgccgaaa tccttagcct
    10261 catggggatg aactcccaca gcggcgtaaa tatagtcata tttttcgcta agtttaacgg
    10321 actgggcaga cgactttaaa tccgcgccgc agttaattac cccaataatg ccttttgagg
    10381 gcagggacga aataagttca tccctgtcct catcgaattt ggcgtcatca taatgcgcgt
    10441 gggaatcaaa aatcgaagta tactccataa aaactatctg acctttgaac cgacgggaac
    10501 atcgtcggcg aagattacct taacatcgtt ttcgcctgcg tcagcggcaa gtatcatgcc
    10561 ttggctttca acgccgcaga gaacggcggg ctttaagtta tcgacaataa taacattcct
    10621 gccgattaaa tcttctggct tataccaaag agcgataccc gatacaatcg ttctgtcctg
    10681 tctttcgccg tcgtcaacgg tgagtttaag gagcttcttt gccttcttta caggctcgca
    10741 ggcaaggacc tttacaacct ttaagtttac cttagcgaag tcctcaatgc cgatgagagc
    10801 tatattctcc gcactctttt tctcctcttc ctttgcaaga ggcatcagct tttcaagctc
    10861 tttaagctca gcctctgtgt cgattctcgg gaagatgatt tcacccttct gaacgctata
    10921 gctgtcttta agaccgaata ctcccgcgct gtcccattcg atttcatcgg taagaccgag
    10981 ctgctgtctg attttcggtg aagtctcggg cattacggga tttataagaa cggaaacgat
    11041 tctcaagctc tcgcaaaggt tgtagagaac attcgccagc actgtctttt tgctttcgtc
    11101 ctttgcaagg agccacggag tggtttcgtc aatatatttg tttgctctgg aaataaactt
    11161 ccaaatatcg gtcagagcgt tggggaacaa aagctggtcg taaaggctct ctgtatttgc
    11221 cttaagtccc tttgccatgc tgattaagga ctcgtcaaac tcggttttct ctcgctcagc
    11281 ggggatagtt ccgccgaaat acttttcaac catggcgact gtgcgtgaaa gcaggttgcc
    11341 caggtcgttc gcaagctcga aattaatgcg gttgataaga ttctcatttg agaaaacgcc
    11401 gtcggagccg aaggcaatct ctcttaaaag gaagtatctt atagcgtcaa cgccgtatct
    11461 ctcgcagagc acaacagggt caaccacatt gcccttggat ttgctcatct tgccgccctc
    11521 aagcagcaac cagccgtgac cgaaaacctt tttgggcagc ggctcgccga gggacatcag
    11581 cattgcgggc caaataatcg aatggaaacg gactatctcc tttgccataa gatgaacatc
    11641 ggcgggccag tattttttgt aaaggctgtc gtcgtcggag ccgtagccca gagcggtgat
    11701 gtagtttgaa agagcgtcta tccaaacata aaccacatga ccctcatcaa aatcgacggg
    11761 tacgccccac ttgaaggagg ttctcgaaac gcaaaggtcc tcaagtccgg gcttaatgaa
    11821 gttatttatc atttcatttt ttcttgactg aggctcgata aactcgggat tttcctcata
    11881 gtgcttcagc agcttgtcgg cgtacttgga gagcctgaag aaataagcct cctcggatgc
    11941 cttctgaacc tcgccgccgc agtcggggca tttgccgtct ttaagctgag tttcggtcca
    12001 gaatgactca cagggcttgc agtaaagtcc ctcgtaaacg cccttgtaaa tgtcgccgtt
    12061 ttcgtaaagc tttctgaaaa tcttctgaac ggcctttaca tggtagtcgt cggttgtcct
    12121 gatgaatttg tcgtagctta tgttcatcag cttccacaga gctttgatgc ctacaactat
    12181 attgtcaaca taggccttag gctcaagacc tgcggcctta gccttgtcct caatcttctg
    12241 tccgtgctca tccgtgcctg taaggaacat tacatcataa ccgcgcattc gcttatacct
    12301 ggccatagcg tcagcggcaa ccgttgtgta gctgtggccg atgtgcagct tgtccgacgg
    12361 gtaataaatc ggggttgtga tgtaaaatgt ctttttgtcc atatcaatgc tccttttact
    12421 ttgcattcgg cacttaaaat ataagccgaa aaatttctaa aatttactaa atcgataaaa
    12481 ctattataac acttatatcg atttaatcaa tatttctttg gcaggtatgg agtttaactt
    12541 cggggagatt ttaataatga aatagatttt actgttagta aaatcggctt ttttggcatt
    12601 tttattcaag ccaatctata tttattaaaa tctattgaaa gtttggaaca aataggatat
    12661 tattatatag cattcaaatt tccggaggaa tatatatggt ttcagcaggt gattttaaaa
    12721 acggtcttac ctttgagatg gacggagaag tgtatcaggt tatcgagttc cagcatgtaa
    12781 agccgggtaa aggcgccgct tttgtaagaa caaagataag gaatgttatc gcaggttccg
    12841 ttgttgagcg caccttcagc ccgacagaca agttcccgac cgcatacatc gagcgcaagg
    12901 aaatgaccta ttcctacagc gacggagatc tctattactt catggaccc
//
LOCUS       DAONVQ010000046        12910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3705_length_12910_cov_4.279347, whole genome shotgun sequence.
ACCESSION   DAONVQ010000046 DAONVQ010000000
VERSION     DAONVQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3705_length_12910_cov_4.279347"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="PLD98_04335"
     CDS             <1..1275
                     /locus_tag="PLD98_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQA05510.1"
                     /translation="IMVNSGFLNGLSVEEAKEKIIEWLIKEGKGERKTNYKLRDWVFS
                     RQRYWGEPIPIVECPKCGYVPLPESELPLELPKVESYEPTDTGESPLAKLTDWVNTTC
                     PKCGGAAKRETDTMPQWAGSSWYFLRYCDPHNDECLASKEALDYWTPVDWYNGGMEHT
                     TLHLLYSRFWHKFLYDIGVVPTKEPYKKRTSHGMILGENGEKMSKSRGNVVNPDEIVN
                     TYGADTMRLYEMFIGDFEKAAPWSSTSIKGCKRFVDRYWSFRDILIPDKKIRDELLSS
                     FHKTIKKVTHDIDNLKMNTAIAALMSLMNEISETGKITREELRIFTILLNPFAPHVTE
                     EVWELEGFGGRVTDAKWPEYSEELCEEKKVEIAVQINGKVRSRIVIDADATQEQAIAD
                     AKADARIREELSGKTVLKEIYVKGKLVNIVAK"
     gene            complement(1344..3278)
                     /locus_tag="PLD98_04340"
     CDS             complement(1344..3278)
                     /locus_tag="PLD98_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05511.1"
                     /translation="MSTRAPMRAPMRGPGRGAPGLKPVNTKKTVGRLIKYITKNKLIL
                     VLVAFLVLIASGAGVAGTYMLTPVINTIEKAFKGEITDPVAKILGYIAILAVIYIAGA
                     AAQYAYQRVMVGLTHRTLNAIRRDLFDHLQDLPIDFFDKNTHGEIMSRFTNDTDTLRE
                     ALSQGLTQILTSLVSVTGTFIMMVILSPALMILILIMLAIMLFAIRTIGSRSAKYFRL
                     QQRTVGAVNGYIEEMIEGQRVVKAFCREDKVIEDFGKLNNDLKSASVNAHTFASVIMP
                     VMGNLSYMNYALTATIGAYLIIKKAATGVIGLSIGQLASFLQYTRQFSMPIANVSQQV
                     NNILAALAGAERIFEIIDYPAETDDGVVTLVNAKVNDDGSIEEVDYRTGVWAWKQPND
                     DGSVSYTLVKGDVRFKNVSFAYDKDKPVLKNINIEGAAGHKFAFVGSTGAGKTTITNL
                     INRFYDIQEGEITYDGIDIKKIKKADLRRSLGMVLQDTHLFTGTVEDNIRYGRLDATF
                     EDIVNAAKLANAHSFIERLPQGYQTMLTSDGGNLSQGQRQLISIARAAVANPPVLILD
                     EATSSIDTRTEHLVEKGMDSLMEGRTVFVIAHRLSTVRNSNQIIVLENGEIIEQGDHN
                     TLIEKKGRYYALYTGQFELS"
     gene            complement(3275..5077)
                     /locus_tag="PLD98_04345"
     CDS             complement(3275..5077)
                     /locus_tag="PLD98_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05512.1"
                     /translation="MNLKKLTPFMKNQKLYAILTPLTVMLEAMLEVCIPALMSVIVDS
                     GLYGKDLSETADLFLIRTLINMGIISGSGTQLIYRTGTVMVALSIVSLCLGILGARFA
                     AVAGMGFGADLRMGLFKKVQNFSFDNIDSINTASLITRMTTDINLIQMTFMMIIRTCA
                     RAPFMFLLAIIYAIKINARLSIIFLVIAPILGFMLVFIILKAFPRFRAMFEKYDRFNA
                     AIQENLIGIRVVKTFVRAEHEKEKFRISNDELRDASIFAEKLAILQNPILQLSMYTAI
                     TLILWFGGNMVLSGSMGTGELMSFIAYVHQILFSLMMVSMVFIMIVISRASIQRAIEV
                     LSTEPIIKDDDADENLSVQDGSIVFDNVSFKYSKSAKKNVLDNINLSIKSGEVIGIIG
                     GTGSSKSTLVQLIPRFYDVTEGAVYVGGRNVKDYKLRKLRDAVAMVLQKNLLFSGTIE
                     ENLRWGNENATMDEIITACKAAAAHDFIMSFPDGYQTMLGQGGTNVSGGQKQRLCIAR
                     TLLKKPKILILDDSTSAVDTKTDALIRESLKNYFPETTKIIIAQRVNSVKHADRIIVL
                     DEGRISEIGTHEELLKLGGIYAEVCRSQQEDSEI"
     gene            complement(5117..5551)
                     /locus_tag="PLD98_04350"
     CDS             complement(5117..5551)
                     /locus_tag="PLD98_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQA05513.1"
                     /translation="MSPKELITADKFIVTHILFRKRLHQRFKERDIYPGQPAILHIIN
                     EFGVCSQKDICDKIMTSPASVAVSIRRMQNMGLIEKLTDEKDMRFNKIRLTEKGVEAH
                     NYCRNELKALSDEMLDGFSENEKNEFDRMLVKMIENLSKEEQ"
     gene            5968..6867
                     /locus_tag="PLD98_04355"
     CDS             5968..6867
                     /locus_tag="PLD98_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05514.1"
                     /translation="MIEAVNVTKKFGDFTAIESLSLTVPKGSIYGLVGYNGAGKTTLL
                     KTIAGVYRPEGGKVLIDGEPVFENEKKKQRLFYVPDEIYFKPYATMNKMAAFYKGYYP
                     NFSYDTFEKVTKLFGLDPTKRLNGFSKGMQRQAEVVFAMSTMPETLLLDESFDGLDPQ
                     KRAVVKELLYDYMSRKDVATIISSHNLHELQDLCDYVGLINGKRIVLSGSVSDLSKGK
                     AKYRLVFDRPVTEQDFKDIPHKRLNIDGSIVTLMALGTPEEAYAKLKAMSPLLIEETL
                     LTLEEIFLDEMEAEGNEAKKIFG"
     gene            6842..9337
                     /locus_tag="PLD98_04360"
     CDS             6842..9337
                     /locus_tag="PLD98_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05515.1"
                     /translation="MKQKRSSANPFTHTFSESLKSTNIVVTLLMLAVMAFMFFSMLSY
                     PLSYRDPYMESPTAKDQYIYYLFAPNELATVFACGLMLVAGILMGLSSFRFITSKKTV
                     NVYYSLGIKREKLFLAHYLSGALLLVVGAVLSVMVGVVMNLIYIGSSFELWQAATYYM
                     MAFATIALFSYSVTAAVLSSVGTVFEGGIFSVIISFLPTFLLSSVQFLMNKLVWGNPF
                     GVAFVETGLTHQYNSPSLVTHFSYLTPLTAFSSTLVEIADREKPLNADQAEKAAETIY
                     ELAASDYLTDLFWLIAIAAIFFLGMYLFKRRRAEISGFIGTNRVLNNVGIFVVAIFTF
                     SVIFTSSPLQKYAAFGIALFAALLVFVILDAVLRRSFKEFKRDLVFLPMHLAVITAIF
                     FIFSTGLFGYSKRVPAAEDIKSVSVSTVGVQSFFSEYYPMREDILVYPGSNEMISGFE
                     SEKDIKLIRDIHAKIAELNAADAYEMWKFSADHSSKRLASRIQFTYYLKNGKTVRRSF
                     RNVSAGVIEELLKIYDTERFKKAADEALSPDTPIMQNERARLLRSEGVIVRVYPKDLS
                     YIARLNLTSEQRNELYEALRQDIAEQTYKERYFPEKPPLGVISIVGNNYTDFDKGIIE
                     VIEDENGRAYEKAIEPPSKADDIIKPPSGEPWDILDNSPTIAMHGTTDFNIDFVITED
                     MERTIKFLKDNNMIDAFKGSEKKYTSVQLVSIERSFTFQPFMLKYGFDFHYDAFGKSI
                     SKAKSNEEYRYDPLENQKTYTVTDAETIKVLKDISYLTYFSSGTGYAAVFNAEDGTAQ
                     YLYIPASKMPAALAGAVRNFDTEQLRDFNLSNY"
     gene            9452..9880
                     /locus_tag="PLD98_04365"
     CDS             9452..9880
                     /locus_tag="PLD98_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQA05516.1"
                     /translation="MEKKKAFTLSEKVLVGVIIALAFLLVAQRISAYRYFSRLDAIEI
                     IASEEKTAAETASETKSEETKSETETESEAEPEKRLININTASKEELILLKGIGEKKA
                     QAIIDYRNENGGFDKIEEIMNVSGIGEKTFLNIKSYITVD"
     gene            10011..10595
                     /gene="yfbR"
                     /locus_tag="PLD98_04370"
     CDS             10011..10595
                     /gene="yfbR"
                     /locus_tag="PLD98_04370"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HQA05517.1"
                     /translation="MSGSHFFAFFARMKYINRWSLMRNVFYETLSQHSTEVAAIAHML
                     ALIGNKRFGKSYNAERAAVLGLYHDMPEILTGDMPTPVKYFNSEIKNAFKSVEREAAA
                     RLTDSLPGYLRDEFKTIFSESRDDAEEWRLVKAADKIAALIKCIEEQKAGNTEFDKAK
                     QSVENTLKEMECPEAEVFIEEFLPSFSLTLDQLK"
     gene            10617..11639
                     /locus_tag="PLD98_04375"
     CDS             10617..11639
                     /locus_tag="PLD98_04375"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQA05518.1"
                     /translation="MDIKNILEKLISSKGVSGNEALAANTAAEILADYMPTEIQSSGN
                     VVGFLDGEGPIIMLEAHLDQIGLIVTDIDKSGFIRFAKCGGADMRVLPAADVTIVGKD
                     GVEIDGIVISTPPHLAKDEDKKKFKQIEDLAIDVGLCYEDALKVIRPGDRVTYKKAFG
                     SLMNNRLYGTSFDDRAGVAAVLKAVDLLKGQKLNCKLCVLFAALEETTGGGAAVGAFA
                     HQPDESISVDVSFAESPGVPEHKTGKLGGGAMIGIAPTLDDEISGKLIKIARTKKIKY
                     QTEVMGGQTSTNADKISVAGRGVKTGLISIPLRNMHSAVEVLQLDDVEAVAQLLAEYV
                     LSKGVM"
     gene            11639..12655
                     /locus_tag="PLD98_04380"
     CDS             11639..12655
                     /locus_tag="PLD98_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQA05519.1"
                     /translation="MLELLKQMAEITAVSGKEGVLRDLIIEQIKPFAEYEIDPLGNLL
                     VRKKGRRKAKNKVMLAAHMDEVGIIVTYAEKSGFLRFATVGGIDPKVLIGREVVFENG
                     TRGVTGLSPVHLLKDSEKDAVPEISSLYIDIGAENDEQALSAVPLGTRGTFREKFEYF
                     GDGLIAGRALDDRIGVALMVDIIRSEIEYDLHFAFTVQEEVGARGAAAAAFSVAPDYA
                     IVLEATTAADIIDVPEHRTVCALKNGPAVSFMDHSTVYPMELYDLAMKVAKDNGIKAQ
                     PKAAVAGGNDAGSIHKSGKGVKAITLSVPCRYIHSQNCVAAVQDMESARALAEKLAAE
                     LANA"
     gene            12648..>12910
                     /locus_tag="PLD98_04385"
     CDS             12648..>12910
                     /locus_tag="PLD98_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05520.1"
                     /translation="MLKLLNDDTYFQARRQLENNQFGAVIGSLYKAYGNSCDYCVCLC
                     QKSDDGRITALISVFMGSMTLYCETDADFEELRLYIKSASPFE"
BASE COUNT         3474 a         3157 c         3102 g         3177 t
ORIGIN      
        1 attatggtaa actccggctt cctcaacggt ctctccgtag aggaggctaa ggagaagatt
       61 atagagtggc tcattaaaga gggcaagggc gagagaaaga ccaactacaa gctgcgcgac
      121 tgggtattct cccgccagag atactggggc gagcccattc cgattgtgga gtgccccaag
      181 tgcggctatg ttccgctgcc cgagagcgag ctgccgcttg agcttcccaa ggtagaaagc
      241 tacgagccca ccgatacggg cgagtcgccg cttgccaagc tcacagactg ggtaaatacc
      301 acatgcccca agtgcggcgg cgcggcaaag agggaaaccg acaccatgcc ccagtgggca
      361 ggctcaagct ggtacttcct gcgctactgc gacccgcaca acgatgagtg ccttgcctcc
      421 aaggaggctc tcgattactg gacacccgtc gactggtaca acggcggcat ggagcataca
      481 accctccacc tgctttacag ccgtttctgg cataagttcc tttacgacat cggcgttgtg
      541 cccaccaagg agccctacaa aaagagaacc agccacggca tgattctcgg cgagaacggc
      601 gagaagatga gcaaatcccg cggcaatgtg gtaaatcccg atgaaatagt aaacacctac
      661 ggcgccgaca ccatgcgcct ttacgagatg ttcatcggcg actttgagaa ggccgctccc
      721 tggagctcca cctcaatcaa gggctgcaag cgttttgtgg acagatactg gagcttcaga
      781 gacatcctca tccccgacaa aaagattaga gatgagcttt taagctcctt ccacaagaca
      841 atcaagaagg tcacgcacga cattgacaat cttaagatga acaccgcaat agccgccctt
      901 atgagcctta tgaacgaaat ctccgaaacg ggcaagataa caagagagga actcaggata
      961 tttacaattc ttctcaatcc ctttgctccc catgtcaccg aggaggtttg ggagcttgaa
     1021 ggctttggcg gcagggtaac cgacgcgaaa tggcccgaat actccgagga gctttgcgag
     1081 gagaagaagg ttgagattgc ggttcagatt aacggcaagg tacgctcaag aatcgtaatc
     1141 gacgccgacg ctacacagga gcaggctata gccgacgcta aagccgacgc cagaatcagg
     1201 gaggagctct caggcaagac ggttctgaag gagatttatg taaagggcaa gctcgtaaat
     1261 atagtcgcaa aataaattaa agccgaccga atttcggtcg gcttttatat tgcaaattta
     1321 aaggcgggtt ttccccgcct tttttagctc agctcaaact gacccgtata gagggcgtag
     1381 tatctgccct tcttttcaat cagtgtgttg tggtcaccct gctcaattat ctcaccgttt
     1441 tccagcacta taatctggtt ggagtttcta acggtggaga gcctgtgagc gattacaaac
     1501 accgttctgc cttccattag gctgtccatg cctttttcca caaggtgctc ggttctggtg
     1561 tcaatggagc ttgtagcctc gtcaagaatg aggaccggcg gattggcaac agcggctctg
     1621 gctatggata taagctgcct ctgaccctga cttaagttgc cgccgtcgct tgtcagcatt
     1681 gtctgatatc cctggggcag cctttctata aatgaatggg cattggcgag ctttgcggcg
     1741 ttaacgatat cctcaaatgt agcgtcaagc ctgccgtagc ggatattgtc ctcaaccgtg
     1801 cctgtaaaca ggtgggtatc ttggagcacc atgccgaggg agcggcgaag atccgctttc
     1861 ttaatcttct taatatctat gccgtcgtag gtgatttcgc cctcctgaat gtcatagaag
     1921 cggttgatta aatttgtaat cgtggttttg cccgcgcccg tagagcctac aaaagcgaat
     1981 ttatgacccg cggcgccctc gatattgatg ttcttcagaa cgggcttatc cttgtcgtag
     2041 gcgaagctta cattcttaaa ccttacatcg ccctttacaa gagtgtagct tacgctgccg
     2101 tcgtcattag gctgcttcca agcccaaacg ccggttctgt agtcaacctc ctcaatgctg
     2161 ccgtcgtcat tgactttagc gtttacaagg gttacgactc cgtcgtcggt ctcggcaggg
     2221 tagtcgataa tctcgaatat cctctcagcg cccgcaagag cggcaagaat attgttcacc
     2281 tgctgggaga cattggcgat gggcattgaa aactgccttg tgtactgaag gaaggaggca
     2341 agctgaccga tgctaagccc aataacgccc gtagccgctt tcttgatgat gagataagcg
     2401 ccaatggtgg ctgtcagagc atagttcata taagagaggt tgcccatgac gggcataatg
     2461 acgcttgcga aggtgtgggc gttgactgaa gcggatttca gatcattatt tagcttgccg
     2521 aaatcctcaa taaccttatc ctctctgcag aaggctttaa caactctctg accctcaatc
     2581 atttcctcaa tataaccgtt taccgcgcct accgttcttt gctggagtct gaagtacttt
     2641 gcgctcctgc tgcctatggt ccttatggca aagagcatta tggcaagcat tatcaaaatc
     2701 agtatcataa gcgcgggact gaggataacc atcattataa atgtgcccgt aaccgataca
     2761 agcgaggtca gaatctgggt aagaccctgg ctcagagcct ctcttaaagt gtctgtatcg
     2821 tttgtaaagc ggctcataat ctcgccgtgg gtgttcttgt caaagaagtc aatgggcaaa
     2881 tcctgcagat ggtcgaacaa atcccgcctt atggcgttga gcgtcctgtg ggtgagtccc
     2941 accatgaccc tttgatatgc gtactgagcc gcggctcccg caatatagat gacggcaagg
     3001 atggctatat agcctaaaat ctttgcaaca gggtcggtga tttcgccctt aaaagccttt
     3061 tcaatggtgt tgatgacggg ggtgagcata taggttcccg caactcccgc gccggaggca
     3121 ataagcacaa ggaaggcgac caaaaccagt atcagcttat tctttgtaat atacttaatg
     3181 agccttccaa ccgtcttttt cgtgttgaca ggcttcagac ccggagcgcc cctgccgggc
     3241 cctctcatag gtgctctcat aggtgctctt gtgctcatat ttccgaatcc tcctgctgtg
     3301 agcggcaaac ctccgcataa atgccgccca attttaacag ctcctcatgg gtgccgattt
     3361 cgctgattct gccctcgtca agaactataa ttctgtccgc gtgctttacg gagttaaccc
     3421 tctgggcgat tattattttc gtggtttcgg ggaagtagtt tttaaggctc tctctgatga
     3481 gagcgtcagt ctttgtgtca acggcgcttg tgctgtcgtc aaggatgaga atcttcggct
     3541 tctttaaaag agtcctcgcg atgcaaagcc tctgcttctg accgcccgag acatttgtgc
     3601 cgccctgacc aagcatcgtt tgatagccgt cggggaaact catgataaag tcgtgggcag
     3661 ccgcggcctt gcaggcggtg ataatctcat ccatagtggc gttttcgttg ccccagcgga
     3721 gattctcctc aatggtgccg ctgaaaagca gattcttctg cagcaccata gccacagcgt
     3781 cacgcagctt gcggagctta tagtccttta cattcctgcc gccgacataa acggctccct
     3841 cggttacatc gtagaacctc gggataagct gcaccagcgt ggacttagaa gagcccgtgc
     3901 cgccgataat gccgattacc tcacccgatt taatagaaag attaatatta tcaagaacat
     3961 tcttcttcgc gctctttgag tatttgaaag acacattgtc aaacacaatg ctgccgtcct
     4021 gaacgcttaa attctcgtca gcgtcgtcgt ctttgataat gggctctgtg gacagaacct
     4081 caatggccct ttgaatcgac gcccttgaaa tgacaatcat gatgaaaacc atcgaaacca
     4141 tcataaggga gaagagaatc tgatgaacat aggcgataaa gctcataagc tcgccggtgc
     4201 ccatggagcc ggaaagcacc atgttgccgc cgaaccaaag aatcagcgtg attgcggtat
     4261 acattgaaag ctgaagtatc gggttctgaa ggattgccag cttttccgca aagatgctgg
     4321 cgtctcttaa ttcatcgttg cttattctga acttttcctt ttcatgctcg gctcttacaa
     4381 aggtcttaac aaccctgatt ccgataaggt tttcctgaat agcggcgtta aagcggtcgt
     4441 atttctcaaa catcgcccta aatcttggga aggctttaag tatgatgaat acgagcataa
     4501 agccgagaat cggggcgatt acaaggaaga ttatagacag ccttgcgttg atttttatgg
     4561 catagataat agccaaaagg aacataaaag gagcccttgc gcaggtcctt attatcatca
     4621 taaaggtcat ttgtataagg tttatatccg tggtcatcct cgtgataagc gaggcggtgt
     4681 ttatggagtc aatattgtcg aaggagaagt tctgcacctt cttaaaaagg cccattctca
     4741 aatcggcgcc aaagcccatg cctgcgactg cggcaaatct cgcgccgagt attccgaggc
     4801 aaagggaaac aatagacaaa gcaaccataa cggtgcctgt cctatatata agctgagtgc
     4861 cggagcccga tataatgccc atattaatga gggtgcgaat taggaatagg tccgcggtct
     4921 cgctcaaatc cttgccgtaa agtccgctgt ccacaataac ggacataagc gccggtatgc
     4981 agacctcaag catagcctcc agcattaccg tcaggggcgt aagtatagca tacagctttt
     5041 gatttttcat aaagggagtc agtttcttta agttcatacg cttgtcctct caggttccga
     5101 aattagtatt gtcagattat tgctcctctt tggacagatt ctcaatcatc tttacaagca
     5161 ttctgtcaaa ctcgtttttt tcgttctcgc tgaagccgtc cagcatttca tcggacaaag
     5221 ccttcagctc gtttctgcag tagttgtgag cctctacgcc cttttctgta agccgtattt
     5281 tgttaaagcg catatccttt tcatccgtca gcttttcaat cagtcccatg ttctgcatgc
     5341 gccttatgct tacagcgaca gaggcggggg aggtcattat cttgtcgcaa atatccttct
     5401 gggagcatac accgaactca tttattatat gaagtatagc cggctgcccg gggtaaatat
     5461 ccctttcctt aaaacgctga tgcagccgct ttctgaacag tatatgggtc acaatgaatt
     5521 tatccgctgt tataagttcc ttcggggaca attttttcac ctcgtcttgc ccaaaaatat
     5581 agttagccgc ctaatagtta gccgactaac tattaaataa tatcagcata tttttttaaa
     5641 gtcaataaat ctttccgata ttttcatgtt tttgttaact gcgataaagc gatagagggc
     5701 aaaaagtgcc gacttatgca agtctaatga atatttttcc catatgcttg caaataaagc
     5761 ataaattatg cgacaataaa accatagaga taatctaatt agagaataat cattacaaaa
     5821 gaataacatt ttcttacgat tatcttaata tttcccttat tcccttgact gagggtagcg
     5881 ttcatataga atacaagtag aataatcgtg acagagaaac tttatgaggt ttgacagccc
     5941 tgtctttcct caaaaggagg agtagctttg atagaggcag taaatgtaac aaaaaaattc
     6001 ggcgatttca cagctatcga aagcctgtcc ctgactgtcc ccaaaggctc aatttacggt
     6061 cttgtgggat acaacggcgc aggtaaaacc actctgctta aaacaatcgc gggagtctac
     6121 cgccccgaag gcggaaaggt tttgatagac ggcgagcccg tttttgagaa cgaaaagaaa
     6181 aagcaaagac tcttttatgt tcccgacgaa atttatttca agccctacgc cactatgaac
     6241 aagatggcag ccttctacaa aggctactat cccaattttt catacgacac ctttgaaaag
     6301 gtaacaaagc ttttcggtct tgaccccaca aaaagactga acggcttttc aaagggaatg
     6361 caaaggcagg cggaggtggt tttcgccatg tccacaatgc ctgaaaccct gctgcttgac
     6421 gagtcgtttg acggacttga cccgcaaaag agagcggtcg tcaaggaact tctgtacgac
     6481 tacatgagta ggaaggatgt cgcgaccatc atatcctccc acaacctcca cgagcttcag
     6541 gacctctgcg actatgtggg acttataaac ggcaagagga tagtcctcag cggctccgta
     6601 tccgacctgt caaagggcaa ggcaaagtac aggctggtat tcgaccgccc cgtaaccgag
     6661 caggatttca aagacattcc ccacaagcgt cttaacatcg acggcagcat cgtaacactg
     6721 atggccctcg gcacccctga ggaggcatac gcaaagctta aagcgatgtc gccgctgctt
     6781 atagaagaaa cactcctgac acttgaagaa atattccttg acgaaatgga ggctgagggc
     6841 aatgaagcaa aaaagatctt cggctaaccc ctttacccat accttttctg aaagcctgaa
     6901 aagcacaaat atagtcgtaa cactacttat gcttgcggta atggccttta tgttcttttc
     6961 aatgctgagc tacccgcttt catatcgcga cccctatatg gaatcaccca ctgccaagga
     7021 ccagtatata tattatctgt ttgccccaaa cgaactggca acggtgttcg cctgtggact
     7081 tatgcttgtt gcgggcattc ttatgggact ttcctcattc agattcatca cttccaaaaa
     7141 gaccgtaaat gtatactaca gcctcggcat taagagagaa aaactgttcc ttgcccatta
     7201 cctaagcggc gctttgctgc ttgtggttgg agccgtgctt tcggtaatgg tgggcgtggt
     7261 tatgaacctt atctatatag gcagcagctt tgagctgtgg caggcggcca cctactatat
     7321 gatggcattt gccacaatcg cccttttctc atactccgta acagcagcgg ttctctcaag
     7381 cgtgggaacg gtttttgagg gcggaatatt ctcggtcata atctcattcc tccccacatt
     7441 cctgctctca agcgttcaat tccttatgaa caagctggta tggggcaatc cgttcggtgt
     7501 tgccttcgta gaaacgggct taacgcacca atataattcc ccgtcgcttg taacccattt
     7561 ctcatacctc actcctttaa ccgcgttttc aagtaccctt gtggaaatag ccgaccgtga
     7621 aaagcctctg aatgctgacc aggccgaaaa ggctgcggaa acaatatatg aactggcagc
     7681 gtcggactac ctgaccgatc ttttctggct gattgcgatt gcggctatat tcttcctcgg
     7741 aatgtacctc tttaagcgca gaagagctga aatcagcggc tttatcggca ccaacagagt
     7801 tcttaacaat gtaggaattt ttgttgtggc aattttcact ttcagcgtta ttttcacaag
     7861 ctcaccttta cagaaatatg cagctttcgg aatagccctt tttgccgcgc ttttggtctt
     7921 tgtcatactt gatgcggttt taaggcgcag ctttaaagag tttaagcggg accttgtatt
     7981 cctgcccatg catctcgcag ttattacagc catcttcttc attttctcaa cgggactttt
     8041 cggctactca aagagagttc ccgccgctga ggacatcaaa tccgtttccg tctccactgt
     8101 gggcgttcag agcttcttca gcgagtatta tcccatgcgc gaggatattc tcgtctaccc
     8161 cggctcaaac gaaatgattt ccggctttga gagcgagaag gatattaagt taatcagaga
     8221 tattcacgca aaaatcgccg agcttaacgc cgcggacgcg tatgagatgt ggaagttctc
     8281 cgctgaccac tcctcaaagc gccttgcctc cagaattcag ttcacatact atctcaagaa
     8341 cggcaagacg gtcagaagga gcttcaggaa tgtctccgcg ggcgtaatag aagagctttt
     8401 aaagatttac gacacagaaa gatttaaaaa ggctgccgat gaggcgctgt ctcccgatac
     8461 ccccatcatg cagaacgaga gggcaaggct cctgcgttca gaaggcgtaa ttgtaagagt
     8521 ttatccgaaa gacctgtcat atatcgcaag gctgaacctg acttcagagc agagaaacga
     8581 gctgtatgaa gctctcaggc aggatatcgc agagcagact tacaaggaga gatacttccc
     8641 cgaaaagccg cccctcggag taatcagcat tgtcggcaat aactatacgg attttgacaa
     8701 gggcataatc gaggtcatag aagacgaaaa cggaagagct tacgaaaaag ccatagagcc
     8761 cccgtcaaag gctgatgata tcataaagcc tccctccgga gaaccgtggg atattctcga
     8821 caactccccg acaatcgcaa tgcacggcac cacggatttc aacatcgact ttgtaatcac
     8881 cgaggacatg gaaagaacca ttaagttctt aaaggacaac aatatgattg acgcctttaa
     8941 gggcagcgaa aagaaataca cctcggtaca gctcgtttca atcgagcgca gctttacctt
     9001 ccagcccttc atgctcaaat acggctttga cttccactat gacgccttcg gcaagtccat
     9061 aagcaaagct aagagcaatg aagaataccg ttacgatccc cttgagaatc aaaagaccta
     9121 caccgtaacc gacgctgaaa caattaaggt attgaaggat atctcatacc tcacttactt
     9181 cagcagcggc acgggctatg cggcggtatt caacgcggag gacggcactg cccagtatct
     9241 ctacatcccc gcttcaaaga tgcccgcggc tttagccggc gccgttagga actttgacac
     9301 agagcagctc cgggatttta acctcagcaa ttattaaata aaacgggcgc ccaagcggcg
     9361 cccttctttt ttaatgggga atgtcgacat ttgttgacat ttccatattt ttgtaataaa
     9421 atcctgacaa gctctaaggg gaggcaaaaa ggtggaaaaa aagaaggcat tcactttaag
     9481 cgaaaaagtg cttgtgggtg taataatcgc ccttgctttc ttgcttgtcg ctcaaagaat
     9541 cagcgcgtac agatacttct caaggcttga cgccatcgaa ataatagcct ctgaggagaa
     9601 aacagccgcc gagacggctt ccgaaacgaa aagcgaggaa actaagagtg aaacggaaac
     9661 cgaatctgaa gctgaacccg aaaagcgtct tataaacatc aacacagcct caaaagagga
     9721 gctgattctg cttaaaggaa taggcgagaa aaaggctcag gcaataatcg attaccgaaa
     9781 cgaaaacggc ggctttgaca agattgaaga aattatgaat gtcagcggaa tcggtgaaaa
     9841 gaccttcctg aacataaaaa gctatattac ggtagattaa gaacgctcag gcgttctttt
     9901 ttcttttgtt gttttttttc aaagtttgtg tgatataata cttataaatt aactaaaatt
     9961 ttcataattt gtgctatttt gcataatgct tttcttttgg aggaaactta atgagcggca
    10021 gccatttttt tgccttcttt gcgagaatga aatatattaa ccgctggtct ttaatgcgca
    10081 atgtcttcta tgaaactcta agccagcaca gcactgaggt ggcggcaatc gcccatatgc
    10141 tggctttaat cggcaacaag cgtttcggca aaagctacaa cgctgaaaga gcggcggttt
    10201 tagggctcta tcacgatatg ccagaaatcc ttacaggcga tatgcccact cccgtaaagt
    10261 actttaacag cgagataaag aatgccttta agtccgttga gcgtgaggcg gcggcgagac
    10321 ttaccgactc cctgccgggc tatttaaggg acgagtttaa gacaatcttc agcgaatcaa
    10381 gggacgacgc ggaggagtgg cggcttgtta aggccgcgga caaaatagcg gctctaatca
    10441 agtgcattga ggagcaaaag gcgggcaata ctgaatttga caaggcaaag cagtctgtgg
    10501 aaaacactct caaagaaatg gaatgccccg aagcggaagt ttttatagag gagttcctgc
    10561 ccagcttttc actgaccctc gaccaactta aataacaaca aatatcggag gatattatgg
    10621 atataaagaa tattctcgaa aagctgataa gctctaaggg cgtatcggga aacgaggcat
    10681 tggccgctaa tacggcggct gaaatccttg ccgactatat gcccaccgag attcaaagca
    10741 gcggcaatgt tgtgggcttt cttgacggag aaggccccat tataatgctt gaggcgcacc
    10801 ttgaccagat aggcctgatt gtcacagata tagacaaatc gggctttatc cgctttgcca
    10861 agtgcggggg agcggacatg agagtcctgc ccgccgccga tgttaccata gtcggcaaag
    10921 acggggtcga gattgacggc attgtaatca gcacaccgcc ccaccttgcc aaggacgagg
    10981 acaaaaagaa gtttaagcaa atagaggacc tggcgataga tgtgggtctt tgctatgagg
    11041 acgcattaaa ggttatccgt cccggtgaca gggtaaccta caaaaaagcc ttcggcagct
    11101 tgatgaacaa taggctttat ggcacctcct ttgacgacag ggccggagtc gccgcggttt
    11161 tgaaggctgt ggacctatta aagggacaga aattaaactg caaactctgc gttctctttg
    11221 cagcccttga ggaaaccacg ggaggcggcg cggctgtcgg agcgttcgct catcagcccg
    11281 acgagtcaat ctcagtggat gtcagctttg ccgaatcccc cggagtgccc gagcacaaaa
    11341 cgggcaaact gggcggcggc gccatgatag gcattgcccc gactttggac gatgaaataa
    11401 gcggaaaact tattaagatt gcgagaacca aaaaaataaa gtaccagacc gaggttatgg
    11461 gcggtcagac ctccaccaac gccgacaaaa taagcgtagc gggcagggga gtcaagaccg
    11521 gtcttatctc aataccactg agaaacatgc actcagcggt tgaagtgctg cagcttgacg
    11581 atgttgaggc tgtggctcag ctcttagcgg aatatgtact gtcaaagggg gttatgtaat
    11641 gctggagctt ttaaagcaga tggctgaaat cacggctgtt tcaggcaaag agggagttct
    11701 cagagattta atcatagagc agattaaacc ctttgccgag tacgaaatcg acccccttgg
    11761 caatctcctt gtccgcaaga agggcaggcg gaaggctaag aataaggtta tgcttgccgc
    11821 ccatatggac gaagtcggaa tcatagtaac ctatgccgaa aaaagcggct ttctccgctt
    11881 tgccaccgtc ggcggaatcg acccgaaggt gctgatcggc agagaagtag tctttgaaaa
    11941 cggcacaaga ggcgtaaccg ggctctctcc cgtgcacctt ctcaaagact cagaaaaaga
    12001 cgccgttccc gaaatttcct cactttatat tgacatcggc gccgaaaatg acgagcaggc
    12061 tctaagcgcc gttcccttgg gaacaagggg aacctttagg gaaaaattcg aatatttcgg
    12121 cgacggtctt atcgcgggca gagcgcttga cgacagaatc ggcgtcgccc ttatggttga
    12181 tataatcagg agtgaaatag aatacgacct tcactttgcc tttacggttc aggaggaggt
    12241 aggcgcaagg ggagccgccg cggcggcatt cagcgtcgcc cccgactacg cgatagtcct
    12301 ggaggcgaca acggcggcgg acattataga tgtgcccgag cacagaacgg tctgcgcatt
    12361 aaagaacggt cccgccgttt ccttcatgga ccacagcacc gtctatccca tggagcttta
    12421 tgaccttgcc atgaaagtcg cgaaagacaa cggcatcaag gctcagccca aggcggctgt
    12481 tgcgggcggc aacgacgccg gctcaatcca caagagcggc aagggcgtaa aagccataac
    12541 cctcagcgtc ccctgcaggt atatccattc ccagaactgc gtcgccgcgg tgcaggatat
    12601 ggagtccgcc agggctttgg ctgaaaagct ggccgctgag cttgccaatg cttaagcttt
    12661 taaacgacga cacctatttt caggcaaggc gacagcttga aaataaccaa ttcggagccg
    12721 taatcggctc gctttacaaa gcctacggaa attcctgcga ctactgcgtc tgcctatgcc
    12781 agaaaagcga cgacggcaga atcaccgcgc tcatttccgt tttcatgggc agcatgaccc
    12841 tttactgcga aacagatgcg gattttgaag agctgcggct gtatataaaa agcgcatccc
    12901 cctttgaaat
//
LOCUS       DAONVQ010000047        12838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3738_length_12838_cov_4.370727, whole genome shotgun sequence.
ACCESSION   DAONVQ010000047 DAONVQ010000000
VERSION     DAONVQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3738_length_12838_cov_4.370727"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLD98_04390"
     CDS             complement(<1..509)
                     /locus_tag="PLD98_04390"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQA05521.1"
                     /translation="MFKNREMTDDFRSEVLKNGEAKISARALMKGAGLTDDQIYRPFI
                     GVVNSYSNFFPGHSNLDKIGQAVKDGILMGGGTPIEFSTIGICDGIVMGGLGMRYSLP
                     SRELIADSVETMANSHTCDALVLVCSCDKIIPGMIMGALRLDIPFIVVTGGPMLAGNY
                     RGKRVDVQDV"
     gene            complement(555..1586)
                     /locus_tag="PLD98_04395"
     CDS             complement(555..1586)
                     /locus_tag="PLD98_04395"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HQA05522.1"
                     /translation="MKTILVGTGAIGGTVAVLAKANGFDLHVVCRGEETAEKIKNDGL
                     YLKGPRREHKVKLHAYPDIESIDQHDYDICIIASKAYAMPEIAKAMLPHIKEDALVVS
                     MQNGMCLEILADIVGKERAVGCMIGFGANLDTPTSLTMTSLGSFAIGMLPGYTSDKLE
                     YIRKMLDCTVPCHISDMIQSEQYSKLIINSCITSLGALTGQTLGVMLDEKSARVLFLA
                     IAREGIKVAKAMNLYVPPFNKVLNYNLLLLTDAKWFDLLCETLFRVIGKVKYKDDKSS
                     SLQSLERGKPTEIDYFNGYIVRKGKEFGVPTPVNEKMVQLIKEIERKERKISMDNLKV
                     FVDEGLIKI"
     gene            1857..3932
                     /locus_tag="PLD98_04400"
     CDS             1857..3932
                     /locus_tag="PLD98_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQA05523.1"
                     /translation="MKVYNAKDIRNIVIAGHGGRGKTTLAEAMLYVAGATDRLGKVAD
                     GNTVMDYDAEEKRRKTSVSAAVAPLEWKNRKINLIDTPGLFDFEGAVHEGMRAAECAL
                     IVLAAGAGYDVGAEKAFDAADGRGIAKMFAVTRCDQENTDFAKTLEALKAEVGTKICP
                     VVVPYYEGGAVKAYANLATGKAYEYKNGKASKTAFPEDAAISEMRDVFNEAVASADDE
                     LMEKFFEGESFTAEEIQRGLSLGISNGSIYPVFACAGLTADGVDLLLDAIADYAPNAA
                     EHAGETATDASGAEKELACDENGPLAAVCFKTVADPFVGKMSFIKVVSGKMTADVPAY
                     NARTGESERIGKLLSVKGGKQEDISVLPAGDIGVVTKLSSVMTGDTICSPKDVLHLKG
                     VQFPTPCLSMAVKAVKKGEEDKVSSGLRKLMEEDPSIVFYTNEETRQQILSGLGEQHL
                     DVITTKLQAKFGVKVELEIPRVAYRETIRKKVEAQGRHKKQTGGHGQFGDVYIRFEPW
                     DTEDLVFAEEIVGGAVPKQYFPAVEKGLRECVAKGFLAGYPMVGVKATLYFGSYHPVD
                     SSEMAFKIAASLAFKNAMPQASPTILEPIGTLKAYMPDENLGDIMGEITKRRGRVLGM
                     GPAKKPKMQELTAEVPMAEMGDFSTTLRSVTQGRGSFTLEFARYEEAPANIVEKVIEE
                     AKKRQEEEE"
     gene            complement(4216..4419)
                     /locus_tag="PLD98_04405"
     CDS             complement(4216..4419)
                     /locus_tag="PLD98_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05524.1"
                     /translation="MSKDKKKNMADMPLGLTSALAKDIDALTQFASLSETDKEKFIKG
                     ASQVSSKQEMEAYVRQLSDNAMI"
     gene            complement(4436..4630)
                     /locus_tag="PLD98_04410"
     CDS             complement(4436..4630)
                     /locus_tag="PLD98_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05525.1"
                     /translation="MAKQGMKRPGADELGIKYPKNVVEPVPEIQGKAKSGKKSAGNNQ
                     WAATQPHNDFRERRDGPGGN"
     gene            complement(4721..6577)
                     /locus_tag="PLD98_04415"
     CDS             complement(4721..6577)
                     /locus_tag="PLD98_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HQA05526.1"
                     /translation="MEIAFNSRDRKYKSVFGAIKTNESTGFTLVLHSSLLAEKAFLVI
                     WPDGKKEEYIPMTKSENGEFHFWRTEFKTPNKGLYFYKFDYDTPYGRGTVLNSGSGIG
                     YVGREGVPWQLTVYDENFSTPDWLKGKVAYQIFPDRFRFSGEKKENVPKDRELRSDWG
                     GTPKWYPDENGRFNNDYFCGDLKGIEEKLEYLASLGVGLIYLNPIFEAHSNHRYDTAD
                     YFKIDPLLGTAEDFVSLTKKAEEYGIKIILDGVFSHTGADSVYFNKYKRYGDGGAYND
                     PESPYYSWYKFGETRDDYASWWGVKTLPEVNENEESFKEFITGDRGVVNHWLKNGAYG
                     FRLDVADELPDIFIEKVRETVKKHKPDALLWGEVWEDASNKISYGGRRKYLLGDELDG
                     VMNYPFREAVIDFMLGGSAELFMERIVTICENYPKPALDASMNFLGTHDTARVLTILS
                     GAQLSHIDKNRQAEFSLTDSEKAIAKKRLLCAASLIYALPGVPTIYYGDEAGLEGGVD
                     PFNRRCFPWGNEDEEILNHFKALGEIRSSHDAFKEGEFVPVSAMLGCVAFIRKNEKGE
                     SVFVISNKNHHEITYYLPENIKNPKALIGGAVTNDKKGVNIDSETTAILSFN"
     gene            complement(6582..9032)
                     /locus_tag="PLD98_04420"
     CDS             complement(6582..9032)
                     /locus_tag="PLD98_04420"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HQA05527.1"
                     /translation="MEYKLTKAKAKELLESKLLHFFGVGAESASYEHVYRALAMILTE
                     MMNRQRREFEKEAREKNAKDIYYLSMEFLMGRSLKNNLYNLDLLNVFDEVLSDYGISL
                     DKVFEIEPDAGLGNGGLGRLAACYLDALATQGYNATGYSILYEYGIFKQRLVDGWQTE
                     LPDFWLSGGDAWLVKREESAVDILFEGWVKDEWHEKFHHVELIDCQKIKAVPYDMCVS
                     GKGGKGISVLRLWSAVAPGIDMALFNEGNYLRAMENASMAEIISKVLYPADNHPEGKS
                     LRLRQQYFLVSAAIQDIVRKHLRRYPTLDNMPEKVAIHINDTHPTLAIPELMRILLDE
                     CGYGWDDAWRIVTNTIAYTNHTVMREALEVWSEDLLKRLLPRIYQIIKEIDNRFRSFV
                     WNSSHDPDLVESTAVISDSAVRMANLCIAGSHSVNGVSELHSGILKDTVFNDYYKLMP
                     SKFKNVTNGIAHRRWLCQANPLLANFIKELIGDSFVYNGDELINLRKYADDKSVLDEL
                     NKIKLKNKERYADYIMKKMCVEIDPESIFDVQVKRLHEYKRQHLNALNILNKYLEIKE
                     NPNGNYIPRTYIFSAKAAPGYFLAKKIISFISALADLINNDSEVNKYLKVIFVENYSV
                     TEAELLMPAAEISEQISLAGTEASGTGNMKLMLNGAITLGTMDGANVEIYDAVGEENI
                     IIFGLKEDEVKHLRNIGYKPSDYYFNNKDLKRTIDFINSGGVGGKSFDEISKSITNYD
                     PYMVLADYADYRLAQKRSDELYSDREKWNRMSLINISGAGRFAADRAVRQYAENIWGT
                     PSAFGFENLRSRVNDDSR"
     gene            9435..9635
                     /locus_tag="PLD98_04425"
     CDS             9435..9635
                     /locus_tag="PLD98_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05528.1"
                     /translation="MKVLSKSYEQYCCVLERNVVMEEATYHNGGIKLFCTNYPQCFTC
                     GGCKNIILNDKIEKAAMVNTMI"
     gene            9839..11335
                     /gene="thrC"
                     /locus_tag="PLD98_04430"
     CDS             9839..11335
                     /gene="thrC"
                     /locus_tag="PLD98_04430"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQA05529.1"
                     /translation="MKYTSTRDSSLQISSAEAIVKGISPDGGLFVPTEFPKVDGDFIA
                     SLTKMNYKERAKAVLSLFLTDFSEDEISGFVEKAYSEGKFESHEVAPLKILPNGVSFL
                     ELWKGPTCAFKDMALQLLPHLLTGSAKKIGDDSLIVILTATSGDTGKAALEGFKDVEG
                     TKILVFYPSEGVSDIQKLQMATQEGGNVGVAAIYGNFDDAQSGVKDIFGDKALEAELL
                     SKNIKFSSANSINWGRLVPQIVYYFSAYCDLVNRGKVKYPEKINICVPTGNFGNILAA
                     YYAMRMGLPVNKLICASNRNNILTDFIKTGVYDRNREFFTTTSPSMDILISSNLERLL
                     YELSGRDDAKIKEWFTSLKENGKYEVSPEVLNELKSVFESGYCGDEETAAEIKRVYDE
                     FGYLIDTHTAVGSFVYENYRKTSGDDTPVVIASTASPYKFCSSVLSALEGRDSSGENE
                     FEIMRRLEKLTNTAAPKQLKNLEGVSPRFTEVYDKKDMRQAVFSMLNI"
     gene            complement(11455..11631)
                     /locus_tag="PLD98_04435"
     CDS             complement(11455..11631)
                     /locus_tag="PLD98_04435"
                     /inference="COORDINATES: protein motif:HMM:NF019199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HQA05530.1"
                     /translation="MGEKRHVINNIKCEVKNCVYQTPEQRCTAGEIEIGTRNAKDCSE
                     TACITFELDKHANC"
     gene            11836..12528
                     /locus_tag="PLD98_04440"
     CDS             11836..12528
                     /locus_tag="PLD98_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA05531.1"
                     /translation="MSVFAIGDTHLSFGTDKPMDVFRGWTSYTEKLRENWIKSVGEND
                     TVVIPGDISWAMRLEESEKDFEFLHSLPGEKIILKGNHDYWWSTKGKMDAFLSSKGFH
                     SIKILHNNAYKVGDFAICGTRGWFFDDDEPHSEKIILREAMRLKTSLECAMKLGGELL
                     VFLHYPPLSSTQKCDEIYNILLQYPVKRCFYGHIHADMTEKYSYFFKDGIEFKLVSAD
                     YLKFSPLLIEEF"
BASE COUNT         3322 a         2969 c         3028 g         3519 t
ORIGIN      
        1 acatcctgaa catctactct tttgcccctg taattgcccg ctagcatggg gccgcccgta
       61 acgactatga aggggatgtc aagtcttaaa gcgcccataa tcatgccggg gataatcttg
      121 tcgcaggagc agacaagaac aagggcgtcg catgtgtggg agtttgccat ggtttcgaca
      181 gagtcggcaa taagctctct gctgggcagg gagtatctca tgccgagacc gcccataaca
      241 atgccgtcgc aaatgccgat tgtggaaaac tctatgggag tgccgcctcc catgaggata
      301 ccgtctttga cagcctgtcc gattttatca aggtttgaat gtcccgggaa aaagtttgaa
      361 taggaattga caacgcctat aaagggtctg taaatctggt cgtcggtaag gcccgcgccc
      421 ttcattaagg ctctcgcgct gattttggcc tcgccgttct ttaaaacctc gctgcgaaaa
      481 tcatcggtca tttctctgtt tttaaacata ccgttttccg cttaaagcgg aatgttcacc
      541 ccgattctat gtaatcagat ttttataagg ccttcatcaa caaagacttt gaggttgtcc
      601 attgagattt tgcgctcttt cctctcaatc tccttaatga gctgaaccat cttttcgttt
      661 acgggagtgg gaacgccgaa ctccttgccc tttctgacta tgtagccgtt gaagtagtca
      721 atctctgtgg gcttgcccct ttcaagggac tggagcgagg aggatttgtc gtccttatat
      781 tttaccttgc ctatgactct gaaaagggtt tcgcaaagaa gatcgaacca ctttgcgtcc
      841 gttaaaagaa gcaggttgta attgagaact ttgttaaagg gcggtacata gaggttcata
      901 gcctttgcga ccttgatgcc ctcccttgct atggcaagga aaagaactct ggcggacttt
      961 tcgtcaagca ttacgccgag ggtctgaccc gtaagagcgc ccaatgaagt gatgcaggag
     1021 ttgattataa gctttgaata ctgctctgac tgaatcatat cggagatatg gcagggcact
     1081 gtgcagtcga gcatttttct tatgtactca agcttgtccg aagtatagcc gggaagcatg
     1141 ccaatcgcaa aggagccgag gctcgtcatg gtaagagatg tgggcgtatc aaggtttgct
     1201 ccaaagccta tcatgcagcc aaccgccctc tccttgccca cgatgtccgc aaggatttca
     1261 aggcacatgc cgttctgcat ggatacaaca agcgcgtctt ccttaatgtg gggcagcatt
     1321 gcctttgcta tttcgggcat ggcgtatgcc tttgaagcta taatgcaaat gtcgtagtcg
     1381 tgctggtcga tagactcaat atcagggtaa gcgtggagct ttaccttatg ctctcgtctg
     1441 ggtcctttga gatacagtcc gtcgttcttg attttctccg cggtttcctc accgcggcag
     1501 actacatgca aatcaaagcc gtttgctttc gcaaggaccg ctacggttcc gcctatggcg
     1561 cctgtgccga ctaaaatggt tttcacttta tttcctcccc aatataaaat tagcaaatat
     1621 caccattttt aatgattaca taggagatta tatattaaca ttcaacaagt gtcaatgatt
     1681 tacatttaat tatgacaatg atgtgaaaaa tgaaatttta ttaagaaatt acacaaaata
     1741 aggctcatat ttgtagtttt tttaattgaa aagtataaat aattatgata caattcagtt
     1801 tgatacttta atatgacgat tagtctattc agaacttgat gggaggatta aggattttga
     1861 aagtttataa cgctaaagac attcgcaaca tcgtcatagc cggtcacggc ggcagaggca
     1921 agaccaccct tgctgaggcc atgctttatg tggcaggtgc taccgacaga ctcggcaagg
     1981 ttgccgacgg caacaccgta atggactatg acgctgaaga aaagagacgc aaaacttcag
     2041 tgagcgctgc ggttgctcct ttggagtgga agaacagaaa gataaactta attgacactc
     2101 ccggtctttt tgactttgaa ggcgctgttc atgagggtat gagagctgcc gagtgcgctc
     2161 ttatagttct tgccgcgggc gcaggctacg atgtaggcgc agagaaggca tttgacgccg
     2221 cagacggcag aggcatcgct aagatgttcg ccgttacaag gtgcgaccag gagaatacag
     2281 acttcgcaaa gacacttgag gctctcaagg cagaagtagg cacaaagatt tgccccgttg
     2341 tagttcccta ctatgagggc ggcgctgtta aggcatacgc aaaccttgcg acaggcaagg
     2401 cttatgagta caagaacggc aaggcaagca aaaccgcctt ccccgaggac gcagccatca
     2461 gcgaaatgcg cgatgtattc aatgaggcag tagcatccgc agacgatgag cttatggaga
     2521 agttctttga gggcgagagc tttaccgctg aggaaattca gcgcggactt tccctcggaa
     2581 tttcaaacgg ctccatttat cccgtattcg cttgcgcagg acttacagca gacggcgtag
     2641 acctgcttct tgacgcaatc gctgactatg ctcccaacgc ggcagagcac gcaggcgaaa
     2701 ccgcaacaga cgcaagcggc gcagagaaag aactcgcatg cgacgaaaac ggtccccttg
     2761 cagcagtatg ctttaagacc gttgccgacc ccttcgtcgg taagatgtca ttcattaagg
     2821 ttgtatccgg caagatgaca gccgatgttc ccgcatacaa cgcaagaaca ggcgaaagcg
     2881 aaagaatcgg caagctgctc tccgtcaagg gcggcaagca ggaggatatt tctgttctgc
     2941 ccgcaggcga tatcggcgtt gttacaaagc tttcaagcgt aatgacaggc gatacaatct
     3001 gctcgcccaa ggatgttctc catctcaagg gcgttcagtt cccgaccccc tgcctctcaa
     3061 tggctgttaa ggctgttaag aagggcgagg aggacaaggt ttcttccggc ctcagaaagc
     3121 tcatggaaga agacccgtca atcgtattct ataccaacga ggaaacaaga cagcagattc
     3181 tctcaggtct cggcgagcag caccttgatg ttatcaccac aaagctgcag gctaagttcg
     3241 gcgttaaggt tgagcttgag attccgagag tcgcatacag agagaccatc cgcaaaaagg
     3301 tcgaggctca gggcagacac aagaagcaga ccggcggtca cggtcagttc ggcgatgttt
     3361 atatcagatt cgagccgtgg gatacagagg accttgtatt tgctgaggaa atcgtcggcg
     3421 gcgcagttcc caagcagtac ttccccgccg ttgaaaaggg tctcagagag tgcgtagcaa
     3481 agggcttcct cgcaggctat ccgatggtcg gcgtaaaggc tacactctac ttcggttctt
     3541 accaccccgt tgactcatcc gaaatggcat tcaagattgc cgcttcactc gcattcaaga
     3601 acgctatgcc tcaggcttcg cccacaattc ttgagcccat cggcacactg aaggcttaca
     3661 tgcccgacga gaacctgggt gacatcatgg gcgaaatcac aaagcgccgc ggcagagttc
     3721 tcggcatggg tcccgcaaag aagccgaaga tgcaggagct tacagcagaa gttccgatgg
     3781 ccgaaatggg cgacttctcc acaactctcc gctcggtaac ccagggcaga ggctccttca
     3841 cccttgagtt cgcaagatat gaggaggctc ccgcaaacat cgttgagaag gttatcgagg
     3901 aagcaaagaa gagacaggaa gaggaagaat aaaaacaata aagagcctta tgaaaataag
     3961 gctcttttat tatttcaagc ccaccaaatg taaaaatacg gtaataaggt agaataatag
     4021 tcatattcat gaaaattgtt caaaaattgt ttacagacct tcaatttaaa cacaatcgaa
     4081 aaatattagt atatagtcag gaactccaat tccttttcat tttattttct cttcccaaag
     4141 tagtgctccg gaaaaatccg gggcattatt tttttgcgct aaaaaaccgc agggcaaaag
     4201 ctctgcggca tttctttaaa tcatcgcgtt gtctgaaagc tgtcttacat atgcctccat
     4261 ttcctgcttt gaggaaacct gagaggcgcc ttttatgaac ttttccttat cagtttcgga
     4321 aaggctagcg aattgggtta aagcgtcaat gtccttagcc aaagcggagg taagacccaa
     4381 aggcatatcc gccatgtttt ttttcttatc cttgctcata ttaaaacccc ctgaatcagt
     4441 ttcctccggg gccgtctctg cgctccctga agtcattgtg aggctgtgtg gctgcccact
     4501 gattgttgcc tgctgatttc ttgccggact ttgccttgcc ctgaatttcg ggcacgggct
     4561 ctacaacatt tttgggatat ttaattccca gttcgtccgc gccgggtctt ttcatgccct
     4621 gttttgccat aaaaacacct accttcaaaa ttataattta caaaaaaggt cggtttatgc
     4681 aaaataaaaa atggcgggtt atttgcccgc cgtttgtttt ttaattaaat gataaaattg
     4741 ccgttgtttc ggaatcaata tttactcctt ttttgtcatt cgttactgcg ccgccgatta
     4801 acgcttttgg attttttatg ttttcgggca ggtaataggt aatttcatga tggtttttat
     4861 ttgaaattac aaaaacgctc tcgccctttt cattttttct tataaatgcg acgcagccca
     4921 gcattgcgga aacggggaca aactcccctt ccttaaaggc gtcgtgtgag cttctgattt
     4981 cgccgagggc tttgaagtgg ttaaggattt cctcatcttc atttccccac ggaaagcatc
     5041 gtctgttgaa ggggtcgacg ccgccctcca agcctgcctc gtctccgtag tagatggtgg
     5101 gaactccggg cagggcgtaa ataaggcttg cggcgcaaag aaggcgtttt ttagctattg
     5161 ccttttcgct gtcagtgagg ctaaactccg cctgacgatt tttatctatg tgcgagagct
     5221 gagcgcccga caaaattgtg agtacccttg cggtgtcgtg ggtgccaagg aagttcatag
     5281 aggcgtcaag ggcgggcttt gggtagtttt cgcagattgt aacaatgcgc tccataaaaa
     5341 gctccgcgga gccgcccagc ataaagtcta tgaccgcctc tctgaagggg tagttcatga
     5401 ctccgtcaag ctcgtcgccg agaaggtatt ttcttctgcc gccgtagctg attttgttgc
     5461 ttgcgtcctc ccagacctcg ccccaaagaa gggcatcggg cttatgtttt ttaacagttt
     5521 cgcgcacctt ttcaataaag atatcgggga gttcgtcggc aacatccagt ctgaagccgt
     5581 acgcgccgtt tttcagccag tggtttacaa ctcccctgtc gcctgttatg aactctttga
     5641 agctttcctc gttttcgttt acctcgggca gggttttgac gccccaccaa gaggcgtagt
     5701 cgtctctcgt ttctccgaac ttataccatg aataatatgg ggactctggg tcgttgtatg
     5761 ccccgccgtc gccgtagcgt ttatatttat tgaagtaaac gctgtccgct cccgtatggc
     5821 tgaaaacgcc gtcaagaatg atttttatgc cgtactcctc tgccttcttt gtcagagaaa
     5881 cgaaatcctc ggcggtaccg agcagggggt cgattttaaa gtaatcggcg gtgtcgtacc
     5941 tgtgatttga gtgcgcctcg aagatagggt taagataaat cagccccacg ccaaggctcg
     6001 caaggtactc aagcttttct tctatgccct ttaagtcgcc gcagaaataa tcgttattga
     6061 atctgccgtt ttcatcggga taccatttgg gagtgccgcc ccagtctgag cgcagctctc
     6121 tgtctttcgg aacattctcc tttttctctc ccgagaagcg gaacctgtcg gggaaaattt
     6181 gataagccac ttttcctttg agccagtcgg gagtactgaa attctcatcg tagacagtca
     6241 gctgccatgg gacgccctct ctgcccacat agcctattcc cgaaccgctg tttaaaacgg
     6301 tgcccctgcc gtaaggggtg tcataatcga atttatagaa atacagtccc ttattgggag
     6361 ttttgaactc ggtgcgccag aagtggaatt cgccgttttc gctctttgtc atgggtatat
     6421 attcttcctt ttttccgtcg ggccagataa caaggaaagc cttttctgcc aataaagagc
     6481 tatggagcac aagcgtaaag cctgtgctct cgtttgtttt tattgcgccg aaaaccgact
     6541 tgtattttct gtcccgcgaa ttaaaggcga tttccactgc ctcacctgct gtcgtcgtta
     6601 acccttgacc ttaagttttc aaatccgaat gcggagggcg tgccccagat attctcggca
     6661 tactgcctta ccgctctgtc cgcggcaaag cggccggctc ctgagatatt gataagggac
     6721 attctgttcc atttttccct atcggaataa agctcgtcgg agcgtttttg agcaagtcta
     6781 tagtccgcat aatcggcaag aaccatatag gggtcgtagt ttgttatgga ctttgaaatc
     6841 tcgtcaaagc tcttgccgcc gactccgccg ctgtttataa agtcaattgt gcgctttaaa
     6901 tccttattgt taaagtagta atcggagggt ttatagccga tgtttctgag atgctttacc
     6961 tcgtcctcct taagaccgaa aatgatgatg ttttcttccc ctaccgcgtc gtaaatctcc
     7021 acattggcgc cgtccatggt gccgagggtt atggcgccgt tgagcatgag tttcatattg
     7081 cccgtgccgc tcgcctctgt tcccgcaagg gagatttgct cgctgatttc agccgcgggc
     7141 attaaaagct cagcctcggt gacggagtaa ttctctacaa agatgacctt gaggtatttg
     7201 ttcacctcgc tgtcgttgtt gataaggtcg gccaaagctg aaataaagct gataatcttc
     7261 tttgcgagaa agtacccggg tgccgccttt gcggagaata tataggttct ggggatataa
     7321 ttgccgttcg ggttttcctt tatctcaaga tacttattga ggatattcag cgcgttgaga
     7381 tgctgccgtt tgtactcgtg caggcgtttg acctgaacat cgaatatgga ttcggggtca
     7441 atctctacgc acattttttt cattatataa tcggcatatc tctccttgtt tttgagcttg
     7501 attttgttca gctcgtcaag gacggacttg tcgtcagcat atttccttaa attaataagc
     7561 tcatcgccgt tgtatacaaa gctgtcgccg ataagctcct taatgaaatt cgcaaggagc
     7621 gggtttgcct gacagagcca gcgtctgtga gctatgccgt ttgtcacatt cttaaacttt
     7681 gagggcatca gcttgtagta atcattgaaa accgtatctt tgagaatacc gctgtgcagc
     7741 tctgaaacgc cgtttacgct gtgggagccc gctatgcaga gattagccat tctgaccgct
     7801 gagtcggaaa tgacggcggt gctctccaca aggtcggggt cgtggctgct gttccataca
     7861 aaggagcgga aacgattgtc aatctccttg ataatctgat aaatcctcgg aagaagtctc
     7921 tttaaaaggt cctcgctcca gacttcaaga gcctcgcgca taacggtgtg gttggtgtaa
     7981 gctattgtgt tggtgactat tctccacgcg tcgtcccagc cgtaaccgca ttcgtcaagg
     8041 agaatgcgca taagctcggg aattgcgagg gtcggatggg tgtcgtttat gtgaattgca
     8101 accttctcgg gcatattgtc aagggtcggg tatctcctta aatgctttcg gactatatcc
     8161 tgaatagcgg cggagacaag gaaatactgc tgtctgagtc tgaggctctt gccctcgggg
     8221 tgattgtcgg caggatatag gaccttgctt ataatctccg ccatggaggc gttttccata
     8281 gctcttaaat aattgccctc attgaaaaga gccatgtcga tgccgggggc gaccgctgac
     8341 caaagtctca agacgcttat gcccttgcct cccttgccgg agacacacat atcataggga
     8401 accgccttaa tcttttggca gtcaataagc tccacatggt ggaacttttc atgccattcg
     8461 tccttaaccc agccttcaaa gagtatgtcg acggcgctct cctcccgctt tacaagccaa
     8521 gcgtcgccgc cggaaagcca gaaatcgggc agctcggtct gccagccgtc aaccagcctt
     8581 tgtttaaaaa taccgtactc atagagaatc gaatagcctg ttgcgttgta gccttgcgtc
     8641 gcaagagcgt caaggtagca ggcggcgagc cttccgagac cgccgttgcc gagacccgcg
     8701 tcgggctcga tttcgaatac tttgtcaagg ctgatgccgt agtcggacaa aacctcgtca
     8761 aaaacattta aaaggtcaag attgtataga ttattcttta aagacctgcc catgagaaat
     8821 tccatggaca gatagtatat atccttggcg tttttctctc ttgcctcttt ttcgaattcc
     8881 cgcctctgac gattcatcat ttcggtgaga atcatggcaa gggcgcggta aacatgctca
     8941 tagcttgccg actccgcgcc tacgccgaag aaatgaagca gcttgctttc aagcagctct
     9001 ttagcttttg ctttggtaag tttatattcc atgcgctcct cacaatctgc ggaaaactcc
     9061 gcggttatga ttgcaaaatc aatctgttaa gtacactgta tttacataat ataacaaaat
     9121 gcgtccctct gcaagagatt cggggctcgt gctttgtcaa aagtgttgag tttgacccgc
     9181 tttatttatg caaagtgcca aaacgctctt ttttaaagaa taaatagtga aaataaaagt
     9241 atgattattt ttagacgaca aaaaattcat tttagggaca atgtccctac tatagtattt
     9301 gcgttttttt catttaaagc agtaatttaa tcacaaatat acagtacatc cataatgagg
     9361 ggattttttt gtatattaat ttcctgttta atctatcttt ttatgaatat aataatagca
     9421 gggggtgtta ggggttgaag gtgctttcaa aatcttacga acaatactgc tgtgttcttg
     9481 aaagaaatgt tgtgatggaa gaagcaacct accacaacgg cggaattaaa cttttttgca
     9541 ccaactatcc gcagtgcttt acctgcggcg gatgcaagaa tataattctg aacgacaaaa
     9601 tagagaaagc cgctatggtg aatacaatga tctaaacctt accttaaaaa gtggttttca
     9661 taccccgcca ttggcgggtt ttgttgtttt gggctttggg cgcagcgctc aaagcatttt
     9721 tgctttacaa gaaaacctta tactgttata atgtttaagg ttaaaaagcg gctttaaaag
     9781 ggctgttaga caaaatatag ggctttttag cccgcaggct tttgaaagga aagaaattat
     9841 gaaatacaca tccactcgcg acagcagcct tcagatttct tccgccgagg cgatagtcaa
     9901 gggcatatcc cccgacggag gtctttttgt gcccaccgaa ttccccaaag tcgacggcga
     9961 ttttatagcc tctcttacaa agatgaacta caaggaaagg gcaaaggcag tgctgtcttt
    10021 gtttttgacc gatttttcgg aagatgagat aagcggcttt gttgaaaaag cttacagcga
    10081 gggcaagttt gaaagccatg aggtagcccc cttaaagata ctgcctaacg gcgtcagctt
    10141 ccttgagctg tggaagggcc cgacctgcgc gtttaaggat atggctctgc agcttctgcc
    10201 ccatctcctt acaggcagcg caaagaagat tggcgatgac agccttatag tcattctgac
    10261 cgccacatcg ggcgacacag gcaaggcggc tcttgagggc tttaaggatg ttgagggaac
    10321 gaagatactt gtcttttatc ccagcgaggg cgtaagcgac attcaaaagc tgcagatggc
    10381 aactcaggaa ggaggcaatg tgggcgtcgc ggcgatttac ggcaactttg acgatgccca
    10441 gagcggcgtt aaggatattt tcggcgacaa ggctcttgag gctgagcttc tgagcaaaaa
    10501 cataaagttc tcctccgcaa actcgattaa ctggggcaga cttgtgccgc agattgtcta
    10561 ctacttctcc gcttactgcg atttagtaaa taggggaaaa gtaaagtatc ccgaaaaaat
    10621 caacatctgc gttcccacgg gcaacttcgg caacattctt gccgcctact atgcaatgag
    10681 aatgggtctg cccgtaaaca agctgatatg cgcctccaac cgaaacaata tcctgaccga
    10741 ctttatcaaa acaggtgtat atgacaggaa cagagaattc tttaccacca cttctccctc
    10801 aatggatatc cttatttcaa gcaaccttga gcgtcttctt tatgagctga gcggcaggga
    10861 cgacgctaaa atcaaagagt ggttcacttc acttaaagaa aacggcaaat atgaagtaag
    10921 ccccgaagtc ttaaatgagc ttaaaagcgt atttgagtcg ggctactgtg gggatgagga
    10981 aaccgccgcc gaaattaaga gggtttacga tgaattcggc tatcttatag atacccacac
    11041 cgccgtcggt tcattcgttt atgaaaatta cagaaagaca tcgggtgatg acactcccgt
    11101 agtcatagct tccaccgcga gcccgtacaa gttctgctca agcgttctca gcgctcttga
    11161 gggcagagac tcatcaggcg aaaacgagtt cgaaattatg agaaggctcg aaaagctcac
    11221 aaacacagcg gctccgaagc agcttaaaaa ccttgaggga gtatcgccga gatttacgga
    11281 agtttacgac aaaaaggata tgcggcaggc agttttttca atgctgaata tctaaagttt
    11341 taagctaaag ttaattaaaa atatgtaaat ttaaaaagca agacggctgc catgcgcatt
    11401 aaagtgaatc gttttagcag cacgacagcc tcttgctatt tttattaggg gtagttagca
    11461 gttcgcgtgt ttatccagct cgaaggttat acatgcggtt tcgctgcaat cctttgcatt
    11521 acgggttcct atctctattt ctcccgccgt gcacctctgt tcgggcgtct ggtaaacaca
    11581 gttcttaact tcgcacttga tattgtttat tacatgtctc ttctcgccca tttttacggc
    11641 tcctttcgcc tgtccaatga cagccgaaac cccaaacttc atatccaatc cggcaacagc
    11701 cttcacatca tatcggatgt gatccgtcgg attcggctgt ctttttggac attaatatta
    11761 tcgacctatc gtttttttat atcccttgaa aatatatctt tttaacttca aaaaagcgtt
    11821 aaaacggaga aaaagatgtc tgtttttgca atcggcgaca cacatttatc ctttggtact
    11881 gacaagccca tggatgtttt caggggctgg acttcttata ccgaaaaact gagagaaaac
    11941 tggataaagt ctgtcggcga aaacgacacg gttgtaattc ccggcgacat ctcatgggcg
    12001 atgcgccttg aagaaagcga aaaggatttt gagtttcttc attctttacc cggggaaaaa
    12061 ataatactca agggcaacca tgattattgg tggagcacaa agggaaaaat ggacgctttt
    12121 ctctcaagca aaggtttcca ttccataaaa attcttcata acaacgctta caaagtcgga
    12181 gatttcgcaa tttgcggcac aagaggctgg ttttttgacg atgacgagcc gcacagcgaa
    12241 aaaattattt taagagaggc tatgaggctg aaaacctctt tggaatgcgc gatgaagctt
    12301 ggcggagagc ttttggtttt tctgcattat ccgccgctga gctccacgca aaaatgcgat
    12361 gagatttaca atatccttct tcagtatccc gttaaaaggt gcttttacgg tcacattcac
    12421 gcggacatga cggaaaaata ttcctacttt tttaaagacg gaatcgaatt taaactggta
    12481 tccgccgact acttaaaatt ctccccgctt ttgattgagg aattttagca tagtaccttt
    12541 aaatttttgg gtcttgcgct atctttagat ttattataat atattttagt gttaacttag
    12601 gatttttttg ttacttctgc cgctataaat ggttttgcgg ctgtttgatt aaagactata
    12661 gcttttacca gcagtaagga gacagcaaat tatggctaaa aagaataccg aaaatcaagc
    12721 agaagtttat cgtcaaaagc gcaaagagag gctgaagaaa aaacagcaga gaatggcgaa
    12781 gcgccgtcta agccccaagg ctcagaaaat tatcggcagg acagtcagaa tcgtcgtt
//
LOCUS       DAONVQ010000048        12783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3765_length_12783_cov_4.604101, whole genome shotgun sequence.
ACCESSION   DAONVQ010000048 DAONVQ010000000
VERSION     DAONVQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12783
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3765_length_12783_cov_4.604101"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLD98_04445"
     CDS             complement(<1..336)
                     /locus_tag="PLD98_04445"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQA05532.1"
                     /translation="MKKYRRIWVEVDLDALHHNLNEAKRVSKGAKILAVVKADGYGHG
                     AVEIAKELHEEADYFGVALIDEALQLRRNGVTKPILILGYTSPTQYETLIEADITQSI
                     FSREAARQLS"
     gene            complement(388..1446)
                     /locus_tag="PLD98_04450"
     CDS             complement(388..1446)
                     /locus_tag="PLD98_04450"
                     /inference="COORDINATES: protein motif:HMM:NF025694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HQA05533.1"
                     /translation="MDKSSLSLLNFGDSLDSIANLDPRGYGVCRVLYKAARDMANEPL
                     SIKGAKLIIDSLAPKGLVYIISGFVLPDCEKAETDGLIGAVLLSKVLALYFRAKPVII
                     CPPEALSAAEAASRAIGLEVISSIPDASDRPYLSLMPFTKDKAKALDETAALLENGMP
                     CLVISIEAPGSNAEGEYHSAKGINITELEAKSDALFRLLQNMGVPSLSIGDLGNEIGL
                     STLRESVLKYIDKAGKSCQCGCVSGIFSDTAADAVITATASDWGVYALISAIALLRGN
                     KEIIPSGDELKDALRAGVENGLVDMSGKAVPAIDGFNESLNAAVLSLMRELVSHAIDN
                     RGEYEYWFDSVLQSGYFN"
     gene            1572..2699
                     /locus_tag="PLD98_04455"
     CDS             1572..2699
                     /locus_tag="PLD98_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HQA05534.1"
                     /translation="MNIYLDTSATTAVCKVAADAAYSVMTEFYGNPSSIHKKGIEARN
                     ILENSRESVASLLGVSPEEIYFTHGGTESNNTAIFGAVNKGKRYGNRIVTTSVEHPSV
                     KEPMKVLEAQGYEVIWLKADKYGDFSVEELYNAVDKRTILVSIMAVNNEVGTILKTEQ
                     AAAAIRAAGAPALLHVDGVQAFGKLKINPSRFGIDLMSMSAHKLHGPKGVGALYIKKG
                     TGILPISFGGGQERAMFPGTEAMPAIAGFGAAVKACGDIEHNFYKVANLNGILRSGLG
                     SIRDVYINSPANALPYILNISVKGIPSQVLVNFLSERGIYISAGSACKKGKRSEVLTA
                     MGLPPALIDSAVRISLSRFNTENEMYATLNAIEDAVKRLRR"
     gene            2880..4058
                     /gene="thiI"
                     /locus_tag="PLD98_04460"
     CDS             2880..4058
                     /gene="thiI"
                     /locus_tag="PLD98_04460"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HQA05535.1"
                     /translation="MRETILIKNGEIALKGLNRKNFEDALVKNIRDRIYDLGEFKYIK
                     SQSTVMVEPLSPGIDLDEAFERLKKVFGIAALSRAVACEKDMNEILHYTCVFLKDSLM
                     AAKTFKVEAKRSDKKFPLKSPEISAETGAKILEEFPHLTVDVHNPDIIVMVEIRDREA
                     FIHGEQVKGAGGIPVGTGGKAMLLISGGIDSPVAGYMIAKRGISITAVHFASPPYTSE
                     LAEQKVHRLLKKVSEYSGRIKLYTVPFTELQEAIRAHCPEELFTLVMRRLMMKISCVL
                     AEKEDCKALVTGESLGQVASQTLGALQCTDDASVLPVLRPLIGMDKDEIVTISRKIET
                     FDISIEPFEDCCTVFTPRHPRLKPDLKRVIQAEEKVLSAFPEDILIKTAEASKVLEIG
                     "
     gene            4162..4236
                     /locus_tag="PLD98_04465"
     tRNA            4162..4236
                     /locus_tag="PLD98_04465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4195..4197,aa:Arg,seq:cct)
     gene            4404..5252
                     /locus_tag="PLD98_04470"
     CDS             4404..5252
                     /locus_tag="PLD98_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05536.1"
                     /translation="MKKWVSFCLALVLLFSAVYVGAQAGMSDSPESMSILLTEGCILL
                     KNSAKLSDLMFLDLDENYVWSGITISPYEWFDLKAAAKSLKIKAEFNGRTLTFDDYLP
                     TGAVIAIETPEGEEKKYTAVLYGDVNCDGKITAADARFLLRVAAKLDTIDDVYIYEAA
                     KLTNGLVVDAEDARRVLRTAALLDDNLRVMYNKTAYYNWKLPDIEVIFHPGKDGGADE
                     LEKFEFVESVSAADSILIEGTEYPCYKLAIKEPYIDNFMRLRFECRGDINIYDTRNVW
                     AFGMPA"
     gene            complement(5326..6618)
                     /gene="mtaB"
                     /locus_tag="PLD98_04475"
     CDS             complement(5326..6618)
                     /gene="mtaB"
                     /locus_tag="PLD98_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HQA05537.1"
                     /translation="MKAYFYTLGCKVNQYETQEISETLSANGIFRASSPEDADIIVVN
                     SCTVTQESTRKTKQAVRRFKKQNPNAVLVLTGCLPQAFPDEAEALEEIDVLLGNKNNH
                     DLLTAVNYYLQTGARVNNVQLHEIKKGDEYKGVGISSLDEHTRAYLKIQDGCNRFCSY
                     CIIPTARGRSRSKPLEEIENELRRLAQNGYKEIVFVGINLSAYGREMGLSLADAVETA
                     EKEEGIERIRLGSLEPDHITDELLERLSRIRKLCPHFHLSLQSGSNSVLKRMNRHYTA
                     EKYLEIMDMVRRHFPDAAFTTDVIAGFPGETDEEFAETAEFLKKARFQKVHIFPFSPR
                     KGTRAYDMEGQIPKNIKHSRCAFLNEICAEIRTGILKAQIGKTFEVLFESAEKGKAGG
                     YTRTYIPVEIISEENLQGQLKNVLITGVENDCCTGKLV"
     gene            complement(6680..8134)
                     /locus_tag="PLD98_04480"
     CDS             complement(6680..8134)
                     /locus_tag="PLD98_04480"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HQA05538.1"
                     /translation="MSLLSTVEKVLTRSLMKSGKKGNAKNPAPPLNDSYVSIVKEAKS
                     EYFSVGFGKQEIMPPEGVTGKKKYYVAGYRVNNPATGVLDPMMAKAIWIDDQSGAGGV
                     VFCSVDCVGLFKYDVDLVKESLKDFVKETGCRSINICSTHNHAGIDTMGLWGPLPRSG
                     RDKEFMQIVRNGIKKAIELAYADRREGSLYLGYGRADEGMQRDDRLPRVFSDKLTRIR
                     FVPNDGSREIYFVNFASHSESLEGKNSLVSADFPAYIAEEIMAQKGAETVYFVGAIGG
                     LVRMWPMDDDNVISTKKTGKRLGEIVCDIKDEVKLEPKISLAKREFYVEAENSVLIAA
                     SKILKIIPSKVYYIGAGKYGMGLKSEMTYMEIGSLKMLLIPGEIFPELVYGGYLSAEE
                     SAEGKGPEINPKPLCEIAGDENLLVFGLANDEIGYIVTPNDFYLHPEIPYLDKTQDRL
                     GRRHYEETNSLGPNTAYKIAEMFEKLLKDIKNAE"
     gene            8415..8499
                     /locus_tag="PLD98_04485"
     tRNA            8415..8499
                     /locus_tag="PLD98_04485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8449..8451,aa:Leu,seq:gag)
     gene            9058..9639
                     /locus_tag="PLD98_04490"
     CDS             9058..9639
                     /locus_tag="PLD98_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HQA05539.1"
                     /translation="MDVIKVSKRDFAVKAKQLRRDGKIPGSVFGGPLSDSISLQIDED
                     IARRLIRHKREGSKLKLDLDGQLIPVQIKEIELNTIKNEILHISFQALKADQKVKSVI
                     HIILKNTEKITGLLETMLLEIPYAALPENMIDTITIDLDGIKVGTVITVADIPELMSD
                     KIDLQVDPNEIVLRVSDKRRSVEQPAEQADEEE"
     gene            9778..10026
                     /locus_tag="PLD98_04495"
     CDS             9778..10026
                     /locus_tag="PLD98_04495"
                     /inference="COORDINATES: protein motif:HMM:NF018016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HQA05540.1"
                     /translation="MTQLAIIKDKIKKLYETNPNIHINVFKKRPNIRLINEPAVIKGI
                     YPHIFLIEERSSGFSRRRSIQYADVLTKCVEIIEMAAE"
     gene            10421..10867
                     /locus_tag="PLD98_04500"
     CDS             10421..10867
                     /locus_tag="PLD98_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQA05541.1"
                     /translation="MASIMRCISVISRCAAQYRTNKLEGTGLTGNQYAYILNVCRHPG
                     ISQDGLARMIYINKSNVARQLAKLEENGYVERRISANDKRIIEVYPTDKAFEILPRVQ
                     EVLRDWNNYLTAEFTPEECELLTRLLEIAMRRATDYVNSRDEKSEI"
     gene            10891..12603
                     /locus_tag="PLD98_04505"
     CDS             10891..12603
                     /locus_tag="PLD98_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05542.1"
                     /translation="MKKIIHYLRPYIPLMCLGLTIKFIGTIMDLALPWILSYIIDDVV
                     PRGSKTGIFLWGGAMILCAAVSIVGNISANRIAAKVSRNTTERIRHDLFARTMRLSCS
                     QSDRFTLPSLISRLTSDTYNVHHMFSMMQRRGVRAPILLVGGICVTITLEPVLTLVLV
                     AVLPLLALVVWLVSRHVIPLYTLVQNAIDKIVRKVQENITGVRVIKALSKSDYERARF
                     DEINKDAVKREQRAGMVTNITSPVMNLLLNGGLTAVIVVGAFRVNAGLTQPGKIIAFL
                     SYFTIILNAMMMVTRIFVNLSKGTASARRIAEVLEAEPDLAVTERNHVNNGYHIEFDN
                     VTFSYNKTEPNISGLSFGLKRGQTLGIIGATGSGKTTIVSLLMRFYDPDSGVVRIDGD
                     DIRGIPDDELRSRFGVVLQNDFLMADTILENIRFGRDISDEDIIRAAEVAQADAFIRE
                     KEDGYNYVLTGMGTNLSGGQKQRLLIARALAGNPEILILDDSSSALDYKTDAALRSAL
                     RRSCEGVTKVIIAQRVSSIRSADLIIMLDDGQVIGMGTHSELMKNCESYREIAEAQMG
                     DGME"
     gene            12600..>12783
                     /locus_tag="PLD98_04510"
     CDS             12600..>12783
                     /locus_tag="PLD98_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05543.1"
                     /translation="MNKNSKFDTASIRRAVDAQKGVKAPKKYLIGRLWKYLYHYKWLL
                     LLAAVLTISSNLFSLVG"
BASE COUNT         3383 a         2928 c         3097 g         3375 t
ORIGIN      
        1 ggaaagctgc cgggcagctt ctcttgaaaa aatcgactga gttatgtctg cctcaattaa
       61 ggtttcgtac tgggtcggcg aggtgtagcc gagaatcagt atcggctttg taaccccgtt
      121 gcgccttagc tgcagcgcct cgtcgattaa ggctacgccg aaataatccg cttcttcgtg
      181 aagctccttc gctatttcaa ccgctccgtg accgtagccg tctgccttta ctaccgcaag
      241 gattttcgca ccctttgata ctctttttgc ttcgttcaga ttgtggtgca aggcgtccaa
      301 atcgacttct acccagatac gcctgtattt cttcattaca ttatctccct tgagtttgaa
      361 tttgccatag ggcagatatt ttgaagtcta attaaaataa ccgctctgta gcactgagtc
      421 gaaccagtat tcgtactctc cccgattgtc tatggcgtgg ctgacaagct cccgcataag
      481 actaagaacc gcagcattaa ggctctcgtt aaagccgtct atggcgggca cggctttgcc
      541 gctcatgtca acaagaccgt tttcgacgcc cgctctcaag gcatctttaa gttcatcgcc
      601 agaagggata atctctttat tgcctctaag caaagctatg gcggaaataa gggcgtacac
      661 gccccagtcg gatgcggtgg cggtgattac agcgtctgcc gcggtatcgg agaaaattcc
      721 gctcacgcag ccgcactggc agctctttcc agctttgtct atgtatttta agacgctttc
      781 cctcaaagta gaaaggccaa tttcgttgcc caaatctccg attgaaaggc tgggaactcc
      841 catgttttga aggagcctaa aaagcgcatc gctctttgcc tcaagctccg taatatttat
      901 gccctttgcg ctgtggtact cgccctcggc gttagatccc ggagcctcta tgctgatgac
      961 aaggcagggc atgccgttct ccaaaagggc tgccgtctca tcgagcgctt tagccttatc
     1021 ttttgtgaag ggcataaggc ttaaataggg acggtcggat gcgtcgggta ttgaggaaat
     1081 gacctcaagc cctattgccc ttgaggctgc ctcggcggcg ctcaaagcct caggcggaca
     1141 aataataaca ggctttgccc tgaaataaag ggccaaaacc tttgacaaaa gcactgctcc
     1201 tattagtccg tctgtttccg ccttttcgca gtcgggaaga acaaaaccgg atataatata
     1261 tactaaacct ttgggggcaa gactgtcaat aatcagcttg gcccctttaa tgctcaaagg
     1321 ctcatttgcc atgtctctcg ccgctttgta aagaacacgg cagaccccgt agcctctggg
     1381 gtccagattt gctatactgt caaggctgtc accgaaattt aacagactca gggagctttt
     1441 gtccatcaaa tcaccttttt tataagaatt tgtccgttaa ttgcacaatt caatctgcgg
     1501 cagtataata taattaatgg cgcctaattt tatttaagcg ttcatcaacc agcttaatgg
     1561 agggataacc tttgaacata tatcttgata ccagcgcgac gacggctgta tgcaaggtcg
     1621 cagcagatgc ggcgtactcc gtcatgaccg aattctacgg caacccctca tcaattcata
     1681 agaagggaat cgaagcaagg aatatacttg aaaactcaag ggaaagcgtc gcttctctct
     1741 tgggagtcag ccccgaggag atttacttta cccacggcgg caccgaatcc aacaacaccg
     1801 cgattttcgg cgcggtgaac aagggcaaga gatacggcaa cagaatagta accacttccg
     1861 tagagcaccc cagcgttaag gagccgatga aggttctgga ggctcagggg tacgaggtta
     1921 tatggctcaa agccgacaaa tacggcgact tcagtgttga ggagctttat aacgcggtgg
     1981 acaaaagaac tatcttagtc agcattatgg cagtaaacaa cgaggtcgga acaatactta
     2041 aaaccgaaca ggcagccgcc gctataagag cagcgggcgc tcccgctctc cttcatgttg
     2101 acggagttca ggctttcgga aagctgaaaa taaatccttc tcgtttcgga atcgacctta
     2161 tgagcatgag cgcgcacaag ctgcacggac ccaagggcgt cggcgctttg tatataaaga
     2221 aaggcacggg tattctgccc ataagttttg gcggcggtca ggaaagggcc atgttccccg
     2281 gaacagaggc aatgcccgcc atcgcgggtt tcggagccgc cgtaaaggca tgcggcgata
     2341 ttgagcacaa tttctacaaa gtcgcaaacc ttaacggcat tctaaggtcg ggtctcggca
     2401 gcataaggga tgtttacatc aattcccccg caaacgctct gccttatatt ctcaatatct
     2461 cggtcaaggg cataccgtcc caggtgctag taaacttcct ttccgagaga ggcatttata
     2521 tttccgcagg ctcagcctgc aaaaagggca aaaggagcga agtcctcacc gcgatgggtc
     2581 tgccgcccgc gctgattgac agcgctgtga gaataagtct ttcacgcttt aatacagaaa
     2641 atgaaatgta cgcaactttg aacgccatag aggacgctgt aaagcgactc agacgctgaa
     2701 ttcagcagcc tgctttaaag caggcttttt tattgcttga aactttcttc aattaattgt
     2761 atattattct tgctgttttt gtctgcccct taatacttat tagcagaagg cgcaaaataa
     2821 aacgggcaga aaaataaagg acgggagttt tatcccgcca aacgaatcgg agtgctgaaa
     2881 tgcgtgaaac aattcttata aaaaacggtg aaatcgcttt aaagggactt aaccgcaaga
     2941 attttgagga cgcccttgtt aagaatataa gggacagaat ctatgatttg ggagagttta
     3001 agtatattaa gtcccagtca actgttatgg ttgagccttt aagcccggga attgatttgg
     3061 acgaggcttt cgaaaggctt aaaaaggtat tcggcattgc cgctctttca agggctgttg
     3121 cctgcgaaaa ggacatgaac gagatactgc actacacctg cgtgttttta aaggacagcc
     3181 ttatggcggc aaagaccttt aaggttgagg caaagaggtc cgataagaaa ttccccctta
     3241 aatcccccga aatctccgcg gaaacaggcg caaagatact tgaggagttc ccccatctga
     3301 ccgtggatgt ccacaacccc gacattattg tcatggtgga gataagggac agggaggcgt
     3361 ttattcacgg cgagcaggtc aagggcgcgg gcggcatacc cgtcggcacc ggcggaaagg
     3421 ctatgctttt gatttccggc ggaatcgaca gccccgtggc gggctatatg attgccaaga
     3481 ggggcataag cataaccgcc gttcattttg cctcgcctcc ttacacctct gagctggcgg
     3541 agcagaaggt ccacagactt ttaaagaagg tcagcgaata cagcggcaga attaagctct
     3601 acactgtacc ctttacggag ctgcaggaag caataagagc ccactgcccc gaggagcttt
     3661 ttacccttgt aatgaggcgg cttatgatga aaatctcctg cgtacttgcc gaaaaagagg
     3721 actgcaaggc tcttgttacg ggcgaaagtc tgggtcaggt agcaagtcag actctcggag
     3781 ctttgcagtg caccgatgac gcctccgtct tgcccgttct aagacccctt ataggcatgg
     3841 acaaggacga gattgtgacc atctcaagga aaatcgaaac ctttgacatt tcaatagagc
     3901 cctttgaaga ctgctgcaca gttttcactc caagacatcc gagattaaag cccgacctta
     3961 aaagggttat tcaggctgag gagaaggttc tcagcgcttt cccagaggat atactcataa
     4021 aaaccgcgga agcctcaaag gttttggaaa taggctgata caaaaaaccc gccgcttaaa
     4081 cggcgggctt ttaatttaag aatggaagga ttttagattg acaattataa agttttttga
     4141 tacaatcctt tttgcagaaa tgccctcata gttaaatgga tataacggtc ccctcctaag
     4201 ggacagttat aggttcgatt cctattgagg gtgccacagc cgatggacct tggttcatcg
     4261 gcttttgttt ttcctcagaa aatattaacg aagctttgca aaactttaaa attttagctt
     4321 tcaggaacaa tttgccaatg tatcgcatca tatttataac acgcgtttat ggcacgcggc
     4381 aaataagaaa ggaagtgtta ggaatgaaga agtgggtatc gttttgtttg gctctcgttt
     4441 tgcttttttc cgcagtctat gtaggcgcgc aggcgggaat gtccgacagt cccgagagca
     4501 tgagcattct gctgactgaa ggctgtattc tgttaaaaaa cagcgcaaag ctctctgatt
     4561 tgatgttcct ggaccttgac gaaaattatg tctggtcggg aataacgatt tcgccctatg
     4621 agtggtttga cttaaaagcc gccgcaaaaa gcttaaaaat caaggccgag tttaacggga
     4681 gaacgctgac ttttgacgat tatctgccta cgggcgcggt cattgcaatc gaaacgcccg
     4741 agggagaaga aaaaaagtac accgcagttc tctacggcga tgtgaactgt gacggaaaga
     4801 ttaccgcggc ggacgcaagg tttttactgc gagtcgccgc aaagcttgat accatcgatg
     4861 atgtttatat ttatgaggcc gcgaaattaa ccaacggtct cgtcgtggac gctgaagacg
     4921 caaggcgtgt tttgagaaca gccgcccttc ttgacgataa tctgagagta atgtacaaca
     4981 aaacagccta ttacaactgg aaactcccgg atattgaggt tattttccat cccggaaaag
     5041 acggcggcgc tgatgagctt gaaaagtttg agtttgtcga aagtgtaagc gccgcggaca
     5101 gtattttaat tgagggcacg gaatacccct gttacaagtt ggcaattaaa gagccgtata
     5161 ttgataattt catgcgtctt agattcgaat gcaggggcga cattaatatt tacgatacgc
     5221 gaaatgtatg ggcgttcggt atgcctgctt agcgaggctg atagaaataa aaacctcctt
     5281 ttcctgcaaa aagaccgtca atctgcccga ttgacggtct ttaaattata ccagctttcc
     5341 tgtgcagcag tcgttttcca cgcccgttat caggacattt ttaagctgtc cctgaaggtt
     5401 ttcctcggaa attatctcaa cggggatata ggttcttgtg tagccgcccg cttttccctt
     5461 ttcggcggac tcaaaaagca cctcaaaagt cttgccgatt tgcgctttca gaatacctgt
     5521 tctaatctca gcgcagattt cgtttaagaa ggcgcagcgg ctgtgcttta tattctttgg
     5581 aatctgccct tccatgtcat acgcccttgt gccctttctg ggggagaagg ggaagatatg
     5641 caccttttga aagcgtgcct tttttaaaaa ttctgcggtt tcggcgaatt cctcgtcggt
     5701 ctcgccgggg aagcccgcga ttacatcggt tgtaaaggcg gcgtcgggga aatgtcggcg
     5761 caccatgtcc atgatttcaa ggtatttctc cgccgtgtag tggcggttca tgcgctttag
     5821 aacggaattg ctgccgcttt gaagggataa gtgaaaatgg gggcaaagtt tccttattct
     5881 gctgagcctt tccagaagct cgtcggtgat atggtcgggc tcaagggagc cgagtcttat
     5941 cctctctatg ccctcctctt tttccgccgt ttcaaccgcg tcggcaaggg aaagccccat
     6001 ttctctgccg taagctgaga gatttatgcc cacaaagaca atttccttat agccgttctg
     6061 ggcaaggcgg cggagctcgt tctcaatctc ctccagcggc tttgagcggg agcgtcccct
     6121 tgcggtggga ataatgcagt aggaacagaa cctgttgcag ccgtcctgga ttttcaaata
     6181 cgcccttgtg tgctcatcaa ggcttgaaat gccgacgccc ttatactcat cgcccttttt
     6241 tatctcatgg agctgcacat tgtttaccct cgcgcctgtt tggagataat aattcaccgc
     6301 cgttaaaagg tcatgattgt ttttgttgcc gaggagtaca tcaatttctt caagagcctc
     6361 agcctcatcg ggaaaagcct gaggcaggca gcccgtcaga acgaggacag cgttgggatt
     6421 ttgcttttta aaccttctta ccgcctgctt ggttttgcgg gtgctctcct gagtgacagt
     6481 gcaggaattt acgacaataa tgtccgcgtc ctcaggggaa cttgccctga agatgccgtt
     6541 tgcagaaagg gtttcggata tttcctgcgt ttcgtattga ttgactttgc agccgagagt
     6601 gtaaaaatat gccttcatag attaccttct aagcggattt ttgagccgta cagtaatatc
     6661 ctgtacggct cttttcaaat cattcagcgt ttttaatatc ctttaagagc ttttcgaaca
     6721 tctcggcaat tttgtatgcc gtattgggtc cgagggagtt ggtctcctca tagtgtctgc
     6781 gtcccagtct gtcttgagtc ttatcgagat acggaatttc gggatgcaga tagaagtcgt
     6841 tgggggttac gatatagccg atttcatcgt tagcgagtcc gaatacaagc agattctcgt
     6901 cgcccgcaat ttcgcaaagg ggcttcgggt taatttcggg acccttgccc tccgcgcttt
     6961 cctctgcgct gaggtatccg ccgtagacaa gctcggggaa tatctcgccg gggataagca
     7021 gcattttaag cgagccgatt tccatatagg tcatttcgga cttaagaccc atgccgtact
     7081 tgccagcgcc tatgtaataa acctttgagg ggataatttt aaggattttg gatgcggcta
     7141 tgaggacgga gttttcggcc tccacataga actcacgctt tgccaggctg attttgggct
     7201 cgagcttaac ttcgtccttt atatcgcaga caatttcgcc aagcctcttg cctgttttct
     7261 ttgtggagat gacattgtca tcatccatgg gccacatacg aacaagaccg ccgattgcgc
     7321 cgacgaaata gaccgtttcc gctccctttt gagccatgat ttcctcagct atgtatgcgg
     7381 ggaagtcggc gctgacaagg gagttcttgc cctcaaggga ctcggagtgg gacgcgaagt
     7441 ttacaaagta aatctccctt gagccgtcgt tggggacaaa gcggattctt gtcagcttat
     7501 cggagaaaac tctggggagc ctgtcgtccc tctgcattcc ctcgtcagct ctgccgtagc
     7561 cgagataaag ggagccttct ctcctgtcgg cgtatgccag ttcgattgcc ttcttgatgc
     7621 cgtttctgac aatctgcatg aactctttgt ctctgccgct tctgggcagg ggaccccaaa
     7681 gacccatggt gtcgatgccg gcgtggttgt gagtggagca gatatttatg cttctgcagc
     7741 ctgtttcctt tacaaagtcc tttaaggatt cttttacaag gtcaacatcg tatttgaaca
     7801 gtcccacgca gtcaacggag cagaatacga ctccgcctgc gcccgactgg tcgtcaatcc
     7861 atatagcctt tgccatcatg gggtcaagta cgccggttgc ggggttgtta acgcggtagc
     7921 cggcaacata atacttcttt ttgcccgtaa ctccttcggg gggcataatc tcctgcttgc
     7981 cgaatcctac tgaaaagtat tcgctctttg cttccttaac aatgctgaca tagctgtcat
     8041 ttaaaggcgg ggcgggattc tttgcgttgc cctttttgcc cgacttcata aggcttctgg
     8101 tcagcacttt ttcaaccgta cttaataaac tcatctttat tccaccttct taggcacaat
     8161 tatccataga aactcattta ataatatcaa aaaagaaatg tctttgcaag gattgaggga
     8221 aatttgagga ttcatatttt gcattaaaaa cataatatga atttgattct tgctttttag
     8281 cttttaaagt tatataatat gctctgcagc ggccccggag ataaaatact ttaacatgaa
     8341 aatatgttaa atttttttag cataaaaggt attgacaaac actatggggc tttgatataa
     8401 tcgaactcgt ccgagcgaga gtggcggaat tggcagacgc gcacgtttga ggggcgtgtg
     8461 gtaaatcccg tacgggttca agtcccgtct ctcgcaccaa accccgaagc acaaaagtgc
     8521 ttcggggttt ttcatttttt cggcttttca ttggttttga agatgacatt ttacacaggt
     8581 taagggaatt catatctttt tggcaggaaa atcaaggcga aagcaactgc aaaataattt
     8641 ttatgggcac tcacagggcg cttttgtgcc gagctgcggt tttatgtgta tatccgttag
     8701 tccgttccgt taggtttaac tatatatgtg ctttgcggaa tatttcgttg aaaagcatgt
     8761 cctgccgtga tttttgtcag tttattaaac gggatagtat gttaaataat ttctaaaaag
     8821 ggggccgccc gctaagcgta aaaagctgaa aaacaggccg atttgctttt attttttttc
     8881 tgtggtacac tatatataaa tcgaatagca aaagaaccgt ctgtcagaag ggcttttagt
     8941 attgcactgg aaaggtgtta tatgaagaag tccttctttt ttaaaatcca tggcgtggtc
     9001 aacagtccta tactttaatg tgtaggctgt ataaaaacat ttaaggagga agcgcaaatg
     9061 gatgttatta aagtaagcaa gagggatttt gcagtcaaag caaagcaatt aagacgcgac
     9121 ggcaagatac ccggcagtgt tttcggaggt ccgctttcgg attccatttc tcttcagatt
     9181 gacgaggaca ttgctcgcag actgattcgt cataaacggg aaggaagcaa attaaaactt
     9241 gatcttgacg gacaacttat tcctgttcaa attaaggaaa tagaattaaa cacaattaaa
     9301 aatgaaatcc ttcacattag tttccaggcg ctgaaggcgg atcaaaaagt caagagcgta
     9361 atccatatta ttctgaaaaa cacagaaaaa attacaggct tattggaaac tatgctgtta
     9421 gaaattcctt atgcagcgtt gcctgaaaac atgattgaca caattacaat agaccttgac
     9481 ggcattaagg ttgggactgt cataactgtt gcggatattc cggaacttat gagtgataaa
     9541 attgatttgc aggttgaccc gaatgagatt gttctgcgtg tcagcgataa aagacgctct
     9601 gttgagcagc cggctgagca agcggatgaa gaagaataaa tctttgcaaa cagttcatca
     9661 gtacaaagca agagcttgca tcgcctctct gttacaggag gtatttcgga atgcgttata
     9721 caatctcaaa gccgcctgac attcacacgg cttaaactaa aaagcaggag gtaatgcatg
     9781 actcaattag ccataattaa agataaaata aagaagttgt atgaaaccaa cccaaatatt
     9841 catattaatg tttttaagaa acgacccaac atccgtttaa tcaacgaacc cgctgttatt
     9901 aaaggcatct atccgcatat ctttctgatt gaagaacgaa gcagcggctt ttccaggcga
     9961 cgctccatcc aatatgccga tgtcttaaca aaatgcgttg aaataattga aatggcagca
    10021 gaataaccgt ataaagaaga agttatgcct aaagagtatg taacgggtca tcaagcgaat
    10081 taggttccat catagagcgc ctggcatgag caacggaata catgagcatt atctataaat
    10141 atgactttaa gatgtaagta caaagcaaag cctcggttat aaaccgaggc tttatctttt
    10201 agctttcaaa aggggtcggc ggccttgttt tcagtcaggc ggttttatgg acaaaaaact
    10261 gaactcccct tttccgcaat gcaattccct atttatcata tcccgcttca ggacacgaga
    10321 gctttcatag cgcttattac aataattgac aaacgcctga agaactgcta ttcttataaa
    10381 tagttgcgct cgcaacaata ggtattcagg aggcacgaaa atggcatcga ttatgaggtg
    10441 cataagcgta atctctcgat gcgcggcaca gtaccgcaca aataagcttg agggtacagg
    10501 actgaccgga aatcagtatg cctacatact gaatgtatgc agacacccgg ggatatcgca
    10561 ggatggtctg gctcggatga tttatataaa caagagcaat gtcgctcgcc agctcgcaaa
    10621 gcttgaggaa aacggctatg tggagaggcg gatatccgcg aatgacaagc gcataatcga
    10681 agtctacccg acagacaaag cattcgaaat actgccgcga gtgcaggagg tacttcgcga
    10741 ctggaataat tatttaacgg cggaattcac tccagaagag tgtgagctat taacgcggct
    10801 tttagaaata gcaatgcgac gggcgacgga ttatgtaaac tcccgagacg aaaaatcgga
    10861 aatctgaaag aaagttcggt aataacactt atgaaaaaaa taattcatta tctgcgcccg
    10921 tatatacctt taatgtgtct cgggttgaca attaaattca ttggcacgat tatggacctt
    10981 gcgctcccct ggatactttc atacataatc gacgatgtgg ttccgagggg cagcaaaacc
    11041 ggaatatttc tttggggcgg tgccatgata ctttgcgccg ccgtctcgat agttggcaat
    11101 atatcggcga accgtatagc ggcaaaggtt tcccgcaaca ccaccgagcg catacggcac
    11161 gaccttttcg cccgcaccat gcggctgagc tgttctcaga gcgaccgatt tacgctgccg
    11221 tcgcttatct cccgactgac ttccgataca tataatgttc accatatgtt cagtatgatg
    11281 cagcgccgtg gggtacgcgc tccaattctg ctggttggcg gaatatgcgt gaccatcacg
    11341 ctggaaccgg tgttaactct tgtactggtg gcagttctgc cgctgctcgc gcttgtcgta
    11401 tggcttgtat cccgccatgt aatacctctt tacacattgg tgcagaatgc catcgacaaa
    11461 atagttcgca aggtacagga gaatataacc ggagtgcggg taataaaggc gctgtccaag
    11521 tccgactacg agcgcgcacg cttcgatgag attaataaag acgcggtaaa gcgcgaacaa
    11581 agagcaggta tggtaacgaa tatcacgagt cccgtgatga atcttctgct caacggcggg
    11641 cttactgcgg tgattgtggt cggcgcgttc agagtcaatg caggtcttac ccagcccggc
    11701 aaaatcatcg ccttcctgag ctatttcacg ataatactca acgcgatgat gatggtcaca
    11761 cgaattttcg tcaatctctc gaaaggcacg gcttccgccc gtcgcatagc cgaggtgctg
    11821 gaggctgagc ctgatctcgc agtgaccgaa cgaaaccatg taaacaacgg ttatcatata
    11881 gaatttgata atgtaacctt ttcatacaac aaaaccgagc ctaatatatc gggactcagc
    11941 ttcggactaa agcgcggtca gaccctcggc atcatcgggg caacaggaag cggaaagaca
    12001 acgattgtca gtctgctgat gcgcttctat gaccctgatt ccggcgtcgt gaggatagac
    12061 ggcgacgata ttcgaggcat tcccgatgat gagcttcgct cccgtttcgg cgtggtgctg
    12121 cagaacgatt ttctgatggc cgacacgatt ttggaaaaca tacgcttcgg tcgtgatatt
    12181 tccgatgagg atattataag agcggccgag gtggcgcaag cggatgcctt tattcgcgaa
    12241 aaagaggacg gctacaatta tgtgctgacc ggcatgggta ccaacctcag cggcgggcag
    12301 aaacagcgct tgctaatcgc gcgcgctctc gcggggaatc ctgagatact gattcttgac
    12361 gactcatcga gcgcactcga ttacaaaacc gacgccgcgc tgcgttccgc tctaagaaga
    12421 agctgtgagg gcgtgacaaa ggtcattatc gcccagcggg taagttcgat acgctcagca
    12481 gacctaatca taatgctgga cgatggtcag gttatcggaa tggggactca ttctgagctg
    12541 atgaagaatt gtgagagcta ccgcgagatt gccgaggctc agatgggaga tggtatggaa
    12601 tgaacaaaaa ctctaaattt gataccgctt ctataagacg cgcagtagac gctcagaaag
    12661 gtgtcaaagc accgaaaaag tatttaatcg gcaggctgtg gaaatatctt tatcactata
    12721 aatggctcct gctgctggcg gctgtgctta ccatctcaag caatttattt tcccttgtcg
    12781 gac
//
LOCUS       DAONVQ010000049        12666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3807_length_12666_cov_4.370946, whole genome shotgun sequence.
ACCESSION   DAONVQ010000049 DAONVQ010000000
VERSION     DAONVQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12666
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3807_length_12666_cov_4.370946"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /gene="asnB"
                     /locus_tag="PLD98_04515"
     CDS             complement(<1..854)
                     /gene="asnB"
                     /locus_tag="PLD98_04515"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQA05544.1"
                     /translation="MCGFVGFLNDTDDAELNMSIVRSMADKIAHRGPDDEDYYIDDKI
                     SMGFRRLSIIDLEGGKQPILNEDGTKVLVYNGEIYNFKDIRKELIEKGHVFATNSDSE
                     VILHGYEEYGEKVCEKLRGMFAFAIWDKKEKTLFGARDIFGIKPYFYYKNGDAFFFAS
                     EIKAFLPHPGFKKVFNKERLPEYLCFEYIPSEQTFFKNVFKLPPSSYYILKDGNLTIQ
                     KYFEMEYRIDDEKSIEYFEELISETLSKSVDIHTISDVEVGCFLSGGIDSSYVTAEVA
                     KRGKVKTFS"
     gene            complement(876..1592)
                     /locus_tag="PLD98_04520"
     CDS             complement(876..1592)
                     /locus_tag="PLD98_04520"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HQA05545.1"
                     /translation="MKEKTLGVLGGVGPLATVYFAKLLVEMTKADCDQSHISSVILNH
                     ASIPDRTEYILDNSKPNPLPVMIEDAKKLEKMGVDFIVIPCNTAHYFYEQIQSNVSIP
                     LVNIIEETVDYAQKTVKNLRKLGILATKGTITAKSYQRACEKRDIEPAVPSIQDEQSL
                     MHIIYNQVKAGKRVDLQLFLNIIETMKNDGCDAVVLGCTELSVIKHEFNIKRPDVIDS
                     LEVLAKRSIELCGKELAVEM"
     gene            complement(1630..2847)
                     /locus_tag="PLD98_04525"
     CDS             complement(1630..2847)
                     /locus_tag="PLD98_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05546.1"
                     /translation="MKFNETEFLPVILGADITAYSHARSFHEEYGIKSLVLSMLKSEF
                     IARSKIIENRVIPGLEKEEVLIDTLLKVGEEFKGRKKLIVLGCGDWYVRILIENKEVL
                     KPYYIIPYIDEELLNRIVLKDKFYAICDELDIPHPKTYVYNCGEENNLKFDFDYPVIA
                     KPANSALYHYAKFEGKKKVFRFQDEKSLREMLKKAEDAGYNYKFLIQDYIPGADANMR
                     VLTCYSDQNAKVRFAALGHVLLEDHTPTAIGNPVVIINERNDEIVRQATKFLEHVGYT
                     GFSNFDIKYDPRDNTYKFFEINVRLGRSNFYVTGSGFNTVKWIVDDLVYNKELPYTVA
                     DNEHLYTVVPKGVIKRYVPDEEQKERALRLFDENKVTYPLLYEADRSPARDFYVYAAL
                     YNQYRKFSKYSYI"
     gene            complement(3019..3870)
                     /locus_tag="PLD98_04530"
     CDS             complement(3019..3870)
                     /locus_tag="PLD98_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05547.1"
                     /translation="MKKIKIGALTLAVLLLFTSCGGGSFDSFKRHTDPATQNPSENPT
                     EITTVPDTSPVQIEMSKSEILRIYTQAANKVKTEHPGFKRTAEKTLLEVRADTGYNAI
                     IENIMRGIMNDILSDGSSVFESPMAIIPKGNSLGVKTYFPIFGESYGCGISGTDIISA
                     AQCVKVGNTYELTLYFNDINNPEPGSGEFGRIMSPVRKTAIQDAAASHAATPGRDILT
                     FETNYTNSYLKCAVDIETNRMLSLEQNMTINISVKAQISLFGITTNLYGGTGTIKYIL
                     IFSEFAW"
     gene            complement(3945..4640)
                     /locus_tag="PLD98_04535"
     CDS             complement(3945..4640)
                     /locus_tag="PLD98_04535"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQA05548.1"
                     /translation="MTQSKKDAAAAIIKSAVAMTLFFIAVFNYNDLRNVDVEGLVARA
                     GNIYWAFLVVLIIFFVKSVLFILPASLVYVAVGAALETVPAVTINLIGIVLEVTVTYL
                     LGLFLGGAYVEKLLSKRKKTKKLLEMELQNKPWLLMGIRFFPVFPIDFVSLFYGASKA
                     NFIKYLALSVLGLAPRVIAFTILGDALFEKIPMHIIILAVICLIPVFAAWTLFKSFVL
                     DKRKQEKEIKTKG"
     gene            4987..9420
                     /locus_tag="PLD98_04540"
     CDS             4987..9420
                     /locus_tag="PLD98_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05549.1"
                     /translation="MKRSTKVLSALLAAVLIITSAYVGIVAAAQDIDLEQKYDELADL
                     LASEYVKDLSNYSTKSETDEDDLVPYGGYKVYVTVTARDNLNGDIARAAEKFYEIANI
                     IKSTEYGVGYYNALMIINAVKEELAKRMGDKITVMEPYRAEIPVQKEVTVFVEDPNNP
                     GTYIETTEIRTVMETVTLYKPVEYERYRYYNVPEVLSYFIGEQTGISVVNWFYDYSFE
                     VKTDIDEALMAFDSVEDLPYRINVKYVKFTWKYARIAAENNTSVYYVLSDIIREAPEG
                     APYNYAETSIQIALQQAHMRLFGATSNLLTTDYTGYTADQLLAVRNSVKDYVVDLLRH
                     SDRLLVHFFGDKLSNLMNWYYSTEIPIQYPITREVKEGVTYSANLQRLNQAVDKIDAL
                     FLSPDVGTILKSFIDTTDDKYKGSPMYGVKYNNAKELAEVLLKDFIFRDNIVTSIFLM
                     LYPMVYEMLADMNSDVPILDLFNELRGEGIYLQFQYVRDHLPAKYQNIKNHMNAAISQ
                     GYVKDANSWAGFEEYVDSLPKGNKLNWGVDEAYDQKTAFINAVAAIFAPVEPLLWTLF
                     GDVKFYIEVAGRAAYASIQRMELYQKLIIPLFETLGIKNFITYDQFKPLRSHTDIMNA
                     ILGPIIDWVLNDVASKPITALTSAIPNLSMFLTSQNGKSPVQSIIDSVEVYLEAKVAG
                     ITVYTMEMPFRDLLSDYYPYLGGGLNGVLNLVSEMSGYDEEEEDEGFNIDYLPPILDA
                     AIQARGQIVRNKTFPSGSMTRDFVVVYDPSAGIDYRGQVLFALLRWVFTGINSRNYNY
                     STGKWADKTLLESFGLTIDDLDAELADMFGMPAGSRILSETIGGIAIHPNDVILAILE
                     LMFPKETGKFPTDGTYSPNPYVYPMQELTYSPEIVAQKSFGNRVQYNESWTKEKAEYL
                     AAATPIMVERLLKALKIEGINSVTDILEDMLVKEVFTQEMLTKIALMIYPMITELNID
                     SILSVALGLDYSPKTIAQRMAYLNNRAGRPASEVQLALENVAREDWHNNAAYSNVYNK
                     WNEALLFEHEFVPYIETIVEVNPETGEATTKTQYNHIVVPTGDRPLDWGFEDGDRAAW
                     LDNLVALLSPFADILQVILADRNLNLINLIDIPGYAGYQYSIIPLLEALGCDNIKTYA
                     QYKAALSNPITGPIDTFYLILNPLIGLFDRIMADPMRTILNILPNLMYFISIGGLNVV
                     INNLLHFVYVFADVVKPIVNAYPIVNALLDSLRLNGMSLGLTIPVDINVNYLMDRILF
                     RLVGDGIPIGDSNMKLNIKLPSADITMLVAGEPIYYEDRPGKQMSKTQRPGVILIQGN
                     NGADLLTVLLKYAFSVLFYSDNADEIGRFVSEYFSLNEEDTATLFEVLRFFNSKLNEM
                     GGEYMAMSMLYTLVSTLFPYTEDLVKLFENSQYGLMDVIGELPNVFEGDTSGLMLIIN
                     DIREGQPQDPNSTLTLFGRIIQILKDFFAKVQLFFSVVFNR"
     assembly_gap    9613..9712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9713..9908
                     /locus_tag="PLD98_04545"
     CDS             <9713..9908
                     /locus_tag="PLD98_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05550.1"
                     /translation="PPQCERKSHRSLAERNSRPPQKRLFRHAPKGAARPAAALSPPPR
                     HTGFPAPSIAAKKGFSPRLL"
     gene            complement(9983..11008)
                     /locus_tag="PLD98_04550"
     CDS             complement(9983..11008)
                     /locus_tag="PLD98_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQA05551.1"
                     /translation="MRKYLLPKEGRFYKANLHCHSTLSDGDYTPEQLKEHYKSRGYSI
                     LAITDHEWLNEHSYLDDEDFLTITGFELEVLEPTHLPNKQKKVCHINFFAKEQKNTTL
                     FAFNPKYFWSGNREKAKTLKHTGPDDYERVYSAEGVNDILRLAKENGFLSCLNHPDWS
                     LQNLDDYRDYDGFTMMEIYNYSSDIDGSYAYAAKDYDNMLRMGKKLFVCASDDNHDRF
                     AVNHPHHDSFGGFTYIKAPELKYEAVINALEKGDFYASMGPEIHELYAEDGKIYVKTS
                     PAKRIIMIPKGRNYNCVSAFEGEHVTEAVFELNDLEYEEYFRFEVIDERGRRAATRAY
                     YLNEILG"
     gene            11345..>11661
                     /locus_tag="PLD98_04555"
     CDS             11345..>11661
                     /locus_tag="PLD98_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05552.1"
                     /translation="MRTKPGNLKAAFNRTVVLVLTLCMVAGVMAAVITQTAPKAEAAE
                     LTANSAASLQTALNNAANGDTIKVTANIDMARTALSVSGSKSITLDLQSYTVSWSGDR
                     NNRL"
     assembly_gap    11662..11671
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11672..>12666
                     /locus_tag="PLD98_04560"
     CDS             <11672..>12666
                     /locus_tag="PLD98_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05553.1"
                     /translation="FTVGSGVTLTIRGSSSGTLSAYQDHTGQINCNASVEFIRTSGTV
                     ILESGNISAETYVSGNDTGSFDKGADGYSDPLGVAVKSGGRFVQNGGAVNVKSYLHLS
                     PRGTVNTSTDPVVYIRLKGIVIDSGGTGEMNAGTLDISSDAGVKNNGREDKRIEHYSY
                     AIVNEGSFTFNGGTITHWAQSRSTKKPRVTHYQYIFAGSKSPVITGGNATSTISRDGS
                     PGSTTWVHQAIETNSYIDAYGNTVSNVSGANGGSPTSHGGQTFISGGTPYSGSTVLRI
                     EYDYLGTITSSPNANIGTVTVSSNAGWKSAVTNPSTGSQIYNVANGANITINPIV"
BASE COUNT         3226 a         3138 c         2923 g         3269 t
ORIGIN      
        1 gagaaggttt tcaccttgcc cctctttgcc acctcggcgg tgacatagct ggaatcaatg
       61 ccgcccgaga ggaaacagcc cacctcaaca tcgctgatag tatggatatc aacggacttt
      121 gatagggtct cggaaataag ctcctcaaag tactcgattg acttttcgtc gtcgatgcga
      181 tactccatct cgaagtattt ttgtatcgtg agattgccgt ccttcagaat atagtaagat
      241 gacggcggca gtttaaatac attcttaaag aaggtctgct cggaagggat gtactcaaag
      301 caaaggtact cgggcagcct ttctttgtta aacacctttt taaagccggg gtggggcaga
      361 aaagccttaa tttcggaggc gaaaaagaaa gcgtctccgt ttttgtagta gaaatagggc
      421 ttaatgccga aaatgtccct tgctccgaaa agggtctttt ccttcttgtc ccaaatagcg
      481 aaggcaaaca taccgcgaag cttctcgcag accttctcgc cgtactcctc atagccgtga
      541 agaataacct cggagtcgga gttggtggca aagacatgcc ccttttctat cagctctttt
      601 cttatatcct taaagttata aatctcgccg ttatagacca gcacctttgt gccgtcctcg
      661 tttaaaatag gctgtttgcc gccctcaagg tcaataatgc taaggcgcct aaagcccatt
      721 gaaattttgt cgtctatata ataatcctca tcgtccgggc ctctgtgagc gattttgtcc
      781 gccatggagc ggactatgct catattaagc tcagcgtcat ctgtgtcgtt aagaaagccc
      841 acaaaaccgc acatattatt ctccttaaac caaaatcaca tttccacagc caattccttg
      901 ccgcaaagct caatgctccg ctttgccaaa acctcaagtg aatctataac atcgggccgc
      961 tttatattaa actcgtgctt tattaccgaa agctcggtgc agcccagaac cacagcgtcg
     1021 cagccgtcgt ttttcatggt ttcgataatg tttaaaaaga gctgtaaatc aactcttttg
     1081 cccgccttta cctgattgta gattatatgc ataagggact gctcgtcctg tatggacggg
     1141 acggcgggct caatgtccct tttttcgcaa gccctttgat aagattttgc cgtaatggtg
     1201 cctttggtgg cgagaattcc gagttttctg agattcttca ccgtcttttg ggcgtagtct
     1261 acggtttcct caataatatt gactaaaggg atacttacat tgctctgtat ctgctcataa
     1321 aaataatggg cggtattgca ggggatgact ataaagtcaa cgcccatctt ttcaagcttt
     1381 ttcgcgtcct caatcataac gggcaggggg ttgggctttg agttgtcaag tatatactcc
     1441 gtcctgtccg gtatggaggc gtgatttaat attactgaag aaatatggct ctggtcgcag
     1501 tccgcctttg tcatctccac aaggagcttc gcgaaataaa cggtggcaag ggggccgacg
     1561 ccgcccaata cgcccaaagt tttttctttc atatttgtct cctttgccgc gaaccaaatt
     1621 tataagggct tagatatagg agtacttgct gaatttcctg tactgattgt agagagcggc
     1681 atagacatag aaatctcttg cggggctcct gtccgcttcg tacaaaaggg gataggtgac
     1741 cttgttttcg tcaaagagcc tcagcgccct ttccttctgc tcctcatcag gaacatacct
     1801 cttgattacg cccttcggga caacggtgta aaggtgctca ttgtcggcaa cggtgtaggg
     1861 cagctctttg ttatacacaa ggtcgtccac aatccacttg acggtattga agccgctgcc
     1921 ggttacatag aagttgctcc tgcctaggcg cacattaatc tcaaagaatt tataggtatt
     1981 gtctctgggg tcgtacttta tgtcaaagtt ggaaaagccc gtatagccca catgctcaag
     2041 gaatttggtc gcctgacgga caatctcgtc gttcctctcg ttgataatca caacggggtt
     2101 gccgatagcc gtgggggtat ggtcctcaag cagaacatga cccagcgccg caaagcgaac
     2161 ctttgcgttt tggtcggagt agcaggtcag aactctcatg ttggcgtcgg cgccggggat
     2221 atagtcttga atcaggaatt tgtagttata tcccgcatcc tcagcctttt taagcatttc
     2281 cctaaggctc ttttcgtcct ggaagcggaa aaccttcttc ttgccttcga atttagcgta
     2341 gtgataaaga gccgaattgg cgggctttgc tatgacgggg taatcaaaat caaatttcag
     2401 gttgttctcc tcaccgcagt tgtagacata ggtcttgggg tgggggatgt ccaattcgtc
     2461 gcagatagcg tagaacttgt cctttaaaac aatgcggttc aaaagctcct catcgatgta
     2521 aggaatgata tagtagggct ttaaaacctc cttgttttcg ataaggattc tgacatacca
     2581 gtcgccgcag ccgaggacaa tcagcttttt cctgcccttg aactcctcgc ccactttgag
     2641 gagagtgtca ataaggacct cttctttctc aagaccgggt ataaccctgt tctctattat
     2701 cttgctgcgg gctatgaatt cggatttgag catgctgagc acaagggact tgatgccgta
     2761 ctcctcgtga aagcttctcg cgtggctgta tgctgttata tcggcgccca gaataaccgg
     2821 gagaaattcg gtttcgttaa acttcatatc aaagcctctt tcaacttcaa attgccttcg
     2881 gcaataaaat tatttacata aatttaccgt gtgatacaaa aaaaccatac tttgtaaatt
     2941 atattgccat acattctcaa agtcaacaaa agggaatcaa aaatgcgata aaaaagcggg
     3001 ctttaagggc ccgctcgttt accatgcaaa ctcgctgaaa atcaggatat attttatcgt
     3061 tccagtgccg ccgtaaaggt tcgtggtgat tccgaaaagg ctaatctgcg ccttgaccga
     3121 aatgtttatg gtcatgttct gctcaaggct cagcatcctg ttggtttcga tgtccacagc
     3181 gcactttaga tagctgttag tgtagttggt ttcaaaggtg agtatgtccc tgccaggggt
     3241 ggcggcgtgg gatgccgcag cgtcctgaat agcggtcttt cttactgggg acattatcct
     3301 gccgaactcg cccgaaccgg gctcgggatt gtttatgtcg ttaaagtaaa gggtcagctc
     3361 gtatgtattt ccgactttca cgcactgggc ggcgctgata atatccgtgc ccgaaattcc
     3421 gcagccgtag ctctcgccga aaatggggaa ataggttttg acgccaaggc tgttgccctt
     3481 tggaataata gccatgggac tttcaaacac gctgctgccg tcggagagaa tatcgttcat
     3541 aatgccccgc attatgttct ctataatggc gttgtatccc gtgtccgccc tgacctcaag
     3601 gagagttttc tcggcggtcc gtttaaagcc ggggtgctcg gtctttactt tgtttgccgc
     3661 ctgggtgtag attctcaaaa tctcgctttt ggacatttct atctgcactg gcgaggtgtc
     3721 gggtacggtg gttatttcag tcggattctc cgacgggttt tgcgtggccg ggtcggtgtg
     3781 cctcttgaat gagtcaaagc tcccgccgcc gcaggaggtg aaaagcagca aaaccgcaag
     3841 ggtcagcgcg ccgattttaa tttttttcat tatcatcacc acaaagcatt tatttgccct
     3901 ataaaaatta attttagccc gtaccgagtg agatttttgt tgaattaacc ctttgttttg
     3961 atttctttct cctgttttct tttgtccaat acaaagctct tgaaaagcgt ccaagccgca
     4021 aagacgggga taaggcagat tacagccaga atgattatat gcatcgggat tttttcaaac
     4081 agcgcgtcgc ccagaatagt aaaggcgatg actctgggag cgagccccag aacagacagc
     4141 gcgagatact ttataaaatt cgcctttgag gctccgtaga aaaggcttac aaaatcaatg
     4201 gggaagacgg ggaaaaacct gattcccatg agcagccacg gcttgttctg aagctccatc
     4261 tcaaggagtt tcttagtctt tttgcgtttg ctcaggagct tttcaacata ggctccgccc
     4321 agaaacagtc ccaaaagata ggtgaccgta acctccagaa caatgcctat taggtttatt
     4381 gtgaccgcgg gaacggtttc aagtgccgcg ccaaccgcca catagacaag cgacgcgggg
     4441 agaatgaaca aaacagattt tacaaagaag atgatgagaa ctacgaggaa tgcccagtat
     4501 atattgcccg cccttgccac caagccttca acatccacat ttctaaggtc gttgtagtta
     4561 aaaacggcta taaaaaacag cgtcatggca acggcggatt ttattatcgc cgctgcggcg
     4621 tcttttttgc tttgcgtcac cgattcactt cctatttttc agctttataa cattataaca
     4681 taagcaaggt ctatattaaa gttttttata tttttcatct aaggctgtga ttattgcaaa
     4741 aaaggaaaaa gaaagtgtca caaatgggca gattgttaaa atttcacagt cctatttggg
     4801 caaaatgacc aaaccccaaa tgccgtaaga tatgttaatg gacaaattgc cccgtattgt
     4861 cgcttgaaat attataacta tcctatataa ttaaattgaa taaaagtcta aagatataga
     4921 atttttgaat tctatctgtt tctgcgcaga gtttgactta gggtacctaa aaggagtgaa
     4981 tgttaaatga agcgttcaac aaaggttttg agcgcccttt tggcagctgt gctgattata
     5041 acatctgcct atgtgggaat agtagcggcg gctcaggata ttgaccttga gcaaaagtac
     5101 gacgagcttg ccgatctgct cgcaagcgag tatgtaaagg atttgtccaa ctattccaca
     5161 aagagcgaaa cggatgaaga cgaccttgtc ccctatggcg gatataaggt ctatgtcacc
     5221 gttaccgcga gggacaacct taacggcgac atcgcaaggg cggctgaaaa attctacgag
     5281 atcgcgaaca tcataaagag caccgaatac ggtgtgggct attacaacgc ccttatgatt
     5341 ataaacgcgg tcaaagaaga gcttgccaag agaatgggcg acaaaattac ggttatggag
     5401 ccttacaggg ctgaaatccc cgtccagaag gaagtaaccg tatttgtgga ggaccccaat
     5461 aatcccggta catatataga gaccaccgaa atcagaacgg ttatggagac cgtcaccctc
     5521 tacaagccgg tggaatatga aaggtaccgg tactacaatg tacccgaagt actctcctat
     5581 tttataggcg aacagacagg catcagcgtc gtcaactggt tctatgacta cagctttgaa
     5641 gtaaagaccg acattgacga ggctctgatg gcttttgatt ctgttgagga cctgccctac
     5701 agaatcaatg tgaagtatgt aaagttcacc tggaagtacg caagaatcgc cgctgaaaac
     5761 aacaccagcg tttactatgt tctctccgat attataagag aagctcccga aggcgccccc
     5821 tacaactatg cggaaacaag tattcagata gctctgcagc aggctcacat gagactgttc
     5881 ggcgccacat caaatcttct cacaaccgac tatacaggct ataccgcaga ccagcttctc
     5941 gcggttcgca acagcgtaaa ggactatgtt gttgaccttc tccgtcactc cgacaggctg
     6001 cttgttcact tcttcgggga caagctgtcg aacctcatga actggtacta ttcaaccgaa
     6061 attccgattc agtaccccat cacaagagag gttaaagagg gcgttacata ttcagctaat
     6121 ctccagcggc tcaaccaggc cgttgacaag attgacgctc tgtttttgtc tcccgatgtg
     6181 ggaacaatcc ttaaatcctt tatagacacc acagacgaca agtacaaggg cagccccatg
     6241 tacggcgtaa agtacaacaa cgccaaggag cttgcggaag ttctgcttaa agacttcatc
     6301 ttccgcgaca acatcgtaac ctcaattttc ctgatgcttt acccgatggt ttacgagatg
     6361 cttgcggaca tgaactcgga tgtgccgatt ctcgacctct tcaacgagct cagaggcgaa
     6421 ggcatttacc tgcagttcca gtatgtaagg gaccatctgc ccgcaaagta tcagaacata
     6481 aagaatcaca tgaacgcagc catttcccag ggctatgtaa aggatgccaa cagctgggca
     6541 ggctttgagg agtatgtgga ctctctgccc aagggcaaca agctcaactg gggtgttgac
     6601 gaggcatacg accagaaaac cgccttcatc aacgcggtag ccgccatctt tgctcccgtt
     6661 gaaccccttt tgtggaccct tttcggcgat gtaaagttct acattgaagt agcaggcaga
     6721 gccgcatatg cgtccattca gagaatggag ctttatcaaa agctcattat cccgcttttc
     6781 gagaccctgg gcattaagaa cttcattaca tacgaccagt tcaaacccct gcgttcgcat
     6841 accgacataa tgaacgcgat actgggcccg attattgact gggttctcaa tgatgtggca
     6901 tcaaagccca tcaccgcttt gacttcggca attcccaacc tttcgatgtt ccttacctct
     6961 cagaacggca agagccccgt tcagagcatt attgactccg tagaggttta tcttgaggca
     7021 aaggttgcgg gcattacggt ttataccatg gaaatgccct tccgtgacct gctttcagac
     7081 tactatccct atctgggcgg aggcttgaac ggcgttctga atctcgtgtc cgagatgtca
     7141 ggctacgatg aggaggaaga agacgaaggc ttcaatattg actacctgcc tcccattctt
     7201 gacgccgcta ttcaggcaag aggccagata gtaagaaaca agaccttccc ctccggctcc
     7261 atgacaagag actttgttgt ggtctatgac ccgagcgccg gcatcgacta cagaggtcag
     7321 gttctctttg cccttctcag atgggtattc acaggcatta acagccgcaa ctacaattac
     7381 agcacaggca agtgggcaga caagactctg cttgagagct tcggtctcac catagatgac
     7441 cttgacgcgg agcttgccga tatgttcgga atgcccgcgg gcagcaggat tctttccgaa
     7501 acaatcggcg gaatcgcaat ccatcccaac gatgtaatcc ttgccatcct ggagcttatg
     7561 ttcccgaaag agacaggcaa gttcccgaca gacggcacat attctccgaa tccttatgtc
     7621 tatcccatgc aggagcttac ctattcgccc gaaatagttg ctcagaaatc cttcggcaac
     7681 agggtacagt acaacgaaag ctggacaaag gaaaaggctg aataccttgc cgccgcgact
     7741 cccataatgg ttgagagact tctgaaggct ctcaagattg agggcataaa cagcgtcacg
     7801 gatattcttg aggatatgct cgtaaaagag gtatttactc aggagatgct cacaaagatt
     7861 gccctgatga tttacccgat gattacggag cttaacatag actccatact ctccgttgcg
     7921 ttgggtcttg actattcgcc caagacaata gcccagagaa tggcttacct caataacaga
     7981 gctggcagac ccgcctcaga ggttcagctc gcccttgaga atgtggcaag agaagattgg
     8041 cataacaacg ccgcatactc caatgtctac aacaagtgga acgaggctct gcttttcgag
     8101 cacgagttcg tgccctatat cgaaaccata gttgaggtca accccgaaac aggcgaggca
     8161 acaaccaaga ctcagtacaa ccacatagtc gttcccaccg gcgacagacc ccttgactgg
     8221 ggctttgagg acggcgacag agccgcatgg cttgacaatc tggtagctct cctttcaccc
     8281 tttgccgaca ttctgcaggt aatccttgca gacagaaatc tcaacctcat aaacctcatt
     8341 gacatccccg gctatgcggg ctatcagtac tccattatcc cgctccttga ggctctgggc
     8401 tgcgacaata tcaagaccta cgcccagtac aaggcagcgc tcagcaaccc gataacaggt
     8461 cctatcgata ccttctacct tatcctcaac ccgcttatcg gtctctttga caggataatg
     8521 gccgacccga tgcgcacaat cctcaacata ctgcctaacc ttatgtactt catctccata
     8581 ggcggactca atgttgtgat aaacaacctg ctgcactttg tctatgtgtt tgcggatgtt
     8641 gtcaagccca tcgttaacgc ctatccgata gtcaacgccc tgcttgactc tctgcgcctt
     8701 aacggaatgt ccctcggact taccatcccc gtcgacatca atgtcaacta ccttatggac
     8761 agaattctgt tccgcttggt aggcgacgga ataccgattg gcgacagcaa tatgaagctt
     8821 aacatcaagc tgcccagcgc cgacatcaca atgcttgtcg ccggcgagcc catctactac
     8881 gaagacagac ccggcaagca gatgtcaaag actcagcgcc ccggagtaat cctcattcag
     8941 ggcaacaacg gcgctgacct gctgacggtt ctcctgaaat acgccttcag cgtcctgttc
     9001 tactcggaca acgctgatga aataggcaga ttcgtgagcg agtacttcag cctcaatgag
     9061 gaagacactg caacgctctt tgaggtactg cggttcttta acagtaagct caatgagatg
     9121 ggcggcgagt acatggcaat gtccatgctc tacacgctgg tatccactct cttcccgtac
     9181 actgaggatt tggtaaagct gtttgagaac tcccagtacg gacttatgga tgtcatcgga
     9241 gaactgccga atgtctttga gggcgacacc tccggcctta tgctgataat caacgacata
     9301 agagagggac agccccaaga cccgaactcc acgctgaccc ttttcggaag gattattcag
     9361 attctcaagg acttctttgc caaggttcag cttttcttca gcgtggtctt caataggtaa
     9421 cacaaatccc ccgattttcg ggggattttt aattccgaac aacagtcccc cgtaatgggg
     9481 gatttttgtt ttcccgctgt cttttctgcg gtttgggtgc atagacagcc gcccctgcgc
     9541 gccgcgcgct gttgggggag ccgcacagta ctgcttgccg taccgattcc ccgccccgcc
     9601 gcagtgtgag cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccgccgc
     9721 agtgtgagcg aaaatcccat cgcagccttg ccgaaaggaa ttcccgcccg ccgcaaaaaa
     9781 ggcttttccg ccatgcgccg aaaggagcag cccgccccgc cgctgcgctt tccccgccgc
     9841 caaggcacac gggtttcccc gctccatcca tagccgccaa aaagggcttt tcgccccgcc
     9901 ttctctgacc cgcattaaag cccaacagct ttaggactgc atacggtcaa aaaaaccccg
     9961 ctcattgagc ggggttttta tattagccta aaatctcgtt aaggtaatat gcccttgtgg
    10021 cggctcttct gccgcgctcg tcaattacct caaagcggaa atactcctcg tactcaaggt
    10081 cgttcagctc aaataccgcc tcggtaacat gctctccctc aaaagcggag acgcagttgt
    10141 aattcctgcc tttgggaatc atgataatgc gctttgcggg ggaggttttg acatatatct
    10201 ttccgtcctc ggcataaagc tcatgaattt cgggacccat ggaggcgtag aaatcaccct
    10261 tctcaagggc gtttatgacc gcctcgtatt taagctcggg tgctttgata tatgtaaagc
    10321 cgccgaatga gtcgtgatga gggtgattta ccgcaaagcg gtcgtggttg tcgtcgctgg
    10381 cgcagacaaa gagttttttg cccattctca gcatattgtc atagtctttc gccgcatagg
    10441 cataggagcc gtcaatgtcg ctggagtaat tgtatatctc catcatcgta aagccgtcat
    10501 aatccctgta gtcgtcaaga ttctgaaggg accagtcggg atggttaagg caggagagga
    10561 agccgttttc cttcgcgagc cttaaaatat cgttgactcc ctcagcggag taaacccttt
    10621 cgtagtcgtc gggacccgta tgctttaatg tctttgcctt ttcacggttg ccgctccaga
    10681 agtacttggg gttaaaggca aatagggtgg tgtttttctg ctctttggca aagaagttta
    10741 tgtggcagac cttcttctgc ttgttgggca ggtgagtggg ctccagaacc tccagctcaa
    10801 agccggtaat ggtgagaaaa tcctcgtcgt caagatatga atgctcattg agccactcgt
    10861 ggtcggttat ggcgaggatg ctgtagcctc tcgacttgta gtgctcttta agctgctcgg
    10921 gggtgtagtc gccgtcggac agggtgctgt gacaatggag atttgccttg tagaacctgc
    10981 cctccttggg cagtagatat tttctcatac atttcctctt ttctaaatgt aatgcggttg
    11041 gcaatcaagg gtattttatt cccataaatt cggggtgtca acctccccgg taaaaatctc
    11101 gattgagtac tttcctcccg agcaaagagg tcgacaaaat acgccggact ttccgtaaat
    11161 cggctatgtt caaagtgacg aaaatttaca ttaaggcttc aaatagcttg acaggcagta
    11221 gggcggaata tataatcatt atgtataatt gtctagctgt gtccctatgt gtttttatac
    11281 agctttacag aatctgtttg ataattgcct ttcttatccc gctaacttta aggaggaaca
    11341 aattatgagg acaaagcctg gaaaccttaa agctgccttc aacagaacgg tggttttggt
    11401 tttgacttta tgtatggttg caggcgtaat ggcagcggta attacccaga ccgcacccaa
    11461 ggcagaagca gctgaattga cggcaaacag cgccgcttca ctacagactg ctttgaacaa
    11521 tgctgccaac ggtgatacaa tcaaggtcac cgccaatatt gatatggcaa ggacagccct
    11581 ctcggtttca gggtcaaagt ccataaccct tgatttgcaa agctatacag tttcttggag
    11641 cggtgacaga aataacaggc tnnnnnnnnn nttttaccgt gggttccgga gtaaccctta
    11701 caatcagggg cagctcttcc ggtactctca gtgcttatca ggaccatacg ggtcagatta
    11761 actgcaatgc ttcggttgag tttatccgta caagcggaac cgttattctc gaaagcggca
    11821 acatcagtgc cgagacctat gtcagcggta atgataccgg cagcttcgac aaaggcgccg
    11881 acggttattc cgaccccctc ggtgtcgccg taaaatccgg cggccgcttt gttcagaacg
    11941 gcggcgcagt caatgttaag tcttaccttc acctttctcc cagaggtacc gttaacacca
    12001 gtacagaccc cgtcgtttac atccgcttaa agggtattgt cattgattcc ggcggaaccg
    12061 gcgaaatgaa cgccggtaca cttgatatca gctcagatgc cggtgtaaaa aacaacggtc
    12121 gtgaagataa gcgtatagag cattattcat acgccatagt aaacgagggt tccttcacat
    12181 tcaacggcgg tactattact cattgggccc agtcccgttc taccaagaag cccagagtaa
    12241 ctcactatca gtacatattc gcaggttcca aatccccggt tatcacaggc ggtaatgcaa
    12301 cctcgactat ttcccgtgac ggctcccccg gctccacaac atgggtacac caggctattg
    12361 agactaacag ttacatagac gcatacggca atactgtatc gaatgtctca ggcgctaacg
    12421 gcggctcgcc caccagccac ggcggtcaga cattcatttc cggcggtact ccttacagcg
    12481 gcagcacagt tcttcgtatc gaatatgact atctcggcac aatcacctca tcgccgaatg
    12541 caaacatagg taccgttact gtttcaagca atgcaggctg gaaatctgct gttacaaatc
    12601 caagcacagg cagccagatt tacaatgttg caaacggcgc caatataaca attaacccga
    12661 tagtaa
//
LOCUS       DAONVQ010000050        12638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3823_length_12638_cov_4.512994, whole genome shotgun sequence.
ACCESSION   DAONVQ010000050 DAONVQ010000000
VERSION     DAONVQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12638
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3823_length_12638_cov_4.512994"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLD98_04565"
     CDS             <1..347
                     /locus_tag="PLD98_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05554.1"
                     /translation="DLQSSDSSGVDFMTNPNAITIIQRGIYRVDAMVMGKPNVGAAIS
                     LGVRNSGFSMSNMYQEETLSANESVTFSLSNFVELNAGEYLTLAMTSVPDSTFNLPSF
                     GIGARLSVQQIM"
     gene            complement(387..1220)
                     /locus_tag="PLD98_04570"
     CDS             complement(387..1220)
                     /locus_tag="PLD98_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05555.1"
                     /translation="MTIEEEGLYLVDWYVVTQSAPGVTSVSFNLVTGDGQVFESNMPT
                     KTGIMSGLAIINVAVLPFTIQLVNQSNTTVYFSNAVGAKANLRIVRLDHLIPDNSRCF
                     AMDQLSFVMKQLADAYSGENIRIFTNIFGVIDNTLYGYYQAPDTSSSPLLLISDPLNA
                     LNLNHLVALYFFNVPYDESITFLQPPDPFPQNCDTDLIKNIHDFLSVGDNISFLAGPN
                     ISGSGDIIKNEYGLLVLGDDTSSLYLPTPNLTVISIESNGEDFAGRSSKGHRPLIIET
                     K"
     gene            complement(1476..2486)
                     /locus_tag="PLD98_04575"
     CDS             complement(1476..2486)
                     /locus_tag="PLD98_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HQA05556.1"
                     /translation="MYRTITDETKTFKEGVKPRLFKPDFEREPIENSFQLEESLKRQV
                     EKAVSDGKAALALSGGIDSAILARFMPKSSTAYTFKCVVPGVQVIDETLAAARIAEEC
                     GLKHKIVEIYWEDFEQYAPLLMAHKGAPIHSIEVQIYKAGLKAKEDGFERLIFGESAD
                     CLYGGHDKLLSRDWTVGEFIDRYSYVRPYMALKDFVMVTEPVAKHAKDGFVDVHGFNS
                     DVYFKESVGSYINALQATGVEFFSPYVNTYLNAKFDIERVRRGDGKYLVREVFKRLYE
                     NFEIPEKIPMPRPTNEWLKNWSGPKRDEFWPNCTVNMTGDQKWLVLCLEWFLNLLDDG
                     KI"
     gene            complement(2535..2951)
                     /locus_tag="PLD98_04580"
     CDS             complement(2535..2951)
                     /locus_tag="PLD98_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HQA05557.1"
                     /translation="MTIGYTAGVFDLFHIGHLNLLKNAKGMCDKLIVGVTADELVTYK
                     GKRAVIPFHDRIEIVRSIKYVDAAVAQYDMDKLTMCKKLGASILFVGDDWYGTEKWEN
                     YEKEFSEAGIKIIYFPYTQGVSSTKITQALNLLRSE"
     gene            complement(2948..3769)
                     /locus_tag="PLD98_04585"
     CDS             complement(2948..3769)
                     /locus_tag="PLD98_04585"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HQA05558.1"
                     /translation="MSDKKLFTLSELQDVLYDMLKAFAEFCDENDLRYYLYGGTLLGA
                     VRHQGFIPWDDDVDVAMPRPDYERFIELTKNSPWEYYAVCQYTQPFIKMVDTRTVMEE
                     RQLRDELGMQSAFIDIFPIDGLPESGEERQKHFRKLAILKQCLYFSIFDIKKIKSSNF
                     LKRIAHTMIYIAFSKIDYKKIRDAIAEEAKRYPYETSEYVSVGIWGKAERDLNKKEDL
                     EKRIRLKFRDSYLWCQGNYDESLRLKYGDYMQLPPEDKRQPFHGIYYWKKENDKG"
     gene            complement(3771..5066)
                     /locus_tag="PLD98_04590"
     CDS             complement(3771..5066)
                     /locus_tag="PLD98_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HQA05559.1"
                     /translation="MQYKNKVVKNSAWIIACKVAQSVIGLVVSMFTARYLGPSNYGLI
                     NYASSLVAFFVPLMYLGLNSILVQEIVIKPQKEGETLGTALTMSLVSSLFCILGVFAF
                     TTIVNAGEKDTIIVCVLYSILLIFQAVEALQYWFQAKLMSKYTSLTMFAAYIITALYK
                     IFLLASKKSIYWFAVSNALDFAIIAVVLLFIYKRLGTQKLSFSPDTAKLLFSKSRYYI
                     VSSMMVVVFGQIDRVMLKLMIDEKAVGLYSAAVASAGVTYFVFAAIIDSFRPVIFESK
                     KADHASFEKNVSRLYSIIIYFALVQSAGIALFAELVVKILYGEQYLASIPALRIIVWY
                     LAFSHMGSVRNIWILAEGKYKVLWIINLCGALTNVILNLIFIPLWGINGAALTALITQ
                     IFTNFILGYIIKPIRRNNTLLLKGLNPNILIELVRKKDV"
     gene            5555..5881
                     /locus_tag="PLD98_04595"
     CDS             5555..5881
                     /locus_tag="PLD98_04595"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HQA05560.1"
                     /translation="MTFYESLMVGLFCFTGVFLVLVVLNLLIRLFSAVLGSAAKKTSS
                     AAKPEPPAATSQQTVKTPETKDSSNQVELIDVDDATAAVIMAIVSHETGIPLSNLEFK
                     YIKLVQ"
     gene            5943..7076
                     /locus_tag="PLD98_04600"
     CDS             5943..7076
                     /locus_tag="PLD98_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HQA05561.1"
                     /translation="MYIEAIRKIWEMSGFAALTWQHGVMILIACVLMYFAIVKKFEPL
                     LLLPISFGMLLANLPLAGLMGAPMGEEAGGLLYYLYQGVKLGIYPSLIFMGIGAMTDF
                     GPLIANPSSLLLGAGAQFGIIVAFIIAVALGFDPKAAASIGIIGGADGPTAIYLTSKL
                     SPDLLPAIAIAAYSYMALIPMIQPPIMRLLTTEKERKIEMKQLREVSKLEKVCFPIIV
                     TILCVLLLPSVAPLIGMLMLGNLFRESGVVERLSDTAQNALINIVTIFLGVTVGATSS
                     AERFLRLETIKIIALGLAAFAFSTVGGILLGKLMCVLTKGKVNPLIGSAGVSAVPMAA
                     RVSQIEGQKANPRNFLLMHAMGPNVAGVIGSAVAAGILLALFG"
     gene            complement(7119..8390)
                     /locus_tag="PLD98_04605"
     CDS             complement(7119..8390)
                     /locus_tag="PLD98_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQA05562.1"
                     /translation="MVQALVLFLLTYVLLMALPKYRPYIALASAALFVVLGILPASKV
                     FASVDWNVIMMIAGTMGVVSLFIESKMPALLADLIIMKMPNVKWAIISLSLFAGLISA
                     FVDNVATVLMVAPVALNIAKKLNISPVKSIISIAIASNLQGAATLVGDTTSILLGSHM
                     NMDFLDFIVYQGKPGLFWIVQIGALAATLVLFYVLRKDNYSIDVKDKTRVEDYFPSVL
                     LVGTVVFLILASFIPADKKPAITNGLICIGFMIAGLIRETIRSRNLEAFKRTMKEIDY
                     FTLLLLAGLFIVISGITEAGVVTKISEIFVNLSGSNVFLMYTLLVWASVLFSAFIDNI
                     PYIATMLPVTAGIANLMGINATVLYFGLIVGATLGGNLTPIGASANIAGLGILKKNGY
                     EVKSAEFMKISVPFSLAAVMTGYILVWIFYR"
     gene            complement(8522..9430)
                     /locus_tag="PLD98_04610"
     CDS             complement(8522..9430)
                     /locus_tag="PLD98_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQA05563.1"
                     /translation="MKVLAFGEVLWDKFGNTREIGGAPFNFCAHLAKLGINSYSLTAI
                     GDDDLGEETLKIIDSLGVKTDFVRTVPYPTGTCIVTTDENGRPGYDLTQPAAWDYIEP
                     YTELVNRSAAGEFNLFYFGTLALRSDTSFETLKCLVNTGSFEHIFCDLNLRQSFYSDE
                     IIDFALKTASILKINREELRHLINNGFADADNNDTERAQKYACRSLSEKYAIKLILLT
                     LDKDGALIYDAGKDKFHSPSKPKSKAVSGVGAGDSFSACFIANFLSGVPTEECAERAI
                     LLSDYVVTKYGAVPDYPPELIRLITP"
     gene            complement(9574..12453)
                     /locus_tag="PLD98_04615"
     CDS             complement(9574..12453)
                     /locus_tag="PLD98_04615"
                     /inference="COORDINATES: protein motif:HMM:NF037374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HQA05564.1"
                     /translation="MLQKNADYTLSKTSDNYDRIQLNASFLNTLPLGEYTLTLSYNHR
                     RYILAEPYEGSDKLTDVTINLSVEKGAPTIELQVSPQNQTRPGSVTLSAVLPADATGT
                     LTFKAGTDVIATVPVGQSVQFTPAGSYDSYAFTVEYSGDSSYNSAVSAAVPYTFRKGI
                     QAELSFDENFPAVKTYGDADFTLGAIGGSGSGNISFTVLEGPARIINGNKVRITGAGT
                     VTIRATKAGDNDYNPITVDKSFTVNKKTVTISGISVSDKVYDGTDAAEIIGEPVLNGV
                     VSGDSVGVMRGTAFFQNKNAGTWTVYFTDFALTGTDAGNYLLASQPAPVTARITKAPL
                     NISVVIKTKQYNGLNDAQIESVNLSGLISGDNVAVAEPYPIARFTSVNAGKNIPVVFE
                     ESFSLIGTHADNYELIQPTNVTGSIENSFAAVKGIHYTTTTNEWANSDFVITAMPGYK
                     LSRVNNANAIWYDYLVNNDQGHYTTQFYVKDEASGLISQIAYESCKIDKEAPTAQISV
                     FYIFKDIPIDKNFGEISFGSHYFDASLYIIIQSIDSLSGVKSVEYYLSSDILTLNEVK
                     DVTDWNVYDDMFLINPDRRAVVYVKVTDNAGNADYFASNGIIIDATAPTVTGIEDGAV
                     YCGKAEFSVYDENFGAITDNSYEITAADGRYVITEPGEHLIIVYDNAGNTKVLTITVN
                     AEHTYDSDCDADCGICGEERVAPHSFSAEWRHDETHHWHECAGCGAKTDIAAHIPEED
                     DGDCTTPDLCSVCGAVLSAATEHDFTGECHHDAEGHWHECANDGCTVLCEKQPHEWDE
                     GTVILQPTARSKGTMLYTCLVCGETRTVDIPMLPPVITKGKGSVWNPKSAGTIVFKSS
                     AAFGDFVGVRLNGESLDESNYEAAESEDGIVVTLKEEYLKTLPTGTYTIGIESVGGIA
                     TAEFTVTEPGKTDSLLESIMRLFEYLAMILAFVSYFIKNYYDLFI"
BASE COUNT         3302 a         2810 c         2931 g         3595 t
ORIGIN      
        1 tggatttaca gtcaagcgat tcttccggcg tggatttcat gacaaacccc aacgctatta
       61 caataatcca gcggggtatt taccgcgttg acgcaatggt catgggcaaa ccaaatgttg
      121 gcgccgccat atctctggga gtcagaaaca gcggtttctc catgtcaaat atgtaccagg
      181 aagaaaccct gtccgcaaat gaatcggtaa ccttctcgct gtcgaatttt gtggagctga
      241 acgcaggaga atatctgact ctggcaatga cttcagttcc cgacagtacc ttcaacttac
      301 catcctttgg tattggagcg agactttcgg ttcagcagat tatgtaagag aatagagcct
      361 gcggtttatg gacgcaggct ctatttttat tttgtttcga ttatcaaagg tctgtggccc
      421 ttgctgcttc ttcccgcaaa atcttctccg tttgactcaa tgcttattac tgtcaaattc
      481 ggggtcggca aatagagcga tgaggtatca tcgcccaaga caagcaatcc gtattcgttt
      541 ttaataatat ctcccgaacc gcttatattc ggacctgcca ggaacgaaat attgtctccg
      601 acggaaagaa agtcgtgaat gtttttgata aggtccgtat cgcagttctg ggggaagggg
      661 tccgggggct gaaggaaggt gatggattca tcatagggaa cattaaaaaa gtataaagca
      721 acaagatggt ttaagtttaa cgcattgagc gggtcactga taagcaggag cggcgatgat
      781 gaggtgtcgg gagcctgata atacccgtaa agggtgttgt cgatgactcc gaaaatgttg
      841 gtaaagattc tgatattctc gcccgaataa gcatcagcca actgcttcat gacgaaagaa
      901 agctggtcca tagcgaagca gcggctgttg tcgggaataa gatggtcaag tctgactata
      961 cgcagatttg cctttgctcc aacggcgttt gagaagtaaa cggtagtatt ggattgattg
     1021 acaagctgaa ttgtaaacgg aagaactgcg acattgatta tcgcaagacc gctcattatt
     1081 cccgttttgg tgggcatgtt ggattcaaaa acctggccat cgcctgtaac cagattgaaa
     1141 gatacgcttg taacaccggg agcggactgg gtaaccacat accagtcaac cagatacagc
     1201 ccttcctcct ctattgtcac tacgccgctt aagtcgctat atgaaatatt tccttctgtg
     1261 actactgttt catcaaagac aatgttattg ttgttatcta cgggatctat attattcttt
     1321 tgaacctaaa gtaagatgtt tgccatattt ataacctctt tcatttttgc taatacataa
     1381 atatgccgga aagaggttat gtgttattta ctgttctggg ccattaaaca aggaaaaaaa
     1441 ataaggcggg gttaccgcct gaatttgttg gttgattata tttttccatc atctaaaagg
     1501 tttaggaacc attcgaggca aaggacaagc catttttggt cgcctgtcat gtttaccgtg
     1561 cagtttggcc agaattcgtc ccgtttggga ccgctccagt tttttagcca ttcgttagtg
     1621 ggtctgggca tggggatttt ttcgggtatt tcaaaattct cataaagcct tttgaaaact
     1681 tctcttacaa ggtacttgcc gtctcccctg cggactcttt caatatcgaa ctttgcgttt
     1741 aaataggtat ttacataggg gctgaagaat tcgacgcccg tagcctgaag ggcgttaata
     1801 tatgaaccga cgctctcctt aaagtaaaca tcgctgttga atccatggac atccacaaaa
     1861 ccgtccttgg catgttttgc gacgggctcc gttaccataa cgaaatcctt taatgccatg
     1921 tagggtctta cataggagta gcggtctatg aactccccga ccgtccaatc ccttgaaagc
     1981 aatttgtcgt gaccgccgta cagacagtcc gcgctttcac cgaaaatcaa cctttcaaag
     2041 ccgtcctctt tagctttgag tcccgctttg taaatctgaa cctctatgga gtgaatcggc
     2101 gcgcctttat gggccatgag aagcggcgca tactgctcga aatcctccca ataaatctcc
     2161 acaattttgt gcttcaagcc gcactcctct gcaattcttg cggctgcgag agtttcgtca
     2221 atgacttgaa ctccgggcac aacgcattta aaggtatagg ctgttgagct ctttggcata
     2281 aatcttgcca aaatggcgga atcaatgcca ccgctaaggg ccagtgccgc cttaccgtct
     2341 gagactgctt tttctacttg ccgtttcagc gactcttcaa gctgaaagct gttttcaata
     2401 ggttcgcgct caaaatccgg cttaaagagt ctgggtttta cgccttcttt gaaagtcttg
     2461 gtttcatcgg taattgttct gtacattaaa aatgagctgg aacaaaaccg tttgtcggca
     2521 gtcaccgtat cacctcattc gctgcgaagc aaattaagcg cctgggtaat ctttgtggat
     2581 gatactcctt gcgtataggg gaaatatata atttttatgc cggcctcgga aaattccttt
     2641 tcgtagttct cccacttttc ggttccgtac caatcgtcgc ccacaaaaag aattgacgcg
     2701 cccagctttt tgcacatggt tagcttatcc atgtcgtact gagcaaccgc ggcgtcaaca
     2761 tacttaatgc tgcgcacaat ctctatgcgg tcatggaaag gaataaccgc gcgcttgccc
     2821 ttgtatgtaa ccagctcatc ggcggttact cccacaatca gcttgtcaca catccccttt
     2881 gcgtttttaa gcaggttcag atgacctata tggaacaagt caaaaacgcc tgctgtgtag
     2941 cctatggtca tcctttatca ttctcctttt tccaataata aatcccgtgg aacggttgcc
     3001 ttttgtcttc gggaggcagc tgcatatagt cgccgtattt aagtcttaag ctctcatcgt
     3061 aatttccttg gcaccaaagg taagaatctc tgaatttgag tcggattctc ttctccaaat
     3121 cctctttttt attcaaatcc ctttcagcct tgccccagat tccgacagaa acatattcgg
     3181 aagtttcata aggatatctt tttgcctctt ctgcaatggc gtctctgatt tttttgtagt
     3241 caattttaga aaaagctata tatatcattg tatgagcaat tcttttgagg aaattgctgc
     3301 ttttaatttt tttaatatca aaaatgctga aataaaggca ctgctttaag atggcaagct
     3361 ttctgaaatg cttttgtctt tcctccccgg actcgggcag accgtcaatg gggaatatgt
     3421 ctataaaggc gctttgcatt cccagctcat ctctaagctg tcgctcctcc attacggttc
     3481 tggtatctac catctttata aagggctggg tgtactggca gaccgcgtaa tactcccacg
     3541 gagagttctt ggtaagctct ataaagcgct cataatcggg tcttggcata gccacatcca
     3601 catcgtcatc ccaagggata aaaccctgat ggcgcactgc tcccaaaagg gtgccgccgt
     3661 aaaggtagta gcgcaggtcg ttttcatcgc agaactctgc gaatgccttc agcatatcgt
     3721 ataagacatc ctgcagttca ctcaaggtga aaagtttttt atcgctcaag ctatacatcc
     3781 ttcttcctaa ccagttctat caagatattg gggttcaagc cttttaacaa aagggtgttg
     3841 ttgcgtctta tgggctttat tatataacct aatataaagt ttgtgaaaat ctgcgttatc
     3901 aaagccgtga gcgccgctcc gttgatgccc caaaggggga tgaatatgag atttagaatc
     3961 acatttgtaa gagcgccgca aaggtttata atccaaagaa ctttgtattt gccctccgcg
     4021 agtatccaga tgttgcggac cgagcccatg tgggaaaaag caagatacca aacgattatg
     4081 cgcagggcgg gaattgacgc gaggtactgc tctccgtaaa gtatttttac cacaagctcg
     4141 gcgaaaagcg caatacctgc ggactgcaca agggcaaagt atatgattat ggagtaaagc
     4201 cttgatacat tcttttcgaa tgaagcgtgg tccgcctttt tgctctcaaa aatcacgggt
     4261 ctgaatgagt cgataatagc ggcgaaaaca aagtatgtca ctcccgcgga ggctacggcg
     4321 gcggaataga gtcccactgc cttttcatct atcatgagct tcagcatgac tcggtctatt
     4381 tgaccgaaaa caacaaccat catgcttgat acgatatagt atctgctttt tgaaaaaagg
     4441 agctttgcgg tatccggcga gaatgacagc ttttgtgtgc cgagtctttt atatatgaaa
     4501 agcagcacaa ccgcaataat agcaaaatca agggcatttg aaacagcaaa ccagtaaatg
     4561 cttttctttg aagcgagaag gaaaatctta tacagcgcgg tgattatata ggctgcgaac
     4621 attgtaagcg aagtgtattt tgacatcagc tttgcctgga accagtactg cagcgcttca
     4681 acagcctgga atatcaagag tatactgtaa agaacgcaga ctatgattgt atctttttcg
     4741 cccgcattga caattgtcgt aaacgcaaag acgcccagta tgcaaaaaag gcttgagact
     4801 agggacattg ttagggctgt acccaaggtt tcgccttcct tttgaggctt tatgactatt
     4861 tcctgcacaa gtattgagtt aagtcccaaa tacattaaag gcacgaaaaa cgctaccaaa
     4921 gaggaggcat agttaatcag tccgtaattg gagggaccga gataccttgc cgtaaacatt
     4981 gaaacgacaa ggccaattac agactgcgct actttacagg caattatcca tgcgctgttt
     5041 tttacgacct tgtttttata ttgcataatc caccgcttat ataaatccgt ctctataata
     5101 ttgtctgtac tgttgacttg aatgcggcgc agtacagaag aacatcaatt ctcgaaacac
     5161 aacagcataa ttataccatt attctgttaa aggtcaatta tttaattttt tagtgttttt
     5221 gataggatta aacctatttt ataatggcca gcataaaaaa tcaaattatg agtagtttcg
     5281 ctattaaaag ttaaaattaa aagttgctta gtgttaattt gtcaatagag aaaaatattt
     5341 tttttaaatt ttgattgcat tgggattgac aattaatcta aaatgtgtta taaatttaac
     5401 aaaaaatggg ataatttctt tcattgggtg aggcaatgag gcctttcacc ctgtttttat
     5461 agagtattgc agaatattta atgcaaaata agttatgcgt tctcgttttt aatcgggagg
     5521 aggcaaactt aatttatagg tgggaggaaa caatatgact ttttatgaaa gcctgatggt
     5581 tggtctgttt tgttttacag gcgttttttt ggttcttgtt gtcttgaatt tgctgattag
     5641 gctcttttcc gcagttttgg gctctgccgc aaagaaaact tccagcgcgg caaaaccgga
     5701 gcctcccgcg gcgacctcac agcaaaccgt aaaaactccc gaaacaaaag attcgagtaa
     5761 ccaagtagag cttatagatg ttgatgacgc taccgctgcc gttattatgg cgattgtaag
     5821 ccacgaaacg ggaatccctc tttcaaatct tgaatttaag tatattaagc tcgttcagta
     5881 gatactgact ttagtctgcc aaatctcagg gcaggcgcta aaagagacaa ggaggcaaat
     5941 ttatgtatat agaggctata cgaaagattt gggaaatgtc cggatttgcg gcgcttacat
     6001 ggcagcacgg cgttatgata cttatcgcat gcgtactgat gtattttgcc atagtcaaaa
     6061 aatttgagcc gttgctttta ctgcccattt cattcggaat gctgcttgcc aacctgccgc
     6121 tggcgggact tatgggcgct ccgatgggag aagaagcggg cggactattg tattatctgt
     6181 atcaaggtgt taaactcggc atttatccgt cgctgatttt tatgggaata ggcgcgatga
     6241 ccgactttgg tccgctgatt gcaaacccct caagcttgct gcttggagcg ggcgctcagt
     6301 tcggaattat tgtggcattc attattgcgg ttgctttggg ctttgacccg aaagccgccg
     6361 catcaatcgg aatcataggc ggcgctgacg gtccgacggc aatttacctt acctcaaagc
     6421 tctcccctga ccttttgccc gcaattgcaa ttgccgcata ttcttacatg gcgctcatcc
     6481 cgatgattca gcctccgata atgagactgc tcactaccga gaaagagcgc aaaatcgaga
     6541 tgaaacagct gagggaagta tcaaagctgg agaaagtatg cttccccatc attgtaacta
     6601 ttctctgcgt tcttctgctg ccctcggtcg ctccgcttat cggaatgctg atgctgggca
     6661 acctgttcag ggaatcgggc gtagttgaaa gactttcgga cactgctcag aacgcgctta
     6721 ttaatattgt aaccattttc cttggagtta ccgttggagc tacctcctcc gctgagagat
     6781 tcctgaggct tgaaacaata aagattattg ctctcggact tgcggccttt gcgttcagta
     6841 ctgtgggcgg aattcttttg ggcaagctga tgtgcgtact tacaaagggc aaggttaacc
     6901 ccttaatcgg ctccgcgggt gtgtccgcag ttcctatggc tgcaagagtt tctcagattg
     6961 agggacagaa ggctaacccc agaaacttcc tcttaatgca cgccatgggc ccgaatgttg
     7021 cgggagttat aggctcagcc gtggcagcgg gtatactgct tgctttgttc ggataaaaat
     7081 aaaccccatt cgtttttgcg aatggggtgt ttttatgttt agcgatagaa aatccagacg
     7141 agaatatatc cagtcattac ggcggcgagg gaaaagggta cgctgatttt cataaactcg
     7201 gcagacttta cctcgtagcc gtttttcttt aagattccga gacctgcaat attcgcggat
     7261 gctccaatgg gtgtaagatt gccgccgagt gtcgctccca cgataagtcc gaaataaaga
     7321 acagtcgcgt ttatgcccat aaggtttgca atgcctgcgg ttacgggcag catggttgct
     7381 atatagggaa tgttatcgat aaacgctgag aagagaaccg atgcccaaac caaaagagtg
     7441 tacataagga atacattgct gccgctaagg tttacaaaga tctcgctgat ttttgtaacc
     7501 acgcccgctt cggtaatacc gctgataact atgaacagac ccgcaaggag aaggagcgtg
     7561 aagtagtcga tttccttcat tgtgcgtttg aatgcctcaa ggttccttga gcgaattgtt
     7621 tcgcgaatca ggcccgcaat cataaagcca atgcagataa gtccgtttgt gatggcgggc
     7681 tttttgtcag cggggatgaa agaggcgaga atcaggaaaa cgactgtgcc aacaagcaga
     7741 acggaaggga agtaatcctc caccctggtt ttgtccttaa catctatgct gtaattatcc
     7801 ttgcgcagta cataaaacaa tacaagcgtc gcggcaagag ccccgatctg aactatccag
     7861 aaaaggcccg gcttgccttg atatactata aaatcaagga aatccatatt catgtggctg
     7921 ccaagaagaa ttgaggttgt atctccaacc agcgtagcgg cgccttgaag gttagaggct
     7981 atggcaatgg aaattatgct ctttacaggg gaaatattaa gttttttagc tatgttcaga
     8041 gcaacgggag caaccataag taccgtcgcc acattgtcca caaaggcgga gataaggcct
     8101 gcgaacaagg aaagggatat aatcgcccat tttacattgg gcatcttcat tataatcaaa
     8161 tcagcaagaa gggcaggcat tttggactct ataaacagcg atacaacgcc cattgtgccc
     8221 gctatcatca ttatgacatt ccagtcaacc gaggcaaaaa ccttgcttgc gggcagtatg
     8281 ccgagtacga caaaaagcgc ggctgatgcg agcgcaatat agggtctgta tttcggcagt
     8341 gccattaaaa gcacataggt taaaagaaat aaaactagcg cttgaaccaa ttgttacatc
     8401 ctttctaaaa ctgatagtaa aaacactcga acattataac acccgtgtcc aattttgtct
     8461 aacggaaaaa acaaaaatgt tgtataaact cactaaagga tttttgtacg gagctgataa
     8521 attacggcgt tatgaggcga atcagctcgg gcgggtaatc tggaactgcg ccgtattttg
     8581 taacaacata atcgctgagg agaatcgccc tctcggcgca ttcttcggtg ggaacgccgc
     8641 ttaggaagtt cgctataaag caggcggaaa agctgtcccc cgcccccacg ccggacaccg
     8701 ccttggattt cggctttgag gggctgtgga acttatcctt tcccgcgtca tagattaaag
     8761 cgccgtcttt gtcgagagta agaaggataa gctttattgc gtacttttcg gaaaggctgc
     8821 ggcatgcgta cttttgtgct ctttcggtgt cattattgtc agcgtccgca aaaccgttat
     8881 taattaaatg ccgcagctcc tctctgttta ttttgagaat ggaagcggtt ttaagcgcaa
     8941 agtctattat ctcatcggaa taaaagctct gtctcaaatt caaatcacaa aaaatatgct
     9001 caaagctgcc tgtgtttaca aggcacttga gcgtctcaaa ggatgtatcg cttcttaaag
     9061 caagggtgcc gaaataaaag aggttaaact cccctgccgc cgacctgttt acaagttcgg
     9121 tatatggctc tatataatcc cacgcggcgg gctgagtaag gtcatagccg ggtctgccgt
     9181 tttcgtcggt ggttacaatg caggtgcctg tgggataggg caccgttcgg acaaaatcgg
     9241 tttttacgcc caaactgtca atgattttca atgtctcctc gcccaagtcg tcatccccaa
     9301 tggcggtgag gctgtaagag ttaataccaa gcttggctaa atgggcgcag aaattaaagg
     9361 gagctccgcc gatttccctt gtgtttccga atttatccca aagcacttct ccgaatgcga
     9421 gtaccttcaa tttcatctct ccaaagcatt ttattttcta taaaaatagt attacaaact
     9481 ttggtcagta ttcaagcgca aaagaggaaa ttattcccta acagaaacaa agcctcccgt
     9541 ttgggaggct tcgcttttta aaaacgcagt taactagatg aaaaggtcgt agtagttctt
     9601 gataaagtag ctgacaaaag cgagaatcat agccagatac tcaaagagcc tcattattga
     9661 ttcgaggagt gaatccgtct tgccgggctc tgtgaccgta aactctgctg tcgcaattcc
     9721 gccgaccgac tcaatgccta tggtgtatgt gcctgtcggg agagttttaa ggtattcttc
     9781 cttgagggta accactatac cgtcctctga ctcggctgct tcatagtttg actcgtcaag
     9841 actctcgccg tttaacctaa cgccgacaaa atcgccgaat gccgcgctgg acttaaacac
     9901 gattgtgcct gcgctcttgg gattccaaac actgcccttg cctttagtaa taacgggcgg
     9961 gagcatcggg atatctactg ttcttgtctc accgcagaca aggcaggtgt aaagcattgt
    10021 gcccttactt ctcgcagtgg gctggaggat tactgtgcct tcatcccatt cgtggggctg
    10081 cttttcgcag agaacagtgc agccgtcgtt tgcgcactcg tgccaatgac cttctgcgtc
    10141 gtgatggcac tcgcccgtaa agtcatgctc ggttgcggcg gataagaccg ctccgcatac
    10201 tgagcagagg tcgggcgttg tgcagtcgcc gtcgtcttcc tcgggtatgt gagctgctat
    10261 atcggttttt gctccgcagc ctgcacactc atgccagtga tgagtttcgt catgtctcca
    10321 ctcggcgctg aaactgtggg gagccactct ctcctcgccg cagatgccgc agtcggcgtc
    10381 gcagtcgctg tcgtaagtat gctctgcgtt tactgtaatg gtcaaaacct ttgtattgcc
    10441 ggcattgtca tatacgataa tcaaatgttc accgggttcc gtaatgacat atcttccgtc
    10501 ggcggcagta atctcataag agttatccgt tatggcgcca aagttttcat cataaacgct
    10561 gaactcagct ttgccgcagt ataccgctcc gtcttctatt cctgtgactg tcggggcagt
    10621 ggcgtcaata ataattccgt tagacgcaaa gtagtctgca ttgcccgcgt tgtcggtgac
    10681 tttaacatag accactgccc tgcggtcagg atttatcagg aacatgtcat catatacatt
    10741 ccagtcggtg acatccttta cctcattaag agtcagaata tccgaagaaa ggtaatactc
    10801 tacagacttt acgcctgaaa ggctgtcaat actctgaatt attatataaa gggacgcatc
    10861 gaaatagtga gaaccaaaac tgatttcgcc aaagttttta tctatgggaa tatctttaaa
    10921 aatgtaaaag actgagattt gtgcagtcgg ggcttctttg tcgatcttac agctttcgta
    10981 tgcaatctga gaaatcagac ccgaagcctc atcctttaca taaaattggg ttgtatagtg
    11041 tccctggtca ttgtttacaa ggtaatcata ccatatggcg tttgcgttat tgactcttga
    11101 cagcttgtag ccgggcatag cggttattac aaagtctgaa ttggcccatt cgtttgttgt
    11161 ggtggtgtag tggatgccct tgactgctgc aaatgagttt tcaatgctgc ccgttacatt
    11221 agtgggctgg attaattcat agttatcagc gtgagtgcct atgaggctga agctttcttc
    11281 aaagacaacg gggatgtttt tgccggcatt cacgcttgta aatctcgcaa ttggataggg
    11341 ctccgcgaca gcgacattgt cgccgcttat taagcccgaa aggttaactg actcgatttg
    11401 agcatcgtta agtccgttat actgctttgt cttgatgaca acactgatgt taagcggcgc
    11461 cttggtgatt cttgcggtta cgggagcggg ctgagaggca agcaaataat ttcccgcgtc
    11521 ggttcccgtt aaggcgaaat cggtgaaata tacagtccaa gtgcctgcat ttttgttctg
    11581 gaagaaagca gtgccgcgca ttactcctac agagtcgccg ctgactacgc cgttcaaaac
    11641 gggctcgccg ataatttcgg cggcatcggt accgtcatag accttgtcag agacggatat
    11701 gccagaaatt gttaccgttt tcttgttgac ggtaaaggat ttatcaaccg ttatggggtt
    11761 atagtcgttg tctcccgctt tggtggctct tattgtgact gtacccgcgc ctgtgattct
    11821 gactttattt ccgtttataa tccgggcggg accttcaagc accgtaaaac tgatattgcc
    11881 gctgcccgaa ccgccgattg cgcccaaggt gaaatcagcg tcgccgtatg tcttgactgc
    11941 ggggaagttc tcgtcaaatg aaagctctgc ctgaatgccc ttccggaatg tatatggaac
    12001 tgcggcgctc acggcggagt tatagctgct gtcgcccgaa tactcaacag tgaaggcata
    12061 ggaatcgtaa gaacctgcag gcgtaaactg aacggactga cctacgggta cggtggcgat
    12121 aacatcggtg cctgctttaa atgtaagtgt gcctgtcgcg tccgcgggga ggactgctga
    12181 aagagttaca cttcccggtc tggtctgatt ctggggtgaa acttgcagtt caattgtcgg
    12241 agctcccttt tcaaccgaaa ggttgatagt cacatcagtg agcttatcgc ttccttcata
    12301 aggctccgcc aatatgtatc ttctatgatt gtagctgagg gttagcgtat actcgccgag
    12361 aggaagtgta ttgagaaagc ttgcgttcag ctgaattctg tcataattgt cacttgtttt
    12421 ggaaagagtg tagtccgcat ttttttggag caccctgtct cctattccga tttcttttag
    12481 cgcgtgacct tggtatccgg cgctggcttt gttatatatt gtgaagggcg catagttcag
    12541 agaacctttt ttgtgagtta tcgaatcagt gtttgatgtg acattgccga aaagagtaaa
    12601 tatatcagaa ctgataattc ttacatttcc tgaagggt
//
LOCUS       DAONVQ010000051        12605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3840_length_12605_cov_4.720478, whole genome shotgun sequence.
ACCESSION   DAONVQ010000051 DAONVQ010000000
VERSION     DAONVQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3840_length_12605_cov_4.720478"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..3800)
                     /gene="smc"
                     /locus_tag="PLD98_04620"
     CDS             complement(210..3800)
                     /gene="smc"
                     /locus_tag="PLD98_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQA05565.1"
                     /translation="MYLKSIEMQGFKSFPDKTVMRFEKGITAVVGPNGSGKSNIADAV
                     KWVLGEQSTKSLRGNKMEDVVFGGTAKRRPQGYAEVVLRLDNSDNAIKSDILDGEKLG
                     SEIIVSRKYYRSGESEYRINGNIVRLKDIHQLFMDTGLGPDGYSIVGQGRIESLVSAK
                     SSERRDMFEEAAGISLYRHRREEAQRKLEQTEENLVRLRDILLELESRVGPLKTQSEK
                     AKKFLRLAEEKREIEIGIWLNIIEKSSRQLKEYSDKEQIAKAQHEEILAELENIIRRS
                     EEADSKTKEITLKLDELQKSISKFEEAITSFESQAAVAKNSLFHLESSRERLGKELSQ
                     ENITKEVLTEQKTELLSQIEELKAEIEKKKSEISSITSELLTLKDKDDSFTQQFNALT
                     NQLTEIERNLSEQRIAESSALSSAEEINKRKQELIESINDKKQQIKNLEEELKKAKAA
                     KEECIENLESAENTVKGYAIKVRNASAAAENANKKLSDEQLKADRYRSRIKLLSDMEK
                     NMEGYAGSVKAVMKRAHDGFLTGIHGPLSQLIKVESEYETAIETALGAALQNIVTDNE
                     NDAKKAINYLKESGEGRVTFFPLTSIKGRTLEEKGLENSRGFIAIASELVEYDKIYGE
                     IIVSQLGRTVVVEDIDSAIEMARKYSHRFKIVTLDGQVINAGGSMTGGSKIRSGGFLS
                     RSNEMESLKSRLAEIEKNLEALKQEAKQAQELYAREVAAFEGAQGEQLRAKEALIYSE
                     NQLSITESKYNDAVSALEATEQELQRLEQRLKISNDIALKAKCEIERLNAEKVGIQEK
                     INLISSDRENLISASRAIDERIAAINIDLAALNNELNSKANAAADIECRITAHDEKAE
                     GLYWEINELKVKESELKNSIETYEANALELKAKITSIKAEMDKLIEERENAEKEVYSL
                     RALERQKTAEKENISGELIRLQERIDSLTKEQADLENKLFEEYGLTKREAERLEIKID
                     SPQAASRRLSELKSQIKALGNVNLAAIEEYKEVSERYEFLSTQIKDVEKSKDELGRLI
                     YDLTQKMTEIFKEKFDLINMHFAEAFKQLFGGGDAKLILEDEENMLECGIEITAQPPG
                     KSVKSLSLLSGGEKGLCAVSLLFAMLKVSPSPFCIFDEVDAALDDINVIRFASYVRSI
                     CDKTQFILITHRRGTMEEADLLYGVTMQEEGVSKLLELKTAEMAKSLGLE"
     gene            complement(3823..4833)
                     /locus_tag="PLD98_04625"
     CDS             complement(3823..4833)
                     /locus_tag="PLD98_04625"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQA05566.1"
                     /translation="MASRKQDKHINVALFVPHEGCPNDCMFCNQKAISGKTERLTAAD
                     VALAAETAIGSGKDTTGGEIAFFGGSFTAIDKDYRKELLDAAKPYIENGSFKGIRIST
                     RPDFIDDEILMLLKEYGVTAIELGAQSMVDKVLKANCRGHLSEDTRRASELIKSYGFE
                     LGLQMMTGMYADTDEGTLYTADEFIKLRPATVRIYPTIVLKNTMLAELYYKKLYEPQT
                     LGEAIEICAQLLLKFNAANIKVIRLGLHSGGGVEPDYIAGPYYPAFGELCESRVYFKK
                     AADMLKDYPKGKYQLIVNKSEISKMIGQRRENLIKLAEMGYECRVLGSEDIIPYEIKI
                     SR"
     gene            complement(4835..5503)
                     /gene="rnc"
                     /locus_tag="PLD98_04630"
     CDS             complement(4835..5503)
                     /gene="rnc"
                     /locus_tag="PLD98_04630"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQA05567.1"
                     /translation="MNDLIKKIGYEFKDESLLIRALTHSSYANESGGKNESNERLEFL
                     GDSVLGFITAEFFFSREKDLPEGELTKLRAAAVCEKALSGFANKIGLGEYLLLGKGEI
                     NSNGKERPSILADAFEALIAAIYLDGGIENAKKFVTQFLEDFSAEYYVINDAKTILQE
                     IVQKNPGELLEYVLVNESGPDHAKCFEVEVRLNANVIGRGKGRSKKQAEQLAAKEALS
                     LMGL"
     gene            complement(5522..5758)
                     /locus_tag="PLD98_04635"
     CDS             complement(5522..5758)
                     /locus_tag="PLD98_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQA05568.1"
                     /translation="MIFERVAPILAEHFNVPEEDISEEMTFEELGADEIDLIDLATIL
                     EDEFDIEIHEDEIKDIETVEDLVSLIRTAVELSA"
     gene            complement(5765..6775)
                     /gene="plsX"
                     /locus_tag="PLD98_04640"
     CDS             complement(5765..6775)
                     /gene="plsX"
                     /locus_tag="PLD98_04640"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HQA05569.1"
                     /translation="MKVIVDAFGGDNAPIEILKGSRLAKDEYSVDIILVGNKAEIEKA
                     AKENDIDISGMEIVESGSIITAYDSPTSVIKSKQDSSMAVGLSLLADGKGDAFVSAGS
                     TGALVVGGTFIVKRIKGIKRAAIGTLMPSAENPFILLDAGANVECTSEFLNQFAIMGD
                     IYMRRIMNVSNPRVALANIGEEETKGTKLYQDAYKLMKESNYNFIGNIEARDIPYGKA
                     DVIVADGFTGNIILKTYEGAAGALAGMIKAMFKKNALTMLSALLVKSGIDEFKSKMDY
                     KEFGGAPLMGICKPVIKAHGSSDARAFKNAIRQAKMFAENNVISEIENNLPKTNTNSN
                     QE"
     gene            complement(6842..8155)
                     /gene="trmFO"
                     /locus_tag="PLD98_04645"
     CDS             complement(6842..8155)
                     /gene="trmFO"
                     /locus_tag="PLD98_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HQA05570.1"
                     /translation="MSNAKTAVVIGAGFAGCEAAKQLSKRGIKVKLYEMKPKKFSPAH
                     TSENFAELVCSNSFKAMRLETAAGLLKKEMEHFGSISLKFAYKSQVPAGGALAVDRDL
                     FSKLVTEEIESDENIEVIRDEVTSIPEADAVIVAAGPLVSDALSKELEKLAGGFLSFY
                     DAAAPIVTAESIDMNSAFYASRYDRGGDTDYINCPLNKEEYETFYNELINAETAELHS
                     VDLNKEVFEGCMPIEVLAKRGPDTIRFGPMKPVGLRDPKTGQRPWAVLQLRKENAEGT
                     MYNLVGFQTNLKFGEQKRVFSLIPALKNAEFVRYGVMHRNTFINSPKALNENLSFKSA
                     TNVFAAGQITGVEGYMESAACGIVAGINAANYLNGKTHLSLPLETMMGALINYTVRST
                     SSDFQPMGASFGLLPPLPVQIRDKKERYRALAERSLKILSGINMD"
     gene            complement(8148..10280)
                     /gene="topA"
                     /locus_tag="PLD98_04650"
     CDS             complement(8148..10280)
                     /gene="topA"
                     /locus_tag="PLD98_04650"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQA05571.1"
                     /translation="MSNLVIVESPAKAKTIKKYLGPDFEVVASMGHVRDLYPSTLSVD
                     VKNNFAPKYTIIKGKRPVIKQLKDLAAKSEHIYLATDPDREGEAISWHLATLLKIKPE
                     DELRVRFNEINEQSVKKGIASPTKIDRNLVNAQQARRILDRLVGYSLSPFISQKIRRG
                     LSAGRVQSVAVRLIVDREEEINAFVPEEYWSIDAKLTAPPGRKVFKAAFVGDQTGKVK
                     IATKEQADEYLKRLDGAVFTVSDVKKGVRRRQPAPPFITSTMQQEASKRFGFSAQKTM
                     KLAQELYEGIEVKGNGVTGLITYMRTDSLRISDQARNDGINFIRNTYGENYIPEKNRV
                     FKTRAGAQDSHEAIRPTQAELTPDRVKDSLTADQYKLYSLIWKRFIASLMADCIQDTV
                     KVEIKAASEADSSNERYCLFSISGYTIRFDGFTVLYDEVSEDDEEDKIIPPVTKGDVL
                     KLKELIPQQHFTQPPARYTEASLIKTLEETGVGRPSTYATILHTIVNRGYVVKEGKSL
                     KPTELGIATTNLLKEWFPKIVNTKFTARMESKLDDVEEGKTDYIEMLHGFYGEFEQTL
                     TKAKQELVGVKIQLEEDKTDIVCEKCGRNMVVKVGRFGKFLACPGYPECKNTKPYVQK
                     TAATCPKCGGDVITKRSKSGHAFYGCSNYPDCNFMTWDQPLEEKCPQCGSSLFKARGG
                     VIKCHKEGCGYEQKAQRKKKEKSEESDE"
     gene            complement(10309..11562)
                     /gene="dprA"
                     /locus_tag="PLD98_04655"
     CDS             complement(10309..11562)
                     /gene="dprA"
                     /locus_tag="PLD98_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQA05572.1"
                     /translation="MSPKEYWIWLQYALGAGARVDEILAQYGSPEKIYYAGSRDWRLS
                     GVFKADTISRLLKKSPSETYSVIKECEENGWHIITPDDEYYPDGFKNLTNFPLVLYVW
                     GDPEVLKHQVPISIVGTRYASVYGKKIATALAASLSKAGALIVSGGAKGIDSCAHIGA
                     LNAKGKTVAFLGCGLKSNYLIENLELRKLIAKNGAVVSEYTPQTEPTRFAFPIRNRLI
                     SAMSLGTVVIEAGEKSGSLITARLALEQGRDVFAVPGDIVSSNFKGTNRLLREGAKPV
                     FSALDVLEEYVFEFADRLNLENTGELIIDNSVSTDIPKVSYESNTAARKSVKINRTAE
                     KQQNSAQITFESAAKENPQSKPTAPEGLSECAMQIFGFLSDTPISTEEILSKSSFPSS
                     EVLTALTELEFSGAVKVFPGGLFSV"
     gene            complement(11559..12341)
                     /locus_tag="PLD98_04660"
     CDS             complement(11559..12341)
                     /locus_tag="PLD98_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQA05573.1"
                     /translation="MTELTSKTNPKIKHFVKLRSDASYRREHGQFVLESLRLCVDAFL
                     SGTEIVEVFVTNKALNQYKNELSGLISAAKTFLITDEISDKMSDTASPQGVFCICKML
                     DKEAPCNKIEVNGKYIASDSVQNPSNLGAIARTAEALGISGIIVGGGCDIYNPKAQRA
                     AMGSLLRLPVISVDNLPAYLTGLKEKGFSVYGAVPDSDALPVTAVDFNKPCVCVIGNE
                     ANGLSDAVLSVCTDRITVPMKGRAESLNAYSAAAIIMWEMVR"
     gene            complement(12474..>12605)
                     /locus_tag="PLD98_04665"
     CDS             complement(12474..>12605)
                     /locus_tag="PLD98_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQA05574.1"
                     /translation="YSTFINGLKKANINLNRKMLSEIAVSDPEAFTALTEKAKAALQ"
BASE COUNT         2998 a         3086 c         2701 g         3820 t
ORIGIN      
        1 cccataacaa gtatttcttc aagctctgta tataaatcct cgcttaaagt atcaaaggaa
       61 tctaataaat catcaatcgc gcccgacatt ttgtctcggg tcttttttag tccgaaatta
      121 atcttgctga aaatacccac caaaccacac cttttaaatc tgattactta attatagcat
      181 acaaatattt gccatacaat attcagaaat cactcaagac cgaggctttt tgccatttcc
      241 gctgttttca gctcaaggag ctttgaaacg ccctcctcct gcattgtaac gccgtagagc
      301 aaatcagcct cttccatggt gccgcgcctg tgagttatga ggataaactg agttttatcg
      361 caaatgcttc tgacatagct cgcaaagcgg atgacattta tgtcgtcaag agcggcgtca
      421 acctcatcga agatgcagaa gggagaagga gaaaccttaa gcatagcaaa gaggagcgac
      481 actgcgcaaa gacctttttc accgccggaa agcagggaaa ggcttttgac cgatttgccc
      541 ggcggctgcg cggtgatttc aatgccgcac tccagcatat tctcctcatc ctcaagaatg
      601 agctttgcgt cgccgccccc gaaaagctgc ttgaacgctt cggcaaagtg catattgata
      661 aggtcaaact tctccttaaa gatttcggtc atcttctgcg ttaaatcata aatcagtctg
      721 cccagctcat cctttgattt ttcaacatcc ttaatctgag tgcttaaaaa ctcatagcgc
      781 tcggaaactt ccttgtattc ttcgattgcg gctaaattta catttccgag agccttaatc
      841 tgcgacttca gttctgaaag cctgcgggat gcggcttgcg gagaatcaat ttttatctca
      901 agacgctccg cctctctctt tgtaagaccg tattcctcaa agagcttgtt ttcaaggtcg
      961 gcctgctctt ttgtgagtga gtcgattcgc tcctgaagtc ttatcagctc gcctgagatg
     1021 ttttcctttt cagcggtctt ttgcctttca agagctctta ggctgtaaac ttccttttcg
     1081 gcgttctccc tttcctcaat cagtttgtcc atttcagcct tgattgaggt gattttcgct
     1141 ttaagttcaa gggcgtttgc ctcataggtt tcaatgctgt ttttaagctc cgactcttta
     1201 accttgagtt cgtttatttc ccagtacaag ccctctgcct tttcgtcatg ggcggttata
     1261 cggcattcta tatctgcagc ggcatttgcc ttggagttca gttcattatt taaagccgca
     1321 aggtcaatat tgattgccgc aattctctcg tcaatcgccc tgcttgcgct tataaggttt
     1381 tctctatcgg aggatataag gttaattttc tcctggatac caaccttttc ggcattgagc
     1441 ctttctattt cacactttgc ctttaaagca atatcgttag aaatcttgag cctttgctca
     1501 agacgctgaa gctcctgctc agttgcttca agagctgaaa cagcgtcatt gtactttgat
     1561 tccgtaatgg acagctgatt ttcgctgtaa atcaaagcct cttttgctct gagctgctct
     1621 ccttgagcac cttcaaaggc ggcaacctcg cgggcataaa gttcctgagc ctgctttgct
     1681 tcttgcttta gagcctcaag gtttttctct atctcagcaa gccttgattt aaggctttcc
     1741 atctcattgc tgcggcttaa gaatccgccg cttctaatct ttgagccgcc cgtcatggag
     1801 ccgcctgcgt ttattacctg accgtcaagg gtgacgattt taaagcggtg agaatacttt
     1861 cttgccatct caatggcaga gtcgatatcc tcaaccacaa cggttctgcc aagctgagaa
     1921 actatgattt ccccatagat tttgtcatat tccacaagct cgctggcaat agcgataaag
     1981 cctcttgagt tttcgagacc tttttcctca agagttctgc ctttgattga tgtaagcggg
     2041 aagaaggtta ctctgccttc gcccgattcc ttaagataat ttatagcctt cttagcgtcg
     2101 ttttcattat ctgtgactat gttctgaagg gcggcgccaa gtgcggtttc aatcgcggtt
     2161 tcatattcgc tttcaacctt aataagctgc gaaagcggtc cgtgaatgcc tgtcaggaac
     2221 ccatcatgcg cccttttcat gacagcctta acgctgcccg catagccctc catattcttt
     2281 tccatatcgg aaagaagctt tatgcgggaa cgatatctgt cagcctttaa ttgctcgtct
     2341 gagagctttt tattggcatt ctcggcagct gcagaggcgt ttctgacctt tatcgcatag
     2401 ccctttacgg tattttcggc agattcaagg ttttcgatgc actcctcttt agccgctttt
     2461 gcctttttca gctcttcctc aagattctta atttgctgct ttttatcatt tatagattca
     2521 atcagttctt gtttgcgctt atttatctcc tcagcggagg aaagagcaga gctttcggca
     2581 atcctctgct cggaaagatt tctttcaatc tctgtaagct gattggtaag agcgttaaac
     2641 tgctgagtaa agctgtcgtc cttatctttt aaggtaagca gctctgaggt aatgctgctt
     2701 atttcactct ttttcttttc aatctcggct ttaagctcct caatttgact taaaagctct
     2761 gttttttgtt ccgtaagaac ttcttttgtt atgttctctt ggcttagctc tttgcccaat
     2821 ctctcccttg aggattccaa atggaaaagg gagtttttcg cgaccgcagc ctgagattca
     2881 aaggaggtta tagcctcctc aaactttgaa atggactttt gaagctcgtc cagctttaaa
     2941 gttatctcct ttgtcttgct gtcagcctct tcgctgcgtc ttatgatatt ttcaagctct
     3001 gcaagtattt cctcatgctg agcttttgcg atttgctctt tgtcggaata ctctttaagc
     3061 tgccttgagg atttttctat gatattaagc catatgccga tttcgatttc tcgcttttct
     3121 tcggcaaggc gcaggaattt ctttgccttt tcgctttgag tctttaaggg acccaccctt
     3181 gactcaagct ctaaaagaat atctctgagt cttacaaggt tctcctcagt ctgctcgagc
     3241 ttcctctggg cctcctcgcg gcggtgccga tataaagaaa tgcccgccgc ctcctcaaac
     3301 atatcccggc gctcagagga ttttgcggaa acaagactct ctattctgcc ctgaccaaca
     3361 attgaatagc cgtcgggacc gagacccgtg tccatgaaaa gctgatgaat atctttaagc
     3421 cttacgatat tgccgttaat tctgtattcg ctctcgcctg agcggtagta tttcctcgat
     3481 acgataatct cgctgccgag tttctcaccg tctaaaatat cggacttaat ggcattgtcg
     3541 gaattgtcaa gccttaatac aacctcagca tagccctgag gacgccgttt tgcggtaccg
     3601 ccgaaaacaa catcctccat tttatttcct ctcagggatt ttgtagactg ctcgccaagg
     3661 acccacttaa cggcgtcggc tatattgctc ttgcctgagc cgttagggcc aacaacagcg
     3721 gtaatgccct tttcaaagcg cattacagtc ttgtcaggaa aggatttaaa gccttgcatt
     3781 tcgattgatt ttaagtacat actcaagtcc tttactgcta aactaacggg agatttttat
     3841 ttcatacgga attatgtctt ctgagcctaa aacacggcac tcgtagccca tttcagcaag
     3901 ttttattaag ttttctcgcc tctgccctat catctttgat atttcggatt tattaacgat
     3961 aagctgatat ttacctttag ggtaatcctt tagcatatca gccgcttttt taaagtagac
     4021 tctgctttcg caaagctcac caaaggcagg ataatatgga cccgcaatat aatcgggctc
     4081 cactcccccg ccggaatgaa gtccgagcct tataacctta atatttgccg cattaaactt
     4141 aagcagaagc tgagcgcaaa tctctattgc ttcgcccagt gtctgcggct cgtacaattt
     4201 cttataatac agctccgcaa gcatggtgtt tttaaggaca attgtcgggt atatccgcac
     4261 ggtagcggga cgaagcttta taaactcatc ggctgtataa agagtgcctt catctgtgtc
     4321 ggcgtacatg ccagtcatca tttggagccc aagctcaaag ccatagcttt ttataagctc
     4381 cgaggccctg cgggtatcct cggacaaatg gcctctgcag tttgccttta ataccttgtc
     4441 caccatgctc tgagcgccaa gctcaattgc ggtgacacca tactctttta agagcattag
     4501 aatctcatca tctataaaat cggggcgtgt tgagattctg atgcccttaa agctgccgtt
     4561 ttctatgtag ggctttgcgg catctaaaag ctcttttctg taatccttgt caatagcggt
     4621 aaagctaccg ccaaaaaaag caatttcgcc gcctgttgtg tccttgccgc tgccgatggc
     4681 ggtttcggcg gcaagcgcta catcagcggc tgtaagcctt tcagttttgc ctgatattgc
     4741 cttttggttg cagaacatgc agtcgttcgg gcagccctca tggggcacaa aaagcgccac
     4801 atttatatgc ttgtcctgtt tacggcttgc catattacag ccccataagg ctcaaagcct
     4861 ctttggcggc aagctgttca gcctgctttt tgcttctgcc tttgcccctg cctattacat
     4921 ttgcgttgag tcttacttca acctcaaagc atttcgcgtg gtcgggaccc gattcgttga
     4981 ccagtacata ttcaagcagt tcaccgggat tcttttgaac gatttcctgc agaatcgtct
     5041 ttgcgtcgtt aattacatag tattcggcag agaaatcctc caaaaactga gtaacgaact
     5101 tctttgcgtt ttctatgccg ccgtcaagat aaattgcggc gattaacgcc tcaaaggcgt
     5161 ctgcaagtat tgagggacgc tccttgccgt ttgagttaat ttcgcctttg cccaaaagga
     5221 gatattcccc cagaccgatt ttgttggcaa agcctgagag agccttttcg caaacggcgg
     5281 cagcgcggag cttagttagc tcgccctcgg gcaaatcctt ttcgcggctg aagaaaaact
     5341 cagcggtgat aaagccgagc acggagtcgc ccaaaaattc aagcctctcg tttgactcgt
     5401 ttttgccgcc gctttcgttc gcatatgagg aatgtgtgag agcccgtatt aaaaggctct
     5461 catctttaaa ctcatatcct atttttttaa ttaaatcatt cattgtaatc tcttcttcct
     5521 gctatgcgga gagctccaca gcagtcctga tgagcgacac aaggtcctct acggtttcta
     5581 tatctttgat ttcatcctca tggatttcga tgtcgaattc gtcctcaagg attgttgcaa
     5641 ggtcgataag gtcgatttcg tcggcaccca gttcttcaaa agtcatttcc tcgctgatgt
     5701 cctcttcggg aacattaaaa tgctcagcta aaataggtgc aactctctcg aatatcatta
     5761 aaaatcactc ctgatttgaa ttagtatttg ttttgggaag attgttttca atttcagata
     5821 ttacattatt ttcggcaaac atttttgcct gtctgattgc gtttttgaat gccctggcgt
     5881 cggatgagcc gtgagctttg ataacgggct tgcagatgcc cataagcgga gctccgccga
     5941 attctttgta atccattttg ctcttaaact catcaattcc gctctttaca agcagagccg
     6001 agagcatggt gagggcgttt ttcttgaaca tagcttttat catgcctgcc aaagcgccgg
     6061 cagcgccctc ataggtcttt aatattatat ttcctgtgaa tccgtctgct actattacat
     6121 cagcctttcc gtaaggtatg tcgcgcgcct ctatattgcc gatgaagtta tagtttgact
     6181 ccttcatgag cttatatgcg tcctgataga gctttgttcc ctttgtttct tcctcaccta
     6241 tgttagcaag ggcaacgcgg ggatttgaaa cattcattat ccttcgcatg taaatgtcgc
     6301 ccataatggc aaactggttt aggaattcgg acgtgcattc aacattcgcg ccggcgtcaa
     6361 ggagtataaa ggggttttca gcggagggca tcagtgttcc tatagcggct cttttgatgc
     6421 ctttaattct ttttactatg aatgtaccgc ctacaaccag tgctcccgtg ctgcctgcgg
     6481 atacaaacgc gtcgcctttg ccgtcggcaa gcagagaaag tcccactgcc atagatgaat
     6541 cctgctttga cttaattaca gaggtaggag aatcgtatgc ggtaattatt gaacccgact
     6601 ccactatctc catgccgctg atgtcaatat cgttttcctt ggcagccttt tcaatctctg
     6661 ccttatttcc gacaagaatt atgtcaacgg agtattcatc ctttgctaag cgagaacctt
     6721 ttagtatttc tatcggcgca ttgtcgccgc cgaaagcgtc aactataacc ttcatagccc
     6781 tactccctat tattcattga aaattatgcg gatatgcagt aacttattac agcttgtttt
     6841 attaatccat atttattccg ctgagaatct taaggcttct ttcggccaat gccctgtacc
     6901 tttccttttt gtcccgaatt tgaacgggca agggaggcaa aagaccgaag cttgcgccca
     6961 tcggctggaa atcggacgag gtgctgcgca cagtatagtt tatcaaagca cccatcatag
     7021 tttcaagagg tagacttaaa tgagttttgc cgtttaagta atttgccgcg tttatgcccg
     7081 caactatgcc gcacgcggca gactccatat aaccctccac tcccgtaatc tgaccggccg
     7141 caaagacatt cgtcgcggat ttaaaggaga gattttcatt taatgccttg ggcgagttta
     7201 taaaggtatt cctgtgcatt acgccgtacc ttacaaactc ggcgttttta agggcgggta
     7261 ttaatgaaaa aacccttttc tgctcgccga acttgagatt tgtctggaaa ccaacaagat
     7321 tgtacatggt accctctgcg ttttcctttc tgagctgaag aacagcccag ggtctttgac
     7381 cggtcttagg gtccctgaga cctacgggct tcatgggccc aaagcggatt gtgtcgggtc
     7441 cgcgctttgc caaaacctca atgggcatgc agccttcaaa aacctcttta tttaaatcca
     7501 ccgaatgaag ctcggcggtt tcggcattta taagctcatt gtaaaaagtc tcgtattcct
     7561 ctttatttaa tgggcagttg atatagtcgg tatcgccgcc tctgtcatag cgggaggcat
     7621 agaaagcgct gttcatgtct atgctttcgg ctgtgacaat tggcgcagcg gcgtcgtaaa
     7681 agctgagaaa accaccagcc agcttttcca gctctttaga cagagcatcg gacactagcg
     7741 gacccgcggc aacgattacc gcgtcagcct caggaataga ggttacctca tcgcggatta
     7801 cctcgatatt ctcatcgctt tcgatttcct cagtgacgag ctttgagaac aaatccctgt
     7861 ccactgccaa ggcgccgccc gcgggaacct gagatttgta ggcgaattta agggagattg
     7921 agccgaaatg ctccatttcc ttctttaaaa gccctgcggc ggtctctaaa cgcatagcct
     7981 tgaaagaatt tgagcataca agctcggcaa agttttcgga agtatgggcg ggtgaaaatt
     8041 ttttcggctt catttcataa agttttacct ttatgcctct ctttgatagc tgctttgcag
     8101 cctcacagcc tgcaaaaccc gctcctatta ccaccgctgt tttagcgtta ctcatcggat
     8161 tcctcagatt tttccttttt cttgcgctga gccttctgct catatccgca gccttccttg
     8221 tggcacttta taactccgcc ccttgcctta aagagggatg agccgcactg ggggcacttt
     8281 tcctcaagag gctggtccca ggtcataaag ttgcagtcgg gataattgct gcatccatag
     8341 aaggcgtgac cgcttttcga tctttttgtt ataacatcac cgccgcactt ggggcaggtg
     8401 gctgctgtct tttgaacata gggcttagtg tttttgcatt cgggatagcc ggggcatgca
     8461 aggaatttgc caaagcgtcc gactttgaca accatattcc gtccgcactt ttcgcagaca
     8521 atgtcggtct tgtcctcttc aagctggatt tttacgccca caagctcctg ctttgcctta
     8581 gtaagggtct gctcaaactc gccgtagaag ccgtggagca tttcgatata gtcggttttg
     8641 ccttcttcca catcgtccag cttggactcc attctcgcgg taaacttagt atttactatc
     8701 ttcgggaacc attcttttaa cagatttgtc gtggcaattc caagctctgt gggctttaat
     8761 gacttgccct ctttgaccac atatcccctg tttacgattg tgtgtagaat ggtggcatat
     8821 gtgctgggtc tgccgactcc tgtttcttca agagtcttaa ttagtgatgc ctctgtatat
     8881 cttgcgggag gctgggtgaa gtgctgctgc gggataagct ctttaagctt tagcacatca
     8941 ccctttgtga cgggaggaat tatcttatcc tcttcatcat cctcagaaac ctcgtcgtaa
     9001 agaacggtaa aaccgtcaaa gcggattgta tagccgctga tagaaaacag gcagtagcgt
     9061 tcgtttgagc tatccgcctc acttgcggcc ttaatttcga ccttaacggt atcctgaatg
     9121 cagtccgcca taaggcttgc gataaaacgc ttccagataa gcgaatacag cttatactgg
     9181 tcggcagtga gtgaatcctt tactctatcg ggtgtaagct cagcttgagt gggccttatg
     9241 gcctcatgac tgtcctgagc tcccgccctg gttttgaaaa ccctattctt ttcagggatg
     9301 tagttttcgc cgtaggtatt tctaataaag ttaattccgt cgtttctcgc ctgatctgag
     9361 attctaagag agtcggttct catataggta ataagacccg taactccgtt gcccttaacc
     9421 tcaattcctt cgtacagctc ctgagcaagt ttcattgtct tttgagcgga gaaaccgaaa
     9481 cgcttagatg cctcctgctg catggtggag gtaataaagg gcggggcggg ctgcctgcgg
     9541 cggacgccct ttttgacatc cgatacagtg aatacagctc cgtcaagacg ctttaaatac
     9601 tcgtccgcct gctctttagt ggcgatttta actttgcctg tttgatcacc gacaaaagcg
     9661 gctttaaaaa ccttgcgtcc gggaggggct gtgagctttg cgtcaattga ccagtattct
     9721 tcgggaacaa aggcgttaat ttcttcttcc ctgtcaacaa tgagccttac ggcaacgctc
     9781 tgaactcgtc ctgcggaaag tccccttctg attttttgag agataaaggg gctgaggcta
     9841 tagcccacaa gacggtcaag aattcttctt gcctgctgag cgttgaccag attgcggtca
     9901 atcttagtag gcgacgcaat gccttttttt acgctttgct cgttgatttc gttaaacctg
     9961 accctgagct catcctcagg cttaatcttt aacaaagtag caagatgcca agagattgcc
    10021 tcgccctcgc ggtcagggtc cgtagcgaga taaatatgct cgctttttgc ggcaaggtct
    10081 ttaagctgct taataacagg gcgcttgccc ttgatgattg tatatttcgg agcgaaatta
    10141 ttcttaacat caacgcttaa ggttgacgga taaagatctc tcacatgtcc cattgaagcc
    10201 acaacttcaa aatcgggacc caaatacttc tttatggttt tcgccttggc gggcgactcc
    10261 acgattacaa gattagacat atatcaaccg tcctataaaa agagttagtc atacagaaaa
    10321 caaaccgcca gggaagactt taaccgcgcc tgagaactca agctccgtca aagccgtcag
    10381 tacctctgag gagggaaaac tgcttttgct gagtatttct tccgtgctaa tcggagtatc
    10441 gcttaaaaat ccgaaaatct gcatcgcgca ttcagacagt ccttctggtg ctgtcggctt
    10501 agactgggga ttttcttttg cggcggattc aaaggtaatc tgcgcagaat tttgctgttt
    10561 ttccgccgtt ctgtttattt tcacagattt tcttgcagca gtattagatt catatgagac
    10621 ttttggaata tctgtgctta cggagttatc tataataagc tcccctgtgt tttcaaggtt
    10681 tagcctgtcc gcaaattcaa aaacatattc ctcgagcaca tcaagcgctg aaaagacagg
    10741 ctttgcgcct tcacgaagga gcctgtttgt ccctttaaag tttgagctga caatatcgcc
    10801 gggaaccgca aatacatctc tgccttgctc taaagcaagt cgggcagtaa ttaaagagcc
    10861 gcttttttcg cccgcttcga taacaaccgt tcctaggctc atggcggaaa taagcctgtt
    10921 tctaatgggg aaagcaaatc ttgtgggctc cgtttgagga gtgtattcgc taacaaccgc
    10981 gccgtttttt gcaataagct tcctcagctc caaattctca attaaataat tgcttttaag
    11041 cccgcagccg aggaaagcaa ctgttttgcc ctttgcgtta agcgcgccga tatgagcgca
    11101 gctgtcgatt ccctttgcgc cgccgctgac aatcagcgca cctgcctttg ataaagaagc
    11161 ggcaagagct gtggcaattt tttttccgta aacgctggca tagcgagtgc caacaataga
    11221 aatcggaacc tgatgcttta aaacctcggg gtctccccac acataaagca caagagggaa
    11281 attggtgagg tttttaaagc cgtcgggata gtattcatcg tcgggagtta ttatatgcca
    11341 accattttct tcacattcct tgattacgga ataggtctca gacgggcttt ttttaaggag
    11401 ccttgatatg gtatcggctt taaaaacgcc tgaaagccgc caatccctgc ttccggcata
    11461 ataaattttc tcaggcgagc cgtattgggc aaggatttca tctacccttg cgcctgcgcc
    11521 aagggcatac tgaagccata tccagtactc ctttgggctc acctgaccat ctcccacatt
    11581 attattgcgg cagcagaata agcgtttaag gattccgctc tgcccttcat aggcactgta
    11641 attctgtcgg tgcagacgct gagcaccgcg tcggataatc cgtttgcctc atttccgata
    11701 acacatacac agggcttatt aaagtcaacc gctgtgacgg gcagagcatc gctgtcggga
    11761 accgcgccat atactgaaaa gcccttttct tttaaaccgg ttaaataagc gggaagattg
    11821 tccacgctga taacgggcag acgcaaaagc gagcccatag cagcccgctg cgcctttgga
    11881 ttatatatgt cgcagccgcc tccgacaata atgccgctga ttccgagagc ctcagcggtc
    11941 cttgcgattg cccccaaatt agaggggttt tgaacgctat ctgacgcaat atatttgccg
    12001 ttgacttcta ttttattaca gggggcttct ttgtcaagca ttttacaaat acaaaaaacg
    12061 ccctgaggcg acgcagtatc ggacattttg tcagaaattt cgtcagttat caagaatgtt
    12121 tttgcggcac ttataagccc tgaaagctca ttcttatact gattcagagc tttatttgtc
    12181 acaaatactt cgacaatttc cgtgccgctc aaaaaagcgt caacacagag ccttagactc
    12241 tcaagcacaa attgaccgtg ctcgcgcctg taagaggcgt ctgagcgcag cttgacaaaa
    12301 tgctttattt tcggatttgt ctttgaagta agctccgtca tattaatccc ttttaatttc
    12361 tttttgatta tggaaagtat aattcaaaaa catggaaaaa tcaacaagca gaaaaaaaag
    12421 ggaataccaa taatggcatt cccttagctt ttcaaaatat tacattgatt agcttactgc
    12481 aaagctgcct ttgctttctc ggtaagagcg gtgaaagcct cagggtcgga aacggcgatt
    12541 tcggaaagca tctttctgtt aagattgatg ttagccttct taagaccgtt gataaaggta
    12601 gaata
//
LOCUS       DAONVQ010000052        12596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_3843_length_12596_cov_4.607926, whole genome shotgun sequence.
ACCESSION   DAONVQ010000052 DAONVQ010000000
VERSION     DAONVQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12596
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3843_length_12596_cov_4.607926"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PLD98_04670"
     CDS             complement(<1..628)
                     /locus_tag="PLD98_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05575.1"
                     /translation="MKKSVLKVFSVALVITLFAAMFATPAADLQYAAAETPVVEAPAA
                     AEPENEAQDAPTAAFMSVQAASETETSTLGNPIADVLEKDKAGFSRVQKWFYNLVQSI
                     VDTIIKGFVTLLPVPTSWVSKEDFVSENFYPGMDEFLTEPADGAVWNVGYASASLVEG
                     LDFTKNNFYMGGYFHYPPLKAKGVYDDLRVRAFAINDNSGRGTALFAVL"
     gene            complement(828..1382)
                     /locus_tag="PLD98_04675"
     CDS             complement(828..1382)
                     /locus_tag="PLD98_04675"
                     /inference="COORDINATES: protein motif:HMM:NF027438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4830 domain-containing protein"
                     /protein_id="HQA05576.1"
                     /translation="MFVFSVKSLKLKLIAIIAVVLIACVVFIYFAKRDKPASAGGAIN
                     YSAANEDERKAFLAQFGWETSGEPIEVVEVIIPAEFDEVYENYNKIQLEQDLNLKPYA
                     GYRVKRWTYEITNYKGYENKPGYIRANLLVYDGRVIGGDICSVELDGFMHGFKNPKAA
                     SEQTTGESQTTAEAATKKAKTGSA"
     gene            complement(1512..2072)
                     /locus_tag="PLD98_04680"
     CDS             complement(1512..2072)
                     /locus_tag="PLD98_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05577.1"
                     /translation="MNVDQMYDRIEELLDEGKPSLIGGTVKVNADEIRALIKEIRLKA
                     PEELAQARRIAAERKDILAAANEAAEAKIKEAEKIAEKIVSEHEITLKAKAVANEIIT
                     EAKAQAAEIIEKARASSNDIIDNAQKWAMDMRSSASEFVENIMAESEEILEEGYRDFA
                     NNLEKVRNARRQLKNASIKKGVLPKE"
     gene            complement(2073..2567)
                     /gene="coaD"
                     /locus_tag="PLD98_04685"
     CDS             complement(2073..2567)
                     /gene="coaD"
                     /locus_tag="PLD98_04685"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQA05578.1"
                     /translation="MRRIAVCPGSFDPITLGHLDIISRTAKMFDKVLVVVMSNYRKPK
                     GTFTVAERMDFIKRCTADMPNVIVDSYDGLLAEYVESRGAIAIVKGLRAVSDFEDEFK
                     QALTNKILRPNVETIFMVSSSEYMFLSSSVVKEVCGYGGDISTFVPEVIREDIIKRIG
                     KGGI"
     gene            complement(2597..3142)
                     /gene="rsmD"
                     /locus_tag="PLD98_04690"
     CDS             complement(2597..3142)
                     /gene="rsmD"
                     /locus_tag="PLD98_04690"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HQA05579.1"
                     /translation="MTMMRVITGIAKGRRLFTLEGNDVRPTSEKVKEAIFSIIQFEIE
                     GRRVLDLFAGSGQLGIEALSRGAESAVFVDSSRAAVDVIKKNLEVSGLYDKAVVKNTN
                     ALTFLETYDSFFDIAFVDPPYSQGLAAAALERLPMCMSESGIIICETRFNEELPTDLG
                     SLKLYRTYKYSKTSVSVYRPK"
     gene            3704..4522
                     /locus_tag="PLD98_04695"
     CDS             3704..4522
                     /locus_tag="PLD98_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05580.1"
                     /translation="MILIEDCLKEFEKKISAEIEAQKVRQKRINEIFANAYNLDGIVS
                     TEPSEKDIGIKMKDIRSAVSDLISYAVGCVFGRYSLDFEGLIFAGGEWDEEKYKTFPP
                     VKENIVVLAGRKYGLKNDLSLLVSEFIKTVFGEEIYEENMKFIAKALSGDSYIGSPLA
                     VLDSYFLKDFYKEHVRAYSGRPIYWAVSSGRAKAFFGYIYYHRIDQDTIKYIKAFSLS
                     AAEKAEEKKKNELYAFIERIDNIINLNPSIDFDAGIMYNYNNLSDFLAGLDRQF"
     gene            4970..5986
                     /locus_tag="PLD98_04700"
     CDS             4970..5986
                     /locus_tag="PLD98_04700"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA05581.1"
                     /translation="MKKLTKVFAVLLAVTMVFTLFACAKKDSDGETSAAGGDAKKFKI
                     AIIQPMEHLSLNEIRDTIVAELKALGYTDDKAEIILKNANGDMSLLPSIYNNLITEKV
                     DIYVPIATPTAQAAKAAVSDKPIIFSAVSDPVKAGLVTDIEKPAENITGVSDPIPVDQ
                     TLAFAKELVPSIKTIGFIYNVGEANSVSAIERAKKYCDENGIAYKEANVAVTGDIQQA
                     VASIIDEVDAFFTPDDNTVASGMNTYAQVALEKKKPIFCGADSMVKEGGLATVGINYV
                     TLGKLTARMVDKVLKGASVQDTPVEYVKDYAKVINTDVCEELNITLPANTEGFVLVKR
                     ENNN"
     gene            6066..6962
                     /locus_tag="PLD98_04705"
     CDS             6066..6962
                     /locus_tag="PLD98_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA05582.1"
                     /translation="MALLLTGAIQLGMIYAIMAMGVFISFRILNVPDLTVEGSFTLGV
                     AVSAVVATKGHPVLALFCALLAGAAAGLVTSFLHTKIRIQTILAGILTMTSLYTINLA
                     IMGGRANISLFASETVLTLLEKSTGLSSGMVKTIVTIAVTAIVFALLSLFFKTRFGLK
                     IRATGDNEEMVRASSINASVTKCAGLMLGNACVALSGAMLCQYQKSADISLGAGMVVV
                     GLASVIIGEALVGKRNVILGILSVVVGSVAYQIIIAIALKVRLLPAYGLKLISACIVA
                     ISLTLPVVRDEIKKVRRGKKNV"
     gene            6967..7779
                     /locus_tag="PLD98_04710"
     CDS             6967..7779
                     /locus_tag="PLD98_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA05583.1"
                     /translation="MLKLCNVTKVFHPNTVNENRAIDNLSFELKKGDFVTILGSNGSG
                     KSTMFNLIAGSLLADSGEIYLEGENITLVPDYIRARKIGRIFQDPMKGTAPSLTIEEN
                     LALAYSRKSKSKIFSINKEDRKVFVELLSMLGLGLENRLKTKIGLLSGGQRQAVTLLM
                     STIADPSLLLLDEHTAALDPNTAVKILEITKELVEYSKTSTMMITHDINAALSIGNRT
                     IMMDSGRIILDISGEERKNMTVESLLKLYKKESKKELSNDRILFSAGDMGEE"
     gene            7847..8671
                     /locus_tag="PLD98_04715"
     CDS             7847..8671
                     /locus_tag="PLD98_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQA05584.1"
                     /translation="MSLPVAIQLYTLRDEMEKDVKGTLQKVKEFGYDGVEFAGLYGYS
                     AAEMKALLEEIGLMPVSAHVALKDILADMENVIATYKEIGCEYIAIPYLPGDRRPEAG
                     DFDSTIADIRKIGEECKKQGIILLYHNHDFEFAKLDDKYVLDVLYDSIPADLLQTEID
                     VCWVNVAGVDPAEYVLKYTGRSPVVHLKDFVMKSRQKPAKLYELIGIEDDEKTEEEDF
                     SFMPVGYGVQDIPGIINAAEKAGAKWLVVEQDRPAKNNTPLESAKLSRDYLKTLGY"
     gene            complement(8719..9378)
                     /gene="phoU"
                     /locus_tag="PLD98_04720"
     CDS             complement(8719..9378)
                     /gene="phoU"
                     /locus_tag="PLD98_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQA05585.1"
                     /translation="MRSRFEQQLAELHESLIEMGAMIERGIAQATKALINKDIEAAKH
                     VISSDDAVDSKEKDIESMCLKLLLSQQPVAKDLRQISTALKMITDMERIGDHAADISE
                     LCVYMAEDYDDFHLNEYIPEMARTTIKMVTDSIEAYVKKDLVLAESVIATDDIVDNLY
                     LEVKKSLIELIHEDVKKGELAFDLLQIAKYYERIGDHAENIAEWVIFSITGMHKKEQV
                     L"
     gene            complement(9378..10127)
                     /gene="pstB"
                     /locus_tag="PLD98_04725"
     CDS             complement(9378..10127)
                     /gene="pstB"
                     /locus_tag="PLD98_04725"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HQA05586.1"
                     /translation="MNCFTVEGLNIYYGSFHALKNINIELEHKKVTAFIGPSGCGKST
                     FLKTLNRMNDLVEGCRIEGTIKLFGENIYDKMDVNILRKRVGMVFQKPNPFPISIYDN
                     IAYGPRTHGIKEKHILDEIVEESLKKAAIWDEVKDRLKKSALGLSGGQQQRLCIARAL
                     AVSPEVLLMDEPTSALDPISTGKIEELIIALRDKYSIVIVTHNMQQALRISDNTAFFL
                     MGELIEYSATEKMFTMPKDRRTEDYITGRFG"
     gene            complement(10124..10954)
                     /gene="pstA"
                     /locus_tag="PLD98_04730"
     CDS             complement(10124..10954)
                     /gene="pstA"
                     /locus_tag="PLD98_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HQA05587.1"
                     /translation="MSKKQKRTEKFLAAMTYGAFIFTFSVLVFIIAFILFKGVPHLKP
                     SLFSLKYTSENVSLMPALISTLIMTAISIAISVPLGVFSAIYLVEYAKRGNKVVNLIR
                     LTTETLSGIPSIIYGLFGLLFFVTYLGWGFSVLAGSFTLSIMILPLIIRSSEEALKAV
                     PDSYREGSFGLGAGKLRTVFRVVLPAAIPGIFAGIILAVGRIVGETAALIYTAGTVAQ
                     VPKDLLQSARTLSVHMFALSSEGLHTGEAYATAVVLLILVVVINTVSAMVAKRIGGKA
                     "
     gene            complement(10951..11787)
                     /gene="pstC"
                     /locus_tag="PLD98_04735"
     CDS             complement(10951..11787)
                     /gene="pstC"
                     /locus_tag="PLD98_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HQA05588.1"
                     /translation="MNKAKEKIMSIVFLIAALTSIFAVALICLFLIVNGIPAMAKIGL
                     FDFLLGKEWAPSDVPAEYGILPMILGSLYVTAGAVLIGVPIGILSAVFLAFVCPKKLY
                     KKVKPAVDLLAGVPSVVYGFFGMAVLVPITGYSMLTASLLLGIMILPTVIGLAESALR
                     AVPTQYYEGSLALGAGHYKSVFSVVLPAAKSGLLAAVVLGIGRAIGETMAVIMVAGNQ
                     ARIPHSLFKGVRTLTANIVIEMGYASELHRDALIATGVVLFVFILIINLSFSALKRRS
                     DK"
     gene            complement(11774..12595)
                     /locus_tag="PLD98_04740"
     CDS             complement(11774..12595)
                     /locus_tag="PLD98_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQA05589.1"
                     /translation="MLDKIEKGEFKADKEIAVFSREDGSGTRTAFTEIFNIVEMGENG
                     TRKDRTTKEAVIAKQTDVLMSGIASDRYSIGYLSLGSVNKTIKLISIDGVYPTAETVK
                     NGTYAVSRPFIIVTKKEISPATQDFINFMLSKEGQAVAAESYISAVDNAPEFNSSMPS
                     GKVVVAGSSSVTPVMEKLKEAYIEINPNAIIEIQLSDSSSGIQGVIDGTCDIGMSSRH
                     LKDSEKAVLDQHTIALDGIAIVVNKNNPINNLSSEQVKAVFKGEITRWRELIEQG"
BASE COUNT         3475 a         2885 c         2774 g         3462 t
ORIGIN      
        1 caagaacagc gaaaagggca gtgcctctgc ctgaattgtc attgatggcg aaggctctta
       61 ctctgaggtc gtcataaacg cccttagctt ttaaaggcgg atagtggaaa tagccgccca
      121 tgtagaagtt gttctttgta aagtcaagtc cttcaacaag ggatgcggat gcgtagccca
      181 cattccaaac agcgccatcg gcgggctctg tcaggaattc atccattccg ggatagaagt
      241 tctcggaaac gaaatcctct ttgctaaccc atgatgtggg aacggggagg agggttacga
      301 agcccttgat tattgtgtca acaattgact gaacgaggtt gtagaaccac ttttgaactc
      361 ttgagaatcc tgccttgtcc ttttcgagaa catcggctat ggggttgccg agagtggaag
      421 tttcagtttc gcttgcagcc tgaaccgaca taaatgccgc ggtcggcgcg tcctgtgcct
      481 cgttttcggg ctctgctgcc gcgggagcct cgacaacggg agtttctgct gctgcatatt
      541 gcaagtcagc agcgggagtt gcaaacattg ctgcgaacaa ggtaataaca agcgcaaccg
      601 aaaaaacctt taaaacagat tttttcataa aatctcatac tccttcattt tcaatcagta
      661 caaaataaat ttagcataaa tactacataa tatcaatagt taattgactt aattaagcaa
      721 agctaacaaa ttatttttgc tttgcggcag aataaaaatg atttattttt aatgaaaaat
      781 aaaaatgggc agagtatttt ctctgcccgc tttgctttta aaatgcatca tgccgaacct
      841 gtttttgcct tttttgtggc ggcttcggca gtggtttggc tttcaccggt agtctgttct
      901 gaggcggctt ttggattttt aaagccgtgc ataaagccgt caagctcaac tgaacagatg
      961 tctccgccta tgaccctgcc gtcgtaaacc agcagattcg ctcttatgta gcccggtttg
     1021 ttttcatagc ccttgtaatt cgtaatctca taggtccatc gtttgaccct gtagcctgcg
     1081 tagggcttta agttaaggtc ctgctcaagc tgaatcttgt tgtagttttc gtagacctcg
     1141 tcaaattcgg cagggatgat aacctctaca acttcaatcg gttcaccgct agtttcccag
     1201 ccgaattggg caagaaaagc cttgcgttcg tcttcattgg ccgctgagta attaatcgcg
     1261 ccgccggcgg aggcaggctt atcccttttg gcgaaataaa taaacacaac gcaggcaatc
     1321 agaacaacag cgataattgc aataagtttc agcttcaatg attttaccga aaatacaaac
     1381 atgtccgcac ctcgcaaata ttttgcttct caccgaaaga taccggtcaa ctaactaata
     1441 tgcgaggtcg gacaaaaaaa tgtttgttta attaaaactt gttcgatatt ttatgttcta
     1501 atctttatgt tctattcttt aggtaaaacg cccttcttaa tggatgcatt tttgagctgt
     1561 ctgcgagcgt ttctaacctt ctcaaggttg tttgcgaaat ccctgtagcc ttcctcaaga
     1621 atctcttcgc tttcagccat tatgttctct acaaattcgc tggcgctgga cctcatatcc
     1681 atagcccatt tctgagcgtt atctataata tcgttggagc tggctcttgc tttctcgata
     1741 atctcagcag cctgtgcctt ggcttcggta attatctcgt tggcaaccgc ttttgccttt
     1801 agggtgattt catgctccga aacgattttc tcagcgattt tctcagcttc tttgattttt
     1861 gcctctgccg cttcgtttgc tgctgcaagt atgtccttgc gctccgctgc tatccttctt
     1921 gcctgggcaa gctcctcagg ggctttgagc cttatctcct taatgagagc tctgatttcg
     1981 tcagcattaa ctttaacggt tccgccaatc agtgacggtt tgccttcgtc aagcaattcc
     2041 tctattctgt catacatttg atcgacattc attcaaatgc ctccctttcc gattctttta
     2101 attatgtcct cgcgaataac ttccggaaca aatgtagaga tgtcgccgcc gtatccgcag
     2161 acttctttaa caacgctgga gcttaaaaac atatattcag agcttgagac catgaaaatt
     2221 gtttctacat taggtcttaa tatcttattt gtaagggcct gtttgaattc atcctcaaaa
     2281 tcggacacag ccctgagtcc ctttactatt gcgatggcgc cgcggctttc aacatattcc
     2341 gcgagcagac cgtcgtatga gtcaacaatt acattcggca tgtcggcggt gcatcttttg
     2401 ataaaatcca ttctttccgc aacggtaaat gtaccctttg gttttctgta attggacatt
     2461 acaacgacca aaaccttgtc gaacattttt gccgttcggg agataatatc aagatgaccg
     2521 agggtaatag ggtcaaagct accggggcaa acagcaatcc ttctcattac ttaaaactcc
     2581 ttaaactctg cgcgatttat ttgggtctgt atacgctgac agatgttttt gaatatttat
     2641 aagtcctgta aagctttaaa gagccgagat cggtcggcag ctcctcatta aagcgggtct
     2701 cgcaaatgat tatgccgctt tcggacatgc acatgggaag tctctccaaa gccgcggctg
     2761 caagtccctg tgagtagggc gggtcaacaa acgcgatgtc aaagaaagaa tcataggttt
     2821 caaggaaggt aagggcgttg gtgtttttaa caacagcctt atcataaagt cccgaaacct
     2881 caagattttt cttaatgaca tcaacagccg cacgggagga gtcaacaaaa accgcgcttt
     2941 cagctccgcg gctaagcgct tcaatgccaa gctggcccga gcccgcaaag aggtcaagaa
     3001 cccttcttcc ctcaatctca aactgaataa tgctgaaaat agcctcttta actttttcgg
     3061 aggtaggtcg gacatcattg ccctctaagg taaaaaggcg tctgcctttt gcaattcctg
     3121 taattactcg catcatggtc accttggctt ttttgagccg gttcttctgc aaattttctg
     3181 cataagttaa ggttattatc ccactttttg tttgattttg tcaagttgtc attgaaatta
     3241 aagtattaag atttttcaaa ttaaattatt ccattaaaaa gcagaattaa ttcttttaag
     3301 cctttaattt agccttgaat ttaattaaat taaaaataaa acaacaaaaa ggctgtcgtt
     3361 tgacagcctt ttcgtttaga ctttatcggc gattagcagc agtgattgtt actatcggca
     3421 aggagtaaga gtaaaaggat gagaatcaag cagctattgt ttccggataa gcaattcata
     3481 aatcgttcct cctttcgtgc tttcatgaac atgatatgtt aaagaataaa ttgcgttaca
     3541 gtttagaggg actaaattat atttttttaa ttgaaatttg taattttaaa gcgatttttg
     3601 cgctcttaca atgctgaatc caaaaaactt gactcaaaaa atgatattcg gtacaatcct
     3661 tttaaggttt tggcttaaaa ttattgaaaa aagcggttat gaaatgattc ttattgagga
     3721 ttgcctaaaa gaatttgaga aaaaaatcag cgcggaaatt gaggctcaaa aagtaaggca
     3781 gaaaagaata aacgaaattt ttgcgaacgc ctacaatctc gatggaattg tcagcaccga
     3841 gccttccgaa aaagatattg gcataaaaat gaaggatata aggtcagcag tctctgacct
     3901 tatatcctat gctgttggct gcgtttttgg ccgctattct ttggattttg aaggtttaat
     3961 ttttgcgggc ggggagtggg atgaagaaaa atataaaact tttcctcccg taaaagaaaa
     4021 catagtagtt ttggcgggca gaaaatacgg gctaaaaaat gatttaagcc ttttggtctc
     4081 agaatttata aaaacagttt tcggcgagga aatatacgaa gaaaacatga aatttatcgc
     4141 aaaagccctg agcggcgatt cttacattgg ctcgccctta gctgtgcttg acagctactt
     4201 tttaaaagat ttctacaaag agcatgtaag agcctattcg ggcagaccaa tatactgggc
     4261 tgtttcaagc ggcagggcaa aagcgttttt cgggtatatt tattatcaca gaattgacca
     4321 agacacaata aaatacatta aggcattctc cttatccgcc gcggaaaagg cggaagagaa
     4381 gaaaaaaaat gagctttatg catttattga gcgaatcgac aatataatta atctaaaccc
     4441 gagcatcgat tttgacgcag gaataatgta taattataat aacctgtctg atttcttagc
     4501 cggcttggat aggcagtttt aataaaaatt ggtattttta taaattattg ttgacaaagg
     4561 aaattctgca tgctattatc agttcacaat tgaatatcag ctattaaacc gatgaagcgg
     4621 aagttaagct gacatatgcg cgcccagaga gtgagcggtg gtgggatgct catcgagatg
     4681 caggtcagtg caaatggtcc gctgagggcc gagtgaaagg ctgcatgcag ccgagtatcg
     4741 aggacgtgag tctgcgttaa ggaacgggga tgtgttggca tcccgctgag tgggtcgctt
     4801 cacggcggcc aaataaggtg gcaccgcagg tattttatga cctgtcctta tgagagtaag
     4861 gataggtctt ttctttttgt ccaacagcaa aaagagaaac ttatcgattt aagtacgcgt
     4921 acacaaatcg gccaatcaac tcaacaaaat cttaaaggag aggtacatga tgaaaaagtt
     4981 gacaaaggtt tttgcagttc tcttggcagt tacaatggtg tttactcttt ttgcttgcgc
     5041 taaaaaggat tccgacgggg aaacaagcgc agccggcggc gacgcaaaga aattcaaaat
     5101 cgctataatt cagcccatgg agcacctttc actgaacgaa atccgtgaca caattgtggc
     5161 agagcttaaa gctctcggat acacagatga caaagcagaa ataatactta agaacgccaa
     5221 cggcgatatg tccctgctgc cctcaatcta caacaacctc atcactgaaa aagtggacat
     5281 ttatgttccc atcgcaaccc ccacagctca ggccgcaaag gctgcggtaa gcgacaagcc
     5341 cattattttc tccgcagtaa gcgaccctgt taaggcaggt cttgtaacag acattgaaaa
     5401 gcccgctgaa aacatcacag gcgtttccga cccaatacct gttgaccaga cccttgcctt
     5461 tgctaaggag cttgttccct caatcaagac aatcggcttt atctacaatg tgggcgaggc
     5521 aaactccgtt tccgctattg agagagcaaa gaaatactgc gacgagaacg gaatcgctta
     5581 caaggaggca aatgttgcag taaccggcga cattcagcag gctgtagcat ccatcattga
     5641 cgaggttgac gccttcttca ctcccgacga caacaccgta gcatccggca tgaacaccta
     5701 tgctcaggta gctctcgaga agaaaaagcc cattttctgc ggcgcggact caatggttaa
     5761 agagggcggt cttgcaacag taggcattaa ctatgtaact ttaggcaagc tgacagccag
     5821 aatggttgac aaggttctca agggcgcgtc cgtacaggat actcccgtag agtatgtaaa
     5881 agactatgca aaggttatca acaccgatgt atgcgaagag cttaacatca ctctgcctgc
     5941 caataccgaa ggctttgtac tcgtaaagag agagaacaat aactaaaact ttaagaaagc
     6001 actctatcat cagggcagaa tttctgccct ggtgatagaa attttagatc atcagaggta
     6061 gttttatggc tttattatta accggcgcga tacagcttgg aatgatatac gccatcatgg
     6121 ctatgggcgt ttttatatcg ttcaggatac tcaatgtccc cgatcttacg gtcgagggtt
     6181 cgtttactct tggcgtcgct gtatcggcgg ttgttgcgac aaagggacat cccgtgcttg
     6241 ctcttttctg cgcgcttctt gcgggagcag ccgcgggttt agtcaccagt tttcttcata
     6301 caaaaatcag gattcaaaca attcttgcgg gcatactcac catgacctcc ctttatacaa
     6361 taaaccttgc cataatggga ggcagggcaa acatctccct ttttgcctcc gaaacggtgc
     6421 tgacgctttt ggaaaagtcc acaggacttt catctggcat ggttaagaca atcgtcacca
     6481 ttgccgtaac cgctattgta tttgcgcttt tatctttatt ctttaagaca aggttcggac
     6541 ttaaaatcag ggcaacgggc gacaatgagg agatggtaag ggcatcctct ataaacgcca
     6601 gcgtcacaaa atgcgcagga cttatgctcg gcaatgcctg cgtagccttg tcgggcgcaa
     6661 tgctttgcca gtatcaaaaa tccgccgata taagccttgg agcgggaatg gtagttgtag
     6721 gacttgcttc ggttataatc ggcgaggctt tggtcggcaa gagaaatgtc attctcggaa
     6781 ttctctcggt ggtagtcggc tcagtcgcgt accaaataat aatagcgatt gcgcttaaag
     6841 tcagactgct gcccgcatac ggactcaaac ttatttcagc ctgcatagtc gcgatttctc
     6901 tgacgctccc cgtagtaagg gacgaaatca aaaaggttag gaggggcaaa aagaatgtct
     6961 gatacgattt taaagctgtg caatgtcaca aaagtgttcc atcctaacac cgtaaacgag
     7021 aatagggcga tagacaacct cagctttgag cttaaaaagg gggatttcgt aaccatttta
     7081 ggctctaacg gctcgggtaa gtctaccatg tttaatctga tagcaggctc actccttgcc
     7141 gacagcggcg aaatctatct tgaaggcgag aatatcaccc ttgttcccga ttatataaga
     7201 gcaagaaaga taggcagaat ctttcaggac cccatgaagg gcacagcccc ttctttgacg
     7261 atagaagaaa accttgctct ggcttactca cggaagtcaa agagcaagat attctccata
     7321 aataaagaag acagaaaggt ctttgttgag ctgctttcaa tgctgggttt gggacttgaa
     7381 aacaggctca agaccaaaat cggacttttg tcgggcggtc aaaggcaagc ggtcaccctg
     7441 ctaatgagca caatcgcaga tccctctctg cttctgctcg atgagcacac cgcagctctt
     7501 gacccgaata ccgctgtaaa aatccttgaa ataacaaaag agcttgtgga atactccaaa
     7561 acttccacaa tgatgataac ccacgacatc aacgccgcac ttagcatagg caacagaacc
     7621 ataatgatgg attcgggcag aataatcctt gacatcagcg gtgaagaaag aaagaacatg
     7681 accgtggagt ctctcttaaa gctctacaaa aaagagagca aaaaggagct ttccaacgac
     7741 agaatattgt tctccgcagg agatatgggc gaggagtaat tctccttgcc atttttcttt
     7801 catgatagta taattaagga aaattaaagc aaacggaggc taaattatgt cattaccagt
     7861 tgctatacag ctttatacgc tgagagacga aatggaaaag gatgtaaagg gcacccttca
     7921 gaaggttaag gaattcggat acgacggcgt agagttcgca ggtctttacg gatattccgc
     7981 cgctgagatg aaggctcttc tcgaagaaat aggcttaatg cccgtttccg ctcatgtggc
     8041 tctcaaggat atccttgccg atatggaaaa tgtaatcgcc acatataagg aaatcggctg
     8101 tgaatatata gctatccctt acctccccgg cgaccgcaga cccgaggctg gcgactttga
     8161 cagcacaatc gccgatattc gcaaaatagg cgaggagtgc aaaaagcagg gcataatcct
     8221 tctttatcac aaccacgact ttgagttcgc gaagcttgac gacaagtatg ttctcgatgt
     8281 gctttatgat tcaatccccg cagaccttct gcagactgag atagatgtct gctgggtaaa
     8341 tgtcgccggc gtcgatcccg ccgaatatgt tcttaaatac acaggacgct caccggtcgt
     8401 tcaccttaaa gacttcgtca tgaagtcaag gcaaaagccc gcaaagctct atgagcttat
     8461 cggcattgag gatgacgaaa agaccgagga agaggatttc tccttcatgc ctgtgggcta
     8521 cggcgtacag gacattcccg gcatcataaa cgcagcggaa aaggctggcg ccaagtggct
     8581 tgttgttgag caggacagac ccgcaaagaa taacactccg ctcgagtccg caaaactgag
     8641 ccgcgactat ctcaaaactt taggctatta aataaaaatt aaagcgataa tcccaacgga
     8701 ttatcgctct ttttttgttt ataaaacctg ctcctttttg tgcattccgg ttatggagaa
     8761 gatgacccat tccgcaatgt tttccgcgtg gtcgccgatt cgctcgtagt atttcgcaat
     8821 ctgcaaaagg tcaaaggcaa gctcgccctt ttttacatct tcatgtatca gttcaatcag
     8881 gctctttttt acctcaagat acagattatc aacgatatcg tccgtggcga taaccgattc
     8941 ggcaagcacc aaatccttct ttacatacgc ctcaatgctg tcggtcacca tctttattgt
     9001 ggttctcgcc atttcgggga tgtattcgtt aaggtgaaaa tcgtcataat cttcagccat
     9061 gtaaacgcaa agctccgaaa tatccgcggc atggtcgccg attcgctcca tatcggtaat
     9121 cattttaagc gcggttgaaa tctgccttaa atcttttgcg accggctgct gactaaggag
     9181 aagttttagg cacatggact caatatcctt ttccttgctg tcaaccgcgt catcgctgct
     9241 tattacatgc tttgcagcct caatatcttt atttataagg gctttagtag cctgcgctat
     9301 gcctctttca atcattgccc ccatctcaat aaggctctca tgcagctctg caagctgctg
     9361 ctcgaatcgg ctcctcatca tccaaacctc cctgtaatat aatcctcagt gcgcctgtct
     9421 ttgggcattg taaacatctt ttccgtcgcc gagtattcaa taagctcacc cataaggaaa
     9481 aatgccgtgt tgtcgctgat tctgagtgcc tgctgcatgt tatgggtgac tattactatg
     9541 ctgtatttat ctctgagggc gataataagt tcctcaatct ttcccgttga gatggggtca
     9601 agggcgcttg tgggctcatc cattaaaagc acctcggggg aaacggcaag ggctctggca
     9661 atgcaaagcc tttgctgctg accgcctgaa agacccagcg ccgacttttt aaggcggtcc
     9721 ttaacctcgt cccaaatcgc ggccttcttt aaagactcct ctacaatctc gtcaagtata
     9781 tgtttttctt ttattccatg ggtcctcgga ccataagcta tattatcata tatactgatg
     9841 gggaagggat taggcttttg aaagaccatt cccacacgct ttcttaagat atttacatcc
     9901 atcttgtcgt aaatgttttc gccgaagagc tttattgtac cctcaatcct gcagccttca
     9961 acaaggtcat tcatgcggtt aagggtcttt aaaaatgttg atttgccgca gccagagggg
    10021 cctataaaag cggtcacctt tttatgctcc agctcaatat ttatgttttt aagggcatga
    10081 aagctgccat agtaaatatt taaaccttca accgtaaagc agttcatgct ttacctccta
    10141 ttctttttgc caccattgcc gaaacggtgt taatcactac tacaagaatc agcagaacta
    10201 ccgccgtagc gtatgcctct ccagtatgca aaccctcgct tgaaagggcg aacatgtgaa
    10261 cggagagggt tcttgcggac tggagtaaat ctttcggaac ctgagccacc gttcccgcgg
    10321 tatatatcag cgcggctgtt tcgccgacta ttctgccgac agccagaatg attcccgcaa
    10381 atatgcccgg aatcgcggcg gggagaacga ctctgaaaac cgtcctcagc tttcccgcgc
    10441 cgagaccgaa tgagccctcc ctgtatgagt cgggaactgc ctttaatgcc tcctcgcttg
    10501 aacggattat aagcggcaga atcattattg acaaggtaaa agagcctgcc agaaccgaaa
    10561 agccccagcc aagatatgtg acgaaaaaca agagtccgaa aagtccgtag ataatcgacg
    10621 gtatgcccga aagggtttcc gttgtaagcc ttatgagatt gacaacctta tttccccgct
    10681 ttgcgtattc cacaagatag attgccgaga acacacccaa tgggacggaa atcgctatcg
    10741 aaatcgccgt cataattaag gttgaaatta gtgcaggcat taaggataca ttttcgcttg
    10801 tgtattttag agagaacaat gacggcttta agtgcggaac gcctttaaac aaaataaagg
    10861 cgattatgaa aaccagcact gagaatgtaa atataaacgc gccgtaggtc attgcggcga
    10921 gaaatttctc ggtcctcttt tgttttttgc tcatttgtcg gacctccttt tcagagccga
    10981 aaagctaagg ttgataataa ggataaacac gaacaatacc acacccgtag caatcagcgc
    11041 atctctgtga agctccgacg catagcccat ttcaataaca atatttgcgg tcaatgtcct
    11101 gacgccctta aacagagagt gggggattct tgcctgatta cccgcaacca taattaccgc
    11161 catagtttcg cctatggccc tgccaatacc aagcacaacg gctgcgagaa gtcccgattt
    11221 cgcggcgggc agcactacgg agaatacgct tttgtagtgc cccgcgccga gggcgagaga
    11281 gccttcgtaa tactgtgtgg gcacagccct caacgcgctt tccgcaagtc ctattactgt
    11341 cggcagaatc ataattccta atagcaaact tgccgtcagc atgctgtagc ccgttatcgg
    11401 cactaaaacc gccatgccga aaaatccgta aacgactgac ggcacgcccg caagcaggtc
    11461 aacggcgggc tttactttct tatagagttt ttttgggcat acaaaggcaa gaaacaccgc
    11521 cgagagaatt ccaatcggca cgccaataag cactgcgccg gcggtaacat aaaggctgcc
    11581 taaaatcatg ggcagaattc cgtattccgc ggggacatcg gagggagccc attcttttcc
    11641 caataggaag tcaaagagcc cgattttcgc cattgcgggt atgccgttca caatcaaaaa
    11701 taggcagata agagcaacgg caaaaatcga agtcagagcc gctatcagga aaactatgct
    11761 cattatcttt tccttagcct tgttcaatca gttccctcca tcttgtaatc tcgcccttaa
    11821 atacagcctt aacctgctca gagcttaaat tgtttatagg gttatttttg ttcactacaa
    11881 tcgcgattcc gtcaagggca attgtgtgct ggtcgagtac cgctttctcg ctgtctttaa
    11941 ggtgtctgct ggacattcct atatcgcagg tgccgtcaat tacaccctga attcccgatg
    12001 aggagtcgct tagctgaatc tcaataatcg cattaggatt aatctcaatg taagcctcct
    12061 tcagcttttc cataacggga gtaacggagg aggaacctgc cacaacgact ttgccgctcg
    12121 gcattgagga attaaactcg ggagcgttgt caactgcgga aatatagctt tcagccgcca
    12181 ctgcttgtcc ctcttttgac agcataaagt ttataaaatc ctgagttgct ggggaaatct
    12241 ctttctttgt aacgattata aaggggcggg atacggcgta agtgccattc tttacggtct
    12301 cggcagtggg gtagaccccg tctatgctta ttagcttaat ggtcttatta acggagccaa
    12361 gggacagata gcctatcgaa tatctgtcgg aggcaatgcc gctcatcagc acatcggtct
    12421 gctttgcgat aacagcctcc tttgtggttc tgtctttgcg ggtaccgttc tcgcccattt
    12481 cgactatatt aaaaatctct gtaaaggcgg ttcttgtgcc tgagccgtcc tctcttgaaa
    12541 acaccgcgat ttctttgtct gccttgaatt cacccttctc tattttatcc aggatg
//
LOCUS       DAONVQ010000053        12112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4036_length_12112_cov_4.338227, whole genome shotgun sequence.
ACCESSION   DAONVQ010000053 DAONVQ010000000
VERSION     DAONVQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4036_length_12112_cov_4.338227"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLD98_04745"
     CDS             <1..737
                     /locus_tag="PLD98_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05590.1"
                     /translation="ALTESEITNIMATSSSVKDAAKRLFDEAREKNSQEDTTVIVFSV
                     ERELPPPVIPEGMYSAPPIAALKSEAPIIDGDEPVYASDDESKFDIRQFASDNKLLIG
                     AIAVLLVVLVLMVAFILSRRSAQPAETTVETTTEEPTTEEVSGIVSEPALDTTVEETE
                     ETTEEPTTEATTEKQTTTTRRSTTTTTQRETTTSRQSTTTTERASESTTVGSVTDITE
                     APSESSTAAPGTEAPTTAAATANTEE"
     gene            complement(822..1742)
                     /locus_tag="PLD98_04750"
     CDS             complement(822..1742)
                     /locus_tag="PLD98_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05591.1"
                     /translation="MMNAVKRTLALLLVLAMLLGIAACGKDKGETTTAEETTAAESES
                     ADETSEPVNGETAADTSAADESTQATEATTGGSGETKAPASETPVSGKPSTKEEIVKY
                     YETAVNKVKSQAKSVAKVYSNAEHYKNILELGNYSALKSIASNLMGKFMKADDKRYDF
                     GGTAAIVENFPPRKVSNVKIDPSTIAEAKCDDKGTYYEVYLKANSSESNPDVNPPTGG
                     GKVGQFVNVVEEKQITDAAGNMVQFKNLENRYFDAWVKAKIDKATGNITELETYLPSV
                     MYFGEVKVTLINAKDISVGLAYIEKWVVNW"
     gene            complement(1834..3051)
                     /locus_tag="PLD98_04755"
     CDS             complement(1834..3051)
                     /locus_tag="PLD98_04755"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HQA05592.1"
                     /translation="MPKTNQRTSGRPFKVNTLIKTLMISGLLLIVVFVAAKSFGADTF
                     TVLGDAISSFFGSARDWDQPYGLNYEDVIDMKPYGSGLAVLTNTEVIYIDSSSNVKDR
                     RSHNYKAPAMKIEGARLLLFDRQSEDYRIEKDSGIVFDSSAESTIITAEIGKRGRCAI
                     AHRGDYATSVLSVYNSVNELIFKWSCEKEHIVDIALSDNGKRVAVAVLGAKDGEIYSK
                     LFVFDIRYKDPIAVFEFDGVSLFSVEYKGGKRFAAAGDSLLCFVNGDKKEDVKTYSPY
                     ELCGYSPIKKGISALCLSKYGNTSDNEITVFDKKGRAIYDTTVKEKVRHFSASEHYVS
                     VLLPDRAESFNKKGEKVANIITDSDGIKIVQNGSSLYILTREGILKHHVPSGKVYNDV
                     EDSINSVESEDNA"
     gene            complement(3066..3800)
                     /gene="truA"
                     /locus_tag="PLD98_04760"
     CDS             complement(3066..3800)
                     /gene="truA"
                     /locus_tag="PLD98_04760"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQA05593.1"
                     /translation="MRNLLLTLSFDGTEFHGWQTQKNAVTVQEVLGDAIFKACGERVT
                     LHGCGRTDAKVHANMFCANFKTESKLSTDSFVSALNYYLPYSIAVKSCEEVDDSFHAR
                     FSCKSKEYVYLIWNARHRNPFYKDRAYHYTYPIDVDKMNKAAESAVGTHDFTSFCASG
                     SEVEDKVRTIFSAKVERDADFVRFTVSGDGFLYNMVRIMVGSLLDIQNGKIEAKTLGE
                     IIEAKDRLKAGFTAPAHGLYLNRVEY"
     gene            complement(3800..4621)
                     /locus_tag="PLD98_04765"
     CDS             complement(3800..4621)
                     /locus_tag="PLD98_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HQA05594.1"
                     /translation="MVRDITIGQYFPGNSVLHNADPRLKLVLSFVSIVFVFVCNNFYS
                     LALVLAFTVVAVLSSRISLKVILKGLRPMLLLLIFTSALQLFYNKQGTELFVSRFITI
                     TTGGIFTTIFMVVRIICLVLLSSLLTYTTSPTQLTDGIERLLSPLKVFGLKNGVHTLA
                     MMMTIALRFIPTLIDEIDKITNAQKARGANFETGGIVQRAKALLPIFIPLLVNSFRHA
                     FELSLAMSARCYNGGEGRTRMKQLKLAARDYVLMAAEFIFVGGVIALNIIFESVI"
     gene            complement(4615..5481)
                     /locus_tag="PLD98_04770"
     CDS             complement(4615..5481)
                     /locus_tag="PLD98_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HQA05595.1"
                     /translation="MSLLELKNVSYTYGAGTPFEITAIKNINLTIEKGSFVGIIGHTG
                     SGKSTLVQHFNALLKPTEGQVLLNGEDINRSKHSAHEARFKVGLCFQYPEYQLFETTV
                     YKDIAFGPTNMGLSEEEIKARVLEAAEFVGLTDELLNKSPFELSGGEKRRAAIAGIIA
                     MRPEVLVLDEPTAGLDPSGRKKILSMICEYREKTDSTVIIVSHSMEDVAAIADKIIVM
                     NKGQVYKYADVHEIFAHSGELASLGLEVPPVTNIFLKLKEMGLDIRTDIYTKDQAEKE
                     LLRYLQKEGIAW"
     gene            complement(5478..6362)
                     /locus_tag="PLD98_04775"
     CDS             complement(5478..6362)
                     /locus_tag="PLD98_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HQA05596.1"
                     /translation="MRGTDTKENLIPIIEFKNACFSYDSEDMGAELSPAVKDINLTIY
                     RGEFVAVLGHNGSGKSTIAKMTNAILRPTSGKVFVEGMDTSDEELEIKIRQLVGLVFQ
                     NPDNQIVSTIVEEDVAFGPENLGVPPAEIRQRVDEALKTVGMFEYRFHEPHKLSGGQK
                     QRVAIAGILAMKTDCIVLDEPTAMLDPVGRREVIGTIKRLNKELGITVVLVTHFMDEA
                     VLADRIIVMNDGTIVKDGTPAEVFSDVKTIKESGLELPIGANMAERLKKSGINLGKGI
                     LTAEDFATAFSQFVVNNR"
     gene            complement(6446..7321)
                     /locus_tag="PLD98_04780"
     CDS             complement(6446..7321)
                     /locus_tag="PLD98_04780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQA05597.1"
                     /translation="MRIATDWLDYELIDCTEGERLERWGDIILIRPDPQVIWKTPKNA
                     RLWGKADARYIRSNTGGGRWEVYKSLPPFWSISYKDLKFNIKTMGFKHTGIFPEQAVN
                     WDLTRGIIEKAVKQGRTPKVLNLFAYTGAATVSALKAGAAVTHVDASKGMTSWAKENA
                     AASGVADKPVRWLVDDCIKFVQREIRRGNKYDIVIMDPPSYGRGPGGEVWKLEEEVYN
                     LTELIKGVLSSDPLMFMISSYTAGLSPAVMAYILGCVMGTSYGKISSDEIGLKASSTG
                     LNLPCGSTAIWTAED"
     gene            complement(7321..8670)
                     /gene="glmM"
                     /locus_tag="PLD98_04785"
     CDS             complement(7321..8670)
                     /gene="glmM"
                     /locus_tag="PLD98_04785"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQA05598.1"
                     /translation="MGRLFGTDGARGIANRDITCELGMKVGRAVAMVLSDISSKRPRV
                     LIGGDTRRSTQMLIASIASGLCSVGSDVLLVGVVPTPAVAYLVKAYGYDAGIMISASH
                     NPCEYNGIKIFKSDGYKLPDELEEKIEEIILDDIGVPPLKIGGDIGDISISNEAIDNY
                     VDHIASSARCKLNGIKVAIDCANGSASATARNLFHKLNLDCDFYNCSPNGTNINKDCG
                     STHMAYISKVVKDNNYDVGFAFDGDADRLLCVDEDGNIVDGDKLMAIIAKHLKEKNML
                     KNNTVVTTVMSNMGFFEMCRKHGINTVQTKVGDRYVLENMLQSGYNFGGEQSGHIIFH
                     DFASTGDGQLTAVQLLNVMCETGKSLKELATVMEVFPQVLINVEVSTLGKLRLDTDKE
                     INLAVEYARKVLGDSGRVLVRASGTEPLVRVMLEGRDEKLINQLGQEIAEVIKERLI"
     gene            complement(8786..9787)
                     /gene="ruvB"
                     /locus_tag="PLD98_04790"
     CDS             complement(8786..9787)
                     /gene="ruvB"
                     /locus_tag="PLD98_04790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQA05599.1"
                     /translation="MVAPGYGAADEDVESTLRPKTLDEYIGQDKPKENLRIYMEAAKS
                     RGENLDHVLLYGPPGLGKTTLASIIANEMNVQIRITSGPAIEKSGDLAALLTNLNEGD
                     VLFIDEIHRLNRSVEEVLYPAMEDYAIDIIIGKGPSARSIRLDLPKFTLVGATTRAGQ
                     LSAPLRDRFGVILRLELYSPEELARIVMRSAGILGIEIDEEGALEIASRSRGTPRIAN
                     RLLKRTRDFAEVVGSGVITLEDARMALERMEIDELGLDSIDRRMLRSIIEIYGGGPVG
                     LETIAALIGEEAITIEDVYEPYLMQLGFISRTPRGRVVTSAAYNHLGIPTNGQQMLF"
     gene            complement(9807..10421)
                     /gene="ruvA"
                     /locus_tag="PLD98_04795"
     CDS             complement(9807..10421)
                     /gene="ruvA"
                     /locus_tag="PLD98_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQA05600.1"
                     /translation="MFYSLTGEVIDFDDSSIAIECSGVGFKCNTSMNTLKRTGKKGDK
                     ITLYTYLSVREDALELYGFHSLQELEMFKLLISVSGIGPKVAVSVLSEFTPDALSLCI
                     ASGDHKSITRAQGVGAKTAQRICMELKDKVTSQSFSFGGDPQELAAVSCVNDNENVSN
                     AVSALVALGYMQAEASRAVCSLDPNLSTQELIKQALRILAKGVG"
     gene            complement(10446..10955)
                     /gene="ruvC"
                     /locus_tag="PLD98_04800"
     CDS             complement(10446..10955)
                     /gene="ruvC"
                     /locus_tag="PLD98_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQA05601.1"
                     /translation="MRVLGIDPGYAIVGYGVLDYNSSKFKVLDFGAVKTKAGESFPCR
                     LQHIFESLIKVIDLWQPEVMAIEKVFHNTNAKTVMDVSQARGVIILAAQVKGLDIVEY
                     TPLQVKQSVVGYGRADKKQVQEMTRIILKLNEVPKPDDTADALALAICHAHSSSASQL
                     IQRASRMSL"
     gene            complement(11101..>12112)
                     /locus_tag="PLD98_04805"
     CDS             complement(11101..>12112)
                     /locus_tag="PLD98_04805"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQA05602.1"
                     /translation="MLVAYTYSGEPVFARQLKAEGAMALLLKDAINPNLVQTLEGSPV
                     LMHGGPFANIAHGCNSVVATKLALKLGDYAITEAGFGADLGAEKFFDIKCRIAGLKPS
                     CAVIVATVRALKYNGGVPKADLSAENIDALKKGIVNLKTHIENVRKFGVTPIVAINRF
                     GTDTQAELSTLISYCEELGVKAVISEGFTKGGEGAAELAKAVCEIADSADTENFKPIY
                     ELDASIKDKLSRIAAEIYRADGVDYSPAANKAIENIEKLGLGGLPVCVAKTQYSLSDD
                     PKKLGKPEGFRIQVKDVRLSAGAGFVVVLLGDIMTMPGLPKTPAAVNMDITEDGKITG
                     LF"
BASE COUNT         2769 a         3104 c         2962 g         3277 t
ORIGIN      
        1 acgctcttac cgaaagcgaa atcaccaaca taatggcaac atcttcaagc gttaaggacg
       61 cggcaaagag gctatttgac gaggcgaggg aaaagaattc tcaggaggac acaaccgtaa
      121 tcgtgttctc ggtcgaaaga gagctgccgc cccctgtaat ccctgaggga atgtactccg
      181 ctcccccgat cgcggcactg aaatccgagg ctcctattat tgacggcgac gagcctgttt
      241 acgcctccga cgatgagagc aagtttgaca ttaggcagtt tgcttcggac aataagctgc
      301 tgattggagc gattgcagta ctgctggttg ttctggtgct catggttgcc tttatactca
      361 gcaggcgctc cgctcaacct gcggaaacca ctgtggagac caccaccgag gaaccgacaa
      421 ccgaagaggt ttcgggcata gtcagcgagc ccgctcttga caccaccgtc gaagagaccg
      481 aggaaaccac tgaggagcca accacagagg ccactaccga gaagcagaca accacaaccc
      541 gcaggtcaac taccacgaca actcagaggg aaaccaccac aagtcgtcag agcacgacca
      601 caaccgagag ggcaagcgag tccacaacgg tcggctccgt aaccgatatt acggaggccc
      661 cctctgaaag cagcacagcg gcgcccggca cagaggcacc cacaaccgcc gctgcaacag
      721 cgaatacgga agaataaaag aagagccgcg aacgcggctc ttttttttgt gctttgaacg
      781 cccgcaaaaa agcggtatcc caaaggatac cgcttttatt tttaccagtt aactacccac
      841 ttttcgatgt aggcaagacc gacgctgatg tccttagcgt ttattaaggt aacctttact
      901 tcaccgaagt acataacgct ggggagatat gtttcaagct cggtgatatt gcctgttgcc
      961 ttatcaatct tagcctttac ccatgcgtca aaatagcggt tctccagatt cttgaactga
     1021 accatgttgc ctgcggcgtc ggtaatctgc ttttcttcaa caacatttac aaactgacca
     1081 accttgccgc cgcctgtggg aggattgaca tcgggattgg attcactgga atttgccttt
     1141 agataaacct cgtaatatgt acccttgtca tcgcattttg cctctgcgat tgtggagggg
     1201 tcaatcttta cattgcttac ctttctgggc gggaagtttt caacgattgc ggctgtacca
     1261 ccaaaatcat accttttgtc gtcagccttc ataaatttgc ccataaggtt tgatgcaatg
     1321 ctttttaatg cggagtagtt tccaagctca agtatgttct tgtagtgctc ggcgttggaa
     1381 tagacctttg ccacgctctt tgcctgagac tttaccttgt tgacggctgt ttcatagtat
     1441 ttgactatct cctcttttgt ggagggcttg ccgctgacgg gagtctcgga tgcgggagct
     1501 tttgtttcgc cgctgccgcc ggtggtggct tcggttgcct gagtgctttc gtctgccgct
     1561 gatgtgtccg cggcggtttc gccgtttacg ggttcgctgg tctcatctgc ggactcgctt
     1621 tctgccgctg tggtctcttc cgcagttgtg gtttcgccct tatccttgcc gcatgcggca
     1681 attccaagga gcattgcaag tacaagaagt agggcaagcg ttctctttac agcattcatc
     1741 attagttacc tccgtaaatg aagttaagaa gacttgtttt gattttacct agtgtatgag
     1801 tattagtcaa tagcgatgtt gttttttacc agtttacgca ttatcttcgc tttccacact
     1861 attaatacta tcttcgacat cattatatac tttgccggag gggacatggt gctttaaaat
     1921 gccctctctt gtgagtatat acaggctgct gccgttctgc acgattttga tgccgtcgct
     1981 gtcggtaatt atatttgcga ccttttcgcc ctttttgtta aagctctccg ctctgtcggg
     2041 cagaagaacg ctgacatagt gttccgacgc ggaaaaatgc cttacctttt ctttaacggt
     2101 tgtatcataa atagctctgc cttttttgtc aaagacggtg atttcattgt cggaggtgtt
     2161 gccgtacttt gaaaggcaaa gcgcggaaat gcccttcttt atcggggaat agccgcatag
     2221 ttcatagggc gaataggttt tgacatcttc ttttttatcg ccgtttacaa agcaaagaag
     2281 gctgtcgccc gccgcggcaa aacgcttgcc gcctttatac tcaacggaga aaagggacac
     2341 gccgtcaaac tcaaaaactg ctatcgggtc tttgtatctt atatcaaata caaagagctt
     2401 tgagtagatt tctccgtcct tggcgcccaa aacagcgacc gcgacccgct tgccgttgtc
     2461 ggaaagggct atatccacaa tatgctcttt ttcgcagctc catttaaaga taagctcatt
     2521 taccgagttg tatacggaca aaacgctggt ggcatagtcg cctctgtggg ctatggcgca
     2581 gcggcctctt ttgcctatct cagcggttat gatggtggac tcggcggagg aatcaaatac
     2641 tattccgctg tccttttcta ttctgtaatc ctcggactgc ctgtcaaaaa gcagaagcct
     2701 tgcgccctca attttcatgg cgggagcttt gtagttgtgg ctgcgcctgt cttttacatt
     2761 tgaggagctg tctatgtaaa taacctcggt gtttgtaagt accgcaagcc ctgagccgta
     2821 gggcttcata tctataacat cttcgtaatt taaaccgtag ggctgatccc agtctctcgc
     2881 ggaaccgaaa aaggaggaaa tagcgtctcc caaaacggtg aaggtatcgg cgccaaagct
     2941 ctttgcggcg acaaaaacaa caatcagcaa aagccccgaa atcatcaggg tttttataag
     3001 agtattgacc ttaaacggtc tgccgcttgt cctttgattc gtctttggca ctcggattcc
     3061 tcccgtcagt attctaccct gttaagatac agcccgtggg cgggggcggt aaagcccgct
     3121 ttgagtctgt cttttgcctc tataatttct cctaaggttt ttgcctctat ttttccgttt
     3181 tgtatatcaa gaagtgagcc gaccataatt ctgaccatgt tgtataaaaa gccgtcgccc
     3241 gaaaccgtaa agcggacaaa gtcagcgtcc ctttcaacct ttgcggagaa tatggttctt
     3301 accttgtctt cgacctcgct gcccgatgcg cagaaggagg taaagtcatg ggttccgacg
     3361 gcggattctg ccgccttgtt catcttatca acatctatgg gataggtgta gtgatatgcc
     3421 ctgtccttat aaaaggggtt gcggtgacgg gcgttccaga taaggtacac atactccttg
     3481 gacttgcagc taaatcttgc gtgaaagctg tcgtccactt cctcgcagct ttttaccgct
     3541 atggaataag gcagataata gtttaaagcc gacacgaagg agtctgtgga gagcttgctt
     3601 tcggttttaa agtttgcgca gaacatattg gcgtggacct ttgcgtctgt tctgccgcag
     3661 ccgtgcaagg ttactctctc gccgcatgcc ttgaaaatgg cgtctcccag tacctcctga
     3721 acggtcacgg cgtttttctg cgtctgccag ccgtggaact ccgtaccgtc aaaggagagg
     3781 gtgagaagta aattgcgcat tatatcaccg actcgaaaat tatgttaagg gctatgactc
     3841 cgccgacaaa gataaattcc gccgccataa ggacataatc ccttgcggca agctttaact
     3901 gtttcattct cgttcttccc tctccgccgt tgtagcagcg ggcggacatg gcaagggaca
     3961 gctcaaaggc gtgtctgaaa gagtttacaa ggaggggtat gaaaatgggc aggagcgcct
     4021 tagccctttg gactatgccg ccagtttcaa aattcgcgcc ccttgctttc tgggcgtttg
     4081 taatcttatc tatttcgtca atgagagtcg ggatgaatct gagagcaatt gtcatcatca
     4141 tggcaagggt gtgcaccccg tttttaagcc cgaaaacttt taggggagat aacagccttt
     4201 caatgccgtc agtgagctga gttggtgagg tggtataggt taatagcgaa cttaaaagca
     4261 caaggcagat gattcttaca accataaaaa tggtggtgaa aattccgccc gtggtgatgg
     4321 tgataaagcg gctgacaaac agctctgtgc cctgcttatt gtaaaaaagc tgaagcgccg
     4381 aggtaaatat aagcagcaaa agcatgggtc tgaggccctt gagaatgacc tttaagctga
     4441 tgcgggagga aagtaccgcc acaactgtaa aggctaaaac aagggcaagg gaatagaaat
     4501 tattgcatac gaatacaaag actatggaaa caaaggagag gacaagctta agtctggggt
     4561 cagcgttgtg gaggacggaa ttgcccggaa aatactgacc tatggtaatg tctcttacca
     4621 agctattccc tccttttgca agtatcttaa aagctccttt tcggcctggt ccttggtgta
     4681 gatatcggtt ctgatatcaa gtcccatttc cttcagtttc aggaatatgt tcgtaacggg
     4741 aggcacctca agccccaggg aggccagctc gccgctgtgg gcgaaaatct cgtgaacatc
     4801 ggcgtatttg tagacttgac ccttgttcat gacaatgatt ttgtctgcga tggcggcaac
     4861 atcctccata ctgtgggaga cgatgatgac cgtgctgtcg gttttctccc tatactcgca
     4921 aatcatgctg agaatctttt ttctgccgct ggggtcaaga cccgcggtgg gctcgtcaag
     4981 aaccagcact tcggggcgca tggctataat gcccgcgatg gccgcccttc tcttttcgcc
     5041 gcccgacagc tcaaaggggg atttattcaa aagctcgtcc gtgagaccta caaactcggc
     5101 ggcctcaaga actcttgctt ttatttcttc ttctgaaagc cccatgttcg tgggtccgaa
     5161 agctatgtcc ttatagacgg tggtttcaaa aagctgatat tcgggatact gaaagcacag
     5221 tcccacctta aagcgtgcct cgtgggcgga atgcttgctt ctgtttatgt cctcgccgtt
     5281 taaaagcacc tgaccctcgg taggctttaa tagggcgttg aagtgctgca caagagtgct
     5341 tttgccagag cctgtatgac cgattatccc cacgaaggag cctttctcaa tcgtgaggtt
     5401 gatgttcttt atcgccgtta tctcaaaggg cgttcccgcc ccgtaggtat atgacacatt
     5461 tttaagttca agcaagctca tctgttgttc accacaaact gactgaaggc agtcgcgaaa
     5521 tcttccgcag tcagtatacc tttaccaaga tttattccgc tctttttaag tctttccgcc
     5581 atattggcgc cgatgggcag ctcaagtccc gattctttaa tggtctttac atcgctgaaa
     5641 acctcggcgg gggtaccgtc cttgactatg gtaccatcgt tcatgactat gattctgtcc
     5701 gcaagaaccg cctcgtccat aaagtgggtg acaagcacaa cggtaatgcc aagctctttg
     5761 ttgagcctct ttatggtgcc gattacttcc cttctgccaa cggggtcaag catagctgtg
     5821 ggctcgtcaa ggacaatgca gtcggttttc atggcgagta tgcccgcaat cgccacacgc
     5881 tgcttttgtc ctcccgacag cttatgaggc tcgtgaaatc tgtactcgaa cattccgacg
     5941 gtttttaaag cctcgtccac cctttgcctt atctcggcgg gaggcacgcc cagattctcg
     6001 ggtccgaacg ccacatcctc ctcgacaatg gttgagacta tctgattgtc ggggttttgg
     6061 aaaacaagac ctacaagctg ccttatcttt atttcaagct cctcgtcgga ggtgtccatg
     6121 ccttcaacaa aaaccttgcc gcttgtgggg cgcagtatcg cgtttgtcat cttcgctatg
     6181 gtgcttttgc cagagccgtt gtgccccaaa acagcaacaa attccccgcg gtaaatggta
     6241 aggttaatat cctttaccgc aggcgaaagc tccgctccca tatcttccga atcgtatgaa
     6301 aagcaggcgt tcttaaattc aattatgggg ataaggtttt ctttagtgtc cgttccgcgc
     6361 atttttatgt cctttccgag tttgtatcac gaaagaacat atcgaccgta agtaagctgc
     6421 taacaaacat tcagtctatg tccttttaat cttccgctgt ccaaatcgcg gttgagccgc
     6481 aagggagatt gagccccgtt gaggatgcct tgagacctat ctcatcggag ctgattttgc
     6541 cgtaggaggt gcccatgacg cagcccaaaa tataagccat aaccgccggg gaaagccctg
     6601 cggtgtagga gctgatcata aacataagcg ggtcgctgct tagcacgcct tttattagct
     6661 ctgtgaggtt atagacttcc tcctccagct tccagacctc gcccccgggg cctctgccgt
     6721 aggatggcgg gtccataatt actatatcgt atttattgcc gcgccttatc tctctttgaa
     6781 caaacttaat gcagtcgtcc accagccatc ttacaggctt gtccgctacc ccggaggctg
     6841 cggcgttttc ctttgcccag gaggtcatgc cctttgaggc gtcaacatgg gtaactgcgg
     6901 cgcccgcttt caatgctgaa acggttgccg ctcccgtata ggcaaagaga tttaagacct
     6961 tgggggttct gccctgcttt acggcttttt cgataatgcc ccttgtaagg tcccagttga
     7021 ccgcctgctc ggggaatatg cccgtatgct taaagcccat tgttttaata ttgaacttta
     7081 agtctttata tgaaatactc caaaaaggcg gtaggctttt atatacttcc cacctgccgc
     7141 cgcctgtgtt tgagcgtata tatcttgcgt cagccttgcc ccaaaggcgg gcatttttag
     7201 gcgttttcca gataacctga gggtcggggc ggataagtat aatatccccc cacctttcaa
     7261 gcctttcgcc ctcggtgcag tctataagct cataatccaa ccagtcagtc gctatacgca
     7321 tcagattagc ctttctttaa ttacttctgc aatttcctgt cccagctgat taatcagctt
     7381 ttcatccctg ccctcaagca tgactctgac aagaggctcg gtgccggagg ctctgacaag
     7441 cactctgccc gaatcgccca ggactttcct tgcgtactcg acggcaaggt ttatctcctt
     7501 gtcagtgtca agacgcagct tgccgagggt ggaaacctcc acattgatga gaacctgcgg
     7561 gaatacctcc ataacggtgg ccagctcttt aaggctcttg cccgtttcgc acattacatt
     7621 aaggagctga acggcggtga gctgtccgtc tcccgttgag gcgaagtcat ggaaaattat
     7681 gtgacccgac tgttctccgc caaaattata accgctttgg agcatatttt caagaacata
     7741 tctatcgccg accttggtct gaacggtgtt tatgccgtgc ttgcggcaca tctcgaaaaa
     7801 gcccatgttg ctcattacgg tggtgacgac ggtgttgttc ttgagcatgt ttttctcttt
     7861 taagtgctta gcgattatcg ccatcagctt gtcgccgtca acgatattgc cgtcctcgtc
     7921 aacgcacaaa agcctgtcgg cgtcgccgtc gaacgcgaag cccacatcgt agttgttgtc
     7981 ctttacaacc tttgagatat acgccatgtg ggtggagccg caatccttgt tgatattggt
     8041 gccgttgggg ctgcagttgt aaaagtcgca gtcgaggttg agtttgtgga acaaattcct
     8101 tgccgttgcg gaggctgagc cgttggcgca gtcgatggcg actttgatgc cgttcagttt
     8161 gcatcttgcg gatgaggcga tgtggtccac atagttgtcg attgcctcat ttgagatgct
     8221 tatgtcgcct atatctccgc cgattttaag gggcggaact ccaatgtcgt caaggatgat
     8281 ttcttcgatt ttctcctcaa gctcatcggg gagcttgtag ccgtcggatt tgaatatctt
     8341 gattccgttg tattcacagg ggttgtggga cgcggaaatc ataatgcccg cgtcgtagcc
     8401 gtaagccttt accagatacg cgacggcggg agtcggcaca acgccgacaa ggagcacatc
     8461 gctgcccacg gagcaaagtc ccgacgctat tgaagctata agcatctgcg tggaccgtct
     8521 tgtgtctccc ccgattaaaa cccttggtct ttttgagctg atgtcggaca ggaccatcgc
     8581 taccgccctg ccgaccttca ttccaagctc gcatgtaata tcgcgattgg ctatgcctct
     8641 cgcgccgtct gttccgaata gtctgcccat aattcctcct ccagtgcggc aaataagcgc
     8701 tttaccgcaa aagataaaag tttaatttgg cataaaagta tatgccaaaa ttctcaaata
     8761 tattacaaaa tctatttttt tacgactaaa aaagcatttg ctgaccgttt gtgggaatgc
     8821 cgagatgatt gtacgcggcg ctggtgacta ccctgcccct gggtgttcgg cttataaaac
     8881 caagctgcat aagatagggc tcgtagacat cctctattgt gattgcctcc tcgccgatta
     8941 aagcggcgat ggtttcaagt cccacgggac cgccgccgta gatttcgata atagacctta
     9001 gcatccttct gtctatgctg tcgagtccca gctcgtcaat ctccattctt tcaagcgcca
     9061 ttctggcgtc ctcaagggtg atgacgccgc tgccgacgac ctcggcaaag tcccttgtac
     9121 gctttaaaag gcggttggcg attctgggcg tacctcttga acgggaggcg atttcaaggg
     9181 ctccctcctc gtcgatttca ataccaagta tgcccgcgct gcgcattact attctggcca
     9241 gctcctcggg ggaataaagt tcaaggcgca gtatgacgcc gaacctgtcc cttaacggag
     9301 cagaaagctg acccgccctt gtggtggctc ccacgagggt gaatttgggc aaatcgagcc
     9361 ttatcgacct tgccgacggg cctttgccta tgattatgtc aatggcgtag tcctccatgg
     9421 cgggatagag aacttcctca acgcttctgt tgagcctgtg gatttcgtct ataaacagga
     9481 catcgccctc gttgaggttt gtaagcagag ccgcaaggtc ccccgacttt tcgattgcgg
     9541 gacccgatgt gattcttatc tgaacattca tctcattggc gataatgctg gcgagagtgg
     9601 ttttgcccag tcccggaggt ccgtagagca gcacatggtc aagattctct cctctgctct
     9661 ttgcggcctc catatagatt ctcaggttct ccttgggctt gtcctgaccg atatattcat
     9721 cgagagtctt tggtctaagg gtggattcca catcctcgtc cgcggcgccg tagccgggag
     9781 ctaccattct taaatcctca gccaaatcat cctaccccct tagccaaaat tcttagtgcc
     9841 tgcttaataa gctcctgagt gctcaaattc gggtcaaggg agcagacagc ccttgacgct
     9901 tccgcctgca tatagcccag agccaccagt gcgctgaccg cgttggagac attttcgttg
     9961 tcattaacgc aggatacagc cgcaagctcc tgcgggtcgc cgccgaaaga gaaactttgg
    10021 gaggtgacct tgtccttcag ctccatgcag attctttggg cggttttcgc tcccacgccc
    10081 tgagctcttg tgatggattt atggtcgccc gatgcgatgc agagggaaag ggcgtcggga
    10141 gtaaactccg agagaaccga aacggcgacc ttgggaccta tgcccgaaac ggagataagg
    10201 agcttgaaca tctcgagctc ctgaagtgag tgaaagccgt aaagctccaa agcgtcctct
    10261 cttacactca gatatgtata aagggtgatt ttgtccccct tttttcctgt gcgttttaaa
    10321 gtattcatgc tggtgttgca cttaaagccc acgcccgagc attctatggc gattgagctg
    10381 tcgtcaaagt caattacttc acctgtcagg gaatagaaca ttttacactt cctttccgaa
    10441 aggaattaaa gactcattct gcttgccctt tgaatcagct gcgaggcgga gctggaatgg
    10501 gcgtggcaga ttgcgagggc aagggcgtcc gctgtatcgt cgggctttgg gacctcgttt
    10561 agctttagga ttatccttgt catttcctga acctgctttt tgtccgccct gccgtagccc
    10621 acaacgcttt gctttacctg aagcggcgtg tattccacaa tgtcaaggcc ctttacctga
    10681 gcggcaagta taattactcc ccttgcctgg ctgacatcca taaccgtctt ggcgtttgtg
    10741 ttgtgaaaga ccttttctat cgccataacc tcgggctgcc acaagtctat aacctttatc
    10801 aggctctcaa aaatgtgctg aaggcggcag ggaaaagact ctcccgcctt tgtttttaca
    10861 gctccgaagt caagcacctt aaattttgat gaattgtaat ccagtacacc gtagcccact
    10921 atggcgtagc ccgggtcaat gccaagcacc ctcaagcaaa gcacctctta tgtatctgtt
    10981 aatttatgta ttataccaca cactgtatca aaactcaatt attcgccgaa acggcgcggg
    11041 cgcaaaacga caaaggaaaa aggagcagcg ggttctgctc cttctattta gaaaatctat
    11101 ttagaaaaga cctgtgattt ttccgtcctc tgtaatgtcc atattcaccg cggcgggggt
    11161 cttgggcagt cccggcattg tcataatgtc gccgagaagc accaccacaa agccggcgcc
    11221 cgcggagagt cttacatcct tgacctgaat tctgaagcct tcgggcttgc ccagtttctt
    11281 cgggtcgtcg gaaagtgaat actgggtttt agcgacgcag acggggagac cgccgagtcc
    11341 cagcttttca atattctcaa tagccttgtt cgcggcgggg ctgtagtcca cgccgtcggc
    11401 tctgtaaatt tcggctgcta ttcttgagag cttgtccttt attgaagcgt caagctcata
    11461 gatgggctta aagttttctg tatccgcgct gtcggcaatt tcgcaaacgg ctttagccag
    11521 ctccgcggca ccctcgccgc cctttgtaaa gccctcagaa ataaccgcct ttacgccaag
    11581 ctcttcgcag taggaaataa gcgtgctcag ctctgcctga gtgtctgtgc cgaagcggtt
    11641 aattgccaca atgggggtta cgccgaattt gcgcacattt tcaatatgag tcttaaggtt
    11701 cacaatgccc ttctttaaag cgtcgatgtt ttcggcgcta aggtctgctt tcggcactcc
    11761 gccgttgtat ttaagcgctc tgacggttgc cacgataacg gcgcaggagg gtttgagacc
    11821 tgctattctg cacttaatgt caaagaattt ctccgcgccc aaatcagcgc cgaagcccgc
    11881 ctcggtaatt gcgtagtcac cgagcttgag agcgagctta gtagccacaa cggagttgca
    11941 gccgtgggct atattggcga agggtccgcc gtgcatgagc acgggtgagc cttcaagagt
    12001 ttgaacaagg tttggattaa tggcgtcctt taaaagcaga gccattgcgc cctccgcttt
    12061 aagctgacgg gcaaataccg gctcgcccga ataagtgtag gcgacaagca tg
//
LOCUS       DAONVQ010000054        12067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4054_length_12067_cov_4.958625, whole genome shotgun sequence.
ACCESSION   DAONVQ010000054 DAONVQ010000000
VERSION     DAONVQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12067
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4054_length_12067_cov_4.958625"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLD98_04810"
     CDS             complement(<1..432)
                     /locus_tag="PLD98_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HQA05603.1"
                     /translation="MPLNKDIKKVLVIGSGPIVIGQAAEFDYAGAQACTVLKQAGLDV
                     VLVNSNPATIMTDKTLADEIYLEPLNEETIKRIIIKEKPDSLLASLGGQTGLTIAMQL
                     SKEGFLEKHGVKLLGTNAASIDKAEDREQFKNTMLEIGQPII"
     gene            complement(419..1504)
                     /locus_tag="PLD98_04815"
     CDS             complement(419..1504)
                     /locus_tag="PLD98_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HQA05604.1"
                     /translation="MKKAYIALSNGKVFEGYRFGAEKDAVGEIVFTTGMCGYIETMTD
                     PSYYGQIVLQTFPQIGNYGFISEDAESPKCYMKGYIVREWSEYPSNFRSEGDIDSYLK
                     KEGVPGVYGVDTRELTRIIREYGVMNAMITDDLSKVDYEKLNEYRVKDAVKGVSSGKT
                     SVLTPEADILYSVALIDYGAKSNIARELLKRGCKVHIVPSTTTAEEIMELNPDGIMLS
                     NGPGDPAENIECIEVLKKLIGKKPMFGICLGHQLLALAHGGRTEKLKYGHRGANQPVK
                     DLQSGRVFITSQNHGYVVVNDSIADKGGIVRLINANDGTCEGIDYPELKAFSAQFHPE
                     ACAGPQDTSFMFDRFVNLMRSEKDAIK"
     gene            2307..3434
                     /locus_tag="PLD98_04820"
     CDS             2307..3434
                     /locus_tag="PLD98_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HQA05605.1"
                     /translation="MEFLYSGIMSITWQMVVMYIIGGLLIYLALEKDFEPALLLPMGF
                     GAILVNLPFSGVINQTMEGIGETHGIVQWLFDVGIEASEAMPLLLFIGIGAMIDFGPL
                     LSNPKMFLFGAAAQFGIFAAITISTLLGFDIKDAVAAGMIGAADGPTAILVSQVLQSK
                     YVGAIAVAAYSYMALVPTIQPAVIKAITTKKERLIRMPYNPKTVSKRTRILFPIIVTV
                     IAGLVAPASVSLVGFLMFGNLIRECGALDNLSKTAQNELANLVTLLLGITISFSMVAE
                     KFVTFDTLKIMVIGLLAFVFDTIGGVMFAKFLNLFSKNKINPMIGAAGISAFPMSARV
                     VQKMALAEDKQNHLLMHAIGANVSGQIASVVAGGVLLGFFK"
     gene            3447..3629
                     /locus_tag="PLD98_04825"
     CDS             3447..3629
                     /locus_tag="PLD98_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05606.1"
                     /translation="MLLNLINLAFEINKETVLTSLDFMWKGMLGIMTVIVVITLIVTL
                     MAKLDSKAEKKSGSQS"
     gene            3769..5202
                     /gene="purB"
                     /locus_tag="PLD98_04830"
     CDS             3769..5202
                     /gene="purB"
                     /locus_tag="PLD98_04830"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQA05607.1"
                     /translation="MSSAYVSPLSKRYASKEMQEIFSDDSKFTTWRRLWVALAEAEQQ
                     LGINITDEQIEELRANINNIDYEMARQKELEVRHDVMAHVLTYGEACPKAKGIIHLGA
                     TSCYVGDNTDIILMKKALLRVKKLLLNAIAALSDFALKYKDQPTLAFTHFQPAQPTTI
                     GKRATLWLHDLLLDFEQLEFTLKNLKLLGCKGTTGTGASFLELFDGDHEKVKKLEMII
                     AEKMGFDAVYPVSGQTYSRKVDYQVLSVLSSIAQSACKFAGDIRLLSHLKEVDEPFEQ
                     HQIGSSAMAYKRNPMRSERIASLSRFVIATAENPAYTAATQWFERTLDDSANKRLSVP
                     EAFLAIDGILSLYINIVKGMRVYTKMAEKHLNEELPFMATENILMYCVKNKGRDRQEL
                     HEAIREHSVAAAYNIKEYGGDNDLLQRILSDSRFGLTKEELDNILDVRRFTGRAKEQT
                     EEFIEEYIKPILEKNKEFLGIDIDIRV"
     gene            5232..6506
                     /locus_tag="PLD98_04835"
     CDS             5232..6506
                     /locus_tag="PLD98_04835"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQA05608.1"
                     /translation="MLTAVVGINWGDEGKGRMVDLFAADYDIVIRYQGGNNAGHTVIN
                     EKGKFILNLLPSGILSETTVNVMGNGMVIDLEHLVKEIASLTEKGIKITPENLKISDK
                     AVICMPYHKLLDCLEEDRLGDAKFGSTRRGIAPVYSDKYLKKALRMGDLFYFDELEKK
                     LGPLMEWKNLTVRDGYKHEEIKPEDMLEWLKKYGEPLLPHICDTTVYLNKAAEEGKNI
                     MFEAQLGALRDIDFGIYPYTSSSSTIAAYAPIGAGIPNRKIDEVIGIMKAYSSCVGEG
                     PFTAELFGKEGDELREAGGEYGAATGRPRRVGGFDVVASRYGAMVQGATSIALTKLDV
                     LSYLDKIPVCVAYEIDGEIVHDFPTGARLNRAKPVYEYFDGFKEDISHCRKLEDLPEN
                     ALKYVKYIEEQVGCKISYISVGAKRDQYIKVD"
     gene            6542..8068
                     /gene="guaA"
                     /locus_tag="PLD98_04840"
     CDS             6542..8068
                     /gene="guaA"
                     /locus_tag="PLD98_04840"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQA05609.1"
                     /translation="MKNQLVLVLDFGGQYKELIARRIRELNVLSMVLPGDTPVERIKE
                     LSPIGIIMTGGPDSVNAPDAYLCSKEVFELGIPVLGICYGCQLIAHLLGGKVGTCDVS
                     EYGTVKAKLSPESVLFENIKPESKVLMSHTNLVTQLPEGFVSTASTRSCKIAAIENKE
                     RKIFGLQFHPEVAHTENGTEIIKNFLYNVCGAKGDYDMAGYIETQIQEIREQTKGKKV
                     LLALSGGVDSSVCAALLSEAIPGKLVCIFVDHGFMRKNEADEVEAVFSKRNLQFVRVD
                     ASERFLSKVKGVTDPERKRKIIGEEFIRVFEEQAAKFGDVSFLAQGTIYPDIIESGHG
                     KAAVIKSHHNVGGLPEDIRFEGIVEPLKGLFKDEVRKVGRELGLPESIVERQPFPGPG
                     LAVRVIGEITKEKLDILREADAIFREEVGKLKKKPSQYFAVLTNLRSVGVMGDERTYD
                     YTLALRAVNTGDFMTATYSKIPHSVLNKISRRITNEVKGINRVVFDITDKPPATIEWE
                     "
     gene            complement(8164..9957)
                     /locus_tag="PLD98_04845"
     CDS             complement(8164..9957)
                     /locus_tag="PLD98_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05610.1"
                     /translation="MMQLAPQGNPVWDLAGRLCDTYLKFKERLQPYNIPTGILVQSVL
                     GHGHPLVHAPFQRYVNLDDGASPNVYCPYDDDFCEYMKDSMAKIASCNPDVIMIDDDM
                     RMMMWRSGKGGGVNACCCPLHKAEFTRRTGVEMSHEELYEHVLTHEKDDPYTKVLREI
                     QHDSLIKIVRAMREGIDSVDPSIQGANCATGYVCESMKDMAHIFAGKGNSPIVRVPNG
                     TYAPSSNRNFSLTMFRLSVCKEKLREDGIDTIIAETDTVPYNRYAKSARYLHAHMAVS
                     VFDGAKGAKHWISRFGASEPNSGKAYREILAKHAKFYDRLSELSADLTPVGCCVPFVT
                     QYELDFRPNKNNLNCAWATKVFERMGLPFYFSNKFEGAVFLSENLIPQMSDEQIERMF
                     EDSSVFLDSVSAELLIERGFERFLGVKAVSYEGKTIFGEIFEDSGNMCARQYEMKELK
                     VTADGVRVLSHCYHQTQTGIERVCPAVTVLPRKNGRLTVVYCGTPDTPWNYIDAFSFL
                     NESRKRQFVSLLKEANSLPVYYVGDNEICLRAGVTPDGRLVAMFINLGTDPEETLTIG
                     LEKEPKDIRILLPDGSTAKCDWKPSGEGIKL"
     gene            10288..10575
                     /locus_tag="PLD98_04850"
     CDS             10288..10575
                     /locus_tag="PLD98_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05611.1"
                     /translation="MANVDVNVTAQALEANTPYEVVDITFIYSDATEQNGNVIYYGAT
                     LTDGTVSEDVLFSYAVTPGTDIPASVSFAYEPTVAFTDTITGSNGKVYYTP"
     gene            10804..11247
                     /locus_tag="PLD98_04855"
     CDS             10804..11247
                     /locus_tag="PLD98_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05612.1"
                     /translation="MEPRQIDCAKLILYKAPPFGRRDENAQCEENRYYLFPLLDYFSV
                     YSCKFCKPAIDTCRRVVFYNDYCKSYTEIDITSIVKDWLDFNIENKGLLLVGSGHSEC
                     VFYESNRSDFVGMRPTLRLFCSGISPFPTPTLACADCDVVINRPS"
     gene            11658..>12067
                     /locus_tag="PLD98_04860"
     CDS             11658..>12067
                     /locus_tag="PLD98_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQA05613.1"
                     /translation="MSKKYRFETLQVHAGQEKPDPATDARAVPIYATTSYVFPGAESA
                     AARFGLREVGNIYSRLTNPTNSVFEERINALEGGIGALATASGSAAITYAILNIATAG
                     SHIVSDHRIYGGTYNLFANTLKDYGIETTFVSGD"
BASE COUNT         3221 a         2905 c         2807 g         3134 t
ORIGIN      
        1 aattatgggc tgacctattt ccagcattgt gttcttaaac tgctctctgt cctccgcctt
       61 gtcaatggag gcggcgtttg tgccgaggag ctttacgccg tgcttttcaa gaaagccctc
      121 ctttgaaagc tgcatggcga tggtaagtcc cgtctgaccg ccaaggcttg ccagcaggct
      181 gtcgggcttt tcttttataa taatcctttt aattgtttcc tcatttaacg gttcaagata
      241 aatttcatcg gcaagagttt tgtccgtcat gatagttgcg gggttggagt taacaagcac
      301 gacatcgagc cccgcctgct tgagcaccgt gcaggcctga gcgcccgcat agtcaaactc
      361 cgcggcctga ccgattacta tcggacctga gcctataacc agtacctttt ttatatcttt
      421 atttaatggc atctttctca ctcctcataa ggttgacaaa gcggtcgaac ataaagctcg
      481 tatcctgcgg accggcgcag gcctcggggt ggaactgcgc ggagaatgcc tttagctcag
      541 gatagtcaat gccttcgcat gttccgtcgt tcgcgtttat aagccgtact ataccgcctt
      601 tatcggcaat gctgtcgttt acaactacat agccgtgatt ctgactggtg atgaatactc
      661 tgccgctttg caggtcttta acgggctgat ttgcgcccct gtggccgtat ttgagctttt
      721 cggttctgcc gccgtgggcg agagcgagga gctgatgacc gaggcagatt ccgaacatgg
      781 gctttttgcc aattagcttc tttaaaacct ctatgcactc tatgttctca gcggggtctc
      841 cggggccgtt tgagagcatg attccgtcgg ggttcagctc cataatctcc tcggcggttg
      901 tggttgaggg aactatgtgc accttgcatc ctctctttaa tagctccctt gctatattcg
      961 actttgcgcc gtagtcaatc agcgcaactg aatataaaat atcggcttcg ggtgtcagca
     1021 cagaagtttt gccgctgctt acacctttaa ccgcatcttt tactctgtat tcatttaatt
     1081 tctcgtaatc aactttgctg aggtcgtccg taatcatggc gttcataacg ccgtattccc
     1141 tgattatcct tgtaagctcc cttgtgtcaa cgccgtaaac accgggaacg ccttccttct
     1201 ttaaatagga atcaatatcc ccttcgctgc ggaaatttga aggatattcg ctccattcgc
     1261 gaacaatgta gcccttcata tagcactttg ggctttccgc gtcctcagaa ataaagccgt
     1321 agtttcctat ttgaggaaag gtctgaagaa ctatttgacc gtagtagctg gggtcggtca
     1381 ttgtttcaat gtagccgcac attccggttg tgaaaacaat ttcgccaacg gcatcctttt
     1441 cggcaccaaa gcggtagccc tcaaacacct tgccgttgct cagcgcaata taggcttttt
     1501 tcatagcatc aaccagtcct tttctattgc atttaagttt tgcgtctttt cagtgtaaca
     1561 aacgctcttt gttgccagac tgagtatctg ccaaatcaga cttagcacat actgacgctc
     1621 cgtttgaaaa atcatgccgt aaaatccgcc gcattttaaa aaaacgcgct gacgggaata
     1681 tgagtcgtca gcaggcggga ttatgatttt tcacggcttt gccgcttatc ttgcggcttt
     1741 ctatgtccac ttaattatag agacactgga aaggtatgtc aatagctccc gaacgcattt
     1801 tttgagtttg taaagagtgt ataaatataa gccgaattta gtatatattt atggtgaatt
     1861 gtatatttat tctttgagca atcttgccga aattttattt atctaacccg gtaaaaaggg
     1921 gaaccaataa taaccacccc ttcagagcgc ttttcaaatc aagattttcc acatgagaat
     1981 ataaaatgaa tcagttttag atgccataaa aagggataaa tttttgaaga aaagctgaaa
     2041 accaaaattg tattattata cttccaaaaa atacttgaaa tcttgtccgt cttagtctat
     2101 actctactta taatggggtt ggatggtgtt tttttgggaa aacacttccg agccaagcaa
     2161 aacagataat gcggatgtcc ttttaagtcc ttttaggtta atctgtgttt tttgttttca
     2221 gattaaattc tcaggctgtc gcaagcggtg ttttgctcat gcccacttat gctgcggcag
     2281 gctaaaattt ttttggggtg atttgtttgg aattcctata ttcgggaata atgagcatta
     2341 cttggcaaat ggtggtaatg tacataatcg gaggtctgct catatacctt gctttggaaa
     2401 aggattttga gcccgccctc cttctcccta tgggctttgg cgcgattctt gtaaacctgc
     2461 ccttctcggg agttatcaat cagacaatgg aaggcatagg cgaaacccac ggcatagtcc
     2521 agtggctttt tgatgtggga atagaagcct ccgaggcaat gcccctgctg ttgttcatag
     2581 gcatcggcgc gatgattgac ttcggcccgc tgctttccaa tcctaagatg ttccttttcg
     2641 gcgcagcggc acagttcggc attttcgcag caatcacgat ttcaacgctt ttgggatttg
     2701 acataaagga cgcagtcgcc gcaggcatga ttggcgcggc agacggcccc accgcaatcc
     2761 ttgtttctca ggtgctccag tcaaagtatg tgggcgcaat cgcggtcgcc gcgtattctt
     2821 acatggcgct cgtccccaca attcagccgg cggttataaa ggccataacc accaaaaaag
     2881 agcgtcttat cagaatgcct tacaatccca agaccgtatc aaagagaacc cgtattctct
     2941 tcccgataat cgttaccgtc atagcaggtc ttgtagctcc cgcctccgtt tcccttgtcg
     3001 gcttccttat gttcggcaat ctcatcagag agtgcggagc gctggataac ctttcaaaga
     3061 cggcgcagaa cgagcttgca aaccttgtaa ctctgcttct cggtattaca atctccttca
     3121 gcatggtggc tgagaagttc gtaacctttg acacgctcaa gataatggtt atcggtctcc
     3181 ttgcgttcgt attcgataca atcggcggag taatgttcgc caagttcctc aatctcttct
     3241 ccaagaataa gattaacccg atgataggcg cggcgggcat ttccgcgttc cccatgtccg
     3301 caagagtagt gcagaagatg gctcttgccg aggataagca gaaccacctt ctcatgcacg
     3361 caatcggcgc gaatgtttcg gggcagatag cctcggtcgt tgcaggcggc gttctgctcg
     3421 gcttcttcaa ataaggaggg gtaccaatgt tactgaattt aatcaactta gcctttgaaa
     3481 taaataaaga aaccgtcctt acatcccttg actttatgtg gaagggcatg ctgggaataa
     3541 tgacggtcat tgttgtcatt actttgatag ttacactgat ggccaaattg gactcaaaag
     3601 ccgagaaaaa aagcggcagt caaagctaaa accaaaaccc gggggtaata ataccttccg
     3661 ggtttaaaaa tgtcctgaat tcgcataata ttgacaatct atgcgcccgg taatataata
     3721 aatatttgtt cggagcggag agctactatc tattgttcgg aggtaattat gagttcagcc
     3781 tatgtcagcc ccttgagcaa gcgatacgca agcaaggaga tgcaagagat tttttccgac
     3841 gattcaaagt tcacaacttg gcgtcggctt tgggttgctc tggcggaagc cgagcagcag
     3901 ctcggaatca acattaccga cgagcaaata gaagagctaa gagccaatat aaacaatatt
     3961 gactacgaaa tggcaaggca aaaggagctt gaggttcgtc acgatgttat ggcgcatgtg
     4021 ctcacctacg gcgaggcatg ccccaaggca aagggcataa tccacctggg cgccacatcc
     4081 tgctatgttg gcgacaacac cgatattata ttaatgaaaa aagcgctgtt gagagttaag
     4141 aaactcctgc tcaacgcaat cgctgccctt tcggattttg ccttaaagta caaggaccag
     4201 cccacccttg cctttacaca tttccagccc gcgcagccca ccaccatcgg caaaagagca
     4261 accctttggc tgcacgacct acttttggat ttcgagcagc tggagttcac ccttaaaaat
     4321 ctgaaactcc tcggctgcaa gggcacaacg ggcacgggag caagtttctt ggagcttttt
     4381 gacggcgacc acgaaaaggt caaaaagctg gaaatgataa tagccgaaaa aatgggcttt
     4441 gacgctgtgt atcccgtcag cggtcagacc tattcgagaa aagtggacta tcaggttctt
     4501 tcggtgcttt cctcaatagc ccagagcgca tgcaagtttg cgggcgacat aaggctgctt
     4561 tcacatttaa aggaagtgga cgagcctttt gagcagcacc agataggctc ctccgcgatg
     4621 gcatacaagc gcaatcccat gaggagcgag agaatcgcct ccctttcaag gttcgtaatc
     4681 gccacagcgg aaaatcccgc ctacaccgcc gcgacccagt ggtttgaaag aacccttgac
     4741 gactcagcaa acaagaggct gagcgttccc gaggctttcc ttgccattga cggcatcctc
     4801 tccctttaca tcaacattgt taagggcatg agggtatata ccaagatggc tgaaaagcac
     4861 cttaacgagg agctgccctt catggctacg gagaatatcc ttatgtactg cgtaaagaat
     4921 aagggcaggg accgtcagga gctgcacgag gctataaggg agcactccgt cgccgcggcg
     4981 tacaatataa aggaatacgg cggagacaac gacctgctcc agagaattct ctccgacagc
     5041 cgcttcggtc ttaccaaaga agagcttgac aacatccttg atgtaagacg ctttacgggc
     5101 agggcaaagg agcagacaga ggaatttatt gaagagtata taaagcctat acttgaaaag
     5161 aataaagagt tcttaggcat tgatatagat atcagagttt aaattatata atagttttag
     5221 gaggaacggg tatgttaacg gcagttgtcg gtataaactg gggagacgaa ggaaagggca
     5281 gaatggttga ccttttcgcc gctgattatg atatcgtcat ccgctatcag ggcggaaaca
     5341 acgcgggtca caccgttatt aacgaaaaag gcaagttcat tcttaacctt cttccgtcgg
     5401 gcatcctttc cgaaacaacc gtcaatgtaa tgggcaacgg aatggttatt gaccttgagc
     5461 accttgttaa ggaaatcgcg tccttaacag aaaagggcat caagataacc cccgaaaatc
     5521 ttaaaatcag cgacaaggca gtaatctgca tgccttatca caagctgctt gactgccttg
     5581 aggaggacag actgggcgac gccaagttcg gctccaccag gagaggaatc gcacccgttt
     5641 actccgacaa atatcttaaa aaggcactca gaatgggcga ccttttctat ttcgacgagc
     5701 tcgaaaagaa actgggtccc ttaatggagt ggaagaacct caccgttcgc gacggctaca
     5761 agcacgagga aattaagccc gaggatatgc ttgagtggct taaaaagtac ggcgagccgc
     5821 tcctgcccca tatctgcgac acgaccgtgt atctcaacaa agcggccgaa gagggcaaga
     5881 acattatgtt cgaggctcag ctcggcgcgc tcagagatat tgacttcggc atttatccct
     5941 atacctcctc atcctccaca atcgccgctt acgcgcccat cggcgcgggc attcccaacc
     6001 gcaagattga tgaggtcata ggaataatga aggcgtattc aagctgcgta ggcgagggcc
     6061 ccttcaccgc cgagcttttc ggtaaagagg gagatgagct cagagaagca ggcggcgagt
     6121 acggagccgc gaccggcaga ccgagaagag tcggcggctt tgatgttgtc gcctccagat
     6181 acggcgccat ggttcaggga gctacatcaa tcgctcttac aaagctcgat gttctcagct
     6241 accttgacaa aatccccgtc tgcgtcgctt acgaaataga cggcgaaata gtccatgact
     6301 tcccgacagg ggcaagactt aacagagcaa agcccgtcta tgaatacttt gacggcttta
     6361 aggaagatat aagccactgc agaaaacttg aggacctgcc cgaaaacgct cttaaatatg
     6421 taaaatacat tgaggagcag gtaggctgta agatcagcta tatttcggta ggcgccaaga
     6481 gagaccagta tattaaggtt gactaaatct ttgcgggagc ctaaacagga aggaaatttg
     6541 catgaaaaat cagcttgtat tggttttgga tttcggcggt cagtataagg aactgatcgc
     6601 aagaagaatc agagaactga atgtcctttc catggttctt ccgggcgaca cacccgttga
     6661 aagaatcaag gaactcagtc ccatcggaat tattatgacg ggcggtcccg acagcgtaaa
     6721 cgcccccgac gcttatctct gctcaaaaga ggtttttgag cttggaattc ccgttctggg
     6781 aatctgctac ggctgccagc tcatagcgca tctcttgggc ggcaaggtcg gcacctgcga
     6841 tgtcagcgag tacggcaccg ttaaggcaaa gctgtcgccc gaatcggttc tttttgagaa
     6901 cattaaaccc gaatcaaagg ttttaatgag ccatacaaac cttgtaacac agcttcccga
     6961 aggttttgtt tccacagcct ccacaaggag ctgcaaaatt gcggccattg agaataagga
     7021 gcgcaaaatc ttcggacttc agttccaccc cgaggttgcc cacaccgaaa acggcaccga
     7081 gattataaag aacttccttt acaatgtctg cggcgccaag ggcgactatg acatggcagg
     7141 ctatatagaa acacagattc aggagataag agagcagacc aaaggcaaaa aggtgctgct
     7201 tgccctctcc ggcggcgttg actcatcggt ttgcgcggcc cttctttccg aggctatccc
     7261 cggaaagctg gtctgcatct tcgttgacca cggctttatg agaaagaatg aggccgatga
     7321 ggtcgaggct gtattcagca agagaaatct ccagtttgta agagtcgatg cttccgagcg
     7381 tttcctcagc aaggtaaaag gtgttaccga ccccgaaaga aagaggaaaa tcatcggcga
     7441 ggagtttata agagtctttg aggagcaggc cgcaaagttc ggcgatgtca gcttccttgc
     7501 gcaaggcacc atctatcccg acattatcga atcgggacac ggcaaggcag ctgtaatcaa
     7561 gagccatcac aatgttggcg gactgcctga agatataaga tttgagggaa tcgtcgagcc
     7621 gcttaaaggc cttttcaagg acgaggtcag aaaggtcggc agggagcttg gtttacctga
     7681 gtccatagtt gaaaggcagc ccttccccgg ccccggtctc gctgtcagag ttatcggcga
     7741 aatcacaaag gaaaagttgg atatactcag agaagcggac gcaatattca gggaggaagt
     7801 cggcaaactc aagaagaagc ccagccagta tttcgcggtg cttaccaatc tccgttcagt
     7861 cggcgttatg ggcgacgaga gaacctatga ctacaccctc gccctcagag ccgtaaatac
     7921 gggcgacttt atgaccgcca catactcaaa aatcccccac agcgttctga ataaaatctc
     7981 aagacgaatc acaaacgaag tcaagggcat taacagagta gtcttcgaca tcaccgacaa
     8041 accccccgcc accatcgagt gggagtagta aaaaaatcac ccctaatcat tacgattagg
     8101 ggttttgttt tgtcattgat taagagatta tcagcacaac gggatacata ggctctgctg
     8161 aggtcataac tttattccct caccgctggg tttccagtcg cattttgccg tgctgccgtc
     8221 gggcagaagt attctgatgt ctttaggctc tttttcgagt ccgattgtaa gcgtttcctc
     8281 cggatctgtg cctaaattta taaacatagc gacgagcctt ccgtcgggag taacgcccgc
     8341 ccgcaggcat atttcattat cgcctacata gtaaacagga agcgaatttg cttctttgag
     8401 aagggacaca aactgcctct tgcggctttc attaaggaat gagaaagcgt caatataatt
     8461 ccacggagtg tcgggcgttc cgcagtacac gaccgtgagc ctgccgtttt tgcgtggcaa
     8521 aaccgtcact gcagggcaaa ctcgctcaat gcccgtctgg gtctggtgat agcaatgcga
     8581 caataccctc accccgtcgg cggtaacttt cagctccttc atttcgtatt gccttgcgca
     8641 catattgccc gagtcttcaa atatctctcc gaatatcgtt ttgccctcat aggacactgc
     8701 cttcacgccc aaaaagcgct caaagcctct ttcgataagc aattccgccg acactgagtc
     8761 aaggaatacg gatgaatcct caaacattct ctcaatctgt tcatcgctca tttgggggat
     8821 aagattttca ctgaggaaca cagccccctc aaatttgttt gagaaataaa aggggagccc
     8881 catcctttca aaaacctttg tcgcccatgc gcagttaagg ttatttttat tgggtctgaa
     8941 atcgagttcg tactgggtca caaagggaac gcagcagccg acgggagtta aatcggcgga
     9001 cagctccgac agtctgtcat agaactttgc gtgctttgcg agtatttccc tgtatgcctt
     9061 gccgctgttt ggctcactgg cgccgaacct tgaaatccag tgtttcgctc cctttgcgcc
     9121 gtcaaataca gagactgcca tgtgggcatg gagatacctt gcgcttttgg cgtatctgtt
     9181 gtagggaacc gtatcggttt cggcaattat ggtgtcgatt ccgtcttccc gcagcttttc
     9241 cttgcagact gagagcctga acatagtaag gctaaagttt ctgttagagc tgggagcgta
     9301 agtgccgtta gggactctga caatgggcga attgcctttg cctgcgaaaa tatgcgccat
     9361 atccttcatg gactcgcaga catagcccgt tgcgcagttt gctccctgaa tcgacgggtc
     9421 aacgctgtca atgccctcac gcatagccct tactattttt ataagggagt catgctgaat
     9481 ctctcttaaa accttcgtgt aagggtcgtc cttttcgtgt gtcaaaacat gctcatacaa
     9541 ttcctcgtgg ctcatttcta cgccagtgcg ccttgtgaat tccgctttat ggagagggca
     9601 gcagcaagcg ttcaccccgc cgcctttgcc tgagcgccac atcatcatgc gcatatcatc
     9661 gtctatcata atcacatcgg gattacagct tgcgatttta gccatcgagt ccttcatata
     9721 ttcgcagaaa tcatcgtcat acgggcagta tacattggga gacgccccgt catccagatt
     9781 tacatatcgc tgaaagggcg cgtgaacaag gggatgccca tgtcccaaaa cagactgcac
     9841 aagaatgcca gtcgggatat tgtaaggctg cagcctctct ttgaatttca aatatgtatc
     9901 gcacagccta cccgccaaat cccatacggg atttccttgc ggcgccagct gcatcataaa
     9961 cagagcgcag gttgtaatgc cgttttcata ctgatactat atatcctcac aaatctcatc
    10021 aacgcgctct atgttcaggt cattgattgt gtataaatgc tccatataaa tctcctttgc
    10081 cgatgaatac tgcccgggtt tactttataa tacatttttt cccaagcccc ttcaagcaat
    10141 ttctcgaaac gcaaaaatcg cccgttttgg ttttcctctt aatcttgtac ggcttgcctt
    10201 aacctatttg gggcttgcgc atagaatgat tagcggaagt taaatgttta gcttctgact
    10261 aaatcctata caagaggaga gaaatatatg gctaatgttg atgtaaatgt caccgcgcaa
    10321 gctttagagg ctaatacgcc atatgaggta gtagatatta catttatcta ttcggacgcc
    10381 acagagcaga acggcaatgt aatctattac ggcgcaactc ttaccgacgg caccgtcagc
    10441 gaggatgtgc tgttcagtta tgctgtcacg cccggcacag atattccggc aagcgtatca
    10501 ttcgcttatg agccgacagt agcttttacg gacaccataa ccggaagcaa cggtaaagta
    10561 tattacactc cttaaagcac agcaaaaatt gaatccgtca gttcttctgg cggattcaat
    10621 tctaatatat aaaatttaag gtgatagccg cgataatgag gtatgcttat gaaagtcatc
    10681 gatataaaat gtcaggattc aataaacagc tctttgaatt gctgtcattt ggaagggccc
    10741 aaagacccat tgcatctggg cagatacaat caaatatatc tgttttttaa tttgcccgcc
    10801 tttttggagc caaggcaaat agactgcgca aagctgattc tttacaaagc tccccctttc
    10861 gggcgcaggg acgaaaatgc ccagtgcgag gaaaacagat attatctgtt ccctctcctt
    10921 gattatttca gcgtatatag ctgcaagttc tgcaaacccg caatcgacac atgccgaagg
    10981 gtagtctttt ataacgacta ctgcaagagc tatacagaaa ttgatattac gagcattgtg
    11041 aaggattggc tggatttcaa catagaaaac aagggtctgc tgcttgtggg cagcggtcat
    11101 tccgagtgtg ttttttatga gtcaaaccgc tcagattttg taggaatgcg cccgactttg
    11161 agattgttct gcagcggaat aagtcccttc ccgactccca cacttgcttg cgccgactgc
    11221 gatgtcgtaa tcaacagacc ctcataaaaa agtttttaaa cggagaaagg tatgtcatta
    11281 ggctatactt agaaacctgc cgcatgacgg aagtttttgc ggcaggcttt ttttatgttt
    11341 ttctgagcca aagtatggca atttgcacaa caggcggata ttttttcctt tcatttttag
    11401 aataatatca aaacttaaaa ttggttattg acaaagtata taaaacatat caatataata
    11461 tacaaagaac ggcgccgaac ataaaaaggc gttaatttat taaaaggagg cacattcatt
    11521 atgtattcag tagtattcag agctttaagg agtaatttca gaaatggccg aatgtgccgg
    11581 tgcaaataac ctttagccct aaaccgaatc ttttatcaaa ttaatctaaa aattattaat
    11641 gaaagaggta tttgcacatg agtaagaaat acagatttga gacacttcag gttcacgcag
    11701 gtcaggaaaa gcccgatccc gcaacagacg caagagcggt tcccatatac gcaaccacat
    11761 cctatgtatt ccccggcgct gaaagcgcgg cagccagatt cggactcaga gaggtcggaa
    11821 atatttattc aagacttacc aaccccacca actctgtttt tgaggagcga atcaacgccc
    11881 ttgagggagg cataggcgcg cttgccaccg catctggctc agcggccata acctacgcta
    11941 ttctgaatat cgccaccgca ggcagtcaca ttgtctccga ccacagaata tacggcggca
    12001 catacaacct ctttgctaac acattaaaag actacggcat tgaaaccacc tttgtatccg
    12061 gcgacaa
//
LOCUS       DAONVQ010000055        12054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4062_length_12054_cov_4.636553, whole genome shotgun sequence.
ACCESSION   DAONVQ010000055 DAONVQ010000000
VERSION     DAONVQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12054
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4062_length_12054_cov_4.636553"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PLD98_04865"
     CDS             complement(<1..993)
                     /locus_tag="PLD98_04865"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQA05614.1"
                     /translation="MGLRLNCAYLDKFVSSADINSMKDRVAKAHELLHTDAADTLGAA
                     WRGWVRLPENYDKDEFRRIKEAAEKIQKNSEVLVVIGIGGSYLGARAAIEFVKSKNYN
                     LIKKDTPDIYFAGNSISSSELAELLTICKDRDFSINVISKSGTTTEPAIAFRLFKALI
                     EKKYGKEAKERIFVTTDKEKGTLKYLADREGYPTFVVPDDIGGRYSVLTVVGLLPLAV
                     AGLDLDALMQGAADAMTEYANPNLSENDCYRYAAIRNILYDKGKSVEMFVSYEPDFAL
                     MNEWLKQLFGESEGKDGKGIFPASAIYSTDLHSLGQYVQDGRKLGLIKDLDLDVE"
     gene            complement(1279..1722)
                     /locus_tag="PLD98_04870"
     CDS             complement(1279..1722)
                     /locus_tag="PLD98_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQA05615.1"
                     /translation="MLAKNIKLFRLYNGYTQQQVADILNVDRSTYAYYESGKSVPDIE
                     TIIKLARLYNISIDFLLGNAEYTETKSDEESGDKLKDSEPNYNKTSETADETLDESGG
                     RADCDVLTVNELDHDERQLIMYFRKLKNKKTVLRLLEALSEKGEI"
     gene            complement(1779..2027)
                     /locus_tag="PLD98_04875"
     CDS             complement(1779..2027)
                     /locus_tag="PLD98_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HQA05616.1"
                     /translation="MRIRQQSLGDRNIIGKRVEQRRKAIKLKQKELLDQLKVRGVDLN
                     SSGLSKLEGQLRRVTDYELVAIADALGVSVNWLLGIDD"
     gene            2514..2891
                     /locus_tag="PLD98_04880"
     CDS             2514..2891
                     /locus_tag="PLD98_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HQA05617.1"
                     /translation="MKMQESGEMYLETILLLTKRGGDVRSIDIVNEMGFSKPSISRAV
                     NLLKKEGYIDIDGAGRITLTEKGLSTATDIYERHQCISKLLQKIGVSPETASKDACRI
                     EHVISEESFEKIKEFIDNENGAN"
     gene            2897..4093
                     /locus_tag="PLD98_04885"
     CDS             2897..4093
                     /locus_tag="PLD98_04885"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQA05618.1"
                     /translation="MNTSVYNSLLERIREIPIVNTHSHHLPDELLISAGLKDILSNSY
                     VNWMSPPPDFDNLNACEEYINKNCSNTYFRWLFKAFEDLYGIKLSKDNFKELDRKIKE
                     AYADNEHHIRILKENCRFEKIVNERQPNPGSNLGRPELFFPSFRCDSFFSAYLKDKPD
                     PNNFFAYSLFSKQEINDITDFLSEVKNAIANKKAEGCVALKVAIAYERPMSFKKTNLL
                     KAQRALNNPQASDEEIIAFGDAVMHTIADTAAELGIPVQIHTGMGQLRDTRPIMLLDF
                     IEAHPDTKFHLLHGGFPWFDDTYALLHNYKNVWSDTCWIPYLSTTAAVDYYIKALEVS
                     DTHRMTWGCDTWMSEDAYGALLAMEHALAKALAIMIEDGAFDFDYAVYIAERILRQNA
                     LELFFA"
     gene            4237..5727
                     /locus_tag="PLD98_04890"
     CDS             4237..5727
                     /locus_tag="PLD98_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HQA05619.1"
                     /translation="MIRKIGFDNDAYIKKQSEQIMRRIDTFGGKLYLEFGGKLFDDYH
                     AARVLPGFLPDTKIKMLQEIKDDLEIVIVINATDIEKNKIRRDLGISYNLDVLRLIDA
                     FRGMGLYVGSVVITQFNSQPNTILYKSKLEQLGVKVFLHYTIDDYPYDISTIVSENGF
                     GRNEYIETTRPLVVVTAPGPGSGKMATCLSQLYHENLRGVKAGYAKFETFPIWNLPLK
                     HPVNLAYEAATADLNDVNMIDPYHLEAYGITSINYNRDVEVFPVLNTIFDKIAGKSPY
                     KSPTDMGVNMAGHCIIDDEAVSQAAKDEIIRRYFAALCDLRKGSGTDMAVQKIELLMK
                     QAGISVDDRIVVGEALNRSANTNNQPAVAIELPDGRIVTGKTSDLLGAAAAALLNAVK
                     ALGGIDKEVHLVDPKLIKPIQKLKIDMLGNQNPRLHSDEVLISLSISAAENEAARIAF
                     NQLSNLRGCEAHSTVILSQVDEDTYRRLGINLTCEPQYQTKKLYHK"
     gene            5741..7357
                     /locus_tag="PLD98_04895"
     CDS             5741..7357
                     /locus_tag="PLD98_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HQA05620.1"
                     /translation="MKLIALSSLSKVFNDEKPTDSGFKSLSMLLNERRSFQVAFQNKA
                     NDSITFSIDSELKGFIKAYLVKCIPSKLAAYPDHDDYVLRNGESGLYPDLLCPIKENT
                     PFSAAEGWNSVWLEIVPDESVKPANYIVKVNIQVGELKAEEEVEVNIIDKLLPAQELI
                     FTNWFHSDCLATYYGVEVLSERHWEIIESFVKTAVEHGLNLLLTPIFTPPLDTAVGGE
                     RPTVQLVDVYRKGYKYSFDFTKLKRWIEMADRNGVKYFEISHLFTQWGAKHAPKIMGC
                     FKGEYVKLFGWETKAESQGYRNFLRQFAEAFNKFVAENNLKDRIFIHVSDEPHMRDLV
                     HYRRAAKLIKEIFPDYRTLDALSDYEFYKKGLVETPVPAENHIEPFVGKVPRLWTYYC
                     CGQYKNYVPNRFFAMPSQRNEILGFLLYKYSCEGFLQWGYNFWYSQYSIREINPFTES
                     DAGGAFPSGDAYVVYPAPDGTPYTSLRLKVFYDGFQDLRLLKLLEKKIGRDEVIKVLE
                     KGLAAPLSFTDYPRSDEWLLNKREEIIRML"
     gene            complement(7389..8690)
                     /locus_tag="PLD98_04900"
     CDS             complement(7389..8690)
                     /locus_tag="PLD98_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HQA05621.1"
                     /translation="MTNKEIAVFTLDALKKAGADHAQCVVTTGLSEEIDAENSEFTKM
                     ISLFDAGVAMKAIKDGKKGTMFINSLNEEDIIKAAEECVAAANASVVDDAVRIAELTE
                     NKSFEKGVLSPDKEKFFFRIKEYVDEIKKTYPQIVLEQLIADYSCAETVFMNTNGVEF
                     NYKNGYYSLTPMFSAHDGEKTTSFNYFEIAFTSLEDSIIELAGQRDIFDQTVKLLDAE
                     AFTGKFEGQAIIVPSCLADFISMAVGNFVSDGTIIDGTSPWRNMLGQKVASDCLTLSV
                     NPLDECMVVGERITDDGYISENYDIIKNGVLESFCLSDYAARKTGESRAKNSSSCYVV
                     APGEQSLDEIIKSTEKGILVCRFSGGSPAINGDFSGVAKNSFLIENGRISKPIAETMI
                     SGNIAAMLKNISGISKEVICDGHTKLPWIRFDGITVSGKVE"
     gene            complement(8718..10100)
                     /locus_tag="PLD98_04905"
     CDS             complement(8718..10100)
                     /locus_tag="PLD98_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HQA05622.1"
                     /translation="MLNDLLKKHISGIPEHAELRAQINTNRGVSIVSGNLVSNVRSQS
                     SGVCARVYKNGVWGFAAKGEYTDEAISDVLTAARNNADFLNTHAGRGKPPLVPVQPPF
                     NPLKRDYVDVPQSAYIDFCRELDDYITNKYKNLASRTVIARADSMEKLLYTSEGGYSH
                     AIMPRAYIYVVLSAETPDGDTVELFEPIGGGEGYFDELYKSPEDCHKEIDKLYEELMQ
                     KREGIFPNAGLRDVVLDATLAGMLAHEAVGHTVEADLVMAGSVASKTLGREVASDKIT
                     MIDFAHTALGKPVPLPVYVDDEGTPCEDAVLIKDGILVGYMHNRESAMYYDVKPTGNA
                     RAHKFSDEPLIRMRNTAILPGKDKLSDMIASIDNGYYLTRTQNGQADTTGEFMFGITM
                     GYEIKNGKLGRALRDTTISGVAFEMLKTVDMVSDDMVWSSSGYCGKKQLMPVSMGGPA
                     IKCKVNIGGR"
     gene            10298..10918
                     /locus_tag="PLD98_04910"
     CDS             10298..10918
                     /locus_tag="PLD98_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQA05623.1"
                     /translation="MEAIYNFDHAVFQFVQKYIWNESILHPIMKVITTLGDGGTIWII
                     IALILLFTKKYRKYGFMMAAGLIITLVVNDNILKPLIARPRPFDFEGWTDFIYPEIIK
                     RPDGFSFPSGHTTSSFLSTVPLLKANKKFGIPALVLAALIAFSRVYVFVHYPTDILGG
                     IVMGFIYGLLGCLIVDQIYKAIERRKMKKRMESKTEEATEETTVNA"
     gene            11037..>12054
                     /locus_tag="PLD98_04915"
     CDS             11037..>12054
                     /locus_tag="PLD98_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQA05624.1"
                     /translation="MPLGQVSVLFKNISILNDFFDVKENMYVGVENGIINFVGEKKPD
                     KKYETEIDGSGKLLAPGFFNLHSHAAMTILRGYAENLPLDRWLNEKIFPFEARLTEQD
                     VYNGVMLAAAEMLRFGIVSFSDMYMFGAATARAVSDSGLKCNLSVPVFCFDDKDADKL
                     PVLNDLDKLMDEFKDSKNIIFELAVHAEYTSTEKAVRNSAEYAKDRNLGVHIHLSETK
                     KEHEECKIRHGKTPAEYFADCGLFDLRTTAAHCVYVEDRDLDILKEHNVSPALNPASN
                     MKLASGFAPVPKMLEKGINICLGTDGAASSNNLNMQKDLYLLSIIYKAAYEDAAAVSY
                     KDCLK"
BASE COUNT         3264 a         2708 c         2678 g         3404 t
ORIGIN      
        1 ttccacatca agatcaagat ccttgatgag tccaagcttt ctgccgtcct gaacatactg
       61 tccgagggaa tgaaggtcgg ttgaataaat ggctgaggcg gggaaaatac ccttgccgtc
      121 tttgccctcg ctttcgccga aaagctgttt gagccactca ttcataaggg caaaatcggg
      181 ctcatagctt acaaacatct caaccgactt gcccttatcg taaaggatgt tccttatagc
      241 cgcatagcgg tagcagtcgt tttctgaaag attggggtta gcatactccg tcatggcgtc
      301 ggcagcgccc tgcataagag cgtcaaggtc aagacccgca acagcaaggg gcaaaagacc
      361 cactaccgta agaacggaat atctgccgcc aatatcgtct ggaaccacga aagtgggata
      421 gccctctctg tcggcaaggt attttaaagt gcccttttcc ttgtctgtgg taacgaaaat
      481 gcgctcttta gcttccttgc cgtatttctt ttcaataaga gccttaaaga gcctgaaggc
      541 tattgcgggc tcagttgttg tgcccgattt ggagataaca tttattgaaa aatccctgtc
      601 tttgcatatg gttaaaagct cggcaagctc tgaggaggaa atggagttgc ccgcaaagta
      661 tatatcggga gtgtcttttt tgattaaatt atagttcttt gatttgacaa actctatcgc
      721 ggctctggcg cccagatatg aaccgccgat tcctatgaca accagaacct ctgaattctt
      781 ttgaatcttc tcagccgctt ctttaattct tctgaattcg tccttgtcat agttttcggg
      841 gagtcttacc cagcctctcc aagcggcgcc taaagtatcg gcagcgtcgg tatgcagaag
      901 ctcatgagcc tttgccactc tgtccttcat agagtttatg tctgcggagc taacaaattt
      961 gtcaagataa gcgcagttta gtcttaatcc catcagaaaa atctccttca agaaaaaagt
     1021 aaaacgatta gaaaaatcaa taaaaacgat attttgatta gtgaaatgta ctatttaccc
     1081 attatcttgt atcgttttta cagttaaatt gtactatatt aaaaaattct tttcaataga
     1141 tagtttgctc agataatgcc gataaaaact atattggttt agctttctgc cgacccgaat
     1201 gcgcatttat ttacatacat tgcattaaat acaaaaggct ttaataacaa gaaaagggct
     1261 gccttacggc accctttttt aaatttcacc tttttcagaa agagcctcaa gaagacgcag
     1321 gacggttttt ttgttcttca gctttctgaa gtacattata agctgacgct catcgtgatc
     1381 aagctcgttt actgtaagaa catcgcagtc agcgcgtcct ccgctttcgt ccaaggtctc
     1441 gtccgcagtt tccgaagtct tgttgtaatt gggctcactg tcttttaatt tgtcgcccga
     1501 ttcttcatcg ctcttggttt cggtgtattc cgcattgcct aagagaaaat caatgctgat
     1561 gttatagagc cttgcaagct ttataatagt ctcaatatca ggtaccgatt tgcccgattc
     1621 gtagtaagca tatgtggaac ggtcaacatt taaaatatca gcaacctgct gctgagtgta
     1681 gccgttatat aatctgaaaa gtttaatatt tttggcgagc aaatcagtgt cccccctgat
     1741 gtatttcggt aaatgcattg gctgaattaa tatgtatgtt aatcatctat gcctaaaagc
     1801 caattaacac ttactcctaa agcatcagct atggcgacaa gctcgtaatc ggttacccgc
     1861 cttaactgtc cttcaagctt tgacagtccc gaggaattta aatccactcc cctgacttta
     1921 agctgatcca gcagttcctt ttgcttaagt ttaatggctt tccttctttg ttcaactctt
     1981 ttgccgatga tgttgcggtc accgaggctc tgttgtctga tcctcatttg caatcctcca
     2041 aaattctttc cattatcatt cgtgaaaaga aatataacat ggcagtattg caaattcaga
     2101 aataaattgt gaacagttta acaaaaatct tgagataagt tgttaattat tagcttttag
     2161 gcaacaaatt ttctattaat tccctttttt gatgctttta gtcactatgt gtataatctt
     2221 tcaacttatt gcttttcatc ttatcactat attaaagtga tgtcaattat ttaaacactg
     2281 agctaaaatt ttcggctttt ctaacagaaa tcatacaaaa acaaaggcat ttttttacat
     2341 tttgttaaaa gaaagtttac atttaaagag ttatttttga cagttttaat gtattatggc
     2401 aattttgctt gcaatatatt ataaaaatta tataataaat agttgatggc aataaatagc
     2461 gccttattta gattaaatat acggttcaat aattgcataa caggaggagt attatgaaaa
     2521 tgcaggaatc cggcgagatg tatctggaaa ccatactact gctcacaaaa cgcggcggcg
     2581 atgtccgctc tatagatata gttaacgaaa tgggcttttc taagccgagc atcagcaggg
     2641 ctgtaaatct cttgaaaaag gaaggctaca tagatattga cggcgcggga agaatcactc
     2701 ttaccgaaaa gggtcttagt accgcgaccg atatttatga aaggcatcag tgcatctcaa
     2761 agctcctgca gaaaatcggc gtcagccccg aaaccgcctc aaaggacgcc tgccgaattg
     2821 agcatgttat aagcgaggaa tcttttgaaa aaattaaaga gttcatagat aacgaaaacg
     2881 gagctaacta gagagcatga atacatctgt ttacaatagt ctgttagaaa gaatcagaga
     2941 aatcccgata gtaaatactc attcgcacca tttgcctgat gagcttttaa tctcagcggg
     3001 tcttaaagat attctgtcaa acagctatgt taactggatg tcccctcctc ctgattttga
     3061 taatcttaat gcttgcgaag aatatataaa caaaaactgc tctaatacat atttccgctg
     3121 gcttttcaag gcttttgagg atttgtacgg cataaagctc agcaaagaca atttcaaaga
     3181 gcttgaccgg aaaattaaag aggcttatgc cgataatgag catcatataa gaatcttaaa
     3241 ggaaaactgc cgttttgaga aaattgtgaa tgagcgtcag cccaaccccg gcagcaattt
     3301 gggtcgtcct gaactctttt tcccaagctt tagatgcgac agcttttttt cggcatacct
     3361 aaaggacaag cccgacccca ataatttctt tgcctattct cttttctcca agcaggaaat
     3421 taacgatata accgactttt tgtctgaggt aaaaaacgcg atagcaaata aaaaggcgga
     3481 aggctgcgtc gctttgaagg tggcgatagc ctacgaaaga cccatgtcct ttaaaaagac
     3541 aaatctatta aaggctcaaa gggctcttaa caatcctcag gcaagcgatg aagaaatcat
     3601 tgcattcggc gatgctgtta tgcacactat tgccgataca gccgctgagc ttggtatccc
     3661 agttcagatt cacacgggca tgggtcagct gagagacaca agacccatta tgctccttga
     3721 ttttattgag gctcatcctg acaccaaatt ccatcttctc catggcggtt tcccgtggtt
     3781 tgacgacacc tacgctctgc tgcacaacta taagaatgta tggtccgaca cctgctggat
     3841 tccctacctt tccaccactg cggctgtcga ttactacatt aaggctcttg aggtatccga
     3901 tacccacaga atgacatggg gctgcgacac ttggatgagc gaggacgctt acggcgctct
     3961 tttagccatg gagcatgctc ttgcaaaggc tcttgcgatt atgattgagg acggagcctt
     4021 cgactttgac tatgcggtct atattgctga gagaatttta aggcagaacg ctttggagct
     4081 gttctttgca taaaaagcag atttgtgctt aacaagattg tttttatgtg atacaatctt
     4141 ttggattttg gatttttgag agccgcacat atcgcagctt tagctgtgta aatgcggtat
     4201 atctttttgt ttttaaatag attcgtgggg gttcatatga ttcgcaaaat cggttttgac
     4261 aacgatgctt acataaagaa gcaatcggag cagattatga ggcgcattga tactttcggc
     4321 ggcaagctgt acctcgagtt cggcggcaag ctgtttgacg actatcacgc cgcaagggtt
     4381 ctgcccggtt tccttcccga tacaaaaatt aagatgctgc aggaaatcaa ggacgacctg
     4441 gagattgtca tagtaattaa cgcgactgac attgaaaaga acaaaatccg ccgcgacctc
     4501 ggcatctcat acaacctcga tgtattaagg cttatcgacg cgttccgagg catgggtctt
     4561 tatgtgggca gcgttgttat aactcagttc aattctcagc ccaacaccat tctttacaaa
     4621 tctaagctgg agcagcttgg ggtaaaggtt ttcctgcatt acaccataga cgactatccg
     4681 tatgacatca gcacaatagt cagcgaaaac ggttttggca ggaacgaata catagaaaca
     4741 acaaggccgc ttgttgtggt taccgccccc ggtcccggca gcggaaagat ggcaacatgc
     4801 ctttcacagc tttatcatga gaatttaagg ggcgttaagg caggatacgc aaaatttgag
     4861 accttcccta tatggaatct gccattaaag caccccgtaa accttgctta tgaggccgca
     4921 accgccgacc ttaacgatgt aaacatgatt gacccgtatc accttgaggc atacggcatt
     4981 acaagcataa attacaacag ggatgttgaa gtcttccccg tacttaacac catatttgat
     5041 aaaatcgcgg gcaaaagccc gtataaatca cctacagaca tgggagtcaa tatggcagga
     5101 cactgtatta ttgatgacga agcagtaagt caggctgcga aagatgagat tatacgccgc
     5161 tactttgccg ctctttgcga tttgcgcaag ggctcgggca ccgatatggc tgttcagaaa
     5221 attgagctgc ttatgaagca ggcgggaatt tcggttgacg acagaattgt tgttggcgag
     5281 gctcttaacc gctccgcaaa tacaaacaat cagcccgccg tcgccataga gctgcccgac
     5341 ggacgcattg ttacgggcaa aacctccgac cttttgggcg ccgccgctgc ggctctgctc
     5401 aatgcggtca aggcactggg cggaatcgac aaggaggttc accttgttga ccccaaactt
     5461 attaagccta ttcagaaatt aaagatagat atgctgggca atcaaaatcc ccgacttcat
     5521 tccgatgagg tgctgatttc cctttctatc agcgcggcgg aaaacgaagc cgctagaatt
     5581 gcctttaacc agctttcaaa tctcagaggc tgcgaggctc actctacagt tattctctct
     5641 caggttgacg aggatacata ccgcaggctc ggcataaatc ttacttgcga acctcagtat
     5701 cagaccaaaa agctttacca taaataaatc ggaggtttgt atgaagctaa tagctttaag
     5761 ttctctcagc aaggttttta atgatgaaaa accgactgac agcggcttta aatccctatc
     5821 aatgctttta aatgagcgcc gttcttttca ggtcgccttt caaaataagg cgaacgacag
     5881 cattactttc agcattgact cagagctgaa gggctttatc aaggcgtatc tggtaaaatg
     5941 catcccctca aagctggcgg cgtaccccga ccacgacgat tatgttttga gaaacggcga
     6001 gagcgggctt tatcccgatt tgctctgccc cataaaagaa aacacgccct tctcagccgc
     6061 ggagggctgg aacagcgttt ggcttgagat tgttcccgat gaaagcgtca agcccgcaaa
     6121 ctacatagta aaagtaaata ttcaggttgg agaacttaaa gctgaggaag aagttgaagt
     6181 aaatattatt gacaagctgc tgcccgcgca ggagcttata ttcacaaact ggttccattc
     6241 agactgcctt gccacatatt acggcgttga ggttttaagc gagaggcact gggagattat
     6301 tgagagcttt gttaaaaccg ctgtggagca cggtttgaat ctgcttctta ccccgatttt
     6361 caccccaccc cttgataccg ccgtcggcgg cgagcgtccc acggttcagc ttgtggatgt
     6421 ttaccgcaag ggctataaat acagctttga ctttacaaaa ctcaaacgct ggattgagat
     6481 ggcggacaga aacggggtta aatactttga aatttcccac cttttcaccc agtggggcgc
     6541 aaagcacgcg ccgaaaatta tgggctgctt taagggcgaa tatgtaaagc tcttcggctg
     6601 ggaaacaaag gccgaaagtc agggctacag gaacttttta agacagtttg ccgaggcttt
     6661 caataaattt gtggcggaga ataatcttaa agacaggata ttcattcatg tttccgacga
     6721 gccgcacatg agggatttgg tacactaccg cagagcggca aaacttatta aagagatttt
     6781 ccccgattac aggacccttg acgctctttc agattatgag ttctacaaaa aggggcttgt
     6841 ggagaccccc gttcccgccg agaatcatat tgagcccttt gtgggcaagg ttccgaggct
     6901 gtggacatac tactgctgcg gacagtataa aaactatgtg cccaacagat tcttcgcaat
     6961 gccctcacag agaaacgaga ttctgggctt tttgctttac aaatacagct gcgagggttt
     7021 tctgcagtgg ggatataact tctggtattc ccagtactca atcagggaga ttaacccctt
     7081 taccgaatcc gacgcgggcg gagcgttccc atcgggtgac gcttatgtgg tatatcccgc
     7141 tcccgacgga acgccttata catctctgag actcaaggtt ttctatgacg gtttccagga
     7201 tttaaggctt ttaaagctgc ttgagaaaaa aatcggcaga gatgaggtta ttaaggttct
     7261 tgaaaaaggt cttgccgctc ccctttcatt taccgattat ccaaggagcg atgagtggct
     7321 cttaaacaag cgtgaagaaa taataaggat gctctaataa taaaacccct ctttcgaggg
     7381 gtttcttttt attcaacctt gcccgaaacg gttatgccgt caaagcgtat ccacgggagc
     7441 ttggtgtggc cgtcgcagat aacttccttt gaaattcccg aaatattctt cagcatggcg
     7501 gcgatgttgc cgcttatcat ggtttctgca ataggctttg agattctgcc gttttcaata
     7561 aggaagctgt ttttggcgac gcctgaaaag tcgccgttga ttgcgggtga accgcccgag
     7621 aaacggcaaa ccagaatgcc cttttcggtg ctcttgatta tttcgtcaag agactgctct
     7681 ccgggggcga ccacatagca gctgctggag ttcttggctc tgctctcgcc cgtctttctt
     7741 gccgcatagt cggaaagaca gaaagactca agcactccgt tctttataat gtcatagttt
     7801 tcgcttatat agccgtcatc ggtaattctc tctcccacaa ccatgcactc gtccaaggga
     7861 tttacagaga gtgtaaggca gtcggaggcg accttttggc ccagcatatt acgccacggg
     7921 cttgttccgt caataattgt gccgtcggag acaaaattgc ccacagccat tgagataaag
     7981 tcggcaaggc aggagggaac aataatagcc tgaccctcaa actttcctgt aaaggcttca
     8041 gcgtcaagca gctttacggt ttggtcaaat atatcccgct gacccgcaag ctctatgatg
     8101 ctgtcctcaa gtgaagtaaa ggcaatttca aaataattga acgatgtggt tttctctccg
     8161 tcatgggctg agaacatggg agtgagtgaa tagtagccgt tcttgtaatt gaactcaacg
     8221 ccgtttgtgt tcataaagac ggtttcggcg caggagtagt cggctataag ctgttctaag
     8281 acaatctgcg gataggtttt cttaatctcg tccacatatt ccttgattct gaagaagaac
     8341 ttttccttgt cgggacttaa tacgcctttt tcaaagctct tgttttctgt cagctccgca
     8401 atgcgcacag cgtcatccac aacggaggcg ttggcggccg ctacgcattc ctcggcagct
     8461 ttgattatat cctcttcgtt cagcgagttt atgaacatcg tacccttttt gccgtctttt
     8521 atagccttca tggcgactcc cgcgtcaaaa agggaaatca tcttggtaaa ctcgctgttt
     8581 tccgcatcta tttcttccga cagacccgta gtaacgacgc actgggcatg gtctgcgcct
     8641 gcttttttca gggcatcaag agtaaatact gcaatttctt tattagtcat acggacatcc
     8701 ctttcataaa atggctttta tctgccgcct atattaacct tgcacttgat ggcggggccg
     8761 cccattgaaa cgggcataag ctgctttttg ccgcagtatc ccgatgaact ccagaccata
     8821 tcgtctgaca ccatatccac agtttttagc atttcaaagg caacgccaga aattgtggta
     8881 tctcttaagg ctctgccgag ttttccgttt ttaatctcat agcccatggt gatgccgaac
     8941 atgaattcgc cggtggtgtc tgcctgaccg ttctgggttc tggtaagata atatccgttg
     9001 tctattgagg caatcatatc cgacagcttg tccttgccgg gcagaattgc tgtgtttctc
     9061 attctgataa ggggttcgtc agagaattta tgcgcccttg cgtttcctgt gggctttaca
     9121 tcatagtaca ttgcgctctc tctgttgtgc atatagccca caagtatgcc gtcttttata
     9181 agcaccgcgt cttcgcaagg tgttccctcg tcgtccacat agacgggcag cggaacgggc
     9241 ttgcccaaag ctgtatgggc aaagtctatc attgtgattt tatccgaggc tacctctctg
     9301 ccaagggttt ttgaggctac ggagcctgcc atgacaaggt ctgcctctac ggtgtgacct
     9361 accgcttcgt gggccagcat tcccgcaagg gtcgcgtcga gaaccacatc tcggagaccc
     9421 gcgttgggga atatgccttc ccttttctgc attagctcct catacagctt gtctatttcc
     9481 ttgtggcagt cctcggggct cttgtaaagc tcatcgaaat agccctcgcc gccgcctatc
     9541 ggctcaaaaa gctcgacggt gtcgccgtcg ggggtttcag ccgacagcac cacatagata
     9601 tatgcgcggg gcattatggc atgggaatag ccaccttcgc ttgtgtaaag gagcttttcc
     9661 atactgtccg cccttgctat tacggtgcgg ctcgcaaggt ttttgtattt attcgtaata
     9721 tagtcgtcaa gctccctgca aaagtcgata tacgcgcttt ggggaacatc cacataatct
     9781 cttttgaggg ggttgaaggg gggctgaacg ggtactagcg gaggcttgcc cctgcctgcg
     9841 tgggtgttaa ggaaatcggc gttattcctt gccgctgtga gcacatccga aattgcctca
     9901 tccgtgtact cacctttagc ggcaaaaccc caaacgccgt ttttgtaaac ccttgcgcaa
     9961 acacccgagc tctgtgagcg gacattggac acaaggttgc cgctgacgat tgaaactcct
    10021 ctgtttgtat ttatctgcgc tcttaattct gcgtgctcag gtatgcctga gatatgcttc
    10081 tttaaaaggt cattcagcaa aatatcatct ccaaatgaaa cgatttggtt tcaagtacta
    10141 gtatatccaa tatttgtata ataccacaaa ttaatagcat aaacaatgat gttataatgc
    10201 cgtctttcat aagattttag gcttattgac taagattaga atatatatta ttatggataa
    10261 aactgtgctt tttattttct tttaattgga ggatattatg gaggctattt acaattttga
    10321 ccacgctgta tttcagtttg tccaaaagta catatggaac gagtcaatac tccaccccat
    10381 aatgaaggta ataaccactt tgggcgacgg cggcaccata tggattatta ttgccctaat
    10441 tctcttattt accaaaaaat atcgcaaata cggctttatg atggctgcgg gtcttataat
    10501 aacccttgtt gtaaacgaca atatcttaaa gccccttata gcaagacctc gtccctttga
    10561 ttttgaggga tggaccgatt ttatttatcc cgaaataatt aaaagaccgg acggtttctc
    10621 cttcccctcg ggccatacaa cctcctcctt cctttccact gtgccgcttt tgaaggcgaa
    10681 taaaaagttc ggaattcccg ctttggttct ggcggctctg attgccttct caagagtata
    10741 tgtgttcgtt cactatccca cggatattct tggcggcatt gtaatgggct ttatttacgg
    10801 tcttctcggc tgccttatag ttgaccaaat ttataaggca atcgaaagaa gaaaaatgaa
    10861 aaagagaatg gaaagcaaga cagaagaagc aacagaagaa acaaccgtta acgcataaaa
    10921 aaaagagcgg aaggctgcgg cttttcgctc tttttttgtt tactccgccc cgctttaatt
    10981 gaatttaaag gaacgctgtg atagaataat ttaacaattc aaaaggggga attcttgtgc
    11041 ctctgggaca agtatcggtg ctatttaaga atataagtat tctcaatgat ttttttgatg
    11101 ttaaagagaa tatgtatgtc ggcgttgaga atgggattat taattttgtt ggagaaaaaa
    11161 aacctgataa aaaatacgaa accgaaattg acggcagcgg aaagctgctg gctccgggct
    11221 tttttaatct ccactcccac gccgcgatga cgatacttag gggttatgcg gagaacctac
    11281 ctttggacag gtggcttaat gagaagattt ttccctttga ggcgaggctc actgagcagg
    11341 atgtttataa cggagtaatg ctcgccgccg cggagatgct gagattcggc attgtgtcgt
    11401 tctcggatat gtatatgttc ggcgccgcta cggcaagggc ggtcagcgat tcggggctaa
    11461 aatgcaattt gtcggtaccc gtattctgct ttgacgataa ggacgcggac aagctccccg
    11521 ttttgaatga ccttgataaa ctgatggatg agtttaaaga ctcaaagaat attatttttg
    11581 agctggcggt gcatgcggaa tatacatcga ccgaaaaggc tgtaaggaat tccgctgaat
    11641 acgcaaagga taggaatctg ggcgttcata ttcacctttc tgaaacaaaa aaggagcatg
    11701 aggagtgcaa aatccgccac ggcaaaactc ccgccgagta ttttgcggac tgcgggctct
    11761 ttgatttaag gacaaccgcc gcccactgcg tttatgtgga agacagagat ttagatattc
    11821 taaaggagca caatgtctcc cccgctttaa acccagcaag caacatgaag ctggcaagcg
    11881 gctttgcacc cgtgccgaaa atgcttgaaa aaggaatcaa catctgcctt ggaaccgacg
    11941 gagccgcaag cagcaacaat cttaatatgc agaaggattt ataccttttg tcaataatct
    12001 ataaggcggc ttacgaagac gccgccgccg tcagctacaa agactgtctg aagg
//
LOCUS       DAONVQ010000056        12019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4081_length_12019_cov_5.696339, whole genome shotgun sequence.
ACCESSION   DAONVQ010000056 DAONVQ010000000
VERSION     DAONVQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12019
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4081_length_12019_cov_5.696339"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLD98_04920"
     CDS             <1..624
                     /locus_tag="PLD98_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HQA05625.1"
                     /translation="VKAKAEAEFIEKRSRFIASCAPVKSSKEALEFIESLRKKHWDAA
                     HNVYAYVIKEEGVQRFSDDGEPQGTAGMPVLDVLLKNGVTDCVIVVTRYFGGILLGAG
                     GLVRAYSKSAKLGLDAAGLIEMRDCTSFSVVCDYSFYGKVEAFIRDSGGIIEDTRFTD
                     NVEIDFVIENTKKYKFLKDFPDVTLGRFTPIEKGSVYKAFDLNLNES"
     gene            725..1723
                     /locus_tag="PLD98_04925"
     CDS             725..1723
                     /locus_tag="PLD98_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HQA05626.1"
                     /translation="MPSIREQTLEYERQFLSDKAFHCSYTKGRLREEKECPVRTAFQR
                     DRDRIIHSNAFRRLKDKTQVFLSPEGDHYRTRLTHTLEVSQIARTITRALRLNEDLTE
                     AIALGHDLGHTPFGHAGERALDSVYDGGFRHYEQSLRVVDFLERDGMGLNLSYEVRDG
                     ILCHTTGKMPETLEGRVVRIADKIAYINHDIEDGIRAGIMSEDDIPKYISNVLGKSKS
                     ERIETLVTSLIENSKNDINFSSEVLEAFNSLHEFMFKCLYTNPKCKGEEGKAVILIQK
                     LYEYYCENPDKLPEFYRKISIKEGVGRAACDYISGMSDRYCKTIYTRLFIPHFWEE"
     gene            1747..3504
                     /gene="dnaG"
                     /locus_tag="PLD98_04930"
     CDS             1747..3504
                     /gene="dnaG"
                     /locus_tag="PLD98_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQA05627.1"
                     /translation="MARFSDDFLFELRSRIDIEELISSYVRLKKQGRISRGLCPFHVE
                     KTPSFTVYSDTQSYYCFGCGAGGESITFIRNIENLSYYDAVQFLAEKVGLKMPEETAD
                     DGLYALRKRIFEANREAARFFYANLIKPDCPAYTYLIGRGIKPETIKRFGLGYAPDSW
                     DALTRHLRSKGFKENELISADLSQKSRTGGLIDVFRNRLMFPIIDLKGNVIAFGGRVL
                     GDEKPKYINTSDTPVYKKGKGIFALNIAKAHGRSLILCEGYMDVIAMHQAGFQNAVAG
                     LGTAFTSEQAQILSRYADEVILAYDSDDAGKKAAKRTMSIFQSMNVKTKILTLTGGKD
                     PDEIIKRFGREYMQKIIDGSLSSTEYKLALAKEGINTDTADGKVEYLKKAVEVLSELD
                     DAIERDVYAGRLSEETGVTRTAIDEQIKRALSFRKRKQDIDSFNKAREILEGKVIAAK
                     NALKLYNQSASLRCRQAEETLLASLIQNPEFYKKLKDKITIDDFSSALHKRLFFKITE
                     RIENNQELDLIYFADEFNPEEMSILTKVFQKGRTISNTLDECLDCIKVMSEEKLKQSA
                     ADPSKLSDEEFLRLFYKKE"
     gene            3546..4634
                     /gene="rpoD"
                     /locus_tag="PLD98_04935"
     CDS             3546..4634
                     /gene="rpoD"
                     /locus_tag="PLD98_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HQA05628.1"
                     /translation="MDNNEKRAFVNMLIDKGKAEGRLTAQEIDAAIVEMDFDIDELDK
                     LYETIEQNNIEIIDDMSDAALDPSLFDTEAVKSADASGDLKGLAMDDPVKVYLKEIGK
                     VPLLTPEEEIELAIRIANNDQEAKKRLQEANLRLVVSIAKRYSGRGMQFLDLIQEGNL
                     GLIKAVEKFDYTKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKVKKTNS
                     QLLHKNGRDPTAEEIAEELNMPIEKVREILTFAQEPVSLETPIGEEEDSHLGDFIPDD
                     DALAPADAASQLLLKEALVSVLKTLTPREAKVLSLRFGLEDGHSRTLEEVGKEFNVTR
                     ERIRQIEAKALRKLRHPQRSKRLRDFLD"
     gene            4729..6327
                     /locus_tag="PLD98_04940"
     CDS             4729..6327
                     /locus_tag="PLD98_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05629.1"
                     /translation="MIRLNELKLPIGASEEDVKSLAAKELNIPVSELKDFRIARMSID
                     SRNKERIRLTFSAEFTVDGDESSLISDFPPNRASVFEPYEYTPPAVNRTSPLRPVIVG
                     FGPAGMFAALLLSKAGLKPIVLERGERVEDRTETVKRFWKNRALNTDSNVQFGEGGAG
                     TFSDGKLTTGIKDPRVRYVLSTLAEHGAPKEILYSAKPHIGTDLLANVVRDIRETVIK
                     NGGEIRFGCRLTDLIISNGAVFGVRYLENGKAEREIETDCVLLCIGHSARDTFEMLLG
                     KGVKMQKKPFSVGVRIEHPQELINKMQYGREWNNPLLGAADYKLSNHPKHGRGGYTFC
                     MCPGGVVVTASSEEGMLVVNGMSEYARDGENANSAILIGVEPDYFESEHPLSGIEFQR
                     ELERKAFLLGGGDYTAPAQLVGDFIKDRPSAKLGAVKPSCTTGVTMSDIRKVLPDNIT
                     APLKDALFSFDRQFKGFLLPDAVLTAVESRSSSPVRILRDDLYQSNIRGLYPCGEGAG
                     YAGGIVSAAVDGLRCAEAVLKDET"
     gene            complement(6362..7342)
                     /locus_tag="PLD98_04945"
     CDS             complement(6362..7342)
                     /locus_tag="PLD98_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA05630.1"
                     /translation="MKTIRWGIVGAGNIANRFAEACNNTPGAELCAVASRDIAKAEQF
                     AKKYNIPHAFGSYEEMAAFDGIDIAYIATPHGLHADNAILFMNHKKAVLSEKPITLNM
                     SELERMIASAKENDVFLMEAMWARFVPGTKKLLEIVESGSLGKIRGIQSTFSYDMSDE
                     PDHHAFDPKYGGGSILDVGCYCLSFASWYANSEPEQITAVAELSDKGVDLHCCYLIKY
                     KNGAIASLSSGMTLRKPNEGYLFGEKGYAYVERSYAPQKIELHIEGKDVEVIDCPYYG
                     NGFEEQIMEASECLRQGKKESDIMTHDQSRLIMRLMDEIRRQVGVKYPVD"
     gene            7419..8264
                     /locus_tag="PLD98_04950"
     CDS             7419..8264
                     /locus_tag="PLD98_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HQA05631.1"
                     /translation="MKTGVIDIGGGMRGIYAAGVFDAFIDFGIKFDYCIGVSAGTANL
                     VSFLAGQKGRNYRFYVRHAQDKRYMSLENMIKKGEYFDLSYIYDTLTNHVDPLDYDAL
                     INSPSELKIVATNAKTALPEYFDKEVFLERSCKALMASCALPIACRAVKINDVEYFDG
                     GVSDPIPIRKAIEDGCDRVVVLLPRNVNLRKKPESLRYVYLRALRKYPEIIEAINQRH
                     EKYNRAIDELIELREQGKAVIISPAVQINARINSKNPALLDRLYKQGRRDAVKVLPEI
                     RKWGL"
     gene            complement(8311..9384)
                     /locus_tag="PLD98_04955"
     CDS             complement(8311..9384)
                     /locus_tag="PLD98_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA05632.1"
                     /translation="MSKKLKIAVIGTGSISREHLDAYKENPNVEIYALCDINKERVEK
                     RAAEYNVPLERTFTDKDEMFKALPEIDAVSVCTWNSAHAECSIAALNAGKHVLCEKPM
                     AMNEEEAKEMKAAADKAGKLLMIGFVRRHGNDCALLKDYIDKGYLGEIYYAKAQYLRR
                     NGNPGGWFGDKSRSGGGPLIDLGVHVIDLVRYLMGNPKPISVYGATFQKLFNRPHIKG
                     KKGYTSAEAGNNDICDVEDLATALIRFDNGAVVSLETSYSLNLKEGKGDIELFGTKGG
                     AIIKPQVELYTELNDYMVNIEFPHDTALSFKGLFANEINHFVDCILNGTPCLAPAQDG
                     VDIMRILDSVYKSAELGHEVVLD"
     gene            complement(9519..10262)
                     /gene="sleB"
                     /locus_tag="PLD98_04960"
     CDS             complement(9519..10262)
                     /gene="sleB"
                     /locus_tag="PLD98_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HQA05633.1"
                     /translation="MKPKNKSSKKVSPISKAFYFVTVTALIFSVAYGIFAVSDSYTAI
                     YDGSIEEIAADDTLSKLGSRGDEVRRIQKKLKELGYYKGNVDGIYGVMTQNAVKAFQR
                     AVGIKVDGIAGPQTLLYLGLGGGGGGQFSSSDMYLLARVIGAEARGESYTGQVAVGAV
                     IINRVRHPSFPDTIGGVVYQPGAFSCVYDSNWSVAPNATAKKAAQDAVNGWDPTGGAI
                     YYYNPAKTSNQWIRKRPVTVTIGSHVFCK"
     gene            10852..>12019
                     /locus_tag="PLD98_04965"
     rRNA            10852..>12019
                     /locus_tag="PLD98_04965"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     assembly_gap    11772..11891
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3374 a         2816 c         2941 g         2768 t
ORIGIN      
        1 gttaaagcca aggcagaagc agagtttata gaaaagcgtt caaggtttat agcaagctgc
       61 gcccccgtga aaagctcaaa agaggctttg gaatttattg agtctctcag aaaaaagcac
      121 tgggacgcag cccacaatgt ttatgcctat gtaataaagg aagaaggcgt gcagcggttt
      181 tccgacgacg gagagcccca gggaaccgcg ggtatgcccg tgcttgatgt tcttttaaag
      241 aacggcgtca ccgactgcgt tatcgtggtg acaaggtatt tcggcggaat tctcctcggt
      301 gccggcggtc ttgtcagagc ttattcaaag tccgcaaagc tggggcttga tgccgccggg
      361 cttatcgaaa tgcgcgactg cactagcttt tcagttgtct gcgactacag cttttacggc
      421 aaggtcgaag cgtttataag agacagcggc ggcattatcg aagacacccg attcaccgat
      481 aatgtggaaa tcgacttcgt aatagaaaac accaaaaagt acaaatttct taaagatttt
      541 cccgatgtca ctttgggcag attcactcct atagaaaaag gttcggttta caaagcgttc
      601 gaccttaatt tgaatgaatc ttgaggaaaa aaataataat taaaaaatta aagggaaaaa
      661 ggcatagctt gtcgtatata gtaaatggag cttgattggg attattttag aaaggttgtt
      721 gctgatgccg tcaattagag agcagacact tgaatacgag cgtcaatttc tctccgacaa
      781 agccttccac tgcagctaca caaagggaag gctgagggaa gagaaggaat gtcccgtcag
      841 aacagcgttc cagcgggaca gggacaggat aatccactcc aacgctttca ggcggctaaa
      901 ggacaaaact caggttttcc tctctcccga gggcgaccac tacagaacca ggcttaccca
      961 tacgcttgag gtctcccaaa tagcaaggac aatcacaaga gctttaaggc tcaatgagga
     1021 tttgacggag gctatagccc tcggacacga cctcggccac acccccttcg gacacgcggg
     1081 ggagagagcc ctcgacagcg tctatgacgg cggcttcagg cactatgaac agagcctcag
     1141 agtagtcgac ttccttgagc gtgacggcat ggggctgaat ctgtcatacg aggttcgcga
     1201 cggcatactc tgccacacaa cgggcaaaat gcccgagact cttgagggca gagtagtcag
     1261 gatagccgac aaaatcgcct acataaatca cgatattgaa gacggcataa gggcaggcat
     1321 tatgtccgag gatgatattc cgaaatacat ttcaaatgtt ctcggcaaaa gcaaatccga
     1381 aagaatcgaa actttagtca cctcccttat agaaaacagc aagaacgata taaatttctc
     1441 gagtgaggtt ctggaggcgt tcaacagcct tcatgagttt atgttcaagt gcctttacac
     1501 caaccccaag tgcaaggggg aggagggcaa ggcggttatt ctcattcaaa agctctatga
     1561 gtattactgc gaaaatcccg acaagctgcc cgagttttac agaaaaatct ccataaaaga
     1621 gggcgtgggc agagccgcct gcgactatat ttcaggaatg tcggacagat actgcaaaac
     1681 aatatacaca aggctcttca taccccactt ctgggaggaa taaggcaaaa gaaaggaggg
     1741 ttttaggtgg caaggttcag cgacgatttt ctgtttgagc tgagaagcag aatcgatatc
     1801 gaggagctga tttcctccta tgtgcgcctt aaaaaacaag ggcgaatctc ccgcggcctt
     1861 tgcccctttc atgtagaaaa aaccccctcc tttaccgttt acagcgacac ccagtcctac
     1921 tactgcttcg gctgcggagc cggcggagag agcataacct ttattagaaa cattgaaaac
     1981 ctcagctact acgacgcggt ccagttcctt gccgaaaagg tagggctcaa aatgcccgag
     2041 gaaaccgccg acgacgggct ctacgccctc agaaaacgca tttttgaggc taacagagaa
     2101 gcggcaagat tcttttacgc aaatctaata aagcccgact gtcccgctta tacataccta
     2161 atcggcaggg gaatcaagcc cgaaaccatt aaaagatttg gtctgggcta cgcgcccgac
     2221 tcatgggacg ccttgacgcg gcatctaagg tcaaagggct ttaaagaaaa tgagcttata
     2281 agcgccgacc tttcccaaaa atcccgcaca ggcggtctaa ttgatgtttt cagaaacaga
     2341 ctcatgttcc cgataattga tttaaagggc aatgtaatag ccttcggcgg cagagttctc
     2401 ggcgatgaaa agcccaagta cataaatacc tcggacactc ccgtttacaa aaagggcaag
     2461 ggcattttcg ccctgaatat cgcgaaggcc cacggcagaa gtttaatcct ctgcgaaggc
     2521 tatatggatg tcatcgccat gcatcaggca ggctttcaaa acgccgtggc gggccttggc
     2581 accgccttta cctcagagca ggcgcagatt ctctcaaggt acgccgacga ggtaattctc
     2641 gcctacgaca gcgacgacgc gggaaaaaaa gccgcaaagc gcaccatgtc gattttccag
     2701 tcgatgaatg taaaaaccaa aatcctcacc ttaacaggcg gcaaagaccc ggacgagatt
     2761 ataaaacgct ttggcaggga gtatatgcaa aaaatcatcg acgggtcctt aagctccaca
     2821 gaatacaagc tcgccctagc caaagagggt attaacaccg ataccgcgga tggtaaagtg
     2881 gaatatctaa aaaaggcggt cgaagtcctc tctgagcttg acgacgccat tgagcgggat
     2941 gtatatgcgg gcagactctc tgaagaaacg ggagttaccc gaaccgccat agacgagcag
     3001 ataaaaagag ctctcagctt cagaaagaga aagcaggaca tcgatagttt caataaagca
     3061 agggagattt tagagggcaa ggtaatcgca gcaaaaaacg ccctcaagct ctacaatcaa
     3121 agcgcgagtc ttagatgcag gcaagctgaa gaaactctcc ttgcctcgct tattcaaaat
     3181 cctgagtttt ataaaaagct taaggataaa ataacgatag acgatttttc gtctgctttg
     3241 cataaaagat tatttttcaa aatcaccgag agaatcgaaa acaatcagga gcttgatctc
     3301 atctattttg cggatgagtt taatcccgaa gaaatgtcga ttctcacaaa ggtttttcaa
     3361 aaaggcagaa ccatttccaa tactttggac gagtgccttg attgcataaa ggtaatgagc
     3421 gaagaaaaac taaagcagag cgctgccgat ccgagcaagc tctctgacga agagtttctg
     3481 agattatttt acaaaaagga ataatactaa acaccgggac aataatttgt gtgaagggga
     3541 tattgatgga caacaacgaa aagcgcgcgt ttgtgaacat gcttatcgac aagggtaagg
     3601 ctgaaggcag gcttaccgct caggagatag acgcggctat cgtggaaatg gatttcgata
     3661 ttgatgagct ggacaagctc tacgaaacaa tcgagcagaa caatatcgaa atcatcgacg
     3721 atatgtccga cgctgctctt gacccgtctc tttttgatac ggaagccgta aagtctgccg
     3781 acgcaagcgg cgacttaaag ggtcttgcga tggacgaccc tgtcaaggtc tatctcaagg
     3841 agataggcaa ggtgccgctg cttacgcccg aggaagaaat cgagctggca atcagaattg
     3901 ccaacaacga ccaggaggca aaaaaacgcc tccaggaagc aaacctcaga cttgtggtaa
     3961 gcatagcaaa aaggtatagc ggaagaggta tgcagttcct ggatttaatt caggaaggca
     4021 acctgggact catcaaagcg gtcgaaaagt tcgactacac aaagggtttc aagttctcca
     4081 catacgccac atggtggatt cgtcaggcaa tcaccagagc cattgccgac caggcaagaa
     4141 caatacgaat ccccgtccac atggtggaga caatcaacaa ggtcaaaaag accaacagcc
     4201 agctgctgca taagaacggc agagacccga ccgctgaaga aattgcggaa gaacttaata
     4261 tgcccataga aaaggtaagg gaaattctca cctttgccca ggagcccgtt tcccttgaaa
     4321 cgcccatagg cgaggaggag gacagccatc tcggcgactt cattcccgac gacgacgccc
     4381 ttgcccccgc agacgcagcc tctcagcttc ttttaaagga ggctcttgtc tccgtgctca
     4441 aaaccctcac tcccagagag gcaaaggttc tgtctcttcg tttcggactt gaggacggac
     4501 actccagaac ccttgaggag gtcggcaagg agttcaatgt cacaagggaa agaatcaggc
     4561 agattgaggc aaaggcttta agaaagcttc gccatccgca aagaagcaaa aggttaagag
     4621 atttcttgga ctaagccgat tgcgcatctg gctatacata tctattaaaa taagtggggt
     4681 atcggcaaga ttccccgctt aagtttatat gaaggtttag gggtttaaat gataagactt
     4741 aatgagctca agctgcccat cggagcaagc gaggaagatg taaagagcct tgccgcaaag
     4801 gagcttaata ttcccgtctc ggaactgaag gacttcagaa tagcgagaat gtcaatagac
     4861 tcaaggaata aagagaggat tagacttacc ttttccgcag agtttacggt ggacggggat
     4921 gaaagctccc ttatttcaga ttttcctcca aacagggcaa gcgtttttga gccttatgaa
     4981 tacacgcctc ccgcagtaaa cagaacatcg cctttaagac cggtcatcgt aggcttcgga
     5041 cccgcgggaa tgttcgccgc cctccttctt tcaaaggcgg gcttaaagcc cattgtcctt
     5101 gaaagaggcg aaagggtgga agacagaacg gaaacggtaa agcgcttttg gaagaacaga
     5161 gctttaaaca ccgactccaa tgtccagttc ggcgagggcg gagcgggcac attctccgac
     5221 ggcaagctga caacgggcat taaagacccg agagtccgct atgtgctaag cacccttgcc
     5281 gagcacggcg ccccaaagga gattctctat tcagcaaaac cccatatagg aacagacctt
     5341 cttgcgaatg ttgtcagaga tattagagaa accgtaataa aaaacggcgg tgaaatccgc
     5401 ttcggctgcc gccttaccga tttaatcatc tcaaacggcg ccgttttcgg agtccgctac
     5461 cttgaaaacg gaaaagccga aagagaaata gaaaccgact gcgttctcct ttgcataggt
     5521 cattccgcaa gggatacctt cgagatgctc ctcggaaagg gcgttaaaat gcaaaaaaag
     5581 cccttctccg tgggcgtgag aatagagcac ccgcaggagc ttatcaacaa aatgcagtac
     5641 ggcagggagt ggaacaaccc gcttttggga gccgccgact ataagctgtc aaaccacccc
     5701 aagcacggca ggggaggcta taccttctgc atgtgccccg gcggagtggt tgtcactgcc
     5761 tcctcagagg agggtatgct ggtggtcaac ggcatgagcg aatacgcccg cgacggagag
     5821 aacgctaact ccgctatcct tattggcgtt gagcccgatt actttgaaag cgagcacccg
     5881 ctgagcggca tcgaatttca aagagagctt gagagaaaag ccttcctcct tggcggcgga
     5941 gattataccg cccccgctca gcttgttggc gactttataa aggacagacc ctctgcaaag
     6001 ctcggcgctg taaaaccctc ctgcacaacg ggggtcacga tgtcggacat aagaaaagtt
     6061 ctgcccgata acatcaccgc gccccttaaa gacgcccttt tctcctttga cagacagttc
     6121 aagggatttc tccttcccga cgctgtcttg acggcggttg aaagcagaag ctcctcgccc
     6181 gtcagaatcc tgcgtgacga tttgtatcaa tcaaatataa gaggtcttta cccctgcggt
     6241 gagggagcgg gctatgcggg cggaattgtt tccgccgcgg ttgacggtct cagatgcgcc
     6301 gaggcggttt taaaggacga gacataaaaa aagaggagct ttaagctcct ctccgaagtt
     6361 atcagtcaac ggggtatttc acgccgacct gtcttcttat ctcgtccatc agcctcataa
     6421 tgagcctgct ctggtcgtgg gtcataatgt cgctttcctt cttgccttga cgcaggcact
     6481 ccgaagcctc cataatctgc tcctcaaagc cgttgccgta gtaggggcag tcaatgactt
     6541 caacatcctt gccctctatg tgcagctcta tcttctgcgg agcgtatgac ctttccacat
     6601 aagcatagcc cttttctccg aacaggtagc cttcattggg ctttctgagt gtcatacccg
     6661 atgacagcga ggcaatcgcg ccgtttttat atttgataag atagcagcag tggagatcca
     6721 cgcccttgtc gctcagctca gcgacggcgg taatctgctc aggctccgaa tttgcgtacc
     6781 aagatgcgaa gctcaggcaa tagcagccca catccaaaat agagccgccg ccgtatttcg
     6841 ggtcaaaggc gtgatggtcc ggctcatcac tcatatcata gctgaaggtt gactgaatgc
     6901 ctctgatttt tccgagagag cccgactcca caatttcaag gagcttcttt gttccgggca
     6961 caaaccttgc ccacattgcc tccataagga aaacatcgtt ctcctttgca gaggcaatca
     7021 tgcgctccaa ctcagacata ttaagggtta tcggcttttc tgacaaaacc gcttttttat
     7081 ggttcataaa aagtatggcg ttatctgcgt gaagtccgtg gggagtcgct atgtaggcta
     7141 tgtcaatgcc gtcaaaagcg gccatttcct catagctgcc aaaagcgtga ggaatattgt
     7201 acttcttagc aaattgttcg gcctttgcaa tatcgcggga cgctaccgcg caaagctctg
     7261 cgccgggtgt gttgttgcag gcctccgcaa atctgtttgc gatattgccc gcgcccacaa
     7321 tgccccatct tatagttttc attgtcagcc ctcctaatta aagctgataa aatcatatca
     7381 acaacagggt aattatgtca accgtaaaag gagtttctat gaaaacagga gttattgata
     7441 tcggcggcgg aatgagagga atctatgccg cgggtgtctt tgacgcgttt attgattttg
     7501 gtattaaatt tgactactgt atcggcgttt ccgcgggtac ggcaaattta gtgtcctttc
     7561 ttgcgggtca aaaaggcagg aattacagat tctatgtccg ccacgctcag gacaaaaggt
     7621 atatgagcct tgagaacatg attaaaaagg gcgagtattt tgacctttct tatatatacg
     7681 acactcttac aaaccatgtt gacccacttg actatgacgc cctgataaac tccccgagcg
     7741 agcttaaaat agtcgcaaca aacgcaaaaa ccgcccttcc cgagtatttt gacaaagagg
     7801 tttttttaga aagaagctgc aaggctctta tggcgtcatg cgctttgccc atagcctgca
     7861 gagcggtgaa aattaacgat gtggagtatt ttgacggcgg agtctccgac ccgattccaa
     7921 taagaaaagc aatcgaggac ggctgcgaca gggttgtggt gctgctcccg agaaatgtga
     7981 atctcagaaa aaagcccgag agcctgagat atgtctatct cagagccctc agaaaatatc
     8041 ccgagataat cgaagctata aaccaaaggc atgaaaaata caacagggcg attgacgaac
     8101 ttattgaact cagagaacag ggcaaggctg ttattatctc ccccgcggta caaataaacg
     8161 cgaggataaa ctcaaagaac ccggcattgc ttgacagact gtataagcag ggcaggcgtg
     8221 acgcggttaa agttttgccc gaaattagaa aatggggttt ataaaatata aaaagggagc
     8281 ggcaatcgct cccttctatt ttttatttgg ttagtccaga actacctcat gtcccagctc
     8341 tgccgacttg taaaccgagt caagtattct cattatgtca acgccgtcct gagcgggcgc
     8401 aaggcagggt gttccgttaa gtatgcagtc gacaaaatgg ttaatctcgt ttgcgaaaag
     8461 acccttaaag ctgagagccg tgtcatgcgg gaactcgata ttaaccatgt agtcgttaag
     8521 ctcggtataa agctcaacct gaggcttaat aatagctccg ccctttgtgc cgaacagctc
     8581 aatgtcgccc ttgccttctt taaggttcag gctgtagctt gtctcaaggg atacaacagc
     8641 gccgttgtca aagcggataa gagctgtcgc aaggtcctca acatcgcaga tgtcgttatt
     8701 gcctgcctca gcagatgtat agcccttctt gcccttaatg tggggacggt tgaacagctt
     8761 ctggaatgtg gcgccgtaaa ccgaaatcgg cttaggattg cccattaagt atcttacaag
     8821 gtcaattaca tgaacgccaa ggtcaatcag cgggccgccg ccggaacggg acttatcgcc
     8881 gaaccagccg ccggggttgc cgtttctgcg cagatattga gcctttgcgt agtagatttc
     8941 gccaaggtaa cctttgtcga tataatcctt taaaagagcg cagtcattgc cgtgacggcg
     9001 gacaaagcct atcatcaaaa gctttcctgc cttgtcggcg gctgccttca tttcctttgc
     9061 ctcttcctca ttcatagcca tcggcttttc gcacagaaca tgcttgccgg cattgagagc
     9121 ggcaatggag cactcagcat gagccgagtt ccatgtgcag acgctgacag cgtcaatttc
     9181 aggcagagcc ttgaacattt cgtccttgtc ggtaaatgtt ctctccagcg gcacattgta
     9241 ctccgcggct cttttttcaa cgcgctcctt gtttatatcg cagagagcgt agatttctac
     9301 attgggattc tccttatagg catcaaggtg ctcacggctg attgagcctg tacctataac
     9361 agcaatttta agtttctttg acaaaaaggc ccactccatt ctatttttcg tagcattttg
     9421 ctttatataa taatagcact aaaccgcaaa ttttcaatag cttataatca aaagacaata
     9481 aaaaagcagg gagttttcct ccctgctttg acccttattt acttgcagaa aacatgcgag
     9541 ccaattgtaa cggtgacggg tctttttctt atccactggt tggaggtttt tgcgggatta
     9601 taatagtata tagctccgcc tgtggggtcc catccgttta cggcgtcctg agccgccttt
     9661 ttagctgtgg cgttgggggc tacgctccag ttgctgtcgt aaacgcagct gaatgcgccc
     9721 ggctgataaa caacgccgcc aattgtgtcg gggaatgagg ggtgccttac tctgttgata
     9781 ataaccgcgc ctaccgcaac ctgaccagta taagattcgc ctcttgcctc agcgccgatg
     9841 actcttgcca gaaggtacat atcgctggag ctgaattgtc cgccgcctcc gccaccgaga
     9901 ccgagataca acagagtctg cggacctgcg attccgtcaa ccttgatgcc gactgcgcgc
     9961 tggaacgcct taaccgcgtt ctgagtcata actccgtaaa ttccgtcaac attaccttta
    10021 tagtaaccga gctctttgag ctttttctga attctcctta cctcatcgcc ccttgagccg
    10081 agcttggata aggtatcatc agccgcaatc tcttcaatcg agccgtcata tatagccgta
    10141 tagctgtcgg aaacggcgaa aattccgtaa gcgacagaga atataagagc agtcaccgtt
    10201 acaaagtaaa atgcctttga aatcggactg acttttttgc tggatttatt cttaggtttc
    10261 atctcgattc cctcacaatg atgaatttca aatacagttt atccactatg agcaaatttt
    10321 ttcaaagaag cataatataa aatttgccaa gagcggtttt attcaatttc tcgacagaaa
    10381 aatctttaat attctttcaa tattagtctt ttttaacgca taaaaactgc cttaaagtca
    10441 caaaatgtta ttagtggccg aagtataaga aagattttaa aaaaatcaaa aaaaattcta
    10501 aaaaaggagt tgacaatatc aaaaaagcta tgctattata cttgagccgt ccggaagggc
    10561 ggtaaacgag cgaagagctc gggcgattga aaaagccaaa aaaaggcaaa aaaatagctc
    10621 ttgacaagaa aagcgagata tggtaaatta aaagtcccgc gatacggcag gaagctgtcg
    10681 tagggttgga aagagttacg gaccttgaaa attaaacaac gacgaaagca aagaaaccct
    10741 gagatttctt tgagtaagtg aagaacttac gaagtaattc cgaaaaggaa taaatgagct
    10801 ttagaacgct ctaagatagg ttataccggc attagccgat gtaataccat atttttagag
    10861 agtttgatcc tggctcagga cgaacgctgg cggcgtgcct aacacatgca agtcgtacgg
    10921 agttcacttg gaagcttgct tccgagtgaa cttagtggcg gacgggtgag taacgcgtga
    10981 gcaacctgcc tcacagaggg ggataacaca cggaaacgtg tgctaatacc gcataatccg
    11041 atgaagcggc atcgctttat cggcaaagga gcaatccgct gtgagatggg ctcgcgtccg
    11101 attagatagt tggtgaggta acggctcacc aagtctacga tcggtagccg gactgagagg
    11161 ttggacggcc acattgggac tgagacacgg cccaaactcc tacgggaggc agcagtgagg
    11221 gatattgcac aatgggcgaa agcctgatgc agcgacgccg cgtgagggat gacggtcttc
    11281 ggattgtaaa cctctgtcct tggtgaagaa gaaagtgacg gtagccaagg aggaagccac
    11341 ggctaactac gtgccagcag ccgcggtaat acgtaggtgg caagcgttgt ccggaattac
    11401 tgggtgtaaa gggagcgtag gcgggcttgc aagtcagctg tgaaattcaa gggcttaacc
    11461 cttgacatgc agttgaaact acaggtcttg agtgaagtag aggtaggcgg aattccgagt
    11521 gtagcggtaa aatgcgtaga tattcggagg aacaccggtg gcgaaggcgg cctactgggc
    11581 ttttactgac gctgaggctc gaaagtgtgg ggagcaaaca ggattagata ccctggtagt
    11641 ccacaccgta aacgatgatt gctaggtgtg gggggtctga ccccctccgt gccgcagtta
    11701 acgcaataag caatccacct ggggagtacg gccgcaaggc taaaactcaa aggaattgac
    11761 gggggcccgc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn ntgagatgtt gggttaagtc ccgcaacgag cgcaaccctt gtcattagtt
    11941 gctacggaca gagcactcta atgagactgc cgttgacaaa acggaggaag gtggggatga
    12001 cgtcaaatca tcatgcccc
//
LOCUS       DAONVQ010000057        11990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4097_length_11990_cov_4.708840, whole genome shotgun sequence.
ACCESSION   DAONVQ010000057 DAONVQ010000000
VERSION     DAONVQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4097_length_11990_cov_4.708840"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLD98_04970"
     CDS             complement(<1..180)
                     /locus_tag="PLD98_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05634.1"
                     /translation="MRRILSINIIDVVPFSKGLLFVRKEQLSNGSVKVSFFSYDITTG
                     KIAPVTKSVYLLNKFG"
     gene            complement(216..872)
                     /locus_tag="PLD98_04975"
     CDS             complement(216..872)
                     /locus_tag="PLD98_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HQA05635.1"
                     /translation="MKDSTGISMSVIRRLPRYYQYLTDLSNNGIERISSSELAEKMGL
                     TASQIRQDFNCFGGFGLQGYGYNTKQLANALAGIMGLDSDFSAILIGAGNLGRAVAAH
                     LFSEQNGFKLIAVFDSDKKLEGKKVGKFSVMHMDALEDFCRENKPVTAVLCVPDEAAK
                     EVALKLDSLGVKSFWNFSHFDISSILPNSRVENVHLKDSLMTLCFLIKSDMEKNKEKH
                     "
     gene            complement(906..2081)
                     /locus_tag="PLD98_04980"
     CDS             complement(906..2081)
                     /locus_tag="PLD98_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HQA05636.1"
                     /translation="MNEANIKSAAEKFEALLREQLERAEKIKEVKDFIDYSTLDTIVI
                     GVCYGDGIGPIITKEAARVLEHLLKKEVESGKVLFKVIDGLTIENRIKVNKAIPDDVL
                     EELKSCHVILKGPTTTPQAGDGMPNIESANVAMRKALDLFANVRPVKVPEQGIDWTFF
                     RENTEGAYAVGSKGINVSDDLAVDFVVTTTEGTERIARLAYEYARKNGKDRVSIVTKA
                     NVIKTTDGKFLNLAKEIGKEYPEITTDEWYIDIMTAKLIDEKRRRDFKVFILPNLYGD
                     IITDEAAEFQGGVGTAGSANIGKRYAMFEAIHGSAPRMIKEGRGDYADPCSMLRASVM
                     LLSHIGMQDKADLLERALDICMFEEKKVVITGRPGGGTCREFGDYLMSVLDRLSSEQ"
     gene            complement(2097..4025)
                     /locus_tag="PLD98_04985"
     CDS             complement(2097..4025)
                     /locus_tag="PLD98_04985"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HQA05637.1"
                     /translation="MGLSITHKLISSHLVSGKMVPGEEIGLKIDQTLTQDATGTMAYL
                     QFEAMGIDRVKTELSVSYVDHNTLQSGFENADDHKFLQTVAKKRGIYFSRPGNGICHQ
                     VHLERFGVPGKTLLGSDSHTPTGGGIGMLAMGAGGLDVAVAMGGGTYYITMPKVVNVK
                     LIGKLSPWVAAKDVILEVLRRLTVKGGVGKVIEYTGEGVKTLSVPERATITNMGAELG
                     ATTSVFPSDEITREFLKAQGREKDWVEILPDEDAVYDEEIVIDLSELKPLAAMPHMPD
                     KVKAVSEIGPIKIDQVCIGSCTNSSLLDMLKVAAILKGKTVHPDVSLTISPGSKQVLN
                     MLSDLGALSDIIAAGARILESACGPCIGMGQAPKSGGVSLRTFNRNFEGRSGTADAQV
                     YLVSPETAAISAITGVLTDPQTLGEMPEFTLPSMFKIDDNMIIPPAPAEEADKVEIVY
                     GPNIKEVPKGRPLEDELKATVVLKVEDNITTDHIMPAGAKILPYRSNIPYLSQFCFKQ
                     LDENFAENCKKVGNAIVVGGQNYGQGSSREHAALVPLYLGVRAVIAKSFARMHKANLI
                     NAGILPLTFINEADYDRIEKDDVLTMPYVKDEVLNGKDITLKNETKGETYKLAGGFSD
                     RQKDILISGGLLRYTANK"
     gene            4252..6582
                     /locus_tag="PLD98_04990"
     CDS             4252..6582
                     /locus_tag="PLD98_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05638.1"
                     /translation="MLDIMFLILGSLSAIVFSLYTFFNLSEKSRAKKLKPLAFGALGL
                     SFLLTLSEITGIAKLYHIKCFNAWVFAVSAVVFVLLEFSGKFIKTEKGTAHLAFFAKT
                     LFVALFFELFVFSFNSYKLMPKDYPHINADLSEAALDGEIIGDKLYIKGSRFETSAII
                     KLKNIGVPVGTLTVDLESDTNQKTKVNISYSDETNQKLRHNIAAGEIINGNEKSKTFP
                     INASGKVGDILIEIPVSEGERIVVNSVEVNKPMGLKASPVRFLIVVLGALLIYLLLNA
                     ELMKKPFYKNEFAAKVLVFAVTAVFVLYAFFLIYGKTGDIMTEFRTTKGNQITQELVD
                     AFKKGQVHLIEEPSKELLELENPYDKSLRQDMDILWDHVLYNGKHYSYYGIAPVLFLF
                     LPYNLITGYYFSTPWAVLLFGCLGMIFLSLLYYEFIKKFHKDTPLNLFIGSLVLLQMA
                     SGVLYCFPTPLFYEIAQTSGFLCITAGAYFMLKSNVIGEGEIRLPSLCLSSTFLALAV
                     LCRPTLAVYSVVALLFIFAGFNKLRGKVGNKAYKDFIKYAAASLIPFAFFGGIQMIYN
                     YLRFDSPLDFGIQYSLTINDFTRAEFHVHLAAIGFFNFLFAFPEFSPDFPFFVSRVQT
                     FSPNGYYFVATNTAIGLIFKAMPVLSLGYWRKAYKNSDSKNKKLYTALLFALGIAAPF
                     IIIASIWESGYGVRYGADFNWQILVCAFAIMFTIYNRLQNKGVKKILTQLFVFSVVVS
                     VILVTTELWSWQVYNKPSDEILRLLFSFGRIFEFWK"
     gene            6783..7124
                     /gene="rplS"
                     /locus_tag="PLD98_04995"
     CDS             6783..7124
                     /gene="rplS"
                     /locus_tag="PLD98_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQA05639.1"
                     /translation="MDALKIIEAGQLKENVPEFAVGDTVRVHVKIREGERERIQAFEG
                     TVIARKGSGVSETFTVRRVSYGVGVERVFPLHSPNVANVEVVRRGRVRRAKLYYLRDR
                     VGKAAKIKEQI"
     gene            7242..7844
                     /gene="lepB"
                     /locus_tag="PLD98_05000"
     CDS             7242..7844
                     /gene="lepB"
                     /locus_tag="PLD98_05000"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQA05640.1"
                     /translation="MRRREDYIIEFDYEESEPQQKPPGRFWLYVYDFFGSISGALFTV
                     FIVFTFLFRMVDVDGDSMLNTLSDGDWLCISSLYFEPEYGDIVIISRENTNEPPIVKR
                     IIGKENDVIDIDFHNNTVSVNGKILDEPYVSTPTQRSFDVSFPLTVPENCVFVLGDNR
                     NHSLDSRSSLVGCVDENRILGKVLVRLTPEITFFIGQKGH"
     gene            7848..8381
                     /gene="lepB"
                     /locus_tag="PLD98_05005"
     CDS             7848..8381
                     /gene="lepB"
                     /locus_tag="PLD98_05005"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQA05641.1"
                     /translation="MKDKIVDMAYEVASVLVSSIIIIAIIFTFAFRLVGVSGHSMDTT
                     LSHGDWLLVTTTYTEPKYGDIVVIAQPTYFKEPLIKRVIAVGGQTVDIDNETGTVYVD
                     SKALDEPYISTPTTNPTVSQIDFPVVVPYGYLFCMGDNRNNSTDSRSSLVGFIDERYI
                     LGKALKRVVPFGKWNIY"
     gene            complement(8468..9394)
                     /gene="tsf"
                     /locus_tag="PLD98_05010"
     CDS             complement(8468..9394)
                     /gene="tsf"
                     /locus_tag="PLD98_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQA05642.1"
                     /translation="MSFTAKDVQALREKTGVGMMECKKALVEANGDMDKAIDILREKG
                     LAAANKKAGRIAAEGVVLAYRDNDLGVSVLVEVNSETDFVAKNEEFRAFVLDVAKTIA
                     KTNPADVETLLATNLVGSDRTVQEHVQDKVLSIGENIKVRRFVRVEGNTVEYIHGGGT
                     VGVIVVLEADKETAAKEEVIAMGKDVAMQIAAMSPLYLKESDVPADVIEHEKGILMAQ
                     IKADEKMANKPEKVIEGIVKGKLSKYFKETCLLEQQFVKDEDITVAQYIESVAKQVGA
                     PVAVSAFYRYAKGEGIQKKEDNFAEEVSSMIK"
     gene            complement(9516..10304)
                     /gene="rpsB"
                     /locus_tag="PLD98_05015"
     CDS             complement(9516..10304)
                     /gene="rpsB"
                     /locus_tag="PLD98_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQA05643.1"
                     /translation="MAVVSMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKLEEAYAFVREIAANGEEILFVGTKKQAQESIKEEAERCNMPYVNARWLGGMLT
                     NFNTIKLRIKRLAQLKAMEENGTFDLLPKKEVVKLKLQIEKLEKFLGGITEMRRMPAA
                     MFVVDPRKEKIAVAEARKLGIPIIAIVDTNCDPDLIDYVIPGNDDAIRAVKLIAGAIA
                     DAVLEGRQGEQFAPAAEGDENAEEAAEAVEAAEAEEAVEAADAE"
     gene            complement(10508..10984)
                     /locus_tag="PLD98_05020"
     CDS             complement(10508..10984)
                     /locus_tag="PLD98_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004442492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQA05644.1"
                     /translation="MVKYEDKVVVTLDINGEVHKAAVRPSDVLLDVLRQKLGLTAAKP
                     GCKNGDCGTCTIIIDGLPAKSCLVLAVEAENHKIQTVEGLRGQSPAQRAFVDANAFQC
                     GYCTSGFLMVCEALRQNYPTYPDEHIVEEWLQSNLCRCTSYKEIRNAVRLMYEYKT"
     gene            complement(10977..11807)
                     /locus_tag="PLD98_05025"
     CDS             complement(10977..11807)
                     /locus_tag="PLD98_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQA05645.1"
                     /translation="MIPFNFTYYRPDSLEEAVRIHSELTSKGLSAVYYSGGSEIITMC
                     RAGSIRPDAVIDIKEIPELKSLAMDESCLRIGSACTLYKIKQSKAYPLLALACGRIAD
                     HTNQCRITLGGNICGTIIYKETVPPLLLSDAEVTLLGPEGKRTVPFSDVFDGRIHLLP
                     SELLVSAQIPKWALSAQHFHIKLTAQEKIDYPLVSIAALIKDNFLRIAFSGVCSFPFR
                     SQQIELVLNNKSLSISERAERAASLIPEEYSDRAGSAEYRRFVFKNAIEELLEEWENG
                     "
BASE COUNT         2975 a         2889 c         2676 g         3450 t
ORIGIN      
        1 gccgaattta ttaagaagat acacgctttt tgtaacgggg gcaattttgc ccgtggtaat
       61 gtcgtagctg aaaaaggaca ccttgacaga gccgtttgag agctgttctt tgcgcacaaa
      121 aagtagcccc tttgaaaaag gaactacatc gattatatta atgctcagaa ttcttctcat
      181 cgaacatatc ctttctgata gcgctgaagg aagaatcagt gcttttcctt attcttttcc
      241 atgtcggact tgataaggaa gcaaagggtc atcaggctgt ctttaaggtg tacattctca
      301 acccttgagt tgggaagtat cgacgagatg tcaaagtggc tgaagttcca gaagctctta
      361 actccgagag agtcgagttt aagagctact tctttagccg cctcgtcggg aacgcaaagc
      421 accgcggtaa cgggcttatt ctccctgcag aaatcctcaa gggcgtccat gtgcattact
      481 gagaatttac cgaccttttt gccttcaagt tttttatccg agtcaaaaac cgcaatgagt
      541 ttaaagccat tctgctcgga aaacaggtgc gcggcaacgg ctctgcccaa attgcccgcg
      601 ccaatcagaa tagccgaaaa atcagaatca agccccatta taccggccaa agcgttcgca
      661 agctgctttg tgttgtagcc atagccctga agcccaaatc ctccgaagca gttaaagtcc
      721 tgccttatct gtgacgcggt aaggcccatt ttttctgcca gctcgctgga ggaaattctt
      781 tctatcccgt tattactaag gtcggtaaga tattgataat agcgggggag ccttctgatt
      841 acagacattg aaattccggt tgaatccttc atatgctcct cctgatttta tgataatgta
      901 gaaaattact gttcgctgct gagtctgtca agaacagaca taagataatc gccgaactcc
      961 ctgcaggttc cgccgccggg tcggcctgtg ataacaacct ttttctcctc aaacatgcag
     1021 atatcaagag ctctttcaag caaatcggct ttgtcctgca tgccgatgtg tgaaagcagc
     1081 attacggagg ccctcagcat ggagcagggg tctgcgtaat ctcctctgcc ttccttaatc
     1141 attctgggag cggagccgtg gatagcctca aacatggcgt atctcttgcc tatgttcgcg
     1201 ctgccggctg tgcctacgcc gccctggaac tccgcggcct catcggtgat gatgtcgccg
     1261 taaaggttgg gtaggataaa taccttaaag tcccttctgc gcttctcatc aataagcttt
     1321 gcagtcatta tgtcaatata ccactcgtct gtggtaattt cgggatattc cttgccgatt
     1381 tcttttgcaa ggtttaagaa tttaccgtct gttgtcttta tgacattagc ctttgtgacg
     1441 atagaaactc tgtccttgcc gttctttctt gcgtactcat aggcaagcct tgcgattctc
     1501 tcggtaccct cggttgtggt aacaacaaag tcaaccgcca aatcgtcgct tacatttatg
     1561 ccctttgagc cgacggcgta tgcgccctcg gtgttctctc tgaagaatgt ccagtcaatg
     1621 ccctgctcgg gaaccttgac ggggcggaca ttggcgaaaa ggtcaagagc ctttctcatg
     1681 gcgacattcg cgctctcgat attcggcatg ccgtcgcctg cctgtggggt ggttgtgggt
     1741 cccttgagaa ttacatggca ggattttaat tcctcaagca catcatcggg aatagctttg
     1801 ttgaccttaa ttctgttctc tatggtaaga ccgtcaatga ctttaaacag aaccttgccg
     1861 ctttctacct cttttttgag caggtgctca aggacccttg cggcctcttt tgtgatgatg
     1921 ggaccaatgc cgtcgccgta acaaactccg attacaatgg tgtcaagggt agagtagtcg
     1981 ataaagtcct taacctcttt tattttctcg gctctctcga gctgctctct caaaagagcc
     2041 tcaaattttt cagcagcact ctttatatta gcttcgttca tatttacact cctgatttac
     2101 ttgtttgcgg tgtatcttaa aagcccgccg cttataagga tgtccttctg cctgtcggag
     2161 aatccgccgg caagcttata tgtctcgccc ttagtttcgt ttttaagggt aatatctttg
     2221 ccgtttaaga cctcgtcctt aacatatggc attgtgagga catcgtcttt ttctattctg
     2281 tcatagtctg cctcgtttat aaatgtaagg ggcagaattc ccgcgttaat aaggtttgcc
     2341 ttgtgcatgc gggcgaagga ttttgctata acagccctga ctccgagata gaggggaacc
     2401 aaagcggcgt gctctcttga tgagccctga ccgtagttct ggccgcccac aacgattgcg
     2461 ttgccgacct tcttgcagtt ttctgcgaaa ttctcatcaa gctgcttgaa gcagaactga
     2521 gagagatagg ggatattgga gcggtaggga agtatctttg cgcccgcggg cataatgtgg
     2581 tctgtggtga tattgtcctc taccttgagt accacagtag ccttgagttc gtcctcaagc
     2641 ggtctgccct tgggaacttc cttgatattg ggaccgtaaa ctatctcaac cttgtccgcc
     2701 tcctcagcgg gagcgggagg tataatcata ttgtcatcaa ttttaaacat cgaggggaga
     2761 gtaaactcgg gcatttctcc gagtgtctgt gggtctgtaa gaacgcccgt aatcgctgaa
     2821 atagcggcgg tttcggggct gacgaggtaa acctgtgcgt cagcggtgcc cgaacggcct
     2881 tcaaagtttc tgttaaatgt cctgagcgaa acaccgcccg atttgggtgc ctgtcccatg
     2941 ccgatgcagg gtccgcaagc gctctcaaga attcttgcgc ccgcggcgat aatatccgat
     3001 aaagctccca aatcgctgag catattaaga acctgcttgg agccggggga tatggtaagg
     3061 cttacatcgg gatgaaccgt cttgcccttt aaaatcgccg cgactttaag catatcgagg
     3121 agagaggagt ttgtgcatga accgatgcat acctggtcaa tcttaatggg accaatctcg
     3181 cttacagcct ttaccttgtc gggcatatgg ggcattgctg caaggggctt gagttcggac
     3241 aaatctataa cgatttcctc atcataaaca gcgtcctcat cgggcagaat ctccacccaa
     3301 tccttttcgc gaccctgagc ctttaagaat tcccttgtaa tctcatcaga ggggaatacg
     3361 cttgtggtgg cgcccagctc tgcgcccatg tttgtgattg tagccctttc gggaacggag
     3421 agggtcttta caccctcgcc tgtgtactca ataactttgc cgactccgcc ttttacggtg
     3481 agtctcctta aaacctcaag aataacatcc tttgcggcaa cccacggtga gagcttgcct
     3541 atgagcttta cattaacaac cttgggcatg gttatgtagt aagtgcctcc gcccattgcg
     3601 acagcaacat caagaccgcc agcgcccatt gcgagcattc cgatgccgcc gccggtgggg
     3661 gtatggctgt cggagcctaa gagagtcttt ccgggtacgc cgaaacgctc aaggtgaacc
     3721 tgatggcaaa tgccgttgcc gggccttgag aaatatatgc ctctcttttt tgctactgtt
     3781 tgcagaaatt tatggtcatc ggcgttttca aagccgctct gcagtgtgtt atggtcaaca
     3841 taagaaacag aaagctctgt tttcactctg tcaatgccca tagcttcaaa ctgaagatag
     3901 gccattgtgc ctgtagcgtc ctgagtaaga gtctggtcga ttttaagacc gatttcctca
     3961 cccggaacca tttttccgct gaccaaatgg gatgaaatca gcttatgtgt aattgataaa
     4021 cccacaaata tcaccttttt tcacttcatt ctgtcttatt ttacttgctt gtgactttat
     4081 tgtcaataac actgaccgag atagcggata atattaatta attcttaaaa aagactcagg
     4141 cagtatttct aataggaaaa attatgctat aattgcatca gtttatattg ttttattatc
     4201 tgatattagg taaactaatc gggttacagc acaaagcaaa ggagctttaa aatgcttgac
     4261 ataatgtttt tgattttggg aagtctttca gcaattgttt tcagcctata tacattcttt
     4321 aatctctccg aaaagagccg cgcgaaaaag ctgaaaccct tagcctttgg agctttggga
     4381 ctttcgttct tgttgacgtt atccgaaata acgggaatcg caaaactcta tcatataaaa
     4441 tgtttcaatg cttgggtatt tgcggtttcg gctgttgtat ttgttttgct tgagttttca
     4501 ggaaaattca taaaaacaga aaaaggcaca gcacatcttg ccttctttgc caaaacgctc
     4561 tttgtcgctt tattctttga gctttttgtt ttcagcttta attcctataa gctgatgccg
     4621 aaggattatc cccacataaa tgcggattta tctgaggccg cattggacgg cgaaattatc
     4681 ggcgacaaac tttacataaa aggaagcaga tttgaaactt ccgccattat aaagcttaaa
     4741 aacataggtg ttcccgtcgg cactctcacc gtcgatttgg agtccgacac gaatcaaaag
     4801 actaaagtaa atataagcta ctccgatgaa accaaccaga aactaaggca taacatagcc
     4861 gcgggggaga ttataaacgg taatgagaaa agcaaaacct tccccataaa cgcctctgga
     4921 aaagtagggg atatccttat agaaatcccc gtttcagagg gagagcggat agtcgtaaac
     4981 tccgtagaag tcaataagcc catggggctt aaagcgtccc ccgttaggtt cttgattgtg
     5041 gtactcggcg cattattaat ttatctcctt cttaacgctg agcttatgaa aaagcccttt
     5101 tataaaaacg aattcgccgc aaaggtgctt gtctttgccg ttaccgcggt gtttgtcctt
     5161 tacgccttct tcctgattta cggcaaaacg ggcgacatta tgaccgaatt cagaaccaca
     5221 aagggcaacc agattactca ggagcttgtt gacgccttca aaaaaggtca ggttcacttg
     5281 atagaggagc cttcaaagga gcttttggag ctggaaaacc cctatgataa gagcctaaga
     5341 caggatatgg acatactctg ggaccatgtt ctttataacg gaaaacacta ttcctactac
     5401 ggcatcgccc ctgtattgtt ccttttcctc ccgtataacc tgattacggg ctattatttc
     5461 tcaacaccgt gggcggtact gcttttcggc tgcctcggca tgattttctt atccctcctt
     5521 tactatgagt ttataaagaa gttccacaag gatacgccgt tgaacctctt tatcggctct
     5581 cttgtgcttt tgcagatggc aagcggagtg ctgtactgct tcccgacccc gctgttctat
     5641 gaaatcgcac agacctcagg ctttttgtgc attaccgcgg gagcgtattt catgctgaag
     5701 tcaaatgtaa taggggaggg ggaaataagg ctgccgtcgc tttgcctttc aagcaccttc
     5761 cttgccctgg cggtgctttg cagacccacc ctcgccgttt attcggttgt ggctctgctg
     5821 ttcatcttcg ccggctttaa taagctgagg ggcaaggttg gaaacaaggc ttacaaggat
     5881 ttcataaaat acgccgccgc gtcgctcatt cccttcgctt tcttcggcgg aattcagatg
     5941 atttacaatt atttaagatt cgattctccc cttgatttcg gaattcagta ttccttaacc
     6001 atcaacgact ttacaagggc tgagtttcat gttcaccttg cggcaattgg cttcttcaac
     6061 ttcctctttg ccttcccgga gttcagcccc gacttcccgt tctttgtgtc aagggttcag
     6121 accttttcgc ccaacggata ttatttcgtt gccaccaata cggcaatcgg tctaatcttt
     6181 aaggctatgc ccgttctgtc tttaggctat tggagaaagg cgtataagaa ttccgattca
     6241 aagaacaaaa agctttacac cgctctcttg ttcgcgctcg gaatcgccgc gccctttata
     6301 atcatagcct caatttggga gagcggctac ggagtaagat acggcgccga ctttaactgg
     6361 cagattcttg tttgcgcctt cgcgataatg tttacaattt ataatagact gcagaacaaa
     6421 ggcgtaaaga aaatcttaac tcagcttttc gtattttcgg ttgtcgtatc ggtaatactt
     6481 gtaaccaccg agctttggtc atggcaggtt tacaacaaac cctcggatga aattctcaga
     6541 cttcttttct ccttcggcag aatatttgag ttctggaaat aacaaaaaat ctttcaaatt
     6601 cctcgaaaat cttcaaatct ttagcaaata ataaaaataa ctattgcaac acattgtaat
     6661 atgtgatatt atatttcagc gttcgtttgc ggatattgcg acaaacggag tcaaaagctc
     6721 tccgcttttg aacaggcgtt ccgctgcatt tgcaagaacg gtcgaattaa taggaggtta
     6781 ttatggacgc acttaaaatc attgaagcag gtcagcttaa ggaaaatgtt cctgagttcg
     6841 ctgtcggcga tactgtaaga gttcatgtta aaatccgtga gggtgagcgt gaaagaattc
     6901 aggcctttga aggcacagta attgcgcgca agggcagcgg agtttccgag acctttaccg
     6961 tcagacgcgt ttcctacggc gtaggcgttg aaagagtatt ccctctccat tcacccaatg
     7021 tagccaatgt tgaggttgta cgccgcggcc gtgttcgcag agctaagctc tactatctgc
     7081 gtgaccgcgt cggtaaggct gctaagatta aagagcagat ctaaattttg cttgcaagca
     7141 ctgaaggggc acttgatgtc ccttttttgc ttttttaggc tattgcaaat taagtccaat
     7201 agatttataa ttttatggaa tctttgtaag ggggcgatat tttgcgtcgg cgtgaagact
     7261 atattataga gttcgactac gaagaatcag agccgcagca aaagcctccc ggccgctttt
     7321 ggctttatgt ttacgacttt ttcggctcaa tatcgggcgc cctttttacg gtttttatag
     7381 tctttacttt tttgttcaga atggtggatg ttgacggcga ttccatgctg aacacccttt
     7441 ccgacggcga ctggctgtgt atttcgagcc tttattttga gcccgaatac ggcgatattg
     7501 tcattatcag cagggaaaat actaacgagc ccccgattgt aaaaaggata ataggcaaag
     7561 aaaacgatgt tatagatata gacttccaca ataacaccgt ttcggtaaac ggcaaaatcc
     7621 tcgatgagcc ttatgtttca accccgaccc aacgctcttt tgatgtgagc tttcctctta
     7681 ccgttcccga aaactgcgta tttgtgctgg gggacaacag aaaccactcc cttgacagca
     7741 ggtcctctct ggtcggctgc gtggatgaaa accgcatttt gggcaaagtt cttgtaaggc
     7801 tcactccaga gattactttc ttcatcggac agaaagggca ttgatttatg aaggataaga
     7861 tagtggatat ggcgtatgag gtcgcctccg tcctcgtatc gtcaattatc ataatagcga
     7921 taattttcac ctttgctttc cgccttgtgg gcgtttcggg gcactcaatg gacacaaccc
     7981 tttcccacgg cgactggctt cttgtcacca ccacatacac agagcccaaa tacggcgaca
     8041 ttgttgtcat tgcccagccc acatacttta aagagccgct tatcaaaagg gtaatagctg
     8101 tcggcggcca gacggtggat atagacaacg aaaccggaac cgtttatgtg gacagcaagg
     8161 ctttagacga gccctatatt tcaactccca caaccaatcc aaccgtttcc cagatagatt
     8221 tccccgttgt ggtcccgtat ggttatcttt tctgcatggg tgacaacaga aacaactcca
     8281 cagacagccg ctcaagcctt gtgggcttta tagatgaaag atatatcctc ggcaaagccc
     8341 tcaaaagggt agtgcccttt ggtaaatgga atatctacta aagaataaac cgcctgatat
     8401 aagtcaggcg attttttatg cataaaaaaa gagctgtgaa aaatcacagc tcctaatttt
     8461 tgatttctta tttaatcatg ctggaaactt cctcagcgaa gttgtcctct ttcttctgga
     8521 tgccctcgcc cttagcatag cgatagaagg cagatactgc aacgggagcg ccaacctgct
     8581 ttgcgacgga ctcgatatac tgagcgacgg tgatatcctc gtccttaaca aactgctgct
     8641 ctaagaggca ggtctcctta aagtacttgg agagcttgcc cttaacgatg ccctcgataa
     8701 ccttctcggg cttgttggcc atcttctcgt cagccttaat ctgagccata aggatgccct
     8761 tttcatgctc gataacatcg gcgggaacat cggactcttt aagatacagc gggctcatag
     8821 cggcaatctg cattgcgaca tccttgccca tagcgatgac ttcttcctta gcagctgtct
     8881 ctttgtcagc ttcaagaaca acgataacgc caactgtgcc gccgccgtgg atatactcaa
     8941 ctgtgttgcc ctcaactctt acaaagcggc gaaccttaat gttctcaccg attgaaagaa
     9001 ccttatcctg aacatgctcc tgaactgtac ggtcggaacc gacaagattt gttgcgagga
     9061 gagtctcaac atcagcgggg ttggtcttag cgattgtctt agcgacatca agaacgaaag
     9121 ctctgaattc ctcgttcttt gctacgaagt cggtctcgct gttaacctca acaagaacgc
     9181 tgacgcctaa atcattatct ctataagcaa gaactacgcc ttcggcagcg attctgcctg
     9241 ccttcttgtt agcggctgca agtccctttt ctctaagaat gtcaattgcc ttgtccatat
     9301 cgccgttggc ctcaacaaga gcctttttgc actccatcat gccaacgcct gttttctctc
     9361 ttaatgcctg aacatctttt gcggtgaacg acatggtaaa tcctcctgtt cattctatta
     9421 tttaaccgag ctgcatttag ggcagtcgga aaatctttga aacttctgat tcaaaataaa
     9481 gcccgcactt tggtacgggc ttgaatttca gtaaattact ctgcgtctgc agcttcgaca
     9541 gcctcttcag cctcggcagc ttctacagct tcagcagcct cttcagcgtt ctcgtcgcct
     9601 tctgctgcgg gagcaaactg ttcgccctgg cggccttcaa gaaccgcgtc ggcaatagcg
     9661 cctgcgatga gcttaacagc tctgatagcg tcatcgttgc cggggattac atagtcgata
     9721 aggtcggggt cgcagtttgt gtcaacaata gctataatcg gaatgccgag cttccttgct
     9781 tctgctactg cgattttctc cttgcggggg tcaaccacga acatagcagc gggcatgcgg
     9841 cgcatttctg tgatgccgcc aaggaatttc tcaagcttct ctatttgcag cttcagctta
     9901 acaacttcct ttttggggag aaggtcgaat gtgccgttct cttccatagc cttaagctga
     9961 gcgagtctct ttatgcgcag tttaattgta ttgaagtttg taagcatacc gcccagccag
    10021 cgagcgttaa catagggcat attgcatctt tcggcttctt ccttaatgga ttcctgggcc
    10081 tgcttctttg tgcctacaaa gagaatttct tcaccgtttg cggcgatttc gcgaacgaat
    10141 gcataagcct cttcgagctt cttaactgtc ttctgaaggt cgatgatata gattccgttg
    10201 cgctctgtga agatgtactt agccattttg gggttccatc ttcttgtctg atgcccgaag
    10261 tgaacgccgg cctcgagcaa ctgcttcatt gatactactg ccattaatta gcctcctttt
    10321 gttttgccgc cgcagccctc aacttacctt gcgacataga tatgcacaac aagataatca
    10381 gactgcgtgt gttttgcctt gaaagtataa cacgacattt tattatttgc aagaattttc
    10441 tttaattttc tcagaaataa gagatttccg cgagggattc gcatatattt cgaaaaatcg
    10501 gagcttttta agtcttatat tcgtacatca gccttaccgc gttccttatt tctttgtagc
    10561 ttgtgcagcg gcagagattg gactgcagcc attcctctac aatatgctca tctggataag
    10621 tggggtagtt ctgccttaaa gcctcgcaga ccatcaaaaa accgcttgtg cagtagccgc
    10681 actgaaaagc gtttgcgtcc acaaatgccc tttgagcggg tgactgaccg cgcagcccct
    10741 ctacggtttg gattttgtga ttctccgcct cgaccgcgag cacaagacag gactttgcgg
    10801 gcaggccgtc aattataatt gtgcaggttc cgcagtcgcc gtttttgcac ccaggttttg
    10861 ccgccgtaag accgagcttt tgacgaagca catcaagaag cacatcggag ggtctgacag
    10921 ccgctttgtg aacctcgccg ttaatgtcga gcgtcacgac caccttgtcc tcgtacttaa
    10981 ccattttccc actcctccaa aagctcttct attgcattct taaaaacgaa ccttctatat
    11041 tccgctgagc ctgctctgtc gctgtattcc tcgggtatca gcgacgctgc cctttccgcc
    11101 ctttcggata tggacaaaga tttgttgttt aacacaagct caatctgttg actgcggaag
    11161 ggaaaggagc aaacccccga aaaggcaatt ctcagaaagt tatcttttat aagtgccgct
    11221 atggatacaa gagggtagtc tattttctcc tgcgcggtaa gttttatatg aaaatgctgt
    11281 gccgaaagag cccatttcgg aatttgcgcc gacaccaaaa gctcactggg caggagatgt
    11341 attcttccat caaaaacatc ggagaaggga actgtccttt ttccctctgg acctaaaaga
    11401 gtaacctccg catccgataa aagcagaggc ggaactgttt ccttatatat aatagtgccg
    11461 cagatattgc cgccgagggt gattctgcac tgatttgtat ggtccgcaat ccttccgcat
    11521 gcaagagcca aaaggggata tgcctttgac tgcttaatct tatacaaagt gcatgcggag
    11581 ccaatgcgaa gacagctttc atccatggca agacttttta attcggggat ttctttaata
    11641 tctatcacag cgtcgggtct tatgcttccc gcgcggcaca tagtgatgat ttcgctgcct
    11701 cccgaataat aaactgcgct tagacctttt gaagtaagct ctgaatgaat ccgaaccgct
    11761 tcctctaaag aatcgggcct gtaataagtg aaattaaaag gtatcatttt ctttccccgc
    11821 agcataaaaa tatcagtcat tcaaactttt cccaaatatt tcgatactta ttcaaagccg
    11881 cggaaaacaa taggatatga aaaattaaaa cccgtcagcc taaacttaaa gactgacggg
    11941 ttttctggtc ggagtgccgg gatttgaacc cggggcctct tggtcccgaa
//
LOCUS       DAONVQ010000058        11765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4207_length_11765_cov_4.838514, whole genome shotgun sequence.
ACCESSION   DAONVQ010000058 DAONVQ010000000
VERSION     DAONVQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11765
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4207_length_11765_cov_4.838514"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1098
                     /locus_tag="PLD98_05030"
     CDS             145..1098
                     /locus_tag="PLD98_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HQA05646.1"
                     /translation="MKIVILDGHTTNPGDLSWAFLNEYGDVSIYDRTPKELIIERSLP
                     ADILITNKTPLPKSILEQLPNLKYVALLSTGYNIADTDYLKERGIPVSNIPDYSTEAV
                     AQHVFAFILEHYNSVALHSEAVRGGEWSICPDFCFLKGPVMELMGKTIGIIGFGKIGR
                     SVAEKALAFKMNVIAYTPNPKKDADFVEFVSLDELAERSDIVTIHTPLTDATRGFINR
                     DFIFKMKKGAFLVNTSRGPVIDEQALADALNSGHLAGAGLDVLSTEPPSKDNPLLTAQ
                     NCFITPHIAWAGRETRARLLSILNENIKAFVNGKPQNVVNM"
     gene            complement(1140..1922)
                     /locus_tag="PLD98_05035"
     CDS             complement(1140..1922)
                     /locus_tag="PLD98_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05647.1"
                     /translation="MKKPTFGGLNELLEAIKLRYSLIEALGINEKNEKELREKINALF
                     LHARLCKDSISSEEELFDLLEIMNACRKKLRSMNNYSLSKLKVCDYKSFDPEYFLEDI
                     FVAADLMLSSFGKSIEFKAEGRIPEISANPEVLFTAVMNLISNAAIHSRGELISASLS
                     MAGNRACLKVENEGRLNLNMLRQAYNRGGSGVNCIMNAARLHCGSFLLGVSGNRTKAV
                     LSISSMEAQSTGAYKLPDFTDYICDRLSPMYTALCDCVLCPL"
     gene            2211..3224
                     /gene="gap"
                     /locus_tag="PLD98_05040"
     CDS             2211..3224
                     /gene="gap"
                     /locus_tag="PLD98_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010167242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQA05648.1"
                     /translation="MSVKVAINGFGRIGRLAFRQMFGAEGYEIVAINDLTDAKMLAHL
                     LKYDSAQGRYALADKVQATENSIIVDGKEIRIYAEKDAANLPWGELDVDVVLECTGFY
                     TSKAKSEAHIKAGAKKVVISAPAGNDLPTIVYGVNEKTLTPEDTIISAASCTTNCLAP
                     MAYVLNKTVEIEKGFMTTVHAFTGDQMVLDGPHRKGDLRRARAASANIVPNSTGAAKA
                     IGLVIPELSGKLDGAAQRVPVITGSITELVAVVKGTVTAADINAAMKAAQSESFGYTE
                     EELVSSDIIGMTYGSLFDATQTKVTPLEDGKSLVKVVAWYDNENSYTSQMVRTIKYFA
                     ELK"
     misc_feature    3327..3473
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3509..4033
                     /gene="rplJ"
                     /locus_tag="PLD98_05045"
     CDS             3509..4033
                     /gene="rplJ"
                     /locus_tag="PLD98_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQA05649.1"
                     /translation="MEQKQQQVAEISEKISKSVAGVLVDYKGITVAEDTALRAELRKA
                     GVHYEVIKNTLLRLAIKDTPLENLDEVLSGTTAIALSEDDHIAPARIITKFASKHKTF
                     QIKGGFLDGEVISLEKIDSLAKLPTREVLLATVVGAFQAPMAAFARAIQAIIDKQGEA
                     AAPAEAAAEAPAEA"
     gene            4095..4475
                     /gene="rplL"
                     /locus_tag="PLD98_05050"
     CDS             4095..4475
                     /gene="rplL"
                     /locus_tag="PLD98_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQA05650.1"
                     /translation="MASEKVVSIVNEVEKLTVLELAELVKEIEERFGVSAAAAVAVAA
                     PAAAEGGAAAEEKTEFDVILAEVGAEKIKVIKAVRELTGLGLAEAKALVDGAPKPVKE
                     GVSKDDAEAAKAKLEEAGAKVEIK"
     gene            4576..4767
                     /locus_tag="PLD98_05055"
     CDS             4576..4767
                     /locus_tag="PLD98_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05651.1"
                     /translation="MRRLLCGAVFAAAGGGSEGGYTTLSGGQVGSAVVGGSPIRPPPQ
                     GGCHAGWGKVSAGGYSCSV"
     gene            complement(5016..5756)
                     /locus_tag="PLD98_05060"
     CDS             complement(5016..5756)
                     /locus_tag="PLD98_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQA05652.1"
                     /translation="MSKILFIGAHNDECEYGTGGIAALLSHAGHEVMFLNVACKRHSV
                     RTPERLKVEEEMERRAAEILGAKKKIVGDRSSSAYFQTPENTEIIMNEIIAFKPDIVF
                     MHWPKDNHIEHREVSRATLDALCLAAVRCVVPSETYAFEAGPDQTMQYFQPDIYIDIT
                     EAAPLVEKSLKTFDMEHANGEELWREKEVCARFRGHLAGVKYAETLKIIKFPTGFEEN
                     ELLLPKLLKGKFSWHNVGMYPWGRQYFL"
     gene            complement(5813..6619)
                     /locus_tag="PLD98_05065"
     CDS             complement(5813..6619)
                     /locus_tag="PLD98_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05653.1"
                     /translation="MADNLTLMTNKIFLNAALPLVKVIATDVPSLAKKFKNIHAVIQV
                     SALDSSEPHGKAATHFVVNAGEWLVHADKVSTKVNVELQFKSREAMNDFFKGKISPAT
                     LPKMIGVTKNLKAFIAFMSVLLKMSSLLGATQPPEDYEEKKLLVKCFFYLLSSGISQL
                     NKSGHEAVHSWALKSPDRVYAWAVDGEPEVSAYLRVKAGKTKAGRGEYKRSMPFFKMR
                     FDSLDSALGILMQTDDMLESTRQGKLIMDGAPEFGAQIGEFMMLVGELAK"
     gene            complement(6652..7662)
                     /locus_tag="PLD98_05070"
     CDS             complement(6652..7662)
                     /locus_tag="PLD98_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQA05654.1"
                     /translation="MAGGLTYTAVDSTSLSTAEIYGKALVELGREHPEVVALTADLAK
                     STKIGDFMKEFPERFFNVGIAEQNLLGVAAGMAKSGLVPFASTFSQFASFRAADQLHT
                     DICYQNVNVKVIATHSGTSFGQAGSTHHAICDLAVTRSIPNLTVIVPADGVETYNAVM
                     AAYETPGPFYIRINRGFDRVLYKDSNYGFEVGKAVTVKEGTDITVIACGSCVFQALQA
                     AEFLEKTDGLKVRVLDMHTIKPIDKEAIQKAVFETRRIITVEDHNILGGLGSAVAEVV
                     VDYGKACAFKKLGIPDKFAPIGLHEDIMSIVGIDSNGIIEAVREVMGKDFELDDDWDD
                     EV"
     gene            complement(7665..8534)
                     /locus_tag="PLD98_05075"
     CDS             complement(7665..8534)
                     /locus_tag="PLD98_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQA05655.1"
                     /translation="MALKKADWLALEKKAHELRRLTLDTTFWAGSGHIGGGMSVLDML
                     TILYHRYMNIKLEDPNWEDRDRLVLSKGHAGIAYAPVLCDLGWNDMEQLKTFNHTNSL
                     MGMHLDSNKVVGVDASTGSLGHGLSIAVGMALAAKVLGKTYKTYCILGDGECDEGSNW
                     EAAMTAAHYKVNNLITFVDRNKCMIDGKTEDVMGLEPFADKWKSFGFIVKEVDGHNFN
                     ELSEAIDFALNNDGAPVVIIAHTIKGAGIDFMEDNYVWHYGAIDSDKYEDAKKSLDAY
                     YKKRVERAEREGA"
     gene            complement(8589..9008)
                     /locus_tag="PLD98_05080"
     CDS             complement(8589..9008)
                     /locus_tag="PLD98_05080"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HQA05656.1"
                     /translation="MFYGNEYEAVTSYSAIRDSVAGFMFFALIFTSVTFFISFIPMII
                     AYVRKHPKRQFIAIISFIVPPLGFIIALIWALTDTDPENQWFSNLFSSRKKEPDDTPR
                     PDPQLLKALFHLDSLYRQGILTEEEYKEKRAEIIKRL"
     gene            complement(9030..10292)
                     /locus_tag="PLD98_05085"
     CDS             complement(9030..10292)
                     /locus_tag="PLD98_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQA05657.1"
                     /translation="MAREINKYDKSREWFKRAVEVIPSGVYGHLGPAEGCFIPVDAFP
                     LFSERAQGAYFWDVDGNRFIDYMCAYGPNVLGYGDPDVDNAAIEQLKKANCTTSPSYK
                     MVELAELLVDTVHSADWAFFAKNGGDVTTLAIMTARAATHRKKIVFTKGFYHGVAPWT
                     QKIDYPGVLPEDVANNIYIPWNDADAFEKVIAENEGEIAAFISTPYMHGNFVDNVLPA
                     EGYWKRIRELCDKHGIVLICDDVRCGFRLDLAGSDHYFGFEADLICFCKAIANGWNMS
                     ALCGKEHLKSVVSGITYTGSYWLSAAPFAAAIATINKLKAIDGPKMFKEKGTLLAEGL
                     KAAAANNGFDLIVSGEPALFYLRIANDDSLMLHQEWVAECVKRGVFFTNHHNHFINAA
                     LTEEDIAETIEVAEEAFKVVKRNHPELQ"
     gene            complement(10305..11066)
                     /locus_tag="PLD98_05090"
     CDS             complement(10305..11066)
                     /locus_tag="PLD98_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05658.1"
                     /translation="MTDAKTLAYINMYAVLGTLENLCELDPKASALLTNKKPISIAFD
                     VKGGPVATITFKNGKCRMVDSILPDADIKIPFSSPEKFNGLIDGTVTPIPTKGFTKIP
                     FLLKEFKKLTDLLSSYMRAEPEKLEDEEFFRISTSLMFYTICVAVAQIGNNDEIGKFS
                     ASHMVDGDIMISIADGPAATVRVRNHRLVTIKKAPENPRAVMEFSSIRLARDLFDGKV
                     NAVACIGDGSIRMRGMISMVDNLNRILDRVGLYLA"
BASE COUNT         2875 a         2840 c         3000 g         3050 t
ORIGIN      
        1 ataaatgctt aaaaaggtca attgatgggg aatatttata ggacaaatat gagaaaaaag
       61 gttcatcctg tcgttttata tgctaaaata atgtataata tacattatac cgaaagcgga
      121 aacttatgat tgcgaggttt ctttatgaag atagtgatac ttgacggtca taccacgaac
      181 cccggcgacc tttcatgggc ttttttaaat gaatacggcg atgtttcaat ctacgacaga
      241 actcccaagg agcttattat cgaaaggtca ctgcccgcgg atatactcat aacaaacaaa
      301 actccgctgc ccaaaagcat tctggagcag ctgccgaatc ttaaatatgt agccctgctt
      361 tcaacgggct acaatattgc cgatacagac tatttaaaag agaggggcat acccgtctca
      421 aacatccccg actactccac ggaggcggtg gctcagcatg tctttgcctt tattctggaa
      481 cactacaaca gcgttgctct gcacagcgag gcggtgcgcg gcggagagtg gagcatctgc
      541 cccgactttt gcttcttaaa agggcccgtt atggagctta tgggcaagac aataggaatt
      601 atcggctttg gcaaaatagg caggtcggtg gctgagaagg ctttggcttt taagatgaat
      661 gttatcgcct acacgccaaa ccccaagaag gacgcagact ttgttgaatt tgtttctctc
      721 gatgagcttg cggaaaggtc cgacattgtt acaatacata ccccgctgac agacgcaaca
      781 cggggcttta taaatcgtga ctttatcttc aaaatgaaaa agggcgcttt ccttgttaat
      841 acttcaaggg gccccgtaat tgacgagcag gcgctggcag acgcactgaa cagcggtcac
      901 ctggcgggag cgggtcttga tgtgctcagc accgagccgc cgtcaaagga taatccgctg
      961 cttacagcgc aaaactgctt tatcactccc catatcgcat gggcgggaag ggaaacaagg
     1021 gcaagactcc tcagcatcct taacgaaaac attaaagcct ttgtgaacgg aaagcctcag
     1081 aatgtggtga atatgtaaac taaaaaagcc ggcaaaacgc cggctgtatt ttttgagctt
     1141 tacaagggac acagcacgca gtcgcaaagg gccgtataca tgggcgacag gcggtcgcag
     1201 atatagtcgg taaagtcggg aagtttgtat gctcccgtgc tttgcgcctc catagatgaa
     1261 atggataaaa cggctttggt tctgttcccg ctgacaccca aaaggaagga gccgcagtgc
     1321 agccgcgcgg cgttcattat gcagtttacg ccgctgccgc cgcggttata tgcctgcctg
     1381 agcatattca agtttaatct gccttcattc tccaccttaa gacacgctct gtttcccgcc
     1441 atgctgagag acgccgaaat aagctctccc cttgaatgaa tggccgcgtt tgaaataagg
     1501 ttcatgacgg cggtaaagag cacctccgga ttcgcgctga tttcggggat tcttccctca
     1561 gccttaaatt caatgctctt tccgaaggaa gaaagcatca aatccgccgc cacaaaaatg
     1621 tcttccagga aatactccgg gtcaaagctc ttgtagtcgc atactttcag ctttgacagg
     1681 gaataattat tcattgagcg aagttttttg cggcaagcgt tcattatttc caaaaggtcg
     1741 aacagctctt cctcggacga aatactgtcc ttgcagagcc ttgcgtgcaa aaacaaggcg
     1801 tttatttttt cccgcagctc tttttcattc ttctcattga ttcccagggc ttctatcagc
     1861 gaatatctca gcttaatggc ttcaagcaat tcgttcagtc ctccaaaggt gggtttcttc
     1921 aaactattat gcccacagag attggaaaat aataatcggc gaaaaatttt attcgtttaa
     1981 tggctttaaa tatttctcta attgttaaaa aattgactat ttagccaaaa ttttattgca
     2041 aaatgccgaa tatgcgtcgt aaggcctttt tgttgaagca aagctattgc atatttcttc
     2101 atttggatat aaaatattaa actgtaagaa aggattaacc taaaatttcc tatggctctg
     2161 ccgtaggtgg gagttacatc cttaacaaat taataacgga ggaataaatt atgtctgtta
     2221 aagttgcgat taacggtttt ggccgtatcg gtcgtcttgc attccgccag atgttcggcg
     2281 cagagggcta tgaaatcgta gccatcaacg accttacaga tgcaaaaatg cttgctcacc
     2341 ttttaaagta tgactcggct cagggccgct acgctcttgc cgacaaggtt caggctacag
     2401 agaattcaat catcgttgac ggcaaggaaa tcagaatcta tgccgagaag gacgccgcaa
     2461 accttccttg gggcgagctt gatgttgatg tagttcttga gtgcacaggc ttctacacct
     2521 caaaggctaa gtcagaggct cacattaagg caggcgctaa gaaggttgtt atctccgctc
     2581 ccgcaggaaa cgaccttccc acaatcgttt acggcgtaaa tgagaagacc ctcacacccg
     2641 aggacaccat catctccgca gcatcctgca ccacaaactg ccttgctccc atggcttatg
     2701 ttcttaacaa gacagttgaa atcgagaagg gctttatgac caccgttcac gccttcacag
     2761 gcgatcagat ggttcttgac ggcccgcacc gcaagggcga cctgagaaga gcaagagccg
     2821 cttccgctaa catcgttccc aactcaacag gcgcagcaaa ggctatcggc cttgttatcc
     2881 ccgagctttc cggcaagctt gacggcgctg cacagcgtgt tcccgtcatc accggttcaa
     2941 tcacagagct tgtagcagtt gttaagggca cagtcacagc cgctgacatc aacgcagcta
     3001 tgaaggcagc tcagtccgag tccttcggct acaccgagga ggagctcgta tcctctgaca
     3061 tcatcggcat gacctacggc tcactctttg acgctaccca gacaaaggta actcctcttg
     3121 aagacggcaa gagccttgtt aaggttgttg catggtacga caacgagaac tcctacacct
     3181 ctcagatggt aaggacaatc aagtactttg cagagctgaa ataagcgtcc attagaaatt
     3241 gtaattagcg gccgcccgtc ctatgcatgg gcggccggtt ttcaaaaaat tttataaaaa
     3301 aacttgacat tttgtgtaac gaatgctaca atgacaaagc tgttcttaag acagcaggtg
     3361 ctttatgcat aatggctttg ccgcctgccg aggaatagag cttaaattga atcttatttt
     3421 ggattaatta tgctctttcc acggtttgcg gaaagagctt taatattttt gttaaggcag
     3481 gtgaaaggat ttgccaagcg caaaagtttt ggaacaaaaa caacagcagg tagcagaaat
     3541 ctctgagaag atatcaaagt ccgttgcagg cgttcttgtt gactataagg gaatcactgt
     3601 tgcagaggat acagccctca gagcggagct cagaaaagcg ggcgttcact acgaggttat
     3661 taagaatacc cttctgcgcc ttgctattaa ggacactccc cttgagaacc tggacgaggt
     3721 cctttcaggc acaacagcaa tcgctctgag cgaggacgac cacatcgctc ccgcaagaat
     3781 catcacaaag ttcgcaagca agcataagac cttccagatt aagggcggct tcctcgacgg
     3841 tgaggttatc agccttgaga agattgacag ccttgcaaag ctgcccacaa gagaagttct
     3901 tctcgctacc gttgtcggcg ccttccaggc tcccatggcc gcatttgccc gcgctattca
     3961 ggctattatc gacaagcagg gtgaagcagc cgctcccgcc gaagctgcgg cagaagctcc
     4021 cgcagaggca taaactcagc atataagtaa attcaataca gcctatatgg caaataaaac
     4081 aggaggaaac aatcatggca agtgaaaaag ttgtcagcat agttaatgaa gttgaaaagt
     4141 taacagttct cgagcttgca gagcttgtta aggaaatcga agagagattt ggcgtatccg
     4201 cagcagccgc tgtcgctgta gcagctcccg ctgcagctga gggtggcgca gccgctgagg
     4261 agaagacaga gttcgatgtt atccttgcag aagtcggcgc agagaagatt aaggtcatca
     4321 aggctgttcg tgagctcaca ggtctcggcc ttgcagaggc taaggctctc gttgacggcg
     4381 ctccgaagcc ggttaaggaa ggcgtttcca aggatgacgc cgaggctgct aaggctaagc
     4441 tcgaagaagc aggcgcaaag gttgaaatta aataagaaag tcaactcatt aacaaacagg
     4501 gacacattcg tgtccctgta tttttttgcg cttttggacg gtgaacttta tcaggtcggc
     4561 gccacggagc ggcgggtgcg gaggctccta tgcggcgcgg tttttgcggc tgcgggcggc
     4621 gggtctgagg gcgggtacac taccctcagc ggcgggcagg tcggctctgc cgtcgtcggc
     4681 gggtcaccca tacgacctcc cccgcagggc ggctgccatg cggggtgggg aaaggtatcg
     4741 gcgggcggat attcctgctc tgtttgaggt tgtggcgcgg atgacgcagt gcggagggga
     4801 aaagccgcgc gcctgtgggg gcggggtgcg gaattctcta tgggcaggcg gcgggctctc
     4861 tggcggcggg tcgctcaaaa gaaaaaaccc cgcagggcgg ctgccatgcg gggtggggtg
     4921 ggacggtacg acggtgagcg ggaggatgct ttttcggaca gtctttttaa gaaaataaaa
     4981 agcacagcag ggctgtgctt taccttagct gggctttaga gaaaatactg tcgtccccag
     5041 gggtacatgc cgacattgtg ccaggagaat ttgcctttca gaagttttgg gagcaaaagc
     5101 tcgttttcct caaagcctgt cgggaatttt attatcttta gcgtctctgc gtattttacg
     5161 cccgcaaggt ggcctctgaa ccttgcgcag acttcctttt ccctccaaag ctcctcgccg
     5221 ttcgcgtgct ccatgtcaaa ggttttaagg ctcttttcca caaggggagc cgcttccgtt
     5281 atatcaatat agatgtctgg ctgaaaatac tgcatagtct ggtcgggacc cgcctcaaaa
     5341 gcgtaggttt cgctcggaac cacgcatctg accgccgcaa ggcagagagc gtcaagggtc
     5401 gcgcgcgaga cctccctatg ctcgatatga ttgtcctttg gccagtgcat aaagactatg
     5461 tcgggcttga aagcgattat ctcgttcatt atgatttccg tgttttcggg ggtttgaaag
     5521 taagcagagg agcttctgtc cccgacgatt ttctttttcg cgcccaagat ttccgcggct
     5581 cttctttcca tctcctcctc gactttgagc ctttcgggag ttctcactga gtggcgtttg
     5641 caagccacat ttaaaaacat tacctcatga ccggcgtgtg acagcagcgc ggcgattccg
     5701 cccgtgccgt actcgcactc gtcgttatgg gcgccgataa aaaggatttt actcattaaa
     5761 tcaccttaaa aaacagggga gataaactcc cctgtgattg gtttagcggg ttttattttg
     5821 ccagctcgcc cacaagcatc ataaactcgc cgatttgggc gccgaattcg ggagcgccgt
     5881 ccattatcag cttgccctgt cttgtggact ccagcatatc gtccgtctgc ataagtattc
     5941 caagggcgga gtcaagggag tcaaagcgca tcttgaagaa gggcattgac cttttgtact
     6001 ctcccctgcc tgcctttgtc ttgcctgcct ttacgcgcag gtatgccgat acctcgggct
     6061 cgccgtcaac agcccacgca tagacgcggt cggggctctt gagcgcccat gaatggacag
     6121 cctcgtgacc gctcttgtta agctggctga taccgctgga cagaaggtag aagaagcact
     6181 ttacaagcag ctttttctcc tcgtaatcct cgggcggctg agtggcgccc agaagggacg
     6241 acatcttcag aagcaccgac ataaaggcga tgaatgcctt taggttcttg gtgactccta
     6301 tcatcttggg cagtgtggcg ggggaaatct tgcccttgaa gaagtcgttc atagcctctc
     6361 tgctcttaaa ctgaagctcc acatttacct ttgtggagac cttgtcggcg tgaacaagcc
     6421 actcccctgc gttgaccacg aaatgggttg ccgctttgcc gtggggctca gagctgtcaa
     6481 gagcgctgac ctgaattacg gcgtgtatgt ttttaaactt cttcgcaagg gacgggacat
     6541 cggtggcaat gaccttgaca agaggcaggg cggcgttaag aaatatttta ttcgtcatca
     6601 atgttaaatt atcagccata ttatcctcct tattttaaat ttttaaggag cttagacctc
     6661 atcgtcccag tcgtcatcaa gctcaaagtc tttgcccatt acctctctta cggcctcgat
     6721 tatgccgttg gagtcgatgc ccacgatgga cataatatcc tcatgcagac cgatgggggc
     6781 gaacttgtcg ggaatgccga gtttcttgaa ggcgcatgcc ttgccgtagt caacaacgac
     6841 ctcggcaacg gcggagccga gaccgccgag aatattgtgg tcctcaacgg tgatgattct
     6901 tcttgtctcg aatactgcct tctggatagc ctctttgtcg atgggcttga tggtgtgcat
     6961 atcgagaact ctgactttca gtccgtcggt cttctcaagg aactcagcgg cctgcagagc
     7021 ctggaatacg catgagccgc aggcgatgac ggtgatgtcg gtgccttcct ttacggtgac
     7081 agccttgccg acctcaaagc cgtagttgga atccttatag agcaccctgt caaagcctct
     7141 gttaatgcgg atgtagaagg ggccgggtgt ttcgtatgcc gccattacgg cattgtaggt
     7201 ttccacgccg tcggcgggaa ctataactgt aagattcggg atggaccttg taacggctaa
     7261 gtcgcagatt gcgtggtgag tggagcctgc ctggccgaag gaggttcccg aatgggttgc
     7321 gataaccttg acatttacat tctgatagca aatgtcggtg tggagctggt cagcggctct
     7381 gaaggaggcg aactgcgaga aggttgaagc aaagggaaca agacccgact ttgccatacc
     7441 tgccgcaacg ccgaggaggt tctgctcggc aatgcccaca ttgaagaaac gctcggggaa
     7501 ctccttcatg aagtcgccga ttttggttga ctttgcgagg tcagcggtca gagcaacgac
     7561 ctcgggatgc tctctgccca gctctaccaa tgcttttccg taaatctcag cagttgaaag
     7621 agatgtggaa tcaacggctg tgtaagtaag tcctcctgcc ataattatgc tccctccctt
     7681 tctgcgcgct ctacacgctt cttgtagtat gcgtcaaggc tcttctttgc gtcctcgtac
     7741 ttgtcgctgt caatggcgcc gtagtgccat acatagttgt cctccataaa gtcgatgcct
     7801 gcgcccttta tggtgtgggc tatgattact acgggagcgc cgtcgttgtt aagagcaaag
     7861 tcaatagcct cggaaagttc gttgaagtta tgtccgtcga cctcttttac gatgaagccg
     7921 aatgacttcc acttgtcggc aaagggctcg agtcccatga catcttctgt cttgccgtca
     7981 atcatgcact tgttgcggtc aacgaatgta ataaggttgt ttaccttata gtgggcggcg
     8041 gtcattgctg cctcccagtt ggagccctcg tcgcactcgc cgtcgccgag gatgcagtat
     8101 gttttatagg ttttgcccag aacctttgcc gcaagagcca tgcccacggc gattgaaagg
     8161 ccgtgtccca gtgagccagt ggaggcgtca acgcccacaa ccttattgga gtcaaggtgc
     8221 atacccataa gggagttggt gtggttaaag gttttgagct gctccatgtc gttccagccc
     8281 aaatcgcaga gaacgggggc ataggctatg ccggcgtgac ctttgctgag gacaagacgg
     8341 tctctgtcct cccagttggg gtcttcaagc ttaatgttca tataacggtg ataaagaatt
     8401 gtgagcatat caagaacgct catgccgcca ccaatatgtc cgctgccggc ccaaaaggtg
     8461 gtatcaaggg taagtcgcct cagctcgtga gccttttttt caagggcgag ccaatcggct
     8521 tttttcagtg ccattgattt acctccagtc cattaatttt gtttagcaag taagggtcag
     8581 cgccgcattt aaagtctttt gattatttcc gcccgctttt ctttgtactc ctcctcggtc
     8641 agaatcccct gcctgtataa agagtcaagg tgaaacaatg cctttaaaag ctgggggtcg
     8701 ggcctcggag tgtcgtccgg ctcttttttg cgggaggaaa agagatttga gaaccactga
     8761 ttttccgggt ctgtgtcagt aagcgcccag ataagggcta ttatgaatcc gaggggaggt
     8821 acaatgaagc tgattatggc gataaactgc cttttggggt gctttcgcac ataggcaatt
     8881 atcatgggaa tgaaggaaat gaagaaggta acggaagtga atataagggc aaagaacata
     8941 aatcccgcaa cagagtccct tatagcactg tagcttgtta ccgcttcgta ctcattgccg
     9001 tagaacactt tgtacctccc gcttatcact tactgaagct cagggtgatt tctctttact
     9061 accttgaagg cttcctcagc gacttctatg gtttctgcaa tgtcctcttc ggtcagagcg
     9121 gcgttgatga agtggttgtg atggttagtg aagaatacgc ccctctttac gcactcggcg
     9181 acccactcct gatggagcat gagggagtcg tcgttggcta ttctgagata gaagagagca
     9241 ggttcgccgg atacaataag gtcaaagccg ttgtttgcgg cggcggcctt taagccttcg
     9301 gcaaggaggg tgcccttttc tttgaacatc ttgggaccgt cgattgcctt taacttattg
     9361 attgtggcga tggctgcagc gaagggagct gccgacagcc agtagctgcc cgtataggta
     9421 atgccgctga ctacggattt cagatgctcc ttgccgcaga gggcggacat attccagccg
     9481 ttggctatag ccttgcagaa gcagataagg tctgcctcaa agccgaaata gtggtcgctg
     9541 cctgccaggt caagacggaa gccgcagcgt acatcgtcgc agatgagaac tatgccgtgc
     9601 ttgtcgcaaa gctctctgat tctcttccaa tagccttctg ccggcagaac attgtcaaca
     9661 aagttgccgt gcatataagg cgtggagatg aaagcggcga tttcgccctc gttctcggct
     9721 atgacctttt caaaggcgtc agcgtcgttc caggggatat agatattgtt ggcgacatcc
     9781 tcgggcagta cgccgggata gtctatcttc tgagtccagg gagccacgcc gtgatagaag
     9841 ccctttgtaa agactatctt ctttctgtgg gtggcggctc tcgcggtcat tatcgcaagg
     9901 gttgtgacat ctccgccgtt cttggcaaag aaagcccagt ctgcactgtg aacggtgtcc
     9961 acaagcagct ctgccagctc taccatcttg taggagggag aagtggtgca gtttgccttc
    10021 tttaattgct caatggcggc gttgtcaaca tcggggtcgc cgtagcccag cacattgggg
    10081 ccgtaagcgc acatatagtc tataaagcgg ttgccgtcaa catcccagaa gtatgcgccc
    10141 tgagctcttt cggagaacag ggggaacgcg tcaacgggga taaagcagcc ctcggcggga
    10201 ccaagatgtc cgtaaacgcc tgaaggaatg acctctaccg cgcgtttaaa ccattcacgg
    10261 cttttgtcgt acttattaat ctctcttgcc atttccactc ctccttacgc aagatacaga
    10321 ccgacacggt caagtattct gttgaggttg tcaaccatgg aaatcatgcc gcgcatcctg
    10381 atgctgccgt cgccgatgca tgcgactgcg ttgaccttgc cgtcaaacag gtcccttgca
    10441 aggcggatgc ttgaaaactc catgacagcc cttggattct cgggggcctt cttgatggtg
    10501 acgagtcggt gatttctgac cctgacggtt gctgcggggc cgtcggctat ggaaatcatg
    10561 atgtcgccgt caaccatgtg gcttgcggag aacttgccta tctcatcatt gttgcctatc
    10621 tgcgccactg cgacacagat tgtgtagaac ataaggcttg tgctgattct gaagaattcc
    10681 tcgtcctcga gtttttccgg ctcggccctc atgtaggatg agagaaggtc ggtaagcttt
    10741 ttgaactctt taagaaggaa aggaatcttt gtgaatccct ttgtgggaat gggggtaact
    10801 gtgccgtcga taagaccgtt gaacttttcg gggcttgaga aggggatttt aatgtccgcg
    10861 tcgggcaaaa tcgagtcaac cattctgcac ttgccgtttt tgaatgtaat ggtggcaacg
    10921 gggccgccct ttacatcaaa ggcgatggat ataggctttt tgttagtgag cagcgcgctc
    10981 gccttcgggt ccagctcaca tagattttcg agagtaccca ggacagcata catgtttatg
    11041 tacgcaagag tttttgcatc cgtcaaggct attcctcctc tttagtataa atttagccac
    11101 attatttgcg gattcgttgc ggtttcctaa agtcaggaaa atccgaaaat aactgtgggc
    11161 gaacatacga taatattata gcatttactg tacaatctta ccatattaat tttaattttt
    11221 atttaattgt ttttaatttt gcacaattgc aaaaacatct taagtttaat tcgcagccca
    11281 ttccaaaatt tcgcataagc tgtaattgac ctcttaaaga ggaattaata atgaagggct
    11341 cagcataaac gcaacggcaa ttaagactcc cgaaacaagg caaaaaaaac tgtttaccgt
    11401 ttctttaata atctattaca tcggatgcgt cttttccgcc gttttttgct tacagaggtt
    11461 ttaggcaagg caaaatctta ggtgaaatac tgctttttga gctattgtac ggtgaccttg
    11521 atttctcgac cgcttttatg tttgttaaga tgatttttgc cgctgtgaaa ctcggcgcca
    11581 acgcgagaaa actacagcgc aaaataaaaa aagcgggggc ttatcttaaa ttctctgagc
    11641 tttcactctt gattttataa gaacaacaac gggaatagac ctgtttttct tttatatctc
    11701 ccgatttttc ttcacataga cgcgcgtttc ataatcatgc cgctttggga cattgtaata
    11761 ggggt
//
LOCUS       DAONVQ010000059        11551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4315_length_11551_cov_4.695198, whole genome shotgun sequence.
ACCESSION   DAONVQ010000059 DAONVQ010000000
VERSION     DAONVQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4315_length_11551_cov_4.695198"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLD98_05095"
     CDS             <1..185
                     /locus_tag="PLD98_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05659.1"
                     /translation="LNKDKSPDAPTVRVRNVTDTEVVYSSDGKREWAVLRGKNEDLII
                     FKAPDELEINKAEQRL"
     gene            231..1109
                     /locus_tag="PLD98_05100"
     CDS             231..1109
                     /locus_tag="PLD98_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05660.1"
                     /translation="MANCPKCNYKLKLTDIKPNCPSCGTNLMFYGYEERFYREAKYAE
                     MDLAAFRVWIAKVKAAFIGGKLQIARLCACFLPVAALLMPLGKVIVTLPLFEEKIHIS
                     ALGVYNLFTGGGLSLLLELQNSELLGDVAKGLIYSLIAFAAAALCAVGILLTQILCFV
                     NLKRSNIALVVFGSLGIVFAAVSLLLAYRIPDMGSMISSSGSAPGLLCHAAAFGLVLG
                     LNAALIKKGIEVQYKEGDLYRAQMLKRYKRKEITLDDLPFPVFETEEERLEREKKEKE
                     TAEKLKLAQAGGVLNE"
     gene            1102..2469
                     /locus_tag="PLD98_05105"
     CDS             1102..2469
                     /locus_tag="PLD98_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQA05661.1"
                     /translation="MSEQKFVSPEVTRAGKLADKIEASERLQKRLVRVVFILLTLIFI
                     GFLVWGINKVLLTEGSEPMPETDKVYAVGMPESDEKAIALFQKLVSDAADHTRTKLSV
                     KTSLKIDDDSLTVSGDDNELIKKSLIHIKNKVLDGFGEYYPEISTSFGEQFADKLLPI
                     SFIPSDLKSWKAEEDGDNIILSFEFSDAPFNEISEDVKLSLGMDAAEGLTEYAKTHLA
                     PVFQIKNISLISSEPAVSAKADRVSGKLSRVEYNRNYKVELSLLFGGDLKSLGEREIS
                     FDFYANSKYDFTWAGLELADKVMWLQKGKTDVIEARRIADEDVEVQWSSSDESIVSVD
                     SEGYIKGHKTSPEPVTITAAFTYLGKTYTDECLVYVVVPVKDAKLNTRELTLKKGDTY
                     ELSVEIKPKKATVKAVRWFTTDESVAAVDQNGLVTAVGKGECRVYCITENMNLKRSCA
                     IKVEE"
     gene            2479..4074
                     /locus_tag="PLD98_05110"
     CDS             2479..4074
                     /locus_tag="PLD98_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA05662.1"
                     /translation="MAVINESFQEKIFGAEKGSAAKLDKARLDRYRDIAAKMFNTPVL
                     SRREMAFPAIADFASALLRGLDTYRTLYFVNVLKIDMVYVTAILTLIGIYDVMNNPLM
                     GIIYDRTRTRWGKARPYLIFTPLPYFATTALLYSGALFLTNDQTDDPKKIIFVFLTLF
                     AQETFATIYSIPRDNMTSLMSPNPKDRITLGLVTNYAGFFASQTVYGLFLPLQDLNRW
                     GITNVSMPVIFAIFGYITCFFGVLGNMAMGVGCRERIILQPKPAPFTKTIFYILKNKY
                     ALRNFIAEFSTNWWSGGGYSWDVVTQLEIFGGAFRSALFYQPYNTLHPLSITFIPKFQ
                     KFFKNNNKRAYLALRVWDLLTGVGKFATGYFTIENPWLTGATFSFFHALNAVNNAPAT
                     VFEEELRREINDYTEYVTGERPDGTFNLLTGLISKVTSPLNALVTIAVFRWSGYDPTI
                     PMAPWSQGNKVVYRKVYFLFTTFGILPDIIRMLPYFFYDLEGKKREQMYIELNERRAL
                     IANEKANEISEELGAVIEVLEQE"
     gene            4085..5641
                     /locus_tag="PLD98_05115"
     CDS             4085..5641
                     /locus_tag="PLD98_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05663.1"
                     /translation="MDNIFRLGFYESYKPTEIKEKLLADSKANFILWAPSENGKSVKD
                     YTDEVKKSAKEFHEKGLPFVYRCEHTNWAEHGFGPDGHDWYNAGKDAHRMNYPPETVR
                     AISSVPECLGIMHDEMEHAFINRNLSITFNTKLKKRPLFFPLCDTKDVVAAEKYYEDC
                     FRKYAEEFLTNGSPRFMGEHVFPVLYHLFARVGITPCYKQQKESFSNLQAIIAGGAAL
                     QYDTELWACIDMWYRLTYPGHSPNELKHNLLFAYLFGSDYAFVEGSAAFYNKGDESHE
                     YNEYGRTFINFSREYAGKYRGYNIRDYRPRIGIIRLDDSFWGQSDPVAWLPILYGNPN
                     LKPDRRAGEWIRALHLITHGEGSRKSLTWSRIGFYSLKRHRSFCSMNSPAVFDDRVTK
                     DKLTSLELCFLCGYHISEETLKAVAELVKENGLIVVTPKRFAPEHIRAKAKGRQAEIK
                     DGKGSWIITSKHNSRFVKKRVAHLLGKKGEMTFRYKDREIAMRISKDGEAFEVTQIKT
                     LTHSEPVPMR"
     gene            5768..7246
                     /locus_tag="PLD98_05120"
     CDS             5768..7246
                     /locus_tag="PLD98_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05664.1"
                     /translation="MNSDGLGKGMKALSVILGALIGGFIWRCRGESGFGSSWGLYSVG
                     LVLILLIYNFYGSRKGMKYELIPFGAFMTGLGVTGYATVIHQTAGFIYSDLPYQGQVV
                     HAPVDPYSGLIIVLIMGFTLVPLYAFFVGTLFSKKEYKYYHYLIVIAIFFIVQTICKA
                     SVAHYILKLINPEQVNYALLGLKDSGYDYTSPMQAYMDHFLSRRWSQTVPFFENYYMS
                     VEHVSDLIATIAVALYPLIAFKDRVTSLVTVIINAFTSIATTAFTSLMTVMFDTGFLA
                     NVPVPRSLRDGSGWGLWEYATGASVGFITMLVIALLPKELTAKGEIDTEPIFKNKKLS
                     CAVNIIITIFIFGVVPWRAIGIRFAKLLAYEGFLKDYNPTGDILMIAGSIVFGIFFIW
                     FIRKNIIVKNTTPLGADPVRFARIALPAYMGMCCVLYFFTNRAPIFHLPYSEMKSVSS
                     FFYVMTSAQCIEFSLMFITFIAFLAIYIPVRGRLCPTECDKA"
     gene            7309..7569
                     /locus_tag="PLD98_05125"
     CDS             7309..7569
                     /locus_tag="PLD98_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HQA05665.1"
                     /translation="MGLTSLPNIGKELEKQLNEVGITTEEELRKVGSREAWLRILERD
                     PSACLMRLSALEGAIQGIRWHHLSRETKESLKEFYHKHKGSK"
     gene            7582..8343
                     /locus_tag="PLD98_05130"
     CDS             7582..8343
                     /locus_tag="PLD98_05130"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQA05666.1"
                     /translation="MFVSLDDNSLPYPRLCAHRGFNSVAPENSMPAFGAVVALGASEI
                     EFDIWATKDRQLVSIHDPTLDRVSNGSGSVWEYTYDELLRFDFGIKKDERFKGLRIVR
                     FEEILERFARQVIMNIHVKIWDMEFEDNMLREIISLIEKFACEKHIYLMTKSDNMLKA
                     VKKTYPEINICVGHDKNRPFEIVDRAIEIGAHKVQFHKTHFNKELIDKAHENGIHCNI
                     FWSDDPDEAKYLLNMGIDTILTNDYQRISFATGLK"
     gene            complement(8411..10171)
                     /locus_tag="PLD98_05135"
     CDS             complement(8411..10171)
                     /locus_tag="PLD98_05135"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05667.1"
                     /translation="MTVAKKVLAVLLSIVMAFSVFTMATSADSGFEPIDKNMGKILDI
                     FEAVENLVTKIVNFFKRIFGLVTDRNFYDVEVVHNEHGTVIPITGYSILEKTSFICEI
                     FPEEGYYVKAIMFGDRDITDRLVGTHLVTPDLTSNITLTIVFDRISYTISVAVSGMGS
                     VSPSPNASVLYGENASFTVTPSTGYELVSVVYNGIDVTSQLVDGVFTTPAVFSNGTLV
                     ATFEESVPVNVVYVAADGAGDGSSWDSAFGSIQAAIDAAAATEPKGVVWVKAGTYTVS
                     STIVCKPGVNVYGGFAGTEERASDRQRADLDGNGLVEPWEFANATVIRPSAANVIALR
                     IPSTITADVIVDGFTFTNGHLAAGTNLVQVYGNAAAGGRGILKNSSIVDSKNTGAGGR
                     TRQTTGIVFVDGGTVDSCYLANNDSSGSYGGGAISTYYDYSEASPIYITNCKFVNNKA
                     VYGGGVYFRGNTYYLTNSIFIGNEAGGASDSKGGAVFFTGGSNPAAGRVDNNTFIGNT
                     APEFGGLYLKADATATRNLFLNNSQSGTVQYGGDGTFTDNAASFVDNLAAAGINSYYF
                     VSADSDFYNYGAVPVGTPAN"
     gene            complement(10937..11245)
                     /locus_tag="PLD98_05140"
     CDS             complement(10937..11245)
                     /locus_tag="PLD98_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HQA05668.1"
                     /translation="MENVESTASEKKINLNIITPRGVKFTEKADMLVMRCIDGDLGVL
                     PGHEKLSAVLGDGILRVYSGGRLTKIALFGGTAEIEDNSVNIYTTIAQRPEEIDRERA
                     "
     gene            complement(11247..>11551)
                     /locus_tag="PLD98_05145"
     CDS             complement(11247..>11551)
                     /locus_tag="PLD98_05145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081232.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQA05669.1"
                     /translation="ILQRYKDLQDIVAILGMDELSEKDKLIVNRARKVQRFLSQPFYV
                     GEKFTSIPGRYVPIEETVRGFKEIIDGLHDDIPEGYFLNAGTIDEVVERYNREKGA"
BASE COUNT         3038 a         2784 c         2781 g         2948 t
ORIGIN      
        1 atttaaacaa ggacaaaagc cccgacgcgc ctaccgtaag ggtgagaaat gtcaccgata
       61 ccgaagtcgt ttactcatcc gacggcaaaa gagagtgggc tgttttaaga ggcaaaaacg
      121 aggaccttat tatattcaaa gcgcccgatg aattggagat aaacaaagct gagcaaaggc
      181 tgtaatgctc tcgcattttg acaaactgtc tttaataaag gagttttgtt atggctaatt
      241 gcccaaagtg caattataag ctgaaactga cggacataaa gccaaactgc ccgagctgcg
      301 gcacgaatct tatgttctac ggctatgagg aaaggttcta cagagaggca aagtacgccg
      361 aaatggacct tgccgctttc agggtatgga ttgccaaggt gaaggctgcg ttcataggcg
      421 gcaagctgca aattgcccgc ctgtgcgcct gtttcctgcc cgtcgccgcg ctgcttatgc
      481 ccctcggaaa ggtgattgtc actcttcctt tattcgagga gaaaatacat atcagcgccc
      541 tgggggttta caaccttttc accggcgggg gactcagtct cctgctggag cttcaaaact
      601 ccgagcttct gggagatgtg gcaaaggggc tgatttactc cctaatcgcc tttgccgcgg
      661 ctgcgctttg cgctgtgggt attcttctca cgcagattct ctgctttgtt aatctaaagc
      721 ggtctaatat cgcgcttgtc gttttcggct cgctcggcat agtctttgcg gcagtgtccc
      781 ttttacttgc gtatagaata cccgatatgg gcagcatgat ttcctcaagc ggcagcgctc
      841 cggggctgtt gtgccatgcc gccgcattcg ggttagtttt aggtctcaac gccgctctta
      901 taaaaaaggg catagaggtt cagtacaaag agggcgactt ataccgagct caaatgctga
      961 aacgctataa gagaaaggaa atcacccttg atgatttgcc cttccccgta tttgaaaccg
     1021 aggaagagag attggagcga gaaaagaagg agaaggaaac agccgaaaag ctgaaactcg
     1081 cacaggcagg gggcgtattg aatgagtgag caaaaattcg tatcacccga agttacccga
     1141 gcgggcaaac tcgccgacaa gatagaggca agcgaacggc tgcaaaagcg gcttgtccgc
     1201 gttgtgttca ttctgctcac gctgatattt atcggatttc tggtctgggg cataaacaag
     1261 gtgcttttaa ccgaaggcag cgagccaatg cccgaaacgg acaaagttta cgccgtgggt
     1321 atgcccgaga gcgatgagaa ggctatagcg cttttccaga aacttgtaag cgacgctgcc
     1381 gaccatacaa ggacaaagct cagcgttaaa acgagcctga aaattgatga cgacagtctc
     1441 accgtcagcg gcgatgacaa tgagctaata aagaagtcgc ttatacatat taaaaataaa
     1501 gttctggatg gcttcggtga atactaccca gagatttcca catccttcgg cgagcagttt
     1561 gcggacaaac tcctgcccat ctcctttata ccctcagatt taaagtcatg gaaggcggag
     1621 gaggacggcg acaatataat tctttccttt gagttctcgg acgcgccatt taatgaaata
     1681 agcgaagatg taaagctctc actgggaatg gacgccgcgg agggtttaac agagtatgca
     1741 aaaactcatc ttgcccccgt tttccaaatt aagaacatca gccttataag ctcagaacct
     1801 gccgtcagcg caaaagcgga cagagtaagc ggcaagctca gccgcgttga atataaccgc
     1861 aattacaaag tagagctgag tcttcttttc ggcggcgacc ttaaaagcct tggcgaaagg
     1921 gagattagct tcgatttcta tgccaattcc aaatatgact ttacatgggc gggtctggag
     1981 cttgccgata aggttatgtg gctacaaaag ggcaaaaccg atgtaatcga agcccgcaga
     2041 atcgccgacg aggatgtgga ggttcagtgg agctcatcgg acgaaagcat tgtttcagtg
     2101 gactccgagg gctatatcaa gggtcacaaa acaagcccgg aacccgtcac cattacagcg
     2161 gcctttactt atctcggcaa aacatatacc gatgaatgcc ttgtctatgt ggtggttccc
     2221 gtaaaagacg cgaaattaaa cacaagggag ctgactttaa agaagggcga tacctatgag
     2281 ctgtcagtag agattaagcc caaaaaagct acggtcaaag cggtgagatg gtttacaacg
     2341 gacgagagcg tcgccgctgt tgaccaaaac ggacttgtaa ccgctgtggg taaaggagaa
     2401 tgcagggtct actgcataac ggagaatatg aacctaaagc gttcctgcgc aattaaggtg
     2461 gaagaatagg gtgatttgat ggcggtaatc aacgagagtt tccaggaaaa gattttcggc
     2521 gcggaaaaag gctccgcggc aaagctggat aaggcaaggc tggacagata ccgcgacatc
     2581 gcggctaaga tgttcaacac gcccgtttta agccgcaggg aaatggcatt ccccgcaatt
     2641 gccgactttg ccagcgcgct tttaaggggt cttgatacat accgcaccct ttactttgtg
     2701 aatgtgctga aaatagatat ggtctatgtt acggccattc tgacccttat cggtatatac
     2761 gatgttatga acaaccccct tatgggcatt atttatgacc gtacccgaac tcgctggggc
     2821 aaggcaagac cctatctgat tttcacgccc ctcccgtact ttgccaccac cgcgctttta
     2881 tacagcggcg cgctgtttct gactaacgac caaacggacg acccgaaaaa gattatcttc
     2941 gtctttctga ccctctttgc tcaggagacc ttcgccacaa tctactcaat cccccgcgac
     3001 aacatgacga gccttatgtc acccaaccca aaggacagga ttacattagg tcttgttaca
     3061 aactacgccg gctttttcgc ttcgcaaacg gtgtacggcc tgttccttcc cttgcaggat
     3121 ttgaaccgct ggggcatcac caatgtttct atgcccgtaa tcttcgcgat attcggctat
     3181 atcacctgtt tcttcggtgt tctgggcaat atggcgatgg gcgtcggctg cagagagaga
     3241 atcatcctcc agccaaaacc cgctccgttt acaaagacga ttttctatat cctaaagaac
     3301 aaatacgccc ttagaaactt catcgccgag ttttccacca actggtggag cggcggcggc
     3361 tattcgtggg atgttgtaac ccagcttgag attttcggag gagctttcag aagcgctctt
     3421 ttctatcagc cctacaatac gctccacccg ctttccatta ccttcatccc caaattccag
     3481 aagttcttta agaacaacaa caaaagggca tatctcgcgc tcagggtttg ggatttgctt
     3541 acgggcgtcg gaaagttcgc gacgggctac tttactatcg agaatccgtg gctcacgggc
     3601 gccacattca gcttcttcca tgcccttaac gccgtcaaca acgctcccgc aaccgttttt
     3661 gaagaggagc ttagacgaga gattaacgac tacaccgaat atgtaaccgg cgagcggccc
     3721 gacggcacct tcaatctgct tacgggactt atctcaaaag tcacctctcc tttaaatgct
     3781 cttgtgacaa tagccgtttt cagatggtcc ggctacgacc ccacaatccc catggctccc
     3841 tggtcccagg gcaacaaggt ggtttacaga aaggtttact tcctctttac aaccttcgga
     3901 attcttcccg atattatcag aatgctgccc tacttcttct acgaccttga gggcaaaaag
     3961 agagagcaga tgtatattga gctgaacgag cgccgcgccc tcatcgcaaa tgagaaggcg
     4021 aacgaaatct ccgaggaatt gggcgcagtg attgaagtgc ttgagcagga ataaggtgat
     4081 aagaatggac aatatattca gactcggctt ttatgagagc tacaaaccta cagagattaa
     4141 agagaaattg cttgccgact ccaaagcgaa cttcatctta tgggcgccca gcgaaaacgg
     4201 caaaagcgta aaagactata cggatgaagt aaaaaagtca gcaaaggaat tccacgaaaa
     4261 agggcttccc tttgtgtacc gctgcgagca caccaactgg gctgagcacg gctttggtcc
     4321 tgacggtcac gactggtaca acgcgggcaa ggacgcccac agaatgaact acccgcccga
     4381 aacggtgcgg gctataagct ctgtccctga gtgtctcggc atcatgcacg atgagatgga
     4441 gcacgccttt ataaatcgta acctttctat aacctttaac acaaagctta aaaagcgccc
     4501 ccttttcttc cccctttgtg acacaaaaga tgtagtcgcc gccgaaaagt actatgagga
     4561 ctgtttcaga aagtacgcag aggagtttct cacaaacggt tcaccccgct ttatgggcga
     4621 gcatgttttc cccgttctct atcacctttt cgcaagggtg ggcataaccc cctgctacaa
     4681 gcagcaaaag gagagctttt caaaccttca ggctataatc gccgggggag cggctctgca
     4741 gtatgacaca gaactttggg cctgcattga tatgtggtat cgccttacat accccggaca
     4801 ttctcccaat gagctgaagc ataaccttct tttcgcttac cttttcggca gcgactatgc
     4861 ctttgtggag ggcagcgcgg ccttctataa taaaggcgac gaaagccacg agtacaacga
     4921 gtacggcagg acattcataa acttctcaag ggaatacgcg ggcaagtaca ggggctacaa
     4981 catccgcgac taccgccccc gcataggcat tataaggctt gacgattcct tctggggtca
     5041 gagcgaccct gtggcatggc tgccgattct ttacggaaac ccgaatttaa agcccgacag
     5101 aagggcgggg gagtggattc gcgccctgca ccttataaca cacggcgaag gctcaagaaa
     5161 gagccttaca tggtcaagaa tcggctttta cagccttaag cgacaccgct ccttctgctc
     5221 aatgaattcc ccagcggtct ttgacgatag ggtcacaaag gataaactca catctttaga
     5281 gctttgcttc ctctgcggat atcatataag cgaggaaact ttaaaggctg tcgccgagct
     5341 ggtgaaagag aacggtctta tcgtcgttac ccccaaaaga tttgcccccg agcacattag
     5401 ggcaaaggct aaaggcagac aggcggaaat taaggacggc aagggctctt ggattataac
     5461 aagcaagcat aactcccgct ttgttaaaaa gagagtcgcc catcttcttg gcaaaaaggg
     5521 cgagatgacc ttccgctaca aggaccgcga aatcgccatg cgcatctcaa aagacggcga
     5581 ggcatttgaa gtaactcaaa taaaaacgct cacccactca gagccggtac ctatgagata
     5641 aaactgtttg aaaaacttaa ttaaaatatt ccccttgagc aaaggggaat atgtttgtat
     5701 tatttaagca ttagatttaa aaggaaaatt tgtatttacg cttaatattt tgaaagaggt
     5761 gtgccaattg aattctgacg gtcttggaaa gggaatgaag gcgctgtccg ttatcctcgg
     5821 cgcgctgata ggcggcttta tctggcgctg ccgcggtgag agcggctttg gctcgtcatg
     5881 gggcctttac agcgttggtt tggttctgat tcttttaatc tacaacttct acggcagccg
     5941 caagggaatg aagtacgagc ttatcccatt cggcgcgttt atgacgggcc tcggcgttac
     6001 gggctacgcc acagttattc atcagactgc gggctttatt tacagcgacc tgccctatca
     6061 gggtcaggtt gtccacgccc ccgttgaccc ttacagcgga cttatcatcg tgctgataat
     6121 gggctttacg ctggttcccc tttacgcctt ctttgtcggc acccttttct caaaaaagga
     6181 gtataagtac tatcactacc ttattgtgat agccatcttc ttcatcgttc agacaatatg
     6241 caaggcgagc gtcgcccatt acatattaaa gcttataaat cccgagcagg tgaactacgc
     6301 tttgcttggt ctcaaagaca gcggctacga ctacacaagc cccatgcagg cttatatgga
     6361 ccacttcctc agccgccgct ggtcccagac cgtccccttc tttgagaatt actacatgag
     6421 cgttgagcat gtttcagacc tcatcgccac aattgccgta gctctctatc cgctgattgc
     6481 ctttaaggat agggttacga gccttgtaac ggtgattatc aacgccttta cctcaatcgc
     6541 caccacagct tttacctctc ttatgaccgt gatgttcgat acgggttttt tggcaaatgt
     6601 ccctgttccc agaagcctta gagacggcag cggctgggga ctttgggagt acgctacggg
     6661 cgcctctgtg ggctttatca ccatgcttgt gattgctctt ttgccgaaag agcttaccgc
     6721 aaagggagaa atcgacacag agcctatatt caaaaacaag aaactaagct gcgccgttaa
     6781 tataattatc accatcttta ttttcggcgt cgtaccgtgg agggctatcg gtatccgctt
     6841 tgcaaagctc cttgcgtacg aaggcttcct caaagactat aacccgacgg gcgatattct
     6901 tatgattgct ggctctattg tgttcggtat attcttcatt tggtttatta ggaagaacat
     6961 tatcgtaaaa aacacaactc ctctcggagc agatcccgtc cgctttgcga gaatcgctct
     7021 gcccgcgtat atgggaatgt gctgcgttct ctatttcttt accaaccgcg cgcccatatt
     7081 ccacctgcct tactccgaga tgaagagcgt ttcttccttc ttctatgtaa tgacaagcgc
     7141 tcagtgcatt gagttttcgc ttatgttcat aacctttatc gccttcctcg caatctatat
     7201 ccccgttcgc ggaaggcttt gccccactga gtgtgacaag gcttaaataa ccttaaaagc
     7261 actatgggca aaagctcata gtgccttgta tttaaaggag atgaaaaaat ggggttgaca
     7321 tcgcttccta acatcggaaa agagctcgaa aagcagctta atgaagttgg aattaccact
     7381 gaggaagagc tgagaaaggt aggcagcaga gaagcgtggc tgaggatttt ggaaagggac
     7441 ccctccgcct gtcttatgcg cctttccgcc cttgaggggg caattcaggg tataagatgg
     7501 catcacctca gcagggaaac aaaggaatct cttaaagagt tttaccataa gcacaaaggt
     7561 tcaaaataaa ggagtttaaa tatgtttgtt tcccttgacg acaacagttt gccctatccc
     7621 cgcctttgcg cccacagggg ctttaactcg gtagctcccg agaacagcat gcccgccttc
     7681 ggggcagtgg tggctctggg cgcctctgaa atcgaatttg acatctgggc tacgaaggac
     7741 aggcagctcg tatccatcca cgacccaact cttgacagag tgtcaaacgg cagcggcagc
     7801 gtgtgggaat atacttatga cgagcttttg cggtttgatt tcggcattaa aaaggacgag
     7861 agattcaaag gacttagaat cgtaagattt gaggagatac tcgaaagatt tgcccgccag
     7921 gtcattatga atatccatgt gaagatttgg gatatggaat ttgaggacaa tatgctcaga
     7981 gaaattattt ccctcataga aaaatttgct tgcgaaaagc acatttactt aatgaccaaa
     8041 agcgataata tgcttaaagc cgtgaagaaa acctatcccg aaatcaatat ctgcgtgggt
     8101 cacgacaaaa accgcccctt tgagattgtg gacagggcaa ttgaaatcgg cgctcataag
     8161 gtgcagttcc acaaaaccca ttttaataaa gagctgattg acaaggcgca cgaaaacggc
     8221 atacactgca atatcttctg gtccgacgac cccgacgaag cgaaatatct ccttaatatg
     8281 ggcatagata caatcctcac aaacgactac cagcgcatct cctttgccac cggacttaaa
     8341 taaaccgaaa atttacgcct taaataaaaa gctgcaagca tttctgcttg cagcttttgt
     8401 ttggtttagt ttagtttgcg ggggttccta cgggaacagc gccgtagttg tagaagtcgc
     8461 tgtctgcgct tacaaagtag taggagttga ttcctgcagc ggcaagattg tcaacgaagg
     8521 atgcggcgtt gtccgtaaat gtgccgtcgc cgccgtactg aacagtaccg ctctgtgaat
     8581 tgttaaggaa gaggtttctg gttgcggttg cgtcagcttt cagatacaga ccgccgaatt
     8641 cgggagcggt gttgccgatg aaggtgttgt tgtcaacacg acctgccgcg gggttggagc
     8701 cgcctgtgaa gaatactgcg ccgccctttg aatccgatgc gccgccggct tcgttgccga
     8761 tgaagatgct gttggtcagg tagtaggtgt tgccgcggaa gtagactccg ccgccgtaaa
     8821 cagctttgtt gttaacaaac ttgcagtttg tgatgtaaat cgggcttgcc tcgctgtaat
     8881 cataatatgt gcttatagcg ccgccgccgt atgagccgga agagtcgttg ttcgcaaggt
     8941 agcagctgtc aacagtgccg ccgtcaacaa atacaatgcc cgttgtctgg cgggttctgc
     9001 cgcctgcgcc tgtgttcttg gagtcaacga tggaggaatt cttcagtatt cctctgccgc
     9061 ccgcggcagc gttgccgtaa acctgaacaa ggttcgtgcc tgctgcaagg tggccgttgg
     9121 tgaatgtaaa gccgtcaacg attacatcag cagtaatggt tgaggggatt cttaaagcta
     9181 tgacattggc tgcggagggt ctgattaccg ttgcgtttgc gaactcccaa ggctcaacaa
     9241 gtccgttgcc gtcaaggtcc gcgcgctgac ggtcgcttgc gcgttcctct gttcctgcaa
     9301 agccgccgta aacattaacg ccgggcttgc aaactattgt ggaggatact gtatatgtgc
     9361 ctgcctttac ccaaacaacg cccttgggtt ctgttgcggc tgccgcgtct atagcggcct
     9421 gaatgctgcc gaaagcagaa tcccatgagg agccgtcgcc tgcgccgtcg gctgcgacat
     9481 aaacaacatt tacgggaaca gactcttcaa atgtcgcgac aagtgtgccg ttggagaaga
     9541 ccgcgggagt tgtgaaaacg ccgtcaacaa gctgtgatgt tacatctatg ccgttgtaaa
     9601 ctacggaaac aagctcatag cctgtggagg gggttaccgt aaagcttgcg ttctcgccgt
     9661 aaagaacgga tgcgttcggg ctgggtgata cgctgcccat gccgcttacg gcaacactga
     9721 ttgtatagga gattctgtca aatacgattg tcagtgtgat gttcgaggta aggtcggggg
     9781 ttacgagatg ggtgccgaca agcctgtcgg taatatcccg gtccccgaac ataattgctt
     9841 ttacatagta gccctcttcg gggaaaatct cgcagataaa gctggtcttt tcaagtatgg
     9901 aatatccggt tatcgggatg acggtaccat gctcattatg taccacttcc acatcgtaga
     9961 agtttctgtc cgtcaccaaa ccgaaaatcc tcttgaagaa gttgacgatt ttcgttacta
    10021 agttttcaac cgcctcgaaa atgtcaagga ttttgcccat gtttttgtcg atgggctcaa
    10081 aaccgctgtc ggccgatgtt gccattgtaa agactgagaa agccataaca atgctaagca
    10141 ggacggccag cacttttttt gcaactgtca taatctctac ctccgttttt agttttttct
    10201 gccatttctt agacttttaa atgtccgtcg aaacaactcc tttccgaatt tgcgaactat
    10261 aagcaaattc aggtcgaatt acgatttttt tgttaaaaag gaaaaatagt gtacttcatt
    10321 ataggtatcg caatattgca ttaatatcca aaaattgtta attttctgtg aataggactt
    10381 cgaaaaacgg attaaaagaa aaaaaaggaa gaaaactgcg acaaaaattg cttttaaaat
    10441 ttaatgaatg ataacaaaaa atacacgaat atttagtaga aatttttatt attattgaca
    10501 aaaatatggc acatctcgtc gttttttgcg gaaaaaactt tcacgggcaa aagcaaaaaa
    10561 ttgtaaataa aattttaatg tttctgtaag gcattatgaa aaaaatcctg aataaagctg
    10621 tgacgcaaat tgtagatttt gattacctgc tgtttccgta agtttaatga atcatatttt
    10681 ggcgcaaata aaaaagcagg cgattttgcc tgctttttgg tgttttttaa cgcatttttg
    10741 acggcggaat gaaaagtaag cggtattatt cgtcttcctc gtcattttct tccgtgtcat
    10801 cataaaaatt tgattcatca tgatgggagc tgacctcaag ccgcacaagg gagcgttgga
    10861 gaagtacctg cagttgtctg ctctgctttt cttccgatga ctccttgagg gattcatccg
    10921 catcatgtct gtctctttag gcccgctctc tgtctatttc ctcagggcgc tgggctatgg
    10981 tggtgtatat gttaacgcta ttgtcctcaa tttcagccgt gccgccgaaa agcgcgattt
    11041 ttgtgagcct gccgccgctg tagactctca gtatcccgtc gcccaaaaca gcagacagct
    11101 tttcgtgccc cggcagaacg ccaaggtcgc cgtcaatgca gcgcataacg agcatatccg
    11161 ccttttcggt aaactttacg cccctcggcg tgattatgtt aagatttatt ttcttctccg
    11221 aggctgtgct ctctacattt tccatatcat gcaccctttt cgcggttgta tctctccaca
    11281 acctcgtcta tggtgccagc attaaggaaa tagccttcgg ggatatcgtc gtgaagaccg
    11341 tcgattatct ccttaaagcc cctgaccgtt tcctcgatgg gcacatatct gccgggtatg
    11401 gaggtaaact tttcgcccac atagaagggc tgggacaaaa agcgctgcac ctttcttgct
    11461 ctgttgacga tgagcttatc cttttccgat agctcatcca tgcccaaaat ggcgactatg
    11521 tcctgcaaat ccttgtacct ctgaagtatg c
//
LOCUS       DAONVQ010000060        11301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4438_length_11301_cov_4.091499, whole genome shotgun sequence.
ACCESSION   DAONVQ010000060 DAONVQ010000000
VERSION     DAONVQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11301
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4438_length_11301_cov_4.091499"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PLD98_05150"
     CDS             <1..1198
                     /locus_tag="PLD98_05150"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQA05670.1"
                     /translation="DVQDVAESAGQVLAGTLDIEEFQAYEDEVCPGCGSCQGMFTANS
                     MACMTEVLGISLPGTGTVPAVNAKRYHMAKKAGQIAVELAKYDFRPSEFLTRESFINA
                     ITLDMLLGCSTNTALHLPALARSIGVDISLDDFDEISRKTPHIVKLSPSGKHLMQDLD
                     VAGGVSAVIKQAIEAGLINGEQMTVTGKTLAENVENAEVLDPEVIHPISNPYSEEGGL
                     MVLKGNLAPLGAVIKSAGVLPEMFEHEGPARVFDSERHAMSALAMRRIKPGDVMVIRY
                     EGPKGGPGMPEMLTLTALLAGQGLDSSVALVTDGRFSGASRGGVIGHVAPEAALGGPI
                     AFVQEGDIIRLSLSKRTIEVLVDDEVLEERKKNWIPPAPKIKTGWLARYAKLVGPVSG
                     GAMLEE"
     gene            complement(1251..1466)
                     /locus_tag="PLD98_05155"
     CDS             complement(1251..1466)
                     /locus_tag="PLD98_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HQA05671.1"
                     /translation="MLDRISLVLVIIGALNWGAIGLFQFDVVAWLFGGQAAIISRIIY
                     TIVALAGIWCISLLFRESEVLASETDE"
     gene            1653..2798
                     /locus_tag="PLD98_05160"
     CDS             1653..2798
                     /locus_tag="PLD98_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA05672.1"
                     /translation="MPADKVLRFNRDGKFTILTVADMHLKDNGEKGSADTLKLLEASL
                     IKFDPDLTVLLGDNTSGWKGSSFEGVERVISSITEIFDRHKKLFAVVFGNHDHETGVS
                     NKEQAELFQKSEYCLLAEGDCEVGCGNYNLPILRSDSDKMAFNLWMIDSRDYYEDENG
                     NRSYAFVSDEQIAWYEKKSEELKKKNGGMPVYSLVFQHITVPEQYRLLKAVHPLTPFA
                     VKGHGAWSDRRFVIARPKYTKGALGEGPCPPDINNGQFMSWLKTGDVIGAVFGHDHIN
                     DFVGDVDGIKLIQTRGAGFTAYGDGIDRGVRLITLYENEPKKLKTDTYYYTEIVGSIP
                     TTLPLHEMLPLRIKKKLICAGSAALLTASLLAAAKAVNAIKKCKHRV"
     gene            2804..3373
                     /locus_tag="PLD98_05165"
     CDS             2804..3373
                     /locus_tag="PLD98_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HQA05673.1"
                     /translation="MNVYDFDNTIYDGESSLALFWFYIKKMPHLVKYYPKVLKAMYNY
                     KKGNITIEDALQKYIPMIEPYVLKVIDTCDRDAKEFWDSHMHKIKPFYKEIQQDDDVI
                     ITASPEIFMREICNRLGVKEVIGTTFNHETGKIERFCYRKNKVKAFKERFGDAPIDKF
                     YTDSINDAPLIEIAKEAYLVKGNKIQRIK"
     gene            3388..5235
                     /locus_tag="PLD98_05170"
     CDS             3388..5235
                     /locus_tag="PLD98_05170"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HQA05674.1"
                     /translation="MRNKNVSYKNIDITGGFWKDRQDLNKIVTIYAVRDRFKDTGRFD
                     AFKCDWTEGKPKKPHFFWDSDVAKWMESAAYILSKERNEELEKEVDALVDLIEKNQDE
                     NGYFNIYFTVVEPDERFTIRDAHELYCAGHLIEAAVAYYEATGKDKFLKLMKKYADYI
                     EKVFKIEDSAEFATPGHEEIELALVRLYRCTGEEKYLNLSKHFIDSRGARGDKEELMI
                     YNQSHMPVREQRTAEGHSVRACYLYSGMADIAAEFDDEELKVALNSLFDNIVNRRMYV
                     TGGIGSSHHGDAFTIDYDLPNPNAYAETCAAISLAMFAGRMNEIRPNGKYGDIVEKVI
                     YNGFLSGLSLDGKSFFYENPLEIVRADRTRNVSWPAKERFPLTKRKEVFDCSCCPPNV
                     TRFIASIGGYVYTVGSDENTLYVNQYMESVSNLKLGDADVEITQKTNYPWDGKVNITA
                     KGMKGKTLALRIPAWSEEFTVEINGKTADIKPTDGFARIEVDADEISAALRLDVSPFF
                     VECNPKVNANAGRVALQAGPVVYCLEEIDNGPNLRDISISREPDFKVKWFDELGVQII
                     EAKAFRTKPFDELYRRAQFEKEECKAVFIPYFAFANREECDMLVWTKVE"
     gene            complement(5277..5915)
                     /locus_tag="PLD98_05175"
     CDS             complement(5277..5915)
                     /locus_tag="PLD98_05175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQA05675.1"
                     /translation="MKQYFKESAVVLFQGDSITDCGRDRNDITSLGNGYPQIVANIYR
                     TMFPKRNITFINKGISGNRVRDLLARYDEDFLAIKPDFISILIGINDVWRAFDRNDPC
                     PLERFEQEYDLLLTKIRTDMPDTKIMVISPFVLHSLPDRETWHPDLDPKIEVCEKLAA
                     KHNCIYLPMQSIFNKAVKRDYTPKELAEDGVHPTHFGHAYIAAEYLKKLRIL"
     gene            complement(5934..7109)
                     /gene="nagA"
                     /locus_tag="PLD98_05180"
     CDS             complement(5934..7109)
                     /gene="nagA"
                     /locus_tag="PLD98_05180"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HQA05676.1"
                     /translation="MSTTVYKNANIVLYDRIRKGNLIVKDGKIAEIVDRFDIKDAETV
                     DVKGLYLAPGFVDIHVHGGGGYSAMSGNPEDIKNMCKAHAVRGTTSILPTTLAAPMPK
                     IVAAVEAIKKAAEDYTFSNILGAHLEGPYLSLEMKGAQSPENIINPVDVSPTELLDLW
                     KGIRMVGAAPEIPGGMELGKEISKRGIVASVAHSNATYEIVDKALDYGYSDITHIYSA
                     CSSVIKVNLFRVAGVVEAGLSLDGYTTQFIADLRHLPVGLIKLIYKAKGPDRAYAITD
                     GLEFSASDMKEGEIYTQENDLAVVYEDGVMKLADRSSLAGSVATCADLVRNMYKKCDI
                     SLVDSVKMASTTPARVVGFGHRKGLIKANYDADLVIFDDDVNIKAVVTNGNIIVNKI"
     gene            7352..8638
                     /locus_tag="PLD98_05185"
     CDS             7352..8638
                     /locus_tag="PLD98_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA05677.1"
                     /translation="MSEKTVRIGAVGISGRGSSMLRLMMRFKGVVCPAVCDVYEDRMN
                     DGIEIVKNSPHYSGYEVKGYLDYKEMFEKEQLDAVLICTTWITHARIAVDAMRAGFHV
                     GLEVGGAASEEECWQMVRASEATGKFCMLLENCCYGQAEMAVFNMVKLGLFGEIVYLT
                     GGYQHDLRDEIGYGREIRHGRLFNFMHRNGELYPTHQLGPIAKLIGINRGNRFLSLVS
                     MSTKARGLNEFLKREKGEGYDIANYPFNQGDVTTTIIKCAGGENITLIHDCTLPRPYS
                     RNARVQGTKGIWMEDGNQIYIEGRSPFHKWEDFDPYMHEFAHPLWRIHAKNAAKYGHG
                     GMDYLIMAAFIESAMHNLKPPIDVYDTASWMAITYLSEQSVAMGGMPVPVPDFTNGRW
                     IDREPYRRGIFCLEEVCDEFFTDEDMNIDEEILKDR"
     gene            8654..9592
                     /locus_tag="PLD98_05190"
     CDS             8654..9592
                     /locus_tag="PLD98_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HQA05678.1"
                     /translation="MKYYIGIDGGGTKTQYALFDENKNLISSVFTGGSNHENLEGSFS
                     EAADIIHEGITNLLKENGLEYKDIEHTLMGLAGIDHPYQHDAMVDELEERGYSRFSIY
                     NDGFIVTKAGTDKKAAIGYNCGTGTCCNSIDSRGNMLQIGGFGDLSGDAGNGSWIAIR
                     AFRLVYDEICLARHKSLITKLFTEKFGVKPERDEFLATIALLMGLKQEEYTRGLIDIF
                     FDAANAGDEVVLESIEEMADRGADFIAAHTKLQQFDDEVVDVVLSGSIHTKLPADIYI
                     ERLMEKARAKSLKKLNFIKLTGPPVMGCINWMLEDK"
     gene            9618..10898
                     /locus_tag="PLD98_05195"
     CDS             9618..10898
                     /locus_tag="PLD98_05195"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HQA05679.1"
                     /translation="MKEIVVAVIGSGSTYCPELVDGFIKAKDRLGLKSLRLMDIDERK
                     LNIVGDLCIRMLRNAGIDCEVIKTDSLDTALKGADFVVTQIRVGKLPARHLDETIPLK
                     YDLIGQETTGIGGFFKALRTIPVMKEIADKVKELCPDAWLINFTNPSGIISEFLQNHT
                     DIKSMGLCNVPINMIDDVKKAVGQDADVTYLGLNHLSWITSVKKDGKEYIDRLFDEGF
                     APEVMANIHDDGFDISCLKAARGIPSSYLQYYYNRAAKLEYLKDSEQSRAEVCMEIEE
                     ELLKMYADPDLKEKPALLDLRGGHKYSLAAVSLIDSIANDTNDIHVVNIKNNGTLHFM
                     EDDDVVEIAAAIGKDGAKPVKLERLENEHIIGLMRIVKAYERLAVKAGMDGDDDAALN
                     ALMLHPLVGDFEKAKACYEEMKEAHAAYLPQFKK"
     gene            10979..11260
                     /locus_tag="PLD98_05200"
     CDS             10979..11260
                     /locus_tag="PLD98_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQA05680.1"
                     /translation="MKVQGYSLKVKKTRQKIRNFYTAAQGLPSDAITAAAFDGKGVLW
                     VGTDKGLAKFDGKKFAKFDVKAGNGNISMLHVDKEGALYVGTENKLVKV"
BASE COUNT         3172 a         2726 c         2770 g         2633 t
ORIGIN      
        1 ggatgttcag gatgtcgccg aatccgcagg tcaggtgctt gcgggcaccc ttgacataga
       61 ggagttccag gcatacgagg atgaggtctg ccccggctgc ggctcatgcc agggtatgtt
      121 caccgcaaac tccatggcat gcatgaccga ggttctcggc atctccctgc ccggtacggg
      181 cacggtcccc gcagtaaacg caaagcgtta tcatatggct aaaaaggcgg gtcagattgc
      241 cgttgagctt gcgaagtacg acttcagacc ctccgaattc ttaacaaggg agtctttcat
      301 aaacgcaatc accctcgata tgctcctcgg ctgctcaacc aatacggcgc tgcatctgcc
      361 cgcgctggca cgctcaatcg gagttgatat aagtcttgac gactttgacg aaatcagccg
      421 caaaactccc catatcgtaa agctgtcacc gtcgggcaag catcttatgc aggaccttga
      481 tgttgccggc ggcgtgtctg ctgtaattaa gcaggcgata gaagctggac ttattaacgg
      541 cgagcagatg accgtcacgg gcaagacact cgccgaaaat gttgagaacg cggaggttct
      601 ggaccccgag gtcattcacc ccatctcgaa cccctattcc gaagagggcg gactcatggt
      661 cctcaagggc aatttagccc ccttgggagc cgttatcaag tccgcgggcg ttctgcctga
      721 aatgtttgag cacgaaggcc ccgcaagggt attcgattcc gagcgccacg cgatgtccgc
      781 gctcgctatg aggagaatca agcccggcga tgtaatggtc ataaggtacg aaggacccaa
      841 gggcggaccc ggtatgcccg aaatgctgac ccttacagct ctgcttgccg gtcagggact
      901 tgacagcagc gttgcccttg tcactgacgg cagattctca ggcgcttcaa ggggcggcgt
      961 cataggtcat gttgctcccg aggctgctct gggcggtccg atcgcctttg tgcaagaagg
     1021 agatatcatt aggctcagcc tcagcaagcg cactatcgag gtgcttgttg acgacgaagt
     1081 tctggaggag agaaagaaga actggattcc tcccgcaccc aaaatcaaaa ccggctggct
     1141 tgcccgctac gcaaagcttg tcggcccagt ttcaggcgga gcaatgcttg aagaataaac
     1201 aaatcggggc ggcaaaaagt cgccccgttt aatattttga cagtttaatg ttattcatca
     1261 gtttccgatg caagaacctc gctctctctg aacagcagag aaatgcacca gataccggca
     1321 agagctacta ttgtatatat aattctgcta atgatagcag cttgtccgcc aaataaccat
     1381 gcgaccacat caaactggaa aagaccgata gctccccagt tcagggcacc gattattaca
     1441 agaacaagtg atattctgtc aagcattatt ttcaactcct ttaaaggttt cgccattagt
     1501 atggtctatt aacgccaaga tatacggtct tgatatggta atttattgat gttataattt
     1561 tttaaataaa ataatggaaa aaagcacacg gttctgcctt tatttacagt tttcccaaga
     1621 atacaaatat tattatttta ccggaggaaa ttatgcctgc tgacaaagtt ttaagattta
     1681 acagggacgg caagtttaca atcctcaccg ttgctgacat gcatcttaaa gacaacgggg
     1741 aaaagggaag tgcggatact cttaaactgc ttgaagcgtc gcttattaaa ttcgaccccg
     1801 accttacggt gcttttgggc gacaatacaa gcggctggaa aggcagcagc tttgaaggcg
     1861 ttgaaagggt aatttcgagc ataaccgaga ttttcgacag gcacaaaaag ctgttcgccg
     1921 ttgttttcgg caatcacgac catgaaaccg gtgtttccaa caaagagcag gcagagctat
     1981 ttcaaaagag cgaatactgc ctgttggcag aaggcgattg cgaggtcggc tgcggaaact
     2041 acaatctccc catccttcgc tccgattccg acaaaatggc atttaacctc tggatgatag
     2101 actcaaggga ttactacgaa gacgaaaacg gcaaccgctc ctatgcattt gtttccgacg
     2161 agcaaatcgc ctggtacgaa aagaaaagcg aggaattaaa aaagaaaaac ggcggaatgc
     2221 cggtatactc ccttgtgttc cagcacataa ccgttcccga gcagtacaga cttctcaagg
     2281 cggttcatcc cctgaccccc tttgccgtaa aaggtcacgg agcatggagc gacaggcgct
     2341 ttgtcatcgc ccgccccaaa tacacaaagg gcgctcttgg agaaggtccc tgcccgcccg
     2401 atataaacaa cggtcagttt atgagctggc ttaaaacggg ggatgtaatc ggcgccgtat
     2461 tcggtcacga ccatataaac gactttgtgg gcgatgtgga cggcattaag ctgattcaga
     2521 caaggggagc gggctttacc gcatacggcg acggaatcga ccgcggcgta agacttataa
     2581 ccctttacga aaacgaaccc aaaaagctaa agacggatac atattactac acagaaatcg
     2641 tgggcagcat acccaccaca ctcccgctgc acgaaatgtt gccgctaaga attaaaaaga
     2701 aacttatctg cgcaggctcc gccgcattgc ttacggcctc actgctcgcg gcggcgaaag
     2761 cagtcaatgc gattaaaaaa tgtaagcata gggtgtgata ggcatgaatg tttacgattt
     2821 tgacaacaca atctacgacg gcgaaagctc gcttgcgctg ttttggtttt atattaaaaa
     2881 aatgccccac ttggtcaaat actatcccaa ggttttaaag gcaatgtata actacaaaaa
     2941 gggcaatatc acaattgagg acgcccttca aaaatacata ccaatgatag agccttatgt
     3001 tttgaaggtt atcgacacct gcgataggga cgcaaaagag ttctgggact cgcacatgca
     3061 caaaatcaaa cccttttaca aagagattca gcaggatgat gatgtgataa tcacagcctc
     3121 tcccgaaatc tttatgaggg aaatctgcaa cagactcgga gtcaaagagg ttatcggcac
     3181 aaccttcaat cacgaaaccg gcaaaatcga aaggttctgc tacagaaaaa acaaggttaa
     3241 agcattcaaa gaaagattcg gcgacgctcc gattgacaag ttctataccg actctataaa
     3301 cgacgcgccc cttattgaga ttgcaaagga ggcatatctt gtaaagggca ataaaatcca
     3361 aagaataaag taaaaggcgg aattggcatg agaaacaaaa atgtctccta caaaaacatt
     3421 gatattacgg gcggcttttg gaaggacaga caggatttaa acaaaattgt caccatttat
     3481 gccgttcgtg acagattcaa ggacacaggc aggtttgacg cctttaagtg cgactggact
     3541 gagggcaaac cgaaaaagcc acatttcttc tgggactcag atgtggcaaa gtggatggag
     3601 agcgccgcgt acatactctc aaaggagaga aacgaggagc ttgaaaaaga ggttgacgcc
     3661 cttgttgacc ttatcgaaaa gaatcaggac gaaaacggct atttcaatat ctactttacc
     3721 gttgtggagc ctgatgaaag gttcaccata agagacgccc acgagcttta ctgcgctggt
     3781 catcttattg aggctgccgt cgcatactac gaggcaacgg gcaaggataa gttcctaaag
     3841 ctcatgaaaa agtatgccga ctatatcgaa aaagttttca aaatcgagga ttccgccgag
     3901 tttgcaacac ccggacatga ggagatagag ctggctcttg tcaggctcta ccgctgcacg
     3961 ggtgaggaga aatacttaaa tctctccaag cactttatcg actcccgagg agcaaggggc
     4021 gataaagaag agcttatgat ttacaatcaa agccatatgc ccgtaagaga gcagcgcact
     4081 gccgagggtc actccgtcag agcctgctat ctttacagcg gcatggctga tatcgccgcg
     4141 gagtttgacg acgaggaatt gaaggtggcg cttaacagcc tttttgacaa tatcgtaaat
     4201 agaagaatgt atgtaacggg cggcatcggc tccagtcacc atggcgatgc gttcacaatc
     4261 gactatgacc ttcccaaccc caacgcctat gccgaaacct gcgccgccat atcccttgcc
     4321 atgttcgctg gcaggatgaa tgaaatccgg cccaacggca agtacggcga cattgtcgaa
     4381 aaggtcatat acaacggatt tctgtcgggt ctctcccttg acggaaagag cttcttctat
     4441 gaaaaccccc ttgagattgt aagagccgac agaaccagaa atgtatcatg gcccgccaaa
     4501 gagcgtttcc cgctgacaaa gcgcaaggag gttttcgact gctcctgctg cccgcccaat
     4561 gtcacccgct ttatagcctc catcggcggc tatgtataca ctgtgggcag cgacgaaaac
     4621 accctctatg taaatcagta catggaaagc gtatcgaatt taaagctcgg cgatgccgat
     4681 gtggaaataa cccaaaaaac caactaccca tgggacggca aggtaaatat caccgcaaag
     4741 ggaatgaagg gcaaaaccct ggctctcaga attcccgcat ggagcgaaga attcaccgta
     4801 gaaataaacg gcaagactgc ggatataaaa cccactgacg gctttgctcg tatagaggtt
     4861 gacgctgatg aaatctccgc ggctttgcgg cttgatgtca gccccttctt tgtggagtgc
     4921 aaccccaagg taaacgccaa cgccggcagg gtagctttgc aggcgggtcc cgtggtatac
     4981 tgccttgaag aaatcgacaa cggcccgaat ctcagagata tatccatcag ccgtgagccc
     5041 gatttcaagg taaaatggtt tgacgaactg ggcgttcaaa ttattgaggc taaagccttc
     5101 agaacaaagc cctttgatga gctttacaga cgcgctcaat ttgaaaaaga ggaatgcaaa
     5161 gcggtattca ttccctactt tgctttcgcc aacagagaag agtgcgacat gcttgtgtgg
     5221 actaaagtag agtaaaaaaa tgccccgact ttgagtcggg gctcttttat gtttaattac
     5281 agaattctca gcttctttaa atactccgcc gcgatgtacg catgaccgaa atgggtcggg
     5341 tgaacgccgt cctctgcaag ctccttggga gtgtagtcac gctttacagc cttgttaaat
     5401 attgactgca tgggcagata aatgcagtta tgctttgcag ccagcttttc gcaaacctcg
     5461 attttcgggt caaggtcggg atgccaggtt tccctgtcgg gcagggagtg aaggacaaat
     5521 ggagagataa ccataatctt tgtgtcgggc atgtcggtcc tgattttagt aagcagcaaa
     5581 tcatactcct gctcaaatct ctcaagcggg caggggtcgt tcctgtcaaa tgccctccat
     5641 acatcgttga tgccgatgag tatggagata aaatctggct ttattgcgag gaaatcctca
     5701 tcgtatcttg cgagcaaatc tcttactctg ttgcccgaaa tgcccttatt aataaaggtt
     5761 atgtttctct tgggaaacat ggtgcggtaa atgtttgcca caatctgcgg atagccgttt
     5821 cccagacttg taatgtcgtt tctgtctctg ccgcagtctg ttatgctgtc gccctgaaaa
     5881 agtactactg ccgattcttt aaaatattgt ttcatctaac cattcctgac ttattagatt
     5941 ttgtttacga ttatattgcc gtttgttaca actgccttaa tgtttacatc gtcgtcaaag
     6001 attacaaggt ccgcgtcgta gtttgccttg ataagaccct ttctgtgacc gaagcccacg
     6061 acccttgcgg gagtggtgga tgccatcttt accgagtcaa caaggctgat gtcgcatttc
     6121 ttatacatat tccttacaag gtcagcgcat gtagcgacgc tgccggcaag gcttgagcgg
     6181 tccgccagct tcataactcc gtcctcgtac acaaccgcca aatcattctc ctgagtatag
     6241 atttctccct ctttcatatc cgaggcggag aactcaagac cgtcggtaat ggcgtaagcc
     6301 ctgtcgggac cctttgcctt gtagatgagc ttaatgagcc ctacgggcaa gtgtcttaag
     6361 tcggctatga actgggtggt gtagccgtca agggagagac ccgcctcaac aacgcccgcg
     6421 actctgaaaa ggtttacctt gattaccgac gagcaggcgg agtaaatatg ggtaatatcc
     6481 gaatagccgt agtcaagagc cttgtcgaca atttcatagg ttgcgttgga atgtgcaacc
     6541 gacgccacaa tgcccctctt ggagatttct ttgcccagct ccatgcctcc gggtatttcg
     6601 ggagccgcgc caaccattct gatgcccttc cacaaatcaa gcagctcggt ggggctgaca
     6661 tcaacgggat tgataatatt ttcgggactc tgagcgccct tcatttcaag ggagagataa
     6721 ggcccctcaa gatgcgcgcc caatatgttt gaaaaggtat aatcctcagc cgctttttta
     6781 atagcctcaa cggcggccac gattttgggc atgggagccg caagagtggt cgggagtatg
     6841 gaggtggtgc cccttacagc atgggctttg cacatattct ttatatcctc ggggttgccg
     6901 ctcatggcgg aatatccgcc gccgccgtgg acatgtatgt caacaaagcc gggagcgaga
     6961 taaagaccct ttacatccac cgtttcagcg tctttaatgt caaagcggtc gactatctca
     7021 gctatcttgc cgtctttgac aatcagattg ccttttctta ttctgtcata taaaacaatg
     7081 ttcgcgttct tgtaaacagt tgtgctcata aagacgcctc cttctgcgga aaattttcca
     7141 aaacagaaac cgcagagcaa actttaagaa gaatataaac cctaatgtaa attttgtcaa
     7201 tgagcccgtc tgccttaaac cgtttaagat tttaaatcat aaaaagccca taaactatgg
     7261 aaatttggta tatcctatga tataatcaag tccaaatgat taaaataatt ccaaaatagt
     7321 attatggaaa atttacttgg gggatgctgg gatgtcagaa aaaacagttc gaatcggagc
     7381 cgtgggcatt agcggcagag gctcatcaat gctcaggctc atgatgagat ttaaaggcgt
     7441 tgtctgtccc gccgtttgcg atgtgtatga ggacagaatg aacgacggca ttgagatagt
     7501 taaaaactcg ccccattatt cgggctatga ggtaaaaggc tatcttgact ataaagagat
     7561 gttcgaaaag gagcagcttg acgcggtact tatctgcacc acatggatta cccacgccag
     7621 aatcgcagtt gacgcgatgc gcgctggatt ccatgtcggc cttgaggtcg gcggcgccgc
     7681 ctccgaggag gagtgctggc agatggtcag agcctccgag gcaacgggca agttctgcat
     7741 gctgcttgaa aattgctgct acggacaggc tgagatggca gttttcaata tggtcaagct
     7801 cggtcttttc ggcgaaatcg tataccttac aggcggctat cagcatgatt tgagagacga
     7861 aatcggctac ggcagagaaa tccgccacgg caggctcttt aacttcatgc acagaaacgg
     7921 cgagctttat cccactcatc agcttggccc catcgcaaag ctcataggaa tcaacagagg
     7981 caacagattc ttaagccttg tttccatgtc cacaaaggca aggggactta acgagttcct
     8041 taaaagagaa aagggcgagg gatacgacat agcaaactac cccttcaacc agggcgatgt
     8101 cacaaccaca ataattaaat gcgcaggcgg cgagaatatt actctcattc acgactgcac
     8161 cctgcccaga ccctattcca gaaacgcgag agtccaaggc acaaagggca tctggatgga
     8221 agacggcaac cagatttaca tcgagggcag aagccccttc cacaagtggg aggactttga
     8281 cccttatatg cacgagtttg cccatcctct ttggaggatt cacgcgaaga acgccgcgaa
     8341 atacggtcac ggcggcatgg attatctaat aatggctgcg ttcattgaga gcgcgatgca
     8401 caatctcaaa cctcccattg atgtttacga tacggcatca tggatggcta tcacatatct
     8461 ctccgagcag tcggttgcca tgggcggcat gcccgttccc gttccggatt tcacaaacgg
     8521 cagatggatt gacagagagc cctaccgcag aggcatattc tgccttgaag aagtctgcga
     8581 cgaattcttc accgacgagg atatgaacat tgacgaagaa atattaaaag acagataaag
     8641 catgggggta aaaatgaaat actatatcgg cattgacgga ggcggaacaa aaacacagta
     8701 cgcgcttttt gacgagaata agaaccttat ctcaagcgtt ttcacaggcg gcagcaacca
     8761 cgaaaatctg gagggctcat tctccgaggc tgccgacatc atccacgagg gaatcacaaa
     8821 ccttcttaaa gagaacggtc ttgagtacaa ggatattgag cataccctta tgggtcttgc
     8881 gggcatcgac cacccttacc agcacgacgc tatggttgac gagctggagg agaggggcta
     8941 ttcccgcttt tcaatctaca atgacggctt tatagtaacc aaggcaggca ccgacaaaaa
     9001 ggcggccatc ggctacaact gcggcacggg cacctgctgc aactccatag acagcagagg
     9061 caacatgctg caaatcggcg gcttcggcga cctttcaggc gacgccggca acggcagctg
     9121 gattgcaatc agagccttca gacttgtcta tgacgaaatc tgccttgccc gccacaagtc
     9181 ccttataaca aagctcttta ccgagaaatt cggcgtaaag cccgaaaggg acgagttcct
     9241 tgcaaccatc gctcttttga tggggcttaa gcaagaggaa tatacaagag gacttatcga
     9301 tatattcttt gacgccgcta acgccggcga cgaggtagtt ctcgagtcca ttgaagaaat
     9361 ggctgacagg ggagcggatt tcatcgcggc ccacaccaag ctccagcagt ttgacgatga
     9421 ggttgtggat gttgtccttt ctggctccat acatacaaag ctgcccgccg acatctatat
     9481 agagcgcctt atggaaaagg caagggcaaa gagcttaaag aaactcaact tcataaagct
     9541 tacgggtccg cctgtaatgg gatgcattaa ctggatgctt gaggataaat agcccccgat
     9601 agaaaggaag gatagctgtg aaagaaatag tcgtagccgt aatcggctca ggcagtacat
     9661 attgtcccga gcttgttgac ggttttataa aggctaagga caggctcggc ttaaagagcc
     9721 tcagactcat ggacattgac gagcgcaagc tcaatatcgt cggagacctt tgcataagga
     9781 tgctccgaaa cgcaggtatc gactgcgagg ttataaagac cgacagtctt gacaccgcct
     9841 taaagggcgc ggactttgtc gtaactcaga ttagagtagg caagctcccc gcgcgccatc
     9901 ttgacgaaac catcccgctc aaatacgacc ttatcggtca ggagactacg ggcatcggcg
     9961 gtttcttcaa ggctctcaga accatccccg taatgaagga gattgccgac aaggtaaaag
    10021 agctctgccc cgacgcatgg ctcattaact ttaccaatcc ttcgggtatt atctctgagt
    10081 ttttacagaa ccacaccgac attaagagca tgggtctttg caatgtaccc atcaacatga
    10141 ttgacgatgt caaaaaagct gtgggtcagg acgcggatgt aacatatctc ggacttaacc
    10201 atttaagctg gattacctcc gtcaaaaagg acggcaagga atacatagac aggctctttg
    10261 acgagggctt tgcccccgag gttatggcaa atatccacga cgacggtttt gacatttcct
    10321 gcctcaaggc agcaaggggc attccttctt cctatctcca gtattactac aaccgcgccg
    10381 caaagctcga gtatcttaaa gactccgagc agagcagagc ggaggtttgc atggagattg
    10441 aggaagagct tctgaaaatg tacgccgacc ccgaccttaa agaaaagccc gctctccttg
    10501 atttaagggg cggacacaag tattcccttg cggcggtttc tcttatcgac tcaatcgcta
    10561 acgacacaaa cgacattcat gtggtcaata taaagaacaa cggcaccctc cactttatgg
    10621 aggacgacga tgtggttgaa atcgcggccg ctatcggcaa ggacggcgca aagcccgtaa
    10681 agctcgaaag actcgagaac gagcacatca tcggtcttat gagaatcgtc aaggcatacg
    10741 agcgtctggc cgtaaaggca ggcatggacg gcgatgacga cgcggctctc aacgctctta
    10801 tgctccatcc tcttgtcggc gactttgaaa aggcaaaggc ctgctatgag gaaatgaaag
    10861 aggctcacgc cgcttatctt ccccagttta aaaagtaaac ttaaaacgct gtaaagattg
    10921 ttaatatggc agtctttacg gcgttatatt gctaaaacag ataaaactat aaaggagaat
    10981 gaaggtgcag ggttattcat taaaggtaaa gaaaacaaga cagaaaataa ggaattttta
    11041 cactgcggcc cagggcttgc cctctgatgc aatcaccgct gccgcgtttg acggcaaggg
    11101 tgttctttgg gtaggcaccg acaagggtct tgcaaagttt gacggcaaga agtttgcaaa
    11161 gttcgatgtc aaagcaggaa acggcaatat ctcaatgctt catgtcgata aagaaggcgc
    11221 cctttatgta ggcacagaaa acaagctcgt aaaagtttag tattccataa tcgtcagcac
    11281 catagagctt gtaagcaata t
//
LOCUS       DAONVQ010000061        10655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4789_length_10655_cov_4.028962, whole genome shotgun sequence.
ACCESSION   DAONVQ010000061 DAONVQ010000000
VERSION     DAONVQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4789_length_10655_cov_4.028962"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1066)
                     /gene="argC"
                     /locus_tag="PLD98_05205"
     CDS             complement(131..1066)
                     /gene="argC"
                     /locus_tag="PLD98_05205"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HQA05681.1"
                     /translation="MFRIFIDGAEGTTGLRIYDRLSARKDIELLTLEGPLRKDPEARR
                     KMLNTADISFLCLPDEAAIEAVSLLENENAKIIDASTAHRTKEGWSYGFPELSYELKE
                     RIINGRFTAVPGCHASGFEALVFPLVKRGIISDFLPLSCFSVTGYSGGGKKMIAEYES
                     ESRDKSLDSPRQYALGQNHKHLKEMQYITGLKYKPVFSPVVADFYSGICLTVPLHASQ
                     LTEPMRVNDLREVYAQHYAGSKVISVSFDDDIAHLPANALSGKDSMKIGVYGNDERII
                     LTAVYDNLGKGASGAAVQCMNLMLGLPETAGLELD"
     gene            complement(1245..4145)
                     /locus_tag="PLD98_05210"
     CDS             complement(1245..4145)
                     /locus_tag="PLD98_05210"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQA05682.1"
                     /translation="MKSFKALFDKAKSLDKNKKIAIAVLCVGLVIFAASIIGLLISTE
                     KISLRKPAAPAGDLSDTAPVVSLTVSPEMKNLKAVWFSPFEDLTEKDLSREVIAEHLE
                     AVKSLGFNAVITPLSIEGEKKIINIGETAFDAAGIVADEAKSRGLFLIGSIDSAYYFG
                     KNESSDFDFEAVRSIFAGVPSADALLLKDTDKLTAAFGSDEDNRNALTPALKGFSERF
                     KSSFAGKFCGFSVSSDTEAQDAKHWAESGLADFILVNALNSTANKDMPFTDIADLWNS
                     FNSPSFIYFSHRLDLLREGKEGFDSSSEIIKQAEIVKERENLSGSAFYSYREIREDLL
                     SCASSVASFFSGTGNSEIAAKELEITSPATKKVTTDKSKISFTGVSDPSSLVFLNGKP
                     IERTADGLFSLDLTLKPGVNTFVFTHKGKELKYTVNYEIDLLQEVSPSDKTTAPVGTK
                     IEIAAYAKKGANVYAVVGNAKVQLKATESDLFGKGEGIEDFATFMGTYTLPKINSAKQ
                     SLGKIVVYAEQGGFVESLQGGEIIVTDESNGFSEFILSDSDLNSASLAYPYRNLKNPF
                     TDHSLGRDKLCVVTAEYAEATALSEPSDKSDPKLTPLLKGSIDYVTGRAIYNGKEHYI
                     LKSGRKIYAKDAKLVANAYKMPLNNLRVIRTTNGKTTDIYLKTDWLVPINAELKPQSY
                     YVGFEDRPFNVSTFTASFIDFIFYHTNGVAGLPEFSDNSVLKSAQWLTGKSDKTLKVL
                     RLNLRETGKFYGYKLSVTDEGYIKISFKRKPETLSGMTIMLDPGHGGKKDPGTFTVYP
                     NLYEKEINMKLALIMKRELEAKGAKVIFTRTQDVYLAREDRALFAKQKNPDLYVSIHC
                     DGSTSSSLSGTHSFYYRAFSKPLAESIHKELVSAYRNTIYAPDTKAWQSADRGVKFKP
                     FNVTRIEECPAVLIEFGFLTNASDCNVLINPQYQEVLAKAAVKGIENYVLKQ"
     gene            complement(4150..5043)
                     /locus_tag="PLD98_05215"
     CDS             complement(4150..5043)
                     /locus_tag="PLD98_05215"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQA05683.1"
                     /translation="MYYVVLDLEWNSSYSKKSKSFINEIIEIGAVMVNAGFEVIDTFS
                     TIIKAQLGKRLTSRVKALTNISNEDIRGGVPFSKAFEDFEKWLGGKDTVIMSWGDSDV
                     RVLSDNFLFFNNDERVPFLKKYLDMQKYFQHCYNLPSSKQVGLITAAELCGIDTSDVS
                     SHRALEDSLLALRCLKKIFDEEKMAAFTQECNEDFYKKLRFKVHNITNLRDPHIDKSL
                     MRCDCQNCNKPMQRLSEWKCVNQAFRALFYCRECSNMGLFTIKFRRHFDRVDTHTTFI
                     KLEPAEEESVLTAAQNSSVTG"
     gene            5261..6904
                     /locus_tag="PLD98_05220"
     CDS             5261..6904
                     /locus_tag="PLD98_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HQA05684.1"
                     /translation="MTESKDFVKPNHGSTPQPDHIMLSICGDAKTSMAVTWRTSIDVP
                     SGYVEVYPENGGEKVRVEAITKEFKSDIDISNMHWAIIKNLKPGTRYRYTCGDDTHRS
                     EEFSFETEPENLNKFKFLIITDHQNGTPHHDPQYEPIRKALHDAFSKHPDIRFILTVG
                     DNTNDGQNELQWNGMFRGMKGFIESYPYMMCTGNHDNRGFKQYLPEPVGKFYLMHADF
                     FDAQFEYSYPKNGPEEFETENYSFDYGNVHFAVFGINRQDLVGEWLYEDLQKSDKTWK
                     IGAYHFPIYPIMPEGCNDDSYPWLRKGIEEGRLDVLLAGHEHGLARTYPIKDDQMYDR
                     PSEGTIHYIAGNAGRNPFATNARKIWHPYFYPLEESLYCYIIAEVDDKVLTLTAYLED
                     GRIADVFVIDKNKDLMLPYSPAPIFKRPKMAFKGHIPDLAVRDLYVEEKDGKLYCAFG
                     ALMHSIGGEVIRTKDSVTISVYRQKATFTVGSDIAVCADGDRKLSGVPYMHEKGQIYV
                     PVEDAAEIFGMAWQHAVRNRIVNFDYPSERRPMQTHPKE"
     gene            complement(7050..>10655)
                     /gene="carB"
                     /locus_tag="PLD98_05225"
     CDS             complement(7050..>10655)
                     /gene="carB"
                     /locus_tag="PLD98_05225"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014120347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQA05685.1"
                     /translation="ATTVEEVKNIAKEIGYPVIVRPAFTLGGSGGGVAYNEEELDVIA
                     KNGLLLSPITQVLIEKYIAGWKEIEFEVMRDKVGNTIAVCSMENVDPVGVHTGDSIVV
                     APAVTLANKEYQMLRTAALDIITALGIEGGCNVQFALDPNSFEYAVIEVNPRVSRSSA
                     LASKATGYPIAKVTTKIALGYTLDEINNDITGKTCACFEPAIDYMVVKMPKWPFDKFV
                     DAGRNLGTQMKATGEVMAIAPSFEMAMMKAVRGAEIGLDTLNNPALKDVDIRKQLHSV
                     DDLRIFAVFEALKQGISIDEIYDITKIDRWFIAKLKKLADFEKEIEGKKLTEELYIKG
                     KKLGYPDKALERISGHKLPCHRDAVYKMVDTCAAEFDAETPYFYSTYDDVCESRQHKR
                     SGKERIIVLGSGPIRIGQGIEFDYSSVHCVRTLKNIGYEVIIINNNPETVSTDFDTSD
                     RLYFEPLTPEDVMNVIKVENPIGVVVAFGGQTAIKLTKFLAESNIPILGTSAESIDIA
                     EDRERFDKLLESFNITRPAGLSAMTKEEALKAANTLGYPVLVRPSYVIGGQNMTIAYT
                     DEDVSHYMDLILSQKITNPVLIDKYMMGTELEVDVISDGEDVLIPGIMQHIERAGVHS
                     GDSIAVYPPFSINDIMLKNVIECSEQLALAMKTKGLVNIQYLIYQNKLYVIEVNPRAS
                     RTIPYISKVTGVPMVELATKIMLGAKLKDLGYGTGLYKTPPYYSVKVPVFSFEKLLDA
                     DSSLGPEMKSTGEVLGIGKTLEEALFKGLVSAGFKMPAPSYEKQPGIFITVSHQDKLE
                     IFKLAKTLDDLNFKFYATRGTAKAIEQLGIDVEVVDKLADDNSVMDLLEEGKIDYIVY
                     TGAKRRADAEDFRKLHRRAVQLSVACLTSLDTANALADIIASRFNLNNTELVDINHMR
                     TERQRLRFAKMQGTGNDAIYIDNSSGVITCPESLAINFTNRHYGIGGDSLILIEPSKV
                     ADAKMTVYNKDGSVGEIGGNSIRCVGKYIYDKNPYNLDLKGRETLTIETEKGIRELKL
                     YIYDNQVRSVCVDMGRANFNPKEIPVLIDKDKVISEPYTIGGKEYKITCVSIGNPHCV
                     VFVDKVADVDVPHVGPLFEHAPIFPERVNTEFVRVINRNALKMRVWERGNGETWACGS
                     GACAAVVAAVENGYCNKGEDITVKLKGGDLIVNYTDERILLTGNASLVYEGEIEF"
BASE COUNT         2637 a         2691 c         2510 g         2817 t
ORIGIN      
        1 caattaaagc caaatccttc ttgctcttgt tcttgcggat tccgcagtgg atacccgcag
       61 ccttaaagcc tttagcggcg caaactccgc cttctataaa ctgcaaaaca atcaaccttt
      121 caataaaaat ttagtctaat tcgagtcccg ccgtttcggg aagacccagc atcaaattca
      181 tgcactgaac cgccgcgccc gacgcgcctt ttcccaaatt gtcatacacg gcggttaaaa
      241 taattctttc gtcatttccg taaacgccta ttttcatgct gtctttgcct gaaagggcgt
      301 tcgcgggcag atgggcaata tcgtcgtcga aagaaaccga tattaccttt gagccggcgt
      361 agtgctgagc gtaaacctct cttaaatcgt ttacgcgcat tggctcagta agctgagagg
      421 cgtgcagcgg aacagtcaga caaatgccgc tgtaaaagtc cgccaccact ggggagaata
      481 cgggtttgta tttaagcccc gtaatgtact gcatctcttt tagatgcttg tgattctgcc
      541 ccagagcgta ctgcctcggg ctgtcaagcg acttatctct gctttcgctc tcgtactcgg
      601 ctatcatctt ctttccgccg ccgctgtagc ccgtaactga aaagcaggag agaggcagaa
      661 aatccgagat aatacccctt ttcacaagtg ggaaaacgag agcctcaaag ccgcttgcgt
      721 ggcagccggg gacagccgta aatctgccgt ttatgattct ctctttaagc tcataggaaa
      781 gctccggaaa gccgtagctc cagccctctt ttgttctgtg agccgtggag gcatctataa
      841 tcttagcgtt ttcgttttcc aaaagcgata ccgcctcgat tgctgcctca tcgggcagac
      901 acaaaaaaga aatatcggca gtgttcagca tttttcttcg cgcctcgggg tcctttctca
      961 aagggccttc aagggttaaa agctcaatat ccttcctcgc ggacagacgg tcgtaaattc
     1021 tcaggcccgt tgtgccctcc gcgccgtcaa taaaaatcct gaacaagtca tcagtcctcc
     1081 aaataagttg ccaaattttt ctcctattgt tgtgttaatt atactattta atatgtataa
     1141 agtcaataag aatttaatat ttcattgata tttgtgaata tttatgcaac tatgcccata
     1201 ataaggcccc gaagatgtaa taacatacaa cttcggggct tgggttactg ctttaaaaca
     1261 taattttcaa tgccctttac cgccgctttt gccagaacct cctgatactg aggattaata
     1321 agcacattgc agtcggaggc atttgtgagg aagccgaact caattagcac cgcggggcac
     1381 tcctcaattc ttgtcacatt gaagggcttg aatttgaccc ctctgtcagc gctctgccat
     1441 gcctttgtgt cgggagcgta tatggtgttc ctgtaagcgc ttacaagctc cttgtgaatc
     1501 gactccgcca gtggttttga gaaggctctg taatagaagc tgtgagtacc cgaaaggctt
     1561 gacgaggtgg agccgtcgca gtggatgctt acataaaggt cgggattctt ctgctttgca
     1621 aacagagctc tatcctccct tgcaaggtac acatcctgtg tgcgggtgaa gatgactttc
     1681 gcgcctttag cctcaagctc ccttttcata ataagggcga gcttcatgtt tatttccttc
     1741 tcatagagat tcggataaac cgtaaaggta ccggggtcct ttttgccgcc gtggccgggg
     1801 tcaagcataa ttgtcattcc cgaaagggtc tcgggctttc tcttaaacga gatttttata
     1861 tagccctcat cggtgacgct gagcttgtag ccgtagaatt tgcccgtttc tctaaggttg
     1921 agtctaagca cctttaaagt cttatccgac ttgcccgtaa gccactgggc gcttttcaga
     1981 acgctgttgt cgctgaattc gggcagaccc gcaacgccgt tcgtatgata gaagataaag
     2041 tcaataaacg aagccgtaaa ggtgcttaca ttaaagggtc tgtcttcaaa gcccacatag
     2101 tagctctgag gtttcagctc agcgtttatc ggaaccaacc agtcggtttt taagtaaatg
     2161 tcggtggttt tgccgttcgt ggtcctgata acccttaaat tgttaagggg cattttgtac
     2221 gcgttggcga ccagctttgc gtcctttgcg taaatctttc tgccggattt gagaatatag
     2281 tgctccttac cgttgtaaat cgccctgccc gtcacatagt caatgctgcc ctttaaaagg
     2341 ggagtgagct tggggtctga cttgtcgctg ggctcagaga gagccgttgc ctcagcgtat
     2401 tcagccgtta caacgcagag tttatcccgt ccgagagaat ggtcggtaaa gggattttta
     2461 agatttctat acggataagc taagcttgcg gaatttaaat ccgaatcgga gaggataaac
     2521 tccgaaaaac cgttgctttc atcggttact ataatctctc cgccctgaag cgattcgaca
     2581 aagccgccct gttcggcata gacgactatt tttccaaggc tctgctttgc gctgtttatt
     2641 ttgggcagag tgtatgtgcc cataaaggtt gcaaaatcct caataccctc gcccttgccg
     2701 aaaaggtcgc tttcagtagc ctttagctgt accttggcat ttcccactac cgcatagaca
     2761 ttcgcgccct ttttggcgta tgcggcaatt tcaatctttg tgccaacggg ggctgtggtc
     2821 ttatccgagg ggctgacctc ctgcaaaagg tcaatttcgt aatttaccgt gtatttcagc
     2881 tctttgccct tatgggtaaa cacaaaggtg ttcacgccgg gcttaagtgt caggtccaaa
     2941 gagaaaagac cgtcggcagt tctttcaatg ggcttgccgt ttaagaaaac aagacttgaa
     3001 gggtcgctga cgcccgtaaa gctgattttg gatttatcgg tcgtgacctt ttttgtggcg
     3061 ggggatgtga tttcaagctc ctttgccgca atctcagaat tgcccgtgcc gctgaagaat
     3121 gaagcgacag acgaagcgca gcttaaaagg tcctctctga tttccctgta agagtaaaac
     3181 gcgctgcccg aaagattctc ccgctccttt acaatctcag cctgcttaat tatctcgctt
     3241 gatgagtcaa agccctcttt gccctcacgc agcaaatcaa gacggtggct gaaatagata
     3301 aaggagggtg agttgaatga gttccacaaa tccgcaatgt ctgtgaaggg catatccttg
     3361 tttgcggttg aattcagcgc gtttaccaaa atgaaatccg caagacccga ctcagcccag
     3421 tgctttgcgt cctgagcctc tgtatccgag gaaacgctaa agccgcaaaa cttgcccgcg
     3481 aagctgcttt taaacctctc ggagaagcct ttaagagccg gagttaaagc gtttctgtta
     3541 tcctcatccg agccgaacgc ggctgtgagc ttgtctgtat cctttaaaag cagggcatcc
     3601 gcggaaggaa cgcccgcaaa gatacttcta acagcctcaa agtcaaaatc tgagctttca
     3661 ttcttaccga aataataagc agagtcaatt gagcctatca aaaaaagccc ccttgacttt
     3721 gcctcatccg caactatgcc cgccgcgtca aaagcggtct cgccgatatt tataatcttt
     3781 ttctccccct caatgctaag gggagtgatt acagcattaa agccgaggga tttaacagcc
     3841 tcaagatgct cggctattac ctctctcgac aaatccttct ctgttaaatc ctcaaaagga
     3901 gaaaaccata ccgctttaag gtttttcatt tccggggaaa cggtgaggga taccacggga
     3961 gccgtatcgc tcaaatcccc cgccggagcg gctggctttc tcaaagatat tttttctgtt
     4021 gaaatcagca gcccgattat ggaggcggcg aaaataacca gcccaacgca cagcacagct
     4081 atggcgattt ttttgttttt gtccaaggat ttagccttgt caaataaagc cttaaaagat
     4141 ttcattttct catcccgtaa cagaactgtt ttgtgctgcc gtcaatacgg attcttcctc
     4201 cgcaggctcc agcttaataa aggttgtatg ggtgtcaaca cggtcaaaat gccgcctgaa
     4261 cttaattgta aacagcccca tattagagca ttccctgcag taaaacagag ctcggaacgc
     4321 ctgatttacg catttccact cgctgagcct ctgcatcggc ttgttgcagt tctggcagtc
     4381 gcagcgcata aggctcttgt ctatatgggg gtcccttaaa ttcgtgatgt tgtgaacctt
     4441 gaatctcagt ttcttataaa aatcttcgtt gcattcctga gtaaaggctg ccatcttttc
     4501 ttcatcaaat attttcttaa ggcacctcag agccaaaagg ctgtcctcca aagccctgtg
     4561 ggaggaaaca tcggaggtgt ctatgccgca aagctcagcg gcggtgatga gcccaacctg
     4621 ctttgaggag ggcagattgt agcaatgctg aaagtatttc tgcatatcaa gatatttctt
     4681 taaaaacgga actcgctcgt cgttattaaa gaaaaggaaa ttatcggaca aaaccctgac
     4741 atccgaatct ccccaggaca taataaccgt atcttttccg ccgagccact tttcaaagtc
     4801 ctcaaatgcc tttgaaaagg gcacgccgcc tctaatgtcc tcgttcgaga tatttgtcag
     4861 agctttgacc ctgcttgtaa ggcgcttgcc caattgagcc tttattatgg tcgaaaaagt
     4921 gtcaattacc tcaaaaccgg cattgaccat aacggcgccg atttctatta tctcattaat
     4981 aaagctcttt gatttttttg aatacgaact gttccattcc aggtctaaaa ccacataata
     5041 catcagacgc accctcaaaa aacaatcgaa aacattttac cacagcgaag ctaatttaac
     5101 aattacataa atctacaaaa tcactattat tttttacttt taacgcaatt tgtcgctcta
     5161 tgcgcattat aggtgaattt ccttgatttt ataaactgtt tttaatatac ttatagaaaa
     5221 ttgacttcta taccacattc agtatatttg ggaggacata atgacagaga gtaaggattt
     5281 tgtaaagccg aaccacggct cgacccctca gcccgaccat ataatgctca gcatttgcgg
     5341 cgacgccaaa accagcatgg cggttacatg gagaaccagc atagatgttc cgtcgggata
     5401 tgtggaggtt taccccgaaa acggcggaga gaaggtcagg gtagaggcta tcaccaagga
     5461 attcaagtcg gacatcgaca tcagcaatat gcactgggct atcattaaga atttaaagcc
     5521 cggcacaaga tacagatata cctgcggcga cgacacccac agaagcgagg agttcagctt
     5581 tgaaacggag cctgagaacc ttaataaatt caagttcctt ataatcaccg accaccagaa
     5641 cggaactccc catcacgacc cccaatacga accgatcaga aaggctctgc acgacgcctt
     5701 cagcaagcac cctgacatcc gctttattct gaccgtaggc gacaacacaa acgacggcca
     5761 gaacgagctt cagtggaacg gcatgttccg cggaatgaag ggctttatag aaagctaccc
     5821 ctatatgatg tgcacgggca accacgacaa tagaggcttt aagcaatatc tgcctgagcc
     5881 tgtgggcaaa ttctatctta tgcacgcgga cttttttgat gcccagtttg agtattccta
     5941 tccgaaaaac ggacccgaag agtttgaaac cgaaaactac tcctttgact acggcaatgt
     6001 ccacttcgct gttttcggca tcaaccgcca ggaccttgtg ggcgaatggc tgtacgagga
     6061 ccttcagaag tccgacaaga catggaagat aggcgcgtac cacttcccca tctaccccat
     6121 aatgcccgaa ggctgcaacg acgactccta cccgtggctc agaaagggca tagaggaggg
     6181 caggctcgat gttctccttg cgggtcatga gcacggtctt gcgagaacct atcccataaa
     6241 ggacgaccag atgtacgaca gaccctctga gggaaccatc cactacatag ccggcaacgc
     6301 gggaagaaac cccttcgcca ccaacgctcg aaagatatgg cacccctact tctatcccct
     6361 tgaggaaagc ctctactgct atatcatcgc agaggttgac gataaggtgc tgactctcac
     6421 cgcctacctt gaggacggca gaatcgccga tgtctttgta atcgacaaga acaaagactt
     6481 aatgctgccc tacagtcccg cgccaatatt caaaagacct aagatggctt ttaaaggcca
     6541 tattccggac cttgcggttc gtgacctcta tgtggaggaa aaggacggca agctctactg
     6601 cgcatttgga gcccttatgc acagcatcgg cggcgaggta ataagaacca aagactccgt
     6661 taccatcagc gtttacagac agaaggccac ctttacagtg ggcagcgaca tcgccgtctg
     6721 cgccgacggg gacagaaagc tttccggcgt accttatatg cacgaaaaag gacagattta
     6781 tgtccccgtg gaggacgccg cggagatttt cggcatggca tggcagcacg ccgtaaggaa
     6841 cagaatagta aacttcgact atccgtcgga gagaagaccc atgcagaccc acccaaaaga
     6901 atagcccttt aataagatag cggggaaatc agtgtccgac acttgtccct ctataaatta
     6961 gacgattaaa atctattatt gacgaaaaaa caaaaaagag atgctgaatc agcatctctt
     7021 tttctttttt tctctcggct gtatatcttt tagaactcga tttcaccctc gtaaacaagg
     7081 cttgcgttgc ctgtaaggag gattctttca tcggtgtagt taacaatgag gtcgccgccc
     7141 ttgagcttta cggtaatgtc ctcgcccttg ttgcagtagc cgttttcaac tgctgccaca
     7201 acagcggcgc aggcgcccga accgcatgcc caggtctcgc cgttgcccct ctcccagact
     7261 ctcatcttga gggcgttgcg gtttatgacc cttacaaact cggtgttgac cctttcgggg
     7321 aagatgggcg catgctcaaa cagcgggccc acatgcggca catcaacatc cgcgaccttg
     7381 tcgacaaaca ctacgcagtg cgggttgccg atggaaacgc aggtaatctt gtattccttg
     7441 ccgcctatgg tgtatggctc agaaattact ttgtccttat caatgagaac ggggatttcc
     7501 ttcgggttaa agttcgccct tcccatgtca acgcatacgg agcgcacctg attgtcgtag
     7561 atatagagct ttaattccct gattcccttc tcggtctcaa tggtcagggt ctccctgcct
     7621 ttaaggtcca gattgtaggg attcttgtcg tagatgtatt tgcccacgca gcgtatgctg
     7681 ttgccgccga tttcgcccac gctgccgtct ttgttgtaaa ccgtcatctt cgcgtcggca
     7741 actttggagg gttctatcag aatcagcgag tcgccgccga tgccgtaatg gcggttggta
     7801 aagttaatgg caagggattc ggggcaggtg ataacgccgg agctgttgtc aatgtagatg
     7861 gcgtcgttgc ccgtaccctg catcttggca aagcgcagac gctgcctttc ggtcctcatg
     7921 tgatttatgt ccacgagctc agtgttgtta aggttgaacc tgctggcgat gatgtcagcg
     7981 agtgcgtttg ccgtatcaag ggaggtaagg caggcgaccg agagctgtac cgcgcggcgg
     8041 tggagctttc tgaagtcctc tgcgtcagcc cttctcttag ctccagtata gacaatatag
     8101 tcaatcttgc cttcttccag aaggtccata acgctgttgt cgtcggcgag tttatccaca
     8161 acctcaacat caatgccgag ctgctctata gctttcgcgg tgcctcttgt ggcgtagaac
     8221 ttgaagttca ggtcgtcaag ggtcttggct agtttgaaga tttccagctt atcctgatgg
     8281 ctgaccgtaa tgaatatgcc cggctgcttt tcgtatgagg gagcgggcat cttaaagcct
     8341 gcggaaacga gtcccttgaa cagagcttcc tcaagggtct tgcctatgcc cagaacctcg
     8401 cccgttgact tcatttcggg acccagtgag gagtccgcgt caagcagctt ttcaaaggag
     8461 aatacgggca cttttacgga gtagtacggc ggagtcttgt aaagtcccgt gccgtagccc
     8521 aagtctttaa gcttagcgcc gagcataatc ttggtcgcaa gctcaaccat cggaacgccc
     8581 gtaactttgc tgatgtaagg tatggttctc gatgccctgg ggttaacctc tataacatag
     8641 agcttgttct gatatataag atactgtatg tttacaagtc cctttgtctt catggcaagg
     8701 gccaattgct cagagcactc tatgacattc ttgagcataa tgtcattaat gctgaagggc
     8761 ggatatacgg cgatggagtc gcccgagtga acgcccgcgc gctcaatgtg ctgcatgata
     8821 ccggggatga gcacatcctc gccgtcggag attacatcaa cctccagctc ggtgcccatc
     8881 atgtacttgt cgataagcac ggggttggtg attttctggc tgagtataag gtccatgtaa
     8941 tggcttacat cctcgtcggt gtaagctatt gtcatgttct gaccgcctat gacataggag
     9001 gggcgtacaa gcacaggata gccaagggta ttcgccgcct tcaaagcctc ttccttcgtc
     9061 atggcgctga gacccgcggg gcgggtaata ttgaaacttt caagcagctt gtcgaacctc
     9121 tccctgtcct cggctatgtc aatgctctca gctgaggtgc cgagaatcgg aatgttagac
     9181 tcggcaagga atttggtaag cttaatagcc gtctggccgc cgaacgctac cacaacgccg
     9241 atcgggtttt caaccttgat gacattcata acatcctcag gggtcaaggg ctcgaagtag
     9301 agcctgtcgg aggtgtcgaa gtccgtcgaa accgtttcgg ggttattgtt tatgattatt
     9361 acttcatagc ctatattctt taaggttctg acgcagtgaa cggaggagta gtcgaactca
     9421 atgccctgtc cgattctgat gggacccgag ccgagaacta taattctctc cttgccgctc
     9481 ctcttatgct gccttgactc gcaaacatcg tcgtatgtgg agtagaagta gggagtctca
     9541 gcgtcgaact ctgcggcgca ggtatcaacc atcttatata ccgcgtccct gtggcagggg
     9601 agtttatgtc cgctgattct ctccagagct ttgtcgggat agccgagctt cttgcccttt
     9661 atataaagct cctctgtaag cttcttgccc tctatctctt tttcaaagtc ggcaagcttt
     9721 ttgagcttag ctatgaacca tctgtctatt ttcgtaatgt cgtaaatctc gtcaatgctg
     9781 atgccctgct ttaaagcctc gaatacggcg aatatgcgca aatcgtcaac gctgtggagc
     9841 tgttttctga tatccacatc tttaagagcg gggttattta aggtgtcaag accgatttca
     9901 gcgcccctta cagccttcat catagccatt tcaaagctgg gggctatggc cataacctcg
     9961 cccgtagcct tcatctgagt gccgagattt ctgcccgcgt caacaaactt atcaaagggc
    10021 cacttgggca tcttaacgac catatagtcg attgcgggct caaagcatgc gcatgtcttg
    10081 ccagtaatgt cgttgttgat ttcgtcaaga gtatagccga gagcaatctt tgtggtaacc
    10141 tttgcgattg gatagcccgt agctttactt gcgagagccg atgagcggga taccctgggg
    10201 ttaacctcaa taaccgcgta ctcaaagctg ttggggtcaa gggcaaactg aacattgcag
    10261 ccgccctcaa tgccgagagc cgtaattatg tcaagcgcgg cggtcctgag catctgatat
    10321 tccttgttag cgagtgtcac cgcgggagct acgacaatgc tgtcgcccgt atgaacgcca
    10381 acggggtcca cattctccat gctgcagacg gcgatggtgt tacccacttt gtcgcgcatg
    10441 acctcgaact cgatttcctt ccagcccgct atgtacttct cgataagaac ctgagtaatg
    10501 ggggagagga gaagtccgtt tttggctatg acatcaagct cttcctcatt gtaagcgacg
    10561 ccgccgccgg agccgccgag ggtgaacgca ggacggacaa tgacggggta gcctatttcc
    10621 ttggcaatat tcttaacttc ctccactgtg gtcgc
//
LOCUS       DAONVQ010000062        10633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4800_length_10633_cov_4.338722, whole genome shotgun sequence.
ACCESSION   DAONVQ010000062 DAONVQ010000000
VERSION     DAONVQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4800_length_10633_cov_4.338722"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLD98_05230"
     CDS             complement(<1..792)
                     /locus_tag="PLD98_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HQA05686.1"
                     /translation="MTKGLLIYDREGAKRNKVFIDMLIDRFGKRGIELCLVIFEEMCF
                     GIDSEKPFVSLKDADIKDFSFAVCRCINPLLSRHLELLGLKVFNNSEISRICNSKNMT
                     YSVASELGIPVPITYFGKRGTVLEFNDSDFPLIVKSDSGHGGAEVFWANNMHKLTDAV
                     KRIDADYVVQKPVSELGKDLRIYVVGKTIVGAVLRISEKDFRSNFSLGGSAKLVTPQD
                     NELKIAKKLIDRFDFGMVGIDIIYDNGKPLLNEIEDVVGSRMLYAS"
     gene            complement(785..1678)
                     /locus_tag="PLD98_05235"
     CDS             complement(785..1678)
                     /locus_tag="PLD98_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HQA05687.1"
                     /translation="MRKGWLVVNSFTNWDKFKELYSMLCDAARLHSINLKVKPSYTLL
                     CDITRGSFIIEQGEKPDFIIFWDKDIILAKALEQMGFRLFNSAEAIELCDDKALTHLK
                     LSKAGIRQPKTVSAPKAYPSTKYNDLSFVRLAAEELGFPLIIKERSGSFGFQVHLANN
                     LSEAEEIVKSLEWKPLILQEMIKSSRGRDIRINVVDGKVESAMLRYSTNGDFRSNLTL
                     GGKAEKYDLNKEQKKAALDAVKSLGLDFAGVDMLFGESGEPIICEVNSNAHFKTTLQY
                     TGVNIAEKIIEMIVIRIYAHD"
     gene            complement(1731..4232)
                     /locus_tag="PLD98_05240"
     CDS             complement(1731..4232)
                     /locus_tag="PLD98_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HQA05688.1"
                     /translation="MDKGTIHLIGNAHLDPTWLWQWQIGCGEVLQTFRSALDRLNEYP
                     DFVFTCSSACYYKWVEEIAPDMFEEIKQRIKEGRWVPVNGWWVQPDCNMPSGESFARH
                     ALYSQLYYYEKFGVTCRTGYNVDSFGHNWMLPQLLKKGGMDFYVMMRPHPHENPDIPK
                     GAFWWDSPDGSRVLTFRIPTSYAENGQKAIDRTIEVLEEIIEKNNHPAMLFYGVGNHG
                     GGPTRGDIEYLKSISNRDGRARLEFSNPDDYFNEMLQNYLELPVWRDELQHHASGCYS
                     ATSVIKQLNRKAENLLTAAEKWSTVAYKTAGMPAQNKEFGDAWKEVLYNQFHDILCGC
                     SIMEAYEDAKIQLGAAMDKGSKALNHAQLRIARQVDTWIDGVSDPVYCEVRNKGAERR
                     FPRPVIIFNPHSWDIETPVRILECSEYVKDSEGNEVHFQNIRSSRSNDTHLDTLFVAK
                     VPALGYATYWLKYPENNISITVPEFVDSTINTEEDNKTITIENEYVRVEFDKATGTIS
                     ELINLESGYNYANKGNMAVPTVIDDHKTDTWAHNIFKFHDVKGVMSLVSIELVEKGPV
                     RALVRTKHRFNDSYLYQEFSLAEGQKTIRVKCKAIWQEEFTMLKMAFPTAGRNEISTY
                     EIPCGYIKRPCNGEEEPALQWADLTVTDSEGIRRGISIMSDSKYSYDCPGTELRLTCL
                     RNVIFADHYSYRPPALFNFTDEGLQRFEYGIYLHDGEAEESNIVKEAALFNCRPTYVP
                     ESYHKGTAPQVQSFINIDADNVIVTAIKLCEDGSGDTIIRLYEISGKNVKAAIVCETL
                     GASFFSDFKKHEIKTFRISKDGKVSEVNFLEGIVD"
     gene            complement(4256..6037)
                     /locus_tag="PLD98_05245"
     CDS             complement(4256..6037)
                     /locus_tag="PLD98_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05689.1"
                     /translation="MITPIKLETHLNRFCTFYELNNKAIPEALFKTKSEKPDLSDIHY
                     PLPARKDDYTCYAVTDDGALWLGADNGVARYFPKAERKDDIVLHFSAPRYLYDNNVKA
                     IMADGNAVWVLTETGAVRIEMRPLSPREKANILLEETLKYVDRRGMVSQKKLQVPRDL
                     DSIVSYGHSDNDGCFTAGFAIGEILHYAVLKREKGEDDPETQRIRKIATRASEACLLL
                     MNISGRGNGFVARSYLTPDEPVPDDGLFYRKSGGKAVCLETRASKKRGIAGLEIDAST
                     PVPDRLAALYRERGYSDDGIVYKGDTSSDEMTLHFLHLYFAHKFLAPGDPDLGELIKS
                     SIKATMKHIIDHGNELHECDGKPTTWAKWSLEYFNSPFGWSDACLNAGQILMYLLVTM
                     EITGEQGIWREHYDKLVSLGYADLTTKHHERFYQISLMMNLDCAEELMYGDNMLATAT
                     FWGLLMLEKDKELRKKYKKGFESWRGEIAREYNPGYDFPFKLVCPDAELDPERIKTWF
                     YRFHHSRLAAGVSMTKRFDIPVRKRRGGYLEISALCPPDETFIAKYDRNPRQFRDEDS
                     GGVMCVESCYVYTFAYWIGIYYGLISD"
     gene            6386..7297
                     /gene="nadA"
                     /locus_tag="PLD98_05250"
     CDS             6386..7297
                     /gene="nadA"
                     /locus_tag="PLD98_05250"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQA05690.1"
                     /translation="MIRDIQERILALKKEKDALILAHSYQGREIIEVADFTGDSFQLS
                     VMASKAKNPVVIMCGVRFMAETVKILSPEKTVYLANPIAGCPMAEQMDKELISAVKES
                     LPGYTVVAYINTTAELKTVCDVCVTSSSAVKIVKKIDNDKILFLPDCNLGHYVAQQVP
                     EKEIKLLQGGCPVHAAVTKQDVEIAKAAHPDALLLVHPECIPEVVAMADYVGSTSGIM
                     EYAKKSDAKEFIIGTEMSITEHLQYECPDKKFYYLSKKILCPNMKATTLIDVLNCLEG
                     TGGEVIELSEDVIRDARKCIDRMIELG"
     gene            7290..8381
                     /gene="mnmA"
                     /locus_tag="PLD98_05255"
     CDS             7290..8381
                     /gene="mnmA"
                     /locus_tag="PLD98_05255"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQA05691.1"
                     /translation="MADTKNTTKKAVIAMSGGVDSSVAALLMKNKGYECIGVTLKLFN
                     DDDINRCNERTCCSFDDVQDAKQVAYALGIPHYVFNFSDEFKDTVIKRFVDSYLKGAT
                     PNPCIDCNRFIKFQKLIHRTIETGFDVMATGHYARIEYDSGSKRYLLKKAFDKTKEQS
                     YVLYAMTQEQLARTEFPLGELTKDEVRRIAEENGFVNAKKRDSQDICFVPDSDYAAFI
                     EGYTGIKSPKGYFVDENGNILGEHNGIIRYTIGQRKGLGLSLKKPMYVCDKDPVTNTV
                     KLCENSGLFEKELTASDINLIACDSIQGRVRLTAKIRYKHPEQPCFVEQIGEDKLHVV
                     FDEPQRAITKGQALVLYDGDTVVGGGTVD"
     gene            complement(8436..10298)
                     /locus_tag="PLD98_05260"
     CDS             complement(8436..10298)
                     /locus_tag="PLD98_05260"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HQA05692.1"
                     /translation="MAFWLMRQFSIEKIVKRLISSFSIVYFAYVFIKTVDFNKPGYLS
                     GFKYGIFALIVLGVFLLQTIVFSFIKYKNADTFFLFGSVMLLFSYLLYEYSSIYLYII
                     LALIFALAVKYYLYDNDFNPEIPKTFMKGCIIGLSIFFVVFVGGLSVLRHLNYKSNCF
                     DFGIFAQMYHNMKTTGLPITTCERYSILSHFKVHISPIFYLLLPIYFIFPFAETLLIA
                     QSLLIISGLIPLVLLCRHYKLSNTVTFLVAACYTFYPAFMGGAFFDFHENKFLPPIIL
                     FLMYFIEKNNLWGIISCSALTLLVKEDAPLYVAFIAIYMMAEKKKYLRGAVLFAVSVI
                     YFAAAVYYLQNFGDGAMTDRYNNYVTEGSGIVGLVKSSILNPAFVLSQAVTEDKLTFI
                     IQMLGPLAFIPFVNRKFTLYILLGPFLVMNLLAGYWPQASISHQYIYGSGCLLFYIFI
                     VSLSHYKPNTRAAVAVFCAVSSLMLFSGTKAVKVNILFDYLENREKHAIIEECLNLVP
                     ENASVTANGYLVPHLYKREKIYQFAYSQEFLERLYADGEDFVIDDKSLINGSPDQEFI
                     ETKDRYLDTEYTVLLLDTNIGRRCFLKLEESGFETVFYRQDVIVLLKNPYYVPH"
     gene            complement(10358..>10633)
                     /locus_tag="PLD98_05265"
     CDS             complement(10358..>10633)
                     /locus_tag="PLD98_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05693.1"
                     /translation="PRSIIGFDPATLPRDRKTGAIQKNKLYRIADYIVLDLRFTKNRE
                     DCKTLIDIGYEPNIYDSKTIAVLRNPNSTKPRLGTVESLDDLVVPTH"
BASE COUNT         2817 a         2413 c         2485 g         2918 t
ORIGIN      
        1 gcttgcgtac agcattcttg agcccacaac atcctcaatt tcatttagga gaggtttgcc
       61 gttgtcgtaa attatatcaa tgccaaccat gccgaagtca aagcggtcta tgagcttttt
      121 tgcaattttc agctcattat cctgaggtgt aacgagtttt gccgagccgc cgagtgagaa
      181 attgcttctg aaatcctttt ctgaaatcct gagcaccgcg ccgactatgg ttttgcccac
      241 aacatagatt ctcaaatcct tgccgagctc ggatacgggc ttttgcacaa catagtcggc
      301 gtcaatccgc tttacagcat cggtcagctt atgcatattg ttcgcccaaa aaacctcagc
      361 gccgccgtga cccgaatcgg actttacgat aagaggaaag tcgctgtcat taaactctaa
      421 aaccgttcct ctctttccga aataagtgat gggaacgggg atgccgagct cagaagctac
      481 agaatatgtc atgttcttac tattacatat tcttgagatt tcggaattat taaatacttt
      541 aagccccaat agctcaagat ggcgtgacag caggggattt atgcatctgc aaacggcaaa
      601 gctgaaatcc ttaatatccg catcctttaa actaacaaag ggcttttcag agtctatccc
      661 aaagcacatt tcctcaaata taacaaggca aagctctatg cctcttttgc cgaatctgtc
      721 tatcagcatg tctataaaga ctttattccg ctttgcgcct tctctgtcgt agataagcaa
      781 acccttagtc atgagcgtaa atccttataa caatcatttc tataatcttc tccgcgatgt
      841 tcacgccagt gtactgcaga gtggttttaa aatgggcgtt ggagtttacc tcacaaataa
      901 taggctcgcc gctttcgcca aaaagcatat ccacgcccgc aaaatcaagc cccaaagact
      961 taactgcgtc gagcgccgcc tttttctgtt ctttatttaa atcatatttt tcggctttgc
     1021 cgccaagggt aaggttagac ctgaaatccc cgttagtgct gtatctcagc atggcggact
     1081 ctaccttgcc gtcgacaaca tttattctta tatccctgcc tcggcttgac ttaatcattt
     1141 cctgcaatat aagcggcttc cattcaaggc tttttactat ttcttcagcc tcagaaagat
     1201 tatttgccaa atgcacctga aagccgaatg agccgctgcg ctctttaatg ataagcggaa
     1261 agccaagctc ctcagcggct agccttacaa agcttaaatc gttgtatttg gtggaggggt
     1321 aagcctttgg agcggaaacg gttttcggct gccttatgcc cgctttgcta agtttgagat
     1381 gggtcagcgc tttatcgtcg cacagctcaa tagcctcagc cgagttaaag agcctaaaac
     1441 ccatttgctc gagtgccttt gcgaggatta tatccttatc ccaaaagata ataaagtcgg
     1501 gcttttcgcc ttgctctatt ataaagctgc cccttgtaat atcgcataat agggtatagg
     1561 agggcttaac cttaagatta atactgtgca gccttgccgc gtcgcagagc atggagtaca
     1621 gctccttgaa cttgtcccag ttggtaaagc tgtttacaac aagccagcct tttctcattt
     1681 tgcctccatt ttttaaagac tcctgccgct tacggcagga gtcttgctct ttagtctaca
     1741 attccttcaa ggaagtttac ctcagaaacc ttgccgtctt tggagattct gaaggtcttg
     1801 atttcgtgct ttttaaagtc ggagaagaac gaggctccaa gagtttcgca tacaatagcg
     1861 gcctttacat tctttccgga gatttcgtaa agccttatta tagtatctcc cgaaccgtcc
     1921 tcgcagagct taattgctgt gacaattaca ttgtctgcgt ctatgtttat gaagctctga
     1981 acctgaggag cggtgccctt atggtagctt tcgggcacat aggtgggacg gcagttaaag
     2041 agagcggctt cttttactat gttggattcc tcagcctcgc cgtcgtgcag ataaatgccg
     2101 tactcgaatc tctgcagacc ctcgtctgtg aagttgaaca atgcgggcgg acgatatgaa
     2161 taatggtcgg caaagattac atttctaagg caggtgagtc tgagttcagt gccggggcag
     2221 tcatagctgt acttggaatc gctcattatg gaaatgccgc gccttatgcc ttctgaatcg
     2281 gtaacggtaa ggtctgccca ctgcagtgcg ggctcctcct cgccgttgca gggtctctta
     2341 atatatccgc agggaatctc ataggtggaa atctcattcc tgcccgcggt tgggaaggcc
     2401 attttaagca ttgtaaactc ctcttgccaa atagccttgc acttgactct tatagtcttc
     2461 tgaccctctg cgaggctgaa ctcctgatac agatacgaat cgttgaacct gtgctttgtg
     2521 cgtacaagag cgcgaacggg acctttttca acaagctcaa tcgaaacaag gctcataacg
     2581 ccctttacat cgtggaactt gaagatattg tgcgcccaag tgtccgtctt atggtcatca
     2641 ataacggtgg gaacagccat attgcccttg tttgcgtaat tgtagccgct ttcaaggtta
     2701 ataagctctg agatggtacc cgtagcctta tcgaactcaa cgcggacata ctcgttttcg
     2761 atggttatgg tcttgttgtc ctcttcggta ttgattgtgg aatccacaaa ctcgggaacc
     2821 gttatggaga tattattctc gggatacttg agccagtagg ttgcatagcc cagtgcggga
     2881 accttagcga caaacagagt gtcaaggtga gtgtcgttag aacgggatga gcggatgttc
     2941 tggaaatgaa cctcgttgcc ctcagagtcc tttacatact cagagcattc aagaatgcga
     3001 accggggttt cgatatccca agaatgaggg ttgaagatga ttacggggcg ggggaatctg
     3061 cgctctgcgc ccttgtttct gacctcgcag taaacagggt cggaaacgcc gtcaatccaa
     3121 gtatcaacct gtcttgcgat tctaagctgc gcatggttca aagcctttga acctttatcc
     3181 attgcggcgc caagctgaat cttggcgtcc tcgtaagcct ccatgatgga gcagccgcag
     3241 agaatgtcgt ggaattggtt atacagtact tccttccaag cgtcgccgaa ctccttgttc
     3301 tgagcgggca tgcctgctgt tttgtaagct acggtgctcc acttttcagc ggcggtaagg
     3361 agattctcag cctttctgtt aagctgcttt ataacggatg ttgcggaata gcagccgctt
     3421 gcgtggtgct gcagctcatc tctccaaacg ggcagctcaa ggtagttctg gagcatctca
     3481 ttgaaatagt cgtcgggatt ggagaactca agtcttgctc tgccatctct gtttgatatg
     3541 gacttgaggt attcaatatc gccccttgtc ggtccgccgc cgtggttgcc cacgccgtag
     3601 aacagcatgg cggggtgatt gttcttttca ataatctctt caagaacctc tatggtcctg
     3661 tctattgcct tttgtccgtt ttcggcatag cttgtgggga ttctgaaggt taaaaccctt
     3721 gagccgtcgg ggctgtccca ccagaacgcg ccctttggaa tatcggggtt ttcatgcgga
     3781 tgcgggcgca tcattacata aaagtccatg ccgcccttct taagcagctg cgggagcatc
     3841 cagttgtggc cgaatgagtc tacattatag cctgtcctgc aggtaacgcc gaatttctca
     3901 tagtaataaa gctggctgta aagagcgtgc cttgcgaagc tctctcccga gggcatgttg
     3961 cagtcgggct gaacccacca gccgttgact ggtacccagc ggccctcttt aatcctttgc
     4021 ttgatttctt caaacatatc cggggcaatt tcttctaccc atttgtagta gcaggcggag
     4081 gagcaggtaa atacaaaatc cggatattcg ttcagtctgt caagggcaga gcggaaagtc
     4141 tgcagaacct cgccgcagcc tatctgccac tgccaaagcc atgtggggtc aagatgggca
     4201 ttgcctataa gatggattgt acctttatcc ataagtattc tccttatatc tgtatttagt
     4261 ctgagataag tccgtagtaa atgcctatcc agtaagcaaa ggtgtagaca tagcagcttt
     4321 caacgcacat tacgccgcct gagtcctcat ccctgaattg cctcgggttt cggtcgtatt
     4381 ttgcgataaa ggtttcatcg ggaggacaga gagcggaaat ctcaagatat ccgccgcgtc
     4441 ttttcctgac gggaatatcg aacctctttg tcatgctgac gcccgctgca agcctgctgt
     4501 ggtggaagcg gtagaaccat gtcttaatcc tttcggggtc aagctccgcg tcggggcata
     4561 cgagcttaaa ggggaagtca tagccggggt tgtattctct tgcgatttcg cctctccagc
     4621 tctcaaagcc tttcttgtac ttttttctga gctctttatc tttttcgagc atcaaaagtc
     4681 cccagaaagt ggcagttgcc agcatattgt cgccgtacat aagttcctcg gcgcagtcaa
     4741 ggttcatcat aaggctgatc tgatagaaac gctcgtggtg ctttgtggta aggtcagcat
     4801 agcctaagga taccagcttg tcataatgct ctctccaaat gccctgttcg cctgtaatct
     4861 ccatggtgac aagcaggtac ataaggattt gacctgcgtt aaggcaggcg tctgaccagc
     4921 cgaaggggga gttaaagtat tcaaggctcc attttgccca tgtggtgggc ttgccgtcac
     4981 attcgtgcag ctcgttgccg tggtcgatta tatgcttcat agtggcttta atggagctct
     5041 taataagctc gccgagatcg gggtcgccgg gagcaaggaa cttgtgagca aaataaaggt
     5101 gcaggaagtg cagcgtcatc tcgtcgctgc tggtatcgcc cttgtaaaca attccgtcgt
     5161 cggagtagcc cctttctctg taaagggctg cgagcctgtc gggaacggga gtgctggcgt
     5221 caatctccaa gccggcgatg cctcttttct tgcttgccct tgtttcaagg caaaccgcct
     5281 tgccgccgct ctttctgtag aaaagaccgt catccggcac tggctcgtcg ggagtaagat
     5341 atgaccttgc aacaaagccg tttcccctgc ctgagatatt cataagcaac aggcaggctt
     5401 cgctggccct tgtggcaatc tttctgattc tctgagtttc ggggtcatcc tcgccctttt
     5461 ctcttttaag aaccgcatag tgcaggattt caccgatggc aaagcctgct gtaaagcagc
     5521 cgtcattgtc ggagtggccg tagctgacta tggagtcgag gtctctggga acctgtagtt
     5581 tcttttggct taccatgcct cttctgtcaa catatttaag ggtttcttca agcagaatgt
     5641 ttgctttttc tctgggggag agtggacgca tttcgattct aaccgctccc gtttcggtca
     5701 gcacccaaac ggcgttgccg tccgccataa tagcttttac attgttgtca taaaggtagc
     5761 ggggagcgga aaagtgaaga acgatgtcat ccttcctctc tgctttgggg aaataccttg
     5821 caacaccgtt atcggcgccg agccataaag ctccgtcgtc tgtcacggca tagcaggtat
     5881 aatcatcttt tcttgcgggc agggggtaat gaatgtccga aaggtcgggt ttttcagact
     5941 ttgtcttaaa cagagcttcg ggaattgcct tattgttcag ctcataaaaa gtgcaaaacc
     6001 tgttaaggtg ggtttcaagc tttataggtg ttatcatgtc aagctccttt ctcagtttcc
     6061 ttataggccg gcaaaacaca attttccatt gtgaattaat catttagata gtataatacc
     6121 ctgctttaag tgtcaaccag tctaaagcaa ttttggtaaa tactattatt tatgctgata
     6181 ttattgtata ctttgctaaa gcggcgaatt aaaccccatc tgacgccaat aataattttg
     6241 actgttcttt ttatacaaat aaatgctgct tttaaaaaga accttgtgcc ttgggcttat
     6301 tgctttttcg acataattgt aatattataa actcaggatg atattcacta ttatttcgtc
     6361 aaagaacgct aatcggagga gctaaatgat tagagatata caagagagaa ttttagcatt
     6421 aaaaaaggaa aaggatgctc ttattcttgc tcacagctat cagggtaggg agataatcga
     6481 agttgccgat tttacgggcg actccttcca gctcagtgta atggcaagca aggcaaagaa
     6541 tcctgttgtt ataatgtgcg gcgtcagatt tatggcggaa accgtaaaaa tactttctcc
     6601 agaaaagacg gtttatctcg caaatcccat agctggctgt cccatggcgg agcagatgga
     6661 caaggagctt atttcagcgg ttaaagagag ccttcccggc tataccgtgg tcgcgtacat
     6721 caacacaacc gccgagctta aaaccgtttg cgatgtctgc gttacatctt cctcagcggt
     6781 taagattgtt aagaagatag ataacgataa gattttgttc ctgcccgact gcaacttagg
     6841 tcactatgtt gctcagcagg ttcccgaaaa agaaattaag cttttgcagg gcggctgccc
     6901 cgttcacgca gcggtaacaa agcaggatgt ggagatagca aaggcggctc accccgatgc
     6961 ccttctgctg gttcaccccg agtgtatccc cgaggttgtt gcaatggctg attatgttgg
     7021 ttccacttcg ggaataatgg aatatgccaa aaagtccgac gcaaaagaat tcatcatagg
     7081 caccgaaatg agcataaccg agcaccttca gtacgagtgc cccgacaaaa agttctatta
     7141 cctctcaaag aaaatccttt gccccaatat gaaggcaacg actctcatcg atgtgcttaa
     7201 ctgccttgaa ggcacgggcg gagaagtcat tgagctttca gaggatgtta ttcgagatgc
     7261 ccgcaagtgc atagacagga tgattgagct tggctgatac aaaaaacaca accaaaaagg
     7321 cggttatagc aatgagcggc ggagttgaca gctctgtcgc tgctttgctg atgaaaaata
     7381 agggctacga gtgcattggc gtaactttaa agctctttaa cgacgatgac atcaaccgct
     7441 gcaatgaaag aacctgctgc tcctttgacg atgttcagga cgcaaagcag gtggcatacg
     7501 ctttgggtat tccccattat gtttttaact tctccgatga gtttaaggat acagtcataa
     7561 agaggtttgt cgattcatac ttaaagggcg cgaccccgaa cccttgcatt gactgcaaca
     7621 gatttataaa gttccagaag ctgattcaca gaaccataga aaccggcttt gatgtcatgg
     7681 caactgggca ttacgcaaga attgagtacg acagcggctc caaaagatac cttcttaaaa
     7741 aggcatttga caaaacaaag gagcaaagct atgtactcta tgccatgact caggagcagc
     7801 ttgcaagaac cgaattccct ttgggcgagc ttacaaagga tgaggtaagg cgaattgccg
     7861 aagaaaacgg ctttgtaaac gccaaaaagc gtgacagtca ggacatatgc tttgtgccgg
     7921 attccgacta cgccgcattt attgaaggtt atacgggcat taagagccca aagggctact
     7981 ttgtggacga aaacggcaat attctgggcg agcataacgg aataatcaga tacaccatag
     8041 gtcagcgcaa gggtcttgga ttgtcgctaa aaaagcccat gtatgtctgc gataaggacc
     8101 ctgtgacaaa tacggttaag ctctgcgaaa acagcgggct ttttgaaaag gaacttaccg
     8161 cctccgacat aaatcttata gcctgcgatt cgatacaagg cagagtccgc cttacggcaa
     8221 agataagata taagcacccc gagcagccgt gctttgtaga gcagataggc gaagataagc
     8281 ttcatgtggt ctttgatgaa ccccaaaggg ctataacaaa gggtcaggcg cttgtgcttt
     8341 acgacggcga cactgtcgta ggcggcggaa ctgtcgacta aagaagaaac ccccgagatt
     8401 gcttgggggc ttttttatgg gagatttgag agaaatcaat gcggaacata atagggattt
     8461 ttgagaagca ctataacatc ctgacgatag aacaccgttt caaagccgga ttcttcgagt
     8521 tttaaaaagc atcttctgcc tatatttgtg tcaagcagca gaacggtgta ttcggtatca
     8581 aggtatctgt ctttggtttc gatgaattcc tggtcagggc tgccgttgat taaggatttg
     8641 tcgtcaatca caaagtcctc gccgtcggca taaagtcttt ccaggaactc ttgagaatat
     8701 gcaaactgat atatcttttc ccttttataa aggtgcggaa caagatagcc gtttgcggtt
     8761 acacttgcgt tttcgggcac aaggttaagg cattcctcta ttatcgcatg cttttcacgg
     8821 ttctcgagat aatcgaaaag tatgttgact ttaacagcct ttgtgcctga gaaaagcata
     8881 agagaggata cagcgcagaa caccgcgacc gcggctcttg tgttcggttt atagtgggac
     8941 aaggatacta taaaaatata aaagagcagg catcctgagc cgtatatata ctgatgactg
     9001 attgacgcct gaggccaata tcccgcaagc aggttcataa ccaaaaaggg gcccagaaga
     9061 atataaagag taaatttcct gttcacaaag ggtataaagg ccaagggacc cagcatttga
     9121 attatgaaag taagcttatc ctctgttacg gcctgagata gcacaaacgc ggggttaaga
     9181 attgaagact ttacaagacc cacaatgccc gaaccttcag tcacatagtt gttgtacctg
     9241 tcggtcatgg ctccgtcgcc gaagttttgc agataataca ccgcagctgc aaagtagata
     9301 acggaaaccg caaaaagtac tgctcctctt aaatacttct tcttttccgc catcatataa
     9361 attgcgataa acgccacata cagcggcgcg tcctctttga caagcagggt aagcgctgag
     9421 caggatataa ttccccacag attgttcttt tcaatgaaat acataaggaa caatataatc
     9481 ggcgggagga atttgttttc atgaaagtca aagaacgctc cgcccataaa tgctggataa
     9541 aatgtgtagc aggcggccac aaggaatgta acggtatttg aaagcttata atgcctgcaa
     9601 aggagaacaa gcggaataag accgctgata attagcagag actgagcgat aagcagagtt
     9661 tcagcaaatg gaaagataaa gtaaatgggt aatagcaaat agaaaatggg agagatatga
     9721 accttgaaat gagaaagaat tgaatatctt tcgcatgtgg ttatgggcag acccgttgtt
     9781 ttcatattgt gatacatctg agcgaagatg ccgaagtcaa agcagttgct tttatagttt
     9841 aggtgcctta aaaccgacag cccgcccaca aataccacga aaaatatgct aagcccaata
     9901 atacagccct tcataaaggt tttgggtatt tcggggttga aatcgttgtc ataaagataa
     9961 tactttaccg caagagcaaa aatcaaagcg aggataatgt aaagataaat actcgaatac
    10021 tcatacaaaa gatacgagaa caggagcatc acgctgccga acaagaagaa tgtgtcggca
    10081 tttttgtatt taataaatga gaatacaatg gtttgaagca ggaatactcc caaaacaata
    10141 agggcgaata ttccgtactt aaaacccgaa agatagcccg gcttattaaa gtccacagtc
    10201 tttataaaca cataggcaaa ataaactatg ctaaatgagc ttataagcct tttaactatc
    10261 ttttctatcg agaattgtct cattaaccaa aacgccattt tagcttacct ccgaagccga
    10321 gcccttacga cctgaattta agtgtgactg ttcctattca atgagtcggc actacaaggt
    10381 cgtcaaggct ttcgaccgtt ccgagtctgg gttttgtgct gttggggttt ctcagaacgg
    10441 cgattgtttt gctgtcataa atgtttggtt cataaccgat atcaataaga gttttgcagt
    10501 cttctctgtt ctttgtgaat ctcaaatcaa gaactatata atcggcaatt ctgtaaagct
    10561 tatttttctg aattgctccg gttttcctgt ctctcggcag cgtagcgggg tcaaaaccta
    10621 taatgctgcg ggg
//
LOCUS       DAONVQ010000063        10551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4842_length_10551_cov_3.882812, whole genome shotgun sequence.
ACCESSION   DAONVQ010000063 DAONVQ010000000
VERSION     DAONVQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4842_length_10551_cov_3.882812"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLD98_05270"
     CDS             <1..699
                     /locus_tag="PLD98_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HQA05694.1"
                     /translation="IGAVPPHDGQEVTILGIISGVTRKITKDDKTMAFFKLEDMDGTI
                     EVIVFAKIYEQYASLLVEDSVVMVTGRLSVREDEKTKIICDKIVDGETVVRACRQSSA
                     PPPPEYTPIFSSLKTPEISTEPAANGTIKGSEESNTTVQQNKELCMPTNGNGNGNGGC
                     RKGLFLRLSSKTAPEFIKASSLVDIFAGDLPVYYYFADTKVYVNSGKRVLINPTMLSE
                     LKYILGEKNVVMRS"
     gene            715..2112
                     /locus_tag="PLD98_05275"
     CDS             715..2112
                     /locus_tag="PLD98_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA05695.1"
                     /translation="MSAALKFFLVSDLHLYASCFEDDKRLLKPDQKFIAESGAIIDSA
                     FNLMAKNKDINIVLIAGDLTYNGELESHKEMIAKLYRLKNAGKRVFVITATHDFGKEP
                     SVKYKGSDRLAETTPREYLAKLYYPFGRNEAISEDEESTSYCVKLAESYRLLCLNDDS
                     NGKGRNGFSDKTLKWILAQIADARRAGDTIFAMTHHPVLPPSPIYELIAKRDMLTDGD
                     KISDLFADAGLEFIFTGHSHMQNISVKLSKNGNRFYDINTGSLVGYPNPIRVVTLDDE
                     AMRVETINVTDFKWDFDGVEPQEYLKNNFSSLLNEALDAAANDIEKLAQLAVGISVKP
                     ESILKLRVPITLFGRFINKLTVGQLGKMFFISSKIDKSIENLLVKNVIIDIFQNLYCG
                     DEPYYPGTPIYTAVEALFDRILLLTKRSKYYGKIKEILYTIKDGVLYDAPPSDNNAVL
                     PRQNRPLKEAERMII"
     gene            2109..3047
                     /locus_tag="PLD98_05280"
     CDS             2109..3047
                     /locus_tag="PLD98_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HQA05696.1"
                     /translation="MSTKTDSITHKENNAEFKPFKGFFKRLIFAFIIGVAFITPGLSG
                     GVIAAAAGIYEPSVNAIVTIHKNFRKSFFYLLPIGIGAVSGVLIFSRILEQIIQSYET
                     TLIFIFLGLVIGSIPAFIKEANSEGFKKIYAIPTVLTFALVMVIEKILSQPAFHNAAA
                     ELTLPMFIVCGIIMSLGIIIPGISSSFILMAMGIYNGLLSTINSWVDNVFSFIKGGFK
                     GFGAFLASFDWASIFALGLSFAITSIIAIKLVDILFKRYHGVAYYSVFGFLLGSMVAA
                     FPGFRGGIGTAIDIAAMAAAVAIGYFGMKLNGKVKD"
     gene            3047..3700
                     /locus_tag="PLD98_05285"
     CDS             3047..3700
                     /locus_tag="PLD98_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HQA05697.1"
                     /translation="MGRFEHIFFDLDGTLTDPAEGITNSIAYVLDYYKIDYPSKKALE
                     RHIGPPLHKTFITEYGFDENSAELAIQKYREYFSDKGIFENRLFDGIPEMLSGLKAKS
                     KKIYLATSKPQYYAKKILEHFDIAKYFDFVSGCELDGTRSQKTEVIEHICENVGLKDL
                     SSSVMVGDRKYYIEGAHHFGMPCIAVLFGYGSMEEFKEAKADYIVESVEELYNLLIS"
     gene            3866..5155
                     /locus_tag="PLD98_05290"
     CDS             3866..5155
                     /locus_tag="PLD98_05290"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005366074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQA05698.1"
                     /translation="MNLVLANGFVFADGGFVKSNVRISGGKIISLGIQTCEFADAEII
                     DCSNMYIIPGFVDVHVHLREPGFSYKETIASGTAAAAKGGYTALCLMPNLNPVPDTME
                     NIKLQLDIISKDALVKCYPYASITKGRTGRGEITDFHELKKYACAFTDDGTGVNDPET
                     LELVAKTAKAENIIIAAHCEDETLVNGGYIHAGEYAKRNGHKGIVSESEWLPLKRDIE
                     YAEKYGFHYHVCHVSTKESVELIRQAKKRGVRITAETAPHYLVFTDEDLEDDGRFKMN
                     PPIRSAEDRKALIEGIIDGTIDIIATDHAPHSAEEKSKGLKDSLMGIVGLETAFPVMY
                     TKFVKEEIISLEKLIEIMAIKPRKIFRLPDSSINGIAEGQAADIAVLDLNTEYKINPD
                     TFKSKGRSTPFAGMTVNGECVMTLVDGKIVYKKADKR"
     gene            5160..5897
                     /locus_tag="PLD98_05295"
     CDS             5160..5897
                     /locus_tag="PLD98_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HQA05699.1"
                     /translation="MRQLLFEAENIRAIADKVYELKLTGDTTEIVRPGQFVNIRVIGK
                     YLRRPISVCDWDSESLILIFKVFGEGTEILSRIKKGEKMDILSGLGNGFTVIETDKPL
                     LVGGGVGVPPLYGLAKKLIENGLKPNVVLGFASAKDVFYKDEFESLGLKAYVATVDGT
                     MGTKGFVTDAIKENNIECDYFYACGPNPMLKALCSELECSGQLSLEERMGCGFGACMG
                     CSINTAKGIKRVCHDGPVFFKEDLLWK"
     gene            5888..6799
                     /locus_tag="PLD98_05300"
     CDS             5888..6799
                     /locus_tag="PLD98_05300"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009536960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HQA05700.1"
                     /translation="MEVNMKVNLSGVELDNPIIPASGTFGFGEEFKDLYDLNILGSIS
                     FKGTTREPRFGNPTPRIAECPSGLLNSVGLQNPGIDAVINEEIPKLSKYFHKPLIANI
                     SGFSIEDYAFACEKIDKVEQVGIIEVNISCPNVKHGGMSFGTSPESAAEVTKAVKEVT
                     TKPVYIKLSPNVTDIAAIARACEAAGADGFSLINTLLGMRIDIKKRKPVLANVVGGLS
                     GPAVFPVALRMVYQVYKAVSVPIIGMGGISSASDVFEMMMAGASAVQIGSANLVDPFI
                     CKKIIEELPIKMQELGISSLSELKPDF"
     gene            6808..7506
                     /gene="pyrF"
                     /locus_tag="PLD98_05305"
     CDS             6808..7506
                     /gene="pyrF"
                     /locus_tag="PLD98_05305"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQA05701.1"
                     /translation="MKRDVIIACDFNSAEAVYSFLDKFTDVKPFVKIGMELFYAEGPA
                     IVREIKKRGYKIFLDLKLHDIPTTVKKSMAVLSALDVDIVNLHAAGTSEMMKAALEGL
                     TRPDGTRPLLIAVTQLTSTSEERMQKELLINATMEETVAHYALNAKNSGLDGVVCSPL
                     EAGIVAKACGEDFIRVTPGIRFADGDVGDQVRVTTPAKAKELGATFIVVGRPITAADD
                     PVAAYKRCVEEFVN"
     gene            7529..8179
                     /gene="pyrE"
                     /locus_tag="PLD98_05310"
     CDS             7529..8179
                     /gene="pyrE"
                     /locus_tag="PLD98_05310"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQA05702.1"
                     /translation="MANYNSLDKSIAKALLSIKAVFLRPEEPFIWASGIKSPIYCDNR
                     LTLSDVKVRREVEAGLAEIVKTHYPECEALMGTSTAGIAHAALTAEILGLPMGYVRSG
                     AKDHGRGNQIEGRLEKGQKVVVIEDLISTGGSVIEVVNVLRDAGAEVLGIASIFTYGM
                     QKGIDRLKEANVKNVSLSNLDALLEVAVETGYISETDKERIKKFRDNPADESWINA"
     gene            8201..8701
                     /gene="purE"
                     /locus_tag="PLD98_05315"
     CDS             8201..8701
                     /gene="purE"
                     /locus_tag="PLD98_05315"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQA05703.1"
                     /translation="MKKVAIIMGSDSDFPVVEPAIKVLQDYGVPFEVRVLSAHRCPDA
                     VRNYVKSARENGFGVIIAAAGKAAHLAGACAANTTLPVIGIPVKSSTLDGLDALLSTV
                     QMPSGVPVATVAIDGAANAAILAVQILSVFDETLAKQLMSNKLNSELEVLRKDKEIQK
                     KIDEIQ"
     gene            8717..9748
                     /gene="purM"
                     /locus_tag="PLD98_05320"
     CDS             8717..9748
                     /gene="purM"
                     /locus_tag="PLD98_05320"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HQA05704.1"
                     /translation="MKSHSESYKKAGVDITAGYRAVELIKKHIARTHIKGVASDIGGF
                     GGLFELDLSGYENPILVSGTDGVGTKLKLAFTLDKHDTIGIDCVAMCVNDIVCSGARP
                     LFFLDYLAVGKNYPEKIERIVAGVAEGCVQAGCALIGGETAEMPGFYRDGEYDLAGFA
                     VGIVDKKKMLDSSKVKVGDVIIGLPSSGIHSNGFSLVRKIIGDAGLDLTSYSQYTDSI
                     LGDALLAPTKIYVKPVLSLIEKVNVKSVAHITGGGFYENIPRALPDGIMAKIEKSVIK
                     VPPIFKLLQEEGAVPERDMFNIFNMGIGMCVIVAKKDADKALEILKEAGEDAYIIGET
                     VKGSGVDLC"
     gene            9742..10362
                     /gene="purN"
                     /locus_tag="PLD98_05325"
     CDS             9742..10362
                     /gene="purN"
                     /locus_tag="PLD98_05325"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQA05705.1"
                     /translation="MLNDNRRIKIAVLVSGGGTNLQALFDAEDKGILKSGEIVLVISD
                     RPEAYALERARKRNVEAVTIDRNAYDNAFYFETALIEALNKNEIELIILAGFLRILSE
                     RFIKQFENRIINVHPSLIPSFCGKGFYGLKVHKAALDYGVKVTGATVHFVNEIPDGGK
                     IIKQKAVEVLDGDTPETLQQRVMREAEWIILPEAAEEVCYKLKQKD"
     gene            10379..>10551
                     /locus_tag="PLD98_05330"
     CDS             10379..>10551
                     /locus_tag="PLD98_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HQA05706.1"
                     /translation="MEKLKLKDLLPQNPYWGRGIILGLSEDRAYAVCAYFIMGRSVNS
                     RNRIFVAEGDNIN"
BASE COUNT         3088 a         2290 c         2512 g         2661 t
ORIGIN      
        1 atcggcgctg tgcctcccca tgacggccag gaggtcacga ttctcggcat tatctcgggc
       61 gttacaagga aaatcaccaa agacgataaa acaatggcct tctttaagct ggaggacatg
      121 gacggcacaa ttgaggtcat tgtctttgca aaaatttatg agcagtacgc gtcgctcctt
      181 gttgaggaca gcgttgttat ggtaacaggc aggctgtctg tgcgcgagga tgaaaagact
      241 aagattatct gcgacaagat tgtagacggc gaaactgtcg taagagcctg caggcaaagc
      301 agcgctccgc ctccgcccga atacactccc attttctcat ctctgaaaac tcccgaaata
      361 agtacggagc ctgccgcaaa cgggacaatt aaaggttccg aggagtctaa tacaacggtt
      421 cagcagaata aagagctttg catgcccaca aacggcaatg gcaacggcaa cggcggctgc
      481 agaaagggtc ttttcctcag actaagcagc aaaaccgccc ctgaatttat caaagcctca
      541 tcactggttg acatattcgc aggcgatttg ccagtttact attactttgc ggatactaag
      601 gtctatgtaa actcaggcaa aagagtgctc attaatccca cgatgctcag tgagctgaaa
      661 tacattttgg gcgaaaagaa tgtggtaatg cgctcataaa aaggggtgat tattttgtcc
      721 gctgctttaa agttcttcct tgtgtcagat ttgcatcttt atgcctcttg ctttgaggac
      781 gacaagcgac tattgaagcc cgaccaaaag tttattgctg aatcaggcgc cataattgat
      841 tcagctttta accttatggc taaaaacaag gatataaaca tagtgctgat tgcaggcgac
      901 cttacataca acggcgagct tgaaagccat aaggaaatga ttgcgaagct ttacaggcta
      961 aaaaacgcgg gcaaaagagt tttcgttatc actgctaccc acgatttcgg caaggagcct
     1021 tcagtcaaat acaaaggctc cgacagactt gccgagacca ctccgaggga gtatctcgct
     1081 aagctctatt acccatttgg aaggaatgaa gctatctcag aagacgaaga atccacctcc
     1141 tactgcgtaa agctcgcaga aagctacagg cttttgtgcc ttaatgacga cagcaacgga
     1201 aaaggcagaa acggtttttc ggataagaca ttaaagtgga ttttagctca gatagccgac
     1261 gcaagaaggg caggggacac aatttttgcc atgacccatc atcccgttct tccgccgtcg
     1321 ccgatttatg agctgattgc aaaaagggat atgctgaccg acggagacaa aatctcggac
     1381 ctttttgccg atgcaggact tgagtttatc tttacaggcc attctcatat gcagaatatc
     1441 tccgttaagc tctccaaaaa cggcaacagg ttttacgata ttaacacagg ctcccttgtg
     1501 ggctatccga atcccattag ggttgtcaca cttgacgatg aggcaatgag agtcgaaaca
     1561 atcaatgtca ccgatttcaa atgggacttt gacggagtcg agcctcaaga atatcttaaa
     1621 aacaacttca gcagtcttct taatgaggct ttggacgccg cggcaaacga cattgaaaag
     1681 ctggctcagc ttgccgtggg catcagcgta aaaccagaga gcatattaaa gctgagagtt
     1741 cccattactt tgttcggaag gtttattaat aaactcaccg taggtcagct cggaaagatg
     1801 ttttttatta gctccaagat tgataagtca atagaaaacc ttttagtaaa gaatgtaatc
     1861 atagatattt tccaaaacct gtactgcggc gatgagccct attatcccgg aactcctata
     1921 tacacagcgg ttgaagcgct ttttgacaga atactgctgc tgaccaaaag aagcaaatac
     1981 tacggcaaaa taaaagagat tctctacacc ataaaagacg gagttctata tgacgctccg
     2041 ccttcggata acaatgcagt tcttccgagg cagaacaggc ctctcaaaga agccgaaagg
     2101 atgataattt gagtactaaa actgacagta tcacacacaa ggaaaataat gcggagttta
     2161 agccttttaa ggggtttttt aaacgcctga ttttcgcgtt tattataggc gttgcattta
     2221 tcacccccgg gctcagcggg ggagtcattg ctgccgccgc gggaatctat gagccttctg
     2281 tcaacgcaat tgtcacaata cataaaaact tcaggaaaag ctttttctac cttctgccca
     2341 tcggcatagg agccgtttcg ggcgtgctta ttttcagcag aattcttgag cagattattc
     2401 aatcttacga aacgaccctg atttttatct tcctgggtct tgttatcggc agtatccccg
     2461 cgtttattaa ggaagcaaat tccgagggct ttaaaaagat ttatgcgata cccaccgttc
     2521 taacctttgc ccttgttatg gttattgaga aaattctctc tcagcccgct ttccataatg
     2581 cggccgccga gcttactctc ccgatgttca ttgtctgcgg aattataatg tctctcggca
     2641 taattatccc cggcatcagc tcatctttta tacttatggc tatgggaatt tacaacgggc
     2701 tgttgtctac gatcaattcc tgggtggaca atgttttttc cttcataaaa ggcggtttta
     2761 aaggctttgg agctttcctt gcatcctttg actgggcttc gatttttgct ctgggtttga
     2821 gctttgccat aacctctata atcgcaataa agctcgttga catactcttt aagcgctatc
     2881 acggcgttgc ttattattcg gttttcggct ttttgctcgg ctctatggtc gcggcattcc
     2941 cgggctttag aggcggaatc ggcaccgcaa ttgacatagc cgccatggct gccgctgtgg
     3001 caatcggcta ctttggaatg aagctaaacg gaaaggtaaa agattaatgg gacgctttga
     3061 acatatattc ttcgatttgg acggcactct taccgacccc gctgagggaa tcactaattc
     3121 aatagcttat gtgctggatt attataaaat agattatcct tcaaaaaagg ctctggagcg
     3181 acatatcggt ccgccgcttc ataaaacctt tattaccgaa tacggttttg atgaaaactc
     3241 cgcggagctt gcaattcaaa aatacaggga atacttttcc gataaaggta tctttgaaaa
     3301 caggcttttt gacggaattc ccgaaatgct ctcaggttta aaagctaaat ctaaaaagat
     3361 ttatcttgcc acaagcaagc cccagtatta cgccaaaaag attttggagc attttgatat
     3421 cgcaaaatac tttgattttg tttcgggctg tgagcttgac ggcacgcgct cgcaaaaaac
     3481 agaggttata gagcatatct gtgaaaatgt cggcttaaag gatttgtctt ccagcgttat
     3541 ggttggcgac agaaaatact atattgaagg cgctcatcat tttggcatgc cctgcatagc
     3601 ggttttattc ggttacggca gcatggaaga gtttaaggag gctaaagccg actatattgt
     3661 ggagtcggtt gaagaactgt acaatttgtt gattagttaa cttattctcg aattttgtcg
     3721 atttttattc agcttaatag gtaagaatat acaaaattaa aaataattgt ccgataaaat
     3781 catagattta cacatcttga ttttgtttga caattatttt acttgttgta taatttatac
     3841 aggcggtgat tcagggggga gagcgttgaa ccttgttctt gcgaacggtt tcgtttttgc
     3901 agatgggggt tttgtaaaaa gcaatgttcg aatctcaggc ggcaaaataa tttcactcgg
     3961 catccaaaca tgcgagtttg cggatgctga gataattgac tgttcaaata tgtatataat
     4021 tcccggcttt gtcgatgtgc atgtgcatct acgagagccg ggattttctt acaaagagac
     4081 aattgcaagc ggcaccgcgg cggctgcaaa agggggatac accgccttat gccttatgcc
     4141 gaatcttaat ccagtgcccg acaccatgga aaatattaag ctgcagcttg atattataag
     4201 caaagacgcc cttgtaaaat gctatcccta cgcctcaatt acgaagggca gaacaggcag
     4261 aggcgaaatt accgacttcc acgagcttaa aaagtacgcc tgcgcgttta ccgatgacgg
     4321 aacgggcgta aatgaccctg aaactctcga gctggtagca aagaccgcaa aggctgagaa
     4381 tattatcatc gccgcccact gcgaggacga gactttggta aacggcggct atattcacgc
     4441 tggagaatac gccaaaagga acggtcataa gggtattgta tcggagagtg aatggctgcc
     4501 actaaaaaga gatatagaat acgccgaaaa gtacggcttt cattatcatg tctgccatgt
     4561 ttccacaaaa gaaagcgttg agctaatcag acaggcaaag aaaaggggag tcagaataac
     4621 agctgaaacc gcgccccact accttgtttt cactgatgag gatttagagg acgacggcag
     4681 attcaagatg aatcctccta tccgctctgc cgaggacaga aaagctctta tagaaggaat
     4741 aatcgacgga acgattgata taatcgctac cgaccatgcc ccccacagcg cagaggagaa
     4801 atctaagggt ctaaaggaca gccttatggg tatagtcggt cttgaaaccg ccttccccgt
     4861 tatgtacaca aagtttgtta aagaggaaat catctccctt gaaaaactga tagaaatcat
     4921 ggcaataaag cctcgcaaga ttttcagatt acctgattca tcgattaacg gcattgctga
     4981 gggtcaggcg gcggatattg cggtgcttga tttgaataca gaatacaaaa tcaacccaga
     5041 tacctttaaa agcaagggac gcagcacacc atttgcgggc atgactgtaa acggcgagtg
     5101 cgtaatgacc cttgtggacg gcaaaattgt atataaaaaa gcagataaac ggtgaagtaa
     5161 tgaggcagtt attgtttgaa gcagagaata tccgcgcaat agcggacaag gtatatgaac
     5221 tgaagctaac aggggatacc acggaaattg tccgccccgg tcagtttgta aatataagag
     5281 taataggcaa gtatttaaga cgccccattt cggtttgcga ctgggacagc gagagtttaa
     5341 ttcttatatt taaggttttc ggtgaaggaa cggagattct gagccgaatt aaaaaaggcg
     5401 aaaagatgga catcctcagc ggtcttggca acggctttac ggtgatagaa actgataagc
     5461 ctttgcttgt aggcggcgga gtaggtgttc cgccccttta cggacttgca aaaaagctga
     5521 ttgaaaacgg ccttaaacca aatgtcgttc ttggctttgc aagcgcaaag gatgttttct
     5581 ataaagatga gttcgaatct ctcggactta aagcctatgt agcaaccgtt gacggcacta
     5641 tgggaacaaa gggttttgtg accgacgcca taaaagagaa caatatcgaa tgtgattact
     5701 tttatgcctg cggtccgaat cccatgctaa aggctctttg ctctgagctt gaatgttcgg
     5761 gacagctcag tctcgaagaa agaatgggct gcggttttgg cgcatgcatg ggctgcagta
     5821 taaatacagc aaaaggaatc aaaagagttt gccacgacgg tccagtgttc tttaaggagg
     5881 atttactatg gaagtaaata tgaaagtaaa tctttccggc gttgagctcg acaatcccat
     5941 tatccccgcc agcggcacat tcggattcgg cgaagaattt aaggacctct atgacctaaa
     6001 tatcttaggc tcaatttcct ttaagggcac aacaagggag ccccgtttcg gaaaccccac
     6061 ccccagaatt gccgaatgcc cttctggact tttaaattcc gtaggtcttc aaaatccggg
     6121 cattgacgcg gtaatcaatg aggaaatacc taagctcagc aagtacttcc ataagccgct
     6181 tatagccaat atttcgggct tttcaataga agactacgca ttcgcctgcg aaaagataga
     6241 caaggttgag caggttggaa tcattgaagt taatataagc tgccccaatg taaagcacgg
     6301 aggaatgagc tttggcacca gccccgaaag cgctgcagag gttacaaaag ctgtaaaaga
     6361 agtcaccaca aagcccgttt atatcaagct cagccccaat gtaaccgaca tagccgcaat
     6421 agcgagagcc tgcgaggcag ctggcgcgga cggattctca cttataaaca cccttctcgg
     6481 tatgcgaatt gacattaaga aaagaaagcc cgtcctcgca aatgtggtcg gaggtctttc
     6541 gggtcccgca gttttccccg tagctttaag gatggtctat caggtctaca aggcagtcag
     6601 cgttccgatt atcggcatgg gcggcatttc gagcgcgtcg gatgttttcg aaatgatgat
     6661 ggcgggagcc tccgccgtac aaatcggctc cgcaaacctt gttgacccct ttatctgtaa
     6721 aaaaattatt gaagaactgc caattaaaat gcaagagctt ggaatcagct ccctttccga
     6781 attaaaaccc gatttttaga ggtgcgtatg aaaagagatg taattattgc ctgtgacttt
     6841 aacagcgcgg aggctgttta cagcttcctt gacaagttta ccgatgtaaa gccctttgta
     6901 aaaatcggca tggagctttt ttatgcagag ggtcccgcaa ttgttcgtga aatcaaaaag
     6961 cgcggatata aaattttcct tgacttaaag ctgcacgata ttcccacaac cgttaaaaag
     7021 agcatggctg ttctgtctgc tctcgatgtg gatatagtaa atcttcacgc cgctggcaca
     7081 tctgagatga tgaaggcagc tcttgagggg cttacccgcc ccgacggcac aagacccctc
     7141 ttaatcgccg ttactcagct tacttccacc agcgaggagc gtatgcagaa ggagcttttg
     7201 attaacgcca ctatggaaga aactgtggct cactatgctc tcaacgccaa aaactcgggt
     7261 ctggacggcg ttgtatgctc gccacttgaa gccggcatag tagcaaaagc ctgcggtgag
     7321 gattttataa gggtcacccc cggcatcaga tttgccgacg gcgatgtggg cgaccaggtt
     7381 agggtaacaa ctcccgcaaa ggctaaagag cttggcgcga catttatagt tgtgggcaga
     7441 cccatcacag ccgccgatga ccctgttgca gcatacaaaa gatgcgtaga agaatttgta
     7501 aattaatgta aattaattgg aggaagacat ggcaaactac aattctctcg ataaatcaat
     7561 agctaaggca ctgctttcaa ttaaggctgt tttcctgcgc cccgaagagc ctttcatctg
     7621 ggcaagcgga ataaaaagcc ccatttactg cgacaacagg ctgacacttt ccgatgtaaa
     7681 agtcagaagg gaagtcgagg cgggtcttgc cgagatagtg aaaactcatt atcccgaatg
     7741 cgaggccctt atgggcacct ccaccgcggg aattgcacac gccgctttaa ctgccgagat
     7801 tttgggtctc cccatgggct atgtgcgctc aggtgcaaaa gaccacggca ggggaaatca
     7861 gattgagggc agacttgaaa aaggtcagaa ggtagtcgta atagaggatt tgatttctac
     7921 aggcggcagc gtaattgagg ttgtaaatgt tctcagagac gcgggcgctg aggtgcttgg
     7981 catcgcaagc attttcactt acggtatgca aaagggcatt gaccgtctta aagaggcaaa
     8041 tgtgaagaat gtttctcttt ctaaccttga cgctctgctt gaggttgcag tagaaaccgg
     8101 ctatattagc gaaaccgaca aagagagaat aaaaaagttc agggacaacc ctgctgatga
     8161 aagctggatt aacgcttaac ctaaaaattg gggtgtgaat atgaaaaagg tagcaattat
     8221 tatgggaagc gactcggatt tcccagtagt agagcctgca attaaggttc ttcaggacta
     8281 tggcgtaccc tttgaagtaa gggtactgtc cgcccacaga tgtcccgacg cagtaagaaa
     8341 ttatgttaaa tccgcaagag aaaacggttt tggcgtaatc atcgccgcag cgggaaaggc
     8401 tgctcatctt gcgggagcct gcgccgctaa cacaactttg cccgtaatag gcattcccgt
     8461 taaatcctcc acacttgacg ggcttgacgc gctgttgtcg accgtacaaa tgccttcggg
     8521 cgttccggtc gcaaccgttg caattgacgg cgccgcaaat gccgcaattt tggctgtgca
     8581 gattctttcg gtttttgatg aaactctcgc aaaacaactg atgagcaata aactcaacag
     8641 cgagcttgag gttctgagaa aggataagga aattcaaaag aaaatagacg aaatacaatg
     8701 aaaggacagg agttaaatga agagccacag cgagtcttac aaaaaagcgg gcgtagacat
     8761 taccgctggc taccgcgccg tagagcttat aaaaaagcat attgcaagaa ctcacattaa
     8821 gggcgttgcc tcggatatag gcggctttgg cggcctgttt gagcttgatt taagcggata
     8881 cgaaaatccc atcctcgtat caggcaccga cggtgtaggc acaaagctta agctcgcctt
     8941 tactcttgat aagcacgaca ccataggcat agactgcgtt gccatgtgcg taaacgacat
     9001 tgtatgctct ggcgcgagac cattgttctt ccttgactac ttggcagtag gaaaaaacta
     9061 tcccgaaaaa attgaacgaa tcgtagcagg agtagcagag ggctgcgttc aggcaggctg
     9121 cgctctgatt ggcggcgaaa cagcggagat gcccggcttt taccgtgacg gcgaatatga
     9181 ccttgcgggc tttgccgtcg gaattgttga caaaaagaag atgctagaca gctcaaaagt
     9241 caaagtcggt gatgtaataa tcggacttcc ttcatcggga atccactcca acggcttctc
     9301 actcgtcaga aaaattatcg gagatgccgg tctagactta acctcctatt cccagtacac
     9361 cgacagcatt ttgggcgacg cgcttttggc tcccacaaag atatatgtaa aacccgtttt
     9421 aagccttatc gaaaaggtta atgtaaagtc tgtggctcac attacaggcg gcggatttta
     9481 tgagaatatt cccagagctt tgcccgacgg cattatggca aagatagaaa agtctgttat
     9541 aaaagtcccg ccgatattca aacttcttca ggaggagggc gcagtgcccg aaagggatat
     9601 gttcaatata ttcaatatgg gcattggcat gtgcgtgatt gtagcaaaga aagacgccga
     9661 caaagccctc gaaatcctta aagaagcagg ggaggacgca tacataatcg gcgaaaccgt
     9721 aaaaggtagc ggagttgacc tatgctgaac gataacagaa gaataaagat tgccgtcttg
     9781 gtatcgggcg gcggaacaaa tcttcaagct ctgtttgacg ctgaagataa gggtattctg
     9841 aaatccggcg aaatagtgct tgtcatctcc gacagacccg aagcctacgc tcttgaaaga
     9901 gcaagaaagc gtaatgtaga agcggtgaca attgaccgaa acgcatatga caatgctttc
     9961 tattttgaaa ccgctttaat cgaagcatta aataaaaacg aaatagagct tattatcctt
    10021 gcgggttttt tgcgcatact ctcagagcga tttataaagc agtttgaaaa ccgaataatc
    10081 aatgttcatc cctcccttat tccttcattt tgcggcaagg gattctacgg tcttaaagtt
    10141 cataaagccg ctttggacta cggagtgaag gtaacgggcg cgacagtgca ttttgtgaac
    10201 gaaatccccg acggaggtaa aatcatcaaa caaaaggctg ttgaagtgct tgatggcgac
    10261 actcccgaaa ccctgcagca aagagtaatg agggaagcgg aatggattat tttacccgag
    10321 gccgcggaag aagtgtgcta caaattaaaa caaaaagact gataggaagg caaaagagat
    10381 ggaaaagctt aaactaaaag atcttttacc tcaaaaccca tattggggca gaggaattat
    10441 tttgggcctc agcgaagaca gagcatatgc cgtttgcgca tatttcatta tgggcagaag
    10501 cgtaaacagc agaaacagaa tctttgtcgc tgaaggcgat aatatcaata t
//
LOCUS       DAONVQ010000064        10526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4853_length_10526_cov_3.708719, whole genome shotgun sequence.
ACCESSION   DAONVQ010000064 DAONVQ010000000
VERSION     DAONVQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10526
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4853_length_10526_cov_3.708719"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="PLD98_05335"
     CDS             <1..1825
                     /locus_tag="PLD98_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05707.1"
                     /translation="KCAVCSASYTQDYDKTDHSLEPIPAVAPTCIETGLTEGSRCGMC
                     GEVFVEQETVPALGHSPVLDPAIEPTCDTNGLTEGSHCSVCGEILIKQESIPALGHTF
                     TWEYDGDIHTGTCSVCGGVESHIGDYEEIETPYTGTIDGCATFKCKYCDHTYTEDYVA
                     KAGNTLYTSLEAALQAANSGDKVILLKDYTMTRDAEVKSGVLLLIPCMDNDIGYTSSG
                     YNPDGTTTANYTKEDVLYRTLTIADGAEITVNGTMLINAVTGRKVAGSYTTYDITGGY
                     ALVNLDGDIVVNSSGVLDCSGRVEGDGQVTLKSGATMYETYSIVRWRGGSYFKNHTFD
                     KKRVYPIYESEMNTMRAALRIESGASLYGTVKVYTSEYNLGGFIVIPASYHYHRFLII
                     GSGSTTGSANGLYRLHDDAYCIRTVEWDDSLNTKFNTTITPGGYRDVYKFFGGMSFCA
                     QSLMLNIGGFNQTLLSDRCGYFTVDGDARYEFYDGEYNLLRKFEFLPGFQMYAGNGAV
                     INVQSTGGLLFADSGFYTSDSKFTNVGRYPSGRADAYLQLSDGAVLNVASGGEIAGKV
                     IYSPDCTININSSAKTSVSTNVPTSASAYEVYTAVYQPILN"
     gene            complement(1947..3386)
                     /gene="proS"
                     /locus_tag="PLD98_05340"
     CDS             complement(1947..3386)
                     /gene="proS"
                     /locus_tag="PLD98_05340"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQA05708.1"
                     /translation="MADNKKMVESITPMDTDFAKWYTDIVKKAELIEYSSVKGCMIIR
                     PYGYAIWELIQKDLDRRFKETGHENVYMPMFIPESLLQKEKDHVEGFAPEVAWVTHGG
                     QEELAERLCVRPTSETLFCEHYANIIQSYRDLPKLYNQWCSVVRWEKTTRPFLRSREF
                     LWQEGHTAHATPEEAIAETERMLNVYADFCKETLCMPVVKGRKTDSDKFAGAEMTYAI
                     EALMHDGKALQAGTSHYFGDGFARAFGITFADKDNKLSYVYQTSWGVTTRLIGGIIMT
                     HGDDNGLVLPPAVAPIQVIIVPIAQHKPGVLEKANEIAEQLKKKFRVKLDDSDNTPGW
                     KFAEYEMKGVPLRLEIGPKDIENNQCVLVRRDNREKYFVPFDNLESQIEEILKVYAEN
                     IYNKAYENMQKRTYDCKSIDEIKQVLAEKGEGFIKAMWCGDEACEDSVKEQTGVASRC
                     IPFEQERISDKCVCCGKEAKHLLYWGIAY"
     gene            complement(3513..3734)
                     /locus_tag="PLD98_05345"
     CDS             complement(3513..3734)
                     /locus_tag="PLD98_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQA05709.1"
                     /translation="MIYEKVVSIICDQLGLDEEDITLDSVLMDIVDDSIDLVELVKAF
                     EDEFNIVVPDEEFENFTTVADVVKYLEEN"
     gene            complement(3830..5239)
                     /locus_tag="PLD98_05350"
     CDS             complement(3830..5239)
                     /locus_tag="PLD98_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQA05710.1"
                     /translation="MSVKTLTKRFYDPHYLIKGQWKGNRISAKEAYTRLYRIAWASTL
                     ETLLISMISFIDTVMVSTLGHEAVAAVGLTTQPRNIFFAVFSALNVGVTAVVSRRRGE
                     ENREAANKTMSQAVFLSSILGVILFVVSYYLSEDFLLFMGAQDDTIHLAVPYFRIIML
                     GTVFTSVGLMINAAHRGCGNTKISMVTNLTANLVNIVFNYLLINGVWFFPKLGVTGAA
                     IATLIGNILSFIMSVYSVTVRPGYLHLQLSHIFKLSKDILSPITKVSSGAAVEQLFVR
                     IGFFVYAKLVATLGTESMATHQICMSIINLSFSLGDGLGIAASSLVGRTLGQKRSDIS
                     LVYGKATQRVGFVLGMVLSLIFIFGRYGIMSLFSDSESIISLGAQIMIIVSFIAPAQI
                     SQVIFTGSLKGAGDTRFIAVSSLVSIAIIRPLITYILCYIVGMGLIGAWLSLAIDQLI
                     RLTAVGLRFSSGKWKSIKL"
     gene            complement(5267..6106)
                     /gene="nadC"
                     /locus_tag="PLD98_05355"
     CDS             complement(5267..6106)
                     /gene="nadC"
                     /locus_tag="PLD98_05355"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HQA05711.1"
                     /translation="MILSQHYIDNLIYKALDEDINYIDISSAYIFTDDMKSTAYFVSK
                     ADGVLAGIDVALRVFEMLDKDVVINKICKDGDKITKGTEIATIEGKTAMLLKGERTAL
                     NILQHMSGIATAVSKAVEAVSHTNATIADTRKTLPGLRPLQKYAVTCGGGKNHRYNLS
                     DAVMLKDNHIDAGGGISKTVKTVRQHIGHTVTIEVETRNLAEVKEALDAGADIIMLDN
                     MTVDEMKEAVKLIGGRAKTEASGNITLDNIKEVAETGVDIISMGAITHSVKAFDISMK
                     IRN"
     gene            complement(6103..7578)
                     /locus_tag="PLD98_05360"
     CDS             complement(6103..7578)
                     /locus_tag="PLD98_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQA05712.1"
                     /translation="MQSKRQLKKTIYDVLIVGSGVAGMYGALHFDDDTDVLLLSKYGK
                     TVSNSNLAQGGVAAVLSFKDDSFEQHIEDTMIAGLRCNDIEAVDILVREGPNDVRRLI
                     EWGVNFDKNENGELDLTLEGGHSRRRILHYKDSTGAEIVRGLLKKVEEKENIELAENA
                     YLCDLTKVKSGFRADILINGSYESIFCSYCILCTGGIGRVYQYTTNPKTATGDGIRIA
                     YELGARIKNLSYIQFHPTAFNSDDGERFLISESVRGEGAYLLNCNLERFMHRYDDRLE
                     LAPRDVVSKSIILESRRTGSNEFYLDIRHKSKEYLMERFPAIYAGCLKYGVDISKDLI
                     PVFPCQHYLMGGIEVDLDSKTTVPRLYAAGECANTGVHGKNRLASNSLLEALVFSRRA
                     AQDIKRYMANGTPKVTEHSFNVDYTGAPLPPGTADEIRSIMQRSYFVLPNPDKIRTGL
                     QQAENILKRLKSAKFAKTIDYLEALSLATVATIILSEVENL"
     gene            complement(7611..8135)
                     /locus_tag="PLD98_05365"
     CDS             complement(7611..8135)
                     /locus_tag="PLD98_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05713.1"
                     /translation="MKQNSKIALGGIISALSLALMLMTSILPFLTYALPIVSGALLML
                     IVIEIGKKWAVYVYITVSIISLFVLSDKEAAVVYLMFFGIYPILKAIIEKRFHAAFEY
                     FLKFLIFNSSMILSYAIIVYIFKLPVETMTKTYLIFVLLELNVLFFMYDLMLTKLITA
                     YLKKWRKSFLKLFK"
     gene            complement(8157..8759)
                     /locus_tag="PLD98_05370"
     CDS             complement(8157..8759)
                     /locus_tag="PLD98_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HQA05714.1"
                     /translation="MKRNPYEVLGISESASSEELKNAYIYLARKFEEDKYADPPLCDI
                     AKQKLEELNWAYDEIIRLRSSNSSYYASGNQYQSYGSNNQYTDIYRLLDEGKYYEAES
                     RLDAIPEYLRDAEWYYLRGKTYYFLGWYDSAENAYKTAHKKDKSNPKYKVAYENMRGS
                     RDIPYKESDSSSCDAGDLCCSLLCADCCCECMGDDLIPCC"
     gene            complement(8740..9633)
                     /locus_tag="PLD98_05375"
     CDS             complement(8740..9633)
                     /locus_tag="PLD98_05375"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HQA05715.1"
                     /translation="MFGYVRINKAELKFKDYEAYRAVYCSLCRELGKRYSVFARAFLN
                     YDLAFLALFSLSVSDSALNFKKGRCPFNPLKKCNFSAVSSAEISDAADATVIMSYYKL
                     KDNLADKGFKKKAAALIILPFALLSVRRARKLNPEIFEIIETAVKNQKEIEKSNAKDE
                     SRSIIDLSADSSAKAISQLIARRIEDDSQKNAASRFGYNIGRWVYLIDALDDLKDDIK
                     RGNFNPYCVCGNFNMPDLEKIRDKAKSDLIQTANQAILAYKQIEKKRFSAVLDNIVYD
                     GLFSEISRVYHGRKINEEKPL"
     gene            complement(9707..>10526)
                     /locus_tag="PLD98_05380"
     CDS             complement(9707..>10526)
                     /locus_tag="PLD98_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HQA05716.1"
                     /translation="SHGLDLAIVNPSSVVMMDTYYSYLALAGLDEHCEKYIERLSSRK
                     VEYINQSSPIQNTVIADDNESKNPLFKAIVNGEANSAYQQTKELLKNASSEYIVENIL
                     VKALDYVGKAFETKSIFLPQLMKSAESAKSAFNAIKDGLSGGAGENSKGDVLLATVKG
                     DIHDIGKNILKVMLENYGFNVIDLGKDVSPERILEEILQQNIKLVGLSALMTTTVESM
                     ASTIKLIKEKAPDCRIMVGGAVLTAEYAKEIGADFYGRDAMSGVIYAQRVFGEK"
BASE COUNT         2743 a         2445 c         2318 g         3020 t
ORIGIN      
        1 aaagtgcgcc gtatgctcag cgtcttatac acaagattat gataaaacag atcacagctt
       61 ggagcctatc cccgcagtcg ctccgacctg catagaaacg ggacttactg aaggctcccg
      121 ctgcggcatg tgcggtgagg ttttcgtaga gcaggaaaca gtccccgcgc tcggtcattc
      181 tcccgttttg gaccccgcta ttgagccgac ttgcgacact aacggactta ccgaaggcag
      241 tcactgctca gtatgcggtg agattcttat taagcaggag agtatccccg cactcggtca
      301 tacatttacc tgggagtacg acggcgatat acatacaggt acctgcagtg tctgcggcgg
      361 agtggagagt catataggag attatgaaga aatagaaact ccatacacag gtaccatcga
      421 cggctgcgca acctttaaat gcaaatactg cgaccatact tataccgaag actatgtagc
      481 caaagccgga aatactcttt atacaagcct tgaggcagct ctccaggctg caaatagcgg
      541 agataaggta attcttctta aagactatac catgacaaga gacgctgaag ttaaaagcgg
      601 tgttttgctt ttaatccctt gcatggataa tgacatcggc tatacttcat cgggctataa
      661 tcccgacgga acaaccacag ccaattacac caaagaagat gtgctttatc gaacgcttac
      721 aatagccgat ggtgccgaga ttaccgtaaa cggcactatg cttataaatg ccgtaaccgg
      781 aagaaaggtt gccggcagct acaccacata tgatattacc ggcggttatg cgcttgtaaa
      841 tcttgacggc gatattgtgg ttaatagctc aggagtgctt gactgctcgg gcagggtaga
      901 gggcgatggt caagtcacct taaagagcgg agctaccatg tatgaaactt attcaatagt
      961 cagatggcgc ggcggctcat attttaagaa tcatactttt gataaaaaaa gagtctatcc
     1021 gatttacgag agcgaaatga acacaatgcg cgccgcttta agaattgaaa gcggagcttc
     1081 gctttacgga actgtaaagg tatatacaag cgaatacaac cttggtggct ttattgtaat
     1141 tcctgcatca tatcattatc atcgcttcct gattatcggc agcggcagca caacgggcag
     1201 cgcgaacgga ttatacaggc tccacgacga tgcttactgc atcagaacag ttgagtggga
     1261 cgacagcctg aataccaaat tcaataccac aattacgccg ggcggatatc gtgatgtata
     1321 taagttcttc ggcggtatgt ctttctgcgc acaatctctt atgctgaata tcggcggctt
     1381 taatcaaacc ctgctcagcg accgatgcgg atacttcacc gttgacggtg atgcccgata
     1441 tgagttctat gacggcgagt acaacctttt aaggaaattt gagttcctgc ccggattcca
     1501 gatgtatgcg ggaaacggcg ctgtcatcaa tgtccagagc acaggcggac tattatttgc
     1561 cgacagcggt ttctatacca gtgattccaa atttaccaat gttggaaggt atccttcagg
     1621 cagagcggac gcttatctgc agctttccga cggagcagta ctgaatgtag cctcgggcgg
     1681 agaaattgct ggcaaggtaa tatacagccc cgattgtact attaatatta attcgtccgc
     1741 caagacaagc gtaagcacaa atgtcccgac aagcgcatcg gcttatgaag tttataccgc
     1801 tgtataccag cctatattaa actaagcctt ttgttgggaa cctaatgctc ctttttgtcc
     1861 tcctttttag aaagaccgcc ttttattaag gcggtctttc ccttcaaaaa agggcaggct
     1921 ttttgcctgc ccttaaattc tttggtttaa taagcaatac cccaatagag aaggtgcttt
     1981 gcctctttgc cgcagcatac gcatttgtcg gagattcgct cctgctcgaa gggtatgcat
     2041 cttgaagcga cgcctgtctg ctccttaacg gagtcctcgc aagcctcatc gccgcaccac
     2101 attgccttaa taaagccttc gcccttttcg gcgagaacct gcttaatctc gtcaatcgac
     2161 ttgcagtcat aggtgcgttt ctgcatgttc tcatatgcct tattgtaaat gttttcggca
     2221 tataccttga ggatttcttc aatttggctt tcaaggttat cgaagggtac aaagtatttt
     2281 tctctgttgt cgcgccttac aagtacgcac tgattgtttt ctatgtcctt cggaccgatt
     2341 tcaagtctga gcggtacacc cttcatctca tactcagcaa acttccagcc gggggtgttg
     2401 tcagagtcgt caagctttac tctgaatttc ttcttaagct gttcggcgat ttcgtttgct
     2461 ttttcgagta cgccgggctt gtgctgagca ataggaacaa tgataacctg aatcggggcg
     2521 actgcgggcg ggaggacaag accgttgtca tcgccgtgag tcatgattat gccgccgata
     2581 agccttgttg taacgcccca tgaagtctga tatacataag agagtttatt gtccttgtct
     2641 gcaaatgtaa tgccgaatgc ccttgcaaag ccgtcgccaa agtaatggga tgtgcctgcc
     2701 tgaagtgcct ttccgtcgtg cataagagcc tcgatcgcat aggtcatctc agcgcccgcg
     2761 aacttgtcgc tgtcggtctt tcttccctta acaacaggca tgcatagggt ttccttgcag
     2821 aaatcggcgt aaacattcag catcctctcg gtttcggcaa tagcctcttc gggagttgcg
     2881 tgggcggtgt gtccttcctg ccataaaaac tctcttgagc gcaggaaggg ccttgttgtc
     2941 ttttcccagc gaacaacgct gcaccactga ttgtagagtt tcggcaaatc tctgtaagat
     3001 tgaattatat ttgcataatg ctcgcagaaa agagtttcgg aggtgggtct tacgcaaagt
     3061 ctttcagcaa gctcctcctg tccgccgtgg gttacccatg cgacctcggg agcaaagcct
     3121 tcaacatggt ccttttcctt ctgcaaaagg ctttcgggaa taaacatagg catgtagaca
     3181 ttttcatgtc ccgtttcctt aaagcgtctg tcaaggtcct tttgaataag ctcccatata
     3241 gcatagccgt atggccttat aatcatgcag cccttaacgc ttgagtattc gataagctct
     3301 gcctttttaa caatatccgt gtaccatttg gcaaagtcgg tatccatggg agtgatactc
     3361 tcgaccattt tcttgttgtc tgccatcaga tagcctcctc tgaattacat aagcaataac
     3421 attatagcac taataaacta aattatataa taaaaaatcc caagccgcaa ggcaaaataa
     3481 aaggcgtttt taaacgcctg attgggattc ggttagtttt cctctaaata tttgacgaca
     3541 tccgcaacgg tggtgaagtt ttcaaattct tcgtcgggaa ccacgatatt gaactcatct
     3601 tcaaaagcct ttacaagctc aacgaggtcg atgctgtcgt caacgatatc cattaatact
     3661 gagtcgaggg tgatatcttc ttcgtcaaga cctaactgat cgcaaatgat tgaaacaacc
     3721 ttttcataaa tcatattatc gactcctaac tttcaccatt taatactaca aagcaatatt
     3781 agatagtttg agcgtttttg tcaatacctc aatggaaagg gaaatacatt tacagcttaa
     3841 ttgatttcca tttgccgctt gagaagcgaa gacccacagc ggtaaggcga atgagctggt
     3901 ctatggcaag actgagccat gctccgataa gacccattcc aacaatgtag caaaggatat
     3961 aggtaattag cggtcttatt attgcgatgc tgacaaggga agataccgca ataaaccttg
     4021 tatctccggc gcctttaagg ctgcccgtaa agataacctg cgaaatctgc gcgggagcaa
     4081 taaaggagac aataatcatt atttgcgcgc cgagcgatat tatcgattcg gaatcggaaa
     4141 aaagcgacat aatgccgtat cttccgaaaa taaaaatcaa agaaagcacc atgcctaaaa
     4201 caaagccgac cctttgagtc gctttgccgt aaaccagcga aatgtcgctg cgcttttggc
     4261 cgagggtcct gccaacaagg gaggatgcgg caattccgag tccgtctccg aggctaaacg
     4321 aaaggtttat tatgctcatg caaatctggt gtgttgccat gctctcagtg ccgagggtgg
     4381 cgaccagttt tgcgtaaaca aaaaagccga ttctgacaaa aagctgctcg accgctgcgc
     4441 cgcttgaaac ctttgtgata ggggagagta tatccttaga aagcttaaat atatggctaa
     4501 gctgcaaatg aagataaccg ggtcttaccg ttaccgagta aaccgacata atgaatgaca
     4561 ggatattgcc tatcagcgtc gcaattgccg ctcccgtaac gccgagtttt gggaaaaacc
     4621 aaacgccgtt aataagaagg tagttgaata ctatattaac caaatttgcg gtaaggttgg
     4681 tcaccattga aattttggtg ttcccgcagc ctctgtgcgc cgcgtttatc ataaggccaa
     4741 cggatgtgaa aacggtgccg agcattatga ttctgaaata gggcaccgca aggtgaattg
     4801 tatcatcctg cgcgcccatg aaaagaagaa aatcctcgct gagataataa gaaaccacaa
     4861 aaagaatgac acccaaaatc gaactcagga aaaccgcctg cgacatggtt ttgttcgcgg
     4921 cttctctgtt ttcctcgccc ctgcgcctgc tgacaacagc agtgacgccc acattcagcg
     4981 cggagaaaac agcaaagaag atattcctcg gctgagtagt tagaccaacg gcggcaaccg
     5041 cttcgtgtcc caaagtgctg accattacgg tatctataaa ggaaatcatg cttataagca
     5101 gtgtttccag agtagaggcc caggctattc tgtaaagcct tgtataagcc tcttttgcgc
     5161 ttattcggtt gcctttccac tgacctttaa taagatagtg ggggtcatag aaccttttcg
     5221 tgagtgtttt tacagacatt gctaacgcct ttcggtattt ttattatcag tttctaatct
     5281 tcatggaaat atcgaatgct ttgaccgaat gagtaatggc tcccatggag attatgtcaa
     5341 ctcccgtttc ggcaacttct tttatattgt caagggtgat atttcccgat gcttcggttt
     5401 ttgctctgcc gcctataagt tttacggctt ctttcatttc atcgacggtc atattgtcga
     5461 gcataataat atccgcaccc gcgtcaaggg cttcttttac ctctgcaaga ttgcgggttt
     5521 cgacctcgat tgtaacggta tggcctatat gctgtcttac ggtctttact gtttttgaaa
     5581 ttccgccgcc cgcgtcaatg tgattgtctt taagcataac tgcgtcggac aaattgtacc
     5641 tatggttctt gccgccgccg caggtaacgg catatttttg aaggggcctg agccccggca
     5701 gggttttcct tgtgtctgca attgtggcat tggtgtgaga aacagcctcg acagcctttg
     5761 aaactgccgt agctatgccc gacatgtgct gaaggatatt aagggcggtt ctctcgcctt
     5821 taagcagcat ggcggttttg ccttcaatag tcgctatttc ggttcccttt gttattttat
     5881 cgccgtcttt gcagatttta ttaatgacaa catccttgtc aagcatctca aataccctga
     5941 gagctacatc aatgcccgca agaacaccgt ccgcctttga aacaaaatag gcggttgact
     6001 tcatatcatc ggtgaaaata taggcgcttg atatatcaat atagtttatg tcttcatcta
     6061 aggctttgta aatcaggttg tctatatagt gctgagaaag aatcataagt tttcaacctc
     6121 gctaagaatt atggttgcca cggtggcaag gctgagcgcc tcaagataat ctatggtttt
     6181 tgcgaatttg gcgcttttaa gccgtttgag aatattctcc gcctgctgga gtcccgttct
     6241 gattttgtcg ggattgggga gcacgaaata agacctttgc attattgacc gtatttcatc
     6301 cgcagtgccg ggaggcagtg gtgcgccggt gtagtcgaca ttgaaggaat gctctgtaac
     6361 cttcggagtg ccgttcgcca tataccgctt tatatcctgc gcggctctgc gggagaaaac
     6421 cagtgcctcc aaaagggagt tgctcgcaag cctgtttttg ccgtggactc ctgtgtttgc
     6481 gcattcgcca gcggcataaa ggcggggaac ggttgtctta gaatcgaggt caacctcaat
     6541 tccgcccatt aggtaatgct ggcatgggaa tacgggaatc aaatcctttg atatgtcaac
     6601 cccatatttc aagcagcccg catagatggc ggggaaacgc tccatcaagt attccttaga
     6661 tttatgccta atatcaagat agaactcgtt gctgcctgtc cttctgcttt caagaataat
     6721 ggactttgaa accacatctc tcggtgcaag ctccagcctg tcgtcatacc tgtgcataaa
     6781 gcgctcaaga ttgcagttta aaagatatgc gccctctccc ctgaccgact cgctgatgag
     6841 aaacctttcg ccgtcgtctg agttaaaggc ggtggggtgg aactgaatgt agcttaaatt
     6901 ctttattctg gcacccagct cataggcaat tctgatgccg tcgccggttg cggttttggg
     6961 gttcgtggtg tactgataga ccctgcctat tccgccggtg cagagaatac agtagctgca
     7021 gaaaatgctc tcatatgagc cgtttataag aatatcggca cgaaagccgc tttttacctt
     7081 agtcaaatcg cagagataag cgttttcggc aagctcaatg ttttcctttt cttcgacctt
     7141 tttcaataga ccccttacta tttcagcgcc cgtgctgtcc ttatagtgga gaattcgcct
     7201 tctggaatga ccgccctcca aggtaaggtc aagctcgccg ttttcatttt tatcaaagtt
     7261 tacgccccac tcaatgagcc gtcttacatc attgggcccc tctcttacaa gtatgtcaac
     7321 ggcctcaatg tcattgcacc taagacccgc aatcattgtg tcctcaatgt gctgctcaaa
     7381 gctgtcatcc ttaaaggata aaacagctgc aaccccgcct tgggcgagat tgctgttgga
     7441 gaccgttttt ccgtattttg aaagaagtaa tacatcagtg tcgtcatcaa aatgcaaagc
     7501 accgtacatt cctgcgacgc cgctgccgac tattaaaaca tcataaattg tcttttttaa
     7561 ctgtcgttta ctttgcataa agcacactct ttcaatacaa aatgtaccga ttatttaaat
     7621 agcttcaaaa agctctttct ccacttttta agatatgccg ttatcagttt tgtaagcata
     7681 aggtcgtaca taaagaaaag gacattaagc tctaaaagga caaatatcaa ataagtctta
     7741 gtcatagttt cgacaggaag tttaaaaata tatactatta tagcataaga gagaatcatt
     7801 gaagaattaa atatcagaaa tttaagaaaa tactcgaaag ccgcgtggaa tctcttttct
     7861 ataatcgctt tcagtattgg ataaattccg aagaacatta agtacacaac cgctgcctct
     7921 ttgtcggaga gcacaaacag cgaaattatg ctgaccgtaa tataaacata cactgcccac
     7981 tttttgccta tttctatgac aataagcatt aaaagcgcac ccgacacaat gggcagggca
     8041 taggttaaaa aggggagtat ggaggtcatc agcattagtg caagggaaag ggcagagata
     8101 atacccccca gcgcgatttt tgagttttgc ttcatttaat ttgcctccga gaatatttag
     8161 cagcagggta ttaggtcgtc gcccatgcat tcgcagcagc agtcggcgca caaaagagag
     8221 cagcataaat cgcccgcgtc gcatgaggag gagtctgatt ccttgtaggg aatatccctt
     8281 gagcctctca tgttctcata tgccacttta tatttaggat tgcttttgtc ctttttgtgg
     8341 gcggttttgt acgcgttctc cgcagagtca taccagccga ggaaataata ggttttgccc
     8401 ctgagataat accactcggc gtcccttaaa tactccggaa ttgcgtcaag gcggctttcg
     8461 gcttcgtaat acttgccttc gtcaagcagc ctatatatgt cggtatattg attatttgaa
     8521 ccgtagcttt gatactgatt gccgctagcg tagtatgagg aattggagct tcgcagtctt
     8581 atgatttcat cataagccca gtttaattct tccagctttt gctttgcgat atcgcatagc
     8641 ggtgggtctg cgtacttatc ttcctcgaac tttctcgcaa ggtatatgta cgcgttttta
     8701 agctcttcgc tgcttgcgga ttcggatatg ccgagcactt cataggggtt tctcttcatt
     8761 gattttcctc ccgtgatata ctcttgagat ttcagaaaac agaccgtcgt aaactatatt
     8821 gtccagcacc gccgaaaagc gctttttttc aatctgctta taagcgagta ttgcctgatt
     8881 tgctgtttga atcaaatcgc ttttagcctt gtctcttatc ttttccaaat cgggcatatt
     8941 gaaattgccg catacgcagt aagggttaaa gttccctctt ttaatatcgt cctttaaatc
     9001 gtcaagggcg tcaatgagat atacccatct gcctatgtta tatccaaagc gtgacgccgc
     9061 attcttctgc gaatcatctt caatgcgtcg ggcaataagt tgtgagattg cttttgccga
     9121 tgaatcggcg gacaagtcta taatgctgcg gctctcatct tttgcgttag acttttcaat
     9181 ttccttttga ttttttaccg cagtttcaat tatctcaaag atttcgggat ttagcttcct
     9241 tgctcttctc accgaaagca aagcaaatgg cagtattatt aacgctgccg cttttttctt
     9301 aaatcccttg tccgctaaat tgtcctttaa tttatagtat gacattatga cggtcgcatc
     9361 ggccgcgtct gaaatctctg cggaggaaac agcgctgaaa ttgcactttt taaggggatt
     9421 gaaggggcat ctgccttttt tgaagttaag agcagagtcg gaaacagaga ggctgaacaa
     9481 cgctaaaaag gctaaatcgt agtttaaaaa ggctcttgcg aaaactgagt agcgttttcc
     9541 aagctccctg caaagagagc agtaaaccgc cctgtaggcc tcataatctt tgaatttcag
     9601 ttcagccttg tttatgcgca catacccgaa caatctgctt cctccaatag ctttgagcct
     9661 attgtatcac aaaaaacact aataggctat ttttgacaaa atattatcac ttttcgccga
     9721 aaactctctg agcgtatatt acgcctgaca ttgcgtccct gccgtaaaag tccgcgccga
     9781 tttcctttgc gtattccgct gtcaaaacag ctccgccgac cataattctg caatcgggtg
     9841 ctttttcctt aatcagctta atcgtacttg ccatgctttc aaccgttgta gtcattagag
     9901 cgctgagtcc caccagcttt atgttttgct gcaggatttc ctccaaaatt ctctcggggc
     9961 ttacatcttt accaaggtca atgacattaa aaccgtagtt ttcgagcatg acctttaaga
    10021 tgttctttcc gatgtcgtga atatcgcctt taaccgtcgc aagcaggaca tcgccctttg
    10081 agttttctcc tgcgccgccg gacaagccat cttttattgc gttgaacgct gatttggcag
    10141 actccgcgga cttcataagc tggggcagga agatactctt tgtctcaaac gccttgccca
    10201 catagtccag agcttttaca aggatatttt caacaatata ctctgatgaa gcatttttaa
    10261 gcagctcttt tgtctgctga taagcagaat ttgcctctcc gtttacaata gccttaaaga
    10321 gaggattttt tgattcatta tcatccgcaa tcactgtgtt ttgtatgggg ctgctttggt
    10381 ttatgtactc gacctttctg gagctgagcc tttcaatata tttttcgcaa tgctcatcaa
    10441 gccccgccaa agcaaggtag gagtagtaag tatccatcat gaccacgctt gaggggttga
    10501 cgattgcaag gtcgagacca tgactt
//
LOCUS       DAONVQ010000065        10515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4861_length_10515_cov_4.899426, whole genome shotgun sequence.
ACCESSION   DAONVQ010000065 DAONVQ010000000
VERSION     DAONVQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4861_length_10515_cov_4.899426"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3072)
                     /locus_tag="PLD98_05385"
     CDS             complement(<1..3072)
                     /locus_tag="PLD98_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05717.1"
                     /translation="MLYPKSREKELKEDLFIKPSSEYRGAPFWSWNCKLTNELLEKEI
                     KDLKDMGFGGFHMHVRSGLQTPYLSDEFMGFIKFCQQKAEEEGMLAYLYDEDRWPSGY
                     AGGFVTKNPKYREKYLRLTTKKLTSFPKEEAIEKGLTYLFACYDIVLNDEGKLVSARL
                     IDENDEAEGIKRYAYVETSPVSERFNFFTKVDSLCKEAIDEFIKVTYEAYKKHVGDKF
                     GTTHPAIFSDEPQYRRFVALPSPFSDEDAICAFTTDLPESFEKATGCSLKEGLPEIFW
                     NPAGQDYSKVRYLFFDHLCERFNNAFADNIYKWCDENGICFTGHMVEEPTLESQTKSI
                     GEAMRAYRSFHIPGIDILCEHMEYNTAKQCQSAKNQYGREAMMSELYGVTGWDYDFRG
                     HKFQGDWQAALGVTLRVPHLTWMSMKGSAKRDYPACIGYQSPWYKEYPYIEDHFARLN
                     TAMTRGMPVVKVGVIHPIESYWLIYADNLTSMGLKEDLDDSFAALTEWLLFTQNDFDF
                     ICESTLPEIYKDSEKGFTVGEMTYDAVIVPPMKTIRSTTLDRLESFSRKGGKLIFTGE
                     VPMLVDGEKSDRALKLSEKALKIPFSKNAIAKAIADEKIVFVINKSGDSTGNFVYQLR
                     DDGDSLWLLLAQGMKANNPDVIKPFDAIISIKGEFTPQLYDTLSGEITDLDCAYKGGK
                     TVIERVIHGHDSLLIRLIKGKKAQKAKAPQFEERVINIIDFKETVSYKRAEPNVYVLD
                     IAEWWLNDGEWQPKEEMLRIDKKCREILSFPGADGGGAQPWVLPDEPPKNFVTFRYHI
                     PSVHFVAAPLLAVEEIVSGKLNGEEFPIEFTGYFTDLDIKTIKLPPIQKGDNILEITM
                     PITQRISTEYAYLLGEFDVSVKGCTKTILPPSDKIGFSDITSQGLPFYGGNLSYFAEI
                     EVPDCDLIIRANQYRGSLLKVFFDGEEAGKIVFSPYKLRINGVKSGKHKIEILLFGNR
                     HNTFTSLHNANSSMTYVSPGNWYHTGENWCYEYRIKPMGLLTSPVIEVIQKV"
     gene            3220..3852
                     /locus_tag="PLD98_05390"
     CDS             3220..3852
                     /locus_tag="PLD98_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05718.1"
                     /translation="MQKEHIFRRAFMGGFNREDVLRYIEELKLNESLLMRKFKEKEEE
                     FSRTLLEKEVQFKDALAAKDRELSEANEKISRLQSDIAIALKECERLKAESDKKEAIA
                     QQLGNAIIDARKYSDQLIERSKQEAKRIDEATAKTVDTVSQKIDELELDINKTARQFA
                     TAFNALLDELEELKNSLSGFSTKEVEGQNRMESKTFETIKDNVYDLSNSL"
     gene            3865..4401
                     /locus_tag="PLD98_05395"
     CDS             3865..4401
                     /locus_tag="PLD98_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HQA05719.1"
                     /translation="MNVYHINTNELPQYADILKAGDRVKLSGTVYTARDAAHKRIMSL
                     MENGEALPFDLNGAVIYYAGPTPTRDDGQIGSFGPTTSSRMDKFAPMLLDNGLAAMIG
                     KGDRSKEVYEAIKRNKAIYFCAVGGAGALAAKSIKSTEEIAFFDLGCESVKKLDFNEF
                     PLIVGIDANGGSVFNTQD"
     gene            4495..5556
                     /locus_tag="PLD98_05400"
     CDS             4495..5556
                     /locus_tag="PLD98_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQA05720.1"
                     /translation="MPMLPFDIESILSGGVLSVISILLITAVIFVNGWTDAPNAIATC
                     ISTRSLTPAKGILLAAIMNFAGVFIMSMINRSVAKTVYGIASFGEDSKIALAALSASL
                     LSIVIWAVVAWFFGIPTSESHALIAGITGASFAVFGSFKGLGAAEWKKVLWGIILSVL
                     IGFGLGLLIVTLTRLIVQRFKLKGTTGVFRRLQVLSSAFMAFMHGAQDGQKFIGVFLL
                     SAYISNNRAKGGEFHIPIWLMLFTALVISLGTATGGYRIIKSVSMDMVKLKAYQGFSA
                     DLAGSVSLLLSSFAGLPVSTTQAKTSAIMGVGASRGLRFVNWRIAGNMVATWLLTFPG
                     CAFISYFVTKLFFVLIGKS"
     gene            5582..6151
                     /locus_tag="PLD98_05405"
     CDS             5582..6151
                     /locus_tag="PLD98_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05721.1"
                     /translation="MKNRIDYIDSLTDIIKILKQEAAEIIKYLEMSPEQRFYSSAKAP
                     DCTNNVSDITRQIVMRLSKELILEIEKDDIAFLALELNSISALLTVFRKHLLGSRPNI
                     KTTALFNELISALNELENTFLMLPNIKKSGIKIIEKCFALTENLNQKQNELLNYGVMP
                     DSAQLSLMKIITSFKNITERIIYAVMRVA"
     gene            6171..8618
                     /locus_tag="PLD98_05410"
     CDS             6171..8618
                     /locus_tag="PLD98_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQA05722.1"
                     /translation="MNSEIFLNLRREIIKKEFSRMNDRQFEAVTETEGPLLILAGAGS
                     GKTTVLVNRIANVIKYGRAYKSDYLCRNVTDEDIGAAKRLLAGDKSAYEEISDLLAVN
                     PARPWEILAITFTNKAANELKERLANMLGQDAGEIWASTFHSACGRILRRDGERIDYS
                     SHFTIYDTDDSKRLIKEVQSYLGIDDKFISHKSILSAISRAKDSLIGPNDFIMSAGSD
                     LRLQKIGQAYAEYQKRLKKADAMDFDDMVVNTVKLFKEHPDVLEKYSRKFRYIFVDEY
                     QDTNHAQYLLVSLLSKAWNNICVVGDDDQSIYKFRGATIENILNFEKHYKDAKIIRLE
                     QNYRSTRNILNAANSIIVNNIGRKGKSLWTQNAEGEKVTVVVCADETDEGTFVANEII
                     DSSAAGRKWNDHAVLYRMNAQSNSFERAFTRAGIPYRIVGGLRFYERKEIKDVIAYLA
                     VIVNPSDEIRLRRIINEPKRGIGEASLNKAAEIASALGLDLFSVISNAADYPALSKSA
                     GKFQKFAELINELRDQSEKMPPHELFSLMLEKTGYLKSLENDPETFEERFENIQELYS
                     NIVRFQEENPGAGIPEFLEDVALLSDIDNYNSEADAVVMMTLHSSKGLEFPLVFIAGM
                     EEGIFPGRQSLYDPNEIEEERRLAYVGVTRAKEKLYLTRAKTRMLFGNTTHNQPSPFL
                     LEIDPAYVNEIKTQRASGSSSLFGSSYGSGYSYQSRSSYYSSSDTSQGSSSSSQTSPA
                     PQRAGRTFTVGTVSSAQNKEPLDSYRAGDSVKHKKFGVGVVVSAEPVGNDILLEVAFM
                     ESGTKKLMARAARLEKL"
     gene            complement(8620..9801)
                     /locus_tag="PLD98_05415"
     CDS             complement(8620..9801)
                     /locus_tag="PLD98_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQA05723.1"
                     /translation="MYKIINAIKDFIQATDKILISLSILTSVYGLLMVYSATKSSLSE
                     GAIISRDFRTMLIAVGIGIVFAIIISLIDYELIIKLWPLIGFFCIGIMIVTLLWGKAP
                     PARPDARSWLVIGDTGFYLQPSELVKVGFIITFGIHLDSVKDTLDKIKTLFLLCVHGA
                     IPVFLVIITGDAGSALIFIIIFIGMMYMAGVKLRYFAAGAGLVAAISPLAWIYALKDL
                     QKQRILALFYPDLYPAAIYQQDMGIRAISNGGFWGSGLFKGTFTQGSLVPENHNDMIF
                     TVIGEELGFVGAVTAIVLIALIIARIIGTGKKSRDNSTANMCYGVAIMLAAQAVVNIG
                     MCLKLFPVVGITLPFFSAGGSSNLSVYIALGLILSIYRYNQTKQLDNLMLIGIRTPFT
                     E"
     gene            9905..10342
                     /gene="tsaE"
                     /locus_tag="PLD98_05420"
     CDS             9905..10342
                     /gene="tsaE"
                     /locus_tag="PLD98_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQA05724.1"
                     /translation="MREYFTNSERETEELAARLAGEFKKGGVLAFKGGMGMGKTAFIR
                     GLVKGLGNTAFVSSPTFAIVNDYGGEPHTFHFDMYRVSSWEDLYSTGFFDYLAEGNLL
                     LIEWSENIEEALPEDTIFIEIEKGEGDTQRIIRISGKEEGNIV"
BASE COUNT         2936 a         2360 c         2466 g         2753 t
ORIGIN      
        1 aactttctgg ataacttcaa taaccgggga ggtcaaaagt cccataggct tgattctgta
       61 ttcgtagcac cagttttcgc ctgtgtgata ccagttgccg ggtgaaacat aggtcataga
      121 ggagtttgcg ttgtgcagag aagtaaaggt gttgtgcctg ttgccaaaga ggagaatttc
      181 tattttatgc tttccacttt taacaccgtt aattctgagt ttatagggcg aaaatacgat
      241 tttgcccgcc tcctcgccgt cgaaaaatac tttcaaaagc gagcccctgt actggttagc
      301 ccttataatc aaatcgcagt ctggaacctc aatttccgca aagtagctga ggttgccgcc
      361 gtagaagggc agaccctgag atgtaatgtc cgaaaagcct attttatcgg agggcggcag
      421 aattgttttt gtgcagccct taacggacac atcaaattca cccaagagat aagcgtattc
      481 ggtcgatatg cgctgcgtaa tgggcattgt aatttcaaga atgttgtcgc ccttttgaat
      541 cggcggcagc tttattgttt taatgtctaa atcggtaaag tatccagtga actcaatggg
      601 gaattcctcg ccgtttagct ttccgctaac aatttcctca acggcaagca ggggagcggc
      661 aacaaaatgt actgagggta tatggtatct gaaggttaca aagtttttag gcggctcatc
      721 gggcagaacc cacggctgag ctccgccgcc gtccgcgccg gggaagctga gaatttccct
      781 gcatttctta tcaattctca gcatttcctc tttgggctgc cattcgccgt cgttcagcca
      841 ccactcggct atatccagaa catagacatt cggctcagcc ctcttatagc tgacggtttc
      901 tttaaagtct ataatgttta ttactctttc ttcaaattga ggagcctttg ctttttgagc
      961 tttcttgccc tttatgagcc taataagaag gctgtcatga ccgtgaatta ccctttcaat
     1021 tacggtcttt ccgcctttat aagcgcagtc taaatccgta atttcgccgc tcaaagtgtc
     1081 gtaaagctga ggagtaaatt cgccttttat gctgattata gcgtcaaagg gttttataac
     1141 atcggggtta ttagccttca tgccctgagc caaaagcagc cacaaagaat ccccgtcatc
     1201 cctgagctga taaacaaaat tgcctgtgct gtcgccgctt ttgtttatga cgaatactat
     1261 cttttcatca gcgatagctt ttgcgattgc gttcttgctg aaggggattt ttaatgcctt
     1321 ttcggaaagt ttcagcgccc tgtcggattt ctcgccgtca acaagcatcg gcacttcgcc
     1381 tgtgaaaatc agctttccgc ctttccggga gaaactctca aggcggtcaa gggtggtgga
     1441 gcgtatggtt ttcatcggcg gaactattac cgcatcatag gtcatttcgc ccacggtaaa
     1501 gcccttttcg ctgtctttgt aaatttcggg cagggtggac tcacagataa agtcaaagtc
     1561 gttttgagta aagagaagcc actcggttaa agccgcaaaa ctatcgtcca aatcctcttt
     1621 aagacccatg ctggtgaggt tgtcggcgta aataagccag taagactcaa tcgggtgaat
     1681 tactcccacc ttaacaacag gcattcctcg tgtcatagcg gtgtttagcc tagcaaaatg
     1741 gtcttctata tagggatatt ccttgtacca gggggattga tagcctatgc atgcgggata
     1801 atccctcttt gccgagccct tcatggacat ccaagtaaga tggggaactc ttaaagtaac
     1861 gccgagagcc gcctgccaat cgccctggaa tttgtgccct ctgaaatcat aatcccagcc
     1921 tgtgacgccg taaagctccg acatcatagc ctctctgccg tactgattct tcgccgactg
     1981 acactgcttt gcggtgttgt attccatgtg ctcgcacaga atgtcaatgc cgggaatatg
     2041 aaagctcctg taagccctca ttgcctcgcc gatactcttt gtctggcttt cgagggtagg
     2101 ttcttcaacc atatgacccg taaagcatat gccgttttca tcgcaccatt tgtatatgtt
     2161 atcggcaaag gcattgttga acctctcgca aaggtggtca aagaacagat agcgcacctt
     2221 tgaatagtcc tgaccagcgg gattccagaa aatctcgggc agaccttctt ttaagctgca
     2281 gcccgttgcc ttttcaaagc tctcgggtaa atccgtggta aatgcgcaaa tcgcgtcctc
     2341 gtccgaaaaa ggcgagggga gggctacaaa ccttctgtat tgcggctcat cgctaaaaat
     2401 cgcgggatgt gtagtgccga atttgtcccc cacatgcttt ttgtaagcct cataagtaac
     2461 ttttataaat tcatcgattg cctctttgca gagtgagtct acctttgtga agaaattaaa
     2521 tctctcgctt acgggtgatg tctcaacata agcgtatctc tttatgcctt cagcctcatc
     2581 gttttcgtca atgagccttg ctgatacaag cttaccttca tcgttcagca ctatatcata
     2641 gcatgcaaaa agatatgtaa gacccttttc aatagcttct tctttcggga aagaggtgag
     2701 cttttttgtt gtgagtcgca ggtatttctc cctgtactta ggattcttag taacaaagcc
     2761 gcccgcatag ccggagggcc atctgtcctc atcataaagg taggcgagca ttccctcttc
     2821 ttcagccttt tgctggcaga actttataaa gcccatgaat tcgtcggaaa gatagggagt
     2881 ctgaagaccc gagcggacat gcatatgaaa gccgccaaag cccatatcct ttaaatcctt
     2941 aatttccttc tccaaaagct catttgtgag cttgcagttc catgaccaga agggagcgcc
     3001 tctgtattcg cttgaaggct taatgaataa gtcctctttt aactcttttt ctcttgactt
     3061 cgggtacagc attcgacatc acctcttttg tattatataa caaaggaaag ttattacaat
     3121 aacaaataat aaattgagga caatctaaat aaatttggtt gtttttggcc aaagctcctg
     3181 ttataatgtt ttcgaaagac gagtttaagg agggtaaata tgcaaaagga gcatattttc
     3241 cgcagagcct tcatgggcgg ctttaacaga gaagatgttc tgcgatatat agaagaacta
     3301 aagctcaatg agtctttgct catgagaaaa tttaaggaaa aggaagagga gttcagcaga
     3361 actcttcttg aaaaagaggt tcagttcaag gacgctttgg ccgcaaagga cagggaactg
     3421 agtgaggcta atgagaaaat cagccgcctt cagtccgata ttgccatagc cctaaaagaa
     3481 tgcgagaggc tcaaggctga aagcgataaa aaagaggcta tcgctcagca gcttggaaac
     3541 gcgataatcg acgcaaggaa atactcagac cagcttatag agcgctccaa gcaggaggca
     3601 aagcgcattg acgaggcaac agccaagaca gttgacaccg tcagccagaa aattgatgag
     3661 ctggagcttg acatcaacaa aaccgcaagg cagtttgcca cagcatttaa cgctttgctt
     3721 gatgagcttg aggagcttaa aaactcattg agcggctttt ccacaaaaga ggtagagggt
     3781 cagaaccgaa tggagtccaa gacctttgag acaatcaaag acaatgtcta cgacctttca
     3841 aattcacttt aaggacctat ggttatgaat gtttaccata taaacaccaa tgagctgccg
     3901 cagtatgcgg atattctcaa ggcgggcgac agagttaagc ttagcggcac cgtttacacc
     3961 gctcgtgacg ccgcccataa aagaataatg agccttatgg aaaacgggga ggcactgccc
     4021 tttgacttaa atggcgcagt tatttactac gcaggcccca cccctacaag ggacgacggt
     4081 caaataggca gcttcggccc caccacctca tcaagaatgg acaagtttgc ccccatgctt
     4141 cttgacaacg gtcttgccgc catgataggc aagggcgaca gaagcaagga ggtttatgag
     4201 gcaattaaaa gaaataaggc gatatacttc tgcgccgtcg gcggagcggg agcattggcc
     4261 gcaaaaagca taaagtctac cgaggagatc gccttttttg atttgggatg tgagtccgta
     4321 aagaaactcg actttaatga gtttccgctg attgtgggta ttgacgcaaa cggcggctcg
     4381 gtttttaata ctcaggatta gacaagaaat ttcggcactt tttctaaata aagttttaaa
     4441 ctttaggaag caacatactc atagaatagt acgcacaagc tgctgttcgg agtgatgcca
     4501 atgcttcctt ttgatattga atccatactg tcgggcggcg ttttgtcggt tatatctatt
     4561 cttcttatta cagctgtcat atttgtaaac ggctggacgg acgcgccgaa tgcaattgcc
     4621 acctgcatat caaccaggtc gcttacgccc gcaaagggaa ttttgcttgc cgcaataatg
     4681 aattttgcgg gtgtttttat aatgtctatg ataaaccgct ccgttgcgaa aacggtttac
     4741 gggatagcaa gcttcggcga ggactcaaaa atcgcccttg cggccctctc agcatcactt
     4801 ttgtcaattg tgatttgggc ggttgtcgct tggtttttcg gcatacccac aagtgaaagc
     4861 catgcgctga ttgcgggcat cactggagcc tcattcgcgg ttttcggaag cttcaagggt
     4921 cttggcgccg cagagtggaa aaaggtgctg tggggcatta tcctttccgt tttgataggc
     4981 tttggtttag gtcttttaat cgttactctc acccgcttaa ttgttcagcg ttttaagcta
     5041 aagggcacta cgggagtatt tcggcgcctg caggttctgt cgtccgcgtt tatggcattt
     5101 atgcacgggg cgcaggacgg gcagaagttc atcggagttt ttttgctcag cgcctatatc
     5161 tcaaataaca gggcaaaagg cggcgaattt catatcccta tttggcttat gctttttaca
     5221 gcgcttgtca tcagccttgg caccgcgacg ggcggataca gaattattaa atccgtcagt
     5281 atggatatgg tgaaactcaa ggcatatcag ggtttttccg ccgaccttgc gggctccgtt
     5341 tctctgcttt tgtcttcctt tgcggggctg cctgtaagca cgactcaggc taaaacctct
     5401 gctattatgg gcgtgggggc gtcaaggggg ctaagatttg tcaactggcg gattgcgggc
     5461 aatatggttg ccacatggct tttgaccttt cccggctgtg cgtttatctc atatttcgtc
     5521 accaagctgt tttttgtttt gattggcaag tcttgaatct aaaggaaacg gcggtgatga
     5581 aatgaagaat agaattgatt acatagatag ccttacagat attatcaaga tattaaagca
     5641 ggaagcagcc gagattataa aataccttga aatgagcccc gagcagcgtt tttacagctc
     5701 tgccaaagct cccgattgta ctaataatgt gagcgacatt acaaggcaga ttgtaatgcg
     5761 gctttcaaaa gagctgattc ttgaaatcga gaaggacgat atcgcatttt tagcccttga
     5821 gctgaacagt atttccgctc ttttgaccgt gttcagaaag catctgcttg gctcaagacc
     5881 taatatcaag accactgctt tgtttaatga gctgatttca gctttgaatg agcttgaaaa
     5941 cactttctta atgctgccca atataaaaaa gagcggcatt aaaatcatag agaaatgctt
     6001 tgctttaacg gaaaatctaa atcaaaagca aaatgagctg ttgaactatg gagtcatgcc
     6061 tgattccgcg cagctgagct taatgaaaat aatcacatcg tttaaaaata taacggaacg
     6121 aataatctat gcggttatgc gtgtcgctta gccgcaaaaa ggggaaaaca atgaattcgg
     6181 aaatcttttt aaatttaagg cgagaaatca taaaaaaaga attctcaaga atgaacgaca
     6241 ggcagtttga agccgttacc gaaacggaag gaccgctgct catccttgct ggcgcgggca
     6301 gcggcaaaac taccgtgctt gtaaacagaa tagcgaatgt tattaaatac ggcagagcat
     6361 ataagtccga ctacctttgt cgaaatgtca ccgatgaaga cattggcgcg gcaaagagat
     6421 tattggcggg tgacaagtca gcgtatgagg aaatatctga cctccttgcg gtaaatcccg
     6481 caagaccatg ggagattctt gccataacat ttaccaataa agccgccaat gagctaaaag
     6541 aaaggctcgc caatatgctg gggcaggacg cgggtgaaat ctgggcaagc accttccatt
     6601 ccgcatgcgg cagaattctc agaagagacg gcgaaagaat agactattcc agccacttta
     6661 caatatacga taccgacgac tcaaagcgtc ttattaaaga ggttcaaagc tatttgggaa
     6721 ttgacgataa gttcatatcc cataagtcaa tactttcagc tatcagccga gccaaggatt
     6781 cgcttatagg gcctaatgac ttcattatgt ccgcgggcag cgatttaagg cttcaaaaaa
     6841 taggacaagc ctatgccgaa tatcaaaagc ggcttaaaaa agccgacgcc atggactttg
     6901 acgacatggt tgtaaacacc gtaaagctct ttaaagaaca tcccgatgtc cttgaaaaat
     6961 attcaagaaa gttcagatat attttcgttg acgagtatca ggacacaaac catgctcagt
     7021 acctgcttgt aagccttctt tcaaaggcat ggaacaatat ctgcgttgtg ggcgacgacg
     7081 accagagtat ttataagttc agaggtgcca caatcgaaaa catactcaat ttcgaaaagc
     7141 actacaaaga cgcaaagatt ataaggcttg agcaaaacta ccgctccacc cggaacatac
     7201 tcaacgccgc taactccatt atcgtgaaca atatcggcag aaagggcaaa agcctttgga
     7261 ctcaaaacgc cgagggtgaa aaggtgacag tggtagtctg cgccgatgaa accgatgagg
     7321 gtacctttgt agcaaatgaa ataatagaca gctccgccgc gggcagaaag tggaacgacc
     7381 atgccgtttt atacagaatg aacgcccagt caaacagctt tgagcgggcg ttcacaaggg
     7441 cgggcatacc ttatagaatc gtcggaggtc ttaggttcta cgaaagaaaa gaaataaagg
     7501 atgtaatagc ctaccttgcg gtaattgtaa acccctccga tgaaataagg ctcagaagga
     7561 taataaacga gcccaaaagg ggtataggcg aggcaagtct taataaggct gcggaaattg
     7621 cctctgcctt gggactcgat ttgttttcgg ttatcagcaa tgcagcggac tatcctgcgc
     7681 tctcaaagtc tgctgggaaa ttccagaagt ttgcggaact tattaatgaa ctgagagacc
     7741 aatctgaaaa aatgccgccc catgagctgt ttagcctcat gctcgaaaag accgggtatc
     7801 tcaaatccct tgaaaacgac cccgaaacct ttgaggagag atttgagaac attcaggagc
     7861 tttattcaaa catagtaaga ttccaggaag aaaacccggg agcgggcatc cccgagttcc
     7921 ttgaggatgt cgcattgctt tccgatattg ataattacaa ctccgaagcc gacgccgttg
     7981 taatgatgac ccttcactca tcaaagggtc ttgagttccc ccttgtattc attgcgggta
     8041 tggaggaagg catattcccg ggcagacagt cgctttatga cccgaatgaa attgaggagg
     8101 aaagacgcct tgcctatgtg ggcgtcacaa gggctaaaga gaagctgtat cttacacggg
     8161 cgaaaaccag aatgcttttc ggcaacacta cccacaatca gccttcaccc ttcctgcttg
     8221 aaatcgaccc tgcctatgta aatgagatta aaacccaaag ggcgtctggc agctcctcgc
     8281 tgttcggctc gtcgtacggt tcgggctact cctatcagtc aaggagcagc tattactcat
     8341 cttcagacac atctcaaggc agctcatcct cttctcagac aagccccgcc ccgcagagag
     8401 cgggcagaac ctttacggtt ggcaccgttt caagcgcgca aaataaagag ccgctcgact
     8461 catacagagc cggtgactcg gttaagcata aaaagttcgg cgtaggcgtt gttgttagcg
     8521 ctgagcccgt gggcaacgat attctgcttg aggttgcctt tatggaatcg ggcacaaaga
     8581 agctgatggc aagggcggca aggctcgaaa agctgtaagc tattcggtaa acggggttct
     8641 gattcctatc agcatcaggt tatcgagctg cttagtctga ttatatctgt aaatgctgag
     8701 aatgagaccg agagctatgt aaacactaag gtttgaggag cctcccgcgc tgaaaaacgg
     8761 cagtgtaatg cccacaacgg ggaaaagttt aaggcacata ccaatgttaa caaccgcctg
     8821 cgcggcaagc ataatggcga ctccgtagca catgtttgcc gtcgaattgt cccttgattt
     8881 cttgcctgtg ccgattattc ttgctataat taatgcaatc aggacaattg cggttacggc
     8941 tcccacaaag cctagctctt caccgatgac cgtaaaaatc atgtcattat ggttttccgg
     9001 cacaagagag ccctgtgtaa atgttccctt gaacaagccg ctgccccaaa agccgccgtt
     9061 gctgatagct cttatgccca tatcctgctg ataaattgcg gcagggtaaa ggtcgggata
     9121 gaaaagggcg agaatccttt gcttttgaag gtctttaagg gcgtaaatcc atgcaagcgg
     9181 tgaaatcgcc gcgaccaaac ccgcgccagc cgcaaagtat ctgagtttta cgcctgccat
     9241 atacatcatg ccgatgaaga taataataaa gataagcgcg gagcctgcgt caccagtaat
     9301 aatgacaaga aaaacgggta tcgcgccgtg aacgcaaagg agaaataagg tctttatttt
     9361 gtccagtgta tccttcacgc tgtcaaggtg tattccaaaa gttatgataa agccgacctt
     9421 gacaagctcc gaaggctgca gataaaagcc cgtatcgcct ataaccagcc atgaacgggc
     9481 gtcgggtctt gcgggcggcg ccttgcccca aagcagtgtc acaatcataa ttccgatgca
     9541 gaaaaatcct ataagcggcc acagctttat tatcagctca tagtcaatga gcgagattat
     9601 tatggcaaaa acaattccaa tccccacagc gatgagcatg gttctgaaat ctcttgaaat
     9661 aatagcccct tcgcttaacg aggatttagt tgcgctgtag accatcaaaa gcccgtacac
     9721 cgaggttaaa atgctgaggc ttattaggat tttgtctgtt gcttgtataa aatcttttat
     9781 ggcgttaatt atcttataca tctgataccc cttttcgggt tttatgtttt tcgactaaag
     9841 actgcaaaaa gattataatg ctttatgtcc tcattatcaa gtggagagta aatttagtga
     9901 gattatgaga gagtatttta caaacagcga aagagaaacc gaggagcttg ccgcaagact
     9961 tgcgggcgag tttaaaaagg gcggcgttct tgcctttaaa ggcggcatgg gaatgggcaa
    10021 aaccgccttt atccgcggac ttgtaaaagg tctcggaaac accgcttttg taagcagccc
    10081 cacctttgcc atagtcaatg actacggcgg cgagcctcat actttccatt ttgatatgta
    10141 tagggtaagc tcatgggagg atttgtattc cacaggcttt tttgattatc ttgctgaagg
    10201 aaatctactt ttaattgaat ggagcgaaaa tatagaagaa gccctgcccg aagatacgat
    10261 atttatcgag attgaaaagg gcgagggcga tactcaaaga atcatcagaa tttcaggcaa
    10321 ggaagaaggc aatatcgttt gaaggtttta ggaatagaaa catcagccgc agcggcaagc
    10381 gcggctgtcg cggaaaactc aaaggttatt agcgaagcct atataaatat cggtcttacc
    10441 cactctcaga ctctcatgga gctgattgac gattgtctca gaagggcaaa caccagcatt
    10501 tccgaaattg acgct
//
LOCUS       DAONVQ010000066        10486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4879_length_10486_cov_4.641933, whole genome shotgun sequence.
ACCESSION   DAONVQ010000066 DAONVQ010000000
VERSION     DAONVQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4879_length_10486_cov_4.641933"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="PLD98_05425"
     CDS             complement(<1..96)
                     /locus_tag="PLD98_05425"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HQA05725.1"
                     /translation="MFAGGCFWCMVQPFDSLLGIKKVVSGYTGGHT"
     gene            57..4760
                     /locus_tag="PLD98_05430"
     CDS             57..4760
                     /locus_tag="PLD98_05430"
                     /inference="COORDINATES: protein motif:HMM:NF037085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HQA05726.1"
                     /translation="MVEPCTKNIHPQKLLFLLYSLFNISIFAGTENITSYYNVDGSTP
                     NKIAVTYEFKIHGTSTWSTGFPTDAGTYDIRVTLASDTVIRNGGRNVNATSAQGTVTI
                     QKATPNPGNPTVTLTYGQSLSQATIQNAPGVEGTWVFDTPDVIPNVSNSNTTLYGATF
                     VPASSNYVSIHRTFTVTVNKKTVEVTARPITQNYGTNPRTYEIVYDGFVLGENENTPG
                     VFSTASRPTASCAYYQYADVGQYPITISLAGVSSANYNFVLGPTAYVTVTPAPLTVTA
                     TAVNREYDGTVNVTVNFGSLVGILNNDNVYLSSNTTVGTVADKNVGSGKEVTFTIPAL
                     AGAKAGNYSVSVSNLPVRVNITKATPTPPAVSFPSMVYDPTRQLSSLELPQGVTWDNP
                     STVPTVNVTSYPATYTPADAVNYNTVSFNIPLTITKAPVVIKADNKSTTYGQAEPAFT
                     YTFIGYLGPDAQNPPPRTGNITASTDYNRDDPAKRGVGTYTITLSGNPQSDNYYYTFE
                     NGTLTVNKATVNVTVEDKTVVYGSAAPQFTFVYNLSDFFYNDTPANSVDASGAVFSCD
                     YTVGSPVANYQINLSGLSSANYTFNYIPGTLTVTKATLKVRANEITIPYGSPVPAFTV
                     SYIGWVGGDSDSNNDLTGSPAFTCDYVVGSPVTPEGYIIQVARGTLNSNNYNFEFERG
                     VLRVVKADPVITQWPTATVTYLDNLSDPEWGEAVTSVPGTFIFDDMTTVPTFEEGRTY
                     PATFRPQDSVNYNEVKGEILVIVNRRSISGSVTISGVAMVSSSLTADVTGITPVEAQQ
                     YLNYSWVVNGTEISTSNTTGELIEDYIDHVAVLTVTAYGPFKGTITINSPVITKFKLT
                     PTLADLIFAPPVNIDYDGQNHPLDVRPRNDVGQFTVLYNGSPTPPRRAGQYVITVDFA
                     GNEDYAATVGVYLGTFEIRKVDLYATYTVNTKVYDRTATASISNLKVTPLGNDVVTLN
                     TTGAVATFNNANAGENKPVTLTGVLLTGPDAGNYNLKIQNTTGTILPREVSITATPVS
                     RVYNGTTEITLQFTNGLIGVIPPDDVTYSQYYATAYVETPDVGTDKPLVDPEYWTLPP
                     LIGADASNYKLKVVVERPMVTITKANPTYVLPELPVIVYDPETTLADIPLTGGWSWVN
                     PNTVPTADRTSYLATFTPSDTHNYNSLNVQVPLTTQKATVYVRPNNVENLVYGSPVPS
                     FTLSFSGFKGNDNALNSITGGNVNFNTNYTQYSDVGEYLISLTGGYTSTNYQFVLQTG
                     TITVVKKDVTAVATAVSRPYAPANTNVTVNFGSISGIVNNDDVSLSYTSTTGTIDSPN
                     AGLRNVTFTPPTLVGSKAKNYNLIINTNIVVEIVKANPTGVVFPSQATVEYGLPLSAA
                     QFKNDFEIPVAVPGHFVFEDYTIVPDEIGSGRSYMVLFVPDDSGNYNSISQLVPLVVK
                     IRILNPEVSIVGKFEQEQIIYASVKGIPDDAKRYIKYTWFRGDPDGPNGTFTVVGTDA
                     TYQLTQQDVGQQIYLRVSIGDNVIQPYFAPDQTVVSETIKEITLSFWEKLARWFYNII
                     ASFQKLFDIAGRSLG"
     gene            complement(4925..5011)
                     /locus_tag="PLD98_05435"
     tRNA            complement(4925..5011)
                     /locus_tag="PLD98_05435"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4975..4977),aa:Leu,seq:caa)
     gene            5554..7914
                     /locus_tag="PLD98_05440"
     CDS             5554..7914
                     /locus_tag="PLD98_05440"
                     /inference="COORDINATES: protein motif:HMM:NF039380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQA05727.1"
                     /translation="MKNKSRGKLKLSEETLSVIKTVLKQSLMAAGAFTLAQASIFGRL
                     SPFAVALVSGVHISYLPAALLGSAAGYVMSGEISAAIRYIAALVIAALLYNTFIKIWG
                     YRKSRLFSVIASFAAIFSTGLVLSFAETLTASLLLLFLSEAIVAGGSSYFIKRSLSIR
                     LNRKALSSMSSQDMISLLFSFGILLLSLQWLTVGGISLARMAASFAIIAAARYGREAA
                     GSITGISAGATLSIADGMSYLAGGYSLGGLLAGVFGIFGRVPAALSFVAANIIAALVS
                     GSGELALTAFIETGFAAIVFCLLPSSAAESIELLFSQKSAVPVTDGIKNVLNSKINMV
                     ANVVAELSTNIKAVGNVAAKMAMPDINKVYIRVQDKVCRGCERKSYCWESGYNDTMNA
                     FNDMTLILKKGDSVSRDNMPRHFAESCLKRDMIAKSFNATFYEYAARSGAESRVLAVR
                     DAAAQQYSVLSMVLNDISRELSSDFSLDNESAEIVRGALENFGITVTNILSIIDKSGH
                     LQISFNSLPPKAPINKREMAEYLGEELGIPFELPITEVLPSGEVRVRLYERPPYKALL
                     SASQYTSSGNTYSGDAYQSFYDGTGNFIVVLSDGMGTGKRAALDGNMSAGLAAKLMAA
                     GFEADCTLKIVNAAMLLKSRDESISTLDIAKINLYTGETTIFKAGAAPSYARRKGKTV
                     KLEKSSLPLGIIQDMEFEKLSIKLYEGDILTLMSDGAADCPQEIIKEELMKAVGAGIA
                     DFSESLAKRAYKCAENKHKDDITVIGIALVRNKGFETALDNNSVAV"
     gene            complement(8006..9439)
                     /locus_tag="PLD98_05445"
     CDS             complement(8006..9439)
                     /locus_tag="PLD98_05445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HQA05728.1"
                     /translation="MKKVLVLIAVLLIAAYIIPASGNIPNSEGVFDINFGRISAEAAP
                     VYDPIPADTSMKVNVNAKAAVLMDAGTGKILMAMNPDQQLYPASVTKIMSMLLVCEAL
                     SEGKISLSDKVTCSEEAAKKGGSQIWLEPGEVMTVEELFKAMAIYSANDACTALAELI
                     AGSEAAFVNLMNQKAGMLGMTRTHFENCTGLDDTTTSHQTSARDVAIMSRELLKYEDI
                     FIKYTTTWMDSLRGGKTELVNTNRLVRTYKGITGLKTGTTAKAGHCVSATAKRDGLHL
                     IAVVMGSNNSNDRFNGARAMLDWGFANYESVIPEVDKTQITKVKVLHGVEQELYPHIP
                     EISPVLVAKGKSKDITQKVTLATDIEAPVEKGQVVGTIEFFLGKEKLGEYKLTAPEDI
                     KKMNLWDAILKLFGCFKKTEKDVKIEDRAKEKVTESVTEKATEAETEKKKEEKSKKKE
                     SAETTEKETKKAQESAEKKKDKTEDTN"
     gene            9918..>10486
                     /locus_tag="PLD98_05450"
     CDS             9918..>10486
                     /locus_tag="PLD98_05450"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQA05729.1"
                     /translation="MERYVIINADDFGMCRSANLAVFDLFKKGGITSATIMTPCSWAP
                     EAAAFARDNPEFAIGIHLTFTSEWSKYRWAPVNSENTASLRDADGYMYSDSKDFEKNC
                     VLEEVEAEARAQIERIKKLGLTNPSHLDNHMGSIYGIETGRLELLTLAFDLASEYNLP
                     FRFPATFTDEQFENTMLGINIPKELVLNM"
BASE COUNT         2755 a         2863 c         2379 g         2489 t
ORIGIN      
        1 agtgtgacca cctgtataac cagaaactac ctttttaatg cctagcaacg agtcaaatgg
       61 ttgaaccatg caccaaaaac atccacccgc aaaaattgct gtttcttctt tactcattat
      121 tcaacatctc catttttgcg ggcaccgaaa acattacctc ctactacaat gttgacggca
      181 gcaccccgaa caaaatcgca gtaacctacg aatttaagat tcatggaacc tcaacatggt
      241 ccacaggatt ccccaccgac gcgggaacct atgatataag agtcaccctg gcgtctgaca
      301 ccgtaataag aaacggcgga cgaaatgtca acgcaaccag cgctcagggc accgtcacaa
      361 tacaaaaggc aacccccaac ccgggcaacc cgacggtaac gctgacctac ggtcagagtc
      421 ttagccaggc aaccattcag aacgctcccg gcgttgaggg cacatgggta ttcgatactc
      481 ccgatgtaat tcccaatgta tccaacagca acactaccct ttacggagca accttcgttc
      541 ccgcaagctc caactatgtt tcgattcaca ggacattcac cgttaccgtt aacaaaaaga
      601 ccgttgaggt aacggcaaga cccattactc aaaactacgg aacgaatccc agaacatacg
      661 agatagtata tgacggcttc gttctcggcg agaatgaaaa cacccccggt gtattctcca
      721 cagcctcaag gcccaccgca agctgcgctt actatcagta tgccgatgtg ggacagtatc
      781 cgattacgat ttccttagcg ggagtcagca gcgccaacta taacttcgtt ttaggcccca
      841 ccgcttatgt aaccgtaact cccgcgcccc ttaccgtaac cgcgaccgcg gtcaacaggg
      901 agtatgacgg caccgttaat gtaaccgtca acttcggttc gcttgtcggc attctcaata
      961 acgacaatgt atatctctcc agcaatacaa cagtcggtac agttgccgat aagaatgttg
     1021 gctcaggcaa ggaagtcaca ttcaccattc ccgccctcgc gggcgcaaag gcaggcaact
     1081 attcagtatc ggtcagcaac ctgcccgtaa gagtcaacat cacaaaggca acccccactc
     1141 ctccggctgt atccttcccg tccatggtct acgaccccac aaggcagctc tcttcactgg
     1201 aactgcccca gggcgtaaca tgggataacc cctcaaccgt tccgacggtt aatgtaacaa
     1261 gctatcccgc aacctacaca cccgcggatg cggttaacta caataccgta agctttaata
     1321 taccgcttac aatcacaaag gcacccgttg tcatcaaggc ggataacaag agcacaacct
     1381 acggacaggc agagccggcg ttcacctata ccttcatagg ctacctcggt cccgacgctc
     1441 agaatcctcc gccgagaaca ggcaatatca cagcctccac agactataac agggatgacc
     1501 ccgcaaagag aggcgtaggc acctacacca tcaccctttc gggcaacccg cagagcgaca
     1561 actactacta cacctttgag aacggcacac tcaccgtaaa caaggcaacg gtaaatgtca
     1621 cagttgaaga caagaccgta gtttacggct ccgccgctcc tcagttcacc tttgtatata
     1681 acctctcaga tttcttctac aacgatacac ccgccaacag cgttgacgct tcgggagcgg
     1741 tattcagctg cgattacacc gtaggctcac ctgttgcgaa ctatcagata aacctcagcg
     1801 gactttcatc cgcgaactac accttcaact acatacccgg cacactcaca gtcacaaagg
     1861 caacccttaa ggtaagggct aatgagatta ccatacctta cggctcgccc gttcccgcct
     1921 tcactgtttc ttacatcggc tgggtaggcg gcgacagtga cagcaataac gaccttacag
     1981 gctcacccgc attcacctgc gactatgttg taggctcacc cgttacacct gaaggctata
     2041 tcattcaggt tgcaaggggc accttaaaca gcaacaacta caacttcgag tttgagcgcg
     2101 gcgttctcag agtcgtaaag gcagaccccg ttatcaccca gtggcccacc gccacggtaa
     2161 cataccttga caacctctcc gaccctgagt ggggcgaggc tgtaacctcc gtacccggca
     2221 ccttcatatt cgacgatatg accacagtgc ctacatttga ggagggcagg acctatcccg
     2281 caaccttccg cccgcaggat tcggtaaact acaatgaagt caagggcgag attcttgtaa
     2341 tagtcaacag gcgcagcatc agcggctccg taaccatcag cggcgtcgcg atggtatcaa
     2401 gctcgctgac cgccgatgta acaggcatta cccccgttga ggctcagcag tatctaaatt
     2461 actcctgggt agtaaacgga acggaaatct ccacaagcaa caccacgggc gagttaatag
     2521 aggactacat cgaccatgtg gcagtcctga cggtaaccgc atacggaccc ttcaagggca
     2581 ccattaccat aaacagcccg gttatcacca agttcaagct cacacccaca ctggctgacc
     2641 ttatcttcgc tccgcccgtc aatattgact acgacggtca gaatcatccg ctggatgtaa
     2701 gacccagaaa cgatgtgggt cagttcaccg ttctctacaa cggttcaccc acaccgccca
     2761 gaagggccgg ccagtatgtc atcaccgttg actttgcggg caacgaggac tatgccgcaa
     2821 ccgtaggcgt atacctcggt acctttgaaa tcagaaaggt tgacctgtac gcaacctaca
     2881 ccgtaaacac caaggtttac gacaggaccg caaccgcttc catttccaac ctcaaggtca
     2941 ccccgctggg caacgatgtt gtgacactta acaccacagg cgcagtggca accttcaata
     3001 acgcgaacgc aggcgagaac aagcccgtaa cccttacagg cgttctcctt acaggtcccg
     3061 acgcaggcaa ctacaacctg aaaatacaga atacaacggg aaccattctg ccgcgtgagg
     3121 tttcaatcac cgcaacaccc gtttcaaggg tatacaacgg cacaacagag ataaccctcc
     3181 agttcaccaa cggacttata ggcgttatcc cgcccgatga tgtaacctac tcccagtact
     3241 atgcaaccgc ctatgtcgag acacccgatg taggcaccga caagcccctt gtcgaccccg
     3301 agtattggac tctgccgccg ctgatcggcg cggacgcaag caactacaag cttaaagtag
     3361 ttgttgaaag accgatggtt acaatcacaa aggcaaatcc cacatatgta ctgccggagc
     3421 ttccggtaat tgtttacgat cccgaaacaa cccttgccga cattccgctt acaggcggct
     3481 ggagctgggt aaatcccaac acggtaccga cggctgacag aacctcctac cttgcaacat
     3541 tcacacccag cgatacccac aactacaaca gcctcaatgt tcaggtaccg ctcaccactc
     3601 agaaggcaac ggtttatgtc agacccaaca atgtggagaa tctggtttac ggctcgcccg
     3661 ttcccagctt cacactcagc ttctcgggat tcaagggcaa cgacaacgct cttaactcaa
     3721 tcacaggcgg caatgtcaac ttcaacacaa actataccca gtactccgat gtgggcgagt
     3781 acctcatcag cctcacaggc ggctacacaa gcaccaacta tcagttcgtg ctgcagacgg
     3841 gtacaatcac ggttgttaag aaggatgtaa ccgcggtcgc aaccgcagtt tcaagaccct
     3901 atgcgcccgc caatacaaat gtaaccgtaa acttcggctc catctcaggc attgtaaaca
     3961 acgacgatgt atccctctcc tacacaagca caacgggcac aatcgactcg cccaatgcag
     4021 gtctcaggaa tgtaaccttc acaccgccca cactcgtagg cagcaaggct aagaactaca
     4081 acctgattat caacaccaac atcgttgttg aaatcgtaaa ggccaacccg acaggcgtag
     4141 tattcccgtc acaggcaacc gttgagtacg gtctgccgct gtcagccgct cagttcaaga
     4201 acgactttga gattcccgta gctgttcccg gccacttcgt atttgaggac tacacaatag
     4261 ttcccgacga aatcggctca ggcagaagct acatggttct cttcgttccc gacgattctg
     4321 gcaactacaa ctccatctcc cagcttgtgc ctctggttgt aaagataaga atacttaatc
     4381 ctgaggtttc aatcgtcggc aagtttgagc aggagcagat aatctacgcc tccgtaaagg
     4441 gtattcccga tgacgcgaag cgctacatca agtacacatg gttcagaggc gacccggacg
     4501 gtccgaacgg aacatttacg gtcgtcggca ccgacgcaac ctaccagctc actcagcagg
     4561 atgtaggaca gcagatttac ctgagagttt caatcggcga caatgtaatt cagccctact
     4621 tcgcgcccga tcagaccgta gtgtccgaaa caatcaagga aataaccctt tcattctggg
     4681 agaagctggc aagatggttc tacaacatca tcgcctcgtt ccagaagctc ttcgacatcg
     4741 ctggcagatc cttaggctaa aattaaaaat cccccgacag tcaccctgtc ggggtttttt
     4801 tttgccgcat ttctcattta atgtttaccc caagaacaca gtcgggattg ctttatgcgc
     4861 agcatgatgc ctttatgctt tcccgcaaaa aaattgacct gtttggtttc caaacaggtc
     4921 tttttggtgc cggaagcggg acttgaaccc gcaccctgtt gccaggactg gattttgagt
     4981 ccagcacgtc tgccaattcc atcattccgg cgttttcgtc tgcaaaacat tgtacattaa
     5041 agccccaaaa aaatcaatag atatttaaag ggaaaatcat cttgctgaaa ccttatgcct
     5101 tcccctaaat cttcaataaa aaaacacaaa aagctgtaga gaaattggag aaatttacca
     5161 tatattgatt cgtttagcca atatttcggc agaattacca taattttaca gcgcacagtg
     5221 acaaaagctg tgagattaat caaatataat gtaataaaaa cataagagct tttgcgccct
     5281 tttttactcg tttaacggat attctttttg agaaattcgt ctaagcggct tagagttatc
     5341 ttttcagaaa acggcttttc ttaactgcaa taggcaaaac aattagggaa caaataacgc
     5401 aaatattagg acaagcccga tgcccggaaa tgtcccaaag agtcctcaat atttctttta
     5461 gaattagtat ttccaccttt tgaaccttcg cttagcattg ccttaaaggc tttaaataca
     5521 gtcaggtaat ataaaagcag agtggtgaaa tacttgaaaa ataaaagcag aggcaaactg
     5581 aagctttctg aagaaacgct gtcggtaata aaaacagtat taaagcagag cctaatggcg
     5641 gcgggcgcct ttactctggc gcaggcaagc atattcggca ggctgtcgcc ctttgcggtg
     5701 gcgctggtgt cgggcgtgca tatatcctac ctgcccgcag ctttgcttgg cagcgccgct
     5761 ggctatgtga tgtcgggcga aatctccgcc gcgataagat acatagccgc ccttgtcatc
     5821 gccgccttac tatacaatac atttataaag atttggggct accgaaaaag cagacttttc
     5881 tccgtcatag cgtcatttgc cgctattttt tcaacggggc tggtattgtc cttcgcggag
     5941 accctaaccg cctcgctgct gttgctattt ttgtccgagg cgatagtcgc cggcggcagc
     6001 tcttacttta taaagcgcag tctttcaata agacttaacc gcaaagccct gtcgagcatg
     6061 agctcgcagg atatgataag ccttcttttc tcattcggaa tcctccttct ttcccttcag
     6121 tggctgaccg tcgggggcat atcccttgcc agaatggcgg cgtccttcgc cattatcgcc
     6181 gccgcaagat acggcagaga ggcggcgggc tccattacgg gcatatcagc gggagccact
     6241 ctcagcatag ccgacggtat gagctatctt gccggaggct actccctcgg cggacttttg
     6301 gctggagtat tcggcatttt cggcagagtc cccgcggcat taagctttgt cgccgctaac
     6361 atcatagccg cccttgtttc cggcagcggt gagctggcac ttacagcatt tatagaaaca
     6421 ggctttgccg ccattgtttt ctgccttttg ccctcgagtg ccgctgagag cattgagctt
     6481 ttgttctccc aaaagagtgc cgttcccgta accgacggca tcaaaaatgt cctcaactca
     6541 aaaatcaaca tggtggcgaa tgtggtggcc gagctctcca ccaatattaa agcggtggga
     6601 aatgtggcgg caaaaatggc aatgcccgat ataaataagg tttatatcag ggttcaggac
     6661 aaggtctgcc gaggctgcga gcgaaaaagc tactgctggg agagcggcta caacgacacc
     6721 atgaacgcct ttaacgatat gacccttata ctcaaaaaag gggattcggt atcccgcgac
     6781 aatatgccga ggcactttgc cgaaagctgc ctcaaaagag atatgattgc aaagagtttc
     6841 aatgccacct tctacgaata cgccgcaagg tcgggagcgg aaagcagggt tctggcggtt
     6901 cgtgacgcgg cggctcagca gtattcggtc ctttcaatgg tgctgaacga tatttcaagg
     6961 gagctttcaa gcgatttttc ccttgacaac gagagcgcgg agatagtcag gggagccctt
     7021 gaaaacttcg gcataaccgt tacgaatata ctcagcatta tagataaatc ggggcatctg
     7081 caaatctcct ttaacagcct gcccccaaaa gcgcccataa acaagcgtga aatggcggag
     7141 tatcttggcg aggagctggg catacccttt gagctgccca taacagaggt tctgccgtcg
     7201 ggagaagtta gggtcaggct ctatgagcgc ccgccctaca aggcgctgct gtcggcgagc
     7261 cagtacacca gcagcggcaa tacttacagc ggcgacgctt atcagagttt ttacgacggc
     7321 acgggcaatt tcattgtcgt cctcagcgac ggcatgggca cgggcaagcg ggcggctctt
     7381 gacggcaata tgtccgcggg tcttgccgct aagctcatgg cggcaggctt tgaggcggac
     7441 tgcaccctca aaatcgtcaa cgcggctatg cttttaaagt caagggacga gtcaatatcc
     7501 acccttgata ttgcgaaaat aaacctctat actggcgaga ccacaatatt taaggcgggc
     7561 gcggcgccct cctacgcaag gcgcaagggc aagaccgtta agctggaaaa atcctccctg
     7621 cctcttggca taattcagga tatggaattc gaaaaactca gcataaaact ctatgaaggg
     7681 gacatactga cactgatgtc cgacggagcg gccgactgcc cgcaggagat aataaaggaa
     7741 gagcttatga aggctgtggg agcgggcatt gccgactttt cggagagcct tgcgaaaagg
     7801 gcgtataagt gcgccgaaaa taaacacaag gacgatatta ctgtgattgg aatagccctt
     7861 gtaaggaata agggctttga aacagcttta gacaataaca gcgtggcagt ttgatttctc
     7921 ccaaagtaca cagaagcacc ggcatttcgc cggtgcttct gcgtgtgggg ggaagtatat
     7981 atttaagatg gggaatttcg ttttttcagt tagtgtcctc tgtcttatct tttttctttt
     8041 cggcgctttc ctgagctttc tttgtctcct tttccgtcgt ttccgcagat tccttcttct
     8101 tggacttttc ctccttcttc ttttcggtct cggcttcggt agccttttcg gttaccgact
     8161 cagtgacctt ctcctttgct ctgtcctcaa tttttacatc cttctcggtc ttcttaaagc
     8221 agccgaagag tttcaggatt gcgtcccaaa ggttcatctt cttaatatcc tcgggagcgg
     8281 tgagtttgta ttcgcccagc ttttctttgc cgaggaagaa ctcaatggtt cccacaacct
     8341 gacccttttc aacgggagcc tcaatgtcgg tggcgagggt taccttctgg gtaatatcct
     8401 tgctctttcc tttagcgacg agtacggggc tgatttccgg aatgtgggga tagagctcct
     8461 gctcaacgcc atgcagaacc tttacctttg tgatttgggt tttgtcaacc tcgggaatta
     8521 ccgactcgta atttgcaaag ccccagtcaa gcattgccct tgctccgttg aaacggtcgt
     8581 tgctgttgtt gctgcccatg actacggcga taagatgcag accgtccctc tttgctgtgg
     8641 cggatacgca gtgacctgcc tttgcggtgg tgcctgtttt aagaccggta atgcctttat
     8701 atgtccttac aagcctgttg gtgttgacaa gctccgtttt gccgcccctt aatgaatcca
     8761 tccatgttgt ggtgtactta atgaaaatat cctcgtattt taaaagctcc cttgacataa
     8821 ttgccacatc ccttgctgag gtctgatgag aggttgtcgt atcgtcaaga ccggtgcagt
     8881 tttcaaaatg agtccttgtc atgccgagca ttccggcttt ttgattcatg agattgacaa
     8941 aagcggcctc gctgcctgct ataagctcag ccaaagcggt acaggcgtcg ttcgcgctgt
     9001 aaatggccat agccttaaat agctcctcta cggtcatgac ctcgcccggc tcaagccaaa
     9061 tctgagagcc gccctttttg gcagcctcct cgctgcaggt gaccttgtcg gagagggaga
     9121 ttttaccctc tgacaaagcc tcgcacacaa gcagcatgga cattattttt gtgaccgatg
     9181 cgggatagag ctgctggtcg ggattcatgg ccataaggat tttgcccgta cccgcgtcca
     9241 tcagcaccgc ggctttcgcg ttgacattta ccttcattga ggtgtccgcg ggaatcggat
     9301 cataaaccgg cgctgcctca gcgcttatcc ttccaaagtt tatgtcaaaa acgccctctg
     9361 agtttggtat gttccccgat gcggggatga tataggcagc aattagaagt accgcaatca
     9421 gaacaagtac ttttttcata aggaccctcc ctgattttgc atatgcggac ttgaccgaca
     9481 caaagcctca aaaaaagaca agtttcgcat attcttgtgc cgtcaagccg tccacggtca
     9541 ataataagta tgcgatgtcc atcaaaaaaa gacatacatt cttgcattta tacaaaattg
     9601 tccacgctcg gacaagatgt ccccgataaa gtcgttaagc cgcaaataaa agtttgtcca
     9661 acgggcggtt ttacaccccg cggagattgg cagaaaaaga cttcgaatct gccgacacac
     9721 tgccgattcc ggacagggga gcggaaaaca tccgccccgg aaacaagggc gtcaaaaatc
     9781 aaaattgtac atctcagatg caattaattg tggctttttt gtaaattttt acccgttgtc
     9841 aatggacagc gacaatgctt tgtgctacac ttatcggggt aagaaattag attttttata
     9901 tatttcggag gattattatg gaacgctatg taattattaa cgctgacgat ttcggtatgt
     9961 gccgttcggc aaaccttgcg gtttttgacc ttttcaaaaa gggcggcatc acatcggcaa
    10021 caataatgac cccctgctca tgggcaccag aggcagccgc attcgcgaga gataacccgg
    10081 agttcgctat tggtattcac cttacattca caagcgagtg gagcaagtat cgctgggctc
    10141 ccgtaaattc ggagaacacc gcatcccttc gtgacgctga cggctatatg tacagcgaca
    10201 gcaaggactt tgaaaagaac tgcgtccttg aagaggttga ggcagaggca agagctcaga
    10261 ttgaaagaat caaaaagctt ggtcttacca atccctctca ccttgacaac cacatgggtt
    10321 caatttacgg cattgagaca ggccgcttag agcttttaac ccttgcgttt gaccttgcca
    10381 gcgagtacaa tcttcccttc cgcttccccg caacctttac ggacgagcag tttgagaaca
    10441 caatgctcgg catcaacatc cccaaggagc ttgttctcaa tatgtt
//
LOCUS       DAONVQ010000067        10350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_4953_length_10350_cov_4.821078, whole genome shotgun sequence.
ACCESSION   DAONVQ010000067 DAONVQ010000000
VERSION     DAONVQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10350
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4953_length_10350_cov_4.821078"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>710
                     /locus_tag="PLD98_05455"
     CDS             <1..>710
                     /locus_tag="PLD98_05455"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HQA05730.1"
                     /translation="EYPAQINHREKGFAEVAFAAALPSVGLKAFDIRPSDEPCGIKSE
                     LKVGKDLLENEKYIVRIDEAGDIRSVYDKEIGRELLSSPVRMAELNYTGSLQYPAWEL
                     DYNDVTAPPREYAANPAIEIIENGPARVAIKIRRKSSKSIYTQIISLFKGSDRVEVFN
                     EVDWRSTRSILKAQFPLSAKNKKCRFDLGLGSIERGVSTPKLYEFPAQMWASQTSEDG
                     KYTVAVFSDSRTGWDKPD"
     assembly_gap    711..720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <721..1952
                     /locus_tag="PLD98_05460"
     CDS             <721..1952
                     /locus_tag="PLD98_05460"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HQA05731.1"
                     /translation="STIRLTAVHTPKARYREESAQHLMDLGLNRFGFALYSCEGDFTN
                     GVQQSATEFNQPPAAFVTTAHEGSLGGSFSFASISDAAVAVRAIKKAEESDEIIVRVN
                     ETSGRPKKNIVLAFASDILTAAETNGAEEYLRAAKTEGNKLIFDLGKFEVKTFSLKLK
                     QTGKETAPETEKVMLPLNIKVTSKNGEKLKGPLKGGSFIPREIYPESIPVKGLNFPID
                     KSGFALLCDGETLTLPENTEKLYILAAARDGDTECEFIAGNQSVKLTVADMFERVGKW
                     DLYALNETAHIKRDTLAWNFTHTHNEKGDEAARYAYLFMYELEIPPFVRTVKLPKGKK
                     IFVFSALAVKNDIPFTLANDLYDRAEKRDSFGFEVPAEVHEEAKLTAKEKKQARKKFV
                     RNYTKNRLQREISARKP"
     gene            1968..3119
                     /locus_tag="PLD98_05465"
     CDS             1968..3119
                     /locus_tag="PLD98_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05732.1"
                     /translation="MKFLKAAAAALPLLLLLSSCSNSHKAAKDIAVRAVMDVQSYENG
                     SIVEIPRLYYNSESRFLSEQGGEIPDIKLINSSIEKSFKANYEQFRKEKRKGCIDIRT
                     YPFDSERYVQLVITYNEYPTYGTDGFLQSFCFDKLKNELITAEERLRHDSITLPELKE
                     KAKELYTPEYEGDFLSDASLSGFVILEDRTLYLMTLTVRNENSPTRILFYSLSLSENS
                     LKRLDKDMLFDKGLLSRLDPPLYYDREEGANKRSFPLKLILKAENLNESGENKYSLEG
                     KTEITIKSSEPVPVFASHISELVKNAEGGKALDITCEYDLGLTEKIEYPAFVLEYKKP
                     LYGRELRCLDMVLVTESAAFRVHTETDEKDYYRIKDKIIEAFGSADFVY"
     gene            3383..3871
                     /locus_tag="PLD98_05470"
     CDS             3383..3871
                     /locus_tag="PLD98_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HQA05733.1"
                     /translation="MVCPKCNSEIMDEAVLCPECMCRVGFTNRPTYRLKTNRSLIKYI
                     LLSIVTFGIYSLIAMSGVSSDINIIAEKYDGKKTMHFLLVTFVLSWLTFGIYPIVWFH
                     KLSDRIGDELDRRGIGYDFGMKDYWLWAVLGSLIVIGPFIYLHKLLESMNLLSADYNE
                     RG"
     gene            complement(3909..5225)
                     /gene="murD"
                     /locus_tag="PLD98_05475"
     CDS             complement(3909..5225)
                     /gene="murD"
                     /locus_tag="PLD98_05475"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQA05734.1"
                     /translation="MSNTSRRLKEYFEDKNILILGFGKEGRSSYEFIRRVLPNKPLAI
                     ADMKDIELSDDNVRLITGEDYLSCLARFDLVLKSPGIPFKGVKIPDGTEITCQTDLFL
                     RFSDFTAVGITGTKGKTTTSTLIYSMLKEAGFDACLIGNIGVPVFEDIDISDSAVAVI
                     ELSSHQLQFTRTSPHIAVLTNIYEEHLDHYDGGFEGYVNAKLNIVRNQTEKDYFIYNP
                     DQNLEEYISLDSVKSKKIPCPSVSDEEFLNNLTKGNPHLKGRHNLHDAMMAAKAVRCL
                     GVGNDAIKRAVDNFAGIEHRMEYVGTFDDVEYYNDCIATIPKAVLSAVEALGNVGSLI
                     IGGLDRGLDYTDFADELIKSGIDNIICLPDTGYSIGKLMESKGATSRIIYAKDMEDAV
                     RHAKDFTPKGKAALLSPAAASYNLYKGFEEKGRHYKKLVLEICAKN"
     gene            complement(5200..6612)
                     /locus_tag="PLD98_05480"
     CDS             complement(5200..6612)
                     /locus_tag="PLD98_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05735.1"
                     /translation="MEAKDYNALREKYPVFKYNSFSLEKAEGGIRLSFDFEIEGLCRF
                     CPDTLIVTENLKILNDPFSPMARKIAFFIGMTELVSYYKAVLSPKVIISCGKMTKADE
                     EFFIKLYHNGLSEFFYTNNINIEKDKLLSFEYKGGEDFEGAESEEFKSSEINLIPVGG
                     GKDSAVTANLLRHHRDKNMFFTINDQKARTETVTAAGYGEDRIVRTYRKIDPELLELN
                     KKGFLNGHTPFSAIVAFLSYYCAYIIGAEHIVLSNEASANAPNIEGSEVNHQYSKSYE
                     FERDFFEFTKRNFTEKIKYFSMLRPFNELQIAKEFSDLPQFHSIFRSCNRGSKQNIWC
                     GECAKCLFVYSILSPFLPNEKLIEIFGSDLFEKEHLSEILDGLNGVIKLKPFECVGTV
                     EEVAAALNETVRQCESAGRALPPLLRRHKECFSGDSAPYKVPDIESILSHYNSEHNIP
                     EKFMNTVREMYSFVQHKQEA"
     gene            complement(6739..8019)
                     /locus_tag="PLD98_05485"
     CDS             complement(6739..8019)
                     /locus_tag="PLD98_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004065963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HQA05736.1"
                     /translation="MEVLRLGMRGPLVQMLQLALARAGYYSGEIDGIFGYQTQRAVFS
                     FQSAFGVTSDGVVGPATWGHILPYIRGYFYRVIRSGDTLWSLARQYGTTVRAILTANP
                     TLDPDSLRPGQTIVIPFGFPAVSTNIDYTSTLTTLIIDGLKARYPFLRTGNFGRSVMG
                     KSLHYISIGTGATQVFYNAAHHANEWITTPVLLKYVEDYARAYSENGSIFGTRAADLY
                     RGTTLYVAPLVNPDGVDLVTGAINRGTFYENAVRISSDYPNIPFPSGWKANIAGTDLN
                     LNYPAEWERAREQKFAAGFVSPAPRDYVGSAPLSAPESAAVYNFTLNHDFALILAYHT
                     QGEIIYWKFMDYDPPRAREIGERMSQASGYPLEITPEYSGYAGYKDWFIQEFNRPGYT
                     VEVGLGESPLPLSQFDKIYNDNIGILTIGMSSFI"
     gene            8426..9304
                     /locus_tag="PLD98_05490"
     CDS             8426..9304
                     /locus_tag="PLD98_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQA05737.1"
                     /translation="MLADLHCHTKLSDGSMGIEELILLAAGRGVSTIAITDHDCLAGN
                     VRGKVIGERHNVRVIPGVELSATDTLTGNEVHILGYLCDCPDRIEGLCHKNSMARRRA
                     GQYMMLKAAKKFPISPEFVLRCAQGSTNIFKQHIMHALMEIGYSSSMYGELYELLFSP
                     ESEENIIVNAKYSPVEEVIAAIHSAGGIAILANPAVYGGLELAERMLEHGLNGIEVWS
                     SDASEEDTKKFYEFAKKNKLLMTGGSNFHGMYNKGIWSVGDYGVHEDQVQELLQYKAR
                     LRRQQSKTETKQEVTV"
     gene            9439..10011
                     /locus_tag="PLD98_05495"
     CDS             9439..10011
                     /locus_tag="PLD98_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05738.1"
                     /translation="MRINDDSDIKIFGEKKKKDDFDPMLLLEEIKRHRSSGNTAKAKT
                     IAHHMADSIVESDIDESVKSFADRYEVELTSEVMHQVRVLSVFSAEYVLNKYLPSPLL
                     SSVAINELYESILAEAPEFYDRLESGTAFTFYYLAVRSGGNTPAEIGKAFASQCGRED
                     DGSCALFGTAVFTLNVETFQNLIFSFNFEE"
     gene            10102..>10350
                     /locus_tag="PLD98_05500"
     CDS             10102..>10350
                     /locus_tag="PLD98_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05739.1"
                     /translation="METHTPHRPRRGELNHPARRGEGASATTEPTPDRTQLSRRCTPR
                     RAAGVNPALLPAKRRGTPRERRRAGMPATPCAEGRTRQT"
BASE COUNT         2701 a         2606 c         2508 g         2525 t
ORIGIN      
        1 aagaataccc cgcccagata aaccatagag aaaagggctt tgccgaggtc gctttcgcgg
       61 cggctctccc gtcggtaggt ctcaaagcct ttgacataag accctccgac gaaccttgcg
      121 gcattaagtc agagcttaaa gtcggcaagg atttgctcga gaacgagaaa tacattgtcc
      181 gcattgacga ggcgggcgac atccgctccg tttatgacaa ggaaatcggc agggagctgc
      241 tctcaagccc cgtcagaatg gcggagctga attatacggg aagtcttcag taccccgcat
      301 gggagctgga ctataacgat gttacggctc ctcccaggga gtacgccgca aatcccgcaa
      361 ttgaaatcat agaaaacggc cccgcaaggg tggctattaa aatacgccgc aaaagctcaa
      421 agagcatata cacccagatt atttccctct ttaagggctc agacagggtg gaagtcttta
      481 acgaggttga ctggcgcagc acccgcagca tcttaaaggc tcagttcccc ctctcggcaa
      541 agaataaaaa atgccgcttt gacttggggc tgggcagcat tgagaggggc gtttccacgc
      601 ccaaactcta cgagttcccc gctcagatgt gggcatccca gacaagcgag gacggcaaat
      661 acaccgtggc tgtgttcagc gactcccgca ccggctggga caagccggac nnnnnnnnnn
      721 agagcacaat acgccttacc gccgtgcata cccccaaggc aagatacagg gaggaaagcg
      781 cccagcacct tatggatttg ggactaaacc gcttcggatt tgccctttac tcctgcgagg
      841 gtgatttcac aaacggcgtt cagcaaagcg caacagagtt caatcagccc cccgccgcct
      901 ttgtgacaac ggcgcatgaa ggatctttgg gcggcagctt ctccttcgcc tccatttccg
      961 acgccgctgt ggctgttagg gctattaaga aggctgagga aagcgacgag ataattgtca
     1021 gggtcaacga aacctcaggc agacccaaga agaatatagt tcttgccttc gcttccgata
     1081 tactcaccgc cgccgaaaca aacggcgctg aggaatatct cagagccgcc aaaacagagg
     1141 gcaataaact catattcgac ctcggcaaat ttgaggttaa gaccttctcg ctgaaactca
     1201 aacaaacggg caaagaaaca gcgcccgaaa cagagaaggt catgctaccg cttaacataa
     1261 aggtaacctc taaaaacggc gagaaactaa aaggcccctt aaagggcggc agttttatac
     1321 cgagagaaat ttaccccgaa tcaattcccg tcaagggtct taatttcccc attgacaaat
     1381 cgggctttgc ccttctttgc gacggcgaaa ccctgactct ccccgaaaac accgaaaaac
     1441 tctatatcct tgcggcggca agggacgggg acactgagtg cgagtttatt gcgggaaatc
     1501 agagcgtcaa gctcacagtc gccgatatgt tcgagagagt cggcaaatgg gacctttatg
     1561 ccctgaacga aaccgcccat attaagcggg acacccttgc gtggaacttt acacataccc
     1621 acaacgaaaa gggcgacgag gcggcaaggt acgcctacct ctttatgtac gagcttgaaa
     1681 tcccgccctt tgtgagaacg gtaaagctcc ccaaaggaaa gaaaatcttt gtgttctcag
     1741 ctcttgccgt taagaacgac attccgttca ctctcgccaa cgacctttat gacagagctg
     1801 agaagcgtga ttccttcggc tttgaagtcc ccgctgaggt acatgaggag gcaaagctta
     1861 cagctaaaga gaagaaacag gctcgcaaaa agtttgtgcg caattacacg aagaatcgcc
     1921 tccaaaggga aatcagcgca agaaagccat gataaaggag tattaatatg aagtttctta
     1981 aagccgcggc ggctgccctc ccgcttcttt tgctgctttc ctcctgctca aattcccata
     2041 aagccgcaaa ggacatcgcc gtcagggcgg ttatggatgt gcaaagctac gaaaacggca
     2101 gcattgtgga aatcccccgc ctttattaca acagcgaaag cagattttta tccgagcagg
     2161 gcggggaaat tcccgacatt aagctcatta acagcagcat tgaaaagagc tttaaggcaa
     2221 actacgagca gttccgaaag gagaagcgca agggctgcat tgatatacgc acctacccat
     2281 ttgacagcga gcgctatgtt cagcttgtca tcacctacaa cgagtacccc acctacggca
     2341 cggacggctt tttgcagagt ttctgctttg acaaattaaa gaacgagctt atcaccgccg
     2401 aagaaagact gaggcatgac agcatcaccc ttcccgagct taaagaaaaa gcaaaagagc
     2461 tttatacgcc ggaatatgag ggggatttcc tctccgacgc ctctctctcg ggctttgtga
     2521 ttcttgagga ccgcaccttg taccttatga cccttacagt cagaaacgaa aactccccca
     2581 cccgcatttt gttctattct ctcagcctgt ccgaaaactc cctaaagcgg cttgacaagg
     2641 atatgctgtt tgacaagggt ctcctttcac gccttgaccc gccgctttat tacgacaggg
     2701 aggagggcgc taataaaagg tcattcccct taaagctgat tctgaaagca gagaacctta
     2761 acgaaagcgg agaaaataag tacagccttg aaggcaaaac cgaaatcaca attaaaagct
     2821 cagagccagt gcccgtattc gcctcccata tctcagagct tgtaaagaac gccgagggcg
     2881 gcaaggctct tgacatcacc tgcgagtacg atttggggct tacggaaaaa atcgagtatc
     2941 ccgcctttgt tctcgagtat aaaaagcctt tgtacggcag ggagctgaga tgcctcgata
     3001 tggtgctggt taccgagtcg gcggcgttca gagttcacac cgaaaccgac gaaaaagact
     3061 attacagaat aaaagacaaa atcatagagg ctttcgggag cgcggatttt gtgtattagc
     3121 aagacaagca aaaatttaaa aacagacgca agcgtaattc gcgtctgttt ttttattgcg
     3181 gaaatttagc tttctgcaca agcgtcatac agaaaatttt ggataatcct aacagaaata
     3241 ctttggaact ttgtcaaatt ttgtagatgt tatggacaag ctgtcatttt tctgttatac
     3301 tcactccgaa ttggaaataa ttagggagtt tgacttcaaa gagcagtcaa aatttgcttc
     3361 ccaaagaaaa gaggagatta ttatggtttg ccccaagtgc aattccgaga ttatggacga
     3421 ggcggtgctc tgtcccgaat gtatgtgcag ggtaggcttt acaaaccgtc ccacataccg
     3481 cctcaaaacc aacaggagcc taattaagta catccttcta tccattgtca cattcggcat
     3541 ctactctttg attgcaatgt caggcgtcag ctccgacatt aacatcatag ccgaaaagta
     3601 cgacggcaaa aagacaatgc attttctcct tgtgaccttt gttctttcgt ggctgacctt
     3661 tggaatctac cccatagtat ggttccacaa gctctccgac cgcataggcg acgagctgga
     3721 ccgcagagga ataggctacg actttggcat gaaggattac tggctctggg cagtcttggg
     3781 ttcgctaatt gtaataggcc cctttattta cctgcataaa ctgcttgaaa gcatgaacct
     3841 cctctcagcg gactacaatg aaagaggtta acataaataa aaagccgcgc cttaacggcg
     3901 cggttttttt aattttttgc gcagatttcc agcaccagct ttttatagtg cctgcccttt
     3961 tcctcaaagc ctttatagag gttatagctt gccgccgcgg gagaaagaag cgccgccttg
     4021 ccctttggag taaagtcctt cgcgtgtctt acggcgtcct ccatgtcctt tgcgtaaatt
     4081 atgcggcttg tcgcgccctt tgactccatc agctttccta tgctgtatcc ggtgtcgggc
     4141 aggcagatta tattgtcaat gccggattta ataagctcat cggcaaagtc cgtatagtca
     4201 agacctctgt caagtccgcc gataatcaga cttcccacat ttcctaaagc ctcaacggcg
     4261 ctgagcacag ccttcggaat ggtggctatg cagtcgttgt agtactccac atcgtcaaag
     4321 gtgccaacat actccattct gtgctctatg cccgcaaagt tgtcaacagc tctctttatc
     4381 gcgtcgttgc ccactcccag gcagcgcaca gcctttgccg ccatcatggc gtcatggaga
     4441 ttgtgcctgc cctttaaatg cgggttgccc tttgtgagat tgtttaaaaa ttcctcgtcc
     4501 gacactgacg ggcaggggat tttctttgac tttacggagt caagacttat atactcctcc
     4561 aagttctggt cggggttata tataaagtaa tccttttcgg tctggtttct cacgatgttg
     4621 agctttgcgt ttacatagcc ctcaaagccg ccgtcgtagt ggtcaagatg ttcctcgtag
     4681 atgtttgtaa gaacggcgat atggggggag gtccttgtaa actggagctg atgggaggaa
     4741 agctcaatga ccgcaacggc ggagtcggaa atatctatat cctcaaagac gggtactccg
     4801 atgttgccga taagacaagc gtcaaagccc gcctctttaa gcatagagta aatcagcgtt
     4861 gatgtggtgg tcttgccctt ggtgccggtg attccgacgg ctgtaaagtc ggagaacctt
     4921 aaaaacaagt cggtctggca ggtaatctct gtgccgtcgg ggattttcac gcctttaaag
     4981 ggtatgccgg gacttttgag cacaaggtcg aaccgggcaa ggcaggagag gtaatcctcg
     5041 ccggtgatga gccttacatt atcgtcactc agctcaatgt ccttcatgtc ggcaattgca
     5101 aggggtttat tgggcagcac ccttcttata aactcatagc tggacctgcc ctccttgcca
     5161 aagcctaaaa tgaggatgtt tttatcctcg aaatattctt taagcctcct gcttgtgttg
     5221 gacaaaggaa tacatctccc ttacggtgtt cataaatttt tcgggtatat tgtgctcact
     5281 gttatagtgg gaaagtatgc tctctatatc gggaaccttg tacggggcgc tgtcgccgct
     5341 gaagcactct ttgtgccgcc ttaaaagggg cggcagtgct ctgcccgctg actcgcactg
     5401 cctaacggtc tcgtttaaag ccgcggcgac ctcctcaacg gtgccgacgc actcaaaggg
     5461 tttcaatttt atgaccccat tgagtccgtc gagaatctcg cttaaatgct ctttttcgaa
     5521 aaggtcagag ccgaaaattt ctatcagctt ctcattcggc aaaaagggcg agagaatcga
     5581 gtaaacaaag aggcattttg cgcactcgcc gcaccagatg ttctgtttgc tgcctctgtt
     5641 gcagcttctg aaaatgctgt ggaactgagg caaatccgaa aattctttcg cgatctgcag
     5701 ctcattgaag ggtcttaaca tactgaaata ctttattttc tcggtgaaat tcctctttgt
     5761 aaactcgaag aaatcccgct cgaactcata ggacttggaa tactgatgat ttacctcgct
     5821 gccctctata ttgggggcgt tggcgcttgc ctcgtttgag agaacaatat gctccgcgcc
     5881 gatgatatat gcgcagtaat acgaaagaaa agccacaatg gcggagaagg gggtatgtcc
     5941 gtttaaaaag ccctttttgt taagctccaa aagctcgggg tctatcttgc ggtatgttct
     6001 gactattctg tcttcgccgt agcccgcggc ggtgacggtt tcggtcctcg ccttttggtc
     6061 gtttatagta aagaacatat tcttgtccct gtgatgcctg agcagatttg ccgtaaccgc
     6121 cgaatccttg cccccgccca cggggataag atttatttct gaggatttga attcttcgct
     6181 ttctgcgcct tcaaaatcct ccccgccctt atattcaaag ctcaaaagct tatccttttc
     6241 tatgtttata ttattggtat aaaagaactc ggacaaaccg ttatggtaca gcttaatgaa
     6301 aaattcctca tcagccttgg tcatttttcc gcaggagata ataaccttcg ggctcaatac
     6361 cgccttatag tagctgacaa gctctgtcat gccgataaag aaggcgattt ttctcgccat
     6421 ggggctaaag gggtcgttga ggattttcaa attctccgtc acaatcagcg tatcggggca
     6481 aaaacgacaa agcccttcta tctcaaaatc gaaagaaagg cgaattcccc cctcagcttt
     6541 ttccaagctg aaggagttat atttaaatac aggatatttc tctctcaaag cattataatc
     6601 tttagcttcc atagactctc cgttttaagt ccttttgtta cacatagttt agcacattcc
     6661 tttatttaac tccataccga gtttaaactt taataaaaaa gggggaggcg agggggtccc
     6721 cgcccccctt taatatgttc agataaacga agacatacct attgttaata tgcctatatt
     6781 gtcgttatat attttgtcga attggctgag cggcagcggg ctttcgccga gacccacctc
     6841 caccgtatag ccggggcggt tgaactcctg aatgaaccag tctttgtagc cggcgtagcc
     6901 tgagtactcg ggggtaatct ccaagggata gccgcttgcc tgactcattc tctcgccaat
     6961 ctcccttgcc ctgggcgggt catagtccat gaacttccag tagattatct caccctgggt
     7021 atggtaggcg agaatcaggg caaagtcatg gtttagggta aagttgtaaa ccgcggcgct
     7081 ttcgggagct gacaatggag cggagccgac ataatccctc ggagcggggg atacaaagcc
     7141 cgccgcgaac ttttgctccc ttgccctctc ccattcggcg gggtagttaa gatttaagtc
     7201 ggtgccggct atatttgcct tccagcccga agggaagggg atgttcgggt aatccgagga
     7261 aatcctgaca gcgttctcat agaaggttcc tcggttaatg gctcccgtta caaggtccac
     7321 tccgtcgggg tttacaaggg gagccacata aagggtggtg cctctgtata ggtcggcggc
     7381 tcttgtgccg aatatgctgc cgttttcgga ataagctctt gcgtagtcct ccacatactt
     7441 tagaagaacc ggagttgtaa tccactcgtt ggcgtggtgg gcggcgttgt agaaaacctg
     7501 agtggcgccc gtgcctatgg atatgtagtg caggctcttg cccatgacgc ttctgccgaa
     7561 gttgcccgtt ctcaaaaagg gatatcttgc ctttaagccg tcaataataa gagtagtgag
     7621 ggtcgaggtg taatcgatgt ttgtcgacac cgccgggaaa ccgaagggta ttactatggt
     7681 ctgacccggt cttagactgt cggggtccaa agtggggttg gcggttagaa tagcccttac
     7741 cgtcgtgccg tactgcctgg caaggctcca tagtgtgtcg ccgcttctga tgaccctgta
     7801 aaagtagcct cttatgtatg gcagtatatg tccccatgtg gcgggaccca caacgccgtc
     7861 ggaggtaaca ccgaaggcgc tctggaaact gaataccgct ctttgagtct gatagccgaa
     7921 gattccgtca atttctccgc tgtaatagcc tgcccgagca agggccaatt gcagcatttg
     7981 aaccagcggc cctcgcatcc caagtctcaa aacttccaaa acgaccactc ctaaacatag
     8041 ttctatctaa agtatatggc gcattgtctg tcctcaattc cgactaatat gcgaaaaggg
     8101 ggaaactttg ccgaaaacga gccttacatt aatttcagcg cgaattttaa atttttctcc
     8161 gttttgccca aaaatggatg aaagtttaaa aattttccgt tgcagacgat gacaaaaaca
     8221 taagattttg cacaaaaaag cggcatagaa tccatgcctt ttaacaaatt aaagcatttc
     8281 ccttgaaaat tctcagaaaa taagctataa tttgtttgta attaatgttt aaattgcgca
     8341 cattctgccc ttttttgcat aaaatattca tttggagatt tttttatttt tggcacaaag
     8401 gcaggaatcg cgtaggaggg ttattttgtt agcggattta cattgtcata ccaaactgtc
     8461 ggacggttcg atgggtattg aggagctgat tcttcttgcc gcgggccgcg gcgtttcaac
     8521 catcgcgatt acggaccatg actgccttgc gggaaatgtg agaggaaagg tcataggcga
     8581 gcgtcacaat gtcagagtca tccccggcgt tgagctttcc gccacagaca ctctaacggg
     8641 caacgaggtc catattttag gctatctctg cgactgtccc gacagaatag aggggctttg
     8701 ccacaagaac tccatggcca gaaggcgcgc gggccagtat atgatgctta aagcggcaaa
     8761 gaagttcccc atatctcccg agttcgtttt aaggtgcgcc cagggctcca ccaacatatt
     8821 caagcagcac attatgcacg cccttatgga gataggctat tcctcaagca tgtacggcga
     8881 gctttatgaa ctgctctttt ctcccgagag cgaggagaac atcatagtca acgccaagta
     8941 ctcccccgtt gaggaggtta tcgcagcgat tcactccgcg ggaggaatcg cgatacttgc
     9001 caaccccgct gtttacggcg gtcttgagct tgcagaaagg atgcttgagc acggtcttaa
     9061 cggcattgag gtatggtcct ctgacgccag cgaggaggac acaaagaagt tttacgaatt
     9121 cgctaagaaa aacaagctcc tcatgaccgg aggcagcaac ttccacggaa tgtataacaa
     9181 gggcatttgg agcgtaggcg actacggagt gcacgaggac caggttcagg agcttttgca
     9241 gtacaaggca aggctcaggc gtcagcaaag caagaccgag acaaaacagg aagttaccgt
     9301 ttagaaacca ccaccgccac aaggcaaagg cgccgcttgg caaattgccc gaggcggttt
     9361 ttttataaag aagccccttg catttttacg gaatttcccg catattgtaa ttaggacaaa
     9421 gagtttaggg ggaagagcat gagaataaat gacgacagcg atataaaaat ctttggggaa
     9481 aagaaaaaga aggacgactt tgacccgatg cttctgctgg aggagataaa gcgccaccgc
     9541 agcagcggca acaccgctaa ggcaaagaca atcgcccatc acatggcgga cagcattgtg
     9601 gaaagtgaca tagacgagtc cgtaaagagc tttgcggaca ggtacgaagt tgaactgaca
     9661 agcgaagtca tgcatcaggt cagggtgctc tccgtctttt ccgccgagta tgtgctgaat
     9721 aaataccttc cctcacccct tctctcctcg gtcgcgataa acgagcttta tgagagcata
     9781 ctcgcagagg ctccggagtt ttacgacaga cttgaaagcg gcaccgcgtt tactttttat
     9841 tatctcgcag tcagaagcgg cggcaacacc cccgccgaaa tcggcaaggc cttcgccagc
     9901 cagtgcggga gagaggacga cggcagctgc gcacttttcg gcaccgccgt tttcaccctg
     9961 aatgtggaga ctttccagaa tcttatattc agctttaatt ttgaggaatg aaacccttta
    10021 aaagtcagaa caagagcagg cattcagcct gcttttttta ttattgtttt cacccgcccc
    10081 cgcaaaagag ctcgccgccg cgtggaaacc cacacgccgc accgcccccg caggggcgaa
    10141 ctcaaccacc ctgcccgccg tggagagggc gcaagcgcca ccacagagcc caccccagac
    10201 cgaactcaac tcagccgccg ctgcactccc cgccgcgccg caggagtgaa ccccgccctc
    10261 cttcctgcta agaggcgtgg aacgccgaga gagcgccgcc gcgcgggaat gcccgccaca
    10321 ccttgcgcag aagggcgcac acgccaaaca
//
LOCUS       DAONVQ010000068        10148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5077_length_10148_cov_3.968394, whole genome shotgun sequence.
ACCESSION   DAONVQ010000068 DAONVQ010000000
VERSION     DAONVQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5077_length_10148_cov_3.968394"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLD98_05505"
     CDS             <1..719
                     /locus_tag="PLD98_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05740.1"
                     /translation="TAMPIETGKQYKGSISERSRRTDKDFFKVTLPMDGSVALLFEKE
                     PINDQKDGWNITLINKDGEVMYSAVAKLSTAKIESPRIGLAAGEYYVVIDSENRTLNT
                     APYTISLDFDATYYWEAEKNDTMAKANPISVNRDYRGSLVEIGIDFDTDWYSFELTGE
                     SRVSVSFSHNKIEPYLNKGWLIQLVGKDGRVIKSVTSRWDDAVVSTEEAVLPAGKYYV
                     KVDTAEYYNSATYILKVNAQ"
     gene            771..2048
                     /locus_tag="PLD98_05510"
     CDS             771..2048
                     /locus_tag="PLD98_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQA05741.1"
                     /translation="MGIIKVLTSAIGGGLADSWLEVFEADNMGDNTVMTKGVAVRRDS
                     KRNRNIKGEPKAVSNGSIIHVYPNQFMMLLEGGKVVDYTAEEGYYRVDNSTLPSLFNG
                     ELSESVKETFRRVEYGGTQPSAQVVYFINLQEIKGIKFGTKNPINYFDTFYNSELFLR
                     AHGTYSIRITDPIKFFYEAMPRNKDRVDIQDINEQYLSEFLTAFQAAINRMSAEGQKI
                     SYVTSKSMEISKYMSEVLDEDWKATRGFEVQSVGIASISYDEESMKLINMRNRGAMMS
                     DPTIREGFVQGSVARGIEAAGSNQSGAMAGFLGVGIGMQAGGGFVGEASRSNQAQMER
                     ESAKSANMWICPECKAENEGKFCQNCGKARPNGGGWVCSCGTENGGNFCINCGKPRPT
                     NDFKCSNCGYEVKGIAQKIKFCPNCGKPNEQNS"
     gene            2072..3139
                     /locus_tag="PLD98_05515"
     CDS             2072..3139
                     /locus_tag="PLD98_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HQA05742.1"
                     /translation="MAVVSYKCPNCDGPLVFRPRENDFYCDFCQSRFNKSEVEAGNSG
                     AAETVSAAPKTQITEDAELFEKEAVTYSCPSCGAEIITAESTAATQCYYCHNPVILSG
                     KLSREFRPDRLIPFAISKDEAIKRFKDFCAKKRMLPKEFLTEGTFESVTGVYYPYYIV
                     DAKIFGQATAKSKTTRTWIAGNFQYTEISDYRHERKGDLDVEGIAVSAYKDGDRNLLK
                     YVCPFDESKSVDFAMMYLSGFQAEKRQLGKADLYGEIEKKLRDIAENQFKGSINAGGA
                     ITSFEMKINDLKDHWEYFLFPLWVMTYNYRGKLYRYAINGQTGKLYGELPVDKLKLFT
                     TAAVSALSVLAAILIYGGFAA"
     gene            3136..4062
                     /locus_tag="PLD98_05520"
     CDS             3136..4062
                     /locus_tag="PLD98_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQA05743.1"
                     /translation="MKNKARNNIKFLIITLSMALVVILNFSILSFADTLDFYVVDNAG
                     LFSNSEINILNEAIKNSADDAPYLFILTINDAKGKTAMDYADDYHDELIDKKGFPADS
                     ALFLIDMDNRELWVSTTGTFIDKISGGNLDYLLDSAQFYAKNGSFYEAAESFVDISIG
                     IISGSIDVERPKEDYGYYPGFPDFDEDLIVPRNPTLKDQYFLGIRLDFIIAAFFAALI
                     TFGAIVIYTFVSYKTIGKSGDANMHRARLNIRHQSDVLVGRHVVTTPIKTHTDSKGGS
                     SHKGTGTGMSFGGSISHTSSAGRSHGGGGRGF"
     gene            4084..4497
                     /locus_tag="PLD98_05525"
     CDS             4084..4497
                     /locus_tag="PLD98_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05744.1"
                     /translation="MGKDKKDKVKESIDSLIEIKAPKASHTKGFYRFSKFFPPAVNEE
                     GFRDNDFLRKEPMSLRAKWLLAVGAVIVFCVSFVLISTAMMLSKKEPEPTTALNFENE
                     FYTEPSASDISAETTDTDDPTSSEQTDTEVESGVE"
     gene            4586..4765
                     /locus_tag="PLD98_05530"
     CDS             4586..4765
                     /locus_tag="PLD98_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HQA05745.1"
                     /translation="MSNNLARYTLRVNRSLLYKLGYIAGSEGRTKNKELEQLIKKRIA
                     EYEAQYGTIDLPDDY"
     gene            complement(4814..5740)
                     /locus_tag="PLD98_05535"
     CDS             complement(4814..5740)
                     /locus_tag="PLD98_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQA05746.1"
                     /translation="MRKYLPEGSLINTAENKAAMENEEALKRAMLSGTILEARAVLCD
                     KEHNLHVNLGCMRGIIPRKEAALGIEEGTVRDIAIISRVNKPVMFKVLEIEENSFGTK
                     TAILSRRVVQEEFKEYYTKEWSVGDIIDAKVTHMESFGAFCDIGAGISALMPVDSISV
                     SRIPHPNVRFSVNQNIKAIIKSFDSEGRVNLSHKELLGTWLENASMFSVGETVPGIIR
                     SVENYGIFIELTPNLAGLAEYTEGVKEGEWASVFIKSIIPEKMKIKLIIVDSFEADYP
                     PLPIKYFYTEDKIKHFLYSPPGCEKVIETYFE"
     gene            5894..6094
                     /locus_tag="PLD98_05540"
     CDS             5894..6094
                     /locus_tag="PLD98_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HQA05747.1"
                     /translation="MDIKVGDTLVLKKNHPCGGNSFYVLRIGMDFKLKCLSCSREFMI
                     PRSKAEKAVKQIIKAQDTINPG"
     gene            6164..7237
                     /gene="prfA"
                     /locus_tag="PLD98_05545"
     CDS             6164..7237
                     /gene="prfA"
                     /locus_tag="PLD98_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQA05748.1"
                     /translation="MLDRLENVEKRFEELDEKLCSPDIATDIEQYTKLMKERKQLLPI
                     VEKYREYKEYKKTFDEAAELLQSKGLDKDFREMVEEEYKNSKEALERCNEELKILLLP
                     KDPNDDKNVIVEIRGGAGGEESALFAGVLFRMYSMYAEAKGFKIEVLNANETGLGGYK
                     EIIFMVSGEGAYSRFKFESGVHRVQRVPETESQGRIHTSTVTVAVLPEAEEVDIEINP
                     ADLQIDTFRSSGAGGQHVNKTESAIRITHIPTGTVVECQDERSQHKNREKAMRILRSR
                     ILEEERRKQTEEIAGQRKTQVGTGDRSERIRTYNYPQGRVSDHRIGLTLYRLEEVLNG
                     DLDELIDALITADTAEKLQMQEN"
     gene            7248..8324
                     /locus_tag="PLD98_05550"
     CDS             7248..8324
                     /locus_tag="PLD98_05550"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQA05749.1"
                     /translation="MLKTEVLSAINVSDAEYKRIIEKAAETVKRGSVAALPTETVYGL
                     FANALSGEAVRKIFDAKGRPQDNPLIVHIADFEDVYKLSDDVPIEAKLLAEKFWPGPF
                     TMILKSNGKVAKEVSAGLSTVAIRMPDSKIALDVIRESKLPLAAPSANISGFPSPTSA
                     EHVIDDMTGRIELIIDGGDCRFGVESTVLSLTGDVPKILRPGAVTAEDIKSVVGAVEI
                     DESVLKPLAEDAKVSSPGMKYRHYSPKAQVIVLKGSFKEYLDYINNERKDGDFALCFD
                     GEEDKLPINAVSYGAENDSLAQAQRLYGALRQLDDMGAKRVFARCPSKEGVGLAVCNR
                     LFRAAGFNVVDLEKALQAGFLWQR"
     gene            8312..8902
                     /gene="coaE"
                     /locus_tag="PLD98_05555"
     CDS             8312..8902
                     /gene="coaE"
                     /locus_tag="PLD98_05555"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059954.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQA05750.1"
                     /translation="MAKIIGLTGPIGAGKSTVARILSEKGCVIVDCDKIAREIVEPGS
                     PVLSVLAKEFGNDILNEDGSLNRPRLAEVAFSSKENQLKLNSITHPEILKVALSRATK
                     AIKEGKNAVIEAALLFESGANMLCSASVAVVAPKEERKKRVAARDNLDEKAIELKMNV
                     QKEESYYTERADFVIRNHEPYDINYELQPLFKYLGI"
     gene            9074..9508
                     /locus_tag="PLD98_05560"
     CDS             9074..9508
                     /locus_tag="PLD98_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQA05751.1"
                     /translation="MEKYAADYGVDKALIYAVIRTESSFNASAVSYAGARGLMQMTPD
                     TFKWLQTKTGEKLDTEMLFDPETSIKYGTFFLNMLLEEFGELETAIAAYHAGRGKVNS
                     WLRDSAISPDGKTIPEIPIPQTRHYVNKVMSAYERYKKIYEF"
     gene            9526..>10148
                     /locus_tag="PLD98_05565"
     CDS             9526..>10148
                     /locus_tag="PLD98_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HQA05752.1"
                     /translation="MSKEKSAADKLREELLVQNKHMAKVVSDDEINKAFEFSKGYMSF
                     MKKAKTEREAVKEIEKMAYEKGFEPFVPDKKYKPGDKVIYNNRGKAVILAVIGKKSIK
                     EGVRISAAHIDSPRIDLKPNPLYEDSELALFKTHYYGGIKKYQWTAIPLSLHGVVIKA
                     NGEKVEITIGEAEGEPKFVITDLLPHLGQAQSQKKLGEAIKGEDLNIV"
BASE COUNT         3035 a         2256 c         2422 g         2435 t
ORIGIN      
        1 aaaccgcgat gcccattgaa acgggcaagc agtataaggg ctcaatttcc gaaagaagcc
       61 gcagaaccga caaggacttc tttaaagtga ctctgcccat ggacggttca gttgctctgc
      121 tctttgaaaa ggagccaata aacgaccaga aagacggctg gaatatcacg ctcataaata
      181 aagacggcga ggttatgtat tccgctgtcg caaagctcag caccgcaaaa atagagtccc
      241 ccagaatcgg acttgcagcg ggcgagtatt atgtggttat cgactccgaa aacagaaccc
      301 tcaataccgc cccgtacaca atttccttag attttgacgc cacctattat tgggaagctg
      361 agaaaaacga tacaatggca aaggcaaatc ccataagcgt taacagggat tacagaggct
      421 cccttgttga aatcggaatc gactttgata ccgactggta cagctttgag ctgacagggg
      481 agagcagagt atctgtcagc ttctctcaca acaaaataga gccttatctt aacaagggct
      541 ggcttattca gcttgtcggc aaggacggca gggtaataaa atcagttaca tccagatggg
      601 acgacgccgt tgtctcaacg gaggaggcag tcctccccgc aggcaagtac tatgtaaaag
      661 tcgatacggc cgaatactac aacagcgcga catatatttt aaaggttaac gcccagtagt
      721 tttgttcttt attctaatca aaataaatac tatttttatg aggtgttgtc atgggtataa
      781 ttaaagtctt gacaagcgca atcggcggag ggcttgcgga ttcttggctt gaggtgtttg
      841 aagccgacaa tatgggcgac aatacggtta tgacaaaagg cgtcgctgtc cgcagagatt
      901 caaagcgcaa cagaaatatt aaaggcgagc ccaaagccgt atcaaacggc tccataatcc
      961 atgtctatcc caatcagttt atgatgctcc ttgagggcgg caaggttgtg gactatacag
     1021 ccgaggaggg ctattacagg gttgacaatt ccacacttcc gtcgcttttc aacggcgagc
     1081 tttcagaaag cgtaaaggaa accttcagaa gagtagaata cggcggaaca cagccctccg
     1141 cgcaggttgt ttactttata aatctgcagg agattaaagg cattaagttc ggcacaaaga
     1201 accccattaa ctattttgac actttctaca attccgagct tttcctcaga gcacacggaa
     1261 catattccat acgcataacc gaccccataa agttcttcta tgaggctatg ccgagaaata
     1321 aagacagggt cgatattcag gatattaacg agcagtatct ctccgagttc cttaccgcgt
     1381 tccaggctgc catcaacaga atgtccgccg agggtcaaaa gatttcctat gtcacatcta
     1441 agagcatgga aatcagcaag tacatgagcg aggttcttga tgaggactgg aaggcaacca
     1501 gaggctttga ggtccagtct gtgggcatag cttcaatcag ctatgacgag gagtccatga
     1561 agctcatcaa catgaggaac cgcggcgcaa tgatgtcaga tcccacaatc agagagggct
     1621 ttgttcaggg ctccgttgca aggggtatcg aggctgcggg ctccaaccag tccggcgcaa
     1681 tggcaggctt cttgggcgta ggcatcggta tgcaggcagg aggcggtttt gtgggtgagg
     1741 catcccgttc caatcaggct cagatggaaa gagaaagcgc aaagagcgcg aatatgtgga
     1801 tttgccccga atgcaaagca gaaaacgagg gcaagttctg ccagaactgc ggcaaagcgc
     1861 gcccgaacgg aggcggctgg gtttgctcat gcggcactga gaacggcggc aacttctgca
     1921 ttaactgcgg caagccgaga ccgactaacg acttcaaatg ctcaaactgc ggctatgagg
     1981 taaagggtat cgcgcagaaa atcaaattct gccccaactg cggcaaacct aacgaacaga
     2041 attcatagcg tcatttcaat agggggatat catggcagta gtttcatata aatgccctaa
     2101 ctgcgacggt ccgcttgtat tcagaccgcg cgaaaatgat ttttattgcg atttttgtca
     2161 gtcccgcttt aataaaagtg aagttgaagc aggcaactca ggcgcagcgg aaacggtttc
     2221 tgccgcgcct aaaactcaaa taacagaaga tgccgagctt tttgaaaaag aagcggtcac
     2281 atactcctgc ccgagctgcg gcgcggagat aatcacagcc gagagcacag cggcgactca
     2341 gtgttattac tgccataacc ccgttattct ttcaggcaag ctgagccgag agttcagacc
     2401 cgacaggctt attccctttg ccatctctaa ggatgaagcg attaagcgat ttaaggactt
     2461 ttgcgccaaa aaaagaatgc tccccaagga gttcctgaca gaaggcacct ttgagagcgt
     2521 tacgggcgtg tattaccctt attacattgt tgacgcaaag atattcggcc aagcgaccgc
     2581 aaaatccaaa acaacaagaa catggatcgc tggcaatttc caatacaccg aaatctccga
     2641 ctatcgtcat gaaagaaaag gcgaccttga tgttgaggga atagccgttt ccgcctataa
     2701 agacggcgac aggaaccttc ttaaatatgt ctgccccttt gatgagtcaa aatctgtaga
     2761 tttcgcgatg atgtatcttt cgggctttca ggcggagaaa aggcagcttg gcaaagccga
     2821 cctctatggc gagattgaga aaaagctgag agatatagcc gaaaaccaat tcaagggctc
     2881 aataaacgca ggcggcgcaa tcacctcctt tgaaatgaag ataaacgact tgaaggacca
     2941 ctgggagtat tttctcttcc ccctttgggt catgacctac aattacaggg gaaagctgta
     3001 ccgctacgcc ataaacggtc agacgggcaa gctctacggc gagctgcctg ttgacaagct
     3061 gaagctgttt accacagcgg cggtatcggc gctttctgta ttggcagcaa tcctgattta
     3121 cggaggattt gccgcatgaa aaacaaagcc cgtaacaata tcaaattttt aattattact
     3181 ctgagtatgg ctttggttgt tatcctgaat ttctcaatac tttcatttgc cgatacctta
     3241 gatttctatg ttgttgacaa tgcgggactt tttagcaatt cggagataaa catcctgaat
     3301 gaagctatta agaattccgc ggatgatgcg ccgtatcttt tcatcctgac aatcaatgac
     3361 gctaaaggca aaaccgccat ggattatgcg gacgattacc acgacgagct gatagataaa
     3421 aaaggttttc ccgctgacag cgccttattt cttatagata tggacaatcg tgagctttgg
     3481 gtatccacca caggtacctt tattgacaaa atcagcggcg gtaatcttga ttatctcctt
     3541 gactctgctc agttttacgc aaaaaacggc agcttttatg aggccgccga aagctttgta
     3601 gatatctcca taggcataat ctcaggctct atagatgttg aaagacctaa agaggactat
     3661 ggatactatc cgggttttcc tgatttcgat gaagatttaa ttgttccgag aaaccccact
     3721 ttaaaagacc aatatttctt aggcataagg ctcgatttca ttatcgcagc attctttgcc
     3781 gctcttatta catttggcgc aatcgttatc tatacttttg taagctataa aaccatcggc
     3841 aagtcgggcg acgctaatat gcacagagcg aggctcaata taaggcatca gtccgatgtg
     3901 cttgtgggac gccatgtggt aacaacgccg attaagacgc acacagacag caaaggcggg
     3961 tcatcccata agggcactgg cacgggcatg agctttggcg gctccatatc tcatacttca
     4021 tcggcgggac gctcccacgg cggtggcggc cgcggcttct gattaaactg acggagatgt
     4081 tcaatgggca aagataaaaa ggataaagta aaagaaagta tagacagctt aatcgaaatc
     4141 aaagctccga aagcatctca taccaaaggc ttttatcggt tttcaaaatt tttcccgccc
     4201 gccgtaaatg aagaaggctt tagggacaac gactttttaa gaaaagaacc gatgagcctt
     4261 cgggcaaaat ggctgcttgc ggtcggggct gtgattgtgt tctgcgtttc tttcgtcctt
     4321 atttccaccg ccatgatgct ttcaaaaaaa gagcctgagc cgacaaccgc tttaaacttt
     4381 gaaaacgagt tttacactga accctctgcg tctgacatta gcgcagaaac gaccgatact
     4441 gatgacccga cctcatcgga gcaaacagat accgaggtag agtcgggcgt cgaataaagg
     4501 attttatgaa attattacct tatgtttttc ggttatatga aggcaaacta atgtcaatac
     4561 ttcttttacg ggaggttgac attagatgag caataatctt gcgcgatata ctttaagagt
     4621 taatcgttcc ttgctgtata agcttggata tattgcaggc agcgaaggca gaaccaaaaa
     4681 caaagagctt gaacagctta taaaaaagag gattgccgaa tacgaagctc agtacggcac
     4741 cattgacctg cctgatgatt attaaaaatg aaccccattc tccaaaagga aaatggggtt
     4801 taatttatgt agactactca aaatatgttt caataacctt ttcgcagccg ggcggggagt
     4861 ataaaaagtg ctttatttta tcttctgtgt agaagtattt aatcggcaag ggaggatagt
     4921 cagcctcaaa gctgtcaact ataatcagct taattttcat tttttcgggg ataatgcttt
     4981 ttataaagac gcttgcccat tcgccctctt ttacgccctc cgtatactcg gcaagcccag
     5041 cgaggtttgg cgtaagctca atgaaaatgc cgtagttttc aactgacctt ataattcccg
     5101 gcaccgtttc tcccacagag aacatggagg cgttttcaag ccatgttcca agcagctctt
     5161 tgtgggagag attcaccctg ccttcactgt caaagctctt gataatcgcc tttatgtttt
     5221 gatttaccga gaaccttaca ttcgggtggg gaattcttga taccgatatg ctgtcaacgg
     5281 gcatcagcgc gctgattccc gcacctatat cgcaaaaggc gccgaagctc tccatatgag
     5341 taaccttggc gtcgattata tcgcctacgg accattcttt ggtgtagtat tccttaaact
     5401 cctcctgaac cactcggcgg cttaaaatag ccgttttagt gccaaagctg ttctcctcaa
     5461 tctcaagtac cttaaacata acgggcttgt ttacccttga gataatggca atatccctta
     5521 ctgtgccctc ctcaatgccg agcgcagcct cttttctggg aataatgcct ctcatgcatc
     5581 ccaagttaac atgcaggttg tgttccttgt cacaaagcac agctcttgct tctaaaatag
     5641 ttcccgagag catagctctt tttaatgctt cttcgttttc catagccgcc ttgttttcag
     5701 cagtattgat aaggctgcct tcgggcaagt attttctcat atttgtaacc gtcccttcaa
     5761 cagatggtaa gtcccttgat ttagctcaga caaaaacttt atacattaat taataagtat
     5821 ttatcttagt ggaaaagtat gatacaatat ctgaatgaaa ttattgctaa aacatttcgc
     5881 atttggagga aaaatggaca taaaagtcgg ggatacgctt gtgcttaaaa agaatcatcc
     5941 ctgcggaggc aatagttttt atgttctcag aatcgggatg gactttaagc taaagtgcct
     6001 tagctgcagc agggagttta tgataccgcg ctccaaggca gaaaaggcgg taaagcagat
     6061 aataaaagcg caggatacaa taaatccggg ctaacctcag ctaagttttg ctaaagataa
     6121 gaaataatat gacagtaaaa tactatgaag caggagataa tttatgcttg acaggcttga
     6181 gaatgttgaa aagcgatttg aagaattgga cgaaaagctc tgcagtcccg atatcgcgac
     6241 ggacattgag caatatacaa agctgatgaa ggaaagaaag cagcttttac ccatagttga
     6301 aaaatacaga gagtataagg aatacaaaaa gacctttgac gaagcggcag aactgctgca
     6361 aagcaaaggt cttgacaagg atttccgcga gatggtggag gaggagtata aaaactccaa
     6421 agaagctctt gagcgctgca atgaagaatt aaagattctt ctcttgccca aagaccccaa
     6481 cgatgacaaa aatgttatcg tggaaatcag aggcggcgct ggcggcgagg agagcgctct
     6541 ctttgcgggt gttcttttca gaatgtactc catgtatgcc gaggctaagg gtttcaaaat
     6601 cgaggtcctc aacgccaacg aaacaggtct tggcggttac aaggaaataa tattcatggt
     6661 cagcggcgag ggcgcatatt cccgctttaa gtttgaaagc ggcgtccaca gggtccaaag
     6721 agttcccgag accgaatctc agggccgtat ccatacctca accgttaccg tagcagttct
     6781 gcccgaggct gaggaagttg acatagagat taaccccgcc gaccttcaga ttgacacctt
     6841 caggtcaagc ggcgcaggtg gtcagcatgt taacaagacc gaatccgcaa taaggattac
     6901 ccatattccc acaggcaccg tagttgagtg tcaggacgaa agaagccagc ataagaacag
     6961 agaaaaggca atgaggattc tccgctcaag gattcttgag gaggagagaa gaaagcagac
     7021 cgaggaaatc gcaggtcaga gaaaaactca ggtcggcacg ggcgacagaa gcgaaagaat
     7081 cagaacctac aactatcctc agggcagggt aagcgaccac agaatcggtc ttactctcta
     7141 ccgtcttgag gaggttctta acggcgacct tgatgaatta atcgacgccc ttatcaccgc
     7201 cgacaccgct gaaaaactgc aaatgcagga aaattagagg aatcataatg ctaaaaacag
     7261 aggttttaag cgccataaat gtttccgacg ctgaatataa aagaataata gaaaaggcgg
     7321 cagaaaccgt aaaaagaggc tcagttgccg ctcttcccac tgaaaccgtt tacggtctat
     7381 tcgctaacgc tctgagcggc gaggctgtaa gaaaaatctt tgatgcaaag ggcagaccgc
     7441 aggataatcc ccttatagtc cacattgctg actttgagga tgtttataaa ctgagcgacg
     7501 atgtgcccat tgaggcaaaa ctccttgccg aaaaattctg gccgggtccc tttacaatga
     7561 ttctcaaaag caacggcaag gttgcgaaag aggtcagcgc agggctctcc acagtggcga
     7621 ttaggatgcc tgacagtaaa atcgccctcg atgtcataag agagtctaaa ctgcctttag
     7681 cggcgccctc agcaaatatt tcgggctttc caagccccac aagcgcggag catgttatag
     7741 acgacatgac gggcagaata gagcttatta tcgacggggg agattgcaga ttcggggtag
     7801 agtccaccgt tctgagttta acgggcgatg ttccgaaaat tctccgcccg ggtgccgtta
     7861 cggcggagga tataaaatcc gtcgtgggag cggtggagat tgacgagagc gtattgaagc
     7921 cccttgcgga agacgcaaag gtttcttctc ccggcatgaa atatcgccac tattccccca
     7981 aagcacaggt cattgttctg aaaggcagct ttaaagaata ccttgactat ataaacaatg
     8041 agcgtaaaga tggcgatttc gccctttgct ttgacggcga ggaggataag ctccccatta
     8101 atgccgtaag ctacggagct gaaaacgaca gcctcgctca ggctcaaagg ctctacggcg
     8161 ctttaaggca gcttgacgat atgggagcga aaagagtttt cgcccgctgt ccttcaaagg
     8221 agggcgtagg ccttgccgtc tgcaacagac ttttcagggc ggcaggcttt aatgtggtgg
     8281 atttagagaa agctttacag gcaggttttt tatggcaaag ataataggtc ttacgggtcc
     8341 aataggagcg ggaaagagta cggttgcccg cattttgagc gaaaagggct gcgtcatagt
     8401 tgactgcgac aaaatcgccc gtgaaattgt cgagccgggt tcaccagttc tatccgtact
     8461 ggcaaaggaa tttggcaatg atattctgaa tgaagacggc agccttaaca ggcctaggct
     8521 tgccgaggtc gcattttctt caaaagaaaa tcagctgaaa cttaattcca taacccaccc
     8581 agagattctt aaagtagctc tcagccgcgc cacgaaagcg ataaaggaag gcaagaacgc
     8641 ggtaatagag gcggctcttc tttttgaaag cggcgcaaat atgctgtgca gcgcaagcgt
     8701 cgccgttgtc gcccccaaag aagagcgcaa aaagagggta gccgcaaggg acaaccttga
     8761 tgaaaaagcg atagagctaa aaatgaatgt tcaaaaggaa gaaagctact ataccgaaag
     8821 ggcggatttc gtaatcagaa accacgaacc ctacgatata aactatgagc ttcagccgct
     8881 gtttaaatat ctcggtattt aaaacagcgc tttaggtgat gaaatgaaga tattaacaat
     8941 aaaaaataaa aaaatgctct atgtttatgc attattatgc ataattattc ttgctttgct
     9001 tattgcaatc ctttatccat ggtggtataa taggcgtcag atgagagaat atccgcttaa
     9061 acatacagaa ttagtggaaa aatatgcagc ggattacgga gtcgacaaag cccttattta
     9121 tgcggtaatc agaaccgaaa gcagctttaa cgccagcgcg gtttcctacg cgggcgcaag
     9181 gggtcttatg cagatgaccc ccgacacctt taaatggctc cagacaaaaa cgggcgaaaa
     9241 gcttgatacc gagatgcttt tcgaccccga aacctcaatt aagtacggta ctttcttcct
     9301 taatatgctg cttgaggaat tcggcgagct tgaaaccgcc attgcggcat atcacgcagg
     9361 ccgcggcaag gtaaactcat ggctcagaga ctcagccatt tcccccgacg gtaaaacaat
     9421 acccgaaatc ccgattccac aaacaaggca ctatgtgaat aaagttatgt ctgcctacga
     9481 aagatataag aaaatatatg aattttaaaa gaacggaggc tctgtatgtc taaagaaaaa
     9541 tcagctgcag acaagctgag agaagaattg ctggttcaaa acaagcacat ggcaaaggta
     9601 gtaagcgatg atgaaataaa taaagccttt gaattcagca aaggatatat gtcctttatg
     9661 aaaaaggcaa agaccgagcg cgaggctgtt aaggaaatag aaaagatggc atacgaaaag
     9721 ggttttgagc cttttgtgcc cgacaaaaag tacaagcccg gcgataaggt catatacaac
     9781 aacagaggca aggcggtcat tctcgccgtc atcggcaaaa agagcatcaa agagggcgtc
     9841 agaatcagcg ccgctcacat cgattctccc agaattgact taaagcccaa tccgttgtat
     9901 gaagacagcg agcttgcgct tttcaaaacc cactattacg gcggaatcaa aaaatatcag
     9961 tggaccgcaa taccactttc tctgcacggc gtagtcatta aggcaaacgg agaaaaggtt
    10021 gagataacaa taggcgaggc agagggcgag ccaaagtttg taataacaga ccttctgccc
    10081 catcttggtc aggctcagtc ccagaaaaaa ctgggcgagg ctataaaggg tgaggatttg
    10141 aacattgt
//
LOCUS       DAONVQ010000069        10135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5084_length_10135_cov_4.233433, whole genome shotgun sequence.
ACCESSION   DAONVQ010000069 DAONVQ010000000
VERSION     DAONVQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10135
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5084_length_10135_cov_4.233433"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLD98_05570"
     CDS             <1..228
                     /locus_tag="PLD98_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064885.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HQA05753.1"
                     /translation="MGGNCDVQRYKGLGEMDPIQLWETTMDPARRTMLRVSLEDASQA
                     DETFSILMGDKVEPRREFIEKNAKYVQNLDI"
     gene            341..2872
                     /gene="gyrA"
                     /locus_tag="PLD98_05575"
     CDS             341..2872
                     /gene="gyrA"
                     /locus_tag="PLD98_05575"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQA05754.1"
                     /translation="MDNENIVTTSGGGKIIDVEINKEMKKSFLDYSMSVIMSRALPDV
                     RDGFKPVHRRILYTMYENGLTPDKPYRKCADTVGSVLGRYHPHGDASVYDAMVRLAQN
                     FSMRYPLVDGHGNFGSIDGDPPAAYRYTESRMSKIALEMLTDIDKETVEFRPNYDDRL
                     KEPVVLPSRFPNLLVNGSMGIAVGMATNIPPHNLTEVVDGMCCLIDNPDTDLDELMEH
                     IKGPDFPTAGIIMGRSGIRAAYGTGKGKLIVRARAEIEEGKNGRYRIVVTELPYQVNK
                     ARLIESIAEHVKEKKIEGIADINDYSSRNGMQIVIDLKKDANPQVVLNQLYTFTQMQV
                     TFGVIMLALVDGVPKILTLKDILTQYIDYQCDIIRRRTEFDLRKAKERAHIVEALLKA
                     LDFIDEVIAILRASKSIPEGKTNLMERFGFDEIQANHIVQMRLGQLTGLEREKLDEEM
                     ATLKAKIIEYTEILGSHERVLAIVKEEALEISKKYGDERRTEIVNVSGEVDVEDLIPE
                     ETCVLTLTTMGYIKRQTVDTYKLQRRGGRGIIGLTRREEDVAETMFTCSTHDHIMLFT
                     TKGRTYRLKGYEIPEGSRTSKGINIVNLLPLESEEKVSAMIRVAKEDESKYICMVTKK
                     GVIKRSELEEYRNVRRKGVIAINLDEGDELAYVLLTSGEDNLLVATRQGMCIGFKETD
                     ARAIGRTGRGVKAITLKEGDEVVGMTLLREGGKILTVSEKGYGRRSLSEDYRIQTRGG
                     LGVINYKTDRFGLVCAVREVKDDEDVILISAEGIIIRIPASEISIFSRPSKGVRVMRV
                     GENDKVATITTAEHSDDENEVDTLPADDGEEDTTPEEEIISEKTE"
     gene            3093..4250
                     /locus_tag="PLD98_05580"
     CDS             3093..4250
                     /locus_tag="PLD98_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HQA05755.1"
                     /translation="MERKTVVYRQKRGQVPLKKKQIFLVSVAVCIICALTVSGYSFVR
                     LSGINFVDPNSPGNTDFYDDFDEDFGEDEHFESMLGVSGARSLKEFLKKWSTNGGQHL
                     RSKKVLNILLLGLDNRDGDAISGNSDAIILVSLNKETKKITLVSLFRDSYTYINTPNG
                     ERYAKINAAYPYGGPECLIETIENDYKIDIDYYFAVAFKSFERVIDMIGGINVTVKEH
                     EAKYIRNKAEYNCKDMPYGENVLLNGKQALLFARIRGSDADGDISRTRRQRDVIAALI
                     KKSSQVSTSQITDLVKELANNVVTNCPATEIIRLATQGIVKKWYSYEIVDMAVPEEKD
                     RMAYNGDAWIWVVDYPAAAQKLQLALYGKTNIELTENRVTAMDVVKKYKVN"
     gene            complement(4354..4893)
                     /locus_tag="PLD98_05585"
     CDS             complement(4354..4893)
                     /locus_tag="PLD98_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQA05756.1"
                     /translation="MKFCDNCAARVSDDAVFCANCGMSLTSDSAPSQNNTQGAPQSSA
                     SPAENTSYNTYGDYYQQPYIPTDPFDHTHEFSPEEISENKVFAACVYILGVMGIIIGL
                     LAANSSKYVRFHVRQALKIYLLAMVSMILGLVFFWLIIPIIAASVFVIILFVVMIIGF
                     IDAWKGRAKELPIIRNFLK"
     assembly_gap    5224..5323
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5571..5843
                     /locus_tag="PLD98_05590"
     CDS             5571..5843
                     /locus_tag="PLD98_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05757.1"
                     /translation="MAEFLEAVMVICFGVSWPVSIAKSLKAKTAKGKSIVFLCFIIVG
                     YAAGIASKLISGRITYVFFFYCLNLLMVSADALIWVRNRRLDRGRE"
     gene            5909..6952
                     /gene="uxuA"
                     /locus_tag="PLD98_05595"
     CDS             5909..6952
                     /gene="uxuA"
                     /locus_tag="PLD98_05595"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HQA05758.1"
                     /translation="MKMTFRWYGKNDPVSLSHIRQIPCMTGIVSAVYDVPVGEVWPEE
                     SINAIKSAANSHGLEFEVVESVPVHENIKLGTPDSGRLIDNYCENVRRLGKAGVKVIC
                     YNFMPVFDWLRSDLETQLPDGSNALSYRHSRVLEMNPAESELSLPGWDESYTKDELKG
                     LLKKYEDLDEEGLWRNVKRFLEAVIPVAEESGVKMAIHPDDPPWSIFGLPRIITRMEN
                     LKRFLKLVDSEANGLTFCIGSLGAGRFNDLPAMIREIGGSRIHFAHLRNIKLTGEMDF
                     EESAHPTECGSLDMYEIMKALYDSGFDGYIRPDHGRMIWGEKGRYGYGLYDRALGATY
                     LVGLWEAISRAGK"
     gene            complement(7029..7484)
                     /locus_tag="PLD98_05600"
     CDS             complement(7029..7484)
                     /locus_tag="PLD98_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQA05759.1"
                     /translation="MPKFELTNMIMIKDPKTGLVAAIDRIKSPLGLSLPGGHIEDDES
                     FYDSAVREAYEETGLKVKNLKHCGVIHWLNTDTKDRYIVFLYKTSEFEGELKEFSDEG
                     KNKWVSVEELLSCKAETRIPEYMPMFLNDDLNEAFGPWRENEDFSIYYK"
     gene            7601..8590
                     /locus_tag="PLD98_05605"
     CDS             7601..8590
                     /locus_tag="PLD98_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium glycoprotein BclB-related protein"
                     /protein_id="HQA05760.1"
                     /translation="MTEYLQLIRAGGGFIEDTATLGFDSVAESSENISYDSGTGIITI
                     NRIGVYDIKWSVATQSAGNVPSIVFAVQTQNSGTFQGSSQVKTGEISGSATVVVDDAN
                     LPLSLWIENQSGQRVYLADYLPVKASLTIKMVEGIGATGATGITGATGPEGPPGVGAI
                     IPFSSGGAITLTTLVGGLVGAPSFVGFGSNFLGIDVLGTTINLEGDILGTALNFAFMV
                     PRDGVITRLDALFSATLGTVVGLEPYFVHAALYRSPDPANNEFTQIPESDIQLTPGLS
                     VLITLGEVLSGSVDLNVPVSRGDRLLLVFYLQPPTAGLAGTVTGYASAGLLIE"
     gene            complement(8834..9913)
                     /locus_tag="PLD98_05610"
     CDS             complement(8834..9913)
                     /locus_tag="PLD98_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HQA05761.1"
                     /translation="MFNGLGMNMGNLSRLSNAQTRSISPENFTGEKGKGGMSTDGPAL
                     KNAQRAGLGQGWKISPYIIIQPGETAVLADIKGMGAIQHIWMTLAGYYRFAILRMYWD
                     DSEKPSVECPVGDFFCDPQPGDFRQLTSLAVCVNPGSAFNCYWEMPFRKRAYITIENI
                     SDKEMTLFYQIDYCLTEVPDDCAYFHAQFRRVNPLPYKEVYTILDNVRGKGHYVGTYM
                     FWGVNNNGWWGEGEIKFYLDGDKEFPTICGTGTEDYFCGSYSFAVNNQYAEYCTPYAG
                     LHKVIKPDGANITQQRFSMYRWHIADPIRFEKDIKVTIQALGWRKGGAYLPLQDDISS
                     VAFWYQTLPVPEFPKLPDRDYLEII"
BASE COUNT         2591 a         2460 c         2650 g         2334 t
ORIGIN      
        1 atgggcggca actgcgatgt tcagcgatac aagggtctcg gcgagatgga ccccatccag
       61 ctttgggaaa ccaccatgga cccggcaagg agaaccatgc tcagagtatc tttagaggac
      121 gcgagccaag cggatgagac cttctcaata ctcatgggcg acaaggtcga gccaaggcgc
      181 gagttcatcg aaaagaacgc caagtatgtt caaaaccttg acatttaatt gttcaaaact
      241 cagcttgttc aaaatattgt tcaatgctgt ctaaaactta aatgtcgaaa acttctttca
      301 aattcggtga gaaactctca cagaaaggca cggtcactta atggataacg agaacattgt
      361 taccacatcc ggcggcggta agattattga tgttgaaata aacaaggaaa tgaaaaaatc
      421 cttccttgac tattccatgt cggtaattat gtcaagggca ctgcctgatg taagggacgg
      481 ctttaagcct gttcacagaa gaatacttta tacaatgtac gaaaacgggc tgactcccga
      541 caagccctac agaaagtgcg ccgacacggt cggttccgtt ctgggcagat accaccccca
      601 cggcgacgcg tcggtctatg acgctatggt gcgacttgct cagaacttct caatgaggta
      661 cccccttgtt gacggacacg gcaacttcgg ctctattgac ggcgacccgc ccgcagccta
      721 caggtacacc gagtccagaa tgagcaaaat cgcccttgag atgctgaccg acatcgacaa
      781 ggaaacggtt gagttcagac ccaactacga cgaccgctta aaggagccgg ttgttctgcc
      841 ttcacgcttc ccgaaccttc ttgtcaacgg ctccatgggc attgccgtcg gcatggctac
      901 gaatattccg ccccacaacc ttaccgaggt tgtggacggc atgtgctgcc ttattgacaa
      961 ccccgacact gaccttgacg agctgatgga gcatatcaag ggtcccgact tccccacagc
     1021 gggcataatt atgggacgct caggcatcag agccgcttac ggcacaggca agggcaagct
     1081 gattgtaagg gcaagggcag agattgaaga aggcaagaac ggcaggtaca gaatagtcgt
     1141 caccgagctg ccctatcagg taaacaaggc aaggcttatc gagagcattg ccgagcatgt
     1201 aaaggaaaag aaaatagaag gcattgccga catcaacgac tactcctcca gaaacggcat
     1261 gcagattgta atagacctta aaaaggacgc gaatccccag gtcgtcttaa atcagctcta
     1321 cacatttacc cagatgcagg taaccttcgg cgtaattatg ctggctctgg tggacggagt
     1381 gccgaaaatc ctgaccttaa aggatattct gacccagtac atcgactatc agtgcgatat
     1441 cataaggcgc cgcacagagt tcgatttaag aaaggccaag gaaagagccc acatagtgga
     1501 ggcgcttctg aaggccctcg acttcattga cgaggttatc gccatactca gagcctccaa
     1561 gagcataccc gagggcaaaa cgaaccttat ggaaaggttc ggctttgatg agattcaggc
     1621 aaaccacatc gtccagatgc gtctcggaca gctcacaggt cttgagaggg aaaagctgga
     1681 cgaggagatg gctaccctca aggcaaagat tatagagtac accgagattt taggctcgca
     1741 tgagagagtc ctcgccatag tcaaggaaga ggctctggaa atcagcaaga agtacggcga
     1801 cgagagaaga accgagattg taaatgtcag cggcgaggtt gatgtggagg accttatccc
     1861 cgaggaaacc tgcgttctga cacttacaac catgggctac atcaagcgtc agaccgtgga
     1921 cacatataag ctgcaaagga gaggcggcag gggcataatc ggtcttaccc gccgcgagga
     1981 ggatgtggcc gagaccatgt tcacctgctc cacccatgac catataatgc tctttaccac
     2041 caagggcagg acttacaggc ttaaaggcta cgagattccg gagggcagcc gcaccagcaa
     2101 gggcattaac atcgtcaacc tcctgcccct tgagtccgag gaaaaggttt ccgcgatgat
     2161 tagagtcgcg aaagaggatg agagcaaata catctgcatg gtcacaaaga agggcgttat
     2221 aaagcgcagc gagcttgagg aatacaggaa tgtccgcaga aaaggcgtta tagccataaa
     2281 tcttgacgag ggcgacgagc ttgcctatgt gcttttgacc tcgggcgagg ataatctcct
     2341 tgtagccaca aggcagggca tgtgcatcgg ctttaaggag accgacgcaa gagccatcgg
     2401 cagaacgggc aggggagtca aggcaatcac ccttaaagag ggcgacgagg tagtgggcat
     2461 gacgctcctc agagaaggcg gcaagattct caccgtcagc gaaaagggct acggcagaag
     2521 aagcctttcc gaggactacc gcatccagac aagaggcggt ctgggcgtca taaactacaa
     2581 gaccgacagg ttcggacttg tctgcgccgt aagagaagta aaggacgatg aggatgtaat
     2641 ccttatctcc gcagagggca taataatccg tatccccgca agcgaaatca gcatcttctc
     2701 ccgtccgtca aagggcgtaa gggtcatgag agtaggcgag aacgacaagg tggcaaccat
     2761 caccaccgcc gagcactccg acgacgagaa cgaagttgat actttgcccg ccgatgacgg
     2821 ggaggaggac acaactcccg aagaggagat tatttccgaa aaaacagaat aaaatataaa
     2881 gcggggataa attccccgct ttttgctgga ataattcaca aaatatgcgc cgattagtgc
     2941 ttataattta aaaattaaca gcttgatttt tcacattgta gtgatatact gaaaaattgt
     3001 tgggcatttt atactttgag cccgaaacaa aagtcttttc atcccagtct atataaacga
     3061 atatttagcg ctttaagtca ggaagtgtat ttgtggaaag aaaaaccgta gtttacaggc
     3121 aaaagcgagg acaggtgccg ctgaaaaaga agcagatttt ccttgtatct gttgcggttt
     3181 gcatcatttg tgcgctgacc gtcagcggat attcctttgt ccgcctttcg ggaataaact
     3241 ttgtggaccc caactcaccg ggaaataccg atttctatga cgactttgac gaggatttcg
     3301 gcgaggacga gcattttgaa agcatgctgg gcgtgtcggg agcgaggagc cttaaggaat
     3361 tccttaaaaa gtggtccacc aacggcggac agcatttaag gagcaaaaag gtacttaaca
     3421 tcctgctttt gggacttgac aacagagacg gcgacgccat atcgggcaac tccgacgcta
     3481 taattctggt gtcgcttaat aaggaaacca aaaagattac ccttgtttcg cttttcaggg
     3541 acagctatac ctacatcaac actcccaacg gcgaaaggta cgcaaaaatc aacgccgcgt
     3601 acccttacgg cggtcccgag tgccttatcg aaaccatcga gaacgactac aagattgaca
     3661 ttgactatta ttttgccgtc gccttcaaat catttgagcg ggttatagac atgataggcg
     3721 gcatcaatgt aacggtaaaa gagcacgagg ctaagtatat aaggaataaa gccgagtaca
     3781 actgcaagga tatgccctac ggcgagaatg tgctcttaaa cggcaagcag gctctgctct
     3841 ttgccagaat cagaggcagc gacgccgacg gagacatcag ccgcaccaga aggcagaggg
     3901 atgttatagc cgcccttata aagaaaagct ctcaggtcag cacctctcag attaccgacc
     3961 ttgtaaaaga gctggcaaac aatgtggtga caaactgccc cgcaacggag attatccgcc
     4021 ttgccaccca gggcattgtt aagaagtggt acagctacga gatagttgac atggcggttc
     4081 ccgaggagaa ggacagaatg gcgtacaacg gcgacgcttg gatttgggtt gtggattatc
     4141 ccgcagcggc gcaaaaactt cagctcgccc tttacggcaa gaccaatatt gagcttaccg
     4201 aaaacagagt caccgccatg gatgtggtga agaagtataa agtgaattaa atatatgccg
     4261 caggcgatga gcttgcggca ttttttgatg agcataagaa aagccgcaaa gcaaacttgc
     4321 ggctttaatt ttgctgagaa taagtttaaa acattatttc aaaaagtttc ttataatcgg
     4381 cagttccttt gccctgccct tccaagcgtc gataaagcca attatcataa ccacaaagag
     4441 gattattaca aatacggaag cggcaattat agggataatc agccagaaga atacgagacc
     4501 cagaatcatg ctgaccatgg ccagaaggta aatcttgagc gcctgacgga catggaagcg
     4561 gacatatttt gaggaatttg ccgcaagcag tccgatgatg attcccataa cgccgagaat
     4621 ataaacgcag gcggcgaaaa ccttgttttc ggagatttcc tcggggctga actcatgggt
     4681 atggtcaaag gggtcggtgg gaatataggg ctgctgataa tagtcgccgt aagtgttgta
     4741 gcttgtgttt tcagcgggcg aggctgagct ctggggcgcg ccctgcgtat tgttctgaga
     4801 aggcgcgctg tcggaggtaa ggctcattcc gcagttggcg cagaatacag cgtcgtcgct
     4861 gactcttgcc gcgcagttat cacaaaactt cataaaaaca ccccttacat ctttgatagc
     4921 cttattaaag catatttttt cccgattgta aatatacgca tccaacattt ttgacaaaat
     4981 ttagagaaaa tttaataatt ccctgtctat tttgcataaa gcttttgaat tttcccgccc
     5041 cagtccgtga ttcccgccca ccataggcgg gagggagagg gcggagagtc cgcccccgcg
     5101 ttttattgag aaccgcccgc tgagcggagt gcgccgccga actgcgggag agccagccgc
     5161 cgcaaagaat gcgccgcccg ccgaactgtg gggagagcca gccgccgcaa aggatatgcc
     5221 gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgcgcc gcccgccgaa
     5341 ctgtggggag agccagccgc cgcaaaggat atgccgcccg ggaggggaga gtcagcgctc
     5401 ctctgatggg aagttcccac ggcggaagtg tcacacccct taaaattagg ggagcggtga
     5461 cccgctgctt taacctgtta agctcaaaca aaatacaatt atatgctgcc tttttacttc
     5521 caccttttct cagattgtga tatactgcat acatagaaag gactggtttt gtggcggagt
     5581 ttttggaagc tgttatggtt atctgctttg gcgtttcgtg gcccgtttca atcgccaagt
     5641 ctttaaaggc gaagacggcc aagggcaaga gcattgtttt tctctgcttt atcatcgtgg
     5701 gttacgccgc gggtatcgcg tcaaagctga tttccggcag aatcacttat gttttctttt
     5761 tttattgcct taatctcctt atggtctcgg ccgacgctct tatctgggtc agaaaccgca
     5821 ggctggacag gggcagagag taggctttac tttttgtatt aaatcgttta aactgatatt
     5881 tggaatttct tgaaaggaag ataaaagtat gaagatgacc ttcaggtggt acggcaagaa
     5941 cgaccccgtg tcgctttccc acataaggca gataccctgc atgacgggca tagtcagcgc
     6001 cgtttatgat gtccccgtgg gcgaggtctg gcccgaggag agcataaacg cgattaagag
     6061 cgcggcgaac agccacggtc ttgagttcga ggttgtggag tccgttcccg tacacgaaaa
     6121 tattaaactg ggcacccccg acagcggcag acttatcgac aactactgcg aaaatgtcag
     6181 gaggcttggc aaagcgggag ttaaggtaat ctgctacaac ttcatgcccg ttttcgactg
     6241 gctgagaagc gaccttgaaa ctcagctccc cgacggctcc aacgctcttt cttacaggca
     6301 ctccagagtt ctcgagatga acccagcgga gagcgagctg tcgctgcccg gctgggacga
     6361 gagctacaca aaggacgagc ttaaagggct tttgaaaaag tacgaggatt tggacgagga
     6421 ggggctttgg aggaatgtaa agcgattcct tgaggcggtc attcccgttg ccgaggagag
     6481 cggcgtcaag atggccattc accccgacga cccgccgtgg tccattttcg gtttgccgag
     6541 gattatcaca aggatggaga atttaaagag attcttaaag cttgtcgact ccgaggcaaa
     6601 cggccttacc ttctgcatag gctcactggg cgcgggcaga ttcaacgacc tccccgccat
     6661 gattcgtgaa atcggcggca gcaggattca ctttgcccac ctcagaaaca taaagctcac
     6721 gggcgagatg gactttgagg agtccgccca tcccaccgag tgcggctccc ttgatatgta
     6781 cgagattatg aaggctcttt acgacagcgg ctttgacggc tacatacgcc ccgaccacgg
     6841 cagaatgatt tggggcgaaa agggccgcta cggctacgga ctttacgaca gagccctggg
     6901 cgccacatac cttgtgggtc tttgggaggc aatcagcagg gcaggaaaat aaatacaaaa
     6961 atcaaaggca ggttttgcgc ctgccttttt attttcgaaa tccaggagcg gaaatatccg
     7021 ctccgttttt atttgtagta aatactgaaa tcctcgtttt cccgccaggg tccaaaggct
     7081 tcgttcaggt catcgtttaa aaacatcggc atatattcgg ggattcttgt ctccgccttg
     7141 cagcttagga gttcttcaac gcttacccac ttgttttttc cctcgtcgga gaattcttta
     7201 agctcaccct cgaactcgct tgtcttataa aggaaaacaa tatacctgtc ttttgtgtct
     7261 gtattcagcc agtgtatgac gccgcagtgc tttaagtttt taactttcag ccccgtttcc
     7321 tcgtatgctt cccttacggc gctgtcataa aagctctcgt cgtcctctat atgtcccccg
     7381 gggagagaaa gccccagggg gctttttatt ctgtctatcg ccgcgacaag tcccgttttc
     7441 gggtctttaa tcattatcat gttcgtaagc tcgaatttcg gcatgctgtc accctttctt
     7501 ttgccccata attataccct aatcgggaca aattgttaac ctatttcggc gcttttcata
     7561 tgatgtcttg accccattct gaaaggaagg aatttaatat atgaccgaat atttacagct
     7621 tatcagagca ggcggaggat ttattgaaga taccgccact cttggttttg atagtgtagc
     7681 agaatcatca gaaaatatat cctacgacag tggtacagga ataatcacca taaaccgcat
     7741 aggagtttac gacattaagt ggtcggtggc aacccagtcc gcgggcaatg tcccgagcat
     7801 agtcttcgct gttcagaccc agaattcggg cacattccag ggcagctcac aggttaagac
     7861 cggtgaaatt tcaggctccg ccaccgttgt tgttgacgac gccaatttgc ccttgagttt
     7921 gtggatcgaa aatcagagcg gacaaagggt atatctcgcc gattacctgc ccgtaaaggc
     7981 aagtctcacc attaagatgg ttgagggcat cggcgctacg ggcgcgacgg gaataacggg
     8041 cgccacgggt cctgagggtc ctcccggcgt tggagccatt atacccttct cgtcgggcgg
     8101 agcgattacg ctcaccactc ttgtgggcgg acttgtggga gcccccagct ttgtgggctt
     8161 cggctccaac ttcttaggaa ttgatgttct cggcaccaca ataaaccttg agggcgacat
     8221 tctcggcacg gctcttaact tcgccttcat ggtgcccaga gacggcgtga ttacccgcct
     8281 tgacgctttg ttcagcgcga cactcggcac cgtggtgggt cttgaaccat actttgttca
     8341 tgccgctctt taccgctcgc ccgaccccgc caacaacgag tttacccaga ttcccgaaag
     8401 cgacatacag ctcaccccgg gactttcggt cttgatcacc ctcggagaag ttttaagcgg
     8461 ctctgtggac cttaatgtac ccgtaagccg cggcgacaga ctgctgttgg ttttctatct
     8521 ccagcccccc accgcgggtc tcgcaggcac ggttacggga tatgccagcg caggtctttt
     8581 gatagagtaa aactttccca atataaagcg gctgctttaa ggcagccgct gaagtttttt
     8641 aggcagagct cggacaactc gtgaccgaga attatttttc ttaaatgata tattggcaaa
     8701 ccgcggcgac aggctgatgg ttgtatttta tcttgaaggc attacggacc attgggagcc
     8761 tcggataagc ggctatggat tgagcagtcg cttcagcata aaaatttgcc cccgctttcc
     8821 gcaggggcag aatttatatt atctcaagat aatctctgtc ggggagcttg gggaattcgg
     8881 gcacggggag ggtctgatac cagaacgcga cggaggaaat atcgtcctga agggggagat
     8941 acgctccgcc ctttctccag ccaagagcct gaatggtgac cttaatatcc ttttcaaagc
     9001 ggatggggtc ggctatgtgc cagcggtaca tggagaacct ctgctgggta atgttcgctc
     9061 cgtcgggctt tatgaccttg tgcagacccg cgtagggtgt gcagtactcg gcgtactggt
     9121 tgttcaccgc gaaggagtag gagccgcaga aatagtcctc tgtgcctgtg ccgcagatgg
     9181 tggggaattc cttgtcgccg tcaaggtaga acttgatttc gccctcgccc caccagccgt
     9241 tgttgttgac gccccagaac atataggtgc cgacataatg acccttgccc cgcacattgt
     9301 caaggatggt gtaaacctcc ttgtaaggca gggggtttac cctgcggaac tgagcgtgga
     9361 agtatgcgca gtcgtcgggc acctcggtta gacagtagtc tatctgatag aaaagggtca
     9421 tttccttgtc ggatatattc tcaatggtga tgtaggctct ttttctgaag ggcatttccc
     9481 agtagcagtt aaacgcgctg ccgggattta cgcagaccgc gagggaagtc aattgcctga
     9541 aatcccccgg ctgcgggtcg cagaagaagt ctccgacggg gcattctacg ctgggctttt
     9601 cgctgtcgtc ccagtacatt ctgagaatgg caaagcggta gtagcccgca agggtcatcc
     9661 agatgtgctg aatggcgccc atgcctttta tgtcggcgag aactgccgtc tcgcccggct
     9721 gaattattat gtagggggag attttccagc cctgaccgag ccccgcccgc tgagcgtttt
     9781 taagcgcggg accgtcggtg ctcatgccgc ccttgccctt ttcgcccgta aagttttcgg
     9841 ggctgatgga ccttgtctga gcgttggaga gtcttgaaag gttgcccatg ttcataccca
     9901 aaccgttaaa cataatttca ccctttattg tttagttatg gataggataa cacagccccc
     9961 gaccgttttc aatatgccaa aaaggaaaaa gggcatagcg cctatggggg aggtgaccct
    10021 gcgtgggcgt gtgccgcagc ctgcggggga gggggagcgg tcatccctgc gactgacggg
    10081 gtgcgggcgg ggggaaagtt acccctgcgg gcggtggggt gggattgctt tcctc
//
LOCUS       DAONVQ010000070        10063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5144_length_10063_cov_4.376799, whole genome shotgun sequence.
ACCESSION   DAONVQ010000070 DAONVQ010000000
VERSION     DAONVQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5144_length_10063_cov_4.376799"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLD98_05615"
     CDS             <1..280
                     /locus_tag="PLD98_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQA05762.1"
                     /translation="LNVTTANTLKSLLRSNVKNYYGDSRFKGLELCAKTGTAEVGGEK
                     SPHAWFAGFSQREDFPYAFVCIVENGGSGSGTAAPIISRVLREMDKTY"
     gene            298..2676
                     /locus_tag="PLD98_05620"
     CDS             298..2676
                     /locus_tag="PLD98_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HQA05763.1"
                     /translation="MSEKLHPRLIYKTDPQPIDSCVIERGNVRITVLTDRLFRIETKP
                     KKGSFIDEATQIVWFRKLDTPVFTLKEKKKKLQISTTALTLFVNTKNGKASVVFKNTT
                     KKIPCENKDNLMGTARTLDMKNGKVKLGYGIMSKTGVSVIEDDSLILNAEGMPQPRPH
                     KGTDKYIFAYGKDFRAALRDYFRLTGRPPLIPRFAFGNWWSRYYAYTQDEYLELMNRF
                     EKENIPLAVATIDMDWHWVDVKKRFKAKREKLSVWGPGWTGYSWNTELFPDHVAMLKE
                     LHDKGLKVTMNLHPAFGVRFFEDRYEDMAKALDFDTSKKKTIKFDITSPKFVNAYFDV
                     LHKPLEDEGVDFWWIDWQQGKKSKIKGLDPLWALNHYHFADNEKNGKRPLILSRYAGT
                     GAHRYPLGFSGDAIVSWKSLNFQPYFTVNASNIGYTWWSHDIGGFALGKRSGELFLRW
                     LQFGVFSPITRLHAQKNDLAGKEPWKFDGLTKLAAKEYLRLRHKLVPYIYTMNYHTHI
                     GGRALCEPMYYAYPEEKEAYKVPNQYMFGTELIVCPITEKAKKKTKLASATAFIPEGR
                     WTDIFTGYIYEGKSFKKLYREKTDIPVLIKAGGIVPMSADEGNGCRLPKHLEILVSRG
                     NGSFEMFEDDGESLDYNLKNAKTRFIISEADEGYDFVIKAPMGDTSILPGGRKYSVKF
                     IDINEGKVSVEAGGNAIQCKVHMNPLRVELPNLPYDEDIRISVTQPVIAKNPDIRQHA
                     AELLSRIEGNNTIKAIKNRKLKKAKTEKKFRKKVKKVRFPRQVRKAILELYE"
     gene            complement(2727..4130)
                     /gene="murC"
                     /locus_tag="PLD98_05625"
     CDS             complement(2727..4130)
                     /gene="murC"
                     /locus_tag="PLD98_05625"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQA05764.1"
                     /translation="MSVLSDIDNVLSKVKRIHMVGIGGSGMFPLAEILHAKGYKLSGS
                     DNNESDILKRVRKLGIPVFLGHAAENLGDAEMVVQTAALLPNNPEIEEAKKRGIPIFS
                     RAELFGAVSRHFDNCIAVAGTHGKTTVTSMIVHILYLTGKDPSAVIGGKLPTIDSYGR
                     IGGSDIFVGEACEFADTFLHLSPACSIILNVDEDHLDYFKTMDNLKASFRKFADKATR
                     CIVYNGDDKNTLEVIDGLKKPLMISFGERPSNTWYAANINKVSNARHEFDIIFKGEKK
                     GTLKLAVPGYHNVLNALAAIAVCDYAGVSVQDSIKALESFMGAGRRFEILGRYGGVTI
                     ADDYAHHPQELKVTLQAAKAMDYKRVWAVFQPFTYSRTYMLMDDFADVLKIADKVVMT
                     EIMGSREINTYGVETSQLAAKIEGSVWFKTFEEVAKYVTDSAEEGDLIITLGCGDIYK
                     AAKMMCEIYESKGISKE"
     gene            4291..5187
                     /gene="dpsA"
                     /locus_tag="PLD98_05630"
     CDS             4291..5187
                     /gene="dpsA"
                     /locus_tag="PLD98_05630"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HQA05765.1"
                     /translation="MTTEKRILVLGGDSRQIYMSQYLRKNGARAVLFGFDNIKQEKPE
                     LEFDDLEFASGSLKDEISKSDIIILPLPVTRDNSYLNCAFSKKNVKLSEIVRHITKDH
                     IVFGGMIKDELFEKLSKKAAQVTDYFLREDLSVLNAIPTAEGVLGIIIDKLPITIHSS
                     ECAVTGFGRTGKAVGRSLRALGAKVTVSARSTDALANAISEGHQVCPLSELYKQAHKF
                     DVLINTIPSLVIDKKILQNLRRDCLIIEIASAPFGVDFAEADRLGINVIKAGSLPGKV
                     APKTAGKIISDTLINILKEGEK"
     gene            5184..5759
                     /locus_tag="PLD98_05635"
     CDS             5184..5759
                     /locus_tag="PLD98_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HQA05766.1"
                     /translation="MSNIRAGVAMCGSFCTFDEIFPVIEDLKNKGFDIVPIMSQNAYS
                     TDTRFGTAEEFRKRFVEISGKDIIHTIPQAEPIGPKKLLDILIIIPCTGNTLAKLANG
                     IADTAVTMAAKSHLRNQKPVLIGISTNDALANAAKNIGMLLNYKNVYFIPYRQDDYNG
                     KPTSMIADFSKTYDAAVAALAGKQLQPIITS"
     gene            complement(5802..6683)
                     /gene="ispE"
                     /locus_tag="PLD98_05640"
     CDS             complement(5802..6683)
                     /gene="ispE"
                     /locus_tag="PLD98_05640"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQA05767.1"
                     /translation="MKAVKYAAAKINLLLDVTEKLKNGYHSIFSVMQSIALYDKITVT
                     KNDAGKIRLSCSDKSVPTDEKNTAFKAAMLFFDEIHSDDRGIDIEIEKNIPQMAGLAG
                     GSADAAGVLLALNDLYKTNLSEETLIKLAGRVGADVPFCLKGGLMLALDTGLILAPLP
                     RLPEYHLVLVKPNEGVSTAEAYGAIDKKDWIRHPNKELALKALVDGDIHEFFSYSANV
                     FEQVIDAGARVEIKSVMRAHNCLYASMTGSGSAVFGVFMDKKDAEDCFSQLKNSSWQV
                     FLTKPVSKGVLSEGELE"
     gene            6897..7712
                     /locus_tag="PLD98_05645"
     CDS             6897..7712
                     /locus_tag="PLD98_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HQA05768.1"
                     /translation="MNCKNIMDLHVHTDNSFDGHHPAMFMCETAVSMGLRAVAFTDHC
                     EVDAYYTDSFDRSVRQSYLENAKARSAFMGQLLVLQGIELAQPHYDKELAEKIVSSYQ
                     YDIVIGSVHNLRDRRDFYYMESFNPDEIDSLVNEYLDELIGLAEWGGFDTLAHLTYPF
                     RYFYARSGITVDINEYKEKVDTILSLLVEKEKALEINTGGLRQPLNKLSPEFETIKRF
                     KDMGGKFITIGSDAHYAEHLASGFAEAKEAALAAGFKSETFFQSRSPFEIPIE"
     gene            complement(7761..7931)
                     /locus_tag="PLD98_05650"
     CDS             complement(7761..7931)
                     /locus_tag="PLD98_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQA05769.1"
                     /translation="MAYKISEECISCGACVDSCPVDAISEGDGIYVIDPETCIDCGAC
                     AGVCPVDAPQEA"
     gene            complement(8059..8709)
                     /locus_tag="PLD98_05655"
     CDS             complement(8059..8709)
                     /locus_tag="PLD98_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQA05770.1"
                     /translation="MKESLKNKIIEKLFSDYPSLQSLAADIHKAYELIENCYKNGGLV
                     MTCGNGGSAADSEHIVGELMKGFNLKRELSEEEKARFCGIEGGKEIADSLQGALPAIS
                     LVSQTSLISAFANDCSPDLVFAQQVFAYARTPFDVLIAMSTSGNSKNVVNAAKTAKAA
                     GIRCIGITGESGGLLKEYCDACIKLPKNTPYEVQELTLPLYHALCAMAEIEFFYRD"
     gene            complement(8719..9702)
                     /locus_tag="PLD98_05660"
     CDS             complement(8719..9702)
                     /locus_tag="PLD98_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQA05771.1"
                     /translation="MLLIGIDIGGTKCAVSLGAADMDKGIVEVLDKEAFPTENERGPF
                     KVIDEICEKIDLVLYRNSVSVSDIKGIGISCGGPLDSKKGIIKSPPNLKGWDDINIVK
                     IISDKYPVPVRLQNDANACALAEWRFGAARGSSNAVFLTFGTGMGAGLILDGRLYSGT
                     NDMAGEVGHIRLAEDGAEGYGKRGSFEGFCSGGGIARTACEMALKALEKGEVSSLLKS
                     KEDINERNLSAKNLAEAMKAGDKFALSVYELCAEKLGFGLSIIIDILNPEVIVIGSIY
                     ERNTEFFHNRVMKVIEKEALKISAEVCSIKPAELGDSIGDIAALAVAAGGF"
     gene            complement(9718..>10063)
                     /locus_tag="PLD98_05665"
     CDS             complement(9718..>10063)
                     /locus_tag="PLD98_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQA05772.1"
                     /translation="KETETESKPASTETTAKTHESSSSAIEKSSSGSSSTTKKSSSGG
                     SKGKLTKPININTAGKSELMRLKGIGEVKAQAIIDYRKTYGPFESKEDIMKVKGIGEK
                     TYENIKDWIVVN"
BASE COUNT         2632 a         2478 c         2357 g         2596 t
ORIGIN      
        1 ccttaatgtc accaccgcca atacgcttaa atcccttttg cgctcgaatg taaagaacta
       61 ttacggcgac tcaagattta agggtcttga gctttgcgca aagacgggca ccgcagaggt
      121 gggcggcgaa aagagccccc acgcatggtt cgctggtttt tcccagaggg aggatttccc
      181 ctatgccttt gtgtgcatag tggaaaacgg cggctccgga agcggcaccg ccgctcccat
      241 aatatcaagg gttttaagag aaatggataa aacatattga tgcagaggtg ttagagaatg
      301 agcgaaaaac ttcatccgag gctgatttac aaaaccgacc cgcagcccat tgactcatgc
      361 gtcattgaaa ggggcaatgt gcggattacc gtgctcaccg acaggctgtt cagaattgaa
      421 acaaagccga aaaaggggag cttcatcgat gaggcgactc agattgtctg gttcagaaag
      481 ctggataccc ctgtatttac cttaaaagaa aaaaagaaaa agctgcagat ttccacaacc
      541 gcgctgaccc tttttgtgaa caccaaaaac ggcaaggcaa gcgtggtttt caagaacacc
      601 actaagaaaa tcccctgcga aaacaaggat aacctcatgg gcaccgcaag gacccttgac
      661 atgaaaaacg gcaaagtaaa gctgggctac ggaatcatga gcaagacggg cgtcagcgta
      721 attgaggacg acagccttat cttgaacgct gagggaatgc cccaacccag accccacaag
      781 ggcaccgata aatacatatt cgcatacggc aaagatttca gagccgctct gagggactac
      841 ttcagactta cgggcagacc gccgcttatc ccccgctttg ccttcggcaa ctggtggagc
      901 aggtactatg cctacactca ggacgaatat cttgagctta tgaaccgctt tgaaaaggag
      961 aacatccccc tcgctgtcgc caccatagac atggactggc actgggtgga tgttaagaaa
     1021 aggttcaagg ctaaaagaga gaagctttcc gtctggggtc ccggctggac gggatacagc
     1081 tggaacacgg agcttttccc cgaccatgtc gcaatgctca aagagctgca tgacaagggt
     1141 ctgaaagtga cgatgaacct tcatcccgcc tttggagtgc gcttctttga ggacagatac
     1201 gaggacatgg caaaggctct ggactttgac acctcgaaaa agaagacaat taagtttgac
     1261 ataacctcgc ccaaattcgt aaacgcctac tttgatgtgc tgcacaagcc cttggaggat
     1321 gaaggcgtcg acttctggtg gattgactgg cagcagggca aaaagtccaa aatcaagggt
     1381 cttgacccgc tgtgggcttt gaaccactat cactttgccg ataatgaaaa gaacggcaaa
     1441 agaccgctta ttctttcaag atacgccggc accggcgctc accgctatcc cctgggcttc
     1501 tccggcgacg cgatagtaag ctggaagtcc cttaatttcc agccctactt cacagtaaac
     1561 gcctccaaca taggctatac atggtggagc cacgacatcg gtggcttcgc tctgggcaaa
     1621 aggagcggtg agctgttcct gagatggctg cagttcggcg tgttctcccc cattacccgc
     1681 ctgcacgctc aaaagaacga ccttgctggc aaggagccgt ggaaatttga cggtcttacc
     1741 aaacttgccg ctaaggagta tttgcgtctt aggcacaagc tggttcccta catatatacg
     1801 atgaattatc atacccatat cggcggcagg gctttgtgcg agccgatgta ctacgcctat
     1861 cctgaggaga aggaagcata caaggttccg aatcagtaca tgttcggcac cgagcttatt
     1921 gtctgcccca taacggaaaa ggctaagaaa aagacaaagc tggcgtctgc cacagcattt
     1981 attcccgaag gacgctggac ggatattttc actggctata tctatgaggg caagagcttt
     2041 aaaaagctct acagagaaaa gaccgatatt cccgtactta tcaaagcggg cggcattgtg
     2101 cccatgtccg cggacgaggg caacggctgc agactgccaa agcaccttga aatcttagtc
     2161 agccgcggaa acggcagctt tgagatgttt gaggacgacg gcgagagcct tgactacaat
     2221 ctgaaaaacg ccaagacaag gttcataatc tctgaggccg acgagggcta tgactttgta
     2281 atcaaggctc ccatgggcga cacatcgata cttcccggcg gcagaaagta cagcgtaaag
     2341 tttattgaca taaacgaagg aaaggtcagc gttgaagcgg gcggaaacgc cattcagtgc
     2401 aaagttcata tgaatcctct cagagtcgag ctgccgaacc ttccctatga tgaggacatc
     2461 aggataagcg taacccagcc cgtcatagca aagaatcctg atatcaggca gcatgccgcg
     2521 gagcttctgt caaggattga gggcaacaac acaataaagg ccattaagaa cagaaagctc
     2581 aaaaaggcta agacggaaaa gaaattccga aagaaggtaa agaaagtccg cttcccccgc
     2641 caggtcagaa aggcaatact cgagctatat gaataaagaa aaatccctga gtaaatctca
     2701 gggattttaa tttcttacgg gaacttttat tctttggata tacctttgct ctcgtaaatc
     2761 tcgcacatca tttttgcggc tttgtagata tcgccgcagc cgagggttat tataaggtcg
     2821 ccctcctctg cgctgtcggt aacatatttt gctacctcct caaaggtctt aaaccacacg
     2881 ctgccctcta ttttcgcggc aagctgggag gtttcaaccc cgtaggtgtt tatctccctt
     2941 gagcccatga tttcggtcat aacgaccttg tcggctattt taagaacatc cgcgaaatcg
     3001 tccatgagca tataggttct ggagtaggta aagggctgga aaaccgccca tacccttttg
     3061 taatccatag ccttcgcagc ctggagggtt actttaagct cctggggatg gtgggcgtag
     3121 tcgtcggcta ttgtgacgcc gccgtatctg cccaatattt caaagcgtct gcccgcgccc
     3181 ataaagctct caagagcctt aatgctgtct tggacgctga ccccggcgta atcgcagacg
     3241 gcaatcgccg caagggcgtt tagcacattg tgatagccgg gcaccgcaag ttttaaagtg
     3301 ccttttttct cgcccttaaa aataatgtca aattcatgcc ttgcatttga aaccttattt
     3361 atatttgcgg cataccatgt atttgagggg cgttccccga aacttatcat caaaggcttt
     3421 ttcagcccgt caataacttc aagcgtgttt ttgtcatcac cgttgtagac aatgcacctt
     3481 gttgccttgt cggcaaattt tctgaaggag gctttaagat tatccattgt tttgaagtag
     3541 tctaaatggt cctcgtcgac attgaggata atgctgcagg cgggagacag gtgcaggaag
     3601 gtatcggcaa actcgcatgc ctctcccacg aaaatatcgc tgccgcctat cctgccgtat
     3661 gaatctatgg taggcagctt gccgccgata accgcagagg ggtccttgcc tgtaaggtaa
     3721 aggatatgga ctatcattga ggttacggtg gttttgccgt gggtacccgc gaccgctatg
     3781 cagttatcaa aatgacggga aacggcgccg aaaagctccg cccttgaaaa tatggggatg
     3841 ccgcgttttt ttgcctcctc gatttcgggg ttgttgggca aaagagccgc ggtctgaacc
     3901 accatctcgg cgtcccccaa attctcggcg gcgtgaccta aaaatacggg aatccccagc
     3961 tttctgactc tttttaaaat atcactttcg ttattgtcgg agccggaaag cttatatccc
     4021 ttagcgtgaa gaatctcggc aagggggaac attcccgagc cgcctattcc caccatgtgt
     4081 attcttttaa cttttgaaag aacattatct atatcgctca aaactgacat ctccttaggg
     4141 cagtaaacaa ttcgcacagt tattattata cgctaatatt ttccttttta acagagaata
     4201 ttgaatttaa ttttatctct ttaacaatta accgcaactt tttcacacat atctcggact
     4261 aatcataaaa tgcaatcagg aggactgctt atgactacag aaaaaagaat tctcgtgctc
     4321 ggcggcgaca gccgtcaaat atatatgtcg caatacttac gaaaaaacgg cgcaagggca
     4381 gtgctctttg gctttgacaa tataaaacag gaaaagcccg aacttgaatt cgacgacctt
     4441 gagttcgcct cgggctctct taaagatgag atttcaaagt cagatataat catcttgcct
     4501 ttgcccgtta ccagagataa ttcttatctc aactgcgctt tttcaaagaa gaatgttaaa
     4561 ctctcggaaa tcgtaaggca tattaccaaa gaccacattg tttttggcgg aatgataaag
     4621 gatgaactgt ttgagaaact ctctaaaaaa gcggcgcagg ttaccgacta cttcctgcgt
     4681 gaggatttgt ctgttctgaa tgccattcct actgcggagg gcgtgttggg aattattatt
     4741 gataaactgc ccatcaccat tcactccagt gaatgcgccg ttacaggctt tggcagaaca
     4801 ggaaaagcag tgggcaggag tctcagagct cttggcgcaa aggtaacggt ctcggcaaga
     4861 agcaccgacg ctctcgcaaa cgccataagc gagggccatc aggtatgtcc tctttccgag
     4921 ctttacaaac aagcccataa gttcgatgtc cttataaaca ctataccctc tctggttata
     4981 gacaagaaga tacttcagaa tctgaggcga gattgtctta taatagagat tgcctccgcg
     5041 cctttcggcg ttgattttgc cgaggcagac aggctgggca taaatgttat taaggcaggc
     5101 tctctgcccg gaaaggttgc tcccaaaacg gcgggaaaga taataagcga tacccttatc
     5161 aatatactaa aggagggtga aaaatgagca acataagggc cggagttgcc atgtgcggtt
     5221 cgttctgcac ctttgatgaa attttccccg taatcgagga tttaaagaac aagggttttg
     5281 atatagtgcc tataatgagc cagaacgcct attcaaccga taccagattc ggcactgccg
     5341 aggagttccg caagagattc gtggaaatca gcggaaagga tataatacac accatccccc
     5401 aggcggagcc tataggtccc aaaaagctcc ttgacattct tattataatc ccctgcacag
     5461 gaaacaccct tgcaaaactg gcaaacggaa ttgcggacac agccgtaacg atggcagcaa
     5521 aatcgcacct gagaaatcaa aaacccgtgc tgatcggcat atccacaaac gatgccctgg
     5581 ctaacgcggc aaaaaacata ggaatgctcc tcaattacaa gaatgtttac tttatcccat
     5641 acaggcagga tgactacaac ggaaagccca cctccatgat tgcggatttc agcaaaacat
     5701 acgacgccgc tgttgccgcg ctggcaggaa agcagctgca gcctattatc acttcctaaa
     5761 aagcagaaaa taaaagcccc ttttataagg ggctttgttt tttactctaa ttctccctca
     5821 cttaaaacgc cctttgagac aggctttgta aggaatacct gccatgagct gttttttagc
     5881 tgactgaaac agtcttcggc gtccttttta tccatgaata ctccgaaaac cgccgagccg
     5941 ctgcccgtca ttgaggcata aaggcagttg tgggctcgca ttacgctctt tatctccact
     6001 cttgcgcccg cgtctatgac ctgctcgaaa acattggcgg aatatgagaa gaattcatgt
     6061 atatccccgt caacaagcgc tttgagggca agctccttat tcgggtgacg aatccaatcc
     6121 tttttgtcaa tggcgccgta agcctctgcg gtggagaccc cttcattggg ttttacaagc
     6181 acaagatggt attcggggag ccttgggagc ggggccagaa tcagacccgt atcaagggca
     6241 agcataagcc cgcctttaag gcagaagggc acatcggcgc cgactctgcc ggcaagttta
     6301 ataagggttt cctcgctgag atttgtttta tacaggtcat tgagagccaa aagcacaccc
     6361 gcagcgtccg cgctgccgcc ggccagaccc gccatctggg gtatgttctt ttctatttct
     6421 atgtcgattc ctctgtcatc ggagtgaatt tcgtcgaaaa agagcatggc agccttaaaa
     6481 gccgtatttt tttcatccgt cgggacagac ttgtctgagc agcttagtct gattttaccc
     6541 gcgtcgtttt ttgtgacggt gattttatcg taaagagcta tggactgcat aacggaaaaa
     6601 atgctgtggt agccgttttt cagcttttcg gtcacatcaa gaagcagatt aatcttcgcg
     6661 gcggcgtatt ttactgcctt cattctgtta ctctccttac acacccaaaa tccatgttgc
     6721 gttagctaat aaaacaatcg caggtttata catttttacc ataaaattcg acatcaattt
     6781 gcattttgtc gtaaaactct gagaacatta tattttaatt aattttttgt gtcaataaat
     6841 ctgcgcttat gctattattt acggtgagga caaagaggag cagcttaggt gattatatga
     6901 attgtaaaaa cattatggac cttcatgtgc acacagataa ctcttttgac ggacaccacc
     6961 ccgccatgtt catgtgcgaa acagcggtca gcatgggact gagagccgtc gcctttaccg
     7021 accactgcga agttgacgcg tattacaccg acagctttga ccgctcggtc aggcagtcat
     7081 accttgaaaa cgcaaaggca aggtcagctt ttatgggtca gcttttggtg ctgcagggca
     7141 ttgagcttgc ccagccccat tacgacaagg agcttgcgga aaagattgtt tcgtcttatc
     7201 agtacgacat tgtaatcggc tcggttcata acctcagaga ccgcagggat ttttattata
     7261 tggaaagctt taatcccgat gagattgatt cccttgtcaa cgaatatctt gatgagctta
     7321 tcggtcttgc cgagtggggc ggatttgaca ctctggcgca tctgacatat cccttcagat
     7381 atttttacgc ccgctccgga atcaccgttg atataaacga atacaaggaa aaggttgaca
     7441 caatactttc tctgcttgtg gagaaggaaa aggctctcga aattaatacg ggaggcttaa
     7501 ggcagccttt aaacaaactt tcccccgaat tcgaaaccat aaagaggttc aaagacatgg
     7561 gcggcaaatt cataaccata ggctccgacg cccattacgc ggagcacctt gcctccggat
     7621 ttgctgaggc taaggaggct gctctcgccg ctggctttaa gagcgaaaca tttttccaga
     7681 gccgcagccc atttgagatt ccgattgaat aaagacaaaa caataaaggg cggaaaaaac
     7741 cgccctctat ttttttcagt ttatgcctcc tgaggagcat ccacagggca tacacccgca
     7801 catgcaccgc agtcaataca ggtctcaggg tcgattacat atatgccatc gccttcggaa
     7861 attgcatcaa cagggcagct atctacgcaa gctccacagc taatacactc ttctgaaatc
     7921 ttatatgcca tctctgcacc ctccttttcc gttattaaac tgaatatgtt aaattaattc
     7981 agacagtgaa acaggtgttt tttcaccaaa ctttataata acacgactac tcgcaaaaaa
     8041 caatgctttt tgtaaatatt aatctctgta aaagaattca atctccgcca tggcgcaaag
     8101 ggcgtgatag aggggcagag taagttcctg aacctcataa ggcgtgtttt tggggagctt
     8161 aatgcaggcg tcgcagtatt cttttaaaag accgccgctc tcccccgtta tgcctatgca
     8221 gcgaattccc gcggccttcg ccgtctttgc ggcgttgacc acattcttcg agttgcccga
     8281 tgtgctcatg gcaataagca catcaaaggg ggttctggcg taggcgaaaa cctgctgagc
     8341 aaagacaagg tctggtgagc agtcgttggc aaaggcggaa attaaggagg tctggctcac
     8401 aagtgagatt gcggggagcg cgccctgaag ggagtcggca atttccttgc cgccctcaat
     8461 gccgcagaac ctcgctttct cctcctctga aagctcacgc tttaaattaa agcctttcat
     8521 cagctcgccc acaatatgct cgctgtcggc ggctgagccg ccgttgccgc aggtcattac
     8581 caaaccgccg tttttgtagc agttttctat aagctcatag gctttatgga tgtctgccgc
     8641 aagggactgc aaagagggat agtcggaaaa cagcttttct attattttgt ttttaaggct
     8701 ctctttcata tttcctcctt aaaagcctcc cgcggcgacc gctagggcgg caatatctcc
     8761 tatagagtcg cccagttcgg cgggttttat actgcaaacc tcggcagaga tttttaatgc
     8821 ctctttctca ataactttca tgactctatt atggaaaaac tctgtgttcc tctcataaat
     8881 actgccgatg acaatgacct cggggttgag aatgtcgatt ataatagaca gcccgaaccc
     8941 cagcttttct gcgcatagct catagacgga aagggcgaat ttatctcccg ccttcatagc
     9001 ctcggctaga ttcttcgcgc tgagatttct ctcgttaata tcctctttag attttaagag
     9061 gctgctgact tctccttttt caagagcctt cagtgccatt tcgcaggcgg tgcgggctat
     9121 tccgccgccg ctgcaaaatc cctcaaagga gccccttttg ccgtagccct cggctccgtc
     9181 ctcggcgagt cttatatgac ccacctcgcc cgccatgtcg ttggtgccgc tgtagagtct
     9241 gccgtcaagg ataagccccg ctcccatgcc tgtgccgaag gtcaggaata cggcgttgct
     9301 gctgcccctt gccgcgccga aacgccactc tgcaagggca caggcgttgg cgtcgttctg
     9361 gagcctgacg ggtacggggt atttgtctga gataatcttt acaatattaa tgtcgtccca
     9421 gccctttaaa ttgggcgggg attttattat gccttttttg ctgtcaaggg ggccgccgca
     9481 gctaattccg atgcccttta tatctgaaac ggacacgctg tttctatata ggacaaggtc
     9541 aatcttttcg catatctcgt cgataacctt aaagggacct ctttcgttct cggtgggaaa
     9601 ggcctctttg tccagcacct cgacaattcc tttgtccatg tccgctgcgc ccaaagaaac
     9661 ggcgcacttg gttccgccta tgtctatgcc tataaggagc atatcagatt cctttcgcta
     9721 atttacaact atccagtctt tgatgttttc ataggttttc tcgcctatgc cttttacttt
     9781 cattatgtct tccttgctct caaaggggcc gtatgttttt ctgtagtcaa tgatagcctg
     9841 agccttgact tcgccaatgc cttttaagcg catgagctcg cttttgcccg cggtgttgat
     9901 gttgatgggc ttggtaagct tccctttgga gccgccggag cttgactttt ttgttgttga
     9961 ggaacttcct gagcttgatt tctcaattgc tgatgagctg ctctcatgcg ttttggcggt
    10021 ggtctcggtg cttgcgggtt tgctttccgt ttcggtttct tta
//
LOCUS       DAONVQ010000071        10005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5188_length_10005_cov_4.164322, whole genome shotgun sequence.
ACCESSION   DAONVQ010000071 DAONVQ010000000
VERSION     DAONVQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5188_length_10005_cov_4.164322"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..497)
                     /gene="cas2"
                     /locus_tag="PLD98_05670"
     CDS             complement(207..497)
                     /gene="cas2"
                     /locus_tag="PLD98_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HQA05773.1"
                     /translation="MFVLITYDVNTQTASGRKRLNKLAKQCVNYGQRVQNSVFECVID
                     AAQFRKVKDQLEKIIDPQCDSLRFYYLGKNYKNKVEHIGAKPSYDPEAPIVL"
     gene            complement(532..1566)
                     /gene="cas1c"
                     /locus_tag="PLD98_05675"
     CDS             complement(532..1566)
                     /gene="cas1c"
                     /locus_tag="PLD98_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HQA05774.1"
                     /translation="MRKLLNTLYITTNDKYLSLDGENVVVSQDGKEVARYPLHRFEAI
                     VTCGYSGVSPALMGACAKRGISISFLSMSGKFLARVCGETCGNVVLRKTQYRYSDDTE
                     ISNHIAKSFLTGKLYNAKWVLERATRDHPMRLNVEELKQKSAQLSKCINNIQCAENLE
                     QLRGIEGEGAKAYFCVFDQLILNQKEDFSFDTRNRRPPLDNVNALLSFSYSLLLSTCI
                     SALESVGLDPYVGFLHRDRPGRASLALDLMEELRPALADKFVLSLINRKEINKDCFIQ
                     KENGAVEITDNAKKKILTCWQNKKSEEIKHPYLDIKIEWGVVPYAQALLLARYIRGDI
                     DGYPPFLWKS"
     gene            complement(1573..2235)
                     /gene="cas4"
                     /locus_tag="PLD98_05680"
     CDS             complement(1573..2235)
                     /gene="cas4"
                     /locus_tag="PLD98_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HQA05775.1"
                     /translation="MISHYDYLQLSGIQHFVFCRRQWALIFIENQWADNLRTVEGTLL
                     HEKAHDPTSDEKRKDIIIVRGMPIKSDKLRITGICDVVEFIADENGIHINNYEGKYKV
                     YPIEYKRGEPKENEADILQLVAQAICLEEMLCCSINEGAIYYGKTRRRQKVQITDSLR
                     NSTTRFFKEMHEYTQRQYTPKVKPTKSCNACSLKNICLPKLMKSKSVKDYIANHIADE
                     KA"
     gene            complement(2232..3095)
                     /gene="cas7c"
                     /locus_tag="PLD98_05685"
                     /pseudo
     CDS             complement(2232..3095)
                     /gene="cas7c"
                     /locus_tag="PLD98_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984820.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
     gene            complement(3120..5111)
                     /gene="cas8c"
                     /locus_tag="PLD98_05690"
     CDS             complement(3120..5111)
                     /gene="cas8c"
                     /locus_tag="PLD98_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HQA05776.1"
                     /translation="MDSFYYYYYTQRETHNEMGWAQKLAETYDNCLSEVGVFYQDKTP
                     LLPIYHTTQQAHIEILIDSDGNFLYGRSRVITDKGDATTIIPCTEDSALGTNDRAPHP
                     LFDKLNYLAGDYKKYANQDGKFDKYIKNLRLWCESPYKHPKIVSIMRYLEKGTLIKDL
                     IDDGILFTNENNLLIDKWTDPDPPPIFKVVNSQDKAFVRIAVIPSDGSDNADIYPWLD
                     KSLWNSFICYQDSLESDSDYCYATRNFVPISRLSPKKIRNPGDSAKLISSNDSSNFTY
                     RGRFAKPEQAFCIGRETTEKAHNALRWLISKQGTYHDSQVIVAWGTKNEEIPPICADS
                     LDLVAALYPSDEPVVFTNEKFANELNKAINGYKANLKEDNQAVVMGMDSATSGRSAIR
                     GRLSIFYYRELLILDLIERIEKWHKTCSWKLEYRKIKTGKDEKGKEKYEKFAFVGAPA
                     PKDIAEAAYGTNIKENHKKSIIERLLPCIIDGAKVPVDIMKNAARRASNPASFKEDWE
                     YRKTLSIACALIRKYYNDKSKNKEVFKMGLDFENNDRNYLFGRILAYIREIESKALYD
                     AKENRQTNAERLLNAFCRAPAKYCDILHKQLQPYLKRLGGKGFFLESEMMALISRLDT
                     EFNNTPLNETFLLGYSAQMNHFIEGSRTHKQNETETFEE"
     gene            complement(5039..6676)
                     /gene="cas3"
                     /locus_tag="PLD98_05695"
     CDS             complement(5039..6676)
                     /gene="cas3"
                     /locus_tag="PLD98_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HQA05777.1"
                     /translation="MISLAIISHHHNGLYDIISPDGKDVFEVRINPKREIFYDEVLKK
                     IAHDKEILSDDFDSSATEIENIFNKLAKLYPNDKASLVFSLGLIVRYLLSCVLDADRY
                     DSYLFENNEKVTNMTDDNFFAKLSDELENHIRKLDKSGSVNKERQKISDACLSFAKND
                     NGIYQLHVPTGGGKTLSSLRYAVNCAKMNGKDRIFYIAPYKSILEQNADVVRGISKYC
                     KEEILEHHSDIVSSDDRYKLLTERWSSKIVLTTMVQFLDTLFAGKSSAARRFHSLANS
                     VIIIDEVQSIPVNCIYMFNTAMNFLASVMNCSVVLCTATQPRLHLTKKYPLMLSNKRM
                     IEDTTEIFKRFKRTQIRDLTIEGVFSSERLAEFIAEKHKDNKRILAILNTKKAALKAF
                     LCLKENYPSFNVCYLSTGLCPAHRRKVLEKIRYEKEVICISTQLIEAGVDISFSIVIR
                     SLAGLDSIAQAAGRCNRNKELENGDVYIIRFKEENIDKLVDIREGQKAALNVLAAFEK
                     NLKDLTTIYYRLRQWTVFITITIHSAKLTMKWAGRKN"
     gene            complement(6673..6984)
                     /locus_tag="PLD98_05700"
     CDS             complement(6673..6984)
                     /locus_tag="PLD98_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HQA05778.1"
                     /translation="MQELSMNTYYARKTKDTRIQTLCEHSKNVEEFCKSFGAKIGVAK
                     TASLCGKLHDIGKATEEFQQYLLGESDKKEVRLIIQVQAVNWHTLFLLKRIHLKRLPL
                     K"
     gene            complement(7187..7273)
                     /locus_tag="PLD98_05705"
     tRNA            complement(7187..7273)
                     /locus_tag="PLD98_05705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7237..7239),aa:Leu,seq:taa)
     gene            7434..8948
                     /locus_tag="PLD98_05710"
     CDS             7434..8948
                     /locus_tag="PLD98_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05779.1"
                     /translation="MSESSLINRRQYSALYKILAVIVAAFGTGFMWRARGSHGFGGMF
                     GMFSVAAFMTLLILAIFGDRKKNIYEMIPLMVFATGVTAGGWGTLNRQMAGVLDTTSV
                     YSTLGESTYVNISPFSGMLIMLMLGFGWLPFFGFLLGRFFTDKRYKVKHLIIAALVFY
                     SVQYILKATLAHPILELFHPQAAELFAKGLAEQGLDTNQWLMYLKHFNGMSWAKKITG
                     GRNYFTSIEVVSKAIAAAAVMLYQRFGVKDKRGSVINFCTCAIMAVSITLADVIMLFD
                     VPGGIFEHIKKPQWLKYTWSYWEYFTGFLLGLGVMILCFIADKKLKDNWNLADDFPTI
                     SRKLRAAYNTLTTLFFTFGLALAMPAISRVYDQEYTLTGKKVFYSLEDYNAAIQTNPD
                     LRAVCFKNPIPIAVTYVVVLAALLAVYAYIVHKNMIKKGLDTPIDMSPVRFAGRAYIT
                     YFLIVAALYFFTNGAALLSPVKDSVTKTMLVSFAVVILGIIGLKLSDRQVKSKA"
     gene            complement(8983..>10005)
                     /locus_tag="PLD98_05715"
     CDS             complement(8983..>10005)
                     /locus_tag="PLD98_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HQA05780.1"
                     /translation="GYYGSGFSGLLTGLLLGHMAGSMGRSTWGSRGSGGFGGFDSSDG
                     FGGFGGFDFDLGDIFSSFFGGFGTGQRRANPNAPQRGSDIHTTLTISFEEAAKGCKKT
                     VNVDRIEVCDTCSGSRAAPGTHSQTCSECGGSGQVRIQQRTPFGMITTMRTCSKCGGS
                     GVFIPTPCNTCKGTGRIRRNVSIEVDVPAGIDDGQAISSRGNGNKGLNGGPAGDLIIN
                     IHVRPHPFFERDGYNVWCEITVTFVQAALGDELKVPTLDGKVKFKLPAGTQPGSVLKL
                     KGKGIPHLNGRGVGDQLLRINVEVPRNLTKRQKEILLQFNEEMGNKPLGEPEDDKKGF
                     FGKKKK"
BASE COUNT         2597 a         2231 c         2082 g         3095 t
ORIGIN      
        1 ttatttcaat ccacgctctc cgtgtggaga gcgtccgcaa aaatacacaa ctatcttacg
       61 cattagaaag tactaatcat actatgttca cgaaaaaaag attgatggta ataaattgtt
      121 aataaagaaa acaaaaaatt aatatgaaat ccgtgtcttt tttagtgcga gactaccgaa
      181 gtttttatgt gcatttatgg ttcgcactat aaaactattg gtgcttctgg gtcatatgaa
      241 ggttttgctc cgatgtgttc taccttattt ttgtaatttt ttccaagata atagaatctg
      301 aggctgtcgc attggggatc aataattttc tcaagctgat cctttacttt tcgaaactga
      361 gcggcgtcaa taacgcattc aaatacagag ttttggactc tttgtccgta attgacacat
      421 tgttttgcaa gtttgttcaa tcgttttctg ccgcttgcgg tttgagtatt cacatcataa
      481 gtaattaaaa caaacatagc attttcgtcc tctcatcagt aaactttaaa cttatgactt
      541 ccataaaaat ggtggatagc catctatgtc gccgcggata tatcgagcaa gcaaaagtgc
      601 ctgtgcataa ggcacaacgc cccattcaat ttttatatcg agataagggt gtttgatttc
      661 ttcagatttt ttattttgcc aacaagtaag aattttcttt tttgcgttat cggttatttc
      721 aacggcgcca ttttcttttt ggataaaaca gtctttattg atttctttgc ggttaataag
      781 tgaaaggaca aatttatccg caagggcagg gcgcaactct tccataagat ccaaagccaa
      841 cgaagctctt ccgggccgat cgcgatgcag aaaaccaaca tacggatcca gaccaacaga
      901 ttccagagca gaaatacagg tgcttaacaa aagagaataa ctaaatgata gcaaagcgtt
      961 aacattatca aggggaggtc gtctgtttct tgtatcaaac gaaaaatctt ctttctgatt
     1021 taaaatcagt tggtcaaata cgcaaaaata agccttagcg ccctcgccct caatacctct
     1081 aagctgttca agattttccg cacactgaat attgtttata catttgctta gctgtgcgga
     1141 tttttgtttc agctcttcaa cattcaggcg cattggatga tctcgggtcg ctcgttcaag
     1201 tacccatttt gcattataca gttttcccgt tagaaaactt ttggcaatat ggttgcttat
     1261 ttcggtatcg tcagaatacc tgtattgagt ttttcgcaaa actacattgc cgcaggtttc
     1321 tccgcaaact cttgcaagga attttccgct catcgacaaa aaggaaatcg agatgccccg
     1381 cttagcgcag gcgcccatta aagcggggct tactcccgaa taaccgcaag ttactattgc
     1441 ctcaaagcga tgaagaggat atcttgctac ttccttacca tcctgagaga caacaacatt
     1501 ctcgccatcc agagaaagat acttgtcatt cgttgtaata tacagagtgt tcaaaagctt
     1561 cctcatgaca actcaggcct tttcatctgc aatgtggtta gcaatatagt cttttacact
     1621 tttgcttttc ataagtttcg gcaagcatat gtttttcagt gagcacgcat tgcagctttt
     1681 tgtaggttta actttcggag tgtactgcct ttgggtatac tcgtgcattt ctttaaaaaa
     1741 tcgggttgta gaatttctta gcgaatcagt aatttggact ttctgccttc ttcttgtttt
     1801 gccgtagtaa atggctcctt cgtttataga acagcatagc atttcttcaa gacatattgc
     1861 ctgcgcaaca agttgaagaa tatcagcctc gttctctttt ggttcaccgc gcttgtattc
     1921 aatgggatat actttgtact ttccttcata attgtttata tgtatcccat tttcgtcagc
     1981 gataaactca accacatcgc aaataccggt tattctcagt ttgtccgatt tgattggcat
     2041 tcctctgaca attattatat cttttcgttt ttcatcggat gtagggtcgt gagctttttc
     2101 atgaagaaga gtaccttcaa ctgttcgcaa attgtctgcc cattgattct caataaaaat
     2161 cagagcccat tgtctacggc agaaaacgaa atgctgtatg cccgaaagct gcaggtaatc
     2221 ataatggctt atcataccag ctcatcgtaa atctcaggtt caagaccttc aaggtgttca
     2281 agctctatgc tgtaatcctc cagattgcgc ggggtatcgg ttttggcctt aactttaagc
     2341 gaacgatgta cctttgctga tgagtactgg ccgtttttgt ttttatgttt ccaccaatat
     2401 actttgcata cctccatgct accttcgggt cttgcggatg aggcatcgtt tacaaacaat
     2461 gaccttaaag cccaaagaat cctatcggca tcttcttcac tgaagcccgt taattccgca
     2521 agctggcagt ttatgctgcc gtagaagaca taaagaccaa agggtacacg gtgttttgtg
     2581 cccattgtat cggggccttt cttctctccc ggaacgctgt tgacgctttt tgtaatctga
     2641 atgctctgaa tgtctatcgg gacaacgcta aaggcgggat gaattgacac cggtcctctg
     2701 actccgatgg aaacatcgct gcccttaaag gcaaatacct gaccaaagct tctgacatca
     2761 atccatgtct cgcaggcaat ttttgcaaat gcttcggggt cttttttctt ttctgccgct
     2821 ttcagctctt caaccgaatc tgccctttct ctcagactct taaagccctc cacagtctaa
     2881 tcatccgact gtacaaataa cttgatagaa ctatcctctt tgctaaggtc aataaacctg
     2941 ttcctgattt tccttttaat acatacatcg gaaatctcac caaaaccgtc atagtcttcc
     3001 cttgggcggt tgccgtttaa ggggtctccg ttgggatttg cgtttttaac cgagacgata
     3061 acagcaaaat cgattttgtt tttcaatacg ctcatatcaa ttctccttca taaaaaaatt
     3121 tattcttcaa aagtttcggt ttcattttgt ttatgagtcc tgctgccttc aataaaatga
     3181 ttcatttgcg ccgagtatcc gagcaggaat gtttcgttaa gaggagtatt gttgaattcg
     3241 gtgtcaagcc gagatattaa tgccatcatt tccgactcaa ggaagaatcc ttttccgcca
     3301 agtcttttta gatacggctg cagttgcttg tgaagtatat cgcagtattt cgcaggagcc
     3361 ctgcagaacg cattgagcaa tctttcagca tttgtctgcc tgttctcttt tgcatcatac
     3421 aaggctttac tttcaatctc ccgaatatat gcaaggattc tgccgaacag atagtttctg
     3481 tcgttgttct caaaatcgag ccccatctta aatacctcct tatttttgct tttatcgttg
     3541 taatactttc tgattaaggc gcaagcaatg ctcagggttt tacggtactc ccagtcttct
     3601 ttgaaacttg caggattgga agccctcctt gcggcgttct tcataatatc aacgggaact
     3661 tttgctccgt ctattataca gggcagcagc ctttcaataa ttgatttttt gtggttttcc
     3721 tttatgtttg tcccgtatgc cgcttctgca atgtctttag gagcgggtgc ccctacaaat
     3781 gcgaattttt catacttttc tttgcctttt tcatcttttc cggttttgat ttttcggtat
     3841 tcgagcttcc atgagcaggt tttatgccat ttttcaatgc gttcaattaa atcaaggatg
     3901 agcagttccc tgtaataaaa aatcgatagc ctaccgcgaa ttgcggacct accggaagtt
     3961 gcggaatcca ttcccataac gacagcctga ttatcctctt tcaagttcgc cttatagccg
     4021 ttaattgctt tatttaattc atttgcgaat ttttcatttg taaatactac cggttcatct
     4081 gaaggataca aggcggcaac aaggtccagg gaatcggcgc aaatgggcgg tatttcttca
     4141 tttttggtgc cccatgcaac aatcacttgt gagtcatgat aagtgccttg ctttgaaatc
     4201 agccagcgca aagcattatg agccttttcg gttgtttccc ggcctatgca gaacgcttgt
     4261 tcaggctttg caaacctgcc tctgtatgta aagtttgaag agtcgttact ggaaataagt
     4321 tttgcggagt cgcctggatt tcttatcttt tttggactta gtcttgatat gggaacgaaa
     4381 tttctggtgg cataacaata atcactatcg ctttcaaggc tgtcctgata gcagataaaa
     4441 ctgttccaaa ggcttttgtc aagccaggga tatatgtcag cgttgtcaga accgtcggaa
     4501 ggtataacag caattcggac aaaggcttta tcctgtgaat ttacgacttt aaatataggc
     4561 ggcgggtcgg ggtctgtcca tttgtcaata agcaggttgt tttcattcgt aaaaagaata
     4621 ccatcatcaa tcaaatcttt tataagagtt cctttttcaa ggtacctcat tattgaaact
     4681 attttaggat gtttgtaagg cgattcgcac cataagcgaa gattttttat atatttatcg
     4741 aatttgccat cctgatttgc atatttcttg taatcacccg caaggtaatt tagcttgtca
     4801 aacagcgggt gaggggccct gtcgtttgtt cccaaggcgg agtcttccgt gcagggtatt
     4861 attgtagttg cgtcgccttt gtcggtgata actctggacc tgccgtataa gaagtttccg
     4921 tcgctgtcta taaggatttc aatatgggct tgttgtgttg tgtgataaat aggaagaaga
     4981 ggagttttat cctgatagaa aacacctact tcagacaggc aattgtcata ggtttcagct
     5041 aatttttgcg cccagcccat ttcattgtga gtttcgcgct gtgtatagta atagtaataa
     5101 aaactgtcca ttgccttaac cgataataaa tcgttgtcaa atctttcagg tttttttcaa
     5161 aggccgccaa tacattgaga gcggcctttt gcccttctct aatatccacc aatttgtcaa
     5221 tgttctcttc tttgaatctg atgatataaa catcaccatt ttcaagctct ttgttgcggt
     5281 tgcacctgcc tgctgcctga gcaatactgt caagccccgc aagagaccgt attacaatgg
     5341 agaaactaat atccacgcct gcctcaatca gctgagtgct aatgcatatg acttcttttt
     5401 catatcttat tttctctaaa acctttctcc tatgggcagg gcaaaggccg gtactcaaat
     5461 agcaaacatt aaaactcgga tagttttcct ttaagcataa aaaggctttt aaagcagctt
     5521 tcttagtgtt taaaatcgca agtatgcgtt tattgtcctt gtgcttttcc gcaataaatt
     5581 ccgcaagccg ctctgatgaa aaaacaccct cgattgtaag gtcccgaatt tgtgttcgtt
     5641 taaatctctt aaatatttcg gtggtatcct caatcattct tttattgctc aacatcagag
     5701 gatacttttt tgtaaggtgc aggcggggct gagttgctgt acataaaacc acagaacagt
     5761 tcatgaccga agctaagaaa ttcatcgcag tattaaacat ataaatgcag tttacgggga
     5821 tggactgaac ttcgtcgata atgattaccg agttagcgag cgaatgaaat cttctggcag
     5881 cggaggactt cccggcaaac aaggtatcga gaaactgcac catagttgtt aagacaatct
     5941 ttgacgacca tcgttctgta agaagcttgt atctgtcatc actgctaaca atatcactgt
     6001 gatgctcaag gatttcttct ttgcagtatt tagaaatgcc cctgacaaca tcagcgttct
     6061 gctcaagtat ggacttatag ggtgcaatat aaaaaatcct gtccttgccg ttcatttttg
     6121 cgcaattaac ggcatacctc agactgctga gtgtttttcc ccctccagtg ggaacatgca
     6181 gctgatatat gccgttatca tttttagcaa agctaaggca tgcgtcagat atcttttgac
     6241 gctctttatt tactgaaccg cttttgtcga gttttctgat gtgattttcg agctcatcgc
     6301 tgagttttgc aaaaaagttg tcgtctgtca tgtttgttac cttttcgttg ttttcaaaaa
     6361 gataactgtc atatctgtcc gcatcgagca cacagctgag caaatatctg acaattaaac
     6421 ctaaagaaaa cactaaagag gctttatcat tagggtacag ctttgccagt ttgttgaaaa
     6481 tgttctctat ttccgtagct gagctatcga aatcatcgga gagtatctct ttatcatgag
     6541 caattttttt tagcacttca tcataaaata tttccctttt gggattaatt ctgacttcaa
     6601 aaacatcttt tccgtccggt gaaataatgt cataaagtcc gttatggtgg tgagaaatta
     6661 ttgcaaggct aatcatttga gcggcaagcg ttttaaatga attctcttta gaagaaaaag
     6721 agtatgccag tttaccgcct gcacttgaat gattaatctc acctcttttt tgtcactctc
     6781 tcccaaaaga tactgctgaa actcttcggt cgcctttccg atatcatgca gtttgccgca
     6841 aagagaggca gtttttgcaa cgccgatttt agcgccaaaa gatttgcaga actcttcaac
     6901 atttttgctg tgctcgcaaa gagtctggat tctggtatct tttgtttttc gtgcgtagta
     6961 tgtattcata ctcaattcct gcataatacc atcccaaact gcatcaaata tttaagtaat
     7021 atgtaactat atgtataaat taccatattt cgagaaaagt caaagggaaa agtcatggat
     7081 atagtaccaa taatgggtca ctcaaaagtc taatcaggaa ttaagagtgt aagcataatc
     7141 tgagaaaata taaaaaaccc cgacagggaa ttacctgtcg gggttttggt gccgatgatc
     7201 ggacttgaac cgatacgatg ttgccatcga gggattttaa gtcccttgcg tctgcctgtt
     7261 ccgccacatc ggcataccta aaatgcttaa taattataat ataagtgccg tcacctgtca
     7321 ataaaagttt gtaaactgca tttattttct taatgtaaaa tttagttgac cgattataca
     7381 tattatggta tcttgtcata atatgcagcc aaatagaagg gtaggggata ttagtgtcag
     7441 agtcttcatt aataaacagg cggcagtaca gcgcgcttta caaaatcctt gccgtaattg
     7501 tcgcggcctt cggaacgggc tttatgtgga gggcgagagg cagccacggc tttggcggaa
     7561 tgttcggaat gttcagcgtg gcggcgttta tgaccctgtt aatcctcgcc atattcggcg
     7621 acagaaaaaa gaatatttat gagatgattc cccttatggt cttcgctaca ggagtaacgg
     7681 caggcggctg gggaacactc aacagacaga tggccggtgt cctcgatacc acctccgttt
     7741 actccacact gggagaatcc acatatgtga atataagccc tttcagcgga atgcttataa
     7801 tgcttatgct tggctttggc tggctgccct ttttcggatt cctgcttgga cgctttttta
     7861 ccgataaacg ctacaaggta aagcacttaa ttatcgcggc attagtcttt tactccgttc
     7921 agtacatatt aaaggcaacg cttgcccacc cgattttgga gctgttccac ccccaggcag
     7981 cggagctttt cgcaaagggt ctcgctgagc agggacttga cacaaaccag tggctgatgt
     8041 acttaaagca ttttaacgga atgtcatggg ccaagaaaat cacaggcggc aggaattatt
     8101 tcacctcaat tgaggttgta tccaaggcta tagccgccgc cgctgtaatg ctgtatcagc
     8161 gctttggagt taaagacaag cggggcagcg ttataaactt ctgtacctgc gcgattatgg
     8221 ctgtctccat aaccttagcc gatgtaataa tgctctttga tgtcccgggc ggaatctttg
     8281 agcacataaa aaagccccag tggcttaaat acacatggtc atactgggag tatttcacag
     8341 gcttccttct cggcttgggc gttatgatac tctgctttat tgcggataag aagcttaaag
     8401 acaattggaa cttagccgac gatttcccga ctatatcccg caagctccgg gcggcatata
     8461 ataccctcac aacccttttc ttcacattcg gtctggcgct tgcaatgccc gccatcagca
     8521 gagtttatga ccaagagtat accttaacgg gaaagaaggt tttctacagc cttgaagact
     8581 acaacgcggc aattcagaca aatcccgatt tgagggctgt ctgctttaaa aatcccatcc
     8641 ctattgccgt aacctatgtg gttgtgcttg cggcgctcct cgcagtatat gcctatatcg
     8701 tgcataagaa catgattaag aaggggcttg atacacccat tgacatgagc cccgtgagat
     8761 tcgcgggcag agcatacata acctacttct taattgtcgc ggctctctac ttctttacaa
     8821 acggcgcggc gctcttaagc cccgttaaag actccgtaac aaagacgatg cttgtatcct
     8881 ttgcggtggt gattctgggc ataataggct taaaactctc agacagacaa gtaaagtcaa
     8941 aagcatagca aaaagaaaag ctcccttaaa agggagctta atttattttt tctttttgcc
     9001 gaaaaagcct ttcttgtcat cctcaggctc acccaggggc ttgtttccca tttcctcatt
     9061 gaactggagg aggatttctt tctgcctctt tgtaaggttg cggggcactt caacattgat
     9121 tctgagcagc tggtcgccca cgcctctgcc gttcaggtgg ggtatgccct tgcccttgag
     9181 tttcagcacg ctgccgggct gagtaccggc gggcagcttg aattttacct tgccgtcaag
     9241 ggtcgggact ttcagctcat cgcccaatgc cgcctgaaca aaggtgacgg tgatttcgca
     9301 ccagacattg tagccgtccc tttcaaagaa gggatgcggt cttacatgta tgtttataat
     9361 caaatctccg gcgggaccgc cgtttaagcc cttgttgccg ttgcccctgc tgcttattgc
     9421 ctgaccgtcg tctatacccg cgggcacatc aacctctatg ctgacatttc ttctgattct
     9481 gcccgtaccc ttgcaggtat tgcacggcgt gggaataaac actccgctgc cgccgcactt
     9541 ggagcaggtt ctcatggttg taatcatgcc gaagggtgtg cgctgctgga ttcttacctg
     9601 acccgagccg ccgcactcag agcatgtctg agaatgagtg ccgggagcag cccttgagcc
     9661 gctgcaggta tcgcaaacct caatgcggtc aacattgacg gtctttttgc atccctttgc
     9721 agcttcctca aaggatatgg tgagggtggt gtgaatatcc gaacccctct gaggagcgtt
     9781 ggggtttgca cgcctctgac ccgtgccgaa tccgccgaaa aagctggaga atatatcgcc
     9841 caggtcaaag tcaaagccgc cgaagccgcc aaaaccgtcg gaggaatcga agccgccgaa
     9901 gccgccgctg ccgcgggaac cccaggtgga gcggcccatc gacccggcca tatggcccag
     9961 gagcaggccg gtgagcaggc cgctgaagcc gctgccgtag tagcc
//
LOCUS       DAONVQ010000072         9869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5287_length_9869_cov_4.144182, whole genome shotgun sequence.
ACCESSION   DAONVQ010000072 DAONVQ010000000
VERSION     DAONVQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5287_length_9869_cov_4.144182"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2040)
                     /locus_tag="PLD98_05720"
     CDS             complement(<1..2040)
                     /locus_tag="PLD98_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05781.1"
                     /translation="MLYLVLGRARSGKTEFIRNLIADFIRVGRENLILLVPEQFSYES
                     EKAMLSRLGADKMQSLDILSFSRLGQKLREKYEPSSPSEPDKGLAAATMCMVLSSLSE
                     SLKFYKDSCANEQIISELLSLYNELRALGVSPENLRSTGESLSSDSLREKTAEVSFIF
                     STFEAILKQTVPDGDAMSQLIRLSREHKPFEKKLIFLDAFAGFTKQELYVIELMVRQA
                     DAVYVSLTSEFLYDEAPGIGPFGHTVRTINQLREIAKRAGVPFGKPAYLSGPSKYNNF
                     PPILKRYNSPELRALEKNIYSVNPHVYSLPADNITIACAANYMEESEYIALTAKKLIR
                     ERGIRARDIVVIERMPNQMRTAVSAAFKKYGIPVFEDNLRPLDTDPLMQFVLSAARIA
                     AEGFGTELIMRFLKTGLTPLSVEDISLLESYVLIWQIDKSEWLTEWTKHPDGYGFEFT
                     DKDKTTLNKINELRLAVVEPLLELKAALEKGSGKECAKALYEFIEEMDAREALKEYAI
                     LLNDSGNMPYALECERVWDILMQSLDDIANLLGDEYYSPREFYAILNTLISSREIGSI
                     PKGLDAVTIGSADRIRTSSPKVVFIAGANEGEFPAPLESSAIFTDRERRALLNAGIEL
                     ACADEYKAAQERFIVYSALTAATDSLYITYSLATFTGAEKLPSEIVTMVESLFPKC"
     gene            2217..3914
                     /locus_tag="PLD98_05725"
     CDS             2217..3914
                     /locus_tag="PLD98_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA05782.1"
                     /translation="MKTVHHKADFCVVGGGMAGTIAAIAAARHGLKVVLMQDRPVLGG
                     NASSEIRMWVCGARGKDNRETGILEEIILENHYRNEDRVYTVWDSVVYEKAYLEKNIT
                     LLLNCTCIDAKREENRILSVKGWQMTSETYHIVEAEYFADCSGDSILAPLTNAKYMLG
                     RESRDDFGESLAPAERDNKTMGLSCLLQIRETDSPKKFIKPEWARTFKTDEELNFRNH
                     RMGTNFWWIELGGDRDSIHDTDDLRHELLSIAFGVWDHLKNQGDHGFDNWVLDWIGFL
                     PGKRESRRYVGEHIITQNDVEAGGKFSDTVAYGGWPMDDHNPLGFNHRESGGNVNHPC
                     PSPWGIPWRALYSVNIENLLFAGRNISATHIAMSSSRVMGTCSLMGQAVGTGVAIAVK
                     SGKNVRNIDIPLLQQTLLYDDCFIPGVKRELSMLTKSAKTNSETVRNGFDRDYDGRNN
                     AWEGKKGERIEYSFDSPVFVEELRLIFDSDLNRNYLNMPCIYPLKEEKYKIPKTLIKS
                     YRVLAEIDGEEREIISVDNNRKRFILHKINERLSKISIEPLKTYGSEIFRVFSVDVK"
     gene            3948..4481
                     /locus_tag="PLD98_05730"
     CDS             3948..4481
                     /locus_tag="PLD98_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HQA05783.1"
                     /translation="MNIWHDISMDRITPDDFIAVIEISKGSKNKYELDKETGLMILDR
                     VLYTSTHYPANYGFIPKTFAEDNDPLDVLVLCSEPIYPASLVRCYPIGVISMQDEGYI
                     DDKIIAIPFGDPMYNCYSTIDQLPSHIFDEMIHFFSVYKTLEGKTTALSDARGKDEAI
                     DIIKQSVKRYKEVFTQG"
     gene            4552..6429
                     /locus_tag="PLD98_05735"
     CDS             4552..6429
                     /locus_tag="PLD98_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05784.1"
                     /translation="MAKKLRALIAVLLCVCLTLGVMSTAAAKAPVDTYGLDRYIEKAL
                     AEKEEIKDTINLGINKPKPVFPKIDDVLSDHTGNALTKLISALWQYLEAFLNYIVASI
                     YGIIPKNNNWVDIDDYVSENFYAGHSEFLDAPIEGSVWRLGYSQKSLIPDDFGKRPYI
                     MGGYDIGKVTYSTYDDIKVRTICIDDGSGRGVSAFAVIDTVGFANTDVRKIRELLKDF
                     ARENNIVSINIGVTHTHSGFDTQGIWAAQPEQIVKAVSANLKGEKLDTSVDKHLLEVI
                     YTRTAASIREAFYNMEEGEMYFAKKNAAGYFSDKSDPSIFIEDIYRFRFAPFSGGKQT
                     IIANFGAHPETVGLKTDDNPGDVLSADFVYYTEQVINKAGYNFMYFQGAIGSLISANR
                     FLSNDGLRLNRYEQTVRYGEEIGYFILGMTLTEEECRTQVADARRESEGMSQSDSYTL
                     WYEGWVPVSERKLPPVFNVAFKEFTYILDNPLIVTVGKLGMANNIMLLDKDNNICTVT
                     EVGFIEFGTDVKVVVMPGETAPELIIGGSTLTAEGSARGKDFVYPALVRMIDDCDLLT
                     FNVMNDSVGYIVPDNDYGIATLRYIDGELTYNADGVLSFGSTAASTFITEFLTLVREF
                     R"
     gene            complement(6469..6900)
                     /locus_tag="PLD98_05740"
     CDS             complement(6469..6900)
                     /locus_tag="PLD98_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007058891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQA05785.1"
                     /translation="MLKYIWPIVIIVTANTVYHITSKSTPADVRPFASLTITYTVAAA
                     LSLLMFYITGSGKGFMGEMQKINWTSIVFGFAVVALEFGSISLYRAGWKISLGSLVAN
                     ICLACVLVIVGVLLFKEHISARQLIGIGLCIAGIILISNNK"
     gene            complement(6951..8312)
                     /locus_tag="PLD98_05745"
     CDS             complement(6951..8312)
                     /locus_tag="PLD98_05745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQA05786.1"
                     /translation="MPKLSVIIASDSHWEHIEDTIESLENQTSDDFEVIFIRPQKESE
                     AEKPIADFCSRAEGRFDVIEECGITPKLFNRGLIAASGEYIMFLTPGDFLPPDSIENF
                     LYAAESRADIITGRFWLNRENSEPGYNKFADAVVLRPEIDRFSAYLPRCHELGCRIFR
                     IRSLQKHNIEFPLVPVMYDYLFFMRAVLSGMKISGTPYGILYRKIRTISDFDIREGLP
                     TKENLQITLDAFKEIEKLAILRITEETGIFEGDEYYIQELLDSQISYLIDCFYRRIWL
                     MDDETLGLLKSAIGEMMEKLTDSRKERLKEEHKDISFPFMYDSIQDAANEPTFSFILD
                     VPKGSAKELIDSFYLQTFPFFELFVSESVADSGRAGDRWLDSPNFYIAPDRNFHAVAR
                     AHAKSKTIILIKDNTPLDSEVLKATHSAEVPNALKSAIFTKNRIQKRMRTSLKNKGAS
                     LKD"
     gene            complement(8351..9859)
                     /gene="lysS"
                     /locus_tag="PLD98_05750"
     CDS             complement(8351..9859)
                     /gene="lysS"
                     /locus_tag="PLD98_05750"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQA05787.1"
                     /translation="MAEEKNLNWEVTANTEENTNELREIRLKKLQELKEKGMDPFQIT
                     TAEHNISAKEIVERFEELENKDVAICGRIMTWRDMGKANFIDIHDRTGRMQIYVRRDD
                     IGDEAFAEFKRWDIGDIVEVKGFVFRTKRGEISVHCKSIRLLTKSLLPLPEKFHGLKD
                     QDTRYRQRYLDLIVNPEVREVFFKRSKIIAKIREFLDSRGFLEVDTPVLHTLEIGAAA
                     RPFITHHNTLDIEMYLRIETELYLKRLIVGGFEKVYELGRIFRNEGMSVKHNPEFTTV
                     ELYQAYADYKDMMNLAEDLCVFLTKEVCGTETITYQGVEINMAKPWTRMTMAESVKKY
                     AGVDYYEWKSDDDARAAAKAKGLEPERPDMTKGEILALFFEEFVEENLIQPTFIYDYP
                     VDISPLAKRKPNEPDMTERFEFFIYAREMGNAFSELNDPIDQKERFVKQVEQRRKEGA
                     NAQVDEDYITALEYGLPPTGGLGIGLDRLVMLLTDSASIRDVLLFPTMKPLN"
BASE COUNT         2456 a         2457 c         2458 g         2498 t
ORIGIN      
        1 gcatttgggg aaaagactct ccaccatggt gacgatttcc gaggggagct tttcggcgcc
       61 tgtgaaggtc gcaagggagt aggttatata aaggctgtcg gtggcggcgg tgagggctga
      121 gtagacgatg aatctctcct gagccgcctt gtattcgtcg gcgcaggcaa gctcgatgcc
      181 cgcgtttaag agggcgcgcc tttccctgtc ggtgaaaatg gcgctgcttt caaggggcgc
      241 ggggaactcg ccctcgttgg cgcccgcaat aaataccacc ttgggggagg atgttcttat
      301 cctgtccgcg ctgcctatgg taacagcgtc aagacccttg gggatactgc ctatctcgcg
      361 gcttgaaatc agggtgttta gtattgcgta aaactccctg ggggagtagt attcgtcgcc
      421 taagagattt gctatgtcgt caaggctctg catgagaata tcccataccc gctcgcactc
      481 aagggcatag ggcatattgc cgctgtcatt gaggagtatt gcgtattctt tcagagcctc
      541 ccttgcgtcc atttcctcga tgaactcata aagagccttt gcgcactcct tgccgctgcc
      601 tttctccaaa gcggctttaa gctccaaaag cggctcaaca accgcgagtc ttagctcgtt
      661 tatcttgttg agcgtagttt tatccttgtc ggtaaactca aagccgtagc catcagggtg
      721 ctttgtccat tcggtcagcc actccgattt gtctatctgc cagataagca cataggactc
      781 aagaagggaa atgtcctcaa cgctgagagg cgtcagtccc gtctttaaaa agcgcattat
      841 aagctctgtg ccgaaaccct cggcggcgat tcgggcggct gagaggacaa actgcatcag
      901 cgggtcggtg tcaaggggtc ggagattgtc ctcaaaaacc ggtatgccgt actttttaaa
      961 ggcggcggag acggcggtgc gcatttgatt gggcatgcgc tcaataacca caatgtccct
     1021 tgccctgatt ccccgctccc ttatgagctt ctttgcggtc agggctatgt actcgctctc
     1081 ctccatgtag ttcgccgcgc aggcgatggt gatattgtca gcgggcaggc tgtagacatg
     1141 ggggttgacg ctgtaaatat tcttctcaag ggctctgagc tcaggggaat tgtagcgttt
     1201 tagaatcggc gggaagttat tgtactttga ggggcccgaa agatacgccg gcttgccgaa
     1261 gggtaccccc gcccttttag ctatttccct gagctgattt attgttctga cggtatgacc
     1321 gaaggggcca attcccggcg cctcgtcgta aaggaactct gaagtcagac ttacatacac
     1381 agcgtccgcc tgacgcacca tcagctctat gacgtaaagc tcctgctttg taaagccggc
     1441 gaaggcgtca aggaaaataa gctttttctc aaagggtttg tgctctcttg agagccttat
     1501 aagctggctc atggcgtcgc cgtcggggac ggtttgcttt aaaatcgcct caaaggttga
     1561 gaagataaat gaaacctcag cggttttctc ccttaaagag tcggaggaaa gagactcccc
     1621 cgtgcttctg agattttcgg gcgaaacgcc taaagctctc agctcgttat acagtgacaa
     1681 aagctcggag attatttgct catttgcgca tgaatccttg tagaacttta agctctcgga
     1741 caaagaggac agcaccatgc acattgtggc ggcggcaaga cccttgtccg gctcggaggg
     1801 ggatgagggc tcgtatttct ctcttagttt ctgccccagc cttgaaaagc tgagtatgtc
     1861 aaggctctgc attttgtcgg ctcccagacg gctcagcatt gccttttcgc tctcatatga
     1921 aaactgctcg ggcacaagga gaataaggtt ttccctgccg acccttataa agtcggcgat
     1981 gagatttctt ataaactccg tttttccgct ccttgctctg ccgaggacca gatacagcat
     2041 atataaacac ttcctttaag taaaacctgc aaaattcatt atacagaaat ttaaggcaaa
     2101 tggaaatagt accctgacaa aagcctaaat tctgtaccat tatcgcccca taaacattta
     2161 cttgaagctg ttcaggtggt atattgatat tagaaagaag ctaagaaggt gatttattga
     2221 aaaccgttca tcataaagcg gatttttgcg tagtaggcgg cggaatggcg ggcacaatag
     2281 ccgcgatagc cgctgcccgt cacgggctta aagttgtgct catgcaggac agacccgttc
     2341 tcggcggcaa cgcctccagc gaaatcagaa tgtgggtctg cggcgcaagg ggcaaggaca
     2401 acagggagac gggaattctg gaggagatta tcctcgaaaa ccattaccga aacgaggaca
     2461 gggtctatac cgtctgggac agcgttgtgt atgaaaaggc gtatctcgaa aagaacataa
     2521 ccctgctttt aaactgcacc tgcattgacg caaagcgcga ggaaaaccgc atattaagcg
     2581 taaagggctg gcagatgacc tcggaaacct atcatattgt agaggcggag tatttcgccg
     2641 actgctcggg cgactccatt ctcgctcccc ttacaaacgc caaatacatg ctgggcaggg
     2701 agagcaggga cgattttgga gagtctttag ccccagcgga gcgtgacaat aaaacgatgg
     2761 gtcttagctg ccttcttcag attagggaaa ccgactcgcc taaaaagttt ataaagccgg
     2821 aatgggcaag gacctttaag accgacgagg agcttaactt cagaaaccac agaatgggca
     2881 ctaacttctg gtggatagag cttggcggcg acagggacag cattcacgac acagacgatt
     2941 taaggcatga gctgcttagc atagccttcg gagtatggga ccatcttaaa aaccagggtg
     3001 accacggctt tgacaactgg gttcttgact ggataggctt tcttcccggc aagagggaga
     3061 gccgccgcta tgtgggcgag catattatca cccaaaacga tgtggaggcg ggcggcaagt
     3121 tcagcgacac agtcgcttac ggcggctggc caatggacga ccataaccct ctgggcttta
     3181 atcacaggga aagcggcggc aatgtaaacc acccctgccc ctccccatgg ggaattcctt
     3241 ggagagcgct gtattcggtc aatatagaaa acctcttgtt cgcgggcagg aatatcagcg
     3301 cgacccacat cgccatgagc tcaagcaggg tcatgggcac ctgctcacta atgggtcagg
     3361 ctgttggaac tggcgtcgcg atagccgtta agagcggaaa gaatgtcagg aatattgaca
     3421 ttccccttct ccagcagacg cttttgtacg atgactgctt tattcccgga gttaaaagag
     3481 agctatctat gcttacaaag tcggctaaga caaacagcga gacagtaaga aacggctttg
     3541 acagggacta tgacggccgg aacaacgcat gggaaggaaa aaagggcgag agaattgagt
     3601 acagctttga ctcccctgtt tttgttgagg aattaaggct tatcttcgac agcgacctta
     3661 acagaaacta ccttaacatg ccctgcatat atcctcttaa agaggaaaag tacaagattc
     3721 caaagactct cattaagtct tacagagtcc ttgccgagat tgacggcgag gaaagggaaa
     3781 taatctcggt cgacaacaac agaaaaaggt ttattctgca taagataaac gaaaggctga
     3841 gcaaaatatc aatagagcct cttaaaactt atggcagcga gatattcaga gttttctcgg
     3901 ttgatgtaaa gtaaaccaaa aggcagacta aaagggaggg tgatattttg aacatttggc
     3961 atgacatatc catggacaga atcacacctg atgattttat cgcggtaatc gagatttcaa
     4021 agggcagcaa aaacaagtat gagctggaca aagaaacggg gcttatgatt cttgacaggg
     4081 ttctttacac ctccacccac taccccgcaa actacggctt tattcccaaa accttcgcgg
     4141 aggacaacga cccccttgat gtgctggttc tctgctctga gccgatttac cccgcctccc
     4201 ttgtgcgctg ctaccctatc ggcgtaatct ccatgcagga tgaaggctat attgacgaca
     4261 agattatagc catacccttc ggagacccga tgtataactg ctactccacc attgaccagc
     4321 tccccagcca catttttgat gagatgatac acttcttctc cgtctacaaa acccttgagg
     4381 gcaaaaccac ggcgctgagc gacgcaaggg gcaaggatga ggcaatcgac ataataaaac
     4441 aatctgttaa aagatacaaa gaagtgttta cacaaggctg aattttatgt tactatgtaa
     4501 aggcataccc ctgtcctttt ttgggacggc ttaatttaag gaggattcaa aatggcaaag
     4561 aaactcaggg ccttgattgc tgttttgctt tgcgtctgcc ttactcttgg cgtaatgagc
     4621 acggcagcgg caaaagcccc tgttgacacc tacggtcttg acagatacat cgaaaaggct
     4681 cttgccgaaa aggaagaaat caaggacacc attaatctcg gcattaacaa accgaagccc
     4741 gttttcccca aaattgacga tgttttaagc gaccatacgg gcaacgctct cacaaagctg
     4801 atttccgccc tttggcagta ccttgaggcc tttttgaact atatcgtcgc ctcgatatac
     4861 ggaatcatcc caaaaaacaa caactgggtt gacatagacg attatgtcag cgagaacttc
     4921 tacgcgggac acagtgaatt ccttgacgct ccgattgaag gctcggtatg gcgtcttggc
     4981 tattcgcaga agagccttat ccccgatgat ttcggcaaaa gaccctacat aatgggcggc
     5041 tacgacatcg gcaaggttac atattcaacc tatgacgata ttaaggtccg cacaatatgc
     5101 attgacgacg gcagcggcag aggcgtttcc gccttcgcgg ttatcgacac cgtcggcttt
     5161 gcaaacaccg atgtgcgcaa aataagagag cttttaaagg attttgcccg cgagaacaac
     5221 atcgtttcaa tcaacatagg cgttacacac acccacagcg gctttgacac ccagggcatc
     5281 tgggcggctc agccggagca gattgtaaag gctgtttccg caaacctcaa gggcgaaaag
     5341 cttgatacca gcgtggacaa gcatctttta gaggtcattt acacaagaac cgccgcttcc
     5401 ataagagaag ctttctacaa catggaagaa ggcgaaatgt actttgccaa gaagaatgcc
     5461 gcgggctact tctccgacaa gtccgacccc agcatcttta ttgaggatat ttaccgcttc
     5521 cgctttgcgc ccttcagcgg cggcaagcag accattatag ccaacttcgg cgctcacccc
     5581 gaaaccgttg gtcttaaaac cgacgacaac ccgggcgatg tactctccgc cgactttgtt
     5641 tactacaccg agcaggttat caacaaggcg ggctacaact tcatgtactt ccagggagcc
     5701 atcggctcgc tgatttccgc caacagattt ctttcaaacg acggtctgag actcaaccgc
     5761 tatgagcaga cggtcagata cggtgaggag ataggctact tcatcctcgg catgaccctt
     5821 actgaggagg agtgcagaac tcaagtcgcc gacgcccgta gagagtctga gggcatgtca
     5881 cagtccgaca gctacaccct ttggtatgag ggctgggtac ccgtaagcga aaggaagctc
     5941 ccgcccgtat tcaatgtggc tttcaaggag tttacataca tccttgacaa cccgcttatc
     6001 gtcacagtcg gaaaactcgg catggcaaac aacatcatgc ttcttgacaa ggacaacaac
     6061 atctgcacag tgacagaggt tggctttatt gagttcggca ccgatgttaa ggtcgtcgta
     6121 atgcccggtg agaccgctcc cgagctgatt atcggcggca gcaccctgac cgctgaaggc
     6181 tccgcaagag gcaaggattt cgtatatcct gcgcttgtaa ggatgattga cgactgcgac
     6241 ctgctgacat tcaatgttat gaacgactcg gtaggctaca tcgttcccga caacgactac
     6301 ggcatcgcga cactcagata catagacggt gagctgacat acaacgccga cggcgttctc
     6361 tccttcggct ccaccgcagc ctccaccttc ataaccgaat tcctgaccct tgtaagggag
     6421 ttcagataaa aacaaaaaaa cgcacccggc ttttttgccg ggtgtttttt atttgttatt
     6481 gctgattaat atgattcccg ctatgcaaag tccgatgccg attagctgcc gcgcgcttat
     6541 atgctcttta aacagcagca ctcccacaat cacaagaacg caggcaaggc agatatttgc
     6601 cacaagggag ccgagactta ttttccatcc ggctctgtaa aggcttatgc tgccgaattc
     6661 cagagcgaca acggcaaagc cgaaaactat tgaggtccag ttgatttttt gcatctcccc
     6721 cataaagccc ttgcccgagc ctgtaatata gaacattaag agggacagcg cagccgcgac
     6781 cgtgtatgtg attgtcagcg acgcgaaggg ccggacatcg gcgggagttg atttggaggt
     6841 aatgtgatag acggtgtttg ctgtgactat tataactatc ggccagatgt atttaagcat
     6901 aatatcctct cgaaaataaa acagggcatt gccctgtttg tttttttggt ttaatcttta
     6961 aggctcgcgc ccttgttttt aaggcttgtc ctcatgcgct tttggatacg gttctttgta
     7021 aagattgcgc tcttaagggc gttgggaacc tcggcgctgt gggttgcctt taagacctct
     7081 gagtcaaggg gcgtattgtc ctttataagt attattgtct tggacttggc gtgagccctt
     7141 gccaccgcgt ggaaattcct gtcgggggct atatagaagt tggggctgtc cagccatctg
     7201 tcccccgctc tgccgctgtc ggctacggac tcagacacga aaagctcaaa gaatgggaag
     7261 gtctgaagat agaaggaatc gataagctcc tttgcagagc ccttggggac atccaatatg
     7321 aagctgaagg tgggctcgtt tgcggcgtcc tgaatactgt catacataaa ggggaaggaa
     7381 atgtccttgt gctcctcttt cagacgctct tttcttgagt ctgtgagctt ttccatcatt
     7441 tcgccaattg cgcttttaag aagccccaga gtttcgtcat ccataagcca gattcttctg
     7501 taaaaacagt caataaggta acttatctga ctatccagaa gctcctgaat ataatactcg
     7561 tcgccctcaa aaatgccggt ttcctcggtt attctgagga ttgccagttt ctcaatctcc
     7621 ttaaaggcgt caagggtaat ctgaagattc tccttagtgg gcagaccctc tcttatgtca
     7681 aaatcggaga ttgtcctaat tttcctgtaa agaattccgt agggtgtgcc gcttatcttc
     7741 atgcccgaaa gcactgcccg cataaagaac aggtagtcgt acattacggg cacaaggggg
     7801 aactcgatat tgtgcttttg caagctgcgg attctgaata tcctgcatcc cagctcgtgg
     7861 catcggggca gatatgccga aaagcggtca atctcgggtc ttaaaaccac cgcgtcggca
     7921 aatttgttgt agcccggctc agagttttcg cggttgagcc agaacctgcc cgttattatg
     7981 tccgccctgc tctccgcagc gtaaaggaag ttctcaatgc tgtcgggagg gagaaagtcc
     8041 cccggggtca aaaacattat atattccccc gacgcggcga taagcccgcg gttaaagagc
     8101 ttgggggtta ttccgcactc ctcgataaca tcaaagcggc cctcagctct tgagcaaaag
     8161 tcagctattg gcttttccgc ttcgctttcc ttttgggggc ggatgaatat tacctcaaaa
     8221 tcatccgagg tttgattttc aagactttct attgtgtctt caatatgctc ccaatgggag
     8281 tctgaggcaa taataactga cagcttaggc attattgctc ctttcagctc tcagccgcgc
     8341 gggcttacag ctaattcaaa ggtttcattg tggggaagag gagaacatct ctgattgaag
     8401 cgctgtcggt cagcagcatt acaagcctgt ccagaccaat gccgagacct cccgtgggcg
     8461 gcagaccgta ctcaagggcg gtgatatagt cctcgtcgac ctgagcgttc gcgccctcct
     8521 tgcgcctttg ctcaacctgc tttacgaacc tctccttctg gtcgatgggg tcgttaagct
     8581 ctgagaaggc gttgcccatc tctcttgcat atataaagaa ctcgaagcgc tcggtcatgt
     8641 cgggctcgtt cggctttctc tttgcaaggg gcgagatgtc aacggggtag tcgtatataa
     8701 aggtgggctg gataagattt tcttccacga actcctcaaa gaagagagcc aaaatttcgc
     8761 cctttgtcat gtcgggtctt tccggctcta agcctttcgc cttcgctgct gctctcgcgt
     8821 cgtcgtcgga cttccactca tagtagtcca cgcccgcgta cttctttacg gactccgcca
     8881 tggtcattct tgtccagggc tttgccatgt tgatttccac gccctgatag gtgatggtct
     8941 cggtgccgca gacctctttt gtgaggaaca cgcaaaggtc ctcggcaagg ttcatcatat
     9001 ccttataatc ggcgtatgcc tgataaagct cgacggtggt aaactcggga ttgtgcttta
     9061 cgctcatgcc ctcgtttctg aaaattctgc caagctcata taccttttca aagccgccga
     9121 caatgagcct tttaagataa agctcggttt ctattctgag gtacatttca atgtcaaggg
     9181 tgttatggtg ggtaatgaag ggccttgctg cggcgcctat ctcaagggtg tgaagcacgg
     9241 gggtgtcaac ctcaaggaag cctctgctgt caaggaactc gcggattttt gctataatct
     9301 tgctgcgctt gaagaatact tccctgacct cggggtttac gataaggtca agatatctct
     9361 ggcgatagcg ggtatcctgg tctttaagac cgtggaactt ctcgggcagg gggagaaggg
     9421 attttgtaag gagccttatg ctcttgcagt gaacggagat ttctcccctc tttgttctga
     9481 atacaaagcc cttgacctca acaatatcgc cgatgtccca gcgcttaaac tcggcaaaag
     9541 cctcgtcgcc gatatcgtca cgccttacat atatctgcat tctgcccgtg cggtcgtgaa
     9601 tgtcgataaa gtttgccttg cccatatcgc gccaggtcat aattctgccg cagattgcga
     9661 catccttgtt ttccagttct tcaaatcttt caacaatttc cttggcggag atattatgct
     9721 ccgctgttgt tatctgaaag gggtccatgc ctttctcttt aagctcctgg agcttcttca
     9781 ggcggatttc ccttaattcg ttcgtgtttt cttcggtgtt cgcagtgact tcccagttca
     9841 aattcttttc ttccgccatt ataccctct
//
LOCUS       DAONVQ010000073         9822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5311_length_9822_cov_4.585850, whole genome shotgun sequence.
ACCESSION   DAONVQ010000073 DAONVQ010000000
VERSION     DAONVQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5311_length_9822_cov_4.585850"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..2361)
                     /locus_tag="PLD98_05755"
     CDS             complement(91..2361)
                     /locus_tag="PLD98_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQA05788.1"
                     /translation="MDQEQIQSIIAVLEDVYPSLYTSIKNLTAQGMMGAAIATIKPYL
                     DEQKAKLAAREKELKAAQQQISAAERELAASAETLAAADRELKAAERQLNIEKTKLEN
                     SFSTLLAQGATLTQSQLQLLQIKLEKRQEILNLEMNVQNAPENYEKLKTEIEKAEAKY
                     TKDIQNAEYDIEEAEKLLATIPQAKWSIYDRNDTPGYQGYGETALNIRNLANVFPIFF
                     IIVAILICLTTMTRMVEEECTYMGVLLALGYTEKSIMAKYLLYAAIASTLGSVIGIFT
                     GLYFFPYAIFKAYGIMYSMPPIEITYPVGWIIVSFLIGVGSTILATYIACRKDLMSQP
                     ATLMRPKAPRPGRRVFLEKIPAIWNRLSFLSKVTVRNLLRNRRRFFMTVIGIAGCTAL
                     LLTGSGLYHSVGSIMHKQYNDGGISDYDFQVVFENPQTPGRVDLFKDFENDPRVESLM
                     LTSMTSVYGTSEKSDKVHDVYLFVPDIPVELGNYIRLINASNKRQHLSLTDEGALVTE
                     KFARDTNIKVGDNAVINTVDGKTVTIPVVGIVENYIFHFVYITPTTYKNLFGEAPEFL
                     YAIGRFSDSVREEAAAAKEGESTQKTMLATDIMKHKTVKSLAFMSDTAKSFSEIISAM
                     LIVVYVIMGSASALALLVLYNLSNININERIRELASIKVLGFYDREVSAYIYRENIVL
                     TALGIFFGLIAGVFMHRIVVSIAEVDAVMFSRDTTFFNYFFAAIVTALFAFIINMIMH
                     KKMKAINMADSLKSVE"
     gene            complement(2475..3647)
                     /locus_tag="PLD98_05760"
     CDS             complement(2475..3647)
                     /locus_tag="PLD98_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA05789.1"
                     /translation="MSKSLIKSILRSVRSSISRFISIVLIVALGVSFFAGFKATYPDM
                     LETAKKYFKENNLMDIRVQSNLGLTKADVTAISKVDGVEYVMPMRFVDALVLVNGEIE
                     TDIDGSQISARAYGISMKMLEEFSKGYDDGSFMNRPTLIEGRYPTRKNECLVDASKLS
                     TPESYKIGNKITLTGDRQNIEGKLNTTEFEIVGIIRSPYYLSFERGNTNIGSGKVGTF
                     IIIPDEAFNTDYYTEVYVTVRGSGNYDPYSDEYFDYIKPVADAIGNIADDRLQVRANF
                     LQNELPNKIADGKITLAEKKAQLEEELKEHKETLNLLKDLVDNGSARVAEAWAAFEAE
                     FGPRDELLGTNQEKYNQFIRQYNEGTIKLAQAQQEFNEKSQEYKIKRVFMTKPSKK"
     gene            complement(3684..4385)
                     /locus_tag="PLD98_05765"
     CDS             complement(3684..4385)
                     /locus_tag="PLD98_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05790.1"
                     /translation="MSFIELESVYKRYKMGEVTISAADGVTFSVEQGELCVIVGPSGA
                     GKTTVLNILGGIDECDDGKIRVGGELINDYDKKRLTEYRRYDVGFVFQFYNLVPNLTA
                     LENVELATQISNKALSAEKVLERVGLAERMQNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYNTGKQILKLLQETSRETGMTIIIITHNQAITPIADRVITMRSGRVHR
                     IAINDNPRPVELLEW"
     gene            complement(4545..5456)
                     /gene="whiA"
                     /locus_tag="PLD98_05770"
     CDS             complement(4545..5456)
                     /gene="whiA"
                     /locus_tag="PLD98_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HQA05791.1"
                     /translation="MSFSSDVKLELIEQKNEPCCQKAEAYGLLLFSRSFSANSIEFLS
                     EQESLVQKYNDYVVMATGKPAEIKKTPAGNHKLIVRNKEQRLKLLSYFGHDANEVSLR
                     INLANLSDECCSRAFIRGAFLSCGTITDPEKEYHLEFTIPYKNLTKDFVRLCEELGFS
                     PKTTIRQNNHIVYFKDSTNIEDLLTAMGATNASLSLMGIKMYKSVINNTNRRVNFDKA
                     NIKRSTDSAMIYIDAIRTIEKAKGLNSLPSDLKELAMLRLENPDMSQKELAASLSVPI
                     SLSAVNRRLKKLVEIADSIRTSSVKNR"
     gene            complement(5461..6414)
                     /gene="murB"
                     /locus_tag="PLD98_05775"
     CDS             complement(5461..6414)
                     /gene="murB"
                     /locus_tag="PLD98_05775"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQA05792.1"
                     /translation="MQQINPAVLNEIKKSGSEYIENAPMRKLTSFRTGGNADILVYPK
                     TEEEFISLAKICRSCGTPVTVIGNGSNLLVSDNGIRGVVVSTSKLTEIKDDDELTFTA
                     GAGLPLKHFCRHALERSLTGLEFAYGIPGSVGGAAYMNAGAYDGEMKDVILHCRHVDP
                     SGKVSVLTNPELDFSYRHSIYSDSDLWILSVTVRLKKGNREEIESKMQDLMSRRKEKQ
                     PLEYPSAGSTFKRPKGSYASLLIDQCGLKGLSVGGACVSEKHAGFIINKDNATSDDIF
                     SLILRVRQEVFDKTGFLLEPEVKIIGDWSHRNDFDKIKGTG"
     gene            complement(6425..7372)
                     /locus_tag="PLD98_05780"
     CDS             complement(6425..7372)
                     /locus_tag="PLD98_05780"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HQA05793.1"
                     /translation="MYRVGVDLGGTNIVAGVIDENNKILSRASVKTNAPRKPELIFDD
                     IASVVKKAVADAGITLEDVKSIGVGTPGSVNKETGYIEFANNLDFNQVPAAEMLKERL
                     GRTIYLDNDANCAALGEAVAGAGNGVKNFVAITLGTGVGSGIIIDGKLVVGLNYAAGE
                     MGHMVIKFDGEQCSCGRRGCWEKYASAPALIEQTKDAMRNNHDSKMWALVNNNIDAVS
                     GRTAFDAMRQGDQAAKAVVDRYISYVACGIINIINALQPDMICIGGGVGHEGEYLLEP
                     LRRIVERERYSLYAKKQTRLCSAVLGNDAGVIGAALLDE"
     gene            complement(7395..8360)
                     /gene="hprK"
                     /locus_tag="PLD98_05785"
     CDS             complement(7395..8360)
                     /gene="hprK"
                     /locus_tag="PLD98_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HQA05794.1"
                     /translation="MESKYSVSLAKVIRENGFETIYLPADPASIFISSQEVNRPGLIL
                     SGFEEYFEPARVQFLGLMEMKFLSTLSREKRLESFERFFSKRPPVVLITRNLEPIDGL
                     LETAQKYEVPVLRTSDSTSAAMSALISYLSVELAERITRHGVLVEVAGEGVLILGDSG
                     VGKSETAIELIKRGHRLIADDAVEIRRVSKKTLVGSAPDNIRHFIEVRGIGIVNARRI
                     FGTGAVKLTEKIDLVIQLEPWDSNKVYDRIGLDEETTTILGIKVPAIVIPVNPGRNLA
                     VIIEVAAINNRQKKMGYNAAKELMKNLGLHDIETEPVEHDFWQDE"
     gene            complement(8399..9241)
                     /locus_tag="PLD98_05790"
     CDS             complement(8399..9241)
                     /locus_tag="PLD98_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HQA05795.1"
                     /translation="MSNEKLSIGVKQAGAELTSVKSKESGYEFLWQGNPDIWYGQSPI
                     LFPIVGRLADDKYLLNGVEYTIPKHGFARRSKFEFVAKTDDSLVFRLSDNEETKKLYP
                     FEFDLIVTFRLIDNALEVNHKVVNKSADTMYFSLGAHPGFNCEIGDTLRFEQDETLST
                     QRINSESLRIPETYPVLENSKDIVITKDIFNEDALILSGYKSKKITLTNKSSGRTVEF
                     TFGDAPYLGIWAKPGAPYVCIEPWFGVNDSTEKYGEFKNKEGINALQPGEAFEYAWKA
                     VFNG"
     gene            complement(9467..9811)
                     /gene="yajC"
                     /locus_tag="PLD98_05795"
     CDS             complement(9467..9811)
                     /gene="yajC"
                     /locus_tag="PLD98_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQA05796.1"
                     /translation="MLMLIPFMALMYFTIIRPEKKRRKQEQEMRDSVQIGDEITTLGG
                     IMGRVVTIKEDSLVIETGSDRNKIKIARWGIQTNNTAEERLQAEKEAAAAAKAAEKQA
                     AAEALKEKKKKK"
BASE COUNT         2234 a         2316 c         2348 g         2924 t
ORIGIN      
        1 acatacatta caatttgccc cgctgtggta tttgtaacgg gcaaataaaa agaccgcgct
       61 gatttacagc gcggtctgta tttgtgtttt ttactcaaca gactttaagg agtctgccat
      121 gttgattgcc ttcatcttct tatgcattat catgttgatt atgaaggcga acaatgctgt
      181 tacgattgcg gcaaagaagt agttgaagaa ggtggtatct ctgctgaaca taacagcgtc
      241 aacctctgcg atgcttacca caattctgtg cataaatacg cccgcaataa gtccgaagaa
      301 gattccgagg gctgtgagga caatgttctc cctgtagatg tacgcggaga cctctctgtc
      361 gtagaagccg agaaccttga ttgaggcaag ctctctgatt ctctcgttga tgttgatgtt
      421 cgagaggttg tagagaacca agagcgcaag agcggaggct gagcccatga tgacatagac
      481 gactatgagc attgcggaga taatctcgct gaagctcttt gccgtatccg acatgaatgc
      541 gagggatttt acggtcttgt gcttcataat gtcggtggcg agcatggtct tttgagtgct
      601 ctcgccctct tttgcggctg ccgcctcttc gcgtacagaa tcggagaatc tgccgattgc
      661 gtacaggaat tcgggcgcct cgccgaacaa gttcttatat gttgtaggag taatgtatac
      721 aaagtggaag atgtagttct ccacaatgcc cacgacgggg attgttactg ttttgccgtc
      781 aacggtgttg attaccgcat tgtcgcccac cttgatgtta gtatcgcggg cgaacttttc
      841 ggtgaccagc gctccctcgt cggtaaggct gagatgctga cgcttgttcg aagcgttaat
      901 aagccttata tagttgccaa gctcaacggg aatgtcggga acgaaaaggt aaacatcgtg
      961 taccttgtcc gacttctccg aagtgccgta aaccgatgtc atggatgtga gcatcaatga
     1021 ctcgactctc gggtcgtttt caaagtcttt gaacaggtcg actctgccgg gagtctgggg
     1081 attttcaaat actacctgga agtcgtagtc ggaaattcct ccgtcgttat actgcttgtg
     1141 cataatggag ccgaccgaat ggtaaagacc tgagcctgta agaagcagcg cggtgcagcc
     1201 cgcgatgcct attacggtca tgaagaacct gcggcggttt cttaaaaggt ttctgacggt
     1261 gacctttgaa aggaaactga gcctgttcca gattgcgggt atcttctcca ggaataccct
     1321 gcgtccgggt ctcggcgcct tcgggcgcat aagggttgca ggctgtgaca taaggtcctt
     1381 tctgcatgcg atgtaggtag cgagtatggt gcttcctacg cctataagga aggatacgat
     1441 aatccatcca acgggatagg ttatctcaat cggcggcata ctgtacataa ttccgtatgc
     1501 cttgaagatg gcgtatggga agaagtaaag tcccgtaaat atgccgataa ccgagcccaa
     1561 tgtgcttgcg attgccgcat agagaagata ctttgccatt atgctctttt cggtatagcc
     1621 cagagcgagc agaacgccca tgtatgtgca ctcttcctca accattcttg tcatggttgt
     1681 aaggcagata agaattgcga cgattatgaa gaatatgggg aatacattcg cgagatttct
     1741 gatattcaga gcggtttcgc cgtagccctg atagccgggt gtatcgtttc ggtcatagat
     1801 gctccacttt gcctgcggaa tggtggcaag cagcttttcc gcctcttcta tatcatattc
     1861 ggcgttttgt atgtcttttg tgtatttagc ttccgctttt tcaatctcag tcttcagctt
     1921 ttcataattc tcgggagcgt tctgaacatt catctcgaga tttaagattt cctgcctttt
     1981 ctccagctta atctgcaaaa gctgaagctg cgactgggtt aaggtcgcgc cctgcgcaag
     2041 caaggtgctg aagctgtttt ccagctttgt tttttcgata ttgagctgtc tttccgcagc
     2101 cttgagctca cggtctgccg cggctaaggt ttccgcgctt gccgccagct ctctttcggc
     2161 cgcgctaatc tgctgctgag cggctttaag ctccttttct cttgcggcga gcttagcctt
     2221 ctgctcatcc aagtagggct ttatggtggc tattgccgcg cccatcatgc cctgagcggt
     2281 caagtttttg attgaggtgt aaagggaggg gtaaacatcc tcaagaaccg cgataatgct
     2341 ttgaatctgc tcctggtcca aagcggagct ttgggtttcc tcaagggttt ttaagaccgt
     2401 ttctgtcgta ttgattatgt agtttaaaga ctcaatggcg gtggtcgcgg tattgattat
     2461 tgttttgttg gcgtctactt tttgcttggt ttcgtcataa agaccctttt aatcttgtat
     2521 tcctggcttt tttcgttaaa ctcctgctgt gcctgagcaa gttttattgt gccttcgtta
     2581 tattgacgga taaactgatt gtacttttcc tgattggtgc caaggagctc gtccctggga
     2641 ccgaactccg cctcaaaggc tgcccatgcc tcggcaaccc ttgcgctgcc gttgtccaca
     2701 aggtctttaa gaagatttaa tgtttcctta tgctctttaa gctcttcttc aagctgagct
     2761 tttttctcgg ctaaagtgat ttttccgtcc gcgattttgt tgggcagttc gttctgcaaa
     2821 aagtttgccc ttacctgcaa gcggtcgtcg gcgatattgc cgatagcgtc cgcgacgggc
     2881 tttatgtagt caaagtattc gtcggaatag gggtcgtaat tgccggagcc cctgacggtt
     2941 acatagacct cggtataata atcggtgtta aaggcttcgt cggggatgat tatgaaggtg
     3001 ccgactttgc cggagccgat gtttgtgttg cctctttcaa atgacaggta gtagggggaa
     3061 cggataatgc cgactatctc gaattctgtg gtgttcagct tgccttcgat attctgcctg
     3121 tcgcccgtaa gggttatttt gttgcctatc ttatagcttt cgggggttga cagcttgctt
     3181 gcgtcaacaa ggcactcatt cttacgggtg gggtatctgc cttctatgag ggtcgggcgg
     3241 ttcataaaac tgccgtcgtc atagcccttt gagaattctt caagcatttt catgctgatg
     3301 ccgtatgctc ttgcgctgat ttgagaaccg tcgatgtcgg tttctatctc gccgttgacg
     3361 agcacaagcg cgtcaacaaa gcgcatgggc ataacatatt caacgccatc aaccttagag
     3421 atagcggtta catctgcctt agtgagtccc aaattggact ggactcttat gtccatcagg
     3481 ttattctctt taaagtattt tttagcggtt tcaagcatgt ccggatatgt agccttgaat
     3541 cccgcgaaaa agctaacacc aagcgcaaca atgagcacaa tggaaataaa gcggcttatg
     3601 ctgctgcgga ctgaacggag tatgcttttt atcagtgatt tgctcatccg cttatcctcc
     3661 ttaagaataa gatgaatgtc tgactaccat tcaaggagct caacaggtct gggattgtcg
     3721 tttatggcaa tcctgtgcac tctgccgctt cgcatggtga taactctgtc ggcaataggc
     3781 gtgatagcct ggttatgtgt gattatgatt atggtcatgc ctgtttcacg gctggtttcc
     3841 tgaaggagtt taagaatctg cttgcctgtg ttatagtcaa gagcgccggt gggctcatcg
     3901 cacaaaagca gtttggggtt ctttgctaga gctctcgcta tggaaactct ctgctgctcg
     3961 ccgcccgaaa gctgcgaggg gaagttttgc attctttcgg caagtccgac gcgctcaagc
     4021 accttttcgg cgctgagagc cttgttgctt atctgagtcg cgagctcaac attttcaaga
     4081 gcggtaaggt taggcacaag attataaaat tggaatacaa agccgacatc atatcttctg
     4141 tactctgtca gcctcttttt gtcgtaatcg tttataagct cgccgccgac gcggattttg
     4201 ccgtcgtcgc actcgtcgat gccgccgagt atgttaagca cggtggtttt gccggcgccg
     4261 ctggggccga caataacgca cagttcgccc tgctcaacag aaaaggtaac gccgtctgcg
     4321 gcgctgattg taacttcacc cattttatag cgcttgtaaa cagattccaa ttctataaag
     4381 ctcatacact tttcccttcc gtctttttag tacccctaag ttttagactc aagatttatg
     4441 ttaaataatt taattttttg cagaatttgc gattttcgtt aaatttattg taaatttata
     4501 atattacaaa acagacttca agtcaaataa agatattgat aaatttacct attttttaca
     4561 ctgcttgtcc gaatgctgtc agcaatttcg acaagttttt taagtcttct gttgacagcg
     4621 gagagggaaa tcgggaccga cagagacgcc gccagctcct tttggctcat gtcgggattt
     4681 tcaaggcgga gcatggcaag ctcctttaaa tccgacggca gtgaattcaa tccctttgcc
     4741 ttttctatgg ttctgatggc gtcgatgtaa atcatcgcgg agtcggtgga gcgttttatg
     4801 ttcgccttgt cgaagtttac tctgcggttt gtgttgttga taacgctctt atacatctta
     4861 atgcccatca gtgaaaggga ggcattggtg gctcccatgg cggtgagcaa atcctctatg
     4921 ttggtgctgt ctttaaagta gactatgtgg ttgttctggc ggatagtggt cttcggggag
     4981 aaccccagct cctcgcaaag gcggacaaag tctttagtga ggtttttgta tggaattgta
     5041 aattccagat gatattcctt ttcggggtcg gtaatggtgc cgcaggacaa aaaggctccg
     5101 cggataaagg ctctgctgca gcattcgtcg gagaggttgg caagattgat tctgaggctt
     5161 acctcattgg cgtcatgccc gaaataggaa aggagcttta gtctttgctc cttatttctg
     5221 acaatgagct tatgattgcc cgcgggagtc ttttttattt ccgccggctt gcccgttgcc
     5281 attaccacat agtcattata tttttgaaca agactctcct gctctgacaa aaactcgatt
     5341 gagttcgcag agaaactgcg gctaaataat aagagcccgt atgcttcggc cttttgacaa
     5401 cagggctcgt ttttttgctc gattaattcc agttttacat cgcttgaaaa tgacatatcc
     5461 tcagcctgta ccctttattt tgtcaaaatc gttcctatgg ctccagtctc cgataatttt
     5521 aacctcaggc tcgagaagaa accctgtttt gtcaaagacc tcttgtcgta ctcgtaaaat
     5581 aagactaaaa atatcgtcgc ttgttgcatt gtctttattg attataaaac ctgcgtgctt
     5641 ttcgctgacg caagcgccgc ccaccgacag ccctttgagc ccgcactggt ctataagaag
     5701 acttgcataa gaacctttgg gcctcttaaa ggtgctgccc gcgctggggt attcaagggg
     5761 ctgcttttcc ttgcggcggg acataaggtc ctgcattttg ctttcgattt cttctctatt
     5821 gcctttttta agtcttaccg taactgaaag aatccacaaa tcgctgtcgg aataaatgct
     5881 gtgcctgtag gaaaaatcaa gctcgggatt tgttaaaacg cttactttgc cgctggggtc
     5941 cacatggcgg cagtgaagga ttacatcctt catttcgccg tcgtatgctc ccgcgttcat
     6001 ataagcggcg ccgcccacag agccgggaat tccgtaagcg aattcaagcc cagtaagcga
     6061 acgctccaga gcgtggcggc agaagtgttt aagcggcaat cctgctccgg cggtaaaggt
     6121 cagctcgtcg tcgtctttaa tctcagttag ctttgaggtt gaaacaacaa cgccccgtat
     6181 tccgttatcc gaaaccagta gattgctgcc gtttccgata acagtaacag gggtgccgca
     6241 gcttctgcat attttcgcga gggaaatgaa ctcttcctcg gttttcgggt aaaccaggat
     6301 atcagcattg ccccctgtgc gaaagctggt gagctttcgc ataggggcgt tttctatata
     6361 ttcgctgcct gattttttaa tttcgttaag tacggcgggg tttatttgct gcattgaatc
     6421 atctctattc gtcaagtagt gctgcgccga ttacgcctgc atcgttgccg agaacagcac
     6481 tgcaaagacg ggtctgcttt ttggcgtaga ggctgtatct ctccctctcg actatacggc
     6541 gcagaggctc aagcagatac tcgccctcgt gaccgacgcc gccgccgatg cagatcatat
     6601 cgggctgcaa agcattgatt atattaatta taccgcatgc gacataagaa atatacctat
     6661 ctacgacagc ttttgcggcc tggtcgccct gacgcatcgc gtcaaaggct gttctgcccg
     6721 aaacggcgtc aatattgttg tttacaagag cccacatctt gctgtcgtgg ttgttgcgca
     6781 tagcgtcttt tgtctgctca ataagggcgg gggcggaggc atacttctcc cagcatccgc
     6841 gtctgccgca gctgcactgt tcgccgtcaa acttaattac catgtgtccc atttcgcccg
     6901 cggcgtagtt aagacctaca accagcttgc cgtcgatgat gatgccgctt ccgacacccg
     6961 ttccgagggt aatggcgaca aagttcttaa caccgttgcc ggcgcccgct actgcctcgc
     7021 caagggctgc acagttggcg tcgttgtcaa gatatatggt tctgccgagt ctttctttca
     7081 gcatttcggc agccggaacc tgattaaagt caagattgtt cgcaaactct atatatcctg
     7141 tttccttatt aaccgaaccg ggggtgccaa cgccgattga cttgacatct tcaagggtaa
     7201 ttcccgcgtc ggccacagct tttttgacaa cgcttgcgat atcgtcaaaa atcagctcgg
     7261 gttttctggg agcgtttgtc tttacgcttg ccctgctgag aatcttatta ttttcgtcaa
     7321 taactcctgc gacaatgttt gtgccgccta aatcaacgcc aactctatac atttttatac
     7381 ctccgattta tttttcattc atcctgccaa aaatcatgct caacgggttc ggtctcgata
     7441 tcatgcagcc ctaagttctt cataagctcc ttggcagcgt tatagcccat cttcttttga
     7501 cggttattta tagcggcaac ctctatgatt accgccaggt tgcgtcccgg atttacggga
     7561 atgactattg cgggaacctt aatgccgagt attgtggtgg tttcctcgtc aagacctatt
     7621 ctgtcgtaga ccttgtttga atcccacggc tcaagctgaa taacaaggtc gattttctcc
     7681 gtaagcttta cggctcccgt accgaaaatc cttcttgcgt tcactatgcc tatgccgcgc
     7741 acttcgatga agtggcggat attgtcgggc gctgagccta caagggtttt ctttgatacc
     7801 cttctgattt ccacagcgtc gtcggcaata agtctgtgtc cgcgcttaat aagctcgatt
     7861 gcggtttcgc ttttacctac accgctgtcg cccagaatca gaacgccttc gcccgctacc
     7921 tcaacaagaa cgccgtgcct tgtgattctc tcggcaagct ctacgctgag atatgaaatc
     7981 agcgcggaca ttgcggctga agttgagtcc gaggtcctga gaaccggcac ttcgtatttt
     8041 tgcgccgttt ctaaaagtcc gtcaatcggc tcaagatttc gggttatgag caccacgggc
     8101 ggacgctttg aaaagaaacg ctcaaagctc tcaaggcgtt tttcgcgtga taaagtgctt
     8161 aaaaacttca tctccatgag ccccaaaaac tgaactctgg cgggctcgaa atattcctca
     8221 aagcctgaaa gaataagtcc gggtcggtta acttcctggg agcttataaa tatgcttgcg
     8281 gggtcagcgg gaagataaat tgtctcaaag ccgttttccc ttatgacctt tgcaagcgaa
     8341 acggaatatt ttgattccaa atcctcactc ccctttttgt agtctgactg ctttcccttt
     8401 agccgttgaa tacagctttc caggcgtatt caaaggcttc gcccggctga agggcgttta
     8461 tgccctcttt attcttaaat tcgccgtatt tctcagtaga atcatttact ccgaaccaag
     8521 gctctatgca tacataggga gcgcccggct ttgcccatat gccgagatac ggagcgtcgc
     8581 caaaggtaaa ctccacggtt ctgccgcttg atttattggt aagggtaatt ttctttgact
     8641 tatagcccga aagaataagc gcgtcttcgt tgaaaatatc cttggtgata actatatcct
     8701 ttgaattctc gagaacggga taggtttcgg gaattcttaa agactcagaa tttatacgct
     8761 gggtggaaag ggtttcgtcc tgctcaaatc tcagcgtatc gccgatttcg cagttaaagc
     8821 cagggtgggc gccgagggag aaatacattg tgtcggcgct cttgtttact accttgtgat
     8881 ttacctcaag ggcgttgtcg ataagcctga aggtaacgat aaggtcaaac tcaaagggat
     8941 agagcttttt tgtctcttca ttgtcggaaa ggcggaaaac aaggctgtcg tcggttttgg
     9001 cgacgaactc gaacttcgac cttcttgcaa agccgtgctt aggtatggtg tactctacgc
     9061 cgtttaacag atatttgtcg tcagcgagcc tgcccacgat ggggaaaaga atgggcgact
     9121 gaccgtacca aatatcggga ttgccctgcc acaaaaactc atatccgctc tcttttgatt
     9181 tgactgaggt gagctctgcg ccggcttgtt ttacgcctat tgagagtttt tcattactca
     9241 aatgacaaac tatcaaatcc actcaacacc tttatgaaaa ttctaaaata ttgtacactt
     9301 taaagtgttt atttgcaagt atattcatgc attaaaatgg ataaaaactc tgatattgct
     9361 aagattttgt tgtatttttt tatttactct gcctaaaagg atttatgtca aaactcgacg
     9421 caaacaaaaa agccgcagta agtgctgcgg ctttgtactt ctttgcttat ttcttctttt
     9481 tcttttcttt aagagcttct gctgcggcct gcttttcggc tgcttttgcc gcggcggctg
     9541 cttctttctc agcctgcaaa cgctcctcgg cagtgttgtt ggtctgaata ccccagcggg
     9601 caatctttat cttgttcctg tcggagcctg tttcgataac aagcgaatct tccttaatgg
     9661 taacaactct gcccataatg ccgcccagtg tggtgatttc atcgcctatc tggacggaat
     9721 cgcgcatttc ctgctcttgc ttgcgccttt tcttctcagg ccttataata gtgaaataca
     9781 taagagccat aaaaggaata agcatcagca aagaagcaaa gg
//
LOCUS       DAONVQ010000074         9809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5321_length_9809_cov_4.368567, whole genome shotgun sequence.
ACCESSION   DAONVQ010000074 DAONVQ010000000
VERSION     DAONVQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5321_length_9809_cov_4.368567"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLD98_05800"
     CDS             <1..359
                     /locus_tag="PLD98_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQA05797.1"
                     /translation="YFLTILLGGPICKLFGADSETTDLAVKAIPLNSWSFIFAGLNTI
                     ISAYLYSTKRTKESAAVNVLRGFILSPICVFLLSMAFKDAIIWHTIGIAEALTFIAAL
                     LIVKNSEKHGVDFNKK"
     gene            complement(492..1463)
                     /locus_tag="PLD98_05805"
     CDS             complement(492..1463)
                     /locus_tag="PLD98_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQA05798.1"
                     /translation="MNLMALLTAAITALSTLLVSIFGYGADLTDTAKYFQSRVKNLQV
                     YENTIETALPQIIVYDLVSEHFDAPLPDGKTEKKAIIIGFDGGRADVLDISSLEVGGS
                     KLLLEQGGKAYLAYTGGVNFPKINRQFTSTAPGWCSILTGKWANEHGIINNGMTKAVE
                     PLTLLTSLVESGKADSTMFQVSWRGHFVNDDSTYKLEKQYCEEKGLNVVFNCNPDDEG
                     THASLMAAVAEADCPDFIMGIFEHLDKAGHSTGFSVNNPKYRKGYAESERAAYELICA
                     IQARETYAQEDWLIVITSDHGGISTMHGGPSIQERMIFIVTNKEIAL"
     gene            complement(1573..1659)
                     /locus_tag="PLD98_05810"
     tRNA            complement(1573..1659)
                     /locus_tag="PLD98_05810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1623..1625),aa:Leu,seq:cag)
     gene            complement(1838..2737)
                     /locus_tag="PLD98_05815"
     CDS             complement(1838..2737)
                     /locus_tag="PLD98_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05799.1"
                     /translation="MKKTLKVVSAMLAVLLILLSFAGCNMFTIGEESTTTTEYSTSLT
                     EKPADTAKAVAYFNSLLEKAVSLPKEQRPDISWEESIKVSDKSIKITSAKLSGAEDET
                     VTLEKDAEELINDLNNIAPIVKNYIIQNLPKQKSATTVPDKNGNATAYEDILYGAGGL
                     SAFDTSYAVVIEDNSDENLYKITIRFNDEYNPTANSSRIGKAFDLRTKDDIFNALNKD
                     AMKGYADVKDYDAVYKNCSIYAVVNRLTDEIISIEYRKNVDVTAKAELLGKLAPAGEV
                     NISFTVENVRKYEFEWPILDEEA"
     gene            complement(2862..4109)
                     /locus_tag="PLD98_05820"
     CDS             complement(2862..4109)
                     /locus_tag="PLD98_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQA05800.1"
                     /translation="MKASESVPAFNSKLREYTNYIIRGIKKICTEIGPRAAGSEQELK
                     AQEFMKSDLEKVADSVTIEPFKVRPKAFMGWFKIVSILLIFGLIVFLAGMPILTFMLL
                     VAAAAILLGEFLFYREFTDALYKPATSHNLVAVRKAKGETKRRIIVSGHMDSSYEWRY
                     THKGGKFLLFFMLGGAAVGFAFFFITTMVALAKGYITEAPAKGYPFVMFIISVCFTPF
                     FIGLYFMLNEKVMAPGANDNLTGSLCSAAVIRFLADNDIRFENTEVIALLTGSEESGL
                     RGAKAFAKQHEDMLKDKNVETIFVGVETLRDYEHINIYSKDMSGLVRNSEKVCALMKE
                     AGKLCGMDLKYASVYAGASDAAEISRQGYHAATLAAMDPGPPRYYHTRLDSHDNLDPK
                     TIEKGLEIVLNTVFLFDEQGLKA"
     gene            complement(4269..5039)
                     /locus_tag="PLD98_05825"
     CDS             complement(4269..5039)
                     /locus_tag="PLD98_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HQA05801.1"
                     /translation="MTEKEKILKYFERIGLEYDENAELNGEYLRKLQLAHVTAVPYEN
                     IDILRGTPLKLDDDSLFDKVVLRRRGGYCFELNGILAWLLRALGFGVTEYMGRFLRGE
                     VGVPMRRHRVLKVEAADGVYMCDVGVGAKAPRCPLLLKEGLVQKQFGEEYRFEKRDFF
                     GWVLTEKTNKGWGDVFSFTEEVQHPIDFVQPSFYCEKHEDSIFNKDYMIAIKTLTGRK
                     TLDKNTFKIFEGSELISAKELSDSEIPVYLKEHFGLDV"
     gene            complement(5282..6484)
                     /locus_tag="PLD98_05830"
     CDS             complement(5282..6484)
                     /locus_tag="PLD98_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HQA05802.1"
                     /translation="MKNAELLSPAGDLECIDAALHFGADAVYAGGRIMQLRAAKAAMS
                     DAALKEAVRRVHEKGKRIYITLNCFAENSDIEKAGEYARELYEIGVDAVIVSDIGVLA
                     EIKRKVPEIEVHVSTQANCMNYMAARVYYDMGAKRVILARELSIESIAELRAKTPKDL
                     QLEAFVHGAMCMSYSGRCLISSFLTGRSGNKGECAQPCRWQYALVEKKRPDQSFDIEE
                     EGEFSAILSSHDLCAIEFLDELRKAGVDSFKIEGRMKTPYYVATVTDSYRKAIDKTYN
                     IKDLRLQLDTISHRPYSGGFYFGELIHNHYNDGEYRRTCDFIAIALEDTNSEGFTLLE
                     QRNFFKKGDVLELLSPDSLNKKVTVKEIRDMEGSERETAHLVQEKVLVNLGESIRKGD
                     MLRRRISE"
     gene            6694..>9809
                     /locus_tag="PLD98_05835"
     CDS             6694..>9809
                     /locus_tag="PLD98_05835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQA05803.1"
                     /translation="MKFKPKGKTKLMSVILAAAMLLSAVPSGLILPALASDTPDVSVA
                     VFTDIGYMTPEEQGVGSEDFEAFRRNSDTLYEYMEGILDSALASVKDKALKGELRYLI
                     VNGSLTATGSEKSHLALAAKLKELEDSTEGALQVIAMAGEGDINASSFSFENGVKESA
                     PGITPSRFKEIYADLGYDLADGLYDAAVSKAGGLTYTVSLNGDIRLIMADVSKYSPDI
                     TSTGTAAAEKGGVIAPELLDYIKAQALDAKVNGQVPVLVSDFAVVPVNHVIESLNSSD
                     FVRNYHKIAEELADAGIHFHIGGKPGYNDIASIVSDEGETIYGILSSALTSFPATYRI
                     IGLTKGEGQTLSACAELVDCDDVQPVVYKGNNVQKPFKKTTALNASFGGSSQAYVEKF
                     IRNLADDFLDKVKAAGGFIKYYEDIKGINIRFMVDSLFFGGISIDGTPVLSGQNVESF
                     LNAIDNQLMKRYLNDRSYTYELVKKVSSRIAGLPASNLPCDKFYKDYRIGSPYRAGTF
                     GDALVSVMLYMTLGNEDKSDDAFINDVINNLYNTSLSEDVYRIALEVLFDDIIKDALK
                     HTKIDISSYFEGEAKKIANYIQLGFEFLMFLIRGDNSYFNLLEIILKNSNTKYGESFE
                     DLKNYIINDSIAKEDFEIVGISAAELIDAAVTDSNPAEKSDHGVCFSYNGKVKVVPTK
                     EELRLPSMKAVTFGENTQTSVNISWYTKLSVTGTDIEIYPANGNNPPMFTGTPTTGAG
                     IRKFTQVVKRSYPAGSFGIFDFGSSEVETRRHVIQLTGLEKGKKYFYRVGDASRGWWS
                     EIGSFTTGAGEGNFTFLYMSESGGKTKAQYEIFANTVKEALKAYPKTSFIMHAGSLTA
                     NGANTRHWQWALDTPAELSQTILMPAAGRLDARGINSMLNTFDLPLPDLYQESSSTGL
                     YYSFDYNNVHFAVLNTNDQKADGTLSDAQIKWLKEDLEETNKPWKIVMMNKSPYSSGP
                     HIGDPETIALRNQIGDLLYKYRVDLVLQGNDGVYMRSQPIRANRDAGSDAHIITNSEG
                     KMYTSVLNP"
BASE COUNT         2408 a         2522 c         2353 g         2526 t
ORIGIN      
        1 tatattttct gacgatactc ctcggcggcc cgatatgcaa acttttcgga gccgactccg
       61 agacaaccga ccttgcggta aaggcgatac ccttaaacag ctggtcgttt atcttcgccg
      121 gacttaacac gattatctcc gcatatctct actcaacaaa gagaacaaaa gaatcggcgg
      181 cggtaaatgt ccttaggggc tttatcctct caccgatttg cgtgtttctc ctttcaatgg
      241 ccttcaaaga cgcgataatc tggcacacca taggaatcgc cgaggctctg accttcatcg
      301 ccgccctctt gatagtaaag aactccgaaa aacacggcgt ggattttaac aaaaaataaa
      361 gccccgccgg cggcagagct tttattgcac acagcccgcg gccttgctgt gatggaagat
      421 aaaacattgt ttccataaaa atgagcaatt cctttaaaaa taaaaccccc tgctcggcag
      481 ggggtgtttt attaaagagc tatttcctta ttggtgacta tgaaaatcat tctttcctga
      541 attgaggggc cgccgtgcat ggtggatata ccgccgtggt cgctggtaat aactatgagc
      601 caatcctcct gggcataggt ttcccttgcc tgaattgcgc aaatcagctc ataggccgct
      661 ctctcggact cggcgtatcc ctttctgtac ttgggattgt taacgctaaa gcccgtgctg
      721 tgtcccgcct tatcgagatg ctcgaaaatg cccattataa agtcggggca gtcggcttct
      781 gctaccgcgg ccatgagaga cgcgtgggtg ccttcatcgt cagggttgca gttaaagacc
      841 acattaagac ccttttcctc gcagtactgc ttttccagct tataggtgga gtcgtcgttt
      901 acaaaatgtc ctctccacga aacctggaac atggtagagt cagccttgcc gctctccaca
      961 agggaggtaa gaagggttag gggctcaacc gcctttgtca tgccgttgtt gataatgccg
     1021 tgctcatttg cccatttgcc tgtgagaatc gagcaccagc cgggggcggt ggatgtaaac
     1081 tgcctgttaa tcttcgggaa atttaccccg cctgtgtaag cgagataagc cttgccgccc
     1141 tgctccaaaa gaagcttgga gccgccgacc tcaagggagg agatgtccag gacatctgct
     1201 ctgccgccgt caaatccgat gatgattgcc ttcttctcgg tcttgccgtc gggcaggggg
     1261 gcatcgaaat gctctgaaac aaggtcatag actattatct ggggcagagc ggtttcgatg
     1321 gtgttttcat aaacctgcaa attctttact ctgctttgga aatactttgc ggtgtcggtc
     1381 aaatcggcgc cgtagccgaa gattgaaaca agaagggttg aaagagccgt aatcgctgcc
     1441 gtaagcaaag ccattaaatt cataaatacg cacctccgaa ataaggtagt ttccggcaag
     1501 gacatcgtat cacaaaatat agccttacac aagtcgaaaa aaataaaacc accctgcatt
     1561 aacagagtgg tttggtgcgg ataagaggac ttgaacctcc accgagttgc cccgaccaga
     1621 acctgaatct ggcgcgtctg ccaattccgc catatccgca tttatcggcg ggctgtcccg
     1681 ccgataacat attatatatt aaacttttta ttttgtaaat cccttatttt tatgttttaa
     1741 agacaaaagc gctcaaaatt ttgttaaatg gcgcaatact tagctcccgc cgcaaaaaaa
     1801 agacccttcg attgaagggt ctttggtatt ttgattatta tgcttcctca tcgagtatgg
     1861 gccactcgaa ctcgtacttt cttacattct caaccgtgaa ggagatgttg acttcgcctg
     1921 cgggagcgag cttgccaaga agctctgcct tagcggtgac atcaacattc tttctgtact
     1981 cgatgctgat tatctcgtcc gtgagcctgt ttacaaccgc gtagatggag cagttcttgt
     2041 aaacggcgtc atagtccttc acatcggcat agcccttcat tgcgtccttg ttgagggcgt
     2101 tgaagatatc gtccttagtc ctcaggtcaa aggccttgcc gattctgctg gagtttgcgg
     2161 tggggttgta ctcgtcgttg aagcggattg tgattttgta gaggttctca tcgctgttgt
     2221 cctcgatgac taccgcatag gaggtatcga aagcggagag tccgcccgcg ccgtagagga
     2281 tatcctcata agcggtcgcg ttgccgtttt tgtcgggaac ggttgtggcg ctcttctgct
     2341 tgggaaggtt ctggattatg tagttcttaa caataggagc tatgttgtta aggtcgttta
     2401 taagttcctc ggcgtccttt tcaagggtga cggtctcgtc ctcggcgccg gaaagcttag
     2461 cggatgtaat ctttatggac ttatcgctga cctttataga ctcctcccag cttatatctg
     2521 gcctttgctc tttggggagg cttaccgcct tttcaagcag gctgttgaaa tatgctactg
     2581 cctttgcggt atccgcgggt ttttccgtaa ggcttgtgga gtactcggtt gtggtcgtgc
     2641 tctcttcgcc tattgtgaac atattgcatc cggcaaagct gagcagtata agcagtacgg
     2701 caagcatagc cgatacgact ttgagcgttt tcttcataac aggataatcc cctttcttaa
     2761 ttggaaagca cattattatt ctgtttggta aatttaaact gcaaaggaaa gtatatcata
     2821 taacctcagt ttattacaag cctttggcgg aaaatattag tttatgcctt gagaccctgc
     2881 tcgtcaaaga ggaataccgt gttaagtacg atttcaaggc ccttttcgat tgtcttgggg
     2941 tcaaggttgt cgtgggagtc aagacgggta tgatagtacc tcggcgggcc ggggtccatt
     3001 gcggcaaggg ttgctgcgtg gtagccctgt cttgagattt cggccgcgtc ggacgcgccc
     3061 gcataaacgc ttgcgtattt aaggtccatg ccgcagagct tgcctgcctc cttcataaga
     3121 gcgcagacct tctcggagtt cctgacaagt cccgacatat cctttgagta tatgttaata
     3181 tgctcatagt ctcttaaggt ttcaacgcct acaaagatgg tttccacatt cttgtctttg
     3241 agcatatctt cgtgctgctt agcgaatgcc tttgcgcctc tgagacccga ctcctcggag
     3301 cctgtaagca gggcgatgac ctcggtgttc tcaaagcgga tgtcgttatc ggcaaggaac
     3361 ctgataacag cggcgctgca aaggctgccc gtaaggttgt cgttcgcgcc gggcgccatg
     3421 accttttcgt taagcatgaa gtaaagacct ataaagaagg gagtaaagca tacggagatg
     3481 ataaacatta caaagggata gccctttgcg ggagcctctg tgatatagcc ctttgcgaga
     3541 gctaccatgg ttgttataaa gaagaaggca aaaccgacgg cggcgccgcc cagcataaag
     3601 aacagaagga acttgccgcc cttgtgggtg tatctccact cataggagct gtccatatga
     3661 cccgaaacga taatccttct ctttgtctcg ccctttgcct ttctgaccgc cacaaggttg
     3721 tgggaggttg cgggcttgta aagagcgtcc gtaaactcgc ggtagaaaag gaactcgcca
     3781 aggagaatcg ctgctgcggc tacaagaagc atgaatgtga gtatcggcat gcctgcaagg
     3841 aaaacaataa gaccgaaaat caaaagaatg gatacaatct taaaccagcc cataaaagcc
     3901 ttggggcgaa ccttaaaagg ctctatggtc acggagtcgg caaccttttc aaggtcgctc
     3961 ttcataaact cctgagcctt cagctcctgc tcgctgcccg cggctctggg gccgatttcg
     4021 gtgcagattt tcttaatgcc cctgattatg tagttggtgt attcccttag tttggaatta
     4081 aaggcaggta cagattcgct tgctttcatt atctctcctc cgtatcagaa aactttagat
     4141 agcccaaagt cgtatttttg tagcatttta atataaatta aagttattgt cgatacaaaa
     4201 tcctatattt tacaaaatag acataagttt ttttgtttaa ttaaggcaaa aacagcccgg
     4261 agggcggttt aaacatccag accgaaatgc tcctttaaat acacggggat ttcgctgtcc
     4321 gaaagctcct ttgcggaaat cagctcggag ccttcaaaaa tcttaaatgt gttcttgtca
     4381 agggtcttgc gtcccgtcag agtcttaatg gctatcatgt agtccttgtt gaatatggaa
     4441 tcctcgtgct tttcgcagta gaaagaaggc tgaacgaagt cgatggggtg ctgaacctcc
     4501 tcggtaaatg agaagacatc gccccagccc ttatttgttt tctcggtgag aacccagccg
     4561 aagaagtccc ttttttcaaa gcggtactcc tcgccgaact gcttttggac aagcccctct
     4621 tttaaaagca gcggacagcg gggcgccttt gcgcccacgc ccacatcgca catataaacg
     4681 ccgtctgcgg cttctacttt caaaactcga tggcggcgca tgggaacgcc gacttcgcct
     4741 cttaaaaagc ggcccatgta ctcggtgacg ccaaagccca gagcgcgaag gagccaggct
     4801 aaaatgccgt tgagctcaaa gcagtatcct cccctgcgcc ttaaaaccac cttgtcaaaa
     4861 agggagtcgt cgtcaagttt caggggagta ccccttaaaa tgtctatgtt ctcatagggc
     4921 acggccgtga catgggcaag ctgaagtttc cgcaaatatt cgccgttcag ctctgcgttt
     4981 tcatcgtatt caagcccgat tcgctcaaag tatttaagta tcttttcttt ttcagtcata
     5041 ttaacaccgt cttttatgta ttgccccgtg gggctttttt atttttagga tgcggggcgc
     5101 gcctcttggc gggcgggcag taaaaataaa gcgctcccct gccgccccct ccctgccgct
     5161 cgcgcgggcg gaggtgatgc ccctgcggcg ggcggacaaa aaaactctcg gccgccgcct
     5221 ttccctctga gtgggtgggg taaccctgct gacgggtcgt cgtcttctcc cttcctgcgg
     5281 gttattcgga tatcctgcgg cggagcatat cgccttttct tatgctctcg ccaagattta
     5341 caagaacctt ctcctgaaca agatgggcgg tttctctctc gctgccctcc atatccctta
     5401 tttccttaac cgtaaccttc ttattaaggc tgtcggggga gagtagctcc aaaacatctc
     5461 cctttttaaa gaaattcctc tgctccaaaa gggtaaagcc ctcgctgttt gtatcctcaa
     5521 gggctattgc tataaagtcg caggttcttc tgtactcgcc gtcgttataa tggttgtgta
     5581 tcagttcccc gaaataaaag ccgccggagt agggtctgtg gcttatggtg tcaagctgga
     5641 gccttaaatc ctttatatta taggtcttgt cgatagcttt gcggtaggag tcggtgacag
     5701 tcgccacata gtagggagtt ttcatcctgc cctctatttt gaaggagtcc acccccgctt
     5761 ttctcagctc gtcaaggaac tcaatggcgc aaaggtcgtg ggagcttaaa atcgcgctga
     5821 actccccttc ctcctcaatg tcaaagctct ggtcgggtct tttcttttcc acaagggcgt
     5881 actgccagcg gcacggctgg gcgcactctc ccttgttgcc gctgcgtccc gtaaggaacg
     5941 agctaataag gcatctgccc gaataggaca tgcacatggc tccgtggacg aaggcctcaa
     6001 gctgcaaatc cttcggggtc tttgccctca gctccgctat gctctcaatg gacagctccc
     6061 tcgcgagaat cacccttttt gcgcccatgt cataatagac cctcgccgcc atgtagttca
     6121 tgcagtttgc ctgggtgctg acatgcacct ctatttcggg cacttttctt tttatttcgg
     6181 ccaatactcc tatgtcggag acaatcacgg cgtccacgcc gatttcataa agctcccttg
     6241 cgtactcgcc cgccttttct atgtcgctgt tttcggcaaa gcagtttagg gttatataaa
     6301 tccttttgcc cttttcgtga acacgcctta ccgcctcttt taaggcagcg tcggacatgg
     6361 cggctttggc agctctgagc tgcattatcc tgccgccggc gtaaaccgcg tcagcgccga
     6421 aatgcagcgc cgcatctata cattccaaat ctcccgcggg agacaaaagc tcagcgtttt
     6481 tcatagttta ccttcttttt gcgtttcttt atgaattcta tcaaatcacg gctgacagtg
     6541 tcaaggaggc agcctttcag ccttgatgcg acgcctttcc agcaaaattc gccttttatt
     6601 ggtatttgct atttacataa aaattatgtt atattataat aaaacagaat ctatattttt
     6661 ggaacgattc agtcaaaagt gggaggaatt aaaatgaaat ttaagccaaa gggcaagaca
     6721 aagctgatgt ccgttatcct cgcggcggct atgcttttgt cggcagtccc ctcaggtctt
     6781 attttgccgg cgctggcttc tgacactccc gatgtttcgg ttgcggtctt tacagacatt
     6841 ggatatatga cccccgaaga gcaaggggtc ggcagtgagg atttcgaagc tttcagaagg
     6901 aattccgaca ccctctatga gtacatggag ggcatactgg actctgccct tgcctccgtt
     6961 aaggacaagg ctttaaaggg cgagctgcgg tatcttattg taaacggctc tctcacagcc
     7021 accggctccg aaaaatctca tcttgctctt gcggcaaagc tcaaagagct ggaggacagc
     7081 acagaaggcg cccttcaggt cattgcgatg gcaggcgagg gcgacatcaa cgcttcctct
     7141 ttcagctttg aaaacggagt taaggagagc gcgcccggaa tcactccctc tcgctttaag
     7201 gaaatatacg cggatttggg ctacgacctt gctgacggtc tttatgacgc ggctgtatca
     7261 aaggcgggcg gacttacata cacagtttcg ctgaacggcg acattcgcct tatcatggca
     7321 gatgtttcaa agtacagccc cgacattaca agcacaggca cagccgccgc tgaaaagggc
     7381 ggcgtaatcg cgccggagct tcttgattac ataaaggccc aggctcttga tgccaaagta
     7441 aacggacaag tccccgtgct tgtttccgac tttgccgtcg tgcccgtaaa ccatgtaata
     7501 gagagcttga attcctccga ctttgtaagg aactaccaca aaatcgccga ggagcttgcc
     7561 gacgccggaa ttcacttcca catcggcggc aagcccggct ataacgatat agcaagcata
     7621 gtatccgacg agggcgagac gatttacggc attctctcct ccgcccttac atccttcccc
     7681 gcaacataca gaattatagg gcttacaaag ggcgagggac aaaccctctc agcctgcgcg
     7741 gagcttgttg actgcgacga tgttcagccc gtggtttata aaggaaacaa tgttcagaag
     7801 cctttcaaaa agaccactgc tttgaacgct tcctttggcg gcagctccca ggcatatgtt
     7861 gagaagttta taagaaatct tgccgatgac ttccttgaca aggtaaaggc ggcaggcgga
     7921 tttataaagt actatgagga cattaagggc attaacatcc gctttatggt ggacagcctg
     7981 ttctttggcg gcatcagcat cgacggcacc cccgttctct caggtcagaa tgtggagagc
     8041 ttccttaacg ccatcgacaa tcagctgatg aaacgctacc ttaacgaccg ctcatacacc
     8101 tatgagctgg taaagaaggt ttcctcaaga atcgccggtc tccccgcgtc aaacctcccc
     8161 tgcgacaagt tctataaaga ctatcgtata ggcagcccct acagagcagg cacattcggc
     8221 gacgcgcttg tttccgtaat gctctacatg actctgggca acgaggataa gagtgacgac
     8281 gcctttataa acgatgttat aaacaacctc tacaacacct ctctctccga ggatgtttac
     8341 agaatcgccc ttgaggtgct gtttgacgat attatcaaag acgctctgaa gcacacaaag
     8401 attgacatca gctcctattt tgagggcgag gccaagaaaa tcgccaacta tatacagctt
     8461 ggctttgagt ttttaatgtt ccttatcaga ggcgacaaca gttacttcaa tctccttgag
     8521 attatcctga aaaactccaa cacaaaatac ggtgaatcct ttgaggactt aaagaattac
     8581 attataaacg actccatcgc gaaagaggat tttgaaatag tgggcatcag cgcggcagag
     8641 cttatagacg ctgccgttac ggactctaac cccgccgaaa agagcgacca cggcgtatgc
     8701 ttttcttaca acggcaaggt taaagtcgtt cccaccaaag aagagctgcg tctgccctca
     8761 atgaaggccg taaccttcgg cgagaataca cagacaagcg ttaatataag ctggtacacc
     8821 aagctctccg tcaccggaac ggatattgag atttatcccg caaacggaaa caatcctccc
     8881 atgttcacag gaacgcccac aacaggcgcg ggaatcagga aattcacaca ggttgtaaag
     8941 aggagctatc ccgcaggcag cttcggtatc tttgacttcg gcagctccga agttgaaaca
     9001 aggcgccatg tgattcagct cacgggtctc gagaagggca agaaatactt ctaccgcgtg
     9061 ggcgacgcct ccagaggctg gtggagcgaa atcggctcct tcacaacggg agccggagaa
     9121 ggcaacttca ccttcctcta catgagcgaa tccggcggca agaccaaggc tcagtacgag
     9181 attttcgcca acaccgttaa agaggctctg aaggcatatc ccaagacctc cttcataatg
     9241 catgcgggca gccttacagc aaacggcgcc aacacaaggc actggcagtg ggctcttgac
     9301 acacccgcag agctctctca gacaatcctc atgcccgccg cgggcagact cgacgcaagg
     9361 ggcatcaaca gcatgctcaa tacctttgac ctgcccctgc ccgacctcta tcaggagagc
     9421 tccagcacag gtctttacta ctcttttgac tacaacaatg tccacttcgc ggttctgaac
     9481 accaacgacc agaaggccga cggaacctta agcgacgctc agattaagtg gcttaaagag
     9541 gaccttgagg agacaaacaa gccctggaag atagtaatga tgaataagtc accctactcc
     9601 agcggcccgc atatcggcga ccccgagaca atagctctca ggaaccagat aggtgacctc
     9661 ctctacaagt acagagtgga ccttgttctt cagggcaacg acggagtata tatgcgctca
     9721 cagcccatca gggccaacag ggacgcaggc tccgacgctc acatcattac aaattccgaa
     9781 ggcaagatgt acacctcggt acttaaccc
//
LOCUS       DAONVQ010000075         9720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5377_length_9720_cov_4.662183, whole genome shotgun sequence.
ACCESSION   DAONVQ010000075 DAONVQ010000000
VERSION     DAONVQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5377_length_9720_cov_4.662183"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLD98_05840"
     CDS             <1..631
                     /locus_tag="PLD98_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQA05804.1"
                     /translation="GESEGLLAAVLPDDATNKQVVWTSSNSSVASVSQNGAVTALKVG
                     TAVITVTTADGGFTATCAVTVVPNKIELKPGLDYKLNESGTIVYNIKPETKVSDLVKN
                     FAGSGTIEVRNKDGVLLSGDKFVGTGAIINLIAGGRVVDTLIVAVLGDVNGDGKVLAN
                     DARLVLRHVAKLGTLTELQMLAGDTDGNKKIEATDARIILRVAAKLHKF"
     gene            complement(734..810)
                     /locus_tag="PLD98_05845"
     tRNA            complement(734..810)
                     /locus_tag="PLD98_05845"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(774..776),aa:Ala,seq:ggc)
     gene            1205..2116
                     /gene="pyrB"
                     /locus_tag="PLD98_05850"
     CDS             1205..2116
                     /gene="pyrB"
                     /locus_tag="PLD98_05850"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HQA05805.1"
                     /translation="MRHLISITDLTKSEIDELIELGLKIIENPKDYSEVCRGKKLATL
                     FFEPSTRTRLSFEAAMLELGGQVLGFSSADTSSAAKGESVTDTVRTVACYADIIAMRH
                     NKEGAPLAAAMKSTVPIINAGDGGHQHPTQTLTDLITIKREKGYIGDITVGFCGDLQF
                     GRTVHSLIKALLMYSGIKFVLISPNELRVPGYIREALRHHNAEFVEIERLEEVIDELD
                     VLYMTRVQRERFFNEADYVRLKDSYILTLDKLKTAKQDLSILHPLPRVNEIAVEVDDD
                     PRACYFKQVLNGKYIRMALIMKLLELV"
     gene            2179..2607
                     /locus_tag="PLD98_05855"
     CDS             2179..2607
                     /locus_tag="PLD98_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HQA05806.1"
                     /translation="MYVDGIDCGFVLDHITAGKGMEIYKALELDKLDCSVAVIQNVKS
                     SKYGRKDIIKIDSMIDLNFDNLGYIDKNITVNTIKDGKLIDKRHISPPTILVNILRCK
                     NPRCITSTERGVDHIFKMSEGEDGVYRCIYCESDHKEKTL"
     gene            2610..3275
                     /gene="deoC"
                     /locus_tag="PLD98_05860"
     CDS             2610..3275
                     /gene="deoC"
                     /locus_tag="PLD98_05860"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQA05807.1"
                     /translation="MDIKEILSRVDHTILKPETVWEDVDRVCAEAVKYSVASVCIPPS
                     FVRRARYHYPELNICTVVGFPNGYNSPFAKEFETILAVKDGADEIDMVINIGLVKEGR
                     YDEVLSEILLIREACHGKILKVIIETCLLTEEEKIEMCKVVTKSGADYIKTSTGFGSG
                     GATRRDILLFKEHIGDSVKIKAAGGIKSLEDAKDFIVLGADRLGTSSLVKLAEGLIVD
                     SSY"
     gene            3297..3635
                     /locus_tag="PLD98_05865"
     CDS             3297..3635
                     /locus_tag="PLD98_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HQA05808.1"
                     /translation="MAKYEATLRGDFNIILKDIEDSVMRKSLSASFEDGSFYQDGDTA
                     FAFRVYERYSYFGNNRVSLSILLFKKGDDIKFSAITSGGSQAVFFKINTVGEQNFLST
                     IIDTVEKYRI"
     gene            3739..4371
                     /locus_tag="PLD98_05870"
     CDS             3739..4371
                     /locus_tag="PLD98_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HQA05809.1"
                     /translation="MLKHFVKSFLGGFVIAIGAVACLSVENKALGAFLFTLGVFSVSS
                     FGFCLFTGKVCFIPNKQSSFIKDIILTYLGNFIGTVSLGLVLPFTKLSILVPKAQDIA
                     ALKLSDTLFSAFVMSVLCGMMICISVLGFKTIKDGTGKYIALFLPIMVYILAGFENSI
                     ANLFYYTFAGAWGSPALIHSAVVALGNLIGGTLIPFAAKYTDNIKLGCEE"
     gene            4569..5378
                     /locus_tag="PLD98_05875"
     CDS             4569..5378
                     /locus_tag="PLD98_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05810.1"
                     /translation="MYYYYYKDNVPLKKKDNSRKGGKDDCPYCSKDFDYSKLSIPCSC
                     PTCRQAQADEREGGRWSNHGFEGERNGRGNSGNNQGFYGEYEFPMNDDGPHTFELEFD
                     EDFRPQHFSGNKFEGPFRDDYRDYGSDYYAYGRPDWGKDKKNDKSDPNLKLYLNINIC
                     KKCGKCSCDVKPFPPHKCKEPCEFCGCEPCRCDVKPCPPPPPPHPPLPPCDKRPIDCC
                     PEPVCCDDSVGPPPPHGNFCHCCGSEPCRCNRFPGYPEMESEHFDYYWDEQ"
     gene            5566..6459
                     /locus_tag="PLD98_05880"
     CDS             5566..6459
                     /locus_tag="PLD98_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HQA05811.1"
                     /translation="MNLKKITALMIILLLTFSAFGCGKQTPKSGEGNLTVYTSFYVMY
                     DFAKKIGGDKAEVINMVPAGTEPHDWEPSAADIAGLERADIFIYNGKGMEHWTEDVLK
                     TIGSNKLITVEASAGIEDVQGDHSDPHVWLSPMNAKKQMENIKDAFAEADPENAEYYE
                     ANFSKYSAELDALDAEFKSVIEPLPRRDIVVSHEAFGYLCKAYGLRQIAVEGLSPDSE
                     PDAARVSDIIRFIKENNVKVIFFESLSSAKVAQTISANSGAKAEVLNSLEGLTDEEIK
                     NGEDYFSVMRKNLAALKNALE"
     gene            6546..7322
                     /locus_tag="PLD98_05885"
     CDS             6546..7322
                     /locus_tag="PLD98_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05812.1"
                     /translation="MNLIDVKDLSFSYENEPLFSDISFSVKEGDCTAVIGSNGAGKST
                     LMRLILGELQPDKGSVSLFGQDVRQFKDWHKVGYVKQSGLQDAINFPATAKEIVLANL
                     YSQIGMFRFPKKEHKEKAMAALESVGMADYADKTLGEMSGGQRQRVALAMLLVADPML
                     LLLDEPVSGVDSKNVAAIYEILTRLNEEQGKTILMITHDTVRVSGYVNRMLCIEDGCI
                     IEEGVGHPDVLAHYHQHHFIRTESIKGRTADTDCCQNHEN"
     gene            7333..8160
                     /locus_tag="PLD98_05890"
     CDS             7333..8160
                     /locus_tag="PLD98_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HQA05813.1"
                     /translation="MAIFEFEFMQRAFLVSILLALIIPCIGVIVVLKRLSMIGDSLAH
                     TSLAGVAAGLILNINPVAGAITACIAASLGIEFIRKRLPKYAEMSIAIIMSAGIGLAG
                     VLSGFVKTANFNSFLFGSIVAISDFELRLVVMVSLLVLISFVLLYKELFFISLDERLA
                     RLAGVPVWAVNFIFTILTAVTVSVAARTVGALIVSSLMVIPTACAMQYAKSYKRTVIY
                     SICFAVLFMISGLFISYYLRLKPGGTIVLIGVVCLLLSFAVKGLPRIFKKHRSAKAR"
     gene            8186..8608
                     /locus_tag="PLD98_05895"
     CDS             8186..8608
                     /locus_tag="PLD98_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQA05814.1"
                     /translation="MESSIKWPENFKRTKAREAILSVLENSPEPLSAADIYSKIDSRG
                     EDVWLSTIYRTLEQLVDRGVALKARILPGGKALYELNRESHKHYAVCLACNKVIHLEE
                     CPIENLNLSIDDSGFKVTGHNLEVFGYCVDCKDKDKEK"
     gene            complement(8638..>9720)
                     /locus_tag="PLD98_05900"
     CDS             complement(8638..>9720)
                     /locus_tag="PLD98_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05815.1"
                     /translation="MTLSFCFILKKGYKNMFKSLKDASLIADEILVADFSKDNIQNIM
                     QSNTGLADDALVYGFDPSIDYSFAIDYLSKSASGDWILLLFGDEDLSVPNPDEVKKEL
                     ENTDAEAFSVRIVHLLGRAPHSPIRAFKTYSPRLFRKGQNFRFCGKIPEMLGCNISEN
                     RPQVMKDITIKNYAFTFEGIEPSCADILSENGSMFCCKAFESFCAGEYDKALSLLNQA
                     EKNYKEKGEKPPAVLYKLRFETMLNMGEIAKVYKEIRNAIRLYPDYVDLHFYKALSLF
                     ANNRFKEAAEGFSYCLLLGEGNKDYIISCGYGACLALYFLGISYTKMGEEKHFEELFN
                     QASRVCPDPKALEAEARKIPGIKLQQ"
BASE COUNT         2737 a         2167 c         2322 g         2494 t
ORIGIN      
        1 cggcgagagc gagggtttgt tagctgctgt ccttcctgat gatgccacaa ataagcaggt
       61 tgtctggact tcgagcaaca gcagtgttgc gtccgtaagc caaaatggag cagtaacggc
      121 actgaaggtc ggaacggctg tcattaccgt caccaccgca gacggcggat ttaccgcaac
      181 ctgcgccgtc acagtcgttc cgaacaaaat tgagcttaaa ccaggcttgg actataagct
      241 taatgaaagc ggaacaattg tttataacat caagcccgaa acaaaagtaa gcgaccttgt
      301 taagaacttt gcgggcagcg gaactattga agtaagaaac aaagacggag tgctgctgtc
      361 aggtgataag tttgtaggta caggagcaat aataaatctt atcgcgggcg gcagggtagt
      421 cgatacacta atagtcgcgg tacttggcga tgttaacgga gacggaaagg tgctcgcgaa
      481 cgacgcaaga ctggtactaa gacatgtggc taaactcggt actcttaccg agcttcaaat
      541 gctcgcggga gacacagacg gcaataagaa aattgaggct acagacgcgc gaatcatcct
      601 ccgtgtagcc gcaaaactgc ataagtttta attcacgcac aaaaacaggg tccccaaaaa
      661 ggaccctgtt tttatttaag acttaaatga cactttgcat aataaaaagc cgacagtcta
      721 aactgtcggc tcttggtgga gacgaagggg atcgaaccct cgacctcttg aatgccattc
      781 aagcgctctc ccagactgag ctacgccccc atagcgcatc gcgcagtgat tatattagca
      841 tacagactct ttttagtcaa tactttattt tctggtttta cggcataaaa actttggcag
      901 cgggagaaga aaattattga aataaaaggt ttaggaaaag acaagttaaa accaaaacaa
      961 tgcttgacta cactgtgacg agatagtata atacaaaaaa cagacggtga ggcaggtgga
     1021 agcgcatgag attgggggac aaaatcttgt tcgatcatcc tgcgttttcc gattttaaat
     1081 cacccgtctt gagctcaccg tcaccccaac acggttcatt tcccgttttt aatttaataa
     1141 aagaagaagt taaggatttt tgattctatt caaaaatcct ttttttttgt gttcaggagg
     1201 ttttatgagg cacttaatca gtatcacgga tttaacaaag tctgaaattg atgagcttat
     1261 tgaacttggg ctgaaaataa ttgagaatcc caaggattac agcgaggtat gccgcggcaa
     1321 gaaactggca accttgttct ttgagcccag cacaaggacc cgccttagct ttgaggctgc
     1381 tatgcttgag cttggcggtc aggtcttggg attttcttca gcggatacaa gctccgccgc
     1441 taagggcgag agcgtgacag acaccgtaag aacagtcgcc tgctatgcgg acatcatagc
     1501 catgcgtcac aataaggagg gcgcgcccct tgccgccgca atgaagtcca cggtaccgat
     1561 aatcaacgcg ggcgacggcg gtcatcagca cccgacccag accctcaccg accttatcac
     1621 aatcaagagg gaaaagggat acatcggaga tataaccgtc ggcttctgcg gtgacctgca
     1681 gttcggcaga acggttcact ctcttataaa ggcgctgctt atgtacagcg gaattaagtt
     1741 tgttctgatt tctcccaatg agctgagagt cccgggatac atcagagagg ccctcaggca
     1801 ccataatgcc gaattcgtag agattgaaag gctcgaggag gttatagacg aactcgatgt
     1861 tctttacatg accagagttc agcgcgaaag attcttcaac gaggccgatt atgtaaggct
     1921 gaaggacagc tacatcttaa cactggataa acttaagacc gcaaagcagg acctttcgat
     1981 tctgcatccg ctgccgaggg tcaacgaaat agcggttgaa gtggacgacg acccgagagc
     2041 ctgctatttc aaacaggttc ttaacggcaa atatatcaga atggcattaa tcatgaaact
     2101 tctggagtta gtatgagcgc atatacggga ttagaaataa taaacataaa tacaagcaaa
     2161 aacgaggtgg ggacagaaat gtatgtagac ggcattgact gcggctttgt gctggaccac
     2221 attaccgcag gcaagggcat ggagatttac aaggctcttg agcttgacaa gctggactgc
     2281 tccgtagcgg taattcaaaa tgtaaagagt tcaaaatacg gacgcaagga cattataaag
     2341 attgacagca tgattgacct taattttgat aatctgggct atatcgacaa gaatattacc
     2401 gtcaacacca taaaagacgg aaagctaatt gacaaaaggc atatatctcc gcccacgatt
     2461 cttgtaaata ttctgcggtg caagaatccc cgctgcatta catccaccga gaggggagta
     2521 gaccatattt tcaagatgag cgagggcgaa gacggtgttt accgctgcat ttactgcgaa
     2581 tctgaccata aggagaaaac attataataa tggatattaa agaaatacta agtcgtgttg
     2641 accatacaat attaaaaccc gaaaccgtct gggaagatgt ggacagggtg tgcgccgagg
     2701 cggttaagta ctctgtcgcc agcgtctgca tcccgccctc gtttgtaagg cgggcaaggt
     2761 atcattatcc cgagcttaac atctgcactg ttgttggctt tccgaacggc tataattctc
     2821 ctttcgcaaa ggagtttgaa acaatcctgg ctgtcaaaga cggcgccgat gaaattgata
     2881 tggttataaa catcgggctt gtcaaagaag gcagatacga tgaggttctc tcggaaatcc
     2941 tgcttatcag agaagcctgc catggcaaga tactaaaggt tataatagaa acctgccttt
     3001 tgaccgagga agaaaaaatc gagatgtgca aagtggtcac aaagtcggga gccgattata
     3061 tcaaaacctc aacgggattt ggatcgggcg gagccacaag gcgtgacata ctgctgttta
     3121 aagaacacat cggcgacagc gttaagataa aggctgcggg cggaataaaa agccttgaag
     3181 acgcaaagga ttttatcgtt ctcggagcag acagactcgg caccagctct cttgtaaagc
     3241 tggctgaagg tcttattgtc gacagctctt attaatttcg aatcgaggtt tttattatgg
     3301 caaaatacga ggcaactctg aggggcgatt ttaacatcat cctcaaggat attgaagata
     3361 gcgttatgag aaagagcctc agcgccagct ttgaagacgg cagcttttat caggacggcg
     3421 acaccgcctt tgctttcaga gtttatgaaa gatacagcta ttttggaaac aacagggtga
     3481 gtcttagcat cctgctgttt aagaagggcg atgatataaa attcagcgcc ataacctcag
     3541 gcggcagcca ggcggtgttc tttaagataa acacagtggg tgagcagaat ttcctctcga
     3601 ccatcataga cacggtggaa aaatacagaa tataattaca gcccgacatc gtcgggctta
     3661 tttatgttga ctgaaaggcg caaagatggt atactcgtcg ttgctttgcg cttttcagta
     3721 ctgttttttg aggttattat gctaaaacat tttgttaagt cttttcttgg aggctttgtt
     3781 attgcaatag gggctgtagc ctgtctttcc gtggaaaaca aagccttagg cgcgtttttg
     3841 ttcacccttg gagtattttc ggtttccagc ttcggctttt gcttattcac aggcaaggtt
     3901 tgctttatcc cgaataaaca atcctccttt attaaggata ttatccttac atatttgggc
     3961 aattttatcg gcacggtatc attagggctt gttctccctt ttactaagct gagtatcctt
     4021 gttcccaaag ctcaggatat tgcggctctt aaactctccg atacactttt cagcgccttt
     4081 gtaatgtccg tgctctgcgg aatgatgatt tgcatttctg ttttaggctt caaaaccata
     4141 aaagacggca cgggcaaata tatcgcgctg ttcctgccga ttatggtata tatccttgcc
     4201 ggctttgaga acagcatagc aaatctgttt tactatacct ttgcaggcgc gtggggcagc
     4261 ccggcgctga ttcattcggc agtcgtcgcc ctcggcaacc taatcggagg aactctgata
     4321 ccctttgcgg caaagtacac cgacaacata aaactcggct gcgaagaata aaaacacgac
     4381 cgcttaaggc ggtctttttt tatctccaaa tgagttttta tgaaccataa ccaagataaa
     4441 gcatagtatg tactgagctg ctttggcttg accgacaggc ggtcaaagcg gaaattgtag
     4501 gctgacttta aaggcaaatc ggtcagtcgt atcctcaaaa tcagaaaatg ataaagggag
     4561 atttcaaaat gtattactac tactataaag acaatgtgcc gctcaaaaag aaagataatt
     4621 cccgaaaagg cggcaaagac gattgtccgt actgttcaaa agattttgat tatagtaaat
     4681 taagcatccc gtgttcctgc cccacatgca ggcaggctca ggcagatgag agagaaggcg
     4741 gcagatggtc aaaccacggc tttgaaggtg agagaaacgg cagaggaaat tcgggaaaca
     4801 atcagggttt ctacggtgaa tatgaatttc cgatgaatga tgacggcccc cacacttttg
     4861 agcttgaatt cgatgaagac ttcaggcctc agcatttctc gggaaataaa ttcgagggtc
     4921 ccttcaggga tgattacaga gactacggtt ccgattatta cgcatacggc agacccgatt
     4981 ggggtaaaga caaaaagaat gataaatccg atcccaacct taaactctat cttaatataa
     5041 acatctgcaa aaaatgcgga aaatgctcct gcgatgtaaa gcccttcccg cctcataagt
     5101 gtaaggagcc ttgcgagttt tgcggatgcg agccctgccg ttgcgatgta aagccttgcc
     5161 ctccgcctcc tccgccgcat cctcctcttc ctccctgcga taaaagaccc attgactgct
     5221 gcccggagcc tgtttgctgt gacgatagcg taggtcctcc gccgcctcac ggcaacttct
     5281 gccactgctg cggcagtgag ccttgccgct gcaacagatt ccccggctat ccggaaatgg
     5341 aaagcgagca ttttgattac tattgggatg agcagtagct ttattaaacg gacagattag
     5401 cgtgattttg ttatttcgag ctccatatct gcggatatgg ggctctgttt tttatatttc
     5461 ttgacaataa taagtaaaaa gactatactc taactgcaaa tgcgaatcat gtgcaattag
     5521 aaaatggact gtaaaagtat tattccgctt gagaggaata tggatatgaa tttaaaaaag
     5581 attaccgctt taatgattat acttctgctg acatttagcg ccttcggctg cggcaaacaa
     5641 acgccaaaaa gcggcgaagg gaatcttacc gtatacacga gcttttatgt aatgtatgac
     5701 tttgctaaaa agattggcgg agacaaggcc gaggtcataa atatggttcc cgcgggcaca
     5761 gagcctcacg actgggagcc gagcgcagcc gatattgcgg gactcgaaag agcggatatt
     5821 ttcatttaca acggcaaggg gatggagcat tggacggagg atgtccttaa aaccatcggc
     5881 agcaataagc tgattactgt tgaagcatcc gcaggtatag aggatgtcca aggcgaccac
     5941 tctgaccccc atgtatggct gagtcccatg aacgcaaaaa agcagatgga gaatataaaa
     6001 gacgcttttg ccgaggctga ccccgaaaac gccgaatact acgaggctaa cttcagcaaa
     6061 tactccgccg agctggacgc gcttgacgcc gagtttaaaa gcgtaatcga accgctcccc
     6121 agacgagata tagtggtatc ccacgaggcg ttcggctatt tatgcaaggc ttacgggctc
     6181 aggcaaatcg ccgtagaggg actttccccc gattccgagc ccgacgcggc aagggtttcg
     6241 gatataatca ggtttattaa agagaataat gtaaaggtaa tcttctttga aagtctgagt
     6301 tccgcaaagg tcgcccagac catttcagca aactcgggcg cgaaagcaga agttctaaac
     6361 tcccttgaag gcttgaccga tgaggagata aaaaacggcg aggactattt ctccgttatg
     6421 cgtaaaaacc ttgccgccct caaaaacgcc cttgaatgaa ctgtaaagca atgggccttt
     6481 tcttgtaatg gcattttact tttcaggtga aacctatttt cttaatgaac ttcggaggat
     6541 aattcgtgaa tcttatagat gtaaaggatt tgagctttag ctatgaaaac gagcctcttt
     6601 tctcggacat aagtttttcc gtaaaagagg gcgattgcac tgctgtcatc ggctcaaacg
     6661 gagcgggcaa aagcacattg atgcgtctga ttttgggcga gctgcagccg gacaagggct
     6721 ctgtttccct tttcggacaa gatgtaaggc agtttaagga ctggcataag gtgggctatg
     6781 taaagcagtc gggccttcag gacgccatta acttccccgc cacagcaaaa gagatagtgc
     6841 tggctaacct ctattctcaa atcggcatgt tccgctttcc caaaaaggag cacaaggaaa
     6901 aggctatggc ggctctggaa agcgtcggca tggcggacta tgccgacaaa acactcggcg
     6961 aaatgtcggg cggtcaaagg caaagggtag ctcttgccat gctgcttgtg gctgacccca
     7021 tgctgctttt gcttgatgag cccgtcagcg gcgttgactc caaaaatgta gcggcgattt
     7081 atgagatact gacaaggctg aatgaagaac agggcaaaac aatactgatg attacccacg
     7141 atacggtcag agtttcgggc tatgtaaacc gaatgctctg cattgaggac ggctgcataa
     7201 ttgaggaggg cgtcggtcac cccgatgttt tagctcatta tcatcagcac cactttataa
     7261 gaaccgaaag cataaaaggc aggacagccg ataccgactg ctgccaaaat cacgagaatt
     7321 aggggtatat ttatggcgat atttgagttt gagtttatgc agagagcctt tcttgtgagt
     7381 atactgcttg ccctgattat accctgcatc ggcgtaattg tggtgctcaa aaggctttca
     7441 atgataggcg attccttggc gcacacatcc cttgcgggag tcgcggcggg tctgattcta
     7501 aacataaacc ccgtagcggg agccatcacc gcctgcattg ccgcttccct cggtatcgaa
     7561 tttataagga aacgcctgcc caaatacgct gagatgtcga tagccataat aatgtcagcg
     7621 ggcatcggac ttgcgggcgt gctttcgggc tttgtaaaga ccgcgaattt taacagcttc
     7681 ctttttggca gcatagtcgc aatcagcgat tttgagctaa ggcttgtagt aatggtttct
     7741 ctgctcgtac ttatttcctt tgttcttctt tacaaggagc tgtttttcat atcccttgac
     7801 gaacgccttg caaggcttgc gggcgtaccc gtctgggcgg taaacttcat attcaccata
     7861 cttacggcgg tcaccgtttc cgttgccgca agaaccgttg gagccttaat agtgtcgtcg
     7921 cttatggtta tccctacggc ctgcgcgatg cagtacgcaa agagctataa acgcaccgtc
     7981 atttattcaa tctgctttgc ggttttgttt atgatatcgg gacttttcat ctcatattac
     8041 ctgaggctaa agcccggcgg cacgatagtg cttataggcg ttgtctgcct gcttttaagc
     8101 tttgctgtaa agggcttacc gaggattttc aagaaacaca ggtccgcaaa agctcgctga
     8161 gaaaaataaa aagggaagta gcgatatgga atcaagcata aaatggcccg aaaattttaa
     8221 gcgcaccaag gcaagggagg cgattctctc cgttctagag aattcccccg aacccttgtc
     8281 cgccgccgat atttactcca aaatcgactc ccggggcgag gatgtatggc tttcgacaat
     8341 ataccgcact cttgagcagc ttgtggacag gggagttgcg ctcaaggcaa gaatcctccc
     8401 cggaggcaag gctctatatg aattaaaccg cgagagccac aagcattacg ccgtctgcct
     8461 tgcctgtaat aaggtaatac accttgagga gtgcccgata gagaacttga atctaagtat
     8521 tgatgacagc gggtttaaag ttacgggaca caatctcgag gttttcggct actgcgttga
     8581 ctgcaaagac aaagataaag aaaaataaaa aatcccttgt caaacaaggg attttattta
     8641 ttgctgaagt tttatgccgg gaatttttct tgcctccgct tccaaggcct ttgggtcggg
     8701 gcagacacgg gacgcttgat tgaaaagttc ctcaaagtgc ttttcttcgc ccatcttagt
     8761 atagctgatt cccagaaaat acagagcaag gcaggcgcca tacccgcagg atatgatgta
     8821 atctttgttc ccttcaccaa gcagcaggca gtacgaaaag ccctccgcag cctctttaaa
     8881 cctgttgttt gcaaataggc tcagcgcctt gtaaaaatgc aaatccacat aatcggggta
     8941 aagccttata gcgtttctga tttccttata taccttagct atttcaccca tgttcagcat
     9001 tgtctcaaac cgcagtttat aaagaacagc gggtggcttt tcgcctttct ctttataatt
     9061 cttttccgcc tgattcaaaa ggctcaaagc cttatcgtat tctcccgcgc aaaagctctc
     9121 aaaggctttg cagcaaaaca ttgagccgtt ttcgctcagt atatcggcgc agctcggctc
     9181 tatgccctca aaggtaaaag cgtaattctt tatagtaatg tccttcatga cttgaggacg
     9241 gttttcgctt atattgcagc caagcatttc gggtattttg ccgcagaatc taaaattctg
     9301 accctttctg aaaagcctcg gagaataagt tttaaaagct cttatggggg agtggggagc
     9361 tctgcccaat aggtggacta ttctaacgga gaacgcctcc gcgtcggtgt tctccagctc
     9421 tttttttacc tcatcgggat tgggcactga taaatcctca tctccgaaaa ggagcaatat
     9481 ccagtcgccg cttgccgatt tcgacaaata atcaatggca aatgagtagt ctatggaagg
     9541 atcaaaacca taaaccaaag cgtcatccgc aaggccggta ttgctttgca ttatgttttg
     9601 tatattgtct tttgaaaaat cggcgaccag tatttcatcg gctatcaagg acgcgtcttt
     9661 taagctctta aacatattct tgtaaccttt ttttaaaata aagcaaaagc tcagcgtcat
//
LOCUS       DAONVQ010000076         9676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5406_length_9676_cov_3.833801, whole genome shotgun sequence.
ACCESSION   DAONVQ010000076 DAONVQ010000000
VERSION     DAONVQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5406_length_9676_cov_3.833801"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PLD98_05905"
     CDS             complement(<1..1176)
                     /locus_tag="PLD98_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HQA05816.1"
                     /translation="MEKILTKGKAFVDEFGRERIFNGINIVDKGKPTNGVREYAYSWE
                     DDRIKRLKALGINLVRLGFTWDVIEPQPGKYNEKYIDFLSKTLDELHENGIYAFLDLH
                     QDLYSGSKGGVGDGAPAWATLTDGYEFKDQRFVWAEGYFWGKAVHRSFDNFWNNTKVG
                     GKGLQDYYADMIKHLTERLGNKPALLGIDFLNEPFPGSVSNQVIKSLLSSAVKVAFTS
                     RNISRTKLLGDLIIKDRHKYILDNFNARVFAELVLGAAPVLNKFDVEKYNPFLNKMGA
                     AVREVNNNLILFVDNSYYCNLGIPCLAGPIMVKGEKDKLQCFAPHGYDFGVDTPLYKY
                     ASNERIKFIFDEHRRTQERLGIPVIVGEWGGSSEGNEWFFHAKYILDMFDKHKWSHTY
                     "
     gene            complement(1196..2167)
                     /locus_tag="PLD98_05910"
     CDS             complement(1196..2167)
                     /locus_tag="PLD98_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HQA05817.1"
                     /translation="MKYKVLSVLCLLALLLSLCSCDISETIDDPNDPANVKAANILGK
                     TYVSDLSKDSFVFKVLNDGSLVLSGYTGSGDTLDIPETFEGKTVVGIENKALYGNKTV
                     KRLIIPDTVKVIGNFSFMEAVNLESVKIGANVSYIGVSAFNGCSSLKTVEFKGTPETI
                     DTKAFYLCSSLEKINLPSVKTIGDWAFAKCSAAKEIILSEGTELIEKYAFLKCFAAEK
                     VVIPSSCKTIGQSAFFQNTALTDLQIAEGVETICRGAFEECTSLKYVKIPASVKVIEK
                     YAFFTCTSLESVDFLTDIKELGKDAFRDTALKTVNAPEDSPIFDYVK"
     gene            complement(2167..4506)
                     /locus_tag="PLD98_05915"
     CDS             complement(2167..4506)
                     /locus_tag="PLD98_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA05818.1"
                     /translation="MNEGILTKAKSVINYIRRYWKKPPEGKYVNLKEVINYGIGGIGV
                     QFIIYFGGLIALTSSSLLVGNTIGIQPVHLQLMSVISTIIGFGITILKSYLIDTAKFK
                     MGKFRPWLAITTIPTVIISIIFVWLPYETMSYSSKVVAVFICYNLLMLFFPFYRESYN
                     NIANVISPDSNERTDIISIASILYSLAPSIANFIVPILSKFTGGLNNITTYRFIHPSI
                     AIVGAILTSFTVFGVKERIVEAVSHERSFSFISALKAVSKNKYFWIISTAGWIGFLED
                     AVAVVLTWTFVYGYADRMGTYALATTLIGNAAFWSMLLAPIVIRLIGKRNLLIWCNIA
                     NIIFIGALLPTYKNIYILILLYYLNGFAKHFALVYDVGISADIRDYQQYISGERIDGM
                     FGTVGLIGSFLGMFTGMVLPMIYEHLGLVNNYDVLEVSSFRYEMSEVLIIAAVIGATL
                     NVIPYFFYDFTEIKHKGIISVLKIRALLEDYGNGIINNSALVEVVDIINAAKENIKKK
                     DEIIAKDYINIIKKQKTHNETEKKAKEQRLSELKLKRKENKESLKKEIEEIKAAAKTK
                     EEKKAAKAEKKKLKKDYKEQQKKIVEALYVIDEIEKFNTPRMQAKLLRARKICEAGID
                     GLYKTPDTVLEEARALPANSAEEKAIRSDAITEAKQLLSCKKLLIKKFPNGITPITDE
                     DRAAVEAMSENSFAEALKKKKAFKDLDKAEDLYKKATKALHDAQRIVREYHNQSNFDE
                     LMEKYEEAKAALELEKQQNREIEEQAAQQKETAKKGRRR"
     gene            complement(4803..9593)
                     /locus_tag="PLD98_05920"
     CDS             complement(4803..9593)
                     /locus_tag="PLD98_05920"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HQA05819.1"
                     /translation="MAGKADYSAVSQTEKCEYNLSDFIPTKDNPSPEYKLYDFMTLGP
                     FVLETDGAFETEYFYERHKVLDHDYLAESGGEASEVPYLGKQVKNKYYGPEFLTWRKG
                     IRKWGDLRFDNPYESCDEALFRTEQRNCVYYAAVYINSDKEQDAVICYINSGCLLYLN
                     GELVSNTPYGRVKGLGRIGERVLVRFKKGRNLLMFKIRVGYIADSIDISMSNCEIYPV
                     VARSGNVVITPPVTSTAYFGTKEEPRQILPLFVGALSETNCAELTLKSGDYTEIHNIE
                     PMTAGSTSLIYTSAPVKDKPFTAEISLELKEGANPSGNYSNNYELTPYNGFVGTEHIF
                     TDFHFDTTYHQEQRTYAIGAIYITMEILKEIARNPLFTATISEIDYLHPVYSLYPECR
                     KILKEAYKTGRVEADCFYNQPNELTSSGEAIVRNLIYGQLYHRDVLGRIVSVYSPGDV
                     FGHPNQMSQICRKGGCNSARWGKMIIGLDALFHHVSPDGSDILHSKGISVENAMRLGI
                     THCDRSTKVNAGFELYPKEGDTSWMSKTLNGAKFSLFSNMMDGIIEDEKETVKEKGIS
                     KIEYTSRDMTQHHAAVLLTRTDYKQANRLIENLLVTAEKFSTIAALYGAEYPEKALDK
                     AWRQLLCAQHHDSITGTNNEVSFVDLMIEYREAAELAAEIVKNASEFIASAIKRSDEA
                     FPVIIFNQHTWDRDEAIEFTLPLWAESGYALFDSKGKEHSFEIIGKTHDGKKLKAVVI
                     AKVPAFGYKTFYLKKTDKPKTVIKSKDTSIENKYFKVTVDPQRGGGIVSIYDKVNKRE
                     FIDPSTDGPANRIAVLKEIHNRMETQHEFYTTGHKLFSDEFIADVSSEKTSLYDKLIV
                     TVRLDVVARVRQEITLYKNSKKIDCKTIVEDYIDNDDMFTVTFPAALKGVKPVYEDRF
                     APHVTAKSAKKLSFQTHQFFMYSRCQVAPSVNWFDLGPTTQLKFTNSAGRLKGSLNIG
                     MTAIIRPEEKDLILSANKLLKTLSKKAIPVTPYHDRTERRATKIIHFNEDISYTDTRF
                     VLSVEGVRNLYEDELLSKLTEKQREKFQNLLNKKGVAALYIKDSDNYFKKPIDVLLIK
                     AKSVYTLNAYLDELEKAYSMGYAAEIRGAVIASELEAIDDFGLGILNKGTIACSVEGE
                     NMLNMMLFHTAEFYGNIGKVTGSTELIPENKTHVFEYAIYPHEKSYREAELFKKGMEF
                     NEPMFAVTEFETAENPFLPESKSFFKTDGTFIVTAFKAGGYPLAKMEGGIKSVFERGI
                     TVRGFESNGTDCETMFKFDFEIGKVKNTDLLDENETEVKSGKNGFTAFIPTHSIETYN
                     IAVKKPQNTISASYPLGRTREIAEPTYIRSYEHDLGSMPMGYYRLVAAISRKVEALDD
                     LNVRLGISLANNQPDKSESGVLKLILPDGFKADKTEFPYEIAPDSLLEYSVVVTKPAK
                     DAKGFIKLQFTHDGQVFEDIFEFGYFNPKVRLKIEDNKVKVAVTNDTDSHLNGELALA
                     TPFETWSLLGFNTAARGEVGPCAIPVSLKPHSKERYEFDVKFTEDDIMNSWWSAAKLM
                     VNGRIHFAYDAVKSERHNMGTHIFYDEIVDDNHSMRKLFEIQAR"
BASE COUNT         2274 a         2162 c         2339 g         2901 t
ORIGIN      
        1 ataggtgtgg ctccacttgt gcttatcgaa catatcgagg atgtattttg cgtggaagaa
       61 ccactcgttg ccctcggagg agccgcccca ctctcctact attacgggta tgccgagtct
      121 ttcctgagtt ctcctgtgct cgtcaaagat aaacttgatt ctctcgttgc tggcgtactt
      181 atacagcggt gtgtccacgc cgaaatcata gccatgggga gcgaagcact ggagcttatc
      241 cttttcgccc tttaccataa tgggccccgc aaggcagggg atgcccaaat tgcagtagta
      301 tgagttgtca acgaaaagaa tcagattgtt gttgacctct cttacagcgg cgcccatttt
      361 gttaaggaag gggttgtact tttcaacatc gaacttatta agaacgggcg ctgctcccag
      421 aaccagctct gcgaaaaccc ttgcgttaaa gttgtccaga atgtacttat gcctgtcttt
      481 tataatcaag tcgccaagaa gttttgtgcg gctgatgttc cttgaagtaa aggcaacctt
      541 tacagcggaa gaaaggaggc tctttataac ctgattgctg acgctgccgg ggaagggctc
      601 gtttaaaaag tcaattccca aaagggcggg cttattgccg agacgctcag tcaagtgctt
      661 tatcatatcg gcataatagt cctgaagtcc cttgccgcct actttagtat tgttccagaa
      721 attatcgaag gagcgatgta ccgccttgcc ccagaaatag ccttctgccc aaacaaaacg
      781 ctggtcctta aattcgtatc cgtcggtaag tgtcgcccat gcgggagcgc cgtcgcccac
      841 tccccccttg ctgcctgaat aaaggtcctg atgcaaatca aggaaggcgt agatgccgtt
      901 ttcatgcagc tcatcaaggg ttttggaaag aaaatctatg tatttttcat tgtatttgcc
      961 cggctggggc tctatcacat cccatgtaaa gccaagcctt acaaggttaa tgcccaatgc
     1021 tttaagacgc ttgattctgt cgtcttccca tgagtaggca tactcacgga cgccgttagt
     1081 cggctttccc ttatccacta tgttaatgcc gttaaaaatt ctctcgcggc cgaattcgtc
     1141 aacaaaagcc ttacccttag tgagtatctt ttccataagg ctgcctccta atcaattact
     1201 ttacataatc aaaaatgggg gaatcctcgg gagcgtttac ggttttaagc gctgtgtcac
     1261 ggaatgcgtc cttgccaagc tcttttatgt cagtgaggaa atccaccgac tcaagagatg
     1321 tgcaggtaaa gaaggcgtat ttttcgatta cctttacgct tgcgggaatt tttacatatt
     1381 taagggaggt gcattcctca aaggctcccc tgcatatagt ttccacaccc tcagcgattt
     1441 gaaggtctgt aagcgcggtg ttctggaaaa acgcgctctg accgattgtt ttgcagcttg
     1501 aagggataac aaccttttca gcggcaaagc actttaagaa ggcgtatttc tcaattaatt
     1561 cggtgccttc gctgaggatt atctcctttg cggcggagca tttagcgaat gcccagtcgc
     1621 ctatggtctt aacagagggg agattgattt tctcaaggga tgagcaaaga tagaaagcct
     1681 ttgtgtcaat ggtctcgggc gttcctttaa actctacggt ctttaaactg gagcatccgt
     1741 taaaggcgct gacgcctatg taagaaacat ttgccccgat tttgacgctt tcaagattta
     1801 ctgcctccat aaaagagaag ttgccaataa cttttactgt gtcgggaatt ataaggcgct
     1861 ttacggtttt gttgccgtaa agggctttgt tttctatgcc cacaacggtc ttgccctcga
     1921 aagtctcggg gatgtcaagt gtatcgccgc ttcccgtata gccactcaga acaagactgc
     1981 cgtcgtttaa aaccttgaat acaaagctgt ctttagatag gtcgcttaca taggtttttc
     2041 cgaggatatt cgcggccttt acattggcgg ggtcgttggg gtcatctatt gtttcgctaa
     2101 tgtcgcagga gcaaagcgaa agcagaaggg ctaatagaca aagaaccgat aatactttat
     2161 atttcattat cttctcctcc ccttctttgc tgtttctttt tgctgtgcgg cctgctcctc
     2221 gatttctctg ttctgctgct tctcaagctc aagggcagcc ttggcttcct cgtacttctc
     2281 catcagctcg tcgaaatttg actggttgtg atattcacgc acgattctct gagcgtcgtg
     2341 gagcgcttta gtagcctttt tgtataagtc ctcggctttg tcaaggtctt taaaagcctt
     2401 tttcttttta agagcctctg caaagctgtt ttcggacatt gcctctacag ccgctctgtc
     2461 ttcgtcggtt atgggagtaa tgccgttcgg gaacttttta ataagcagtt tcttgcagct
     2521 aaggagctgt tttgcctcgg ttatggcgtc tgagcggatt gccttttcct ccgcggagtt
     2581 ggcgggcaaa gctcttgcct cttcaagaac tgtgtcgggc gtcttgtaaa gaccatctat
     2641 tcctgcctcg caaatcttcc gggctcttaa aagctttgcc tgcatgcggg gcgtattgaa
     2701 cttttcgatt tcgtctatga catataaagc ctcgactatc ttcttttgct gctctttgta
     2761 atcctttttg agcttctttt tctcagcttt ggctgccttc ttttcctctt tggtttttgc
     2821 ggcagctttg atttcctcaa tttccttttt aaggctctct ttattctctt tgcgctttaa
     2881 tttaagctct gagagccttt gttcttttgc cttcttttcc gtctcgttat gggtcttttg
     2941 cttctttatt atgtttatat agtcttttgc gatgatttcg tcttttttct ttatgttctc
     3001 ttttgcggcg ttaatgatgt caacaacctc aacaagagcg gagttgttaa taatgccgtt
     3061 gccgtagtct tcaagcagcg ccctgatttt aagaacgctt attatgcctt tgtgctttat
     3121 ttccgtaaag tcgtagaaga agtacgggat aacatttaaa gtagctccaa tgaccgcggc
     3181 gataatcaga acctctgaca tctcgtatct gaaggaggag acctcaagaa catcgtagtt
     3241 gtttacaagt ccgaggtgtt cgtaaatcat gggcagcacc atgcccgtaa acatgccaag
     3301 gaagctgccg ataagaccga cggtaccgaa cataccgtcg attctctcgc cgctgatgta
     3361 ttgctgatag tctctaatgt cagcggagat gcccacatcg taaacaaggg caaagtgttt
     3421 tgcaaaaccg ttgagatagt aaagcagaat cagtatatat atgttcttgt aggtgggcaa
     3481 aagggcgcct atgaaaatta tgtttgcgat attgcaccag ataagaagat ttctcttgcc
     3541 gataagtcta atgacgatgg gagcaagtag catcgaccaa aatgccgcgt tgccgattaa
     3601 ggttgtggca agggcatagg tgcccatgcg gtccgcgtag ccgtagacaa atgtccatgt
     3661 gagaactacc gcaacagcgt cctcaaggaa gcctatccag cctgctgtgg agataatcca
     3721 gaaatatttg tttttagaaa cagccttaag agcgcttata aagctgaaac tcctctcatg
     3781 ggagacagcc tcaactattc tttcctttac tccgaaaacg gtaaaggagg taagtattgc
     3841 gccgactatg gcgatggatg ggtgtataaa tctgtatgta gtgatattgt taagtccgcc
     3901 cgtaaatttt gagagaatcg gaactatgaa gttcgcaatg gagggagcca aggaatagag
     3961 aattgaagcg attgaaatta tgtcagttct ctcgttgctg tcgggggata taacattggc
     4021 gatattgttg tatgattctc gatagaatgg gaaaaagagc ataagcaggt tgtagcagat
     4081 gaaaaccgcc actaccttag agctgtatga catcgtttca tacggcagcc atacaaaaat
     4141 aattgaaata atgaccgtgg ggattgtggt aatggcaagc cagggtctga acttgcccat
     4201 cttaaacttt gctgtgtcta taagatagga tttaagaatc gtaatgccaa agcctatgat
     4261 tgtggaaatg acgctcatca gctgaagatg aacgggctga atgcctatgg tgtttcccac
     4321 aagcaaactg gaggaggtaa gggcgataag gccgccgaaa taaataatga actgaacgcc
     4381 tatgccgcct atgccgtagt taatgacctc tttaagattt acatacttgc cctccggggg
     4441 ctttttccag tagcgtctta tataatttat aacgctcttt gccttagtta aaatgccttc
     4501 attcattgca ttacacccat ctcggtttca agatttcagt ttttcagtca aaattataga
     4561 cattgggata aatcccgatg tctgtttctt cattaaatat aaacaattaa gggcattaaa
     4621 atatgctata attatagcaa tttattcaac acatttcaac aaaaagacgg ctattaatac
     4681 aaagttaaca aacgctcaaa aattaaggca tatattgatt tacactattg ataatcgtta
     4741 ataattagca ctgttttgcg attaaaggta aaaaagcacc cgtatttcag ggtgcctttt
     4801 tattatcttg cctgaatctc aaacagcttt ctcatggagt ggttgtcatc cacaatctca
     4861 tcatagaaga tgtgagtccc catattgtgc ctttcggact ttacggcgtc gtaggcaaag
     4921 tggattctgc cgtttaccat aagttttgcg gcgctccacc aagaattcat aatgtcgtcc
     4981 tctgtgaact taacatcgaa ttcatagcgc tccttgctgt gaggtttgag acttacggga
     5041 attgcgcagg ggccaacctc tccccttgcg gcggtgttaa agcctaagag agaccatgtt
     5101 tcaaagggtg tcgcaagggc gagctcgccg tttaagtgtg aatcggtatc gttggtgacg
     5161 gcgactttga ccttgttgtc ttctatcttg agccttactt taggattaaa gtagccgaat
     5221 tcaaagatgt cctcaaagac ctgaccgtcg tgggtgaact gaagtttaat aaagcctttg
     5281 gcgtcctttg cgggctttgt gacaaccacg gaatactcaa gaaggctgtc gggggcaatt
     5341 tcatagggga actcggtttt gtcagcctta aagccgtcgg ggagaatgag ctttaatact
     5401 cccgattcgc tcttgtcggg ctgattgttg gcaagggaga tgccgagtct gacattcagg
     5461 tcatcgagag cctcgacctt tctgcttata gcggcgacca gtctgtaata gcccatgggc
     5521 attgagccga ggtcgtgctc atagctccta atataagtgg gctcggcgat ttctcttgtt
     5581 ctgccaaggg gatatgaggc tgaaatggta ttctgcggct ttttgacggc gatattgtag
     5641 gtttcgattg agtgggtcgg aataaaggcg gtaaagccgt ttttgccgct cttaacctca
     5701 gtttcgttct cgtcaagcag gtcggtgttt ttgaccttgc cgatttcaaa gtcgaactta
     5761 aacattgttt cgcagtctgt accgttcgac tcaaagccgc ggactgtgat gcctctttca
     5821 aatacgctct taataccacc ctccatttta gcaaggggat agccgcccgc cttgaaggct
     5881 gtgactataa aggtgccgtc ggtcttaaag aaggatttgc tttcgggcag gaaggggttt
     5941 tcggcggttt caaattcggt tacggcgaac atgggctcgt taaactccat gcccttttta
     6001 aatagctctg cctcacggta gcttttttca tgggggtaga ttgcgtactc gaaaacatgg
     6061 gttttgtttt cggggataag ctcggtgctg cccgttacct tgccaatgtt gccgtagaac
     6121 tcggcggtgt ggaagagcat catgttgagc atattctcgc cctcaacact gcaggcgatt
     6181 gtgcccttgt tgaggattcc gagaccgaag tcatcgatag cctcaagctc ggaggcgatg
     6241 accgctcccc taatctcagc cgcatagccc atggagtatg ctttttcaag ctcgtcaaga
     6301 taggcgttca gggtatatac gctctttgcc tttataagaa gcacatcaat gggctttttg
     6361 aagtaattgt ctgagtcctt gatatagaga gccgcgacgc cttttttgtt gagaaggttt
     6421 tggaattttt cacgctgctt ttcagtcagt ttcgaaagca gctcatcttc atacagattc
     6481 cgaacgccct cgaccgagag cacaaagcgg gtatcggtgt aggaaatatc ctcgttaaag
     6541 tgaatgattt ttgtagctct gcgctcggtg cggtcatggt aaggcgttac gggaatggcc
     6601 tttttgctta aggtcttaag cagtttgttt gcagagagga ttaaatcctt ttcctcgggt
     6661 ctgataattg ccgtcatgcc gatattgagg ctgcccttga gtctgcctgc ggagtttgtg
     6721 aatttgagct gggtggtggg acccaagtca aaccaattga cgctgggggc aacctgacag
     6781 cgggaataca taaagaactg gtgggtttgg aaggacagct tctttgcgct ctttgctgtg
     6841 acatggggcg cgaatctatc ctcgtaaaca ggctttacgc cttttaatgc ggcggggaat
     6901 gtgactgtga acatatcgtc gttgtctatg tagtcctcga cgatggtttt gcagtctatc
     6961 ttctttgagt ttttgtaaag ggtgatttcc tgccttaccc ttgccacaac atcgagcctt
     7021 acggttacta tcagcttatc gtaaagcgag gtcttttcac tgcttacatc ggctatgaat
     7081 tcatcggaga acaacttgtg accagtagtg tagaactcgt gctgggtttc cattctgttg
     7141 tggatttcct ttaaaacagc gattctgttt gcgggaccgt cagtgctcgg gtctatgaat
     7201 tcacgcttgt tgactttatc gtaaatggat acgattccgc cgcccctttg cgggtcaacg
     7261 gtgaccttga agtacttgtt ttcaatgctt gtatccttgg atttgatgac ggttttcggc
     7321 ttatcggtct ttttaaggta gaaggtctta tagccgaatg cggggacctt tgcaataaca
     7381 accgctttaa gtttcttgcc gtcgtgagtt ttgcctatta tctcaaagct gtgctccttg
     7441 ccctttgaat caaagagggc atagccgctc tcagcccaca atggcagggt aaattcaata
     7501 gcctcgtccc tgtcccatgt gtgctggttg aatatgatta cggggaaggc ttcatcgctg
     7561 cgcttaattg cggaggcgat aaattccgat gcgttcttta cgatttctgc tgcgagctcc
     7621 gctgcttcac gatattcaat cataaggtca acgaaggaga cttcgttgtt tgtgcctgta
     7681 attgagtcgt ggtgctgggc gcaaaggagc tggcgccatg ccttatcgag agctttttcg
     7741 ggatactccg cgccgtaaag ggccgctatg gtgctgaatt tctccgcggt gacaagcagg
     7801 ttctctataa gtctgttagc ctgcttgtaa tcggttcttg taagcagaac agcggcgtgg
     7861 tgttgggtca tatcccttga ggtgtactct attttggaaa tgcccttttc cttaaccgtt
     7921 tctttttcgt cctcaataat gccgtccatc atgtttgaga acagcgagaa ctttgcgccg
     7981 ttgagggtct ttgacatcca tgaggtgtcg ccctccttgg ggtacaattc aaagccggcg
     8041 ttcactttgg ttgacctgtc gcagtgggta attcctaagc gcatagcgtt ttcaacgctt
     8101 atgcccttgc tgtggagaat gtcgctgccg tcgggggaaa catggtggaa cagcgcgtca
     8161 agacctataa tcatcttgcc ccatcttgca ctgttgcagc cgcccttgcg gcaaatctgg
     8221 gacatctggt tcgggtgtcc gaaaacatcg ccgggtgaat agacgctgac tattctgccc
     8281 aagacatctc tgtggtatag ctgaccgtaa atgagatttc tgacaatcgc ctcgcccgat
     8341 gaggtcagct cattgggctg gttgtagaag cagtctgcct ctaccctgcc cgttttgtat
     8401 gcctctttta gtatctttct gcactccggg taaagggaat atacggggtg cagatagtca
     8461 atctcggata tggtggcggt gaacaggggg ttccttgcaa tctccttaag tatctccata
     8521 gtaatgtaga tggcgccgat tgcgtaggtc ctctgctcct gatgatatgt ggtgtcaaag
     8581 tggaagtcgg taaagatatg ctccgtcccc acaaagccgt tgtagggcgt cagctcataa
     8641 ttattagagt aattgcccga gggatttgcg ccttctttca gctcaaggct gatttccgcc
     8701 gtaaagggct tatcctttac gggggcagaa gtatatataa ggcttgtgga gcctgcggtc
     8761 atgggctcaa tattatgaat ttcggtatag tcgccgctct tgagtgtcag ctctgcgcag
     8821 tttgtttcgg aaagggcgcc gacaaagagg ggcagaatct gtctgggctc ttccttagtg
     8881 ccgaaatatg cggtagaggt aacgggaggg gtaattacca cattgcccga ccgagccacg
     8941 acgggataga tttcacagtt gctcatgctt atgtcaatgc tgtcagctat gtagccgacc
     9001 ctgattttaa acatcaggag gttcctgccc tttttaaagc gcacaaggac tctttcgcct
     9061 attctgccaa gtcctttaac tctgccgtag ggtgtattgc ttaccagctc gccgttgaga
     9121 tacaaaaggc agccggagtt tatgtagcag attacggcgt cctgctcttt gtcggagttt
     9181 atgtaaacag ccgcatagta gacgcagttt ctctgctcgg tgcggaaaag cgcctcgtcg
     9241 cagctttcat aggggttgtc aaatctcaaa tcaccccatt ttctgatgcc ttttctccaa
     9301 gttaagaatt cggggccgta gtacttgttc tttacctgct tgccgagata cggcacttct
     9361 gacgcctcgc cgccggattc ggcaaggtag tcgtggtcaa ggaccttgtg tctttcgtaa
     9421 aaatactcgg tttcaaaggc tccgtcggtt tcaagaacaa agggtcccaa agtcataaag
     9481 tcatagagtt tatattcggg tgatggatta tcttttgtgg gaataaagtc agaaagattg
     9541 tattcgcatt tttccgtctg cgaaacggct gaataatcag cttttccggc catgattacc
     9601 ctcccaaacc aatattttta cttatagtac aatataccgc cggttaattc aatcatatat
     9661 tgaaaaacat acatta
//
LOCUS       DAONVQ010000077         9593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5474_length_9593_cov_4.710317, whole genome shotgun sequence.
ACCESSION   DAONVQ010000077 DAONVQ010000000
VERSION     DAONVQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5474_length_9593_cov_4.710317"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLD98_05925"
     CDS             complement(<1..552)
                     /locus_tag="PLD98_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQA05820.1"
                     /translation="MKKIYDIIDSVETLEKAFKDIREAQKVFATYTREQVDKIFLSAA
                     TAANKARIPLAKMAVEETGMGVVEDKVIKNHYASEYIYNAYKDAKTCGVIEEDKFYGI
                     KKIAEPLGIIAAVIPTTNPTSTAIFKALLALKTRNGIIISPHPRAKNCTIEAAKIILE
                     AAVKAGAPEGIIRWIDVPSLELTN"
     gene            921..1844
                     /locus_tag="PLD98_05930"
     CDS             921..1844
                     /locus_tag="PLD98_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQA05821.1"
                     /translation="MNIQHLKYAVEVEKAASITQAAENLYMSQPNLSKAIKELEAYLG
                     APIFRRTSKGMIPTAKGQTFLAYAKNILKQVEEMESLYRPSDPSRQVFSLSAPRTGYI
                     SMALVNFAEKLDRKKEIELNYKETNATKTINNVVRGEFSLGIIRCRTEHEKYFFGTLD
                     DKEISHEKLWEFDYCVLLNEKSPLALKQEIRPEDLEGMIELDHGDTTVPPASVQQKEI
                     RPAKDSRKIYIYERGSQFDLLSRFTDAYMLTAPEPEELIKKHGVVQKKLTGVKLRYCD
                     ILIHLAEYSPTELDKMFLNELKKASRILMNP"
     gene            complement(1875..3896)
                     /locus_tag="PLD98_05935"
     CDS             complement(1875..3896)
                     /locus_tag="PLD98_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQA05822.1"
                     /translation="MNRLQYEKSPYLLQHAYNPVNWYPWGEEAFEEAKRQDKPVFLSI
                     GYSTCHWCHVMEKESFEDDEVAQILNRDFIAIKVDREERPDIDAVYMAVCQALTGSGG
                     WPLTILMTPEQKPFWAGTYLPKRARYGIIGLIDLLQRIAHLWKENRELLIKDSEKIIA
                     YINRQKTARGISPERSLIHRAYKDFKASFDEEFGGFGSAPKFPTPHNLIFLLRYSQVE
                     VKKTALNMAEKTLLQMYRGGIFDHLGGGFSRYSTDDMWLVPHFEKMLYDNALLTLAYL
                     EAYRITQKALYLSVAERILGYVLRELRDEKGGFYCGQDADSDGVEGKFYVFTKEEIKS
                     VLGEGADAFCEWYGVSDEGNFEGKNILNLLYNPRYESSNPEIERKREKLYEYRAKRTS
                     LHKDDKILTSWNALMIAALSKASLITGKEEYLSAAVNAYEFIMVNLTDDGGKLKIRWR
                     ENESAFEGQLEDYAFFAFALLELYETTLNAEYLEKAAHFAEKMTELFMDEKNGGFYFY
                     SKEGEQLISRPKETFDGAMPSGNSVTADVLFRLWKLTGEEKWQEISSRQFSFIAEAAL
                     NYPTGHSFALIALSRALYPSYDLVCVSSEEKAPAELREFLKDGCYPNLSCVLKTNENK
                     ELLSKIAPFTDSYPIPEKGSAYYLCENGACAAPVFSIEEVKRMLGSGQI"
     gene            complement(3914..5374)
                     /locus_tag="PLD98_05940"
     CDS             complement(3914..5374)
                     /locus_tag="PLD98_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA05823.1"
                     /translation="MESIWTQTTEIQSYDKLTEDMTADAVVIGGGMAGILTAYKLTQN
                     NIDTIVLEASRIGSGQTKNTTAKITSQHDIIYSRLTEDFGEDLARQYAAANENAIREY
                     KRIIEKENIDCNFEEKPAYLYSQNQQDVKRLQDEADAARELGIDAEYTTETHLPFSVA
                     GAVKFNNQAQFHPLKFLKEIAKGLTIFEDTRVIKVEEDTVFTETAKVRAKHIIFATHF
                     PFINAPGFYFARMHQERSYVLALKGAAKLDGMYLGVDDDGISLRNFDDMLLFGGGNHR
                     TGENSAGGKYDFLRAKAKEFIPQSKEIACWSAQDCITLDGVPYIGQYSSSVLNWYVAT
                     GFAKWGMTSSMVSAMIISDMILGRENPNAEVFSPSRFTPSASTKTFIEDLGQSVKGLA
                     RQFFTFPDEKAERLRAGSGGIVEIAGEKVGVYKDEEHKLHIVSVKCPHLGCQLEWNPD
                     EKSWDCPCHGSRFDYRGNLIDNPAQTNLERIDTSQS"
     gene            5481..6185
                     /locus_tag="PLD98_05945"
     CDS             5481..6185
                     /locus_tag="PLD98_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05824.1"
                     /translation="METKKERVITAVSRIFTVGALWGIFEATVGYLLHLLPFNIGWLV
                     WYPAACFFMYLAYQKSERTADILLVALLASGIKLLNLFMPIRIDRVINPAVSIILEAA
                     AMFAVIKLRNKYLENKKKNIPILAFSVLLMNTLWRALYILYILLLVPEWIRNISVISS
                     TEALINFLIEQNFYTCLIIFAGAVLFNPLMKPIKRIEKRAESRLLKLPVRVLLSIKLT
                     AAIVLLIINISLQFAL"
     gene            complement(6215..7456)
                     /locus_tag="PLD98_05950"
     CDS             complement(6215..7456)
                     /locus_tag="PLD98_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05825.1"
                     /translation="MNPFEQKPMNLTDGIMDWCTVYPKPYCKNTVDPYTKVRIILMNG
                     IEVEAIIFKHQFSRNCNNNDIRRELALTRRIEQQQQKHINWLKPIDETPLETTIGYEH
                     VAVDLTAWLAQNEPDPYVKQALDFALLEDFDHLYRYANLLDLDAQIPAQQLVKSYVDI
                     TPGRPTIAEHRFPYDSIKYHVDFKKADLQTMLNTLIITAGEQQTMNFYMNIGNTYYND
                     LGRELYLEIGMIEEQHVSHYGSLLDPNCTWLENMLLHEYTECYLYYSFYEDETDPNVK
                     SIWEMHLQQEIAHLHKAAEMLKKYENKDWQQVIPGGDFPKLLEFHDTRDYVRMILDKQ
                     ILLTANREQYAPVSDLPADHEFFFYQNKVNHNVDAVPSHKVIVQHQQKFNIDYRTESN
                     PNPVQALTDRTMDNTTIARTK"
     gene            7577..>9593
                     /locus_tag="PLD98_05955"
     CDS             7577..>9593
                     /locus_tag="PLD98_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HQA05826.1"
                     /translation="MERAVGISIQRKEAWDKVTGRAKYTDDFPVTDVLSARLLTSTYA
                     HAKIKRIDTSKAMAMRGVKAVLTGGDFKELFGPLLLDRPALARDVVRYAGEPVAMVVA
                     QDDCCAEEAARLIEVEYEQLPVILTPSEALAEGAPLIHRQPNGYKKVATDIYPIAGTN
                     IASKYQIRKGDAAAALKSSDIVIHRKFCLPPSDHVAMEVRTAQAEISADGTVLITTSS
                     QAPYTVRKMLSEVFMIPAGKIQIKVPFVGGGFGGKAPVFLEILALLASMSVGGRPVRL
                     TIPREQDMASAPSRIGLEADIKIGATKDGVFTGAEMTYLLDSGAYTDITPYMTKATAV
                     DCTGPYRIDNLSCDALCVYTNHTYSTSYRSFAHESLTFCVERTIDALAKECGIDPLEI
                     RLKNAIVPGCSTPTGVYCTEDIIGNLPECLRKVRELSRWNGGRAEAIKKDAIKALGIA
                     CFWKTETPPTNAVSGALITFNPDGSVNLNTGVVEIGSGDQTHLAQMLAERLKISPKDV
                     HVVMDVDTRTAPEHWKTVASITEYMAGNAVIEAADDMIAQLKTIGSEALKCPPEEILI
                     ENGRVFSRKNPQNFIAFKDIALGYKSADGESIGKPVIGRGGFMLKGLTELDTETGQGK
                     TGPSWTVGAQVVEIEANLKTCTYRITNASTVMDVGKAVNPELMRSVIA"
BASE COUNT         2417 a         2370 c         2270 g         2536 t
ORIGIN      
        1 gtttgtaagc tcaagggacg gcacatcaat ccatctgata attccctctg gcgcgccggc
       61 ttttacggca gcctcaagta taatctttgc agcctctatg gtgcagttct ttgctctcgg
      121 gtgggggctg atgattatac cgtttcttgt cttgagagcc aaaagagcct taaaaatcgc
      181 cgttgaagtg gggtttgtgg tggggataac cgccgcgata attccgaggg gctcggctat
      241 tttctttatt ccgtagaact tatcctcttc aatgactccg catgttttag cgtccttgta
      301 tgcgttgtag atatattccg aagcgtaatg gttctttatt accttatcct caaccacgcc
      361 cattcccgtt tcctcaaccg ccattttggc aagagggatt ctcgccttgt ttgccgcggt
      421 tgctgcggag aggaagattt tgtctacctg ctcccgggta tatgtggcga aaaccttctg
      481 cgcctctctt atatccttaa aagctttttc cagtgtttca acgctgtcta ttatatcgta
      541 aatcttcttc atatccgttc tccttattcg aaaagggtag agtagttgaa cttaatttcc
      601 ctttattaat tagtggaatt acctgttgcc gctgatgaaa acttttcctg ccctctacag
      661 tggggtcccg tcattgtggt taagcaaagc ggcaaacgct gtattgtcag cttattcatt
      721 atcaacaccc ctttcactta cagtctatca ttggcagcat ttagcagcaa ttatataaat
      781 ggaatataag tattccttta tcgatatggg attatcgata ttgtcgtatc tattcaaatt
      841 ttcggttgaa ctattgtgca aaagtgctat aatgtaaaaa agaaattcat ttgtcaataa
      901 tccctttatg ggaggtaaat atgaacatac agcatttaaa gtacgcggtg gaagtcgaaa
      961 aggcggcgtc cataacacag gcggcggaga acctttatat gtcccagccc aacctcagca
     1021 aagccatcaa ggagcttgag gcatacctcg gcgcgccgat tttcaggcgc acctcaaagg
     1081 gcatgatacc caccgccaag ggtcagacct ttcttgcata cgcaaaaaac atcttaaagc
     1141 aggttgagga aatggagtcc ctctacagac cctccgaccc ttcaaggcag gttttcagcc
     1201 tatccgcccc gagaacgggt tatataagca tggcgcttgt gaactttgcg gaaaaactgg
     1261 acaggaagaa agaaatagag cttaattaca aggaaaccaa tgccaccaag accataaaca
     1321 atgtggtaag aggggagttt agtctcggca taatccgctg cagaacggag cacgaaaagt
     1381 actttttcgg cacccttgac gataaggaga tttcccacga aaagctttgg gagtttgact
     1441 actgcgtgtt gctgaacgaa aaaagccccc ttgccctaaa acaggaaatc cgccccgagg
     1501 accttgaggg catgatagag cttgaccatg gcgacaccac agtgcctccc gcctccgttc
     1561 agcaaaagga gataagaccc gccaaagaca gcagaaaaat atatatttac gaaaggggca
     1621 gccagtttga tttgctctcg aggtttaccg acgcgtatat gctgacagcc cccgagccgg
     1681 aggagcttat aaaaaagcac ggggtagtcc agaaaaagct gactggagta aagctcagat
     1741 actgcgacat actcattcac cttgctgaat acagccccac agagctcgac aagatgttct
     1801 taaacgagct taaaaaggcg tcaagaatac tgatgaatcc ataagaaacg ggactttggc
     1861 aaagccaaag tcctttaaat ttgcccgctt ccaagcatcc ttttaacttc ttcaatgctg
     1921 aaaacgggag cggcgcaagc cccgttttcg caaagataat acgcgctgcc cttttcgggg
     1981 atagggtagc tgtccgtaaa aggagcgatt tttgaaagca gctctttgtt ttcgttggtt
     2041 ttcagaacac agcttaaatt gggatagcag ccgtctttca aaaattctct cagctccgcg
     2101 ggtgcctttt cttcgcttga tacgcaaaca aggtcatagg aagggtacag cgcccttgaa
     2161 agagctataa gcgcaaagct gtgacccgtg gggtagttca gcgccgcctc agcgataaat
     2221 gaaaactgcc ttgagcttat ttcctgccac ttttcctcgc ccgtcagctt ccatagtcta
     2281 aacagcacat ccgcggtgac cgagttgcct gaaggcatag cgccgtcaaa ggtctctttg
     2341 gggcgggaga ttagctgttc gccttctttt gaataaaagt agaatccgcc gtttttttca
     2401 tccatgaaaa gctctgtcat tttctccgca aagtgagccg ccttttccaa gtattcggca
     2461 tttaaggtcg tttcataaag ctccaaaagg gcgaaagcga aaaaggcata gtcttcaagc
     2521 tgaccctcaa atgcgctttc attctcccgc catctgattt ttagtttccc gccgtcgtcc
     2581 gtaagattca ccatgataaa ctcatacgcg tttactgcag cgctcagata ttcttctttt
     2641 cccgttatca gcgacgcctt tgaaagcgcg gcaatcataa gggcgttcca agaggtcaga
     2701 atcttgtcat ccttatgaag acttgtcctt tttgccctgt attcatacag cttttcccgc
     2761 ttcctttcaa tctcggggtt tgatgattcg taccgaggat tataaaggag attcagaata
     2821 ttcttcccct caaagttgcc ctcatcggac accccgtacc attcgcagaa agcgtctgcg
     2881 ccctcgccca aaacgctctt tatttcctct ttggtaaaga cataaaactt gccctcaacg
     2941 ccgtcgctgt cagcgtcctg accgcagtaa aagcctccct tttcgtccct cagctctctt
     3001 aaaacatagc ccaaaatgcg ctcagcgacg ctcagataca aagccttttg ggtaatccga
     3061 tatgcctcaa ggtatgcgag tgtcagcagg gcattgtcat agagcatttt ttcaaaatgg
     3121 ggcaccaacc acatatcgtc ggtggagtag cgggaaaagc ctccgcccag gtggtcgaat
     3181 atgccacccc tatacatctg caaaagagtt ttttccgcca tatttagggc ggtttttttg
     3241 acctcaacct gagaatacct taataaaaat atcagattgt ggggcgtggg gaatttggga
     3301 gcggagccaa agccgccaaa ctcttcatca aaggatgctt tgaaatcctt gtacgccctg
     3361 tgtatgagcg acctttcggg gctgatgccc cttgcggttt tctgcctatt tatataagct
     3421 attatctttt cgctgtcttt gattaatagc tcccgatttt ccttccaaag atgggcaatc
     3481 ctttgcagca ggtcaattag ccctataatg ccgtatctcg ctcttttcgg cagatatgtg
     3541 cccgcccaaa atggcttttg ctcgggcgtc atcaatatgg tcaaaggcca tccgcccgac
     3601 cccgttagag cctgacaaac agccatataa accgcgtcaa tgtcgggcct ttcttctcgg
     3661 tcaactttga ttgcgataaa gtccctattt aaaatctgag ctacctcatc gtcctcaaag
     3721 gactctttct ccatcacatg gcaccagtgg caggtgctat agccaatgct taaaaatacg
     3781 ggcttgtcct gccttttagc ctcctcaaag gcttcttctc cccacgggta ccagttgact
     3841 gggttgtaag catgctgcaa aagatacggc gacttttcgt actgtaagcg gttcactcct
     3901 gctcctccta tgctcagctt tgagatgtgt ctattctttc aagatttgtc tgagcggggt
     3961 tgtcaatgag attgccccta tagtcaaagc gggagccgtg gcacgggcag tcccagcttt
     4021 tttcgtcggg gttccattca agctgacagc caagatgcgg gcacttaacc gacacaatgt
     4081 gcagtttatg ctcctcgtcc ttgtaaacgc ccactttttc gcccgctatt tccacaatgc
     4141 cgccgctgcc tgctctaagg cgctccgcct tttcgtcggg gaatgtgaaa aactgccttg
     4201 ccagcccctt gaccgactgt cccaaatcct cgatgaatgt ttttgtagaa gcagagggag
     4261 taaatcggct cggtgagaaa acctcggcat tgggattttc tctgcccaaa atcatatcgg
     4321 aaataatcat ggcggatacc atgcttgaag tcatgcccca ctttgcgaag cccgtcgcta
     4381 cataccagtt gaggactgaa gatgaatact gcccgatata aggcacgccg tcaagggtaa
     4441 tgcaatcctg agcggaccag catgctattt ccttgctttg cggaataaac tcctttgcct
     4501 tagcgcggag aaaatcatac tttccgcccg cgctgttttc gcccgtgcgg tgattcccgc
     4561 cgccaaacag gagcatatcg tcaaagtttc taagggagat tccgtcatcg tcaacgccca
     4621 aatacattcc atcaagtttt gcggctccct ttaacgccag aacatagctt ctttcctgat
     4681 gcattctggc aaaataaaat cccggagcgt ttataaaggg gaagtgggtg gcaaagatta
     4741 tgtgctttgc cctgaccttc gcggtttcgg tgaaaaccgt atcctcctca acctttataa
     4801 cccttgtgtc ctcaaagatg gtaagcccct ttgcgatttc ctttaagaac tttaaggggt
     4861 ggaactgcgc ctgattatta aacttaaccg ctcccgcaac cgagaagggc aaatgggttt
     4921 cggtcgtgta ttcggcgtca atacccagtt cccttgccgc atccgcctca tcctgaagtc
     4981 ttttaacatc ctgctgattc tgggagtata aatatgcggg cttttcctcg aaattgcagt
     5041 ctatattctc cttttcgatt attcgcttgt actcccttat ggcgttttcg ttcgccgcgg
     5101 cgtactgcct tgccaaatcc tcgccgaaat cctcggtaag ccttgagtaa ataatgtcat
     5161 gctgagaggt aattttcgcg gtggtgtttt tggtttgacc gcttcctatt ctgcttgcct
     5221 ccagcacgat tgtatcaata ttgttttgag tgagcttata tgccgtcagt atccccgcca
     5281 ttccgccgcc aataaccaca gcgtcggcgg tcatatcctc tgtgagctta tcatagcttt
     5341 gtatctccgt cgtctgagtc catatagatt ccataatttc acctcgcaga tagagtatgt
     5401 aaaaagcccg aaaataaacg aagaagctga attttttaat ttctcgggac atatctttaa
     5461 aggagattgg aggggataat gtggaaacaa aaaaagagag agtgataacg gctgtgagca
     5521 gaatcttcac cgtcggcgct ctgtggggaa tttttgaggc aaccgtggga tatttacttc
     5581 accttttacc ctttaacata ggctggcttg tttggtatcc cgccgcctgc ttttttatgt
     5641 accttgctta tcaaaagtca gagagaaccg ccgatattct gcttgtcgcc cttcttgcct
     5701 cgggcataaa gcttcttaat ctgtttatgc ccataagaat cgacagggtg ataaatcctg
     5761 cggtatcgat aattcttgaa gccgccgcca tgttcgcggt tataaagctg cgaaacaaat
     5821 accttgaaaa taagaaaaag aatattccca tccttgcttt ttcggttctc ttaatgaaca
     5881 ccctctggcg cgccctttac atactctata ttcttctttt ggtgccagag tggataagaa
     5941 atatctcagt catcagcagc acagaggctt tgattaattt tctgatagaa caaaacttct
     6001 acacatgcct cataattttc gcgggagcgg ttttgtttaa tccgctgatg aaacccataa
     6061 agagaataga gaaaagagca gagagccgct tgctaaagct ccccgtcaga gttttgctca
     6121 gtataaaact tacagccgct atcgtgctat taatcataaa cataagcctg cagtttgccc
     6181 tgtaaataaa aaaaccgccc ttttggggcg gtttttattt ggttcgggca atcgtggtgt
     6241 tgtccattgt cctgtcggtc agcgcctgaa cggggttcgg attggactcc gtgcgatagt
     6301 ctatattgaa cttctgctga tgctgaacaa taaccttgtg gctgggtacc gcatccacat
     6361 tgtggttgac cttgttctga tagaagaaga actcatggtc agcgggcaaa tcggaaacgg
     6421 gagcatactg ctctctgttt gcggtgagca gaatctgctt gtccaaaatc atgcggacat
     6481 aatcccttgt atcgtggaat tcaagcagct tggggaagtc gccgccgggg ataacctgct
     6541 gccaatcctt gttttcatac ttcttgagca tttccgctgc cttgtgcaga tgagcgattt
     6601 cctgctgcaa atgcatttcc caaattgact ttacattcgg gtcggtctcg tcttcataga
     6661 aagaatagta gaggtagcac tcagtgtact cgtgcagaag catattctca agccatgtgc
     6721 agttggggtc aagcagcgag ccgtagtgtg acacatgctg ctcctcaatc atgccgattt
     6781 caagatatag ctctctgccc aggtcgttgt aataggtgtt gcctatattc atatagaagt
     6841 tcatggtctg ctgctcgcca gcggtgataa tcagcgtgtt gagcatggtc tgaaggtcgg
     6901 cctttttaaa gtctacatgg tacttaatag agtcataggg gaagcggtgc tcagcgattg
     6961 tgggacggcc gggggtaatg tccacatagc ttttaaccag ctgctgagcg ggaatctgcg
     7021 cgtcaaggtc aagaaggttc gcgtatctgt aaaggtggtc aaaatcctcc agcagagcaa
     7081 aatccagcgc ctgctttaca taggggtcag gctcgttctg agcaagccat gcggtgaggt
     7141 caactgcgac atgctcatag ccgatggtgg tctcaagggg agtttcgtca atgggtttca
     7201 gccagttaat gtgcttttgc tgctgctgtt caatgcgcct tgtaagcgca agctctctgc
     7261 ggatgtcgtt gttgttgcag ttgcggctga actgatgctt aaagataatt gcctcaacct
     7321 caatgccgtt cataaggatt atgcgcacct tggtgtatgg gtcaaccgtg ttcttgcagt
     7381 acggcttggg atatacggtg caccaatcca ttatcccgtc ggtaagattc atgggttttt
     7441 gctcaaaggg attcattcca tcactcctta tgctttgtat actctaattt ttctcagaat
     7501 ttcattaaat atgcagtttc ggtataacaa aaggacatga gggaaaatta attaaagtaa
     7561 cagaaagggt gagggaatgg aaagggcagt cggcatcagc attcaaagaa aagaggcatg
     7621 ggacaaggta acgggcaggg caaagtatac cgatgatttt cccgttaccg atgttttgtc
     7681 cgcaaggctt ttgacaagca cctatgccca cgccaaaatc aagagaatag atacctcaaa
     7741 ggcaatggca atgagaggcg taaaagcggt tttgacgggc ggggacttta aggagctttt
     7801 cggaccgctt cttcttgacc gtcccgcgct cgccagagat gtagtgcgct acgccggaga
     7861 gcctgtggct atggtcgtgg ctcaggacga ctgctgcgcg gaagaggcgg caaggctaat
     7921 tgaagtggaa tacgagcagc tccccgtaat tctcacccca tctgaggcgc tcgcagaggg
     7981 cgcgccgctt atccaccgtc agcccaacgg ctataaaaag gttgcgacgg acatataccc
     8041 gattgcgggc accaatatag caagcaagta tcaaatcaga aagggcgacg ctgccgccgc
     8101 ccttaaaagc tctgatattg tgattcaccg caagttctgc cttccgccct ccgaccatgt
     8161 ggcaatggag gtaagaacag cccaggcgga aatctccgct gacggcacag tgctgattac
     8221 cacctcatct caagcgccct acactgtcag aaaaatgctc tccgaggtct ttatgattcc
     8281 agcgggaaaa attcagatca aggtcccctt tgtgggcgga ggctttgggg gcaaagcgcc
     8341 cgtattcctt gaaatcctcg ccctccttgc ctcaatgagc gtgggcggca gacccgtccg
     8401 tctgaccatt ccccgagagc aggacatggc aagcgcaccc agccgcatag gtcttgaggc
     8461 tgatataaaa atcggcgcga ctaaagacgg cgtctttacg ggagcggaaa tgacctacct
     8521 tcttgacagc ggagcatata cggacatcac cccctatatg accaaggcaa ccgcagtcga
     8581 ctgcacgggt ccctaccgaa ttgacaatct ctcatgcgac gccctttgcg tctacacaaa
     8641 ccacacctac tccacatcct acaggagctt tgcccatgag tcccttactt tctgcgttga
     8701 gcgcactata gacgctttgg ctaaggaatg cggtatcgac ccgcttgaaa taagacttaa
     8761 aaacgcgatt gttcccggct gcagcactcc tacgggagtc tactgtaccg aggatattat
     8821 aggcaatctg cccgaatgtt taaggaaggt gcgcgagctt tcccggtgga acggcggcag
     8881 ggctgaggct ataaaaaaag atgctataaa ggctttaggc atcgcctgtt tttggaaaac
     8941 agaaacgccc ccgaccaatg ctgtgtcggg agcgcttata acctttaacc ctgacggcag
     9001 cgtaaactta aatacgggcg tggtggaaat aggctcaggc gaccagaccc accttgccca
     9061 aatgcttgcg gagagactaa aaatcagccc aaaggatgtc catgtggtaa tggatgtgga
     9121 cacccgcacc gcacccgaac attggaaaac ggtggcgagc attaccgaat atatggcggg
     9181 caacgccgta atagaggctg ccgacgacat gatagcgcag ttaaaaacaa taggctccga
     9241 ggctctgaaa tgtccgccgg aggaaatcct cattgaaaac ggcagagtgt tctcacgaaa
     9301 gaatccccag aatttcatcg cttttaaaga tattgccttg ggatacaaat ctgcggacgg
     9361 ggagtcaatc ggcaaacccg taatcggcag gggaggcttt atgcttaaag ggcttacgga
     9421 gctggacacc gaaacgggtc agggcaaaac ggggccttca tggaccgtcg gagcgcaggt
     9481 tgtggaaatt gaggcaaacc ttaaaacctg cacctatagg ataacgaatg cctcaaccgt
     9541 tatggatgtt ggcaaggcgg tcaaccccga gcttatgcgc tcggtcatag cgg
//
LOCUS       DAONVQ010000078         9582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5479_length_9582_cov_4.360764, whole genome shotgun sequence.
ACCESSION   DAONVQ010000078 DAONVQ010000000
VERSION     DAONVQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9582
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5479_length_9582_cov_4.360764"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="PLD98_05960"
     CDS             <1..1015
                     /locus_tag="PLD98_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQA05827.1"
                     /translation="VSRILPEEDMPFLPDGTPLDIVLNPLGVPSRMNIGQVLEVHLGY
                     AARALGWHVMTPVFDGANENDIVDALKEAGLSPDGKIELYDGRTGEKFDNPVTIGTMY
                     FLKLLHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQE
                     ILTVKSDDVVGRVKTYEAIVKGENVPKPGIPESFKVLVKELQSLCLDVSVLDEENNVI
                     DLKQDYDDEEGSITSGFDVGSPIKPDDRDALDFMVSGYDNTSIIDVDRFDDDDEDSDE
                     DVVDSDDEFMEPDDITDDSLEDEDYHDVDADLIGDEDEVLPDDFDLSLDDIDSDLDDS
                     DLL"
     gene            1105..4731
                     /gene="rpoC"
                     /locus_tag="PLD98_05965"
     CDS             1105..4731
                     /gene="rpoC"
                     /locus_tag="PLD98_05965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQA05828.1"
                     /translation="MLNESNYNELNFESIKIGLASPDVIRGWSYGEVKKPETINYRTL
                     KPERDGLFCERIFGPSKDWECHCGKYKRIRYKGKVCERCGVKITKSKVRRERMGHIEL
                     AAPVSHIWYFKGIPSRMGLILDISPRELEKVLYFAKYIVIDPGDTPLQKYQIIDDKEY
                     EDMVEKYDEGSFRAGMGAEAIKELLCEIDVYKLSEELREELETATGQKRARLLKRLEV
                     VEAFKQSGNRPEWMILDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKK
                     LLELGAPDIIVRNEKRMLQEAVDALIDNGRHGKPVTGPNNRPLKSLSDMLKGKQGRFR
                     QNLLGKRVDYSGRSVIVVGPELKIYQCGLPKEMALELFKPFVMKRLVELDISKNIKSA
                     RKMVERGGAEVWDALENIIKDHPVLLNRAPTLHRLGIQAFEPVLVEGRAIKLHPLVCT
                     AFNADFDGDQMPVHVPLSAEAQAEARFLMLASNNLLKPSDGKPVTVPSQDMVLGSYYL
                     TLIKEGVKGEGKVFKDVEEAIMAYEEGELDLHARVKIRVTKEIDGEIRTGFINTSLGR
                     VLFNEPIPQDLGFVDRSDPEKMFDLEIDFVVDKKMLSKIVDRCIKVHGMSESAAVLDK
                     IKALGYKFSTKSGITVAVSDAVIPAKKKDYIAEAEREVERINQSYLMGECSELDRYRL
                     TIKVWETCTKKVTAELTTSLDQYNPLFMMVNSGARGSTSQLSQLAGMRGLIANTAGRT
                     IEIPIRSNYREGLNVLEYFISSRGARKGLADTALRTADSGYLTRRLVDVAQDVIIREY
                     DCGCKEGLTVRAITGEEGEVIESLEERLVGRFLLEPLIHPETGELLMGNDRPMTDDDA
                     KRIVEAGIEEVTIRSLLTCNSEHGVCIKCYGTNLATGETVTVGEAVGIIAAQSIGEPG
                     TQLTMRTFHTGGVVSTDDITQGLPRVEELFEARKPKNLAIISRIDGVVSFEEVKRAEY
                     VVVTNMEEQEKYLIPYGYRKCVEEGQHIKKGDVITEGSINPHDLLEVLGVQAVHNYLI
                     QEVQSVYRLQGVEINDKHIEVIVRQMMRKIKVVDPGSTDLLPGATVERREFYEANAAV
                     ERRAAETGEDLKPAIGQPVLLGITKASLATESFLSAASFQETTRVLTDAAIKGKTDPL
                     LGLKENVIIGKLLPSGTGMKCYGDVKVVPQHKKTADEESSASSEGSSETIRLTSLADL
                     ADLR"
     gene            5053..5472
                     /gene="rpsL"
                     /locus_tag="PLD98_05970"
     CDS             5053..5472
                     /gene="rpsL"
                     /locus_tag="PLD98_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HQA05829.1"
                     /translation="MPTFNQLVRHGRQSVEKKSKSPALGKSLNSQKNILLDQNSPQKR
                     GVCTVVKTDTPKKPNSALRKIARVRLTIGIEVTAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIIRGTYDTQGVNGRMQGRSKYGTKKAKAKK"
     gene            5637..6107
                     /gene="rpsG"
                     /locus_tag="PLD98_05975"
     CDS             5637..6107
                     /gene="rpsG"
                     /locus_tag="PLD98_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HQA05830.1"
                     /translation="MPRRGKVAKRDVLPDPLYNSKLVTKLINNVMYDGKKGVAQKIVY
                     GAFDIIREKTGKEPLEVFEAAMENVMPLLEVKARRVGGATYQVPMDVRPERRQTLGLR
                     WIVTYARQRSERTMKERLAHEIMDAVNSTGGAFKKREDTHKMAEANKAFAHYRW"
     gene            6141..8219
                     /gene="fusA"
                     /locus_tag="PLD98_05980"
     CDS             6141..8219
                     /gene="fusA"
                     /locus_tag="PLD98_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQA05831.1"
                     /translation="MPRQVPLDLTRNIGIMAHIDAGKTTTTERILFYTGVNHKMGETH
                     DGSATMDWMAQEQERGITITSAATTCFWKGHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VTVLCAKGGVEPQSETVWRQADRYKVPRLAYVNKMDIMGADFYKVVDMMKERLKCNAV
                     PIQLPIGSESSFRGVIDLVEMNATVYYDDLGLNIKVEEIPEDYKELADKYRIELLEHV
                     AEQDDELLEKYLSGEELTIEEIKACIRKATINNDMVPVVCGTSYKNKGVQKLLDAIVD
                     YLPAPTDVESIRGINPDTQEEEVRHSSDNEPFSALAFKIATDPYVGRLCFFRVYSGSV
                     TSGSTVYNSTKGKNERMGRILQMHSNHRQDIEVCYAGDIAAVVGVKFTTTGDTLCDER
                     HPIILESMEFPEPVIRVAIEPKTKAGQEKMGIALQKLSEEDPTFKYYTDPETGQTIIA
                     GMGELHLEIIVDRMLREFKVEANVGRPQVAYRETITKEAISDTKYARQTGGHGQYGHV
                     KIKIEPNPGKGFEFVNAIVGGVIPKEYIPAVEEGIKGAMQTGVLAGYNVVDVKVTLFD
                     GSSHEVDSSEMAFKIAASMAFKDAMKKAGPVLMEPIMKITVIVPDEYIGDVIGDINAR
                     RGEMRKMETRVGATHVHAFVPLSSMFGYATDLRSKTQGRGQYVLEPSHYTEVPKSIAE
                     SIIGERFKNL"
     gene            8323..9525
                     /gene="tuf"
                     /locus_tag="PLD98_05985"
     CDS             8323..9525
                     /gene="tuf"
                     /locus_tag="PLD98_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HQA05832.1"
                     /translation="MAKAKFDRTKPHVNIGTIGHVDHGKTTLTAAITKALALKGTSQF
                     VDYANIDKAPEEKERGITINTSHVEYQTETRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVIAATDGPMAQTKEHLLLARQVGVPYIVVFMNKVDQVDDPELLDLVEMEIRETLDA
                     YEFPGDDTPIIRGSALQALEYNGNDPNAPELKPIYELMDAVDSYIPTPDRKSDMPFLM
                     PVEDVFTITGRGTVATGRVERGQLKIGEEVEIVGLADESRKVVVTGIEMFRKILDYAE
                     AGDNIGALLRGVQRNEIERGQVLAKPGSIKPHTKFKGQVYVLTKDEGGRHKPFFSNYR
                     PQFYFRTTDVTGVIQLPEGVEMCMPGDNVVMSVELISPIAIEEGLRFAIREGGRTVGS
                     GVVIEVGD"
BASE COUNT         2622 a         2363 c         2396 g         2201 t
ORIGIN      
        1 tgtttcaaga atcctccccg aagaggatat gcccttcctg cccgacggca caccgcttga
       61 tatagttctt aaccccctcg gcgttccgtc ccgaatgaac atcggtcagg ttcttgaggt
      121 tcacttgggc tacgccgcaa gggctctcgg ctggcatgtc atgacccctg tattcgacgg
      181 cgcgaacgaa aatgacatcg tcgatgccct taaagaggcg ggtctcagcc cagacggtaa
      241 gattgaactg tatgacggca gaacgggtga aaagtttgac aacccggtca ccataggcac
      301 aatgtacttc cttaaactcc tccacctggt tgacgacaag attcacgcca ggtccacagg
      361 tccctactca ctggttaccc agcagcccct cggcggtaaa gcccagttcg gcggccagcg
      421 tttcggtgag atggaagttt gggctcttga ggcttacggc gccgcttata cacttcagga
      481 aatcctcaca gttaagtccg acgatgttgt cggccgcgta aagacctatg aggctatcgt
      541 aaagggcgaa aatgttccga agcccggtat ccccgagtca ttcaaggttc ttgtcaagga
      601 gcttcagtct ctctgccttg atgtcagcgt tcttgacgaa gagaacaatg taatagacct
      661 gaagcaggac tatgacgatg aagaaggctc catcacctct ggctttgatg ttggctcacc
      721 cattaagcct gacgacagag acgctctgga cttcatggtc agcggttatg acaatacctc
      781 aattattgat gtcgacaggt tcgacgatga cgatgaagat tcggacgaag atgtggtcga
      841 ctctgacgac gaattcatgg agcctgatga tatcacggat gacagccttg aggacgagga
      901 ttatcatgat gtggacgccg accttatagg cgacgaagac gaagtcctcc cggatgattt
      961 cgacctttca ctcgatgata tcgattccga tcttgatgac agcgatttgc tgtaattggt
     1021 ttcgggggct gatattgccc cctctgagac ctttacggct cggcaaaaga accgtaacac
     1081 tttaaattaa aaaggagcgg tcaaatgctt aacgagagca attacaatga actcaatttt
     1141 gaatctataa aaatcggtct cgcatctccc gatgtcatca gaggctggtc atacggcgaa
     1201 gttaaaaagc cggaaactat aaattacaga accttaaagc ctgaaaggga cggacttttc
     1261 tgcgaaagaa ttttcggccc cagcaaggac tgggagtgcc attgcggaaa gtataagaga
     1321 atccgctata aaggcaaggt ctgcgaacgc tgcggcgtta agattacaaa gagcaaggtc
     1381 cgccgcgaga gaatgggaca catcgagctg gctgctcccg tatcccacat ctggtacttc
     1441 aagggtatcc cgtcaagaat gggtcttatc cttgacattt ctcccagaga gcttgaaaag
     1501 gtactgtatt tcgcaaaata tatagttatc gatccgggtg acacacccct tcagaaatat
     1561 cagattatcg acgataaaga atatgaagat atggtagaga agtacgacga gggctctttc
     1621 cgcgcaggaa tgggcgccga ggctataaaa gagcttctct gcgaaatcga tgtttacaaa
     1681 ctgtccgaag agctcagaga agagcttgaa accgcaaccg gacagaaaag agcgcgcctc
     1741 ttaaagcgtc ttgaggttgt tgaggcattt aagcagtcgg gcaaccgtcc cgagtggatg
     1801 attctcgatg tcatccccgt tattccgccc gaccttcgcc ccatggttca gcttgaaggc
     1861 ggacgcttcg cgacctccga ccttaacgac ctttacaggc gagtaatcaa cagaaacaac
     1921 cgtcttaaaa agctccttga gcttggagct cccgacatta tagtcaggaa cgaaaagaga
     1981 atgctgcagg aggccgttga cgcccttatc gacaacggca gacacggcaa gcccgtaaca
     2041 ggccccaaca acagaccttt aaaatccctt tcggatatgc ttaaaggtaa gcagggccgc
     2101 ttcaggcaga acctcctcgg taagcgcgtc gactactcag gccgttccgt tatcgttgta
     2161 ggtcccgagc ttaagattta tcagtgcggt ctgcccaaag agatggctct tgagctcttt
     2221 aagccctttg ttatgaagag gctcgttgag cttgatatat caaagaatat caagagcgcg
     2281 cgcaagatgg ttgaaagagg cggcgcggag gtttgggacg cgcttgagaa catcatcaag
     2341 gaccatcccg ttctcctcaa ccgcgcacct acgctccaca gactcggtat ccaggcattt
     2401 gagcccgtac tcgtagaggg cagggcaatc aagctccacc cgctggtatg tacggcattc
     2461 aacgccgact tcgacggcga ccagatgccc gttcatgttc ctctttccgc agaggctcag
     2521 gcagaggcac gcttcctcat gcttgcatcc aacaacctgc tcaagccctc cgacggtaag
     2581 cccgttaccg ttccttctca ggacatggtt ctcggctcat actaccttac gcttattaaa
     2641 gaaggcgtaa agggcgaggg caaggtattc aaggatgttg aagaggcaat aatggcctat
     2701 gaagagggcg agcttgacct tcatgcccga gttaagataa gagttacaaa agaaatcgac
     2761 ggagaaatcc gcacaggctt tataaacaca tctctcggca gagttctgtt caatgagccc
     2821 attccgcagg acctcggctt tgttgacaga agcgaccccg aaaagatgtt cgaccttgaa
     2881 atcgactttg ttgttgacaa gaagatgctc agcaagatag tcgacaggtg catcaaggtt
     2941 cacggcatgt ccgaatccgc cgcagttctt gacaagatta aggctttagg ctacaaattc
     3001 tcaaccaaga gcggaattac cgttgctgtc agcgacgcgg ttattcccgc aaagaagaag
     3061 gattatatag cagaggctga gcgcgaggtt gaaagaatca accagagcta cctcatgggt
     3121 gaatgcagcg agcttgatcg ctacaggctt acaattaagg tttgggagac ctgcacaaag
     3181 aaggttaccg cagagcttac caccagcctt gaccagtaca acccgctgtt catgatggtt
     3241 aactccggcg cccgcggctc cacaagccag ctcagccagc ttgcgggtat gcgcggtctt
     3301 atcgcaaaca ccgcaggccg tacaattgaa atccccatca ggtccaacta ccgtgaagga
     3361 cttaatgttc tcgaatactt catttcctcc cgcggcgccc gtaagggtct cgcagacaca
     3421 gctctcagaa cggctgactc aggttacctt accagacgac ttgtcgatgt cgctcaggat
     3481 gttattatcc gcgagtacga ctgcggctgc aaggaaggcc ttaccgtcag agcaataaca
     3541 ggcgaggaag gcgaagtaat tgagagcctt gaagagcgtc ttgtcggccg cttcctcctt
     3601 gagccgctga ttcaccccga aacgggcgag ctgctcatgg gcaacgacag accgatgaca
     3661 gacgacgacg ctaagagaat cgtcgaagca ggcatcgaag aagttacaat cagatcactt
     3721 cttacctgca actccgaaca tggtgtctgc ataaaatgct acggtaccaa ccttgcaacg
     3781 ggcgaaactg taacagtcgg cgaggcagtc ggcattatcg ccgcacagtc aatcggcgag
     3841 cccggtacac agctcacaat gagaacattc cacaccggcg gcgttgtttc aacagacgac
     3901 attacacagg gtctgccgcg tgttgaagag ctgttcgagg caagaaagcc gaagaacctt
     3961 gctatcattt caagaatcga cggcgttgta tcattcgaag aggtaaagag ggcagagtat
     4021 gtggtcgtaa ccaatatgga agagcaggag aaatacctca ttccttacgg ctacagaaag
     4081 tgtgttgaag aaggtcagca cattaagaag ggcgatgtaa tcacagaagg ctccatcaat
     4141 cctcacgacc tgcttgaagt tctcggcgtt caggctgttc acaactacct catccaagag
     4201 gtacagagcg tttacagact ccagggcgtt gagattaacg acaagcacat cgaggttatc
     4261 gtccgtcaga tgatgagaaa gattaaggtt gttgaccctg gctcaacaga cctcctgccc
     4321 ggcgccacag tcgagcgcag agagttctac gaggcaaacg cagccgttga gagaagagcc
     4381 gccgaaacag gcgaggacct taagcccgca atcggtcagc cggttctcct tggtatcaca
     4441 aaggcatcgc ttgctaccga atccttcctc tccgcagcat ccttccagga gaccacaagg
     4501 gttcttaccg atgccgctat caagggcaag acagacccgc tgctcggtct taaggagaat
     4561 gtcatcatcg gtaagctcct gccttcgggt accggcatga aatgctacgg cgatgtaaag
     4621 gtcgttccgc agcataagaa gactgccgat gaggaaagct ccgcttcctc cgaagggtct
     4681 tccgaaacta taaggctcac aagccttgcg gatcttgccg acctaagata acattaattt
     4741 aataattttg cagcgaggag cattccgctt ctcgctgcaa ttgtgaaaat gcgtttaaaa
     4801 tataaatttg cttagtttat ttttttgatt ttagggaata attatagtta ctttaatggt
     4861 ttttgtgtaa gtaacttcca gcattttaga caatatgcga attctaaaga atgtagttga
     4921 catcatttgc attcttgtga taagattttg tattgtgcag aagaagtttg caaaaaatgt
     4981 cacctatctt aatcatttcg gttttaaggt tatgccttga aatatattaa atcacgcaag
     5041 gaggtgcaag gtttgccaac ctttaatcag ctggttcgtc acggtagaca atccgtggag
     5101 aaaaagtcca agtccccggc actcggcaag agtcttaact ctcagaagaa cattcttctt
     5161 gaccagaact ctccccagaa gagaggtgtc tgcacagtag ttaagaccga tactcccaag
     5221 aagcctaact cagctctcag aaagatcgcc agagtaaggc tcaccatcgg aatagaagtt
     5281 acggcttata ttcccggtga aggtcacaac cttcaggagc acagcgtagt gcttatcagg
     5341 ggcggccgtg ttaaggacct ccccggcgtt cgctatcaca tcatccgcgg cacatacgat
     5401 actcagggtg ttaacggcag aatgcagggt cgttccaagt acggcaccaa aaaggcaaag
     5461 gctaagaaat agccgcatta tcgggacaat tcttcttgca ggctgaatta aattcaccaa
     5521 tgcgtgttta taatatatta caagtcctct ttgtaagtta aactgcattg gcgccgacgg
     5581 gaagtatgtc actcgagtac ctgtgatatc atttcattat gaaggaggga agcgaagtgc
     5641 caagaagagg caaggttgca aaacgagatg ttttgcctga tccgctttac aattccaaac
     5701 ttgtaacaaa gctcatcaac aatgttatgt atgatggcaa gaaaggcgtt gctcagaaga
     5761 tagtttacgg cgcctttgac atcatcagag aaaagacagg caaggagccg cttgaggtct
     5821 ttgaggccgc tatggagaat gtcatgccgc tgcttgaggt taaggctcgc cgcgtaggcg
     5881 gtgctaccta tcaagtaccg atggatgttc gtccggagag gcgtcagaca ctgggtctca
     5941 ggtggatcgt aacttatgcc cgccagcgtt ctgaacgcac catgaaggaa agacttgctc
     6001 acgaaatcat ggatgccgtt aactcaacgg gcggcgcatt caagaagcgt gaagacaccc
     6061 acaagatggc cgaggccaac aaggcttttg ctcactaccg ctggtaatca cgctatttat
     6121 aagtatattt ggaggttttt atgccaaggc aggttccgct ggatttaaca cgaaacattg
     6181 gaataatggc acatattgat gccggaaaaa cgaccacaac cgaaagaatc cttttctata
     6241 ctggcgttaa ccataagatg ggcgagaccc acgatggttc cgccactatg gactggatgg
     6301 ctcaggagca ggagagaggt attaccatca cttctgccgc taccacatgc ttttggaaag
     6361 gtcacagaat caatatcata gacacaccag gccatgttga cttcacagtt gaggtcgaaa
     6421 ggtcccttcg tgtccttgac ggctctgtaa cggtcttatg tgccaagggc ggagtcgagc
     6481 ctcagtctga aactgtttgg cgtcaggcgg acagatacaa ggttccgaga ctggcgtatg
     6541 taaataagat ggacatcatg ggagcagatt tctataaagt tgtcgacatg atgaaggaaa
     6601 ggcttaagtg caacgccgtt ccaattcagc ttcccatagg ttctgaaagc agtttcagag
     6661 gcgtaataga cctcgttgaa atgaacgcta ccgtatacta tgacgatttg ggccttaata
     6721 tcaaagttga ggaaatcccc gaagattata aagagctcgc agataagtac aggattgagc
     6781 ttttagagca tgtggcagag caggacgacg aactcctcga aaaatacctt tcgggagaag
     6841 agctcactat tgaagaaatc aaggcatgca tcagaaaggc taccattaat aatgacatgg
     6901 tgcccgtggt ttgcggtaca tcctacaaga acaagggcgt acagaagctc cttgacgcga
     6961 tagtcgatta tctgcccgct ccgacagatg ttgagtcaat caggggcatc aaccccgaca
     7021 cccaggagga agaggtaagg cattcctccg acaacgagcc cttctcagcg cttgctttca
     7081 agattgctac cgacccctat gtaggaaggc tttgcttctt ccgcgtatat tcgggttcag
     7141 taacatcggg cagcactgtt tacaactcaa ccaagggcaa aaacgaaaga atgggcagaa
     7201 tcctgcagat gcactccaac cacagacagg acatagaggt atgctacgca ggtgacatcg
     7261 ccgcggttgt aggcgttaag ttcaccacaa cgggcgacac cctttgcgat gagcgccacc
     7321 cgatcatcct tgagtccatg gagttccccg agcccgttat ccgcgtcgcc attgagccta
     7381 agacaaaggc aggccaggag aaaatgggca tcgcgctgca gaagctctct gaggaagacc
     7441 cgactttcaa atactacacc gaccccgaaa caggtcagac aatcatcgct ggcatgggcg
     7501 agcttcatct tgagattatt gtcgaccgta tgctgcgcga gttcaaggtc gaggccaatg
     7561 ttggcagacc ccaggtcgca tacagagaaa caatcaccaa agaagctatt tccgatacca
     7621 agtatgcaag gcagacaggc ggtcacggtc agtacggcca tgttaagatc aagattgagc
     7681 ccaacccggg caaaggcttt gagtttgtca acgcgattgt gggcggcgta attcccaagg
     7741 agtatatccc cgcagttgag gaaggcatta agggagcgat gcagacgggc gtcctcgcag
     7801 gctacaatgt agttgatgtt aaggtcacgc ttttcgacgg ttcttcccat gaagtcgact
     7861 cctccgaaat ggcattcaag attgccgctt caatggcatt caaagatgcc atgaagaagg
     7921 caggccctgt tctcatggag cccatcatga agattaccgt cattgttccc gatgagtata
     7981 tcggcgatgt tatcggcgac atcaatgccc gccgcggtga gatgcgcaag atggaaacaa
     8041 gagtgggagc aactcatgtt catgcgttcg ttccgctttc atccatgttc ggctatgcaa
     8101 ccgatttgcg ttcaaagacg cagggcagag gacagtatgt attggagccg agccactaca
     8161 cagaagtgcc caaatccatc gctgaatcaa tcatcggtga aaggttcaag aatttataaa
     8221 taaatgcaaa tcaagtaata aagtacttga aattaatggt ttttattgat agaatagcga
     8281 ataataggat taacgacttt accttaagga ggaaattcga aaatggcaaa agctaaattt
     8341 gacagaacga aaccccatgt aaatatcggc accatcggcc atgttgacca tggtaagaca
     8401 acattaacag ctgctatcac aaaggcactt gctcttaagg gtacctccca gttcgttgac
     8461 tatgccaaca tcgacaaggc tcccgaggag aaagagcgcg gtatcacaat caacacttca
     8521 catgttgagt atcagaccga aacccgtcac tatgctcatg ttgactgccc cggccacgct
     8581 gactacatta agaacatgat caccggcgca gctcagatgg acggcgctat ccttgttatc
     8641 gcagctacag acggcccgat ggcacagaca aaagagcacc ttctccttgc tcgtcaggtt
     8701 ggcgttccct acatcgttgt tttcatgaac aaggttgacc aggttgacga ccccgagctt
     8761 cttgacctcg tagagatgga aatccgcgag actctcgacg cttatgagtt ccccggcgat
     8821 gatacaccca tcatcagagg ttcagctctc caggctcttg agtacaacgg caatgacccc
     8881 aacgctcccg agctcaagcc catctatgag cttatggacg ctgttgacag ctatatcccg
     8941 acacctgacc gtaaatccga catgcccttc cttatgcctg ttgaagacgt cttcacaatc
     9001 acaggccgcg gcacagttgc tacaggtaga gttgagcgcg gacagctcaa gatcggtgag
     9061 gaagttgaga tcgttggtct tgctgatgag agtagaaaag ttgttgttac cggtatcgaa
     9121 atgttccgta agatccttga ctatgcagag gctggcgaca acatcggcgc actcctccgc
     9181 ggtgttcaga gaaacgaaat cgagcgcgga caagttcttg caaagcccgg ctccattaag
     9241 cctcacacca agttcaaggg tcaggtttac gttcttacca aggatgaagg cggccgtcat
     9301 aagcccttct tctccaacta tcgtccccag ttctatttca gaacaactga tgttaccggc
     9361 gtaattcagc tccccgaagg cgttgaaatg tgcatgcctg gtgacaacgt cgttatgagc
     9421 gttgagctta tttcacccat cgctatcgag gaaggtctcc gcttcgctat ccgtgaaggc
     9481 ggccgtacag ttggctccgg cgttgttatc gaagtcggtg attaattaaa tctaaaacaa
     9541 attaagcggt tcgtaatgaa ccgctttttt gtttgcctaa at
//
LOCUS       DAONVQ010000079         9422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5586_length_9422_cov_4.314829, whole genome shotgun sequence.
ACCESSION   DAONVQ010000079 DAONVQ010000000
VERSION     DAONVQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9422
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5586_length_9422_cov_4.314829"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLD98_05990"
     CDS             complement(<1..268)
                     /locus_tag="PLD98_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05833.1"
                     /translation="MLEIISVYYKKITAFLVSMLMLFATSLLGDIPRAKNPIVAEKTN
                     YVFDTDRILIGGYGCNISQTDDEHMQYVVDAGIDFLVSAANKEFL"
     gene            complement(465..1187)
                     /locus_tag="PLD98_05995"
     CDS             complement(465..1187)
                     /locus_tag="PLD98_05995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQA05834.1"
                     /translation="MEDNKKSRVLEILKTKPIYPVLALCLIAIGVAAYASNKGLPAEN
                     EGVTPLTPTTEARTEVPTDEVRGDVTGMPDERKQTTAVPTTEAKTEPKTEPETKPTYF
                     SLPLGTAIAFDYSHGELVYSKTMNDWRVHNGVDFSGKVGDSVKAIADGVVEAVFDDEL
                     WGTVVVIDHGGGVMAKYCGFGKGSTVKKGDKVKLNDTIGNLGTVPCEEALGPHIHLEI
                     AVNGEIKDPLSVLGKDKSPVND"
     gene            complement(1508..3238)
                     /locus_tag="PLD98_06000"
     CDS             complement(1508..3238)
                     /locus_tag="PLD98_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05835.1"
                     /translation="MKLLLKLSREAVKYRRLYFIAISSTFLLTAVNLIAPKLLSSLTE
                     LVKNGEAGKNLKALAFLTGGLFGLYLLMVLFRFMSNYFAHKAAWSLVEEMRVRVYDKI
                     QTSSISFFHDKQTGDLMSRVVNDTATFELLYAHVIPDMMTSVITVVGVFAILLSINVK
                     LALLTCIPIPFLFWAGMTFAFKIRPNFRLSQKQLAALNSKLQDNFSGIHEIQAFGREK
                     DEQESVLEQAHSYTKAILFALKKSALFHPGIEFISSMGTVIVVGAGGLLAFKNILSVS
                     DIVAFLLYLSLFYAPISKFARLLEDSQQAYAGAERVMAILEKPQEICDKENAVDIGKV
                     KGEIEFKNVSFHYQEGITVLDDISFKVKPGQMVALVGPTGAGKTTLTQLIPRFYDVIS
                     GSILIDGVDIRDAKLESLRRNIAPVLQDTFLFNATVAENISYAKPDATMEEIVAAAKA
                     AFIYDDIMEMPDGFDTKVGERGLRLSGGQKQRIAIARAILRDAPIIILDEATASVDSR
                     TEREIQKAISSLLGKRTIIAIAHRLSTIRNADLILVLENGKITECGTHEELMQNHGLY
                     QKLNLIDKEE"
     gene            complement(3341..3739)
                     /locus_tag="PLD98_06005"
     CDS             complement(3341..3739)
                     /locus_tag="PLD98_06005"
                     /inference="COORDINATES: protein motif:HMM:NF016757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PT domain-containing protein"
                     /protein_id="HQA05836.1"
                     /translation="MKKFNKVLALLLALLMAFSAFGVIAMAEDVDPTEETTEVEETTK
                     NTIVVGSWKELKFAVAFKLLEKVFMWLEKIFKDLFEKIMPPAAEEPTTVEPTEEPTEE
                     PTEEPTEELTEEPTEEPTEEETTEELSTAA"
     gene            complement(3877..4464)
                     /locus_tag="PLD98_06010"
     CDS             complement(3877..4464)
                     /locus_tag="PLD98_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HQA05837.1"
                     /translation="MKEKAVSKNKAASIYKIAAAAIFAALIAVGAFIKVPMYPVPITM
                     QMLFTFLAVLLLGKWGGTVSVLIYILIGLLGLPVFSFGGGLTSFLKPSFGYILGFAAG
                     NFLSGLFIDKLRIKSVKGYFAALTLNSLIIYIIGVTYLYLIMTLYLQKNVAATTLIVD
                     YFLIFIPGDVLKALAASFIAFRVKPVTDKFQKRNS"
     gene            complement(4521..5282)
                     /locus_tag="PLD98_06015"
     CDS             complement(4521..5282)
                     /locus_tag="PLD98_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05838.1"
                     /translation="MEKRQNAASRRATASAYVIFCITVWFLAEVWFLIKKATGIADSE
                     NQLLSIIVGAVGFLGGIAYISTPIFLFDAFSKEKKANIEEKGKGGIFSILNPFLIIMI
                     VFAVLALIGLFITKAVDLASEQGVDSGFSDKTIFLAKFSLVLAGIFDLQILFALYSFG
                     IYKYETAEKRGGEAVAKLAFMAAVLSFLCYVVEVYNIFIEFLTYFNRTSAQPLPKLFT
                     NAVAILWAGFIIALHLKRRSILKPKKNKADEAYEQ"
     gene            complement(5303..6427)
                     /locus_tag="PLD98_06020"
     CDS             complement(5303..6427)
                     /locus_tag="PLD98_06020"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HQA05839.1"
                     /translation="MNSTPFNPAEIIEIYDIRHKYLGYHPLCSGHINDTYVLEFQTDE
                     GSKHSLLLQRINTNVFKKPVELMQNIVAVTSFLREKIIEEGGDPERETLNVIRTKDGD
                     FLFVDKAGHYWRLYNYIENAYSVDIPEDPKLFYELGKAFGKFQKMLDSFPIDTLHETI
                     PDFHNTKKRYEAFEKAVEENLSGRLENALPEIEFVRARKADASVLVDMIAEGKLPLRV
                     THNDTKLNNVMFDSESHKSICVVDLDTVMPGLSLYDFGDSIRFGANTAAEDEKDVSKV
                     SLSLPLYTEYVKGYLSEAGNILTPAEVEYLPFSAKLMTYECGMRFLTDFLNGDTYFKT
                     KYPEHNLVRCRTQFALVADMERKMDEMQKITQEEYKKVTS"
     gene            6846..7553
                     /gene="pyrH"
                     /locus_tag="PLD98_06025"
     CDS             6846..7553
                     /gene="pyrH"
                     /locus_tag="PLD98_06025"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQA05840.1"
                     /translation="MEPFYDRVILKLSGEVLAGENGFGYDFEMLDRISLAIGECKELG
                     TQIGIVVGGGNFWRGRSCGEMHRASADNVGMLATAMNAIVLSDALYQNGINNRVMSAI
                     FMPKICEHYVVEKARDYMKNVGVVIFACGTGNPFFSTDSAAALRAAELDAKVVLKGTN
                     VDGVYDKDPRKHPDAVRFDCITHDEVLARGLGIMDAAATAICKENDINTIVFDLTKPE
                     NIVSAVKGICIGTIVTK"
     gene            7620..8141
                     /gene="frr"
                     /locus_tag="PLD98_06030"
     CDS             7620..8141
                     /gene="frr"
                     /locus_tag="PLD98_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQA05841.1"
                     /translation="MTKTLSVLKSDFDAIRAGRANAAVLDKIYVDYYGSPMPINQLAT
                     ITVAEARVLTIQPWDSSVLSLIEKAIQSSDLGINPQNNGRIIRLVFPQPTEERRKQLV
                     KDVSKMGEDAKIAIRQVRRDAIEKLKSMKKNSEITEDDLKYGESEVQKITDEFVKKVD
                     DAVAAKEKEIMEI"
     gene            8210..8944
                     /locus_tag="PLD98_06035"
     CDS             8210..8944
                     /locus_tag="PLD98_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HQA05842.1"
                     /translation="MANSEKFKDIVLPRHVGVIMDGNGRWAKKRGLPRYEGHREGAKT
                     FRRIAEYASEIGIKNITFYAFSSENWKRPPQEVNAIMQLLREYLDEAERRQKENEEKG
                     IRIRFIGDLSRLPEDIRKKIEEAQKLSEEKTKCTVSIAVNYGGRQEIVKAVRDIAEKA
                     RKGEIKPEDITEADIENCLYTAGIPDADLIIRPSGEKRLSNFLTWQSVYAEFYYTDVL
                     WPDFTTDDFDKAILDFANRDRRFGGV"
     gene            9055..>9422
                     /locus_tag="PLD98_06040"
     CDS             9055..>9422
                     /locus_tag="PLD98_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05843.1"
                     /translation="MKQRIITGLIAGAFFISMMLALYTPIFPLFVCLLSGVAVFEINK
                     VMGLKYKPLKFLSILTASLLPLAITYSQFNSFFPFLIIYTVLMLSWMLGDYEHTKFVT
                     VASSVFVSIALPFALSTMVL"
BASE COUNT         2567 a         2150 c         2208 g         2497 t
ORIGIN      
        1 caaggaactc tttattcgcg gcgctgacaa gaaagtctat ccccgcgtcg acaacatact
       61 gcatatgctc gtcgtcggtc tggcttatgt tgcagccgta gccgcctata agaatgcggt
      121 cggtgtcaaa gacatagttt gttttctcag ccacaatggg gtttttagcc cgggggatgt
      181 cgcctaaaag ggatgtggcg aaaagcatca gcatgcttac gagaaaagcg gtaatctttt
      241 tataatagac gcttataatc tcaagcatat tttcccctcc attagtttct tggacgagca
      301 tattgtagca tagtgaaaca tcgaattcca caaaaaattg cggttatgta aaaaatttaa
      361 acttttccga aaagcggata cggaaaaagc gcaaaaaaaa agcggctgac tttcagccgc
      421 cgcagccccg aaaactcggt gcgggttaca actcagggct aactttaatc atttacggga
      481 gacttgtctt tccccagaac cgaaagggga tcctttattt cgccgtttac cgcaatttca
      541 aggtgaatgt ggggtcccag agcctcttcg caagggacgg tgcccagatt gcctattgtg
      601 tcgttgagct ttaccttgtc gcctttcttg acggtgctgc ctttgccgaa gccgcagtat
      661 tttgccatta ctccgccgcc gtggtcaatc acaacaacgg tgccccaaag ctcatcgtca
      721 aagacagctt caacaacgcc gtcggcaatt gcctttacgg agtcgccgac cttgccgctg
      781 aagtccacgc cgttgtgtac tctccagtcg ttcatggtct tggaatagac cagctcaccg
      841 tggctgtagt caaaggctat tgcggtgcca agggggagag agaagtaggt gggctttgtc
      901 tcaggctctg ttttgggctc tgttttagcc tcggtggtag gtactgcagt agtctgcttt
      961 ctttcgtcgg gcatgcctgt tacatcgccg cggacctcat cggtcggtac ctcggttctt
     1021 gcctcggtgg tgggagtaag gggagttacg ccctcattct cggcgggcag acccttgttc
     1081 gaggcataag ccgcgacgcc gatcgctata aggcaaaggg ccaaaaccgg atatataggc
     1141 tttgttttaa ggatttcaag caccctgctt tttttgttgt cctccataat caagaataac
     1201 acctccaaaa gaatttttgc ccaggatatg caaattattc aaggaagtga aatttttgaa
     1261 atcggaggac aaaaaaacaa acaaaagcag acctttcagt ctgcttttgt ttagctgttg
     1321 tttaacaaaa gaggggttac taataggagg gatatcttcc atcgaagaat gtgtagatac
     1381 tgcgggtgct ggtgcggctc cgctcctcgc tcgctacacc tttatgatat cctcaacata
     1441 ttaagcggct atgaacatcc tattaataat cggaaattta attattaata catattgtcg
     1501 aattttgtta ttcctctttg tcgataaggt ttagtttctg gtacagtccg tggttttgca
     1561 tcagctcttc atgggtgccg cactcggtga ttttgccgtt ttcaagaacc agaatcaaat
     1621 cagcgtttct gattgtggaa agcctgtggg ctatggcaat aatggtgcgc ttgccgagaa
     1681 gggatgagat agccttttga atctcccttt cggtgcggga atccaccgac gccgtagcct
     1741 cgtcaagaat gataatcggc gcgtctcgga gtattgccct cgctatggcg attctctgct
     1801 tttgacctcc cgagagcctc agacctcttt cgcccacctt agtgtcaaag ccgtcgggca
     1861 tctccatgat gtcgtcatat ataaaggctg ccttggcggc ggcgactatt tcctccatgg
     1921 tggcgtcggg ctttgcgtag cttatgttct cggctaccgt ggcattgaaa aggaaggtgt
     1981 cctgtaaaac gggagcgatg ttgcgcctta aagactccag ctttgcatcc cttatgtcga
     2041 cgccgtcaat aagtatgctg cccgatatta catcatagaa gcggggaata agctgcgtca
     2101 gagtcgtctt gccggctccc gtggggccta caagagccac catctgaccc ggctttacct
     2161 taaagctgat atcgtcaagg accgtaatgc cctcctgata gtggaagctg acattcttaa
     2221 actcaatctc gcccttgact ttgccgatgt ctactgcgtt ctccttgtcg cagatttcct
     2281 ggggcttttc aaggattgcc ataactctct cggcgcccgc gtacgcctgc tgagagtcct
     2341 ctaagagcct tgcgaacttt gaaataggtg catagaaaag ggagaggtac aaaaggaagg
     2401 cgactatatc cgaaacggaa aggatatttt taaaagccag aagtccgccc gctccaacaa
     2461 ctatgaccgt acccattgag gaaataaact caatgccggg atggaaaagc gcgctttttt
     2521 taagagcaaa gagaatagcc tttgtataag aatgagcctg ctcaaggaca gattcctgct
     2581 catccttttc cctgccgaac gcctgaattt cgtgaattcc cgaaaaattg tcctgcagct
     2641 tagagttcag agccgcaagc tgcttttggg agagtctgaa attcggcctt atcttaaagg
     2701 caaaggtcat gcccgcccag aaaaggaagg gtatcggtat gcatgttaaa agcgccagtt
     2761 ttacatttat gctaaggagg attgcaaaga cgcccaccac tgtaataacg ctggtcatca
     2821 tgtcgggtat tacatgggcg tacaagagct caaaagtggc ggtgtcgttt acaactcggc
     2881 tcataaggtc gcccgtctgc ttgtcgtgga aaaagcttat ggaggaggtc tgaatcttgt
     2941 catagaccct gactctcatt tcctcaacaa ggctccacgc ggctttgtgg gcaaagtagt
     3001 tgctcatgaa tctaaagagc accataagaa gatacagccc gaaaagaccg cctgtcaaaa
     3061 acgccagcgc tttaaggttc ttgcccgcct ctccgttctt aacaagctcg gtaaggcttg
     3121 agagcagctt cggggcgata agatttaccg ctgtcagcaa aaatgtggag ctgatggcga
     3181 taaagtagag tctgcggtat ttcaccgcct cgcggctaag tttaagaagt aatttcatcg
     3241 cgtttctcca atccgaattc aaaagtgaat tcaaagcggc gaaccttcgg ttcgccgcct
     3301 ataagctgta tttaattgat tatgtactct ctgtttgaat ttaagcggca gtagacagct
     3361 cctcagtggt ttcttcctct gtgggctcct ctgtcggttc ttctgtaagc tcctctgtag
     3421 gttcctctgt aggctcctct gtaggctcct cagtgggctc tacggttgtg ggttcctctg
     3481 ctgcgggagg cataatcttc tcaaagaggt ccttgaagat tttctcaagc cacatgaaaa
     3541 ccttctctaa gagcttgaaa gctactgcga atttaagctc cttccatgag ccgacgacga
     3601 ttgtgttctt tgtggtttcc tcaacttctg ttgtttcttc tgtggggtca acatcttcag
     3661 ccattgctat tacgccgaaa gctgaaaaag ccatcagcaa agcaagaagc agagcaagta
     3721 ctttgttaaa ctttttcata ctgatcctcc taattaaatt ttcattttcc aatttatgaa
     3781 accgttactg ccacaattat atataaatct ttacatcaag tcaaccaaat ttggtctaaa
     3841 acaagataaa aaacagtgaa aagtgatgac aattttctat gaatttcgtt tttgaaattt
     3901 gtccgtaacg ggctttactc taaaggctat gaatgacgcc gccaaagctt ttaagacatc
     3961 cccgggtatg aatataagga agtaatccac gatgagtgtg gtggcggcaa cattcttttg
     4021 aagatacaaa gtcataatca agtacaaata tgttacgccg attatgtata ttatgaggga
     4081 gttaagcgtc aaagcggcaa aatagccctt gacagatttt atgcggagct tgtctataaa
     4141 aagccccgat aggaaattgc ccgccgcgaa gcctaagatg tatccgaatg acggctttaa
     4201 aaagctcgtc agaccaccgc cgaaagagaa aacgggcaga cccaaaagtc cgatgagaat
     4261 ataaattaac accgatacgg ttccgcccca tttgcccaga agcagaacgg caaggaaggt
     4321 gaacagcatc tgcatggtta tgggaaccgg gtacatggga acttttataa acgcgcccac
     4381 ggcgataaga gccgcaaaaa tcgccgcggc ggctattttg tatatgcttg ccgctttatt
     4441 ttttgaaact gctttctcct tcataaacgc cccctttaaa ttatcagaac cccttaaatt
     4501 aaggggttcg ggaatttctc ttactgctcg tacgcttcgt ccgctttgtt tttctttggc
     4561 tttaaaatac ttcgcctttt caagtgcagg gcgatgataa atcccgccca aagaatagcc
     4621 acagcgtttg taaagagttt gggcagcggc tgggcggatg tccggttgaa gtatgttaaa
     4681 aattcaataa atatgttgta gacttccact acataacata aaaaggacag aactgccgcc
     4741 atgaacgcca gtttcgccac agcctcaccg cctcttttct cggcggtttc atatttgtaa
     4801 atcccgaatg agtacagcgc aaaaagaatt tgaaggtcga agattcccgc aagcaccaac
     4861 gaaaacttcg cgaggaagat ggtcttatcg ctgaatcccg agtcaacccc ttgctctgac
     4921 gctaagtcta cggctttggt aataaaaagt cctatcagcg cgagcacggc aaatacaatc
     4981 atgataatta agaaaggatt aagaatgctg aatatgccgc ccttgccttt ttcttcaata
     5041 ttagctttct tttctttgct gaaggcatcg aacaaaaaga taggcgtaga gatataggca
     5101 ataccgccta agaaacctac agcgccgaca attatggata acaattggtt ttcggagtca
     5161 gcgatgcccg ttgctttttt gataaggaac catacttcgg ccaaaaacca tacggttatg
     5221 cagaatatga catatgctga cgcggtggct ctgcgggagg ctgcgttttg tcttttttcc
     5281 ataccctcac cttttcaata atttagcttg tgactttctt atactcctcc tgcgtgattt
     5341 tttgcatctc gtccattttt ctttccatgt cggcaacaag ggcaaactga gtcctgcatc
     5401 tgaccaaatt gtgctcgggg tactttgtct taaaatatgt atcgccgttt aagaagtctg
     5461 ttaaaaagcg cattccgcac tcataggtca taagtttagc ggagaagggc agatattcaa
     5521 cttcagcggg ggtgaggata ttgcccgcct ccgaaaggta gccttttaca tactccgtat
     5581 aaagggggag gctgagggat accttagaga catccttttc atcctctgcg gctgtgttag
     5641 cgccgaatct tatgctgtcg ccgaaatcgt atagggaaag tccgggcata accgtgtcaa
     5701 ggtcaacaac gcaaatgctc ttgtgagatt ccgaatcgaa cattacattg ttgagctttg
     5761 tgtcattgtg ggtaactcta agcggtagct taccctccgc aatcatgtca acaagtacgg
     5821 atgcgtcagc ttttcttgcc ctaacgaatt caatttcggg gagggcgttt tcgagcctgc
     5881 ccgagaggtt ttcctccacc gccttctcaa aagcctcata tctctttttt gtgttatgaa
     5941 aatcgggtat ggtctcatga agtgtgtcaa tggggaaaga gtcaagcatc ttctggaact
     6001 tgccgaatgc tttgcccagt tcgtaaaaga gtttgggatc ttccggaatg tcaacggaat
     6061 aggcgttttc aatatagtta taaagcctcc agtaatgacc tgctttgtcc acaaaaagga
     6121 aatccccgtc ttttgtgcgt attacattca gtgtctccct ttcggggtcg ccgccctcct
     6181 cgataatctt ctctcttaaa aacgaggtta ccgcgactat attctgcata agctcaacgg
     6241 gctttttgaa tacattggtg ttaatacgct gcaaaagaag ggaatgtttt gagccttcgt
     6301 ctgtttggaa ttcgagtaca tatgtatcgt ttatatgtcc gctgcacaga gggtgatagc
     6361 ccaggtattt gtgccttata tcatagattt caataatttc cgcagggtta aagggtgtgc
     6421 tattcatagg aattcagcct ttcgattatt cttgcggtga tgccgcctgt aactaagatt
     6481 gctaaagaag gaaaattgtt gaaatttttt taaaacaata aacaatcaag aagccttccc
     6541 gccccaagtg ggggagatgc ggcatcttga ctttccactg cctaagattt catgattcat
     6601 ttggcaaaaa tgacaaaaaa atttaattgt ttacagtgag cattatatac aatattggca
     6661 aattagtcaa taatattgtt gttatcccct ttacacaatt gattaaatga agtaaaataa
     6721 gtccgtactg attcagccgc caaaaattta ctttgtgaat tcatgaatat tcactaatta
     6781 aagatttatg cgttaaaaaa gcggaaaccg aaagcggcgg aaaggaaaac tttggagggg
     6841 atagtgtgga accattttac gatagagtta tcctgaaact tagcggagag gttttggcag
     6901 gggaaaacgg cttcggctat gactttgaaa tgctcgacag aatcagcctt gccatcggag
     6961 agtgcaagga gctcggcact cagatcggca tagtcgtcgg cggaggaaac ttctggagag
     7021 gacgctcctg cggagaaatg cacagggcaa gcgccgacaa tgtcggtatg ctggcaacgg
     7081 caatgaacgc aatcgtgctg tccgacgcct tatatcagaa cggcattaac aacagggtca
     7141 tgtcggcaat ctttatgccc aaaatctgcg agcactatgt tgttgaaaag gcaagggact
     7201 atatgaagaa tgtgggcgtt gtaatattcg cctgcggaac cggcaatccc ttcttcagca
     7261 ccgactccgc ggcagccctc agagcagcgg aacttgacgc aaaggttgtc ttaaagggca
     7321 caaatgttga cggcgtctat gacaaggacc cgagaaagca ccccgacgcg gtaaggttcg
     7381 actgcattac ccatgacgag gtattggcaa ggggtctcgg cataatggac gctgcggcaa
     7441 cggccatctg caaggaaaac gacataaaca ccatagtctt tgacttgaca aaacccgaga
     7501 atattgtttc agccgtaaaa ggcatttgca ttggcaccat tgtcacaaaa taaaacaata
     7561 ttaatttcgt caaaggagga tataccgtgg agaccgtatt taatactgcg aaagagaaaa
     7621 tgacaaagac tctcagtgta cttaaatctg atttcgacgc aattcgtgcc ggcagagcaa
     7681 atgccgcagt gcttgacaaa atatatgttg actattacgg ttcccccatg cctataaatc
     7741 agcttgcgac catcacggtt gcggaggcaa gggtacttac cattcagccc tgggattcca
     7801 gcgtgctgtc tttaatagaa aaggctattc agtcctctga ccttggcatc aacccccaga
     7861 acaacggcag aatcattcgc cttgtattcc cccagcccac agaggagcgc agaaagcagc
     7921 ttgtcaagga tgtcagcaaa atgggcgagg acgcaaagat agccattaga caggtaaggc
     7981 gcgacgccat tgaaaagctg aaatccatga agaagaattc cgaaatcacc gaggacgacc
     8041 tgaaatacgg tgagagcgag gtacagaaaa tcaccgacga gttcgtaaag aaggttgacg
     8101 acgcggtcgc ggcaaaagaa aaggaaataa tggaaatata aaccctctaa tgcctgcctt
     8161 tttaaaagtc aggcattaca gcttttatag gaaaaaacgg aggctataaa tggcgaacag
     8221 cgaaaagttt aaagatattg ttcttccccg ccatgtgggc gtaatcatgg acggcaacgg
     8281 ccgctgggca aagaaaagag gccttccaag atacgagggt cacagggagg gcgcaaagac
     8341 tttcagaagg attgccgagt acgcctcaga aatcggtata aagaacatca ccttctacgc
     8401 cttctccagc gaaaactgga aaagaccccc tcaagaggta aacgccataa tgcagctttt
     8461 gagagaatac cttgacgagg cggagagaag gcagaaggag aatgaagaga agggcatcag
     8521 aatacgcttt ataggtgacc tttcccgcct gcctgaggat ataaggaaga aaatcgaaga
     8581 agcccagaag ctcagcgagg aaaaaacaaa atgcaccgtt tcaatagccg taaactacgg
     8641 cggcaggcag gagatagtga aggctgtgcg ggacatagcc gagaaagcgc ggaaaggcga
     8701 aattaagcct gaggacatca ccgaagccga tattgaaaac tgcctttata ccgcaggcat
     8761 tcccgacgcg gatttgatta tccgccccag cggtgaaaag aggctctcca atttccttac
     8821 atggcagtct gtttacgcag agttttacta caccgatgtg ctctggccgg atttcaccac
     8881 agacgacttt gacaaggcga ttctagactt tgccaaccgt gacaggcgtt tcggcggggt
     8941 ataaaacttt ctgcggctta gctattaagt tactgaaata tacaacttca agacttaaga
     9001 taaaaaacat gggagcgccc gagggcattc tctcacactg aaaggaagat atttatgaaa
     9061 cagcggatta tcacaggtct aatagcggga gccttcttta tcagcatgat gctcgccctc
     9121 tatactccta ttttcccgct ctttgtatgc cttttgtcgg gcgtggccgt gtttgaaata
     9181 aacaaggtta tgggccttaa gtataaaccc ttaaaatttt taagcatcct caccgcctct
     9241 ttactgcctt tggcaattac ttattctcag ttcaacagtt tcttcccgtt tttaatcata
     9301 tatacggtac tgatgctttc atggatgctg ggcgactatg agcacacaaa gttcgtcacc
     9361 gtcgcatcat ccgtattcgt atcgatagcc ttaccctttg ccctttccac catggtgctt
     9421 tt
//
LOCUS       DAONVQ010000080         9397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5601_length_9397_cov_4.197602, whole genome shotgun sequence.
ACCESSION   DAONVQ010000080 DAONVQ010000000
VERSION     DAONVQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5601_length_9397_cov_4.197602"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLD98_06045"
     CDS             <1..461
                     /locus_tag="PLD98_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HQA05844.1"
                     /translation="IVTNGDQTDTIYDYLSNGRTFEEALNIRSFEPDTPNYTPRISGI
                     MHINEGAEFKLSILKSADGNAASCNRFVYSYDGGLGGGRFIHTYMGDSTPLRSFEGEP
                     WFVELSNDIDETTKDIWSSLNEENKISLFVRYIGIYTGFSETRIVNKHLK"
     gene            475..1656
                     /locus_tag="PLD98_06050"
     CDS             475..1656
                     /locus_tag="PLD98_06050"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HQA05845.1"
                     /translation="MDFIELKYGCNPHQKPSKIYVDSGKLPLEVLNGTPGYINFLDAL
                     NGYQLVKELKEATGLPAAASFKHVSPAGAAVGLPLDDTLKKMYFCEDLEGLNDSPIAC
                     AYARARGTDRMSSFGDFVALSDTCDLTTAKLLKSEVSDGIIAPDYTDEALEILKAKKR
                     GTYAIIKIDPNYVPEELEVRQVYGIKFLQGRNNSVIDKSLLENIVTKNKNIPDSAKTD
                     LLIALITLKYTQSNSVCYAFGGQTIGVGAGQQSRIHCTRLAGDKADVWQLRRHEKVQN
                     LKFLPTLKRADRDNLIDIYITEGLSSILKNSDWRNYFAECPEELTSEEKRSYLDSLKG
                     VSLGSDAFFPFSDNIERAYRSGVSFVAQPGGSVNDSAVIEACDKYGMVMAFTGLRLFH
                     H"
     gene            1676..2959
                     /gene="purD"
                     /locus_tag="PLD98_06055"
     CDS             1676..2959
                     /gene="purD"
                     /locus_tag="PLD98_06055"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQA05846.1"
                     /translation="MNILVVGSGGREHAIIKKLKEGRPDLNIFAVPGNGGIASDAECV
                     PEISAKDIDKIADFAEKNKIDFAVVALDDPLVLGAVDKLHEKGIPCFGPNKKAARIEG
                     SKVFAKNLMQKYGIPTAKYSVFESADEALGFLNATEFPVVVKADGLALGKGVFICPDR
                     DTAEKAIKEIMLNKIFGESGNKIVIEEYLTGPEVSVLCFTDGKTIIPMVSSMDHKRAL
                     DGDKGFNTGGMGTIAPNPYFTEEVAKECMEKIFIPTINALNAEDSKFKGCLYFGLMIT
                     KDGPKVIEYNCRFGDPETQVVLPLLKTDLLSIMLATENECLDSIDVEFYDKSACCVVI
                     ASGGYPEKYETGFEIEIDEKSLPENISIFQAGTKLRNGKLVTSSGRVLGVTAVADTLK
                     QAIIDAYKACSLISFTNCFYRKDIGQKALEIINKK"
     gene            2979..6683
                     /locus_tag="PLD98_06060"
     CDS             2979..6683
                     /locus_tag="PLD98_06060"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HQA05847.1"
                     /translation="MVYRVYVEKKKGLAHEAAALLNDIRTNLGVTSLKNIRIFNRYDV
                     ENISKELFDDAISAVFSEPMVDDVYYELPNLEDAYIFAVEFLPGQYDQRADSAAQCIQ
                     LISQKDRPLVRTAKVYALFGELTESEIGAIKKHLINPVESREAALELPQTLNLDYDIP
                     QTVETLDGFIDLKDEDIKEFVKKYALAMDEDDLKFCIEYFKSEGRNPTITELRVLDTY
                     WSDHCRHTTFLTTIDSVEIDDAEIKSAYDLYLTLRAETGRADKKINLMDIATIGARYL
                     KKQGLLEKLDESDEINACTVKIDVDIDGENEEWLLLFKNETHNHPTEIEPFGGAATCI
                     GGAIRDPLSGRAYVYQAMRVTGAGNPLKPLSETIEGKLPQRKIVTTAADGYSSYGNQI
                     GLATGLVDEIYHEGYVAKRLEIGAVVGAVPKKNVRREVPAKGDLVILVGGRTGRDGCG
                     GATGSSKSHDSSSIEKSGAEVQKGNAPEERKLQRLFRNPEVVRLIKRCNDFGAGGVSV
                     AVGEIADSLRINLNKVKKKYEGLDGTELAISESQERMAVVVEEKDAEKFIAFANEENL
                     EATVIAEVTDNNRLVMEWNGRVIVDISRDFLNSNGAEKHTKVKVKTPADWNNIKAATD
                     FTTAIKELAGDLNVCSKKGLSERFDSTIGAGTVLMPFGGKYQMTPPMAMAAKIPVLNG
                     NTNTCSVMSWGYNPFIAEKSPYHSAYLAVVESVAKLISAGAPLDDCYLTFQEYFPRPG
                     SDPERWGMPLAALLGALKAQTDLKIASVGGKDSMSGTFENLDVPPTLVSFAISVAKTD
                     NIISPEFKKAGNKVYLISPEYSKNGLPVAESLKAVFQRVHKLISSKTVISASAPTYGG
                     ILADIMKMCFGNRLGFRFSDTIDLKQLISYAYGSIIVESPEKLTDAILLGEVTGEYTL
                     RFKDESVSLTEIQEIYENKLESVFPTKPKTETSEKPQRFEFKNDKLFAAKTKVSRPKM
                     LIPVFPGTNCEYDTMRAAEKAGLETEIFIIKNLNSADVAESITEFAKRIESSQIIFIP
                     GGFSGGDEPDGSGKFITAFFRNPLITEKVRELLKIRDGLMCGICNGFQALIKLGLVPF
                     GDILEADASFPTLTFNTIGRHQSMIVRTVIASNNSPWLMNTNVGNIYSVPISHGEGRF
                     ICSDELMSELAANGQIATQYVDLDSEPTYDIRFNPNGSFHAVEGILSPDGRVFGKMGH
                     SERIGDGLYKNVPGNYDIGLFDSAYKYYNI"
     gene            6700..8208
                     /locus_tag="PLD98_06065"
     CDS             6700..8208
                     /locus_tag="PLD98_06065"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008420116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQA05848.1"
                     /translation="MAFYYKEPSHTFSEYLLIPGYSSEECIPSNVSLRTPVVKYKKGE
                     EPALSMNIPLVSAIMQSVSGEKLAIALAKEGGISFIYCSQPIEEQAQMVARVKAHKAG
                     FVVSDSNITPDATLADILALKEKTGHSTVAVTHDGTCNGKLLGLVTSRDYRVSRMDLS
                     TKVHTFMTPFESLVYADKNTTLKAANDIIWNHKLNVLPIVDDDQRLLYLVFRKDYDFH
                     KENPHELLDSKKRYVVGAGINTRDYKERVPALIEAGADVLCIDSSEGFSAWQKNTLSW
                     IRENYGDTVKVGAGNVIEKNGFYFLAEAGADFIKVGIGGGSICITREQKGIGRGQATA
                     LIDVVEARDEYFERTGIYIPLCSDGGIVHDYHITLALAMGADFVMLGRYFARFDESPT
                     NKVSIGGTYYKEYWGEGSNRARNWQRYDHGGAPILSFEEGVDSYVPYAGSLKDNVTMT
                     LNKVKATMCNCGALSIPELHEKAILTLVSSTSIVEGGAHDVILKDNIDFNRK"
     gene            8238..8936
                     /locus_tag="PLD98_06070"
     CDS             8238..8936
                     /locus_tag="PLD98_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HQA05849.1"
                     /translation="MKPFLMEMHLHTSESSNCGRVSAADAVRLYKEAGYDGIVVTDHY
                     SSGTFKRKIPRDIPYKDQVEIYLKGYRAALKAAGDDFTVLLGMELSLNENDNDYLVYG
                     VTEEFLIKAGYLLDLSLEEFVELAHANGCLVYQAHPFRKYMTVTKPELLDGIEVYNGC
                     PGSNSNNEFSMMWANKYGLRGVSGSDFHQLKDLAYGGISFPEPIKTNEDLVRALKGKY
                     SLRVTIDPKYNCVI"
     gene            complement(8988..>9397)
                     /locus_tag="PLD98_06075"
     CDS             complement(8988..>9397)
                     /locus_tag="PLD98_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05850.1"
                     /translation="WGISNMLRAFTSPLFILFTNPLISVVPRILVGLFAGLVSSALLK
                     KTKLPLTVVGVITAIVGTLTNTVLVLSALHIFGDMLESYKEMYKIFQSIISTIISVNA
                     TIEIVAAIIIVPAVGVAIFKAMKNYGIEIKRKA"
BASE COUNT         2720 a         2189 c         2244 g         2244 t
ORIGIN      
        1 taatcgttac caacggcgac cagaccgata caatttacga ctatctctca aacggccgaa
       61 cctttgaaga agcccttaat atcaggagct ttgagccaga tactcccaac tataccccca
      121 gaatcagcgg aataatgcat attaacgaag gcgcagaatt taagctcagc attttaaaaa
      181 gcgccgacgg aaacgctgcc tcctgcaata gatttgtgta ctcgtatgac gggggacttg
      241 gcggcggccg ctttattcat acatacatgg gcgactccac acctttaagg agctttgagg
      301 gtgagccctg gtttgttgag ctttctaatg atattgacga aacaaccaag gatatttgga
      361 gctcgctgaa cgaagagaac aaaatatccc tgtttgtaag atacatcggc atttatacag
      421 gctttagcga aacaagaatt gtgaataaac acttaaaata aagggcgggt ttgtatggac
      481 tttatagagc ttaaatacgg ctgcaatcct catcaaaagc cgtcaaagat ttatgtggac
      541 agcggaaaac ttccgcttga agtgcttaac ggcaccccag gatacataaa cttcctagac
      601 gcacttaacg gctatcagct tgtaaaggag cttaaagaag caacgggact tcccgctgcg
      661 gcgtcattta agcatgtaag ccccgcgggc gccgctgtgg gtctccccct tgacgacacc
      721 ttaaagaaga tgtatttctg cgaggatttg gagggcttaa acgattctcc catagcctgc
      781 gcgtatgcaa gggcaagggg caccgacaga atgtcctcct tcggcgattt tgtcgcgttg
      841 agcgatacct gcgaccttac caccgcaaaa ctcctaaaaa gcgaggtttc cgacggcata
      901 atcgcacccg actacacaga cgaagctctt gaaatcctaa aggcgaaaaa gcgcggaacc
      961 tatgcgatta ttaagattga cccgaattat gttcccgagg agcttgaggt aaggcaggtt
     1021 tacggcatca aattcctgca gggcagaaac aattctgtta ttgataaatc acttcttgaa
     1081 aacatcgtca ccaagaataa aaacataccc gacagcgcca aaaccgattt gctgattgct
     1141 cttataaccc ttaaatacac ccagtcaaat tcggtttgct atgcattcgg aggtcagacc
     1201 atcggcgtcg gcgcaggtca gcagtcaaga atccactgca cccgcctggc tggcgataag
     1261 gctgatgtat ggcagctgag aaggcatgaa aaggttcaga atcttaaatt cctgcccacc
     1321 cttaaaagag cagacaggga taatctaatc gatatttata tcaccgaagg actttcttca
     1381 atccttaaaa acagcgactg gagaaactac tttgccgaat gccccgagga gctgacatct
     1441 gaagaaaagc gcagctacct tgactcatta aagggcgtat ccctcggcag cgacgccttc
     1501 ttccccttct cagacaatat agagagggca taccgcagcg gcgtatcctt cgttgctcag
     1561 ccgggaggct ccgtcaatga cagcgcagtc attgaagcct gcgacaaata cggcatggta
     1621 atggcgttta ctggccttag actcttccat cattaatacg gcgtgtgagg taaatatgaa
     1681 catacttgta gtaggctctg gcggcagaga gcatgcgatt ataaagaaac tgaaagaagg
     1741 cagacccgac ttaaatatct ttgctgtccc aggcaacggc ggcatcgcaa gcgacgctga
     1801 atgcgtcccc gaaatatccg ctaaggatat tgacaaaata gccgactttg ccgaaaagaa
     1861 caaaattgac tttgcggttg tggctctcga cgacccgctg gttttgggag ctgtggacaa
     1921 gctgcatgaa aagggtattc cctgctttgg accgaataaa aaagccgcaa ggattgaggg
     1981 cagcaaagtt tttgccaaaa atcttatgca gaagtacggc atccccaccg ccaagtattc
     2041 ggtgtttgaa agcgctgacg aagctcttgg gttccttaat gcaaccgagt tccctgttgt
     2101 tgttaaggcg gacggcttag ctctcggcaa aggcgtattt atctgtcccg acagagatac
     2161 agccgaaaag gcaattaaag agataatgct aaataagatt ttcggcgaaa gcggcaacaa
     2221 aatcgtaata gaggagtacc ttacaggtcc cgaggtgtcg gtgctttgct ttaccgacgg
     2281 caaaaccatt atccccatgg tttcttccat ggaccacaag agagctcttg acggtgataa
     2341 gggatttaat acgggcggca tgggcacaat agccccgaat ccttacttta ccgaagaagt
     2401 cgcaaaagaa tgcatggaaa aaatctttat ccccacaata aacgctctta acgcagagga
     2461 cagcaagttt aaaggatgcc tgtatttcgg gcttatgata acaaaggacg gacctaaagt
     2521 tattgagtat aactgcaggt tcggcgaccc cgaaactcag gttgtgctgc cgcttttaaa
     2581 aaccgatttg ctcagcatta tgcttgcgac ggaaaatgaa tgccttgact caatagatgt
     2641 tgagttttat gacaagagcg cctgctgcgt ggtaatagca tctggcggct accccgaaaa
     2701 gtacgaaacg ggctttgaaa tcgaaatcga tgaaaaatcc ttgccggaga atatcagtat
     2761 tttccaggcg ggtacaaaac ttcggaacgg aaagctcgtt acaagctcag gcagggttct
     2821 gggtgttacc gcggttgccg atacattaaa gcaggctatc atcgacgcat ataaagcctg
     2881 ctcgcttatc agctttacaa actgctttta cagaaaagat ataggccaaa aggctcttga
     2941 aattatcaat aaaaaataaa gtcggtcagg agatataaat ggtttacaga gtttatgttg
     3001 agaaaaagaa aggccttgcc catgaggcgg cggccctctt gaatgacatc agaaccaatc
     3061 tcggcgttac ttcgcttaaa aatatcagaa tcttcaacag atatgatgtt gagaatattt
     3121 ctaaggagct ttttgacgac gcaatttccg ccgtgttctc agaacccatg gtggatgatg
     3181 tgtattacga gctcccgaat cttgaagacg cttatatttt tgcggtagag ttcctgcccg
     3241 gtcagtatga ccagagggct gactccgccg ctcagtgcat tcagcttatt tctcaaaagg
     3301 accgtccgct tgtaaggaca gcaaaggttt acgctctttt cggagagttg accgaaagcg
     3361 aaatcggcgc aataaaaaaa caccttataa accctgttga aagcagggag gcagctctcg
     3421 aattacccca aacattaaac ctcgactacg atatcccaca aaccgttgaa accctggacg
     3481 gctttattga tttaaaggac gaggatataa aggaatttgt aaaaaaatac gcccttgcga
     3541 tggatgagga cgaccttaaa ttctgcattg aatactttaa gtccgaaggc agaaatccca
     3601 caattactga gcttagggtt cttgatacat attggtccga ccattgcagg cacaccacat
     3661 tcctgactac cattgacagt gtagaaattg acgacgccga aataaaatcg gcttatgatt
     3721 tatatttgac tctgagagcc gaaacgggca gagcggataa gaaaatcaat cttatggata
     3781 tcgccaccat cggcgcaaga tatcttaaaa agcagggact gcttgaaaag ctcgatgagt
     3841 ctgatgaaat caacgcctgc acggtcaaaa tcgatgttga catagacggt gaaaatgaag
     3901 aatggcttct tcttttcaag aatgaaaccc acaaccaccc gaccgagata gagccctttg
     3961 gcggcgccgc cacatgcata ggcggagcaa taagggaccc gctgtcgggc agggcatatg
     4021 tatatcaggc gatgagagtt accggcgcgg gcaacccttt aaaaccgctg tcagaaacca
     4081 ttgaaggaaa gctgccccag cgcaagattg ttacaaccgc cgccgacggc tacagctcct
     4141 acggcaatca gattggcctt gcgacgggct tggttgatga aatataccat gaaggctatg
     4201 tagccaaacg gcttgaaatc ggcgctgtag taggcgctgt gcctaagaaa aatgtaaggc
     4261 gcgaggtgcc cgcaaaaggc gatttggtta tccttgtggg cggcaggacc ggcagagacg
     4321 gctgcggcgg agcgacaggc tcttcaaagt cccatgactc atcatcaatc gaaaagagcg
     4381 gagcagaggt tcaaaagggc aacgcccccg aggagagaaa gctccaaagg cttttcagaa
     4441 atcccgaggt tgtgcgcctt attaagcgct gcaatgactt cggcgcaggc ggcgtttcag
     4501 ttgccgtcgg agagattgcg gacagcctta gaataaatct caacaaggtt aaaaagaagt
     4561 acgaaggtct tgacggcact gagcttgcca taagcgaatc tcaggaaaga atggccgttg
     4621 tcgttgagga aaaggacgct gagaagttta tagccttcgc caatgaggaa aaccttgagg
     4681 caacggttat cgctgaggtc actgacaaca accgccttgt catggagtgg aacggcaggg
     4741 taattgttga tatatcccgc gatttcctta actccaacgg cgcggaaaag catactaaag
     4801 taaaggtcaa aaccccggca gattggaata atattaaagc cgccacagat ttcactactg
     4861 caataaaaga actggcgggc gaccttaatg tttgctcaaa gaagggctta tcggagcgtt
     4921 ttgactccac catcggcgcg ggaacggtgc ttatgccttt cggcggaaaa tatcagatga
     4981 ctcctccaat ggcaatggca gctaaaattc ctgtgctaaa cggcaatact aatacatgtt
     5041 cggtaatgtc gtggggctat aatcccttta tagcggagaa atcgccctac cacagcgcat
     5101 atcttgcagt tgttgagtct gtagcaaagc ttatttccgc cggcgccccg cttgatgact
     5161 gctatcttac cttccaggag tatttccccc gcccgggcag cgaccccgaa agatggggca
     5221 tgccccttgc cgctttgctc ggagctttaa aggctcagac tgaccttaag attgcatcag
     5281 tcggcggcaa agactcaatg agcggtacat tcgaaaacct tgatgttccg cccacactcg
     5341 tctcattcgc tataagcgtg gcaaagacgg acaacattat ttctcccgag tttaaaaagg
     5401 caggaaataa agtatattta atctctcccg aatactccaa aaacggcttg cctgtggctg
     5461 aaagccttaa agcggtattc cagagagttc acaagctcat ttcctcaaaa accgtaattt
     5521 ccgcatctgc tcccacatat ggcggcattt tggcggacat aatgaaaatg tgctttggta
     5581 acagactggg ctttagattc tctgatacaa tagaccttaa gcagcttatc tcatacgctt
     5641 acggctctat tattgttgaa agccctgaaa aactgacaga cgcgattctt ctgggtgaag
     5701 taacagggga gtacacatta agatttaaag acgagagtgt atcccttacc gaaattcagg
     5761 aaatctacga gaataaactt gaaagcgtat tccccacaaa acccaaaacc gagacaagcg
     5821 aaaagccgca gagatttgag tttaagaatg ataaactctt tgcggcaaag accaaagttt
     5881 caagacctaa aatgctgata cccgttttcc ctggcaccaa ctgcgaatat gacacaatga
     5941 gggctgcgga aaaagcgggt ctcgagactg aaatatttat aatcaaaaac cttaacagcg
     6001 cggatgttgc ggagtccatt acagagtttg caaagagaat tgaatcctct cagattatat
     6061 ttatccccgg cggattctcg ggcggcgacg agcccgacgg ctcaggcaaa ttcatcaccg
     6121 cattcttcag aaatccgctt attaccgaaa aggtaaggga gcttttaaag attcgcgatg
     6181 gtcttatgtg cggcatatgc aacggcttcc aggcgcttat aaagctcggt cttgtgccct
     6241 ttggcgatat tcttgaggcc gacgcctcat tcccgaccct gacatttaat acaatcggca
     6301 gacatcagtc aatgatagta agaaccgtca tcgcctccaa caactcgcct tggcttatga
     6361 ataccaatgt aggcaacatt tacagcgttc ctatctccca cggcgagggc aggtttatct
     6421 gctccgacga gctgatgagc gagcttgccg caaatggtca gattgctacc cagtatgttg
     6481 accttgacag tgagcctacc tatgacataa ggtttaatcc caacggctca ttccatgccg
     6541 tagagggtat actttcacct gacggcaggg tattcggcaa aatgggtcac tctgagcgca
     6601 taggcgacgg tctttataaa aatgtacccg gcaactatga tataggatta ttcgactcag
     6661 cttacaaata ctacaatatc taaagcaaag gagagaaata tggcttttta ttacaaggaa
     6721 ccctcacata ccttcagcga atatcttttg attccaggct actcctcgga agaatgcatt
     6781 ccttcaaatg taagtctcag gacacctgtc gtaaagtata aaaagggcga ggagcccgcg
     6841 ctttcaatga atatcccctt ggtatcggca attatgcagt ccgtatcagg cgaaaaactc
     6901 gccattgccc ttgccaaaga gggcggaatt tcattcatct actgctctca gcccatagag
     6961 gagcaggctc agatggtggc aagggttaag gctcacaagg cgggctttgt tgtcagcgac
     7021 tccaatatca cccccgacgc cacccttgcg gatattctcg ctttgaagga gaaaacaggt
     7081 cattccaccg ttgccgttac ccatgacggt acatgcaacg gaaagctcct gggtcttgtt
     7141 acaagcagag actacagagt gtcaagaatg gatttgtcca ctaaggttca cacctttatg
     7201 actccctttg agtccttggt atatgccgac aagaacacca cattaaaggc tgccaacgat
     7261 ataatctgga accataagct gaatgtcctg cccattgtgg atgacgacca gcgcctttta
     7321 tatcttgtat tcagaaagga ctacgacttc cataaggaga acccccacga acttcttgac
     7381 tcaaagaaaa ggtatgtggt gggcgcaggc atcaatacaa gggactacaa ggaaagggtt
     7441 cccgctctta ttgaggctgg cgcggatgtt ctgtgcattg actcctccga gggcttcagc
     7501 gcatggcaga agaacacact ttcatggatt agggagaatt acggcgacac tgtaaaagta
     7561 ggagcaggca atgtaattga aaaaaacggc ttttacttcc ttgccgaggc tggcgcggac
     7621 tttattaagg tcggcatagg cggcggttcc atctgcatta cgagagagca gaagggcata
     7681 ggcagaggac aagctactgc tcttatcgat gttgtcgagg caagggacga atactttgaa
     7741 agaacgggca tttatatccc cctttgctcc gacggcggca ttgttcacga ctaccatatt
     7801 acccttgccc ttgcaatggg cgccgacttt gtaatgctgg gcagatattt cgcaagattt
     7861 gacgagagcc cgaccaataa ggtaagcata ggcggcacat attataagga gtactggggc
     7921 gaaggctcca acagagcaag gaactggcag cgctacgacc atggcggagc gcccatactt
     7981 tccttcgagg agggcgttga ctcctatgtg ccgtacgcag gttctctaaa agacaatgtt
     8041 accatgactt taaacaaggt taaggcaaca atgtgcaact gcggagctct ttcaatccct
     8101 gagctgcacg aaaaggctat ccttaccctt gtttcatcta caagcattgt tgagggcgga
     8161 gcccacgatg ttattctcaa ggacaatatc gactttaacc gcaaataatt ttctaatcta
     8221 cgagaggtct tgatattttg aaaccattct taatggaaat gcaccttcac acaagcgaat
     8281 ccagcaactg cgggagggtt tccgccgcag acgctgttcg cttatacaaa gaggccggct
     8341 atgacggaat tgtcgttacc gaccactaca gctcaggcac attcaaaaga aagattccgc
     8401 gggatatacc ctacaaagac caagtagaga tttacctgaa aggctacagg gcggctttaa
     8461 aggctgcggg cgatgatttc accgtgcttc tcggcatgga gctttctctt aacgaaaatg
     8521 acaatgacta tcttgtttac ggggtaacgg aggaattcct tattaaagcc ggctatctcc
     8581 ttgatttatc ccttgaggag tttgttgaac tggctcacgc aaacggctgc cttgtatatc
     8641 aggctcatcc cttcagaaag tatatgaccg tcacaaaacc cgaacttctt gacggaatcg
     8701 aggtttacaa cggctgcccg ggaagcaatt caaacaatga attctccatg atgtgggcaa
     8761 acaagtatgg tttaagggga gtttcgggct ccgatttcca ccagcttaaa gaccttgcat
     8821 acgggggaat cagcttcccg gagcctatca agacaaacga ggacttggta agagctttaa
     8881 agggaaagta ctccctgcgc gtaaccattg acccgaagta caattgcgta atctaaaaaa
     8941 gaatcctccg actgaatgtc ggaggatttt aatttccttg gaaattgtta tgcttttctc
     9001 ttaatctcaa tgccgtagtt tttcattgcc ttgaagatag caacacccac ggcgggaaca
     9061 atgatgattg cggctactat ctcaattgtg gcatttacgc tgattatggt tgagataatt
     9121 gactggaata ttttatacat ctctttgtag ctctcaagca tatcgccgaa gatgtgaagc
     9181 gcggaaagaa caagcacggt gtttgtaagc gtgcccacaa tagcggtaat tacgccgaca
     9241 actgtcaagg gcagctttgt tttctttaaa agcgcgcttg agacaagacc tgcgaataga
     9301 cctacaagaa ttctaggaac gaccgaaatc agcgggtttg taaagagtat gaacaggggg
     9361 cttgtaaaag ctctgagcat attggaaatg ccccata
//
LOCUS       DAONVQ010000081         9238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5731_length_9238_cov_4.310465, whole genome shotgun sequence.
ACCESSION   DAONVQ010000081 DAONVQ010000000
VERSION     DAONVQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5731_length_9238_cov_4.310465"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLD98_06080"
     CDS             complement(<1..582)
                     /locus_tag="PLD98_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05851.1"
                     /translation="MIDKELKLRGVPDILRGTDGERITDKKTWEENREKLKELLCLHE
                     YGFLPPEPKDLKFEILRSDERFFANTAIYQKILITASLENGEFSFPVQFVRPKKEGRH
                     KTIVFLNFRDGVPDKYYPAEEICDRGFAVATIYYKDVTGDDGDFTNGLAGVLFGGKER
                     NEHDPGKIMMWAWAAMRAADYLQTLDSADLESLA"
     gene            771..1181
                     /locus_tag="PLD98_06085"
     CDS             771..1181
                     /locus_tag="PLD98_06085"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HQA05852.1"
                     /translation="MRCEYCGSEVQGNFCGNCGAPVPQESRIENDSYKSNPYSNSWDT
                     DYNKFSNDSGSTYYNNYSYTTTYSVASNKSKIVALVLCFLFGIFGIHRFYVGKVGTGL
                     IWLFTGAIGGLGWIVDLILILTGNFKDSAGLPLK"
     gene            complement(1247..1654)
                     /locus_tag="PLD98_06090"
     CDS             complement(1247..1654)
                     /locus_tag="PLD98_06090"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HQA05853.1"
                     /translation="MTDELQKAVSKLHDSGCAIVVANGEKVIASAETSLKPLIEIVKT
                     SPEILRGAAVADKIVGKAAALLLVHSGVREVYAEILSDFAVPVLERAGIQYHYNKKVP
                     FIKNRAGDDTCPMEKKVLYIDDPETAVSIFLNL"
     gene            complement(1686..2255)
                     /locus_tag="PLD98_06095"
     CDS             complement(1686..2255)
                     /locus_tag="PLD98_06095"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HQA05854.1"
                     /translation="MSLSRKTALGGLFIALSVLLPQVIHLIGAADLGRMLLPMHIPVL
                     LSGFVLGPVFGLTVGAISPILSCLLTNMPPVSRLAFMVIELAGYGFMSGLLFRTLKLY
                     KTRYGTALSLAGAMIFGRLLYALSLFVASDLLGISQIGPIAAREAVTAGLIGIVIQII
                     LVPTIIYLLRKIRVIDPADTQKKRNQTAG"
     gene            complement(2268..3410)
                     /locus_tag="PLD98_06100"
     CDS             complement(2268..3410)
                     /locus_tag="PLD98_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQA05855.1"
                     /translation="MDYRIVGKTNVSASLLGYGCMRFPTTADGEIDEVKAEKLLDTAY
                     ENGVNYFDTAYYYHSGKSEPFVGKVLSKYDRGSYYIADKLPHWPVKTLDNAKRIFNKQ
                     LERLQTDYIDFYLLHSLDKAAWKRLSELGVPEYCESLKAEGKIKYFGFSFHDDYEVFE
                     EILTSRAWDFCQIQLNYMDTEHQAGMKGYELAEKMGIPVIIMEPLKGGKLATLPEDLT
                     EELKKHNPDASVASWGFRWVAGLPNVKVVLSGMTTMEQLEDNLKTFSGFKPLTEEEQQ
                     IVASLPEKIKRKVNNECTNCKYCMPCPSGVDIPKNFRVWNEYGMFRNKQAIAWQWENE
                     IDEDEKASNCVACGQCEEACPQHIRIIEDLSRLQKELDSVVESLKR"
     gene            complement(3571..3647)
                     /locus_tag="PLD98_06105"
     tRNA            complement(3571..3647)
                     /locus_tag="PLD98_06105"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3611..3613),aa:Pro,seq:tgg)
     gene            complement(3793..5991)
                     /locus_tag="PLD98_06110"
     CDS             complement(3793..5991)
                     /locus_tag="PLD98_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQA05856.1"
                     /translation="MAVNKITSFIMSVLLFFSSILLFPWFCTPAPVPAVLSEYTEKPT
                     ETVVGDFYVSPNGSDEGDGSLSSPFKTIERAIEAVRSLDKTGKDSVTVCIMAGEYRTN
                     GLVFTAADGGTESCPINYCAFGDGEAVINGGFSLTADDFSPVSGEAAERLKSSVRDKV
                     LKANLFELGLTAEEIGEMKAIGTYNTAHLYGKGSGVYCELFFADRRMTLARYPNSGEF
                     LKTGDVVYNSLEENNAPQGWSGYTNPSGDKFKMDENTASRAASWSTLDDVWMMGYWRY
                     DWADGSTPVKSVSGGTIETAYASNYGIRKDAPYYFFNVLEELDSPGEYYIDREAGILY
                     FYPDKDLSQGEIFLTITQNPVIKSNADYLIFENLTIQGTRGDSVSITANNNIVKGCTV
                     KNIGGTAVVANGCNNLITECEVYHTGRGGIYLYGGDRERLIPGNNKADNNYIHDWSEI
                     YLTYQPGVRLNGVGNICSHNEMCRSPHEAITYSGNNHIIEYNEIYEVVLRSEDAGAIY
                     AGRSWTDYGTVIRYNCIYNLGSGGYSPNGIYLDDGLSGQSVYGNLLINVPGYGLMLGG
                     GRDLSVKNNIVIGSQKGIYYDARVIEGVLDENFWFKHGRKGQVLWTGLYNSPWQTDIW
                     KEAFPQMSKISDDFDDAENPYFGPNPAFSVVSDNIVISKSPVPSIGTISPFVTKYSAV
                     EHNSVINFGAIHSVFPGIAKGDYTMNANSAFLYQLSDDFEILPLSEFGRY"
     gene            6172..6612
                     /locus_tag="PLD98_06115"
     CDS             6172..6612
                     /locus_tag="PLD98_06115"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQA05857.1"
                     /translation="MLLSIALSSDMILRIFLLICFLCPLLFAFTLMLWERSGSPVIAT
                     LTDKATGKVYEISSWETSIGRAKLCDVVLSYATVSRLHAVITRRKKSWIIFDTNSSLG
                     IKVNGLIVKNKASLYDGDTISLGGAEFIFRALELPERKKKTRKK"
     gene            6654..8126
                     /locus_tag="PLD98_06120"
     CDS             6654..8126
                     /locus_tag="PLD98_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQA05858.1"
                     /translation="MKYGVLSKIADSRFLWLLIYVPALAFEVIYAGKVISNAGDAAAS
                     LTTYFTLFLLIQTGCFVFFKLILGKRFELEIAAFMLSFLSIAITASVYPKRTLVQFLT
                     FALGVVLYYTMLWILQNTRWITSLRVPAALGAVGLLAATIVFGTTYRGARNWIFIGDF
                     SIQPSELAKILFIFVGAVTLDKLQSIRSLSKYILFAACCIGIIFVMKDFGTALIFFAT
                     FLIISFMRSGDIKKILLICAAAAVGAVAIVIFKPYVANRFMTYRHIWEYMDSGGYQQT
                     RVLIYSASGGLFGVGLGNGQLRNVFAATEDLVFGVLCEEFGIILAFTVALTYVAFAFY
                     SIKMAKTARSTFYSIAACAAAGLMLFQAGLNIFGVTDLLPLTGVTLPFVSRGGSSMLS
                     SWGLLAYLKAVDPLTYPVKKLSPRPAPVPTAAKSEYADYLGDGYDDVFIERKKAPRTG
                     IYISAAKKERQAARPAQSRQTAGKSASGKGRNTNKGRRTK"
     gene            8123..>9238
                     /locus_tag="PLD98_06125"
     CDS             8123..>9238
                     /locus_tag="PLD98_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQA05859.1"
                     /translation="MRITINRSKMLYLLVIAFMGGLTILLVSLFANGRTYAMHTANRH
                     LYGSSAVESFGLIEDRNGVMLADTKNKKRVYSSDQGIRRALLQTIGDTSFYISSGVHN
                     TFKSELTGFNMIWGINSAASGSKSNLKLTLDAKLCKTALNALEGQKGTVGVYNYKTGE
                     VIVMVSSPTYDISNKPSDINTDKSGKYDGVYLNKLLSGVYVPGSVFKTVTAAAAIENI
                     PDVMTRKFNCAGQYKAPDGGVIKCMGTHGTISFEKALNVSCNSAFAQLAIEIGKEKLQ
                     KTAEKMGLTQSFVCDRVNTAKGNVDLSEAGPASLGWAGIGQHTILVSPVAVMRMMGAI
                     ANGGKAVNPYFVESVIQNDGTVLYKGGSSSEKLLNVTT"
BASE COUNT         2257 a         2259 c         2247 g         2475 t
ORIGIN      
        1 cgctaaactc tcaaggtctg ctgagtcaag agtctggaga tagtccgcgg cgcgcatggc
       61 agcccatgcc cacatcatga ttttgccggg gtcgtgctcg ttcctctcct tgccgccgaa
      121 cagcacaccc gcaagaccgt ttgtaaaatc accgtcgtcg ccggttacat ccttatagta
      181 aattgtggcg acggcaaagc ccctgtcgca aatctcctcg gcgggatagt atttgtcggg
      241 aacgccgtct ctgaagttta aaaagactat ggttttgtgc ctgccctctt ttttggggcg
      301 gacaaactga acgggaaatg aaaattcccc gttttcaagg ctggcggtta tcagaatttt
      361 ttgataaata gcggtgttgg cgaaaaaacg ctcgtcgctc cggagaattt caaattttaa
      421 gtccttcggt tcagggggca aaaagccgta ttcatgaagg cacaaaagct ccttcagctt
      481 ctctcggttt tcctcccaag tttttttgtc ggttattcgc tccccgtcgg tgcctcttag
      541 aatatcgggc actccccgta atttcagctc cttatcaatc attttcctca cccttcggct
      601 ttttgtttta tcttatcaga tttcatatta ataagaaaag agctcataaa aacaaaaaac
      661 aactttgctt gaagtcggtg cgtttaagct atataataaa aaggaaaatg acgggtattg
      721 gagattctcc gctcggaaaa agctttgtaa atttcaaggg gtgttatttt gtgagatgtg
      781 aatactgcgg gtctgaggtt cagggaaatt tctgcggcaa ctgcggagct cccgtaccac
      841 aagaaagccg tattgagaac gacagctaca agtcaaatcc atactctaac tcatgggata
      901 ccgattacaa caagttcagc aacgattcgg gcagtactta ctacaataat tattcataca
      961 caaccacata ctcagtcgcg tcaaacaaga gcaaaatagt ggcacttgtt ttgtgctttc
     1021 ttttcggcat tttcggtatt cacaggtttt atgttggcaa ggtcggcacg ggacttattt
     1081 ggctctttac cggcgcgatt ggaggtctcg gctggattgt ggatttgatt ctcattttaa
     1141 ccggcaattt taaggacagc gcaggtttgc ctctaaaata aaagccgtat aaagttttag
     1201 cggaaaaaac acagggtgga gaaatccacc ctgctgtatt ttgatattat aaattcaaaa
     1261 atatgctcac tgcggtctcg gggtcgtcaa tatagagaac tttcttttcc atagggcagg
     1321 tgtcgtcgcc tgccctgttt tttataaagg gcaccttttt attgtaatga tactgtatgc
     1381 ccgcgcgctc aagaacggga acggcgaagt cgcttaaaat ctccgcgtac acttccctga
     1441 cgcccgaatg cacaaggagg agggcggctg ccttgccgac tattttatcg gcgaccgccg
     1501 cgccgcggag aatttcgggg cttgttttaa ctatttctat aagcggttta agactggtct
     1561 cagcggaggc aattaccttt tcgccgtttg cgacgacaat agcgcagccg ctgtcgtgaa
     1621 gtttacttac cgccttttgg agttcatctg tcatatgtaa atcttctttc tttaagcttt
     1681 gggtgtcagc ccgctgtttg gtttctcttt ttctgcgtat ctgcggggtc aattacccgt
     1741 atctttcgca gcaggtagat tatagtcggc acgaggataa tctgaattac tattccgatt
     1801 aatcccgcag tgaccgcttc ccgcgccgcg atgggtccaa tttgagaaat ccccagaagg
     1861 tctgaggcga caaatagaga aagagcgtac aaaagccttc cgaatatcat ggcgcccgca
     1921 agggaaaggg ctgtgccgta acgggtttta tagagcttaa gggttctgaa taaaagtccg
     1981 ctcataaagc cgtagcccgc cagctcaatg accataaaag ccagccttga cacgggcggc
     2041 atattggtga gcaaacagct caatatgggg gaaatcgctc ccaccgtaag cccgaaaacg
     2101 ggacccagca caaagccgct gagcagaacg ggtatgtgca tgggcagcag cattctgccc
     2161 aaatcagccg ctccgataag gtggataacc tgcgggagca gtacggacaa agctatgaac
     2221 aagcctccca gagctgtttt tctgcttaaa ctcatcttct ctccccttta acgctttagg
     2281 gattctacca cgctgtccag ttccttctgc aggcggctta aatcctcaat aatgcggata
     2341 tgctgagggc atgcctcctc gcactgaccg caggcaacgc agttggaagc cttttcgtcc
     2401 tcgtcaatct cgttttccca ctgccacgct atggcctgct tgttcctgaa cataccgtac
     2461 tcattccaga cgcggaagtt cttaggaatg tccacgcccg aaggacaggg catgcagtat
     2521 ttgcagttgg tgcactcgtt gttgaccttg cgctttatct tctcgggcag cgaggcaaca
     2581 atctgctgtt cttcctcagt cagaggctta aagccgctga aggttttaag gttgtcctcc
     2641 agctgttcca ttgtagtcat gccgctgaga accaccttaa cattgggcag acccgctacc
     2701 cacctaaagc cccatgaggc tacggaggcg tcgggattgt gctttttaag ctcttcggtt
     2761 aaatcctcgg gcaaggttgc cagcttgccg ccctttaaag gctccattat tataacgggg
     2821 attcccattt tttccgcaag ctcatagccc ttcattcctg cctgatgctc tgtgtccatg
     2881 tagttcagct gaatctggca gaagtcccaa gccctgcttg tcaggatttc ttcaaagacc
     2941 tcgtagtcat catggaaaga aaagccgaag tatttgattt tgccctctgc tttaaggctc
     3001 tcgcaatact caggaacacc aagctctgaa agtcttttcc acgcggcttt gtccaatgaa
     3061 tggagaagat aaaaatctat ataatccgtc tgcagccttt caagctgctt attaaaaatt
     3121 ctctttgcgt tgtcaagggt tttaacaggc cagtgaggga gcttgtcggc aatatagtag
     3181 ctgcctcggt catatttgga cagcaccttt cccacaaagg gctcgctttt gcctgagtga
     3241 tagtagtacg ctgtatcaaa atagttaact ccgttttcat atgcggtatc aaggagcttc
     3301 tccgccttaa cttcgtctat ttcaccgtcc gcggttgtag ggaatctcat gcatccgtag
     3361 ccgagaagag aagcagacac attggttttt ccgactattc tgtaatccaa atttagccct
     3421 ccttatgtaa gccctattgt ctaatgtaaa ttataatata aggcattaaa atataaaatc
     3481 cccaaaataa taagcccgat gtcgattttt atttctcttg caaaaaaatt gaaaaacccg
     3541 ctcgaacgcg ggtttttctt tttgcacttt tggtcgggat gacaggattc gaacctgcgg
     3601 catcttggtc ccaaaccaag cgctctacca agctgagcca catcccgttt ttcgccttaa
     3661 atttgctatg acattataca caaagacaag gcggtttgtc aaccacagcg cggctgccaa
     3721 agggaggaaa gacagcgggg cgcggctcaa aagcaaaaaa atgaggctgt gtttttcaca
     3781 gcctctttaa gcttaatatc ttccgaattc gcttagcggc aagatttcaa aatcgtcaga
     3841 aagctggtac aggaaagcgg aatttgcgtt catcgtgtag tcgccctttg ctatgccggg
     3901 gaataccgaa tggatggcgc cgaaatttat aaccgagtta tgctcaacgg cactgtattt
     3961 tgttacaaag gggcttatgg tgccgatact cggcacgggg gacttggaga ttactatatt
     4021 gtccgaaact acactgaacg cgggattggg accgaagtaa ggattctccg catcgtcgaa
     4081 gtcatccgag attttgctca tctgcgggaa tgcctctttc cagatgtcgg tctgccacgg
     4141 ggagttataa agccccgtcc ataaaacctg acctttccgt ccgtgtttga accagaaatt
     4201 ctcgtcaaga acaccctcga ttactcgggc gtcgtagtat atgcccttct gggagcctat
     4261 tacgatattg ttcttgacgc tcaggtcacg gccgccgccc agcattaatc cgtagccggg
     4321 gacattgata agcagattgc cgtatacgct ctgacccgaa agtccgtcgt ccaaataaat
     4381 gccgttgggc gagtagccgc cggaacccaa attgtaaatg cagttatatc ttatgaccgt
     4441 tccgtaatcc gtccatgacc tgcctgcgta tatggctccg gcgtcttcgg accggagtac
     4501 aacctcgtat atctcgttat actcgattat gtggttgttg cccgaatatg tgattgcctc
     4561 atggggcgag cggcacatct cgttatggga gcagatattg ccgacgccgt taagccttac
     4621 tcccggctga tatgtaaggt aaatctcgga ccagtcgtgt atgtagttgt tgtccgcttt
     4681 gttgtttccg ggaataagcc tttccctgtc gccgccgtaa aggtatatgc cgccgcggcc
     4741 tgtgtgatag acctcacatt ccgtaatcaa attgttgcag ccgtttgcca caaccgcggt
     4801 gccgcctatg ttctttacag tgcatccctt tacgatattg ttgttggcgg tgattgatac
     4861 gctgtcgcct cgggtgccct gtatggtcag gttctcaaat attagatagt cggcgtttga
     4921 tttgattacg gggttttgtg taatggtaag gaatatttcg ccttggctta aatccttgtc
     4981 gggatagaaa taaaggattc ctgcctcgcg gtcgatgtag tattcgccgg ggctgtcaag
     5041 ctcctcaagc acattgaaga aataataggg agcgtccttt cttatgccgt aattggaggc
     5101 ataagcggtt tcgattgtac ctcccgaaac ggactttacg ggggtggagc cgtccgccca
     5161 gtcatagcgc cagtagccca tcatccagac atcatccaga gtgctccaag aggccgcgcg
     5221 ggaggctgta ttctcatcca ttttgaattt gtcgccggat ggatttgtat agccgctcca
     5281 gccctggggc gcgttgtttt cttcgaggct gttgtagact acatcgccgg ttttaaggaa
     5341 ctctccgcta ttgggatagc gggcaagtgt cattcttctg tcggcaaaga acagctcgca
     5401 gtatactcct gagccctttc cgtaaaggtg agccgtgtta taagtgccta tggctttcat
     5461 ctctccgatt tcctcggcgg taaggcccag ttcgaaaagg ttggccttta agaccttatc
     5521 tcttacagag cttttcagcc tctctgccgc ttcgccgctg acgggagaaa aatcgtccgc
     5581 agtcagacta aaaccgccgt ttatcactgc ttccccgtca ccaaaggcgc agtaatttat
     5641 ggggcagctt tcggttccgc cgtcagcagc ggtgaacaca agaccgtttg ttctgtactc
     5701 gcccgccata atgcagacgg tgactgagtc tttgccagtt ttgtcaaggc tgcgtacggc
     5761 ttcaattgcc ctttcgatag tcttaaaggg acttgaaagt gagccgtctc cctcatcgct
     5821 gccgttcggt gagacataaa agtcgcctac aacagtttca gtcggttttt cggtgtactc
     5881 gctcaaaacc gctggaacgg gagcgggggt gcaaaaccac gggaacaata aaatggagct
     5941 aaaaaataac agtaccgaca ttataaaaga ggttatttta tttacggcca taaattttgc
     6001 acctccgctt tcaacatacc attttcaatg cattatggca ataataacct tgtaaagttt
     6061 tgttgcggaa ttgaaaaaaa cagcgtggtt aggaggccat atagagcgaa cttgacggaa
     6121 agttcttcta gtcgtattat agatttgaat actaattttt agggggttat tatgctgctg
     6181 agcattgctc tttcttcgga tatgattctg aggatattcc tgcttatatg ctttctttgc
     6241 ccccttttgt tcgcctttac cttaatgctt tgggaaagaa gcggcagccc agtaatcgcg
     6301 actttaaccg acaaagcgac gggcaaggtt tatgaaatca gctcgtggga aacatccatc
     6361 ggcagggcga aactctgcga tgtggtgctc tcttacgcca cggtgtcgag gctgcacgcc
     6421 gttatcacaa ggcgaaagaa aagctggatt atctttgaca caaattccag cctcggcatt
     6481 aaggtcaatg ggcttatagt caaaaacaag gcaagtcttt acgacggcga taccatttct
     6541 ctgggcggcg ctgagtttat cttccgcgcg cttgagctgc ctgagagaaa gaaaaagaca
     6601 agaaaaaagt aggcttttgt taggcgtctt aacgccggaa aggtgatgaa ggcttgaaat
     6661 acggagtgct ttccaaaata gccgacagcc gctttttatg gctgcttata tatgtgcccg
     6721 cccttgcctt tgaggtaata tacgcaggca aggttatatc aaatgcgggc gatgcggcag
     6781 cgtcgctgac cacatatttt actttgttcc ttcttatcca gacaggatgc tttgtattct
     6841 tcaaactcat tctgggcaaa aggtttgagc ttgaaatagc cgcctttatg ctctcgtttt
     6901 tgagtatagc gataacggca agcgtttacc ccaaaagaac gctggtgcag tttttgacct
     6961 tcgcgcttgg cgttgttcta tactacacca tgctgtggat tttgcagaac acccgctgga
     7021 ttacaagcct cagagttccc gcggcgctgg gcgctgttgg tctgcttgcc gcgaccatag
     7081 tattcggaac cacctacagg ggagcgagaa actggatatt cataggggat ttttcaatac
     7141 agccctcgga gctcgctaag atactcttta tctttgtggg cgccgtaacc cttgataagc
     7201 tccagtctat ccgctccctt tcaaaatata tactctttgc cgcctgctgc attggaatca
     7261 tctttgttat gaaggacttc ggcacggcgc tgattttctt tgctaccttc cttataatct
     7321 cttttatgcg ctcgggcgat attaagaaaa tactgctcat ttgcgcagcg gcggccgtgg
     7381 gcgccgtggc tattgtgata ttcaagccat atgtggcgaa cagatttatg acctacaggc
     7441 atatttggga gtacatggac agcggcggct atcagcagac aagggtgctg atttactccg
     7501 cgagcggcgg acttttcggc gtgggtctgg gcaacggaca attaagaaat gtctttgccg
     7561 cgactgagga cctggttttc ggcgttttgt gcgaggaatt cggcataatc cttgccttta
     7621 cagtagcgct gacatatgtt gccttcgctt tctacagcat caagatggca aagaccgccc
     7681 gctccacatt ctactccata gccgcctgcg ccgcggcggg gcttatgctc tttcaagcgg
     7741 ggcttaacat cttcggagtc accgaccttt tgccgctgac gggcgttact ctgccttttg
     7801 tcagcagggg cggctcaagt atgctcagct catggggact tctcgcttat ctcaaggcgg
     7861 ttgacccgct gacctatccc gtaaagaaac tcagtccccg tcccgcaccc gtacccaccg
     7921 cggctaagtc cgagtacgcc gactatctgg gcgacggcta tgacgatgtg tttattgaga
     7981 gaaagaaggc tcccagaacg ggaatatata tttccgccgc gaaaaaggaa aggcaggcgg
     8041 caagacccgc ccagagcaga cagacggcgg gaaaaagcgc ctcgggcaag ggtcggaata
     8101 ccaataaagg tcggaggacg aaatgagaat caccataaac aggagcaaaa tgctctatct
     8161 gcttgtaatc gcctttatgg gaggacttac tatcctcctt gtatcccttt tcgcaaacgg
     8221 caggacatac gccatgcata ctgcaaacag gcatctttac ggctcctccg ctgtggagag
     8281 ctttggtctt attgaggaca gaaacggcgt tatgctcgcc gacacaaaga acaaaaagag
     8341 ggtttacagt tccgaccaag gcatccgcag ggctcttttg cagaccatag gagacacaag
     8401 tttctatatt tcttcgggcg ttcataacac tttcaagtcc gagcttacgg gctttaacat
     8461 gatttggggc ataaactccg cggcaagcgg ctcaaagagc aatttaaagc tgacccttga
     8521 cgcaaagctt tgcaaaaccg ctctgaacgc ccttgaagga caaaaaggca cagtgggggt
     8581 ttacaactac aagacgggtg aggttattgt aatggtctcc tcccctacct atgacatctc
     8641 aaacaaaccc tcggacatca ataccgacaa aagcggaaaa tatgacggag tgtatcttaa
     8701 taagctcctt tcgggtgttt atgtacccgg ctcggttttc aagacagtga ccgccgcggc
     8761 ggcgattgag aatatccccg atgttatgac aagaaagttt aactgcgcag gtcagtacaa
     8821 agcccccgac ggcggcgtta taaagtgcat gggcacccac ggcacgataa gctttgagaa
     8881 ggctctcaat gtatcctgca actcagcctt cgctcagctg gcgattgaga taggcaaaga
     8941 aaaactccaa aaaaccgctg aaaagatggg tcttacccag tctttcgtat gcgacagggt
     9001 caatacggct aagggaaatg tggacctttc ggaagcgggt cccgcaagtc tcggctgggc
     9061 gggcatcggt cagcacacca tactcgtcag tcccgtcgct gttatgagga tgatgggcgc
     9121 gattgcaaac ggcggcaagg ctgtaaatcc ctactttgtt gagagcgtaa ttcaaaacga
     9181 cggcacagtt ttatataaag gcggctcaag ttcggaaaaa ctccttaatg tcaccacc
//
LOCUS       DAONVQ010000082         9127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5827_length_9127_cov_4.907959, whole genome shotgun sequence.
ACCESSION   DAONVQ010000082 DAONVQ010000000
VERSION     DAONVQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5827_length_9127_cov_4.907959"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PLD98_06130"
     CDS             complement(<1..954)
                     /locus_tag="PLD98_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQA05860.1"
                     /translation="MNPRIKTLLKYIVPTVLSQCAFFLFLIIDGIFVGNGVGTDALGA
                     VNLALPFVMVVGAVFMLTTIGGVTITAIRIGRKDFDGANDAFMNSLFCTLAVSAVFTL
                     IGVFFTKPLAAMLGAGGVYEKMVTDYLFWYSLFTVPSALAVALQGFGRNDGAPVFVMV
                     STILSTMLNIFLDWLFVFPLRMGLKGAAIATGISQTVGFLIISVHFVMKRGVLRVRRF
                     KLSGLLFKKVLKRGVPELLSQLTGPVMIYCMNLMLMRYIGEAGINAYSIICYIISFAY
                     AVFFGVSEGLQPLFGRSYGDKNEGDLKFYYRAGLILSFGSSF"
     gene            1464..2228
                     /locus_tag="PLD98_06135"
     CDS             1464..2228
                     /locus_tag="PLD98_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQA05861.1"
                     /translation="MKILLIVNDGMRPDCIEGHRLTKCLKENGSYCMDGRTVMPSVTL
                     PCHMSLFHSVDPSRHGIVTNTYMPQVRPIDGLFEVLEHSDKKCAILYNWEPIRDVARP
                     GSIARSEFIHNRFYGLEDTNKYLCERAKEWLKDGDIDFAFLYLGWVDGAGHDFGWCSE
                     GYMYALNTTCDLVYDLVRELGDKYTIIFTADHGGHERTHGTDLDEDMTIPVFFLGEPF
                     EKGKKLEGVSIKDIAPTIASLMGAKIPMEWEGKVVG"
     gene            2263..3426
                     /locus_tag="PLD98_06140"
     CDS             2263..3426
                     /locus_tag="PLD98_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA05862.1"
                     /translation="MLLFTLVYFTSYVTRINYAAIISEIIRSEGILKSTASLALTGSS
                     VAYGIGQILSGYLGDKIQPKTMISAGLLTTIMMNLAVPFLGNPYLTAAVWTVNGLAQA
                     FMWPPLVKIMTHVFTEDDYKKAVVLVSWGSSFGTVAVYLTAPLLITLSGWRAVFVLSA
                     LAALVMLIFWLMKCPKITLALKSQPESKKGGAAPFSFMLFGIIAAIVLQGFLRDGVST
                     WMPSLISETFNLGTNISILTGVGFPLFSVVAFYAVSRIYRARVRNELSLAGIIFFLGA
                     FSALMLSVLRDKNAILTVTLVSLLIGSMHGVNLMLIGMVPRHYEKFGRISLMSGLFNS
                     FTYLGSAASAYGMAAYTERFGWNSMIVFWAVVALTGGIICLSIKKPWLKFTGS"
     gene            3625..4500
                     /locus_tag="PLD98_06145"
     CDS             3625..4500
                     /locus_tag="PLD98_06145"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HQA05863.1"
                     /translation="MIKAAIVGFGNVGRFTFDAIMKAPDMELKFVVSSKDLDIPVPTV
                     KSVDELKDIDVAILCLPSRKVPEVAEKLLSRGISTVDSYDIHSNIAEVRLRLDKIAKA
                     NGAKAIISAGWDPGSDSVVRALMLAMIPRGITYTNFGPGMSMGHTVAVKAIDGVKDAL
                     SMTIPAGSGVHRRMVYVELKDGADFETVAKAIKADDYFVHDETHVIEVDSVEALKDMG
                     HGVNIFRKGVSGSTDNQRVEFNMSINNPALTAQIMVSSARAVLKQQPGCYTMIQIPPI
                     DYLCGDTDELIRALV"
     gene            4549..5394
                     /locus_tag="PLD98_06150"
     CDS             4549..5394
                     /locus_tag="PLD98_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQA05864.1"
                     /translation="MSKKAIAAILVCVILVFSIGGFTGARIIRGRALRIVSAEKSAII
                     KDGETVHYVAHRGFSGAAPENTLPAIEKAGQAGYKYCEFDVYPTKDSVWILMHDEKIS
                     RTTTGSGYIEDYTYEELLKFDIKYGSNIKDYPSLKIPTLDEALDVCEKYGMRPVIEIK
                     GGKAEDLKVLCELIEKKNLKAEPVLISFNFSLLLELRKLTPDTEMWLLVSEIKEDTLS
                     KCREINAGLSFDGKNEKNYPKIKPFVDEGILVAAWTIDDIKLHDTLYSLGVRYITTNR
                     ITPPN"
     gene            5516..7060
                     /locus_tag="PLD98_06155"
     CDS             5516..7060
                     /locus_tag="PLD98_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HQA05865.1"
                     /translation="MLNFLKKLYDKKQNELEKTVNSLNAPLEKLKVGETLEENLQICK
                     RVFADMDVITFKEVACEGEQSLKYLLIYCDGMVDRGIINDNVLKPLMSAPPEDTEDLI
                     GALKKSVIHANDAISTDSFKDIVEFVAAGYTAVFVDGVGEAIALNTKECCGRAVMEPE
                     NEKVIGGPKEGFTEDILKNLALIIKRVRTNELKTKYLTVGRITRTKICLCYFDIIADK
                     NILNQLIQKLNKLDIDGVLDSNYIVELIRDNRYSAFRTVGYTERPDTAIAKMLEGRII
                     VLVDGSPMVLSIPYLFVENFQSSEDYYFNFYYSSFARILRMAAFLLTISIPAFYIAVV
                     AYHHEMLPLPLMTRIAMEQQRVPLPSAIEAVIMIFIFDMLRETGVRMPSSIGQTLSIV
                     GALVIGQAAVEASLVSAPMIIVVAATGITSLLVPKLNAPIIVYRFFFLFLSSVLGFFG
                     FTIAMSIMIIHILNLTSSGVDQVQLDGSLKLHKSKDTFARMPWNFMQTRPDRTTANIT
                     REASDL"
     gene            7060..8193
                     /locus_tag="PLD98_06160"
     CDS             7060..8193
                     /locus_tag="PLD98_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HQA05866.1"
                     /translation="MFKKITALTLSLSFVFLIGCWNYRDLDEMNIVVGIALDYDRMTN
                     QYAMTLEVADLTGSSDESGISGKMIYTKGRTLFEAARNTKRREQDKLFFGSPHVLIID
                     QELAYKQGIEAVMEWFIRDAECRESICVAISRAETASDILTPPKDTKGLVSERLHDII
                     REDGEVNSTTFRAQLYQIYNFLNAPRRCAILPAFHRTQDGERVVPEVLGVAVIKGGFL
                     VGFLSPEETKYLLLADNKMKTTILPISVMGRPTADATLEVLENHSKKSFKYENGQLTL
                     IIKTGTIFSLAENQAQLDIMDKNNVKMIEDATKEEIKYNIESLMNTLQKDLKTDVMGF
                     SDHIYKTKPRLWDEIKDYWDELYENAKVEVEVKVKIVNSAFTK"
     gene            8203..8424
                     /locus_tag="PLD98_06165"
     CDS             8203..8424
                     /locus_tag="PLD98_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05867.1"
                     /translation="MCFLLIVAFFAVVLICDSLTNLKGLSKAGKAAYFVMFAFSFIVL
                     SIFTFNPDFTPFAGIIEKIIDMFVEVKTK"
     gene            8440..>9127
                     /locus_tag="PLD98_06170"
     CDS             8440..>9127
                     /locus_tag="PLD98_06170"
                     /inference="COORDINATES: protein motif:HMM:NF015783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HQA05868.1"
                     /translation="MDNKTLSLNQSICAVLLFNFGSSVIMGISTKVAQDAWISIIIAT
                     VLTIPLFMMYGRILNNFPGKNVYEISETVFGKVGGKIVSAFMVSYCIFLASLVMRNYS
                     EFTQIGTLVETPQEPILILLVITTVYLARSGIKTVGKWSVLMLFVVISVVVLILLVSI
                     NQMELSSMLPIFEHSFSDIFWTAVRIISFPYAETVVFLCLGCCFAANESRTKIFLRSL
                     FFGFFIFMNIS"
BASE COUNT         2349 a         2203 c         2315 g         2260 t
ORIGIN      
        1 aaagctgctg ccgaagctga gtatcagtcc cgccctgtaa tagaacttta aatctccctc
       61 gttcttgtcg ccgtagctcc tgccgaaaag aggttgcaga ccctccgaca cgccgaagaa
      121 caccgcatag gcaaaggaaa tgatgtagca gataatcgaa taggcattta tgcccgcctc
      181 gccgatgtat ctcatcagca taaggttcat gcagtaaatc attacgggtc ccgtaagctg
      241 agagagcagc tccggcacgc cccttttcag taccttttta aacagcaagc cgctgagctt
      301 gaatctcctt accctcaaaa ctccccgctt cataacgaag tggaccgaga tgataaggaa
      361 acctacggtc tgagatatgc ccgtggcgat tgccgctccc ttaagaccca ttctgagcgg
      421 gaacacaaag agccagtcga ggaagatatt aagcatggtg ctgagaatcg ttgagaccat
      481 cacaaagacg ggcgcgccgt cgttgcgccc aaacccctgc aaagctactg ccagcgccga
      541 cggaacggta aagaggctgt accagaaaag gtagtccgtc accattttct catagactcc
      601 gcccgctccc agcatggcgg caaggggctt tgtgaagaaa acgcctataa gggtaaagac
      661 cgccgaaacg gcgagggtgc agaaaagcga gttcataaaa gcgtcgttcg ccccgtcaaa
      721 gtcttttctg ccgattctta tggctgtgat ggtcactccg ccgatggtgg tcagcataaa
      781 gaccgcgccc accaccataa caaagggcag ggcaaggttt acggcgccca gagcgtcggt
      841 gcccacgccg ttgcccacga atatgccgtc tattatgagg aacagaaaga aggcgcactg
      901 gctgagcaca gtgggaacta tatattttaa caatgttttg attcttgggt tcatagattc
      961 tccattttcc atagatgaaa gattttagcg taaacttcaa tattataaac tttttcccga
     1021 gactttgcaa atagacaaaa agtttttgat tttattattt cgcattctaa aaggcgtaag
     1081 cccggcgggt actaacggaa tatcagcgga cggacaagcg ggagagcaaa gcccgtcctg
     1141 ccgagcgtac cgccccgccg ccgcaggggg atgtcacgcc gtagccccgc cgccgagggg
     1201 aaattcctcc ccgccgaggg ggatgttgcg ccgccgcccc gccagaaaca ccgataatat
     1261 cccccatgac acggtttttg acggtctttt acaatccgtc tattgacaaa caaggactca
     1321 aagtactaat gtaattgcga gcggctctca aatcgggctt tctttagtct tttagccttc
     1381 ggggcttaat acattattgg taatagattc atcttttccc cgcccgccgt tttgcggaaa
     1441 actgagaaaa ggagaagtgc tgcatgaaaa tcctgctgat tgtaaacgac ggaatgaggc
     1501 ccgattgtat tgagggccat cggcttacca agtgtctgaa ggagaacggc tcctactgca
     1561 tggacggacg caccgtcatg ccctccgtga cactgccctg ccatatgtcc cttttccaca
     1621 gcgtggaccc ttcaaggcac ggcatcgtca ccaacacata catgcctcag gtaaggccca
     1681 ttgacggtct ttttgaggtg cttgaacaca gtgacaaaaa atgcgccatt ttatataact
     1741 gggagccgat tagggatgtg gcaagacccg gctccatcgc ccgcagtgag tttatacata
     1801 acaggttcta cggtcttgag gatacaaaca agtatctttg tgagcgcgcg aaagagtggc
     1861 tcaaagacgg ggatattgac tttgccttcc tgtatctggg ctgggttgac ggcgcgggtc
     1921 acgacttcgg ctggtgcagc gagggctata tgtatgccct taacaccacc tgcgacttgg
     1981 tttatgacct tgttcgggag ctgggggaca aatacaccat aattttcacc gctgaccacg
     2041 gcggacatga gagaacccac ggcaccgacc ttgacgagga catgaccata ccggtgttct
     2101 tcctcggcga gccctttgag aaaggcaaaa agctggaggg cgtcagcatt aaggatatag
     2161 ctcccacgat tgcgagcctt atgggagcca aaatccccat ggaatgggag ggtaaagttg
     2221 tcggatgaga aaataacggc agggggtaaa aggaaggctt taatgctcct ttttaccctt
     2281 gtctacttca caagctatgt aacaagaatc aactacgccg cgataatctc ggagattata
     2341 aggagcgagg ggatacttaa atccaccgcc tcccttgccc tgacgggcag ctccgtagcg
     2401 tacggaatcg ggcagatact cagcggctat ctgggcgaca aaatacagcc caaaacaatg
     2461 atttcggccg ggctgctcac caccattatg atgaaccttg ccgttccctt tttgggaaat
     2521 ccgtacctta cggcggcggt ttggacggtg aacggacttg cccaggcctt tatgtggccg
     2581 ccccttgtaa agataatgac ccatgtcttt actgaggacg actataaaaa ggcggttgtg
     2641 ctggtctctt ggggcagcag cttcggcacc gttgcggtgt atctgacagc gccccttttg
     2701 ataactctca gcggctggag agcggtattc gtgctctccg ctcttgcggc actcgttatg
     2761 ctgattttct ggcttatgaa gtgcccgaaa atcacccttg ccttaaagtc acagcccgaa
     2821 agcaaaaagg gcggcgcggc gcccttttct tttatgctct ttggaatcat cgccgccatt
     2881 gtccttcagg gctttttgcg tgacggagtt tccacatgga tgccgtcgct gatttccgag
     2941 acattcaatc tcggcaccaa tatatccata ttgacgggag tcggcttccc gcttttcagc
     3001 gtggtcgcct tctatgcggt ttccagaatc tacagggcaa gggtcagaaa cgagctgagt
     3061 cttgcgggaa ttatcttctt cttaggagcg ttctcggctc ttatgctgtc ggtactcagg
     3121 gacaaaaacg ccatacttac ggtgacgctt gtgtcgctgc ttatcggctc catgcacggc
     3181 gtgaatctga tgcttatcgg catggtgccc cgccactatg agaagttcgg cagaatatcc
     3241 ttgatgtcgg ggcttttcaa ttccttcact tatctcggca gcgccgcctc ggcgtacggc
     3301 atggcggcat ataccgagcg cttcggctgg aacagcatga tagtcttttg ggcagtcgtc
     3361 gccctcaccg gcggcataat atgcctctca ataaaaaaac cctggctcaa attcaccgga
     3421 agctgaaaga acccgacgct ctttgtcggg attttttctt ttgagcgctg tttaaagggc
     3481 tttgaatttt cccttccccg cataaaattt actgcgggcg gggcggcgaa atccttgact
     3541 tgaagcggga attggagtaa aatttagtgt actttagggc tttaagctgt taaagtaatt
     3601 tggctttagg aaagcaggga taatatgatt aaggctgcga tagtcggctt tggaaatgtg
     3661 ggcaggttca cttttgacgc gataatgaaa gctcccgaca tggagcttaa attcgttgta
     3721 tcttcaaagg accttgacat ccccgtgccg acggttaaga gcgttgatga actaaaggat
     3781 atagatgtgg cgattttgtg ccttcccagc aggaaggtgc cggaggtcgc cgaaaagctg
     3841 ctttcaaggg gcatttccac cgttgacagc tatgacatcc actcaaatat tgccgaggtg
     3901 cgtctgcgtc ttgacaagat agccaaggca aacggcgcaa aggctataat ctccgcgggc
     3961 tgggaccccg gcagcgactc ggttgtcagg gctctcatgc tggccatgat tccccgcggc
     4021 attacataca ccaatttcgg acccggcatg agcatgggac acaccgtcgc cgtaaaggcg
     4081 attgacggcg tcaaggacgc cctttccatg accattcccg caggctcggg cgttcacagg
     4141 cgcatggttt atgttgaatt gaaggacggc gccgatttcg agacagtcgc caaggctata
     4201 aaagcggacg actattttgt ccatgacgaa acccatgtaa tagaggttga cagcgttgag
     4261 gctttaaagg acatgggtca cggggtaaat attttcagga agggcgtttc cggctctacc
     4321 gacaatcaga gggttgagtt caacatgagc ataaacaacc ccgcccttac cgctcagatt
     4381 atggtttctt ccgccagggc tgttctgaag cagcagccgg gatgctatac aatgattcag
     4441 attcccccca ttgactacct ttgcggggac actgacgagc ttatcagagc gcttgtataa
     4501 aaaatgccgc agatttattc tgcggttttc tgctaaaggg gtgttttaat gtctaagaag
     4561 gctatagcgg cgatacttgt ctgcgtgatt ttggtctttt cgataggcgg ctttacggga
     4621 gcgaggatta taagaggcag agccctcagg atagtttccg ccgaaaagtc cgccatcata
     4681 aaagacggag aaacagtgca ttatgtggcg cacaggggct tttcgggcgc ggctcccgag
     4741 aacactctgc ccgcaatcga aaaggcgggc caggcgggat acaagtactg cgagtttgat
     4801 gtttatccca caaaagacag cgtatggatt ttgatgcacg acgaaaaaat ctcccgcacc
     4861 acgacgggca gcggctacat agaggactat acatacgagg agctgctaaa gtttgatatt
     4921 aagtacggca gcaatataaa ggattatcct tcccttaaaa tccccaccct cgatgaggct
     4981 ctggatgttt gcgaaaagta cggaatgcgc ccggttatcg agattaaggg cggcaaggct
     5041 gaggacttga aagttctgtg tgagttaatc gaaaagaaga atctcaaagc ggagcctgta
     5101 ttaatatcct ttaatttctc cttattgctg gagctgagga aactcactcc cgataccgag
     5161 atgtggctgc ttgtaagcga gattaaggag gacacccttt caaagtgcag ggagataaac
     5221 gcggggcttt catttgacgg aaagaacgaa aagaattacc cgaaaataaa accctttgtg
     5281 gacgagggaa ttctggtcgc cgcgtggacc attgacgata ttaagctcca cgacaccctt
     5341 tactctttgg gcgttcgata catcaccaca aacagaataa ccccgccgaa ttaagcggaa
     5401 aactcaaagg cagcttaggc tgcccttttt tattgccttc attttcatca attggtataa
     5461 agccctaaaa gcaaagcata ataaaatgag ccaatacagt ggttagggag atattatgct
     5521 gaacttttta aagaagctgt acgataaaaa gcaaaatgaa ctggaaaaga cggtaaacag
     5581 tttaaacgcc ccgctggaaa agctgaaggt aggggagacc cttgaggaga accttcaaat
     5641 ctgcaaaagg gtttttgcgg atatggatgt cattaccttt aaggaggttg cctgcgaggg
     5701 agagcagagt ttaaaatatc ttcttatcta ctgcgacggc atggttgaca ggggaattat
     5761 taacgacaat gtcttaaagc ccctgatgtc cgctcccccg gaggacacgg aagacctcat
     5821 aggcgcgctc aaaaaaagcg tcattcacgc taacgacgca atcagcaccg acagctttaa
     5881 ggatattgtg gagtttgtcg ccgccggcta tacggctgtg ttcgtggacg gcgtgggcga
     5941 ggctatcgcc ttaaatacca aggagtgctg cggcagagcc gtaatggagc ctgaaaacga
     6001 aaaggtcatc ggcggtccca aggagggctt taccgaggat attctgaaaa acctcgccct
     6061 cataataaaa cgagtgcgca ccaatgagct taaaaccaag tatctaaccg tcggcagaat
     6121 cacaaggacg aaaatctgcc tttgctattt cgacataata gcggacaaga atattctcaa
     6181 tcagcttata caaaagctta acaagctgga cattgacggc gttctggact ccaactatat
     6241 tgtggagctg ataagggaca accgttacag cgcgttcagg accgtgggct ataccgaaag
     6301 acccgatacc gccatagcca aaatgctgga gggccgcatt atcgtgcttg tggacggctc
     6361 ccccatggtg ctcagcatac cctatctgtt tgtggagaac tttcaaagca gcgaggacta
     6421 ttactttaac ttttattatt cctccttcgc gagaatcctt cgcatggcgg cgtttttgct
     6481 gaccatatca atacccgctt tctatatagc ggttgtcgcc taccatcatg agatgctgcc
     6541 gctgccgctg atgacgagaa tagccatgga gcagcagagg gtgcctttgc cctcagccat
     6601 tgaggctgtg attatgattt ttatctttga tatgctgagg gaaacgggtg tcagaatgcc
     6661 ttccagcata ggtcagaccc tcagtatcgt cggcgccctt gtcattggtc aggcggcggt
     6721 tgaggcgagt cttgtttccg ctcccatgat tatagtcgtc gccgctacgg gcattacaag
     6781 ccttctggtt ccgaaactca acgccccgat tatagtctac aggttcttct tcttgttcct
     6841 ttcctcagtc ttaggctttt tcggctttac catcgccatg agcattatga taatccacat
     6901 ccttaacctt acctcatcgg gcgtggacca ggttcagctt gacggctcct taaaactgca
     6961 taagtcaaag gatacctttg caagaatgcc ctggaatttt atgcagacca gacccgacag
     7021 gaccacagcc aacattaccc gtgaggcgag tgatttgtaa tgtttaaaaa gataaccgcc
     7081 ctgacgcttt ccttgtcttt tgtattcctc ataggctgct ggaattacag ggacctggat
     7141 gagatgaata tagtcgtggg tattgctctc gattatgaca gaatgacaaa ccaatacgcc
     7201 atgacgctgg aggtcgcgga tttgacgggc tcctccgacg aaagcggcat ttctggaaaa
     7261 atgatttaca ccaagggccg cacccttttt gaggcggcga ggaacacaaa gcgcagagag
     7321 caggacaagc tgtttttcgg cagcccccat gtgctgatta tcgaccagga actggcgtac
     7381 aagcagggca tagaggcggt catggagtgg tttatacgcg acgccgagtg ccgtgagtca
     7441 atctgcgtcg ccatatcccg cgcggaaacc gcgtcggaca tcttaacccc gccaaaggat
     7501 acaaagggtc ttgtatcgga aaggctgcac gacattataa gggaggacgg agaagtcaac
     7561 tccaccacat tcagagcgca gctttatcag atttataact ttctcaacgc tcccagacgc
     7621 tgcgcgatcc tgcccgcctt ccacagaacg caggacggtg aaagggtggt cccagaggtt
     7681 ctgggcgtag cggtaattaa gggcggcttt cttgtgggct ttttaagccc cgaggagaca
     7741 aaatatcttt tactggctga caacaaaatg aaaaccacaa tcctgcccat ttcggtaatg
     7801 ggcagaccca cagccgacgc cacgcttgaa gtgctggaaa accacagcaa aaaatccttt
     7861 aagtacgaaa acggacaact gaccctaata atcaaaaccg gcacgatttt ttcccttgcg
     7921 gaaaatcagg cccagctcga cattatggac aagaacaatg taaaaatgat tgaagacgcg
     7981 accaaggaag aaataaagta caacattgaa agtcttatga ataccttgca gaaggattta
     8041 aagaccgatg ttatgggctt ttcggaccat atttataaaa caaagcctcg gctttgggat
     8101 gagattaaag attattggga tgagctttat gagaacgcga aggtagaggt cgaggtcaag
     8161 gtgaaaatcg ttaactcggc atttacaaag taggggtagc atatgtgttt tttgttgata
     8221 gttgcttttt tcgcggttgt tctgatttgt gattccttaa caaaccttaa aggccttagc
     8281 aaagcgggca aggcggcgta ctttgttatg ttcgccttca gctttattgt gctttcaatc
     8341 tttaccttca accccgactt tactcccttt gcggggatta tagaaaagat tatagatatg
     8401 ttcgtggaag taaaaacaaa atagcgaaag ggacgaaata tggacaacaa gaccctaagc
     8461 ctcaatcagt cgatatgcgc cgtgctgctg tttaacttcg gcagcagcgt tattatgggc
     8521 atcagcacaa aggtagctca ggacgcgtgg atttccatta tcatagcaac tgttttgacc
     8581 atacctttgt ttatgatgta cggcaggatt ctcaataact ttcccggaaa gaatgtttat
     8641 gaaatttcgg aaacggtttt cgggaaagtg gggggaaaga ttgtttccgc ctttatggtg
     8701 agctactgca tatttctggc ttctttggta atgagaaact actcggagtt tactcagatc
     8761 ggcacccttg tggaaacgcc tcaggagccg atactgattc tgcttgtcat caccaccgtt
     8821 taccttgccc gcagcggcat aaagacagtg ggcaagtggt cggtgcttat gctgtttgtg
     8881 gttatctcgg tggttgtgct gatacttctg gtgtccatta atcagatgga gctgagctcg
     8941 atgcttccca tattcgagca ctcgttttcg gatattttct ggaccgctgt gcggataatc
     9001 tccttcccct acgctgagac agtggttttc ctgtgtcttg gctgctgctt tgccgcaaac
     9061 gaaagccgga caaagatttt cctgcgttcc ctttttttcg gcttcttcat tttcatgaat
     9121 atttcca
//
LOCUS       DAONVQ010000083         8996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5937_length_8996_cov_4.005033, whole genome shotgun sequence.
ACCESSION   DAONVQ010000083 DAONVQ010000000
VERSION     DAONVQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8996
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5937_length_8996_cov_4.005033"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1107)
                     /locus_tag="PLD98_06175"
     CDS             complement(97..1107)
                     /locus_tag="PLD98_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQA05869.1"
                     /translation="MNIFLIGGTGLLGCDAARMFIEQGHKVKSIALPPVPEGVELPEG
                     MELTFGSYLDFSDDDYREFFKDCDCFIFAAGIDDRVELPPPIYDEYVKYNIKPIDRIM
                     TIAKECGVKKAVILGSYFSYFAKKFPEMELTKKHPYIRSRIEQEKTALKFAEDGKMDV
                     AVLELPYIFGTQPGRKPVWVILVEQIANMPKVTLYPKGGTTMVTVRQVSQAIVGAATK
                     TKGGVAYPIGYYKLTWDELLKIVHEEMGFKNRKIIHIPKWMFKMYGKKLKKEYEEKNL
                     EPGLNPIDLADIMCMEAFIDKKWSEMLGVTEDDIESAIRDSIALSVEAMKGKELIGMK
                     AE"
     gene            1597..3927
                     /locus_tag="PLD98_06180"
     CDS             1597..3927
                     /locus_tag="PLD98_06180"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HQA05870.1"
                     /translation="MIKTIIKRDGREVEFSIDKIANAIFKAAQSVGGSNYDRAMEIAQ
                     HVVKKLEEKGLDKPTVEQVQDVVEQVLIEQGHARTAKKYILYRAERTKVREMNTRLMK
                     IYEGLTFDEAKKNDLKRENANIDGDTAMGTMLKYGSEGAKQFNELFILNPEHSRAHKN
                     GDIHIHDLDFLTLTTTCCQIDIIKLFEGGFSTGHGYLREPNDIASYSALACIAIQSNQ
                     NDQHGGQSIPNFDYGMAKGVAKTYRRTYLRNLGKAIDLLLDREDGQDIAKAAYEKLES
                     EHLYPVLANDNGYEEKLKEILISDHGLNEKQVDKILKFVLKHTVKETDRATYQAMEAL
                     VHNLNTMHSRAGAQIPFSSINYGMDTSPEGRMVIKNVLLATEAGLGNGETPVFPIHIF
                     RVKEGVNYNEGDPNYDLFKLACRVSAKRLFPNFSFMDAPFNKQYYVEGDYDTEVAYMG
                     CRTRVIGNYYDKTRQKTAGRGNLSFTSINLPRIAINAHGNLEWFFEELDRKIDLVFDQ
                     LLERLEIQSRKKVKNFPFLMGQGIWLDSEKLGINDEIREVLKHGTLSLGFIGLAETLK
                     ALVGAHHGESEEARNLGYEIVAHMRERCDKQTEKTGLNFTLLATPAEGLSGRFVKMDR
                     ERYGVIEGVTDRPYYTNSFHIPVYYPISAFEKIRIEAPFHALTNAGHITYVEMDGDPS
                     ENLEAFEAIIRCMKENNIGYGSINHHVDRDPVCGYIGIIGDKCPRHDGEGVPLEELRK
                     LPGYNPILNYKELMANGINPLEEFDKVTHPVDCCGK"
     gene            3968..4144
                     /gene="nrdD"
                     /locus_tag="PLD98_06185"
     CDS             3968..4144
                     /gene="nrdD"
                     /locus_tag="PLD98_06185"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HQA05871.1"
                     /translation="MSAKQNVIIGEGVRFERIRRITGYLVGTLDNFNDAKLAEVKDRV
                     KHTVDADSGNMERV"
     gene            4228..4737
                     /gene="nrdG"
                     /locus_tag="PLD98_06190"
     CDS             4228..4737
                     /gene="nrdG"
                     /locus_tag="PLD98_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HQA05872.1"
                     /translation="MENKLRLAGVIRESIVDGPGWRFVVFAQGCPHRCKGCHNPQTHD
                     FDGGYDTTVENIINEVKKNPYLKGVTLSGGEPFCQAKPLAVLAKKAHELGLDVITYTG
                     YTYEELIEGANEENGYIELLKETDILVDGRFILEEKTLELRFRGSRNQRVIDPKASLK
                     EGRVVETDF"
     gene            complement(4799..4987)
                     /gene="rpmB"
                     /locus_tag="PLD98_06195"
     CDS             complement(4799..4987)
                     /gene="rpmB"
                     /locus_tag="PLD98_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQA05873.1"
                     /translation="MAKCEFCGKAVAFGIQLSHSHRRSNRTWKPNVKNVRAIVNGTPK
                     RVNACTRCLRSGKVVRAV"
     gene            complement(5094..6011)
                     /locus_tag="PLD98_06200"
     CDS             complement(5094..6011)
                     /locus_tag="PLD98_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQA05874.1"
                     /translation="MKLLLIGVTLLSFTINQIAIRIFQVKYEHRGLSLYFFQFVFSML
                     ASMLCFHTAGNLSGPQNTTLLYGFLFGICYFLTVLLLSVCFSRGEMVIGSVIVNLSFI
                     VPIVYSLAVLNERITLTQIIGTILMGIAIYFSAADKRGEKGKCSQSVLLFALGAFLCN
                     GLSAVFQKHYRLTSSIDESMLFLSTGYLTAAMFFGFLFVFKRRSLLAPAPVIKEKAEE
                     NEEVSAYLKNIGVYLLAIVAAFSTFLGNGLIMCLSVAVPASILYPIINGGMTILIALC
                     SAFCFGEKLTKNKLLLLFFGILAIVVVNL"
     gene            complement(6015..7265)
                     /locus_tag="PLD98_06205"
     CDS             complement(6015..7265)
                     /locus_tag="PLD98_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA05875.1"
                     /translation="MDKKLRIGVFGAARGHSMISILLNHPHAELVAVCDKYEPLLMKA
                     KEAAEKAGLSITCYKTFDEFIQHDMDAVVLANYANEHAPYAIRCMKAGMHVLSEVLPC
                     ETMAQAVELIEAVEETGMVYAYAENYCYMKHAFEMWQRYQRGDIGEVMYGEGEYIHDC
                     SSIWPRITYGERNHWRNRMHANFYCTHSLGPLLTATGLRPVKVVGFETVPPENMIELG
                     AFRGTGIEIVTLENGAVLKSIHGDLKREPSSINYQLYGQKGMMETGRLEGDKLLNVYI
                     EGNERCKGTWENYDPVNTIAADFASKHSGHAGGDYYATHFFIEKILGNPDGKWSINVY
                     TAVDMGICGILAYRSVLNGNIPIDVPNLRLKEERDKYRNDNACTNPEIAGDDLLPISS
                     FGNPEFPDEVYDKVRQLRLEGKNA"
     gene            7546..8208
                     /gene="rpe"
                     /locus_tag="PLD98_06210"
     CDS             7546..8208
                     /gene="rpe"
                     /locus_tag="PLD98_06210"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQA05876.1"
                     /translation="MNLIAPSILSCDFSRIGEEVESCLNSGADLLHVDVMDGIFVRNI
                     TIGPPVVKAIRKCTPLKLDVHLMIDRPLRYIPEFLDAGSDIITFHAEADSPADEVIDL
                     IKKAGKTAGMAVSPNTPIDTVLPYLDRLGLVLIMSVEPGFGGQSFMPSVLPKVTALKE
                     EIERRGLNIDIEIDGGINDETIAQAKKAGANVFVSGSYIFKSEDRKAAIEKLRSIANG
                     FD"
     gene            complement(8224..8796)
                     /locus_tag="PLD98_06215"
     CDS             complement(8224..8796)
                     /locus_tag="PLD98_06215"
                     /inference="COORDINATES: protein motif:HMM:NF017226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HQA05877.1"
                     /translation="MRIEAHDDYRIAVELSDEDMDELEITYEDLDYSNIETRRVLWTI
                     LDEARQALGKEINLSEKMLIEAVPSGKGGCTIYFTVLQKPSEKNIQKLLVKKEVAPVI
                     CEFSDVNALIDLSRILIDKISGAKSELYIKDKTYRLLLFPETAYKKRIEAVASEFCDV
                     KESNHDTVSLTREHWKKLSGPYAVNEIGIY"
BASE COUNT         2557 a         2074 c         1997 g         2368 t
ORIGIN      
        1 catactcaag tgtggctagt tttgatatat gaagaaaaag ctattttcag aacagaaaag
       61 ggagcggcaa ttccgctccc ttaaaaatta taattcttat tcagctttca ttccgatgag
      121 ctctttgcct ttcatcgcct caacggaaag ggcgatggag tccctgattg cgctctctat
      181 gtcatcctcc gtaacgccca gcatttcgga ccacttttta tcaatgaagg cctccatgca
      241 cataatgtcc gcaaggtcaa tggggttaag accgggctca agattctttt cttcatattc
      301 cttcttcagc ttcttgccgt acatcttgaa catccatttg ggaatatgaa ttatctttct
      361 gttcttaaag cccatttcct catggacaat cttcaaaagc tcatcccagg taagtttata
      421 gtagcctatc gggtatgcca cgccgccttt tgtctttgtg gcggctccca caatagcctg
      481 agaaacctgc cttaccgtta ccatggtggt gccgcccttc gggtatagtg tgaccttggg
      541 catgttggct atttgctcga caagaattac ccaaacaggc tttctgcccg gctgagtgcc
      601 gaaaatatag ggcagctcaa gaaccgctac atccatcttg ccgtcctcag cgaatttgag
      661 ggcggtcttt tcctgctcaa ttctgctcct gatataagga tgtttctttg taagctccat
      721 ttcggggaac ttctttgcga aatacgagaa gtaggagccc agaatgacag ccttcttaac
      781 cccgcattcc tttgcgatgg tcattatcct gtcaatgggc ttgatattgt actttacata
      841 ctcgtcataa atcgggggcg gcagctcaac gcggtcgtca atgcccgcgg caaagataaa
      901 gcagtcgcag tccttgaaaa actctctgta atcatcgtcg ctgaaatcaa gatagctgcc
      961 gaaagtcagt tccattccct cggggagctc tactccttcc ggaacgggag ggagtgctat
     1021 tgactttact ttatggcctt gttcaatgaa cattcttgcg gcgtcgcatc ccaaaagacc
     1081 tgtaccgcca attaagaaaa tattcatttt tttctccttc tgttaatttt ttctatttga
     1141 cttaattata tcagaacttg tccgtacagt aaacaaaaaa atgtctaaga gcaaagtatt
     1201 ttctaaaatt tctcaacttg ctcttgcctt gtttgtggct gtcttatata ctatatatcg
     1261 tatcttaaat aaatatcccc ataggttttg tattttagtc acagatttgg caaagatata
     1321 atgtataatt tttacaacga ttctggtaaa tcaaaggcgt gctaagcccc caataatttt
     1381 ttgccgagcc gtttagcgta taatcgtgaa tttcgttccc attctgaaaa aatccatata
     1441 aattttttgt ccttttcttt attatcatgt cgaattgcac aaacattgta accgtttttt
     1501 taggggcaaa accattgact atttacaata tatgcggtaa aatacaggca ctcataaaaa
     1561 acaaacacaa tatatagtat atagtgggag ttagaaatga ttaagactat cattaagcgc
     1621 gacggtagag aggttgaatt ctctatcgat aaaatagcaa acgcaatatt taaggcagct
     1681 cagtctgtcg gcggcagcaa ttatgaccgc gcgatggaaa ttgctcagca tgtggtaaaa
     1741 aagctggaag aaaagggtct tgacaagccc acggttgagc aggttcagga tgtggtagag
     1801 caggttctta tagagcaggg tcacgcaaga acagccaaaa aatacattct ttatagggca
     1861 gagcgcacaa aggtcaggga aatgaacacc cgccttatga agatttatga gggtctgacc
     1921 tttgatgagg ctaaaaagaa cgacttaaag cgtgaaaacg ccaacatcga cggcgacacc
     1981 gccatgggca ccatgctcaa atacggctcc gagggcgcga agcagttcaa cgaactgttt
     2041 attttaaacc ccgagcactc cagagcccac aagaacggcg atattcacat tcacgacctt
     2101 gatttcctta cccttaccac aacctgctgc caaatcgaca ttatcaagct ctttgagggc
     2161 ggcttctcaa ccggtcacgg ctatttaaga gagcccaacg atattgccag ctattccgct
     2221 cttgcatgta ttgccataca gtccaaccag aacgaccagc acggcggtca gagcataccc
     2281 aatttcgact acggcatggc aaagggcgtc gcaaagactt accgcagaac atatctgaga
     2341 aatctgggca aggcaataga ccttcttctt gacagggagg acggtcagga tattgccaag
     2401 gccgcctatg aaaaattaga gagcgagcat ctctatcccg ttctcgcaaa cgacaacgga
     2461 tatgaggaga agcttaagga gattcttatc tccgaccacg gcttgaatga aaagcaggta
     2521 gacaaaatcc tcaaattcgt gcttaaacac acggttaaag agaccgacag ggcaacctat
     2581 caggccatgg aggcattggt tcacaacctt aacaccatgc actcaagggc aggcgcgcag
     2641 attcccttct cctccattaa ctacggtatg gacacctccc cagagggcag aatggttata
     2701 aagaatgtgc ttcttgcaac tgaagcaggt ctcggcaacg gagaaacccc cgttttcccg
     2761 attcatattt tcagggtaaa agagggcgtc aactacaacg aaggcgaccc caactacgat
     2821 ttgttcaagc tggcctgcag ggtatccgca aagaggctct tccccaactt ctccttcatg
     2881 gatgccccct tcaacaagca gtactatgtt gagggcgact atgacacaga agtagcctac
     2941 atgggatgcc gcacaagggt tattggcaac tattacgaca agacacgcca aaagacagcg
     3001 ggcagaggca atctcagctt tacatcaatt aacctgccga gaatcgcaat caatgcccac
     3061 ggcaaccttg agtggttctt tgaggagctt gacagaaaga tcgaccttgt atttgaccag
     3121 ctccttgagc gacttgaaat ccagtcgaga aaaaaggtta agaacttccc cttccttatg
     3181 ggtcagggta tttggcttga ctcagagaag ctcggcataa acgacgaaat cagagaggtc
     3241 ttaaagcacg gcaccctctc ccttggattt atcggtcttg ccgaaactct taaagctctt
     3301 gtcggcgctc accacggcga aagcgaagag gcaaggaatc tgggctatga aatcgttgcc
     3361 catatgagag agcgctgcga caagcagacc gagaaaacgg gtcttaactt caccctgctt
     3421 gcaacccccg cagagggact ttcgggcaga tttgttaaga tggacaggga gcgctacggc
     3481 gtaatcgagg gcgtaaccga cagaccctac tatacaaact ccttccacat tcccgtttac
     3541 tatcccatct ccgcatttga aaagattagg atagaagcgc ccttccacgc tcttacaaac
     3601 gcgggtcaca tcacctatgt ggagatggac ggcgacccga gcgagaacct tgaggcattt
     3661 gaggcaatca tcagatgcat gaaggagaac aatatcggct acggctccat caaccaccat
     3721 gttgaccgcg accccgtatg cggatatatc ggaatcatcg gcgacaagtg ccccagacac
     3781 gacggcgagg gcgttcccct tgaagaatta agaaagctcc ccggttacaa cccgatactc
     3841 aactacaagg agcttatggc taacggcatt aatccgcttg aggaatttga caaggtaact
     3901 catccggtag attgctgcgg aaaataactt aatttccgcg gtatacagtc aaaggaggaa
     3961 ttagagtatg tcagcaaagc aaaatgtaat tataggcgaa ggcgttcgtt ttgaaagaat
     4021 aagacgcatt acaggctacc ttgtaggaac tctggataac tttaacgacg caaagctggc
     4081 agaggtaaag gacagagtaa agcacaccgt tgacgcagac agcggtaaca tggagagagt
     4141 ataatcgccc agaaaaacgg ttttgcaaaa ggcacaggtc atcctgtgcc ttttaagtac
     4201 agaccgcagt aaagagagag tgacacaatg gaaaacaagc tccgtcttgc aggcgttata
     4261 agagaatcaa tcgttgacgg acccggctgg cgttttgtgg tctttgccca gggctgtcct
     4321 cacagatgca agggctgcca taacccccag acccatgatt ttgacggggg atatgacaca
     4381 acggttgaaa acataattaa tgaagtaaag aaaaacccct atcttaaagg cgtgacttta
     4441 agcgggggag agccattctg tcaggcaaag cccttagccg ttctcgcaaa aaaggctcat
     4501 gaacttgggc ttgatgtcat cacctacact ggctatacct atgaagagct tatagaaggc
     4561 gcaaacgaag aaaacggcta catcgaactg cttaaggaaa ccgacattct tgtcgacggc
     4621 agatttattc ttgaagagaa gacgctggag ctgaggttcc gcggctccag aaatcaaaga
     4681 gtaatcgacc ccaaggcatc acttaaagag ggcagggtag ttgaaacaga cttttaaata
     4741 aaaaagaaaa cgctccgaaa ggagcgtttt tcttataatc aattaattaa acttagcctt
     4801 aaacagctct tactaccttt ccggaacgga ggcagcgtgt gcaagcgtta acccttttgg
     4861 gtgttccgtt aactattgct cttacatttt taacattagg cttccaagtt ctgttagaac
     4921 gcctgtgtga gtgggataac tgtataccaa aggcaactgc tttgccgcaa aattcacatt
     4981 ttgccattat ggagtacacc tccttacaaa taaatcgaca aattgactgc acaagctata
     5041 atatcaaatc aaaggctata ttgcaagcat tttttatatt tatttaaagt tttctaaaga
     5101 tttacaacga ctatggcgag tattccgaag aaaagcagaa gaagcttgtt tttggtcagc
     5161 ttttcaccaa agcagaaagc ggagcaaagg gcaataagta ttgtcattcc gccgttgatt
     5221 atgggataga gaatcgacgc tggaaccgcg acgctcaggc acattataag accgttgccc
     5281 aaaaatgtgg aaaacgccgc cacaatcgca agtaagtata cgccgatgtt ctttaaatat
     5341 gcggaaacct cttcattttc ctccgctttt tcttttatca cgggggcggg agccaaaagc
     5401 gaccttcgct taaacacaaa gagaaaaccg aaaaacatgg ccgctgtcag atagcccgtt
     5461 gacaggaaaa gcatgctctc atcaatggag gaggtaagcc tgtaatgctt ttgaaaaact
     5521 gcggaaagcc cgttgcaaag gaaagcgccg agggcaaaaa gcagaaccga ttgcgagcat
     5581 ttgccctttt ctcctctttt atccgcagcc gaaaaatata tggctatgcc cattaaaatg
     5641 gtacctataa tctgtgtaag cgtaattctc tcattcagca cagccagaga gtaaacaatc
     5701 ggcactatga aacttagatt tacaattacg ctgccgatga ccatttcgcc ccttgaaaaa
     5761 cagacggaaa gaagaagtac cgttagaaag tagcaaatgc cgaacaggaa tccgtagaga
     5821 agggtagtgt tttgcggtcc gctaagattt cccgcagtgt gaaagcatag cattgaggcc
     5881 agcatggaga agacaaactg aaagaaataa agggataaac ctctatgctc gtacttcacc
     5941 tgaaagatac gaatcgcaat ctgatttatt gtaaatgata aaagggttac accaatcaaa
     6001 agcagtttca tattttacgc attctttcct tcgagccgga gctgtctgac tttgtcataa
     6061 acttcatccg ggaattcggg gttgccgaac gaagaaatgg gcagtaaatc gtcgcctgct
     6121 atttcgggat tcgtgcaggc attgtcattt ctgtacttgt ccctttcttc cttaagtctg
     6181 aggttcggga catcaatcgg gatatttcca ttaagcaccg agcggtatgc aagtatgccg
     6241 catatgccca tgtcgacggc ggtatagaca ttaatgctcc atttgccgtc ggggttgccc
     6301 agaatctttt ctataaagaa gtgagtggcg tagtagtcgc cgcccgcgtg tccgctgtgc
     6361 tttgaggcaa aatccgcagc aatcgtgttt actgggtcgt aattttccca agtacctttg
     6421 cagcgctcat ttccctcaat gtagacatta aggagcttgt cgccctccag tcttccggtc
     6481 tccatcattc ccttttgacc gtaaagctgg tagtttatgc ttgaaggctc tcttttaagg
     6541 tcgccgtgaa tgctctttaa aacagcgccg ttttcaaggg ttacaatctc gattcccgtt
     6601 cctctgaagg cacccagctc aatcatgttt tcggggggaa ccgtctcaaa gccaactacc
     6661 tttacgggtc tgagtcccgt tgcggtgagc aggggaccga gactgtgtgt gcagtaaaag
     6721 tttgcgtgca tgcggttgcg ccagtgattt ctctcaccgt atgtaatgcg tggccatatg
     6781 gaggagcagt cgtgaatata ctcgccctct ccgtacatga cctcgccgat atctcctctt
     6841 tgatacctct gccacatctc aaaggcgtgc ttcatgtagc agtagttctc agcgtaggca
     6901 taaaccatgc ccgtttcctc gaccgcctca atcagctcaa cagcctgagc cattgtttcg
     6961 catgggagca cttcgctcag cacatgcatt cccgccttca tgcagcggat agcgtaggga
     7021 gcgtgttcgt tagcgtagtt tgcgagaacc acagcgtcca tgtcgtgctg aataaattca
     7081 tcgaatgttt tgtagcaggt gatactaagc cctgcctttt cggcagcctc ttttgccttc
     7141 atgagcaaag gttcgtactt gtcacaaacc gccaccagct cagcgtgcgg gtgatttaat
     7201 agaatcgaaa tcatgctgtg gcctcttgct gccccgaata ctcctattct gagcttttta
     7261 tccatcggaa cgccccctgt ggtttgtttt ttcaactaaa actgtggtca aaagtagtat
     7321 aggaattatt taaaaaatag tcaacaataa actaaagctc agtgccaaag tcacaaacta
     7381 atttttgaca aataaaatgc cgatttcccc tttgtcagac cccaaaaaag tattgctatt
     7441 gacgaaaggg cttattgtga tacaataata tagttaaaca aggtgagtag tattttttgc
     7501 taaattctga gaaaattatt tacagaatac tttacggagg tagagatgaa ccttatcgcg
     7561 ccatccattc tttcatgtga cttttcaaga ataggcgagg aagtagaaag ctgcctaaac
     7621 tcaggggcgg atttgctcca tgttgatgtt atggacggca tctttgtgcg caatattacc
     7681 ataggtcctc ccgtcgtaaa ggcaataaga aagtgcactc ccttaaagct ggatgttcac
     7741 cttatgatag acagaccctt acgctatatt cccgagtttt tggacgcggg ttcggatatc
     7801 atcacattcc acgcggaggc ggactcaccc gcggatgaag taatcgattt aataaaaaaa
     7861 gcgggcaaga ctgcgggcat ggcagtgtcg ccaaacaccc cgatagacac agtcctgcct
     7921 tatcttgaca gattaggtct tgttctcatt atgagcgtgg aacccggctt tggcggccag
     7981 agctttatgc cgtcggtctt gccgaaagtc acggcattga aagaggaaat tgaaagacga
     8041 ggacttaata tcgatatcga aatcgacggc ggaataaacg acgagacaat cgctcaggca
     8101 aaaaaagccg gcgcaaatgt ctttgtttcc ggcagctaca ttttcaagtc cgaggacaga
     8161 aaagctgcaa tagagaaact aagatcaatt gcaaacggct ttgattaatt tgtttattta
     8221 aaattagtat atgcctattt catttaccgc ataggggccc gagagctttt tccaatgctc
     8281 tcttgtcaaa cttacggtgt cgtggtttga ttcctttaca tcgcaaaact cgcttgcaac
     8341 ggcttcaatc cgctttttat acgcggtttc gggaaagaga aggagcctgt atgttttgtc
     8401 tttgatatac agctcgcttt tggcgccgct tattttatcg atgagaattc ttgaaaggtc
     8461 aataagcgcg ttaacatcag agaattcgca aataacagga gcaacttcct ttttgacaag
     8521 cagcttttga atattctttt ccgaaggttt ttgaagcacc gtaaagtata tcgtgcagcc
     8581 tccctttccg gaggggacag cttcgataag cattttttca gagaggttta tttctttgcc
     8641 gagggcttgc cttgcttcat caagtattgt ccacagaacg cgtcttgttt cgatgtttga
     8701 ataatccaaa tcctcgtatg taatttcgag ttcatccata tcctcatcag acaattcaac
     8761 cgctattctg taatcgtcat gagcttcaat tctcatataa gccgccacct ctttgaacaa
     8821 attctttgat gttcttaaaa gacattttaa gcttcttgtg caccctttga tatactatta
     8881 tattatgctg actattctgt ttatgcaata ataaaaaact ggaaagcgaa gtgagagcct
     8941 tggacaagcc aaaggcttca ggcaaaaaga aggacacttt tataatagat gctctt
//
LOCUS       DAONVQ010000084         8969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_5950_length_8969_cov_5.461521, whole genome shotgun sequence.
ACCESSION   DAONVQ010000084 DAONVQ010000000
VERSION     DAONVQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5950_length_8969_cov_5.461521"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PLD98_06220"
     CDS             complement(<1..1056)
                     /locus_tag="PLD98_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQA05878.1"
                     /translation="MESIIINLSDSKPLYIQIYEHIRNGIINGDYRKNEPLPSIRGLA
                     KALSVSKITVEKAYDQLCSEGYIVGRERSRYYVAELSELTFFRPESVKSKKTPDTLDS
                     KKEYIFDFASGEMDSEGFDFALWRRYMGRAFSDVPRLMKYGDPSGEAELRREIAKYLN
                     HSRGVVTEPSNIIVGANTQVLIAQLCTLLNAEKDIIAFENPGFKNGRRIFTDYGYKVL
                     PISSENEGIDIDELKKSSAKAAYVSPSHQFPTGHIMPAPKRLALLKWAAESGAYVIED
                     DYDSEFRYYGNPIPAMKGMDTKDRVIYVGSFSKIIPPSIRISYMVLPENLLNAFKERI
                     SHYNQTASATEQLTLALY"
     gene            1161..1964
                     /gene="pdxK"
                     /locus_tag="PLD98_06225"
     CDS             1161..1964
                     /gene="pdxK"
                     /locus_tag="PLD98_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HQA05879.1"
                     /translation="MYKALTIAGSDTSGGAGLEADLKTFQEHSVYGMAAITVIVAQNP
                     KNNWAHDVYPIDLKTIEAQIDTVLGGIGVDALKTGMLPSEEIIRLTARKLREYGAKNI
                     VIDPVMVCKGTDEVVNPVAADAIKTELLPLADIVTPNIFEAAQLSGIRPIDSVEKVKE
                     AAKAIYELGAKNVLIKGGKKLGTKGAVDVFFDGKDFEILESPMIETTFTHGAGCTTAA
                     AITANLAKGASVKEAVHNAKDFITAAIEKGFRLNQYVGPVWHGAHREKI"
     gene            complement(2026..3006)
                     /locus_tag="PLD98_06230"
     CDS             complement(2026..3006)
                     /locus_tag="PLD98_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQA05880.1"
                     /translation="MKKNRLLSLIFTCLILGMMFMPKAKAAEAAAPTGRVTTASTALN
                     VRSGASTSAAVVASLAKDSYVRLLEKNGIWYKVEYSGGRYGYASASYITEVGSAKEAT
                     VNTSSGGLNVRTGPSTANGIKTVLAKGTAVVVLSESNGWANIVYNGTQTGYVSASYLK
                     YNTASVSSGYAAVKLSGVPNFKQTDSRWAAVTLGTSGKSIASIGCATTALAMTESYRL
                     GYTINPADMSKRLSYSASGSLYWPTNYVHDTGSGYLKTMYENLKKGIPTIYASTNAYG
                     GSHFVVVTGFKGGSLAASNFIINDPGSNSRVTLDQHIAAYPNFYKLVFYR"
     gene            complement(3361..4173)
                     /locus_tag="PLD98_06235"
     CDS             complement(3361..4173)
                     /locus_tag="PLD98_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQA05881.1"
                     /translation="MELTYYGCALITLKCGDFKLLLDPYFSRNPKTYSPEAEYFDDVD
                     AVLITHGHFDHIDDLPALTERNPNLPIYCTERVKKNLKTIGIKGGDFRLFKPGDSFKI
                     GPLNVKSIKSVHIKYDFAYIALHAVSPDFYRRFFKSFKAAADLKRSPIDGECLEYLIE
                     GDGESVLVAGSLGVDDTIKHPENVDVFVLPYNGRWIVNKQMYDHVKRIKPKKIFMSHF
                     DPAFPPMCLDAKPQRFVNFMRKMGENIDIIIPEYEKVYKVKEETAHKTAANL"
     gene            complement(4313..4654)
                     /gene="rplQ"
                     /locus_tag="PLD98_06240"
     CDS             complement(4313..4654)
                     /gene="rplQ"
                     /locus_tag="PLD98_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HQA05882.1"
                     /translation="MPGTRKLGRPTDHRKAMLKAMVTYLLENGRIETTVTRAKEVRSM
                     AEKMITLAKEDSLHNKRQALAYITKESVVKKLFDEIGPKYKDVNGGYVRIIRTGPRRG
                     DAAEMAIIELV"
     gene            complement(4690..5640)
                     /locus_tag="PLD98_06245"
     CDS             complement(4690..5640)
                     /locus_tag="PLD98_06245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQA05883.1"
                     /translation="MISIEKPRIETIELSEDGTHSAFIVEPLERGYGTTLGNSLRRVL
                     LSSLQGYAITSVKIDGILHEFSTIEGVKEDVTEIVLNLKSVILKINGNGPKTLYIDKQ
                     GPGILTAGDIQADSEVQIINPEMPIATLNEDAHVRMELTADAGRGYVSAERNKALLEP
                     AIGVIAIDSIYTPVKKVNYTVENTRVGHITDYDKLRLEVWTNGSITAKDAVSFAAKFL
                     NEHLNLFIDLSEEAGNAEIMVVKEDDDKEQILEMTVDELDLSVRSYNCLKRANIHTVG
                     DLINKTEEEMMRVRNLGRKSLDEVVSKLAALGLSLRREEY"
     gene            complement(5804..6427)
                     /gene="rpsD"
                     /locus_tag="PLD98_06250"
     CDS             complement(5804..6427)
                     /gene="rpsD"
                     /locus_tag="PLD98_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQA05884.1"
                     /translation="MSRYTGAVCKLCRREGKKLFLKGDRCYTHKCSIDRRNYAPGQHG
                     QNRRKSSEYGIQLRAKQQARRYYGIGESQFQKYFLMAERRKGITGENLLQICESRLDN
                     VVYSAGWANSRAEARQLVTHGHFLVNGQKVDIPSYLCKAGDVIAIKKSSLDSDKFKTL
                     LEANKRVIPLWIERSEDGAEAKIVNLPTRDQIELDVQEHLIVEYYSK"
     gene            complement(6452..6856)
                     /gene="rpsK"
                     /locus_tag="PLD98_06255"
     CDS             complement(6452..6856)
                     /gene="rpsK"
                     /locus_tag="PLD98_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQA05885.1"
                     /translation="MAAKGSAKKGTTRRRREKKNVERGAAHIQSTFNNTIVTITDLQG
                     NAVAWSSAGVNGFKGSRKSTPFAAQIAAEAAAKQAMESGMKTVEVYVKGPGAGREAAI
                     RALQTAGLEISLIKDVTPIPHNGCRPPKRRRV"
     gene            complement(6871..7239)
                     /gene="rpsM"
                     /locus_tag="PLD98_06260"
     CDS             complement(6871..7239)
                     /gene="rpsM"
                     /locus_tag="PLD98_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQA05886.1"
                     /translation="MARISGVDLPRDKRIEIALTYIYGIGRKVAGDIIKATGINPDTR
                     VKDLTEDDVSKLREYIDHNVKVEGDLRREVAFDIKRLVEIGCYRGVRHRKGLPVRGQR
                     TKTNARTRKGPKKTIANKKK"
     gene            complement(7272..7385)
                     /gene="rpmJ"
                     /locus_tag="PLD98_06265"
     CDS             complement(7272..7385)
                     /gene="rpmJ"
                     /locus_tag="PLD98_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HQA05887.1"
                     /translation="MKVKPSVKKICEKCKIIKRKGQVRVICENPKHKQRQG"
     gene            complement(7412..7630)
                     /gene="infA"
                     /locus_tag="PLD98_06270"
     CDS             complement(7412..7630)
                     /gene="infA"
                     /locus_tag="PLD98_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQA05888.1"
                     /translation="MAKQDMIEVEGKVVEALPNATFQVELENGRRILAHISGKLRMNF
                     IRILPGDTVTVEMSPYDLTRGRIIWRSK"
     gene            complement(7637..7969)
                     /locus_tag="PLD98_06275"
     CDS             complement(7637..7969)
                     /locus_tag="PLD98_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HQA05889.1"
                     /translation="MSHSNNAVMLWNGLCSEGVINLELTLGCIVRSKAGRDKGKFLVV
                     TAIEDNAVLLCDGKERPLERPKRKNIKHIAVTTAVLGADDMANNRALRRALRNYAQRT
                     TQKQDQGR"
     gene            complement(7945..8694)
                     /gene="map"
                     /locus_tag="PLD98_06280"
     CDS             complement(7945..8694)
                     /gene="map"
                     /locus_tag="PLD98_06280"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQA05890.1"
                     /translation="MIMLKSRRELEIMREAGRISARALQLAGEVIAPGISTADIDREV
                     RKFIESQGAVPSFLGYNGFPAAACISVNNEVIHGIPGKDRIIKEGDLVSVDLGASIDG
                     YHGDNAATFAVGKISPAAQRLMDTTREALYEGLKKAVAGGRIGDIGHAIESYCVSRGY
                     GIVREYTGHGIGRSLHEDPSVPNYGSPGKGTRLLQGMTLAIEPMINEGTGRIKLLGDG
                     WTVVTADGKLSAHFEHTIAITDGECLILTTP"
     gene            complement(8697..>8969)
                     /locus_tag="PLD98_06285"
     CDS             complement(8697..>8969)
                     /locus_tag="PLD98_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HQA05891.1"
                     /translation="SGRRVCLTCGTTYHIEYKPTREPDKCDVCGSVPVQRKDDSIETV
                     KERLRVYHEQTEPLIKYYEQKGKLRTVEGQERVEDTTALTLKALED"
BASE COUNT         2021 a         2188 c         2159 g         2601 t
ORIGIN      
        1 atagagggcg agagtaagct gctccgttgc agaggcggtt tgattgtagt gggatattct
       61 ttctttgaag gcatttagga gattttcggg caaaaccata tagctgattc ggatagaggg
      121 cgggatgatt tttgaaaagg agccgacata aataaccctg tcttttgtgt ccatgccttt
      181 catggcgggg atggggttgc cgtagtacct gaactcgctg tcgtagtcgt cctctatgac
      241 atacgcgccg ctctcagccg cccatttcag cagcgccagc cttttggggg cgggcattat
      301 atgccctgtg gggaactggt gggaggggct gacataagcc gccttcgcgc tgcttttttt
      361 aagctcgtca atatcaatgc cttcattttc ggagcttatg ggcagcacct tgtagccgta
      421 gtcggtgaaa atccgcctgc cgtttttaaa gccggggttc tcaaaggcga tgatgtcctt
      481 ttcggcatta aggagcgtac aaagctgggc tatgagtacc tgagtgttgg ctcccacgat
      541 gatgtttgaa ggctcggtaa caacgcccct tgagtggttg aggtattttg cgatttcccg
      601 acgcagctcc gcctcacccg acgggtcgcc gtatttcatg agcctcggca catccgaaaa
      661 cgccctgccc atataccttc tccaaagggc gaaatcaaaa ccctccgagt ccatttctcc
      721 ggaggcaaag tcaaagatat attccttttt gctgtcaagg gtgtcgggcg tcttttttga
      781 ctttacgctt tcgggacgga aaaaagtaag ctcggacagc tcagccacat aatagcggct
      841 tctttctctg cccactatat aaccctcgct gcaaagctgg tcgtaggctt tctcaaccgt
      901 aattttgctg acggaaagag ctttggcaag ccctctgatt gacggcagcg gctcgttttt
      961 gcggtaatcg ccgtttatta tgccgtttct tatatgctcg taaatctgaa tgtaaagggg
     1021 ctttgaatcg ctgagattaa ttattattga ttccataatc tgtccttatc aaaaataatg
     1081 aatttgtata ttttattaat gacactttaa ttatacaata aatttaaaat ttttccacta
     1141 tttttacttg ggaggtacat atgtacaagg cattgacgat tgccggctcc gacaccagcg
     1201 gcggagcagg tcttgaggca gaccttaaaa cttttcagga acactccgtc tacggcatgg
     1261 cggcaatcac cgtaattgtg gctcagaatc cgaaaaacaa ctgggctcat gatgtttacc
     1321 ccatagattt aaagacgatt gaggctcaga ttgacacggt tctgggcgga atcggcgttg
     1381 acgccttaaa gacgggtatg ctgcccagcg aggagattat ccgccttaca gcaaggaaat
     1441 taagagaata cggagctaaa aatatagtca ttgaccccgt tatggtctgc aagggcacag
     1501 acgaggttgt aaatcccgtc gccgcggacg ccattaagac cgagcttttg ccccttgccg
     1561 acattgtaac gcccaatatc tttgaagcgg ctcagctcag cggcataaga cccattgaca
     1621 gcgtagagaa ggttaaagaa gccgcgaagg caatttacga gctgggagcc aagaatgtcc
     1681 ttattaaagg cggaaagaag ctgggcacaa agggcgctgt ggatgtattc tttgacggca
     1741 aggattttga aatccttgaa agcccgatga ttgaaaccac attcacccac ggcgcgggct
     1801 gcactaccgc cgccgccatt accgcaaatc tggcaaaggg cgccagcgtc aaagaggcgg
     1861 tgcataacgc caaggatttc atcaccgccg caattgaaaa gggcttcagg ctcaatcagt
     1921 atgtaggtcc cgtatggcac ggcgcgcaca gagagaaaat ttaagcttgc tcctttatcc
     1981 ataaagaaaa ggcacagctt tttgggctgt gcctttttct ttgggttatc tgtagaaaac
     2041 cagtttgtag aagttggggt atgctgcgat gtgctggtca agtgttactc tgctgtttga
     2101 accggggtca ttgatgatga aattggaggc tgcgaggctg ccgcccttaa agcctgttac
     2161 aacaacaaag tgtgaaccgc cgtatgcgtt tgtggacgcg tagattgtgg gaataccctt
     2221 cttcaggttc tcgtacatgg tcttaagata gccgctgcct gtgtcatgga catagttggt
     2281 cggccagtag agtgagccgc ttgcagagta gctgagcctc ttgctcatgt cggcggggtt
     2341 gattgtgtag ccgagtctgt aggactcagt catggcaaga gcagttgttg cgcatccgat
     2401 ggaagcgatt gacttgcccg aagtgccgag agtaacagcg gcccagcggg agtctgtctg
     2461 cttgaagttc ggtacgccgc tgagttttac tgccgcataa ccgcttgaaa ctgatgctgt
     2521 gttgtattta agataagatg cgcttacata acctgtctgg gtgccgttgt agacgatatt
     2581 tgcccagccg tttgactctg agagcacaac aaccgcggtg ccctttgcga gcacggtctt
     2641 tatgccgtta gcggtggagg ggcctgttct tacattaagg ccgccggagc ttgtgttaac
     2701 cgtggcttcc tttgcggagc ctacctcggt tatgtagctc gcgctggcat aaccgtatct
     2761 gccgccggag tactcaacct tgtaccatat gccgttcttt tcaaggagcc ttacataaga
     2821 atccttagcg aggcttgcaa ctaccgccgc ggaggttgac gctcccgaac gaacattaag
     2881 agccgttgag gcggttgtaa ccctgcccgt gggcgcggcc gcttctgccg cctttgcctt
     2941 aggcataaac atcatgccaa ggattaaaca agtaaatatt aagcttaaaa gtctgttctt
     3001 cttcattgtc tcttttacca tcctttcaac gaagtaaact taaattttcc gtttctttgc
     3061 cgaccgtccg tcggcatatc cgactataac acacttaaat ctattttcaa cactccaaat
     3121 atttcaatgg aatttctata cttttttaat tggaaaattt ttcttaaagc aaaaatggca
     3181 aaaagacgga taatgccttg tatttcaggg gtttttgggg gtccggcctg agcgatcaga
     3241 aaagttttcc ttgagtcgtt tttgcgttaa aatgaaaaaa tttcctttga taatacgcgg
     3301 taaatcaatt tttaacaata aaaaagacag ccattcggct gccttaattt ctttgattta
     3361 ttacagattt gcggcggttt tatgcgcagt ttcttcttta accttatata ctttttcgta
     3421 ttcggggatt atgatgtcta tattttcgcc catttttctc atgaagttta caaagcgctg
     3481 aggctttgca tcaaggcaca tgggcgggaa cgcggggtca aagtgggaca tgaagatttt
     3541 tttaggttta atccttttta catggtcgta catctgcttg tttacaatcc atctgccgtt
     3601 gtagggcagg acgaaaacat ccacattttc cgggtgctta attgtgtcgt caacgccgag
     3661 gctgcctgct acgagcacgc tttccccgtc gccttcaatc agatattcaa ggcactcacc
     3721 gtcaatgggg cttcttttaa ggtctgccgc cgctttaaag gatttgaaaa accggcggta
     3781 aaagtccggg gaaacggcgt gaagggcgat atacgcaaag tcatacttta tatgtacgct
     3841 ctttatggat ttgacattta aggggccgat tttgaaactg tcgccgggtt taaagagcct
     3901 gaaatcgccg ccttttatgc cgatggtttt aagatttttc tttacccttt cggtgcagta
     3961 aataggaaga ttcggattgc gctcggttaa agcgggcagg tcgtcaatgt ggtcaaaatg
     4021 cccgtgggta atgagcactg cgtccacatc gtcaaagtac tccgcctcgg gggaataagt
     4081 ctttggattg cggctgaaat atgggtccag caatagctta aaatccccgc attttagcgt
     4141 aatcaacgcg cagccgtagt aggtaagctc caagaaaaat cattcctttc attttctcta
     4201 atgcctactt tagcataaaa taagttcaag acaaagacat tttgtgaaag gaaagtgtaa
     4261 agtttttttg aattttaaaa agggaacgga tttttccgtt ccctttatat cgttaaacca
     4321 gctctattat agccatctcg gcagcatcgc cacgacgcgg gcctgttctg attatacgga
     4381 catagcctcc gttaacatcc ttatacttgg ggccgatttc gtcaaacagc tttttgacga
     4441 cggactcctt agtgatataa gctaatgctt gccttttgtt gtgcaggctg tcttccttgg
     4501 caagggttat catcttctct gccattgagc ggacttcttt agctcttgtt acggttgttt
     4561 cgattctgcc gttttccaag aggtaagtta ccatggcttt aagcattgcc ttgcgatgat
     4621 cagtgggcct gccgagcttt ctggttcctg gcataaaatc actcctttac tctatgcggt
     4681 taaatctctt taatactcct cacggcgaag cgaaagtccc aatgcggcga gttttgaaac
     4741 tacctcatca agcgactttc tgcccaaatt ccgcacccgc atcatttctt cctctgtctt
     4801 gtttataaga tcgccgacag tatgaatgtt ggctctcttt aaacagttgt aggagcgtac
     4861 cgacaggtca agctcatcaa cggtcatctc aaggatttgc tctttgtcgt cgtcttcctt
     4921 gacgaccata atttctgcgt taccggcctc ctcggacaaa tcaatgaaga ggttgagatg
     4981 ttcgttgagg aacttggcag caaacgaaac cgcgtctttc gcggttattg aaccgtttgt
     5041 ccacacttca agcctgagct tatcatagtc agtaatgtga ccgacgcgtg tgttctccac
     5101 cgtatagttt acttttttaa cgggtgtgta aatcgagtct attgcaataa ctccgattgc
     5161 cggctcaagc aaagccttgt tgcgctctgc cgatacatag cctctgccgg cgtctgccgt
     5221 cagctccatg cggacatggg cgtcctcgtt aagagtggct atgggcattt cggggttgat
     5281 tatctgaacc tcagagtctg cctggatatc gcctgctgta aggattcccg gaccctgctt
     5341 gtctatataa agagtcttgg gaccgttgcc gtttatcttt aagataacgc tctttaaatt
     5401 cagaacgatt tccgtaacat cctccttaac tccctcaatt gtggaaaact cgtggaggat
     5461 tccgtcaatc ttaacggagg ttattgcata accctgcagc gaggataaaa gaactcggcg
     5521 aagactgttt cccaaagttg ttccgtatcc tctctcaaga ggctcaacga taaaggcgga
     5581 atgagtaccg tcctcactca gttcaatagt ttcgattctg ggcttctcaa tactaatcat
     5641 tcaaaaccct cctttaagga attaattaaa agcatgggga aatgctttta accgcatctg
     5701 ccgtccggaa atccattatt aaagttttga cagattttac ctgccttaac cacaaggaaa
     5761 accggacatg gcacaggatg ctccgcgctt tgtttaaagc ctcttatttg gagtagtact
     5821 caactatgag atgctcctga acatcaagtt caatctggtc gcgggtcgga agatttacga
     5881 tttttgcttc tgctccgtct tcggaccttt caatccagag gggtattact ctcttgttag
     5941 cctcaagcaa ggtcttgaac ttatcggaat caaggctgct cttcttaatc gcgataacat
     6001 cgcctgcctt gcaaaggtat gagggaatgt caaccttctg accgtttaca aggaagtggc
     6061 cgtgtgttac aagctgcctt gcttctgcgc gtgagttagc ccagcctgcg gaatatacta
     6121 cattgtcgag acggctctcg cagatttgaa gaaggttttc acctgtaatg ccctttctgc
     6181 gctcagccat caggaagtac ttttggaact gactctcgcc gatgccatag tagcgtcttg
     6241 cctgctgctt tgctctgagc tgaattccgt attcggatga ctttcttctg ttctggccat
     6301 gctgaccggg tgcgtagttt cttctatcga tggagcactt gtgggtgtag catctgtcgc
     6361 ctttcaggaa aagcttcttg ccttcgcggc ggcataattt gcatactgct ccggtatatc
     6421 ttgacatcta ataaaacacc tcctgttttg gttatacgcg tcttcttttg ggcggacggc
     6481 aaccgttatg gggaatcggg gtaacatcct taataaggct gatttcaagg ccggcggtct
     6541 gcaaagctct gattgcagcc tcgcgacctg cgccggggcc ctttacgtaa acctcaacag
     6601 ttttcatgcc tgattccata gcctgctttg cggcggcttc cgctgcgatt tgagcggcaa
     6661 agggagttga ctttctggaa cccttgaagc cgtttacgcc tgcgcttgac catgcaacgg
     6721 cgttgccctg caagtctgta attgttacga ttgtattgtt gaaggtagac tggatatgtg
     6781 cagcgccgcg ctcaacattc ttcttctcac ggcgtctgcg tgttgtacct ttctttgctg
     6841 aacctttagc agccacgcgt ttttacctcc ttacttcttc ttgttggcga tggtcttctt
     6901 ggggcctttg cgtgtgcgag cgttggtctt ggtacgctgg cctctgaccg gcaggccctt
     6961 acggtggcgg acgcctctgt agcagcctat ttcaacaagt ctcttgatgt cgaaagctac
     7021 ctcgcgcctg aggtcacctt caacctttac attatggtcg atgtattcac ggagcttgga
     7081 aacatcgtcc tcggtcaaat ccttaactct tgtgtccgga ttgattccgg tagctttgat
     7141 aatgtcgcct gcaaccttgc ggccgatgcc gtagatatag gtaagagcga tttctattct
     7201 cttatctctg ggcaggtcaa cgccggaaat tcgtgccatt tatcaaatgc acctccatta
     7261 attttacagg tttagccctg acgctgtttg tgcttgggat tttcgcagat aactctgact
     7321 tgtcccttgc gcttgattat cttgcatttt tcgcaaattt tcttaacaga aggtttgact
     7381 ttcattctgt tacctcctaa cttcagcttc gctacttgga acgccaaatg atgcgtcctc
     7441 gggtgagatc atagggcgac atttcaactg ttacagtatc cccggggaga atgcgtatga
     7501 agttcattct cagtttaccg gaaatgtggg caagtattct gcggccgttt tcgagctcta
     7561 cctgaaaggt tgcgttggga agcgcttcta cgactttacc ttcgacctct atcatatcct
     7621 gctttgccat aacttcttac ctcccctggt cttgtttttg tgttgttctt tgcgcataat
     7681 ttcttaaagc tctgcgcagc gcccgattat tcgccatatc atccgccccg agcaccgccg
     7741 ttgtcacggc gatatgcttg atgtttttcc gcttaggtct ttcaagcggc ctttcttttc
     7801 cgtcgcagag caatacggca ttgtcttcga ttgccgtaac cacaagaaat ttacctttgt
     7861 cacggcctgc tttcgaacgc actatacagc ccagagtcag ttccagatta atcactcctt
     7921 cgctgcaaag tccattccac agcattacgg cgttgttaga atgagacatt cgccgtctgt
     7981 aatcgcaatc gtgtgctcaa aatgcgccga gagcttgccg tcggcagtga ctaccgtcca
     8041 gccgtcgccg aggagcttta ttctccccgt gccctcgtta atcatgggtt cgatggccaa
     8101 tgtcataccc tgaagaagcc ttgttccctt gccgggagaa ccgtaattgg gtaccgaagg
     8161 gtcttcatgc agtgacctgc cgattccgtg acccgtatac tccctgacta tgccgtagcc
     8221 tctgctcaca caatagcttt ctatcgcatg gcctatatca ccgattctgc cgccggccac
     8281 cgcctttttg agtccctcat agagggcctc cctggtggta tccatcagcc gctgcgcagc
     8341 ggggcttatt ttgcccactg cgaatgtggc ggcgttatcg ccgtgataac cgtcaatcga
     8401 agcaccgagg tcaacgctga caaggtcgcc ttcttttatg atgcggtcct tgccgggaat
     8461 gccatgtatg acttcgttgt ttacggaaat acacgcagcc gcagggaagc cgttgtagcc
     8521 gaggaagctg gggacagcgc cctgactttc aataaacttt ctgacttcac ggtcaatgtc
     8581 cgccgtggaa atccccgggg ctatgacctc acctgcaagc tgtaatgctc tcgccgagat
     8641 ccggcctgct tcacgcatta tctccagctc gcggcgggac ttgagcatta tcatgtttag
     8701 tcctccaatg ctttgagcgt cagagcggtt gtgtcttcaa ccctttcctg accctctacg
     8761 gtgcgcagtt tgcccttttg ctcataatac tttatgaggg gctcggtctg ctcgtgataa
     8821 accctaagac gctccttaac ggtttcgatg gagtcgtcct tgcgctgtac gggaacggag
     8881 ccgcatacat cgcatttgtc gggctctctg gtcggcttat actctatatg ataggtagtg
     8941 ccgcaggtga ggcagactct gcgtccgct
//
LOCUS       DAONVQ010000085         8487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_6365_length_8487_cov_4.718809, whole genome shotgun sequence.
ACCESSION   DAONVQ010000085 DAONVQ010000000
VERSION     DAONVQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6365_length_8487_cov_4.718809"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLD98_06290"
     CDS             complement(<1..352)
                     /locus_tag="PLD98_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HQA05892.1"
                     /translation="MGPRGFPGRPGPAGPPGPAGPTGPTGPTGPTGPTGPEGGPPGPT
                     GPTGPTGPTGPTGPEGPIGPAGATGATGPTGPTGPEGPAGPTGPTGPEGPAGPTGATG
                     PEGPAGPTGPTGPEG"
     gene            complement(1007..1645)
                     /locus_tag="PLD98_06295"
     CDS             complement(1007..1645)
                     /locus_tag="PLD98_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQA05893.1"
                     /translation="MTYLIFAIVDWWIMQMMIFKGINRVTQTYGGSHGGIDIVGDTDP
                     TVRSVTDGEVYLVQYWDGKTKTGSQSYGNLVIVTHDGKRFYYAHLKSIAVKKNQQLKP
                     GDTIGIMGSTGNVTGPHVHFEVRTGSTTKTRVDPAPYCGVENKKGVYTEPVPHKYTAK
                     DARRALRIAATLETPTEEDYRLLDLDNDGKITAKDARDILRRAAELEEETNN"
     gene            complement(2041..2114)
                     /locus_tag="PLD98_06300"
     tRNA            complement(2041..2114)
                     /locus_tag="PLD98_06300"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2080..2082),aa:Gln,seq:ttg)
     gene            complement(2169..2245)
                     /locus_tag="PLD98_06305"
     tRNA            complement(2169..2245)
                     /locus_tag="PLD98_06305"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2209..2211),aa:His,seq:gtg)
     gene            complement(2252..2328)
                     /locus_tag="PLD98_06310"
     tRNA            complement(2252..2328)
                     /locus_tag="PLD98_06310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2292..2294),aa:Arg,seq:tct)
     gene            complement(2353..2429)
                     /locus_tag="PLD98_06315"
     tRNA            complement(2353..2429)
                     /locus_tag="PLD98_06315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2393..2395),aa:Arg,seq:acg)
     gene            complement(2605..2898)
                     /locus_tag="PLD98_06320"
     CDS             complement(2605..2898)
                     /locus_tag="PLD98_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQA05894.1"
                     /translation="MEKTLNKFINKEQLTANMAENLPVLRAKLGLTQEELSNLAGISR
                     HTVISVESKKRELSWSTFLALAFIFANNEATANLLNILGILNDDLMNYIKVSK"
     gene            3382..4047
                     /gene="yunB"
                     /locus_tag="PLD98_06325"
     CDS             3382..4047
                     /gene="yunB"
                     /locus_tag="PLD98_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HQA05895.1"
                     /translation="MNRRYGRRRPITVILYKFVVFLLIFAIGFLLVDARLRPLVKTLA
                     ANQAKNISIRIINDAVIDILQKTSYTYDDLVSLEKDDSGRITAIKTDSIKMNQLKSVI
                     STAITEEISKVEKRKVSIPMGNLTGLDSLAGRGPRISLYITLSGSTGAAIKNIFETAG
                     INQTRHQIVLEITTYIYAIMPGINTSTETSTNITIAETVIVGLVPEIYAGPNDNLWPD
                     LVK"
     gene            4233..5006
                     /locus_tag="PLD98_06330"
     CDS             4233..5006
                     /locus_tag="PLD98_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HQA05896.1"
                     /translation="MVVQIGMELYMTGIGTIVNSLAVLVGGSLGVLFNKNLKEKYQEI
                     LLQALGISVIFVGLTGSLSGLFYVEGSVIKTKDIMLLILSLAIGSLFGELIGIEKGIE
                     NVGEKIKKKFSKGGENDTFIEGFVSATLLVCVGAMAVVGSFEDGLKGDPTMLFTKSIL
                     DFASIMVIASALGKGAVFSAFPMFLYQGSLTLLAKIVAPLLTDAIVGSLSTVGSALIF
                     AIGINMCFGKKFRVGNMLPSLLVVLIYYGGKALFGHFFS"
     gene            5067..5606
                     /locus_tag="PLD98_06335"
     CDS             5067..5606
                     /locus_tag="PLD98_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HQA05897.1"
                     /translation="MEFKGSRTEANLMAAFAGESQARVKYGYYASQAKKEGYEQISAI
                     FTETSDNEKEHAKLWFKELHGGKIPSTMENLHDAAAGENYEWTDMYKEFERVAREEGF
                     DRIANLFKLVGSVEQNHEKRYRKLIENLEKGLVFEKTGEVVWKCRNCGYIHVGKAAPV
                     VCPTCAHPQAFFEVLAENY"
     gene            complement(6424..7365)
                     /locus_tag="PLD98_06340"
     CDS             complement(6424..7365)
                     /locus_tag="PLD98_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05898.1"
                     /translation="MRRYKRDASCAKLILKKSGGYLRISIMFLVSVVITSIIGLLYAR
                     QYFQFEKDFLTNIALRTFVVDANYGENRIGRLNQEDVEQISRLLKEKFPNEEFSVIPV
                     YRNSAVGLFGRYVRLYGIDPKHSFIIGLENMEDNVLYTVSNMQDTVPLEISVVKIEEN
                     GFEAGENHTKSMKTSTGVSKNSPVLSDDLMRDSDMEYCFVTTKTFEEIIPITLDGRAK
                     SIKEALEMSASLVEIRSIYVVAENILMLNPAYSHLIEHNYRSYAKIDVFEDFSETISV
                     SYLVYFCSSFVLLIMTTVNIILSFRSFYRVQQKDMAF"
     gene            complement(7362..7565)
                     /locus_tag="PLD98_06345"
     CDS             complement(7362..7565)
                     /locus_tag="PLD98_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05899.1"
                     /translation="MAMVSLSDPDIILLDEPTAKLDDYNISLTLEAIERVWQNKLVLI
                     VSHDKLLFSVPFTELCLEGGKLR"
     gene            complement(7582..7962)
                     /locus_tag="PLD98_06350"
     CDS             complement(7582..7962)
                     /locus_tag="PLD98_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA05900.1"
                     /translation="MAFIQLRDLSLSFGEKKVLDSITYTFKDGITVLHGESGSGKTTL
                     LHCIAGIYKNYTGEIILNPPEKPAYCMQEDLFFSNLTVEDNMHLKYVALNKPADKEKE
                     LVIEALSMFGIDSLIKSPAKNLSG"
     gene            complement(8259..>8487)
                     /locus_tag="PLD98_06355"
     CDS             complement(8259..>8487)
                     /locus_tag="PLD98_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05901.1"
                     /translation="DFKGNVSIPKDELQHCFPEFKPYLQDCRFSGCAHLKDKDCKVRE
                     AAENGEIPMERYRSYQELYLEAKEKEDEMYR"
BASE COUNT         2313 a         1835 c         1856 g         2483 t
ORIGIN      
        1 gaccttcggg acctgtggga cctgtgggac ctgcgggacc ttcaggacct gttgcgcctg
       61 tcggacctgc ggggccttcg gggcctgtcg gaccagtcgg gcctgcggga ccttcaggac
      121 cggtaggacc tgtgggacct gtagcacctg ttgcgcctgc gggacctatg gggccttcgg
      181 gaccagtcgg gccggtggga ccggtaggac ccgtgggacc tgtcgggccg ggcgggccgc
      241 cttcaggacc tgtaggaccg gtgggacctg tcggacctgt cgggccggta ggacctgcgg
      301 gaccgggggg acctgcagga ccgggtctgc ccggaaatcc tctcggaccc atcggacctg
      361 tcggaccggt cggacccgtt gggcctctga ggcaacagca tttgtctata ggttgtgcgc
      421 agactttgca tttcggcttt tcgcagcagc aatcatcata ttttgagtaa ttgccgcacg
      481 aacaagacgg atcgtatgag taatcggtac cgtaatagta tctcatttta tccctacctt
      541 cttgtagtat agattagcag tctgttaccc caaacctgta ttacttgagg atataggcta
      601 tgttcctata cattttcagt atatgccttt tcttccaaag ggggactacc ttatataact
      661 gcttcattat aggttactaa gcaaatgtaa attaggtgat tgttttttaa gatttagctg
      721 agatttttga tagacacgct ttctttgaac tcgtgcccgc ttttgatttt ggatggtttg
      781 agaaaattat tcggagagca agcaatccaa cgattaatac tattgttgag taaataaaag
      841 ctttaaatga ctttatctgc aaggatgaat tcctcggcat atgtaaccga attgttgtgc
      901 ttgtccgcct tgcggtcggg caattcaaga cggtaattta caaggctttt tagagctaca
      961 tgcccgcatt aaccgaaaat tgtgcccaac ggcgaaaata aagcaatcaa ttattagtct
     1021 cttcttcgag ctccgccgcc ctcctaagaa tatcccttgc gtctttggcg gtgatttttc
     1081 cgtcattgtc aaggtctaag agtctgtaat cttcctctgt cggcgtttca agcgttgccg
     1141 ctattcttaa agcccttctt gcgtctttgg cagtgtattt atgaggtacg ggctcggtat
     1201 atactccctt tttgttctca acgccgcaat acggggcagg gtcaacacgg gtcttcgtcg
     1261 ttgagcctgt gcgcacctca aaatggacat gcggaccggt gacattgccc gtgctgccca
     1321 taatacctat ggtgtcgccc ggcttgagct gttggttttt ctttacggca atactcttga
     1381 gatgggcgta atagaaccgc ttgccatcat gagtaacaat tacaaggttt ccgtagctct
     1441 ggctaccggt ttttgttttg ccgtcccaat actgaaccaa atatacttcc ccatcggtta
     1501 cgcttctgac ggtcgggtca gtgtcgccaa caatgtctat tccgccgtgg ctgccgccgt
     1561 aggtctgcgt tacacggtta atgcctttga atatcatcat ttgcattatc caccaatcta
     1621 cgattgcaaa aattaaatat gtcaatcaaa gcctgctttt tcccgcggca gacttaaatt
     1681 gcgcgtctgt tctgcaaact cttatttatg tctgctttat attcatatgt cgtaaaggcg
     1741 ggtattgtgt aagctatttg cagataagac aagctaaaaa tttcgaccaa aagcggggaa
     1801 aggaagcggt gtgcgtaaag aaaaatctac gccttaaaat ggtgcgattt attgggcatc
     1861 acccttcatt aaatcgcgga atcacacaaa aacaaaaagg agggctatta aagcccctct
     1921 taaaacaagg aaatacaaaa tgcgtacttg tacctgttcc cgccgataat cgtggatttt
     1981 tcgtgtattc acaaaaagag agacccgtaa gccttgataa atataggctt acgggtcttt
     2041 tggctgggat ggctgggatc gaaccagcga tgacggagtc aaagtccgtt gccttaccac
     2101 ttggctacac cccaaaagga gttataaaaa taaaagcaag tttaaaactt gccgtcttaa
     2161 aaaataaatg gggtggataa tcggactcga accgatggcc tccagggcca caacctggcg
     2221 ctctaaccaa ctgagctata cccaccatat gtggcgcgct tgaagggatt cgaacccctg
     2281 acccactgct tagaaggcag ttgctctatc ctactgagct acaagcgcag atgtaaaaat
     2341 gcatagcgaa actggtacgc caggagggat tcgaaccccc gaccttttga ttcgtagtca
     2401 aacactctat ccaactgagc tactggcgca gatacagtgc cgctacgcat tttttacgac
     2461 ggactcaagg accgtcgcca aagtatatta tcaccaatta gctgtgttgt caatatgtaa
     2521 taccagaatt tatatttttc cagatgcttt taaattgttt actatgctat attagcacac
     2581 aaaagccgcc ctctttaaga aagcttattt tgaaaccttt atgtagttca taaggtcatc
     2641 gtttagtatt cccaatatat ttaaaaggtt tgctgtagcc tcattattcg caaaaatgaa
     2701 agccagagcc aagaaagtgc tccacgacag ctccctcttt ttgctttcca ctgaaattac
     2761 ggtatgcctg ctaataccgg caaggtttga aagctcctct tgtgtaagac cgagctttgc
     2821 tctgagtaca ggcagattct ctgccatatt tgctgtgagc tgttctttgt tgatgaattt
     2881 gttaagcgtc ttttccacgc tcttctacct ccgaaaccta tattgccaag ccttatgcaa
     2941 ataattctca agctcaaggc tcagccttcc tactagctta aatctaacta attcgctact
     3001 aaactaaagc cgaaatgcca aaaaccgacg catttagcgg ctgggatttt tatgagtaat
     3061 tatacactgc taaccttgct atgtcaatga aaacaagacc agaaaaacta aaatttaata
     3121 aaaattcata ctttgcaaat cgcatatgac acaaaattcc tttagttgca aaaataccaa
     3181 ttttctcctg tgcggcggca ggtttttgat tttgcccctg tatcaaaatg caaaaatatt
     3241 ctgtatattt gctaatactg cgtattttta ttcactgcat caagcaaaaa gaatttgaca
     3301 tgagtttatt ccattggcac aatttcagct tgaccgcata tactgtaaag agcggtaaaa
     3361 tttttgcatg cggtgatttt tttgaacaga agatatggca ggagaaggcc gattactgta
     3421 atactatata aatttgtagt tttcttgctt atctttgcga taggctttct tcttgttgac
     3481 gctcggctca ggcctcttgt caaaactctt gccgcaaacc aagccaagaa catatccata
     3541 agaataatca acgacgcggt tattgatata cttcaaaaaa cctcatatac atacgacgat
     3601 ttggtatccc ttgaaaagga tgattcgggc aggattacgg cgattaagac ggacagtata
     3661 aaaatgaatc agctcaaatc cgttatcagc accgcaatta ccgaggaaat aagcaaggtt
     3721 gagaaaagaa aggtaagcat acccatgggc aaccttacgg gtcttgattc attggcgggc
     3781 aggggtccga gaataagtct ttacatcacc ctttctggca gcaccggcgc tgcgataaag
     3841 aatatatttg aaaccgcggg cataaaccag acaaggcacc agattgtgct tgagattacc
     3901 acctacatat acgccattat gcccggaata aatacatcga ccgaaaccag cacaaacatc
     3961 acaattgctg aaacggtaat agtaggtctt gtacccgaga tttacgcggg tccgaacgac
     4021 aatttgtggc cggaccttgt aaaataactt tcagaattaa tatccataaa ccgaccgttc
     4081 tttcaaagaa cggtttatta ttttttatat cggtcaataa tctttttgag tttttgcttc
     4141 gctccatgac tccgatagga cagaagggta aatttaatca cttgcgctta gcaaattttg
     4201 gagttataat tatttaaact ggattattag aaatggttgt tcaaatcgga atggagcttt
     4261 atatgaccgg cataggaacc atagtaaact ccctggcggt gctcgtaggc ggcagcctcg
     4321 gagtgttgtt caacaaaaac ttaaaagaaa agtatcagga aattcttctt caggctctgg
     4381 gtatttcggt aatattcgta ggtctcacag gcagcctttc gggtctcttt tatgtagaag
     4441 gctctgttat taagactaag gatataatgc ttttgattct ttcccttgcg ataggctcat
     4501 tattcggtga gcttataggc attgaaaaag gcattgaaaa tgtaggcgag aagataaaga
     4561 agaagttcag caaaggcgga gagaacgaca cctttataga gggatttgtg tccgctactt
     4621 tgctggtatg cgtgggagcc atggcggttg tcggctcttt tgaggatgga ttaaagggcg
     4681 accccacaat gcttttcact aagtctattc tggactttgc cagcattatg gtcatagcct
     4741 ccgccttggg aaagggcgcg gttttctcag ccttccccat gttcctttat caaggctcgc
     4801 tgacgcttct cgcaaagatt gtggctcccc ttttaaccga cgccatcgtg ggttcattgt
     4861 ccacggtcgg ctcggctcta atctttgcga taggcatcaa tatgtgcttt ggcaaaaagt
     4921 ttagggtggg caatatgctc ccctccctgc tcgtggtgct tatctactac ggcggcaagg
     4981 cattgttcgg gcacttcttt tcttaacagg taacaaccaa tgtttacatt ggcatttata
     5041 catctaatat aaggaggaat aaaacaatgg aattcaaagg cagcagaact gaagccaatc
     5101 ttatggcggc attcgcaggc gagtctcagg caagagtaaa gtacggatat tatgcttctc
     5161 aggcaaagaa agaaggctat gagcagattt cagcaatatt caccgaaaca tccgacaatg
     5221 aaaaggaaca cgcaaagctt tggttcaaag agctccacgg cggcaagatt ccctcaacaa
     5281 tggagaacct tcacgacgca gccgcaggcg agaactatga gtggaccgat atgtacaagg
     5341 agtttgagcg tgtagcaaga gaagagggct ttgacagaat agctaacctc tttaagctcg
     5401 tcggctccgt tgagcagaac catgagaagc gttaccgcaa gctgattgag aaccttgaaa
     5461 agggtcttgt atttgaaaag accggtgagg ttgtctggaa gtgcagaaac tgcggttaca
     5521 tccatgttgg caaggcagct cccgttgtat gccccacctg cgcacatccc caggcattct
     5581 ttgaggttct tgctgagaac tattaatccc aaaattaaaa tgctctcccg tatgggggag
     5641 cattttttct tgagatctgg ctgagaactt ttaatctgtt gaattaaatg ctctaccgaa
     5701 atggggagca tttttttgcg cttagctttt caaattcaga aattaggaac ctttaggcat
     5761 actgctgcgt ataattaaat tagacactga aaaggaaggc aggcgatgcc attgagattc
     5821 aggcgtgcga actttggcac cgttttgatc gcattcggag ccggtctttt tttggcgttc
     5881 tgctttccgt ctcattatct tgtaataatt cttgcagtca tagtattctt atgcggtctt
     5941 ttgattaaca gaaattcatg tgggggttaa tttatgaaag ttgttgttat tacaagtccg
     6001 aagatattaa ggggcgtatt aagacttatt ttcggaataa aaaagcaggc atcgcccgga
     6061 cagtaatctg tagttaaact tacatagctc ttccaaagat aaaacggcaa gtcttaaaaa
     6121 cttgccgttt atctttttta tttaggggct attcaaattc tttggattct ctaataagca
     6181 cgagaaaatc ctgctttatt tacttgaaaa ctatagatct tgaaacaaca aaatagataa
     6241 agctaaagta tatgcaaagc gcagcgataa agcccgcaag tacaatgtag tgttcgaaac
     6301 taaataagaa cagtcccgta acgaaaacta ttgcggagga gataagcagg atttgaataa
     6361 actttttgcg gagattgcgc ttgaacattt tatataccct cttaatatca aatcccatgt
     6421 gccttaaaat gccatatcct tctgctgcac tctgtagaaa gacctgaaag aaagaatgat
     6481 gtttacggtt gtcataatca gaagcacaaa ggaggagcaa aagtaaacca agtaggatac
     6541 tgaaatggtt tcgctaaagt cctcaaatac atctattttg gcatagcttc tgtaattatg
     6601 ctcaatcaag tgggaatacg cagggttgag cattaatata ttttccgcaa caacatatat
     6661 tgaccgaatt tcaaccagac tcgcggacat ttcaagagct tccttaatac tctttgccct
     6721 tccgtccaaa gtaatgggaa taatctcctc gaaggttttg gttgttacaa aacagtactc
     6781 catatctgaa tcccgcatta aatcatctga caatacgggg gagttttttg aaacacccgt
     6841 gcttgttttc attgattttg tatgattttc gcccgcttca aacccatttt cctcaatttt
     6901 aactacagat atttcaaggg gtactgtatc ctgcatattg ctgacggtgt aaagaacatt
     6961 gtcctccatg ttttcgagcc cgatgataaa actgtgcttt gggtcgatgc catataacct
     7021 aacatatctg ccaaataaac caactgctga atttcgatat acgggaatga cgctgaactc
     7081 ctcgttagga aacttctcct tcaataacct gctgatttgt tcaacatcct cttgatttaa
     7141 tctgccgatt ctgttttcgc cgtaattcgc gtccacgaca aaggtacgaa gcgcgatgtt
     7201 cgtaagaaaa tccttttcaa attggaaata ctgccttgcg tacaaaagcc caattatgct
     7261 ggttatcact acggatacca agaacataat tgatattctt aaatatccgc cggacttttt
     7321 gagtatgagc tttgcgcagg acgcgtcgcg cttatacctt ctcatctgag ttttcctcct
     7381 tcaaggcaaa gctcggtaaa cggtacagag aataacagct tgtcgtggct tactattaaa
     7441 accagcttat tctgccaaac ccgctctatg gcttcaaggg ttaggctgat gttgtagtca
     7501 tccagctttg ccgttggctc gtcaaggagg attatatctg ggtcggacaa cgacaccatg
     7561 gccattttta agcgctgacg ctcagcccga aaggtttttg gcagggcttt ttataagaga
     7621 atctatgccg aacattgaaa gtgcttctat taccagctct ttttctttgt cggcaggctt
     7681 attcagggca acatacttta ggtgcatatt atcctcgacg gtcagattgc tgaagaacaa
     7741 atcctcctgc atacagtagg cgggtttttc gggcgggttt aagattattt cgcccgtata
     7801 gtttttgtag attcccgcaa tgcagtgaag cagggttgtt ttgccggagc ccgattctcc
     7861 gtgcagaacg gtaatgccgt ctttaaaggt gtatgtaatc gaatcaagta ccttcttttc
     7921 cccaaaggaa agggataaat ctctaagttg aataaaagcc atgtcattct cccttttcaa
     7981 tttcaagatt ttttatatag ccttcttcga aaacggtaat ttcggagttg cttaattttt
     8041 gccaatgcgt tttcgtatgt ggctaagaca cttcccgttc ggttgagatt atatccgggg
     8101 atggaaatta attggaaact atgtaacaat tatacataat tctcgcaaaa ttgcaattaa
     8161 tttttgggat tttttacaag ttttcagtca aactcacgca taataaaaac agccgcaaag
     8221 gctttgcgcc ttttgcggct gttgtatttt tgtttaacct atctgtacat ttcgtcttct
     8281 ttttctttag cctcaaggta aagctcctga tagcttctgt acctttccat cgggatttcc
     8341 ccgttttcgg ctgcctctct gactttgcag tctttatcct ttaaatgggc gcagcccgaa
     8401 aagcggcaat cctgaaggta gggtttgaac tcagggaaac aatgctggag ttcgtccttg
     8461 gggatggata cattgccctt aaagtca
//
LOCUS       DAONVQ010000086         8459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_6391_length_8459_cov_4.534626, whole genome shotgun sequence.
ACCESSION   DAONVQ010000086 DAONVQ010000000
VERSION     DAONVQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6391_length_8459_cov_4.534626"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1187
                     /locus_tag="PLD98_06360"
     CDS             117..1187
                     /locus_tag="PLD98_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQA05902.1"
                     /translation="MKNTELFTEQKIIKGKNGLAALFLILLALLAFTALGIAGIVIAS
                     RAARYGSIIMPYSGPSENFGGALIVISIIGFVASTIMFAGLKTLKPNEALVLTLFGKY
                     FGTLEGPGFFFVNPFVTAVNPIKRTPIAVNTAGTVDLQPKPAQRGEKMDLIDSASGKA
                     ISLKIMTLDNNKQKINDKLGNPIEIGVVIVWRVVNAAKAVFNVDNYFTYLSTQADSSL
                     RNIVRLFPYDVWQDGEEADEMSLRGSSQEVAVKLREELQSKVEIAGLEIVEARITHLA
                     YAPEIAAAMLQRQQASAIIDARKLIVEGAVGMVEMALQKLSEQNIVELDAERKAAMVS
                     NLLVVLCGNKEAQPIVNSGSIY"
     gene            1193..1354
                     /locus_tag="PLD98_06365"
     CDS             1193..1354
                     /locus_tag="PLD98_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05903.1"
                     /translation="MSSNKKQIPLRLSPELYEKLAAWAEDEFRSLNGQIEYLLTECVK
                     KRYGEKKDK"
     gene            complement(1487..2968)
                     /locus_tag="PLD98_06370"
     CDS             complement(1487..2968)
                     /locus_tag="PLD98_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA05904.1"
                     /translation="MDIQTLAKDTYTRRYVNRKETIAYVLFDSSKSFNIDEYQTRFII
                     DVLKIDLYWNTIINFINGIWDVINDSILGSLIDKTKTRWGKFRPYLLAYALPGTALTC
                     LYWLSPLFFDKNPLDMRKIAFWLILAMAREAVGTLREISETGLLANITPNPEDRVRLY
                     TTAEVVSGLWESFPQIIMGALIDLVNHKKIKMTMDNAFIGMGTFCTISGGILALFFSI
                     YSTERIAQSRERHNYKEGFRTIINNKPMLIILVSDLISKFPVGVWEHNYFIDVLGSSS
                     LRNVIIIPGAPLSILSYTYINQVRARFSTKSLWIFGQHLKDVITLGIFVIGSMRGIGP
                     KGIYQNRLIMTVLLMVRDIFYKGTLSINKILPREILTDALDYCEWKFGYRTEGVTLAT
                     KSMAYKLFRNVTNSFNSAIMKKAGYSLDAGFGKQSEKTKYFLFAMSMLMPAATGLLGI
                     IPKLFYDLSGDKRRRMYEELAKMRLEKMNAYERENKEQVLTEV"
     gene            complement(3003..3743)
                     /locus_tag="PLD98_06375"
     CDS             complement(3003..3743)
                     /locus_tag="PLD98_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQA05905.1"
                     /translation="MKNEKEIDKTFSLISGFGVKHIELAVDYLKMPFTAKTAELIANA
                     AKAHGIETVSCQIRYNTAAKDTGLTAEFMHILGARFISNSVIDLSALRKGKEGVLRYC
                     EQLNSLYEKLKRQDILLSHHNHHYEFLKFDGKTVLELMRENFSGLFTVDTYWCQKGGG
                     NILTLLQDFRGRVPILHLRDYKLKPMGLIFGGGTDCEAGKGNLPFRQILQQAKSAGVE
                     LGVIEQSTKTPIESIRSSAEHLFSLLNE"
     gene            complement(3836..4909)
                     /locus_tag="PLD98_06380"
     CDS             complement(3836..4909)
                     /locus_tag="PLD98_06380"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HQA05906.1"
                     /translation="MNSYINKVFELADASVKRFPPHKLNWSWGQALYLYALSKIDTEF
                     NQERYTAYIKKYYDHHIKKGYRVNTSDTSAPALAAYYLAQKEGGEPYRAVVERVRKYF
                     DEVPKVVEDMPNHLGNGPESLIYPKSIWVDSIMMYGVFTNWYAYTEGDEKLREFASRQ
                     PRMFSKYLFDEEDNLFYHSYWTNFKTHYPRQKIFWGRGNGWVMAAIPLFMDYLPEGEE
                     KEYTKELFKRLAASLLKYQKENGYFATVINRPDETYEESSATMLIASGWFYGWRTGLL
                     GDEYYHAAKKAFHAVVEDFEYRDGLLSMPKISAPTSAIQGIPYLVYRITPRGNDWHYG
                     LAASFFAALEYKRCLEKEQAALK"
     gene            complement(4906..6858)
                     /locus_tag="PLD98_06385"
     CDS             complement(4906..6858)
                     /locus_tag="PLD98_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05907.1"
                     /translation="MLLYLENPALQKERGFELNNENIIAALKPAKKYIDGRYDCQDFI
                     MQSLLRIQYSHIDTIRLISPEGAQMIEDIFLNAKYWMTEPGEDSMCYWSENHQILYGV
                     AEYLAGQMWKDRIFTNDGADGKEHMERGRRRINYWMEQRFLYGFSEYNSTNYYLFDVG
                     PAANFIEFAAPEDADMVERMKMCLDLLLFDVASNMHKFTFTAPTGRAYANNMVGITGD
                     SVSRLIDFLWQRNDNYKTDAHKMLLNFYSMYFAKHPDGTPKKLYEFPRVLYEIGLDEE
                     ERVIKSSHSMDTWELEEKGYVGHSDKQIMRQLSMEAFTNPEVIYNTVSYFDKHNLFSN
                     SFVNYFKAINLKLLRHPDRLRFISEKLNPMPNGIALQRANLYCYQTKHYQLMNVQRYH
                     PGIYGATQMLQVLNFGGKSVVFTTHPAKNEDKKSASAYPGYWAGFGRSPHAVQHKNVL
                     LMLYKIPKTPGFLELYSVPQFSHTYLPEAFLDEVEINGRYAFVRHGKAFLAIIGKNEL
                     HYKPFSMYSAKALKNGLKDYPDKRFDLIQEGNYHYWIYELSDEDKESFEEFKSRIMEN
                     SIEYDGSSKLVYKSQNHEYKTVYGGAFFVDGEEQPLEYERFESDYCHAKREADEFFFS
                     FKGHYLRLNFDKAIREYGEASGGSEQ"
     gene            7305..8135
                     /gene="kduI"
                     /locus_tag="PLD98_06390"
     CDS             7305..8135
                     /gene="kduI"
                     /locus_tag="PLD98_06390"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HQA05908.1"
                     /translation="MDIRYSVSKNEFKSMDTAALRKNFLIDDLFQDDKIKLCYSHYDR
                     IIAGGAVPKAKELVLEASDELAAKYFLERREMGIINIAGDGSVVADGISYPMKKYDCL
                     YLGRGTKEIKFISDSAEKPAVFYFNSTPAHKEYPTVHIPKEKAVQAALGSSTDCNERI
                     IYKYILPGNVESCQLVMGLTILAEGSVWNTMPTHTHERRMEVYLYFDVPKNHTVFHMM
                     GPGDETRHITVRDRQAVISPSWSIHSGVGTKNYSFIWGMCGENQDFDDMQGIATDDLL
                     "
     gene            8199..>8459
                     /locus_tag="PLD98_06395"
     CDS             8199..>8459
                     /locus_tag="PLD98_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQA05909.1"
                     /translation="MILDKFKLDGKIAVVTGANTGLGQGICIALAEAGATVVGVARRS
                     CEETAAQIEKGRFFELRADLSDMNNAEYVIDETLKQFGKIDIL"
BASE COUNT         2258 a         1840 c         1954 g         2407 t
ORIGIN      
        1 ctctaattgc ccttttaaaa cgggcataaa taattttcaa catttttcgt ggaaatattg
       61 accacctgat gcaaatatga tatcatgttg atatcaatta catatcggag gctaatatga
      121 agaacacaga gctttttaca gagcagaaga taattaaagg caagaacgga cttgccgctt
      181 tgttcctgat tttgcttgcc cttttagcct ttaccgccct tggaattgcg ggcatagtca
      241 tcgccagcag agcagcgcga tatggcagca taataatgcc ttacagtgga cccagtgaga
      301 atttcggcgg cgcgctgatt gtcatttcga ttatcggttt tgtggcttct acaattatgt
      361 tcgcaggtct taaaacttta aagcccaacg aggctttggt tctgaccctt ttcggcaaat
      421 acttcggcac cttggagggt ccgggattct tctttgtaaa cccctttgtc actgcggtga
      481 accccataaa gcgtacgccg atagccgtta acaccgcggg aacggtggat ctccagccca
      541 aacccgctca aagaggcgaa aaaatggacc ttatcgattc tgcaagcggc aaggcaatat
      601 cgcttaaaat aatgactctg gacaacaaca agcagaaaat caacgacaag ctgggcaacc
      661 caatagaaat cggcgtcgtt atagtttggc gtgttgtcaa tgcggcaaaa gccgtattta
      721 atgtggataa ttactttact tacctttcca cgcaggcgga cagctcgctg agaaatatcg
      781 taaggctctt cccctatgat gtatggcagg acggagagga agccgacgaa atgtcattaa
      841 ggggcagcag tcaggaggtc gctgtgaagc tccgcgaaga actgcagtca aaggtggaaa
      901 tcgcgggtct tgaaattgtg gaagctcgca ttacccacct tgcctacgct cccgaaatcg
      961 ccgccgccat gctccagagg cagcaggcaa gcgccattat cgatgccaga aagctcattg
     1021 ttgagggtgc ggttggcatg gttgagatgg ctctgcagaa gctcagcgag cagaacatcg
     1081 tagagcttga cgcagaaagg aaggccgcca tggtttccaa cctcctggtt gtcctttgcg
     1141 gaaataagga agcccagccc atagtcaaca gcggttcgat ttactaaagg ttatgagcag
     1201 caataaaaag caaatccccc ttcgcctgtc gcctgagctt tatgaaaaat tagccgcatg
     1261 ggcagaggat gaattccgct ccctcaacgg tcagattgaa tatctcctca ccgagtgcgt
     1321 aaaaaaacgc tacggcgaga aaaaagacaa ataaaaaacg gaggctaata gcctccgtat
     1381 attttttaat ctttgttctc gaatttgttt gcatacagcg ttttgaaata tcatgggcaa
     1441 taaatatttc cgtagaagat taataatttg gctcactttt agggggttat acttctgtca
     1501 aaacttgctc tttgttttcc cgctcatagg cgttcatttt ttcaagacgc atttttgcga
     1561 gttcttcata cattcttctg cgcttgtcgc cggagaggtc atagaaaagt tttggaataa
     1621 tgcccagcag accagttgcg gcgggcataa gcatgctcat ggcaaagagg aaatacttcg
     1681 ttttttcgga ctgctttcca aagcctgcgt ccaaggaata gcctgctttt ttcataattg
     1741 cggaattgaa ggaatttgtg acattgcgga acaatttata cgccatgctt ttggtggcaa
     1801 gggtcactcc ctcggttctg tatccgaatt tccattcgca gtagtctaag gcgtcggtca
     1861 gaatttccct cgggagaatt ttatttatag acaatgtgcc tttatagaaa atatcccgca
     1921 ccattaaaag cacagtcatt attagtctgt tctgatatat tcctttaggg ccgataccac
     1981 gcattgagcc tattacgaaa attcccaatg ttatgacatc tttaagatgc tgaccgaata
     2041 tccaaagcga tttggttgaa aagcgtgcgc gtacctgatt gatatatgta taacttaaaa
     2101 tggagagggg agcgccggga attataatca cattgcgcaa agagctggag cccagcacat
     2161 cgataaaata gttgtgttcc caaacgccga cagggaattt ggatatcaaa tcgctgacaa
     2221 gaataattag catgggcttg ttgtttatta ttgttctgaa tccctcttta tagttgtggc
     2281 gttctcggga ttgggcgata cgctctgtgg aatagatgga aaagaacaag gcaagaatgc
     2341 ctcccgaaat agtgcaaaag gttcccatgc cgataaaggc gttgtccata gtcattttga
     2401 ttttcttgtg gtttacaagg tcaattaacg ctcccatgat aatttgggga aaggactccc
     2461 aaagtccgga gacgacctcg gcagtggtat aaaggcgcac tctgtcttca gggtttgggg
     2521 ttatatttgc caaaagaccc gtttccgaga tttctcgcaa ggttccaact gcttcccttg
     2581 ccattgcgag aatgagccag aaagcaattt ttctcatatc taaggggttt ttgtcgaaga
     2641 acaggggaga aagccagtaa agacaggtaa gggcggttcc cggcaaagca tacgccagaa
     2701 ggtaggggcg gaacttgccc cagcgggttt ttgttttatc gattaacgag ccaagtatgg
     2761 agtcgttaat tacatcccaa atgccgttta taaaattgat tattgtgttc caatacaaat
     2821 caattttcag aacatcaata ataaagcggg tttggtattc atcgatgtta aagctttttg
     2881 atgagtcaaa aagcacatag gctattgttt cttttctgtt tacatagcgc cgtgtgtagg
     2941 tgtcttttgc cagcgtttgg atatccatgt cctaccccca aaactcagtg ttaagctccg
     3001 cctcattcat tcagcaagga aaaaagatgt tccgcgcttg accttatgct ttcaatgggc
     3061 gttttcgtgc tttgttcaat cacgccaagc tccacacctg ccgactttgc ctgctgcaat
     3121 atttgcctaa aggggagatt gcctttgcct gcctcacagt ctgtgccgcc gccgaaaata
     3181 agtcccatgg gcttgagttt atagtcacgc agatgaagaa taggcacacg gccccggaaa
     3241 tcctgaagca aagtgagaat attgccgccg cccttttggc accaatatgt gtcgacagta
     3301 aaaagacccg aaaagttttc ccgcataagc tccagtacgg tctttccgtc aaactttaga
     3361 aattcgtaat gatggttgtg gtgagaaaga agtatatcct gccgctttag tttttcatat
     3421 aggctgttca gctgttcaca atagcgcaga acgccctcct tgccctttct cagcgcggag
     3481 aggtcaatca cgctgtttga tataaatctc gcgccgagaa tatgcatgaa ttcagcggtt
     3541 aagcccgtgt ctttcgcggc ggtattgtaa cgaatctgac aggaaaccgt ttctataccg
     3601 tgagcttttg ccgcatttgc aataagctct gccgtctttg cggtaaacgg catttttaaa
     3661 taatccacgg caagctcaat atgttttaca ccgaagcctg agattaaaga gaaggtttta
     3721 tctatttcct tctcgttttt cataacggaa cgacatgtaa aggtttgcag accaatcttc
     3781 atcgcatttg cggcttatgc cgccccgctc ctttcaaaca caatttagtt tagatttact
     3841 taagagctgc ctgttctttt tcaaggcatc ttttgtattc caaggcagcg aaaaagcttg
     3901 ccgcaagtcc gtagtgccag tcgtttcctc tgggagtaat gcggtataca agataaggaa
     3961 taccctgaat cgcgcttgtc ggtgcgctga ttttcggcat gcttaaaaga ccgtcacgat
     4021 attcaaaatc ctccacaacc gcatggaaag ccttttttgc ggcatggtag tattcatcgc
     4081 ccaaaagacc tgtgcgccag ccgtaaaacc agccgctcgc tatgagcatg gtggctgagc
     4141 tttcttcata tgtttcgtcg ggacggttaa taacagtcgc gaaataaccg ttttctttct
     4201 gatatttaag caaagacgcg gcaaggcgct tgaaaagctc ctttgtatat tccttttctt
     4261 ctccctctgg caaataatcc ataaagaggg gaatcgcagc cataacccag ccgttgccgc
     4321 gtccccaaaa gattttttgc cgcggatagt gggtcttaaa attcgtccaa tagctgtgat
     4381 aaaagaggtt atcttcctcg tcaaagagat acttgctgaa cattcgaggc tggcgggagg
     4441 caaactccct aagtttttcg tcgccctccg tataggcata ccagttcgta aagaccccgt
     4501 acatcatgat gctgtctacc cagatgcttt tcgggtaaat aagactttcg gggccgtttc
     4561 ctaagtggtt gggcatatct tcaaccacct tcggaacctc atcaaaatat ttgcgcaccc
     4621 tctcaacaac agccctgtaa ggttcgccac cctctttttg agcaaggtaa taggcggcga
     4681 gagccggagc ggaagtgtcc gaagtgttga ccctgtatcc cttttttatg tggtggtcat
     4741 agtacttttt tatgtatgcg gtgtagcgtt cctgattaaa ttcggtgtca attttggaaa
     4801 gggcataaag atagagtgcc tgaccccatg accaattcag tttatggggc ggaaagcgtt
     4861 taacagaggc gtctgccaat tcaaatacct tgtttatgta tgagttcatt gttcactacc
     4921 tccgcttgcc tcgccatatt cccgaatagc tttatcgaag ttcagacgca agtaatgacc
     4981 cttgaatgag aagaagaatt catcggcttc acgctttgcg tggcagtaat cgctttcaaa
     5041 acgctcatat tcaagaggct gttcttcccc atccacgaaa aacgcgccgc cgtagacggt
     5101 tttatattca tggttttggc ttttgtaaac gagtttgctg ctgccgtcgt attcaatgct
     5161 gttttccatg attcgacttt tgaattcttc aaagctttcc ttatcttcat cggaaagctc
     5221 ataaatccag taatgataat tgccttcttg tattaaatca aaacgcttat cgggatagtc
     5281 tttaagtccg tttttcaaag cctttgcgct gtacatactg aagggcttat agtgaagctc
     5341 attcttgcca atgatggcaa ggaacgcttt gccatggcgc acaaaggcgt atctgccgtt
     5401 tatttcaact tcgtcaagaa acgcttcggg caggtatgta tgactgaact gaggaactga
     5461 ataaagctcc aaaaagcccg gtgtctttgg aatcttgtaa agcataagca gcacattttt
     5521 atgctgcacc gcatgcgggg agcgtccaaa gcccgcccag tagcccggat aagccgaggc
     5581 gctttttttg tcttcgtttt tggcggggtg ggttgtgaat actaccgatt ttccgccaaa
     5641 gttcaaaacc tgcagcattt gtgtggcgcc gtatatgccg gggtgatatc tctgcacatt
     5701 cattaactga tagtgcttgg tctgatagca gtataagttt gccctttgca gagcgatgcc
     5761 gttgggcatg ggattcagct tttcgctgat aaaacgaagt ctgtcagggt gacggagaag
     5821 tttcagatta atggctttga aatagtttac aaagctgttg gagaacaaat tatgtttatc
     5881 aaagtagctt acggtattgt atatgacctc aggatttgtg aacgcctcca tgctgagctg
     5941 gcgcattatc tgtttgtcgc tgtgacccac atatcccttt tcttcaagtt cccatgtgtc
     6001 catagagtga gaggatttta taactctttc ttcttcatca agaccgattt cataaagtac
     6061 tctggggaac tcataaagct ttttcggcgt tccgtcgggg tgctttgcga aatacatgga
     6121 atagaaatta aggagcattt tatgggcgtc ggttttgtag ttatcgtttc tttgccatag
     6181 gaaatcaatg agcctgctca ccgagtctcc cgttatgcct accatattgt tggcataagc
     6241 gcggcctgtg ggagccgtaa atgtaaactt gtgcatattg gaggcaacat caaaaagaag
     6301 caaatccagg cacattttca tgcgctcgac catgtccgcg tcttcgggag cggcgaactc
     6361 aataaagttt gcggcgggac ccacatcaaa aagatagtag tttgtggagt tgtattcgga
     6421 gaaaccgtaa agaaaacgct gctccatcca gtaatttatt cttctgcgtc cgcgctccat
     6481 atgctccttg ccgtcagcgc cgtcgtttgt gaatatcctg tctttccaca tttgacctgc
     6541 cagatattcg gcaacgccgt aaagaatctg atggttttcg gaccagtagc acatggagtc
     6601 ttcccccggt tcggtcatcc aatatttagc gttgaggaaa atgtcctcaa tcatttgcgc
     6661 tccttcgggg gagattaagc gaatggtgtc aatatgggag tactggatac gcaaaaggct
     6721 ttgcattata aaatcctggc agtcatagcg tccgtctatg tatttttttg cgggtttaag
     6781 agccgcgatt atattttcat tgtttagctc aaaaccgcgc tctttttgca gggcgggatt
     6841 ttcaagataa aggagcaatt cgtgtattag ctccgaccct tcattgcttt tgggatgatg
     6901 cggcaatggg cggattgata aattattaaa tatgtctatg gcaaaatacg ccccaacagc
     6961 ggctgccaga attgttgcgg cgattttcga ttttgatgtc ttattaataa aaatcactcc
     7021 tttatataaa ctttaggaat tttgcttttt atgttttctc attgtctgag gaaagtggct
     7081 gaattgtata tttttataaa aaatgcaatt taattgtaag ttattctatg aaagattgtc
     7141 aaatgttttt tggaatttag aaaaatactc cagcagcctt ttcctttgca attgacagcc
     7201 tttgaaatat tgatataatt aaagttgagt tttggtaaaa ttttgtaatg tttattagta
     7261 agaaaaggtt tgcctgccgg tgcataaaaa aggagagttt gaaaatggat atacgctaca
     7321 gcgtttcaaa aaacgaattt aagagcatgg acactgccgc gctgcgcaaa aatttcctga
     7381 ttgacgactt attccaagat gacaaaatta aactttgcta ttcccattat gaccgaatta
     7441 tcgcgggcgg agcagtgcct aaggcaaagg aacttgtact ggaagcctcg gacgaacttg
     7501 ccgcaaagta cttccttgaa agaagggaaa tgggcatcat caacatagct ggcgacggtt
     7561 cggttgtagc cgacggcata tcttacccga tgaaaaaata tgactgcctt tatctgggac
     7621 gcggaactaa ggaaataaag tttatttccg attctgccga aaaacccgca gtgttctatt
     7681 tcaattccac ccccgctcac aaagaatatc ccacggtaca tattcctaag gaaaaggctg
     7741 ttcaggcggc tttaggctca tcaaccgact gcaacgagcg catcatatac aaatacattc
     7801 tgcccggcaa tgtggagagc tgtcagcttg ttatgggtct tacgattctg gctgaaggca
     7861 gtgtttggaa caccatgccc acacataccc atgagcgcag aatggaggtt tatctctatt
     7921 ttgatgtgcc caaaaaccat acggtatttc atatgatggg ccccggagat gaaacccgtc
     7981 atataaccgt cagagacagg caggcagtga tttcgcccag ctggtcaatt cacagcggag
     8041 tcggcacaaa gaactattcc tttatctggg gtatgtgcgg cgaaaatcaa gactttgatg
     8101 atatgcaagg catcgcaacg gacgacttgc tgtaaaatta aaatccgcgc agcccttttg
     8161 cggggctttg cggatttttt cataaaggga ggtaaatcat gattttagat aagtttaagt
     8221 tggacggaaa aatcgctgtt gttacaggag ccaacacagg cttgggacaa ggcatctgca
     8281 tagcccttgc ggaggctgga gcaaccgttg tcggcgtcgc gagaagaagc tgcgaagaaa
     8341 cggcggcgca gattgaaaag ggccgctttt ttgaactgcg cgcggatttg tccgatatga
     8401 ataacgcaga atatgtcata gatgaaacct taaaacaatt cggtaaaata gatattttg
//
LOCUS       DAONVQ010000087         8294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_6547_length_8294_cov_4.512926, whole genome shotgun sequence.
ACCESSION   DAONVQ010000087 DAONVQ010000000
VERSION     DAONVQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6547_length_8294_cov_4.512926"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLD98_06400"
     CDS             <1..202
                     /locus_tag="PLD98_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009534250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQA05910.1"
                     /translation="DKGKRLKIFYITQASTRPPTFVCFVNNKELFHFSYQRYLENEIR
                     SVFGLEGTPVRFLIRERERDSD"
     gene            204..872
                     /locus_tag="PLD98_06405"
     CDS             204..872
                     /locus_tag="PLD98_06405"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HQA05911.1"
                     /translation="MTDSMDKKCIIVGAGDFTPDFKKPSAGDIVIAADGGLLHLQSLN
                     IRPDILIGDFDSLNKKVDSSLSDEIIRLKIDKDYTDTYAAAKIGLERGFKTFELYACT
                     GGARFDHTLSNIQLLAFLIEQGCDAYIAAPDFMLTGVKNGKLSFGKEAEGYVSVFSHT
                     DISSGVTLKGLKYPLNNATINSRFPIGVSNRFMGTESSIEVKDGILIVSHCLGFRYSS
                     KESF"
     gene            955..1806
                     /locus_tag="PLD98_06410"
     CDS             955..1806
                     /locus_tag="PLD98_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HQA05912.1"
                     /translation="MANFPIGVIVDSFRIDIKEALKKAAAMGVQGVQVYSTRGAMAPE
                     NLSPAQRREFLDYVKSLGLKISALCGDLGGGGFVHPDKNPEKIEKSKRILDLAKDLET
                     DVVTTHIGVVPADPNHDRYKILQEACHELAEYADSLEAHFAIETGPETSAVLKQFLDS
                     LNSKGVAVNLDPANLVMVTGDDPVKAVHTLKDYIVHTHAKDGVQLFYKDPELVYGMVK
                     SEIVTGPSFEEVPLGTGSVDFPNYLKALKDIGYTGFLTIEREVGDNPERDIQIAVDFL
                     RETMAKI"
     gene            1830..3209
                     /gene="argH"
                     /locus_tag="PLD98_06415"
     CDS             1830..3209
                     /gene="argH"
                     /locus_tag="PLD98_06415"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQA05913.1"
                     /translation="MKLWAGRFSKEADKVTDAINFSIGIDKRMWREDIEGSIAHSKML
                     SERGIISNEDKERIVAALRQLSDEIEKGETVISEEYEDIHSFVEAVLTQRIGDAGKRL
                     HTARSRNDQVAVDIRLNLRNETDRLLKLLEELIALLSDMAETYADVIMPGYTHLQRAQ
                     PITFGHHLLAYAEMLLRDRGRLIDAKKRMNVCPLGAGALAGTTYPIDREMTAKELGFD
                     APARNSLDAVSDRDFCIELASALSIIMVHLSRFSEEIILWCSHEFKFIELDDAFSTGS
                     SIMPQKKNPDIAEIVRGKAGRVFGSLMTLLTVMKGLPLAYNKDMQEDKEAIFDAFDTV
                     SLCLMAFTPMLKTIKVNKEEMRKAAAGGFINATDCADYLVGKGLPFRDAYKITGSLVA
                     YCIENSKVLEDLSLEELKEFSPLFEEDVFKAISLETCVNNRTSYGGPAPENVRLQAKA
                     VKSELSGSK"
     gene            3230..4108
                     /locus_tag="PLD98_06420"
     CDS             3230..4108
                     /locus_tag="PLD98_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HQA05914.1"
                     /translation="MKICVSSYSFSSLMKSGEYTLLDCISLAKEIGCEGIEIAGINPP
                     DGVTKEEYAAALRDEAKKQGMEMVNYTIGADFLNGSDGDFDAEVERLKKEVDIAAILG
                     VSGMRHDATRGYDAANRHQRGFDDALPTLIEGCRRVTEYAATKGIRTMVENHGYFCQD
                     SRRVEKLVNGVGHDNFGILIDIGNFLCADEAPEIAVARLAPYAYHVHAKDFHVKSGNA
                     LPPQNGFFKTRAGNYLRGAIIGHGEVPVLQCMSILKNAGYDGYVSIEFEGIENAKLGV
                     QYGFNTLKYIKDFLNW"
     gene            4253..5116
                     /locus_tag="PLD98_06425"
     CDS             4253..5116
                     /locus_tag="PLD98_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05915.1"
                     /translation="MKRKLIALLLILSVLLGFAACGKKATDETTTAAPEETTAAEESS
                     ETAAETEQNTDASETQATSEAPSQGNNPTEAPSSPSGDVISNSSTKQQIADYYNKATA
                     ATKKANKLRGKESVELSKISVNNNDSGAIISFIKGILGPVIESNYEVSDSKPIPYHDQ
                     EVNVLADDIKSFSVTDNGDTYTLKIMPKDDINPKKSLEGSTGRFFPIVENIKEIAAGV
                     PGVKFEGGVEQGVTADYKDAVIEVKIDKKTYEIVGGTYSMNIYLNINKASASIFKVDK
                     ATAIISYKLVL"
     gene            complement(5159..5692)
                     /locus_tag="PLD98_06430"
     CDS             complement(5159..5692)
                     /locus_tag="PLD98_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HQA05916.1"
                     /translation="MGKIFKKKQLVLTALVVLLGVAVYINWYYTRPENQVDNNLTEST
                     TQTGNLGEAEFVDGQTPEGEEYFAAVKLNRSTAHDEALETLKLLIKDDATPEEVAAAA
                     DTIEKFSNRLKSESDIELLVKAKTGSECVAVINEDRIDIVVSNSVLNDNTVLQIKEIV
                     MNNSDIPPENITITGVK"
     gene            complement(5707..6246)
                     /locus_tag="PLD98_06435"
     CDS             complement(5707..6246)
                     /locus_tag="PLD98_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05917.1"
                     /translation="MSKLFENLKKDKKALWIMLIGVAVMLIIMGTEFVPGGKSKKKHS
                     DAESPSIGDYESILEDKLTDLLASVSGAGRTKVMVTLDRTEEYVYIYDESKEEEIKGD
                     GGTAKSSTKTSYIIIEDGKSSGESGLKSVIIMPKVKGVAVVCEGGDIPSVKQQINEIV
                     TSVFDIPSTKVSIAKMKQK"
     gene            complement(6287..6763)
                     /locus_tag="PLD98_06440"
     CDS             complement(6287..6763)
                     /locus_tag="PLD98_06440"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HQA05918.1"
                     /translation="MEGIKEWAVNLAICFIASSIVVYLIPKGKLEKPMNTLLSLFLLS
                     VILMPFIEKSFDLDDLPKRREDFGIDLEDKAETVRDEMNYLMEKQGKAAVEILVGEKL
                     KSLGLNYSEIEVITDIAEDSRIIIKDIVIKAELTEPQKQQAAEEIEKLTGIRPKFE"
     gene            complement(6776..7930)
                     /locus_tag="PLD98_06445"
     CDS             complement(6776..7930)
                     /locus_tag="PLD98_06445"
                     /inference="COORDINATES: protein motif:HMM:NF021088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05919.1"
                     /translation="MKNKVKKILCGIIISLFLLPLLGAQAHAESSEIDESEVYSEQLK
                     SAGAYELFNLIDDDTREMLKELGIEDFDAQHIFDLSPRKIIDLIFSIVTGGLKEPLKA
                     AFSLVGIVILFSLISCYIPDLEGKGSIFGYVFVLAGAMIIIKPISQCIGGAVSAINAT
                     SKVMLTLVPILTGLIAAAGKAASALSYNGIAFTAAQVSSQVCNSFITPFSGVYMALSM
                     AGSINPIINIESIANFIKKATTIVLSFAGTIFAGLLTIRGILAGSVDALALKGAKFVI
                     GTAIPVIGSSIGDALSSIIGSLQVVKSTVGAVGILIVALINIPAVIHILLWIISLKGA
                     AFASEMFGIESIQKMIDGLASGLSVIFAILCFNAVLMIVSLGLVVSISNG"
     gene            complement(7941..>8294)
                     /locus_tag="PLD98_06450"
     CDS             complement(7941..>8294)
                     /locus_tag="PLD98_06450"
                     /inference="COORDINATES: protein motif:HMM:NF018401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HQA05920.1"
                     /translation="ISVLYVLLWVHKREFTILLQLGAVVVILLLILPDLKQLLDTFQN
                     LIDISSVKTEYVAILAKALGISLITELAHDTCIDCGESALANKVELAGKTVVLTLSLP
                     LLISVARFALELIGK"
BASE COUNT         2237 a         1995 c         1924 g         2138 t
ORIGIN      
        1 cgacaagggt aagaggctca agattttcta cattactcaa gcctccacaa gaccgcccac
       61 attcgtttgc tttgtaaaca acaaggagct tttccacttc agctatcaaa ggtatcttga
      121 gaatgaaatc cgctctgttt tcggacttga gggcactccc gtcaggttcc tcataagaga
      181 gcgtgaaagg gacagcgatt aatgtgactg atagcatgga taaaaagtgc ataattgttg
      241 gagcgggtga ttttacgcct gattttaaaa agccttccgc cggcgacatt gtaattgccg
      301 ccgacggcgg gcttttgcat ttgcaaagcc tgaatattcg acccgatatt ctgattggcg
      361 actttgattc cttaaacaaa aaggtggact catctttgtc cgatgagatt attaggctta
      421 aaatcgacaa ggactatacg gacacctatg ccgccgcgaa aatcgggctt gaaagaggtt
      481 tcaagacctt tgagctctac gcctgcactg gcggagcaag gtttgaccac actctttcaa
      541 atattcagct cctcgcattt ttgattgagc agggctgcga cgcatacata gcagctcccg
      601 attttatgct gaccggcgtt aaaaacggaa agctcagctt tggaaaagaa gctgaaggct
      661 atgtttcggt tttctcacat actgatatat caagcggcgt tacattaaaa gggctaaagt
      721 atccgcttaa caacgctacg ataaattcaa ggttccccat aggcgtcagc aaccgcttta
      781 tgggaactga aagcagcatt gaagtaaaag acggcatttt gattgtttcg cactgcctcg
      841 gctttaggta tagctccaaa gaatcctttt aataagggct tttattattg actgcgtaca
      901 gccgcagagt atataattta accaatatcc gaatacatat atggagtgat tttaatggca
      961 aacttcccta tcggcgttat tgttgacagc ttccgcatcg acatcaaaga ggctcttaaa
     1021 aaggctgccg ctatgggtgt tcaaggcgtt caggtttatt ccacaagagg agcaatggct
     1081 cccgagaatt taagccccgc tcaaaggcgt gagtttttgg attatgtaaa atccctcgga
     1141 ctcaaaatat ccgctctctg cggcgacctt ggcggcggcg gatttgttca tcccgataag
     1201 aaccctgaaa agatagagaa atctaagaga atccttgacc ttgccaagga ccttgaaacc
     1261 gatgttgtta caacccacat cggcgtggtt cccgctgacc cgaatcacga cagatataag
     1321 attcttcagg aggcttgcca tgagcttgca gagtatgccg actcactgga agctcacttt
     1381 gcaatcgaaa ccggtcccga aacctccgca gttttaaagc agttccttga ttcactcaat
     1441 tcaaagggcg ttgcagtaaa ccttgacccc gcaaaccttg taatggtaac aggcgacgac
     1501 cccgttaagg ctgttcacac cctcaaagat tacatagttc acacccatgc aaaagacggc
     1561 gttcagctgt tctataagga ccccgaactt gtctacggca tggttaagag cgaaatcgta
     1621 acaggcccgt catttgagga agttcccctc ggaacgggca gcgttgactt ccccaattac
     1681 ttaaaggcac tcaaagacat cggctacaca ggcttcctca ccattgagcg cgaagtcggc
     1741 gacaaccccg aaagagatat tcagattgct gttgacttcc tgagagaaac aatggcaaag
     1801 atataaatta cttgaaaggc agaaatgtaa tgaagctctg ggccggaaga ttttcaaagg
     1861 aagcggataa ggtcaccgat gccataaact tctctatcgg cattgacaag agaatgtggc
     1921 gtgaggacat tgaaggcagc atcgctcaca gcaaaatgct atctgagcgg ggcattatct
     1981 ccaatgagga taaagaaaga atagtcgctg ccctgcgtca gctttccgat gagatagaaa
     2041 aaggcgaaac agttataagc gaggaatatg aggatattca ttccttcgtg gaggctgtgt
     2101 tgactcagcg cataggtgac gcgggcaaga gactccacac cgcaaggagc agaaacgacc
     2161 aggttgcggt ggatatcagg cttaacctca ggaatgaaac agacaggctg ctgaaacttc
     2221 ttgaggagct tatcgccctg ctctccgaca tggcggaaac ctatgcggat gtaataatgc
     2281 ccggctatac tcatctgcaa agagctcagc cgattacctt cgggcaccat ctgctggcat
     2341 acgctgagat gctgcttcgt gacagaggaa gactcattga cgccaaaaag agaatgaatg
     2401 tctgccccct cggagcgggc gctcttgcgg gtaccacata ccccattgac agggagatga
     2461 ccgcaaagga gctgggcttt gacgctcccg caagaaactc ccttgacgcc gtatccgaca
     2521 gggatttctg catagagcta gcttctgctt tgtctattat catggttcac ctctcacgct
     2581 tctctgagga gattatcctt tggtgttccc atgagtttaa gttcatagag cttgacgacg
     2641 cgttctctac cggctcaagc attatgcccc aaaagaaaaa ccccgacatc gcggagattg
     2701 tcagaggcaa ggcgggcagg gttttcggca gtctaatgac tctcctcacc gttatgaagg
     2761 gtctgccgct tgcttacaac aaggatatgc aggaggacaa ggaggccatc tttgacgcct
     2821 ttgatacggt aagtctgtgt cttatggcct ttaccccgat gctcaaaacc ataaaggtaa
     2881 acaaagagga aatgagaaaa gcggccgcgg gcggattcat caacgccacc gactgcgccg
     2941 actaccttgt gggcaagggc ctccccttcc gcgacgccta taaaataaca ggctctttgg
     3001 ttgcttactg catcgaaaac tcaaaggttc ttgaggattt aagccttgag gagcttaaag
     3061 agttctcccc tctctttgag gaggatgttt tcaaagcaat cagccttgaa acctgcgtaa
     3121 acaacaggac atcctacggc ggacctgctc ccgaaaatgt aagactgcag gcgaaagccg
     3181 tcaaatccga actcagcggt tccaaataaa gcgcgaaagg atttttaata tgaagatttg
     3241 cgttagctcc tacagctttt catcgctaat gaaaagcgga gaatacactc tgcttgactg
     3301 catatccctt gcaaaagaaa tcggctgcga gggaattgag attgcgggca ttaatccccc
     3361 cgatggagtt acaaaggagg agtatgccgc agccctcaga gatgaggcta aaaagcaggg
     3421 catggaaatg gtaaactaca ctatcggcgc cgacttttta aacggcagcg acggtgactt
     3481 tgacgccgag gtggaaaggc ttaaaaaaga agtagacata gccgctattt tgggtgtttc
     3541 gggcatgcgc cacgacgcca caagaggcta tgacgcagca aaccgccatc aaagaggctt
     3601 tgacgacgct ctgcccacac ttattgaagg ctgcaggcgc gtaaccgagt atgccgcaac
     3661 aaagggcatt cgcaccatgg tggaaaacca cggctacttc tgccaggaca gcagaagggt
     3721 tgaaaagctg gtaaacggcg taggtcacga caatttcggc atacttattg atataggcaa
     3781 cttcctttgc gccgacgagg ctcccgagat tgccgtcgct aggctggctc cgtatgccta
     3841 tcatgttcac gcgaaggact tccatgtaaa gagcggcaac gctttgccgc cgcagaacgg
     3901 ctttttcaaa acaagagccg gcaattacct cagaggcgca ataatcggcc acggcgaagt
     3961 tcccgttctt cagtgcatgt ccattcttaa aaacgcaggc tacgacggct atgtttcaat
     4021 agaatttgaa ggcattgaga acgcgaagct gggagttcag tacggcttta atacgcttaa
     4081 atacataaag gatttcctaa actggtaaat ttaaattgcc gacagcactt tgcttgtcgg
     4141 caattattta tttgcattga ttaagtttat aagaaatact gtacaaaaat ttcaaaatgt
     4201 gatattatgc cattgtgcac attattgtgt tatgttctta ggaggaaata aaatgaaaag
     4261 gaaactgata gcacttctac ttattctgtc tgttctttta ggctttgcgg catgcggaaa
     4321 aaaggcaaca gatgaaacaa ccacagcggc tcccgaggag accacagccg ctgaggaatc
     4381 gagcgaaacc gctgccgaaa ccgagcagaa cacagacgca agcgaaactc aggccacaag
     4441 cgaggctccg tcccagggca acaaccccac tgaggctccc agctctccca gcggcgatgt
     4501 aatcagcaat tccagcacaa agcagcagat tgccgactac tacaacaagg caaccgccgc
     4561 aacaaagaag gctaataagc tgcgcggcaa ggaaagcgtt gagctttcaa agatttctgt
     4621 taacaacaac gacagcggcg ccattattag cttcattaag ggcatccttg gtcccgtaat
     4681 tgagagcaat tacgaagtca gcgacagcaa gcccattccc tatcatgatc aggaagtaaa
     4741 tgtgcttgcc gatgacatta agagcttctc cgtcaccgat aacggcgaca cctatactct
     4801 taaaataatg cccaaggatg acatcaaccc taagaagagc cttgagggtt ctacaggcag
     4861 attcttcccc attgttgaaa acattaagga aattgccgca ggcgttcccg gagttaagtt
     4921 tgaaggcggc gttgagcagg gcgtaaccgc cgactataaa gacgctgtta ttgaagtaaa
     4981 gattgataag aaaacctatg aaatcgtagg cggcacctac tcaatgaata tttaccttaa
     5041 cattaacaag gcaagcgcct caatattcaa ggtagacaag gcaaccgcaa ttatttccta
     5101 caagctcgtt ctgtaagtta agaaaagatt taagcgacag gctaagcctg tcgctttttt
     5161 atttgacgcc ggttatggtt atgttctcgg gcggtatgtc gctgttgttc atcacgattt
     5221 ctttgatttg cagcactgtg ttgtcattga gaacagagtt ggaaaccaca atgtcgattc
     5281 tgtcctcgtt gatgactgcc acacattcgc tgcccgtttt tgccttgaca aggagctcta
     5341 tgtcggattc gctcttgagt ctgttggaga atttctcaat tgtatcagcg gctgcggcta
     5401 cttcttcggg tgtagcgtcg tccttaataa ggagcttgag ggtttcgagc gcctcatcat
     5461 gggctgtgct gcggttgagc tttactgcgg caaagtactc ctcgccctcg ggggtctgac
     5521 cgtccacaaa ttcagcttcg cccaaattgc ctgtctgggt ggttgattcg gttaagttgt
     5581 tatcgacttg gttttcgggt cttgtatagt accagttgat atataccgct acgcctaaaa
     5641 gtaccactaa tgctgtaagt accagttgtt ttttcttaaa aatcttgccc atagtttgcc
     5701 tccaatctat ttctgtttca tttttgctat tgataccttg gttgaaggta tgtcaaagac
     5761 agatgtgaca atctcgttta tctgctgctt tacactcgga atgtcgcctc cttcacatac
     5821 aaccgccact cctttaacct ttggcatgat tattacgctt ttaagtccgc tttcgccgga
     5881 tgacttgccg tcctctatga tgatgtagct ggttttggtg cttgatttgg cagtcccgcc
     5941 gtcgcctttt atctcctctt ctttgctttc gtcatatatg taaacatact cttccgttct
     6001 gtccaaagta accattacct ttgtcctgcc cgctcccgac acggaggcaa ggaggtcggt
     6061 aagtttatct tcaagtattg actcgtagtc gccgatggag gggctttccg cgtcagaatg
     6121 tttctttttc gatttgccgc cgggcacaaa ctcggtgccc atgataataa gcataacggc
     6181 aacgccgatc agcataatcc ataacgcctt tttgtccttt tttaaattct caaaaagttt
     6241 actcaagctg catccgcctt tctgcgttcc cgagtgttta aaagccttat tcaaactttg
     6301 gtcttatgcc cgttagcttt tcaatttctt ccgccgcctg ttgtttttgc ggctctgtga
     6361 gttctgcctt aataactatg tccttaataa ttatgcggct gtcctccgca atatccgtaa
     6421 ttacctcgat ttcggagtaa tttagtccca aacttttaag tttttcccct actaagattt
     6481 ccactgccgc cttgccctgc ttttccatta ggtaattcat ttcatccctg acggtttccg
     6541 ctttatcctc aaggtcgatt ccaaaatctt cccgtctttt gggcaaatcg tcaaggtcaa
     6601 aggatttttc tataaagggc ataagtatca ctgacaaaag aaacagagaa agaagggtgt
     6661 tcataggctt ttccagtttg ccctttggaa tcagatacac gacgattgac gacgcgataa
     6721 agcaaattgc gaggtttacc gcccattctt ttattccttc catttgctcc tcccgttaac
     6781 cgttgctgat tgagaccaca agacccaatg aaacaatcat aagtaccgcg ttaaagcaga
     6841 gtatcgcgaa tatgacgcta agccctgagg caaggccgtc aatcattttt tgtatgctct
     6901 caatgccgaa catctccgag gcaaacgcgg cgccttttaa ggatataatc cacaaaagaa
     6961 tgtgtattac cgcggggatg ttgataaggg cgactatcag gattcccacc gctccgacgg
     7021 tggatttaac cacctgaagg ctgccgatta tggacgagag agcgtcgccg atggagcttc
     7081 cgataacggg aattgcggtg ccgattacga atttcgctcc ctttaaagca agggcgtcga
     7141 ctgagcccgc aagtattccc cttattgtca aaagacccgc gaatatggtg cccgcaaagc
     7201 tgaggactat ggttgtcgcc ttctttataa agttcgcgat gctttcgata ttgataatcg
     7261 ggtttatgct gcccgccatg ctgagcgcca tatatacccc ggagaagggg gttataaaac
     7321 tgttgcaaac ctgcgaggaa acctgggccg cggtaaaagc gatgccgttg tagctgagcg
     7381 ccgaagccgc cttgccagcc gcggcgataa gtcccgtcaa aatcggcacc aaggtcagca
     7441 taacctttga tgtggcgttt atagccgata ctgcgccgcc tatgcactga gaaattggct
     7501 tgattataat cattgccccg gcgaggacaa agacatatcc gaatatgctc cccttgccct
     7561 caagatccgg gatataacag ctgattaaag aaaacaggat aactatgccg accaaagaaa
     7621 acgcggcttt taggggctct tttaagcctc ctgtgactat tgagaagata aggtctataa
     7681 tctttctcgg cgacaaatca aagatatgct gagcgtcaaa atcttctatt cccagttctt
     7741 tcagcatttc cctcgtgtcg tcgtcgatga gattaaaaag ctcgtacgcc cctgcggatt
     7801 taagctgctc tgagtaaact tccgattcat caatctcaga gctttccgca tgcgcctgtg
     7861 cccctaaaag gggaagcaaa aagaggctta ttattattcc gcacagtatc tttttgactt
     7921 tgtttttcat agctcttccc ttattttcct ataagctcca aggcgaacct tgcgaccgag
     7981 atgagaaggg gcagagaaag cgtcagcact actgttttgc ccgcaagctc caccttgttg
     8041 gcaagcgcgc tttcgccgca gtcaatgcag gtgtcgtgcg caagttcggt tatgagagag
     8101 attccaagcg cctttgcaag aattgccaca tactcggtct ttactgagct tatgtcaatt
     8161 aggttttgaa aagtatccag aagctgcttt aaatctggca gaatcaaaag cagaattaca
     8221 acaacagctc cgagctgcaa aagtatggta aactcccttt tatgcaccca caaaagcaca
     8281 taaagaacgg aaat
//
LOCUS       DAONVQ010000088         8196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_6652_length_8196_cov_4.562216, whole genome shotgun sequence.
ACCESSION   DAONVQ010000088 DAONVQ010000000
VERSION     DAONVQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6652_length_8196_cov_4.562216"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLD98_06455"
     CDS             <1..422
                     /locus_tag="PLD98_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQA05921.1"
                     /translation="GAAIGASVAKGKAQTVLVTGDGSFGMNLTELATAVSQNLPLRIV
                     IMNNGVLGMVRQWQTLFFGKRYSNTTLNRKTDFVRLAKAFGAAGVRITDESELDSAMK
                     KAFQTDGPVVIDCIIDMDENVYPMIPAGGSIEDIILN"
     gene            434..949
                     /gene="ilvN"
                     /locus_tag="PLD98_06460"
     CDS             434..949
                     /gene="ilvN"
                     /locus_tag="PLD98_06460"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQA05922.1"
                     /translation="MRQDQFILGVIVSNHFGVLTRVSGLFSRRGYNIDSLNVSETEDP
                     KFSRMTIIVTGDDYIKNQIVKQLSKLRDVKKVELLPADATVLREHMLIKLDIKGHSIS
                     EITEAANIFRAKVIDFNADSVTIEMTGESSKLDAFIDYAKTFGIIELCRAGALAVKRG
                     PGALNGEYNED"
     gene            987..1979
                     /gene="ilvC"
                     /locus_tag="PLD98_06465"
     CDS             987..1979
                     /gene="ilvC"
                     /locus_tag="PLD98_06465"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HQA05923.1"
                     /translation="MAKMYYEKDCNLNVLSGKTVAVIGYGSQGHAHALNLHESGVNVV
                     VGLYEGSKSAERAREAGLTVKTTAEAVKSADVVMILVNDEKQAALYKKDIEPNLKSGA
                     ALAFAHGFNIHFNQIVPPSDVDVIMIAPKGPGHTVRSQFIEGKGVPCLVAVYQDATGS
                     AKEIALAYAAGIGGARAGILETTFKEETETDLFGEQAVLCGGVTALMKAGFETLVEAG
                     YQPESAYFECIHEMKLIIDLIIEGGMSFMRYSISDTAEYGDYTVGKRIITEETKKEMK
                     KVLEEIQSGEFATKWILENQANRPSFLAMRKQQQEHLCEKVGAELRKMMSWKKD"
     gene            2035..3702
                     /gene="ilvD"
                     /locus_tag="PLD98_06470"
     CDS             2035..3702
                     /gene="ilvD"
                     /locus_tag="PLD98_06470"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQA05924.1"
                     /translation="MRSDTVKKGPERTPHRALFKAMGYTDNQLKKPLIGIVNSFNEII
                     PGHIHLQTISRAVKDGVLAGGGTPIEFNVIGVCDGIAMGHRGMSYSLSSREVIADSIE
                     CMVMAHGFDALVFIPNCDKIVPAMLMAAARLNLPSIFVSGGPMLSLNDASGRGLDLNS
                     SFEAVGAYKSGKITEKELWEVEENCCPGCGSCSGMFTANSMNCLTEAIGMALPGNGTI
                     PAVYAKRIRLAKAAGEQIVETLEKNVRPLDVLTEKAFMNALAVDMALGCSTNSVLHLM
                     AVAHEAKVKLNLDIINEVSKRTPNLCKLAPAGKHHMQDLYNAGGVYKVMDVLLDAGLI
                     NADAMTAMGVTIGEAIKGQTYTITDPTVIRPIDNPYSKTGGIAVLRGNLAPDGAVVKQ
                     SAVDPEMLVHKGPARVFDSEEEAIKAIYNKEIKSGDVVVIRYEGPAGGPGMREMLSPT
                     SAIAGMGLDKEVALITDGRFSGATRGASIGHVSPEAAAGGPIAYIKDGDIISINIPEN
                     SLNVEISEEEFENRKKTTEIKKKTNLTGYIAKYARLVSSADKGAVIE"
     gene            3735..>4128
                     /locus_tag="PLD98_06475"
     CDS             3735..>4128
                     /locus_tag="PLD98_06475"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926774.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQA05925.1"
                     /translation="MERRIKIFDTTLRDGEQAPGCSMNLNEKIEVAKQLEILGVDIIE
                     AGFAISSPGDFKSVEVIAKELKNCTTASLARAVEKDIDAAYNALKHAVSPRIHVFLAT
                     SPIHMQYKLKMTPEQVLEKAVYAVKYAKK"
     assembly_gap    4129..4138
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4139..5240
                     /locus_tag="PLD98_06480"
     CDS             <4139..5240
                     /locus_tag="PLD98_06480"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQA05926.1"
                     /translation="IEFSAEDAMRSEPEFLAKVIEAVIDAGATTVNLPDTVGYCAPQE
                     IFDRVTYMRNNVPNIDKADISMHCHNDLGMAVANSFAGIMAGASQIECTVNGLGERAG
                     NAALEEIVMAIKTRKDLVNAYTNINTTQINKTSKLVYSIIGQSPALTKPIVGPNAFAH
                     ESGIHQHGVLAERSTYEIMSPEELGIPKRKLILGKHSGKHALIDRLEELGYSLSDAEF
                     EDFYKRFKELCDRKKVVTDSDIEALVNFKERAESKYTLEFFDVHTTKDSKSTCVIRLR
                     RDDEVFEEVSLGDGPINAAYNAIDKITGNVCEEMSSYSIHSVSDGNDALGEVTVRLRS
                     GDRTITGKGLSTDIIESSILAYINGINKLLGF"
     gene            5265..6833
                     /gene="cimA"
                     /locus_tag="PLD98_06485"
     CDS             5265..6833
                     /gene="cimA"
                     /locus_tag="PLD98_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HQA05927.1"
                     /translation="MNSEKRIEIFDSTLRDGAQSEGISFSVNDKLNIVKAIDDFGFDY
                     IEAGNPGSNPKDLDFFNQASKLKLKNAKLCAFGSTARYGIEPKDDANIKSLLSANTPA
                     VAIFGKSWDLHVTQILKISLENNLELIENTVSYLKSCGKEVIFDAEHFFDGYKENPAY
                     ALSALEAAVKGGADTLVLCDTNGGCMPLEVYEITKVVVEKYPDKKIGIHCHNDTGCAV
                     ANSMMAVEAGAVHVQGTFTGIGERCGNADLSIIVPNLKLKKGLNCSGDVSSLFNSARK
                     IAEIANTQIDNSKPYTGASAFAHKAGMHIDGVLKLSRSFEHINPEAVGNQRRFLMSEV
                     SGRSTVVEKIRYLAPHLSKDSPEIAEILKQLKELEHFGYHFEAADASFELLVRRVLGT
                     FTPHFKIVMFKTMGDYPVKEGTLPAYATVQVQVDGKEEITAEMGNGPVNALDLALRKA
                     LSVFYPQIRNVVLTDYKVRVLEQNSTTAAKVRVLIESSDDTGSWTTIGVSNDIIEASL
                     IALIDSLEYKLARP"
     gene            6853..8121
                     /gene="leuC"
                     /locus_tag="PLD98_06490"
     CDS             6853..8121
                     /gene="leuC"
                     /locus_tag="PLD98_06490"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQA05928.1"
                     /translation="MNKPMTMTQKILAAHAGKPYVEAGELVLVDLDMVLANDVTAPVA
                     INEFGRFGKENVFDKNKISLVMDHFAPNKDIKAAEQCKKCRDFANEKGIVNFFDGGEM
                     GVEHALLPEKGLVTAGDLVIGADSHTCTYGALGAFSTGVGSTDMACGMATGKAWLKVP
                     SAIKFVLKGKLNKWVSGKDVILHIIGKIGVDGALYKSMEYTGEGVAELSVSDRLCIAN
                     MAIEAGAKNGIFEVDDKTLQFLKEAGAKEPVIYKADEDAVYDEVYEIDLSEITPTVAF
                     PHLPSNVRTFDQIGEVKIDQVVIGSCTNGRLEDLVKAAEILKGKKVKKGVRVIVIPAT
                     QKIYLEAMEMGVLKTFIEAGALVSTPTCGPCLGGHMGILAKGERAVATTNRNFVGRMG
                     HPESEVYLASPEVAAASAITGRISEPKEVM"
     gene            8122..>8196
                     /gene="leuD"
                     /locus_tag="PLD98_06495"
     CDS             8122..>8196
                     /gene="leuD"
                     /locus_tag="PLD98_06495"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390421.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQA05929.1"
                     /translation="MNVKGKAIKYGDNIDTDVIIPARYL"
BASE COUNT         2334 a         2058 c         2100 g         1694 t
ORIGIN      
        1 tgggagccgc aatcggagcg tcggtagcga agggcaaagc tcaaacggta cttgtaacgg
       61 gcgacggcag tttcggaatg aatctgaccg agcttgccac agcggtatcc cagaacctgc
      121 cgctaagaat agtcatcatg aacaacggag ttttgggcat ggttcgtcag tggcagacac
      181 tgttcttcgg aaaacgctac tccaacacaa ctctgaacag aaaaacagac tttgtccgcc
      241 ttgccaaggc tttcggagcc gccggcgtca gaataacaga cgagagcgag cttgactcgg
      301 caatgaagaa ggcattccag acagacggcc cggtagttat cgactgcatt atagatatgg
      361 acgaaaatgt ttacccgatg attccggcgg gtggctctat cgaagacatt atattgaatt
      421 gaggttgagg aaaatgcgtc aggaccagtt catattggga gttatcgttt caaaccattt
      481 cggcgtgctg acaagggttt cgggattgtt ttcccgcaga ggctacaaca tcgacagcct
      541 taatgtcagc gaaaccgagg accccaagtt ttcaagaatg acaatcatag tcacaggcga
      601 cgactatatc aaaaaccaaa tagtaaaaca gctctcaaag ctcagggatg tcaaaaaggt
      661 cgagctattg cccgcggacg caaccgtgtt aagggagcac atgcttataa agctcgacat
      721 taaagggcat tccatttcgg agattaccga agccgcaaac atcttcagag caaaggttat
      781 agactttaac gccgactcgg tcacaatcga aatgacaggc gaaagctcca agctggacgc
      841 atttattgac tatgccaaga cctttggaat tatcgagctt tgcagggcgg gagctttggc
      901 ggtcaagaga ggcccaggcg ctttgaacgg cgaatacaat gaggattaat cagaacttaa
      961 attcttatat aaaggagtaa tggacaatgg caaaaatgta ttacgaaaag gattgcaatc
     1021 ttaatgtact cagcggaaaa acggtagcgg ttatcggcta cggcagtcag ggtcacgccc
     1081 acgcgttaaa ccttcatgaa agcggagtca atgtagttgt aggtctttac gagggctcca
     1141 agtctgcgga aagagcaaga gaagcaggtc tcaccgtcaa gacgacagca gaggctgtta
     1201 aatccgcgga tgttgttatg attcttgtca acgacgaaaa gcaggcagca ctttacaaaa
     1261 aggacattga gcccaacctc aagtcaggag cggcacttgc gttcgcacac ggctttaaca
     1321 ttcacttcaa tcaaatcgtt cctccctctg atgtggatgt tatcatgatt gctcccaagg
     1381 gccccggtca caccgtcaga agccagttta ttgagggcaa aggcgttccc tgccttgtag
     1441 ccgtatatca ggacgcaaca ggcagcgcaa aggaaatagc tctggcatac gcagcaggca
     1501 tcggcggcgc aagagcaggc atccttgaga ccaccttcaa agaggagacc gaaacagacc
     1561 ttttcggcga gcaggcagtt ctctgcggcg gcgtaaccgc tcttatgaag gcaggctttg
     1621 agacccttgt tgaggcaggc tatcagcccg aaagcgcata ctttgagtgc atccacgaaa
     1681 tgaagctcat catcgacctt atcatagagg gcggcatgtc cttcatgcgc tactcaatca
     1741 gcgatacagc cgaatacggc gactacaccg ttggcaagag aatcatcacc gaagagacca
     1801 agaaggaaat gaagaaggtc cttgaggaga ttcaaagcgg cgaattcgct accaagtgga
     1861 tactcgagaa tcaggccaac agaccttcat tcctcgcaat gcgcaaacag cagcaggagc
     1921 acctgtgcga gaaggtcggc gccgagctca gaaagatgat gagctggaag aaagactaac
     1981 agtctgcacc ctaaataaaa agaaaagagg gcgagcgttt tgggtggtgt aaatatgaga
     2041 agcgacacag ttaaaaaagg ccctgaaaga actccccaca gagctctttt caaggcaatg
     2101 ggatatacgg ataatcagct taaaaagccg cttataggca tagtaaactc ctttaacgag
     2161 attatccccg gacatatcca ccttcagaca atttccagag cggttaagga cggtgttctt
     2221 gccggcggcg gcactcccat tgagttcaat gtcataggag tatgcgacgg aatcgccatg
     2281 ggtcacaggg gcatgagcta ttccctttca tcaagggagg taatagccga cagcattgag
     2341 tgcatggtta tggctcacgg ttttgacgcc cttgtattta tccccaactg cgacaagatt
     2401 gtccccgcca tgcttatggc agcggcaagg ctaaatctcc ccagcatttt cgtttccggc
     2461 ggtcccatgc tgtcacttaa tgacgcgagc ggacgcggac ttgaccttaa cagttccttt
     2521 gaggctgtcg gagcatacaa gagcggcaaa atcaccgaaa aggagctttg ggaggtagag
     2581 gagaactgct gccccggctg cggctcctgc tcaggtatgt tcacagcgaa ctcaatgaat
     2641 tgccttaccg aagcaatagg catggcgctg ccgggcaacg gcacaatccc cgcggtatac
     2701 gcaaagagaa tcagacttgc gaaagccgca ggcgagcaga ttgtcgaaac cttggagaag
     2761 aatgtccgcc ccttagatgt tctgaccgaa aaagcattta tgaacgccct cgcagtagat
     2821 atggcgctcg gctgctccac aaactccgtt ctgcacctta tggcagtggc gcacgaggcc
     2881 aaagttaaac tgaatctcga cattatcaac gaagtcagca agagaacacc caatctctgc
     2941 aaactggcac ccgcgggcaa gcaccacatg caggaccttt acaacgctgg cggcgtctat
     3001 aaggttatgg atgttctatt agacgcaggt cttataaacg ccgacgccat gaccgccatg
     3061 ggagtaacga taggtgaggc aatcaagggt cagacctaca ccattaccga cccgacagta
     3121 atcagaccca tcgacaaccc ctattccaaa acaggcggca tagcggtatt aagaggcaac
     3181 cttgcgcccg acggagcggt cgtaaagcag agcgccgttg accccgaaat gctcgttcac
     3241 aagggacccg caagggtgtt tgacagcgag gaagaggcaa tcaaggccat atataataag
     3301 gaaataaaga gcggagatgt tgtggtaatc cgctatgagg gtcccgcagg cggtcccggt
     3361 atgagggaga tgctttcccc cacctccgca atagcgggca tgggacttga caaggaggta
     3421 gcccttatta ccgacggtcg tttctcaggc gccacaaggg gagcctcaat aggccatgtt
     3481 tcgcccgagg cggcggcagg cggcccgatt gcctacatca aagacggaga tatcatcagc
     3541 atcaatatcc ccgagaacag cttgaatgtt gagatttccg aagaagagtt cgaaaacaga
     3601 aagaaaacaa ccgagattaa gaaaaagaca aatcttacag gctacatagc aaagtacgca
     3661 agacttgttt catctgcgga taaaggcgca gtaatagaat aacactacaa cgacgataag
     3721 gaggagaagc ggctgtggaa aggcgaatta agatattcga tacaaccttg agagatggag
     3781 aacaggctcc cggctgcagc atgaatttaa acgaaaaaat cgaggtagca aagcagttag
     3841 agattttggg cgttgacatt atcgaagcag ggtttgcaat ctcatctccc ggcgacttta
     3901 agtcagtgga ggtaatagca aaagagctaa agaactgcac cactgcctcc cttgcaagag
     3961 ctgttgaaaa ggatatcgat gcggcgtaca acgctttgaa gcatgccgtt tccccgagaa
     4021 tccatgtttt ccttgcgacc tcgccgatac acatgcagta caaactcaaa atgacccccg
     4081 agcaggtgct tgaaaaggct gtctacgcgg ttaaatacgc aaagaaatnn nnnnnnnnca
     4141 ttgagttctc tgcagaggac gcaatgcgca gcgaacccga attccttgca aaggtcatcg
     4201 aggcagtaat agacgcggga gcaaccaccg tgaatctccc cgacacggta ggctactgcg
     4261 ctccccagga gatattcgac agggttacat atatgagaaa caatgtgccc aacatagata
     4321 aggcggatat ctccatgcac tgccataacg acctcggcat ggcagtggca aactcctttg
     4381 cgggtattat ggcgggcgcg tctcagattg agtgcaccgt caacggtctg ggcgaaaggg
     4441 caggcaacgc ggcccttgag gaaatcgtaa tggcaatcaa gacccgcaag gaccttgtaa
     4501 atgcctacac aaacatcaat accactcaga ttaacaaaac cagcaagctg gtttacagca
     4561 tcataggtca gtcgcccgct ctcacaaagc ccattgtcgg acccaacgcc tttgcccatg
     4621 aatcgggcat ccaccagcac ggcgttctgg cagagcgttc gacctatgag attatgtccc
     4681 ccgaggaact gggcatcccc aagagaaagc tcatactcgg caagcactcg ggcaagcacg
     4741 cccttattga cagacttgaa gagctgggct acagcctttc cgacgccgag ttcgaggatt
     4801 tctacaagag attcaaggag ctctgcgaca gaaaaaaggt tgttaccgac agcgatatag
     4861 aagcccttgt aaattttaag gagagggcag agagcaaata tacactcgag ttcttcgatg
     4921 tccataccac caaggattcc aagtccactt gcgtaataag gctcaggcgc gacgacgagg
     4981 tattcgaaga ggtttccctc ggcgacggcc cgattaacgc cgcgtacaac gccattgata
     5041 agattacggg caatgtctgc gaggagatga gcagctactc gattcactcc gtcagcgacg
     5101 gcaacgacgc tctgggcgaa gttacggtca gactgcgctc aggcgacaga acaattacag
     5161 gcaaaggact ttcaaccgac attatcgagt ccagtattct tgcgtatatt aacggaatca
     5221 acaaacttct aggtttttga gcttcgtttg gagggtgcaa attaatgaac agcgaaaaaa
     5281 gaatagaaat ctttgactca acgctacgcg acggagccca aagcgaagga atctcatttt
     5341 ccgtaaacga taagctgaac atcgtaaaag caattgacga tttcggtttt gactatatag
     5401 aggcgggcaa ccccggctca aacccaaagg atttggattt cttcaatcag gcgtcgaagc
     5461 tcaagctcaa gaacgcgaag ctatgcgctt tcggcagcac ggcaaggtac ggaattgagc
     5521 ctaaagacga tgccaacatc aagtcgctat tgagcgccaa tacccccgcc gttgcgattt
     5581 tcggcaagtc gtgggatctg catgtaaccc aaatccttaa aatcagcctt gagaataatc
     5641 ttgagcttat cgagaacacc gtttcttacc ttaaatcctg cggcaaagag gtaatttttg
     5701 acgccgagca tttctttgac ggatataagg aaaaccccgc ttacgccctc tcagcccttg
     5761 aggctgccgt aaagggcgga gcggacacac ttgtgctctg cgacaccaac ggcggctgca
     5821 tgccccttga ggtctacgaa atcacaaagg ttgttgtaga gaaatatccc gataagaaaa
     5881 tcggtatcca ctgccacaac gacactggct gcgccgtagc caactccatg atggcggttg
     5941 aggcgggagc ggttcatgtt cagggcacct ttacgggaat cggcgaaagg tgcggcaacg
     6001 cggacctgag catcatagtc cccaatctca agctcaaaaa gggtcttaac tgctcgggcg
     6061 atgtttcttc gctctttaat tccgcaagaa agattgccga gattgccaat acacagattg
     6121 acaactcaaa gccctacacc ggggcaagcg cctttgctca taaagcgggc atgcatatcg
     6181 acggagtttt gaagctctcc cgctcattcg agcacatcaa ccccgaagcc gtcggcaacc
     6241 agaggcgttt ccttatgtcc gaagtttcgg gcagaagcac cgttgtggag aagataaggt
     6301 acttagcgcc tcatctttca aaggattctc ccgaaattgc cgagatttta aagcagctta
     6361 aagagcttga gcatttcggc tatcatttcg aagcagccga cgccagcttt gagctgcttg
     6421 taagaagagt tctgggtacc tttacgcccc acttcaaaat cgtcatgttc aaaaccatgg
     6481 gcgactaccc cgtaaaggag ggcaccctgc ccgcctatgc gacggttcag gttcaggttg
     6541 acggcaagga ggaaatcaca gcggagatgg gcaacggtcc cgtcaacgct ctcgaccttg
     6601 cgctcagaaa agccctttcg gttttctacc cgcaaatccg caatgttgtg ctcaccgact
     6661 ataaggttcg cgtgctcgag cagaacagca cgacagccgc aaaggtaagg gttcttatcg
     6721 aaagctccga cgatacaggt tcatggacaa caataggcgt ttccaacgat attatagaag
     6781 ccagcttgat agctctcatc gattccttag agtataagct ggcacgacct taaactttcg
     6841 ggaggtaaaa ctgtgaataa accaatgaca atgacccaaa agattcttgc agcccacgca
     6901 ggaaagccct atgtcgaagc gggcgagctg gttctcgtcg accttgacat ggttcttgcc
     6961 aacgatgtta ccgcgcccgt cgcgataaac gagttcggca ggttcggcaa agaaaatgtt
     7021 ttcgacaaaa acaaaatctc ccttgttatg gaccactttg ctcccaacaa ggatattaag
     7081 gcagcagagc agtgcaagaa gtgccgcgac tttgcgaatg aaaaaggcat tgtaaacttc
     7141 tttgacggcg gagaaatggg cgttgagcac gcgcttctgc ccgaaaaggg acttgtaacc
     7201 gcaggcgacc ttgtaatcgg cgcggactcc cacacctgta catacggcgc tctgggcgct
     7261 ttctcaacag gcgttggctc caccgatatg gcatgcggca tggcaacggg caaggcatgg
     7321 ctcaaagttc cctcggcaat taagttcgtt ctcaaaggca agctgaacaa atgggtatcc
     7381 ggcaaggatg taatactcca catcatcggc aaaatcggcg ttgacggagc cctctacaaa
     7441 tcaatggagt acacaggcga gggcgtagcc gagcttagcg tcagcgacag gctctgcatt
     7501 gccaacatgg caattgaggc aggcgcaaag aacggcatct ttgaggttga cgacaagact
     7561 ctccagttcc tcaaagaggc aggcgcaaag gagcccgtca tctacaaggc tgacgaggac
     7621 gccgtatatg atgaggttta cgagattgac ctcagcgaaa ttacccccac agtagcgttc
     7681 ccgcaccttc cctccaatgt ccgcaccttt gaccagatag gcgaagtcaa gatagaccag
     7741 gttgtcatcg gctcatgcac aaacggcaga cttgaggacc ttgtaaaggc tgccgaaatc
     7801 ttaaagggca aaaaggtcaa aaagggcgtc agggtcatag tcattcccgc cacacagaag
     7861 atttacctcg aggctatgga aatgggcgtc ttaaagacct ttatcgaagc tggcgctctc
     7921 gtttcaacac ccacatgcgg cccctgcctt ggcggacaca tgggcattct cgccaagggc
     7981 gaaagggcag tagccaccac caacagaaac tttgtcggca gaatgggcca ccccgagtca
     8041 gaagtctatc tcgcaagccc tgaggttgcc gcagcaagcg caatcaccgg ccgcatttcc
     8101 gagcctaagg aggttatgta gatgaatgtc aaaggcaaag caataaaata cggcgacaac
     8161 atcgacaccg atgttataat ccccgcaagg tacctt
//
LOCUS       DAONVQ010000089         8194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_6656_length_8194_cov_4.066716, whole genome shotgun sequence.
ACCESSION   DAONVQ010000089 DAONVQ010000000
VERSION     DAONVQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6656_length_8194_cov_4.066716"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PLD98_06500"
     CDS             complement(<1..161)
                     /locus_tag="PLD98_06500"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQA05930.1"
                     /translation="MSTIAAIATPLAAGGLGVIRISGEDAINIADKIFRPYSGKSLKE
                     MKGYTAAYG"
     gene            complement(183..1430)
                     /gene="glyA"
                     /locus_tag="PLD98_06505"
     CDS             complement(183..1430)
                     /gene="glyA"
                     /locus_tag="PLD98_06505"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQA05931.1"
                     /translation="MYHCYNGQFDLIAKEDPQVAEAMEAELSRQRRNIELIASENIVS
                     PAVMAAMGSILTNKYAEGYPGKRYYGGCQCVDVVEDIAINRAKELFGAEHANVQPHSG
                     AQANMAVYFALLQPGDTVLGMSLAHGGHLTHGSPVNISGKYFNFHSYGVNDDGFIDYD
                     ALEKTAKEIRPKLIVAGASAYPRIIDFERISNIAKEIGAYFMVDMAHIAGLVAAGLHP
                     SPVPYADVVTSTTHKTLRGPRGGLILCKEELGAAINKAIFPGTQGGPLMHVIAAKAVC
                     FGEALRPEFKEYQQRVVNNSKVLASALLEKGFDLVSGGTDNHLMLVDLRPMKITGKEL
                     EHRLDEVFITVNKNAIPNDPEKPFVTSGIRIGTPAVTTRGFGEEEMKTIAQLIFDTAT
                     DFEGTADKTRETVNDICRRFPLY"
     gene            complement(1606..2631)
                     /gene="spoIID"
                     /locus_tag="PLD98_06510"
     CDS             complement(1606..2631)
                     /gene="spoIID"
                     /locus_tag="PLD98_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HQA05932.1"
                     /translation="MKVFAVLCLITTVILTLTPLTAVNKKDINPPKQEETTEKHTTEE
                     TSTTEKVTEETKPPEEIEVLAVASGNVVTMNFLDYIIGVVAGEMPASYHEEALKAQAV
                     AAYTYALRNIYNERENPSPALKGASITDDSTKHQAYITVDKMKEKWGDNFDTNYKKIA
                     NAVKEVFGEVVTYNGQIANTLFHSISSGKTESAKNIWGGSGVPYLVSVDSTGDVFAPN
                     YSSVTVLSKEQFKNIAKTIPSVKLSDNPKEWVGEAEKTTAGTVTSITIGGVKLKGTEV
                     RAYFGLRSPAFTITYKDGNFTIKTTGYGHGVGMSQYGADYMARKGKTYREILAHYYPG
                     TEIVRFD"
     gene            2788..4218
                     /locus_tag="PLD98_06515"
     CDS             2788..4218
                     /locus_tag="PLD98_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HQA05933.1"
                     /translation="MHSYISPISNESLSILCKSLMDNNHIDQDDFEFYNVKRGLRNQD
                     GSGVMAGLTRICSVDGYYIADGEKQPKEGRLIYRGINVEEIVEDCVKNDRFGFEEVVW
                     LLLFGSLPTKERLDQFCETLSKCRELPANFVEDVIMKLPSPNIMNKLAHSVLALYPYD
                     ETPDDNSIPNVLRQSIQLVAQIPIIMSYAYQVKRKNYYHESMYIHQVKPEHRTAEVIL
                     RAIRSNKQFTDKEAKLLDRCLILHAEHGGGNISTFATRVLTSAETDTYSAISAGIGSL
                     KGHKHGGANLKVAEMVEDIKEHVSDITDPGQVADYITKIVKKEAGDRSGLVYGMGHAV
                     YTYSDPRAVILKEEAKKYLPEAPEFEDELRLLELIEELTPEIFKKLKGSNKVICANVD
                     LYSGLVYKMLRIPKDLFTPLFTVARLPGWCAHRIEEIITGGRIIRPAYKSLFESMDYI
                     PLNERQEDIVVKAKKHGPKSNSFLYL"
     gene            4271..5329
                     /locus_tag="PLD98_06520"
     CDS             4271..5329
                     /locus_tag="PLD98_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05934.1"
                     /translation="MKLKGSQNAIHPDALLILFAIASLFLLPLRTYQLIKIVDPQTGF
                     FTDGANPTIIILYTIIALVTFILLAASFLSANIPASIPPSGKKRPLGIVSAVFAVSFL
                     VDAIVQITSVVVMFGDKTETSLPTLTARNAWFPVALQAFFALVSTVYFIAVAISYISG
                     KTSYTNNRLMALAPLLWTFCRMIYRFMRAISFVRVSELLLEICAIAFLIIFFMTFARI
                     SSDVNRKGTMWIIFGAGLPAAMLLLCVSVPRIVLVLTGNSKMLVNHSPLEYADFPAAL
                     FIIAYILTVLRSGYKTEAELEAEEAEKAEEGAETEGEEEPKEETEEPAELSETDKKES
                     SENKIPFDRSQLIDELKD"
     gene            5423..6355
                     /locus_tag="PLD98_06525"
     CDS             5423..6355
                     /locus_tag="PLD98_06525"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQA05935.1"
                     /translation="MFWDNVLTAGTQVLILYIIVFLGFLAERLNVYSEKTAKATTNFL
                     FYFITPAVIINAFLSTEFTREKALSYGTAFLCGVATHVGAILLSAPFFRRGKHPDNAV
                     YKYAAVYANMGYMALPLAEAVVGPEGVFYCSAGVITFNIFSFTHGVWLMESDDKDKKF
                     NIKSLILNPGVLSVMVGLPLFLFSVKLPIIIQKPISYMASMNTPLAMIILGTYISKAD
                     FKTIFKDTRLFGVLFLKLIAVPLLMFGIFKLAGVSGALLTSVLISASSPSASNTVMFS
                     AKYGKNTRTASKIVSFVTFFSILTMPIIIALSRL"
     gene            6383..7753
                     /locus_tag="PLD98_06530"
     CDS             6383..7753
                     /locus_tag="PLD98_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQA05936.1"
                     /translation="MYSPILNAIQEHINKGRISFHTPSHKGAEGSYLSFFADFLKYDL
                     TEIPDTVGLFANSGPVYEAMELAAKAFGTAATLFSAGGCTHAIQAMLRLACKEGGKII
                     AGRNLHPAAVNAMALLDIDPVFIQPEMEGKCALGGRISPDAVERKLSKNKDAQVVYIT
                     SPDYFGIMSDIAAISGVAKKYGVPVIVDNAHGAHLLYAADNLHPIRLGAAMSADSSHK
                     TLPVLTAGAWLQIADEKYKADALDAMRLFGSTSPSFPVLLSLDLCRAWLEEKGAEEFN
                     KLKTRLESVKAALNETPFIKPEGLVDPYRIAFDASCLNRSDDEIYEFFHSRNIEPEFI
                     SRGAAVFIPSPFNTENEINTLAKAITELQSIQKAKGNSDVSYAAPHLPNKAMTIRQAV
                     MAESEYVPIEKAKGRIAAAAEFICPPGFALTIPGEIIDDESVENLYRASIFNIKVVKS
                     GAVKYN"
     gene            7795..8121
                     /locus_tag="PLD98_06535"
     CDS             7795..8121
                     /locus_tag="PLD98_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HQA05937.1"
                     /translation="MKLVIAIVSNDDSHTVLSALTKANFSVTKLSSSGGFLRVGNTTL
                     MIGTEAERLEELKDLLREYCSTRQLATVSMPASYSDAMFSMLPVEVTVGGATVFVLDV
                     EEFLKI"
BASE COUNT         2103 a         2070 c         1888 g         2133 t
ORIGIN      
        1 tctccgtaag cggcggtgta gcccttcatt tctttaaggc tctttccgct gtatggtctg
       61 aatatcttgt cggcaatatt tatggcatcc tcgcccgaaa ttcttataac tccgagtccg
      121 cctgctgcca gcggggtggc aattgcggca atagtgctca tccaaagttc acctgttttt
      181 gtttagtaga gagggaatct cctgcagata tcatttactg tttctcttgt tttatcggca
      241 gtgccctcaa aatcagtggc ggtgtcgaat atgagctgag ctatggtctt catttcttcc
      301 tcgccaaagc cccttgtggt aacggcgggg gtgccgattc tgattccgct tgttacgaac
      361 ggcttttcgg ggtcgttggg gatggcgttc ttgtttactg taatgaaaac ctcgtccagc
      421 ctgtgctcaa gctctttgcc cgtaatcttc atgggtctga ggtcaacgag cataaggtgg
      481 ttgtccgtgc cgccggatac aaggtcaaag cctttttcta agagagcgct tgcaagaacc
      541 ttgctgttgt ttacgactct ttgctgatac tctttaaact cgggtcttaa agcctcgccg
      601 aagcaaacag ccttggcggc gattacatgc atgaggggac cgccctgagt gccggggaat
      661 atggccttgt ttatagcggc gcccagctcc tctttgcaga gaataagtcc gcctctggga
      721 cctcttaatg tcttgtgagt ggtggatgtg acaacatcgg cgtagggaac ggggctgggg
      781 tgcagacccg ccgctacaag accggcgata tgagccatgt ccaccataaa gtaagcgccg
      841 atttcctttg cgatgttgga gatgcgctca aaatctataa ttctcggata tgccgacgcg
      901 cccgctacaa taagcttagg tctaatctcc ttggctgtct tttcaagggc gtcgtagtcg
      961 ataaatccgt catcgtttac gccgtaagaa tggaaattaa agtacttgcc cgaaatgttt
     1021 acgggtgagc cgtgggtaag atgtccgccg tgagcaaggc tcatgcccag aaccgtgtcg
     1081 cccggctgaa gcagagcgaa atataccgcc atatttgcct gggcgccgga atggggctga
     1141 acattggcgt gttctgcgcc gaaaagctcc ttggctctgt taattgcgat atcttccacg
     1201 acatcaacgc actggcagcc gccgtaatat ctcttgccgg ggtagccctc agcgtacttg
     1261 tttgtaagaa tgctgcccat agccgccata acagcggggg aaacaatgtt ttccgaggca
     1321 ataagctcaa tgttcctgcg ctgacggctg agctcagcct ccatagcctc ggcaacctgc
     1381 gggtcctctt tggctattaa atcaaactga ccgttataac agtgatacat gctttccatc
     1441 ccttcatttt tcaaggaaaa ggtttagccc atgccaattc cttaatatgt ttgcaatttc
     1501 ttcaaattct atcacgaatc actgttaatt tcaacaaata ttgacctttt aaaaaataaa
     1561 aagccgatgc cgcggcatcg actctttgtt aaatttaacc aaattctaat cgaaccttac
     1621 aatctccgta ccggggtagt agtgggcaag aatttctctg tatgtcttgc cttttcttgc
     1681 catatagtcg gctccgtact gactcatgcc gactccgtga ccgtagcccg ttgtcttaat
     1741 ggtgaaattt ccgtctttgt aagttatcgt aaacgcgggg cttctaagtc caaaatatgc
     1801 ccgaacctcc gtacccttga gctttactcc gccgattgta atggatgtta ccgttcccgc
     1861 agtggttttc tcggcttctc cgacccattc ctttgggttg tctgagagct ttaccgacgg
     1921 aattgtcttt gcaatattct taaactgctc ttttgaaagc accgtaaccg agctgtaatt
     1981 cggagcgaat acatctcccg tgctgtcaac cgaaacaaga tagggaacgc cgctgccgcc
     2041 ccagatgttc tttgcgctct cggtttttcc cgagcttatg gaatgaaaaa gagtatttgc
     2101 aatctgtccg ttgtaggtca caacctcacc gaaaacctct ttcaccgcgt ttgcgatctt
     2161 tttgtagttt gtgtcaaaat tgtctcccca tttttccttc atcttgtcaa cggttatgta
     2221 agcctgatgt tttgtgctgt cgtcggtaat tgacgcgcct ttaagggcgg gggaggggtt
     2281 ttctctctcg ttgtatatgt ttctcagagc gtatgtatag gcagccacag cctgagcctt
     2341 cagcgcctcc tcatggtagg aggcgggcat ttcacccgcg actactccga ttatgtagtc
     2401 aagaaaattc atcgtcacca catttccgct ggcaacggcg agaacctcaa tctcctccgg
     2461 gggctttgtt tcctccgtaa ccttttcggt agtgctcgtc tcctcggtcg tatgcttttc
     2521 cgtggtttcc tcctgctttg ggggattaat atcttttttg tttaccgctg tcaggggagt
     2581 taaagtgagg atcacggtgg ttattaagca aagcactgca aataccttca ttttgctgcc
     2641 tcctgtaatg tttgtccgct gcgtgactaa aataaaagga ataaaaagct tttatggcta
     2701 aataaatata agtaaatccg cgcgcttatt gactttaagg cgccgatata atagaataag
     2761 caataaattt aatttggaga tggtccgatg cactcatata tttcccccat ttcaaatgaa
     2821 tccttgtcta ttctatgcaa gtccctgatg gataataacc acatagacca ggatgatttt
     2881 gagttttaca atgtaaaaag gggtctcaga aatcaagacg gttcaggcgt tatggcaggt
     2941 ctgacccgca tttgcagtgt tgacggatat tatattgcgg acggcgaaaa gcagcccaaa
     3001 gaaggcaggc ttatatatag aggcattaat gtcgaggaga tagttgaaga ctgcgtcaag
     3061 aacgacaggt tcggctttga ggaggtcgta tggctgctgc ttttcggcag tctgcccact
     3121 aaggagcgtc ttgaccagtt ctgcgaaacc ctctcaaagt gcagggaact gcccgcaaat
     3181 tttgtagagg atgtaataat gaagcttccg tcgcccaata ttatgaataa gctggcgcat
     3241 tccgtgctgg cgctgtaccc ctatgacgaa acccccgacg acaactcaat ccccaatgtt
     3301 ttaaggcagt caattcagct cgtggctcaa atccccatca tcatgtcata cgcttatcag
     3361 gtaaagcgca aaaattacta ccacgagagt atgtatatac atcaggtaaa gcctgagcac
     3421 cgcaccgctg aggttatcct cagagccatc cgttccaaca agcagtttac cgacaaggag
     3481 gcaaaactcc ttgaccgctg ccttattctc cacgccgagc acggcggcgg caacatctcc
     3541 acctttgcaa caagagttct gacctcagcc gagacagata cctattccgc aatcagcgcg
     3601 ggtatcggct cacttaaagg tcacaagcac ggcggagcca acctcaaggt tgccgaaatg
     3661 gtcgaggata ttaaggaaca tgtaagcgac atcaccgacc ccggtcaggt tgcggactat
     3721 attacaaaga tagtaaagaa agaggcaggc gaccgttcag gtcttgtata cggtatgggc
     3781 catgccgtat atacctattc cgacccgaga gcggttatct taaaagagga ggcaaagaaa
     3841 tacctccccg aagctcccga atttgaagat gagcttagac ttcttgagct tatcgaagag
     3901 ctcacccccg aaatattcaa gaaactgaag ggcagcaaca aggtaatctg cgctaatgtg
     3961 gacctttatt ccggccttgt atacaagatg ctcagaatcc ccaaggattt gttcacaccc
     4021 ctctttacag tggcaagact gcccggctgg tgcgctcaca gaattgagga aattatcacc
     4081 ggcggacgca ttatccgtcc cgcgtacaag agcctgtttg agtccatgga ctatatacct
     4141 ctgaacgaga gacaagagga tatagtcgta aaggcaaaaa agcacggccc caagagtaac
     4201 agcttcctgt acttgtaaag caaatcagac tgattcaatc agccttaatc tgaattaagg
     4261 ggaaagaata atgaaactta aaggttctca aaacgccatt catcccgacg cgctgctgat
     4321 actgtttgcg atagcttccc ttttccttct tccgcttaga acatatcagc tcataaaaat
     4381 agtggacccg caaacaggct tttttaccga cggcgcaaat cccactataa taattctcta
     4441 tacaataatt gcgctggtaa cctttatact tcttgcagcg tcatttcttt cggcaaatat
     4501 ccccgcgtca atccccccgt cgggcaaaaa aagacctctg ggcatcgttt ccgcagtctt
     4561 tgcggtatcc ttcctggttg acgcgatagt gcagataacc tccgttgtgg taatgttcgg
     4621 cgacaagact gaaacctcac ttcccacact caccgcccgc aacgcatggt tccccgtagc
     4681 actccaggct ttctttgccc ttgtttcaac ggtctacttc atagcggtcg caatatcgta
     4741 tatttcgggc aaaacctcat acacaaacaa ccgccttatg gctcttgccc cgcttctgtg
     4801 gaccttctgc agaatgattt accgctttat gagggcaatc agttttgtaa gggtctccga
     4861 gcttttactc gaaatctgcg cgatagcgtt ccttatcatc ttctttatga cctttgcccg
     4921 catctcctcc gatgtcaacc gcaagggcac aatgtggata attttcggag cgggtctccc
     4981 cgccgcgatg ctcctgctgt gcgtatcggt gcctaggata gttcttgtcc tcacgggcaa
     5041 cagcaagatg cttgtaaatc attcacctct tgaatacgca gacttccccg cagccctgtt
     5101 cataatcgcc tatatcctga ccgtcctccg ctcaggctat aagacagagg cagagcttga
     5161 ggctgaagag gctgaaaagg ctgaagaggg cgcagaaaca gaaggcgagg aagagcctaa
     5221 ggaagaaaca gaagaacccg ccgagctttc cgaaacagat aagaaggaaa gctctgagaa
     5281 taaaatcccc tttgacagaa gtcagcttat tgacgagctg aaagattaag gatacttaca
     5341 atttgtcgca atatgcggcg gcataagagt gctgccgcat aattctatgt aaagcctaat
     5401 taatattaag aggtgcaacg ccttgttttg ggataatgtt ttaactgcgg gaacgcaggt
     5461 tttaatactt tacataatag tctttctggg ttttctggct gaaaggctga atgtctactc
     5521 cgaaaaaacc gcaaaggcaa cgacaaactt cctcttttac ttcataactc ccgcggttat
     5581 aatcaacgcc tttttgtcaa cagagtttac tcgtgaaaag gcgctttcct acggcacagc
     5641 ctttctgtgc ggagtagcga cccatgtggg agctatactg ctctcagctc cctttttcag
     5701 aaggggcaag caccccgaca acgcggtata taagtacgcg gcggtctacg caaacatggg
     5761 ctacatggct ctgccccttg ccgaggctgt cgtcggtccc gagggagtat tctactgctc
     5821 agcgggcgtc ataaccttca acattttctc cttcacccac ggcgtgtggc taatggagag
     5881 cgacgacaag gacaaaaagt tcaacattaa atccttaatc cttaaccccg gcgttctttc
     5941 cgtcatggta ggtctcccgt tgtttttgtt ttctgttaag ctcccgataa taatacaaaa
     6001 gcccatttcc tacatggcga gtatgaacac gccccttgcg atgataattc tcggaacata
     6061 tatctcaaag gcggacttta aaaccatatt taaggacaca aggcttttcg gcgtgctttt
     6121 cctcaaactc attgccgtcc ccctccttat gttcggaata tttaagctgg cgggagtcag
     6181 cggggcgctt ttaacctcgg tgcttatctc cgcctcatcg ccctccgctt caaatacggt
     6241 aatgttttca gcaaagtacg gaaaaaacac aagaaccgca tcaaagattg taagctttgt
     6301 aaccttcttc tcaatcctga caatgcccat aatcatcgca ttgtcaaggc tgtagccgca
     6361 tttttaagct gcgggtgatt gaatgtattc accgattttg aacgcgatac aagaacacat
     6421 aaataaaggc aggatcagct ttcacactcc gtcgcacaag ggagcggaag gctcttacct
     6481 gtcttttttt gctgactttc tcaaatacga tttaaccgaa atccccgaca cagtcggact
     6541 tttcgcaaac agcggtcccg tctatgaggc catggagctc gccgccaaag cattcggcac
     6601 cgccgccaca cttttcagcg cgggaggctg cacccatgcc attcaggcta tgctgcgtct
     6661 tgcctgcaaa gagggcggca aaataatagc gggcagaaat ctccaccccg cagccgtaaa
     6721 cgccatggct ttgctcgata ttgaccccgt tttcattcag ccggaaatgg aaggaaaatg
     6781 cgccctcggc ggcagaatca gccccgacgc tgtggagcgg aaacttagca aaaacaaaga
     6841 cgcgcaggtg gtctacatta cctcgcccga ctatttcggc attatgtccg atatagccgc
     6901 aatctcgggc gtggcaaaaa aatacggagt gcccgttatc gtagacaacg cccacggcgc
     6961 gcatctgctt tacgccgctg ataaccttca ccccattaga ctgggagcgg caatgtcggc
     7021 tgactcctca cacaaaaccc tgcccgttct gaccgccggc gcatggcttc aaatcgcaga
     7081 cgaaaaatat aaagcggacg cccttgacgc catgcggctc tttggctcca ccagcccatc
     7141 cttccccgtg ctcttatccc tagacctttg cagagcatgg ctggaggaaa agggggcaga
     7201 ggaatttaat aaactcaaaa cccgcctgga atccgtcaaa gcagccttaa acgaaacccc
     7261 cttcattaaa cccgagggtc ttgtcgaccc atatagaata gccttcgacg cctcttgcct
     7321 gaataggtct gacgatgaaa tctatgagtt tttccactcg agaaatatag aacccgaatt
     7381 tataagcagg ggagcggcgg ttttcatccc ttctccgttt aatacagaga acgaaatcaa
     7441 tacactggca aaggcaataa ccgagcttca gagcatacaa aaagcaaaag gcaattcaga
     7501 tgtttcatac gctgcgccgc atcttcctaa caaggctatg acaataagac aggcggtaat
     7561 ggcagagagc gaatatgtcc caatagaaaa ggcaaagggc agaatcgccg ccgccgctga
     7621 atttatctgc ccgcccggct ttgccctcac aattcccggg gaaataatag acgacgaatc
     7681 cgtggaaaat ctttatagag caagtatttt taatattaaa gttgtaaaat caggcgcggt
     7741 aaagtataat taaaatacgg acattccgta aaagcccgta tgaggaggat aattatgaag
     7801 ctggttatcg ccatagtaag caatgatgac agccatacgg ttttatccgc actgacaaag
     7861 gctaacttca gtgtaacaaa gctctcctcg agcggcggct ttcttcgtgt cggcaacacc
     7921 accttaatga taggtacgga ggccgaaagg ctggaagagc taaaggattt gttaagagag
     7981 tattgcagca cccgtcagct ggcaactgtt tcaatgcctg caagctattc ggacgcaatg
     8041 ttttcaatgc tccccgttga ggtaacggtc ggcggcgcca ccgtctttgt cctcgatgtg
     8101 gaagagtttt taaagattta agaatgaata ccaagagtat aaaactgtcg gaagttaaac
     8161 ttaaagattt atcggcagag gttgacagcg gcag
//
LOCUS       DAONVQ010000090         8109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_6753_length_8109_cov_4.391482, whole genome shotgun sequence.
ACCESSION   DAONVQ010000090 DAONVQ010000000
VERSION     DAONVQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6753_length_8109_cov_4.391482"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2340
                     /locus_tag="PLD98_06540"
     CDS             <1..2340
                     /locus_tag="PLD98_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05938.1"
                     /translation="LQFQLTAAPKTSGTNSGYVEAAVSKVSVAKDNTVTIKPYKLKSG
                     TAYDPKFEGQTEITQKYNGKNVKTNIYVYNDYELIPLYNRLMALNYSYNDFAENFTYA
                     YSYNGETGTINSSAAWSNYNAALQEAAGYILQPKTLDSVASVVVKYKGAHDKLLRAYE
                     ILKQCPYKANSPYDVLKTYLEENEKTGFEAMGGADYMRYRWTKYSAAKLLADTIITQK
                     RLSNPENRLHLPGVNLTAAELKEIKNSSSQKYADVIRLYDDYAGSITGIASSIGNMLT
                     ESLILKPVNAADAKNMLERYSSRLIKIYTQPDKTYLSAAVDYAENTVLEAAAYAPKSY
                     AAFTEALNDAKAALLSNNITQTAINNARTELLRAFGSLILKEELVPEDIMQSLTDARE
                     RAYLALDARNSLRVNPDDFAGLLEAAGLVETVTGKNVQLYPKSVGALENAELKNTAST
                     IAEINRRIEKLDALIENIKGKQNANIILVSSADVDSALEANKTFTVEIKLGTDFKLKS
                     AVIPVYFNDSALEYKSASGSAAAVKNGNVITLTLSNGGDRRYANEVIAALTFKSTKAS
                     DGTKLIWVENKSANPIVLRSALSSDGQSIILDSAALTFKAANAVVPSYGGTIVRGGPR
                     NNKEAYSDGQGGYTAADFRIVSEISQAEALKYFGIPLNPTVQAYRSPIYELGTIVAFS
                     DYMKSANLTTLTLNNVDNKNVFAAPSQRRLYTDRAKPDGSGSYYWAAVIPKVPKEKWN
                     TTIYAVGYIKYYDANGNIKTVYTSGQHVSLSVESVALVK"
     gene            2566..2835
                     /locus_tag="PLD98_06545"
     CDS             2566..2835
                     /locus_tag="PLD98_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HQA05939.1"
                     /translation="MGIISWIIIGALAGWIASMITGNNRKMGAGKNILAGIAGAFIGG
                     FIMNLIGGTGITGFNLWSLLVATGGAVILLLIINAVSRTAKEQTN"
     gene            2955..4568
                     /locus_tag="PLD98_06550"
     CDS             2955..4568
                     /locus_tag="PLD98_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HQA05940.1"
                     /translation="MILEKLLNRFIRFQEKARETPAPKNGGNGDSQDNNLKGDSSDAL
                     LEKLSDNVNTIKELLGASQDVKYHNIKLRGPGAPEAMLIFIDGLVDEKMITEGILEPL
                     LTHEFNLAEGLAFGKLAQIIDKQILQMGDVEVSNKISKLISAILSGSSALIIDGVKEC
                     LLISTKGYPTRSITEPQTEVVVRGPREGFTENLRTNTAMIRRRIKSQDLRMEHMTVGK
                     KTETVICITYIKNVARQEIVDKLKERIEKIDTDSIIDSGYIEAFIEDSPFSLFETVGY
                     TEKPDVAAAKMLEGRIAILVDGSPFAITVPHLFVENFQTSEDYNTRFLYASVTRLMRF
                     VAYFLSVFTVPVFIALTTFHQELIPETLLFTIANAREGTPFPVFFEALIMVISFEVLK
                     EAGLRLPRPVGQAISIVGALIMGDAAIAAGLAGAPVVIAVAFTAVAGFIVPNQIESTS
                     ILRIIMMIIAATLGGYGLALGMLGLLIHLASLKSFGVDFLTTVIPSTDLKDSFVRAPL
                     WAMVRRPIALAGRNRIRMKPWLPQNSEKA"
     gene            4593..5933
                     /locus_tag="PLD98_06555"
     CDS             4593..5933
                     /locus_tag="PLD98_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HQA05941.1"
                     /translation="MSAKKITRRLLSVTAVVLIIAVLGGCWDKVELNAISIITGIGVD
                     AIPDNPDLMNVTIQSANVKALSGPASGGGGGDGEAPFIINEEQSKSISEAITQIGRRT
                     SRSPFTHHNQIIVVGKDYCKNGIQSVVDTFLREDEMRVETSIVVADGTAREMLSVKTE
                     IEKSASQWIPGMLASLNYNVGKVDVSVLDFANQILKPSGAAVIPLIKVEDTSLQKPML
                     KEKNAQQQEKKDEGEEKSGEEEKEEKKEAEEEKSEKKNGGEIETKTLSFVGFAVFKGP
                     YFVGEMGVAEMKGYRWLSGDVTSTRVSLSVGSGTANVNVTNAKFKKKVRLDESGKLIA
                     DFSIIADAAVLESIGIDDTDIKRYTKVLTEVMSEQIKKEIEDCLEKAKELNADVFGFL
                     NTLYEKHPKDYKRIEEKRDEVFKNMTLNTDIKITIQDTGKLKKSILEKWWNDEH"
     gene            5923..7089
                     /locus_tag="PLD98_06560"
     CDS             5923..7089
                     /locus_tag="PLD98_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HQA05942.1"
                     /translation="MSIDKSKITVTQFVFTIVCFIESSAVLSSFSVGISGKDSWFTMI
                     MGYLVFLILVFVISQIMRRYPDKTLIEINNEVFGNVLGRIISVFYIWFFVTLTSLNLL
                     DTQVFASSSIMTKTPPIVLMATFMVICVWALRNGISVITRYMTVIGLLSFAITVFAFI
                     IAFQKFHLSNLLPPMQQKPIVYVQASHIVSTIPFGEIIVFLMLTPNVSSSSKKYGRYI
                     LIGTLLGVISMLLVAIRDIGVLGETIKYFTVPSLETQRVSSISKIFSRLEVLYTIIFT
                     ALMYSKIVVLLYAATAAIAQFFKLKTYKNISLLIAALCIVYTLNLYDSGISHGLSGML
                     VVPFLWSFIEYILPILTLCVILIKSKFKKEGTENDAVRVNGKRGNLSLGPSLGN"
     gene            7321..7446
                     /locus_tag="PLD98_06565"
     CDS             7321..7446
                     /locus_tag="PLD98_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05943.1"
                     /translation="MAKKEKNKNKNKAENLNPKQEQQNKQEQNKEQNKKQNRREF"
     gene            complement(7501..>8109)
                     /locus_tag="PLD98_06570"
     CDS             complement(7501..>8109)
                     /locus_tag="PLD98_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HQA05944.1"
                     /translation="RIVKEVVSAVNIPVTVKIRAGWDKNNINAVEFAKVLEQNGAAAI
                     CVHGRTKDQMYAPPVNLDIIRDVKMSVSIPVIGNGDIETPLDAKKMLDYTGCDLVMIG
                     RGALGKPWLFKLINDYLEKGVIAEEPSIEERMEIMLFHIKRLCEHKGEYIGMLEARKH
                     AAWYTKSLRGAAAYRRELSALKSFEELKAISERIIKEQKELI"
BASE COUNT         2356 a         1855 c         1915 g         1983 t
ORIGIN      
        1 ctgcaattcc agctgacggc ggcccccaag acttcgggca cgaattcagg atatgttgag
       61 gctgccgtaa gcaaggtaag cgtcgcaaaa gacaatacgg ttacaataaa gccctataag
      121 ctgaagtcgg gtacagccta cgacccgaaa tttgagggtc agaccgaaat tactcaaaaa
      181 tacaacggca aaaatgttaa gacaaatatc tatgtttaca acgactatga gcttattccc
      241 ctttacaaca ggctcatggc gctgaattac agctataacg attttgccga gaatttcacc
      301 tacgcttact cttataacgg cgaaaccggc accataaaca gctccgccgc ttggagcaat
      361 tacaacgccg cattgcagga ggcagcggga tatatcctcc agcccaagac tttagatagt
      421 gtcgcgtctg ttgtcgtcaa gtacaagggc gcccatgaca agcttttgag ggcttacgag
      481 atattaaagc agtgccccta caaggcaaac agtccctatg atgttttaaa gacatatctt
      541 gaggaaaacg aaaaaacagg ctttgaggct atgggcggcg cggattacat gcgctacaga
      601 tggacaaagt acagcgccgc aaagctgctc gccgacacga taattaccca aaagagactc
      661 tccaatcccg aaaaccgcct gcaccttccc ggtgtaaatc tcaccgccgc agagctgaag
      721 gaaatcaaga acagctcctc tcaaaagtac gccgatgtta taaggctcta tgacgactat
      781 gcgggcagca ttacgggaat agcctcgtca atcggcaata tgctgactga gagcttaata
      841 cttaaacccg tcaacgcagc agacgcaaag aacatgctgg agaggtacag ctcaaggctt
      901 attaagattt acacccagcc cgacaagaca tacctcagcg ccgccgttga ctatgcggaa
      961 aacacagtgc ttgaggcagc ggcttacgct cccaagtcat atgcggcttt cacagaggcg
     1021 ctgaacgacg caaaagccgc gctcctgagc aataacataa cacaaaccgc aatcaacaac
     1081 gcaagaacag agcttctgag agcattcggc tcgcttatac ttaaagagga gcttgttccc
     1141 gaagacataa tgcagagcct aaccgacgcg agagagaggg catatctcgc ccttgacgca
     1201 aggaactccc tgagagtaaa tcccgatgat ttcgcaggtc ttcttgaggc tgcgggtctt
     1261 gttgagacgg taacgggcaa gaatgttcag ctctatccca aatccgtggg agcccttgag
     1321 aacgctgagc ttaaaaacac ggcctcaaca atcgctgaga taaacagaag aattgaaaaa
     1381 cttgacgctc tgattgaaaa cattaagggc aaacagaacg ctaacattat tctcgtttcc
     1441 tccgcggatg ttgacagcgc tcttgaggca aacaagacct ttacggttga gataaagctc
     1501 ggcaccgact ttaagctgaa gtccgcggta attcccgttt acttcaatga cagcgcgctg
     1561 gaatacaagt ccgcatcggg cagcgcggcg gctgttaaaa acggaaatgt aattactctg
     1621 actctttcaa acggtggcga cagaagatac gcgaacgagg tcatagctgc gctgaccttt
     1681 aagtcaacaa aggcaagcga cggcactaag ctgatttggg ttgagaataa gtccgcaaat
     1741 ccgattgtgc tccggtcggc tctctcaagc gacggtcaga gcataattct tgacagcgct
     1801 gcgcttacct tcaaagccgc aaacgctgtg gtgccttcat acggcggcac aatagtccgc
     1861 ggcggcccaa gaaacaataa ggaagcatac agcgacggac aaggcggata cacagcggcc
     1921 gatttcagaa tcgtttcaga aatcagccag gcagaggcgc tgaaatactt cggaataccg
     1981 ctgaatccca cggttcaggc atacagaagc ccgatttatg aactgggtac aatcgttgcc
     2041 ttctccgatt acatgaagtc cgcaaatctt acaactctca ctttaaacaa tgtggacaac
     2101 aagaatgtat tcgccgctcc ctcacagcgc aggctctata ccgacagggc aaagcccgac
     2161 ggcagcggca gctactactg ggcggcggtt attcccaagg ttccgaaaga aaagtggaat
     2221 accacaatat acgccgtggg ctacatcaag tactatgacg caaacggaaa tatcaaaacc
     2281 gtttacacaa gcggtcagca tgtatccctt tcagttgaat cggttgccct tgtaaaataa
     2341 gggcggcaaa agaaaaccgc ggctttggcc gcggtttttt atttttctct tttaatatta
     2401 aaattttaag gaaatattta agagccgaaa atgtgaataa gtaaatttta aaaattgata
     2461 atataatgat tgctgaactt ttataaaagt tttttcaaag tttttacttt gaaaatcgca
     2521 gtaatttact gcgacagcaa taaaatgcag agaaggaggc agtttatggg cattatcagc
     2581 tggattatca tcggcgcact tgcaggttgg atcgccagca tgattacagg caataacagg
     2641 aaaatgggcg ccggaaagaa tatacttgca ggcatagccg gtgcttttat cggcggattt
     2701 atcatgaacc tcatcggcgg aacgggcatt accggattta acttatggag tctgcttgta
     2761 gcaaccggcg gcgccgttat tctgcttttg ataatcaacg cggtaagcag gaccgcaaaa
     2821 gagcagacaa actaaagagc cccgtataag ctcaaggcct atggcaatta ttttgccata
     2881 ggccttaaac ttttgtatag gcaagaaaat tttgtatatc ctaaattaag aaattatgcg
     2941 cggatgtggt aaggatgata ctcgaaaagc tgcttaacag attcattcgc tttcaggaaa
     3001 aggcaaggga aacgccggct ccgaaaaacg gcggaaacgg cgactcccaa gacaataatc
     3061 taaagggcga ctcctccgac gcccttttgg aaaagctcag cgacaatgta aacaccatta
     3121 aagagctttt gggcgcgagt caagatgtaa agtatcacaa tattaagctc agaggccccg
     3181 gcgcacctga ggccatgctt atttttattg acggtcttgt ggacgaaaaa atgattacag
     3241 agggcatttt ggagcccctt ttgacccatg agttcaatct ggctgaaggc cttgcctttg
     3301 gaaagctggc tcagattatt gataagcaga tactccaaat gggcgatgtg gaagtcagca
     3361 acaagatatc caagctcatt tccgcgattt tgtcgggcag cagcgccctt attatcgacg
     3421 gcgtaaagga atgtctgctc ataagcacaa agggctaccc gacaaggagc attacagagc
     3481 ctcaaactga ggtggttgta agaggcccga gagaaggctt taccgaaaat ctcaggacaa
     3541 acaccgccat gataaggcgg agaatcaaga gccaggattt gagaatggag cacatgaccg
     3601 tcggcaaaaa gacggaaacg gttatctgca tcacctacat taaaaatgtg gcaagacaag
     3661 agattgtgga caaactcaaa gagagaatag aaaaaataga cactgactcg attatagatt
     3721 cgggctatat tgaggcgttt atagaggact ccccgttttc tttgtttgag acggtgggtt
     3781 atacggaaaa gccggatgtt gccgccgcca aaatgcttga gggcagaatc gccattctgg
     3841 ttgacggcag tccctttgcc ataaccgtgc ctcatctttt tgtggagaac ttccagacct
     3901 cggaggacta caacacaaga ttcctttacg cctcggttac aaggcttatg agatttgtgg
     3961 cgtacttcct ttcggtgttc accgttcccg tatttatagc tctaacaacc ttccatcagg
     4021 agcttatacc cgaaaccctg ctgttcacca tagcaaacgc aagagagggc acgcccttcc
     4081 ccgtgttctt tgaggcgctt ataatggtga tttcctttga ggtgcttaaa gaagcgggtc
     4141 tgagactccc ccgccccgtc ggtcaggcga taagcatagt gggcgccctc ataatgggcg
     4201 acgccgccat agcggcgggt cttgcgggcg cgcccgtggt catagcggtt gcctttaccg
     4261 ctgtcgccgg ctttatcgtg ccgaatcaaa tcgagtccac ctcaatactc agaatcataa
     4321 tgatgataat agcggcgaca ctcggcggct atggtctggc tttgggaatg ctgggacttt
     4381 taattcacct tgcgtcgcta aaatccttcg gcgtggattt tctcacaacg gtaattccct
     4441 caacggattt gaaagactca tttgtcaggg cgccgctttg ggcaatggtc agaaggccaa
     4501 ttgccttggc gggcaggaac agaatcagaa tgaagccgtg gctccctcaa aacagcgaaa
     4561 aagcataaaa ctatagttcg ggagagaata ggatgtcagc aaaaaaaata acccgcagac
     4621 ttttatctgt aaccgcggtt gttctcataa ttgctgttct cggcggctgc tgggacaagg
     4681 ttgagcttaa cgcaatttca atcattacgg gcatcggcgt tgacgctatc cccgacaatc
     4741 ccgaccttat gaatgtgacg attcagtccg ccaatgtaaa ggctctctca ggtcccgcaa
     4801 gcggcggtgg cggcggggac ggagaggctc cctttattat aaacgaggaa cagagcaaaa
     4861 gcatttccga agcgattacg caaatcggaa ggcgcacaag ccgctccccc tttacccatc
     4921 acaaccagat tatagttgtg ggcaaggact actgcaaaaa cggaattcaa agcgttgtgg
     4981 acaccttttt gcgagaggac gaaatgaggg ttgaaaccag cattgtggtt gccgacggta
     5041 ccgcaaggga gatgctgtcc gtaaagacag agattgaaaa gtcggcctcc caatggattc
     5101 cgggaatgct ggcgagtctt aactacaatg tgggcaaggt agatgtatcc gtgctggact
     5161 ttgcgaacca gattctgaaa ccatcaggcg cggcggtcat acccttaatc aaggttgagg
     5221 atacctccct tcaaaagccc atgctgaagg aaaagaacgc tcagcagcaa gagaaaaagg
     5281 acgagggaga agaaaaatcg ggcgaagagg aaaaggaaga gaaaaaggaa gccgaagaag
     5341 aaaagagcga aaaaaagaac ggcggagaaa tagaaacaaa gactttgagc tttgtaggct
     5401 ttgcggtttt taagggcccc tattttgtag gggagatggg cgttgccgaa atgaagggct
     5461 acaggtggct cagcggcgat gtcacatcaa cgagagtcag cctaagtgtc ggcagcggaa
     5521 ccgcaaatgt gaatgtaacc aacgcaaaat tcaagaaaaa ggttcgtctt gacgaaagcg
     5581 gcaaactgat tgcggacttt tcaattattg ccgacgccgc cgttctggaa tccataggaa
     5641 tcgacgacac cgacataaaa agatacacca aagttctaac cgaagttatg tccgagcaaa
     5701 tcaaaaaaga aattgaagac tgtcttgaga aggcaaaaga gcttaatgcc gatgtctttg
     5761 gctttttaaa tactctttat gaaaagcatc ccaaggatta taagcggatt gaggagaaaa
     5821 gggatgaggt ctttaagaac atgaccctta acaccgatat aaagataact attcaggaca
     5881 caggcaagct gaaaaaatcc atactcgaaa agtggtggaa cgatgagcat tgataaaagc
     5941 aaaattaccg tcacccaatt tgttttcacg atagtgtgct ttattgagtc ctcagcggtg
     6001 ctctcctcat tttccgtcgg catatccggc aaggattcat ggtttaccat gataatgggc
     6061 taccttgtct ttcttattct tgtgtttgtg atttcgcaga taatgagaag gtatcccgat
     6121 aagacgctta ttgagataaa caacgaagta ttcggaaatg tcctcggcag aatcatatct
     6181 gttttttata tatggttctt tgtgactctg acatccttaa atcttctgga cactcaggtt
     6241 ttcgcctcca gttccataat gacaaagact ccgccgattg tgctcatggc gacatttatg
     6301 gtcatttgcg tatgggcact gaggaacggt atcagcgtaa taacaaggta tatgaccgtt
     6361 atcggacttt tatcctttgc gattaccgta tttgctttta tcattgcctt tcagaagttc
     6421 catctttcaa atctgctgcc gcccatgcag caaaaaccta ttgtttatgt gcaggcatcg
     6481 catatagttt caaccattcc cttcggagag atcatagtct ttctgatgct taccccaaat
     6541 gtaagctcct catcaaaaaa atacggaaga tatattttaa taggcacgct gctgggcgta
     6601 atctcaatgc tcttagtggc aataagggac atcggagttt tgggagaaac aataaagtat
     6661 ttcaccgtgc cctcccttga gactcagaga gtatcttcga tttcaaaaat cttcagccgt
     6721 cttgaggtgc tttacacgat aatctttacg gctctgatgt acagcaagat tgtagtgctc
     6781 ttatatgcgg ccactgctgc gattgcccag ttctttaagc tgaaaaccta taagaacatc
     6841 tcccttctga tagcggcgct ttgcattgtt tatacactga atctgtacga ctcgggcatc
     6901 agtcacggtc tttcgggcat gctggtcgta cccttcttat ggagctttat tgagtatatc
     6961 ctccccattc tgaccctctg cgtaattctt atcaagagta agtttaaaaa ggagggaacg
     7021 gaaaatgatg ctgttcgcgt taatggcaag cgcggcaatc tgtctcttgg accttccctc
     7081 ggcaattaga acaaaagaga aaagggaaat cacaattgtg tcaatattgt cggctttggt
     7141 gataatcctt tgttttctcc aggctttcgg ggtgaacctc cccaaactca tgacgcttat
     7201 aaatgaattt tttatgtggc tgggaatcta ctataaaatg taataaagtt taaaacttct
     7261 gcataattgt ggaatttctg caaaagatat gaatgcaaat tttaagaaag gatgtttttg
     7321 atggcaaaga aagaaaaaaa taagaataaa aacaaggccg aaaacttaaa tcccaagcag
     7381 gagcagcaga acaaacagga gcagaacaaa gagcagaata aaaagcagaa cagaagagag
     7441 ttttaatatc ctgctttgag aaaataaaag aaaccgtcgg ggtatccgac ggttttcttt
     7501 ttatattagc tctttctgtt cctttattat ccgctctgag attgctttca gctcctcaaa
     7561 gctttttaag gcgctaagct ctctgcggta cgcagccgcc cctctgaggg attttgtgta
     7621 ccacgcggca tgctttcttg cctcaagcat accgatatac tcgcccttat gctcgcaaag
     7681 ccttttaatg tgaaagagca taatctccat tctctcctcg atgctaggct cctcagcgat
     7741 aactcccttt tcaaggtaat cgtttatgag cttaaacagc cagggtttgc ccaatgctcc
     7801 cctacctatc atcacaaggt cgcagcctgt gtagtccagc attttcttag cgtcaagggg
     7861 agtttcaata tcgccgttgc cgatgacggg tatggaaacg ctcatcttta catctcttat
     7921 aatatcgaga tttacgggcg gggcgtacat ctggtccttt gttctgccgt gaacgcatat
     7981 tgccgcggct ccgttttgct ctaatacttt agcgaactct accgcgttaa tattgttttt
     8041 gtcccagccc gccctgattt tgaccgtaac ggggatgttt acggcggaga ctacctcttt
     8101 tacaatcct
//
LOCUS       DAONVQ010000091         8021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_6835_length_8021_cov_4.402963, whole genome shotgun sequence.
ACCESSION   DAONVQ010000091 DAONVQ010000000
VERSION     DAONVQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6835_length_8021_cov_4.402963"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..338
                     /locus_tag="PLD98_06575"
     CDS             216..338
                     /locus_tag="PLD98_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05945.1"
                     /translation="MNSFSSLISKLIALIVTFGVLISFYTPVEPYNVKDEENLL"
     gene            335..1165
                     /locus_tag="PLD98_06580"
     CDS             335..1165
                     /locus_tag="PLD98_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA05946.1"
                     /translation="MSFVSISDTHIQSWTSLFEYKQAVYLARGLKELNAAAVKPDALA
                     ISGDITHHGDDAEYRALHAVFDFYPTPENLILAPGNHDFGNTSEGDYNEYRERFIDYY
                     KKITGRTIENPYYATVIKGYYFIVIASEAMAGTLQYFTQTQLDWLDEILTEAETVSPG
                     KPIFVFNHNPLKGTNNVDIKWPTGGTPGPQSDDIMEILQRHQNVFFITGHTHDKLDEG
                     RVMTIGNVHFVNTPCYHRNDDYGTGYVFEAYEDHVVLRARNLLKSEWRDFEYIFDLAS
                     "
     gene            1424..2290
                     /locus_tag="PLD98_06585"
     CDS             1424..2290
                     /locus_tag="PLD98_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQA05947.1"
                     /translation="MAKRKKNNTSVVLGAAVILFVAVLSISYALSSRRQPDMIEDTTN
                     TTTEEITSTEPVTQPATEEQTQTPAPTTEKAVAPSTEPQTKEVSADPLPVPAAVTITE
                     SNWNLVLVNLYYKMPDDYKVTLAPCIPGSDVKLDYRVAEYYEKMYNAAKADGITLTPY
                     SGYRSKDRQNSNFNRKIDSYISQGYSPAQAKVLAAQSIMPPGCSEHNLGLAMDIAGTD
                     ANFYKTAAYKWLMENAADYGFILRYPEDKVHITKVKYEPWHWRYVGVEAAKAMKASGQ
                     CLEEYLGKLPPS"
     gene            2383..3789
                     /gene="tig"
                     /locus_tag="PLD98_06590"
     CDS             2383..3789
                     /gene="tig"
                     /locus_tag="PLD98_06590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQA05948.1"
                     /translation="MSLKTAQKTDTNLYTLELTCDKERFGAALQKAYEEQKGNITVPG
                     FRKGKAPRAFIEKYYGESVFYDSAIDAVFPELYREAIEESKLETVGSPFDFDIKHVSK
                     EGFELSLKVYVKPEVEIGEYKGLSAVKKAVAVEEDEVEHRLHHMLEDNARILTVEDRP
                     AQNGDIVVIDFEGFTDGKAFEGGKAEKYELTLGSGQFIPGFEDQIIGKNAGESFDVNV
                     KFPEDYAKELAGKDAVFKVVLHEIKVKELPALDDEFAKDVSEFDTLDELKKSIEEDIK
                     ASKEQEAQRKFESDLLDKLVDSVKAEIPPVMVEAALDDIINDFSYRMQMQGLDMNTYL
                     QITGLDMKTMREGYRDRAERDVKLRLAVEKIAELENIEVTEDDIKEEYERLSKAYGVD
                     VESIKNAVDEDMLKKDIANKKAVQIVIDSAVVAEAEPEAEEAEEKPKKTAKKTTRKTT
                     TKKKAEEKADDAAEEKAE"
     gene            3867..4451
                     /gene="clpP"
                     /locus_tag="PLD98_06595"
     CDS             3867..4451
                     /gene="clpP"
                     /locus_tag="PLD98_06595"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HQA05949.1"
                     /translation="MPLVPTVIEQTNRGERAYDIYSRLLNDRIVVLSEEINDTTASLI
                     VAQLLFLEGQDSEKDISLYINSPGGSVSAGLAIYDTMQYIKCDVSTICLGMAASMGAF
                     LLAAGTKGKRYALPNSEVMIHQPLGGARGQATDIKIAADHILKTRDRLNRILAENTGQ
                     PLEVIARDTDRDFFMSAEEACAYGIIDKVITKRP"
     gene            4574..5878
                     /gene="clpX"
                     /locus_tag="PLD98_06600"
     CDS             4574..5878
                     /gene="clpX"
                     /locus_tag="PLD98_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HQA05950.1"
                     /translation="MARNDDGREREVRCSFCGKPQEHVERIIAGPNVFICNECVDLCS
                     SIVHNDEPLGRRRGSSSPKPEAKILPKPLEIKQKLDEYVIGQEAAKRTLSVAVYNHYK
                     RVFYKAKMDAEVQKSNVLLLGPTGVGKTMLAQTLAKILDVPFAIADATTLTEAGYVGE
                     DVENILLRLIQTADYDIEKAEKGIIYIDEIDKIARKSENASITRDVSGEGVQQALLKI
                     LEGTISNVPPQGGRKHPQQEFIQIDTTNILFICGGAFDGIEKIIEKRLGSSALGFEAN
                     IKSKKDLEFSQVITQVIPQDLIKFGLIPELVGRLSVVATLAALDRDALVRILKEPKNA
                     LVKQYKSLLKMDDVELEFDDAALYAMADLTLEKKTGARGLRSIMEQTLLPVMFEAPSD
                     HTIERIIISEATVRDGVPPLIERNPAKKKDDQAAGMNPRSAG"
     gene            complement(6212..7504)
                     /locus_tag="PLD98_06605"
     CDS             complement(6212..7504)
                     /locus_tag="PLD98_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05951.1"
                     /translation="MKKSYITTAIVAFVAVLLTTVFVIVKNNLPQPGATTDGPKVDEE
                     AYLQAVSFSGEIEAPLLKTDIKGVYFTITTDGAVKFYTLSEDTGLKEIQPDGKKEMSV
                     TVSNEGLRAQVYYIKRDGVLNGYGLFTADMNQHVYIYEYAFFKIVNVPVISSVPASYK
                     KDSSVLLLIDTDKEDFYKNDKVYEEQFLYDLEKGTNKSYLYIMNRAIDDRGAGRADYA
                     MMTDEVIKDKSGNSILFFSARHYHLYDTAKTLDIFKSGGSGNNTDNNRYIRDILGMYA
                     KNSDKGVIYLKHNKDKTVVQVMRYDGKNSEKLYEFPGKYDSYLRSGDYLFNTQNNTCY
                     SILTGKSYPLNVSDSGFKAEIFKISADGKKLFARGKTSSAPAIVLAEMGEEAFTSRIY
                     YNSAFKYLCALDITDDGRYLCTVSETADGGKYTLYILK"
     gene            complement(7592..>8021)
                     /locus_tag="PLD98_06610"
     CDS             complement(7592..>8021)
                     /locus_tag="PLD98_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05952.1"
                     /translation="IYTTADRNDIIRHMRVDTGEKTEGVIALSEPFDALYDFDGGFAF
                     KTERGGLYCISRWYALVNSFNLPQNAEELKVLSYGARLFAAVKTSGGVSLYSLDENGS
                     ASLILEGINAASAADCALAGDTLYYLSRASGRVEKAQLKN"
BASE COUNT         2258 a         1902 c         1948 g         1913 t
ORIGIN      
        1 tgactttcgc ctttatggta ctccctccat tgttcagttt ttagggtatg ttcccaagaa
       61 catgacagac cgtttacacg gtctgttttt ttgttgtatt ttttggaaat ttaatggatt
      121 gacctttgtt tgtcgaccgt gttattgtga aattagcaaa taaacccttt cttaaaatct
      181 ctaaacacgc caaaaaaaat ttagggggaa aattcatgaa cagcttttcg tcattaatct
      241 ctaagctcat cgctctgatt gtcacctttg gagtgttgat ttcgttctac actcccgtag
      301 agccttacaa tgtcaaagat gaggaaaacc tgctatgagc tttgtgtcga tttcggacac
      361 ccatatccag agctggacaa gtctttttga gtataagcaa gccgtttatc ttgccagagg
      421 gctaaaggag cttaacgcgg ctgcggtaaa gcccgatgcc ctcgccatat cgggcgacat
      481 cacccaccac ggcgatgatg ccgaataccg cgccttgcat gctgtatttg atttctatcc
      541 caccccagag aacctgattc tcgcgcccgg caaccatgat ttcggaaata ccagcgaggg
      601 cgactacaac gaatacaggg agcgtttcat tgactattac aagaagataa caggcagaac
      661 cattgagaat ccctattacg ccactgttat caaaggctac tactttatag ttatcgcctc
      721 tgaggctatg gccggcaccc tgcagtattt cactcagacc cagttggact ggcttgatga
      781 gattctgacc gaggctgaaa ccgtttctcc cggcaaaccg attttcgtgt ttaaccataa
      841 ccccctcaaa ggcacgaata atgtggacat caagtggccc acaggcggaa ctcccggccc
      901 gcagtcggac gatatcatgg aaattctgca gcggcatcaa aatgttttct tcatcactgg
      961 tcatacgcat gacaagcttg atgaaggccg tgtaatgaca attggcaatg tccactttgt
     1021 caatacaccc tgctatcaca gaaatgatga ctacggtacg ggatatgttt ttgaagccta
     1081 tgaggaccat gtggtgctca gagccagaaa cctgcttaaa tctgagtgga gagattttga
     1141 gtatattttt gatttggcaa gctgacacgc ttaaatataa aacaaaacac ccgaggcgtt
     1201 ggcgcaatgc ccgcttcggg tgctttgttt cttttgtgac cgagtaactg caattttaag
     1261 cgacgatgat atatagtaaa atcgctatca aagcgaaatc tttgttgttt tctgtaaaaa
     1321 cctcataagc cttgtaattg ttgacataat actaatcagg ttgtataatt ggtaggtatc
     1381 atatttattc aatttgcata ttaaattttg tcaggtgacc caaatggcta agagaaaaaa
     1441 gaacaacact tcggttgttc tcggagccgc tgtaattctg tttgttgcgg tcctttcaat
     1501 atcctatgcc ctgtcgtcaa ggaggcagcc ggatatgatt gaagatacaa cgaacaccac
     1561 aacggaagag ataacctcga ctgagccagt cactcagccc gcaaccgagg agcaaactca
     1621 aactcccgcg cctaccacag agaaggcagt agccccctca accgagccgc agaccaagga
     1681 agtctccgca gaccccctcc ccgttcccgc agcggtgacc attaccgaga gcaactggaa
     1741 tctggtgctt gtaaaccttt attataaaat gccagacgat tacaaggtaa ctctggcgcc
     1801 ttgcattccc ggctccgatg taaagctgga ctacagagtg gcggagtact acgaaaagat
     1861 gtataacgcc gcgaaggcgg acggcataac ccttaccccc tattcgggct acaggagcaa
     1921 ggaccgccag aactccaact tcaaccgcaa gattgattcc tatatatccc agggctattc
     1981 tcccgcccag gcaaaggtcc tggcagccca gagcatcatg cctcccggct gcagcgagca
     2041 caatttgggt ctcgccatgg atattgccgg tacggacgcg aacttttata aaactgccgc
     2101 atataagtgg ctaatggaga atgccgcgga ctacggtttt attctcagat acccggagga
     2161 taaggttcat atcacaaagg ttaaatacga gccgtggcac tggcgttatg tgggtgtcga
     2221 agcggcaaag gccatgaagg caagcggaca gtgtcttgaa gaatatctcg gcaagctgcc
     2281 gccttcataa attcaaaaaa actctggaaa aatcccctta tttttgatat aattaatcgg
     2341 tttattctgc aatactaatt gaaactcaat ggaggacaaa aaatgagctt aaaaacagca
     2401 cagaaaacag ataccaacct ttacaccctt gagcttacct gcgataagga gcgtttcggc
     2461 gccgcgctgc agaaggcata cgaagagcaa aagggcaata taaccgttcc cggcttccgc
     2521 aagggcaagg ctcccagagc tttcatcgag aagtactacg gcgagagcgt attctacgac
     2581 agcgcaatcg acgctgtttt ccccgagctt tacagagagg caatcgagga gtcaaagctg
     2641 gagacagtcg gttccccctt tgactttgac ataaagcatg tcagcaaaga aggctttgag
     2701 ctttcactca aggtttatgt aaagcctgag gtcgaaatcg gcgagtacaa gggcctttcc
     2761 gcggttaaga aggcagttgc ggtcgaagag gacgaggttg agcacagact ccaccacatg
     2821 cttgaggaca acgcaagaat cctcaccgtt gaggacagac ccgctcagaa cggcgacatc
     2881 gttgttatcg actttgaagg ctttaccgac ggcaaggctt tcgagggcgg caaggctgaa
     2941 aagtatgagc ttaccctcgg ctccggtcag ttcatccccg gcttcgagga ccagattatc
     3001 ggcaagaacg caggcgagtc ctttgatgtc aatgtaaaat tccccgagga ttacgcaaaa
     3061 gagcttgccg gcaaggacgc tgtattcaag gtagttctgc acgaaatcaa ggtaaaagag
     3121 ctgcccgccc ttgacgacga gttcgcaaag gatgtaagcg agtttgacac tcttgacgag
     3181 cttaaaaaga gcatagagga agatatcaag gcatcaaaag agcaggaggc tcagaggaag
     3241 tttgagtccg accttctcga caagctggtt gactccgtaa aggctgaaat cccgcccgtt
     3301 atggtagagg cagcccttga cgacatcatc aacgacttct cataccgtat gcagatgcag
     3361 ggtcttgaca tgaacaccta tctgcagatt acaggtcttg acatgaagac catgagagag
     3421 ggctacagag acagagcaga gcgcgatgta aagcttcgtc ttgctgttga gaagattgcg
     3481 gagcttgaga acatcgaggt taccgaggac gacattaagg aagagtacga gcgtctctcc
     3541 aaggcatacg gcgttgatgt agagtcaatc aagaacgctg ttgacgagga tatgcttaag
     3601 aaggatatag ccaacaagaa ggctgttcag atagtcatcg actccgcagt ggtcgcagag
     3661 gcagagccgg aagctgaaga agcagaggaa aagcccaaaa agaccgccaa gaagaccaca
     3721 agaaagacaa ccactaagaa aaaggcagaa gaaaaggctg acgacgcggc tgaagaaaaa
     3781 gccgaataaa ctataatttt accttttgtg attagaaatt tttattgtgc caataattat
     3841 cacagggtac agaaggagga agtaatatgc cgcttgttcc aaccgtaata gagcaaacaa
     3901 acagaggtga aagagcgtac gatatttact caaggctttt aaacgataga atcgttgttc
     3961 tttcagagga aatcaacgac accaccgcaa gccttatagt agctcagctc ctcttccttg
     4021 agggacagga ctccgaaaag gacatcagcc tctatatcaa cagccccggc ggctcggttt
     4081 ccgcaggcct ggctatctat gatacaatgc agtacatcaa gtgcgatgtt tcaaccatct
     4141 gcctcggcat ggcagccagc atgggagcat tcctgcttgc ggccggaacc aagggcaagc
     4201 gctatgccct ccccaacagc gaggttatga ttcaccagcc cctcggcggc gcaagaggcc
     4261 aggccacaga cataaagatc gctgccgacc atattcttaa aacaagggat aggctcaaca
     4321 gaattctcgc tgagaacaca ggtcagcccc ttgaagtcat cgcaagagat accgacaggg
     4381 atttcttcat gagcgccgaa gaggcatgcg cctacggcat aattgataaa gttattacaa
     4441 agaggccata acaaaggcaa agaaatataa aagttttttg gtagaataaa tgcgggataa
     4501 ggcgttcatc gcccgctccc gcatttaaac acagcaaagc aaaagcgtgc tttgagagca
     4561 ggagtgtgat tttttggcaa ggaacgacga cggcagggaa agggaagtaa gatgctcgtt
     4621 ctgcggcaag ccgcaggagc atgtggaaag aattatcgcg gggcccaatg tctttatctg
     4681 caacgagtgc gttgatttgt gcagctcaat cgttcacaac gacgaacctt taggcagaag
     4741 acgcggcagc agcagtccta agccggaggc taagattctg cccaaaccgc ttgaaatcaa
     4801 gcaaaagctg gacgaatatg taattgggca ggaagcagcg aaaagaactc tctctgtggc
     4861 ggtttacaac cactataaga gggtattcta caaggcaaag atggacgccg aggtccaaaa
     4921 gagcaatgta ctccttcttg gtcctaccgg cgtgggcaag actatgcttg cccagaccct
     4981 tgcgaagatt ttggatgtac cctttgccat tgccgacgcc acgactctga ccgaggcagg
     5041 ctatgtgggc gaggatgttg agaacattct cttaaggctc attcagaccg ccgattacga
     5101 tatagaaaag gccgaaaagg gtattatata tatcgatgaa atcgacaaga tagccagaaa
     5161 gtccgaaaac gcctcaatca caagggatgt aagcggcgag ggcgtacagc aggctctttt
     5221 gaaaatactc gagggcacca tctccaatgt ccccccgcag ggcggcagaa agcacccgca
     5281 gcaggagttt atccagatag acaccaccaa catcctgttc atctgcggcg gagcctttga
     5341 cggcatcgaa aagattattg aaaaaaggct cggcagctcg gctctcggct ttgaggctaa
     5401 tataaaatcc aagaaggacc ttgagttctc tcaggtcatt acgcaggtaa ttcctcagga
     5461 ccttataaag ttcggtctca tccccgagct tgtgggccgt ctgtcggtgg tcgcaaccct
     5521 tgccgccctt gacagagacg cgcttgtcag aatcctgaaa gagcccaaaa acgcccttgt
     5581 aaagcagtac aagagccttc ttaagatgga cgatgtggag ctggagtttg acgacgctgc
     5641 cctctacgcc atggcggatt tgacgctcga gaagaaaaca ggcgcaagag gtctcaggtc
     5701 cattatggag caaaccctcc tccccgtcat gtttgaggcc ccctctgacc acacgataga
     5761 gagaattata ataagcgaag caactgtaag ggacggagtg ccgccgctga ttgagcgaaa
     5821 ccccgcaaag aaaaaggacg accaagcggc cggcatgaac cccagaagcg caggataaat
     5881 taaagggtgc ggttttccgt acccttcttt ttatataaag ccaaacgccc ggagctgacc
     5941 tctccgagcg tttttatata ataagaaagg agaataatga ggaggaagat tatgccaggg
     6001 gattttcgcg ctttaggaaa gcatagaaca cacaagcaga aaccaaacgg aaaggaagtg
     6061 gaacacaaac tgaggaggta agttaccaaa aagagagaat tcattacaga agataattcg
     6121 cagaaacttt tacagcgccc actgcgcaaa aatcccctct gataatgagt atatcagagg
     6181 ggaagtacca ttaattggac tttatggata tttatttgag tatatacaag gtatatttgc
     6241 cgccgtctgc tgtttcggat acggtgcaaa ggtatctgcc gtcgtcggta atatcaaggg
     6301 cgcacaggta tttaaaggcg ctgttgtaat agattcttga agtgaaagcc tcctcgccca
     6361 tttcagctag cacaatcgcg ggagcggagc ttgtcttgcc ccttgcgaaa agctttttgc
     6421 cgtcggcgct tattttgaat atttcagcct taaagccgct gtcggaaaca ttcagcgggt
     6481 aagacttgcc cgtaagtatt gagtaacagg tgttgttctg ggtgttgaaa agataatctc
     6541 ccgaccttaa atagctgtca tactttccgg ggaactcata cagcttttcg ctgttctttc
     6601 cgtcatagcg cattacctga acgacggtct tgtccttgtt atgcttaagg taaataacgc
     6661 ccttgtcaga gttcttggcg tacattccga ggatgtccct tatatacctg ttgttgtcgg
     6721 tattgttgcc ggagccgccg cttttaaata tatccagggt ctttgcggtg tcataaaggt
     6781 gataatgcct tgcggagaag aaaagtatgc tgtttccgct cttatcctta atgacttcat
     6841 cggtcatcat ggcgtagtcc gcgcggcccg cgcctctgtc gtcaatagcc ctgttcataa
     6901 tgtaaaggta gctcttgttg gtgccctttt caaggtcgta aaggaactgc tcctcataaa
     6961 ccttgtcgtt cttgtaaaag tcctccttgt cggtgtctat aaggagaagt acggagctgt
     7021 cctttttgta ggaagcggga acggagctga taacggggac attcactatc ttaaagaagg
     7081 cgtattcgta gatataaaca tgctgattca tgtcggctgt gaaaagaccg tagccgttta
     7141 atacgccgtc ccttttaata tagtaaacct gagccctcag cccctcgttg ctgacggtga
     7201 cactcatttc cttttttccg tcgggctgaa tctctttaag tcctgtatct tctgaaaggg
     7261 tgtagaactt aacagcaccg tcagtagtga tggtaaagta gactccctta atgtccgtct
     7321 ttaaaagggg agcctcaatt tctccggaga agctaaccgc ctgaagataa gcctcctcgt
     7381 ccaccttcgg accgtcggtg gtggcgcctg gctggggcag attattcttt actataacaa
     7441 aaacggttgt caaaagaacc gcgacaaagg caactatggc tgtggtaata tagctctttt
     7501 tcaaggcctt gcctccaata ttagttttta caatgcaatg atacttaaaa tccgccccaa
     7561 agtcaataaa cgaagccgca gctcgcccga tttaattttt gagctgagcc ttttctaccc
     7621 tgccgcttgc gcgggaaaga taatagagag tatcacccgc caaagcgcag tcggcggcgg
     7681 acgcggcgtt tatgccttct aaaataagcg atgccgagcc gttttcgtcg agtgaataaa
     7741 gacttactcc gcccgaagtt tttaccgcgg caaagagccg agcgccgtat gagagaacct
     7801 tcaattcctc cgcgttttgg ggcagattga aggaattaac caaagcgtac catctgctta
     7861 tgcaataaag cccgcctctt tctgttttaa aagcaaatcc gccgtcaaaa tcgtacagag
     7921 cgtcaaacgg ctcgctgaga gcaataaccc cctcggtttt ctctcccgta tcaactctca
     7981 tatggcggat tatatcgttt ctgtctgcgg tggtgtaaat c
//
LOCUS       DAONVQ010000092         8000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_6858_length_8000_cov_4.478792, whole genome shotgun sequence.
ACCESSION   DAONVQ010000092 DAONVQ010000000
VERSION     DAONVQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6858_length_8000_cov_4.478792"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLD98_06615"
     CDS             complement(<1..262)
                     /locus_tag="PLD98_06615"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQA05953.1"
                     /translation="MEKRRNVFRYISFIMSFIMIFSIFSAGELTAKADEFTFQPGMYA
                     VNVNTSLRLRSEPGTNGAILDSLPNGTVVYVTEVNGSWGKITY"
     gene            complement(525..1115)
                     /gene="lexA"
                     /locus_tag="PLD98_06620"
     CDS             complement(525..1115)
                     /gene="lexA"
                     /locus_tag="PLD98_06620"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HQA05954.1"
                     /translation="MKPLNDTQKRIYEYLAERSQDGVPPSIREICKAVGLRSTSSVQA
                     NLDALEEAGYISRDPLLKRSIRVLGQPDNVTNVPLVGTVTAGMPILAVEHIEGYIPYS
                     GKVSRDKPLFALRVRGDSMINAGILDGDIVISEKTPDAENGDIVVAMLEDEATVKRFY
                     KDADCFRLQPENDAYEPILSTEVTILGKVVAVLRYY"
     gene            complement(1290..2525)
                     /locus_tag="PLD98_06625"
     CDS             complement(1290..2525)
                     /locus_tag="PLD98_06625"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQA05955.1"
                     /translation="MKTSTELRKDTLSYIKDGIAYVCKNFKNRSPGSESEKKAQEYFA
                     KELANYADEVKSETFTLHPKGFMGFIPIVSVLMIISVVLFFVQKYVRNPVFPYISAVL
                     TLISIAMFLFEFLFYREFIDFLFPKAESVNVYAVRKAKNEPKRRIIFGGHADAAHEWT
                     YSLHGQIKTLLPVMGGGILSTFGLMFVFLIYIIKGTPDLNAGAWNVISWLSLVTIVFY
                     IALMFFINWRVIVDGANDNLSACFVSIGILREMAKNDFRYDDTEVGVIITGSEEAGLR
                     GALAFGKKHAKELNEIETIIIPLETLRETKHLAIYALDQTGTVRNDKKVGELLRRAGK
                     NVGIDLPYADIYPGSTDAAGFTRTGIKSCGLGGVDHNPRTYYHTRHDTYTNIGLDCME
                     KTLEICIEAANIFDKEGLG"
     gene            complement(2630..3343)
                     /locus_tag="PLD98_06630"
     CDS             complement(2630..3343)
                     /locus_tag="PLD98_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05956.1"
                     /translation="MALLKVSNLEVSYGVIRAIKGISFEVNEGEIIALIGANGAGKTT
                     ILNTITGLVPAKSGTVEFDGKNITKTAAHKIVRMGMAHVPEGRRIFQNLTVYDNLKLG
                     AFTRDDKKEFKENLERVYASFPRLEERKNQIAGTLSGGEQQMLAIGRGLMSNPKLMLL
                     DEPSMGLSPIFVAEIFEIIQTINKNGTTVLLVEQNAKKSLSISNRAYVLETGKITIAD
                     TSENLMNNEAVKKAYLGED"
     gene            complement(3356..4114)
                     /locus_tag="PLD98_06635"
     CDS             complement(3356..4114)
                     /locus_tag="PLD98_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA05957.1"
                     /translation="MIMLKTVDLGISFGGLNAVNAFNIEVEEGELYGLIGPNGAGKTT
                     VFNLLTGVYKPTTGSFSLAGVDLTGKSPTEICNHGIARTFQNIRLFKNMTVLDNVKVA
                     LHQRMKYSLASSFLRLPNYFSMEKKMNEEALRLLKVFDLDNVKDYNAASLPYGKQRKL
                     EIARAMATNPKLLLLDEPAAGMNPNETAELMETIRFVRGEFNMTILLIEHDMRLVAGI
                     CERITVLNFGTELASGETSKVLKDPQVITAYLGE"
     gene            complement(4101..5093)
                     /locus_tag="PLD98_06640"
     CDS             complement(4101..5093)
                     /locus_tag="PLD98_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQA05958.1"
                     /translation="MKKLLKSKSLIFTSVLIVLYILISVLMFEGKFTRSQQAILLKMC
                     SSIILAMSLNLVVGSLGDLSLGHAGFFCVGAYAGGYFAVNANLPLPLVFVIALIIGGI
                     TGALFGFFISSTILRLRGDYLAIVTLAYGELIRSFVKIIPALGGTRGLIGVPSLGARG
                     KQFLYMFAVVIVSFILISNFTRSRHGRAINSIRDNAIAAQSIGININRYKVMTFTISA
                     FFAGMAGVVFAFFKSNLEPSDFTYNLSIEALLMVVFGGMGNLFGSVVAAAVITALPEI
                     LRDAEEYRLLIYAISLILLMIFTSNPKLVELRKRIMPKTLFAGRRKEGATDDNA"
     gene            complement(5107..5994)
                     /locus_tag="PLD98_06645"
     CDS             complement(5107..5994)
                     /locus_tag="PLD98_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQA05959.1"
                     /translation="MQVFFNFVMDGLNLGSVYALVALGYSMVYGIAKMLNFAHGDIIM
                     VGGYAIYVMFLQKDLGIAAAVVASVIVCSLLGIIIEFLAYRPLRNASPLSVLITAIGV
                     SYLLQNIALLTFTSELKLISGFIKLPTFYVGPVRIDGVSILIWITTLAIMVALNYFIQ
                     NTKTGKAMLAVSEDRGAAQLMGIDVNKTITITFFIGSALAGLASVLYIAKYQKIEPYT
                     GSVFGIKAFSAAVFGGIGSIPGVALGGILIGLIEALAIGYLPDTLRPASDGVAFAILI
                     IMLVLKPSGILGKTKIEKV"
     gene            complement(6792..>8000)
                     /locus_tag="PLD98_06650"
     CDS             complement(6792..>8000)
                     /locus_tag="PLD98_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA05960.1"
                     /translation="VILKDGITFSDSSLLTSADVVYSFHQARLSPSYKTQLSAFSSCE
                     AAGRYEVRFALKQPTLSPLSLLDFPIVKQNTADQSEQVPKGSGRYIYRAENGEYLLEP
                     NPSYRENNDIARTIRLIPIGESVSPVFILETGELDACFDDLSSGAVRRADAAQTGAVL
                     NNLVYLGVNSKNYVLSDKAVRNALSRTILRSDISTRVYSGNALPAQAPVNPAKQSYSY
                     FADNIKEGIEVWEELGYNQFTSGGVRRGGRGVLSFTLICPSENQFKVQLAKSVAGSLK
                     EAGIKITVRELTTQQYKEALNAGNYDLYIGETRLLNNMDLSEFFKADGALSFGIDPES
                     PIAKAYFDYVGGTISESVFCRQFVEESPFIPLVFRMGVLYYSRGIDKEVKPVGASLNV
                     YANIHEWNAD"
BASE COUNT         1951 a         1900 c         1982 g         2167 t
ORIGIN      
        1 tgtaggttat cttgccccag gagccgttaa cctccgtgac atacacaacg gtgccgttcg
       61 gcagagagtc caaaattgcc ccgtttgtgc cgggctctga gcgaagtctc agacttgtgt
      121 ttacatttac cgcatacata cccggctgga aggtaaactc gtcagccttt gcggtgagtt
      181 ctcctgccga aaaaattgaa aatatcatta taaagctcat tataaagctt atgtatctaa
      241 aaacattcct tcttttctcc atattttccc tcacttttat ctgcatcgct gtttgttccg
      301 cttcttacag ccccaaatgg ggcacggttt ggtttcttcc tttggcccct ccccccatcc
      361 ttgggtcggg gcacgattta attatggact gaaaaaagtt ttttttcaac agatttctgc
      421 atttttcccg taaagtattg ttttccaaaa taatttgcaa agaatagcaa aataaaaggc
      481 ggcaattttc atgccgccta aatggatttt gagtttaata agtatcaata ataccttaat
      541 accgccacga ctttgccgag gattgtgacc tctgtggaca aaatcggctc gtaggcgtcg
      601 ttttcgggct ggagcctgaa gcagtctgcg tccttataaa agcgttttac ggtcgcctca
      661 tcctccagca tggcaacgac gatatcgccg ttttcggcgt cgggggtttt ttcggagatg
      721 acaatgtccc cgtcaagaat gcccgcgtta atcatactgt cgccgcggac tctgagagca
      781 aacaggggtt tgtcgcggga gaccttgccc gaatagggta tatagccctc tatatgttca
      841 accgccaaaa tgggcatacc tgcggtaacg gtacccacca gtggcacatt cgtcacattg
      901 tccggctgac cgaggaccct tatcgacctt ttgagcagcg ggtcgcggga gatatatccc
      961 gcctcctcaa gggcgtcaag gtttgcctgc accgacgatg tggagcgcag tcccaccgcc
     1021 ttgcagattt cccttattga agggggtacc ccgtcctggg agcgttcggc aagatattca
     1081 tatatccttt tttgagtgtc gttaaggggt ttcaccctat ccccgtcctt tctgcagaag
     1141 ggcgttctaa atctgaaaaa actgtaaagt tttaggaaaa atactctgtt tgcgataagt
     1201 ataacacaag tttttcaaaa aagcaaacat ttgtttgcac aaaatgtgaa aaaaacggca
     1261 gccgaagctg ccgtcctgag tttaagttat tatccgagac cctctttgtc aaagatgttt
     1321 gccgcctcta tgcagatttc aagagtcttt tccatgcagt caagaccgat gttggtatat
     1381 gtgtcgtggc gggtgtggta gtaggttctg ggattgtggt caacgccgcc cagaccgcag
     1441 gacttgattc ccgtgcgggt aaagcccgct gcgtcggttg agccggggta aatatcggca
     1501 tagggaaggt cgatgcctac attcttgccc gctctcctta aaagctctcc aacctttttg
     1561 tcgttgcgca cggtgcccgt ctggtcaagg gcgtagatgg caaggtgctt tgtctctctc
     1621 agagtttcaa gggggataat gattgtttcg atttcgttaa gctccttggc gtgttttttg
     1681 ccgaatgcca gagcgcctct gagacctgcc tcctcggagc ctgtgatgat tacgcccacc
     1741 tctgtgtcgt catagcggaa atcgttcttt gccatctctc ttaaaatgcc gatggataca
     1801 aagcaggcgg aaaggttgtc gttcgcgccg tctactatta ccctccagtt aatgaagaac
     1861 ataagggcaa tatagaaaac gattgtaacg agggagagcc atgaaatcac attccatgcg
     1921 cccgcgttca ggtcgggagt gcctttgatt atatagataa ggaaaacaaa cataaggcca
     1981 aaggtgctga ggatgccgcc gcccattacg ggtaaaaggg tcttgatttg accgtggaga
     2041 gaataggtcc actcatgggc cgcgtcggcg tgtccgccga agattatcct tctcttgggc
     2101 tcgttcttag cctttcttac ggcatagaca tttaccgatt ctgcctttgg gaaaaggaag
     2161 tctataaact cgcggtagaa aaggaactca aacaagaaca tggcgattga gataagggtt
     2221 aaaaccgctg atatgtaggg gaatacgggg ttccggacat atttttgcac aaagaacaat
     2281 actacgctga ttatcataag aaccgatact atggggataa aacccataaa tcccttggga
     2341 tgaagggtaa aggtttctga ttttacttcg tccgcatagt ttgccagttc cttcgcgaaa
     2401 tactcctgtg cttttttctc cgactcggag ccgggggaac ggtttttgaa attcttgcag
     2461 acataggcaa ttccgtcttt aatgtagctt agggtatcct tgcgcaattc ggttgatgtt
     2521 ttcatgcaga ctgtcctctc cttcaaaatt tagttaaatt ttaccagcaa aatgtgaaaa
     2581 aaaatttaac gacgcaaaaa aagacgcggg aaaattccgc gtctttttat tagtcctcgc
     2641 ccaaataggc ttttttgaca gcctcgttgt tcataaggtt ctcggaagta tcggcaatgg
     2701 taatcttgcc ggtttcgaga acataggctc tgtttgaaat ggaaagggac tttttagcgt
     2761 tctgctcaac caacaggacg gttgtgccgt ttttgtttat tgtctgaata atctcgaaaa
     2821 tctcggcgac gaaaatcgga gaaagtccca ttgagggctc atcaaggagc atcagcttgg
     2881 gattggacat aagtcccctg ccgatggcaa gcatctgctg ctcgccgccg ctgagagttc
     2941 ccgctatctg attctttctt tcctcaagtc gagggaagct ggcgtaaact ctttcgagat
     3001 tttccttaaa ctcctttttg tcgtcccttg tgaaggcgcc gagtttcaga ttgtcgtaga
     3061 cggtcaggtt ctggaaaatg cgcctgccct ccgggacatg agccatgccc atccgcacga
     3121 ttttgtgggc ggcggtcttg gttatgttct ttccgtcaaa ctcgacggtg ccgctctttg
     3181 ccgggacgag tcccgttatt gtattgagaa tggttgtctt gccggcgccg tttgcgccta
     3241 tgagggctat aatctcgccc tcgtttacct caaaggatat gcccttgatg gctctgatga
     3301 cgccgtagct tacctctaaa ttgcttacct ttaataatgc cactgagctt cccccttact
     3361 cgccgagata ggctgtgatg acctgcgggt cctttagcac tttcgatgtt tcgccgctgg
     3421 caagctcggt gccgaagttg agaacggtaa tcctttcgca gatgcccgcg acaaggcgca
     3481 tatcgtgctc tataagcagg attgtcatat tgaattcgcc gcggacgaat ctgattgttt
     3541 ccataagctc cgcggtttcg ttgggattca tgcctgcggc gggctcgtca agaagaagga
     3601 gtttgggatt tgtagccatt gcccttgcga tttcaagctt tctctgcttg ccgtagggaa
     3661 ggcttgccgc attgtagtcc tttacattgt caaggtcgaa aaccttgaga agccttaaag
     3721 cctcctcgtt catcttcttc tccatggaga agtaattggg cagccttaaa aagctggagg
     3781 caaggctgta cttcattctc tgatgaaggg cgaccttaac attgtcaaga acggtcatat
     3841 ttttaaagag ccttatgttc tggaaggttc ttgctatgcc gtggttgcag atttcggtag
     3901 gcgatttgcc cgtaaggtca acgcccgcaa ggctgaaact gcccgttgtg ggcttgtaaa
     3961 cgcctgtgag aaggttgaac accgtggttt tgccggctcc gttgggtccg ataagaccgt
     4021 aaagctcgcc ttcttctact tctatgttaa atgcgttaac cgcattaagg cctccgaagg
     4081 agatacccaa atcaaccgtt ttaagcatta tcatctgttg ctccctcctt acgacgaccg
     4141 gcgaacagtg tttttggcat aattctcttg cgcaattcca cgagcttcgg gttggatgtg
     4201 aatatcatga gaagtatcag ggatattgcg taaatcagga gtctgtactc ctcggcgtct
     4261 ctcagtatct cgggcagagc cgtaattaca gccgccgcga ctaccgagcc gaagagattg
     4321 cccatgccac cgaagaccac catcaaaagc gcctcaatcg aaaggttgta ggtaaagtcc
     4381 gagggctcca aattgctctt gaagaaagcg aacaccacgc ctgccattcc cgcaaagaat
     4441 gcggagattg tgaaggtcat taccttatag cggtttatgt ttatgcctat tgactgagcg
     4501 gctattgcgt tgtctctgat ggagtttatt gctctgccgt gacgggatct ggtaaagttt
     4561 gaaatcagta taaagcttac tataacaacc gcaaacatgt ataggaactg cttgcctctt
     4621 gctcccaggc tggggacgcc gataagtccc cttgttccgc cgagagcggg aattatcttt
     4681 acaaatgaac ggatcagctc accgtaggca agggttacta tcgccagata gtcgcctctg
     4741 agccttaaaa tggtggagct tatgaagaat ccgaaaaggg ctccggtaat gccgcctata
     4801 ataagggcga ttacgaatac caagggcagc ggcagattcg cgtttacggc gaagtatccg
     4861 ccggcgtatg ctcccacgca gaagaagccc gcgtgaccca gcgacaggtc gccgagggaa
     4921 cctactacga gattcagcga catggcaagt atgatgctgg agcacatttt gagaaggatt
     4981 gcctgctggc tccttgtgaa cttgccttca aacatcagaa cggaaattaa tatgtaaagt
     5041 acgattaata ccgaagtgaa gatgagtgac tttgatttaa gcagtttttt catcccaaag
     5101 ccccccttaa accttttcaa tcttggtctt gcccaaaatg ccggagggct ttaacacaag
     5161 cataatgatg agtattgcga acgcgacgcc gtccgacgcg ggacgaaggg tatcgggaag
     5221 atagccgata gccagcgcct cgataagtcc gataagtatt ccgccgaggg ctacgccggg
     5281 aatgctgccg atgccgccga atacggccgc ggagaatgcc tttatgccga ataccgagcc
     5341 tgtataaggc tcaatctttt gatactttgc tatgtaaagt acgctggcga gtcccgcgag
     5401 agcggagcct ataaagaagg tgatggtaat tgttttgttt acatcaatgc ccatgagctg
     5461 agccgctccc ctgtcctcag acaccgcgag catagccttt cctgtcttgg tgttctgtat
     5521 gaagtagtta agcgcaacca ttattgcaag agttgtaatc caaataagta tcgatacgcc
     5581 atcaatcctc acaggaccca cataaaaggt ggggagctta ataaagccgg agatgagctt
     5641 aagctctgat gtaaaggtta aaagcgcaat attctggagc aggtagctga cgccgatagc
     5701 tgtaataagg acggaaagag gagatgcgtt tctaaggggg cgataagcca aaaactcaat
     5761 gataatgccc agcaaagagc agacgattac cgaagcgaca accgccgcgg cgatgcccaa
     5821 atccttttgc aggaacatta cataaattgc gtagccgccc accatgatta tgtcgccgtg
     5881 ggcgaagtta agcattttgg cgatgccgta aaccattgag tagcccagcg cgaccagcgc
     5941 gtacacactg cccaggttca gtccgtccat tacgaagttg aaaaaaacct gcataaagat
     6001 tatcactcct aagtttcgag cgcagacaaa cagtcttttt attaaaaagc aaaaactttg
     6061 aatgagtctg ccgatttcca gaatttgttt actttggttt tgccttaaag cgttgtaacg
     6121 ctaaagtaat aaaggttgca ataacttaca ttatagtatc aaaattcacc gaattcaatc
     6181 gaattaaaaa attttctgat attttacatt ataattcgct aaaatacaat aaatttgtcg
     6241 ggttgatttt tgcctttttc cgccgcctgc ggggcttggc ggattcgctg ctgagtattt
     6301 cgccgccgct tgaaccgccg cgggcgggca tttcgcttgc cgccgaataa ggggatgctt
     6361 tgccgccgtt cccttgcggg gtcggggcgc tgcggcacct gaccgccaag ggcggttttt
     6421 ttcttgccgc cgtacccccc cctgacgctg atttgccacc agcggcataa gtgattttcc
     6481 gcggagcgag aaaagtcttg ttaattgaaa atatattttt atttgtcgcc tttattcaag
     6541 aattttatgg gcaattcggg gagagtgtgt tttaaagccc aaaaagcgca aaaatttgga
     6601 ttttcattta tatataagct ctaaatccat atgcgctgta agtttatttt taggcgattt
     6661 atacaatttc acacccacaa aaaatgcaca aaacagcacc ggggtatctg agggatatcc
     6721 cagtgcatta atatctcagt aattatatca cttgtcggct ctgattacaa ggctttttta
     6781 ttatttccgc gctagtccgc gttccactcg tggatattgg cgtaaacatt gagacttgcg
     6841 cccacgggct ttacttcctt gtcaatgccg cgggagtagt agaggacgcc cattctgaaa
     6901 acaaggggta taaaggggct ttcttccacg aactgacggc agaacaccga ctcggagatt
     6961 gtgccgccca catagtcgaa atacgccttg gctatggggg attcggggtc aatgccaaag
     7021 gacaaagctc cgtcggcttt aaagaactcg gacaagtcca tattgtttaa aagccttgtt
     7081 tcaccgatat acaaatcgta attgccggcg tttaaagcct ctttgtactg ctgtgtggtc
     7141 agttctctga cggtgatttt gattcccgcc tctttaaggc tgcccgcaac agactttgca
     7201 agctgaactt taaactgatt ctccgacgga cagattaaag tgaaacttaa aactcccctg
     7261 ccgccccttc ttacgccgcc ggaggtgaac tgattgtagc ccagctcctc ccaaacctcg
     7321 atgccctcct taatgttgtc ggcaaagtag gagtagcttt gctttgcggg gttgacggga
     7381 gcctgagcgg gcagggcgtt gcccgaatac actcttgtgc tgatgtccga gcggagaatg
     7441 gtgcgggaaa gggcgttcct gaccgccttg tcggagagca catagttttt gctgtttacg
     7501 cccaaataca caaggttatt gagaaccgcg cccgtctgag cggcgtcggc tctgcggacg
     7561 gctcctgagg acaaatcgtc aaagcaggcg tccagctctc ccgtttcgag tatgaatacg
     7621 ggggacacgc tctctcctat gggtatgagc cttatagtcc ttgcgatgtc gttgttctcc
     7681 ctgtaggagg ggttcggctc caaaaggtac tcgccgtttt cggctctgta tatgtatctt
     7741 ccgctgcctt taggcacctg ttcgctttgg tcggcggtgt tttgttttac tatcgggaaa
     7801 tcaagcaggc taagcggtga gagcgtgggc tgtttaagcg caaaacgcac ttcgtacctt
     7861 cccgcagcct cgcaggagct gaaggccgaa agctgagttt tgtacgaggg cgagagcctt
     7921 gcctgatgga aggagtaaac cacatccgcg cttgtaagca ggcttgagtc gctgaaggtt
     7981 atgccatctt ttaaaatgac
//
LOCUS       DAONVQ010000093         7987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_6870_length_7987_cov_4.908220, whole genome shotgun sequence.
ACCESSION   DAONVQ010000093 DAONVQ010000000
VERSION     DAONVQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6870_length_7987_cov_4.908220"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLD98_06655"
     CDS             complement(<1..447)
                     /locus_tag="PLD98_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA05961.1"
                     /translation="MENVSNERYMTTRRERVSYWSYFVGQNIFYTIVSTYLLTYLAMI
                     GVSMTKISAVVLIVKIWDAFNDPIFGWIFDKVKFKSGQKSLPWLKISLLAIPLTTIFM
                     FAIPSGLDEGMKLFWFGAAYVLWDTAYTICDVPIYSMVTTMTDNLHE"
     gene            complement(461..2908)
                     /locus_tag="PLD98_06660"
     CDS             complement(461..2908)
                     /locus_tag="PLD98_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HQA05962.1"
                     /translation="MSIKEDIIKFFVKTYSRLTGVGQMQQVGTGEYKITEGAPELIRA
                     AAAEGAVLLENRVLPFKRGSTVSVFGRTQLDWFYTGYGSGGDVIRPYQINLAEGIKNC
                     PKLKINESLLSIYEKWVEENPVDHGAWAFWPRSYPEMPLLSAVVADAAKNSDHAVVVI
                     GRSSGEDRENVLEKGSYYLTDDEREMLNKVTEHFNDVVVLLNIGSIMDFSWIEEYREK
                     LGAVMIVWQGGMESGNAVADLLCGDAVPSGRLTETIARDYNDYPSSSNFGNKEYNEYQ
                     EDIYVGYRWFETFNKDAVLYPFGYGLSYTEFKVVKHAVKSFPDGIKIAAAVKNVGEIF
                     AGKHVLCAYIEKPCGVLGNPSRELADFAKTGAIFPGQTENVLLEIPTTRLASYDSSGA
                     TGNKSCYVIEKGVYRIYLGSDVRSAEKIFEFTIKENIVVEKLKEAAAPKYPFSVYKAV
                     EDENGNRRPEMDLAPASTVNLRQIILDNLPEEKPMTGDMGFKLKDVAKGKVSLERFTA
                     QLSLEELEAITRGDYIMNSPLGPEGNAGVFGGVLQSLRDKGVTPVTTTDGPSGIRLKQ
                     SCSLIPIGTALASTFNKDLVKELYHKVGLEMKEKDSDVLLAPGMNIKRNVLCGRNFEY
                     FSEDPILSGKIAAAAVAGLQSAGVSACPKHFACNNQEYNRLHNDSRVSERALREIYLK
                     GFEICVKEAKPHAIMTSYNKINGVWGHYHYELCERILREEWGFDGLVMTDWWMRSSKS
                     PEFPEIRDNAYRVRSGVDLLMPGGKRIGPKKPDGTLLKTYGRTHGITLGEMQRCAMHV
                     LTAVMRSNTFRQSLNKD"
     gene            complement(3087..4538)
                     /locus_tag="PLD98_06665"
     CDS             complement(3087..4538)
                     /locus_tag="PLD98_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HQA05963.1"
                     /translation="MNYRKVLKSIGSLLIIEAACMVPSMLVSLFYKEKAVYSFAISIV
                     ITAIAGFILYRLKAPNQNIYARDGFAITALGWLSVTVFGALPFLISGSIPSVIDAFFE
                     TASGFTTTGASILREVESLPKGILFWRSFTHWMGGMGVLVLMIAVLPSTKANAVYIMK
                     AEASGPSPDKLVPKIRQTAEILYIIYIAITFIIIVLLLLGGMPLFDSIAHAMGTVSTG
                     GFSVRNASIAAYNSVYIETVIILSMLISGINFTLYYFLLKKNVKGFLKNEELLYYIGV
                     IAVAIVLIVANIYGKVYSTIGESLRYASFQVSSLITTTGYVTADFNQWPTLSQMVLLL
                     IMFIGGCAGSTAGGIKCIRILLLSKMFKREITKLNHPRAVKTVRINGHAVDEEILSGI
                     KMFFFVYVAIFAAGVLVISLEGFDLPTTISAVVATLNDIGPGLGLVGPVGNYADFSVL
                     SKVVLSICMMIGRLEVYPILVLLFPSFWKKNTI"
     gene            complement(4554..5906)
                     /gene="trkA"
                     /locus_tag="PLD98_06670"
     CDS             complement(4554..5906)
                     /gene="trkA"
                     /locus_tag="PLD98_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004442143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HQA05964.1"
                     /translation="MKIIIIGDGKLGYSLADSLSKENNDVTIIDKDPQALRKAADYLD
                     VMCIKGNGLSSSTLLEAGIKEADLLIAATTSDEINMVCCLTAKKLGAAHTIARIRDPE
                     YANELTQLKADLDLDMIINPEHAVAGEIARLIEFPPAANVEVFKKGRVVLLEMRLTEN
                     MSIVNKSLKDITEKGIKGILIGGVLRGDDVIIPKGDFVLLPGDKIFIVGRNSRIVQFC
                     KHIGVYMPKIKSVMIMGGGRIAYYLAKYLQEMEIKVKIIEKNKDRCLELSELLPKALI
                     INGDGSDETILNSENLDSMGAFVAITDRDEDNIIAALLAKQYGVKKSIAKISRENFFD
                     AIMNLGIDNIVSPKQITANFILSYVRGLKNAMGNPVDVLYKIMENKAEAIEFTAGKTT
                     KFLDVPLKNLKLKEGILIAAIIRKNDIIIPHGSDVIKEKDNVILFVKDKELTDLNDIV
                     "
     gene            6221..6460
                     /locus_tag="PLD98_06675"
     CDS             6221..6460
                     /locus_tag="PLD98_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05965.1"
                     /translation="MGAYTGALEFFGSPLDIVAKEFNYDEDNILILRGDIAALHSKYP
                     ETYRNLESCTHHRDNRTALQYAQDLVASWLIEDYF"
     gene            6493..6891
                     /locus_tag="PLD98_06680"
     CDS             6493..6891
                     /locus_tag="PLD98_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05966.1"
                     /translation="MHGVDRTRRILASSQTSTVSDFLITRDGVEIKLELMNDYTGYWR
                     RYNVLHLRDFKFNQLKKSSAVLICISVPTGEFALYDFNEDIRARYMPSHRPYGGKPAY
                     EIGIQNEMIEKIGENNIQMAIIEIFDRRRA"
     gene            complement(7424..7894)
                     /locus_tag="PLD98_06685"
     CDS             complement(7424..7894)
                     /locus_tag="PLD98_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HQA05967.1"
                     /translation="MQALKEGQSKDDLPEEIRPKSGYGNTYAKLWWNRPSTTITRNFA
                     CPSSSRCIHPRDSRAMSIREGARLQSFPDTYKFYGSDSMKRLQIGNAVPPLVPYSVIS
                     TDASCKAVHISLMIKISFTLFSPSSFLYHMIFAQSSAFLARPLEKQKSVISFVF"
BASE COUNT         2091 a         1870 c         1853 g         2173 t
ORIGIN      
        1 ctcatggaga ttgtcggtca tggttgtgac catgctgtaa atcggcacat cgcatattgt
       61 ataggcggta tcccaaagca catacgcggc gccgaaccaa aagagcttca tgccctcatc
      121 caagccgctg ggtatagcga acataaagat tgtggtaagg gggattgcca aaagggaaat
      181 ttttagccac ggcaggctct tttgtccgct cttgaatttg accttgtcaa aaatccagcc
      241 gaaaatcggg tcgttaaatg cgtcccaaat cttaactata agcacgacgg cgctgatttt
      301 cgtcatactg acgcctatca tggcaaggta ggtcagaaga taggtggaca ctatggtgta
      361 aaaaatgttc tgacctacaa aatacgacca gtagcttacc ctttctctgc gggtagtcat
      421 gtacctttca ttgctgacat tctccatacc ctatttcctt tcagtcttta ttaaggcttt
      481 gcctgaatgt atttgagcgc atgaccgcgg tcaggacatg cattgcgcac ctttgcatct
      541 ctcccaaagt tatgccgtgg gttcttccgt aggttttaag cagggtaccg tccggttttt
      601 tgggaccgat tcgcttgccg ccgggcatta aaaggtccac tcccgacctt actctgtagg
      661 cgttgtccct tatttcgggg aactcggggc tctttgatga gcgcatccac cagtcggtca
      721 taacaagacc gtcaaagccc cattcttctc tgagtattct ctcgcaaagc tcatagtgat
      781 aatgacccca gacgccgttg attttgttgt aggaggtcat tatggcatgg ggctttgcct
      841 ctttgacgca gatttcaaag cctttaaggt agatttccct taacgccctt tcggagaccc
      901 ttgagtcgtt gtgcaggcgg ttatactcct gattgttgca ggcaaaatgc ttggggcagg
      961 ctgaaacccc cgcgctttga agtcccgcaa ccgcggcggc ggctattttg cccgacaaaa
     1021 tcgggtcttc tgaaaaatat tcaaaattcc tgccgcacag aacattcctc ttaatattca
     1081 tgccgggggc aaggagcaca tctgagtcct tttccttcat ctcgagcccg accttatggt
     1141 aaagctcctt aaccaagtcc ttgttaaagg tggaggcaag ggcagtgccg atgggaatga
     1201 gtgagcagct ttgctttaag cggattcccg acgggccgtc tgttgtggtg acgggggtta
     1261 cgcccttgtc acgaagggac tgaagcacac cgccgaaaac tcccgcgttg ccctcgggac
     1321 cgagggggct gttcattata taatctcccc ttgtgattgc ctcaagctcc tcaagggaga
     1381 gttgggcggt aaacctttca aggctgactt tgccctttgc cacatccttt aatttaaagc
     1441 ccatatcgcc cgtcattggc ttttcttcgg gcaggttgtc aagaataatc tgccttagat
     1501 ttaccgtgga ggcgggggca aggtccattt cgggcctgcg gtttccgttt tcatcctcca
     1561 cagccttgta aacgctgaag ggatacttcg gcgccgcagc ctctttaagt ttctccacca
     1621 cgatgttttc ttttattgtg aactcaaata ttttttccgc tgagcggaca tccgaaccga
     1681 gataaattct gtaaacgccc ttttcaatta catagcagga cttgttgccc gtagctcccg
     1741 atgagtcgta ggaggcaagc cgggtcgtgg gaatctcgag aaggacattc tcggtctgcc
     1801 ccgggaagat agcgcccgtc tttgcaaagt ccgccagctc ccttgagggg ttgcccagaa
     1861 ccccgcaggg cttttcaata tatgcgcaaa ggacatgctt tcccgcaaat atctcgccca
     1921 cattcttaac ggcggcggcg attttgattc cgtcggggaa ggatttgacc gcatgcttta
     1981 cgactttgaa ctctgtgtaa gaaagtccgt agccgaaggg gtataaaacc gcgtccttgt
     2041 taaaggtttc aaaccacctg tagcccacat aaatatcctc ttgatactcg ttatactcct
     2101 tgttgccgaa gttggagctg gagggatagt cgttataatc ccttgctatg gtttcggtga
     2161 gtctgcccga cggaacggcg tcgccgcaga gaaggtccgc tacagcgttt ccgctctcca
     2221 tgccgccctg ccagactatc ataacggcac cgagtttctc ccgatattct tctatccaag
     2281 aaaagtccat gatgctgccg atgttaagaa ggaccacaac atcgttaaaa tgctcggtta
     2341 ccttattaag catttccctt tcgtcgtcgg taaggtagta gctgcccttt tcgaggacat
     2401 tctccctatc ctcgcccgat gacctgccga tgacaaccac cgcatggtcg ctattctttg
     2461 cggcgtccgc cacgacggcg ctcaaaaggg gcatttcggg gtatgagcgg ggccagaacg
     2521 cccatgcgcc gtggtccacg gggttttcct ccacccattt ttcgtagata gagagaaggc
     2581 tttcgtttat tttgagcttc gggcagtttt tgattccctc ggcgaggttt atctgatagg
     2641 gtcttatgac atcgccgccg gagccgtagc ccgtgtagaa ccagtcaagc tgggttcttc
     2701 cgaaaacgga aacggtgctg ccccttttaa aaggcaaaac cctgttttca aggagaaccg
     2761 cgccctcagc cgcagcggcg cgaataagct cgggcgcgcc ttcggttatt ttgtactcgc
     2821 ccgtacccac ctgctgcatt tggcctacgc ccgtaagcct tgaatatgtt tttacaaaaa
     2881 actttataat atcttccttt atgctcattt caatcccttt cacgctcgtt tttggggaat
     2941 gtgaatactt ataatattat atattaggcg tataaaaatt taaatagact atttgtaaaa
     3001 actgtttaca atgtctaaat cggttttgaa atattggcgt gaaaaaaggc tgatgccaaa
     3061 aagcatcagc ccgaaatctt tgtcttttat atcgtgtttt tcttccagaa ggaggggaaa
     3121 aggagaacca gaatcgggta aacctcaagt cttcctatca tcatgcagat gctgagaacc
     3181 accttgctga gaacactgaa atcagcatag ttgcccacgg gaccgacaag tcccagaccg
     3241 gggccgatat cattgagcgt cgcgacgaca gccgatatgg tggtcggaag gtcaaagcct
     3301 tcaagggata tcaccaaaac cccggccgca aatatcgcca catagacaaa gaagaacatc
     3361 ttaattccag aaagaatctc ctcatccact gcgtgaccgt ttattctaac cgtctttacg
     3421 gctctggggt gattgagctt tgtgatttcc cttttgaaca tctttgaaag aaggagaatt
     3481 cttatacact taatgccgcc ggcggttgag cccgcgcagc cgcctataaa cataatcagc
     3541 agcaatacca tttggcttaa ggtgggccac tggttaaagt ccgcggtgac atagcccgtg
     3601 gtcgtgataa gagagcttac ttggaacgag gcgtagcgca gtgactctcc aattgtgctg
     3661 tagaccttgc cgtatatgtt agcaactatc agcacaatgg cgacggctat cactccgata
     3721 taatagagaa gctcctcatt ctttaaaaag ccctttacat tcttctttag gaggaaatag
     3781 tacaaggtaa aattaatgcc tgaaatcagc attgataaga taatgacggt ttcgatataa
     3841 acactgttat acgccgctat gctggcgttt ctgaccgaaa agccgcccgt gctgactgta
     3901 cccatggcgt gagctataga gtcaaacagc ggcatgccgc ccaaaagcag aagcacaatg
     3961 ataatgaagg taattgctat ataaattata taaagaattt cagctgtttg gcggattttt
     4021 ggcaccagct tgtctggcga agggcccgag gcttccgcct tcataatata caccgcattt
     4081 gcctttgtgg agggcaaaac ggctatcatc agaaccagaa ctcccatgcc gcccatccag
     4141 tgggtaaagc tgcgccagaa caaaattccc ttgggcaggg attcaacttc tcttaaaata
     4201 ctcgcgcccg ttgtggtaaa tcccgaagct gtttcaaaaa aggcgtctat caccgaaggg
     4261 atggagccgc tgattaaaaa cggcagagcg ccgaaaacgg taacggaaag ccagcccaga
     4321 gcggtaatcg caaaaccgtc gcgggcgtag atgttctggt tcggagcctt cagcctgtaa
     4381 agaataaagc ccgcaatcgc ggtaattacg attgaaatcg caaaggaata aaccgccttt
     4441 tccttataaa acaaggatac cagcattgaa ggcaccatgc acgcggcttc tattataagc
     4501 aatgagccaa tactctttaa tacttttcgg taattgataa gaaattcccc ccgttaaaca
     4561 atatcattaa ggtcggtcag ttctttgtct tttacaaaaa ggattacatt gtctttttct
     4621 tttatgacat cacttccgtg gggaattatt atatcgttct ttcttataat cgcggcaatc
     4681 agtatgccct ctttgagctt taagttttta agcggaacat cgagaaattt ggtagtcttg
     4741 ccggcggtaa actcgattgc ctccgccttg ttttccatta tcttgtaaag gacatcaacg
     4801 gggttgccca tggcgttttt gaggccgcgg acatagctta atataaagtt cgcggttatc
     4861 tgcttggggc tgactatatt gtcaatgccg agattcataa tagcgtcaaa gaagttctct
     4921 ctgctgattt tagctatgga cttcttaact ccgtactgct ttgccagcag agcggctatg
     4981 atgttgtcct catccctgtc ggtgatggcg acaaaggcgc ccatgctgtc aagattctcg
     5041 gaattgagta tggtttcatc ggagccgtcg ccgttgataa tcagagcctt gggcaaaagc
     5101 tctgacagct caaggcagcg gtctttgttc ttttctataa tcttgacctt tatctccatc
     5161 tcctgcaaat actttgcaag atagtaggcg attctgccgc cgcccattat cataacgctc
     5221 tttattttag gcatatagac gccgatgtgc ttgcagaact gaacaatcct tgagttgcgt
     5281 ccgacgataa agattttgtc tccgggcaag agcacaaaat ctcctttggg gattatcaca
     5341 tcatcgccgc ggagaacgcc gccgatgagt attcccttta tgcctttttc ggttatatct
     5401 ttaaggcttt tgttaacaat ggacatgttc tccgtaagtc gcatttccaa aagcactact
     5461 ctgccctttt taaatacctc cacattagcg gcagggggaa actcaataag ccttgcgatt
     5521 tcgcccgcga ccgcgtgttc ggggtttata atcatgtcca aatcaaggtc ggctttaagc
     5581 tgagtaagct cattcgcgta ttccggatcc ctgattcttg ctatggtatg ggccgctccc
     5641 agctttttgg ctgtaaggca gcataccata ttgatttcgt cgcttgttgt ggcggcgata
     5701 agaaggtcgg cctccttaat gcccgcttca agaagtgtgc ttgagctaag gccgttgcct
     5761 ttaatacaca taacatcaag atagtccgcg gcttttctca gcgcctgggg gtccttgtca
     5821 ataatagtga catcgttgtt ttcttttgag agactgtcgg caaggctgta gcctaactta
     5881 ccgtcgccta taattatgat tttcattttc tccaccttat tgtaacaggc aatgaattgg
     5941 actgtatgca tttacagttt ttgtaaattt ttgaaatcca ttcacattat agcaccgaga
     6001 gcttaaaaag caattataat aaatgccata tattactctt attttgcccc gtttttgtcg
     6061 aaaatgagaa tacgcccgcc ttaaacggac gggcattcaa gcaatcaagc aattaattga
     6121 gtatttaaga atatccccaa ttgttcataa acaatacatt tttaataact aaaatttgcg
     6181 atataattaa taatcagtta cgaaaaaagg agaaaagaac atgggagcat atactggagc
     6241 gcttgagttt ttcggcagtc ctcttgacat agtggcgaaa gaatttaact atgatgagga
     6301 caatattctg atccttcgcg gggatattgc ggcactgcac tcaaagtatc ccgaaacata
     6361 cagaaatctt gaaagctgca cgcaccacag ggataacaga accgccctgc agtacgctca
     6421 ggatttggta gcttcttggc ttattgaaga ttacttttag agcttctgca atccgattat
     6481 tataccctca gaatgcacgg agtcgacaga acaaggagaa ttcttgcgag ttctcagacc
     6541 tccaccgtca gtgattttct gattactcgc gacggcgttg aaataaaact tgagcttatg
     6601 aacgattata cgggctattg gcgcagatac aatgtgctcc atctgaggga ttttaaattc
     6661 aatcaattaa aaaaatctag tgccgttctt atctgcatat ccgttcccac gggtgaattc
     6721 gccttatatg acttcaacga ggatattagg gcaagatata tgccctctca ccgcccatat
     6781 gggggaaagc ccgcgtatga aatcggcatt cagaatgaaa tgatagagaa aatcggtgaa
     6841 aacaatatcc agatggcgat aatcgaaata tttgacagga ggagagcgta atgagcacca
     6901 gagacagagc aggcggctgg aagcacgcaa agctgacggg gcataagaat gaagccgcaa
     6961 tagaagagct tataagaaac gatattgagt atcaaaaagc ttttctcaga aagataggga
     7021 aacccgaaga agaaatagcg tacatatccg ttggcgggct gcatgaaaca aatgttgaca
     7081 gcgtgtttcc aaatgaaaaa acaaagtcca aaactgacat gcagatagtt ttgaaagacg
     7141 gcaacaggta taatatatcc ataaaaaaga gcctcggcgg gcaggtgtat ctcatatccg
     7201 attcccgatt tattaaaggc tttgagttac agtacggaaa ggaaatcccg caaaaggtca
     7261 aaagggcgat agagcttttc tggggttccg ctcccgatgt aaaggagata atagaaaaac
     7321 acggtcaaaa aagggattac gaaacaagaa aacatcgcct tgtcgccgaa actcttttga
     7381 gatacaatga agaactttat tttgaacttc ttaattggtt cgctcaaaac acaaacgaaa
     7441 taacggattt ttgcttttcc aaaggtcttg ccaaaaacgc agaagactgg gcaaatatca
     7501 tatggtataa aaacgagctt ggtgaaaaca gcgtaaacga gatttttatc ataagtgaaa
     7561 tgtgcaccgc cttgcaggaa gcgtcagtgg aaatgactga gtacggcaca aggggcggta
     7621 cggcgttgcc gatttgtagc cttttcatgc tgtcgctacc atagaacttg tatgtatcgg
     7681 gaaagctctg aagccttgcg ccttccctta tggacattgc ccttgaatct ctggggtgaa
     7741 tgcatcttga agatgagggg caggcaaaat tccttgttat tgtggtggac ggtctgttcc
     7801 accagagctt tgcgtatgta tttccgtagc cgcttttggg acggatttcc tcaggcaaat
     7861 cgtccttgga ttgcccctcc ttcaaagcct gcatgattct gattaaatgc tcgccgtttc
     7921 tcggtgcagt gtgctcggtc aattgctcgc tgccctcgcg gacaaatctt aggaaatcgt
     7981 tgtcagg
//
LOCUS       DAONVQ010000094         7848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_7037_length_7848_cov_4.480046, whole genome shotgun sequence.
ACCESSION   DAONVQ010000094 DAONVQ010000000
VERSION     DAONVQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7037_length_7848_cov_4.480046"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="PLD98_06690"
     CDS             complement(<1..1469)
                     /locus_tag="PLD98_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HQA05968.1"
                     /translation="MSGVLTSFYDYLASLGQYCGRYTYLLFKGLFTIIKKPIVFMFAA
                     FASLYRGTAGLVFRTFKAVLSETKLFVKEVKDTSERIKNDIIANPKKAPSIIWGYILK
                     AIIRHRVVFKYFLNLILPILGLLLLIYTINHWNRSIFALEININGETIGYVSAESVFV
                     EARDIAKDRLGISSGFSAMSTTQLLDAPVYSLSLIKINQLTDADTIADKLIERSASNI
                     TNACGIYINDEFLCAVKNETDAKRVFDSLLAPYEESGEYDVVSFVEKIDYVQGLYPDT
                     EDIMWDAARLKEKLQSKKQESLYYKIEEGDLVSTIASKFGVTSSQIRRLNPNLDEKRL
                     RIGQEILVSEEVPFIRVKTAKIKVTTEVLPYKTVEVLTDAYYQGTKKTIREGVNGTQQ
                     VTSLVTYINGTEYSTEEISRVTIKEPVDKRVQVGTKKYSGSSPSSGGGKPTKYTGAKL
                     TWPVIGAYSISSGYGYRNRAMHRGVDIVASGGSSKGKVIV"
     gene            complement(1647..3470)
                     /locus_tag="PLD98_06695"
     CDS             complement(1647..3470)
                     /locus_tag="PLD98_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQA05969.1"
                     /translation="MASIDVDFTGGGKKNVVKETVIPPERAGLKTFINILVTILTAVI
                     AFYFMLPPVNFKAPEFYLYLLIVFGSYIASSAITSKAIVRPEYMPYVKRNSLVPIILI
                     VVVGVIFAGGYISSATLLRAKAYSRILKIEKTDFSKSVTVIDSLSGFKNVPMIDAAAA
                     GKLADKTLGDLYLESLESQFEVDYDYSTQINYKQSPVRVFPLRYGDIFKWLSNRAEGL
                     PGYIIVNMNTQKADMVMTEGGIKYSPSEHFGRLLKRHLRFSYPTYIFGQPALEINEDG
                     RPYWICERMDKKVGLLGGDDVVGAVFVDAINGEHKYYDIEEIKSGVSKDGENLSWVDQ
                     IYPADLLMKQYNFFGKFSNGFFNSIIGQSGVKLTTDGYNYLALHDDVYMYTGVTSASS
                     DQSIIGFALINQRTKEAFFYQTSGATEKAAMESAEGIVSDKGWKATFPLLLNLDGEAT
                     YFMSLKDNNNVIKSYAMVNVSQYSDAVRSPSDDNPDLKACITAYVSKLAARGVHLNID
                     LTGTAPGGSSGGDTPTQETKEVSGSIAEIRPAVISGNTVYFIRLQNSKTYYTASAADF
                     GSLPVILNVGDYVTITVPMSDAGIVQAKDIKLSVPQTEQAG"
     gene            complement(3618..5063)
                     /gene="thyX"
                     /locus_tag="PLD98_06700"
     CDS             complement(3618..5063)
                     /gene="thyX"
                     /locus_tag="PLD98_06700"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HQA05970.1"
                     /translation="MDFKDRIYSVAEIVGISSMGEKIPAAAGRISTLEGDTHEILEKS
                     QDAEKNANLIKKVTRSNHTSILEHTVYNIAFRNVSILAEQFMIEFRLASFTVKSRRYV
                     NFGSMGFYVPELDGDICQDEFKKSVRACLEDYNALLDRGIPKEDARFILPYCVFSNFY
                     CTVNARELLNIIRSALYGRGRLYPEIKSLGEQLMAQIAQMTPGIAASLSDSRPELSDR
                     LSLSYLFKAKEGANERPKNANEKSVELISYTSDAEKTVAKAALTASTQYPLSEIEKLL
                     EDDGNLTTVLDKVIACSRPRSLEAAVYTFRINGVSLSEITHFARHRMQSIEIPSYTLI
                     DGKRYVIPETVKKDPVALEIYINAFERMDEVHKKLKNAGMPEEELVYLRLSGSKLDIM
                     VTMNARELLLFFRLRTCTRAQWEIRGHAIEMLRILREVSPGIFNRFGPSCFVSKCTEG
                     TLTCGRMDDMKKIFDPMNKSLSEDVLKEQTA"
     gene            complement(5178..6167)
                     /locus_tag="PLD98_06705"
     CDS             complement(5178..6167)
                     /locus_tag="PLD98_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQA05971.1"
                     /translation="MGKKLSKGAKIALAGAAGAGLFYFAAGAGFYEAFLTSKAVHKKN
                     KPHLPPEAEKERCRALEKYENASEWYAAQDDNDLELISLRGETIRARYIPAEVNRHKY
                     LICIHGYTSSPYRMAGPAYEFYKRGFNVLMPYLGGHGLSDRKTVSMGYHDRLDIVDWI
                     NYLVTFDEDAEIVLYGVSMGAATTLMTTGEDLPDNVKCAISDCGFTTAWEEFMVQCKN
                     VVHLPPFPFLYALRRYAIIRDGFDPRKCSPLDCVQRSKTPTLFIHGDKDEFVPFWMLD
                     VLYDNAVCEKERLVVEGAVHANSDEVNPELYWKTVFSFVDKYLTPAMAEESVA"
     gene            complement(6309..6773)
                     /locus_tag="PLD98_06710"
     CDS             complement(6309..6773)
                     /locus_tag="PLD98_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQA05972.1"
                     /translation="MISIEVIDRKHCEALSLIVSTDTELARHLYGQGKAPAPTSEYEF
                     FEQCKRWEHKNGGFCYAVLLDGVAIGTASYVPIRGGETAGVGFYISSKYWGKGYGTEL
                     LNELKNTLKEKGFKYITGNMPKSNIASKRVFEKCGAKFKERGDRVYSVIELQ"
     gene            6936..>7848
                     /locus_tag="PLD98_06715"
     CDS             6936..>7848
                     /locus_tag="PLD98_06715"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQA05973.1"
                     /translation="MRIGIDLGTDSIIAYAEGKGIVLSEPSVVVCDAFTGKPVAMGGA
                     ALKMTGRVPGSLNVVRPLKDGIVSDFRLTELMLRYYIQKICKSAVLRPFVAVCVPSTV
                     TRFEKKTILNVIMAAGAGNACLIEEPLAAALGAGIGTKKPRGSVVVDIGGGTTDTAVI
                     TMGSIAVSSSVKAAGGSITNAIIRYLSRERDIEVGYLTAEEIKCTIGSAVLREEETAM
                     VANGKSIVSGMPLSFEINSTEVYYAIKDELDHILQGTLQVLERTPPELMSDIMDTGLT
                     LTGGGSLLYGMAEMFKRHTGIDTDIAGE"
BASE COUNT         1848 a         1827 c         1974 g         2199 t
ORIGIN      
        1 acgatgacct tgccctttga ggaaccgccg gaggcgacga tgtcaacgcc tctgtgcatt
       61 gcgcggtttc tgtagccgta gccggagctt atggagtagg cgccgattac gggccatgta
      121 agttttgctc ccgtatactt tgtgggcttg ccgccgcccg atgacggcga tgagccgctg
      181 tacttcttgg tgcctacctg aaccctcttg tcaacgggct cttttatggt gactctcgag
      241 atttcctcgg tggagtactc ggttccgttt atataggtta caaggcttgt gacctgctga
      301 gtgccgttaa cgccctctct gatggtcttt ttggtgccct gatagtaggc gtctgtaaga
      361 acctcaacgg tcttataggg cagaacctcg gttgtgacct tgattttcgc ggtctttacc
      421 ctgatgaacg ggacttcctc ggacacaagg atttcctgac ctattctcag gcgcttttcg
      481 tcaaggttgg gattgagcct tcttatctga gaggaggtta cgccgaactt tgaggcgatt
      541 gtggaaacta aatctccctc ctcgattttg tagtaaaggc tctcctgctt tttggactgg
      601 agcttctctt taagccttgc ggcgtcccac attatatctt ctgtatcggg atagagtccc
      661 tgcacatagt cgattttctc cacaaagctg accacatcgt actcgcccga ttcctcatag
      721 ggggcaagca ggctgtcaaa tacccgcttt gcgtcggttt cgtttttgac cgcgcacagg
      781 aattcatcgt taatatagat tccgcaggcg tttgtgatat tgcttgcgga gcgctcaata
      841 agcttgtccg ctattgtgtc tgcgtcggtc agctgattaa tctttattaa ggaaagcgaa
      901 taaacgggcg cgtcaaggag ctgcgtggtg gacatggcgg agaaaccgct gctgatgccc
      961 agcctgtcct tcgctatgtc ccttgcctcg acaaagacgc tctccgccga tacatagcct
     1021 atggtttcgc catttatgtt tatctcaagg gcaaaaattg acctgttcca gtggtttatg
     1081 gtgtaaatga gaagaaggag accgagaatg gggagaataa ggttcaggaa atatttgaat
     1141 acgaccctgt gccttattat cgccttcagt atgtagcccc agatgattga tggtgctttt
     1201 ttgggatttg ctataatatc attctttatt ctctcggaag tatcctttac ctcttttaca
     1261 aagagcttag tttccgacag taccgctttg aaggtacgaa aaacaaggcc tgcagtgccc
     1321 ctgtaaagag aggcaaaggc tgcaaacata aatactatgg gcttttttat aatagtaaat
     1381 aagcctttaa agagcagata tgtatatctt ccgcaatact gcccaagaga ggctaaatag
     1441 tcgtaaaaac ttgttaatac gccgctcaag agtctattct ccttgtaaaa atgaaaaggt
     1501 tacaaaagag ttacaaacag attatactat ataacttcct ctttagcaag tccatctttt
     1561 tgccagcaaa tcgggcgatt aactgagaat aacaaaaatc agggcttatt tgccctgatt
     1621 ttctttcata ttcatttata ttagagttac cccgcctgtt cggtctgagg cactgagagc
     1681 ttaatatcct tagcctgaac aatacccgca tcggacatgg gaaccgtaat tgttacatag
     1741 tcgcccacat tgaggattac ggggagagag ccgaaatccg cggctgaggc tgtgtagtag
     1801 gtctttgaat tctgcagcct gataaagtat acggtgttgc cgcttatgac agcgggtctg
     1861 atttcagcta tgctgcccga aacttccttg gtttcctgag tgggggtgtc gccgccggat
     1921 gagccgccgg gcgctgtgcc ggtaaggtca atattaagat gcactcccct ggctgccagc
     1981 ttgcttacat atgcggtaat gcaggcttta aggtccgggt tgtcgtcact ggggcttctt
     2041 acggcgtcgg aatactggct gacattaacc atggcgtagc tcttgataac attgttgttg
     2101 tctttaaggg acatgaagta ggttgcctcg ccgtcaaggt tcagaagaag cgggaatgtt
     2161 gccttccagc ccttgtcgga aacgatgcct tcagcggact ccatagctgc cttttcggta
     2221 gcgcctgagg tctggtagaa gaatgcctct ttggttctct ggttgataag ggcaaagccg
     2281 attatcgact ggtcggagga agcggaggtt acgcccgtat acatatagac atcgtcatgg
     2341 agcgccagat agttgtagcc gtcggttgtg agcttaacgc cgctctgacc gatgatggag
     2401 ttaaagaagc cgtttgagaa cttgccgaag aagttgtact gcttcataag caggtcggcg
     2461 gggtaaatct ggtcaaccca tgaaaggttt tcgccgtcct ttgagacgcc gctcttgatt
     2521 tcctcaatgt cgtaatactt gtgctcgccg tttatggcgt caacaaatac cgctccgact
     2581 acatcgtcgc cgccgaggag accgaccttt ttgtccattc tctcgcaaat ccagtagggt
     2641 ctgccgtcct cgttaatctc aagggcgggc tgaccgaaaa tgtaggtggg ataagagaat
     2701 ctgagatgac gctttaagag tcttccgaaa tgctccgaag gggaatactt gattccgccc
     2761 tcagtcataa ccatatcggc cttttgggta ttcatgttca caatgatata gccgggaaga
     2821 ccctctgccc tgttggacag ccatttgaat atgtcgccgt atcttaaggg gaataccctg
     2881 acggggctct gcttgtagtt aatctgggtg gaatagtcgt agtcaacctc aaactgggac
     2941 tcaagggact caagataaag gtcgcccagt gtcttgtcag cgagtttgcc cgccgcggcg
     3001 gcgtcaatca tggggacatt cttaaagccg ctgaggctgt ctataactgt aacgctcttt
     3061 gaaaagtcgg ttttttctat cttgagtatc ctgctgtacg cctttgccct taaaagggtg
     3121 gcggaggaga tatatccgcc tgcgaatatg acgcccacaa caacaatcag gataatgggc
     3181 acaagcgaat tgcgcttaac atagggcata tattcgggtc ttacaatcgc ctttgaagta
     3241 atagcggagc ttgcgatata agagccaaag acaataagca gatagaggta aaactcggga
     3301 gccttgaagt ttacgggcgg gagcatgaag tagaaagcga taaccgccgt aagaattgtc
     3361 acaaggatat ttataaatgt cttaagtcct gctctttcgg gcggaatgac ggtttcttta
     3421 accacattct tcttgccgcc gccagtgaaa tcaacatcta tgcttgccat caggtcaact
     3481 ccttttaaat tagcaacacc tatgtgccgt ttgtgttaaa ttacagtata cagtaaaagc
     3541 aaacttatta caagaaacta ataatgaata ttctcttaac taattattgg tgccttttta
     3601 aagccgtaaa ccaaacttta ggcggtttgc tcctttaata catcttcgct gaggctttta
     3661 ttcatggggt cgaagatttt tttcatatcg tccatcctgc cgcaggtgag cgtcccctcg
     3721 gtgcattttg aaacaaagca gctgggaccg aaacggttga agatgccggg ggagacttct
     3781 ctgagtatcc tcagcatctc aatggcgtga ccgcggattt cccactgtgc tcttgtgcag
     3841 gttctgagtc tgaagaacag cagcagctcc cttgcgttca tggtgaccat tatgtccagc
     3901 ttgcttcccg aaagtctgag gtaaacaagc tcctcctcgg gcatacccgc gttctttagc
     3961 tttttatgca cctcgtccat tctttcaaag gcgtttatat aaatctcaag ggctacgggg
     4021 tccttcttta cggtttcggg gattacatag cgtttgccgt caattaaggt gtaggaggga
     4081 atctcgattg actgcattct gtgacgggca aagtgggtaa tctcagagag ggaaacgccg
     4141 tttattctga aggtgtagac cgcggcctca agggagcggg ggcgggagca tgcgataacc
     4201 ttgtccagca ctgtggtaag gttgccatcg tcctcaagga gcttttctat ttcgcttaaa
     4261 ggatattggg tagatgctgt gagagctgcc ttagcgacgg ttttttccgc gtcgctcgtg
     4321 tatgagataa gctccacgct tttttcgttg gcgtttttag gtctttcgtt tgcgccctct
     4381 tttgctttaa acaggtagct gaggctgagc ctgtcgctga gttcgggtct agagtcggaa
     4441 agggacgctg cgataccggg ggtcatttgg gcgatttgtg ccatcagctg ctcgccgagg
     4501 ctcttaattt ctggatagag ccttcctctg ccgtaaaggg cgcttctgat gatattcaaa
     4561 agctctcttg cgtttactgt gcagtagaag ttgctgaaaa cgcagtaggg caaaatgaac
     4621 cttgcgtcct ctttggggat gcccctgtct aagagagcgt tgtaatcctc aaggcaggct
     4681 ctgacactct ttttgaactc atcctgacag atgtcgccgt ctagctcggg aacataaaag
     4741 cccatggagc cgaaatttac ataccttctt gactttaccg taaaggaggc aaggcggaac
     4801 tctatcataa actgctccgc caggatggac acatttctga aggcaatgtt atacactgtg
     4861 tgctcaagta ttgaggtgtg gttagaacgg gtaaccttct ttattagatt tgcgttcttt
     4921 tccgcgtcct gggatttttc gagtatttcg tgggtatccc cctcaagagt ggagattctg
     4981 ccggcggcgg cggggatttt ctcacccatg ctgctgatgc ccactatttc cgctacggag
     5041 tatatcctgt ctttaaagtc cattaaaaca ccccttaacc tgagctttta ttactgcatt
     5101 tgagtttatc ataaattttt ctttgtgtaa atcactaaag caaaaaggga gccggtaggc
     5161 tcccttgatt atttaattta tgccacgctt tcctctgcca tggcgggggt aagatactta
     5221 tccacaaacg agaatacggt tttccagtac agctcggggt ttacctcatc ggagtttgcg
     5281 tgtaccgctc cctctactac cagcctttcc ttttcgcaga cggcgttgtc gtagaggaca
     5341 tccagcatcc agaagggcac gaactcatcc ttgtcgccgt ggataaagag ggtgggtgtt
     5401 tttgacctct gaacgcagtc aaggggcgag cattttctcg ggtcgaagcc gtccctgata
     5461 attgcgtatc tgcgaagggc gtagaggaag gggaagggcg ggagatgcac gacattcttg
     5521 cattgcacca taaactcctc ccacgctgtg gtaaatccgc agtcggaaat ggcgcacttt
     5581 acattgtcgg gcaaatcctc tcccgtggtc ataagggttg tggcggcgcc catggatacg
     5641 ccgtacaata ctatctcagc atcctcatca aaggtgacga gatagtttat ccagtcgaca
     5701 atgtcaagtc tgtcgtgata gcccattgaa acggttttcc tgtcactcag tccatgtccg
     5761 ccaagatagg gcatcagaac attaaatccc ctcttgtaaa actcatacgc gggacctgcc
     5821 atgcggtagg gcgagcttgt gtagccgtga atgcagatta aatatttgtg ccggtttact
     5881 tcagcgggta tgtaccttgc gcggattgtt tcgcccctta aagatataag ctccaaatcg
     5941 ttgtcgtcct gggcggcgta ccactcggaa gcgttttcat acttttcgag ggctctgcac
     6001 ctttcttttt ccgcctcggg gggcaaatgg ggtttgttct ttttatgtac cgctttgctt
     6061 gtcaggaatg cttcgtagaa tccggctccc gcggcaaaat agaaaagacc tgctcccgca
     6121 gcgccggcaa gggcgatttt tgcgccctta ctcagtttct tacccataat acaactcctt
     6181 gcgtcagctt aagaatttat tgcacaaaac gcctttcggc gcgacctttc aaagcaaaga
     6241 atatcgatta aaaaatatcg atacaattat acccttgttt ttaaattaat gcaatgttga
     6301 aagggaaatt actgaagttc tattacagaa tatactctgt cgccgcgttc cttaaatttg
     6361 gctccgcact tctcgaaaac tctttttgag gcaatattgc tcttgggcat gttacccgtg
     6421 atgtatttaa agcccttttc tttaagagtg tttttaagct cgtttaaaag ctcggtgccg
     6481 tatcccttgc cccagtattt gctggaaata taaaagccca ctccggctgt ttcgccgccc
     6541 cttatcggga catatgacgc ggttccgatt gcgacgccgt caagcaaaac ggcatagcag
     6601 aatccgccgt ttttgtgctc ccatcttttg cactgctcaa agaactcata ttcggatgtg
     6661 ggggcgggag ctttgccctg cccgtaaaga tgtctcgcaa gctcggtatc cgtggaaact
     6721 attaagctta aagcttcgca gtgctttctg tcaattactt ctatactaat caattcatcg
     6781 cctcctacat ttagtttact ttaaatacaa aattaaagca agtcgccaat tctttctgcg
     6841 gttatataag gaaatcggcg tattgaagta aagttcttat tgtggtatac ttttttagaa
     6901 ttttatgttt ctttggggga atagttttgc tcggaatgcg tataggcatt gacctgggca
     6961 cagactcaat aatagcttat gctgagggca agggcattgt actgtcagag ccctctgtcg
     7021 ttgtttgcga cgcgttcacg ggcaagcccg tcgccatggg cggcgcggct ctcaaaatga
     7081 cgggcagggt ccccggttct cttaatgtgg taagaccttt aaaggacgga atcgtctcag
     7141 acttccggct gactgagctg atgctcaggt actatataca aaaaatctgc aagagcgcgg
     7201 ttttgagacc ctttgttgcc gtttgcgttc ccagcacggt cacaaggttt gagaaaaaaa
     7261 ccatcctcaa tgtaattatg gcggcgggcg ccggcaatgc ctgccttatt gaagagcccc
     7321 ttgccgcggc attgggcgcg ggaatcggca caaaaaagcc gagaggcagc gttgttgtgg
     7381 acatcggcgg cggaaccacc gacacggcgg taattaccat gggcagtatt gcggtttcaa
     7441 gctccgttaa ggcggcggga ggcagcatta ccaacgctat aataaggtac ctaagccgtg
     7501 aaagggatat tgaggtgggc taccttaccg ctgaggagat taaatgcact atcggcagcg
     7561 cggttctcag agaggaagaa accgcaatgg tcgcgaacgg caagagcatt gtgtcgggaa
     7621 tgccgctcag ctttgaaata aactctaccg aggtttatta cgcaataaag gatgagcttg
     7681 accatattct tcagggcacc cttcaggtgc tggagcgcac tccccccgag cttatgagcg
     7741 acattatgga tacgggtctg actttaacgg gcgggggttc cctgctttac ggcatggcgg
     7801 agatgttcaa gcgtcatacg ggaatagata cggatatcgc tggcgagc
//
LOCUS       DAONVQ010000095         7723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_7176_length_7723_cov_5.012911, whole genome shotgun sequence.
ACCESSION   DAONVQ010000095 DAONVQ010000000
VERSION     DAONVQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7176_length_7723_cov_5.012911"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PLD98_06720"
     CDS             <1..1088
                     /locus_tag="PLD98_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQA05974.1"
                     /translation="AGNPAPEVFPADVIENIASKILRQQPVTALQYGVTEGYLPLRER
                     ISEDLKARFNIGREFDEIIITAGAQQVVDLAAKVFLNEGDTVICESPSFIGSLNCFRS
                     YNACLKGIPVESDGMDIELLEKALETEKNVKMIYTIPNFQNPSGYTMSLEKRKALYEL
                     ALKHNVIILEDNPYGELRYTGEDLPSIKSFDDEGIVIYAGSFSKVLSPGLRVGYSVSP
                     AEITAKMTVGKQASDVHSPMICQMIVDRWLAECDTAEHIGFIRSVYSRKLNLMCDCLD
                     SEISGFISYVRPLGGLFIWCELDRNINMLDFCKAAAAENVAVVPGTAFMASESDRTNC
                     FRLNFSTPTDEQIVEGVKILKKVCEKF"
     gene            1112..2131
                     /gene="trpS"
                     /locus_tag="PLD98_06725"
     CDS             1112..2131
                     /gene="trpS"
                     /locus_tag="PLD98_06725"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQA05975.1"
                     /translation="MDGNIQVTEKKQVVLSLVQPTGTPTLGNYLGAFKNWAKMAEDYD
                     CLFGAADLHSITVRTEPKVLKSRIIEIYAILLSLGLNPEKNIIFVQSQVPQHSQLGWI
                     LNCYTQFGEAARMTQFKDKSKKYAENVNVGLFSYPTLMAADILLYQADFVPVGEDQKQ
                     HLELARDIATRMNNLYGNLFTVPAPFIGKAGAKVMSLQEPTKKMSKTDPNTKSFISML
                     DTPDVILKKIKSAVTDSEGKVYYSEEKPGISNLMSIYSACTGLSYEEIEKEFDGKGYG
                     DFKAAVAEAVIEELRPFRAEFDRIINDKAYILSSMEKGAERAEKRARRTLDKVMKKIG
                     FVSYR"
     gene            2156..4378
                     /locus_tag="PLD98_06730"
     CDS             2156..4378
                     /locus_tag="PLD98_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQA05976.1"
                     /translation="MKRSVKLNSNWFFSMVVPQDLTAIPENAESVTVPHTWNIYDLKR
                     RGSNTRGRYCYQRELPVPLELSGYRLFLEFKGAGPLAELYIDGKKVGTHEGGFTSFCF
                     EITDFVIPGRNHVITMLTDSTERGKCCPVNPHFAVFGGIYRDVSLIVSGKSHFALTED
                     GSKGVFISTKMAGSDALVAVAANISNPINYDVVSFTIYDSAENAIGTATAHPKQAEAI
                     MRIHNPSLWNGTASPYLYTLRAQLIRDGVILDRVDIPFGIRNIEIKSSGFYLNGKRTE
                     IKGISLYQDRAKTGIAASDEQIRKEIQLLKELGANAVRLNCFPPDESVLDYCDSEGIA
                     VWLEIPFGNNIGKNSPAFKNARQQLTELIKQYYNHPSIVAWGINCPKWHAGDSSVAFA
                     QAEDLARLARSLDKTRVCVFTEQVDADTPVFPQSAGAFNADIDLLDEFSAERALRTLE
                     LMSEANPDLPISVSFSTELSPEKSANVLLENAFKAADERGFIWGVFAGPLFDFAKRTR
                     EIGTEFEITEDIPAEDEPTEETSEETKAEETAEIAAPAEETAEETEAEETQETESEET
                     ADNAESEESEKAEEAEAEPAEESPEEAAASEKAEEEKAEATERTASAPAINGYAENIE
                     ISSCGFVSYDRQQKRDSFYLIKAMWSKSKFIYIAEENGSFRSKRKCSIRVYSNLKDLR
                     LTVNGKEKKSKTLHSENGVFIFNDVVLAKGENSIVASCDYEIGITDKVTIIRQRQAGK
                     "
     gene            4400..5125
                     /locus_tag="PLD98_06735"
     CDS             4400..5125
                     /locus_tag="PLD98_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HQA05977.1"
                     /translation="MKIVVSENSKALGAAAAQHIAETINNAIAEKGNARIVLSTGASQ
                     LDMFEALLQLNVDWSKVEMFHLDEYVGIEETHKASFRKYLKERFASKINLKNAYFLDG
                     SEEVTKFVTEKLLEAEIDLGVIGIGENAHIAFNDPPADFDTKEAYHIVKLDDACKRQQ
                     VREGWFPTVEDVPTHAVSMTCYQILQCKKIISAVPHEVKAEAVYKTLTEKVNNNTPAT
                     LLKTHPNWTLYLDKNSASKVFPV"
     gene            5212..5676
                     /gene="spoVAC"
                     /locus_tag="PLD98_06740"
     CDS             5212..5676
                     /gene="spoVAC"
                     /locus_tag="PLD98_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HQA05978.1"
                     /translation="MAENNQHQDKAYMAMAEKASPNSHILKNAVKAFLIGGLICAFGQ
                     FLFFIYEKWGIDEETVRSLVPTTIIAITAVLTGLGWFDDIAKHAGAGTLVPITGFANA
                     VVSPAVEYQTEGRILGTGAKMFTIAGPVIVYGCGTATLYGVIYYICSMFLGA"
     gene            5691..6713
                     /gene="spoVAD"
                     /locus_tag="PLD98_06745"
     CDS             5691..6713
                     /gene="spoVAD"
                     /locus_tag="PLD98_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HQA05979.1"
                     /translation="MAQRQGRFVLKLPNKPKILATAAVVGPKEAEGPHGPDFDNCHSD
                     DYVGQETWEKAESVIHKEAIERAINKAGLSPLDIDMVFAGDLMDQEIASTLGIRELNI
                     PFVGLFGACSTMALGLALAGLMADTHAANYAVASTSSHFCSAEKQFRYPLAYGAQRSP
                     TAQRTVTGAGAAVVAANGPKRAPYIDAVFLGRIRDLGIKDAANMGAAMAPAAATTIID
                     FMHDTGTTADDYDLILTGDLGKVGSTLVRELMLTRENMDISKVHNDCGMMIFDLEKQD
                     VHAGGSGCGCSAAILCSYIYKELEKGNLKSVLFVGTGALMSPTLTQQGETIPSIAHAV
                     LIRSGC"
     gene            6733..6933
                     /locus_tag="PLD98_06750"
     CDS             6733..6933
                     /locus_tag="PLD98_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05980.1"
                     /translation="MANNKIAKVNGNKALTYISKTLDNIKVIDTASKTIKFLSYLKIA
                     VGAAAAAIVVYQLYSVIKSEMK"
     gene            complement(6985..>7723)
                     /locus_tag="PLD98_06755"
     CDS             complement(6985..>7723)
                     /locus_tag="PLD98_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA05981.1"
                     /translation="QDYFIDVLNFATLAAFTGLPGSIVHPISYSLVPWFRRKFSSRTL
                     YILGAYIGDILMVPVFFVGAIGGKKNGLYKKIIPMAIALTVWETVFMLFYGVRKVIPS
                     ELYNEAMDYCEWKNGYRTEAMTSVAKGLATKLASLFSNMVQLQIKKLIKYDQTLYISG
                     KQQSDDTKFGLFAMFTIVPFATTSLGIIPMLFYDLAGEKRERMYAELLARRAEMSQSA
                     TSGSAEDLERLAEMQMKVGEKNKNKEL"
BASE COUNT         2088 a         1805 c         2017 g         1813 t
ORIGIN      
        1 ccgcgggcaa tcctgctccc gaggtctttc ccgccgatgt tatagagaat atcgcctcaa
       61 aaatactgcg tcagcagccc gtcaccgctt tgcagtacgg cgtgacggag ggatatttgc
      121 ctttaagaga gagaatatcc gaagatttaa aggcaagatt taacataggc agggaatttg
      181 atgaaataat aataacggcg ggcgctcagc aggttgtgga ccttgccgcg aaggtgtttt
      241 taaacgaggg tgatacggtc atttgcgaat ccccgtcgtt tatcggctct ttaaactgct
      301 tccgctcata caacgcttgt ttaaagggca tccccgtcga aagcgacgga atggatattg
      361 agcttcttga gaaggctctg gaaaccgaga aaaatgtaaa gatgatttat accataccca
      421 atttccagaa cccttcgggc tatacaatgt cccttgaaaa gagaaaggct ctttatgagc
      481 tggcgctgaa acacaatgtc attatcttag aggacaaccc ctacggagag ctgagataca
      541 ccggcgagga cctgccttca atcaagagct ttgacgatga gggcattgtg atttacgctg
      601 gctccttctc aaaggttttg tcgccgggtc tgagagtcgg ctactctgtg tctcccgctg
      661 aaattacggc gaaaatgacc gtcggcaagc aggcgtcgga tgttcacagc ccgatgattt
      721 gtcagatgat tgttgacaga tggctcgctg agtgcgacac cgcggaacat atcggcttta
      781 tccgctcggt gtactcaagg aagctaaatc ttatgtgcga ctgccttgac agtgaaatca
      841 gcggatttat tagctatgtg cgcccccttg gcggactttt catatggtgc gaacttgaca
      901 gaaatataaa tatgctggat ttctgcaagg cggctgccgc ggaaaatgta gcggttgtac
      961 cgggtacagc tttcatggca agcgaaagcg accgcaccaa ctgctttaga ctaaacttct
     1021 ccacccccac cgatgagcaa atcgtcgagg gtgtaaagat actcaaaaaa gtttgcgaaa
     1081 aattctaaac tcagcttgaa aggacaggaa aatggacggc aatattcagg taacggaaaa
     1141 aaagcaggtt gttttaagtc ttgttcagcc caccggcaca cccacattgg gcaattatct
     1201 cggagccttt aaaaactggg ctaagatggc tgaggattac gattgccttt tcggcgcggc
     1261 tgatttacat tccatcaccg ttcgtaccga gcccaaggta ttaaaaagca gaataatcga
     1321 aatatacgct attctcttat ccttaggtct taatccagaa aagaacatta tttttgtgca
     1381 aagccaggtg ccgcagcaca gtcagcttgg ctggattctg aactgctata cccagttcgg
     1441 cgaggctgca agaatgactc agtttaagga caagtccaaa aagtacgctg agaatgtgaa
     1501 tgtgggtctg ttctcctacc ccacccttat ggcggcggat atacttcttt atcaggcgga
     1561 ctttgtcccc gttggcgagg accagaagca gcacttggag cttgcaaggg atattgccac
     1621 aagaatgaac aacctttacg gcaatttgtt caccgtcccc gctcccttta taggcaaggc
     1681 gggcgcaaag gtaatgtcgc tccaggagcc cactaagaag atgagcaaga ccgacccgaa
     1741 tacaaagagc tttatttcga tgctcgatac tcccgatgtg attttaaaga agattaagag
     1801 cgcggtcacc gactctgagg gcaaggttta ctacagcgag gaaaaacccg gaatcagcaa
     1861 ccttatgagc atatactcgg catgcacggg tctttcctat gaggagattg aaaaagagtt
     1921 cgacggcaag ggctacggcg actttaaggc tgctgtcgct gaggctgtta tcgaagaact
     1981 tagacccttc agagcggagt ttgacagaat tataaacgac aaggcatata ttctgtcatc
     2041 catggaaaag ggcgccgaaa gagcggaaaa gcgcgcaagg cgcacccttg acaaggttat
     2101 gaaaaagatt ggatttgtaa gctacagata aaatttgcgg agggaatgtg tttatatgaa
     2161 gcggagtgta aagcttaaca gcaactggtt cttttctatg gtggttccgc aggatttaac
     2221 cgccataccc gaaaacgcgg aaagcgttac cgttccccat acttggaata tttacgactt
     2281 aaagcgcagg ggctccaaca caaggggcag atactgctat cagagagagc tgcccgttcc
     2341 gctggagctt tcgggctaca ggctctttct tgaattcaag ggagcgggtc ccttggctga
     2401 gctttacata gacggcaaga aggtgggcac ccatgagggc ggctttacct ccttctgctt
     2461 tgaaatcacc gactttgtaa tccccggcag aaaccatgta atcacaatgc tgaccgacag
     2521 cacagaaagg ggcaaatgct gccctgtaaa tccccatttc gcggttttcg gcggcattta
     2581 cagggatgta agtcttatag tgtcgggcaa gagccatttt gccctgaccg aagacggctc
     2641 taagggcgta ttcatctcca caaagatggc cggctccgac gctcttgtgg ctgttgccgc
     2701 taatatttca aatcccatta actacgatgt tgtcagcttt acgatttacg actccgctga
     2761 gaacgccata ggcacagcga cggctcaccc caagcaggct gaggcgatta tgaggataca
     2821 caatccctct ttgtggaacg gcaccgcttc gccttatctt tacactctca gagctcagct
     2881 gatccgtgac ggcgtgattc ttgaccgtgt ggacattccc ttcggaatca gaaatataga
     2941 gattaagagc agcggctttt atcttaacgg caaaaggaca gagattaagg gcatttccct
     3001 ttatcaggac agggcaaaga cgggaattgc cgcttccgac gagcagatta ggaaagagat
     3061 acaactgtta aaggagctgg gggcaaacgc tgttaggctc aactgcttcc cgcccgatga
     3121 gagcgttctg gactactgtg acagcgaggg catagccgta tggctcgaaa tccccttcgg
     3181 aaacaatata ggaaagaact cccccgcctt taaaaacgcc cgtcagcaat taactgagct
     3241 tataaagcag tactacaacc atccgtcgat tgtggcgtgg ggaattaact gcccgaagtg
     3301 gcacgcgggc gacagctcgg tggcatttgc acaagccgag gatttggcaa ggcttgcaag
     3361 gtctttggac aagacgaggg tttgcgtttt cacagagcag gttgacgccg acacgcccgt
     3421 tttcccgcag agcgcgggcg cgtttaacgc ggacatagat ttgctggacg agttctccgc
     3481 cgaaagggct cttaggactt tggagcttat gagcgaggca aatcccgatt tgccgatttc
     3541 agtatccttc tctacagagc tcagccccga aaagtctgca aatgtccttt tggagaacgc
     3601 cttcaaagcg gcggacgaaa ggggctttat ctggggcgtt ttcgcgggtc ccctctttga
     3661 ctttgctaag cgcacccgtg aaatcggaac agagtttgaa ataaccgagg atattcccgc
     3721 ggaagatgag ccgacagaag aaacctctga ggaaacaaag gcagaggaaa ccgccgaaat
     3781 cgcagctccc gcagaagaaa ccgctgagga gacagaagca gaggaaactc aggaaaccga
     3841 atccgaggaa actgccgaca atgccgagtc agaagaaagc gaaaaggctg aggaagcaga
     3901 ggcagagcct gctgaggaaa gcccagagga agctgccgca agcgaaaagg ctgaggaaga
     3961 aaaagcggag gctacggaaa ggactgcgtc cgcccccgca atcaacggct atgcggagaa
     4021 tattgagatt tcaagctgcg gctttgtaag ctacgacaga cagcaaaaga gggacagctt
     4081 ctaccttatt aaggcgatgt ggtcaaaatc gaagtttatt tacattgccg aggaaaacgg
     4141 ctccttccgc tcaaagagaa agtgcagcat aagagtttat tccaacctta aagacctgcg
     4201 cctgaccgta aacggcaagg agaaaaagag caaaacctta cactccgaaa acggcgtatt
     4261 catctttaac gatgtggttt tggcaaaggg agaaaactca attgtcgcaa gctgcgacta
     4321 cgaaatcgga attaccgaca aggttacaat aatccgccag cgtcaggcag gcaaataaac
     4381 aagaaagagt ggttgcaaaa tgaagatagt ggtcagcgaa aactccaagg ctttgggcgc
     4441 ggcagcggct cagcacatag ccgaaacgat aaataacgcg atagccgaga aaggaaacgc
     4501 gagaattgtg ctctccacag gcgcgtctca gcttgatatg ttcgaggctc ttcttcagct
     4561 caatgttgac tggagcaagg tcgagatgtt ccatcttgac gaatatgtgg gcattgagga
     4621 gactcacaag gcgagcttcc gcaaatattt aaaggagcgt ttcgcatcaa agattaatct
     4681 taaaaacgca tatttccttg acggctccga ggaggtcaca aaattcgtga cagaaaagct
     4741 gcttgaggct gagattgatt tgggcgttat tggaatcggc gagaacgctc acattgcctt
     4801 taacgacccg cccgcagatt ttgacactaa agaggcttat catattgtaa agctggacga
     4861 cgcgtgcaag cgtcagcagg tgcgtgaggg ctggttcccc accgttgagg atgtgccgac
     4921 ccacgctgtt tcaatgacct gctatcagat tttgcagtgc aaaaagatta tctccgcagt
     4981 tccccatgag gttaaggctg aggcggttta caaaacgctg acggagaagg taaacaacaa
     5041 tactcccgcg acacttttaa agacgcaccc taactggacg ctgtatcttg acaaaaactc
     5101 agcgtcaaag gttttccctg tttgagctaa aaaaggaata agatagaagg cttcgggcaa
     5161 aatatccatg gatatgcccg aagctttatt tttgttaaag gggttatgct tatggctgaa
     5221 aacaatcaac atcaggataa ggcatatatg gcgatggctg aaaaagcctc gcccaactcc
     5281 catattttga aaaacgccgt taaggctttt ttgatagggg gtcttatttg cgccttcggt
     5341 caattccttt tctttatcta tgaaaagtgg ggaattgatg aggagacggt aaggtctttg
     5401 gttcccacca cgattattgc cataaccgca gtgctgacgg gactcggctg gtttgacgat
     5461 atagcaaagc acgcaggcgc gggcactctg gttcctatta cgggctttgc gaacgctgtt
     5521 gtctcccccg ccgttgagta tcagacggag ggaagaatcc tcggaacggg cgcaaaaatg
     5581 ttcacaatcg cgggccccgt cattgtttac ggctgcggca cggcgactct ttacggcgta
     5641 atttactata tttgcagtat gtttttaggc gcttgaaagg acttgaaagc atggcacaaa
     5701 gacagggaag atttgtactg aaattaccga ataaacctaa aattctggct accgccgccg
     5761 ttgtgggtcc caaggaagcg gagggtcccc acggtcccga ttttgacaac tgccacagcg
     5821 atgattatgt aggtcaggag acatgggaga aggcggagag cgttattcat aaggaagcca
     5881 tagaaagggc tattaacaag gcgggactga gtccccttga tattgatatg gttttcgcag
     5941 gcgaccttat ggaccaggag atagcctcga ctttagggat aagggagctt aacattccct
     6001 ttgtgggact tttcggcgct tgctccacaa tggctttggg acttgccttg gcgggtctta
     6061 tggcggacac tcatgccgca aactatgcgg ttgcctccac ctcgtcccat ttctgctcgg
     6121 cggaaaagca gttcagatac cccctcgcat acggcgcgca gaggagcccc accgctcaaa
     6181 gaacggttac gggagcgggc gctgctgttg tagccgcaaa cggtcccaag cgggcgccct
     6241 atattgacgc tgttttcctc ggtcgcataa gggatttggg cataaaagac gccgcaaaca
     6301 tgggcgctgc tatggctccc gctgcggcta cgacgattat tgactttatg cacgacaccg
     6361 gcacgaccgc cgacgactac gatttgattc tcacaggcga cttaggaaaa gtaggctcca
     6421 cccttgtcag ggagcttatg ctgacaagag agaacatgga tatttcaaag gttcacaatg
     6481 actgcggcat gatgatattc gaccttgaaa agcaggatgt ccacgcgggc ggctcgggct
     6541 gcggctgctc ggctgctatc ctttgctcat atatctacaa agagcttgaa aagggcaatt
     6601 taaagagcgt tctctttgtg ggcacgggcg cgctgatgtc gcccactttg acccagcagg
     6661 gagaaacaat accctcaata gcccatgcgg tgctgattcg cagcggctgc tagctttaaa
     6721 ggtggtgcgg taatggctaa taacaagatt gcaaaggtaa acggcaataa ggcgctcaca
     6781 tatataagca agacccttga taatattaag gtaatcgaca cagcatccaa gacaattaag
     6841 tttttgtctt atttaaaaat cgccgtagga gcggcagcgg cggctatagt cgtgtatcag
     6901 ctttactccg taatcaagag cgaaatgaaa taacccccca aagtttaagc ggcagggcgt
     6961 atgccctgcc gcttttattt tagattaaag ttctttattc ttattctttt cgccgacctt
     7021 catctgcatc tcggcaagtc tttcaaggtc ctctgctgag cctgaggttg cgctctgtga
     7081 catttcagcg cgtcttgcga ggagctctgc gtacatacgc tctctcttct cgccggccaa
     7141 atcgtagaac agcatcggga ttatgccgag tgatgttgtg gcgaagggaa cgattgtaaa
     7201 cattgcgaac agaccgaatt ttgtatcgtc agactgctgt tttcctgaaa tgtaaagcgt
     7261 ctggtcgtac ttgatgagct ttttgatctg cagctgtacc atgttgctga agagggacgc
     7321 aagctttgta gcaagaccct ttgcaaccga ggtcatagcc tccgtacggt agccgttctt
     7381 ccattcgcag tagtccattg cctcgttgta aagctcgctg gggataacct ttcttacgcc
     7441 gtagaacagc ataaatactg tctcccaaac ggtgagggca attgccatgg gaataatctt
     7501 tttgtaaaga ccgttcttct ttccgccgat agcgcctaca aagaatacgg gcaccataag
     7561 aatgtcgcct atgtatgcgc cgagtatgta aagtgtgcgg ctggagaact ttcttctgaa
     7621 ccacggaaca agcgagtagc ttatcgggtg aacgattgag ccggggagac ctgtaaatgc
     7681 ggcaagggtt gcgaagttga gaacatcgat gaagtagtcc tgc
//
LOCUS       DAONVQ010000096         7626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_7293_length_7626_cov_5.240523, whole genome shotgun sequence.
ACCESSION   DAONVQ010000096 DAONVQ010000000
VERSION     DAONVQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7293_length_7626_cov_5.240523"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PLD98_06760"
     CDS             complement(<1..1150)
                     /locus_tag="PLD98_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQA05982.1"
                     /translation="MRKWSITNRWLLSTLGVTVILMLTIVFVSSKAIRDYYYDTAYMT
                     IYSRATSQSINTFFSQYLDAPDDVFSARAGEFIENFTEQSLMEVWVIDRNGNVVATST
                     GFSVENEIYPDYQAALNSNTGTATWIGETSTGEAIMAVTVLLPSKQGHAVRYIISLQD
                     LNTQHRVFTAIIAVVCTIAVGLVALSGLFFIRSIVKPVKKINETAKRIAAGDFSARLE
                     PHRYNDEIGELVTTFNRMAKEIGEADKLKNDFISTVSHELRTPLTAIKGWGETILELH
                     DSDPNMTKKGLEVIIDESSRLSRMVEDLLDFSRMQSGRMTLRLEKIDVLAELDETVFV
                     FKERALREGVELTYNAPELPAPMMGDPNRIRQVFVNILDNALKYTKQGD"
     gene            complement(1191..1898)
                     /locus_tag="PLD98_06765"
     CDS             complement(1191..1898)
                     /locus_tag="PLD98_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQA05983.1"
                     /translation="MKRILVAEDEASIRDFIVINLQRSGYDVVAVADGEEAIREYEEN
                     EGDFDIALLDIMMPGVDGLEVCKYLRKKSSSIGIVMLTAKTQEMDKVTGLLVGADDYI
                     TKPFSPSELMARIDAIYRRVALNKEAAKASSRQSDEITLGEFTLKIRNRTLTKRGQPI
                     DLTQVEFQIMELFFNNPGAALSRSEILKAVWGDSYYGDEKVVDVNIRRLRMKIEDEPS
                     HPRHLTTIWGIGYKWVV"
     gene            complement(1928..3331)
                     /locus_tag="PLD98_06770"
     CDS             complement(1928..3331)
                     /locus_tag="PLD98_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05984.1"
                     /translation="MEKKIPALIALCLLFCFVLSGCSVGLKAVDNLLRAPKLTGENAD
                     LNMAFEEAARRVRDAGIVLKRPSNGNYRSSFILHDIDGDGTEEAFVFYAFSDDKSATR
                     MNVLSRAGGRWESVSDFPGSGIGVYEILFSDLNGDSIKEIIVAWKISETTTIKHMTIY
                     GGQEEKGLTKDVLKPRANELYSALTVLDIDGDGRNEVLFTHFDTTTERSRAFARVLKM
                     NDKGEIKPAGEAPLNPKISSYTGFKTEYVGELPRVYIDAYASGVQMLTEMLYWDKETG
                     KLVSPFYTSDPAANPVTLRNVIVESSDINQDRNIEIPLTSVLPGSSIIGSDESKSTAL
                     YIVNWSHYENGKFRTVKRTYYNTNDMFSIVYPDEWKNQVTIQQHLTNRLTTFTYYAPG
                     TKRNGLELFSIKAFPVMSFHTDLLGDYEQFLSNKNFTYAYRITEEGRKFGITEDFITS
                     EFNAVKTQSQSSMNIKF"
     gene            complement(3387..4706)
                     /locus_tag="PLD98_06775"
     CDS             complement(3387..4706)
                     /locus_tag="PLD98_06775"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HQA05985.1"
                     /translation="MKNNPSSQKKISNIKKRLSLDTLLSDGKVVLAISFLFAMVLWVL
                     VSMYASPVDIREIKNVKVTLNLEDSLPAKLNLAIFGEKDFYVDVTVRGRRYQLSDSAL
                     AAENLQVVAQMNYVDKAGINTLPLKASFANGGGDLEIISLSRTEIDVYFDTLKTQQYT
                     LEPQIVHDGFEIAQEGFISETPILSSSVIELSGPEMEINKIRNVYAKASIKEPLAANI
                     TLPVSIVPQGDNGEPIKYVESSSDDITVTIPIKKIMTLPTTVSFINAPLAYSEGFLNM
                     RFEPRTVKVAVSTDIADTVDSFPIGIIDFSTLPLGVITREFPPVSTKAITILDEKVKT
                     FRVTIDTSKMREQVFDIPLTNFSAANVPEGRRVTPMDTTLKGVRIIGLENDIASLSAD
                     GIYVESDFSNLEATEGIVTVPVRIFVKSNTSCWAYGDYKIRVRISNS"
     gene            complement(4696..5577)
                     /gene="cdaA"
                     /locus_tag="PLD98_06780"
     CDS             complement(4696..5577)
                     /gene="cdaA"
                     /locus_tag="PLD98_06780"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HQA05986.1"
                     /translation="MLSSAISDLFANVRNVFYGFNLITDVLDILFVTFLIYSLIVQLR
                     KSQSIQVIKGLVLVAVIYAVVHFLEMQTSEYIFNQLFSNAFLIAVIVFAPEIRQALEH
                     VGRSNFGKFSLIAPSKPEAQILDSINAVVRACSSMSRDKIGSLIIFQRKSYLGELTND
                     GVMIDAVTTTDMILSIFYPKSSMHDGAVVIKDGRIVAARCIIPMRNDRELSGDMGTRH
                     RAALEVSIKSDAIAVVTSEETGIISIAVEGQLWRGLKDSELREKLTTLLLPSADSKDT
                     LKDKIRRVFERRKKDEK"
     gene            complement(5617..6099)
                     /gene="ispF"
                     /locus_tag="PLD98_06785"
     CDS             complement(5617..6099)
                     /gene="ispF"
                     /locus_tag="PLD98_06785"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQA05987.1"
                     /translation="MFRIGQGYDVHRFAEGRKLILGGVEVPYEKGLLGHSDADVLCHA
                     VSDALLGAAALGDIGRHFPDSDERYKDADSVELLKKVVALLKDKGYKIVNIDSTVIAQ
                     KPKLKDYIGRMVQNIAAACEIDADCVNVKATTEEGLGFTGALLGIAAQAVCLIEKIND
                     "
     gene            complement(6125..7405)
                     /locus_tag="PLD98_06790"
     CDS             complement(6125..7405)
                     /locus_tag="PLD98_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQA05988.1"
                     /translation="MKALSAYSREELLAFEAAARKQYEEFLSQNLSIDMSRGKPCTEQ
                     LCLSDEILNNVNKDTGFKSANGTECRNYGVPAGIDECRQLFADILEVKPENIFVGGNS
                     SLNLMFDYVAQCMLKGPMGEKGWCYEDEVIFLCPAPGYDRHFSICEYFGIKMVNVPML
                     STGPDMDVVEELVKNPKVKGMFCVPKYSNPMGITFSDETVIRFAKLKPAAKDFRVIWD
                     NAYCVHDLYDEGDKLLNILAEAEKYGNEDHFVVFTSTSKITYPGAGVSALASSVNNIK
                     AIMSRVTMQTIGHDKINQLRHARAFKDLNGIKEHMKLHAKILAPKFEAVLDILSEELE
                     GLEIASWNKPRGGYFISLDIDCGSAKYVGQLCKEAGLVLTPVGATFPYGVDPEDKNIR
                     IAPTFPPVDELKLCCRLLCCCVKLAAAKELLEKA"
BASE COUNT         1738 a         1835 c         1895 g         2158 t
ORIGIN      
        1 tatcaccttg cttggtgtac ttcaaggcat tatccagaat attgacaaag acctgtctta
       61 tgcggttcgg gtcgcccatc atgggagcgg gcagctcggg agcgttgtag gtaagctcca
      121 cgccctctct taaagctctt tccttaaata caaagacggt ttcgtcaagc tcagcgagaa
      181 catctatctt ttcaagcctt aaagtcatcc tgccgctttg cattctggag aagtcgagaa
      241 ggtcctctac cattcttgaa aggcggctgg actcgtcgat tatgacctca aggccctttt
      301 tggtcatatt agggtcggag tcgtgaagct ccagaattgt ctcgccccag cccttaatgg
      361 cggtgagggg ggttctgagc tcatgggaga cggttgagat aaagtcgttt ttcagcttgt
      421 ccgcctcgcc tatctccttt gccatgcggt tgaaggtggt tacaagttcg cctatttcgt
      481 cgttgtacct gtggggctca agccttgcgc tgaaatctcc cgcggcgatt ctcttggcgg
      541 tttcgttaat ctttttaacg ggctttacga ttgagcggat aaagaacaga ccggaaaggg
      601 caactaaacc taccgctatt gtgcatacaa cggcgattat ggcggtgaat accctgtgct
      661 gagtgttaag gtcctgcagg gagattatgt atctgacagc gtgaccctgt tttgaaggca
      721 aaagcaccgt cactgccatt attgcctcgc ccgttgaggt ttcgcctatc catgtcgcgg
      781 tgcccgtgtt ggaattaagg gcggcctgat agtcggggta tatctcattc tctacggaaa
      841 agcctgtgga tgtggcgacc acatttccgt tgcggtcgat tacccagacc tccataaggc
      901 tttgctcggt aaagttttct ataaactcgc ccgctcttgc gctgaacaca tcgtcgggag
      961 cgtcaagata ctggctgaag aaggtgttaa tactctgact cgttgccctt gagtagattg
     1021 tcatgtaggc ggtgtcgtag tagtaatccc ttatagcctt tgagctgaca aagacaatag
     1081 tcagcattaa aataacggta acgccaagcg tactcaaaag ccatctgttt gttatgctcc
     1141 atttgcgcat ggcgttccgt cctttctttc aaagtttaat aagtttgtat ttatactacc
     1201 cacttgtagc cgatgcccca gattgtcgta aggtggcggg ggtgtgaagg ctcgtcttcg
     1261 attttcattc tgaggcgtct tatgtttaca tccacgactt tttcgtcgcc gtagtaggag
     1321 tcgccccaaa ctgctttaag tatttcagac cttgagaggg ccgctccggg gttgttgaaa
     1381 aacagctcca taatctgaaa ttcaacttgt gtaaggtcta tgggctgccc ccgtttggtt
     1441 agtgttctgt ttctgatttt taaggtaaat tcgcccagag ttatctcgtc gctttgtctg
     1501 ctgcttgctt tggcagcctc tttattaagg gcgacgcgcc tgtagatagc gtctattctt
     1561 gccataagct ccgacggtga gaagggcttt gttatgtagt cgtcggcgcc cacaagcaga
     1621 cccgtgacct tgtccatctc ctgagttttt gcggtgagca ttacaatgcc gattgagctg
     1681 cttttctttc ttagatactt gcagacctca agtccgtcaa cgcccggcat cataatgtca
     1741 agcagggcta tgtcaaagtc gccctcgttt tcttcatact ctctgatagc ctcttcaccg
     1801 tctgcaacgg caacgacatc atagccgctc ctttgcagat tgatgacgat aaaatctctt
     1861 atggaggctt cgtcttccgc tacaagtatt cttttcaaag ccctatctcc tttccaagct
     1921 gtcagagcta aaattttata ttcatagatg actgggactg ggttttaacg gcattgaact
     1981 cggaggtgat gaaatcctcg gtaatgccga actttctgcc ctcttcggtg attctgtaag
     2041 cgtaggtaaa gtttttgttg cttaaaaact gctcataatc gccgagcaaa tcggtgtgga
     2101 aggacataac ggggaatgcc ttgattgaga aaagctccag cccgttgcgc tttgtgcccg
     2161 gcgcgtaata tgtgaaggtg gtcagcctgt ttgtaaggtg ctgctgaatg gtgacctgat
     2221 tcttccactc atcgggatag actatcgaga acatatcgtt tgtgttgtaa tatgttcgct
     2281 ttacggtgcg gaatttgccg ttctcatagt gcgaccagtt gacaatgtaa agagctgtgc
     2341 tctttgactc gtcgctgcct ataatgctgc ttccggggag aaccgatgta agggggattt
     2401 caatattgcg gtcctgattt atatcggagc tttccacaat aacatttctg agtgttacgg
     2461 ggtttgcggc ggggtcgctg gtatagaagg gtgaaaccag cttgcccgtt tctttgtccc
     2521 agtagagcat ttcggtgagc atctgaacgc cgctggcgta ggcgtctata taaactcttg
     2581 gcaattcgcc cacatactcg gttttaaagc ctgtatagga gctgattttg gggtttaagg
     2641 gagcttcgcc cgcgggcttt atttcgccct tgtcgttcat tttgagaact ctcgcaaagg
     2701 ctcggctgcg ctcggtcgtg gtgtcaaagt gggtaaaaag cacctcgttg cggccgtcgc
     2761 cgtctatgtc aagcaccgtc agagcggaat aaagctcgtt tgctctcggt ttcaagacat
     2821 ccttagtaag acctttctcc tcctgaccgc cgtagatagt catgtgctta atggtggtgg
     2881 tttcggaaat cttccacgcg actataatct ctttgattga gtcgccgtta aggtcggaga
     2941 aaagaatttc ataaactccg atgcccgaac cggggaagtc ggatacggac tcccatctgc
     3001 cgccggctct tgagagaaca ttcattcttg tggcgctctt atcatcggag aaggcgtaga
     3061 aaacgaacgc ctcctctgtg ccgtcgccgt ctatgtcgtg aaggataaat gaggagcggt
     3121 aattgccgtt tgagggtctt ttaaggacga ttcccgcatc tctgactctg cgggctgcct
     3181 cctcaaaagc catgttaagg tcggcgttct ctccggtaag ctttggggct ctgaggaggt
     3241 tgtctaccgc tttaagacct actgaacagc cgcttaaaac aaaacaaaaa agcaaacaaa
     3301 gggctataag cgcgggtatc ttcttttcca taacagccct cctttgcttt ttgcgtaaat
     3361 atgacttatt gctcaggctc tttggcctat gagtttgaaa ttctgaccct tatcttatag
     3421 tctccgtatg cccagcaact tgtgttgctc tttacaaaga ttcttacggg aacggtgact
     3481 atgccctcag tggcttcaag atttgaaaaa tcgctttcca cataaattcc gtccgcgctg
     3541 agggaggcaa tgtcattttc aagacctatg attctgacgc ccttgagggt tgtgtccatg
     3601 ggggtgactc tcctgccctc cggcacattc gccgcgctga agttggtgag aggtatgtca
     3661 aagacctgct cacgcatctt gcttgtatct attgtaactc taaaggtttt gactttttcg
     3721 tcaagaattg taatcgcctt tgtggatacg ggcgggaatt cccttgtgat aacgcccaga
     3781 ggcagggtgc tgaaatctat aatgcctatg gggaagctgt caaccgtatc cgcaatatca
     3841 gtggagacgg ctactttgac cgttctcggc tcaaagcgca tattaaggaa gccctcgctg
     3901 tacgcaagcg gcgcgtttat aaagctgacc gttgtgggaa gggtcataat cttctttatt
     3961 gggattgtca cagtaatatc gtcggagctg ctctccacat actttatcgg ctccccgttg
     4021 tcgccctgag ggactatgct tacgggcaga gtgatgttcg cggcaagggg ttccttgatg
     4081 gaagcctttg cgtacacatt ccttattttg ttgatttcca tttcgggacc cgaaagctct
     4141 ataacagatg atgaaagtat gggagtttcg ctaataaagc cttcttgcgc tatttcaaag
     4201 ccgtcatgca ctatctgagg ctcaagggtg tactgctgtg ttttgagagt atcgaaataa
     4261 acatcaattt cagtgcggga aaggctgatt atctcaaggt cgccgccgcc gttggcaaag
     4321 ctcgctttta acggcagggt gttaattccc gctttgtcca catagttcat ctgcgccact
     4381 acctgaagat tttctgcggc aagggcggag tcgctgagct gataccttct gcccctgacg
     4441 gttacatcaa catagaaatc cttttcgccg aaaatggcaa gattgagctt tgcgggcagg
     4501 ctgtcctcaa ggtttaaggt gacctttaca ttcttgattt cccttatatc aactgggctt
     4561 gcgtacattg aaacaagcac ccacaaaacc atcgcgaaca aaaacgaaat agccagaaca
     4621 accttgccgt cgctgagaag cgtgtcaagg ctcaggcgtt tctttatatt ggagatcttt
     4681 ttttgtgagg aagggttatt tttcatcctt cttcctcctt tcaaagacac ggcggatttt
     4741 atcttttaag gtgtctttgc tgtcggctga aggcaaaagc agggttgtaa gcttttctct
     4801 cagctcgctg tctttaagac ctctccaaag ctgaccttcc acggcgatgg agataatgcc
     4861 cgtttcctcg gatgtgacta cggcaatggc gtcgctcttg atgctgactt ccaaagcggc
     4921 tctgtgcctt gtgcccatgt cgccggagag ctccctgtcg tttctcatgg ggataatgca
     4981 ccttgcggca acgattctgc cgtcttttat gacaactgcg ccgtcgtgca ttgaggattt
     5041 gggatagaaa atgctgagaa tcatatccgt cgtggtgacc gcgtcaatca ttacgccgtc
     5101 gtttgtaagc tcgcccagat aggatttgcg ctggaaaata atcagagagc cgattttgtc
     5161 gcggctcatt gaggagcatg ccctgacaac ggcgttgatg gagtcaagga tttgcgcctc
     5221 cggcttagag ggggctatca gcgagaattt gccgaagttg cttcttccca catgctccag
     5281 agcttgtcta atctcgggcg cgaagactat aaccgcaatc aaaaaggcgt tggagaagag
     5341 ctggttgaat atatattcgc tcgtctgcat ttcgagaaaa tgcacaacgg cgtaaattac
     5401 ggcaacaaga accagtccct taataacttg tatcgactgg ctctttctga gctgtacgat
     5461 aaggctgtaa atgaggaaag ttacaaagag aatatccaga acatccgtta ttaaattgaa
     5521 gccgtaaaat acattacgca cattggcaaa caaatctgaa attgcagaac tcagcacaga
     5581 caccgccttt aaatttggga atacatgaat ttagatttaa tcatttattt tttcgatgag
     5641 gcaaaccgcc tgagcggcaa tgccgagaag ggcgcccgta aagccgagcc cctcctcggt
     5701 tgtagcttta acatttacgc agtctgcgtc tatttcgcag gccgcggcaa tattttgtac
     5761 cattcggccg atatagtcct tcagcttcgg cttttgcgct ataacggttg agtctatgtt
     5821 aacgatttta tagcctttat cctttaaaag agcgacgact ttctttaaaa gctcgacgct
     5881 gtcggcgtcc ttgtatctct cgtcgctgtc gggaaagtgc ctgcctatgt cgcccaaagc
     5941 ggcggcgccg agaagagcgt cgctgacggc gtggcaaagg acatcggcgt cggagtgacc
     6001 gagaagtccc ttttcataag gaacctcaac tccgccaagg atcagctttc tgccctcggc
     6061 gaacctatgc acatcatagc cctgaccaat cctaaacatt atcggctcct aaatcttcaa
     6121 tactttaagc cttttccaaa agctcttttg cggcggcgag ctttacgcag cagcacagaa
     6181 gtctgcagca gagcttcagc tcgtcaaccg gcgggaaggt gggagcgatt ctgatattct
     6241 tatcctcggg gtcaacgccg taggggaagg tggctcccac gggagtcaga acaagtcctg
     6301 cctccttgca gagctgtccc acatactttg cgctgccgca gtcgatgtcg aggcttatga
     6361 aatatccgcc tctgggcttg ttccatgagg caatctcaag accctcaagc tcctctgaga
     6421 ggatatccag aaccgcctca aacttgggag cgagtatctt tgcgtgaagc ttcatgtgct
     6481 ccttaatgcc gttaaggtcc ttgaacgcgc gggcatgacg gagctgatta atcttatcat
     6541 gtccaattgt ctgcattgta acccttgaca tgatggcttt tatattgttt acggacgagg
     6601 cgagcgccga tacgcccgcg ccggggtatg taatctttga agtggaggta aatacgacga
     6661 aatggtcctc gttgccgtac ttttcggcct cagcgaggat gttaagcagc ttgtcgccct
     6721 cgtcgtaaag gtcgtgaacg cagtaggcgt tgtcccagat tactctgaaa tcctttgcgg
     6781 cgggtttgag ctttgcgaat ctaatgaccg tttcgtctga gaatgtaatg cccatggggt
     6841 ttgaatactt gggtacgcag aacatgccct tgaccttggg gttctttaca agctcctcga
     6901 caacatccat gtcgggaccg gtggagagca tggggacatt aaccatctta atgccaaagt
     6961 attcacagat tgagaaatgc ctgtcgtagc cgggggcggg gcaaaggaaa attacctcgt
     7021 cctcatagca ccagcccttt tcgcccatgg gacccttgag catgcactga gccacatagt
     7081 cgaacattaa gttaaggctg gagtttccgc cgacaaagat attctcgggc tttacttcca
     7141 aaatatcggc aaaaagctgc ctgcactcgt cgatgcctgc gggaacgccg tagtttcggc
     7201 attcggtgcc gttagcgctt ttgaatcccg tatctttgtt gacattgttg aggatttcgt
     7261 cagataggca aagctgctcg gtgcagggct tacctcttga catatcgatg ctgagattct
     7321 gtgagaggaa ttcttcgtac tgctttctcg cagcggcctc aaaggcaagc aattcctcgc
     7381 gtgagtatgc cgataatgct ttcatataaa cctccgtatc taattgtctg tcaaacacat
     7441 attatatcat tatttaatga acgaataaag ctcataataa aatcttttta aaattctccg
     7501 ccgcatctgc ggtgtctacg ctttcgggtc tgtgggcgtc catgcccatg tggaatacca
     7561 ctccgacctc cttgccgatg ttccagattc tccttgcgaa ttcgggatag ccgattaccg
     7621 cgccgg
//
LOCUS       DAONVQ010000097         7609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_7311_length_7609_cov_4.617554, whole genome shotgun sequence.
ACCESSION   DAONVQ010000097 DAONVQ010000000
VERSION     DAONVQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7311_length_7609_cov_4.617554"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLD98_06795"
     CDS             <1..309
                     /locus_tag="PLD98_06795"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF015883.2"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HQA05989.1"
                     /translation="TIHWVNAKDAVDAEVRLYDRLFKVENPADDSDGLSLEDKLNPDS
                     LIILKGCKIEKDILNAKVGQSFQFMRQGYFCIDKDSTEDNLIINRTVQLNSSWDKIAQ
                     "
     gene            321..917
                     /locus_tag="PLD98_06800"
     CDS             321..917
                     /locus_tag="PLD98_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA05990.1"
                     /translation="MKYFYKAVMAVLSLAVFPVFFFLPLIRLKFSGNLAKMINNVLKE
                     PLPNNLSVAKIIDVVNKLKAEYPTIGSAKIWENEKLAPIKTYLIIFIILLAVALILAL
                     LIFIFAVFTKKRLPVTMFSILGLVCSLSLNPVFEAIAKPFMTGKISLGSLIGISLANL
                     LGSVSYLALSAVYFIFILLFGVMTVFNIALKIAEYYSN"
     gene            complement(1001..1116)
                     /gene="rrf"
                     /locus_tag="PLD98_06805"
     rRNA            complement(1001..1116)
                     /gene="rrf"
                     /locus_tag="PLD98_06805"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1236..1739)
                     /locus_tag="PLD98_06810"
     CDS             complement(1236..1739)
                     /locus_tag="PLD98_06810"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HQA05991.1"
                     /translation="MKYKEIDPRELQENPIKMIADDWALLATGNEENHNAMTISWGTI
                     GELWSKEVAIVFVRPTRHTFGLMESSDYFSLNFMGDEHKSAHRVFGSKSGRDMDKFEA
                     TGLKPAYHNGHIYVDEAETVIICKKVAFQDINPAGFIDKGIEDLYPIKDYHRMYVGEI
                     VSVLKKA"
     gene            1846..2529
                     /locus_tag="PLD98_06815"
     CDS             1846..2529
                     /locus_tag="PLD98_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HQA05992.1"
                     /translation="MEEKFVPEIHGYLRKFMLTVPKVISEASGIRIFGKRIKSLVFST
                     DVSIIRNCNADAVIAVYPFTPQPVITQAVIMAADVPVFVGIGGGLTQGIRVLDLGLHA
                     EFQGAIGVVVNAPTSNSTVKQLKEDLDIPIVVTVVSEKEDIAARLEAGAQILNVSAAA
                     KTPELVRKIREQFPKVPIIATGGPTDETILETIRAGANAITWTPPSSGEVFKDIMEAY
                     RRQEGHPPF"
     misc_binding    2588..2829
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2894..3913
                     /gene="pheS"
                     /locus_tag="PLD98_06820"
     CDS             2894..3913
                     /gene="pheS"
                     /locus_tag="PLD98_06820"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQA05993.1"
                     /translation="MKEKIEALRLEAIEALSLAQSLQEIEALRVKFLGKKGELTAVLK
                     MMGSLSAEERPIMGQLANAVRAELEEKIESRKKELKEAEIEKKLVEESIDITMPGNRR
                     RIGHKHPLSIVLDEVKDIFLGMGFKIATGPEIEWDEYNFEALNIPQDHPARDTQDTFY
                     LSENKLLRSQTSPVQIRVMEQQKPPIRVIAPGRVYRSDSVDATHSPLFHQIEGLVVDK
                     GITMATLKGNLADFAKRLYGEDTKIRIRPHHFQFTEPSCEVDVTCFRCHGEGCPMCKG
                     EGFIEILGAGMVHPNVLRRGGIDPEEYSGYAFGMGLERLVMFRFNIDDIRLFYENDLR
                     FLKQF"
     gene            3926..6307
                     /gene="pheT"
                     /locus_tag="PLD98_06825"
     CDS             3926..6307
                     /gene="pheT"
                     /locus_tag="PLD98_06825"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQA05994.1"
                     /translation="MKLSKKWLNDYISLNVTDREFALGMTMSGSKVEGYEKEGEELSN
                     IVVGKILELKKHEDSDHLWVCKVDAGQPELLQIVTGAQNLKVGDYVPVALDNSVVSGG
                     KKIKKGKLRGVLSEGMLCSLEELGLSINDFPYAVEDGIFVLGDDCDKTLGKDIKEAIG
                     LDDTVVEFEITSNRPDCLSVIGLAREASATFGVPYNMPEIKNKGGEGEASNLLKVRID
                     APDKCYRYAGAVVKNVKIEPSPRWMRERLRASGVRPINNIVDITNYVMLEFGQPMHAF
                     DLRYLDGAEVIVRTAKEGEKITTLDGVERELSPEMLVIADANKPVAVAGVMGGEYSGI
                     MDDTTTIVFESACFNGTSVRRTAKKLGLRTEASARFEKELDPNGCLMSLLRALDLVEQ
                     LGAGEVVSGVVDAYPKVKESVRLPFRPDYMNEFIGINVSREQQIKYLESLDIKVEGDE
                     IIIPTFRGDLQQQADIAEEIARLYGYENIPDEPLKGVAVGYLTDYQQFLKDTNNLMLS
                     SGLTEIITYSFISPKAYDKICLPEDSKKRKSIRILNPLGEDTSVMRTTALPSLLTVLS
                     HNYNNRNEEAAVYEIATEYEPWESDDRLATEIPKLMIGLYGANIGYYELKGIIEEYLE
                     KIGVSRYDVRPVKDNPSYHPGRTAEIVVDGKAVCILGEIHPKVLANYEIGTRVYAAEV
                     DLNTVYELRNTERTYKPLPKFPSLKRDIAVVCDKATPVLSLQRAIENAVGKNLESVKL
                     FDVYQGEQIEKDKKSVAFSLTLRAADRTLTDEEADSAVKKALNALSELGAVLR"
     gene            6400..>7609
                     /locus_tag="PLD98_06830"
     CDS             6400..>7609
                     /locus_tag="PLD98_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQA05995.1"
                     /translation="MAKRLVVLFAIFSLMLCSLCMRILQINIGPFSASVSAQSNKRTI
                     SIAESRGAIYDRNGELLVNTKKQIYAAVIPSANVMSQIDNLLTSVDKSLIRKNLERGL
                     PAVYPVGGISGNTPEIKYIATFKRYEENQPAAHLIGYISPEEKKGVSGLEKSFDQLLS
                     SHSGRISVSFGVDGTGKALIGEGITINDENYNSPAGLMLTLDKRLQGIVENALDIYDI
                     EMGAAVVLKAETSEILALASRPVFDPSNPSVSLKAENAPFLNRALMAYSVGSIFKPVV
                     AAAAIENGISESFEHDCVGYVQIGSNRFRCHNRSGHGVLDMAGAIANSCNTYFIELSK
                     QVGAEKVYAMANAFGFGREIELSDGLLSAAGSMPDEAELLSPAALANFSFGQGKLLAT
                     PLQIAAMYAAI"
BASE COUNT         2055 a         1876 c         1878 g         1800 t
ORIGIN      
        1 acaatccact gggtaaacgc aaaagacgcg gtcgacgccg aggttagact ctacgacagg
       61 ctctttaaag tggagaaccc cgccgacgat tccgacggtt tgagtttaga agacaagctc
      121 aaccctgact cccttataat cttaaagggc tgcaagattg aaaaggatat tctgaacgcg
      181 aaggtcggtc agagcttcca gttcatgcgt cagggctatt tctgcatcga caaggacagc
      241 acagaggata atctcataat caaccgcacc gttcaactga attcatcctg ggataaaatt
      301 gcgcagtaga gggtattaag atgaagtact tttataaggc agtaatggct gttttgtcgc
      361 tggcggtttt ccccgtgttc ttctttctgc ctcttataag gcttaaattc agcggcaact
      421 tggccaagat gataaacaat gtattaaagg agcccctgcc caataacctc agcgtcgcga
      481 aaattattga tgtagtaaac aagctgaagg cggagtaccc gaccatcggc tcagcgaaga
      541 tctgggagaa tgagaaactt gccccaatta agacatactt gattatcttt atcattctcc
      601 ttgcggtcgc tctcattttg gctcttttga ttttcatctt cgcggtgttt acaaaaaaga
      661 ggctgcccgt cacaatgttc tcaattttgg gacttgtttg cagcctgagc ttaaatcccg
      721 tgtttgaggc aatagcaaag ccctttatga cgggcaaaat ctcactgggc tcgcttatag
      781 gcatttccct tgcgaatctt cttggctccg tgtcatatct cgctctaagc gcagtctact
      841 tcatctttat cctgcttttc ggtgtcatga ccgtatttaa tattgcgcta aagattgcgg
      901 agtactattc caattaaatt aataaagact gcggaatttc ggttccgcag tcttttttta
      961 gagcaaaaaa aacagacgct attgcgtccg tctttaaaag tgtcggcatc acctattttc
     1021 ccaggcagct tcccgccaag tatcttcggc gcaactgagc ttaactaccg tgttcgggat
     1081 gggaacgggt gggacctcag tgcaatcgac accgactgtg tcacttagtt tcccaagtga
     1141 cgagaaggat tatagcatag ccctttttaa aaatcaatac ctaaaataaa attttttata
     1201 attttatcga aaactcctat actaaaaaat gctatttatg cctttttaag caccgaaacg
     1261 atttctccca catacattct gtgataatcc tttataggat acaaatcctc aatgccttta
     1321 tcaataaagc ccgcagggtt aatatcctgg aacgccacct tcttgcagat aatgaccgtt
     1381 tcggcctcat ccacataaat gtgtccatta tgataagcag gctttaagcc ggtagcctcg
     1441 aatttgtcca tatccctgcc gcttttgctg ccgaaaaccc tatgtgcgct tttgtgctca
     1501 tcgcccataa agtttaatga gaaataatca gagctttcca taagaccgaa ggtatgcctt
     1561 gtgggacgca caaatacaat cgcgacttcc ttgctccaaa gctcaccgat tgttccccag
     1621 ctaattgtca tagcgttgtg gttttcctca ttgcctgtgg ccagcaaagc ccagtcgtcg
     1681 gcaatcatct ttatggggtt ttcttgcagc tctctcggat ctatctcttt atacttcata
     1741 acttccatcc tttcggaatt tgttatataa tatttaacaa atcgacactt tttattaacg
     1801 cttatggtat attataacat aaaaaacaaa aataaaggag ataaaatgga agagaaattt
     1861 gttcccgaaa tccacggata tttaagaaag tttatgctca ccgtgccgaa agtaatctcc
     1921 gaggcatcgg gcataaggat ttttggcaaa agaataaaat cgctggtttt ctccaccgat
     1981 gtcagcataa tcagaaactg caacgcggac gcggtaatag ccgtctatcc ttttactcct
     2041 cagcctgtca tcactcaggc ggttatcatg gccgccgatg tgcccgtatt tgtgggtata
     2101 ggcggaggtc tcacccaggg aatcagagtt cttgacctgg gacttcacgc tgagttccaa
     2161 ggagctatcg gcgttgtagt caacgccccc acctccaaca gcaccgtaaa gcagcttaaa
     2221 gaggaccttg atattccgat tgtcgtcacc gtcgtttccg aaaaagagga catagccgca
     2281 aggcttgagg cgggcgcgca gatacttaat gtatccgctg ccgccaagac tcccgagctt
     2341 gtaagaaaaa tcagggagca gttccccaag gtgccgatta tcgctacggg aggtcccacc
     2401 gacgagacaa tccttgagac catcagagcg ggagcgaacg ccatcacatg gactcctccc
     2461 agcagcggcg aggtctttaa ggacatcatg gaagcttaca gaaggcagga ggggcatccg
     2521 cccttctaat aggctgttga ttttatcttt cggttatgat attataacct aacagaaaac
     2581 ttaaaacgaa aacgctgtga tgaagagagt aggcgtttca aagcaaacac agagagagcg
     2641 ggtaggctga gaaccgcttt ttgcttgggc gctgaagttc acttcggagc ggcacggctg
     2701 aaggcttgcg gcaatccgca ttcatatagg tcgtgacgat tgcccgcgtt aagggctcag
     2761 agtgcccgat actgcgctat ggtgtcggga atccgggtgg taccgcgtgg gttttccctc
     2821 gtcccttttt ggggcgaggg ttttttgatg tacggaattt cttattaaaa taaaaagtaa
     2881 tggggtgtaa gtcttgaaag aaaagataga agcactgagg ctcgaagcta tcgaagcttt
     2941 aagcttagcc caaagccttc aggaaattga ggctctgaga gttaaattcc tcggcaaaaa
     3001 gggcgagctg acagctgttc tcaaaatgat gggctccttg tcggctgagg aaagaccgat
     3061 tatgggccag cttgcgaacg ctgtcagagc ggagctggaa gaaaaaatag aaagcagaaa
     3121 aaaggagctt aaagaggcgg agattgagaa aaagctcgtt gaagaaagca ttgacatcac
     3181 aatgcccggc aaccgccgca gaataggaca caagcatccc ttgtcaatcg tacttgacga
     3241 ggtaaaggat atattcctcg gcatgggctt taagatcgca acaggtcccg aaatagagtg
     3301 ggacgagtac aactttgagg ccctcaacat tccgcaggac caccccgcaa gggacactca
     3361 ggacacattt tatctctccg agaacaaact tctgcgctcc cagacctctc ccgttcagat
     3421 aagggttatg gagcagcaaa agccgccaat cagggttatt gcccccggca gggtttaccg
     3481 ctccgactct gtggatgcca cacactcgcc ccttttccat cagatagagg gacttgtggt
     3541 tgacaagggc atcaccatgg caaccttaaa gggcaatctt gctgactttg cgaagaggct
     3601 ctacggcgag gacacaaaaa taagaattcg tcctcaccat ttccagttca cagagccctc
     3661 ctgcgaggtt gatgtaacct gcttccgctg ccacggcgag ggctgcccca tgtgcaaggg
     3721 agaaggtttt atcgaaatcc ttggcgcggg catggttcac ccgaatgtgc tcagaagggg
     3781 aggcatcgac cctgaggagt acagcggcta cgcgttcggt atgggtcttg agcgccttgt
     3841 catgttccgc tttaacattg atgacatccg tcttttctac gaaaacgacc ttagattctt
     3901 aaagcagttt tagggggtgg actgaatgaa attatcaaaa aaatggctta atgattatat
     3961 atccttaaat gttaccgaca gagagttcgc cctcggcatg accatgagcg gctcaaaggt
     4021 tgagggctac gaaaaagaag gcgaggagtt atcaaacatc gtcgtcggca agattttgga
     4081 gctgaaaaaa cacgaggact ccgaccatct ttgggtctgc aaggttgacg cgggtcagcc
     4141 tgagctcctg caaattgtaa ctggcgctca gaaccttaaa gtgggcgact atgtacccgt
     4201 tgcccttgac aattcagtcg tcagcggcgg caagaagata aagaagggca agctcagggg
     4261 agtactcagc gagggtatgc tctgctcact tgaagagctg ggccttagca taaacgactt
     4321 cccctatgct gtggaggacg gcattttcgt cctcggcgac gactgcgaca agactctcgg
     4381 caaggatatt aaagaggcaa tcggacttga cgatactgtt gtggagttcg agattacttc
     4441 caaccgcccc gactgcctga gcgtaatcgg acttgcaaga gaggcatcgg cgaccttcgg
     4501 cgtgccctat aatatgcccg agattaagaa caagggcgga gagggtgaag cctcaaatct
     4561 cctgaaagtc agaatcgacg ctccggacaa gtgctaccgc tatgcgggcg ctgtcgttaa
     4621 gaatgtaaaa atagagccct ccccccgctg gatgagagaa aggctcaggg catcgggcgt
     4681 ccgtcccatt aacaatatcg ttgacattac taactatgta atgctcgagt tcggtcagcc
     4741 catgcacgcc tttgacttaa gataccttga cggcgcagag gttattgtca gaacggctaa
     4801 agagggcgag aaaatcacaa cccttgacgg cgttgagaga gagcttagcc ctgagatgct
     4861 ggtcatcgca gacgccaata agcccgtcgc tgtagctggc gtaatgggcg gcgagtacag
     4921 cggcattatg gatgacacaa cgacaattgt ctttgagtcc gcctgcttta acggcacgag
     4981 tgtgagaaga accgccaaaa agctgggact ccgcacagag gcctccgcaa gattcgaaaa
     5041 ggagctcgac cccaacggct gccttatgtc cctgctcaga gcccttgacc tggtagagca
     5101 gctcggcgcg ggcgaggttg taagcggcgt ggttgacgct tatcccaagg taaaggagag
     5161 cgtcagactc ccgttccgcc ccgactatat gaacgagttt atcggcatta atgtctcaag
     5221 ggagcagcag ataaaatacc ttgaaagcct tgatattaaa gttgagggcg atgaaataat
     5281 catccccacc ttcagaggcg acctgcagca gcaggcggat attgccgagg aaatagcaag
     5341 gctctacggc tatgagaata tccccgacga gcccttaaag ggagtcgccg tcggctatct
     5401 taccgattat cagcagttct taaaagatac caacaatctc atgctgtcgt ccggtcttac
     5461 cgaaataata acctactcct tcatctcacc caaggcatat gataagatat gcctgcccga
     5521 ggacagcaaa aagagaaaga gcataagaat tctcaatcct ttgggcgagg acaccagcgt
     5581 tatgaggact acggctctgc ccagcctcct caccgttctc tctcacaact acaacaaccg
     5641 caacgaggag gcggcggttt acgaaatcgc caccgagtac gagccgtggg aaagcgacga
     5701 ccggcttgcg accgaaattc ctaagctcat gataggtctt tacggcgcca atatcggcta
     5761 ctatgagctc aagggcataa tcgaggagta ccttgagaaa ataggcgtaa gcagatacga
     5821 tgtccgcccc gtaaaggata acccctcata tcaccccggc agaacagccg agattgtggt
     5881 tgacggcaaa gcagtatgca ttctgggcga aatccacccg aaggtgctgg caaactacga
     5941 aatcggcaca agggtttacg ctgctgaggt tgaccttaac actgtttacg agctgagaaa
     6001 caccgagcgc acctacaagc cgctgcccaa gttcccgtca ttaaagaggg atatcgctgt
     6061 cgtctgcgac aaggcaacac ccgttctgag tctgcagaga gcaatcgaga acgcagtggg
     6121 caagaacctt gaaagcgtaa agctcttcga tgtctatcag ggcgagcaga tagaaaagga
     6181 caaaaagagc gtcgctttta gccttactct cagagcagcc gacaggacac tgacggatga
     6241 ggaggcagac tcggcagtca agaaggcact caacgccctg agtgagctgg gcgccgttct
     6301 cagataattt catcaaagta tagaaaatct ttaaatgtca ataataaata ccgatgaagc
     6361 gtttgctttg tcggtatttt tctttgtggg ggatgcgccg tggctaaaag gcttgttgtc
     6421 ttgttcgcaa tcttttccct tatgctctgc agcctttgca tgaggattct tcaaatcaac
     6481 atagggccct tcagcgcctc ggtttccgcc cagtccaaca aaaggacaat aagcatcgcg
     6541 gaaagcaggg gagccattta cgaccgcaac ggcgagcttt tagtcaacac caaaaagcag
     6601 atttacgccg ccgttatccc ctccgctaat gtaatgagcc agatagataa cctccttaca
     6661 agcgttgaca aaagccttat cagaaagaac cttgaaaggg ggctgcccgc cgtttacccc
     6721 gtgggcggaa tcagcggcaa cacgcccgag attaagtaca tcgccacctt taaacgctat
     6781 gaggaaaacc agcccgcggc tcatcttatt ggctatataa gccccgagga gaaaaagggc
     6841 gtcagcggcc ttgaaaagag ctttgaccag cttctatcct cccattcggg caggatttcc
     6901 gtcagcttcg gcgttgacgg tacaggcaaa gcccttatag gcgagggaat cacaatcaac
     6961 gacgaaaact acaattcccc cgcgggactg atgctcactc tggacaaacg ccttcagggt
     7021 attgtggaaa acgcccttga catatatgac attgaaatgg gagcggcagt ggttttaaag
     7081 gcggaaacca gcgaaatcct tgcccttgcc agccgccccg tatttgaccc aagcaacccc
     7141 tccgtgtcct taaaagctga aaacgccccg tttttaaaca gggcgcttat ggcttattct
     7201 gtaggttcaa tttttaagcc cgtggttgcc gccgccgcta ttgaaaacgg catcagcgag
     7261 agttttgagc atgattgtgt gggctatgtg caaataggca gcaaccgatt cagatgccac
     7321 aaccgcagcg gtcacggtgt gcttgacatg gcgggcgcca ttgcaaactc ctgcaacacc
     7381 tactttattg agctttcaaa acaggtggga gcggagaaag tatacgcaat ggcaaacgcc
     7441 ttcggattcg gcagggaaat tgagctttcc gacggtcttt tatccgccgc aggcagtatg
     7501 cccgatgagg ctgaacttct cagccccgcg gcgctcgcaa acttcagctt cggtcagggc
     7561 aagctgcttg ccacgcccct tcagattgcc gccatgtatg ccgcaatcg
//
LOCUS       DAONVQ010000098         7468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_7486_length_7468_cov_4.709699, whole genome shotgun sequence.
ACCESSION   DAONVQ010000098 DAONVQ010000000
VERSION     DAONVQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7486_length_7468_cov_4.709699"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1163
                     /locus_tag="PLD98_06835"
     CDS             114..1163
                     /locus_tag="PLD98_06835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQA05996.1"
                     /translation="MKKIAIFQTDLLVGGIQKSLLNFLNRFGGKDYLIDVYLFNEEKF
                     YDVAKLPENIRFIYLKPFNYFNRLVYFDILRRFKKYDIKEQYDIAIDFNSYSNECAIG
                     ALCAKANKRIIWIHNDVRIKMSEEPKYRLLFHFFKGKFKYFDAFAAVSDGIIEPFSLE
                     TGIEKERIFTVNNFIDTDEIFRKSEERIDFTVDSSKYNLASMGRLCHQKGFDLLLNKF
                     KDVIATRKDMHLYIIGDGPDRESLERQTKENGLTDFVTFLGNQKNPFPYLNQMDGFVL
                     DSRYEGQGIVIWEAKALGLEIFIPKHLEKYNKNIEGYEDLTSAIINAKKREKQRDPLI
                     EYNEDIMKSLEALFN"
     gene            1193..2254
                     /locus_tag="PLD98_06840"
     CDS             1193..2254
                     /locus_tag="PLD98_06840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQA05997.1"
                     /translation="MPLLSVIMPAYNAAAYIEKAIDSVLSQSFKDFELIVVNDGSADN
                     TLDIINSYKDERLKVYTKPNSGPADTRNWGIERSSGEFIMFIDSDDSFSEGAFETIAR
                     HCGEGSFDMLIFGFVQHNVETGSSFKYFLPDMEAAVKQDLKDKFGDLYVSNLLNSVWN
                     KVFRAETLKENSIKFCDYLYGEDRLFVFDFLRHCDRIKVISDCLYNYIMMNKNSLVSR
                     YYDKKFEACCAINKAIYALQEVTGSFSEETLNNIRYMFLKSVISCITNIYHPSCKKTA
                     SEKRGELKEIINHSDVVNALTEYKSSDIVMDMLVIILKSKSIPLISMASKGIIFTSKN
                     LSAPFVKLKNYKILKSRAE"
     gene            complement(2310..2564)
                     /locus_tag="PLD98_06845"
     CDS             complement(2310..2564)
                     /locus_tag="PLD98_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HQA05998.1"
                     /translation="MKSTGIVRRIDDAGRIVVPMELRRNMNIKDSEDFLEIFVDDDKI
                     ILKKYMPSCIFCKSLDDVVEHQGIKVCKSCINALNSKISE"
     gene            3125..3709
                     /locus_tag="PLD98_06850"
     CDS             3125..3709
                     /locus_tag="PLD98_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HQA05999.1"
                     /translation="MISYVWLSLIVFSFIAAIINGTFYELSASIISSGTDAVQLCIKL
                     LGMLCLWSGLMNIAEKSGLTNLISKLFSPLFSLLFPRLKNNKHIMGPVSMNITANLLG
                     LGNAATPLGIEAMKRMKEVSPLDRTASNEMIRFVVLNTAAIHIFPTTVAVLRQSYGSA
                     NPFDIVIPAWISSMVTLSCGLLMTRLLERERWKR"
     gene            3697..4218
                     /locus_tag="PLD98_06855"
     CDS             3697..4218
                     /locus_tag="PLD98_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HQA06000.1"
                     /translation="MEKISNLILPVIVVVIVVFGAVKKINVFDCFIEGAKKGLVTFTN
                     ILPVLTALIVAVGMMRGSGGLDLITKIFSPIAKLLGIPIQVLPLCIISPISGSGSIAV
                     FEDILRNYGPDSIAGRVASVIAGSSETTLYTTTVYYGSVGITRTRHTIPSALIADLVN
                     FVISSLIVKITFA"
     gene            4625..5194
                     /locus_tag="PLD98_06860"
     CDS             4625..5194
                     /locus_tag="PLD98_06860"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004444058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HQA06001.1"
                     /translation="MDKLKEKILTEGKVLPGNILKVDMFLNHQVDYQLLKEAGLEFYN
                     FFKDCGVNKILTIEASGIAVACAVADAFKVPFVFAKKGSHSNVGSNVYTSEVFSYTKG
                     KSYTLTVSKDYINKDDRILIVDDFLANGEAVLGLKRIIDEAGGELCGVGIIIEKRFQN
                     GYKKLVDAGIKLKSLAVIESMNDGNIVFG"
     gene            5233..6408
                     /locus_tag="PLD98_06865"
     CDS             5233..6408
                     /locus_tag="PLD98_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQA06002.1"
                     /translation="MKKSVRIIALILALATVFALVGCGKQTGGEGETQDTTLAEGQTF
                     APIPADKIKIGVVHITSKDDTSGYTYAHQKGIEEMAAALGLKSEQIVIRDNVDDNDVE
                     ATKAALQDLVDAGCDVIFATSYNYMDAVEEYAEKYPDIIFSHCSGYKSNGKNFNNYFG
                     RIYEARYLSGIAAGLKAKELGVPIVGFVGAKGSDNSEVTGGINAFALGVQSVYPECEV
                     LVKITGEWYDPAAEGIAAKALIDAGAVVLSQHCDSDTVQTTAAQAGRFGVGYNSDMTP
                     SAPNNHLTAPIWNWGVYYTQTVEAIIKGEWKCENYYEGYNVNLVGLSPLNEKAIAPGT
                     KEAVEEAQKKIASGELFVFSGPLYDNQGNQILKEGEKLTDEQITGEINYYIKGVRVL"
     gene            6606..>7468
                     /locus_tag="PLD98_06870"
     CDS             6606..>7468
                     /locus_tag="PLD98_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA06003.1"
                     /translation="MTNTTYAIELKNICKNFGSVAANKNVSLNVKYGEILALLGENGS
                     GKTTLMNMLSGIYHPDSGEIWIDGKHTVINSPKDSFKLKIGMIHQHFKLVDVFTAAEN
                     IIMGTEGFVGSSKKAAEKIKEIADGFGFSIDPNKKVYDMSVGEKQTVEIIKVLYRGAR
                     ILILDEPTAVLTPQETEKLFKVLDNMRKDGCAIIIITHKLNEVMAISDRVTVLRKGET
                     VATVDTKTTDIRKLTELMVGRPVELKIDRPETERSAEPMFFVKDLCVRNEEDVFALKN
                     VSFELYGGEILG"
BASE COUNT         2267 a         1479 c         1622 g         2100 t
ORIGIN      
        1 gagatactta aggaaatcag tttggcggac ctggcaaaac atgcgaacga ttacggactt
       61 aacggggaat acatgttctt tatttaagaa ctcatttaaa aggaatgaca agcttgaaga
      121 aaatcgcaat tttccaaaca gaccttttgg tcggcggaat tcaaaagtca ctgctaaact
      181 ttcttaatag gttcggcggt aaggattatc ttattgatgt ctatttgttc aatgaggaaa
      241 agttttatga tgtcgcgaag ctccctgaga atataaggtt tatatattta aagcccttta
      301 attactttaa caggctcgta tattttgata ttctcaggag attcaagaaa tatgatataa
      361 aagagcagta cgatatagca atcgatttta acagctacag caacgagtgc gccataggag
      421 ctttgtgcgc aaaggccaat aagcgcatta tatggataca taatgatgtg cgcattaaga
      481 tgagcgagga gccgaaatac agacttctct ttcatttttt taaaggcaaa ttcaaatact
      541 ttgacgcctt tgccgctgtt tccgacggta taattgagcc cttttcatta gaaacaggca
      601 ttgaaaaaga gcgtattttc acggtaaata actttattga caccgatgaa atcttcagaa
      661 aaagcgaaga aagaattgac tttactgttg attcatcaaa gtacaatctt gcgtccatgg
      721 gcaggctatg ccatcaaaag ggctttgatt tactgcttaa taagttcaaa gatgtaatcg
      781 ccacccgaaa ggacatgcat ttatacatta tcggcgacgg tcccgacagg gagagtcttg
      841 aaagacagac taaagaaaac ggtcttacgg atttcgttac ctttctcgga aatcaaaaaa
      901 acccctttcc gtacttaaat caaatggacg gctttgtcct tgattcaaga tacgaaggtc
      961 agggtatagt catttgggag gcaaaggcac tcggccttga aatatttatc ccgaagcatc
     1021 ttgagaaata caacaaaaac attgaaggct atgaggattt aacctccgcg ataatcaatg
     1081 cgaagaaaag ggagaagcaa agggacccct taatcgaata caacgaggat ataatgaaaa
     1141 gccttgaggc tctttttaac taattctctg ttctcttaca ggaggaaatt tgatgcccct
     1201 actcagcgta ataatgcccg cgtacaacgc ggcggcatat atagaaaaag ccattgacag
     1261 cgttctctct cagtccttta aagactttga gctgattgtt gtcaatgacg gctctgccga
     1321 caatacactc gatattataa actcttataa agatgaaagg ctgaaggttt ataccaagcc
     1381 caactccgga cccgccgaca caaggaattg gggaattgag agatcttccg gagagtttat
     1441 aatgtttatt gacagcgacg acagcttttc ggaaggcgcg tttgaaacaa tcgcccgcca
     1501 ctgcggagag ggcagctttg atatgctgat tttcggcttt gttcagcaca atgtggagac
     1561 gggcagcagc tttaaatact tccttcccga tatggaagct gccgtaaagc aggatttaaa
     1621 ggacaagttc ggcgacctat atgtcagcaa tcttttgaac tccgtatgga ataaggtttt
     1681 cagagctgaa accttgaagg agaattccat taagttctgt gattacttat acggagagga
     1741 caggctattt gtttttgatt ttctccgcca ttgcgacaga ataaaagtca ttagcgactg
     1801 tctttacaac tacataatga tgaacaagaa cagccttgtt tcaagatatt acgataaaaa
     1861 gtttgaggca tgctgtgcga taaacaaggc tatatatgca ctccaggagg ttacgggcag
     1921 cttttcggaa gagacactga acaatatcag atacatgttc ctgaaaagcg tcatttcctg
     1981 cattacaaac atttatcatc catcctgcaa gaaaactgcg tcagagaaaa ggggagagct
     2041 taaagaaatc attaatcatt cggatgttgt aaatgcgcta actgagtata aaagctctga
     2101 tatcgttatg gatatgcttg tcataattct caagagcaaa agcatacccc ttatttccat
     2161 ggcgtcgaaa ggtattattt ttacaagcaa aaatctaagc gccccctttg taaagctaaa
     2221 gaattataaa atacttaaaa gcagagcaga gtagcaggca actaaaaaag tccacctgaa
     2281 aaggtggact ttgttttcta tgtaattaat tactcagaga ttttggaatt gagtgcgttt
     2341 atgcagctct tgcatacctt aatgccctga tgttcaacta catcgtcaag gctcttgcag
     2401 aaaatgcatg acggcatgta tttcttaaga attatcttat cgtcatcgac gaatatctca
     2461 agaaaatctt cagaatcttt tatattcatg ttccttctta attccatcgg aacaacgatt
     2521 ctgcctgcgt cgtcaatgcg tctgacgata ccggtagatt tcatattttc ctcctccttg
     2581 catttattgg tgactacaaa ctaaagatac caaataatat attatatgtc aatattttta
     2641 agaaaaaatg ttcaaaaaaa ttcatttttt tcaatcaaaa ataaaaatcc cgaattgact
     2701 gtcgaataat gtcgacaaat gcagtccggg aaaaaaggcc actgaaaatc taataaaatc
     2761 aagcgattag aagaggttgt atgtgaggcc actgtttcaa atttgctgtc gaataatgtc
     2821 agcaaaaaaa ttaaatttaa aatagccatt gcgaacgaac ttgcaaatcg acaaaatacg
     2881 acatcaaagt ggcgtcaaaa ttcttcccct gggcatatag agtgtctcaa tttcactcca
     2941 gtgaattata ccatttaatt acattgcagt caatgtaaaa aaatttatat aatgacaaat
     3001 tttgctatta attttttaga aaattgtgca aatttacgag ttcggcgcaa gcgttgttgg
     3061 aacttgaaaa aaagcaaaaa ctcttgcctg tcatacttgt ctaaaaacag aatataaatg
     3121 tagcatgatt agttatgttt ggctgtcgct gattgtattt tcatttattg ctgcaataat
     3181 aaacggtaca ttttatgaat tgtccgcttc aattatcagc agcggtacgg acgcggttca
     3241 gctttgcatt aagctgctgg ggatgctctg cctttggagc ggtctgatga acattgccga
     3301 gaaatcaggg cttacaaatc ttatatcaaa gctgttttcg ccgctgtttt ctcttctctt
     3361 tccgcgcctc aaaaataata agcatataat gggcccggtg tcaatgaata ttacggcaaa
     3421 tttattggga ctaggaaatg ccgcaacgcc tttgggaatt gaggctatga agagaatgaa
     3481 ggaggtaagt cctctagacc gaacggcaag caatgagatg attagattcg tcgtgcttaa
     3541 tactgccgca atacatattt tcccgaccac cgttgcggta ttaaggcaat catacggctc
     3601 cgcaaatccc tttgacattg tcatacctgc ttggatttca tcgatggtaa ctcttagctg
     3661 cgggctttta atgacacgcc ttttggagag agaaagatgg aaaagataag caatttaata
     3721 cttcctgtaa ttgtggttgt aatagttgta ttcggcgctg ttaagaagat taatgtattt
     3781 gactgcttta ttgagggagc gaaaaagggt cttgtgacct ttactaatat attgccagtg
     3841 ttgacagcgc tgattgtggc agtgggcatg atgagaggtt ccggggggct ggaccttatt
     3901 acaaaaatat tctcgccgat tgcaaagctg cttggcatac cgattcaggt gctgcccctg
     3961 tgcattatta gcccaatatc gggcagcggc tcaattgcag tctttgagga tattctcaga
     4021 aactacggac ccgattcaat agcgggcagg gtagcctccg ttattgcggg ctccagcgaa
     4081 accacccttt ataccacaac cgtatattac ggctcagttg ggataacgcg cacccgccac
     4141 actattccca gcgctttgat tgcggacctg gtgaacttcg ttatttcctc attaattgta
     4201 aagattacct tcgcctgagc gggatttttt ggagttaatg acggcgtctt atttgtgcaa
     4261 tctgcacaat cgctgcttcc gttccagttt ctggtaattc gttaaatttt aaaattttgt
     4321 atagaatgca caaatggagc gatttttgag ctcgcaacac aaatatctaa atttgcaaat
     4381 cgagaatact tatctatcat agaaagctca gagtttcaac gaatttttga gcttttttct
     4441 tttttgcagc acaattcaga tcagtattct ctatgccttt acagtgctaa tgcgttaatg
     4501 tgtttttgtt caaaacgcag aaaaggctta tcttgcctta attcaattga tttttaactt
     4561 gaattcaaat ataatgttgc tgtcgaaatt cgaagtgttt tgcagtttct gttggaggcc
     4621 ttaaatggac aagctgaaag aaaaaatcct gactgaagga aaggtccttc cgggaaatat
     4681 cctaaaagtt gatatgtttc ttaaccatca agttgattat cagcttttaa aagaggcggg
     4741 cctcgagttt tataacttct ttaaagattg cggagtaaat aagattctga ctattgaagc
     4801 ttcaggaatt gcggtggcat gtgctgttgc cgacgcgttt aaagtaccct ttgtgttcgc
     4861 aaagaagggc agccattcaa atgtaggcag caatgtttac acctctgaag ttttctctta
     4921 tacaaaagga aagtcttata cccttacggt ttcaaaggat tacataaata aagacgatag
     4981 aatccttatt gttgatgatt ttctcgcaaa cggagaggca gttctcggtt taaaaagaat
     5041 tattgatgaa gcgggaggtg agctgtgcgg tgtcggcata ataattgaaa agcgtttcca
     5101 gaacggatac aaaaagctcg tggatgccgg tataaaactt aagtccttag cagtcatcga
     5161 gtcaatgaat gatggaaaca ttgtttttgg ctgattgact gttggaatac aaatataacg
     5221 gaggaatgta aattgaagaa gtcggttagg attattgctc ttatccttgc gcttgcaacc
     5281 gtatttgccc ttgttggctg cggcaagcag acaggcggag aaggcgaaac tcaggacaca
     5341 acccttgctg agggccagac ctttgctccc attcccgctg acaagattaa gattggcgta
     5401 gttcacatta caagcaagga cgatacatca ggctatacat atgctcatca gaagggcatc
     5461 gaagaaatgg ccgcagctct tggacttaag agcgaacaga ttgtcatcag agacaatgtt
     5521 gacgacaacg atgtagaggc tacaaaagct gctcttcagg accttgttga cgctggctgc
     5581 gatgtaatct tcgcaacaag ctacaactac atggacgctg ttgaagaata tgctgagaaa
     5641 tatccggata tcatattctc acactgctca ggctataaga gcaacggcaa gaacttcaac
     5701 aactatttcg gcagaattta tgaggctcgt tacctttccg gtatagctgc aggtctcaag
     5761 gctaaggagc ttggcgttcc gattgttggc ttcgttggcg ctaagggatc tgacaactcc
     5821 gaagtaaccg gcggtatcaa cgcatttgct ctcggcgtac agtctgttta tcccgagtgc
     5881 gaagttcttg taaagattac aggcgagtgg tatgatcccg ctgctgaagg cattgctgct
     5941 aaggctctta tcgacgcagg cgcagtagtt ctttctcagc actgcgactc cgacaccgtt
     6001 cagactacag ctgctcaggc tggcaggttc ggtgttggct acaactccga tatgactcct
     6061 tccgctccca acaatcacct cacagctccc atctggaact ggggcgtata ctacactcag
     6121 actgttgaag ctatcatcaa gggtgaatgg aagtgcgaga actactatga gggttacaat
     6181 gtaaaccttg taggtctttc acctctcaat gagaaggcta tagctcccgg aaccaaggaa
     6241 gctgttgaag aggctcagaa gaagattgca agcggcgagc tcttcgtatt cagcggcccg
     6301 ctctatgaca atcagggcaa ccagattctc aaggaaggcg agaagctcac agacgagcag
     6361 atcaccggcg aaatcaacta ctacatcaag ggcgtaagag ttctgtaagc ctaattagca
     6421 ttaataaggg acacatcgtt cggtgtgtcc ctgtttttta gttatcctaa tttctgtttt
     6481 ttccgcaaga ccggtttctt cagaaaatta tttaatttaa tagacaaaaa aggacataat
     6541 ccattataat aattctatta gtggcaagac accatagagc tatatcttta gagaggggaa
     6601 aggttttgac aaacactacc tacgccattg agttaaagaa catatgcaaa aacttcggca
     6661 gtgttgcggc taacaaaaat gtttcgctga atgttaaata cggcgaaata cttgccttgc
     6721 tcggcgaaaa cggctcaggc aaaaccacgc tgatgaacat gctctcgggc atatatcatc
     6781 ccgacagcgg cgagatttgg attgacggaa agcatactgt catcaattca cccaaagact
     6841 cctttaaact taaaatcggt atgattcatc agcactttaa actcgttgat gtttttaccg
     6901 ctgccgaaaa tataataatg ggcactgagg gctttgtcgg cagcagcaaa aaggcggctg
     6961 aaaagataaa agaaatcgcc gacggcttcg gtttttcaat agaccccaac aaaaaggttt
     7021 acgatatgtc tgttggcgaa aagcagactg tcgagattat aaaggtatta tacagaggtg
     7081 cccgcatact tattctggac gagcctacgg cagttctgac ccctcaagaa accgaaaagc
     7141 tgtttaaggt ccttgacaat atgcgcaagg acgggtgcgc tattatcata atcactcata
     7201 aattgaatga ggttatggca ataagcgacc gtgtaacggt tctcagaaag ggcgagaccg
     7261 tagcgaccgt tgacaccaaa acaacggata tcaggaaact tactgagctt atggtcggcc
     7321 gccccgttga actgaagatt gacaggcccg aaaccgaaag gtctgctgag cctatgttct
     7381 tcgtaaaaga cctttgcgtc agaaacgaag aagatgtttt tgctcttaaa aatgtctcct
     7441 ttgagctcta tggcggcgaa attctcgg
//
LOCUS       DAONVQ010000099         7181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_7888_length_7181_cov_5.017541, whole genome shotgun sequence.
ACCESSION   DAONVQ010000099 DAONVQ010000000
VERSION     DAONVQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7888_length_7181_cov_5.017541"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PLD98_06875"
     CDS             complement(<1..1172)
                     /locus_tag="PLD98_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HQA06004.1"
                     /translation="MEFKSFSEKQLKVLSWWSEGSPYKDFDAIICDGAVRSGKTICMG
                     LSFVIWAMTGFSGISFALCGKTIASLRRNVLVPLLPVLKELGFACTEKLSQNLVEIEF
                     NGNKNRFYVFGGRDESSAALIQGMTLGGVMLDEVALMPRSFVEQALARCSVKGSKMWF
                     NCNPENPQHWFHTEWILKAKEKNCLYLHFTMEDNPSLSPAIIKRYKSLYSGAFYERFV
                     EGKWVAAQGLVYPFFSESACVALPKGECRSFYISCDYGTVNPASFGLWGEYRGVWYRI
                     DEFYFDSRAEGYQKTDEEYYADLEKLAGDRDIEAVIVDPSAVSFIECIRRKGKFNVIP
                     AKNDVLDGIRRAAEAIKSGKIKIAPACKDIIREFGLYRWDSASARDSVRKEHDHAM"
     gene            complement(1501..1977)
                     /locus_tag="PLD98_06880"
     CDS             complement(1501..1977)
                     /locus_tag="PLD98_06880"
                     /inference="COORDINATES: protein motif:HMM:NF015550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HQA06005.1"
                     /translation="MRKELSRKERLFCDYYSKTRNSREAAFKAGYRILPERAAEKLMS
                     RKDIREMISQLDEMRKAKTADISAGLRRAAFGSIADAIRLMFDENLNPEELDGLDLFC
                     VSEIKRPKGGGMEIKFIDRLKALEKLQSLAETEREADKNPFVEAIEKSAKAVFSGE"
     gene            2284..4209
                     /gene="glgB"
                     /locus_tag="PLD98_06885"
     CDS             2284..4209
                     /gene="glgB"
                     /locus_tag="PLD98_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HQA06006.1"
                     /translation="MKYADLSELNEALNRFVSGDAEDAYEFMGSHFLSDDTAVFRVWA
                     PNAERVSLCGDFNEWNKEAHPLDPIGFGVWEIILEGVRLYDNYKYCIYTRDGRQILKS
                     DPYAFHSETRPATASKLYKLGGYTWTDDEWLKKRKAEPIYSKPVNIYELHAGSWKRHE
                     DGSFYSYRELADSLVPYILEMGYTHVELLPLAEHPLDGSWGYQVTGYYSVTSRYGTPE
                     DFMYFVDKCHNAGIGVILDWVPAHFPRDEHGLREFDGGPLYEYADPRKGGHPQWGTCV
                     FDYGRNEVKSFLISNALFWFKEYHIDGLRVDAVASMLYLDYCRGEGQWTPNIYGGNEN
                     LEAVAFLQKLNTAVFAHFPDVMMIAEESTAWPLVTKPVHCGGLGFNFKWNMGWMNDIL
                     RYFSLDPYFRKYNHDLITFSFFYAFSENFILPISHDEVVHGKKSLLDKMPGTYDEKFA
                     SVRAFMGYAYAHPGKKLIFMGQEFGHFIEWDEKKQLDWFLLNYDRHRQLKEYVAALNR
                     FYKENPPLWQIDFSWDGFKWIVSDDIDNSVLVFRRINEDDNEIIAVLNFTPVKRDIYR
                     FGVPEKGVYDIVFNSDEERFGGTGCLKKTSFKAEKYSMHDLRYSIEITVPPLSALFLK
                     KRETKKENNKSEKTRAN"
     gene            4249..5463
                     /locus_tag="PLD98_06890"
     CDS             4249..5463
                     /locus_tag="PLD98_06890"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HQA06007.1"
                     /translation="MPRKQECIAMLLAGGQGSRLGILTKNIAKPAVPYGGKYRIIDFP
                     LSNCVNSGIYTVGVLTQYQPLELNDYIGNGQPWDLDRAEGGVHILPPYQQIKGSEWYK
                     GTANAIYQNIHFIDRYSPDYVAILSGDHIYKMDYDKMLSYHKEKNADCTIACIEVPWE
                     EASRFGLMFVDSEGRITDFEEKPKNPRSNKASMGVYIFTWSKLRQYLIDDEADPNSSN
                     DFGKDVIPKMHRAGERMYAYEFDGYWKDVGTIDSLWEANLDLLNPQINIDLDDASWKI
                     YSRNAAAPPHYISADSKVENSLVSAGCEIEGEIDYSVLFSNVTVEKGACVKYSIIMPG
                     AVIKEGAVVQYSIVAENAVIESGAVVGEKPESMKDPSAWGVAVVGAGVTIGKGVTVPS
                     KAMIDKDMKGGN"
     gene            5465..6580
                     /gene="glgD"
                     /locus_tag="PLD98_06895"
     CDS             5465..6580
                     /gene="glgD"
                     /locus_tag="PLD98_06895"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HQA06008.1"
                     /translation="MRARNILGIIFANMYDDCLHELTALRTMASVPFGGRYRLIDFHL
                     SNMVNCGIDKVGVVTKGNYRSLMDHLGNGRPWDLARKRDGLFLLPPFNADSSRSRSSR
                     LEALIDLLPFIESADDEFVILTDCNVVCSFDYSGLIDFHKNTGADITVCCKRGIRPNL
                     NDLMTFETDSDCRVTKIAVSPKLTEECLYSLNIVLLRKPFLERLIAEAKSLNYRSFTR
                     DIMQSNVEKFKIYAYEPEGFIRTIDSLKSYYDISMELLKRENRKELFSADRPVFTKVR
                     DDMPAIYGINCKAKNSLIADGCHIEGEVENCILFRGARVGKNAVVKNSILMQGTIVSE
                     GAHLNCVITDKNVTVTQGKNISGAESFPIYISKGIRV"
     gene            6601..>7181
                     /locus_tag="PLD98_06900"
     CDS             6601..>7181
                     /locus_tag="PLD98_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HQA06009.1"
                     /translation="MKVLYAVSEARPFAASGGLADVAGSLPQALRKRLVGCRVVMPLY
                     ASVPEELRQNMKFITHISVPVSWRSQYCGIFEARHGGVIYYLLDNQYYFKRDNYYSYY
                     DDAERFAFFARAVLEIIPHIGFVPDIIHCNDWQTALTPVYHSCFYANKEGYSHIKTIF
                     TIHNIQYQGVFGREILHDVLGLGDEHYSLLEYDG"
BASE COUNT         1764 a         1926 c         1818 g         1673 t
ORIGIN      
        1 tccatggcgt ggtcgtgctc ctttctgacg ctgtcccgcg cggacgcgga atcccagcga
       61 tagagcccga actccctgat aatgtccttg caggcgggtg caatcttgat tttgccgctt
      121 tttatcgcct cggcggcgcg ccttatgccg tcaagcacat cgtttttagc ggggattaca
      181 ttgaacttgc ccttgcggcg aatgcactca ataaagctga cggcggaggg atcgactata
      241 accgcctcaa tatccctatc ccccgcgagc ttttcaaggt cggcgtagta ctcctcgtcg
      301 gttttctgat agccttccgc ccttgagtca aagtagaact cgtcgattct gtaccacaca
      361 ccccgatact cgccccagag cccaaaggat gcgggattca ctgtgccgta gtcgcaggag
      421 atgtaaaagc tgcggcactc cccctttggc aaagcgacgc atgcgctctc gctgaagaag
      481 ggatatacaa gcccctgagc cgccacccat ttgccctcca caaaccgctc gtaaaaggct
      541 ccgctgtaaa gggacttata cctttttata attgcgggtg aaagggaggg gttgtcctcc
      601 atggtaaagt ggagatacag gcagtttttc tcctttgcct tcaaaatcca ttcggtgtgg
      661 aaccaatgct gggggttttc cggattgcag ttgaaccaca tcttggagcc cttgacggag
      721 catcgggcta gagcctgctc cacaaaggag cggggcatca aggcaacctc gtccagcatt
      781 acgccgccca gggtcattcc ctgaatgaga gcggctgagg actcgtccct gcctccgaaa
      841 acatagaacc tgtttttgtt gccattaaac tcaatctcca caaggttttg ggagagtttc
      901 tcagtgcagg caaagcccag ctctttaaga acgggcagca aaggcacaag gacattgcgc
      961 cttaaagagg cgatggtttt gccgcagagg gcaaaggata tgccgctgaa gccggtcata
     1021 gcccagatta caaaggacag ccccatgcag attgtcttgc cgctccttac cgccccgtcg
     1081 cagataatgg catcaaagtc tttgtaaggg ctgccctcgc tccaccacga gagtacttta
     1141 agctgctttt cggaaaagct cttaaactcc aatcaaacca ccttttcgta aagttttgcc
     1201 ctgcccgccc cgccgcagag gggtgaaccg cccgtggggg cgcatcctgt cgggcggagt
     1261 gatctgcccc cacgaccgca accccgccgc cgtgggggtg tatcgtccgt acccgccgcc
     1321 gagagaatcc cgccgcagtg ggggtgtatc gtccgcgccc gtcgctgagg ggacttcgcc
     1381 atccgcttgt agggcaaacg ccgcacagcc cgcaggggcg ggagctgccg cagtcgtggg
     1441 cctcagcccg tctgggcggg tcggcaaaat ggttcggctt tggggcgaat gtcgggcagg
     1501 ctactcaccg ctaaagacgg cttttgcgct tttttcgatt gcctccacga aggggttttt
     1561 gtccgcctca cgctctgtct cggcaaggct ttgcagcttt tccagggcct tgagcctgtc
     1621 aataaacttt atctccatgc cgccgccttt gggacgctta atctcggaaa cgcagaaaag
     1681 gtccagcccg tcaagctcct cgggattaag attctcgtca aacataagcc taatggcgtc
     1741 cgctatgctg ccgaaagcgg cgcgtctgag tcccgccgag atgtccgccg tctttgcctt
     1801 tctcatctcg tcaagctggg aaatcatctc cctaatatcc tttcggctca tgagcttttc
     1861 cgccgcccgt tcaggaagta tcctgtagcc cgccttaaag gccgcctccc tggagtttct
     1921 cgttttcgag taatagtcgc agaaaagcct ctccttacgg cttagctcct tgcgcatcat
     1981 ttccctcccg taaaagattt tctcaaccat agctgaaaaa gcggcagaaa ttagcccaaa
     2041 caggcaaaca aaccgcccat tttgcgattt gtttacaaaa aatttacata tttcggaaat
     2101 ccagggcaga ataaatttgg cttattattt tttgttacag gcaaaaactt atagttttta
     2161 aagcagtttg cacaaaaatt ccgaataatt tcacattttg actttgcagt tttgtcagaa
     2221 atacttgcat ttgcttccta tatttggtac gcttacattg gaaaagttta gggggtgaac
     2281 ggtatgaaat atgcggactt gtctgaactg aatgaagccc ttaacagatt cgtctcaggc
     2341 gacgccgagg acgcttacga gtttatgggc tctcattttc tttccgatga caccgcggtt
     2401 ttcagagtct gggcacctaa tgccgagcgc gtgagcctct gcggcgactt caacgagtgg
     2461 aacaaagagg cccacccgct ggacccgatc ggattcggcg tttgggaaat catcctggag
     2521 ggcgtgaggc tttacgacaa ctacaaatac tgcatttaca cccgtgacgg caggcagatt
     2581 ttaaaaagcg acccctatgc cttccattcg gagacccgcc ccgccaccgc aagcaagctg
     2641 tataagctgg gcggctatac ttggacggac gacgagtggc ttaaaaagag aaaagccgag
     2701 cccatttact caaagcccgt aaacatttac gagcttcacg cgggctcatg gaaaaggcac
     2761 gaggacggca gcttctactc ctacagagag cttgccgaca gtctggtgcc atatattctc
     2821 gagatgggct acacccatgt ggagcttttg ccgctggctg agcaccccct tgacggctca
     2881 tggggctatc aggttacggg ctactattcc gtcacctccc gctacggcac ccccgaagac
     2941 tttatgtatt ttgtggacaa gtgccacaat gccggaatcg gcgttattct ggactgggtt
     3001 cccgcccatt tcccgcggga cgagcatgga ctaagagaat ttgacggcgg gccgctttac
     3061 gaatacgccg accccagaaa aggcgggcac ccccagtggg gcacctgcgt ttttgactac
     3121 ggcaggaacg aggtcaagag tttccttatc tccaacgctc tgttctggtt taaggagtat
     3181 cacatagacg gtttgagggt cgacgcggtc gcctccatgc tctaccttga ctactgcaga
     3241 ggagagggtc agtggactcc caatatctac ggcggcaacg agaatcttga ggccgtcgcc
     3301 ttcctgcaaa agctcaacac agcggttttc gctcatttcc ccgatgtgat gatgattgct
     3361 gaggagagca ccgcatggcc gttggtcaca aagcccgttc actgcggggg gctgggcttt
     3421 aactttaagt ggaacatggg ctggatgaac gacattctcc gctatttctc ccttgacccc
     3481 tatttcagaa aatacaatca cgaccttatc accttctcct tcttctacgc cttctctgag
     3541 aatttcattc tccccatctc ccatgacgag gttgtccacg gcaaaaagtc gctgcttgac
     3601 aaaatgcccg gcacctatga cgagaaattc gcctcggtaa gggcattcat gggctacgca
     3661 tacgcccacc cgggcaagaa actcattttc atgggtcagg agttcggaca ttttattgag
     3721 tgggatgaga aaaagcagct tgactggttc cttttaaact acgacaggca ccgtcagctc
     3781 aaggagtatg tcgccgctct caatcgtttc tacaaagaga atcctcccct ttggcagatt
     3841 gatttttcgt gggacggctt taagtggata gtcagcgacg acatagacaa ttccgtgctt
     3901 gtattcagac ggattaacga ggatgacaac gagattatcg ccgtcctcaa cttcactccc
     3961 gtaaaaaggg acatttaccg cttcggagtt cccgaaaagg gcgtttacga tattgttttc
     4021 aactccgacg aggagcgttt cggcggcacg ggctgcctta aaaagacctc tttcaaggcg
     4081 gaaaaatact ctatgcatga tttaaggtac agcatagaaa tcaccgttcc cccgctctcc
     4141 gccctgtttc ttaaaaagcg tgaaacaaaa aaagaaaata acaagtcaga aaaaacaagg
     4201 gcaaattaaa aaaagcggct tttgccgctt gtgaaaagga ggattaaaat gccgagaaaa
     4261 caagaatgca ttgccatgct gcttgcgggc ggtcagggca gcagactcgg aatccttaca
     4321 aaaaacatag caaaacctgc ggttccctac ggcggaaagt acagaattat tgactttccc
     4381 ctttcaaact gcgtaaattc gggcatatat acggtgggcg tactcaccca gtatcagccc
     4441 cttgagctta acgactatat aggcaacggt cagccctggg accttgacag ggctgagggc
     4501 ggagtgcaca ttctgccgcc ttatcagcag attaaaggct ctgagtggta caagggcaca
     4561 gctaacgcca tctatcagaa cattcacttt atagacaggt actcccccga ctatgtggcc
     4621 attctctcgg gcgaccatat ctataagatg gattatgaca agatgctgag ttaccacaag
     4681 gagaaaaacg ccgactgcac catagcctgc attgaggttc cgtgggagga ggcgtcccgc
     4741 ttcggtctta tgtttgtcga ctccgagggc agaatcaccg actttgagga aaagcctaaa
     4801 aaccccagga gcaacaaggc ctccatgggc gtttacattt ttacatggag caagctaagg
     4861 cagtatctta ttgacgacga ggctgacccc aattcctcca acgacttcgg caaggatgtc
     4921 atccccaaga tgcacagagc cggcgagagg atgtacgcct acgagttcga cggctactgg
     4981 aaggatgtcg gcaccatcga cagtctttgg gaggctaacc tagacctttt aaatccccaa
     5041 atcaacatcg accttgacga cgcctcatgg aagatatatt cccgcaacgc cgccgctccg
     5101 ccccactata tctccgccga ctcaaaggtg gaaaactccc ttgtttcagc cggctgcgag
     5161 attgagggcg agattgacta ttcggtgctt ttctccaatg tcaccgtaga aaagggcgcg
     5221 tgcgtaaaat actcaatcat catgcccggc gccgtcatta aggagggcgc tgtggttcag
     5281 tactccatag tcgcggagaa cgcggttatc gaaagcggcg ccgttgtggg cgaaaagccg
     5341 gagagcatga aagaccccag cgcatggggc gttgccgttg tgggcgcggg cgtgacaata
     5401 ggaaagggcg tcaccgtccc gtccaaagcg atgattgaca aagacatgaa ggggggaaac
     5461 taaaatgcgc gcaaggaata tcttaggcat aatctttgcc aatatgtacg atgactgcct
     5521 gcatgagctg acggctctca ggaccatggc gtcagtgccc ttcggcggca ggtatcgcct
     5581 tatagacttt cacctttcca acatggtcaa ctgcggcata gacaaggtgg gcgttgtcac
     5641 aaagggcaat taccgctcgc ttatggacca tctgggcaac ggcagaccct gggatttggc
     5701 aagaaaaagg gacgggcttt tccttctgcc gccctttaac gcggactcct cccgcagccg
     5761 cagctcaaga cttgaggctc ttattgacct tctcccgttt atagagagcg cggacgatga
     5821 gttcgtgata cttaccgact gcaatgtggt ctgctccttt gattacagcg ggctcatcga
     5881 cttccataag aatacgggag cggacatcac ggtctgctgc aagagaggaa tcagacccaa
     5941 tctcaacgac ctaatgacct ttgaaaccga ctctgactgc agagttacga aaatcgccgt
     6001 ttcccccaag ctgactgagg agtgcctgta ttccctcaac atcgtgcttt tgagaaagcc
     6061 ctttcttgag cggcttatag ccgaggcaaa gagccttaac taccgcagct ttacaaggga
     6121 cataatgcag agcaatgttg aaaaattcaa aatctacgca tatgagccgg aaggctttat
     6181 aaggactatc gactcgctta aaagctacta cgacatcagc atggagcttt taaagaggga
     6241 gaacagaaaa gagctgttct ccgcagacag acccgtattc acaaaggtta gggacgacat
     6301 gcccgcaatt tacggcataa actgcaaggc taaaaactcc ctcatagccg acggctgcca
     6361 cattgaggga gaggtggaaa actgcatact cttcagaggc gcaagggtgg gcaaaaacgc
     6421 cgttgtcaag aactctattc ttatgcaggg caccattgtc tcagagggcg ctcatcttaa
     6481 ctgcgtcata accgacaaga atgtgacggt cacccagggc aaaaacattt cgggcgccga
     6541 gagcttcccg atttatatat ctaaaggcat cagagtatag ctcagaaagg cgggattatt
     6601 ttgaaagtac tttatgcggt cagcgaggcc cgcccctttg ccgcaagcgg gggtcttgcg
     6661 gatgtagcag gctctctgcc ccaggctctt agaaaaaggc ttgtgggctg cagggtcgtt
     6721 atgccccttt acgccagcgt tcccgaggaa ctgaggcaga acatgaaatt catcacccac
     6781 atcagtgtac ccgtgtcttg gcggagccag tactgcggca tttttgaggc aaggcacggc
     6841 ggtgtaattt actatctcct tgacaatcag tattatttca agcgggacaa ctactacagt
     6901 tactatgacg acgcggagcg cttcgcgttc tttgcccgcg ccgtgctgga aatcataccc
     6961 cacataggtt ttgtgcccga cattatccac tgcaacgact ggcagacagc tttgacaccc
     7021 gtttatcaca gctgcttcta cgccaacaaa gagggctaca gccatataaa gaccattttc
     7081 accattcaca atatccagta tcagggcgtt ttcggcaggg agattctcca cgatgttctg
     7141 ggactcggcg atgagcatta ttcccttctc gaatatgacg g
//
LOCUS       DAONVQ010000100         7129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_7960_length_7129_cov_5.427198, whole genome shotgun sequence.
ACCESSION   DAONVQ010000100 DAONVQ010000000
VERSION     DAONVQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7960_length_7129_cov_5.427198"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(499..951)
                     /locus_tag="PLD98_06905"
     CDS             complement(499..951)
                     /locus_tag="PLD98_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06010.1"
                     /translation="MLYFEADAVLTLNGAQSAAHIRHIGFELTEMTFSAPANIKGLKM
                     TKTDDSVNFEFSGMKQGIKGSGPYNDSPALILSEILSAVQTGEGIETKRKTNEIKGKI
                     RNADFTVVRDADKNIQEITSEGLSFRAVFSNYKSLEAEILGTPSGITD"
     gene            complement(938..2617)
                     /locus_tag="PLD98_06910"
     CDS             complement(938..2617)
                     /locus_tag="PLD98_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQA06011.1"
                     /translation="MLVMNSEQIRKAEESAVLRGASFEEMMLKAGRNAAKEILKVFSG
                     RENALIVCGKGKNGGDGFVVADVFAAEFQKVTVLLPLGEPADELSRKMLSKIQGRENI
                     EIADLKKSDTESLEKAKAEIDKAQVIVDAVFGIGFSGAVKGDLAELINKINRSKAFVA
                     AIDIPSGLLSDCGELTETYVKADLTLAMYALKPVHVLKPAADECKAVRIIDIGIGEPE
                     ENKDEFVFKTASPFEVASRFKKRPHDCHKGTCGTVLIVAGSYEMPGAAVLASKAASIL
                     GAGLVITAFPDKAYNAITSKLTEQVLLPLPSNENGRISKTAEEKLLSALKKADALLIG
                     PGLGVDEDTEFIVKTLLSNTEIPTVLDADGINVISKNIDILKEVKAPLVLTPHPGEMS
                     RLCRTDICEITADKRLAALKFTQKFSPTLVLKGSNTVISKQGKAQYINATGCPALARG
                     GSGDLLCGMIAALMAGGMDEFESAVCAVYIHGLAGDYSARVFSETSAATELTLKCLPL
                     LLSRFLYKAGAELEKEDFGAVFNPFDYMRAVLMQERGWHGDKESLRRDALL"
     gene            2778..4205
                     /gene="pyk"
                     /locus_tag="PLD98_06915"
                     /pseudo
     CDS             2778..4205
                     /gene="pyk"
                     /locus_tag="PLD98_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
     gene            complement(4488..6431)
                     /gene="thrS"
                     /locus_tag="PLD98_06920"
     CDS             complement(4488..6431)
                     /gene="thrS"
                     /locus_tag="PLD98_06920"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQA06012.1"
                     /translation="MIKLTLKDGSIKEVEKGISAMDAVKEISMGLYKAACAVKINGEV
                     KDLRAVLQDDCSFEVLTFDDEEGKKAFRHTASHILAQAVKRLYPEAKLAIGPATDNGF
                     YYDIDMPVSITADDLPAIEAEMKKIVKENLPIERFELPADEALAMAEKEGEIYKAELI
                     REHSAKGEAISFYRQGEFADLCAGPHLPETGRVKAIKLTALTGAYWRGDAKNKMLSRI
                     YGTAFPKQSELDAYLAAVEEAKKRDHNKIGRELELFTTADIIGQGLPILLPKGARIIQ
                     ILQRFVEDEEQKRGWLLTKTPYMAKSNLYMLSGHWDHYRDGMFVIGDEEKDDEVFALR
                     PMTCPFQYQAYLSRARSYRDLPLRYNETSTLFRNESSGEMHGLIRLRQFTISEGHLMC
                     TPEQLEDEFRGCLELAIYMLKTLGLYEDVSYRFSQWDPNDREKYIGTKEQWDEAQGAM
                     EKILNHLEIPYKIGVGEAAFYGPKLDIQIKNVHGKEDTLITIQIDQMLAEKFGMEYTD
                     RDGTKKHPYIIHRTSIGCYERTLALLIEKYAGAFPVWLAPEQVRVIPIADRHFEAAEK
                     VLRELQSAGLRAELDTRSESLGYKLRDAQLQKIPYMLVIGDKEAESGTVSVRKRGVGD
                     IGAKPFAEFKELILKENEEKVIW"
     gene            complement(6763..>7129)
                     /locus_tag="PLD98_06925"
     CDS             complement(6763..>7129)
                     /locus_tag="PLD98_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06013.1"
                     /translation="LTLALYMADGQLERQIRRLRNIYSEKRRIFISELSRAFGAEGEI
                     HDNGSGLFVIFEVKTDKTKEEIKAAAEKNKIRLSFLSDFYMGNSPEKSGKRLLLYFSS
                     VKASDMEKAANLLKKTVLE"
BASE COUNT         1551 a         1784 c         1889 g         1905 t
ORIGIN      
        1 tgttcagggt caatcctgcg tggcggtggg ctaagtttcc tctcggcggc ggtgaaaccc
       61 ttggcggggc ggcgataacc tttgaggacg gcggcgggat tttttgagcg ttggcttatt
      121 tctgcggcgc ggcggggcgg tgtgatgggg gctgtcccct tctttcgggc gtggcagtgg
      181 tcttgggcgc gccttttgcg gctgcgctgc ggaattcggc tcggcggcgt tagtggcttt
      241 gcctgttcgg ggcgtccatg cgtaacggtt gtcccctcgt cggcggtgcg gcggttgagt
      301 gcgggttgcc cttagcacgg aagcgggcgg ctgccgcttc cctttttgcg cgggctgacc
      361 tcctcggcgt caaacggggg acggggttga gctttcactc ggcggggcgg tgtgttgtta
      421 ttccaaaata aaaaccgccc ggggaggggt ggtcgggcgg gttttgacac caaaaaagag
      481 aggggtcgcc tctctttttc agtctgttat gccggaggga gtacctagga tttcggcttc
      541 aaggcttttg taatttgaga aaaccgccct gaacgaaagt ccctcgcttg ttatctcttg
      601 aatattcttg tcagcgtctc tgactacggt aaagtcggcg tttctgattt tgccctttat
      661 ttcattggtt ttgcgctttg tttctattcc ctcgcccgtc tgcacggcgc tcaggatttc
      721 agagagaatc aaagcggggc tgtcgttata gggcccgctg cctttaatgc cctgcttcat
      781 ccccgaaaac tcaaagttta cggagtcgtc cgtctttgtc attttaagac cctttatgtt
      841 tgcgggggcg ctgaaggtca tctcggtcag ctcaaagcct atgtgcctta tatgggctgc
      901 gctttgcgct ccgtttaagg tgagaaccgc atcagcctca aagtagagca tctctccgga
      961 ggctttcttt atctccgtgc catccccttt cttgcatgag gacagcgcgc atataatcga
     1021 aaggattaaa aacagcgcca aaatcttctt tttcaagctc agcaccagct ttataaagaa
     1081 atcttgaaag aagtaaaggc aggcatttta aggtaagctc tgttgcggcg gatgtctcgg
     1141 agaatacgcg cgccgaataa tctcccgcca gaccgtggat ataaaccgcg cacacggctg
     1201 actcaaattc gtccatacct cctgccatta aagcggctat cattccgcaa agcagatcgc
     1261 ctgaaccccc tcttgcaaga gccggacagc cggtggcgtt tatatactgc gccttgcctt
     1321 gctttgaaat gacggtatta gagcctttca gaacgagcgt tggtgaaaac ttttgtgtga
     1381 atttcagtgc agcaagtctc ttgtcggcag taatctcgca gatgtcggtc cggcagagcc
     1441 ttgacatctc gcccggatga ggagttagga ccaatggcgc ctttacttct ttcaatatat
     1501 ctatattttt ggaaattaca tttattccat cggcgtcaag aaccgttgga atttctgtat
     1561 ttgaaagaag cgtttttact ataaactcgg tgtcctcatc cacgccgagt ccggggccga
     1621 ttagaagggc gtccgctttt ttgagcgctg agaggagttt ttcctccgca gttttgctaa
     1681 ttctgccgtt ttcgtttgag ggaaggggca aaagaacctg ctctgtgagc tttgaggtta
     1741 tggcgttata ggccttgtcg gggaaggcgg tgatgacaag accggcgccg agaatagaag
     1801 ccgcctttga agcgagaact gcggcgccgg gcatttcgta gctgccggcg acaataagaa
     1861 ccgttccgca ggtgcccttg tggcagtcat gggggcgttt tttaaagcgg gaggcgactt
     1921 caaagggcga ggcggtttta aatacaaatt cgtccttgtt ttcctcaggc tcgccgatgc
     1981 cgatatcaat gattcttacc gctttgcact cgtcggcggc gggtttaagc acatgaaccg
     2041 gctttaaggc gtacatggca agggttaaat ctgctttgac ataagtttcg gtaagctccc
     2101 cgcagtcgct taaaagacct gaggggatgt cgattgccgc cacaaaggct ttagagcggt
     2161 tgattttatt tataagctcc gccaaatcgc cttttacggc gccgctgaag cctatgccga
     2221 aaacagcgtc cacaatgacc tgagctttgt ctatctcagc tttggctttc tcaaggcttt
     2281 ccgtgtcgga tttctttaaa tctgctatct caatgttctc tctgccttgt attttggaga
     2341 gcatctttct tgaaagctcg tccgctggct ctccgagggg caaaagcacg gttacttttt
     2401 gaaactccgc ggcgaaaaca tcggcaacaa caaagccgtc gccgccgttc ttgccctttc
     2461 cgcagactat cagggcgttt tccctgccgc tgaaaacctt cagtatctcc tttgcggcgt
     2521 ttctgcctgc tttcagcatc atttcctcaa aggaggctcc ccttaatacc gcagactcct
     2581 cagcttttct tatctgctcg gaattcataa caagcatata aatccccctt caagctcagt
     2641 ttatcctttt ttatccaaaa tctcaagtaa ggagaagaaa atgttaattt gttcccatgc
     2701 cttgatttac taattaaaat gtatataata ttttgggata gaatttcact gaatttatat
     2761 ataaaggcgt tgtttatatg agaagaacca aaatagtatg cacaatgggt ccgtctacgg
     2821 agaatgagga tgttttaaga agaatgatgc tcagcggcat gaatgttgcc cgctttaact
     2881 tttcccacgc gaaccataag tggcacaaaa aaatgtttga tatggtggta aaggtcagag
     2941 aggagctgaa tctgccaata gccaccatgc ttgatacaaa gggacccgaa ataagggtcg
     3001 gcagatttaa aaacggcttt gctgagatta aaacgggcga cagctttgtt ttaagagccg
     3061 acaccgtttt gggcgatgaa acgggcgttt cgattgtagg tcacgacctt tcaagggatg
     3121 ttaaaaaggg caacacgata ctccttgacg acggtcttgt ggagctttat gtggagaaaa
     3181 tcgtcggcag ggacatacac tgcacggtca aaaacggcgg agctgtcaag gacaacaagg
     3241 gcgtgaatgt cccgaacgct gagcttacca tgccttacat cagcgagcgt gacagagcgg
     3301 acattatttt cgccatcgag aacggctttg actttatagc cgcgtcgttt gtccgctcag
     3361 cggcggatat tctagaaatc cgcaggattc ttcaggagca caactgcaac agaattcaca
     3421 taatcgcgaa gatagaaaac cgccagggcg tggacaacat agacgagatt atccgagccg
     3481 ccaacggcgt tatgatagcg aggggcgatt tgggcgtaga gctccccctt gaggaaatcc
     3541 cggttattca gaagatgatt ataaagaaag cctacaacgc gggcaagcag gtcattacgg
     3601 cgactcagat gctggagtct atggtcaacc accccagacc cacaagagct gaggctaccg
     3661 atgtggcgaa cgccatttac gacggcacca gcgccgtaat gctctcgggc gagaccgccg
     3721 ctggcaaata ccccgttgaa gcggtcgcaa ccatggcgag gattgcggaa aaagcggaga
     3781 gcgacataaa ctactccaag cgttttaagg agcgggaggt tgacatcgcc tccgaaaaca
     3841 taaccgaggc tatcgccaac gccatctccc acgccacctg cacaacggcg acggatttgg
     3901 gcgccgcggc gataattacg gttacaaagt cgggcaagac cgcaaggatg atttcaaaat
     3961 tccgcccggg cagcccgatt ataggctgca gcacctatca tcacatttgc aggcagctga
     4021 atctctcgtg gggcgtgact cccctgctgc tggaagaaaa gaaaaccagc gacgagctct
     4081 ttgaccatgc ggttgaagtg gcgaaaaaag cgggacttgt ggttgagggc gaggttgtgg
     4141 cgattaccgc aggcgtgccc gttggcgtcc ccggcaatac gaacatgatt aaggttacgg
     4201 ttgtttaata aaaagccctg aagattgctt cagggcttta gttttgccgc caaagggcgg
     4261 ttttttgttt ttagaggcac ttgcggcggg aggcgggtat agcctctgcg ggcggcgaac
     4321 atccttcttg cggcgggacc ggggcgggct tatcacccga gcgggcggag ggacaggcgg
     4381 cgggggcgga gcggctgaaa aaactgctcc cgttcgggca gggctgactt gcgcaagttt
     4441 ccccaaccct acgcaaaaaa tgaaagcccc aaaatggggc tttcggttta ccatataact
     4501 ttttcttcgt tttctttaag gataagctcc ttgaactcag cgaagggttt tgcgccgatg
     4561 tcgccaacgc ccctcttgcg gacagaaact gtgccgctct cagcttcctt gtcgcctatt
     4621 acaagcatat agggaatctt ctggagctgg gcgtctctga gcttgtagcc aaggctctcg
     4681 cttcttgtgt caagctcagc tctcagacct gcgctctgca gctctctgag caccttctcg
     4741 gccgcctcaa agtggcggtc ggcaatcggg atgaccctga cctgctcggg ggcaagccat
     4801 acggggaacg cgcctgcgta cttctcaatc agcagagcca gtgtgcgctc atagcagccg
     4861 atggatgttc tgtgaatgat gtagggatgc ttctttgtgc cgtccctgtc cgtatattcc
     4921 atgccgaact tttcggcaag catctggtca atctggattg tgatgagcgt atcctctttg
     4981 ccgtggacat tcttaatctg aatgtcgagc ttcggtccgt agaatgcggc ctcgcctacg
     5041 ccgattttgt aggggatttc gaggtggttg agaatcttct ccatggctcc ctgagcctca
     5101 tcccactgct cctttgtgcc gatgtacttc tctctgtcgt tggggtccca ctgggagaag
     5161 cgataggata catcctcgta aagtcccaag gttttcagca tatagattgc caactcaagg
     5221 cagcctctga actcgtcctc aagctgctcg ggagtgcaca taaggtgacc ttcggagatt
     5281 gtgaactgcc tgagccttat aaggccgtgc atttcgccgc tggactcgtt tctgaaaagg
     5341 gttgatgttt cgttgtatct gaggggcaaa tccctgtaag agcgggctct gctgagatat
     5401 gcctgatact ggaaggggca tgtcatgggg cgcagggcaa agacctcgtc gtccttctcc
     5461 tcgtcgccga ttacaaacat accgtccctg tagtggtccc agtgacccga cagcatatag
     5521 aggttgctct ttgccatgta gggagtcttt gtgagcagcc atcccctctt ttgctcctcg
     5581 tcctcaacaa agcgctggag tatctggatt attcttgcgc ctttgggcag gagtatgggc
     5641 agaccctgac cgataatgtc ggctgttgtg aagagctcca gctctctgcc tatcttgttg
     5701 tggtcccttt tctttgcctc ctcaacggcg gcaaggtaag cgtcaagctc ggactgcttg
     5761 gggaatgccg tgccgtaaat gcggctgagc atcttgtttt tcgcgtcgcc tctccagtaa
     5821 gcgcctgtga gggctgtaag ttttattgcc tttaccctgc ctgtttcggg cagatggggt
     5881 cccgcgcaaa ggtcagcgaa ctctccttga cgatagaagg agatggcctc gccctttgcg
     5941 gagtgctctc ttataagctc cgccttatag atttcgcctt ctttttctgc catggcgagt
     6001 gcctcgtccg cgggcaattc aaagcgttct atgggcaggt tctctttgac aatctttttc
     6061 atctccgcct cgattgcggg caggtcgtct gccgtgatgc tgacgggcat gtctatgtca
     6121 taataaaaac cgttgtcggt tgcgggaccg atggcgagct tagcctcggg ataaaggcgc
     6181 tttacggcct gggcgaggat gtgggaggca gtgtggcgga atgccttttt gccctcctcg
     6241 tcgtcgaatg tcaggacttc aaagctgcag tcgtcttgga gcacagcgcg caaatcctta
     6301 acttcgccgt taatttttac ggcgcaggct gccttgtaaa gtcccatgct tatttctttt
     6361 acggcatcca tggcgctgat gcctttttca acttctttta tcgagccgtc tttcaatgta
     6421 agtttaatca taatctatat cctctctgtg cagttattag aatcaaaaaa ctccaccccg
     6481 aaactggggt gaagcaaagc tccacggttc cacccgtatt gcagcataag ctgccactca
     6541 ttctgccttt aacggggcaa gcgtcgacct taatccaaac ggttttcggc cgctctcggg
     6601 agtggtaact ttttcggggc agtatatcca ttccaccaaa caggatactc tctgaaaagc
     6661 cctggtgaaa aagccttctc cgtcaacgat ttgacatatt tacattttca ttatattacc
     6721 catgtcggga tatgtcaata tccgcaatat tcctctgttt tgctattcca gaacggtctt
     6781 tttcagaaga tttgccgctt tttccatatc ggacgctttg acgctggaaa agtataaaag
     6841 aagccttttg ccgctttttt cggggctgtt tcccatataa aagtcgctga ggaagctcag
     6901 gcggattttg tttttctcgg cggctgcctt tatttcctct tttgttttgt ccgtttttac
     6961 ctcaaatatt acaaagagtc ccgagccgtt gtcgtgtatt tcaccctctg ccccaaaagc
     7021 gcggctcagc tccgaaatga agattcttcg cttttcgctg tagatgtttc tgagccttct
     7081 tatctgccgc tcaagctgac cgtcagccat atagagggcg agagtaagc
//
LOCUS       DAONVQ010000101         6743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_8510_length_6743_cov_4.532147, whole genome shotgun sequence.
ACCESSION   DAONVQ010000101 DAONVQ010000000
VERSION     DAONVQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8510_length_6743_cov_4.532147"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PLD98_06930"
     CDS             complement(<1..576)
                     /locus_tag="PLD98_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HQA06014.1"
                     /translation="MALTTNKTVLSWIEEQIELVKPDKVVWIDGSKEQIEALRAEACA
                     TGEIIKLNEELLPECFLHRTAPNDVARVEHRTFICTPTKEEAGPTNNWKDPEECYAML
                     REIAKDSYKGRTMYVIPYSMGPIGSPLSKIGIELTDSIYVVLNMAIMTRIGKAVLDVL
                     GDSNDWVRGLHCKCDIDEEKRYICHFPQDNTI"
     gene            complement(709..1716)
                     /gene="tsaD"
                     /locus_tag="PLD98_06935"
     CDS             complement(709..1716)
                     /gene="tsaD"
                     /locus_tag="PLD98_06935"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQA06015.1"
                     /translation="MRILAIETSCDETAAAVVEDGRKVLSSIVASQIDEHIKYGGVVP
                     EIASRMHSESITAIVREALNSAGLKLTDIDAVAVTFAPGLIGALLVGVNYAKGLAYSA
                     GLPLIPVHHIRAHIAANYISSPELEPPFLALVVSGGHSHLVEVRDYTEFKVLGRTRDD
                     AAGETLDKTARVMGFPYPGGANMDRACKDGNPHAFNLPRPHVEGSPLDFSFSGLKTAV
                     LNLINSANQKGEEINFKDLGASVMERVTGCLVENTLEAAKLTRLTKIVAAGGVAANSV
                     LRAKMRDMCEKNNFELFIPPLSLCGDNAAMIGAQGYYEFLAGVKADLNLNAFASKDID
                     F"
     gene            complement(1731..2591)
                     /gene="rimI"
                     /locus_tag="PLD98_06940"
     CDS             complement(1731..2591)
                     /gene="rimI"
                     /locus_tag="PLD98_06940"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HQA06016.1"
                     /translation="MKHILFVCTGNTCRSPMAEGIFNVFIKRFKFNNLSCSSAGTAAF
                     TGSEASEYAVEAAKAFGADISHHRSRALTPYLIDSADLIVCMTSAHLNVLLPYAPREK
                     LRVLGDGIPDPYGGSQRDYLRSAYKIARGLIELIDIDLALEIVPFKERHIPEAVRLEN
                     ISFSVPWSENAFRSELENPKARFFAAEYAGSVIGYIGAHNILSEVFITNISVLPSFRR
                     RGVASKLLKHIIDISVSENADLITLEVRESNMAAIELYQKFGFQKAGVRKAYYEKPTE
                     DAIIMTKYLK"
     gene            complement(2604..5219)
                     /gene="polA"
                     /locus_tag="PLD98_06945"
     CDS             complement(2604..5219)
                     /gene="polA"
                     /locus_tag="PLD98_06945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQA06017.1"
                     /translation="MRLLLIDGNSIINRAFYGIKLLTTKDGKYTNGIFGFLNILLSLL
                     DSIKPDAVVAAFDLKAPTFRHDMYEGYKAHRKGMPPELAEQLPVLKDIIRAFGYKVVE
                     KEGFEADDIIGTLAYNSGAECYVATGDRDSLQLVSDKVKVVLATTKAGSPVSTIYDEA
                     KVFEEYSVTPEKLIDIKALMGDASDNIPGVAGIGQKTACDLVAKYGSIEEIYSKIDEL
                     EISPSVKAKLINGKDSAFLSYKLGTIEKNVPISCDINDYLPEKRNDERLAGILTSLEM
                     YKMLDRLGLKAVSLPEPEKIAEPLESLRLEAEFDSEELINKVKSQKALYLNLDSNTVT
                     ISRYCVNCGGVLYFKRIEDEGAEDLLIKLLSDADTEKHFNFSKAAHYFALQRGVSIKN
                     VKSDAQLAGYLINPNVSDYSVNTIAAEHSIPIPEIVVSENQQEIYNANLGLIESAALL
                     PAVCQATGKILSKLGQAELYTDIELPLSKVLASMELVGFEVDKSGIELFGIDISNKLN
                     DITQRIYSFAGEEFNINSPKQLGEVLFEKLRLPVKKKTKTGYSTNAEVLESLRFEHPI
                     IPEILEYRTLAKLKSTYCDSLVKEIREDGRIHSTLNQTETRTGRISSTDPNLQNIPVK
                     QDIGRELRRFFKAKEGFVLVDADYSQIELRVLAHMADDKNMIEAFNEGQDIHAITASQ
                     VFKMPLDMVTPLMRSRAKAVNFGIVYGIGAFSLSQDIGVSIQEADRYIKAYLSHYSGV
                     ANFMKRAVETAKEQGFASTIFNRRRYLPELKSSNANLRAFGERVAMNMPIQGTAADII
                     KLAMIKVYDRFEKENMKSRLIMQVHDELIVESPEEEKEKAAEILKTEMENAVKLKVKL
                     IADVNTGKTWYDAKG"
     gene            complement(5340..>6743)
                     /locus_tag="PLD98_06950"
     CDS             complement(5340..>6743)
                     /locus_tag="PLD98_06950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQA06018.1"
                     /translation="SGMGKLYTGFDANLMPADQTTKNDEDQEITFEQLFADYVKDHVK
                     QYKTVCDKARQEGFEISEEGKAEVDEIIESYRKQAQSVQGGEYSLNRFLSVVIISGMN
                     EKTLRKILTEQTLYEEYMTAKQDEFRKGITEAEIEKEYKDNRTEYDKVSLRLFGFEIE
                     DEDDKKDDKKDEDKKDDKKEEEKAPTKAELKAKEFLSKIKDEASFLKLAEYYYDGEDK
                     DEYNSDDNTKVVNVDYDTVKANVSEEAAKWLFSTDRKVGDKAAYASSDYWYIMYITSP
                     AGRDEVKPVNVRHILIKFEDTDKDGKKIELTDEKKAEYKKKAEDIYNEFLNGDKTEQS
                     FAALAEKYSDDKASLAAAGSTEGGLISNMERGQYVKQFENWAFDPSRKPGDTEIIETT
                     YGYHIMYFVSTNEEPAWRTTAKDTISSEKTQKFFDDMMENSPFEIVAEDKAVKRALKR
                     INKKIAEGISASARTSA"
BASE COUNT         1542 a         1701 c         1548 g         1952 t
ORIGIN      
        1 gattgtattg tcctgcggga agtggcagat gtacctcttc tcctcgtcaa tatcgcactt
       61 gcagtgcaga cctcttaccc agtcgttgct gtcgcccaga acatccagaa cagccttgcc
      121 tattctggtc ataatggcca tgttgagaac aacatatatt gaatccgtaa gctcaatgcc
      181 tatctttgaa aggggagagc cgataggacc cattgaatag gggatgacat acatagttcg
      241 gcctttgtag gagtccttgg ctatctcacg aagcattgca tagcattctt cggggtcttt
      301 ccagttgtta gtgggacctg cctcctcctt ggtgggggtg cagatgaaag ttcgatgctc
      361 aacacgagca acatcgttcg gagcggttct gtgaaggaag cactcgggaa gcagctcctc
      421 gttcagctta ataatttctc ccgttgcgca ggcctcagcc ctaagagcct caatttgctc
      481 tttgctgccg tctatccaaa ctaccttgtc gggcttgaca agctcgatct gctcctcaat
      541 ccaggatagc acggttttgt tggttgttaa tgccatcaga agcaatctcc tttgctataa
      601 aatatacaga aaaccttaag tcccttgcag cgtctttgct gtttcggggg ccttaaggat
      661 aatcatcaaa aaaacaacaa aatcaaacct aaaataacat attctttttt aaaagtcaat
      721 atctttgctg gcaaaagcgt ttaaatttaa gtcggctttc acgcccgcca aaaactcata
      781 gtagccctgt gcgccaatca tagcagcgtt gtcgccgcag agggaaaggg gaggaataaa
      841 aagctcgaaa ttatttttct cgcacatatc tctcattttg gctctcagaa cggagtttgc
      901 ggcaactccg cccgctgcaa cgattttggt aagacgagtc agctttgccg cctcaagggt
      961 attctcaaca aggcagcccg tgaccctttc cataacggac gctcccaaat ccttaaagtt
     1021 tatctcctcg cccttttgat ttgcgctgtt tatgagattg agcacagcgg ttttaagacc
     1081 cgaaaagctg aaatcaaggg gactgccctc cacatggggg cgggggagat taaaggcatg
     1141 gggattgccg tctttgcatg ccctgtccat attggctccg ccggggtagg gaaagcccat
     1201 aaccctcgcg gttttgtcaa gtgtctcacc ggcggcgtca tcccttgtcc tgcccagaac
     1261 cttaaattct gtatagtcgc gaacctccac aagatggctg tgcccgcccg aaaccacaag
     1321 agcaaggaaa ggcggctcca gctcaggtga gcttatataa ttcgccgcaa tatgagctct
     1381 gatatgatgc acgggtatta aaggcagacc cgcggagtac gcaaggcctt tagcgtagtt
     1441 tacccccaca agcaaagcgc cgataagacc cggggcaaag gttactgcaa cggcgtcaat
     1501 gtcagtgagc tttagccctg cggagtttag agcctccctg acaatagcgg ttatgctttc
     1561 ggagtgcatt cttgaggcga tttcgggcac aactccgccg tactttatat gctcgtcaat
     1621 ctgagaagca acaatagagg acagcacctt tctgccatcc tcaaccactg ccgctgcggt
     1681 ttcatcgcat gatgtttcta tcgcaagtat tctcatatat ttcctttctc ttatttcaag
     1741 tactttgtca tgattatagc gtcctcggtg ggcttttcgt aataagcctt tctgactccc
     1801 gccttttgaa agccgaattt ctgataaagc tctatcgccg ccatgtttga ctctcggact
     1861 tccagcgtta taaggtcagc attctctgaa accgaaatat cgattatgtg tttaaggagc
     1921 tttgacgcca cgccccttct tctgaaggag gggagaacag aaatatttgt aataaaaacc
     1981 tcggacaata tgttgtgagc gccgatatag ccaatgacgg agccagcgta ctcagcggcg
     2041 aaaaatcttg ccttgggatt ctccagctcc gacctaaagg cgttttccga ccatggaacg
     2101 gaaaagctga tattctcgag ccttaccgcc tccgggatat gcctttcctt aaagggtacg
     2161 atttcaaggg caaggtcaat gtctatcagc tcaataagac cccttgcgat cttgtatgcc
     2221 gaccttaaat agtccctttg actgccgccg taggggtcgg ggattccgtc acccaacacc
     2281 ctgagctttt ccctcggcgc atagggcagc agcacattca aatgagcgct tgtcatgcag
     2341 acaataaggt cagcgctgtc tataaggtag ggcgtcaatg cccttgagcg gtgatgggaa
     2401 atatccgcgc cgaatgcctt tgcggcttcg acagcatatt ccgacgcctc gctgcccgta
     2461 aatgcggctg tgcctgcgct cgagcagctc agattattga atttaaacct cttaataaac
     2521 acattgaata tgccctccgc catggggctg cggcaggtgt tgccggtgca aacaaaaagt
     2581 atgtgcttca ttgaatccct cacttaaccc tttgcgtcat accatgtctt gccggtgttt
     2641 acatcggcaa taagttttac cttgagtttt accgcgtttt ccatctcggt tttaagaatc
     2701 tcagcggctt tttccttctc ctcttcggga gattccacaa tcagctcgtc atgcacctgc
     2761 atgataagcc ttgatttcat gttctctttc tcgaatctat cgtaaacctt aatcatggca
     2821 agctttataa tatccgccgc ggtgccctga atgggcatgt tcatggcgac cctttcgcca
     2881 aaggctctga ggttcgcgtt ggaggattta agctcgggca gataccttct tctgttaaag
     2941 attgtagagg caaagccctg ctccttagcc gtttccacag cccttttcat aaagttcgcg
     3001 acgcccgagt aatggctgag atacgccttt atatacctgt ccgcctcctg aatgctgacc
     3061 ccgatgtcct gagagaggga gaatgcgccg atgccgtaaa ctatgccgaa gttgaccgcc
     3121 ttagccctgc ttctcataag gggagtgacc atatcaaggg gcatcttaaa gacctgagat
     3181 gcggtaattg cgtgaatatc ctgaccctca ttgaacgcct caatcatatt cttgtcatcc
     3241 gccatgtggg caaggactct cagctcaatc tgagagtagt cggcgtcaac cagaacaaag
     3301 ccttccttag ccttaaagaa tcttctcagc tccctgccga tgtcctgctt tacggggatg
     3361 ttctggagat tggggtccgt ggaggaaatc ctgcccgttc ttgtctcggt ttgattgagc
     3421 gtggagtgaa tcctgccgtc ctcgcggatt tctttaacaa ggctgtcgca gtaggtggat
     3481 ttgagctttg cgagggtcct gtattcgagt atttcaggga ttatcgggtg ctcaaatcta
     3541 aggctctcaa gcacctccgc attggtggag tagcccgttt ttgtcttctt ctttacgggc
     3601 agacgcagct tttcaaacag cacctcgccg agctgtttgg gcgagttaat attaaactcc
     3661 tcgcccgcaa atgagtagat tctctgcgta atatcgttca gcttgtttga aatgtcgatg
     3721 ccaaacagtt caatgccgct tttgtccacc tcaaagccca caagctccat ggaagcgagt
     3781 acctttgaaa ggggcagctc tatgtccgta taaagctccg cttgtccgag cttagacaaa
     3841 atctttcccg tcgcctggca tacagcgggc agcagtgcgg ctgactcaat aaggccgaga
     3901 tttgcgttat agatttcctg ctgattttcg ctgacaacta tttcgggtat gggtatgctg
     3961 tgctcagcgg caattgtgtt gacggaataa tccgatacat tcgggtttat aagatagccc
     4021 gcaagctgag cgtcggactt aacattttta atgctgacgc ctctttgaag ggcgaaataa
     4081 tgagcagcct ttgagaaatt aaaatgcttt tcggtgtccg cgtcggatag aagttttata
     4141 agcaaatcct ccgcgccttc atcctcaatt ctcttaaagt ataaaactcc gccgcagttt
     4201 acgcagtatc tgctgattgt taccgtattg ctgtcaagat taagatacaa agccttttgg
     4261 ctttttacct tgtttataag ctcctctgag tcaaactctg cttcaagacg caggctctcc
     4321 aaaggctccg caatcttttc gggctcgggg agcgatacag cctttaagcc cagcctgtcc
     4381 agcattttat acatctcaag ggatgtcaaa atacccgcaa gcctttcatc attacgcttt
     4441 tcgggcagat aatcgttaat gtcgcaggag atgggcacat ttttttcaat ggtaccaagc
     4501 ttatagctta aaaacgccga atccttgccg tttataagct ttgcttttac agaaggggaa
     4561 atctcaagct cgtctatttt tgagtaaatc tcctcaatgg agccgtattt cgccacaagg
     4621 tcgcatgcgg ttttttgacc gatacccgcg acgccgggga tattgtctga cgcgtcgccc
     4681 attagagcct ttatgtcaat gagcttttcg ggagtgaccg aatactcctc aaaaacttta
     4741 gcctcgtcat aaattgtgga aacggggctg cccgcctttg ttgtggcgag aaccaccttt
     4801 accttgtcgc ttacaagctg cagggaatcc ctgtcgcccg tcgccacata acactccgcc
     4861 ccgctgttgt aggcaagagt gcctattatg tcgtcagctt caaagccttc cttttcaaca
     4921 accttgtatc cgaaggctct tattatatct ttcagcacgg gcagctgctc ggcaagctcg
     4981 ggaggcatgc cctttctgtg agccttgtag ccctcgtaca tatcatgcct gaaagtaggt
     5041 gcctttaaat caaatgcggc tacaacggca tcgggcttga ttgaatcgag cagcgacaaa
     5101 aggatattaa gaaagccgaa aatgccgttt gtgtattttc cgtcctttgt ggtaaggagt
     5161 tttattccgt aaaacgcacg gtttataata ctgtttccgt ctatcagcag cagcctaata
     5221 tcaatcaccg ctttcgtatt tgcaaataaa tccaaaaata gtttatcaca aaagagcctt
     5281 aaataaaagt aaaatcgcac aaaaaaagac ggatgcgtaa gcacccgtct gtaatttcgt
     5341 tatgctgaag ttctagcgga ggcggagatt ccttcggcta ttttcttgtt gattctcttc
     5401 agtgccctct ttactgcctt atcttcagcc acaatctcaa atggactgtt ctccatcata
     5461 tcatcaaaga atttctgagt cttctctgat gagatggtat ccttggcggt ggttctccaa
     5521 gcgggctcct cgtttgtgga aacgaagtac atgatgtgat agccgtaggt ggtttcgatt
     5581 atctcggtat ctccgggctt tctggagggg tcaaatgccc agttctcgaa ctgcttgaca
     5641 tattgtccgc gctccatgtt ggagataagt ccgccttcgg ttgagcctgc tgcggcgagg
     5701 gaagccttgt cgtcgctgta cttttcggca agagccgcga aagactgctc agtcttgtcg
     5761 ccgtttaaga attcgttgta gatgtcctct gccttcttct tgtactcggc cttcttctcg
     5821 tcagtcagct caattttctt gccgtccttg tctgtgtctt cgaacttaat aagaatgtgg
     5881 cgtacattta cgggctttac ctcatccctg cctgcggggg atgtgatata cataatgtac
     5941 cagtagtcgg aggatgcgta tgcagccttg tcgccaacct tgcggtcggt ggagaagagc
     6001 cattttgctg cctcttcgct tacattggcc ttgactgtgt cgtagtcaac attgacaacc
     6061 tttgtattgt cgtcgctgtt gtactcgtcc ttgtcctcgc cgtcatagta gtactcggca
     6121 agctttaaga aggaagcctc atccttaatc tttgaaagga actcttttgc tttcagctct
     6181 gcctttgtgg gagccttttc ttcttccttc ttgtcgtcct tcttatcttc gtctttcttg
     6241 tcgtctttct tatcgtcctc gtcttctatt tcaaagccga agagtcttaa agaaaccttg
     6301 tcgtattcgg tgcggttgtc cttgtactct ttttcgattt cagcctcggt aatgcccttt
     6361 ctgaactcat cctgctttgc ggtcatgtac tcttcataga gagtctgctc tgtcaggatt
     6421 tttctgaggg tcttctcgtt catgccgctg atgattacaa cggagaggaa tctgttaaga
     6481 gagtactcgc cgccctgaac gctttgggcc tgctttctgt agctctctat aatctcgtca
     6541 acctcagcct tgccttcttc gctgatttca aagccttcct gtcttgcctt gtcgcataca
     6601 gtcttgtact gctttacatg gtccttgaca taatcggcaa aaagctgctc aaaggtaatt
     6661 tcttggtcct catcgttctt agttgtctgg tcagcgggca ttaagttagc gtcaaagcct
     6721 gtatagagct tgcccatgcc gct
//
LOCUS       DAONVQ010000102         6686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_8611_length_6686_cov_4.790831, whole genome shotgun sequence.
ACCESSION   DAONVQ010000102 DAONVQ010000000
VERSION     DAONVQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8611_length_6686_cov_4.790831"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="PLD98_06955"
     CDS             complement(<1..1608)
                     /locus_tag="PLD98_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA06019.1"
                     /translation="MKTVIIGGVAGGASAAARLRRLDESAEIILLEKGPYISFANCGL
                     PYYLGGEIKEREELLLQTPESFYNRFKIDVRVLSEAVKIDRQRKVVHIKNLKDNTEYE
                     ESYDKLILSPGAKPIIPNIKGINRKNVFTLRNIPDTDKITEYIAEYSPRSAAVIGGGY
                     IGLEMAENLYLRGLEVTVLEKASHVIGSLDFDTAVEVHNYIRSKGINLILGEAAEEIT
                     DSPDGKTIIKTANREIKTDMIILSIGVTPDTALAEDCGLELAPNNAVITNEHMQTSDK
                     DIYAVGDCVMVKNLVTGEPAHIPLAGPANKQGRIAADNICGIKSVYKGSIGSAILKFF
                     DLTVAACGINERAAKASGLDYDKVFLYALSHAGYYPGARNISMKVLFENKSGRILGAQ
                     LVGFKGVDKRCDVLSAAIRHGFTAYDLEELELCYAPPFSSAKDPVNMAGFVITNIIEG
                     IVKQYHWHDIDRLSKDENVTLLDVRTEREAARGCLERSINIPLDSLRDRLNELDKSKP
                     VYVVCQSGLRSYIACRLLSQNGFDCYNL"
     gene            complement(1623..1949)
                     /locus_tag="PLD98_06960"
     CDS             complement(1623..1949)
                     /locus_tag="PLD98_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQA06020.1"
                     /translation="MFGRYSARFNNITMAQAREALERDPSIILIDVRSREEYASGHIM
                     GSINIPVDEADAIVDIIDDKDAKIFVYSKSGARSRRACAEFSRKGYTDVTNIGGIRSW
                     TGFTVR"
     gene            2736..3728
                     /locus_tag="PLD98_06965"
     CDS             2736..3728
                     /locus_tag="PLD98_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA06021.1"
                     /translation="MKVYKSAVIGCGNIHRNHIKGINDSPLGKLTAVCDIDQEAADKS
                     ASENGVPAFYSLDELLKFGDFDVLHICTPHYLHAPMAIKAMRQGKHIICEKPMAIHSA
                     DAREMIKVAEETGVQLGVCFQNRYNSSSIYVKELLESSRMGKILGARGIVTWDRSEAY
                     YASGEWRGKIATEGGGVLINQAIHTLDLLQWFVDSPVKELKASISTKRLQGVVETEDT
                     ADALIFFENGVRGVFFASLCYTSNSPVMLELECENGSILLADKLTVKINGEEDIVLDI
                     NRPSCEKDYWGSGHTCLIHDFYKSLSENKPFAIDGEEGIKAVEIVEAIYDYARK"
     gene            3999..5222
                     /locus_tag="PLD98_06970"
     CDS             3999..5222
                     /locus_tag="PLD98_06970"
                     /inference="COORDINATES: protein motif:HMM:NF020571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1887 family CARF protein"
                     /protein_id="HQA06022.1"
                     /translation="MTLVELFDKNPVENIISSLVLQPQRVIFVGRKEIMAGHKRIIEK
                     LFNERRLKTDIHFLHPDRNSLDCAVELLSEIQKKFPDCEFDLTGGDDLMLLAMGVLME
                     KSPTKPKVHRYFISDRSIVGIDIDGSETRKTPFYLSVGETINLYGGSVLKSFKYESRW
                     DMPEDFKDDTDKMWGLCRKDTGLWNRLTGVLADFEHMKEKRDSLETVLSLSEIRSHLS
                     NKNQSFDAVEKFLQSLCSLKLIQDTGSDNAFLRYRFKNELIRHCLVKAGTVLELKILM
                     IARGIRDEKDEPFFNDALQGVNIDWDGKRHSSPFIAETENEVDVILMRGHIPIFISCK
                     NGFTDDEELYKLNTVAEKFGGPYVKKVLIATKLTGRNRSLEYFRQRAKDMGITLIENA
                     HLMNDEDLAEKIKGL"
     gene            complement(5241..5795)
                     /locus_tag="PLD98_06975"
     CDS             complement(5241..5795)
                     /locus_tag="PLD98_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQA06023.1"
                     /translation="MKVFVLFSSPNKNGYTKKLLDGFFVSAPENLSVDTVNLFDLLPK
                     PCNDCKACGKENTCVFDDLDEVFDKMQNADVVIIATPVYHYNVPAPLKAVFDRTQRFY
                     EAYVVRKEEPKRKNDGRGVLLTVSGRKAKQPVELIKKQTDRAFALLGINLAGSVHMGE
                     TDKGISDSEAIRQAEELGKKIFSK"
     gene            complement(5858..6280)
                     /locus_tag="PLD98_06980"
     CDS             complement(5858..6280)
                     /locus_tag="PLD98_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016324325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06024.1"
                     /translation="MNLTEKAAYLKGLAEGLGLDPENKETKVINAIIDLLDDLALTVA
                     DNEDEIALITEQLDAVDEDLDALESYVYDDEECDCDICDDFDEDDLYELECPSCKEIV
                     CFDSSIFDGDEQLTCPNCGEVFDKFEIVNEDDEEKNEE"
     gene            complement(6409..>6686)
                     /locus_tag="PLD98_06985"
     CDS             complement(6409..>6686)
                     /locus_tag="PLD98_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQA06025.1"
                     /translation="GDKLDDNFKYVKEDMVCKVLSYKGNVFGVEPPNFVELRVAKTEP
                     GFKGDTATNTLKPAVLETGAEIKVPLFIEEGELIQVDTRTGEYMSRA"
BASE COUNT         1786 a         1508 c         1486 g         1906 t
ORIGIN      
        1 cagattgtag cagtcaaaac cgttttgaga aagcagcctg caagctatgt agcttctgag
       61 cccgctctgg caaaccacat acacgggctt tgacttgtcc agctcattca gcctgtctct
      121 gagactgtca aggggaatgt tgatactgcg ctcaaggcag cctctcgccg cttctctctc
      181 agttctgaca tcaagtaggg ttacattttc gtcctttgac agcctgtcga tgtcgtgcca
      241 atggtactgc tttactatac cctcaataat gtttgtaatc acaaagcccg ccatgttgac
      301 agggtccttt gcggatgaga agggcggtgc gtagcaaagc tcaagttcct caaggtcata
      361 ggcggtaaag ccgtgcctta tcgccgcgga gaggacatcg cagcgcttgt ccactccttt
      421 aaagccgaca agctgagcgc ccaaaatcct gccgcttttg ttttcaaaca aaaccttcat
      481 gctgatattc cttgccccgg gataataacc cgcatgtgat aaagcatata ggaaaacctt
      541 gtcatagtca agccctgacg ccttcgcggc gcgctcattt attccgcagg cagcgaccgt
      601 caggtcaaag aatttcagaa ttgccgagcc tatgctgccc ttgtaaacgc ttttaattcc
      661 gcagatattg tccgcggcga ttctgccctg cttgttggcg ggtcccgcaa gggggatatg
      721 ggcgggctca cctgtcacaa ggttttttac cattacgcag tcgccgacgg catagatgtc
      781 cttgtcggag gtctgcatat gctcgtttgt gattaccgca ttgttcgggg caagctccag
      841 tccgcaatct tcagccagag cggtgtcggg ggtaactcct atgctaagga ttatcatatc
      901 cgtctttatt tctctgttgg cggtttttat gatggttttt ccgtcggggg aatcggtgat
      961 ttcctccgcc gcttcgccca gaataagatt tatacctttg cttctaatgt agttatggac
     1021 ttcaaccgcc gtatcaaaat ccaaagaacc tataacatgg gaagccttct cgagaactgt
     1081 gacctcgagt ccccttaaat ataggttctc tgccatttca aggcctatat atccgccccc
     1141 gattaccgct gcgctgcggg gtgaatattc ggcgatgtat tctgttattt tgtctgtgtc
     1201 gggaatattt ctaagggtaa aaacattttt tctgtttata cccttaatgt tcgggataat
     1261 cggttttgcg cccggtgaga gtatcagttt gtcgtagctt tcttcgtact cggtgttgtc
     1321 tttaaggttt ttaatatgga caacctttct ttgcctgtcg attttaaccg cttcgcttaa
     1381 aacccttaca tcgattttga atcgattata aaagctttcg ggcgtttgca aaaggagctc
     1441 ttctctttct ttgatttctc cgcctaggta atacggcaga ccgcagtttg cgaaggatat
     1501 gtacggacct ttttccaaaa ggattatttc tgccgattcg tcaagccttc tgagtcttgc
     1561 cgccgcggaa gctcctccgg caactccgcc tatgattacc gttttcaatt tatcatgccc
     1621 ccttatctta ccgtaaatcc ggtccaggac cttattccgc caatgttggt cacatccgta
     1681 tagccttttc tgctgaactc agcgcaggct ctcctgctgc gggcaccgct cttggaatac
     1741 acaaatattt tggcatcctt gtcgtctatt atatcaacaa tcgcgtctgc ctcgtccacg
     1801 ggaatattaa tgctgcccat tatatgtccg ctggcgtatt cttctcttga gcgaacatca
     1861 attagaatga ttgacgggtc tctttcaagg gcttctcggg cttgggccat tgtaatgtta
     1921 ttgaatctag ccgaatatct gccaaacatt aatggcgctc cccccgatct gttcacatca
     1981 taatttctgt tgtccatatt atccctacca aagtcaaagt gaatagcact aatgaaaagt
     2041 aaatatgtgt ttactcacgg gttatttcgg aaatctctca aaattggttt ttcaaaagct
     2101 actttgacga atttttaaca tttccgaaaa atttgttaaa aaaatgatta tctctttaat
     2161 tactaatata atgatggcaa gatatttctc tatgacattg tcacatatta cacttatcac
     2221 ttgtttcggg cggcaattca tggaaaattt gcccaattta atatgagttt tttagaaatt
     2281 agaaactttg gaagagctaa aaaaacatat actctttcaa cattagattg aacaatacca
     2341 caaattgtaa attttttcaa gctgcaactg taaatcatta ttaattctat attttataac
     2401 aataaagtgt gaggatagaa aaacactttt gttaaagttc catagtatag tattatatgg
     2461 agaagttttt ccataatccc tcgaaacagg gaccaaattt gcaacagaat cttacaaaaa
     2521 tccgtcatac gatatcactt tacatcaaac caaataaaaa taatgggact ttttgacagc
     2581 gagaacaaat tttttaattt tgctgtttta atatcgtttt actggccggg taaaagctgc
     2641 cgccgcactt gaaatgcgta gactcgtgtt atataatgtt gaagattgaa attcaagcca
     2701 cgcaagggaa attatacgga aacaggtgca attaaatgaa agtgtataaa tctgccgtaa
     2761 tcggctgcgg caacattcac agaaaccaca ttaaaggaat taacgacagt ccgctgggaa
     2821 aactgaccgc ggtttgcgat atagaccagg aggctgccga caaatccgcg tcggaaaacg
     2881 gcgttcccgc tttttacagc cttgatgagc tattaaagtt cggggatttt gatgttcttc
     2941 acatctgcac tccccattat ctccacgccc ccatggctat taaggctatg cgtcagggca
     3001 aacacataat atgcgaaaag cccatggcaa tccactccgc agacgcaagg gaaatgatta
     3061 aggtcgctga agaaacgggc gttcagctcg gcgtctgctt tcagaaccgc tacaacagct
     3121 catcgattta tgtaaaggag ctattggaaa gcagcaggat gggcaaaatt ctaggcgccc
     3181 gcggaattgt tacttgggac cgctcagaag cctactatgc aagcggtgaa tggagaggca
     3241 aaatcgcgac tgaaggcggc ggagtgctca taaatcaggc aattcacacc cttgaccttt
     3301 tgcagtggtt tgtggattcc cccgttaaag agcttaaagc aagcatttct acaaagaggc
     3361 tccagggcgt tgtcgaaacc gaggataccg cggatgcatt aatcttcttt gaaaacggcg
     3421 tcaggggcgt tttcttcgcc agcctttgct atacctcaaa ctcccccgtt atgctcgaac
     3481 tcgagtgcga gaacggctcc atactcctgg ctgacaagct gaccgttaag ataaacggtg
     3541 aggaggatat cgtcctcgac atcaacaggc catcctgcga aaaggattac tggggcagcg
     3601 gccatacttg tctgatacat gacttctaca aatcgctttc cgaaaacaag ccttttgcga
     3661 ttgacggcga ggaaggcatt aaggcagtcg aaattgtgga ggcaatttac gattacgcaa
     3721 gaaaatagtg catataataa cctcagcctt attttacggg taaaagcgga tagaataagg
     3781 ctgagttgaa agtcaagcgg gcgaattcct atggggaaaa agtggcttga cttttttatt
     3841 gatttttaac aaagaaaact atcgccggct catcagtttg tataaaaaac cgatgcttaa
     3901 gcgatgtttc ctcctttcaa gcctattcta agtagagtta gtttgattgt atacccgtat
     3961 gcttaaattt agcttaaaat accaaggagt cttataaaat gacattagtc gagctttttg
     4021 acaagaaccc tgtcgaaaat atcataagct cccttgtgct gcagcctcag cgcgttatat
     4081 ttgtggggcg aaaagagatt atggcggggc ataaaaggat aatcgagaag ctttttaatg
     4141 aaagaaggct taaaaccgat attcactttt tgcaccccga caggaactct ttggactgcg
     4201 ctgtggagct tttgagcgaa atacagaaaa aattccccga ctgcgagttc gatttgacag
     4261 gcggggacga tttaatgctc ttggctatgg gggttctgat ggaaaaaagc ccgacaaagc
     4321 cgaaagtaca cagatacttt atcagcgaca ggagcattgt gggcattgat atagacggct
     4381 cagaaacaag aaaaactccc ttttatttaa gcgttgggga aaccataaat ctctacggcg
     4441 gcagcgttct caaaagcttt aaatacgagt cccgctggga catgcccgag gattttaaag
     4501 acgatacgga caagatgtgg gggctttgca ggaaagatac cgggctttgg aacagactga
     4561 cgggtgtgct tgccgacttt gagcatatga aagaaaagag ggacagcctt gaaacggtgc
     4621 tatccctttc tgaaattcgg tcgcatctga gcaacaagaa tcaaagcttt gacgctgttg
     4681 agaaattcct gcaaagtctt tgttcgctga aactgattca ggataccggc tcggacaacg
     4741 catttttaag atacagattt aaaaatgagc tgattcgcca ctgtcttgta aaggcgggaa
     4801 cggtgctgga gctgaagatt ctgatgattg cgagaggaat tcgcgatgaa aaggacgagc
     4861 ccttctttaa cgacgcgctg cagggcgtga atatagactg ggacggcaaa cggcacagct
     4921 cgccctttat agccgaaacg gaaaatgaag ttgatgttat actgatgcga gggcacattc
     4981 cgattttcat ttcctgcaaa aacggcttta cggatgatga ggagctttat aagctgaaca
     5041 ccgttgcgga aaagttcggc ggaccctatg ttaaaaaggt gctgattgcc acaaagctga
     5101 caggacgaaa cagaagtctt gaatacttcc gccagcgggc aaaggatatg ggaattacgc
     5161 tgattgaaaa cgctcatctt atgaacgatg aggatttggc ggaaaaaata aaggggttgt
     5221 agtctaagct gcaacccttt ttattttgaa aaaatctttt tgcccagttc ctctgcctgt
     5281 ctaattgctt cggagtctga aattcctttg tctgtttccc ccatatggac gctgcctgca
     5341 agattgattc caagcagcgc aaaagctctg tccgtctgct tttttatcag ctctacaggc
     5401 tgctttgcct ttctgccgct gacggtgagg agaactcccc tgccatcatt ttttctttta
     5461 ggctcttctt tcctgaccac ataggcttcg taaaaccttt gagttcggtc aaagacggct
     5521 tttaatggag cggggacatt atagtggtat acgggagtgg cgattatcac gacatccgcg
     5581 ttttgcattt tgtcaaagac ctcatcaagg tcgtcaaaga cgcaggtatt ttccttgccg
     5641 cacgccttgc agtcgttgca gggtttgggc aataaatcaa ataggtttac tgtgtcaacc
     5701 gagagatttt cgggggcaga cacaaaaaag ccgtcaagca gcttttttgt atatccgttt
     5761 ttgtttggcg aggaaaacaa aacaaagacc ttcatgccgc tccccctttt ttcattaaaa
     5821 caaaagccgg gaccttgccc ggcttgaatt tcgaagatta ttcctcgttc ttctcctcgt
     5881 cgtcctcgtt gacgatttcg aacttgtcaa aaacctctcc gcaattgggg caggtcaact
     5941 gctcatcgcc gtcgaatatg cttgagtcaa agcatactat ctctttgcag gacgggcact
     6001 caagctcata aaggtcgtcc tcgtcaaagt cgtcgcaaat atcgcagtca cattcttcat
     6061 cgtcatatac ataggactcg agggcgtcca aatcttcgtc aacagcgtca agctgctctg
     6121 tgataagggc gatttcgtcc tcattgtctg ctactgtaag tgcaaggtca tcgagcaaat
     6181 caataatggc attgattacc ttagtttctt tattctcagg gtcaagaccg agtccctcag
     6241 caagcccttt taaataagcg gctttttcgg taagattcat aacattaccc tccatattat
     6301 ttttttcctc aaaaccggca ggcggctttc gtctgtactt tttacagata tcaagcagat
     6361 tatactctga aattaaacca aattttgtct aattcagatg tcaatgcttt aagctcttga
     6421 catatactcg cctgtacggg tgtcgacctg gataagctcg ccctcttcta taaagagcgg
     6481 aaccttaatt tcggcgcctg tttcaagaac tgccggcttt aaggtgtttg tagcggtatc
     6541 gcctttgaat cccggctcag tcttggcaac tctgagctct acgaagttgg gaggctcaac
     6601 gccgaaaaca ttgcctttat aagaaagcac tttgcaaacc atgtcttctt ttacatattt
     6661 aaagttatcg tcgagcttat cgccgt
//
LOCUS       DAONVQ010000103         6647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_8675_length_6647_cov_4.384557, whole genome shotgun sequence.
ACCESSION   DAONVQ010000103 DAONVQ010000000
VERSION     DAONVQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8675_length_6647_cov_4.384557"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLD98_06990"
     CDS             complement(<1..213)
                     /locus_tag="PLD98_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA06026.1"
                     /translation="MKSSRLRRLVAAPYLVWIVGFTILPLITIIYYAFTERGTNSFTF
                     KNILAIRDPIHFNALVLSLWVAFLSTL"
     gene            complement(203..1279)
                     /locus_tag="PLD98_06995"
     CDS             complement(203..1279)
                     /locus_tag="PLD98_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA06027.1"
                     /translation="MEKDIIIDLKDISVSFDGEPVLNNINLYIRDNEFITLLGPSGCG
                     KTTTLRIIGGFLKPDTGRVIFEGKDITDLPPNKRQLNTVFQNYALFPHLTIAENIAFG
                     LKIKKKSKEYIKDKIKYALKLVNLEGFENRMPQSLSGGQQQRIAIARAIVNEPKVLLL
                     DEPLGALDLKMRQSMQYELIRLKNELGITFVYITHDQEEALTMSDTIVVMNKGNIQQI
                     GTPEDIYNEPKNAFVADFIGESNIIDGIMLEDKLVEILGAKFPCVDTGFGRNKPVDVV
                     IRPEDVDLVEPEKGIIKGVVTSLLFKGVHYEMDVLANGYEWLVHSTDMVPVGREVGIF
                     VDPYNIQIMHKPISEDEEASSDEE"
     gene            complement(1707..2162)
                     /locus_tag="PLD98_07000"
     CDS             complement(1707..2162)
                     /locus_tag="PLD98_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06028.1"
                     /translation="MYITDSLPNTLRTWLTMHLSSKGYVFMTEFPNMYKQVPLRVPTI
                     VIGVKSSEIKQKKDENGENVPGVKEMTAALDITIHMPVRQGGMTCHKIFSEIADLLLF
                     NTNLDIVEIGSGKIKYMRITESLRMPGFVKIKADITADPEKEYTSPLLL"
     gene            complement(2149..2493)
                     /locus_tag="PLD98_07005"
     CDS             complement(2149..2493)
                     /locus_tag="PLD98_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06029.1"
                     /translation="MAVNAILSKYGRDICIKDTTGWQSKIFKAFIQPLRYKNKMYLEG
                     TPTEIGVMDSGYYLYIGPPEHSFEHLAPGFYLESMGKRYVIDRFEKIYKGGRVFYIWA
                     VLKDAEEQRVYY"
     gene            complement(2513..2875)
                     /locus_tag="PLD98_07010"
     CDS             complement(2513..2875)
                     /locus_tag="PLD98_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06030.1"
                     /translation="MISQWEVLERLRQTVTLDDDEAAAALPFCKAAAQSFERKLRKKE
                     DCANQLIIMLCAMTALYKFLLARSAADTEFTGFRAGDVTVKKSYKEALEAAEKLKDDA
                     FEQAAHLLKDDEFVFRRF"
     gene            complement(2931..3944)
                     /locus_tag="PLD98_07015"
     CDS             complement(2931..3944)
                     /locus_tag="PLD98_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HQA06031.1"
                     /translation="MSGFEKIRLEKGMYAAKGGFTAALEALDPSENYKGTALEGLDAY
                     QRQLKRFDIKVAGPASDRVEKFFSTADSAALFPEYVSRAVRQGMEEANILPKIVATVT
                     DIDALDYRTITSVPSEDDKELKMVAEGSFIPETTIKTKENLVRLKKRGRMLVASYEAI
                     RFQRIDLFTVTLKQIGAQIARSQVNDALAAIVLGDGNDNPAEIIRKSEGNELTYEDLV
                     DFWSRFDPYQLNTLLVSGETMRKILSMKEFKDSAAGLSFHATGKMVTPLGAELIKVPN
                     FEEDFLIGIDRNCALEMVRAGGVVTEYDKLIDRQLERAAITCTTGFSKIFDDACKVFV
                     FGE"
     gene            complement(3960..4304)
                     /locus_tag="PLD98_07020"
     CDS             complement(3960..4304)
                     /locus_tag="PLD98_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06032.1"
                     /translation="MSVSLKGFNEKVITLQAVDGLKAGDAVSLSGNLEVSPASEGTDI
                     FGFAISVMDGYAAVQVSGFITVPCEGIEDAYVGYRAIQGADEGKIALAESGRKVFVVS
                     VDKAAGKIGIIL"
     gene            complement(4321..5289)
                     /locus_tag="PLD98_07025"
     CDS             complement(4321..5289)
                     /locus_tag="PLD98_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06033.1"
                     /translation="MKKEKRFDDAVFSPIESDMEKINCFSLKPLKEEDVFVFSVILCD
                     NEIDRDYERFSADCLHGLKELFKGVTGIFDHNMRAENQTARIFDTEIVTDSDRTTAAG
                     EPYTYLKAHCYMLRSEKNKNLIAEIEAGIKREVSISCSVKRAVCSVCGKENGACSHRP
                     GSEIEGKVCHRILCDAADAYEWSFVAVPAQKGARVTKQYTKTQRGEISLTEVLKKLDI
                     DKDELRLSSAEALQLKSYIENLKSLADDGEHYKSALVKKALSLSALKLPKMDTAVFKE
                     VLLKMPARDIEKIVKGFSEDEPDSVFAPQLSSDSCAKSSDNSGFRI"
     gene            complement(5286..6467)
                     /locus_tag="PLD98_07030"
     CDS             complement(5286..6467)
                     /locus_tag="PLD98_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HQA06034.1"
                     /translation="MGLFSKKEKPSASAVSSPQTRQAGQSSFFDDYKSMYSFHGDISL
                     YKTLRESVPIIDAGIRKLARLIGSFEVRCDDSFAQREIRDFLQNVVVDGSSRGINSFV
                     YAYFEQLLTYGTAVAEILPSKGSPRDMSLYRADLRDIVLMRDKNDSSKTLVCVSRGSE
                     PVPVKHQELILLSVLNPEAGSLNGNSILKGLPFISSVLMKIYNTIGLNFERVGNVRYA
                     VTYRPKEGSADKLYAKERAMQIASEWSAAMQSGSEIKDFIAVGDVDIKVIGADNQILD
                     SEVPVRQMLEQIVAKLGLPPFMLGLNWSTTERMAAQQTDILTSELEFYRKLLTPVIGR
                     IISTFMRFNGLKGGFEILWDEISMQDEVELSKARLNNAKALEVERRLGAAPDSQKEVS
                     D"
BASE COUNT         1552 a         1712 c         1553 g         1830 t
ORIGIN      
        1 aagggtgctg aggaaagcta cccagaggga gagaaccaga gcgttaaagt gtatggggtc
       61 gcgaatagcg agtatatttt taaaggtaaa gctgtttgtg cctctttctg taaaggcgta
      121 gtagataatt gtaataaggg gcagaatcgt aaagcctaca atccagacaa gatacggggc
      181 tgccacaaga cgccttaatc tgctactctt catcggatga cgcctcctca tcctctgaaa
      241 tcggcttatg cattatctgg atattgtagg ggtcaacgaa tatgcccacc tccctgccga
      301 cgggaaccat atcagtgctg tggacaagcc attcgtagcc gtttgccaaa acatccattt
      361 catagtgcac gcctttaaac aaaagggagg tgacaacgcc ctttatgatg cccttttcgg
      421 gctccacaag gtccacatct tcgggtctta tgaccacatc gacgggctta tttctgccaa
      481 agccggtatc aacgcacggg aatttggcgc cgagtatttc taccagctta tcttccagca
      541 taatgccgtc tattatgttg ctctcgccta taaagtcggc tacaaaagca ttcttaggct
      601 cgttgtaaat atcctcaggg gtgcctatct gctgtatgtt gcccttgttc atgacgacta
      661 tggtgtcgga cattgtaagg gcttcttcct gatcatgggt tatataaaca aaagttatgc
      721 ccagctcgtt tttaagcctg ataagctcat actgcatgct ctggcgcatc ttaaggtcaa
      781 gggcgccgag aggctcatca agcaataaaa ccttgggctc gttcacaatt gcccgagcga
      841 tggcgattct ttgctgctga ccgccgctga gcgattgcgg catacgattc tcaaagccct
      901 caaggtttac aagcttcaag gcatacttta ttttgtcttt gatatactct ttgctctttt
      961 tcttgatttt aaggccgaag gcgatatttt cggcgattgt caaatgcgga aacaacgcat
     1021 aattctgaaa gaccgtatta agctgccttt tgttgggagg caggtcggta atgtccttac
     1081 cttcaaatat cacccgtccg gtgtcaggtt ttaaaaaccc gcctataatc cgaagggttg
     1141 tggtctttcc acatcccgac ggcccaagca gggtgatgaa ttcattgtcg cgaatataaa
     1201 ggttgatgtt gttcagaacc ggctcgccgt caaaagacac ggaaatgtct ttcagatcga
     1261 tgatgatatc tttttccaat tatgcagccc ctctcgaaaa aaattgcgtt tacgacataa
     1321 gccgttgaca aattacaata tagagtttat ttaacggaat gtcaataatt ttaattaaaa
     1381 tatattcccc cgtgaacttt tgtaactcta aggctcggct cgatttttta ggcacgccga
     1441 aattgctctt gagagagcca aaaatgaaga aaaaatcgag gaagttttat tattgggctt
     1501 actgaatatt tatgaaaaat attccaaaat atcctgtatt tttctgcaaa cctttatttt
     1561 tttacaaaat gcggcgagcc gtctttatat agtttgaata gggggcgcga aacttatacc
     1621 taaagtttat aaaaacaaaa aaccgccctt taaaaagagc ggttttaatg tgtgaagggg
     1681 tttatttttt tattgcgggg cggaaattaa agaagtaagg gagaagtgta ctctttttcg
     1741 gggtcggccg taatatccgc cttgattttt acaaagccgg gcattctgag gctctccgtt
     1801 atcctcatgt actttatttt tccgctgccg atttccacaa tatcaagatt tgtgttgaaa
     1861 agcagaaggt cggcgatttc ggagaaaatt ttgtggcagg tcattccgcc ctgacgaacg
     1921 ggcatgtgga ttgttatgtc taaagcggcg gtcatttcct tgacaccggg gacattctcg
     1981 ccgttttcgt cctttttctg ttttatctca gacgatttaa cgccgattac gattgtgggg
     2041 actcttaacg gaacctgttt atacatattc ggaaactcgg tcatgaacac atagccctta
     2101 gagctaagat gcattgttag ccatgttctt aaagtattgg gcaatgaatc agtaatatac
     2161 acgctgctcc tccgcgtcct tcaggacagc ccagatatag aaaaccctgc cgcccttgta
     2221 gattttttcg aatctgtcta tgacatacct tttgcccatt gactcaagat aaaagccggg
     2281 ggcaaggtgc tcaaagctgt gctcgggggg acctatataa agatagtagc ccgagtccat
     2341 gacgccgatt tccgtcggag tgccctcaag atacattttg tttttgtatc tcagcggctg
     2401 aatgaacgcc ttgaaaattt ttgactgcca gcccgtagtg tccttaatgc agatgtccct
     2461 gccgtatttt gaaagtatag cgtttaccgc caaaaaaaac acctccccgc ggctaaaagc
     2521 gcctgaatac gaattcgtcg tcctttaaaa gatgggcggc ctgctcaaag gcgtcgtctt
     2581 taagtttctc cgcggcctca agagcctcct tgtaggactt tttaaccgtc acatctcccg
     2641 ccctgaagcc cgtaaactca gtgtccgccg ctgagcgggc aagcaggaat ttataaaggg
     2701 cggtcatggc gcaaagcatg ataataagct gatttgcgca gtcctctttc tttctgagtt
     2761 ttctctcaaa gctctgagcc gccgccttgc aaaagggcag agccgctgcc gcctcatcgt
     2821 catcgagggt cactgtttgt ctcagtctct caagtacctc ccactgactt atcatcaaat
     2881 cacctcttgc cttttaaggg agcctgctct atgcaggctc ccccttttta ctactcgcca
     2941 aacacgaata ccttgcaggc gtcgtcaaat atctttgaga agcctgtggt gcaggtgatg
     3001 gcagcccttt caagctgacg gtctatgagc ttgtcgtact cggttacgac gccgccggct
     3061 ctgaccatct caagggcgca gttcctgtca atgccgataa ggaaatcctc ctcgaaattg
     3121 ggaaccttaa taagctcggc gccgagaggt gttaccatct tgcccgtggc gtggaaggaa
     3181 agacccgccg cggagtcttt aaactccttc atgctgagta tttttctcat tgtctcgccg
     3241 gaaacaagaa gggtgttgag ctgatagggg tcgaacctgc tccagaaatc caccaaatcc
     3301 tcatatgtaa gctcgttgcc ttcgctcttt ctgataatct cagcggggtt gtcgttgccg
     3361 tcgccgagaa caattgcggc aagggcgtcg ttaacctgac ttctcgcaat ctgagcgcct
     3421 atctgcttta aggttacggt aaacaggtcg attctctgga aacggattgc ctcataggag
     3481 gctaccagca tcctgcccct ctttttgagg cggacaaggt tttcctttgt ctttatagtg
     3541 gtttcgggga tgaatgagcc ctccgctacc atcttaagct ccttgtcgtc ctctgagggt
     3601 acggaggtga tggttctgta gtcaagggcg tcaatgtccg ttaccgtcgc cacgattttg
     3661 ggaaggatat tcgcctcctc catgccctgc cttaccgcgc gggaaacata ttcggggaag
     3721 agagccgcgg agtcggcggt gctgaagaac ttttcaactc tgtcgctggc gggacccgca
     3781 acctttatat caaacctttt aagctggcgc tgataagcgt caagaccctc aagcgcggtg
     3841 cccttgtaat tttcggaggg gtctaaagcc tcaagagccg cggtaaagcc gccctttgcg
     3901 gcgtacatgc ctttttcgag ccttattttt tcaaaacctg acattccttt tctcctctct
     3961 taaagaataa ttccgatttt tcctgccgcc ttgtcgacgc tgacgacaaa gacctttctg
     4021 ccgctttcgg caagggcgat ttttccctca tcagcgccct gaattgcgcg gtagcccaca
     4081 taagcgtcct ctataccttc gcagggcact gtgatgaagc cgctgacctg aaccgcggca
     4141 tagccgtcca tgacgcttat ggcaaagccg aaaatatcgg tgccttcgct tgcgggagaa
     4201 acctccaaat tgccggagag agaaaccgcg tcccccgcct ttaagccgtc tacggcctgc
     4261 agggtgatta ccttttcgtt aaagcctttg agtgaaactg acaattaaac catcctttct
     4321 ttaaattcta aagccgctgt tgtcgcttga tttagcgcac gaatcggagc ttagctgcgg
     4381 agcgaaaacg gaatcgggct catcctcgga aaagccctta acaatctttt cgatgtccct
     4441 tgcgggcatc tttaagagaa cctccttaaa aaccgccgta tccatcttgg ggagcttcag
     4501 agccgagagg ctcagcgcct ttttaacaag ggcgcttttg tagtgctctc cgtcatcggc
     4561 aaggctcttc agattctcta tgtagctttt gagctgaagg gcctccgcgg agcttaaacg
     4621 cagctcgtct ttatcgatgt cgagcttttt aagaacttcg gtcaagctga tttcacctct
     4681 ttgcgttttt gtgtactgct ttgtaaccct cgcccctttc tgagcgggaa cggcgacaaa
     4741 cgaccactca taggcgtccg ccgcgtcgca gagaatcctg tggcagacct tgccctcgat
     4801 ttcagagccg ggtctgtggg agcaggcgcc gttttccttg ccgcagacgg agcatacggc
     4861 cctttttacg ctgcatgata tgctgacctc acgcttaata cccgcctcaa tttctgctat
     4921 aaggttcttg tttttttcgc ttcgcagcat atagcagtgc gccttgagat aggtgtaggg
     4981 ctcacccgcc gcggtggttc ggtcgctgtc cgtcacaatc tcagtatcaa aaattctcgc
     5041 ggtctggttt tccgccctca tgttatggtc aaatatgccc gtcacgccct taaaaagctc
     5101 tttaaggcca tgaaggcagt cggcagaaaa ccgctcgtag tccctgtcaa tctcgttgtc
     5161 gcagagaatg acgctgaaga caaagacatc ctcctcttta aggggcttta gagaaaagca
     5221 gtttatcttt tccatgtccg attctatcgg ggaaaagacg gcgtcatcaa accttttttc
     5281 ctttttcaat cggaaacctc cttttgactg tcgggggcag cgcccagcct tctttcaacc
     5341 tcaagggcct ttgcgttgtt aagccttgcc tttgacagct ctacctcgtc ctgcatgctg
     5401 atttcgtccc acagaatttc aaagccgcct ttaagaccgt tgaaccgcat aaaggtgctt
     5461 ataatcctgc cgattacggg ggtcaggagc tttctgtaga actccagttc gcttgtcaaa
     5521 atgtcggtct gctgagccgc catgcgctcg gttgtggacc agttaagccc cagcatgaaa
     5581 ggcggcagac ccagcttcgc gacaatctgc tccagcatct gccgtacggg tacctcgctg
     5641 tcaaggattt ggttatcggc gcctatgacc ttaatgtcca catcgcccac agcgataaag
     5701 tcctttatct cgctgccgct ctgcatcgcc gccgaccact cggaggctat ctgcatagcc
     5761 ctttccttgg catagagctt gtcggcggag ccttctttcg gtctgtaggt cacagcgtag
     5821 cggacattgc ccacacgctc gaaattaagc cctattgtgt tgtaaatctt catcagcaca
     5881 gagcttataa agggcagacc tttaagtatc gaattgccgt taagactgcc cgcttcggga
     5941 ttgagaaccg ataaaagtat cagctcctga tgctttacgg gaacgggctc cgatcctctt
     6001 gaaacgcaca caagggtttt ggagctgtcg tttttgtccc gcatcagaac aatatcccta
     6061 aggtcagctc tgtaaaggga catatccctt ggggagcctt tgctcggcag aatctccgcc
     6121 acagcggtgc cgtaggtcaa aagctgctca aaataggcgt acacaaaact gtttatgccc
     6181 cttgagctgc cgtcgacaac cacattctga aggaaatctc gaatctccct ctgggcaaag
     6241 ctgtcgtcgc agcgcacctc aaagctgccg atgagccttg ccagctttct tatgcccgcg
     6301 tcaataatag gcacgctctc cctgagcgtc ttgtaaaggc ttatgtcgcc gtgaaagctg
     6361 tacatgctct tgtagtcgtc aaaaaagctg ctctgacccg cctgtcttgt ctggggtgag
     6421 gacacagccg acgccgacgg cttttctttt ttgctgaaga gtcccaaatt ctcacctctt
     6481 gccttttaat taccgaacca tcgttttgca aacctttata tccgcccctt tgggctcgtc
     6541 agaacccgaa atcctgacgc cttgaacgct cggaggcgat ggcgaaaaag ccctcatccc
     6601 cgccgccttc cagaacggtg cttacaaagt agcgcatatc gtccatg
//
LOCUS       DAONVQ010000104         6598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_8748_length_6598_cov_4.659178, whole genome shotgun sequence.
ACCESSION   DAONVQ010000104 DAONVQ010000000
VERSION     DAONVQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8748_length_6598_cov_4.659178"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLD98_07035"
     CDS             complement(<1..360)
                     /locus_tag="PLD98_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HQA06035.1"
                     /translation="MELKLKDRDSCQFDMLALGEVMLRLDPGEGRIKTTRSFKAWEGG
                     GEYNVARGLKRCFGLRTTVVTAFADNEIGRLIEDLIFQGGVDTSLIKWVPYDGIGRTV
                     RNGLNFTERGFGIRGAVG"
     gene            complement(383..1240)
                     /locus_tag="PLD98_07040"
     CDS             complement(383..1240)
                     /locus_tag="PLD98_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQA06036.1"
                     /translation="MFTYHHPNLKGRTAVITGGGGVLCSGFAKDLAKQGMKVAVLDLK
                     EEAAQAVADEIKKDGGEAIAVQCDVLSPESLENARKIVTEKYGVCDLLINGAGGNSPL
                     GTTTKEKLELSDLAEKAEGVKTFFDLDPKGIEFVFNLNFLGTLLTTQAFAKDMAGREN
                     AAIINVSSMNSYRPLTKIPAYSAAKAAVSNFTQFMAVHFSDVGIRVNAIAPGFFATNQ
                     NRALLFNEDGSLTPRSAKILAHTPQNRFGVPEDLTGALLFLADEAYSGFITGVILPVD
                     GGFSAYSGV"
     gene            complement(1306..2172)
                     /locus_tag="PLD98_07045"
     CDS             complement(1306..2172)
                     /locus_tag="PLD98_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HQA06037.1"
                     /translation="MAEFILSAFADEAGGGILEQVKALRENGFTHIEPRGLDHGNISK
                     YTPEMCKELKKVLDDNGIKVSAIGSPFGKINIKDDFEEHFELFKVNIENANILGTDKI
                     RIFSFYFDEDDSYEEYRDEVFERLEKFCDYALAHGVWCCHENERGIYGDNDTRCLDIL
                     KTLEGKIKGVFDPANYILCGVEIMPAYRMLEPYIEYMHVKDARFSDGKIVPAGKGDSH
                     YVELIREFDKKDGERILTLEPHLKVFKGLAELEADSGTARKLDDYTYSTNREAFDAAA
                     DALKEVIRLARA"
     gene            2842..3846
                     /locus_tag="PLD98_07050"
     CDS             2842..3846
                     /locus_tag="PLD98_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA06038.1"
                     /translation="MKKLHKIIAVLSILALVLSSAACAKKDAEPSDTAPAGGSAKYTV
                     GIVQLIQHVALDAATKGFRDYLTEEFGDNITFLEQNAQGDPNTCATIANGFVSSKVDL
                     ILANATPALIAAAAATSEIPILGTSITSYADAFKIQDFNGVVGGNVSGTSDLAPLDQQ
                     AQMILDLFPETKKVGIIYCSGEPNSIYQVNIVKEYLEGKGVEVTLYPFADSNDIAAVT
                     TTAAKNSDAIYIPTDNAAASNAKIIDNICRPAKVPVIAGEQGICRGCGVATLSIDYYD
                     LGYTTGKMAAKILKGEADISTMPIEYAPKFTKMYNAEICEDLGITIPEGYEPIPPESE
                     "
     gene            3951..4886
                     /locus_tag="PLD98_07055"
     CDS             3951..4886
                     /locus_tag="PLD98_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA06039.1"
                     /translation="MNLLSQLPGAVAQGLIWGIMAMGVYITYRILDLADLTVDGSFCT
                     GAAVCVMMILNGHSVWVAIICAFLAGMLTGLATGLLHTVFGISPILAGILTQLGLWSV
                     NLAIMTRPNQSISTNNYKLLVALRFLDDVKKGLKPFYNHPIFVVGIFVIVIIAVLYWF
                     FGTELGCSIRATGANINMARAQGINTNVNKIIGLMLSNGMVALSGSLMAQYQNFADIN
                     MGRGAIVIGLAAVIIGEVLFSKIFRNFALKLLSVVLGSIIYYIVMQTVLWLNIDANYL
                     KLFSALVVAVCLGMPYLKAKYITPIQQRKRGKSNA"
     gene            4879..5673
                     /locus_tag="PLD98_07060"
     CDS             4879..5673
                     /locus_tag="PLD98_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA06040.1"
                     /translation="MLELINISKTFNPGTVNEKLALDRLSLTIKDGEFVTVIGGNGAG
                     KSTMLNAVAGVFFVDDGRIIIDGNDVTRLEEHRRAKYIGRVFQDPMMGTAASMGIEEN
                     LALANRRGQFRGLKWGITHKERQFYKEQLATLGLGLENRLTTKVGLLSGGQRQALTLL
                     MASLKKPKLLLLDEHTAALDPKTAAKVLEATEEIVRRNNLTTLMITHNMRDAINHGDR
                     LIMLYEGRIAVDVSGEEKKKLTVEKLLEMFTTASGSDEADDKLLLS"
     gene            complement(5741..>6598)
                     /locus_tag="PLD98_07065"
     CDS             complement(5741..>6598)
                     /locus_tag="PLD98_07065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQA06041.1"
                     /translation="LLEYDGAINLMKGGIECAHMVTTVSPTYAKEILDPWYSHGLDSI
                     LLQRAWKIKGILNGIDTDYYNPETDPDIPANYSCLSPENKALNKAALQKEMGLPEKED
                     TPLIGIVSRLVGHKGFDLVKAILEGVLQNEDVQLAVLGSGEWQYESFFKEMHEKHPEK
                     VGVFIGFNPSLAKRVYAGSDLFLMPSKSEPCGLAQMVALRYGTVPIVRATGGLKDSVF
                     EGEEGNGFVFEGYNPEELLKAIDRALRLYEDKKSWRNLRQRAFDSDYSWGKSANEYIK
                     IYKEVIKKY"
BASE COUNT         1572 a         1753 c         1705 g         1568 t
ORIGIN      
        1 gccgaccgcg cctcttatgc caaagcctct ttctgtgaag ttaagaccgt tgcggacggt
       61 tctgccgata ccgtcatagg gcacccactt gataagggag gtgtccacgc cgccctggaa
      121 aataaggtcc tcgataaggc ggccgatttc attgtccgcg aaggcggtga caacggtggt
      181 tctaaggccg aagcagcgtt ttaagcccct tgccacattg tactcgccgc cgccctccca
      241 tgccttgaat gagcgggtgg tcttaattct gccctcgccg gggtcgagcc tcagcataac
      301 ctcgccgaga gcgagcatat caaactgaca ggaatctctg tctttaagct taagttccat
      361 ttgcaacact cccaaaaaga tattaaactc ccgaataagc ggagaagccg ccgtcaacgg
      421 gaaggattac gcccgtaata aagcctgaat atgcctcgtc ggcaaggaag aggagcgcgc
      481 ccgtaaggtc ctcgggaacg ccgaaacggt tctggggagt atgggcgagg atttttgccg
      541 agcggggagt aagggagccg tcctcattga aaagcagcgc cctgttctga ttggtggcga
      601 aaaagccggg agctatggcg ttgactctga tgcccacatc ggagaaatga acagccatga
      661 actgggtaaa gttcgaaaca gccgccttgg ccgcggagta cgcgggaatc ttggtaaggg
      721 ggcggtatga gttcatggac gaaacattta tgattgcggc gttctccctg cccgccatgt
      781 ccttggcaaa tgcctgagtt gtaaggagag tgccgaggaa gttgagattg aacacaaact
      841 caatgccctt ggggtcaagg tcaaagaatg tctttacgcc ctctgccttt tcggcaaggt
      901 cggaaagctc cagtttctcc tttgtggtgg tgccgagggg ggaatttccg cccgcgccgt
      961 ttataagaag gtcgcatacg ccgtattttt cggtgacgat tttccttgcg ttttcaaggc
     1021 tctcggggct taatacatcg cactgaaccg cgattgcctc gccgccgtcc ttcttaatct
     1081 cgtcggcgac agcctgagcg gcttcttctt taaggtcgag aacggcgacc ttcatgccct
     1141 gcttcgcaag gtccttggca aagccgctgc agagcactcc gccgccgccc gtaatgacag
     1201 cggtgcggcc ctttagattg ggatgatgat atgtaaacat aattacctcc tgaactcgcg
     1261 tttttaaagt cagagagcct tatcccagag gataaggctt tagttttaag ctcttgcgag
     1321 ccttatgact tccttaaggg cgtcggcggc agcgtcgaat gcctctctgt tggtgctgta
     1381 agtgtagtcg tccagttttc ttgctgtgcc gctgtctgcc tcaagctcgg caagaccctt
     1441 gaataccttg aggtgaggct caagggtgag gattctctcg ccgtctttct tgtcaaactc
     1501 tctgatgagc tccacataat gagagtcgcc cttgcctgcg gggactattt ttccgtcgga
     1561 gaaacgggcg tccttaacat gcatgtactc aatgtaaggc tcgagcattc tgtaggcggg
     1621 cataatttca acgccgcaga ggatgtagtt cgcggggtcg aatacgccct taatcttgcc
     1681 ctcaagggtc ttaagtatat caaggcagcg ggtgtcgttg tcgccgtaga tgcctctttc
     1741 gttctcgtgg cagcaccata cgccgtgggc gagagcgtag tcgcagaact tctcgagtct
     1801 ctcaaagacc tcgtccctgt actcctcgta gctgtcgtcc tcgtcaaaat agaagctgaa
     1861 gatgcggatt ttgtctgtgc cgaggatgtt ggcgttttct atattaacct taaagagctc
     1921 gaaatgctcc tcaaagtcgt ccttaatgtt gattttgccg aagggcgagc cgattgcgga
     1981 gaccttgatg ccgttatcgt caagaacctt tttaagctcc ttgcacattt cgggtgtgta
     2041 ttttgaaata ttgccgtggt cgagtccgcg gggctcaatg tgggtaaagc cgttttccct
     2101 aagggctttt acctgctcaa gtatgccgcc gccggcctca tcggcgaacg cggaaagaat
     2161 gaactctgcc ataaaatttc cccctaactg attttaaagg agccgaaaaa aggcccccga
     2221 atcctttcgt aaatatatta cagtgaatac gctccggggt caagtaaact aaggaaaaat
     2281 ttgctgatag ggcaaatacc gactcgctga ttccagggta ttttagccga taacaatgag
     2341 gagcgtcgcc ccgcccgaac ccgccgccga gagagttcgt ccgacgcaga gggttgtccg
     2401 ctccctctgc cccgccgaag gggagtgccg ccccgatgac gaggagaccc gccgccgagg
     2461 gaattcgtcc gacgcagagg attttccgct ccatctgtcc cgccgagggg gtgcgccgcc
     2521 gcccgaaccc gccgccgtgg gggcgctgct cccgccgacc cgagggaggg agtgtccgcc
     2581 gccccgatga cgaggagacc cgtcgcccgc cgcaagacga aaaatctgtt cccgctcccc
     2641 ggacttcgga cgcagagggg aggataattc taaggcaagg ctcgctcagc agggcagcgt
     2701 cgaaaaaaga aataaaaatt ataaatttct tcttgacatt tattctcaga tgtatataat
     2761 taaagtcaac atcactttac cgcttttatg cgacaaagcg acaaagcgac aacgcgctaa
     2821 attgaatgag aggtagaaaa tatgaaaaag ctacataaga ttatcgcagt gctctcaatt
     2881 cttgcacttg tgctttcctc cgccgcatgc gcaaagaaag acgcagagcc ttccgacacg
     2941 gctcccgccg gcggcagcgc aaagtacacc gtaggtattg tgcagctaat ccagcatgtt
     3001 gctcttgacg ctgccacaaa gggcttccgt gattatctga ccgaggagtt cggcgacaac
     3061 atcaccttcc ttgagcagaa cgcccagggc gatcccaaca cctgcgcaac catcgccaac
     3121 ggctttgttt cctccaaggt tgacctgatt ctcgccaacg cgactcccgc tcttatcgca
     3181 gcggccgccg caacatcgga gattccgatt ctcggcacct ccatcacatc atacgccgat
     3241 gcgtttaaga ttcaggactt caacggcgta gtgggcggca atgtatcagg cacatcagac
     3301 cttgctcctc tggatcagca ggctcagatg attctcgacc tgttccccga gaccaagaag
     3361 gtcggcatta tctactgctc cggcgagccc aactccatct atcaggtaaa catcgttaag
     3421 gagtatcttg aaggcaaggg cgttgaggta actctctacc ccttcgcaga ctccaacgat
     3481 atagccgctg ttacaaccac agccgccaag aacagcgacg ctatctatat ccccaccgac
     3541 aacgcggcag cctccaacgc taagataatc gacaacatct gccgtcccgc aaaagttccg
     3601 gtaatcgccg gtgagcaggg catctgcaga ggctgcggtg tggcaaccct ttcaatcgac
     3661 tactatgacc tgggctacac aacgggcaag atggctgcca agatactcaa aggcgaggcc
     3721 gacatttcaa caatgcccat tgagtacgcg cccaagttca caaagatgta taacgccgaa
     3781 atctgcgagg accttggcat cacaatccct gaaggttatg agccgattcc tccggagagc
     3841 gaataatcca tagtatgcca gtgtcttaaa acggcagaga aggcacaaac ttttctgccg
     3901 ttttaagttg ttttttgatt ttgggaggaa ttgttttgtc tttaaatcca atgaacctgt
     3961 taagccagct gcccggagcc gttgcccaag ggcttatatg gggcataatg gccatgggcg
     4021 tttatattac atacagaata cttgaccttg ccgatttgac cgtagacggc tccttctgca
     4081 cgggagcggc tgtctgcgtt atgatgattt taaacggtca cagcgtctgg gtggcaataa
     4141 tctgcgcctt ccttgccggt atgctgacgg gtcttgccac gggacttctt cataccgtat
     4201 tcggcatatc gccgattctg gcgggaattt taacccagct gggtctatgg tccgtaaacc
     4261 ttgctattat gacaaggccc aatcagtcca taagcaccaa caactacaaa cttcttgtcg
     4321 ctctgagatt ccttgacgat gtaaagaagg gtctgaagcc tttctacaat cacccgattt
     4381 tcgtcgtggg aatcttcgta attgtcatca tagccgttct gtactggttc ttcggtacgg
     4441 agctgggctg ttccataaga gcgacgggcg caaacattaa tatggcaagg gcgcagggca
     4501 tcaacaccaa tgtaaacaag attataggac ttatgctttc aaacggcatg gtagcccttt
     4561 cgggcagtct tatggctcag tatcagaact tcgccgacat caacatggga cgcggcgcaa
     4621 tcgtcatagg tcttgccgcc gtaattatag gagaagtgct gttctcaaag atattcagaa
     4681 acttcgcgct caaactcctt tcagtggtgc tcggctccat aatctactac atagtcatgc
     4741 agacggtgct gtggctgaat atagacgcga actacttaaa gctattctcc gcgctcgtcg
     4801 tggctgtctg cctcggaatg ccttatctca aggcaaagta catcaccccc atccagcaga
     4861 gaaagagagg aaagagcaat gcttgagctt ataaatattt caaagacctt caaccccggc
     4921 actgtcaacg aaaagctcgc ccttgaccgc ctctctctca ccattaagga cggggagttc
     4981 gtgacggtca taggcggcaa cggagcgggc aagtccacaa tgctgaacgc cgtcgcgggc
     5041 gtgttctttg tggacgacgg cagaataata attgacggca acgatgttac ccgccttgag
     5101 gaacaccgca gggcgaaata tataggcagg gtctttcagg acccgatgat gggcaccgcc
     5161 gccagcatgg gcattgagga gaaccttgct ctggcgaacc gcagaggaca gttcagaggc
     5221 cttaaatggg gcatcaccca taaggaaagg cagttttata aagagcagct cgcgacactt
     5281 ggactcggcc ttgaaaaccg ccttacaaca aaggtgggtc tcctttcggg cggtcaaagg
     5341 caggccctta ccctgcttat ggcaagcctt aaaaagccca agctcctgct gctcgatgag
     5401 cacacagcgg ctcttgaccc gaaaaccgcc gccaaagttc ttgaggcgac tgaggaaatc
     5461 gtccgccgca acaaccttac aacccttatg attacccaca acatgaggga cgccattaac
     5521 cacggcgaca ggctgataat gctttatgag ggcaggattg cggtagatgt ttcgggcgag
     5581 gaaaagaaaa agctgacagt ggagaaactg ctcgaaatgt tcaccaccgc cagcggctcc
     5641 gatgaagcgg acgacaagct gcttttatct taaagtttag cccggcctga caaggctgaa
     5701 tataaaaaag acggaaatcg aaagatttcc gtctttttgt ttagtacttc tttattacct
     5761 ctttatatat ctttatatac tcgttcgccg actttcccca gctgtagtcg gagtcaaagg
     5821 ctctctgcct gagatttcgc cagctctttt tatcctcata aagtctcagg gctctgtcga
     5881 ttgcctttaa aagctcctcg gggttgtagc cttcaaatac aaagccgttg ccctcctcgc
     5941 cttcaaagac agagtcttta agaccgcccg tagcccttac aatcggcacc gtgccgtagc
     6001 ggagggcgac catctgcgca agtccgcaag gctcgctttt tgacggcata agaaagaggt
     6061 cggagccggc atagaccctc tttgcgagtg aggggttaaa gccgatgaat acgcccacct
     6121 tttcggggtg tttttcgtgc atctccttaa agaagctctc atactgccat tcgcccgagc
     6181 cgaggacggc aagctgcaca tcctcatttt ggagcactcc ttcaagaatc gctttaacaa
     6241 ggtcaaagcc cttgtgaccc acaagccggc tgacaatgcc gattaaagga gtgtcctcct
     6301 tttcgggaag acccatctcc ttttgaagag ccgccttgtt caaagccttg ttctcggggc
     6361 taagacacga gtagttcgcg ggaatgtccg ggtcggtttc ggggttgtag tagtcggtgt
     6421 caatgccgtt aagtatgcct ttaatcttcc acgctctttg taggagtatg gagtcaagtc
     6481 cgtgggagta ccatgggtca aggatttcct tggcgtatgt ggggctgacg gtggtgacca
     6541 tgtgggcgca ctcaatgcct cccttcatca gattaatggc tccgtcatat tcgagaag
//
LOCUS       DAONVQ010000105         6560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_8823_length_6560_cov_4.547118, whole genome shotgun sequence.
ACCESSION   DAONVQ010000105 DAONVQ010000000
VERSION     DAONVQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8823_length_6560_cov_4.547118"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLD98_07070"
     CDS             complement(<1..312)
                     /locus_tag="PLD98_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06042.1"
                     /translation="MKKSLKIIALILSFVILFSSCFRKEPGTEDASSETKIGGETKRP
                     AQQVDRVRLPFSLEDSLNPFFLKGVRNISLVPLMFDSLYVIDEKYMPVPSVALEAVEE
                     GL"
     gene            complement(325..1095)
                     /locus_tag="PLD98_07075"
     CDS             complement(325..1095)
                     /locus_tag="PLD98_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HQA06043.1"
                     /translation="MNILAIGDVVGTQGCEFLRAHLPAIKKLNQIDICIANGENSAQG
                     NGITPSSANYLLESGVDLITTGNHVFRRHEVYEYLNSSVPVIRPANFHHTTPGKGFWI
                     IDRGRFSVAVINLAGTVYMESASNPFDKVDEILNELKDIKNIIVDFHAEATAEKKALG
                     FYLDGRVSAVFGTHTHVQTSDACILPKGTAYITDLGMTGPVHSVLGVEPSLAIEKLRT
                     GMPVRFENPAGKVSMNACIFSIDEKTGKALSVEAITIE"
     gene            complement(1200..2726)
                     /gene="rny"
                     /locus_tag="PLD98_07080"
     CDS             complement(1200..2726)
                     /gene="rny"
                     /locus_tag="PLD98_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HQA06044.1"
                     /translation="MVAGGVILFGVGFALGSVYRRKVAERTIGSATDEANRIVSQAIA
                     QAETKKKEAIIEAKDEIHKLRTEADREIRERRTEIQRQERRLIQKEESLDKKLDALEK
                     KDETLNKKLKTVDERLEELETLKKSQYEMLERISGFSQEQAKEYLLKNLEDSLEHEKA
                     VMLMEHKQKTLEEAEAMAREIISTAIQRCAADHAAEATVSVVALPNDEMKGRIIGREG
                     RNIRALETTTGVDLIIDDTPEAITLSSFDPVRREIARLTLEKLIADGRIHPARIEEMY
                     DKARREVEATIKQTGERAVIEAGINGVHPEIVKLLGRLKYRTSYGQNVLDHSLEVSHI
                     AGLMAAELGADVKLAKRAGLLHDIGKAIDHEFDGSHVQLGAEFAKKYKENDQVIHAIE
                     AHHGDTEPKTVVACLVQAADAISAARPGARRENLESYIKRLEKLEEIATSFEGVEKSF
                     AIQAGREVRIIVNPEAVSDDKMILVARDIVKKIEDELEYPGQIKVHIIRESRAVEYAK
                     "
     gene            complement(3552..>6560)
                     /locus_tag="PLD98_07085"
     CDS             complement(3552..>6560)
                     /locus_tag="PLD98_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQA06045.1"
                     /translation="YEQAMVFPFPEKWLDSVIELYENSGEGIWHAVIRKTVVEAVNFC
                     VSLLNNAIEISSNDEKLSELYIDFLEDEIRRYKKVLDAAENGQWDELYEKVNTFSFGR
                     IPTVRGDSIFKSAAKGKRDKAKEVFKQLTEYLCATSDEHKEDMELLLPVAKELIFAAK
                     TYAAKLFEEMKAKNAYSFSVLNQLTLSLLVKEENGALVKTELAKELASGIKHVLIDEF
                     QDTNELQNTVFRALSSDGEKLFVVGDVKQSIYRFRLAMPTIFINLLETSSDYEERRFP
                     ARLFLDRNFRSRRGIVSAVNFIFSQLMSKEAGEVEYTDGHAMTAGADYDKEEKRQNPA
                     VALHILEYNKKENSDTKIESEAAYIASFIKKTIESGVLVGQKDKKRPVRASDFAILLR
                     SAKNNAAIYRNALINQGIDAYAEEKTGFFGASEISVMLSFLRAIDNPSRDVDLLALMF
                     SPIYGFTPDEIAELRSLDMKGSLYSCLKLYAAKGNIKAISLYNDIHLLRNLSGIMPTA
                     KFIRALYEKTGFMSIVGAMSHSDKRKANLSLLCEYAEEYEKNKGTGLSGFIRKMDALQ
                     RISAVLPSASAVSEGADVVRIMTIHKSKGLEFPFVIIANCAESLTNPNQKHLVTPETG
                     AGIKIKNPERMNVYKTIPSVATELLAKKNGAAEDLRLLYVAMTRAREALIMVGTVESA
                     ERKLSKTLAVTPVKGKLSPYAVNTAQSFLDLLLCGFLRHPEAAALREIAGADAIIDTL
                     PADFKLSLVINRQGSESAEEVTEECGVNEEMLREIEKRADFVYPYIPLSAVPAKMSAS
                     ELFAVRFNSDFFAQTKPSFAYGEEMSAADMGTANHKFLQLCDFKAAMKDIDSEIQRLV
                     VSGQLTEREASAVSKEDISIFLESETARNIVTADSVLRERNFTIQIPASSVFPELRDA
                     ESDEKILVQGRIDLVYIKDGRAVVVDYKTDRLNSTALLVSRYAGQLKVYLRAAAEILN
                     MPYEKVSAEIYSLHLHKSIPVEYASLFYQY"
BASE COUNT         1453 a         1663 c         1608 g         1836 t
ORIGIN      
        1 aaggccctcc tccacagcct caagggcaac ggaagggacg ggcatatatt tttcgtcaat
       61 cacatagagg gagtcgaaca tcaggggcac gaggctgata tttctcacgc cctttaaaaa
      121 gaaggggtta aggctgtcct ccaaagaaaa gggcaggcgc accctgtcaa cttgctgagc
      181 gggtcttttc gtttcgccgc cgatttttgt ttcggagctt gcgtcctccg tacccggctc
      241 ctttctgaag caggaggaga aaagaattac aaaggataaa atcagcgcga ttatttttaa
      301 ggactttttc atattccttt tcctttattc tatggtaatt gcttcgacgg atagggcttt
      361 gcccgttttt tcgtctatgg agaaaatgca ggcgttcatg ctgacctttc cggcggggtt
      421 ttcgaacctt acgggcatgc ctgttctgag cttttcgatt gcgagagaag gctcaacgcc
      481 cagaaccgag tgtacgggac ccgtcatgcc aaggtcggtg atgtacgctg tgcctttagg
      541 caaaatgcag gcgtcggagg tctgcacatg ggtatgagtg ccgaaaaccg ccgagactct
      601 gccgtcaaga tagaagccca gagccttttt ctccgccgtg gcctcagcgt ggaagtccac
      661 tataatgttt tttatgtctt taagttcatt cagaatttcg tccaccttgt caaaggggtt
      721 gcttgccgac tccatgtaaa cggtgcccgc aaggtttatt acggcgactg agaatctgcc
      781 tctgtctata atccagaagc ccttgccggg ggtggtgtgg tggaagttgg cggggcggat
      841 tacgggaacc gatgaattca gatactcgta aacctcatgc cttctgaaaa catggttgcc
      901 cgttgtgata aggtcaacgc cgctctccaa aagataattc gccgatgagg gggtaatgcc
      961 gttgccctga gcggagtttt ctccgttggc aatgcatatg tctatttgat taagtttttt
     1021 tatggccggc aagtgggcgc gaagaaactc gcatccctgt gtacctacca catcgccgat
     1081 tgccaaaata ttcatgattt gcccctttca aaccctctta taaacgaaaa cggcaagcgg
     1141 atagccgcct gccgtaaatt cactctttgc aaataaaagc acttttaagt tctgtgccgt
     1201 tattttgcat attcaacggc acggctctct ctgataatat gaaccttaat ctgtccggga
     1261 tattcaagct catcttcaat cttcttaaca atatcacggg caacaagtat catcttgtca
     1321 tccgaaactg cttcgggatt gactatgatg cgaacctctc tgcctgcctg aattgcaaag
     1381 gacttttcaa caccctcaaa ggatgtcgcg atttcctcca gcttttcaag gcgcttgatg
     1441 tagctttcaa ggttttcacg ccttgcgccg ggtcgcgcgg ctgaaatagc gtccgccgcc
     1501 tgaacaagac atgccacaac ggtcttgggc tcggtatctc cgtggtgagc ctctatagcg
     1561 tggattacct gatcgttctc cttatacttc ttggcgaact ctgcgcccaa ctgcacatgg
     1621 gagccgtcaa actcatggtc tatcgccttg cctatatcat gcaggagacc tgctctcttc
     1681 gcgagtttaa catccgcgcc cagctccgca gccataagac ctgcgatatg tgaaacctca
     1741 agggagtggt caagcacatt ctgaccgtag ctggtgcggt actttaaacg gcccaggagc
     1801 tttacgattt ccggatgtac gccgtttatg ccggcctcga taaccgctct ttcgcctgtc
     1861 tgcttaattg ttgcctcaac ctcgcggcgg gctttgtcgt acatttcctc aatgcgggcg
     1921 ggatggattc tgccgtcggc aatgagtttt tctaatgtca gccttgcaat ctctctcctt
     1981 accgggtcaa agctggagag ggtgattgcc tcgggcgtgt cgtcaatgat taggtcaacg
     2041 cctgtagtgg tctcaagggc tctgatgttc ctgccctcgc gtccgatgat tctgcccttc
     2101 atttcgtcat tgggcagagc gacaacggaa acggtagcct ctgcggcgtg gtcggcagcg
     2161 cacctttgaa tagcggtgga tataatctcc cttgccatcg cttccgcttc ttcaagggtc
     2221 ttttgcttat gctccatgag catgactgcc ttttcgtgct caagggaatc ctcaaggttt
     2281 ttaagcaggt attcctttgc ctgctcctgg ctgaatcccg aaattctctc cagcatttcg
     2341 tactggcttt tcttcagggt ttccagctct tcaagtcttt cgtcaacggt tttgagcttt
     2401 ttgtttaggg tttcgtcctt tttctcaagg gcgtccagct ttttgtcaag gctctcctct
     2461 ttttgaatca agcggcgctc ctgacgctga atttccgttc tgcgctcacg aatttcacgg
     2521 tccgcctctg tgcgcagctt atgaatttcg tcctttgctt cgataattgc ttcttttttc
     2581 ttcgtttctg cctgagcaat agcttgactg actattctgt ttgcttcgtc agtagccgag
     2641 cctattgttc tttctgctac ttttcttctg taaacgctgc cgagggcaaa tcctactcca
     2701 aagagtatga ccccgccagc gactattgcg gctatcagaa tcgggtccaa gcaatgcacc
     2761 tccttgtttt ttgtaagtcg gacgctcttt gcgcccaaat ctacggaagt gccgaaaatc
     2821 aataatattc acattttttg acatctgccc actgcgtata atgggcctat acacaaaaaa
     2881 tatagcgtca aaacagccct gcttatatta aatcagccca agctgttatc atcgattaat
     2941 cggcaatccg gaaaaatgct tgaataagtg ctgatgacac aataatgcca aatatattta
     3001 accgttattt cacagcagcg aactactgac gaaaaacata agtcaaataa aagcgcaaag
     3061 ccgcactttg aaaatatttt aactctttat cgtattaatg tcaaatgttt tatttcgcat
     3121 acgcataaaa tttagacttt ctttacaatt taagccgttt ttcgcatgtt tataccttct
     3181 caaatcgcca attccgccca cgccgcgtca gaggcgggaa cggcgctctg tcaggggtct
     3241 ccgctccctc tgttgttggg cgcaggggcg ggaatgggcc tttgccgtgg gaacgcaagc
     3301 gccgctcggg acggggttta ttttgtccgc gcggaaaccg caaagcggga gtgccgctaa
     3361 ctcgggcaat ctccgctcgg gcggattcgg ctctcgccgc tcggggcggg cgggaaaaca
     3421 cgccgcaagg aaacttcccc gccgggcggg gctcgcttga cataggcacg cggggaaaca
     3481 cctcactcaa ggcgggtatg cgctgcccca aaatcggcgg cgttttcctt gatttagagc
     3541 tattgccaat tttagtattg gtaaaaaaga cttgcatatt ccacgggaat gcttttgtga
     3601 aggtgcagag agtagatttc ggctgagact ttttcgtaag gcatattcag aatttccgcc
     3661 gcggctctga ggtaaacttt gagctgaccc gcatagcggc tgacaaggag agcggttgaa
     3721 ttcagtctgt cggtcttata atccaccacc actgccctgc cgtcctttat gtaaacaagg
     3781 tcaattctgc cttgcacgag gattttctca tcggactccg cgtcccgcag ctcggggaaa
     3841 accgaggagg cgggaatttg aatcgtgaaa ttccgctccc tgagcaccga gtcggctgta
     3901 acgatatttc tcgccgtttc gctctcaagg aaaatcgaaa tatcctcttt cgatacggcg
     3961 cttgcctccc tttcggtcag ctgacccgag acaacaagcc tttgaatttc tgagtctata
     4021 tccttcatag ccgccttaaa gtcgcaaagc tggaggaatt tgtggtttgc cgtacccata
     4081 tctgcggcgc tcatttcctc gccataggca aatgagggct ttgtctgagc aaagaaatcg
     4141 gagttaaaac gcacggcgaa aagctcggag gcggacatct ttgcgggaac ggccgaaagg
     4201 ggaatgtagg ggtagacaaa gtccgccctc ttttcaattt ctctcagcat ctcctcattg
     4261 acaccgcatt cttctgttac ttcttccgca gactctgaac cctgccggtt tattacaaga
     4321 cttagcttga agtcagcggg cagagtatcg attatggcgt cggcgccggc aatctccctt
     4381 aaagcagcgg cttcggggtg ccttaaaaat ccgcagagca gaaggtccaa aaagctctgg
     4441 gcggtgttta ccgcataggg gctgagcttt cctttaacgg gcgtgacggc aagggttttg
     4501 ctgagttttc tttcggccga ttcgacggtg ccaaccataa tcagagcctc tctggctctg
     4561 gtcatggcga catacaaaag ccttaaatcc tctgcggcgc cgttcttttt ggcaagcagc
     4621 tcggtggcga cggaggggat tgttttatag acattcattc tttcgggatt ctttatcttg
     4681 atgcccgctc ccgtttcggg ggtgacaagg tgcttttgat tgggattggt aagcgattcg
     4741 gcgcagtttg cgattatcac aaaggggaac tcaagaccct ttgacttgtg aatcgtcata
     4801 atgcgcacca catcggcgcc ctccgaaacc gccgaggcgg agggcaaaac ggcgctgatt
     4861 ctttgaaggg cgtccatctt tcttataaag cccgaaagac ccgtaccctt gtttttctcg
     4921 tactcctcgg cgtattcgca aaggagagaa agattcgcct ttcttttgtc gctgtggctc
     4981 atggctccca ctatggacat aaagcccgtc ttttcataaa gagccctgat aaactttgcg
     5041 gtgggcatta tccctgaaag gtttctgagc agatgaatat cgttatagag agatattgcc
     5101 ttaatattgc ccttggcggc gtagagtttc aggcaggagt aaagggagcc tttcatgtca
     5161 aggcttctca gctccgctat ttcatcgggg gtaaagccgt aaatgggcga gaacataagg
     5221 gcaagcaggt ccacatccct tgaggggttg tcgattgccc tcaggaatga aagcataacg
     5281 gagatttcac tcgctccgaa aaagcccgtc ttttcctccg cgtaggcgtc aatgccctga
     5341 tttataaggg cgttgcggta aattgccgcg ttgtttttcg ccgaccttaa aagtatcgcg
     5401 aagtcggaag ctcttacggg gcgtttctta tccttctgac cgaccaaaac gccgctttct
     5461 atggtctttt ttataaatga ggctatgtag gccgcctcag actctatttt ggtatccgaa
     5521 ttttcctttt tgttatattc gagaatatga agggcgactg cggggttctg acgcttttcc
     5581 tccttgtcgt agtcggcgcc cgctgtcatg gcgtgaccgt ccgtgtactc aacctcgccc
     5641 gcctctttgg acataagctg gctaaatata aagtttacag cggagactat gccccgcctg
     5701 cttctgaagt tcctgtcaag gaagagcctt gcggggaatc tcctttcctc gtagtctgac
     5761 gaggtttcga gaagatttat aaatattgtg ggcatggcga gcctgaacct gtaaatgctc
     5821 tgctttacat cgcccaccac aaacagcttt tcgccgtcgc ttgacaatgc cctgaatacg
     5881 gtgttctgaa gctcgttggt gtcctggaac tcgtcaatca gaacatgctt tattccggag
     5941 gcaagctcct ttgccagctc tgttttgacc agcgctccgt tttcttcttt tacgagaagg
     6001 gacaaagtga gctgatttaa aaccgagaag gagtaggcgt ttttggcctt catctcctca
     6061 aaaagctttg cagcataggt tttggcggcg aaaataagct cctttgcaac gggcagcagc
     6121 agctccatgt cctctttatg ctcatcggaa gtggcgcaaa ggtattcggt gagctgttta
     6181 aatacttcct ttgccttatc ccttttgccc ttggcggcgg acttgaaaat actgtcgccc
     6241 ctgactgtgg ggattctgcc gaagctgaag gtgttcactt tttcataaag ctcgtcccac
     6301 tgaccgtttt ccgcggcgtc aagcactttt ttgtaccggc ggatttcgtc ctcaagaaaa
     6361 tctatgtaaa gctccgagag cttttcgtca ttagatgaaa tctcaatggc gttattaagg
     6421 agagaaacgc agaagtttac agcctcgact acggtttttc tgataacagc gtgccagatt
     6481 ccctcgccgc tgttttcgta aagctctatt acgctgtcca gccacttttc ggggaagggg
     6541 aaaaccatgg cctgctcata
//
LOCUS       DAONVQ010000106         6381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9126_length_6381_cov_4.127411, whole genome shotgun sequence.
ACCESSION   DAONVQ010000106 DAONVQ010000000
VERSION     DAONVQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9126_length_6381_cov_4.127411"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PLD98_07090"
     CDS             complement(<1..503)
                     /locus_tag="PLD98_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA06046.1"
                     /translation="MSSWKAQLGERIKKAIGVEGSFKETIKPMLGYNSASILFGGGGY
                     IISLYFLAFLTEVEGLSPAQASAVILVSQIWDAVTDPAMGIITDRTRSRFGKHRVYLL
                     VGALPIAISYFMLWYSFGISSLGKPTATMIYYMVAYMLFNTAFTIVAVPHTAMLPELA
                     PEYFLRT"
     gene            complement(549..1031)
                     /locus_tag="PLD98_07095"
     CDS             complement(549..1031)
                     /locus_tag="PLD98_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06047.1"
                     /translation="MKKLLFYLIQFTWGLPQNLAGLIGYLILAPKHKHERFHNAIITY
                     VEKKNFGGVSLGIFIFINPGKREDWLHDTRIHEYGHTIQSLLLGPLYFFVIGIPSGIW
                     CNFPAFVKYRKEKNVSYYWLYCEGWANLWGRRWAKEDFVSEEMLTRGNFGKPLKKLSA
                     "
     gene            1432..2514
                     /locus_tag="PLD98_07100"
     CDS             1432..2514
                     /locus_tag="PLD98_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HQA06048.1"
                     /translation="MKRKVRAVQYGCGKMSVYLMRYLLEKGAEIVAAFDINPYIIGKD
                     IGEIIGCENLGVKVMDNKEADRVMAELKPDVCVVATRSTMADIKDAFTICAKNGVNAI
                     STCEEAIFPWNSSPQITKELDELAKKNNCTLAGSGYPDMYWGVLIDTLAGSMHKITRI
                     EGSSSYNVEDYGIALAEGHGAGLSIEEFNEKIGKYNDLSSDEIKAKIESGEIEPSYMW
                     NQNGWLCERLGLTPISQTQKCIPMTHTEDLESSTLGMTIKAGYATGMSAVVTTETAEG
                     ITLVTQCIGKVYAPGEFDKNEWTFYGEPDTTVIVNRPATVELTCATLVNRIPALINSE
                     PGYVTTDKLPNSPYLVKPLNEYVNEK"
     gene            complement(2573..4333)
                     /locus_tag="PLD98_07105"
     CDS             complement(2573..4333)
                     /locus_tag="PLD98_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQA06049.1"
                     /translation="MDMTMLVDTLWVLLAAVLVFFMNLGFAAVESGMARSKNTVNILS
                     KNFIVFAVSSLGFMLLGWGLMFGGDNPIVGTENLFILGNTNLDFYKDTLTSNVPFWGK
                     FFFQLVFCGTAATIVSGAVAERVKYISFIVFSFALTLVIYPIVGHWVWGGGWLGQMGF
                     LDFAGDTVVHSVGGWAALAGAILLGPRHGKYDKDGKPRAIPGHNMSLAVIGLFVLWLG
                     WFGFNPGSTMSFRNPADVMHILMTTNTAAIAAVLTATATSWIFIGKPDLGMTINGCLA
                     GLVGITGGCAYVSVTSSLIIGAIAGVIVVFAVMFFDRIKVDDPVGATSVHLVCGVFGT
                     VSVGLFAKEGVTSFSEVNGLFYGGGFSLLGKQILGVIAVGAFVFISSALVWLIIKATL
                     GIRVSLEEEIQGLDIGEHGNSAYPDFALVAPIMAAEGGAQAEVIYPAPVPAAAEANAA
                     EKVSPDEAIPVVNRARKGAKMTKVTVITNQDKFTSLQTELDKIGITGLTVTNVLGYGM
                     QKGQAEYYRGAPVKTRLLPKVQVDIVVCKIPTQTVVDTVKKALYTGNMGDGKIFIYDV
                     ENVIKIRTGEEGYDALQDEE"
     gene            4888..5499
                     /locus_tag="PLD98_07110"
     CDS             4888..5499
                     /locus_tag="PLD98_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06050.1"
                     /translation="MCESCIKFLSPEANAWCDLYCDMPNEDRENQSFDTANKDLVKQK
                     AKEVERNRKRIKEVLLSSLQLQNLMVLTGLGTSLSGGIKGPSMNELWDSIEELKVRRN
                     QILRRDEIAKKINYHGDDIEGFLSQCEAYLQVTRDKDVNNFYIKSKEVILNKCSFDFS
                     KCDLVVHKEFLFKLCRRRQRDSRLKIFTTNYDLCSKLLRVILD"
     gene            5466..5999
                     /locus_tag="PLD98_07115"
     CDS             5466..5999
                     /locus_tag="PLD98_07115"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA06051.1"
                     /translation="MFEVAASNLGLIVNAREIIANAIGRELLNMARAGYFKEKPLLVF
                     LDEAHHFLGRNIGSEDSIYRLNAFELIAKEGRKNGLNICLATQRPRDITEGVLSQIGT
                     LIVHRLINDRDREVVERACGDIDRSVASFLPNLKPGEAILVGIDFPIPLTIQIIEPST
                     PPKSDGPKYQEKWCLDN"
     gene            6177..>6381
                     /locus_tag="PLD98_07120"
     CDS             6177..>6381
                     /locus_tag="PLD98_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HQA06052.1"
                     /translation="MCGIAGWIDYNRDLRNDGDTIRRMTWTLARRGPDAEGVYLRENV
                     CLMHRRLIVIDPENGIQPITIERG"
BASE COUNT         1873 a         1426 c         1472 g         1610 t
ORIGIN      
        1 tgagtcctta ggaaatactc aggagcgagt tccggcagca ttgcggtgtg cggcactgcc
       61 actatcgtaa acgccgtatt gaaaagcatg taggcgacca tatagtaaat cattgtggct
      121 gtgggcttgc caagggagga aatgccgaag gaataccaca gcatgaaata ggatatggca
      181 atcggcagcg cgcccacaag gagatacacc ctgtgctttc caaatctcga tctggttctg
      241 tcggttataa tgcccatggc ggggtcggtt acagcgtccc aaatctgaga caccagaata
      301 accgcgcttg cctgagcggg gctaagaccc tcaacctctg ttaaaaacgc gagaaagtaa
      361 aggcttatga tatagccgcc tccgccgaaa agtatgctgg cggagttgta gccgagcatc
      421 ggcttaatcg tttccttaaa gctgccttca acgcctatgg ctttctttat tctttctccc
      481 aactgagctt tccacgaact catctgttaa cccccgaagt ttgagtttac ggttttgaac
      541 ttaaagtcct atgcgctgag ctttttaagc ggctttccga aattgccgcg ggtcagcatt
      601 tcttctgaga caaaatcctc ttttgcccag cgtctgcccc aaagatttgc ccagccctcg
      661 cagtagagcc agtagtatga aacattcttt tccttgcggt actttacaaa tgcggggaag
      721 ttgcaccaaa tgccggaggg aattccgatt acaaagaagt acaagggacc gagcagcagc
      781 gactgaattg tgtggccgta ctcatgaatc cttgtgtcat gcagccagtc ctctctcttg
      841 ccggggttga tgaaaatgaa tattcccaaa gacaccccgc cgaaattctt cttttccaca
      901 tatgtaatta tagcgttgtg gaacctttca tgcttgtgct ttggagcgag aatcagatag
      961 cctatcagcc ccgcaagatt ctgcggaagc ccccatgtga actgaattaa atagaaaagc
     1021 agttttttca tacaaaacac cccgaattta atgtaacacg cccgtttata aaagtaaata
     1081 ttattgcata tttatgtaaa tttttacttc gatgatacaa aatcgcaata tttataattt
     1141 ttctgaattt ctcccgctta gcccgcaaaa gctttgccgt tataaaaacg aatcaatatt
     1201 attccccatt ggaaagtaaa ttcttaaaag caattgcaca ataaattcta acattaaatg
     1261 acaaaatata caataaaaaa gatatagcca agctctcaca caaagtgaat aaatcggttt
     1321 tagatgctaa atctaatatt aattgccggg aaattgacaa tattttggca aaggagtata
     1381 atctgtttag cggaaaaatt acccgctaat atatcacagg gaggtaatta aatgaagcgc
     1441 aaggtaagag cagtgcagta cggatgcggc aaaatgagcg tctatcttat gaggtatctg
     1501 ctcgaaaagg gagcggagat tgtagcggcc tttgacataa acccctacat catcggcaag
     1561 gacatcggcg aaatcatcgg ctgcgagaat ctcggcgtca aggtcatgga caacaaagag
     1621 gcagaccgtg ttatggcaga gcttaaaccc gatgtctgcg tcgtcgcgac ccgcagcacc
     1681 atggcggata taaaagacgc gttcaccatc tgcgccaaaa acggcgtaaa cgctatttcc
     1741 acctgcgaag aggcgatttt cccctggaac tcctcccctc agattaccaa ggaactggac
     1801 gagcttgcaa agaagaacaa ctgcaccctg gcaggcagcg gctatcccga tatgtactgg
     1861 ggagtgctga ttgacaccct tgcaggctca atgcataaaa tcacaagaat cgaaggctca
     1921 agcagctaca atgttgagga ctacggcata gcccttgccg aaggtcacgg cgcgggactt
     1981 tctatcgagg agtttaacga aaaaatcggc aagtacaacg atttaagctc agacgaaata
     2041 aaggcaaaaa tcgaaagcgg cgagattgag cccagctata tgtggaacca gaacggctgg
     2101 ctctgcgagc gcctcggcct taccccgatt tcacagactc aaaagtgcat ccccatgacc
     2161 cataccgagg accttgagtc gagcaccctc ggcatgacaa ttaaggcagg atacgcaacg
     2221 ggaatgtccg ccgtcgtcac caccgaaaca gcggagggca tcacccttgt aacccagtgc
     2281 ataggcaagg tttacgcccc cggagagttt gacaagaacg aatggacatt ctacggcgaa
     2341 cccgacacca ccgttatagt aaaccgtccc gctacggttg agctgacatg cgccacattg
     2401 gtaaaccgta ttcccgccct tattaacagc gagccgggct atgtcaccac cgataagctg
     2461 cccaacagcc cctatcttgt aaagcccctc aatgaatatg taaacgaaaa ataaaggtta
     2521 ccgcccattt aaaggagcat attgagccgg aactcaatat gctccttttg gtttattcct
     2581 cgtcctgaag agcgtcgtag ccctcttcgc ccgttctgat ctttattaca ttttcaacat
     2641 cgtaaataaa gattttgccg tcgcccatgt tgcctgtgta aagagccttc tttacggtgt
     2701 caacaacagt ctgagtgggt atcttgcaga caacgatatc cacttgtacc ttgggcagaa
     2761 gccttgtctt aacgggcgcg ccgcggtagt actccgcctg acccttctgc atgccgtagc
     2821 ccagcacatt ggtgacggta agacccgtaa tgccgatttt gtcaagctct gtttggagtg
     2881 atgtaaactt atcctgattt gtaattacgg ttaccttagt catcttagcg cctttgcggg
     2941 cacggttgac aacgggtata gcctcatcgg gcgagacttt ttcggcagcg ttggcctcag
     3001 cagcggcggg tacgggagcg gggtaaataa cctcagcctg agcaccgcct tcagcagcca
     3061 taatcggagc cacaagggca aagtcgggat atgccgagtt gccgtgctca cctatgtcaa
     3121 gaccctgaat ttcctcttca agacttaccc taatgccgag tgtggcctta atgataagcc
     3181 atacaagggc ggaggagata aatacaaagg ctcctacagc gataacgccc agaatctgct
     3241 ttccgaggag agagaagccg ccgccgtaga aaagaccgtt gacttccgaa aagcttgtga
     3301 ctccctcttt tgcgaaaaga cctacgctaa cagtgccgaa aactccgcat acaaggtgaa
     3361 cggaggtggc gcccacgggg tcgtcaacct tgattctgtc aaagaacata accgcaaata
     3421 caacgattac gcccgcaatc gcgccgatga taagcgagct tgtgacgctg acataagcgc
     3481 agccgcccgt aatgccgaca agacccgcaa gacagccgtt gattgtcatg ccaaggtcgg
     3541 gcttgccgat gaatatccac gatgtggcgg tggcggtaag cacagcggca attgcggcgg
     3601 tgttcgtggt cataagtatg tgcattacat cggcagggtt ccggaagctc attgtggagc
     3661 cggggttaaa gccgaaccag cctagccaca gaacgaaaag tccgataacc gcaagggaca
     3721 tattgtgacc ggggattgca cggggttttc cgtccttgtc gtactttccg tgacggggtc
     3781 cgagcagtat cgcgcccgca agagcagccc agccgccgac ggagtgaacg acggtgtcgc
     3841 ccgcaaaatc aaggaagccc atctgaccga gccagccgcc gccccaaacc cagtgaccga
     3901 caatcgggta tattacgagc gtcagcgcaa aggagaatac tataaaggag atgtacttga
     3961 ccctttcagc cacggctccc gaaactatgg tagccgcggt tccgcaaaag acaagctgga
     4021 agaagaactt gccccaaaac ggcacattcg atgtaagggt gtctttgtaa aaatcaagat
     4081 tcgtgtttcc caaaataaac aggttctccg ttcccacaat gggattgtct ccgccgaaca
     4141 taagtcccca gcccaaaagc ataaagccga gggacgaaac agcgaagaca ataaagtttt
     4201 tggaaagaat atttaccgtg ttctttgacc ttgccatgcc cgactcaacc gcggcaaagc
     4261 ccaggttcat aaagaacacc agcactgcgg caagcagcac ccatagggta tccactaaca
     4321 ttgtcatgtc catactgcat tcctccttga aattttaaag gcgtgtattt cgtcttaact
     4381 atccgaaaca cacgcctttg tgcgtatatt gatttgtaat aaagtttact gaagacaaac
     4441 gctctgcgcc aaagctcatt cagctatggg ccaagtctct cctttcttta aattttttaa
     4501 ggcgtgtgtt tcagcagagt tccccgctat aaacacacgc ctttgtgcgc atttattaag
     4561 ttgtaaatga gccgccgccc gaaccgcttt gcggacgagc gcaaatctta gacacaaggc
     4621 gccgggtaat cccagcgcct ttgctctgat actatattat agtcataaag ttattatttg
     4681 tcaaccagaa ataactaaaa aatctaagac aattgtcaaa actcctacgg ttttttagta
     4741 tgtccagtag aaaacaggat attttgctat taaaggtgaa agtggtattt tattgtaata
     4801 taataaatcc gactattgct agtaaaacct atgactttga tgcttataaa ctatggcaga
     4861 aaataaacaa gataggggaa gattattgtg tgtgaaagtt gtataaagtt tctttctcca
     4921 gaagcaaacg cgtggtgcga tctttattgt gatatgccta atgaagatcg tgaaaatcaa
     4981 agttttgata cagcaaataa agatcttgta aaacaaaagg caaaagaagt tgagcgcaat
     5041 agaaaacgaa taaaggaagt attattaagt tctctccagc tgcagaattt aatggttcta
     5101 actggtttgg gtacttcttt aagtggcgga attaaaggtc cttccatgaa tgaattatgg
     5161 gatagtattg aagaactaaa agtaaggcgt aatcagattt tgcgacgaga cgaaattgct
     5221 aaaaaaatta attaccatgg tgatgacata gaagggtttc tttctcaatg tgaggcatat
     5281 ttacaggtaa caagagacaa ggatgttaat aatttttata tcaagagcaa ggaagttatt
     5341 ttaaacaaat gttcttttga tttttccaag tgcgatttgg tagtgcataa agaattcttg
     5401 tttaagcttt gccgtagaag gcaacgcgat tcaagattaa agatttttac aacaaattat
     5461 gatctatgtt cgaagttgct gcgagtaatc ttggattaat agtaaatgct cgtgaaatta
     5521 tagcaaatgc tatagggcga gaattattaa acatggctag ggcaggctat ttcaaagaga
     5581 agcctttgtt agtttttctt gatgaagcgc accatttttt aggcaggaat attggatcgg
     5641 aggattcaat ataccggtta aatgcatttg aattaatagc aaaagagggc agaaagaatg
     5701 gactgaatat ttgtttagca actcaaagac caagagatat tactgaaggg gtattgagtc
     5761 agattggaac actgattgtg catagactaa ttaatgatcg tgaccgtgaa gttgttgaga
     5821 gagcctgtgg cgatattgac cgttcagttg cttcattttt gccgaacctt aaacccggtg
     5881 aagcaatact tgttggaata gattttccta tacccctaac aattcaaatc attgagccat
     5941 caaccccacc aaaatctgat gggcctaaat atcaagaaaa gtggtgtttg gataattgat
     6001 tgattgaaaa ggtcttattt ttcattgttt tcacaatttt gtattgttaa aatgattctt
     6061 tatcgcaccc tcttttaaag cgcatataat ataactgtgt aaaataatat gagagcagat
     6121 ataatggcaa aggcgtcatt gtaatggcgc ctttttattt tttaaggagg gtttttatgt
     6181 gcggaattgc aggctggata gattacaatc gcgatttaag aaatgacggg gatacaataa
     6241 ggagaatgac atggaccctt gcccgcaggg ggcctgacgc agagggtgtg tatcttaggg
     6301 aaaatgtctg ccttatgcac cgcaggctga tagtcattga ccccgagaac ggtatccagc
     6361 ctataacaat agagaggggc t
//
LOCUS       DAONVQ010000107         6349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9181_length_6349_cov_4.643311, whole genome shotgun sequence.
ACCESSION   DAONVQ010000107 DAONVQ010000000
VERSION     DAONVQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9181_length_6349_cov_4.643311"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLD98_07125"
     CDS             <1..769
                     /locus_tag="PLD98_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HQA06053.1"
                     /translation="VTVFAGCKKQDDNGKETGTTESTAAETSPILQKDVIVVGTNAEF
                     PPFEYLLDDGVTPDGFDMALIKAIGEKMGKEVKIENMDFDAILAAIGNKVDIAIAGLT
                     VDEERLLQVDFSDTYFTAVQSIIVKENDDTIKSAEDLKGKKIGVQLGTTGDIIATDDI
                     EGATVERYKKAITAVLDLLNGRIDAVLIDSATAKALSAANKGTKVIEGDFEPEHYAIA
                     LPKGDTALKDSINKALKEVKEDGTFDELLEKYIESGE"
     gene            837..1571
                     /locus_tag="PLD98_07130"
     CDS             837..1571
                     /locus_tag="PLD98_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HQA06054.1"
                     /translation="MNLYFSTIALAMPSWMESIKQDFYISFISNDRYIVFAKGLLMTL
                     RLTFFAAIFGIIFGVLFALMKLSRTKKGKRTVFSVFASIYIDVIRGTPAVLQLLILYF
                     AVLPATASKELAAVIAFSINSSAYVAEIVRAGIQAVDHGQTEAGRSLGLSQRQTMTYI
                     VIPQAMKNILPALVNEFIVLLKETAVAGYIGVMDLTKVSDFVVSRTFIAFMPLIGTAV
                     IYFTIVKILSFFLGKLERRLRQSDSR"
     gene            1558..2280
                     /locus_tag="PLD98_07135"
     CDS             1558..2280
                     /locus_tag="PLD98_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HQA06055.1"
                     /translation="MIRVENLTKNFGSLEVLKGINYEIKKGEKIVVVGPSGSGKSTFL
                     RCLNLLEKPTSGKIWFEDQLITDPKVNIDKIRTQVGMVFQQFNLFNNLKVLDNITLAP
                     VKLKLQTKEEAQENAMRLLERVGLSDKAYAYPSQLSGGQKQRIAIVRSLAMNPKVMLF
                     DEPTSALDPEMVGEVLYVMRELAESGMTMFVVTHEMGFAREVATRVVFMDEGQIVEEK
                     TPDEFFTNPESPRLRDFLARVL"
     gene            complement(2339..3376)
                     /locus_tag="PLD98_07140"
     CDS             complement(2339..3376)
                     /locus_tag="PLD98_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06056.1"
                     /translation="MVNKDFNKIGEKHCDSLEATYSNGFWFRGPLKKYLDFNYNDSII
                     HVEINQARYRTITLTSSKIIKLSEISALLFSLEKLLMLFEGRFFTLIKLNYKGQKENE
                     KEYDLYSTESLNRRLAYYETDSCHYLFNPEFLNFYDYLSPNIIEKWLELESDLDIVHQ
                     VVLYNLSKIKLPCDVKCAYLIQSFESMVELIKKYDSELLCNLPPEKQEQIRKLQTEDS
                     RKISIINCIEAVITNFGTEIFSLELNNNQGEFFKILKNTRHRIMHIKRNQQDKHLTGD
                     QSISNQQDKYLTGEQSILYLVKISYLYRIILLKILGIDLSLYKHQLLQLVNKWNNYNG
                     ILANFLLQLNS"
     gene            complement(3403..4407)
                     /locus_tag="PLD98_07145"
     CDS             complement(3403..4407)
                     /locus_tag="PLD98_07145"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQA06057.1"
                     /translation="MSLSVMTGASGHVGYALLKELTANNVNTRILIRKDISIFKGIPC
                     EKVFGDVTDKESLVKAFEGADIVYHLAGLIDIKGGNEEMVRKINVEGTRNVVEACIQC
                     GVKRLVYMSSVDVYIPLPGNEPMTEVNRFYPDKLEGDYAKTKAEATNLVFDAEGKNGL
                     EVVVVYPGACFGPYDFKISSIGEMIRNYMDGKFPISLGFGGYNFVDVRDVAKGTYLAA
                     IKGRSGEGYLLTGTPATVDEFIRSLARAMNKKPPKIKLPLFLGNAAAPIAEAFYKLTD
                     KTPVFTRYTIRKLTSNYNFSYEKAKNELGYQPRPMDDTVKDTIKWIIANERSGKAITR
                     "
     gene            complement(4424..5908)
                     /locus_tag="PLD98_07150"
     CDS             complement(4424..5908)
                     /locus_tag="PLD98_07150"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HQA06058.1"
                     /translation="MSYASFQVFQSGKLFGLKAEKGQNLLRALRERGFVIKADCSGAY
                     TCGKCKVKIEPSPEPTEADKRLLTESEIAGGIRLACAHEVFDGITVTLPDTEEIKSAV
                     IQSVNIKAEGVLKAALDIGTTTLSLCVFNQKGEILGSVSGNNPQSSFGADVISRIKAE
                     RDFPGVQHNLLISKTNEMSESFFADLGISKPLDLFVCGNTVMLHFFFGESPVSMGEYP
                     YTPVFLESKTADGGEIGLKGFDKVCSLSCLAPFAGADITAGILSLYEETDDYKLLIDL
                     GTNAEIALFNKERCFVATAAAGPAFEGANIRCGMPSISGAVKAFKLINGNIYAETIDN
                     KELRGICGSGLISIAAELIRNRIVDKTGRMFASRFEISENVAIYPEDIRELQLSKAAV
                     RAAAEEIIERSGIGYSDISRLCLSGGFGTYINTDDAAFLGLFPPELKEKAEGIGNSAI
                     RGCVMYASDEKIRSAAERIVKSAQVFDLAVLSDFSEKLIKHIDF"
     gene            6049..>6349
                     /locus_tag="PLD98_07155"
     CDS             6049..>6349
                     /locus_tag="PLD98_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HQA06059.1"
                     /translation="MNNSPFTLHEILLKNNPPVLTFEKNKDRDLYEWREEVRKAFAEV
                     IRKPEKTLSSPAIIEYEKEHEDYHEIRFQFESEPSYFVPGHYLYPKNLTHKVPVII"
BASE COUNT         1804 a         1466 c         1307 g         1772 t
ORIGIN      
        1 tgtcacagta ttcgcaggct gcaaaaagca ggacgacaac ggtaaagaaa ccggtaccac
       61 tgagagcaca gcggcagaaa catcgcccat tctccagaag gatgttatag tagttggcac
      121 aaacgccgag ttcccgccct ttgaatatct gcttgacgac ggcgtaacac ccgacggctt
      181 cgacatggct cttattaagg ctatcggcga gaagatgggc aaggaagtaa agatcgagaa
      241 catggatttc gacgctatcc ttgcggctat cggcaacaag gttgacatcg caatcgcagg
      301 tcttaccgtt gacgaagaaa gacttcttca ggtagacttc tccgatacat atttcaccgc
      361 cgttcagagc attatagtta aggaaaacga cgacacaatc aagtccgcag aggaccttaa
      421 gggcaagaag atcggcgttc agctcggaac aacaggcgac atcatcgcaa cagatgacat
      481 cgaaggcgca acagtcgaaa gatacaagaa ggctatcacc gcggttcttg atcttttaaa
      541 cggcagaatc gacgcagtgc ttatcgacag cgcaacagct aaggcacttt ccgctgccaa
      601 caagggcaca aaggttatcg aaggcgactt tgagcccgag cactatgcaa tcgctctccc
      661 caagggagat acagcactga aagattccat caacaaggct cttaaagagg tcaaggaaga
      721 cggcacattc gatgagctcc ttgaaaagta cattgaatcc ggtgaataat cggaaaattt
      781 aaaagcactg gggcgcattc aaaaaccgag tgcgcctctt tgatttggag agtagtatga
      841 acctatattt ttccacaatt gcactggcaa tgccaagctg gatggaatca ataaagcaag
      901 acttttatat cagctttatt tcaaacgaca gatatatagt ctttgcgaaa ggcttattga
      961 tgacattgcg gctgacattc tttgctgcaa tattcggcat aatattcggc gttctttttg
     1021 cccttatgaa gctgtcacgc accaagaagg gcaagaggac cgtcttttcg gttttcgcaa
     1081 gcatttacat tgatgtcatc agaggaacgc ccgcagtatt gcagcttctc attctctatt
     1141 tcgccgttct gcccgcaaca gccagcaagg agctggcggc ggtcatcgct ttctcaataa
     1201 acagcagtgc ctatgttgcg gaaatcgtca gagcgggcat tcaggcggtt gaccacggtc
     1261 aaaccgaggc gggacgctca ctgggacttt cccaaaggca gacaatgacc tatatagtaa
     1321 taccgcaggc tatgaagaac atcctgcccg cattggtaaa cgagtttatc gtgcttttaa
     1381 aggaaaccgc ggtcgcgggc tatatcggag ttatggacct tacaaaggtc agcgactttg
     1441 tcgtaagccg aacattcata gcatttatgc cccttatcgg cacagcggta atttacttta
     1501 ccatagttaa aattctttca ttcttcctcg gcaagctgga aaggaggctg cgtcagagtg
     1561 attcgcgttg aaaatctaac caaaaacttc ggcagccttg aagttctaaa gggcatcaac
     1621 tacgaaatca aaaaaggcga aaaaatagtc gtggtaggtc cctcaggctc aggaaaaagc
     1681 actttcctgc gctgcctcaa tctccttgaa aagcctacct cgggcaagat ttggtttgag
     1741 gaccagctta tcaccgaccc caaggtaaac atcgacaaga taagaactca ggtgggaatg
     1801 gtattccagc agtttaacct ttttaacaac ttaaaggttc ttgacaatat taccttagcc
     1861 cccgtaaaac tgaaactcca aaccaaggaa gaggcgcagg aaaacgctat gcgcctgctt
     1921 gaaagggtag gactgtccga caaggcatac gcctatccct cacagctttc gggcggacaa
     1981 aagcagagaa ttgcgatagt tcgctcactt gcgatgaacc ccaaggtaat gcttttcgac
     2041 gaacccacaa gcgctcttga ccccgaaatg gtgggcgagg tactgtatgt tatgagagag
     2101 cttgccgaaa gcggaatgac aatgtttgtc gtaacccacg aaatgggctt tgcgagagag
     2161 gtcgccacaa gggtggtctt tatggacgag ggtcagattg tggaggagaa aacccccgac
     2221 gagttcttca caaaccccga aagcccaagg ctcagagact tcctcgcaag agttttataa
     2281 caacaaaggt atgcagaatt cctgcatacc tttttaattt aataatattg ttttaaactt
     2341 atgagtttaa ttgtaataag aaattagcta atatgccgtt ataattattc catttgttta
     2401 ctaattgtag caattgatgc ttgtataaag aaagatcaat cccaagtatt tttaaaagaa
     2461 tgattctata caaatatgat attttaacca aatacaggat tgattgttca ccagttaaat
     2521 atttatcttg ttggttactt attgattggt caccagttaa atgtttatct tgttggttcc
     2581 tttttatgtg cattatacga tgtcttgtat ttttaagtat tttgaaaaat tccccttgat
     2641 tattatttag ttctaaggag aatatttccg taccaaaatt tgtgataact gcttcaatac
     2701 aattaattat tgaaattttt ctgctatctt cagtttgtaa tttgcgtatt tgctcttgtt
     2761 tctcaggtgg taaattgcac aatagttcac tatcatattt ttttatcagt tcaaccattg
     2821 attcgaagct ttgaataaga taggcacact ttacatcaca gggcaattta atttttgaaa
     2881 gattatatag cactacctga tgaacaatgt ctaaatcgct ctctaattct agccattttt
     2941 caatgatatt tggtgataaa tagtcataaa agtttaaaaa ctcggggttg aataggtaat
     3001 ggcaactatc tgtttcataa tatgcaagtc ttctgtttaa gctttcagtg gaatataaat
     3061 catattcttt ctcattctct ttttgtcctt tatagtttaa tttgattagt gtaaaaaacc
     3121 taccctcaaa aagcatcaaa agtttttcga ggctaaacag caaggcagaa atttcactga
     3181 gtttgataat ttttgaagaa gttaaggtta tcgttctgta ccttgcctga ttaatctcta
     3241 catgaataat actgtcattg tagttaaagt ctaaatattt ctttaatggt ccacgaaacc
     3301 aaaacccatt tgagtatgtt gcttctaagc tatcacaatg tttttcaccg attttattaa
     3361 aatctttgtt aaccacaaaa tcccccccca gataaaatgt atttatctgg taattgcttt
     3421 gccgcttctt tcgttcgcga taatccactt aatcgtgtct tttaccgtat cgtccatcgg
     3481 gcggggctga tagcccagct cattcttagc cttttcgtat gagaagttat aattggaagt
     3541 cagtttccta attgtatacc ttgtgaaaac tggagtcttg tcggtcagct tataaaaagc
     3601 ctccgcaatc ggcgctgcgg catttccaag gaaaagcggc agttttattt tgggcggctt
     3661 tttgttcata gcccttgcga gagaccttat aaactcatca accgttgcgg gggtgccggt
     3721 cagcagataa ccttctcccg acctgccctt tatcgccgca agatatgtac cctttgccac
     3781 atctcttaca tccacaaaat tatatccgcc aaagcccaga gatatgggga actttccgtc
     3841 catgtagttt ctaatcattt cgccgatgct tgaaatcttg aaatcatatg ggccaaagca
     3901 tgcgccgggg tagacaacga ccacttcaag accgttcttg ccctccgcgt caaacacaag
     3961 atttgtagcc tctgcctttg ttttggcgta gtcgccctcc agcttgtcgg gatagaatct
     4021 gttaacctca gtcatcggct cattgcccgg caggggtatg taaacatcca ccgatgacat
     4081 atagacaagc ctttttactc cgcactgaat gcaggcttca accacatttc tggtgccctc
     4141 aacattgatt ttgcgcacca tttcctcatt gccgccttta atatcaataa gtccggcaag
     4201 atgataaacg atgtcagcgc cctcaaaggc tttaacaagc gactccttgt cggttacatc
     4261 gccgaaaacc ttttcgcagg gtatgccttt gaagatactt atgtcttttc tgattaagat
     4321 tcttgtgttt acattgttcg cggtaagttc tttaagcaat gcgtaaccca catgcccgct
     4381 ggcacccgtc ataacagata aagacatggc gattttcctt tctttaaaag tctatatgtt
     4441 taatcagctt ctctgaaaaa tcagacaaaa ccgcaaggtc aaagacctga gcagacttga
     4501 caatcctttc tgcggcagac cttatttttt catccgaggc atacatgacg catcctctaa
     4561 tcgcagaatt gccgattcct tctgcctttt ctttcagctc aggcgggaaa agccccaaga
     4621 atgcagcgtc atccgtattt atatatgttc cgaagccacc cgaaagacag agtcttgaaa
     4681 tatcggaata tccgataccc gacctttcga ttatctcctc agcggcagcc cttacggcag
     4741 ccttgctgag ctgcagttct ctgatatcct ctgggtatat agccacattc tcgcttatct
     4801 caaagcggct tgcaaacatc cgccccgtct tgtcaacaat tctgtttctt attagctccg
     4861 cggcaatgct tataagcccc gagccgcaaa tgccccttag ctctttattg tcaatggttt
     4921 cggcatagat gttgccgttg attagcttaa aagccttcac agcgcccgag attgacggca
     4981 tgccgcacct tatgttagcc ccctcaaaag cgggaccagc ggctgcggtc gccacaaagc
     5041 agcgctcttt attaaaaagg gcaatctccg cgttcgtgcc aaggtcaata agcaatttgt
     5101 aatcgtcggt ttcttcatac agactcagaa ttcccgccgt aatgtccgcc ccggcaaagg
     5161 gcgcaaggca ggagagggag cagaccttgt caaagccttt aagcccgatt tcgccgccat
     5221 ctgctgtttt tgactcaaga aaaacagggg tataaggata ttcacccatc gaaacgggac
     5281 tttctccaaa gaagaagtgg agcatgaccg tatttccgca gacaaataaa tccaagggct
     5341 tcgatatgcc aaggtccgca aagaaacttt cgctcatttc gttagtttta gaaatcagca
     5401 agttatgctg aacgccgggg aaatcccttt cagcctttat cctcgaaata acatccgccc
     5461 caaaggagct ttgagggttg tttcccgaaa cgctgcccaa aatctcgccc ttttgattaa
     5521 atacacaaag ggagagggta gtggttccta tatcaagcgc agcctttaaa acaccctcag
     5581 cctttatatt aacactctga ataaccgcag actttatttc ttctgtatcg ggcagggtaa
     5641 ctgtaatacc gtcaaaaacc tcatgagcgc aggcaagtct tatgccgccc gcaatttcag
     5701 actctgttaa gagcctttta tccgcttctg tcggctcagg tgacggctca atctttacct
     5761 tgcatttccc gcaggtatat gcgcccgagc agtctgcttt aatcacaaag cctctttctc
     5821 taagagctcg gagcagattt tgcccctttt cagcttttaa gccaaagagc ttcccgcttt
     5881 ggaaaacctg aaaagaagca taactcatac cctgcctcca agatttgtaa actgttgata
     5941 ttttaacata taggcataaa aggtgcaata atgacttgag tttattgcat tgacataaaa
     6001 taggtggtat tattttctta taacagctaa accgaaagaa gggtaataat gaacaacagt
     6061 cctttcacat tacatgagat tttgctcaag aacaatcccc ccgtccttac ctttgagaaa
     6121 aataaggaca gggatttata tgagtggaga gaggaagtaa ggaaagcctt cgccgaggtt
     6181 ataagaaagc ccgagaaaac cctttcttct cccgccataa tcgagtatga aaaggagcac
     6241 gaagattatc atgaaattag atttcagttt gaaagtgagc catcatattt cgtgcccggt
     6301 cactaccttt atcccaagaa cttaacccac aaggtccccg tcatcatct
//
LOCUS       DAONVQ010000108         6347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9186_length_6347_cov_5.415130, whole genome shotgun sequence.
ACCESSION   DAONVQ010000108 DAONVQ010000000
VERSION     DAONVQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9186_length_6347_cov_5.415130"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="dcm"
                     /locus_tag="PLD98_07160"
     CDS             complement(<1..659)
                     /gene="dcm"
                     /locus_tag="PLD98_07160"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR00675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HQA06060.1"
                     /translation="MKKYNIMDLFAGVGGLSYGFSKIPEFNIVAANEIEKDIAAAYFL
                     NHPNVTMLNCDICSLTDHMLHDALGGKKIDIVVGGPPCQSYSTLGKRQMDDRANLFIQ
                     YKRVLGILRPKAFIYENVVGLLSMNQGRLFNTVLSEFSELGYELKYKVLNAVNYGVPQ
                     FRERVILVGFLGENKYEFPEPTHGEGLKPYVTLKDALGDLPPLSSGETNNTYLHEPDN
                     D"
     gene            complement(834..908)
                     /locus_tag="PLD98_07165"
     tRNA            complement(834..908)
                     /locus_tag="PLD98_07165"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(874..876),aa:Cys,seq:gca)
     gene            complement(957..1032)
                     /locus_tag="PLD98_07170"
     tRNA            complement(957..1032)
                     /locus_tag="PLD98_07170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(997..999),aa:Gly,seq:gcc)
     gene            complement(1072..1147)
                     /locus_tag="PLD98_07175"
     tRNA            complement(1072..1147)
                     /locus_tag="PLD98_07175"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1112..1114),aa:Phe,seq:gaa)
     gene            complement(1152..1228)
                     /locus_tag="PLD98_07180"
     tRNA            complement(1152..1228)
                     /locus_tag="PLD98_07180"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1192..1194),aa:Asp,seq:gtc)
     gene            complement(1235..1310)
                     /locus_tag="PLD98_07185"
     tRNA            complement(1235..1310)
                     /locus_tag="PLD98_07185"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1275..1277),aa:Val,seq:tac)
     gene            complement(1567..2439)
                     /gene="hslO"
                     /locus_tag="PLD98_07190"
     CDS             complement(1567..2439)
                     /gene="hslO"
                     /locus_tag="PLD98_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HQA06061.1"
                     /translation="MAHSVRCISKDGTLFIMAADTSDIVETARLIHSTSKVCSAALGR
                     LLTAGVFMGQMLKGESDTLTLRIKGGGPAGVVVAVADSKGNVKGYIENPNVELPLNEK
                     GKLDVGKAVGKDGFLTVMRDLGLKEPYIGQVPLVSGEIAEDITAYYAMSEQTPTVCAL
                     GVLVNPDKTIAVAGGFIVQLLPTADDKIIEKVEKSLEDIPPVTKMLAEGMTPVDICKR
                     VLSEFELELLDETNPEYRCGCSKDRVVRALISLGKEELLSLAEDEVTEVNCQFCNKKY
                     KFTPGEIKALVKEV"
     gene            complement(2527..3270)
                     /locus_tag="PLD98_07195"
     CDS             complement(2527..3270)
                     /locus_tag="PLD98_07195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQA06062.1"
                     /translation="MSAYDALARFYDLLTDDVEYERRGEYIHSLLKEYGAEGGIMLDL
                     SCGTGTLTEFFWKKGYDMIGVDKSPEMLMKARQKFFFSGCDALLLCQDMRELDLYGTV
                     DCAICTLDSINHLPTIEDVSLAFSRVSLFMNKGGVFVFDVNTEYKHREILGDNTFVME
                     EDDVFVVWQNFYEESDKSTDIRLDIFTKNGELYERSTEDFTEWAYSTAQLKAALENNG
                     FEVKAVYDDLTRKPERADSERLYFAAVKI"
     gene            complement(3267..3563)
                     /locus_tag="PLD98_07200"
     CDS             complement(3267..3563)
                     /locus_tag="PLD98_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HQA06063.1"
                     /translation="MIDKIRDDGTDLLFSAILTLETLDECYRFFDDLCTVPEIRAMAQ
                     RIVVAKMLRENKHYSEIAESTKVSTATISRVKRSLHYGRDGYDIVFKRLEKQQK"
     gene            3884..4237
                     /locus_tag="PLD98_07205"
     CDS             3884..4237
                     /locus_tag="PLD98_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06064.1"
                     /translation="MKRKVRNRALSIVLALLVLLSSVSLSLPAAAAFVRAENYNSVDK
                     AVLTPEQAATLILDYADKILAENGTVMTTSVPFFGSIVVDTTSIDKTLKSIDDLSVFI
                     VAASKIPILGLLSAL"
     gene            4234..>6347
                     /locus_tag="PLD98_07210"
     CDS             4234..>6347
                     /locus_tag="PLD98_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06065.1"
                     /translation="MIGDLDDLNFNAIKGKRRGSGNADLQLIKSLIQFAFDNRDFITK
                     AVQGNIDLGIINSFVDVNEIIGDISEMIRGLIYDSLVKRDEYPLTYEQAKNDARFNTA
                     DALINTFITDLLTKPTSFDASSVDTGAILSTLSSFLSFLGLPVDLTGLSIADITKMIT
                     ELMGLPGAEAVPALSPGAVIMPSLERSKIDLTRNSLYSVIEYVVNTAYTDLLALPLNN
                     GLKQLLAVLSGAQLNEVVNTSLLSNLSKNAPKKSNAISNTDFFTYSGKQYVRTGDKYF
                     EVNWDNSNDFYSVINWNYAFPNNLSGLGADTDFAKNANKLLYRATQTVFANSAFSSMG
                     LVDGGNEHLNENINKLLKFILPKIPPETFGANFDTSLLENIENKSLEQLAALLLRGIL
                     QTNLPSLIIPSSVKTLEEMAILALREVLIYEAPHLKYDDRIFSNAAALELKNKTESEW
                     KELLFEMIVDAAVFYLDQYAGLDFGVDKVLAKKNRGWTAVDFFDEIVDWALSYTKGIL
                     LVTNELNLTRGTTQNPWEKLNKVIESLVDLSFINNAGGYQNDKYYVLDTETFVKYKIL
                     GNLLNLNAADAVNMFVRNAEDGNILNDPNGMRGILKTVNKAIKAVLNTELSAGFLTSA
                     ESLIKKEGLVELVNKLIPALNRRANASVNNLLDAVLPFVASALPDVLTEQRFGGLKID
                     APDYIVISSGSAKGTVKLTHST"
BASE COUNT         1638 a         1547 c         1421 g         1741 t
ORIGIN      
        1 aaatcgttgt caggctcgtg cagatatgtg ttgtttgtct cgccgctgga aagcggaggg
       61 aggtcgccca gggcgtcttt taaggttaca taaggtttta agccttcacc gtgggtgggt
      121 tcgggaaatt cgtatttgtt ctctcccagg aagccgacca gtattaccct ttctcgaaac
      181 tggggcacgc cgtaatttac cgcgttcaat accttgtatt taagctcata gcccagctcg
      241 ctaaactcgg aaagcacagt attaaaaagc cttccttggt tcatgctgag aagccccact
      301 acattttcgt atataaatgc cttgggtctt agtatcccca aaactctttt atattgaata
      361 aacaggttgg ctctgtcgtc catctgcctt ttaccgagag tagaataaga ctgacagggc
      421 ggaccgccga ccacaatgtc tatttttttg ccgccgaggg cgtcgtgtaa catatgatcc
      481 gtaaggctgc atatgtcgca gttaagcatt gtgacattgg gatgattgag aaaatacgcg
      541 gcggcgatgt ccttttctat ctcattggct gcgactatat tgaattcggg tatctttgaa
      601 aaaccgtagc ttaggccgcc gacccctgca aacaagtcca ttatattgta ttttttcacc
      661 ggaactctcc tttttgattt agtccaaagc tcagcaactc tgcaaaataa cccgatgttt
      721 ctcctttgcc tatggctatt ttagtctaag tttataccat agtcaaatca ataagagggt
      781 ttttgggcaa aataaaaacc tcggctttga aaatcaaagc tgaggctaat ttttggaggc
      841 gccacccaga tttgaactgg ggagtcaggg ttttgcagac ccgtgcctta ccacttggct
      901 atggcgcctt aacaaaaaac tcgtactgag agttttcaac atcagcacga gtttagtgga
      961 gcggatgacg aggctcgaac tcgctacctc caccttggca aggtggcgct ctaccagatg
     1021 agctacatcc gcacggcata gagattaatc tctatagcca atcacattta gtggtgcctt
     1081 tggtcggaat cgaaccaacg acacgtggat tttcagtcca ctgctctacc aactgagcta
     1141 caaaggcaag gtggcgaccc ggaacgggct cgaaccgtcg acctctagcg tgacaggcta
     1201 gcgttctaac caactgaact accgggccac ttcctggtgg gagcaacagg gctcgaacct
     1261 gtgacccctt gcttgtaagg caagtgctct cccagctgag ctatgctccc gcgcactgcc
     1321 tttagactat actacaaaac gcctttgttg tcaacacatt tttttaaatt tgtttcgcgt
     1381 ccaaaatgtg ccgattgagc gggaccgtaa gcctaatctt tggcatatgt gccggcttta
     1441 agcgattggc ttggcaaatg ctgcggaaag aattatagca taattccttt atgctgacaa
     1501 tggggttttt aaaatttttt agagtaaaat ttgctaataa gcagtatatg ccaaatagcc
     1561 tcaaaattac acttctttta caagcgcctt tatttcgccg ggggtgaact tatacttctt
     1621 attgcaaaat tggcagttta cctcggtgac ttcatcctcg gcaagactta aaagctcctc
     1681 tttgcctaaa gaaatcaagg ctcttaccac cctatccttg ctgcagccgc agcggtattc
     1741 ggggtttgtt tcgtccaaaa gctcaagctc gaactcggac agaactcttt tgcaaatatc
     1801 caccggggtc atgccctccg ccagcatttt tgtgacgggg ggaatgtcct ctaaggattt
     1861 ttctaccttt tcgattattt tgtcatcggc ggtggggaga agctgcacga tgaagccgcc
     1921 ggcgacggct attgtcttat cgggatttac aagcacgccc aatgcgcaga ctgtgggggt
     1981 ctgctcgctc attgcgtaat aggctgtgat gtcctcggcg atttcgccgg ataccagcgg
     2041 aacctgaccg atatatggct ctttaagacc caaatcgcgc atgacggtca aaaagccgtc
     2101 cttgccaaca gctttgccca catccagctt gcccttttca ttcaaaggca gctccacatt
     2161 aggattttcg atatagcctt ttacatttcc tttgctgtcg gcgaccgcta cgacaacgcc
     2221 cgcgggtccc ccgcccttaa ttctcagggt gagggtgtcc gattcgcctt ttagcatctg
     2281 acccataaaa actccagccg taaggagtct gcccaaagcg gctgagcaaa ccttggaggt
     2341 ggagtgaatg agtcttgccg tttccacaat gtcgcttgtg tcggccgcca ttataaaaag
     2401 cgtgccgtcc tttgaaatgc atctgactga atgagccata gagtttctcc ccaaatttat
     2461 gcccgaaact taaaagcttc gggcattttt atttattata acattttttc tctgatttta
     2521 aaaagcctat atcttcacag cggcaaaata gagcctctcg ctgtcggctc tttcgggctt
     2581 tcttgtaagg tcgtcgtaaa cggctttcac ctcaaagccg ttattttcga gagccgcttt
     2641 cagctgggcg gtgctgtatg cccactcggt gaaatcctcg gtgcttcttt cgtacagctc
     2701 gccgtttttt gtgaaaatgt caaggcggat gtcagtgctt ttgtcgcttt cctcatagaa
     2761 attctgccag acgacaaaaa catcatcttc ttccatcaca aaggtgttgt cccccaaaat
     2821 ctccctgtgc ttatactcgg tgtttacatc aaaaacgaaa accccgcctt tgttcataaa
     2881 gagggagacc cttgaaaaag caagggacac atcttcgatt gtgggcaggt ggttaatgct
     2941 gtcaagagtg cagatagcgc agtcaacggt gccgtagagg tccagctccc gcatatcctg
     3001 acacagaagc agcgcgtcgc agccgctgaa aaaaaacttc tgccttgcct tcatgagcat
     3061 ttcgggggac ttgtccacgc ctatcatgtc gtagcccttt ttccaaaaga actcggttaa
     3121 agtacccgtg ccgcatgaaa ggtcaagcat tatgccgccc tctgccccgt attccttcag
     3181 caaagagtga atatattcgc cccgcctttc atattccaca tcgtcggtaa gtaaatcgta
     3241 aaagcgggca agagcatcgt aggcactcat ttctgctgct tttccagcct tttgaagact
     3301 atgtcatatc cgtcgcggcc gtaatgaagg ctgcgcttta ctctgcttat ggtagcggtg
     3361 gaaacctttg tagactccgc aatctcgctg taatgcttgt tttctctgag cattttggca
     3421 acgactattc tctgagccat tgcgcggatt tcgggcactg tgcaaaggtc gtcaaagaac
     3481 ctgtagcact cgtcaagggt ttcaagagtc aaaatcgcgc tgaaaagcag gtcggttcca
     3541 tcgtccctta tcttgtctat cacagcaaat cctccttaaa gaaagactat taagtcttgt
     3601 gattttcaga gctttttgcc agtaactaaa tttgttttaa gtttagcata ttaaactgta
     3661 aaagtaaaca acgaaagttt aaaaaaatat aaaattttca tcaaaagtca ctttgtccct
     3721 gccaagattt acagcggtca aagaggcgat tgaaggctta tttgtcgggc atttttattg
     3781 acatggaggg ctttttattc tatccttaag ataaggtatt tttctattat tgaatattcc
     3841 ggaattaagg gaatcggatt tacataaggg gaagtggaag agtatgaaaa ggaaagtcag
     3901 aaaccgcgct ttaagcatag ttctcgctct gctggtgctt ttaagctcgg tatctttaag
     3961 cctgcccgcc gcggctgcgt ttgtacgggc tgagaactac aactccgtgg acaaggctgt
     4021 gctcactccc gagcaggcgg ccacgctgat tttggattac gcggataaaa tccttgccga
     4081 aaacggcacc gttatgacca caagcgttcc ttttttcggc tcaattgttg tggacacaac
     4141 atcaattgac aaaacattaa aatcaattga tgatttaagc gttttcattg tcgcggcctc
     4201 taaaatacct atcctcggcc ttttatcggc gctttgatcg gagaccttga tgaccttaat
     4261 ttcaacgcca tcaaaggcaa acgcagaggc tccggcaacg ctgatttgca gcttataaag
     4321 agcttaatcc aatttgcctt tgataaccgt gattttatta caaaagcggt tcagggcaat
     4381 attgatttag gcataataaa tagctttgtg gatgtaaacg agattatcgg cgatatttcc
     4441 gagatgataa ggggtcttat atatgactcc cttgtaaaga gggatgaata tcctctgact
     4501 tatgaacagg caaagaacga cgcgagattc aataccgccg acgcgctgat caatacattt
     4561 atcaccgacc ttttgacaaa acctacaagc tttgacgctt caagcgtgga tacgggggca
     4621 attctcagca ccctgtcatc cttcctttca ttcctcggtc tccccgttga ccttacaggt
     4681 ctttctattg ccgatataac aaaaatgatt acggagctta tggggcttcc cggcgcggag
     4741 gctgtacccg ctctttctcc cggcgcggta attatgccgt cccttgagcg ctccaaaata
     4801 gaccttacaa gaaattcgct ttacagcgtc attgaatatg tagtaaacac tgcttacacc
     4861 gaccttctcg cgctgcccct caataacggc ttaaagcagc ttctcgcagt gctcagcggc
     4921 gctcagctta atgaagtggt aaacacaagc cttctttcaa acctttcaaa aaacgcgcct
     4981 aaaaagtcaa acgcgatttc aaataccgat ttcttcacat actccggcaa gcagtatgtc
     5041 cgcacaggcg acaaatactt tgaggtaaat tgggataact cgaacgattt ctacagcgtt
     5101 ataaactgga attatgcctt cccgaataat ctctcgggac ttggcgctga taccgatttt
     5161 gccaaaaacg cgaacaagct gctttacagg gcgactcaga ctgtatttgc caactccgcc
     5221 tttagctcaa tgggtcttgt tgacggcgga aatgagcatc tgaatgaaaa cataaacaag
     5281 ctgcttaaat tcatactgcc gaaaatcccg cccgagacat tcggcgctaa tttcgacact
     5341 tccctgcttg agaatataga aaacaaaagc cttgagcagc ttgccgccct gcttttaagg
     5401 ggcatactgc agactaacct gccctccctt attatcccct cctcagtgaa aacactggag
     5461 gagatggcga ttctcgcact cagagaagtg ctgatttacg aggctccgca tcttaaatac
     5521 gatgacagga ttttcagcaa tgcggccgca ttggagctga aaaacaaaac tgagagcgag
     5581 tggaaagagc tgctctttga gatgatagtt gacgcggctg ttttctatct tgaccagtac
     5641 gcgggtctgg atttcggcgt tgacaaggtt ctcgctaaga aaaatcgggg atggaccgcc
     5701 gtagacttct ttgacgaaat tgttgactgg gctctttcct atacaaaggg catacttttg
     5761 gtgacaaatg agcttaacct cacaaggggc acgacccaga acccgtggga gaagctcaac
     5821 aaggtcattg agtcccttgt tgacttaagt ttcataaaca acgctggcgg ttatcagaac
     5881 gacaaatact atgttctgga caccgaaacc tttgttaagt ataagatttt gggaaatctt
     5941 ttaaacctta atgccgctga cgctgtgaat atgtttgttc gcaacgcaga ggacggcaat
     6001 atcctcaatg accccaacgg catgagggga attctgaaaa cggtaaacaa ggcaataaaa
     6061 gcggtactta atacagagct ttccgccgga ttccttactt ccgcagaatc gcttatcaaa
     6121 aaggaaggac ttgtggagct tgtcaacaag ctcattcccg ctcttaaccg ccgcgcgaat
     6181 gcgtccgtca acaatctgct tgacgctgtt ctgccttttg tcgcctcggc gctccccgat
     6241 gttctgacag agcagaggtt cggcggactc aaaattgacg ctcccgacta tattgtcatc
     6301 agctccggca gcgcaaaggg aacggtaaag ctgactcaca gcaccat
//
LOCUS       DAONVQ010000109         6343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9194_length_6343_cov_5.139472, whole genome shotgun sequence.
ACCESSION   DAONVQ010000109 DAONVQ010000000
VERSION     DAONVQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9194_length_6343_cov_5.139472"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PLD98_07215"
     CDS             <1..786
                     /locus_tag="PLD98_07215"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQA06066.1"
                     /translation="RFACFWMCVVIYINKKGRAEKRERRLYNIINSLAVLMLIAAALL
                     IVWTALIRIDKLREQYLHFQLFLEDLQLFVISLKSKWLIIIAIFLLYIIDAYTGILPM
                     NFLIVLSGIVFPMPAAVLINIAGLLILMSIKYDMGSKKGAGSAKHILKRYPGVMDFLE
                     DDKKSGLLLVVFRLVPNFPFGMVSRLYGSAHFPFEKFIFLSLLGYVPRLLSYSFIGNK
                     VFDPFSAAFFTPIIILLIISGFSMLVLNAIVFGVNKTQPDERK"
     gene            791..2074
                     /locus_tag="PLD98_07220"
     CDS             791..2074
                     /locus_tag="PLD98_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HQA06067.1"
                     /translation="MSNVKTVLESLNNGGFDRVFATLYGESALPRQKKRYADIVNVFE
                     DLYKDGEIRFFSAPGRTEVCGNHTDHNRGRVLAAAVNLDVIAAASENDENIIRLKSVE
                     YPKMDVIDLNDLEPKAEEAGKSAALIRGVASRLKQLGYNIGGFNAVTTTDVFSGSGIS
                     SSAAFEVLVGTILNHLYNDGKISPVEIAKTAQFAENKFFLKPCGLMDQMACSVGGFVG
                     IDFKDTENPIIEKVSLDFNEFSHALCIIDTGGDHSDLTDEYAAIRKEMESVAAYFGKS
                     ALRDVNEDDFYNNLDDIRKKTSERAVLRAMHFFNENKRVEKALESLKSGDFNEFKSVI
                     TNSGLSSYMYNQNVYSNKNPLSQPVSLALALCEKLLYGKGAYRVHGGGFAGTVQAFVP
                     NELLSDFKSEIEKVFGENRCFVLKIREQGGTAVNP"
     gene            2126..2884
                     /locus_tag="PLD98_07225"
     CDS             2126..2884
                     /locus_tag="PLD98_07225"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HQA06068.1"
                     /translation="MGKDTAKKRLHLLDYFRGIAFVNMAIYHALFLLDDMYNIRPLPD
                     LIMEIYQNLICSGFILISGAALSLMKKSGRDILKLVLAAAAVSVATYIATPEYFISFG
                     ILHFFSLALIIGTAVKPLLYKIPPLFGAALNLLLFTLFYAADDGYGGIFTLRLFDFPD
                     ALYNFPLSFVFGFPSPTYTSGDYFGLIPWIFLYFAGFFIFRIIRENKKALDFFYKNPK
                     PYFLSFFGRNSLIFYLIHQPIIYAVLYVIFKIIR"
     gene            complement(2885..4231)
                     /locus_tag="PLD98_07230"
     CDS             complement(2885..4231)
                     /locus_tag="PLD98_07230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HQA06069.1"
                     /translation="MRKFLISALVCLIILTTSVNALASYNSEIDVEAEIVFLQSMDDG
                     TVIFDKNADMLTAPASLAKIVTASIVLENAQNLDEIVEVPNYAIRLLDGTNSSTAGLL
                     PGEQMSVRNLLYCMLVASANDAANVLADYICPNNIGAFVQMMNEYVKSIGCENTVFIN
                     PSGLDAEGQYTTARDVAKFTLKALENPIFEEITSTKKYTIPPTNKYPQERNLISTVKI
                     MNSGIKDYYIPTAKGIKTGTTSNAGRCIVTKASKDGYTYLAVVMRAPFYDIDNDGVEE
                     NIAFMECKKILDWTFKNIKLRLVAEKNQVVSVIDVGLSSKYDHLRLLPEKEITALVPS
                     GVNAQGVYFEVIEELTPKKVDAPIKKGQKIGRARILYAGNEIAQVDLVAGETIERNFL
                     LYLAKITKTVLTSPVFIILAAILAAAVVVILILRAKNSRRKTVIRVVKDYSALKRR"
     gene            4326..5015
                     /locus_tag="PLD98_07235"
     CDS             4326..5015
                     /locus_tag="PLD98_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009985826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA06070.1"
                     /translation="MIYITGDTHGDESRLSTRRLKKLGEGDTLIICGDFGFIWDGSKR
                     EQKFLAKLGSRKYNICFIDGAHERFELLNKYPVVQWNKGWARQISGNLMYLMRGQIYE
                     IEGKKIFTMGGGETLEFDLRDDSGLWTRAELPSKEELLEGIENIERVGFEVDLVITHE
                     PPAKVKEFLRLRDSEPVRVSAFNAYLQEFSTLCRYKKWYFGSMHIDKYISASQTAVFK
                     DIVEAFSGEKI"
     gene            complement(5017..6141)
                     /locus_tag="PLD98_07240"
     CDS             complement(5017..6141)
                     /locus_tag="PLD98_07240"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQA06071.1"
                     /translation="MNISSYMPVDVFYGKNALLENGKELKKLGSRCLIVTSPTAAKKS
                     GALDDAVKVLSQEGIEYEIFDRIEENPLTSTCHSAGTAAYEFKADFILGIGGGSPLDA
                     SKAIAVFASNRHMEPDDIYTAEIKNTPLPAALVGTTAGTGSEVTAVSVLTNSKTGFKK
                     GVGGKAYYAKVAFCDPSYTSSMPYSVTVSTALDAMAHALESILCLDANDMSDLYAYKA
                     VELLTEGLQRLDKIQSAEEINEDTRDLLYTASLIAGYSLNITGTCFPHTVGYILTERF
                     NIPHGRACAAFTPEFLERGRKHMPEKTEKVLKAANMNFDDFVALIKRLAAIPELRLSQ
                     EEIDKIALRWNDSIKNFCRTPGGYTAADAKSSLISVVKPL"
BASE COUNT         1627 a         1514 c         1470 g         1732 t
ORIGIN      
        1 aggtttgcct gtttttggat gtgtgtggtg atttatataa acaaaaaagg gcgcgctgaa
       61 aaacgcgaaa ggcggctata caatataatc aactccttag cggttttgat gctcatagcc
      121 gcggcgcttc ttattgtttg gaccgccctt attagaatag ataaactaag ggagcagtat
      181 ctgcattttc agctctttct tgaggatttg cagctctttg tcatctccct taaaagcaaa
      241 tggcttatta tcatcgccat tttccttctt tatataatcg acgcttatac gggcattttg
      301 cccatgaact ttctgatagt cttgtcaggt attgtattcc ccatgcccgc cgcggtgctt
      361 ataaacatag cgggactgct gattcttatg agcataaagt acgatatggg ctctaaaaag
      421 ggagcgggca gcgcgaaaca tattcttaaa agatacccgg gtgtaatgga ctttttggag
      481 gacgacaaaa aaagcggatt attgcttgtc gtattccgac tcgtgcccaa cttccccttc
      541 ggcatggtaa gccgtcttta cggcagcgcc catttcccct ttgaaaagtt tatcttcctc
      601 tcccttttgg gctatgttcc gagacttctg tcatacagct ttatcggtaa caaggtcttt
      661 gaccccttct ccgcggcgtt cttcacaccg attataatcc tgcttattat ctcaggcttc
      721 tcaatgcttg ttctgaacgc tattgtcttt ggagtaaaca aaacccaacc cgatgaaagg
      781 aaatgagaaa atgtcgaatg ttaaaaccgt tttagaaagt ctgaacaacg gcggctttga
      841 cagagttttt gcgactcttt acggtgaaag cgcgctccct cgccaaaaaa agagatacgc
      901 ggacattgta aatgtctttg aggatttata caaagacggg gaaatcagat tcttctcagc
      961 ccccggcaga acggaggtct gcggcaacca taccgaccac aacagaggca gagtgcttgc
     1021 cgccgcggta aaccttgatg ttatcgccgc cgcctccgaa aatgacgaga atatcatcag
     1081 gcttaaatcc gtagagtacc cgaaaatgga tgttatagat ttaaatgatt tagagcccaa
     1141 agcagaggag gcgggcaaat ccgccgccct tataagaggc gtcgcttcaa ggctcaaaca
     1201 gctcggatac aacataggcg gttttaacgc cgttacaacc accgatgttt tctcaggttc
     1261 gggcatttcc tcctccgcag cctttgaggt acttgtggga actatcctca accaccttta
     1321 caacgacggc aaaatctctc ccgtagaaat agccaagacc gcccagtttg cggagaataa
     1381 gtttttcctt aaaccctgcg gacttatgga ccagatggct tgctccgtcg gcggttttgt
     1441 gggcattgac tttaaggaca ccgaaaaccc gattatcgaa aaggtaagcc ttgattttaa
     1501 tgagttctcc cacgcccttt gcattataga tacgggcggt gaccattcgg atttaaccga
     1561 tgagtatgcg gcaatccgca aagaaatgga aagcgtcgct gcgtatttcg gaaagtccgc
     1621 tttaagagat gtaaacgaag acgatttcta caataatctc gacgatatta gaaaaaagac
     1681 aagcgagcgg gcggttctca gagccatgca cttctttaac gaaaacaaga gggtagagaa
     1741 ggctcttgag tctttaaaga gcggcgattt taacgagttt aagtccgtta tcacaaacag
     1801 cggtctttct tcatatatgt ataatcaaaa tgtctactcg aacaaaaacc cgctttcaca
     1861 gcccgtttcc cttgcccttg ccctttgcga aaaactcctt tacggtaaag gagcttacag
     1921 agttcacggc ggcggctttg cgggcaccgt tcaggcattt gtgccgaatg agcttttaag
     1981 cgattttaaa tcggaaatcg aaaaggtatt cggcgagaac cgctgctttg tcttgaaaat
     2041 cagagagcag ggcggcacag cggtaaatcc ttaaattata tgagtgcggc ttatgccgcg
     2101 ctcttttctt aaatcggagg cgcttatggg aaaggatacg gcaaaaaaaa gacttcacct
     2161 tcttgactac ttcagaggca tcgcctttgt gaacatggcg atatatcacg ctctgtttct
     2221 ccttgatgat atgtataata tccgccccct gcccgaccta ataatggaga tataccaaaa
     2281 ccttatttgc tcaggcttta tactcatctc gggagctgcc ctgagcctta tgaaaaaaag
     2341 cggcagggat atattaaaac ttgtactcgc ggcggcggct gtaagcgtcg ctacatatat
     2401 agccacgccg gaatacttta tttctttcgg catactgcac tttttctccc ttgcgcttat
     2461 aatcggcacc gccgtaaaac cgcttcttta taaaataccg ccccttttcg gagcggcttt
     2521 aaatcttctg ctgtttaccc ttttctatgc cgctgacgac ggctacggcg gcattttcac
     2581 cttaaggctg ttcgatttcc ccgatgcgct ttataatttc ccgctttcct tcgttttcgg
     2641 tttcccttct cccacctata catctggtga ctatttcggt cttatccctt ggattttcct
     2701 ttacttcgcg ggcttcttta tattcagaat tatcagggaa aacaaaaagg ccttggattt
     2761 cttttataaa aacccaaagc cgtacttttt aagttttttc ggcaggaact ctctgatttt
     2821 ctaccttatc caccaaccga ttatttacgc tgttctctat gtgattttca aaattatccg
     2881 ctgattatct ccttttcagc gcgctgtagt ctttcacaac ccttatgaca gtctttcttc
     2941 tgctgttctt tgccctgaga atgagaatca caaccacagc ggcggccaga attgccgcaa
     3001 ggataataaa tacgggagag gtcagcacag tttttgtgat tttggcaagg tataaaagga
     3061 agttcctctc aatcgtttct cccgctacaa ggtcaacctg agcgatttcg ttgccagcgt
     3121 agagaattct cgccctgcct atcttctgac ccttcttaat gggagcgtcc accttcttcg
     3181 gagtcagctc ctcgattacc tcaaagtaaa ctccctgagc gttgactccc gacgggacaa
     3241 gcgcagtaat ctccttttcg ggcaagagcc ttaaatggtc gtacttggag gagagcccaa
     3301 catcaattac gctgaccacc tgattctttt cagccacaag cctcagctta atattcttaa
     3361 atgtccagtc aagaatcttc ttgcactcca taaaggcgat gttttcttcg accccgtcat
     3421 tgtctatgtc ataaaaggga gcgcgcatta cgacagccag ataggtgtag ccgtctttgg
     3481 acgcctttgt gacaatgcag cgtcccgcgt tcgaggtggt tcccgtctta atgccctttg
     3541 cggtggggat gtagtaatcc ttaatgcctg aattcattat ctttacggtg gagataagat
     3601 ttctctcctg aggatatttg tttgtgggcg ggattgtgta tttctttgtg cttgtaatct
     3661 cctcaaaaat cgggttttca agggccttta atgtaaattt ggcgacatct ctcgcggtgg
     3721 tgtactgacc ctccgcgtca agaccgctgg ggttaataaa taccgtattc tcgcagccaa
     3781 tgctctttac atactcgttc atcatctgca caaaggcgcc gatgttgttg gggcagatgt
     3841 agtcggcaag cacattcgcc gcgtcgttgg cgcttgccac aagcatgcag taaaggagat
     3901 tccttacgct catctgctcg cccggcaaaa gccccgctgt ggagctgtta gtgccgtcaa
     3961 gcagtctgat ggcgtagttg ggaacctcca caatctcatc aaggttttgc gcattttcaa
     4021 gcacaatgga agcggtcaca atctttgcaa gggacgcggg agcggtaagc atgtccgcgt
     4081 tcttgtcaaa aataacggtg ccgtcgtcca tgctttgaag aaatacaatc tcagcctcca
     4141 catcaatctc gctgttgtag gaggcaagag cgtttacaga tgttgtaagt attataaggc
     4201 aaaccaaagc cgaaattaaa aacttcctca tagacaaatt cccgataagt gatataatta
     4261 tttacaatat attgtacttg aaaatccatt aaagtaaaga cttaaaagtt ttggcgggtg
     4321 aactgatgat atatattaca ggagataccc acggtgatga aagcaggctc tcgaccagaa
     4381 ggctgaagaa actgggggag ggcgataccc tcattatctg cggcgacttc ggctttatct
     4441 gggacggcag taaaagagag cagaaattcc ttgcaaagct cggctccaga aagtacaata
     4501 tctgctttat tgacggagcc catgagcgct ttgagctttt aaacaaatac cccgtagtcc
     4561 agtggaacaa gggctgggca aggcagattt cggggaatct tatgtactta atgcgcggcc
     4621 agatttatga gatagaaggc aagaaaatct ttaccatggg cggcggagag acccttgagt
     4681 ttgacctcag ggacgacagc ggcctatgga caagggcgga gctgcccagc aaggaggagc
     4741 tccttgaggg catcgaaaac attgagcgag tgggctttga ggtggacctt gtaatcaccc
     4801 acgaaccgcc cgcaaaggtc aaggagtttt taagactgcg cgacagcgag cccgtcagag
     4861 tctccgcctt caacgcctat ctgcaggagt tttctaccct ttgcagatat aaaaagtggt
     4921 atttcggctc aatgcacatt gacaagtaca tttcagcctc ccagaccgcc gttttcaagg
     4981 acattgtgga agccttcagc ggcgaaaaaa tctaaattaa agcggtttta caacagaaat
     5041 cagactgctt ttagcgtccg ctgccgtgta cccgccgggt gtgcggcaga aattctttat
     5101 gctgtcgttc catctgaggg cgattttatc aatctcctct tgtgagagcc ttagctcagg
     5161 aatggcggca agccttttta taagggcgac gaaatcgtca aagttcatat tggcagcctt
     5221 taaaaccttt tctgtctttt cgggcatatg ctttctgccc ctctccaaaa actccggagt
     5281 aaaggcggcg catgccctgc cgtgggggat attaaagcgt tctgtcagaa tatagcccac
     5341 cgtatggggg aagcaggttc ccgtaatgtt cagggagtaa cccgcaataa gcgacgctgt
     5401 gtataaaagg tctcttgtgt cctcgtttat ttcctcagcg ctctgaatct tgtccagacg
     5461 ctgcaggccc tctgtcagaa gctctacagc cttataagcg taaaggtcgg acatgtcgtt
     5521 ggcgtcaagg cagagtatgg actcaagtgc atgagccatg gcgtcaagag cggtggatac
     5581 ggtcaccgaa tagggcatgg agcttgtgta acttgggtcg cagaaggcga ctttagcata
     5641 gtaagccttt ccgcccacgc cctttttaaa gccggttttt gagtttgtca gaaccgaaac
     5701 cgctgtaacc tcgctgcccg ttcccgctgt ggtgcccaca agagctgcag gcaggggagt
     5761 gttctttatt tcagccgtat aaatatcgtc gggctccatg tgcctgttcg aggcgaaaac
     5821 ggctattgcc ttcgaagcgt caaggggaga gcctccgccg atgccgagga tgaaatcagc
     5881 cttgaactca tatgcggctg tgccggcgct gtggcaggtg gaagtcaggg gattttcctc
     5941 tatgcggtca aatatctcat attcaatgcc ctcctgactc aataccttta cggcgtcgtc
     6001 aagggcgccg ctctttttgg cggcggtggg gcttgtaact ataagacagc ggctgccgag
     6061 ctttttaagc tctttgccgt tttccaaaag cgcgttcttt ccgtaaaaca catctacggg
     6121 catatatgat gatatgttca taaattacct cccaaataaa ggatactgat aacattataa
     6181 cctatcataa aaaattatca atgacggaga atgtatgaaa cccgaaacct tagagaaaaa
     6241 tcttgaaccg aaaacgacag aaattaaaaa acccttaaaa gacagcctga aaacagcctt
     6301 taagctgagc ctgcccgtca tggcgggcta tatcttcata ggc
//
LOCUS       DAONVQ010000110         6341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9201_length_6341_cov_4.913777, whole genome shotgun sequence.
ACCESSION   DAONVQ010000110 DAONVQ010000000
VERSION     DAONVQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9201_length_6341_cov_4.913777"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..2384)
                     /locus_tag="PLD98_07245"
     CDS             complement(60..2384)
                     /locus_tag="PLD98_07245"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HQA06072.1"
                     /translation="MSNMVKIKVNGREVTADPSLNLLQNLLNNGIFVPNLCYRKELEA
                     YGGCGLCLVEIKGSPKLVRACNAMPAEGMEIETDTDRIRQSRRFTLALILSDHKADCI
                     APCKRACPTNQDAQGYIGLISAGKDRDAMALIMRDNPLPMSIGRVCPHPCESACRRQL
                     ADEPISICHLKRYAADKNTDYIPECAEDTGKRVAVVGAGPAGLSAAYFLRLKGHKVTV
                     FEAQEKAGGMLRYGIPAYRLPKDVLDKEIERIEKMGAEFKYGQKLGKDISLSSLKKEY
                     DAVFLAVGAWKSSSLGCDGEDHPNVLGGIDFLYKAANGEEVKLGKRVAVVGGGNTAID
                     AARTARRLGAEDVTLIYRRTRAEMPAEKIEIEEAQEEGVNFKFLAVPIAVCDGESCVL
                     LNLQQMTLGEPDATGRCAPVPIEGAVDVESFDTVIAAIGQRVLLDGLDDIELTKRGTI
                     EVDPETYATNIEGVFAGGDAVNRGPDIAVSAIAHGKNAAAAINSYLEGELKPVRKPFY
                     VERRDFTEKDLPEIEKEPRVKVTITEPEKRVNNFKEIAPCYTEDEAIKEASRCLECGC
                     SYLYDCRLLPLLQEYDPAGLGIKGTMRDSKVDKSHPFIYRDNKKCILCGLCIRTCAEL
                     VGKNVYGYNGRGFETEPECAFGIGLGESQCITCGACVRACPTGAIQSRLPFEKTPALQ
                     FETKTMECPYCEKKCLLSVDYFGSRILKSNPLVPPKACNVGKYLVPLSAYENLDKLTK
                     EQKEAIYRRIKDASAKFEGEFPADFEGLIKLIKK"
     gene            complement(2377..4143)
                     /locus_tag="PLD98_07250"
     CDS             complement(2377..4143)
                     /locus_tag="PLD98_07250"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQA06073.1"
                     /translation="MRFMIGYGSCGIAAGAEEVAKSVRERLSAEHSVTVDLVGCNGMC
                     FAEPLMQVKDDEGNDYYYGRLTGEKAVKIVDAFLNGEIPEENKIEGDELKFLKGQTRI
                     ALRHCGIIDPESIDSYIEAGGYTAFDKILKMTPDEVIEQIKISGLRGRGGAGFPTWFK
                     WEAARKENTEEKYIVCNADEGDPGAFMDRSVLEGDPHSVLEGMMVAGYAIGAKCGIIY
                     VRAEYPLAVKRLTIAINQLREKGLLGKNIKGSDFEFDFKIMKGAGAFVCGEETALLAS
                     LGGDRGMPTPKPPFPAQKGFRKQPTNINNVETFANVPWIIENGGEAFAAMGTEDSKGT
                     KVFALAGKVKYGGLVEVPMGMTIRQIVFDICGGIKNGKKIKGVQIGGPSGGCLPEELL
                     DTPIDYKSIGATGAIMGSGGLVVMDEDTCMVDMARFFMDFTSKESCGKCVPCRVGTTR
                     MLEILNRIVKGEGREGDMELLQSLGSSIRDSALCGLGNSAPNPVLTTMRYFKDEYTAH
                     IEEKCCPSFKCASLIKYEINPEKCTGCTLCGRKCPTKAITGSVKNPHVIDKDKCIACG
                     ECAASCRFGAIFKVSGEAVRYE"
     gene            complement(4144..4641)
                     /gene="nuoE"
                     /locus_tag="PLD98_07255"
     CDS             complement(4144..4641)
                     /gene="nuoE"
                     /locus_tag="PLD98_07255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HQA06074.1"
                     /translation="MKIKPQIDFSVVDEIIGRYENPSESLISLLQDIQEEYGYLPTDV
                     MVRISEQAGIPLSRLMGVATFYSQFKLNAPGKYKVLVCMGTACHVNNGERVADAVAKT
                     LGVKEGETTADGLFSWEEVACLGCCSLSPVMMINGQVYGKLDGKKVEQVINDIRKAEL
                     ERAGQ"
     gene            complement(4759..5763)
                     /locus_tag="PLD98_07260"
     CDS             complement(4759..5763)
                     /locus_tag="PLD98_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06075.1"
                     /translation="MNFPDLRAERWIDCEKNPIIGYFHGEKSRYAIGDPQILTPDEFD
                     GKWHMLYHGFFDDYLPYLYHVVSDDGIEWRFQKRWQINVGPVCLFKDNDRFILYSSEV
                     SWRAVKAEGLPKDYYGKGTAYIIRARTSTDLEEWSEPIDIIIPEFDWEKTGPEACVRN
                     PCMVRLKDGRYRLYYSGGSVRLPVCGYPEPYAIGYAESDSPLSGFVKREQPILTPDEN
                     IPYRNFGCGGIKVYGWEDKFLALYNPICMDKEGLPRSAIAALVSDDGINFEEYPLNPI
                     ISPAADGWKKALVYQLDVALHNGEVRIYYNARDEWLDGIERIGMSAIYNPELKIRKLY
                     "
     gene            complement(5972..>6341)
                     /locus_tag="PLD98_07265"
     CDS             complement(5972..>6341)
                     /locus_tag="PLD98_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HQA06076.1"
                     /translation="DVAIASPDMMGLVGRLGKILGPRGLMPSPKAGTVTPDVARAVNE
                     AKAGKIEYRLDKTNIIHCPIGKVSFGKEKLAENFYTLLDAIIKAKPAAAKGQYIRSCV
                     VATTMGPGIKINPNRVTKEA"
BASE COUNT         1338 a         1681 c         1636 g         1686 t
ORIGIN      
        1 ataacggcgg gggaaataat tcaacaacaa aaaagcgcag cttaaggctg cgcttttatt
       61 tatttcttta tgagctttat aagaccttca aagtctgcgg ggaactcgcc ctcaaatttt
      121 gccgaggcgt ccttaatgcg cctgtaaatg gcctcttttt gctcttttgt gagcttgtcg
      181 agattttcat atgccgacag aggaacgagg tatttgccca cattgcaggc tttgggcggg
      241 acaaggggat tagactttaa gatgcggctg ccgaagtaat ctacgctgag aaggcacttc
      301 ttctcgcagt aggggcactc catggtcttt gtctcgaact ggagcgcggg ggtcttttca
      361 aagggcagcc ttgactggat ggcgccggtg gggcatgctc tgacgcaggc tccgcaggtt
      421 atgcactggc tctcgccgag tccgatgccg aaagcgcact cgggctctgt ttcaaagcct
      481 ctgccgttgt agccgtagac attcttgccc acaagctcgg cgcaggtcct aatgcacaga
      541 ccgcagagta tgcacttttt gttgtccctg tagatgaagg ggtgactctt gtccaccttg
      601 gaatcacgca tggtaccctt aatgccgagc cctgcggggt cgtactcctg aaggagaggc
      661 agcaggcggc agtcatagag ataggagcag ccgcactcaa ggcatcttga cgcctctttg
      721 attgcctcat cctctgtata gcagggagca atttccttga aattgtttac cctcttttcg
      781 ggctcagtga tggtgacctt tactctcggc tccttttcta tttcgggcag gtctttttcg
      841 gtgaaatccc tgcgctcaac atagaagggt ttgcgtacgg gcttgagttc gccctcaaga
      901 tagctgttga tagcggccgc ggcgtttttg ccgtgggcta tcgcagagac cgcgatgtcg
      961 ggacccctgt ttaccgcgtc gccgccggcg aacacgcctt caatattggt ggcgtaagtt
     1021 tcggggtcaa cctcgattgt gccgcgcttt gtaagctcga tgtcgtcaag accgtcaaga
     1081 agcactctct gaccgatggc ggcgatgacg gtatcgaagc tctccacatc gacggcgcct
     1141 tcaatcggaa cgggggcgca gcgtcctgtc gcgtcgggct cgccaagggt catctgctgg
     1201 agattcagca gcacgcagct ctcgccgtcg caaacggcta tggggaccgc aaggaacttg
     1261 aagttcacgc cctcctcctg agcctcttca atctctatct tttcggcggg catttccgct
     1321 cttgttcttc tgtaaataag ggttacatcc tctgcgccaa ggcggcgggc tgttcttgcg
     1381 gcgtcaatgg ctgtgttgcc gccgccgact accgctaccc tcttgcccag ctttacttcc
     1441 tcgccgtttg ccgctttata aaggaagtct atgccgccga ggacattggg atggtcctcg
     1501 ccgtcgcagc cgagtgacga gctcttccat gcgcccacag cgaggaatac ggcgtcatat
     1561 tcctttttaa gggaggaaag acttatatct ttgccgagct tttgaccgta tttaaactcg
     1621 gcgcccattt tttcaattct ttctatctct ttgtcgagga catcttttgg gagtctgtaa
     1681 gccggaatgc cgtagcggag catgccgccc gctttttcct gagcctcaaa gacggtgacc
     1741 ttgtgaccct tgagccttaa gaaatatgcg gcggaaagac ccgcgggacc ggcgcccaca
     1801 acggctaccc tcttgcctgt gtcctctgcg cattcgggaa tatagtctgt gtttttgtcc
     1861 gctgcgtatc ttttaagatg gcagattgaa ataggctcgt ccgccagctg tctgcggcat
     1921 gcggattcgc aggggtgggg gcatactctg cctatggaca tgggcagggg gttatccctc
     1981 atgataaggg ccatggcgtc cctgtccttg cctgcggaga taagaccgat atagccttga
     2041 gcgtcctgat tggtggggca tgccctcttg cagggggcga tacagtccgc cttgtggtcg
     2101 gagagaataa gggcaagggt gaacctgcgg ctctgcctta ttctgtcggt gtcggtttca
     2161 atctccatac cctccgcggg catagcgttg caggctctga cgagcttggg agagcctttg
     2221 atttccacaa ggcaaagtcc gcagccgccg taagcctcaa gctcttttct gtagcagaga
     2281 ttgggcacaa agatgccgtt gttaagaagg ttttgaagca ggttgagcga ggggtcagcc
     2341 gtcacttccc tgccgttgac ttttatttta accatattac tcatacctca ctgcctcccc
     2401 gctgaccttg aatatcgcgc cgaatctgca cgaggcggcg cattcgccgc aggcaatgca
     2461 cttatccttg tcgattacat ggggattctt tacgctgcct gtgattgcct ttgtggggca
     2521 ttttctgccg caaagggtgc agcctgtgca cttttcggga ttaatctcgt atttaatgag
     2581 cgaagcgcat ttgaaagagg ggcagcattt ttcctctata tgcgctgtgt attcgtcttt
     2641 aaagtatctc attgtggtga gaacaggatt gggggcggag ttgcccagtc cgcaaagggc
     2701 ggagtctctg atgctggagc caaggctctg gagcagctcc atatcgccct ctctgccctc
     2761 gccttttacg attctgttaa gaatctcgag catcctggtg gtgcctactc tgcagggcac
     2821 gcacttgccg cagctttcct tgctggtaaa gtccataaag aaccttgcca tatccaccat
     2881 gcaggtgtcc tcatccataa cgacaagtcc gcccgagccc atgatggcgc ccgttgcgcc
     2941 tatgctctta tagtcgatgg gggtgtcaag cagctcctct ggcaaacagc cgcctgaagg
     3001 accgccgatt tgaactccct tgattttttt gccgttcttg atgccgccgc agatgtcgaa
     3061 cacaatctgc cttatggtca tgcccatggg gacttcgacc agtccgccgt actttacctt
     3121 gcctgcgagg gcaaagacct ttgtgccctt gctgtcctcg gtgcccatgg cggcgaaagc
     3181 ctctccgccg ttctcaataa tccaaggcac attggcaaag gtttcgacat tgtttatgtt
     3241 cgtgggctgc tttctgaagc ccttttgagc ggggaaggga ggcttgggtg tgggcattcc
     3301 cctgtcgccg ccgagggagg caagcagagc cgtttcctcg ccgcagacaa aggcacccgc
     3361 gcccttcatt atcttaaagt cgaactcgaa gtccgatccc tttatgttct tgcccaaaag
     3421 acccttttct ctgagctgat ttatggcaat agtcaatcgc ttgaccgcga gggggtactc
     3481 ggcgcggaca tagatgatgc cgcactttgc gcctattgcg tagcccgcga ccatcatgcc
     3541 ctcaaggacg ctgtgggggt cgccttcaag aacggacctg tccatgaatg cgccggggtc
     3601 gccctcgtcg gcgttgcaga cgatgtactt ttcctctgtg ttttctttcc tcgcagcctc
     3661 ccacttaaac catgtgggga agcctgctcc gcctctgccc ctgagaccgg atatcttaat
     3721 ctgctcgata acctcatcgg gggtcatttt aagtatcttg tcaaaggctg tgtatccgcc
     3781 agcctctata tatgaatcaa tgctttcggg gtcaataatt ccgcagtgcc tcagggcgat
     3841 tctggtctgt cctttaagga acttcagctc atcgccttca attttgtttt cttcgggaat
     3901 ttctccgttt aagaaagcgt caactatttt tacagccttc tcaccagtga gtctgccgta
     3961 gtaataatcg ttgccctcat catcctttac ctgcattaaa ggctcggcaa agcacatgcc
     4021 gttgcagccc acaaggtcga ctgtaaccga gtgttcggcg ctcagccttt cacgaacgga
     4081 ttttgctact tcctcggcgc cggcggctat gccgcagctg ccgtaaccta tcataaacct
     4141 catttattga ccagcccttt caagctctgc tttgcgaata tcgttaataa cctgctccac
     4201 ctttttgccg tccagcttgc cgtatacctg accgtttatc atcattactg gtgaaaggct
     4261 gcagcagcca aggcaggcga cctcttccca ggagaaaaga ccgtcggcag tggtttcgcc
     4321 ctctttgacg ccaagagtct tagcgacggc gtccgccacg cgctcgccgt tgtttacatg
     4381 gcaggctgtg cccatgcaga caagcacttt gtacttgccg ggggcgttca gtttaaactg
     4441 ggagtagaag gttgcaacgc ccataaggcg gctgagaggt ataccagcct gctcgctgat
     4501 tcttaccata acatccgtgg gcaaatagcc gtactcctcc tgaatgtcct gaagaagaga
     4561 gataagggat tcggacgggt tttcgtacct gccgattatc tcgtctacaa cagaaaaatc
     4621 tatttggggt ttgattttca agagtatccc tccaaaatcg cggcgatgta tcacaaaagg
     4681 cagacacctg agatgtatct gccctaatat ccgccgtatc agaatttcaa tacaagaaag
     4741 ccctttcggg cttaaacact aataaagctt gcggatttta agctcaggat tatagatagc
     4801 gctcatgccg attctctcta tgccgtccag ccactcgtcc cttgcgttgt agtaaattct
     4861 aacttccccg ttatgtaaag ccacatcaag ctgatacaca agggcttttt tccacccgtc
     4921 agcggcggga gaaattatag ggttgagggg gtattcctca aaatttatgc cgtcgtcgga
     4981 aacaagggcc gctatcgcgg agcggggcaa accttcttta tccatgcaga tggggttata
     5041 gagggcaagg aatttatcct cccagccgta aaccttaatg ccgccgcatc cgaaattgcg
     5101 gtagggtata ttttcgtcgg gagtcaaaat gggctgttcc ctctttacaa agcccgatag
     5161 cggggaatcg ctttcggcat agcctattgc gtagggctcg ggataaccgc acaccggcag
     5221 gcgaacggag ccgccgctgt agtaaaggcg gtatctgccg tctttgagcc ttaccatgca
     5281 ggggtttctg acgcaggctt cgggtcctgt cttttcccag tcaaactcgg gaataattat
     5341 gtcaatgggt tcggaccatt cctcaaggtc ggtgcttgtc cttgccctaa ttatataagc
     5401 ggtgcctttg ccgtaataat cttttggcag accctccgcc tttacagctc tccacgaaac
     5461 ctctgaggag tagagaataa agcggtcatt gtctttaaac aggcagacgg gaccaacatt
     5521 gatttgccag cgtttttgga atctccactc gatgccgtca tcggatacca catgataaag
     5581 gtagggcaaa tagtcatcaa aaaagccgtg gtacagcatg tgccacttgc cgtcaaactc
     5641 atctggcgtt agaatctgcg ggtcgccgat ggcgtatcgg ctcttttcgc catggaaata
     5701 gccgatgatg ggattttttt cgcagtctat ccaccgctcc gcccgtaaat cgggaaagtt
     5761 catttttttc tcctaaaaac tcttgcgcca aagagcagca ttagaatttt aacatactcg
     5821 cgcgctttgg aacaagaaag agattttggt atttatatac cctataattt tatgtgtatt
     5881 ttgcttttca gtcaagaaac gataataaat taacaacaaa aaacgcacct gcctttgcag
     5941 atgcgcttta gtcttatact ctaagccgga attaagcctc ttttgtaact ctgttggggt
     6001 taatcttgat tccggggccc attgttgtag caacaacgca ggaacggata tactgaccct
     6061 ttgcggctgc gggcttagcc ttgattatag cgtcaaggag tgtatagaag ttctctgcca
     6121 acttttcctt gccgaaggaa accttgccga tggggcagtg aatgatgttg gtcttgtcaa
     6181 gacggtactc aatcttacct gccttagctt cgttaacagc tcttgcgaca tcgggtgtaa
     6241 cggtgcctgc cttggggctg ggcataagtc cgcggggacc caaaatctta ccgagacggc
     6301 caacgagacc catcatatcg ggggatgcga ttgctacatc g
//
LOCUS       DAONVQ010000111         6335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9213_length_6335_cov_4.221497, whole genome shotgun sequence.
ACCESSION   DAONVQ010000111 DAONVQ010000000
VERSION     DAONVQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9213_length_6335_cov_4.221497"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PLD98_07270"
     CDS             <1..570
                     /locus_tag="PLD98_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQA06077.1"
                     /translation="IAVLNYYDGSAPKYVVAPKRTRFINFEIITALEDYENPKNYSLD
                     CLYEVSCGAVVAREINNEIRYLLIKNKRSNHWGFPKGHMEKGETKKQTAAREVFEETG
                     INIKFIEGFVSTSEYKIGGKVEKSVTIFLASTNDTKTVIQKTEIEDYVWLAYEQAMEN
                     LRFENDRLILEKANEFLKERAEKVFGKKL"
     gene            718..2091
                     /gene="asnS"
                     /locus_tag="PLD98_07275"
     CDS             718..2091
                     /gene="asnS"
                     /locus_tag="PLD98_07275"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HQA06078.1"
                     /translation="MKRTEIKDVLSGNLLSKKVTVCGWVKTSRESKTVAFAEINDGSS
                     FSPVQIVIDKAALEDTLKFLKVGAAIKVTGTVVKSEINDQIEINAEEIILLGESPSDY
                     PLQKKRHTLEFLRTMPHLRIRTNTFNAVFRVRSKISFAVHEYFQKNGYYYVHTPIITS
                     SDCEGAGEIFRVTTLPPFYASPEGEDYYKEDFFGKKAGLTVSGQLEGEAAALALGKIY
                     TFGPTFRAENSNTPRHAAEFWQIEPEVAFAELPDIIEIAEDMIKYLIRYALENCREEL
                     EFFEKFYEKGLIEKLEKVVSEKFGVIEYTEALELLKKADADFKYPVEWGLDLQTEHER
                     YIAETVFNRPVFVINYPKDIKSFYMKQNPDGKTVAATDLLVPGVGEIIGCSERENDYD
                     KLLSAIRSRGMNEDDYKSYLELRKFGSAPHSGFGLGLERFLMYITGISNIRDVQLYPR
                     TPKEIRV"
     gene            2318..3373
                     /locus_tag="PLD98_07280"
     CDS             2318..3373
                     /locus_tag="PLD98_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06079.1"
                     /translation="MKNLICDKCGSSEFERKDGKYVCLYCRSKVAPEQEKTLRVSMDS
                     RGSKVKFDASLELSNLYELARRYLEIGDYEKALKYYEMILLQDPTNWEATFYIEYIEA
                     AKCKLHEIYREGASLNNCINPVMRLIKNHISSKDEQIRGVNEVVTRCFELSNLFYRSS
                     VNHFNSLMADAKKAFLAAHNDNLLVARTIPYALGDSIESCFGNYPELHPIMVNSWKEG
                     IVKHVGSLKYLRIRYSNKTIKENIAIIKAYEAKVKRFEPAYNHPNLNGCYVATAVYGS
                     YDCPEVRTLRRFRDNTLSKSVWGRAFIHTYYAISPTLVKWFGGTAWFTRFFRKRLDRF
                     VAKLNQEGIENTPYQDK"
     gene            3525..4556
                     /locus_tag="PLD98_07285"
     CDS             3525..4556
                     /locus_tag="PLD98_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HQA06080.1"
                     /translation="MKRKNEDLSEACFIRRSGSDKSILITGIPGCDKDVILSALSGLN
                     IDSGGWNGNGSCSSQIHIKYKGKQIDLLSLPETYSLWLSGNGAGFTGNCFAFADYDAF
                     IIVCKSGSIAYGESLYAEATKFCPEGYLCINSLSGNFRFQFKRFLSAPFQSKYGKAGA
                     KGSNQSLAGSADSCIFNAGSKCKYKDIKPLDYEEIPLLSCFEFRSKTEILHKAKRIEV
                     SGIKDIKRLKKVLFDTENLDGAGKKTAAVSDEVLSAVNLPQLFEPLIAMLTGLNPNAD
                     PYLEAIKFVLLAQEGVKLPFACFLPVQLNLTKEFIMCVNKICHQLNEHNIRLKDCSLA
                     VKDKLKEDM"
     gene            4901..5554
                     /locus_tag="PLD98_07290"
     CDS             4901..5554
                     /locus_tag="PLD98_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06081.1"
                     /translation="MRLKKFFVLSVALFMCFVFPIASLAAEGDKEPALDDFTGGLSGI
                     GDFFGGIGDFDLSKIKDIVPGADKISDFFGGIGGGLFDGIGGLLPGFGSKPTTTETEP
                     VLDTTEPTIPELPTLPQSPTMPSTSSSYGRTTTKPTTTEDYEPVTETTTEANAEESSS
                     QNTYTRPSTTVNYTDNVDSIRFDSGDTMSTPFKIGLGAGMLAVSIGGIVLIVLKFKN"
     gene            5639..>6335
                     /locus_tag="PLD98_07295"
     CDS             5639..>6335
                     /locus_tag="PLD98_07295"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HQA06082.1"
                     /translation="MEISVELTKTPKKKPSENEPLGFGKYYTDHMFIMNYDEGKGWHS
                     PRIVPYQPISLDPAAMCFHYAQEVFEGLKAYRTKKGDIQLFRPEKNMERLNLSNDRLC
                     IPRIDEEFAVEAIKKLVSVDKDWVPHGEGTSLYIRPFIFAVDPYVGVHPAKHLLFVII
                     LSPVGSYYPEGINPVKIYVEDEYVRSVRGGMGYVKAGGNYAASLKSQDKAQKLGYTQV
                     LWLDGVERKYVEEV"
BASE COUNT         1850 a         1475 c         1461 g         1549 t
ORIGIN      
        1 atagctgttc tcaattacta tgacggttct gccccaaagt atgttgttgc cccaaagcgc
       61 acaaggttta ttaatttcga gattattacc gcccttgaag actatgaaaa ccccaaaaac
      121 tactcccttg actgtttata tgaggtttcc tgcggcgcgg ttgtcgcaag ggaaatcaac
      181 aatgagataa ggtacctgct tataaaaaac aaaagaagca accactgggg cttccccaag
      241 ggtcatatgg aaaagggaga gaccaaaaag cagaccgccg cgagagaggt ttttgaggag
      301 acgggcatta acataaagtt tattgagggc tttgtgtcca cctccgagta caaaatcggc
      361 ggcaaggtgg aaaaatcggt caccatcttc ctcgccagca caaacgacac aaaaaccgta
      421 attcaaaaaa ccgagataga ggactatgta tggctcgcct atgagcaggc tatggagaac
      481 ctgagatttg aaaacgacag gctgattctt gaaaaagcca acgaattctt aaaggaaaga
      541 gccgaaaagg ttttcggcaa aaagctctaa ataaaattac aaacgccctg ccgcctaagc
      601 gcggcgggtt ttgttttgcc gctggcacat aggatagaaa tttcgggaaa taaaaatgtt
      661 gaaaatgatg acggatttgg tatcataatt atgtcaaaag ttattcggag gtgccctgtg
      721 aaaagaaccg agattaaaga tgttttaagc ggaaatctgc tgtctaagaa agtcaccgtt
      781 tgcggctggg tcaaaacctc aagggagtct aaaaccgtcg cctttgcgga aataaacgac
      841 ggctcctcat ttagccccgt tcagattgtc attgacaaag ccgccctcga agataccctc
      901 aaattcctta aagtcggcgc cgccataaag gttacaggca ccgttgtaaa gtcggaaatt
      961 aatgaccaga ttgaaataaa cgctgaggaa ataatcctcc tgggcgagag cccctctgat
     1021 tatcctttgc agaaaaagcg tcacactctt gaattccttc gcactatgcc tcaccttaga
     1081 atcagaacaa acaccttcaa cgcggttttc agagtccgtt ccaaaatctc ctttgcggtg
     1141 catgagtatt ttcagaagaa cggctattac tatgtccaca cgcccataat cacctccagc
     1201 gactgcgagg gcgcggggga gattttcaga gtcacaaccc tcccgccttt ctatgcctcg
     1261 ccggagggag aggactatta caaagaggac tttttcggca aaaaagcggg tcttaccgta
     1321 tcgggtcagc ttgaaggcga ggctgcggct ctggctctgg gcaaaatata caccttcggc
     1381 cccacattca gagccgagaa cagcaacacg ccccgccacg ccgccgagtt ctggcagata
     1441 gagcccgagg tagcttttgc cgagcttccc gacataatcg aaatcgccga ggatatgata
     1501 aagtacctta taaggtacgc cctcgaaaac tgcagggaag aactcgaatt ctttgaaaag
     1561 ttctacgaaa agggtcttat cgaaaaactg gagaaagtgg tttccgaaaa attcggagtc
     1621 atcgaataca ccgaggcgct agagcttttg aaaaaagctg acgcggactt taaatacccc
     1681 gtggaatggg gacttgacct gcagaccgag cacgaaaggt atattgccga aaccgtcttt
     1741 aaccgccccg ttttcgttat caattacccc aaggatataa agtccttcta tatgaagcaa
     1801 aatcccgacg gcaagaccgt cgcggcaacc gacctgcttg tccccggcgt gggagagata
     1861 atcggctgca gcgaaaggga gaacgattac gataagctcc tcagcgccat aagaagcagg
     1921 ggaatgaacg aggacgacta caagtcatat ctggagctga gaaaattcgg ctccgccccc
     1981 cacagcggct tcggcctcgg ccttgagcgt ttcctcatgt atatcacggg aatctcaaac
     2041 atccgcgatg tccagctcta ccccagaaca cctaaggaaa ttagagttta aaaagaggac
     2101 gccgaagggc atcctttaat tttcccgctt ttggggaccg tcaattattg acactaataa
     2161 aaataaattg taaactttct tcatagtatc gaatttcggc gaaaacagaa aattttgccg
     2221 agatttaatc ttttcggctt tataaatgtg tttgcgattc atacagccga gcaaagcttg
     2281 cataattttt ttaaataaca gatttagggt gcgtgtaatg aagaatctta tttgcgacaa
     2341 gtgcggaagc tcggagtttg aaagaaaaga cggcaaatat gtctgtcttt actgcaggag
     2401 caaggttgct cccgagcagg agaaaacttt aagggtaagc atggacagca ggggcagcaa
     2461 agttaaattc gatgcctccc ttgagctctc caatctctat gagcttgccc gccgctactt
     2521 ggaaatcggc gattacgaaa aggccttaaa gtattatgaa atgattttgc ttcaggaccc
     2581 cacaaactgg gaagcaactt tctatattga atatatcgaa gcggcaaaat gcaagcttca
     2641 tgagatatac agagaaggcg catcgttaaa taactgcatt aatcctgtaa tgaggcttat
     2701 taagaatcac atcagctcta aagacgaaca aatcagaggg gttaatgaag ttgtaacccg
     2761 ctgctttgag ctttcaaatc tcttttacag atcttcggta aatcatttta acagcctaat
     2821 ggcggacgca aagaaagctt ttttggcagc ccataatgat aacctgcttg tagcaaggac
     2881 tatcccgtat gccctcggcg acagcattga gagctgcttc ggcaattacc ccgaactgca
     2941 tcccattatg gtaaactctt ggaaagaggg catagtcaag catgtcggtt cgctgaagta
     3001 ccttagaata aggtacagca acaagacaat aaaagaaaat atagcaataa ttaaggcata
     3061 cgaagccaaa gtaaaaagat ttgagccggc atataatcat cccaatctca acggctgcta
     3121 tgtggcgacc gcggtttacg gttcttacga ttgccccgaa gtccggacac tcaggcgttt
     3181 tcgcgacaac actctctcaa aatctgtttg gggcagagca ttcatacata cttattacgc
     3241 cataagcccc acgctggtaa aatggtttgg cggcacagca tggttcacaa ggtttttccg
     3301 caaaaggctt gaccgctttg tcgcaaaact gaatcaagag ggcatagaga acacccctta
     3361 tcaggacaaa taaacaaaca gcaaatatct atgaaaaact tagaccgcat catatccgat
     3421 gcggtctttt tgcgttttct attgaattcc gccgcctgct ttagtcatta aatcgcactt
     3481 ttgcttatag aatagtacaa ataccgacag gcaggtgttt gaaggtgaaa agaaaaaacg
     3541 aggatttatc ggaagcctgc tttatccgca gaagcggcag cgacaaatca atactgatta
     3601 ccggcatccc cggctgcgac aaggatgtaa tactgtccgc cctttcgggt cttaacatag
     3661 acagtggggg ctggaacgga aacggtagct gctcatccca aatacacata aaatataagg
     3721 gaaagcaaat tgaccttttg agcctgcccg aaacctattc cctttggctg agcggaaacg
     3781 gcgcgggatt tacgggtaac tgctttgcct ttgccgacta cgacgccttt attatcgtct
     3841 gcaaaagcgg cagcatagcc tatggtgaga gcctttatgc cgaggcgact aaattctgcc
     3901 ccgagggcta tctttgcatc aacagtcttt cgggcaattt cagatttcag tttaagagat
     3961 ttctgtctgc gccgtttcag tcaaagtacg gaaaagccgg agcaaaaggc tcaaatcaaa
     4021 gccttgcggg ctcagccgac agctgcatat tcaatgcggg ctcaaagtgt aaatacaagg
     4081 atataaaacc acttgattat gaagaaatcc ccttgctttc atgctttgag tttaggtcta
     4141 agacggagat tctgcacaag gcgaaaagaa tcgaggtgag cggcattaag gacataaaga
     4201 ggcttaaaaa agtcctgttt gatactgaaa atctcgatgg cgcaggcaag aaaacggctg
     4261 cggtatccga cgaagtattg tcggcagtca atctccccca gcttttcgag cctttgattg
     4321 ccatgctcac aggactgaat cccaatgccg acccctatct tgaggcaata aagttcgtgc
     4381 tgctcgctca ggagggggta aagctcccct ttgcctgctt tttgccggtg cagctgaatc
     4441 tcacaaagga gtttataatg tgcgtaaata aaatctgcca tcagttgaat gagcataata
     4501 ttagactcaa agactgctcc ctcgccgtaa aagacaaact caaagaggat atgtaataca
     4561 taagccccga aggaaatcgg ggtttttctt tttccgcagc aaaaagccga atttctgagt
     4621 ctaataaacg aacggatgat atgctgaaat tggctcttta ggcaaaaatt ttatcggcat
     4681 ttatttgcaa aaaccctcta tgttgcttga ctattgccgt caaataggat aaaatgcaag
     4741 taatatcaat gtattttagg gattttaggc tgtggatgtc gtgaaaatct atttgttata
     4801 gcccttattt tgcaatcaaa acagagctat tagcaaacgg gcatcttacg gctttttatt
     4861 taataacttt ttgcttacta cctttttgag aggaatagat atgcgtttaa agaaattctt
     4921 tgtgttgtcg gtcgcgctct ttatgtgctt tgtgttcccc atcgcctctc tcgcggcgga
     4981 gggggataag gagcctgccc ttgacgattt caccggcggt ctttcgggaa tcggcgactt
     5041 tttcgggggc atcggcgact ttgatttgtc taaaatcaag gacattgtcc ccggggccga
     5101 taagatttcg gacttctttg ggggcatcgg cggcggtctg tttgatggca taggcggttt
     5161 gctccccggc tttggcagca agcccaccac aaccgaaacc gagcctgtgc ttgacaccac
     5221 agagcctact attcccgagc tgcccacact tcctcagtct cccacaatgc ccagcacctc
     5281 aagctcctac ggcagaacga ccacaaagcc cacaactaca gaggattatg agcctgtaac
     5341 cgagacaacc accgaggcaa atgccgagga atccagctct caaaatacat acacccgtcc
     5401 ttccacaacc gtaaattata ccgataatgt ggactcaatt cgctttgaca gcggcgacac
     5461 aatgagcact cccttcaaaa tcggtctcgg cgcaggaatg cttgcggttt caatcggcgg
     5521 cattgttctg attgtattaa agtttaaaaa ctaattttta gcgaaccgaa tattatgaga
     5581 ttttcaggac ggcacccacc gtcctgaaaa aaatatttta attggagatg gtaaaatgat
     5641 ggaaatcagt gtcgaactta caaaaactcc gaagaaaaag ccctccgaaa acgaacccct
     5701 gggattcggc aaatactaca ccgaccatat gttcattatg aactatgacg agggcaaagg
     5761 ctggcacagc ccccgaatag tgccctatca gcccatctcc cttgaccctg ccgcaatgtg
     5821 ctttcattat gcccaggagg tatttgaggg actgaaggct tacagaacaa agaagggcga
     5881 cattcagctt ttccgccccg aaaagaacat ggaaaggctc aatctctcca acgacaggct
     5941 ttgcattccc cgcattgatg aggagtttgc cgttgaggca attaagaagc ttgtttccgt
     6001 ggacaaagac tgggtacccc acggagaggg cacatccctt tatatcaggc ccttcatttt
     6061 cgccgttgac ccctatgtgg gagtgcatcc ggcaaagcac ctgttgttcg tgataattct
     6121 ttctcccgtc ggttcctatt atcccgaggg cattaacccc gtaaagatat atgttgagga
     6181 cgaatatgtc aggtccgtca gaggcggcat gggctatgta aaagcaggcg gaaactatgc
     6241 cgcgtccttg aagtcacagg acaaagccca gaagctgggc tatactcagg ttctctggct
     6301 tgacggcgtt gagcgcaaat atgtcgaaga agtcg
//
LOCUS       DAONVQ010000112         6258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9352_length_6258_cov_5.041270, whole genome shotgun sequence.
ACCESSION   DAONVQ010000112 DAONVQ010000000
VERSION     DAONVQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9352_length_6258_cov_5.041270"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="PLD98_07300"
     CDS             <1..1462
                     /locus_tag="PLD98_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQA06083.1"
                     /translation="FISEGYSSQRIAILGENSYEWLLTWFAVVISGNTAVPLDKMLSE
                     EQLKFVLSDTGVSLIIYSKQFADFIPAFKDENELLKVLSMENDLGSCLEKGGKALAEK
                     NKTEVEIRPEPSDTAMIIYTSGTTGIAKGVMLSHNNLTFDAMGVSMHLESGGNNLFVL
                     PLNHAYAIIAILVVMNKGKPTCINSGLKRFAQEIKEFKPHEMYLVPLIVENLYDKIME
                     KAKAEGKLKQLKFLMKLSLFLYSIGIDIRRKLFKPILNEFGGNLSILVCGGAALDPDY
                     IKAFRAFGINVLIGYGISECSPVISVNRNEHWRDGSVGIPLKGVEVKTDAPDGKSEGE
                     LYVRGGIVMKGYWRNEKATAETFDGDWFKTGDIGCIDKDGFVYITGRKKNVIILSNGK
                     NLYPEELEMQLVKLPAIAEVLVYEENKKVTAEIFPDEDFINENDIQDVKEYIESVVEK
                     FNKQFPACKSIAAVKLRNTCFEKTSTKKIIRKRIAS"
     gene            1459..1677
                     /locus_tag="PLD98_07305"
     CDS             1459..1677
                     /locus_tag="PLD98_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HQA06084.1"
                     /translation="MKERILKILLSNPKIQNVTPESNLVTDFGLSSLDLAEIVCDIED
                     EFDIEIPEKDLVRIKTLQDIYDYIDTHA"
     gene            1695..2885
                     /locus_tag="PLD98_07310"
     CDS             1695..2885
                     /locus_tag="PLD98_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQA06085.1"
                     /translation="MKKKKGHSPLWYYVVGGIMGYSKIRGHNVKFRISDEVKNLKPPF
                     VGIANHQGYYDWAYAARAFFPMRFRFVVSRYQFLNPKTGKLLEKLNSIPKSQFTSDAS
                     AVKEIIRTIKRGENVFIFPSGRLSLFGEDDKPVEGTYELLKKLGVPVVMVHLDGSYRT
                     APRYNTEVKKGRVVVTAYVLFTPEELKNLPEEEAKKRLYSAFCHDDFNSENVCEFKSP
                     DIAKGLDDLLYMCPDCKANYTVKVMPGQKLMCKDCGFSVQMDNYFKFTALSGNASPKT
                     ITEWSHIIKAEEKKRILSDPDFTLREKMDVLEHIKEDEQLTKTGECEISLSRDGIKIE
                     GTKKDGRFSRHYTLKEYPAMHIFDKIYLLVPDNEAVICVSPANAKIATRWAVVSETLS
                     SLLL"
     gene            complement(2972..3754)
                     /locus_tag="PLD98_07315"
     CDS             complement(2972..3754)
                     /locus_tag="PLD98_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HQA06086.1"
                     /translation="MVEYTQILTWPDITIRVLAAIAIGLVIGIEREISNHPAGMKTHV
                     LVCLGSALASIIAAEMGLSIYLYPGARVDLSRIASGVVSGMGFIGAGAIMKSRDGTMV
                     TGITTAATLWATACMGLAVGMGYFRMAFIVFLAVFGTTVILKSVERRFFARLRTRRLE
                     LYITDKRTTIPQLDDYFERKKITVLSFEYLLSGDHFNSAGERISRCRYMIKVPKAVNF
                     NNIVREIALFENVYEVYENFPGKEETEDQPVIEKTDVLADNE"
     assembly_gap    3771..3870
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3940..>6258)
                     /locus_tag="PLD98_07320"
     CDS             complement(3940..>6258)
                     /locus_tag="PLD98_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06087.1"
                     /translation="PGTITSDELSKLDLEKTKAFTAVTEEMPQKRQRFIRTTESDPAH
                     TMVFHDELWDTLDRADTKKRQIARIKAIAESGALEAGEIEQDEEIPQQLFFSGFEPID
                     SSDRLEERKIEAALRKSRIEQAKIFNLYGENKWDDISSEEKKVPKKAVSDDADIEEIT
                     EKKKTQKTKAPRSKEKSKEKKAEGIVLEMPSASLGIYPEYRNPKDKKRIAGFLNLYKS
                     KQGAKAALFFALGIVSILLPLFYKVGEPLASELKNRLLASIILQLIILAGAFFVALPE
                     IRLGLNSAVKKQPDANSGVMLMLFAAAIQSLVMLFGYESLAHDLSLLAPSALILCSIV
                     FAGKYINALNIKRGFAFLVKNEDEMCAVADLGASDAYADIKRHLPLENPRIKYSAKPE
                     FISSFISESMGDSLSAKILSRGFALIVAEAVVAFIAATFKGDVFEGFFALAALAIISF
                     PVGVLPASSLGLLLENLKTAAKCYVTGFKAALNLTETDTFIIDANELFPPDACEIHGI
                     KTFGGMKLDKAILYIASTVIKAGGPLKPAFEKVIMGNDKLLLPVEALSYEDRLGLSAW
                     INEKRILVGNAQLLRNHSVKAPDDSFEKKYTEDGKRVIYLSVEGQLAAMLVVSYYPSA
                     AVKKGLNALVNQGITVLVTSRDSNITEEFIESSFGTGKNGVRVLTSASAEFFKECIEN
                     ERSKEVPASVIHKQGALSLFSAFASALKLAKTGKMLIPLFAAGGIAGAVIVLFMAIAG
                     YISKAGNIGIILLQLLWTVLGSGLAIFNNKRD"
BASE COUNT         1671 a         1525 c         1396 g         1566 t
ORIGIN      
        1 tttcataagc gagggctatt cctcgcagcg cattgccata ctgggtgaaa attcctacga
       61 gtggcttttg acctggttcg ccgttgtgat ttccggcaat accgcagtgc ctctcgacaa
      121 aatgctctca gaagagcagc ttaaattcgt tctctccgac acgggcgttt cgcttattat
      181 ttactcaaag cagtttgcgg atttcatccc cgcgtttaaa gacgagaacg agttgttgaa
      241 agtcttaagc atggaaaacg accttggctc ctgcctggaa aagggcggaa aggctttggc
      301 tgagaaaaat aaaaccgaag tcgaaatccg ccccgaaccc tccgacacag caatgataat
      361 atacacctcg ggcacaacgg gcattgcaaa gggagttatg ctttcccaca ataaccttac
      421 ctttgacgcg atgggtgttt caatgcacct tgaaagcggc ggcaacaacc ttttcgtgct
      481 gcctcttaat cacgcctacg ccatcatagc cattctcgtg gtaatgaaca agggaaagcc
      541 cacctgcata aactccggtc ttaaacgctt cgctcaggaa ataaaagagt ttaagcccca
      601 cgaaatgtat cttgtacccc tcatagttga aaatctttat gacaaaataa tggagaaggc
      661 aaaagcggaa ggtaaactaa aacagcttaa attccttatg aaactcagcc ttttcctcta
      721 ttcaatcggc atagacatca gaagaaagct cttcaagcca attctcaatg aattcggcgg
      781 caatctcagc attcttgtct gcggcggcgc cgcccttgac ccggattaca tcaaagcctt
      841 cagagcattc ggcataaatg tcctcatcgg atacggcatt tccgaatgtt cccccgtaat
      901 ctccgtcaac cgcaatgaac actggcgcga cggcagcgtg ggaattcccc ttaaaggcgt
      961 tgaggtcaaa accgacgctc ccgacggcaa aagcgaaggc gagctgtatg tccgcggcgg
     1021 catagttatg aagggctatt ggcgcaacga aaaggcaacc gccgaaacat ttgacggcga
     1081 ttggttcaaa acgggcgaca tcggctgcat cgacaaggac ggctttgtat atatcacagg
     1141 ccgcaaaaag aatgtcatca tcctcagcaa cggcaaaaac ctctatcccg aggagcttga
     1201 aatgcagctt gtgaaactcc ccgctattgc tgaagtcctc gtgtatgagg aaaacaaaaa
     1261 ggtcaccgcg gaaatcttcc ccgacgaaga ttttataaac gaaaacgata ttcaggatgt
     1321 taaggaatat atcgaatccg tggtggaaaa gtttaataag cagtttcccg cgtgcaagag
     1381 catcgcggcg gtaaagctta gaaatacctg ctttgagaaa acctccacca aaaaaatcat
     1441 aagaaagagg atagcctcat gaaagaaaga attctcaaaa tccttctgtc aaaccccaaa
     1501 atccagaatg tcacacccga aagcaatctt gtcaccgatt tcggtctttc ctcccttgac
     1561 cttgcggaaa ttgtctgcga tatagaggac gagtttgaca ttgaaatccc cgaaaaagac
     1621 ttagtaagaa tcaaaaccct tcaggacatc tacgactata tagacaccca cgcatagaga
     1681 aaaaggaaaa agatatgaag aagaaaaaag gtcattctcc gctttggtat tatgttgtcg
     1741 gcggaattat gggctactct aaaatcagag gccataatgt aaagttcaga atatcagatg
     1801 aagtaaagaa cctaaaacct ccgtttgtgg gcatagcaaa tcatcagggg tattacgact
     1861 gggcttatgc cgccagagca ttcttcccca tgcgctttcg ctttgtggtt tcaagatatc
     1921 agttcttaaa ccccaaaacg ggcaagctgc ttgaaaaact gaactctatc cccaaaagcc
     1981 agttcacctc cgacgcctcc gctgttaagg agattatccg caccataaaa agaggagaga
     2041 atgtgtttat cttcccctcc ggcagacttt cgcttttcgg cgaggacgac aagccagttg
     2101 aaggcactta tgagcttctt aaaaagctgg gcgtccccgt agtcatggtt caccttgacg
     2161 gcagctaccg cactgcgccc aggtacaata ccgaggtcaa aaagggcagg gtagtggtca
     2221 ccgcctatgt gctgttcact cccgaggaat taaagaatct ccccgaggag gaggcaaaga
     2281 aaaggctcta cagcgccttc tgccacgatg acttcaattc cgaaaatgta tgcgaattca
     2341 aaagccccga tatagcaaag ggtcttgacg accttctcta tatgtgcccc gactgcaaag
     2401 cgaattacac tgtaaaagta atgccgggtc agaaactgat gtgcaaagac tgcggcttta
     2461 gtgtgcagat ggacaattac tttaagttta ctgccctcag cggtaacgca tcgcctaaaa
     2521 ccattacaga gtggtcgcat ataataaagg ctgaggaaaa aaagcgtata ctttctgacc
     2581 cggactttac tttaagagaa aaaatggatg tattggagca cattaaagaa gacgaacagc
     2641 tcactaaaac cggggaatgt gaaatcagct tgagccgtga cggcattaag atagaaggca
     2701 caaaaaaaga cggcagattc agccgccatt acaccctaaa agagtacccc gcaatgcaca
     2761 tattcgataa gatttatctt ctcgtgcccg acaatgaggc ggttatctgc gtatcgcccg
     2821 cgaacgccaa aatcgccaca cgatgggcag tcgtatcgga gacactgtca tcactcctct
     2881 tataaccgta catttgctcc tctttttcat tgttgcgcag aaaggcatcg gcttattgcc
     2941 gatgcctttc tgttttttat taagatttgt tttattcgtt atccgctaag acatccgtct
     3001 tttcaattac gggctggtcc tcggtttcct ctttgccggg gaagttttcg taaacctcat
     3061 aaacattttc aaacagagct atttccctga ctatattgtt aaagtttacg gctttcggca
     3121 ctttaatcat atatctgcag cgggaaattc tttcgcccgc ggagttaaaa tggtcgcccg
     3181 agagcagata ctcaaaggaa aggaccgtaa tcttctttct ctcaaagtaa tcgtcaagct
     3241 gaggaattgt ggttctctta tctgttatgt aaagctcaag tctgcgggtt ctgagacgag
     3301 caaagaatct tctctcaacg cttttaagaa taactgttgt tccgaatact gcgaggaaaa
     3361 cgatgaaagc cattctgaaa tatcccatgc ccacagcaag acccatgcag gcggttgccc
     3421 aaagggtcgc cgcggtggta atgcccgtaa ccatcgtacc gtctctggat ttcatgatgg
     3481 cgccggcgcc tatgaagccc atgccggaaa caacgcccga cgcgattctt gaaaggtcta
     3541 ctcttgcgcc ggggtaaagg taaatcgaaa gtcccatctc cgccgcgatt atggatgcaa
     3601 gcgcagaacc aaggcagaca agaacatgtg ttttcattcc agcgggatga ttcgaaatct
     3661 ccctttcaat gccgataacc agtcctattg caatagccgc aaggacgcga atggttatat
     3721 cgggccaagt caaaatctga gtatattcaa ccattgcaat tctccttaat nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tatatcgggc caagtcaaaa tctgagtata
     3901 ttcaaccatt gcaattctcc ttaatatgta atctaaaggt taatcccgtt tgttgttaaa
     3961 tattgcaagt ccggagccga gtacggtcca taaaagctgg agcagtatta tacctatgtt
     4021 tcccgctttg gagatatatc ctgcaattgc cataaagaga acaattacgg ctcccgcgat
     4081 gccgcccgcc gcaaagaggg gaatcagcat cttgccggtt tttgcaagct tcaaagccga
     4141 tgcaaatgcg gagaacaagg agagagctcc ctgtttgtga attaccgacg cggggacctc
     4201 ctttgacctt tcgttctcta tgcattcctt aaagaactcc gcggaggcgg aagtaagaac
     4261 tctgactccg tttttgcccg tgccgaagct gctttctata aattcctctg tgatattgga
     4321 atctctggat gttacaagca ccgtaattcc ctgattcaca agggcgttaa gccccttctt
     4381 gaccgccgcg gaggggtagt agcttacaac cagcatcgcc gcgagctgcc cttcaaccga
     4441 gaggtaaatt acccgcttgc cgtcctccgt gtatttcttt tcaaaactgt cgtcgggcgc
     4501 cttaacgctg tgatttctga gaagctgagc gttgcccaca agaattctct tttcgtttat
     4561 ccatgcggaa aggccgagtc tgtcctcata ggacagcgcc tcaacgggca aaagcagctt
     4621 gtcgttgccc attatgacct tttcgaacgc gggctttaat gggccgcccg ccttgatgac
     4681 tgtggaggct atgtataaaa tcgccttatc cagcttcatt ccgccaaagg tttttatgcc
     4741 gtgtatctcg caggcatccg gcgggaaaag ctcgttcgcg tcgattatga aggtatctgt
     4801 ttcagtaagg tttagcgccg ccttaaaacc tgtcacatag catttcgccg cagttttaag
     4861 gttctcgagc agaagcccga gtgacgacgc gggcaaaact ccgaccggga agctgattat
     4921 cgccagagcc gcgagagcga aaaatccttc aaaaacatcg cccttaaatg ttgcggctat
     4981 aaacgccaca acagcctccg cgactatcag cgcaaaaccc ctggagagaa tctttgccga
     5041 aaggctgtcg cccatggact cagaaataaa tgaggaaata aactcaggct ttgccgagta
     5101 tttgatgcgg gggttttcca aaggcaaatg cctttttata tccgcatacg cgtcggaagc
     5161 tccaaggtcc gccacggcgc acatttcatc ctcgttctta acgaggaaag caaagcccct
     5221 ctttatgttc aaggcgttta tgtatttgcc cgcgaaaact attgagcaga gtataagcgc
     5281 agagggggcg agaagactta aatcgtgcgc caaagactca tagccgaaaa gcattacaag
     5341 gctttgaatc gcagccgcaa agagcatcag cattacgccg gaatttgcgt cgggctgctt
     5401 tttaacggcg gagttaaggc cgagtctaat ctcgggcaga gccacaaaga aggctcccgc
     5461 aaggattata agctgcaaga ttatgcttgc cagtaatctg tttttaagct cagacgccaa
     5521 gggctcgccg accttataaa acagcggcaa aagaatcgat acaatgccga gagcgaaaaa
     5581 cagcgcagcc tttgcgccct gcttgctctt gtaaaggttt aaaaagccag cgattctctt
     5641 tttatccttc ggatttctgt attcgggata tattccgagt gaggctgacg gcatttccag
     5701 cactatgccc tctgcctttt tttctttaga cttttccttt gaacgaggcg ctttggtttt
     5761 ttgagttttc ttcttttcgg ttatttcttc aatatccgcg tcatcagata cagccttttt
     5821 ggggactttc ttttcttctg acgaaatgtc gtcccacttg ttttcgccgt agagattgaa
     5881 gatcttagcc tgttctattc tgctttttct gagagctgcc tcgatttttc tttcctcaag
     5941 cctgtcgctg ctgtctatag gctcaaagcc cgagaaaaag agctgctgag gaatttcctc
     6001 gtcctgctca atttcacccg cctccaaggc gcccgattcc gcaatagcct taatgcgggc
     6061 tatctgtctc tttttcgtgt ccgctctgtc gagggtatcc cacagctcat cgtggaaaac
     6121 cattgtatgg gcagggtcgc tctccgttgt gcggataaac ctctgccttt tctgcggcat
     6181 ttcctcggtc actgcggtaa aagcctttgt cttttccaaa tcgagtttac tcagttcatc
     6241 ggatgtaatg gtgccggg
//
LOCUS       DAONVQ010000113         6255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9359_length_6255_cov_4.762097, whole genome shotgun sequence.
ACCESSION   DAONVQ010000113 DAONVQ010000000
VERSION     DAONVQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9359_length_6255_cov_4.762097"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLD98_07325"
     CDS             complement(<1..769)
                     /locus_tag="PLD98_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HQA06088.1"
                     /translation="MYKFTGFTEKANTALNHAVQIAEDLGHTYIGSEHILIGLAKEGG
                     TIASKVLSDRRITADVLIEQVKKSVGVGIPTSLTPNDFTPRSKNIIELAASAARSMGL
                     SLVGTEHLLLAIVHEGSSFAANLLVQLGVSPNDIINDINKAMMGSAPKDGISGRQSSS
                     GSDFRKSGQSTPTLDQFGRDLTVLAKEGAIDPVIGRQKEIERVIQILSRRTKNNPVLI
                     GEPGVGKTAIAEGLALKIVSGEVPELLKDKRIVALDLT"
     gene            complement(791..1819)
                     /locus_tag="PLD98_07330"
     CDS             complement(791..1819)
                     /locus_tag="PLD98_07330"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HQA06089.1"
                     /translation="MADKRWYSNTGKDGDVVLSTRIRLARNLADYPFPHRLDTAGKEK
                     VGREVRDAVKDVPALHLRFIEMKDLTKTNAVALAERHLISPEFASSSEGRSLLLSDDE
                     SVSIMICEEDHIRLQVILGGLALEEAYEKASLIDDILDERLKYAFNENFGYLTQCPTN
                     LGTGMRASVMLHLPALSKLAKIQKIANAVSKLGLTVRGAYGEGSSPGGDIYQLSNQVS
                     LGISEQEALDNLKSITLQLATQERAARKELLKDINIQDKVSRAYGILLTSRVLSSKEL
                     MELLSWVRLGASENLVPVKTELINEMFVTMQPANLIESIHSDADPATRDEIRAERVRK
                     ALEAGRIN"
     gene            complement(1809..2315)
                     /locus_tag="PLD98_07335"
     CDS             complement(1809..2315)
                     /locus_tag="PLD98_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HQA06090.1"
                     /translation="MLCQHCKKVEATTHITQIINGETAEMHLCQNCAQELGYNDMFTN
                     FSFGLSELFGSYFADAPVSAISNRVIRCEKCGSTFNDIARSGKIGCQDCYTLFYDRLM
                     PSIRRMHGKTHHEGKLPKGANEEVKRARRISDLKKRLNKAVDEQNFELAAQLRDEIRE
                     LEAGNDGR"
     gene            complement(2316..2780)
                     /locus_tag="PLD98_07340"
     CDS             complement(2316..2780)
                     /locus_tag="PLD98_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HQA06091.1"
                     /translation="MNMSNMIYRILLDMLEAEGIAEIQRNELAEQLGCVPSQINYVLS
                     SRFTPEHGYIVESRRGGKGYIKITRVEATDNEIIMHLINSIGDEIDEGSARSHINNLV
                     HNYIISKQYGDLLLAAVNENAYKGIPKNLRHNARAGVFKQLLLSMVDLKGGV"
     gene            complement(3078..3704)
                     /gene="tmk"
                     /locus_tag="PLD98_07345"
     CDS             complement(3078..3704)
                     /gene="tmk"
                     /locus_tag="PLD98_07345"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQA06092.1"
                     /translation="MTGKFIVFDGLDGTGKTTFIKMLSEYLESKGFKVSVTAEPTKGP
                     SGVLIREILGGAKKASQEELAALFLADRIAHNSELDELINEGNIILCDRYYYSSFAYQ
                     ADSDTMGWVMDMNLNCPKIRVPDLALLFDSPPELCLERINKGRMGQPLEIFENLKSLN
                     AIREKFALIEKILNESGRNENIVRIDSAPKLEVVFEEILKAVNSLLGI"
     gene            complement(3707..4585)
                     /gene="rsmA"
                     /locus_tag="PLD98_07350"
     CDS             complement(3707..4585)
                     /gene="rsmA"
                     /locus_tag="PLD98_07350"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQA06093.1"
                     /translation="MAELTDLSYIKSLLSSHGFTFSKALGQNFLINPAVCPKMAELSG
                     LHPDAGALEIGPGIGVLTKELAKRSKHTVAIELDKRLLPVLDETLKDFSNISIINDDV
                     LKADLQRIIENEFYSRGINEIYVCANLPYYITSPVIMRLLESELPFKALTFMVQKEAA
                     DRLCAAVGSRDSGAVTVAVAVRAKAEKLFSVKAGSFFPAPKVDSAVIKLTPYETPPLA
                     VESMKFFTSFVRAAFEQRRKTLSNSVSSALGFDKSDIIEILKSCGISEAVRAEALSLE
                     DLARISNALYKLKIKE"
     gene            complement(4597..5586)
                     /locus_tag="PLD98_07355"
     CDS             complement(4597..5586)
                     /locus_tag="PLD98_07355"
                     /inference="COORDINATES: protein motif:HMM:NF005304.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA06094.1"
                     /translation="MAYSENVYTKAKEELDRRRRKAETELSERRSELILRFPELKDLL
                     REMARAGYEAVKVIGMGADAAEYIEKLKKRNLEIQDDIKTILKSANLPLDYLEPHYTC
                     ENCEDTGTAGGRICSCYKELLKKYATEELCASSPLKLSSFEDFSLDYYPDTPLNGDGM
                     TVRKQMGYIFDYCKKYAEDFGRDSVSLLMYGETGLGKTHLSLAIGGEAIKKGYGVVYG
                     SAHNLLSRLENERFGRTADKSFEAEKTLLECDLLIIDDLGTEFSTQFTVAEIYNIINT
                     RLISGLPTIINTNLSIAKLEEKYSPRVASRIIGEYQLLRFCGRDIRQLKRNSL"
     gene            complement(5586..>6255)
                     /locus_tag="PLD98_07360"
     CDS             complement(5586..>6255)
                     /locus_tag="PLD98_07360"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HQA06095.1"
                     /translation="LPVLKPTQEQIAMRVEESEEIRALLNEAQQILGRTIGYSAQAAL
                     VMLHDHYALPVSVILMICEYARSTGRQGNINYIQTMGKNWAERGIDTFEKAVEQLAAL
                     ENTDKLWKEFAAFAGITSPRPTTTQQRYLDTWHNKWKFPLEMICIAYEKAAERTGQLN
                     MNYMNGIIKNWYESGVKTVEDIEKLDKIPKVTKLSDAKTSYDIEQAEKSAFEPPIYTK
                     RGAK"
BASE COUNT         1367 a         1531 c         1498 g         1859 t
ORIGIN      
        1 ctgtaaggtc aagcgcgacg attcgcttat cctttaaaag ttcgggtact tctccggaaa
       61 caatcttcag cgcaaggccc tcggcgattg cggtcttgcc cacgcccggc tcgcctatca
      121 gaacgggatt gttctttgtg cggcggctga gaatctggat aaccctttca atctcctttt
      181 gtctgccgat aacggggtcg atggcgcctt ctttagccag aacggtcaaa tccctgccga
      241 actggtcaag ggtgggcgtc gactgaccag actttctgaa gtctgagcct gaagagctct
      301 gcctgccgct gatgccgtcc ttgggagctg agcccatcat tgccttgttg atgtcgttta
      361 taatgtcgtt aggcgatact cccagttgaa caaggaggtt tgcggcaaag ctgcttccct
      421 cgtgaactat ggcaaggagc agatgctcgg tgcccacaag ggagagcccc attgaccttg
      481 cggctgaggc tgcaagctct atgatattct tgctcctcgg ggtaaagtca ttgggagtga
      541 gcgaggtggg aatgcccacg cccacgctct ttttaacctg ctctatcaaa acatccgctg
      601 ttattctgcg gtccgaaagc actttggagg caatggtgcc gccctctttc gcaagaccta
      661 tgagaatgtg ctcgcttcct atatatgtgt gtcccaaatc ctcggctatc tgaacggcgt
      721 ggtttaaggc tgtgttagct ttttcggtaa agcctgtgaa tttgtacata tgaccaagtc
      781 ctttctgcct ttagtttatc ctgcctgcct caagggcttt tcttaccctt tccgccctta
      841 tttcgtccct tgttgcgggg tctgcgtcgc tgtgaatgga ttcaatgaga tttgcaggct
      901 gcattgttac aaacatttca tttataagtt ctgtttttac gggcaccaag ttttcggagg
      961 ctccaaggcg tacccatgac aaaagctcca taagctcctt gctgctgaga acccttgatg
     1021 taagcaatat accgtatgct ctgctgacct tgtcttgaat gttgatgtcc tttaaaagct
     1081 cttttcttgc cgctctctcc tgagttgcga gctgcaaagt gatggattta aggttgtcga
     1141 gagcctcctg ctcggaaatg cccagagata cctgattgct gagctgatat atgtcgccgc
     1201 cgggactgga gccctctccg tacgcgcccc tgacagtcag acccagctta gatacggcat
     1261 ttgcgatttt ttgaatcttg gcaagctttg aaagggcggg cagatggagc attacggatg
     1321 ccctcatgcc tgtgccaagg tttgtggggc actgagtgag atatccgaag ttttcgttaa
     1381 aggcgtattt taatctctcg tcaagtatgt cgtctataag ggaggctttt tcatatgctt
     1441 cttcaagggc aagaccgccc agaatgacct ggagcctgat atggtcctct tcgcaaatca
     1501 taatgctgac cgactcgtca tctgaaagaa gcagggacct tccctcggat gagcttgcga
     1561 attcggggct gattaaatgc ctttcggcaa gagctaccgc gttggttttg gtaaggtcct
     1621 tcatttcgat gaacctgaga tgcagcgcgg gcacatcctt aacggcgtct cggacctctc
     1681 tgcctacctt ttccttgccc gcggtgtcaa ggcggtgagg gaagggatag tcggcaagat
     1741 ttctggcaag ccttatcctt gtgctgagaa ccacatcgcc gtctttgccg gtgttggaat
     1801 accatctctt atctgccatc gttaccagcc tccaattctc tgatttcgtc cctcagctgc
     1861 gcggcaagct caaagttctg ctcatccacc gctttgttaa gcctcttttt aaggtctgaa
     1921 attcttcttg ccctcttaac ctcttcatta gctcctttag gcagcttgcc ctcgtggtgg
     1981 gtcttgccgt gcattcttct gattgagggc atgagcctgt cgtaaaagag agtgtagcag
     2041 tcctgacagc cgattttgcc gctgcgggct atgtcgttaa aggtggagcc gcatttttcg
     2101 cagcggatta cccgattgct tatggcgctg acgggcgcgt cggcaaagta gctgccgaaa
     2161 agctcggaca aaccaaagct gaagtttgtg aacatatcgt tatatccaag ttcctgagcg
     2221 cagttctggc aaaggtgcat ttcggccgtt tcgccgttga ttatctgcgt aatgtgagtt
     2281 gttgcctcta ccttcttgca atgttgacaa agcatttaaa ctcccccttt taaatctacc
     2341 atagaaagca aaagctgttt gaaaaccccc gctcttgcgt tgtgtctcaa gtttttggga
     2401 atgcccttgt aggcgttctc gttcactgcc gcgagcaaaa ggtcgccgta ctgcttgctg
     2461 ataatataat tatgaaccaa attattgata tgactccttg cggagccctc gtcgatttca
     2521 tcgcctattg aatttataag gtgcattatg atttcgttgt cggttgcctc gactcgggtg
     2581 attttgatgt aacccttgcc acccctgcgg ctttctacta tatagccgtg ctccggtgta
     2641 aagcgtgagc tgagaacata attaatctgg ctgggcacgc agccaagctg ttccgccagc
     2701 tcgttgcgct ggatttccgc tatgccctcc gcttctagca tatcaaggag gattctgtat
     2761 atcatattgc tcatattcat tagtctcatc cccttccttt aacttctctc taccccttac
     2821 atatccacta aaatatttga caaaatgccg taaaataatc aggaaaagac atttttgact
     2881 ttgactatct tttacctttg tttacaatat atccttttaa cgcaaaaagg tcaaaggtca
     2941 ataaaggtca aatagggctt ttacttttat ttatctgccg ttttcttttg tttgctttta
     3001 ttttctttta atttctttaa aattctaact tttgaccaaa caaaaagctg ctgccattgg
     3061 cagcagctaa tcttctttta tataccgagt agagagttta ctgctttgag aatttcctca
     3121 aacactactt caagcttcgg ggctgagtca atgcggacta tgttttcgtt cctgccgctc
     3181 tcattgagga ttttttcgat aagagcgaat ttttctctga tggcgtttag ggatttaaga
     3241 ttttcaaaaa tctcaagggg ctgacccatt ctgcccttgt tgattctttc aaggcaaagc
     3301 tcggggggag aatcaaacaa aagggcaagg tcgggaactc ttattttcgg gcagttgagg
     3361 ttcatatcca taacccagcc cattgtatcg ctgtccgcct gatacgcaaa ggaggaataa
     3421 taatacctgt cgcagaggat tatattgcct tcattaatca gctcatcgag ttcgctgttg
     3481 tgggctattc tgtcggcaag aaagagagcc gcaagctcct cctgggatgc ctttttggcg
     3541 ccgccgagga tttcacgaat caaaacccct gagggcccct ttgtgggctc cgcggtgacg
     3601 gacaccttaa agcccttact ttcaaggtat tcggaaagca tttttataaa tgtggttttg
     3661 cccgttccgt caaggccgtc aaaaactatg aatttgccgg tcataattac tcctttattt
     3721 taagcttata aagcgcgttc gatatgcgcg ccaaatcttc aaggctcaga gcctcggctc
     3781 tgacggcttc gctaatgccg caagatttta agatttctat aatatccgat ttgtcaaagc
     3841 ccagcgcgga tgacacggaa ttggaaagcg tctttcttct ctgctcaaag gcggctctga
     3901 caaatgaagt gaaaaacttc atgctctcaa cagctaaggg aggggtttca tagggcgtga
     3961 gcttaattac cgcgctgtca actttggggg cggggaaaaa tgagcctgcc ttaacgctaa
     4021 agagcttttc cgctttggct ctgacagcaa cggctacggt gacggctccc gaatccctgc
     4081 tgcccaccgc agcgcagagc ctgtccgcgg cttctttttg caccatgaag gtcagagcct
     4141 taaagggcag ctcagactca agaaggcgca ttattacggg ggaggttata tagtaaggga
     4201 gattcgcgca gacatagatt tcgttaatac ctcttgagta aaactcgttt tcgattattc
     4261 tttgaaggtc ggccttcaga acatcatcgt taattatgct gatgtttgag aaatccttta
     4321 gagtttcatc aagtacgggc aatagccttt tgtcaagctc aatcgccaca gtgtgctttg
     4381 acctttttgc aagctctttt gtcagaactc caatgccggg tccaatctcc aatgcgcccg
     4441 cgtccgggtg aagacccgac agctcagcca ttttggggca gacggcgggg ttaataagaa
     4501 agttttgccc cagagctttt gaaaaggtaa aaccgtggct gcttagcagc gactttatat
     4561 aggacaggtc cgttaattct gccattttgc ctccgattac aaggaatttc ttttaagctg
     4621 ccttatgtcc cgaccgcaga atcttaacag ctggtactcg cctattatcc ttgacgctac
     4681 gcggggggag tatttttcct caagttttgc gatagagaga tttgtgttta ttattgttgg
     4741 cagccctgaa atcagccttg tgttgatgat attgtaaatc tcagctacgg tgaactgggt
     4801 ggaaaattct gtgcccaaat cgtctataat caacagatcg cactcaagta gagttttctc
     4861 cgcctcaaag gatttatccg ctgttctgcc gaatctctcg ttttcaaggc gggacaagag
     4921 attatgggct gagccgtaaa ccacgccgta gcccttcttt atagcctcgc cgccgatcgc
     4981 gagggagaga tgggttttgc caaggcccgt ttcgccgtac ataagaaggc tcacagagtc
     5041 cctgccgaaa tcctcggcgt attttttgca gtagtcgaag atatatccca tctgctttct
     5101 gacggtcatt ccgtcgccgt ttaagggcgt gtcggggtag taatcgaggg agaaatcctc
     5161 aaagctcgaa agctttaaag gcgatgaggc gcaaagctcc tctgtggcgt actttttaag
     5221 gagttccttg taacaggagc agattctgcc gcccgcagtg ccagtatcct cgcaattttc
     5281 gcaggtataa tgtggctcaa ggtaatcaag gggcagattt gccgacttta agatggtctt
     5341 tatatcatcc tgaatttcaa ggtttctttt tttcagcttt tctatgtact cggcggcgtc
     5401 ggcgcccatg ccgatgacct ttacagcctc atagcctgcc cttgccattt ctcttaggag
     5461 gtctttaagt tcggggaagc gaagaataag ctcggagcgg cgctcgctaa gctctgtttc
     5521 agcctttctg cgccgtctgt ccagctcttc ttttgccttt gtatagacat tttcgctgta
     5581 tgccattact tagcacccct tttcgtgtaa atgggaggct cgaatgccga cttttccgcc
     5641 tgctcgatat cgtatgaggt cttagcgtcg ctgagctttg ttaccttagg aattttgtcc
     5701 agcttttcta tatcctcaac ggtcttaacg ccgctttcgt accagttctt tataatgccg
     5761 ttcatgtagt tcatattgag ctgacctgtg cgctcggcag ccttttcata ggctatgcag
     5821 atcatctcaa gtgggaattt ccacttgttg tgccatgtgt caagatacct ttgctgggtg
     5881 gttgttgggc gggggctggt aatgcccgca aaggcggcaa attccttcca cagcttatct
     5941 gtgttctcaa gggcggcaag ctgctctaca gccttttcga aggtgtctat gccccgttca
     6001 gcccagttct tgcccattgt ctgtatgtag tttatattgc cctgtctgcc cgttgacctt
     6061 gcgtactcgc atatcataag gattactgaa acgggcaagg cgtagtggtc atgaagcata
     6121 acgagagccg cctgcgctga gtagccgatt gttctgccga gtatctgctg ggcttcgtta
     6181 agcagcgccc ttatctcctc cgactcctct accctcatgg ctatctgctc ttgtgtgggt
     6241 ttcagaacgg gcagg
//
LOCUS       DAONVQ010000114         6245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9386_length_6245_cov_3.560582, whole genome shotgun sequence.
ACCESSION   DAONVQ010000114 DAONVQ010000000
VERSION     DAONVQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9386_length_6245_cov_3.560582"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..701)
                     /gene="aroQ"
                     /locus_tag="PLD98_07365"
     CDS             complement(267..701)
                     /gene="aroQ"
                     /locus_tag="PLD98_07365"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQA06096.1"
                     /translation="MKLLFINGPNLNMLGIREPEIYGSKTYADLVEFINGFCKELGIE
                     AEIFQSNHEGAIVDKIQEALGKFDGIVINPAAYTHTSVAILDAVKAVGLPTVEVHISD
                     VSKREGFRQVSYIREACIATISGQGFEGYKTAALTLLKELNK"
     gene            complement(771..2951)
                     /locus_tag="PLD98_07370"
     CDS             complement(771..2951)
                     /locus_tag="PLD98_07370"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQA06097.1"
                     /translation="MFEEFRKFETTLAGRPLVIETGKMAQLASGSCLVRYGETEVLCT
                     ATMADKPREGIDFFPLSVDYEEKLYAVGKIPGSFQRREGRASEKATLASRLIDRPIRP
                     LFPKDMRNDVGIIATVMSVDPDCSPEITAMIGVSVALSISEIPWDGPIAGVAVGLVDG
                     EIVINPTEAQREVSDLTLTVASTDSLVAMIEAAANEVPEDVMLKAIMKAHEVNQELVE
                     FIKGIQKEIGKPKVDFPSNDPPAEMFEAIKEFAIDDIKFALDTNDKRIREERLKPVYE
                     KVHEKFDELYPEETVKIDDCLYLLQKYVVRRWLLDEGKRVDGRGMDEIRPLAAEIDVL
                     SRVHGSALFTRGQTQVLTVTTLGTASDAQNLDGLDNEEIKRYMHHYNFPSFSVGETKP
                     PRGPGRREIGHGALAEKALLPVIPPVEEFPYTIRLVSEVLSSNGSTSQGAICSSTLSL
                     MAAGVPIKAPVAGISCGLVTEGDRYITMVDIQGVEDFFGDMDFKVGGTKKGITAIQMD
                     IKIHGLTFEIIEEALEKTRKARLYILDEVMLPVISEPRKELSKYAPKMLLTKIDIDKI
                     GDVIGKQGKVIQKICSECNCKVDVEEDGTIYITAIDIDDARRALNMIETIANDPEVGA
                     IYKGVVTKIMSFGAFVEIAPGKEGLVHISHLDVKRTEKVEDVVHVGDEIIVQVLGIDE
                     QDRINLSRRNALIEVEGLTPENTPAPRRPAPPRHGGNKPNNRRR"
     gene            complement(3171..3434)
                     /gene="rpsO"
                     /locus_tag="PLD98_07375"
     CDS             complement(3171..3434)
                     /gene="rpsO"
                     /locus_tag="PLD98_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQA06098.1"
                     /translation="MTQEEKQAIMKEYGLHEGDTGSPEVQIALLTYRINNLTEHLKTH
                     KKDHHSRRGLLKMVGHRRNLLAYLQKKDIERYRSIIAKLGIRK"
     gene            3859..4821
                     /locus_tag="PLD98_07380"
     CDS             3859..4821
                     /locus_tag="PLD98_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HQA06099.1"
                     /translation="MKLFSVKPSLKEIIWGGKKLMELYPDSVSMANIAEAWVLSGHKD
                     GHSVVRNGEYAGRTLEETIKSMGPECLGTKAEGLTEFPLLIKLIDAKDKLSVQVHPDN
                     AYAREFENSNGKTEAWFVLDAEEDACIVYGFNRDITKEEFKDRIKNGSLSEVLNTVKV
                     KRGDVIYLPSGTVHAIGSGILLAEVQQSSNLTYRVYDYDRIDKNGCKRELHIDKAVEV
                     ANTAKAEANFEPEGERSEICGGCKTLLKKSEYFTMELIEVESRTEEYATEESFTSFLV
                     LEGSGAVGCGGEVMPVKRGDSIFIPACKDKIALAGRLKLLKTTL"
     gene            4950..6125
                     /locus_tag="PLD98_07385"
     CDS             4950..6125
                     /locus_tag="PLD98_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQA06100.1"
                     /translation="MKKSTKRILSLLLVAAMSVVMVFSSFAADAIDVTQPTDIQLQFN
                     EDGKFRIIQFADCQDIALHSPLMVNFIEKTLEVYKPDLVVFTGDNVVQPLAPLNKMAI
                     EAIIDPVAKAGIPFTFTFGNHDAESISKERMYQVYRSYPNCIAYDADPDLSGMGTCNH
                     TILSSDGSRIAFNIWIFDSNMYEYSTGKREYDFVHEDQIEWYKRTSEALEAQAGYKVP
                     SLAFQHIPVPEIYELYLEAQPGDSPTKEYNGKTYALKLNPEMATGYLGEFSCPPVVNS
                     GQFDAFVERGDLLGIAFGHDHINSFVGTYKGIDLIQTAGVSMHSYGDDAVRGARLFVL
                     DENGTYETEAFTYNDLFTEAEFIALKMAQVPAYLVQFAKVFLYLLQYIFSIYPGIKL"
BASE COUNT         1613 a         1478 c         1436 g         1718 t
ORIGIN      
        1 cccagcttaa cggggatatt aagctcctcg ccgtactcgt atttgccgtc gcctaagtat
       61 ttgccctctt taacaaatac ccagttttca atgttaaagg attcaaaatc cctgtatttt
      121 ttcagctggc gcatcataaa ctctaaaacc atcggtttgg gagttttcat ataaaacctt
      181 ctttccatgc ggtttcgtta atgatagcat agcaaaacgg caaagacaat agcacagcta
      241 cgcctttgcc gttaaactcg gatttgttat ttattaagct ccttgagcag cgtcagggct
      301 gcggttttat agccctcaaa gccctgaccc gatattgtgg ctatgcacgc ttctctgata
      361 taagaaacct gtctgaagcc ttcccttttt gaaacatcgg agatatgtac ctcaaccgtc
      421 ggcagcccta ccgcttttac tgcgtctaaa atagcaacgc tcgtatgcgt ataggccgcg
      481 ggatttatca caatgccgtc gaattttccg agagcctcct gtattttatc cacaatcgct
      541 ccctcatggt tggactgaaa aatctcagcc tcaattccca gctccttaca gaatccgtta
      601 ataaactcca caaggtccgc ataggttttg ctgccgtaaa tctcgggctc tcttattccc
      661 agcatattga ggtttggacc gttaataaaa agcagcttca tatcaaaacc ccttttaact
      721 ttttgcaaat gcagtcccct gaaaatcagg agactgcata tcattttata ttatctgcgg
      781 cggttgttgg gcttatttcc accgtgcctc ggaggggcgg ggcggcgggg cgcgggggtg
      841 ttctcgggag tcagaccctc aacctcaata agagcgtttc ttcttgagag attgattctg
      901 tcttgctcgt cgatgccgag aacctgaacg ataatctcat cgcccacatg aacaacatcc
      961 tcaacctttt cggtgcgctt tacatcaagg tggctgatgt gaacaagacc ttccttgccg
     1021 ggagcaattt caacgaatgc gccgaagctc ataatctttg taacaacgcc cttgtagatt
     1081 gcgcccacct cggggtcgtt ggcaatggtc tcaatcatgt tgagagcgcg tcttgcgtcg
     1141 tcaatatcaa tagcggtgat ataaatagta ccgtcttcct caacatcgac tttgcagttg
     1201 cactcggagc aaatcttctg aatgaccttg ccctgttttc cgataacatc gccgattttg
     1261 tcaatgtcaa tcttggtaag gagcattttg ggagcgtatt ttgaaagctc ttttctcggc
     1321 tcggagataa cgggcagcat gacctcgtca agaatataga gtcttgcctt ccttgtcttt
     1381 tcaagagctt cctcgattat ctcaaaggta agaccgtgaa tcttaatatc catctgaatg
     1441 gcggtaatgc ccttctttgt gccgcctacc ttaaagtcca tgtcgccgaa gaagtcctca
     1501 acgccctgaa tgtccaccat tgtgatatag cggtcgccct cggtcacaag tccgcaggaa
     1561 atgcccgcta cgggagcctt aatcggaacg ccggccgcca taagcgacag agtggatgag
     1621 cagattgcgc cctgggatgt ggagccgttg gatgagagaa cctctgaaac aagtcttatg
     1681 gtgtagggga actcctcaac gggcgggatt acgggcagga gagccttctc cgcaagcgcg
     1741 ccgtgaccga tttcccttct tccgggacct ctgggaggct tggtttcgcc gacggagaat
     1801 gaggggaagt tgtagtggtg catatatctc ttgatttcct cattgtcaag tccgtcaagg
     1861 ttctgagcat cgcttgcggt gccgagtgtt gtaactgtca atacctgcgt ctgacctctt
     1921 gtgaagagtg ctgagccgtg aactcggctg agaacatcaa tttcagcggc gagcggacgg
     1981 atttcgtcca tgcctctgcc gtctacgcgc ttgccctcat caaggagcca gcgcctgaca
     2041 acatacttct gcagcagata cagacagtcg tctatcttta cggtctcctc agggtacagc
     2101 tcgtcaaact tttcgtgaac cttttcgtaa acgggtttga gtctctcttc tcttatgcgc
     2161 ttgtcgttag tgtcaagggc gaatttaatg tcgtcaattg caaattcctt gattgcctca
     2221 aacatttcgg cgggcgggtc gttggacggg aagtctacct tcggctttcc gatttccttc
     2281 tgaatgcctt taataaactc aaccagttct tggttgactt catgtgcctt catgattgcc
     2341 ttcagcatta catcctcggg cacttcgttt gccgctgcct caatcatggc tacaaggctg
     2401 tcggtggagg cgacggtcag agtaaggtct gaaacctctc tctgagcctc tgtggggttg
     2461 atgacgattt cgccgtcaac aagacctacg gcaacgccag cgatggggcc gtcccacgga
     2521 atctccgaaa tgctcagagc cacggaaacg cctatcattg cggtaatctc gggtgagcag
     2581 tcggggtcaa ccgacataac ggttgcgata atgcccacat cgtttctcat atctttgggg
     2641 aaaagaggtc taatcggtct gtcaataagt cttgaagcaa gggtagcctt ttcgcttgcc
     2701 ctgccttctc ttctctggaa agagccgggg attttgccta cggcataaag cttttcttca
     2761 tagtcaacag acagggggaa gaagtctatg ccttccctgg gtttgtccgc catggtggcg
     2821 gtgcagagaa cttcggtttc accgtacctt acaaggcacg aaccgctggc cagctgagcc
     2881 atcttgccag tttcgataac aagcggtctg ccggcaagtg ttgtctcaaa ttttctgaac
     2941 tcttcgaaca tttcactacc tctgaaatat atatttccaa ggcaagcact cgtttagcaa
     3001 taaatacagt gattccgcat gggccaaatc actctattca ctgctaaccc gcgtgcttta
     3061 cctttggatt ctaaattatt atagccatta cggcaaaaaa aatgttataa gtatttgtat
     3121 aaattaaatt tagggcgatg gactgaaata gcccccgccc taattggttg ttatttacgg
     3181 atgccgagct ttgcaataat agaacggtat ctttcgatat ctttcttttg cagataagca
     3241 agaaggttgc gcctgtgacc taccatttta agaagtccgc ggcgggagtg gtggtccttc
     3301 ttgtgtgtct tgagatgctc tgtaagattg ttgattctgt aagttaaaag agcaatctgt
     3361 acttccggtg aaccggtgtc gccttcatga aggccgtatt ccttcattat ggcctgtttt
     3421 tcttcttgtg tcatactttt tcacctcatt aagatttttc cgagacccca gagcaaggca
     3481 ggtgaataac tcaaagagag tttaatccat gcaccgagat tctggaagct gtctgaccgc
     3541 agccgacagc tgttgtatta tagcacagcg gaaattaaat gtaaagtcct tttatattta
     3601 tttgtcttca attatgtggc ttatatatgt cttctgattt atgttttgca tttgaaatgc
     3661 agttattcta atacggaaat taattcgtca aaaatcgaca aaatttttga atttagataa
     3721 aattttagcg aatttattaa tatttacaga tttgtaaaca aaatggttag ttttttgtta
     3781 aattgtgtgc aaaaatagtt tgaaaaattg tcggatatgt gataacatat tcaggaattt
     3841 cacttgaggt gttgtcaaat gaagctgttt tctgtcaaac cgtcattgaa agagataatt
     3901 tggggcggca aaaagctcat ggaattgtat cctgatagcg tctctatggc gaatattgcc
     3961 gaagcatggg tactgtccgg ccacaaggac ggacatagcg ttgtccgcaa cggcgagtac
     4021 gcaggcagaa cccttgagga gacaataaag tcaatgggcc ccgagtgtct cggcacaaag
     4081 gcggagggtt taacagagtt cccattgctt ataaagctca ttgacgccaa ggacaagctg
     4141 tcggttcagg tccacccgga taacgcttat gcgagagagt ttgaaaactc aaacggcaag
     4201 accgaggcat ggtttgtctt agacgccgaa gaggacgcat gtatagttta cggttttaac
     4261 agagacataa caaaggaaga gtttaaggat agaatcaaaa acggcagcct ttccgaagtg
     4321 ctgaacaccg tcaaagtcaa aaggggagat gtaatctatc tgccctcagg taccgtgcat
     4381 gccataggct ctggtattct gcttgcagag gttcagcaaa gctccaacct tacatacaga
     4441 gtatatgatt atgaccgcat agataaaaac ggctgcaaaa gagagcttca tatagacaag
     4501 gccgtcgagg tcgcaaacac cgcaaaggca gaggcgaact ttgagcctga aggagaaaga
     4561 agtgaaatct gcggcggctg caaaaccctg cttaaaaaga gcgaatactt cacaatggag
     4621 cttatagagg tagaaagcag aaccgaagag tacgcgaccg aggagagctt tacatccttc
     4681 cttgtacttg aaggcagcgg cgccgtcggc tgcggcggcg aggttatgcc cgtaaaaaga
     4741 ggcgacagca tcttcattcc cgcatgcaaa gataaaatag ccttggcggg cagacttaag
     4801 ctcttaaaga ccaccctgta atattttgtc acactttgat taatcctgcc ttaaatgttt
     4861 acaaataatt aacctgattc tattgctatt ttgagcagaa tgatttattt tataggtata
     4921 aaactttaaa actacaggag tgttgtagga tgaaaaaaag cacaaagcgc attttgagct
     4981 tacttctggt cgcagcaatg tccgttgtaa tggttttctc ctcatttgct gccgacgcaa
     5041 ttgatgtaac tcagcccact gacatccagc ttcagtttaa cgaagacgga aagttcagaa
     5101 tcattcagtt tgcagactgc caggatatag cactgcattc accgcttatg gtaaacttca
     5161 ttgagaagac tcttgaagtt tacaaacccg accttgtagt atttacaggc gacaatgtgg
     5221 ttcagcccct tgctcctctg aacaagatgg caattgaggc tattatcgac ccggttgcaa
     5281 aggcaggcat tcccttcacc tttacattcg gcaaccacga cgctgagtca atctccaagg
     5341 agagaatgta tcaggtttac agaagctatc ccaactgcat agcatacgac gcagaccctg
     5401 atctttcagg catgggaacc tgcaaccata caattctcag ctccgacggt tcaaggattg
     5461 ctttcaacat ctggattttt gactccaata tgtatgagta ctccaccggc aagagagagt
     5521 acgactttgt tcacgaggac cagatagagt ggtacaagag aacaagcgag gctcttgagg
     5581 ctcaggcagg ctacaaggtt ccttcccttg cgttccagca cattcccgtt ccggaaatct
     5641 atgagctgta tctcgaggct cagcccggtg acagcccgac aaaggaatac aacggcaaga
     5701 cctatgccct caagctcaac cccgaaatgg caacaggcta tctgggtgag ttctcctgcc
     5761 cgcccgttgt aaacagcggt cagttcgacg cattcgttga gcgcggtgac cttctgggca
     5821 ttgctttcgg ccacgaccat ataaacagct ttgtaggcac atataagggc attgacctta
     5881 ttcagaccgc aggcgtttca atgcactcct acggcgatga cgccgtcaga ggcgcaagac
     5941 tcttcgttct tgacgagaac ggcacatacg agacagaggc ctttacatac aacgatttgt
     6001 ttacagaagc tgagtttatc gctctcaaga tggctcaggt tcccgcatac ctcgttcagt
     6061 tcgcaaaggt attcctctat ctcctccagt acatcttctc aatttatccc ggaattaagc
     6121 tgtaattaaa cttaccggca ccgctcaaac ggtgccggtt tttttatgcc ttaaagcgcg
     6181 aggttagata tttctttgat tgatttgcct aatcaataca cgcatactct gtaaacctaa
     6241 cataa
//
LOCUS       DAONVQ010000115         6104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9654_length_6104_cov_4.991569, whole genome shotgun sequence.
ACCESSION   DAONVQ010000115 DAONVQ010000000
VERSION     DAONVQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9654_length_6104_cov_4.991569"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLD98_07390"
     CDS             <1..379
                     /locus_tag="PLD98_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06101.1"
                     /translation="AEKNKWGYDALFLTVKILSMMKKEERSLSELIKYLPKFMIIKKA
                     IPLNFSPSKLTEVFAKSGGAIPITADGKTIKIEAGKGRAVLAPTRTGKMLKVYSEGHD
                     TEIAGELAFEIEKIINSANIDIK"
     gene            673..2436
                     /locus_tag="PLD98_07395"
     CDS             673..2436
                     /locus_tag="PLD98_07395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HQA06102.1"
                     /translation="MQTTKIKKTTTSKKQSDMPLYSKRSPARKKATSEKGKPVKIAFL
                     GGLNEIGKNMTLIEYDDDIIIIDCGLAFPDSDMLGVDLVIPDFTYVERNADKLKGVLI
                     THGHEDHIGGLAFLLKTVKVPVYATKLSIGLIEGKLKEHKILEDAKLNVIKPGQKFKL
                     GKFEIEAIHVNHSIPDSVSYAIKCDAGTILHTGDFKIDTTPIDGEVIDLSRFAELGKE
                     GVLCMLSDSTNAEREGYSASESKVGDSFGNLFSKAGKRRLIIATFASNIHRIQQIIDV
                     AHKTGRKVALSGRSIEYVVKISTELGYLNIPEGLLIGIEKINNYNDDQLVIITTGSQG
                     EPMSALTRMAMSEHRKVDIGANDYVIISATPIPGNEKTVSKVINELIKLGAEVIYEKM
                     HEVHVSGHAYQEELKLMMALVKPKYFIPIHGEQKHLRKHAALAELIGIPSENIVIADI
                     GSQVEFLGDSMRVSTGIPAGRVFVDGYGVGDVGSIVLRDRKLLSQDGIIVISFVIDSV
                     SREIISPPEITSRGFVYVRESEQLFSAASEQALHIAQRVLDNDSYDLNAVRTRVRDDI
                     AKFMYEKTKRSPMVLPVILEV"
     gene            2553..2993
                     /gene="rplI"
                     /locus_tag="PLD98_07400"
     CDS             2553..2993
                     /gene="rplI"
                     /locus_tag="PLD98_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQA06103.1"
                     /translation="MQVILTQDIKNLGKKGEMVNVSDGYARNYLFPRKLATEVNAQAL
                     TELKNREAAKQYKIDTEIANARANAEKLEGKTVKITAKAGQSGKLFGSVTSKEIAEAI
                     KEQIGIEIDRRKISVAEIKAYGTYPCEIKLYTGISATMYVAVGE"
     gene            3034..4398
                     /gene="dnaB"
                     /locus_tag="PLD98_07405"
     CDS             3034..4398
                     /gene="dnaB"
                     /locus_tag="PLD98_07405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQA06104.1"
                     /translation="MIDSVSIDNNALPYSLEAEQAVLGALLLDPPSLSKVAMFLKPEH
                     FYLEHHKKIYSTIINLDAAKGGKFDVLLVLEALKAENIYSDEAGKAYLLQLSQSVPSA
                     ANVEAYAKIVKEKYYIRTLVKVSEEIIKTATSGEESADVLLDNAEQRIYDIRQGRGVV
                     GPSRFSEVIGLVYEKLYQLSSDERHLYAGLSTGFSDLDRVITGLNKSDLILIGARPAM
                     GKTSFALNLARNVAVKGNKKVVFFSLEMSKEQLAQRVLAMEARIRSHKMRTGNFTPKE
                     WEQLASAAAFLSNCELYFDDTSNITVPEMKARIRRLKDVDCVFIDYLQLMQSSKRTEN
                     RVQEVSEITRSLKLMAKDLNIPVVTCAQLARATEARGRSKKPQLSDLRESGSIEQDAD
                     IVIMLYREDYYQKMEGETEVSDINKAELLIQKNRHGPTDTVELAWNPEYTLFTSIERE
                     REDE"
     gene            4440..5810
                     /gene="tilS"
                     /locus_tag="PLD98_07410"
     CDS             4440..5810
                     /gene="tilS"
                     /locus_tag="PLD98_07410"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQA06105.1"
                     /translation="MTEKVLRFIKDNMLLKSGDSVIVAVSGGADSIALLHLLKQNEKR
                     LGIEVSAAHVNHGIRGDEAKRDENFVIEFCEKRSIPLKVFHEDIPSLAKKLKIGEEEC
                     GRKRRYELLCGIDDFSLIATAHNLNDNLETVLLNLTRGTALTGLCGIPVKRGRIIRPL
                     LNCTREEIEAYCKENSLEYVTDSTNDDAKYSRNKIRKFVIPKLQDINPSILENFAEFL
                     ENIKRDEVYLNEQAQRLLSEAVENGKFKSEAFKKAHPAVRYRAIKAVISNYISGGIEK
                     RHIEKADELLSKCGSFDLCETHRVTSDGKYLFVEEKASLKSAKPPEPIAINKTDITEK
                     ETEFKFADFIITLRLISKTEIDSLQKINNEVCYNLLDYDKMTSAIVIRGRKSGDRLRI
                     KERGLSKTLKKLYSENKLPQSLREINPIIADDEGVVFVNGFGADERCAADNNSKKIIL
                     VSVRRK"
     gene            5830..>6104
                     /locus_tag="PLD98_07415"
     CDS             5830..>6104
                     /locus_tag="PLD98_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003379181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQA06106.1"
                     /translation="MLKDIDRILLSKEELDEAVKRLGRQISEDYRERNLLLVSVLKGS
                     VVFMADLMRAIDIPCRIDFMCVSSYGSGTKTSGTVRILKDLDIDIEG"
BASE COUNT         1892 a         1305 c         1424 g         1483 t
ORIGIN      
        1 cgccgagaag aacaaatggg gatatgacgc tctgttcctg acagtaaaaa tcctttcaat
       61 gatgaaaaaa gaagaaaggt ctttgtcgga gcttataaaa tacctgccta agtttatgat
      121 aattaagaag gcgattccgc ttaacttctc gccttctaag cttaccgagg ttttcgcgaa
      181 gtccggagga gcaattccta ttaccgcgga cggaaaaact ataaaaattg aggccggcaa
      241 gggcagagcg gtgcttgctc ccacaagaac gggaaagatg cttaaagtct actccgaggg
      301 gcacgacacc gagatagcgg gcgaacttgc ctttgagata gaaaaaataa taaacagtgc
      361 caatattgac atcaaatagc ataaatagta caatttatac ggtatgctgg gcaaagcata
      421 cctacataat atgctgtaaa taaaatagaa ttgcagctta aaatatattc gatttaattt
      481 ttatttgaac attgagtctt gagcagagtc aagacttttt aataaacaac agccaaacac
      541 atttgaatga aaatcccttt cttgaggggt tagttgtcta ttgctaaaat tttgagatcc
      601 cggcatagtt taatgcgtgg gggagctgag tattcccgtc aaatgtgttt tgggctaaga
      661 taaggagata atatgcaaac aaccaaaatc aaaaagacaa ccacatccaa aaaacaaagc
      721 gatatgccgc tttattcaaa acgcagtccc gcgagaaaga aagcgacatc cgaaaagggc
      781 aagcccgtta aaatcgcttt tctcggcgga cttaacgaaa taggaaaaaa catgactctc
      841 atagagtacg atgatgatat tatcatcata gactgcggtc tcgcgttccc agacagcgat
      901 atgctcggcg ttgaccttgt cattcccgat ttcacatatg tcgagcgcaa cgccgacaag
      961 ctcaaaggtg ttctcattac acacggacat gaggaccata tcggaggact tgcgttcctt
     1021 ctcaaaaccg taaaggttcc cgtttacgcg acaaagctgt ctattggtct tatcgaaggt
     1081 aaactcaaag agcataagat tcttgaagac gcgaagctca atgtaataaa gccaggtcag
     1141 aagtttaaac tgggcaagtt tgaaattgag gcgattcatg taaaccattc gattcccgac
     1201 tcggtttcat acgccatcaa gtgcgacgcg ggcacaattc tgcatacagg cgactttaaa
     1261 atagacacta cgccgattga cggcgaggtt attgaccttt cccgctttgc cgagcttggc
     1321 aaggagggtg tgctttgtat gctttccgac tccacaaacg cggagcgtga aggctattcc
     1381 gcaagcgaaa gcaaggtcgg cgactctttc ggaaatcttt tctctaaggc tggaaagaga
     1441 aggcttatta tcgccacctt cgcgtccaat attcacagga ttcagcagat aatcgatgtc
     1501 gcgcacaaaa ctggcagaaa ggttgccctc tctggacgca gcattgagta tgtcgttaaa
     1561 atcagcacag agttgggtta tctcaatatc cccgaaggtt tgcttatcgg cattgagaaa
     1621 atcaacaact acaacgacga ccagcttgta ataatcacca cgggcagcca gggcgagcct
     1681 atgtccgctc tgacaagaat ggctatgtcc gagcacagaa aagttgatat aggcgcgaac
     1741 gactatgtca ttatttccgc aacgcccatt cccggcaacg aaaagactgt aagcaaggtt
     1801 ataaacgagc taattaagct cggcgctgag gttatttacg aaaaaatgca cgaggtgcat
     1861 gtttctggcc acgcttatca ggaagagctt aaactcatga tggctcttgt aaagcccaaa
     1921 tactttatac ctatccacgg tgagcaaaag catctcagaa aacatgcggc tcttgcggag
     1981 cttatcggaa tccccagcga aaacattgtc attgccgaca tcggctctca ggttgagttc
     2041 ctgggcgact ctatgcgagt atccacaggt attcccgctg gcagagtatt cgtcgacggc
     2101 tacggcgtcg gcgatgtggg cagcattgtg cttcgcgaca gaaagcttct ttctcaggac
     2161 ggcataatag ttatttcttt cgttatagat tcggtttcaa gagaaataat ttctcccccc
     2221 gaaatcacat caagaggctt tgtatatgta agagagtccg agcagctgtt cagtgccgcc
     2281 agcgagcagg ctctgcacat cgcgcagaga gtgcttgaca acgattccta tgaccttaat
     2341 gccgttcgca caagggtcag ggacgacatc gctaagttca tgtacgaaaa aaccaagcgc
     2401 agcccgatgg tcctgccagt tattcttgag gtttaattca cagctttttt aaagcagatg
     2461 atgactaata gggtttttta tgatacacta agttaataaa tactaaaatt accctatgta
     2521 gctttttaaa gttacagaaa ggatttgata ttatgcaggt tattttgact caggacatta
     2581 aaaacctcgg caaaaagggc gagatggtaa atgtttcgga tggttatgcc cgcaactatt
     2641 tattccccag aaagctcgca accgaggtta acgctcaggc tctgactgag cttaaaaaca
     2701 gagaggcggc aaagcagtac aagatagaca cagagattgc caacgccaga gcaaatgccg
     2761 agaagctgga aggcaagact gtaaagatca ccgcaaaagc gggtcagagc ggcaagctct
     2821 tcggctcggt aacttcaaag gaaatcgccg aggcaataaa agagcaaatc ggcattgaaa
     2881 ttgaccgcag gaaaatcagc gtagccgaaa tcaaggctta cggtacatac ccctgcgaga
     2941 taaagcttta tacaggcatt tccgccacta tgtatgttgc tgtgggagaa tagcatctta
     3001 acctttattt caacggggtg ctaagttaaa acaatgattg attccgtttc tatcgacaat
     3061 aacgcattac catacagctt ggaggctgag caggcagtgc tcggcgctct tttgctcgac
     3121 ccgccttctc taagcaaggt ggcaatgttt ttaaagcccg agcattttta tctggagcac
     3181 cataaaaaga tttattccac aataataaat cttgacgctg ccaagggcgg caagtttgat
     3241 gtattgcttg ttctcgaggc tttaaaggct gagaatatct actcagacga ggctggaaag
     3301 gcgtatctcc tgcagctatc ccagtcggtt ccctccgcag cgaatgttga agcctacgcg
     3361 aagatagtca aagaaaaata ctacataaga acgcttgtaa aggtttccga ggagattatt
     3421 aaaaccgcaa ccagcggcga agaaagcgcc gatgttctcc ttgacaatgc tgagcagaga
     3481 atttacgaca taaggcaggg caggggcgta gtcggcccct caaggttcag cgaagtcata
     3541 ggtcttgttt atgaaaagct ctatcagctc tcctccgacg aaaggcacct ttatgcgggg
     3601 ctttccacag gtttcagcga ccttgacagg gttattacgg gacttaataa gtcggacctt
     3661 atacttatag gcgcccgccc cgcaatgggt aagacaagct ttgcgctgaa tcttgcgaga
     3721 aatgttgctg taaagggcaa taaaaaggtt gttttcttca gtcttgaaat gtccaaggag
     3781 cagcttgccc aaagggtcct tgccatggag gctagaatca ggagccacaa aatgcgcacg
     3841 ggcaacttca ctcccaaaga gtgggagcag cttgcctccg cggcggcttt cctaagtaat
     3901 tgtgaattgt actttgacga tacatccaac ataaccgtgc ccgaaatgaa ggcaagaatc
     3961 aggcgtctta aagatgttga ctgcgtattc atcgactacc tacagcttat gcagtccagc
     4021 aagagaacag agaacagagt gcaggaggtt tccgaaatta caaggagcct caagctcatg
     4081 gctaaggatt tgaatatccc tgtggtaacc tgcgctcagc tggcaagagc tacggaggca
     4141 aggggcagat ccaaaaagcc tcagctctcc gacctaagag aatctggctc aattgagcag
     4201 gacgccgata tagtcatcat gctttatcgt gaggactatt atcagaagat ggagggcgaa
     4261 acggaagtat cggatataaa caaggcagag cttttgattc aaaagaacag gcacggcccc
     4321 accgataccg tagagcttgc gtggaatccc gaatatacac tgtttacttc tatagaaaga
     4381 gaaagagagg acgaatagcc tctctttata tttaatacca cagtttgcaa agggtaaaaa
     4441 tgacggaaaa ggttcttaga ttcataaaag ataatatgct cctcaagagc ggcgacagcg
     4501 taattgtcgc cgtatcggga ggggctgatt ctattgccct tttgcatcta ttgaagcaga
     4561 acgagaaaag gctcggcatc gaggtttccg ccgcccatgt taaccacggc atcagaggcg
     4621 acgaggcaaa aagagacgag aactttgtaa tagagttttg cgaaaagcgc tcgattcctc
     4681 tgaaggtttt ccatgaagac attccctccc tcgctaaaaa gcttaagata ggggaggagg
     4741 aatgcggcag gaaaagaaga tatgagcttt tatgcggcat tgacgatttt tcgcttattg
     4801 ccaccgccca caatcttaat gacaaccttg aaacggtgct tttgaattta acaagaggca
     4861 cagccttgac ggggctttgc ggaatccccg ttaagagagg cagaataatc cgccctttgc
     4921 ttaactgcac aagggaagaa attgaagcct actgcaaaga aaactccctt gaatatgtaa
     4981 cagacagcac aaatgatgac gcaaaatatt caaggaataa aattagaaaa tttgttattc
     5041 ctaaacttca ggatattaac ccatctattt tagaaaactt cgctgagttt ctggaaaaca
     5101 ttaaacggga tgaggtgtat ttaaacgaac aggcacagcg cctcttgtcc gaagctgttg
     5161 aaaatggtaa atttaagtcg gaggctttca aaaaagccca ccccgcggta agatacaggg
     5221 caattaaagc tgtcatttcc aactatatct ccggcggaat cgaaaaacgc catatagaaa
     5281 aagcggatga acttctaagt aaatgcggca gctttgacct ttgcgaaacc cacagggtaa
     5341 cctctgacgg caaatatctt tttgttgagg aaaaggcaag tcttaagtcc gcaaagccgc
     5401 cggagccgat agcaattaac aagactgata ttaccgaaaa agaaaccgaa tttaaatttg
     5461 cggattttat aatcacgctc agacttatta gcaaaacaga aatagattcg ttgcaaaaaa
     5521 ttaataatga ggtctgttat aatctgttag attatgataa aatgacatct gctatcgtca
     5581 ttcgcggcag aaaatcggga gacaggctca gaatcaaaga aagaggttta tccaaaaccc
     5641 tcaaaaagct ctattccgaa aataaactgc cacaatcttt gcgggaaatt aatcctatta
     5701 ttgcggatga tgagggcgta gtctttgtaa acggcttcgg agccgacgaa aggtgcgctg
     5761 cggataataa cagcaaaaaa atcatacttg tttctgtaag gagaaaataa tatgcaggtt
     5821 gaaaatatca tgcttaaaga tattgacaga atacttctca gcaaggaaga gcttgacgaa
     5881 gccgttaaaa ggctcggaag gcagatttca gaggattata gagagagaaa cctgctgctt
     5941 gtcagcgttc ttaaaggctc tgtagttttc atggcagacc ttatgcgtgc tatagatata
     6001 ccctgccgca tagactttat gtgtgtttcc agctacggca gcggcaccaa gacctcgggc
     6061 actgttagaa ttctcaagga tttagacatt gacatagagg gtta
//
LOCUS       DAONVQ010000116         6099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9660_length_6099_cov_5.171906, whole genome shotgun sequence.
ACCESSION   DAONVQ010000116 DAONVQ010000000
VERSION     DAONVQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9660_length_6099_cov_5.171906"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /gene="mltG"
                     /locus_tag="PLD98_07420"
     CDS             complement(<1..1470)
                     /gene="mltG"
                     /locus_tag="PLD98_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQA06107.1"
                     /translation="MNDFNSGSNKGKYNDYSRFDELFPDFAIRDDSDDTKELRGSEIK
                     KSADINSKSASQEKGTKPNRKGVYFSTGPVRTGSTQSRPQRPAPQPMPISKAPARQQG
                     KAPAKQKGKVPPIQQKTQTAVNKVGTLAKNIGKNIPKDKLPDKQKISELAARFLTIKV
                     VTIILVIILSSATIVNITLKCINDILAISRSDEIITVEINDNMTTKDIISVLHDKGLI
                     KYKSFCLLVSDFLGYKDTKYIPGAYELSPNMGLEGMLNILKSARTTNKTVRLTFPEGY
                     TVDQIIDKLASYGVASKANLLKAFQELSYYNNFDFIQGIKNRDVRYRLLEGYVYPDTY
                     EFYIGENATSVVRRFLTNFQNKWTDEYDARLKELNMTLDDLIILASIIEKEAYGTEQM
                     YLISSVLHNRLNDKSNLYSYLGCNATGDYISSIPKDSISNADFIRLHKLYDTYQHKGL
                     PVGPICNPGTEAIRAALYPPETPYMYFRHDNKRNIYMART"
     gene            complement(1500..2087)
                     /locus_tag="PLD98_07425"
     CDS             complement(1500..2087)
                     /locus_tag="PLD98_07425"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HQA06108.1"
                     /translation="MPAELLDIRVEKNKLREQYKNIRANIENKEAKDLKIKNKLSNLW
                     AYREAERVFAYVSMGTEVSTFEIIKMALQKGKKVAVPYCVPGTRELRFYYINSLDDLE
                     SGTFGVLEPKTDKCEIATDHSGICIVPGLAFDRSGHRLGYGKGYYDRFLNSFNGCTVG
                     LTYQDCIAEKLPSGRYDVKIDIIIYEGGFIRTNGR"
     gene            complement(2069..2620)
                     /locus_tag="PLD98_07430"
     CDS             complement(2069..2620)
                     /locus_tag="PLD98_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HQA06109.1"
                     /translation="MIYVFLADGFEEIEALVPVDLLRRAEIAVSTVGIGSKKIRGSHG
                     IEVICDFEAEEVSPNNDLVGIILPGGMPGTKTLGNTATVHDFIDYCAQNHLLIAAICA
                     APSILGKKGLLEGKRAVCFPSFEKDLLNAIIAYGYVCTDDNFITAKGAGSSIEFALEI
                     VRYFKGDREARRLRDSIQCPPSY"
     gene            complement(2708..3670)
                     /locus_tag="PLD98_07435"
     CDS             complement(2708..3670)
                     /locus_tag="PLD98_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06110.1"
                     /translation="MIAVFSEGSGIKHYYFGELMDYTFYRNIKTDTESLLKLLTESFV
                     CKSETAQLFKEKVLKHSNIFVSLKDNEPSFALIGVPVEINTGSEELKGVYIIPAVKNE
                     GLKIEHKDEIAAWLSEISNHFSAECLVFSPNDEAVSVIFSELGFKNSFTEKILKISKY
                     SAKVLASKDEVTTDKLSFTHFDELSHKFKGAFIKWNNDLFEYFVKSLETAGGEALSLS
                     SGGDVKGYIFGKKSGCIGSIFESVGDGSVLSFALSRIARDEEILECEIFLPLSYPFSA
                     DNMDVVSGAMIKPLSEKAENALSKAPLYFGYSPFRRILYSSRMK"
     gene            complement(3798..4148)
                     /locus_tag="PLD98_07440"
     CDS             complement(3798..4148)
                     /locus_tag="PLD98_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQA06111.1"
                     /translation="MVIRNSEMSVNVNTNMRGGEGNVKITKIVEKPHYDGNSRLIATI
                     HLEPGASIGTHVHEGEEEIFYVLSGEALYNDNGTDVVMHEGDSCVCRNGESHGLKNNR
                     TGRELVVMAIILTL"
     gene            4317..5531
                     /locus_tag="PLD98_07445"
     CDS             4317..5531
                     /locus_tag="PLD98_07445"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HQA06112.1"
                     /translation="MNILVINAGSSSLKYQLINMDGEKVIAKGLCERIGCEGGRLEAS
                     NGNGKKYVKEVDMPDHTVAFLEVKNALLSGECKVIDDLSEITAIGHRVVQGGSLFNKS
                     VLIDDEVIKGIESLIDLAPLHNAAHLQGINACTAVFGKDVPQVAVFDNAFHSTMPPEA
                     YIFPIPYEYYEKYKVRRYGFHGTSHRFVSARCAELMGKDITELKIISCHLGNGASITA
                     IKNGKVIDTSMGLTPLDGFMMGTRTGSLDPSVVTYLQEKEGWTPEETSSILNKKSGVL
                     GISGVSNDDRDVRAAAAEGNERAKLARAMQWYQIRKYIGSYIAAMEGVDAIAFAGGLG
                     ENTTDLRENICEKFAFMGVEFDREANLTAVKGKEAEITKPGSKIRVFVIPTNEELLIA
                     RDTKDIVMALNK"
     gene            5726..>6099
                     /locus_tag="PLD98_07450"
     CDS             5726..>6099
                     /locus_tag="PLD98_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06113.1"
                     /translation="MYSINPKEFNSVFAVPAAVVDRHIKLAGSAQLKALLWIYRHSCE
                     PIDIEKMSKAIGISAADVKDALQYWLECGLLIEDGKENAAAENAKPEEKTEIKEITPA
                     PAKKEPAQIHESEKNLPDLPVL"
BASE COUNT         1609 a         1424 c         1380 g         1686 t
ORIGIN      
        1 ggtccttgcc atatagatgt ttctcttgtt gtcgtgcctg aagtacatat acggcgtttc
       61 gggcggatac agagccgctc ttattgcctc agtgccgggg ttgcagatag gtcctaccgg
      121 cagacccttg tgctgatagg tgtcatacag cttgtgaagc cttatgaaat cggcattcga
      181 aatgctgtct ttcggaatag acgagatata atcgcccgtc gcgttgcagc ccagatagct
      241 gtaaaggttc gatttgtcgt tgagcctgtt atgcagaacg gaggaaataa ggtacatctg
      301 ctccgtaccg taggcctctt tttcgatgat ggacgcgaga attataaggt cgtcaagggt
      361 catgttaagc tccttgagcc ttgcgtcata ctcatcggtc cacttgttct ggaagttcgt
      421 aaggaatcgc cttacaacgc tggtggcgtt ttcgccgata tagaactcgt aagtgtcggg
      481 atatacatag ccttcaagca ggcggtagcg gacatccctg tttttgattc cctgaataaa
      541 gtcaaaattg ttatagtagc ttagctcctg gaaagccttt aggagatttg cctttgaggc
      601 gactccgtag gacgccagct tgtcgataat ttggtccacc gtgtagcctt cggggaatgt
      661 aagccttacc gtcttgtttg tggttcttgc tgatttaagt atattcagca ttccctcaag
      721 acccatgttt ggcgacagct cataggctcc ggggatatac tttgtatcct tgtagcccaa
      781 aaagtcagaa accagtaagc agaaagattt gtatttaata agacccttgt catgcagcac
      841 cgaaattata tccttcgtgg tcatgttgtc gttaatttcc acggtaataa tctcgtcgct
      901 gcggcttatg gcaagtatgt cgtttatgca ctttaatgtg atgtttacga ttgtcgccga
      961 tgaaaggata ataacaagaa taatggtgac aaccttgatt gtaaggaacc ttgcggcaag
     1021 ctcgcttatc ttttgcttgt cgggcagttt atctttggga atgtttttgc cgatgttttt
     1081 agcaagcgtg ccgactttgt ttaccgcggt ctgagttttc tgctgtatgg gcggtacttt
     1141 gcccttttgc tttgcgggag cttttccctg ctgtcttgcg ggagccttgc ttataggcat
     1201 cggctgcggt gcaggtcttt gcggacggct ctgagtactg cctgtcctta cgggacctgt
     1261 ggaaaaatat acgccttttc tattcggctt tgttcctttt tcctgtgaag cggatttact
     1321 gtttatatca gcggactttt ttatttcact gccgcgtaat tctttggtat cgtcactgtc
     1381 atcacggata gcaaaatcgg ggaaaagctc gtcaaagcgg gagtagtcat tgtattttcc
     1441 cttattgctt cccgaattaa agtcgttcat aggcaaatcc cttcgtaatc ctcaaaattt
     1501 caacggccgt tcgttcttat aaagccgcct tcgtaaatta ttatgtcaat tttaacatca
     1561 tacctgcccg aaggcagttt ttcggcgatg caatcctgat aggtcagccc tacggtacag
     1621 ccgttaaaac tgtttaaaaa tctgtcgtaa taacctttgc cgtagcccaa gcgatgacca
     1681 gagcggtcaa aggcaagtcc gggaacaatg caaatgccgc tgtggtctgt ggcaatctcg
     1741 cacttgtccg ttttgggctc aagcacccca aaggtgccgg actcaaggtc atccaaagaa
     1801 tttatataat aaaacctaag ttctctcgtg ccggggacgc agtagggcac tgcaaccttt
     1861 ttaccttttt gcaaggccat cttaattatc tcaaatgtag aaacctcggt ccccatcgaa
     1921 acataggcaa agaccctttc cgcctctctg tatgcccaca gattggacag cttattcttt
     1981 atcttcaggt ccttagcttc tttattctca atgtttgccc ttatgttttt atactgctcc
     2041 ctgagtttat tcttctcaac tcttatgtct aatagctcgg cgggcattgt atgctgtccc
     2101 gaagccttct ggcctcgcgg tctcctttaa agtatctcac gatttcaagg gcaaattcaa
     2161 tcgaggagcc tgctcccttc gccgtaatga aattgtcgtc ggtgcacaca tagccgtaag
     2221 ctatgattgc gtttaacagg tccttttcaa acgacgggaa acacacagcc cttttgccct
     2281 caagcagacc ctttttgccg agtatggagg gagctgcgca tatggcggct ataagcaggt
     2341 gattctgcgc gcagtagtcg ataaaatcgt gaacggttgc ggtattgccg agagtttttg
     2401 tgccgggcat accgccgggc agaattatgc ccacaaggtc attgttgggg cttacctctt
     2461 ccgcctcaaa gtcgcagata acctcaatgc cgtgggagcc tctgattttc ttggagccaa
     2521 tgcccacagt agagacggcg atctcggcgc ggcgaagaag atctacaggc acaagggctt
     2581 caatctcttc aaatccgtct gctaaaaaaa cataaatcac ggcaaatttc ctttctatct
     2641 ttctataatg agagtttcat tacagctaag atttatgtaa aagtagtcag cttataatgc
     2701 ttattaatta cttcatcctg ctgctgtaga gtatgcgcct gaaaggcgaa tacccgaaat
     2761 aaagcggagc tttggaaagc gcgttttcag ccttttcgct taaaggtttt atcatcgcgc
     2821 ctgaaaccac atccatattg tccgcggaaa aaggatagct cagcggcagg aaaatctcgc
     2881 actcgagaat ttcctcgtcc cgagctatgc gagaaagagc aaaagacaaa accgagccgt
     2941 cgcccaccga ctcaaaaatc gagccgatac agccgctctt tttaccgaat atatatcctt
     3001 taacatctcc gccgctgctg agacttaaag cctcaccgcc ggcggtttcc aaggatttta
     3061 cgaaatactc gaaaaggtca ttattccatt ttataaaagc gcctttaaac ttatgcgaaa
     3121 gctcatcaaa atgcgtaaag ctcagtttgt cagtggttac ctcatcttta gaggcaagta
     3181 ccttagcgga gtatttgctg attttgagta tcttctccgt aaaactgttc ttaaatccca
     3241 attcggagaa aataacgctt acagcctcgt cattggggga gaaaacaaga cactctgcag
     3301 agaaatgatt tgaaatctcg ctcagccacg ccgcaatctc gtctttgtgc tcaatcttga
     3361 gcccctcgtt tttaactgcg gggataatgt atacgccttt aagctcctca gaacccgtat
     3421 taatctctac gggcacgccg ataagagcga aactcggctc attgtcttta agacttacaa
     3481 agatgtttga gtgtttcagc accttttctt tgaaaagctg agccgtttcg cttttgcaaa
     3541 caaaagattc ggtaagaagc tttaaaagac tttccgtatc tgtttttatg ttcctgtaaa
     3601 aagtataatc cataagctct ccaaaatagt agtgcttaat gccgctgcct tccgagaata
     3661 cggcaatcaa aggcaaaaca cgcttaatgg gcctttgaaa tcaaaacttg gttcttttct
     3721 ttgcaaagcc catagcgctg caattatatt ataacaatta ttgaccgata cttcaagttt
     3781 tatggcaaaa ccttacatta cagagtaaga attatcgcca taaccacaag ctcgcggccg
     3841 gtgcggttgt tcttaagacc gtgggattcg ccgtttctgc aaacgcagct gtcgccctca
     3901 tgcattacaa catcagtgcc gttgtcgttg taaagagcct cgccgctgag gacatagaag
     3961 atttcttcct cgccctcatg cacatgggtg ccgatagatg cgcccggctc taaatgaatg
     4021 gtggcaatga gccttgagtt tccgtcataa tggggctttt cgacaatctt tgtaatctta
     4081 acattgccct cgccgccgcg catattggtg ttaacattta ccgacatctc gctgtttctt
     4141 ataaccatcg ttattacccc ttaaatcagg attgtttttg ggattttgtt acatttaatc
     4201 ataaaacatg ctttgacaaa acaggacaaa actattgcaa tattataatg ttttgtatat
     4261 aatatatcag gtttgtgcaa tctttcaagt atctacaaag taaagggtgt ttttttttga
     4321 atattttggt tatcaacgca ggcagttcat cactaaagta tcagctaatc aacatggacg
     4381 gcgagaaggt tatcgccaag ggtctttgcg aaagaatcgg ctgcgaaggc ggcagacttg
     4441 aagcatcgaa cggtaacggc aaaaaatatg ttaaagaagt agacatgccg gaccacaccg
     4501 ttgcattctt agaggttaag aacgctctct tatccggcga gtgcaaggtc atagacgacc
     4561 tttccgaaat caccgctatc ggtcacagag tagtgcaggg cggttctctc tttaacaaaa
     4621 gcgtacttat agacgatgag gtaatcaaag gcatagagag tcttatagac ttggctccgc
     4681 tgcataacgc agctcacctt cagggcatta acgcctgcac ggcggttttc ggaaaagatg
     4741 ttccccaggt cgccgttttc gataacgcgt tccacagcac catgcctccc gaggcataca
     4801 ttttccccat tccttacgag tattacgaga agtacaaggt aagaagatac ggcttccacg
     4861 gcacctccca ccgctttgta agcgcaagat gcgcagagct tatgggcaag gatattacgg
     4921 agcttaaaat aatctcctgc caccttggca acggcgcttc aatcaccgcc ataaagaacg
     4981 gcaaggttat cgacacaagc atgggtctta ctccccttga cggctttatg atgggcacaa
     5041 gaacaggctc ccttgacccg tcggtagtta cctaccttca ggagaaggaa ggctggacac
     5101 ccgaggagac cagcagcatc ctcaacaaaa agtcgggcgt tctgggcatt tcgggcgtat
     5161 ccaacgacga cagagatgta agagcagccg cagccgaagg caatgagcgc gcaaagctgg
     5221 caagagcaat gcagtggtac cagataagaa agtatatagg cagctacatc gccgctatgg
     5281 agggcgtgga cgccatagca ttcgctggcg gtctgggcga gaatacgaca gacctgcgcg
     5341 aaaacatctg cgaaaagttc gcattcatgg gcgttgagtt tgacagagag gctaacctca
     5401 ccgccgtaaa gggcaaagag gcagaaatca caaagcccgg ctcaaaaatc agagttttcg
     5461 taatccccac caacgaagag ctgcttatcg caagagatac gaaagatatc gtcatggctc
     5521 ttaataaata gactaagttc aataaataca aaagctgaga gcattagccc tcagctttta
     5581 tttgcccctt taaactcctt tgttcttaaa tattaggctt gtacatataa atttatcgcc
     5641 caagaaatat ttggctttta ttttatgtgg acttttgtta ttatagtaaa gaatcagaac
     5701 cttagctgaa tgccggaggt cggaaatgta ctcaatcaat ccaaaggaat ttaacagcgt
     5761 atttgctgtg cctgccgcgg ttgtggacag gcatattaag cttgccggtt cggctcagct
     5821 taaggctctg ttatggattt acaggcattc ctgcgagccg attgatatag agaaaatgtc
     5881 aaaggcaatc ggcatttccg cggcggatgt aaaagacgcc cttcaatact ggcttgaatg
     5941 cggtctgctt attgaggacg gcaaagaaaa cgcagccgcc gaaaatgcaa agcccgaaga
     6001 aaaaaccgaa ataaaagaga taacccccgc tcccgcaaag aaggagcccg ctcaaattca
     6061 cgagagcgaa aaaaacctcc ccgacctgcc cgttctgaa
//
LOCUS       DAONVQ010000117         6023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9808_length_6023_cov_4.598358, whole genome shotgun sequence.
ACCESSION   DAONVQ010000117 DAONVQ010000000
VERSION     DAONVQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9808_length_6023_cov_4.598358"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PLD98_07455"
     CDS             <1..503
                     /locus_tag="PLD98_07455"
                     /inference="COORDINATES: protein motif:HMM:NF027050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HQA06114.1"
                     /translation="KSRNHLDKILSVNVISEGDPLEIYTDSDGEVIFKKYSPMGELSA
                     FTTQYAEVLYRATNMPVIITDRDHVISCVGLPKKDTLDKRVSPALESIMENRSSYIAT
                     SDSQPFIAIEGLDRNAAIVYPIIGGGDVSGSVVLLFNESGTPPSQTDIKLAQVAASFL
                     GKQTEE"
     gene            complement(546..821)
                     /locus_tag="PLD98_07460"
     CDS             complement(546..821)
                     /locus_tag="PLD98_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06115.1"
                     /translation="MEKFKWEIVETEICTEDSQSFRTYGVKKVDQWGFEFVYPDISLV
                     KEDVQNLIKRVGHMDIAREHLLDIIHDYIEELAGVDIYVPNKVLVSA"
     gene            complement(1133..2497)
                     /locus_tag="PLD98_07465"
     CDS             complement(1133..2497)
                     /locus_tag="PLD98_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQA06116.1"
                     /translation="MQKNVFKKSMALMLCLTLLVTAFSFVGTASSADVDFKITNPYET
                     VDWESWDNFRLSLHNHSWVSDGRDNFKDMVERHYELGYDALAMTDHGTVDYGWIGEVN
                     RVDLLETVLELKRKSSEKPYGLTPERYEEITTGVGRNGRPMIRIPYGIEQNPTSLNNA
                     HVNSWFVDYGNAILGGTSDYETPIKEVNKLGGVSVINHPGEYTRAKKENNPDKAYDSS
                     YAYKIDKFANLLIKYHTCIGIDVNSKNDGRTKNDRKLWDILLQKVVPTGRNVFAIGSS
                     DAHSLSAIDTGWVIAVMPENTVENIKTALLTGTFFAGSRHIKNSKELAILSAEVGRDL
                     GKEWEANPEAPEPVVTGIFVDEAEDTIRIEAVNAETIHWIADGKVIAVGNEIDLDDYA
                     DQVGCYVRAEIFGDGGILYSQPFILEHDNAPKEGGVKGFFDFGNLLAMIRKFILTLFA
                     PIIK"
     gene            complement(2718..3071)
                     /gene="spoVAE"
                     /locus_tag="PLD98_07470"
     CDS             complement(2718..3071)
                     /gene="spoVAE"
                     /locus_tag="PLD98_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HQA06117.1"
                     /translation="MMFLKAFIVGGLLCVIGQLIIDKTKLTPGRVLVGYVITGVILGA
                     IGLYEPLKEWAGAGATVPLTGFGNLLAEGTRKAVNEKGLIGALSGPLTAGSVGIMAAV
                     ICGLVVSFFAKPKSK"
     gene            complement(3173..4699)
                     /locus_tag="PLD98_07475"
     CDS             complement(3173..4699)
                     /locus_tag="PLD98_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQA06118.1"
                     /translation="MFSHINSMGLMGLDAFMVDVEADISSGPLRFDIVGLPDAAVSES
                     KERVRAAVNNCGYEFPVSHVTVNLAPADVKKAGPLYDLPIFMALLKATEQISGDLSDT
                     AFIGELSLGGSLRHISGALPMVLAAKAAGIKEVFLPKENEGEVAAITGISIYPAESVE
                     EIVDHINGITALKPLEPVEFRQAQDLSALPDFSQVKGQYKAKRALEVAAAGSHNILMI
                     GPPGSGKSMLAKRLPSILPDMSFEEALESTKVYSIAGLLKGKDTLIRTRPFRSPHHTV
                     SAAGMSGGGSAPKPGELSLAHNGVLFLDELPEFSKAAIESLRQPLEDGVITISRASYT
                     LSYPCSVMLVCAMNPCPCGFLGHPKRKCTCPKGAVQKYLSKISGPLLDRVDIHIEVPP
                     VDFSDLSREGNEESSAAIKERVEKARKLQRERFKGTKITSNARMDSAAVRKFCVLSSD
                     AAEFLKSAFDRLDLSARAYERILKVARTIADLDGKETIEKKHIMEGVQYRSLDRKYWY
                     "
     gene            complement(4813..5691)
                     /locus_tag="PLD98_07480"
     CDS             complement(4813..5691)
                     /locus_tag="PLD98_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQA06119.1"
                     /translation="MLVRFIADTGCDLPSIKKEVACIKLLPVYMIIDGAPYYESINIT
                     LDKFTAENGTLDVKPIVPFQVYLDAIIEAQEDGCSDIFIVTPDKNLFGMYGEAVKAAK
                     LFSRRKVSAGINVNVIDSKTFSVGYGVPLASVALDYESGKFKTAHDIEKALLKRMSET
                     LIVCRSSHGTRIFKRNGDGKNHPEKYIVFRGGYERLTNAPVEERAETIAKKLHSLDSQ
                     DFVVFFLFNRKWPLKNEVQGTAYIMSVVLSDINIKATRSIKPGISTTYAGGMDLSMML
                     ITPASNFEQIDEIRKQ"
     gene            complement(5886..>6023)
                     /locus_tag="PLD98_07485"
     CDS             complement(5886..>6023)
                     /locus_tag="PLD98_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HQA06120.1"
                     /translation="RTVRIPMINDARSLNLSNSVAIGAYEVLRQWGFPDLSLYGKFAN
                     V"
BASE COUNT         1555 a         1418 c         1337 g         1713 t
ORIGIN      
        1 aaaaaagcag aaatcatctg gacaagatac tcagtgtcaa tgttatcagt gagggtgacc
       61 ctcttgaaat ctatacagat tccgacggag aagttatctt taagaagtac tccccgatgg
      121 gcgaactttc ggcattcaca acccagtacg cagaagtatt atacagggcc accaatatgc
      181 ctgtcattat tacagaccgc gaccatgtaa tctcctgcgt gggtctgccg aaaaaggata
      241 cccttgacaa gagagtaagc cctgcacttg agtctattat ggaaaatcgt tctagctata
      301 tcgcaacatc agactctcag ccctttattg caatagaagg cttagaccgc aatgctgcaa
      361 tcgtctaccc aattatcgga ggcggcgatg tttccggcag cgttgtgctc ctcttcaatg
      421 aaagcggtac accgccctca cagaccgaca ttaagttggc tcaggttgcg gcatcgttct
      481 taggtaaaca aacagaagaa taactgcatg attcttacgg gctgcagtct ggcgctgcag
      541 cctttttatg cgcttacgag gactttattt gggacataga tgtctacgcc cgcaagctct
      601 tctatgtagt catgaattat gtcaagaaga tgttccctgg ctatatccat gtgacctact
      661 cttttgatga ggttttggac atcttcttta accagagaaa tgtcggggta aacgaactca
      721 aagccccatt ggtcaacttt ttttactccg taagttctga aagattgcga atcttccgta
      781 caaatctcag tttcaacgat ctcccatttg aatttttcca tagtttgccc ctttttttgt
      841 tgcaaatttt atggaatttg ctaaatgttt cctgtttttt gttattataa gtttaaacct
      901 cggaactgtc aacacgcttt gtgaataaaa ttcacaaaat ttctgataaa aactgataaa
      961 tttcacaaaa attcttctca tagttttagt aaacttaaaa attcgtacgc ataagtgtag
     1021 agaaaaccca taaaaccgag gccgatgcgt ttaattttcc aattcagcaa aaaggcaaaa
     1081 gggtacaaaa aagcacagta caacgaatgt actgtgcttt gatttgcttt aattatttaa
     1141 ttatcggcgc aaagagagtg aggatgaact tgcggatcat agcaagaagg ttgccgaaat
     1201 cgaagaagcc ttttacgccg ccctccttgg gagcgttatc atgctcaaga atgaagggct
     1261 gtgaataaag gattccgccg tcgccgaaaa tctcagctct tacatagcag ccaacctgat
     1321 cggcgtaatc gtcaaggtcg atttcgttgc cgactgcgat aaccttgccg tctgctatcc
     1381 agtggattgt ctctgcgttt acagcttcaa tcctgattgt atcctctgcc tcgtcaacaa
     1441 agatgccggt aactacaggt tccggagcct cgggatttgc ctcccactct ttgcccaggt
     1501 ctctgccgac ctcagcgctg aggattgcca gctccttgga gttcttaata tgtctgctgc
     1561 ctgcaaagaa tgtgcctgtg agaagagcgg tcttaatgtt ctcaacagtg ttttcgggca
     1621 ttaccgcgat tacccagccg gtgtcgattg cgctaaggga atgggcatcg cttgagccga
     1681 tggcaaaaac attcctgccg gtgggaacaa ccttctgaag gaggatatcc cacagcttgc
     1741 ggtcgttctt tgttctgccg tcattcttgc tgttaacatc aatgcctatg caggtatgat
     1801 atttgataag aaggtttgcg aatttgtcta tcttgtaagc atagcttgaa tcgtaagcct
     1861 tatcaggatt attctctttc ttagctctgg tgtactcgcc gggatggttt attacggata
     1921 cgccgccaag cttgttgacc tctttgatgg gggtctcata gtcgcttgtt ccgccgagaa
     1981 tggcgttgcc gtagtcaaca aaccagctat tgacatgggc attgttgagg gatgtggggt
     2041 tctgctcaat gccgtaaggt atgcggatca tgggtctgcc gtttctgccg acgcctgtgg
     2101 tgatttcctc gtacctttcg ggtgtaagac catagggctt ttcgctgctt ttgcgcttaa
     2161 gctcaagaac ggtctcaaga agatctactc tgttgacctc gccaatccag ccgtagtcga
     2221 ctgtgccgtg gtctgtcata gcaagagcgt cgtagccgag ctcatagtgt ctttcaacca
     2281 tatccttgaa gttgtctctg ccgtcggata cccaggaatg gttatgtaag ctgagtctga
     2341 agttgtccca ggattcccag tcaaccgttt cataggggtt ggttatcttg aagtcgacat
     2401 cagcgcttga tgccgtaccc acaaatgaaa aagcggtgac gagaagggtg aggcagagca
     2461 taagagccat gctctttttg aaaacattct tctgcatttg tttcaccttt ccataaaagt
     2521 attaaaacgc actgagactg cgctgcaagc tgttaaatat gtataaagcg caatcaccaa
     2581 ttcttcaaat ttagtatata attcttacaa gtttttgtca acattgaaaa atgtcaagaa
     2641 aaaagacccg tcagctttta aagctgcata aaggcaagaa aaaagagggc ttttcgccct
     2701 cttttttttc tatttcctta ttttgacttg ggctttgcaa aaaacgaaac gacgagaccg
     2761 cagataacgg ctgccattat gccgacggag cctgcggtca agggtcccga caaggcgcct
     2821 ataaggcctt tttcgttgac ggcttttctt gttccctcgg caaggagatt gccaaagccc
     2881 gtcagcggca ctgtggcacc cgcccccgcc cattccttta agggctcgta aagtccgatc
     2941 gcgccgagga ttacgcctgt aataacatag ccgacaagca ctctgcccgg cgtcagcttt
     3001 gttttgtcaa ttatgagctg tccaatgacg cacagcagac cgcctacaat aaaagccttt
     3061 aagaacatca tttgtcctat tcactctttc tgctgtaata taaaaaatac ccttgatgat
     3121 gtcaaaggta ttttttgcag aatttattaa tttattcaat tccgagcggc cgctaatacc
     3181 agtattttcg gtcaagactc ctgtactgaa caccctccat aatatgcttt ttctcaatgg
     3241 tttctttacc gtcaaggtcg gcgatggttc tggcgacctt gagaattctc tcatacgctc
     3301 ttgcggagag gtcaagtctg tcaaatgccg atttcaaaaa ctctgcggcg tcgcttgaaa
     3361 gaacgcagaa ttttcttacc gctgcgctgt ccatgcgggc gtttgatgtg atttttgtgc
     3421 ccttaaatct ctccctttga agttttcttg ccttttcaac cctctcttta attgccgctg
     3481 agctttcttc gtttccctct cttgacaaat cgctgaaatc caccggcggg acttctatat
     3541 gtatgtccac cctgtcaagc agggggcccg aaatcttgga aagatatttc tgcaccgctc
     3601 ctttggggca ggtgcatttt cttttgggat gccccaaaaa accgcagggg caggggttca
     3661 tggcgcagac aagcatgact gagcaggggt atgagagggt gtaggaggct ctcgatattg
     3721 tgattacgcc gtcctccaaa ggctgtctta gggattctat cgccgccttt gaaaactcgg
     3781 gaagctcgtc caggaaaaga actccgttat gggcaaggct cagctcgccg ggcttcggag
     3841 ctgagccgcc tcccgacatt cccgccgccg ataccgtatg atggggcgag cggaagggtc
     3901 tcgttctgat taaggtatcc ttgcctttta aaagacccgc tatggagtaa acctttgtgg
     3961 attccagcgc ctcctcaaag ctcatgtcgg gcaggatgga gggcagcctt ttggcaagca
     4021 tgcttttgcc cgaaccggga ggtcctatca tgagtatgtt atgggagccg gcggcggcta
     4081 cctcaagggc tctttttgcc ttgtactgac ccttgacctg agaaaagtcg ggcaaagcgg
     4141 ataaatcctg cgcctgccta aactccacag gctccaaggg ttttaatgcg gttatgccgt
     4201 ttatgtggtc tactatttcc tccacgctct ctgcggggta gatggaaatg cccgttatcg
     4261 cggcgacctc tccctcgttt tctttaggga ggaaaacctc tttaattccc gcagcctttg
     4321 ccgcgagaac catggggaga gctccgctga tatgcctgag actgccgccc aaagacagct
     4381 cgccaatgaa cgccgtatcg gacaaatcgc cgcttatctg ctccgttgcc tttaggagag
     4441 ccataaaaat gggcaaatca tataaagggc ctgccttttt cacatcggcg ggggcaaggt
     4501 ttacggtcac atgggaaacg ggaaactcat agccgcagtt gttcaccgcc gccctgaccc
     4561 tttcttttga ttcgctgacc gccgcatcgg gcagacctac aatgtcaaaa cgcagtgggc
     4621 cagatgaaat gtccgcctcc acatcaacca taaaggcgtc aagacccatc agccccatgc
     4681 tgttaatatg tgagaacatg accttcctcc actgaattta taaaataata ttagtttatc
     4741 ggaggaaaaa attcaagcgt cattccaata aaaaaaggta tgccaagcag catacccgtt
     4801 atatttcaaa ttttattgtt ttcgtatttc atcaatctgt tcaaaattgc ttgccggagt
     4861 aataagcatc atgctaaggt ccattccccc ggcataagta gtagagattc ccggctttat
     4921 gcttctagta gcctttatat ttatatcaga aagaacaacc gacattatat atgccgtccc
     4981 ctgcacctcg ttttttaatg gccatttgcg gttgaacaaa aagaaaacaa caaaatcttg
     5041 agaatcaagc gaatgtagtt ttttcgctat ggtttccgcc ctttcctcaa cgggggcgtt
     5101 ggtcagacgc tcataaccgc ctcggaaaac tatgtatttc tcgggatggt ttttgccgtc
     5161 gccgtttctt ttaaaaattc ttgtgccgtg ggaactcctg cacacaatca gtgtttcgga
     5221 cattcgcttt aaaagtgcct tttcaatatc atgcgcagtc ttaaatttcc cgctttcata
     5281 atcgagggct acggatgcta aaggaactcc gtagcctaca gaaaaggttt tggagtctat
     5341 tacattgaca ttaattccgg cagaaacctt tcgtcttgaa aagagctttg cggcttttac
     5401 cgcctctccg tacatgccga aaaggttttt gtcgggcgtt acaatgaaga tgtcgctgca
     5461 cccgtcttcc tgcgcctcaa ttatggcatc caaataaacc tgaaacggaa caatgggttt
     5521 tacatcaagc gtaccgttct ctgcggtaaa tttgtcaagg gtaatattga tgctctcata
     5581 atatggcgcc ccgtcgataa tcatataaac aggaagcaat tttatgcacg caacttcttt
     5641 tttaattgac ggaagatcgc agccggtatc cgctataaat ctgacaagca ttcttcaacc
     5701 ttctttaagt tgacaaaagt ataaataata aagcattttg cgccattgaa ataacaacac
     5761 aaaatgttag aaacagaaaa catattaatg ccagacaatt agaagaagct acacaaaaaa
     5821 tgtagaatat tggtttaatt ataacataaa aggctttcag tcaattgtat acaaatgtgg
     5881 tttttttaca catttgcaaa ctttccgtag agggaaaggt ccggaaatcc ccattgtctg
     5941 agcacttcat aagctccgat tgcgacggaa tttgacaagt taagacttct ggcgtcgtta
     6001 atcataggga ttctgactgt tct
//
LOCUS       DAONVQ010000118         5979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_9894_length_5979_cov_4.982444, whole genome shotgun sequence.
ACCESSION   DAONVQ010000118 DAONVQ010000000
VERSION     DAONVQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9894_length_5979_cov_4.982444"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLD98_07490"
     CDS             complement(<1..584)
                     /locus_tag="PLD98_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQA06121.1"
                     /translation="MYLREPCYDIDELKKENLHIHTFYSSCAKPSMDIETIVRQAEKA
                     GLRRIAVTDHFNDPDFPVVEYNEEMRKKVKQLGAKVDVLFGAELSAYGIGKFLDRTEI
                     NKALDYRLYSCNHYHLDYWEHPEDKTPRGYAEHALKVVEALAKSGRADCVAHPFIGRF
                     VKIFEDRNLITNSITDNEIGDILTILKENNVAYEL"
     gene            complement(759..1430)
                     /locus_tag="PLD98_07495"
     CDS             complement(759..1430)
                     /locus_tag="PLD98_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HQA06122.1"
                     /translation="MKTSEGLIEFVKSKIGCPYWYGTFGNIASESLLKYKSQQYPKHY
                     TENREATYRSQFGKQVFDCSGLVKAYLWTDENGKIVYNSAQDLSANGFYKNCPVSGTI
                     STMPDLPGLLVFMPGHMGVYIGNGEVVEARGFSYGVVKTELLKRPWKNWGVCPWIQYK
                     GTGDIDVDNKLTAKDARLALRIVAGLEKADAVKKLLADIDGDGKVTAKDARMILQKVA
                     GLLEE"
     gene            complement(1588..3492)
                     /locus_tag="PLD98_07500"
     CDS             complement(1588..3492)
                     /locus_tag="PLD98_07500"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQA06123.1"
                     /translation="MNIRKFKFSKLFSLVFFLCLAIVFLILSPVAKVYNVEGYEIDTE
                     NLKPIQKGYKTAPIPVFMGSDKDLLPKSLSSYISLNEKIYYKSPSMTVFTDKNEKQSV
                     IAAAVKSSSGGAYGTGEIVVKKSEDNGLTWDSERTVFKLPVRQKPNSNKDYAAAFNSE
                     CLIFSADGEIILMTIMYPESKGPEDKTWIENADVYKQIGDVLYPVLYDEPSSVGKTIS
                     AAVPQNEYTVREDGFVYTPDGKKTAYYLPQFHSPEYAYQTMGDMYYCVGEPDYKTTPP
                     PLLPEYKPGTERDIYVGNIYLSENKPSFSLNEPIPVTKKKNGGIVETSPAPLRAPVTA
                     NIFVLKSRDGGESFSQPVNITYQIKGKADKGILSFASSSAVVLKNQVYYDKNGRIIVF
                     FKNSDGVFAVYSDNNADMFKRRQVVHFRNAEFSACFEDEEGSLISLSSKKKSNKVKAF
                     ESIDSGVKWNRDEPAGIPADGAALSALRLPVDYGKENSFKYADGMELGREYIIVAHSI
                     KDSGKDTAVLTIGALNPKDRISWNSEKKLNDDEFFSPNEKYNSFIGQSSLTVLENGNI
                     GILYESSPASLILFRQFSLEWIINGASPKIKTETPPLIILLMLAIGLSIILLTLYAAV
                     KALLKKCSSK"
     gene            3664..4533
                     /locus_tag="PLD98_07505"
     CDS             3664..4533
                     /locus_tag="PLD98_07505"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQA06124.1"
                     /translation="MWQRIVAFFTTLITLILSWFGINLGKVQTYRNVAYGTAARQVMD
                     VYYPDKPENIEGSVDAMLFIHGGAWSSGDKSVYASYCKKVAENGYVAATMNYRMFQQN
                     ATFVEMLEDINLAITKLKEKAEADGIEIDKIALMGASAGGHLSLLYAYTHYETSPLPI
                     AFVVGQVAPVDFLDPNFGETLGEHQLSLISYLTGVQIINENFSEHEDIIKAASPYYHV
                     TPDVPPTILCYGFKDTLVPYSNGVNLDAKLTEMGVEHTFLTYPNSGHGLENDKDVSER
                     FLEVLMEYAARFC"
     gene            complement(4616..4689)
                     /locus_tag="PLD98_07510"
     tRNA            complement(4616..4689)
                     /locus_tag="PLD98_07510"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4655..4657),aa:Gln,seq:ctg)
     gene            complement(4927..5289)
                     /locus_tag="PLD98_07515"
     CDS             complement(4927..5289)
                     /locus_tag="PLD98_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HQA06125.1"
                     /translation="MTPNKEDYLKQLYKLGAEGDMVSNKSLSHALGVSPASVTEMLTK
                     LKRDGYIELLPYKGSRLTERGAALAAELIKNHRLWEVFLVRILNMSPEEAHENAEMLE
                     HSTSAEVCSRLADFLGNP"
     gene            complement(5399..>5979)
                     /locus_tag="PLD98_07520"
     CDS             complement(5399..>5979)
                     /locus_tag="PLD98_07520"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06126.1"
                     /translation="SDRFLNIQGRICFRHSIYWGIASLAFTYLVHPFFLNNIFVHLPD
                     KYKTPLLTVILVIFAFDLINAVRAAMDIRKFMMKLTGLYDNIASTIGEIRNNVEQRLE
                     NVQNKITKQGMKLATWTAEVNQQLSDARKKFDKMQKEPENKDAGREEKKIYRILTRSS
                     ALRDSASRRMDYIQEILDDIIARIKDEDDEMY"
BASE COUNT         1466 a         1339 c         1364 g         1810 t
ORIGIN      
        1 agctcgtacg ccacattgtt ctctttgaga attgtgagaa tgtcgccgat ttcattgtcc
       61 gtaattgagt ttgtgataag gtttctgtcc tcaaagattt tgacaaatct gcctataaag
      121 gggtgggcaa cgcagtcggc tctgccggat ttcgcgaggg cctccaccac ctttaatgcg
      181 tgctcggcat agcctctggg ggtcttatcc tcggggtgct cccagtagtc aaggtgataa
      241 tggttgcagg aatagagcct gtaatcaagg gctttgttta tctctgttct gtcaagaaat
      301 ttgcctatac cgtatgctga aagctcggct ccgaaaagca catcaacctt ggcgccaagc
      361 tgttttacct tttttcgcat ctcctcgtta tactctacaa cggggaaatc ggggtcgtta
      421 aagtgatcgg ttacggcgat tctcctaaga cccgcctttt cggcttgtct tactatcgtt
      481 tcaatatcca tgctgggctt tgcgcatgag gaatagaagg tatggatatg caggttctct
      541 tttttaagct cgtcaatgtc atagcagggt tctctgagat acataagata gctccttctg
      601 tttacttcgt cgcaatatgg attaattata gcacaagcct ttaaagcaaa acaataaaaa
      661 ggacaaaaag ctcaaagttt ttatcgcggt tttttgctct gcgcaaaaac aaaaacagcc
      721 gaaagactcg gctgttttta atgggtttta atttaccgtt actcttccaa aagacccgca
      781 accttttgca aaatcatgcg ggcgtcctta gcggttactt ttccgtcgcc gtcaatatcc
      841 gcaagcagtt ttttgaccgc gtctgccttt tcaagtcccg ccacgattct gagagcaagt
      901 cttgcgtctt tggcggtaag cttattgtcc acatcgatgt cgcctgtgcc cttgtactgt
      961 atccacggac atacgcccca gttcttccac gggcgtttaa gcagctcggt ttttacgact
     1021 ccgtagctga agcctcttgc ctcaacaact tcgccattgc cgatataaac tcccatgtga
     1081 cctggcataa acacaaggag tccgggcagg tccggcatgg ttgaaatagt tcccgatacg
     1141 gggcagttct tgtaaaaacc gtttgcgctt aaatcctgag cgctgttgta tacgatttta
     1201 ccgttttcgt cagtccacag atatgcctta accaaacccg aacagtcgaa aacctgcttg
     1261 ccgaattggg aacgataagt agcctctctg ttctccgtat aatgcttggg atattgctga
     1321 gatttatact taaggagcga ttcggatgct atatttccaa atgtaccata ccaataggga
     1381 cacccaattt tagattttac gaattctata agtccttcac ttgttttcat atatttcatc
     1441 ccttccaatg tagaattcga gtggtaagag tataaaaata aagatttaaa caatttgcct
     1501 gtctatatac agtttataca ctgaatgaat ttaagtgcct ttataatttc aagtcccaaa
     1561 tttaagaccc tccgtttctc ggagggtcta tttgcttgag cactttttta gcagggcttt
     1621 gaccgccgcg tacagagtta aaaggataat tgaaagacct atcgcgagca ttaaaagaat
     1681 tattagcgga ggggtttcgg tttttatttt tggcgacgcg ccgtttatta tccactcgag
     1741 gctgaactgt ctgaaaagga tgagtgaggc cggcgatgat tcgtaaagaa taccgatatt
     1801 gccgttttca agaacggtta gagagctttg accgataaag ctgttgtact tttcgttcgg
     1861 ggagaagaat tcatcgtcat tcagtttttt ctcggaattc cagcttattc tgtccttggg
     1921 atttaaagct cctatggtga gaacagcagt gtctttgccc gagtctttta tgctgtgggc
     1981 gactatgata tactccctgc ccagctccat tccgtctgcg tacttgaagc tattttcctt
     2041 gccgtagtct acggggagcc tcagcgccga caaggcggcg ccgtctgccg ggattcccgc
     2101 cggctcgtct ctgttccatt tgaccccgct gtctatgctc tcaaaagctt ttactttgtt
     2161 gctcttcttt tttgaggaaa ggcttattaa gctgccttcc tcgtcctcaa agcaggcgga
     2221 aaactcagca tttctgaaat gcaccacctg ccttcttttg aacatatcgg cgttattgtc
     2281 gctgtaaacg gcaaagacgc cgtcggagtt tttgaaaaac actattattc tgccgttctt
     2341 gtcgtaataa acttggtttt tgagaacgac agcggagctt gaggcaaagc tgagaatacc
     2401 cttgtccgcc ttgcctttaa tctgataggt gatattgacg ggctgggaaa agctctctcc
     2461 accgtcgcgg cttttcagaa cgaaaatatt tgctgtaacg ggagcgcgca aaggcgccgg
     2521 ggaggtttcc acaatgccgc cgtttttctt ctttgtaacc ggtatgggct cattaagact
     2581 gaaactcggc ttattctcgc tgagataaat gttccccaca taaatgtccc gctcggtacc
     2641 cggcttatat tcgggcaaaa ggggcggagg ggtggtctta taatcaggct ctcccacgca
     2701 gtagtacata tcgcccattg tttggtaggc gtattcgggg gaatgaaact gagggagata
     2761 ataggcagtt ttcttgccat cgggggtgta gacaaagccg tcctcgcgga ctgtgtattc
     2821 gttctggggc actgccgcgc ttatggtttt gcccacgctt gaaggctcgt cgtaaagcac
     2881 gggatataaa acatcgccaa tttgcttgta gacatccgcg ttttcaatcc atgttttgtc
     2941 ctcgggaccc ttgctttcgg ggtacattat agtcatcaga ataatctcgc cgtcggcgga
     3001 gaaaatcaga cactctgaat tgaatgcggc ggcatagtcc ttgtttgaat tcggtttttg
     3061 tcttacgggg agtttaaata ctgtgcgctc gctgtcccat gtaagaccgt tgtcctcact
     3121 tttctttaca actatttcgc cagtgccgta tgcgccgccg ctgctgcttt ttacggctgc
     3181 ggcgattacg gactgctttt cgttcttatc ggtaaatacg gtcattgagg ggcttttgta
     3241 gtaaatcttc tcattcagcg atatatagct tgacaaggat ttggggagta aatccttgtc
     3301 actgcccatg aatacgggga ttggggcggt tttgtagccc ttttgtatgg gctttaaatt
     3361 ttcggtgtct atttcatagc cttccacatt atagaccttc gcgacaggac ttagaattaa
     3421 gaaaacaatt gcaaggcaga ggaaaaagac aagtgaaaac agctttgaaa atttaaattt
     3481 ccgtatgttc attgccttca ccccgccgcc aaaaaattag ttgcaaaatt attattgctc
     3541 ttgtaaagtc ccttatcctg cttataaaaa caggcggcaa gagaaaatct gccataattc
     3601 cataaaattt acttgatatt tgacttttga gaatataaac tggaataaaa agggggaaga
     3661 aatatgtggc aaagaatagt tgcgtttttt acgacgctca ttactttgat tttgtcttgg
     3721 ttcggcatta atctgggcaa agttcagacc tacaggaatg tggcatacgg cactgccgca
     3781 aggcaggtta tggatgttta ttatcccgac aaacccgaaa acattgaggg ctctgtggac
     3841 gcaatgctgt tcatccacgg cggagcatgg tcatccggcg acaagagcgt atatgcaagc
     3901 tactgcaaga aggtcgctga gaacggctat gttgccgcta ccatgaacta cagaatgttc
     3961 cagcagaacg ccacatttgt tgaaatgctt gaggatatta acctcgccat cacaaagctt
     4021 aaagaaaagg cggaagccga cggtattgag attgacaaaa tcgctcttat gggcgcctcc
     4081 gctggcggtc atctctccct gctttacgct tacacacatt atgaaacctc ccctctgccc
     4141 atagcctttg ttgtaggtca ggtagctccc gtagacttcc ttgacccgaa ttttggtgaa
     4201 actctgggag agcatcagct ttcactcatt tcttatctga cgggcgtaca aattataaat
     4261 gaaaacttta gtgaacacga agatattatt aaggctgcgt cgccttacta tcatgtaacc
     4321 ccggatgttc cgcccacaat actctgctac ggttttaagg acactcttgt accgtattca
     4381 aacggcgtaa atctggacgc gaaacttact gaaatgggag ttgagcatac attccttact
     4441 tacccgaact ccggtcacgg tcttgaaaac gataaggatg taagcgaaag attccttgag
     4501 gttctaatgg aatacgccgc aaggttctgc tgaataaaaa aatttcgccc gacacaatgt
     4561 cgggcgttat taatgcaaaa gaaaagccct gaccgatcgg tcagggcttt tgccgtggct
     4621 gcggaactag gattcgaacc cagacaaaca gagtcagagt ctgccgtgct accgttacac
     4681 aattccgcat tactcgcttt cggcataggt aattataatg agatttaaat gttttgtcaa
     4741 ggactaattt tatataattt tgcgtttctt taataaatca tagcaggtct ttcgcaggat
     4801 atattatatt gtgtttttct atttttattc ctgtccaaaa ttatgtaaat tagcttattg
     4861 ggtgctttta ttgggacaga gccgtataaa cggcaaatag tccaatcagc tttccgagta
     4921 aagtttttag ggattgccga ggaaatcagc cagccttgag caaacttcgg cgcttgttga
     4981 gtgctccagc atttctgcgt tttcgtgggc ttcctcgggg gacatattta gaattcttac
     5041 aaggaaaacc tcccaaagcc tgtggttttt aataagctct gccgccaaag ccgctcccct
     5101 ttcggtaagc cttgagccct tgtatggcaa aagctctata tagccgtctc gcttgagttt
     5161 cgtgagcatc tctgtcaccg acgcgggtga aacgccaagg gcatgtgaga ggcttttgtt
     5221 gctgaccata tcgccttcag cgcccagttt ataaagctgt tttaaataat cctctttgtt
     5281 gggagtcata aatcctcctt ttttgtttct aaactaatat tatgataatc gatgattatt
     5341 tgttcttcaa gcttttgctt tcgggcacaa aaaaaccgcc gttcggcggt tttctgtttt
     5401 aatacatttc atcgtcttcg tctttaatgc gggcgattat gtcgtcaagg atttcctgaa
     5461 tatagtccat tctgcggctg gcggagtccc ttaacgctga ggagcgggta aggattctgt
     5521 aaatcttctt ttcctctctg cccgcgtcct tgttctccgg ctctttttgc atcttgtcga
     5581 atttctttct cgcgtctgac agctgctggt ttacttccgc ggtccatgtg gccagcttca
     5641 tgccctgctt tgtgattttg ttctgaacat tctcaagccg ctgctccaca ttgtttctga
     5701 tttcgccgat tgtggaggct atgttgtcgt agagtcccgt aagcttcatc atgaactttc
     5761 ttatgtccat ggccgccctg acggcgttga taaggtcaaa ggcgaaaatc acaaggatta
     5821 cggtaagcag aggggtttta tatttatcgg ggagatgaac gaaaatgtta tttaagaaga
     5881 aggggtgaac aaggtatgta aaggcaaggc tcgctatgcc ccaatagatg gagtgtctga
     5941 agcagattct tccctgtatg ttaaggaatc tgtcggaat
//
LOCUS       DAONVQ010000119         5800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_10240_length_5800_cov_4.418451, whole genome shotgun sequence.
ACCESSION   DAONVQ010000119 DAONVQ010000000
VERSION     DAONVQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10240_length_5800_cov_4.418451"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..931
                     /locus_tag="PLD98_07525"
     CDS             323..931
                     /locus_tag="PLD98_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQA06127.1"
                     /translation="MKKFYKLIAIILTIVMSFSVMSLAAFADEPTTEGTATEVQKDEE
                     MVDILLRFKELIERFINGVKKVIEEIRNFLGSVKDITKVKVDSISFPNEATVLKVGDT
                     ERLVPTILPEKATNKNVTYSSSDESVATVDENGVVTAVGTGTATITATTEDGGKTAAK
                     HIVVGDIVVKENESIYDAYLDAEEGQVIAVAPGVYKERLVIS"
     gene            1130..1879
                     /locus_tag="PLD98_07530"
     CDS             1130..1879
                     /locus_tag="PLD98_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06128.1"
                     /translation="MYAHGSDLQIKNNVFDGFNYVQLYVSSAVYNYSNGTWDYHSEYV
                     NNLLLENNLFKDSPSSYYNVQLQGVSCTIRGNVVDNASGIIIQPYHNAGGGLVENNIV
                     NTFVLGFWHNTAYLGSGKWEYKNNVINAVAVPENMTTPPTTYVRWESFLIQSFGVGGA
                     WNTGAAPEVVYENNVVDATNASPELISYGVNFIAPVTNDAKAIFTNNTFAGVQIGVDR
                     KAGALDLNEMLANNAFPEGSAVIGNQIKIAE"
     gene            2004..3158
                     /locus_tag="PLD98_07535"
     CDS             2004..3158
                     /locus_tag="PLD98_07535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQA06129.1"
                     /translation="MIVRRLTDSDALAQESVASSAFIYAFDPEKVEKLEHDYMLGAFN
                     DEGVMMACLEVYDYKNYFGKGTLGCAGVGGVASKPEFRRGGAVRAMFNYLFEDAPKQG
                     WDISILYPFSEEYYQKFGYSAISNKMYLEADFRCLEQIPRCSGAVLYDGSQSEALYEL
                     YNKIARKYSLAFMRNSDKYFNTNYPKDLKFTYLWKNDKGEFKAYVNCEFNRAESKVIV
                     QEIGFIDKEGMLGILGFLRVFDGNYKTIVFHNLPQDTPIYPLLTNLSMVTRKLINNGS
                     VRVFNLKKVLELNVYPKEKGSFVFESTDEITKGVYSVKYENSAAEISVSESGKPDFIM
                     DSIAISRYIVGGVSGGFEALSYDSHIEVVNENEDFLKAFPKRNTFFTDGF"
     gene            3178..4512
                     /locus_tag="PLD98_07540"
     CDS             3178..4512
                     /locus_tag="PLD98_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06130.1"
                     /translation="MNGITGWDYHPYVPYNEIYRKGEPYISMLAPSENALTFQWQSGA
                     GENGAKVFYRKRGEGEFKEQNIDASRVELTSLEQGAEYEFYVQDKLGRKSSVRLFETG
                     YVPGNVINYLHPEDSAYEFSGRYLCSPSIVRVGPSTLIASCDLFKSRAPQNLSLIFRS
                     DDNGKTWSNIAELMPCFWGRLFVHKGALYMLSTSTEYGDLLIGRSDDLGRTWTAPTVL
                     ARGSSHCEESGFHKAPVPVVNIRGRLYSAVEYGCWHKKRYANVVISIDENADLLKAEN
                     WTISAPAIFDLNWEGALKGHCPAAIEGNIVETPYGGIVNILRYAEGKALMLKADEQNP
                     EKAPEFVSFIDFPFAHTKFEIMKTEKGYFACGNRAPRRNVLSLAHSDDLIHWTIVKDL
                     INYSHLSSDFTAFQYPTAFIEGDKLIVVSRTAFNKCANFHDSNYITFHEFSI"
     gene            4678..>5800
                     /locus_tag="PLD98_07545"
     CDS             4678..>5800
                     /locus_tag="PLD98_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06131.1"
                     /translation="MQKAYISKFKRMLKVLSAAVLLITAVGSFNFAAFYGAESRAFSN
                     SAPVLNIEDAFLDLSKPAELTDTLVGSATVAATQKTESIAKSTAPFVNTDSSNSDNTS
                     NPVLTPIQKVGSSIANFSAPLPIKTGNKIKGMLASKSDNMVYSLEISQRGYIVLELSH
                     SLPSAPANAWNAVLYEEYNSTGIGGEIKYRPLGRVSSTLKDTDVATSEKIGVYPGNYI
                     IVVSSGTAFSPTEFYIKADFTATFDYEAGYNDTITRYNEITLNKPIKGVMNEGEQPDS
                     DWFMFRVDKKGFVRISFEHEDLKLPQVGWKIYLYDQWENELYYAKSNFNDTLVRSGLI
                     GLDEGYYFVNISQHIHSEAEYTLTVSFEQSNLAESEYNDS"
BASE COUNT         1733 a         1266 c         1288 g         1513 t
ORIGIN      
        1 aaatctaaaa caaattaagc ggttcgtaat gaaccgcttt tttgtttgcc taaattttag
       61 attaataatt ggaaaaattt tattattttc tttttttata acctggttat cataaagaca
      121 gcaaaagttt ttgcaatagt ttgttgaatt tattaaatag ctttaaattt ttgtgaacct
      181 taaatatatt ataaactttg tgtgtcgatt ttgacgaaaa gtcaataagg gcactgataa
      241 atcaagaaaa ttatagttgc gaaaaaacgc agttttatat ataatcttgt ctatctcaga
      301 aaaagacaag gagatgaaag aaatgaaaaa gttttataaa ttaattgcta ttattttgac
      361 aattgtaatg tctttttcgg ttatgagttt agctgcattc gcagatgagc ccactactga
      421 aggcacagca actgaagttc agaaagatga agagatggtt gatatccttc ttaggttcaa
      481 agagcttata gagaggttca ttaacggcgt caaaaaggta attgaggaaa taaggaactt
      541 cttaggttca gttaaagata taacaaaagt aaaagttgat tccatcagct tcccgaatga
      601 agctaccgta cttaaagtcg gggacacaga gagacttgtt cccaccattc ttcccgagaa
      661 agcaactaat aagaatgtta catattcttc cagcgacgag agcgtcgcaa ccgttgacga
      721 aaacggcgtt gtaacagccg ttggaacagg cacagcaaca attacagcca caacagagga
      781 cggcggcaaa accgctgcta agcacatcgt tgtcggcgac atcgttgtaa aagaaaatga
      841 aagcatttat gatgcatatc ttgatgctga ggaaggtcag gttattgctg tcgctcccgg
      901 agtttacaaa gaacgacttg taatcagcta gagagtttcc ttccttggag caaacgcagg
      961 catttccgcc aacactcagc gcggcgctga gactgttctt acatttgacg agagccacat
     1021 tacaggctca tccgtaactc tttttaacaa cagcgcttcc ggcgttgttt tcgacggttt
     1081 ctatttctac gacggcggct acgcatcgcc ctctcagctt gtcagagcaa tgtatgcaca
     1141 cggttccgat ttgcagatta aaaataatgt cttcgacgga tttaactatg ttcagctcta
     1201 tgtttccagc gccgtttaca attatagtaa cggcacttgg gactatcact cagagtatgt
     1261 aaacaatctc cttcttgaga acaacctttt taaagattct ccgtcctcat actacaatgt
     1321 tcagctccag ggcgtttcct gcacaatcag aggcaatgtg gttgacaacg cttccggcat
     1381 catcattcag ccctaccaca acgcaggcgg cggacttgtt gagaacaaca tagtcaacac
     1441 cttcgtgctt ggcttctggc acaacacagc atatcttggc tccggcaagt gggagtacaa
     1501 gaataatgtt atcaacgcag tggccgttcc tgaaaacatg accacaccgc ccacaaccta
     1561 tgtaagatgg gagagcttcc tcattcagtc cttcggcgtt ggcggagctt ggaacacagg
     1621 cgcagcaccc gaggttgtct atgagaacaa tgtggtcgac gctacgaacg caagtcccga
     1681 gcttatcagc tacggtgtaa acttcatagc acccgtcaca aatgacgcaa aagcaatttt
     1741 cacaaacaat actttcgccg gtgtccaaat cggcgttgac agaaaagcag gcgcccttga
     1801 ccttaatgaa atgcttgcaa acaacgcctt ccccgagggc agcgctgtaa tcggcaatca
     1861 gattaagata gctgagtaat ataacacccc aattgcaccc atgcaattgg ggtcttttta
     1921 tgccctttaa aattattgtt tgaagtttaa tcccgctttg atataataat ataaataact
     1981 attacagaat tggggagtgg actatgatag ttagaagact taccgacagc gacgcactgg
     2041 cacaggaaag cgtagcgtcc tcagcgttta tttacgcctt tgaccctgaa aaagttgaaa
     2101 aactggaaca cgattatatg ctgggcgcct ttaacgatga aggcgttatg atggcttgtc
     2161 ttgaggtgta cgattataag aattatttcg gcaaaggaac tttgggctgc gcgggcgtgg
     2221 gcggagttgc ctcaaagcct gaattcagac gcggcggagc cgtaagagcc atgtttaatt
     2281 acctttttga ggatgctccg aagcagggct gggatatttc aatcctttac cccttctccg
     2341 aggagtatta tcaaaagttc ggatactcgg caatctcaaa caaaatgtat ctggaagcgg
     2401 atttcagatg ccttgagcag attcccagat gctccggcgc ggtgctctac gacggctctc
     2461 aaagcgaggc tctctatgag ctctacaata agatagcgag aaaatacagc cttgctttta
     2521 tgagaaacag cgataagtat ttcaatacca actaccccaa ggacttaaag tttacatacc
     2581 tttggaagaa tgacaagggc gagtttaagg catatgtaaa ctgcgagttc aacagagccg
     2641 aaagcaaagt aatcgttcag gaaatcggct ttatagataa agaaggaatg ctgggcatct
     2701 tgggcttttt aagagtattt gacggcaatt acaagacgat tgtattccac aacctgcccc
     2761 aagacactcc tatttatccg ctgctgacaa acctctccat ggtaacgaga aagctaataa
     2821 acaacggttc ggtcagagtc tttaacctta aaaaggtgct ggagctgaat gtctatccaa
     2881 aagaaaaagg cagctttgta tttgagagca ccgacgagat tactaagggc gtctacagcg
     2941 taaaatacga aaactccgca gcggaaatca gcgtttcaga aagcggaaag cccgatttta
     3001 ttatggattc aatcgcaatt tcccgatata ttgtcggcgg cgtcagcggc ggatttgagg
     3061 cgctgtcata tgactcgcat attgaagtcg taaacgagaa tgaggacttt ttaaaggcat
     3121 tcccaaagag aaataccttc tttacagacg gtttctaaca gaaaggtaag gaaaatcatg
     3181 aatggcatta caggctggga ctatcaccct tatgttccct ataatgaaat ctatagaaaa
     3241 ggcgagcctt atatctccat gcttgcaccc tccgaaaacg ccctcacatt ccagtggcaa
     3301 agcggcgcgg gggagaacgg cgcaaaagtt ttctacagaa aaaggggaga aggggagttt
     3361 aaggaacaaa acattgacgc aagcagggtt gagttgactt ctcttgaaca aggcgcagaa
     3421 tatgagtttt atgttcagga caagctgggc aggaaaagca gcgtaagact ttttgaaaca
     3481 ggctatgtcc cgggaaatgt tataaactat cttcacccag aagattccgc atacgagttt
     3541 tcgggcaggt acctttgcag cccgtcaatc gttagggttg ggccaagcac cctcatagcc
     3601 tcctgcgacc tttttaaatc cagagcgccg cagaatttaa gccttatttt ccgctccgat
     3661 gacaacggaa aaacatggag caatattgcg gagttaatgc cctgcttttg gggaagatta
     3721 tttgtccaca agggagcttt atatatgctc tctacctcta cggaatacgg cgatttgctt
     3781 atcggacgct ccgatgattt gggcaggacc tggacagccc cgacggtcct tgcaagaggc
     3841 tcatcacact gcgaggagag cggttttcac aaggcgcccg tacccgttgt aaatattaga
     3901 ggcaggcttt actccgctgt ggagtacggc tgctggcata aaaaaagata cgccaatgtc
     3961 gtcatttcca ttgacgaaaa cgccgacctt ttaaaggcgg aaaactggac aatctctgcc
     4021 ccggcgattt ttgaccttaa ctgggagggc gcgcttaaag gccactgccc agcagcgata
     4081 gagggcaata ttgttgaaac cccctacggc ggcattgtga atatcctcag atacgccgaa
     4141 ggaaaggctt taatgctgaa agcagatgaa caaaaccccg aaaaggctcc cgaatttgtg
     4201 tcatttattg atttcccatt tgcccataca aagtttgaga taatgaaaac agaaaagggt
     4261 tactttgcat gcggcaaccg cgcaccgcgg cgcaatgtcc tctcccttgc ccattcagac
     4321 gatttgattc actggaccat agttaaggat ttaattaatt acagccacct ttcatccgat
     4381 tttacagcct ttcaatatcc caccgctttt attgagggcg ataagctgat agtagttagc
     4441 agaaccgcct ttaataagtg cgctaacttc cacgacagca actatataac attccacgaa
     4501 ttcagtattt aataaagaaa cttgctccga taggggcaag ttttttgttg caggttttaa
     4561 cactaatggc aaaaaattta taaaacgcat tcactgtaaa tattgaacga agaaataaac
     4621 tgtgataaac ttaatttgtc gaaatgtatt cgagtttatt gcaagaggat ttgtaaaatg
     4681 caaaaggctt acatatcaaa attcaaaaga atgttaaagg ttctatctgc ggctgtttta
     4741 cttattacgg ctgtgggcag ctttaatttt gccgcatttt acggcgctga aagccgcgca
     4801 ttttcaaatt ccgcgccagt tttaaatatc gaagacgctt ttcttgattt gtcgaagccc
     4861 gctgagctta ccgatacgct ggtagggtca gcaacggtcg cggctactca aaagacggaa
     4921 agcattgcaa aaagcactgc gccttttgta aacaccgatt cttcaaattc agataatact
     4981 tcaaatcccg tacttactcc cattcaaaag gtaggctctt ccatcgcaaa tttcagcgcc
     5041 ccgctgccca ttaagacggg caacaaaatc aagggcatgc tggcttccaa gtcggacaat
     5101 atggtttaca gcctggaaat cagccagaga ggatatattg tgctggagct ttcgcacagc
     5161 ctccccagcg cgcccgcaaa cgcatggaac gcggtgcttt atgaggagta taactccacg
     5221 ggcattggcg gcgagattaa ataccgcccc ctcggcagag taagctccac tttaaaggat
     5281 acagatgtcg caacatccga aaaaatcggc gtttaccccg gaaattacat tatagttgta
     5341 agctcgggta cggctttttc tccgactgag ttttacataa aagcagattt cacagccacc
     5401 tttgattatg aggcaggcta taacgacacc attacaagat ataacgaaat aaccctcaat
     5461 aagcccatta agggagttat gaatgagggc gagcagcccg actccgactg gtttatgttc
     5521 agggtcgaca aaaagggctt tgtgagaatc tcctttgagc atgaggattt aaagctcccc
     5581 caggtcggat ggaaaatcta tctctatgac cagtgggaga atgagcttta ctacgcaaaa
     5641 tccaatttca acgacacgct tgtcagaagc ggtcttatcg gtcttgacga gggctactat
     5701 tttgtaaata tttctcagca tattcactcc gaagcagagt acactttaac cgtaagcttt
     5761 gagcagtcaa atcttgctga gagcgaatat aacgacagta
//
LOCUS       DAONVQ010000120         5697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_10453_length_5697_cov_4.394009, whole genome shotgun sequence.
ACCESSION   DAONVQ010000120 DAONVQ010000000
VERSION     DAONVQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10453_length_5697_cov_4.394009"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2925
                     /locus_tag="PLD98_07550"
     CDS             <1..2925
                     /locus_tag="PLD98_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HQA06132.1"
                     /translation="LGDDWAATYDCAKIYTASVEIPESYKNAKVYLEIDFGGEALVKL
                     NGWIVGSLSSTMNRGWKSRDTIIVDSKILESGVLNIELELAVDSAGFCDYAMDGAKSI
                     TYNFAKARLCRVDEECEAFIFDTDLLLDSFKAAEDEYIKESLYKVLDDSIHMVDFDFD
                     DSEVRQSIYKAREYLNSELKKIKFTPQSEVVMQGHSHIDIAWLWRIQETERKAARTFA
                     NNLSLMDKYPEFKCAQSQAIVYDMTKRIYPALYDRIKEKVKNGQWEIVGNVWVEADTN
                     IASGESLIRQLLYGREFFLKEFGVVSDTYWLPDCFGFSWALPQIIRRSGMKYFITAKL
                     CGQDTNRFPYTSFVWKGHDGSEIVGYLARTHYQGDYTPSDLMKAQKENDQRDVFRKAF
                     GMYGYGDGGGGTTYAMLERAKRLKAYPGLPSSKTGTAGEFFDELNKVRDELPVINDEL
                     YYENHRGTYTSQGFIKKGNRKGEILLCEAESASLIASALSGFEYDKDRLESVWKILLT
                     NQFHDILPGTSIHEAMEDCRPAYEKIYSEGGEIKLSALKAVSSNIGSPAEGVAVWNLS
                     NHTVSSEVVIDTDGRYLRAADGSEPIQTLTADGKLKMLAEDIPPMGYKVYLYSADPLG
                     AKGGVKVTTRLLENDCLRVELDENGIITSIYDKVNSRETLKGCGNLLSVFQDKCVHET
                     AWNLELNYKKKRWDLIKAESIEIKEQNDVCGIITIRRRFNRSEIVQDITLRANENHVE
                     FDTYVNWQERDKILKAAFDVDILNTYASFEIAHGAIKRPNHANNSYDKARFEQCGHKW
                     ADLSEGDYGVSLITDCKYGYDVFGSVMRISLLRAPNCPDRTADSGEHRFKYAYYPHKY
                     TWQLGGTVQKAITFNKELCAVEVGKNGGKLPVSYSFAETSGKYVVLDALKKAQDGNGY
                     ILRVYEPEQSRGTVKITLNLPFSKVTECNLMEVDERDIETDGNSFSFEIKPFEVRTFR
                     IR"
     gene            3257..3439
                     /locus_tag="PLD98_07555"
     CDS             3257..3439
                     /locus_tag="PLD98_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06133.1"
                     /translation="MSKSLIWSVLSLVVAASLIYGFVVTVFSEGGPFGPEKPSSAVEA
                     EAEANDAGNLPDVIFS"
     gene            complement(3579..>5697)
                     /gene="mfd"
                     /locus_tag="PLD98_07560"
     CDS             complement(3579..>5697)
                     /gene="mfd"
                     /locus_tag="PLD98_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQA06134.1"
                     /translation="KVLIISFGKRAEEKRRGKNTGFKISEGFNSLEELVKGDYVVHSV
                     HGIGIFDGIQKIETGGVVKDYIKIKYDKNDVLYVPVTQLDLVSKYIGPYEGARQVKLS
                     RLGSAEWEKTKNRVRKSVKDMAKQLLALYAKRVSTKGFAFSKDIDMQNDFERRFEFEE
                     TEDQLRCVNEIKNDMEQPYPMDRLLCGDVGFGKTEVALRAAFKCVADGKQCAILVPTT
                     ILALQHYQTILKRFEGFPITAEMLSRFRTPKEQEAIKKGLKRGSIDIVVGTHSIIANS
                     VEFKDLGLLIIDEEQRFGVEQKEKLKERFPAVDCLTISATPIPRTLNMAMSGIRDMSI
                     IEEAPQDRQPVQTYVLEHDMGILAEAMAKELRRGGQVYYVHNRIETIERTALRIKEYL
                     PDARIEIAHGKMSESVLSDIWRRLIEGEIDILVCTTIIETGVDVPNVNTLIIENADHM
                     GLAQLHQMRGRVGRSSRRASAYFTFVKGKELSETAYRRLSAIREYTEFGSGFKIAMRD
                     LEIRGAGDLLGASQHGHMEAVGYDLYLKLLAQEVSKEKGEETEVKTEECLIDLQVDAH
                     IPEKYIESVPQRLSIYRRIAEIKNQDDADDVLDELTDRFGDPPDAVMGLIKISMLRNA
                     AARLGIYEVGQRGDSLLLFTPETDMTMVSKLANGLKGRIFVSAAKKPYISVRRLKNQG
                     PLEALEEIINVLTKPEPEGEKKE"
BASE COUNT         1470 a         1333 c         1433 g         1461 t
ORIGIN      
        1 ctgggcgatg attgggcggc aacatatgac tgcgcaaaga tatacaccgc aagcgtggaa
       61 atacccgaaa gctacaaaaa cgccaaggtt tatcttgaaa tcgactttgg cggcgaggcg
      121 cttgtgaaat taaacggctg gattgtgggt tctctcagct ccacgatgaa cagaggctgg
      181 aagtcaaggg ataccattat cgttgactca aagatccttg aaagcggcgt tttgaatatt
      241 gagcttgagc tggcggttga ctccgcgggc ttttgcgatt atgccatgga cggcgctaag
      301 tcaatcacct acaacttcgc gaaggcaagg ctctgcaggg ttgatgagga gtgcgaggct
      361 tttatttttg acaccgattt gcttcttgat tctttcaagg ctgcagagga cgagtacata
      421 aaggaaagcc tctacaaagt ccttgacgac agcattcata tggttgactt tgattttgac
      481 gacagcgagg ttaggcagtc catatacaag gcaagggagt atctgaactc cgagcttaaa
      541 aagattaaat ttaccccgca gagcgaggta gtaatgcagg ggcactctca cattgacatt
      601 gcgtggctgt ggaggattca ggagacagag cgcaaggccg cgagaacctt tgccaataat
      661 ctgtcgctca tggacaaata ccccgaattt aagtgcgctc aaagtcaggc tatagtttac
      721 gacatgacaa agcggattta ccccgccctt tacgacagga taaaggagaa ggtcaaaaac
      781 ggacagtggg aaattgtagg caatgtctgg gttgaggcgg acaccaacat cgcgtctggt
      841 gaaagtctta taaggcagct tctctacggc agggagttct ttttaaagga attcggcgtt
      901 gtttccgaca cctactggct ccccgactgc ttcggctttt catgggctct gccgcagatt
      961 ataaggcgca gtggcatgaa gtattttatc accgcaaagc tgtgcggtca ggataccaac
     1021 cgcttcccct atacttcttt tgtctggaag ggtcacgacg gttctgaaat tgtgggctat
     1081 cttgcccgca cccattatca gggagattac actccgtcag atcttatgaa ggctcaaaag
     1141 gaaaacgacc aaagggatgt tttccgcaaa gccttcggaa tgtacggcta cggcgacggc
     1201 ggcggaggca ccacctacgc catgcttgaa agagcaaaga ggctgaaagc ttaccccggt
     1261 ctcccctcct ccaaaacggg cacggccggc gagttttttg acgagctgaa caaagtgcgt
     1321 gacgagctgc ccgttattaa tgatgagctt tattacgaga atcacagagg tacatataca
     1381 agtcagggct ttataaaaaa gggcaaccgc aagggagaga ttctgctttg cgaggctgag
     1441 tcggcgtctc tgattgcctc cgctctgtcg ggttttgaat acgataagga tagacttgaa
     1501 tccgtctgga aaatcctcct tacaaatcag ttccatgaca ttctccccgg cacttcaatt
     1561 catgaggcta tggaggattg cagacccgct tatgaaaaga tttactcgga aggcggcgag
     1621 attaaactca gcgcgctgaa ggctgtaagc agtaatatcg gcagccccgc agagggcgtg
     1681 gcggtgtgga atctttcaaa ccacactgtt tcgtctgaag ttgtaattga tacagacggc
     1741 aggtatttaa gagcggctga cggctcagag ccgattcaga ctcttaccgc agacggaaaa
     1801 ctgaaaatgc ttgccgagga tattccgccc atgggctata aggtttacct ttacagtgcg
     1861 gacccactcg gcgcaaaggg cggcgtaaag gttaccacaa ggcttttgga gaatgattgc
     1921 ctcagagttg aactcgatga aaacggcatc ataacaagca tttatgacaa ggtaaattcg
     1981 agggaaacat tgaagggctg cggaaacctt ctaagcgtat tccaggacaa atgcgtgcat
     2041 gagaccgcgt ggaacttaga gcttaactac aaaaagaagc gctgggattt aattaaggct
     2101 gaaagcattg aaattaagga gcagaacgat gtctgcggaa tcatcactat tcgcagaagg
     2161 ttcaaccgct ccgagattgt tcaggatata actctcaggg caaatgaaaa ccatgtggag
     2221 tttgacactt atgtaaactg gcaggagcgg gataagatac tcaaagccgc ctttgatgtg
     2281 gatattctta atacttatgc cagctttgag atagcccacg gagccataaa gagacccaat
     2341 cacgcaaaca acagttacga caaggcaagg tttgagcagt gcggacacaa gtgggcggac
     2401 ctttcagagg gcgattacgg cgtgagcctc ataaccgact gcaagtacgg ctacgatgta
     2461 ttcggcagcg ttatgaggat aagtctgctc agagcgccca actgccccga cagaaccgcc
     2521 gacagcggtg agcacagatt caaatacgcc tactatcccc acaaatacac ttggcagctc
     2581 ggcggcacag tacagaaggc tattaccttt aacaaggagc tttgcgcggt agaggtcggc
     2641 aaaaacggcg gcaaactgcc cgtatcctac tcatttgcag aaacaagcgg caagtatgtg
     2701 gttctggacg ctcttaaaaa ggctcaggac ggaaacggct atatcttacg agtttatgag
     2761 cccgaacagt caaggggtac agttaaaatt actttgaatc tccccttctc aaaggtcacc
     2821 gagtgcaatc tgatggaggt tgacgaaagg gatattgaaa ccgacggcaa ttccttcagc
     2881 tttgaaatta agccctttga ggtaaggacc ttccgcataa gataaaaaag agcctccgaa
     2941 attcggaggc tttttatttg ggaaaaagtt gaataatatt cattggtgtc cacggaaata
     3001 ttcactcgct ttgaaaaagc ccgtctttat gcacaaacgg cggcagcgaa attacagcac
     3061 cgtgaacaaa cggcgggcaa atatttttct atatcagaat aataatgccg ttactcgagc
     3121 aacatataca aaaattttta tacattttta taaggttcat acaaattacg aattcccaaa
     3181 ataagctaaa gctgatatac tataagtgca atgagaggtt aaattaaggc ccgtaagtta
     3241 agaaaggatg aatgttatga gcaaaagcct tatttggtcg gttctttcac ttgtagtagc
     3301 agcaagcctg atttacggtt tcgtagttac ggttttctca gaaggcggtc cctttggtcc
     3361 cgaaaagccc tcttcagcag ttgaggcaga ggcagaggcc aacgacgccg gcaatttacc
     3421 cgatgtcatt ttcagctaaa ggtgcatgat gacaatgggt tgtacggctg caagacgcag
     3481 caaaaataaa agaataaata ttaaaactta tgggttaatc tccgtgttaa aataaacgac
     3541 agcaggcgct ttaaagtacc tactgtcgtt gttttatttt attccttttt ctcgccttcg
     3601 ggttcgggtt tagtaaggac attaataatt tcctcaaggg cttcaagggg gccctgattt
     3661 tttagtcttc ttacggagat atatggcttt ttggcggcgg atacgaatat tctgccttta
     3721 agaccgttgg cgagcttaga caccatggtc atatccgttt cgggagtgaa taataacaga
     3781 ctgtcgccac gctgaccaac ctcgtaaata ccgagtcttg cggcggcgtt tctgagcatg
     3841 gagattttga taagccccat aaccgcgtcg ggcgggtcgc cgaatctgtc ggtcagctcg
     3901 tcaagcacat cgtcggcgtc atcctgattc ttaatctccg caattcgcct gtaaatgctg
     3961 aggcgctggg gtacgctttc gatatacttt tcggggatat gggcgtcaac ctgcaggtcg
     4021 ataaggcatt cctcggtttt gacttcggtt tcctcgccct tctctttgct gacttcctga
     4081 gcgaggagtt taaggtacaa gtcatagccc acagcttcca tgtgtccgtg ctggctggcg
     4141 ccgagaaggt cgcccgctcc cctgatttca aggtctcgca tggctatttt aaagcctgag
     4201 ccgaactcgg tgtactccct aatggcgctg agcctgcggt atgcggtttc cgaaagctcc
     4261 ttgcccttga caaatgtgaa atacgcgctg gcgcgtctgg aggacctgcc taccctgccc
     4321 ctcatttgat ggagctgggc aagacccata tggtcggcgt tttcgataat aagtgtattt
     4381 acattgggca catcgacgcc cgtttctata atcgtggtgc agacaagtat atcaatctcg
     4441 ccctcgatga gcctgcgcca aatatcgctc agaacgctct cggacatctt gccgtgggcg
     4501 atttctattc tcgcgtcggg cagatactcc ttaattctga gggcggttcg ctcaattgtt
     4561 tcaatacggt tatggacata atagacctga ccgccgcgcc tgagttcctt agccatagcc
     4621 tcggcgagta tgcccatatc gtgctccaga acataggtct ggacgggctg tctgtcctgg
     4681 ggagcctcct ctattatgga catatccctt atgcccgaca tagccatgtt aagtgtacgg
     4741 ggaatgggtg ttgccgaaat tgtaaggcag tctacggcgg ggaaacgctc tttgagcttt
     4801 tccttttgct caacgccgaa gcgctgctcc tcatcgatta ttaaaagccc caaatcttta
     4861 aactctacgc tgtttgcgat tatgctgtgg gtgcccacaa caatgtctat gctgcctctt
     4921 ttgaggcctt tttttattgc ctcctgctcc ttgggggttc tgaagcggga aagcatttcg
     4981 gcggttatcg gaaagccctc aaaccgtttg agaattgtct ggtagtgctg aagggcgaga
     5041 atagtggtgg gtaccagaat agcgcactgc ttgccgtcgg caacgcattt aaaggcggct
     5101 ctgagagcaa cctcggtttt gccgaatccc acatcgccgc agagcaatct gtccatggga
     5161 tagggctgct ccatgtcgtt cttgatttcg ttgacgcacc ttagctggtc ctcggtttcc
     5221 tcaaactcaa agcgccgctc aaagtcgttc tgcatatcaa tgtcctttga gaacgcaaag
     5281 ccttttgtgg agaccctttt ggcgtagaga gcgagaagct gctttgccat gtccttgaca
     5341 ctcttgcgca ctctgttttt ggtcttttcc cattcggcgc tgccaaggcg gctgagcttt
     5401 acctgccttg cgccctcata gggtcctata tactttgata caaggtcaag ctgagtaacg
     5461 gggacatata aaacatcgtt cttgtcgtac ttgattttta tatagtcctt gaccactccg
     5521 cccgtctcaa ttttttggat tccgtcaaag atgccgatgc cgtggacgga gtgaaccaca
     5581 taatctccct tgacaagctc ctcaagagaa ttaaagccct ctgagatttt aaaacctgta
     5641 ttcttgcccc tgcgcttttc ctccgctcgc ttgccgaagg atatgataag gaccttt
//
LOCUS       DAONVQ010000121         5611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_10635_length_5611_cov_3.859971, whole genome shotgun sequence.
ACCESSION   DAONVQ010000121 DAONVQ010000000
VERSION     DAONVQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10635_length_5611_cov_3.859971"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLD98_07565"
     CDS             <1..255
                     /locus_tag="PLD98_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HQA06135.1"
                     /translation="KEELIAWQKVSGLDIRKLFNTSGNLYREMGLKDKLDGMSFDEKI
                     ELLSTDGLLVKRPILLLGEKVLVGFNEKVWSEALNDGTSL"
     gene            236..721
                     /locus_tag="PLD98_07570"
     CDS             236..721
                     /locus_tag="PLD98_07570"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HQA06136.1"
                     /translation="MTVHPFEPFYNEDSEILILGSFPSVRSREAGFYYAHKNNRFWSV
                     LAYVYGSDTPHTVDEKKAFLKKHRIALWDVIESCDIEKSSDSSVRNVKVNDVKGLLEK
                     TDIKRVFTNGALASRLYNTHIYPITGVRAVNLPSTSSANASWSFERLKEKWAEEINRA
                     Y"
     gene            complement(822..1148)
                     /locus_tag="PLD98_07575"
     CDS             complement(822..1148)
                     /locus_tag="PLD98_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HQA06137.1"
                     /translation="MKLILAIVNNDDSAAVAATLTKEGFSITKLSTTGGFLQTGNTTL
                     LIGAEDDQVDKIKQIIREHSFTRTKTVPTVSSLGRGLGVADTTVKTPVRGATVFVLSV
                     DDFEKL"
     gene            complement(1318..1806)
                     /locus_tag="PLD98_07580"
     CDS             complement(1318..1806)
                     /locus_tag="PLD98_07580"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HQA06138.1"
                     /translation="MERIYIEYDNARITRGEGILLNVELYDGRKFENLEARRLFPITG
                     LDKYITLLDEEGVEKAIIRDVNTLMEESKKNVLDALREYYLIPKITKIYEAYEKYGLL
                     KMRVETDRGEFSFDVKSRNNDIKHLYDGRVLIRDSSDNRYEIRDFRKLDKKSQQVFNP
                     YM"
     gene            complement(1820..2758)
                     /locus_tag="PLD98_07585"
     CDS             complement(1820..2758)
                     /locus_tag="PLD98_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA06139.1"
                     /translation="MLVSFISYASMFYGPVNFFASLNDGYQSALTSAERILDIIDAEP
                     EQNFGKGNTVESLKGKIEYRNVNFSFDKIKKTLTNINLTIEPGDIVGIVGTTGSGKST
                     LVNLLMRFYDNYEGEILVDGINIKDIDIQSFRNQIGYVQQEALMFRDTIFNNIAYSNP
                     SATVEEVIHAADVANAHSFIAKLPDGYDTMLGERGTGLSGGERQRLSIARAVLKNPSI
                     LIFDEATAAVDSETEELIQTAIERLIRGRTTLMIAHRLSTLRKANKIIVVDKGEIIEC
                     GSHEELMALKGKYYKLIEIQSMSEKIRKNKEAENFE"
     gene            complement(2786..4072)
                     /locus_tag="PLD98_07590"
     CDS             complement(2786..4072)
                     /locus_tag="PLD98_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQA06140.1"
                     /translation="MLPDNMLSNQPFPSEFKEKASELISPEEKVLFCLVGDLDLNGYY
                     NDSAVITTDKRILAFDRYHENGVLIVNLADVTDSKVKRMYGNAVLRVYCGEKKIDLFR
                     FTYSIASLADGVAMFYQKIAEGGDPETEKVMVEATFEKMRSFCPKCGRALSSPGAECI
                     SCASKGKLVKKLAGYILPEKYTLMFCLFLSFITTTMALAPPYITRTLVDDVIVSKDLN
                     RLFQTVVTLLLIYIVQYTIGAVRGHYMRKAGSRTVTRLRNDIYAKAQYLPMTFYDKIS
                     TGSVFSRISSDTNNLQSFMLRITQEAVVQAFLALGIIVIMLSLNWKLTLLSLIPVPIV
                     VLGARYFGKKISPIYRRIWRRWSSVSAILSDTLPGIRVIKSFTSEQRAINKFNAYNDE
                     WLKEDMRASVLANIFPNAVTFFVTCGSLLIWGIGGK"
     gene            complement(4286..5212)
                     /gene="prmA"
                     /locus_tag="PLD98_07595"
     CDS             complement(4286..5212)
                     /gene="prmA"
                     /locus_tag="PLD98_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQA06141.1"
                     /translation="MNWTEINIEVPSNFTDTASDIANMTVPYGIYVEDYTDLEEGAME
                     IAHIDLIDESLLEKDKSKAIIHVYIPEEENPEPHIAFLTERLNEAKIPFEIKLGVSDS
                     DEWENNWRKYFKPIEVGERLLIRPIWEDAPDTDRKIINLEPGVAFGTGTHETTRLCLE
                     ALDENMKGREGAAVLDVGCGSGILAVASMLLGAKNALGVDIDPLAVKIAKENGKLNGF
                     TEPELSFICGSLTSQVSGKYDIVLANIVADAIIELSKDVEKFLNDGAVYIVSGIIDIR
                     EDEVKKALSDFGFTVTKRRAENGWVCLECKRD"
     gene            complement(5275..>5611)
                     /locus_tag="PLD98_07600"
     CDS             complement(5275..>5611)
                     /locus_tag="PLD98_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HQA06142.1"
                     /translation="FNLGYGFGDNSAATENIVYYDGVGHKLDDVEFIIPPESYMLPWK
                     FTSSDGRFEGDFYPIIDRQAKIDLLKVVVSDQHQVFGRMTGRAILDDGRTVEFKDFLC
                     SCEDIYNQY"
BASE COUNT         1383 a         1263 c         1359 g         1606 t
ORIGIN      
        1 aaagaggagc ttatagcatg gcaaaaggtt agcgggcttg atataagaaa actctttaat
       61 acaagcggca atctttatag ggagatgggt ttgaaggaca agcttgacgg catgagcttt
      121 gacgagaaaa tagagctttt atctaccgac ggattgcttg ttaagcgacc gatacttctg
      181 ttgggcgaaa aagttttggt gggctttaat gaaaaagtat ggagtgaggc tttaaatgac
      241 ggtacatccc tttgagcctt tttacaatga ggactctgag attcttatct taggcagctt
      301 tccttccgtg cgctcaaggg aggcaggctt ctactacgcg cacaaaaata acaggttctg
      361 gagcgttttg gcctatgttt acggctctga tactcctcat actgtcgatg agaaaaaggc
      421 gtttcttaaa aagcatagaa ttgccctttg ggatgtaatt gagtcatgcg atattgaaaa
      481 aagctctgac agctccgtca gaaatgttaa ggtaaacgat gtgaaggggc ttttggagaa
      541 aacggacata aaaagagtct ttacaaacgg cgcgctggct tcaaggcttt acaatactca
      601 tatttacccc ataacggggg ttagggctgt aaatctcccg tccaccagct ccgcaaacgc
      661 gtcatggagc tttgaaagac tgaaagaaaa atgggctgag gagataaatc gggcgtactg
      721 aaaatacaaa cacaaagcgg gggcaaaaag cccccgcttt attatgaatc tatgaaaaag
      781 gatgcttgaa acacaaagaa agcaaatctc aaaaaagcat attaaagctt ctcaaaatcg
      841 tcaacggaga ggacgaaaac cgtggctccc ctgacgggtg ttttaaccgt tgtgtccgca
      901 acgccgaggc ctctgcccag ggacgataca gtgggaacgg tctttgtgcg ggtaaagctg
      961 tgctccctta taatctgctt tattttgtca acctggtcgt cctcagcgcc tataaggagg
     1021 gttgtgttgc ccgtttgaag aaagcctccc gttgtagaaa gctttgttat ggaaaaaccc
     1081 tcttttgtaa gggtggctgc tactgctgcg ctgtcatcgt tatttactat tgccaagatt
     1141 aacttcattg cgaagaaccc cctaaggttt taaatataaa aagaagagac gcagctttac
     1201 tctctgcgca tttttggtaa ctataaaatg tatcttctgc taagattata cactttatta
     1261 caaaaataat caactgtttt tacgctatgg cactgccagt cacaaaatgg cacaaattta
     1321 catatacggg ttgaatacct gctggctctt cttgtccagc tttctgaaat cccttatctc
     1381 ataacggttg tcgctgctgt ctcttatgag cactctgccg tcgtagagat gtttgatgtc
     1441 gttgtttctg ctctttacat caaaggagaa ctcgcctctg tcggtttcca cacgcatctt
     1501 taaaagaccg tatttttcat acgcctcgta aatctttgtg attttgggta tgagatagta
     1561 ttccctgagg gcgtccagga cattcttctt gctttcctcc atcagggtgt ttacatctct
     1621 gataatcgcc ttttccacgc cctcctcgtc aaggagggtt atgtatttat caagacccgt
     1681 gatggggaaa agccttctcg cctcaaggtt ttcaaacttt ctgccgtcgt agagctcgac
     1741 attgaggagg attccctcgc ctcttgttat tctggcgtta tcgtattcta tatatatgcg
     1801 ttccataaga aaaccatcct tattcaaaat tttcggcttc tttgttcttc ctgattttct
     1861 cggacatgct ttggatttcg atgagcttat agtatttgcc cttgagagcc ataagctcct
     1921 cgtgggaacc gcattctata atttcgccct tgtcgacaac tataatcttg tttgcctttc
     1981 tgagtgtgga gaggcggtga gcaatcataa gagttgttct gccgcggata agcctttcga
     2041 tagctgtctg gataagctcc tcggtttcgg agtcaacggc cgcggtagcc tcgtcaaaga
     2101 taaggattga cgggtttttg agaaccgcgc gggcaattga aagacgctgt ctttcgccgc
     2161 cggagagacc tgtgcctctt tcgcccagca tggtgtcata tccgtcgggc agctttgcta
     2221 tgaaggaatg ggcgtttgca acatccgccg cgtggatgac ttcctcaacg gtagcggagg
     2281 ggttgctgta ggctatgttg ttgaatatgg tatccctgaa cattaaagct tcctgctgaa
     2341 catagcctat ctggttgcgg aaggactgga tgtcaatatc ctttatgttt atgccgtcta
     2401 caaggatttc gccctcgtag ttgtcataga agcgcataag aaggtttaca agggtactct
     2461 tgcccgagcc ggtggtgccg actattccca cgatgtcgcc cggctctatg gtaaggttta
     2521 tatttgtaag ggtctttttg attttgtcaa aagagaagtt gacattcctg tattcgattt
     2581 tgcccttaag gctttcgacg gtgttgccct tgccgaagtt ctgctcgggc tcggcgtcga
     2641 tgatgtcaag gattctctcc gcggaggtaa gggcgctctg atagccgtcg ttaaggcttg
     2701 cgaagaagtt tacggggccg tagaacatac ttgcgtagga gatgaaggat acaagcatac
     2761 cgagggtaat ttcctcgtca atagctcatt tgccgccgat gccccagata agcagggagc
     2821 cgcaggttac aaagaaggta acggcgttgg ggaagatatt ggctagaaca gaagccctca
     2881 tatcttcttt cagccattcg tcgttatagg cgttgaactt gtttattgcc ctttgttcgc
     2941 ttgtgaagga ctttataact ctgatgccgg ggagggtatc ggagagtatc gcgctgaccg
     3001 atgaccagcg gcgccagatg cgcctgtaga tgggcgatat ttttttgccg aaataccttg
     3061 cgcccagaac cacgatggga acggggataa gggaaaggag ggtcagcttc cagttaaggg
     3121 aaagcattat cacgataatg cccagagcaa ggaaggcctg aacaacagcc tcctgagtga
     3181 ttctcagcat gaatgactgg aggttgttcg tgtcgctgct gattcttgaa aagacagagc
     3241 ctgtgcttat cttgtcatag aaggtcatgg gcagatactg agccttggcg tatatgtcgt
     3301 tgcgcagcct tgtaaccgtt cttgagccag ccttgcgcat atagtggccc cttaccgctc
     3361 ctatggtgta ctgtactatg tagataagaa ggagggtaac aacggtctgg aagagtctgt
     3421 ttaagtcttt tgaaactata acatcgtcaa caagtgtcct tgtgatatag ggcggggcaa
     3481 gggccattgt ggttgtgata aaggagagga aaaggcagaa cataagggtg tacttttcgg
     3541 gaagtatgta gcccgccagc tttttgacca gctttccctt tgaggcgcag cttatgcact
     3601 cggcgccggg gcttgaaaga gccctgccgc atttggggca gaatgagcgc atcttctcaa
     3661 aggtggcttc taccattacc ttttcggtct ccgggtcgcc gccctctgca atcttttgat
     3721 agaacattgc cacgccgtct gccagggagg caatggaata tgtaaagcgg aaaaggtcga
     3781 ttttcttctc tccgcagtaa actctgagca cagcgttgcc gtacattctc ttgacctttg
     3841 agtcggtgac atccgcgaga ttcacaataa ggacgccgtt ttcatgatag cggtcaaagg
     3901 caagaattct cttatccgtt gtaatgacgg cggaatcatt atagtagccg tttaaatcca
     3961 aatcgcctac taagcaaaat aaaacctttt cttcggggct tataagctca gaagcttttt
     4021 ccttaaactc cgaaggaaaa ggttggttgg atagcatatt atcgggaagc atacaatctc
     4081 ctccttgaca ggcaagcgaa caaaaatgtc ctttgtcacg caagttatat catatatgag
     4141 gcaaaatcgc aagtttttta cagggatttt ttaacttcta catatcctgc aaataaatct
     4201 aaaatttaat gctattttgt tcaaatcgac gaaagaaaaa tggggatatt tacccccgga
     4261 cacaaaatgt gaagattgct cccatttagt cccttttaca ttcaaggcat acccagccgt
     4321 tttcggctct gcgttttgtg acggtaaagc cgaaatcgga aagcgccttt ttgacctcgt
     4381 cctctcttat gtctatgatt cccgatacga tatatactgc gccgtcgttt aagaattttt
     4441 ctacatcctt cgacagctct attatcgcgt cggcgacaat atttgcaagg acaatatcgt
     4501 atttaccgct tacctgtgaa gtgaggctgc cgcaaatgaa gctgagttcg ggctcagtaa
     4561 agccgttgag ttttccgttc tccttagcga ttttaacagc gagggggtcg atgtcaacgc
     4621 ccagggcgtt ttttgctccc agcagcatgg aggcaaccgc aagtatgccg ctgccgcagc
     4681 caacatcaag caccgccgct ccttctctgc ccttcatgtt ttcgtcaagg gcctcaaggc
     4741 aaaggcgggt tgtttcgtga gttccggtgc cgaatgctac gccgggctca aggtttatga
     4801 tttttctgtc ggtgtcgggc gcgtcttccc atatgggcct gataagcagc ctctcgccga
     4861 cttcaatggg cttaaagtac tttcgccaat tgttctccca ctcgtcgctg tcggaaacgc
     4921 cgagtttaat ctcaaagggg attttagcct cattcagcct ttcggttaaa aaggcgatat
     4981 ggggctcggg gttttcctcc tctgggatat aaacatggat tatagcctta gacttgtcct
     5041 tttccaaaag gctctcgtca ataaggtcaa tatgggctat ctccatggct ccctcttcaa
     5101 ggtcggtgta gtcctccaca tagattccgt agggaacggt catgttggct atgtccgacg
     5161 ctgtgtcggt aaaattagat ggtacctcaa tgtttatctc tgtccagttc atatgcttcc
     5221 tctgtttaaa tttgccttga aggctcgcct ctgcttgcct tcaaggcact tttattaata
     5281 ctgattatat atatcctcgc aggagcaaag gaaatccttg aattcaaccg ttctgccgtc
     5341 gtcaagaatc gctctgcctg tcatcctgcc aaatacctga tgctggtctg acacaacaac
     5401 tttaagtaaa tctattttag cctgcctgtc gattatggga tagaagtcgc cctcgaatct
     5461 tccgtcgctt gaggtgaact tccacggcag catgtagctt tcgggcggaa ttataaactc
     5521 tacatcgtcc agcttgtgac ccacgccgtc atagtaaact atgttctcgg tggcggctga
     5581 gttgtcgccg aagccgtagc caaggttaaa g
//
LOCUS       DAONVQ010000122         5567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_10732_length_5567_cov_5.389332, whole genome shotgun sequence.
ACCESSION   DAONVQ010000122 DAONVQ010000000
VERSION     DAONVQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10732_length_5567_cov_5.389332"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..1506
                     /locus_tag="PLD98_07605"
     CDS             262..1506
                     /locus_tag="PLD98_07605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA06143.1"
                     /translation="MSPSNPYKRTKTACYLAYVSQAAVITLMPLLFTTLREQLGVSYT
                     VLALLTGFNFVTQLSADFFFSSAAAKFGYKPFVLLSMACECAGFTLLALAGSKFSSPV
                     VWLFIATFLFALGGGLSEILISPIINGVPSDSSAGSMAILHSMYSWGQMSVVLITTLL
                     LYILGAKNWPVIVMLWALLPIVCAILFLGAKYPENAVNRSDSTKLQIMKSPIYALCAI
                     TIFFGAGAEHTIVGWSSAFMEEVLSIPKVYGDIAGILMFAFGMAIGRFTHGLLGNRVS
                     LSKSMLIGSMGVFLCMLTMGLSPYPVLSFIACALSGICVALLWPGTLVLASESFPKSG
                     AWLFAFMAVSGDLGAAACPFVTGKLADMAGEISFLADIANRFSLSSEQTGMRTGFVFS
                     SIYGLVCAAALIMFRAKTKKKI"
     gene            complement(1544..2323)
                     /gene="radC"
                     /locus_tag="PLD98_07610"
     CDS             complement(1544..2323)
                     /gene="radC"
                     /locus_tag="PLD98_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQA06144.1"
                     /translation="MGASEKDFLKHSQKEPSKAQTAEDKVTHEGHRKRLKNRFLTSGL
                     DSFEPHNILELLLFYSIPRKDTNETAHLLLKRFGSLSAVFDAPFSELIKVKGISESSA
                     TLIKLIPALSRAYLSDKAKHTKEIDSPEKAASVLIPRFVGETSEVLLVLALDNKGMLK
                     KIIEISRGSVSCTDINMRLMVQELLASNASAAIIAHNHPNGFAVPSKQDFEATRQISK
                     TLQAMDINLLDHIIISGEEFFSMSQNLKYASAFTNGDIAYG"
     gene            complement(2431..3639)
                     /locus_tag="PLD98_07615"
     CDS             complement(2431..3639)
                     /locus_tag="PLD98_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQA06145.1"
                     /translation="MNPIYKAYCRAYQTVFRGAAYLIPWREPELITGAGTVKTLPSII
                     KAKGINNVLIVTDKGITKLGLYNSLVENLEKTGIKYSIYDGTQPNPTINNVNEAKAMY
                     INNRCEAIIAFGGGSPMDCAKLCGAAVVRPKKPIQKMRGLFKVMRKLPPLFAIPTTAG
                     TGSETTIVAVVTDSETHEKYPVNDPQLRPRYAVLDPELTVSLPPSITAATGMDVLTHA
                     VEAYIGRSNTKSTKEAAEKAAKLVFENLYEVYSNGQNLEARENMLVASYFAGIAFTRA
                     YVGYVHAIAHNIGGLYNVPHGLANAIIMPYVLEAYGKYAYKSLAKLADLTGAAGDAAT
                     EEEKAKNFIEAIKEMNRKMEIPDKIAELKEEDIDTIAKRALKEANPLYPVPKLMDRKE
                     IAEVIRRLLP"
     gene            complement(3760..5214)
                     /locus_tag="PLD98_07620"
     CDS             complement(3760..5214)
                     /locus_tag="PLD98_07620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQA06146.1"
                     /translation="MNKKTGFKLLSLLLVLSLLFSMPFAASANTGDEIVPDGSITRVN
                     VTIYGDSKTSRGFSWATKKNSGSDVMLYAIDAEGNPISEVKYYSGKSNSFMGYIYHKV
                     VAADLEPGTMYAYRVGDKAAGKWSDLGTFKTDDGDDKFSFIAIADVQASSEKNFQFAS
                     KVLEAGYRTLPEAEFFMSAGDFVNDCTNEEWEWLFKSFKNIFMNTTAVPIAGNHDGNL
                     KWHWFENQFNLKKGAGCDGITGVYYSFDYGNAHITVLNSNDMYPMSEQQVNWLQNDIN
                     KSDAKWKILVMHRPFYSAGKNVNKPDTMIMRDRLLPIVEKLNIDLIIAGHDHMYYRSK
                     SVKNGKVVPTETITEVYKGVETVFEVNPAGVTHILPNTAGTKRYSVNQKAVSPILDIA
                     AVASQPGLSMFSTFEIDGDRLVYKAYTVDVDNGGTVELYDQYAILKTVPGSAVEGWED
                     LDTNPSNNLFKNIANFFKHFTQVIFSYLKMLASR"
BASE COUNT         1240 a         1377 c         1417 g         1533 t
ORIGIN      
        1 cgagtgacga ctgcccatcg ccctgtcctc tgtaagacca cccctccgcc gctggagtca
       61 ccgccgcccc gcaacacaga gggagaacag ccctgccagc cgaccacccc ccccaacggg
      121 ggttttttgc gctgtcgcaa atgcgcttgc cgcccataga ctgcatacga agggaatttt
      181 gtgggcatta aaaaaaccta ttgacttttt gtcgacggaa tatgatactt tatcattgat
      241 atacaaaaaa aggagaaatt tatgtcccct tccaatcctt ataaaagaac aaaaacagcc
      301 tgctatttgg cttatgtctc ccaggcggcg gtcatcacct taatgcccct tttgtttacc
      361 acgctcagag agcagctcgg cgtcagctac acggtgctcg ccctgcttac gggctttaat
      421 tttgttacac agctttcggc ggacttcttt ttcagctccg cggctgcaaa gttcggctac
      481 aagcccttcg tgctcctctc tatggcttgc gagtgcgcgg gctttaccct gctcgccctg
      541 gcgggctcaa aattctcctc cccggtggtg tggctcttta tcgccacctt cctctttgcc
      601 ctcggcggag gtctgtccga aattctcata agtcccataa tcaacggagt gcccagcgac
      661 tcctcggcgg gcagcatggc tatactccac tccatgtact catggggtca aatgtctgtg
      721 gtgctcatca caacgcttct tttatacata ttgggagcga aaaactggcc cgtcatcgta
      781 atgctctggg cgcttctgcc catagtctgc gctatcttgt tcctcggcgc caaatacccc
      841 gaaaacgccg tcaaccgcag cgactccaca aagctgcaga ttatgaaaag cccgatttac
      901 gccctctgcg ccataaccat tttcttcgga gcgggagcgg aacacacaat agtcggctgg
      961 agctccgcct ttatggagga ggttctcagc atccccaagg tttacggaga tattgcgggc
     1021 attctcatgt tcgctttcgg catggcgata ggcagattca cccacggtct tttgggcaac
     1081 agagtaagtt tgtcaaagtc gatgctaata ggctccatgg gagttttcct ctgcatgctc
     1141 accatgggac tttcccccta ccccgttttg tcctttatag cctgcgccct ttcgggcatt
     1201 tgcgtggcgc tcctctggcc gggcactctg gtgctcgcct cagagtcttt ccccaaatcg
     1261 ggagcgtggc tctttgcgtt tatggcggtc tctggcgacc tcggcgccgc tgcatgcccc
     1321 tttgttacgg gcaagctggc cgacatggcg ggggagattt ccttccttgc ggacatcgcg
     1381 aaccgctttt ctctcagctc cgagcagacg ggtatgagaa cgggatttgt gttctcatcc
     1441 atctacggac ttgtctgcgc cgcagcactc ataatgttca gagccaaaac caagaaaaag
     1501 atataaaaaa acccgcccct gtgagggcgg ttttattttc tgtttatccg taggctatgt
     1561 ccccgtttgt aaaggccgag gcgtatttca gattctggga catggagaaa aactcctcgc
     1621 ccgatataat tatatggtca agaaggttaa tatccatggc ttgcagagtc tttgagattt
     1681 gccttgtagc ctcaaaatcc tgctttgagg ggacggcaaa gccgttgggg tggttgtggg
     1741 cgattatagc cgcgctggcg ttgctggcta gcagctcctg caccataagc ctcatgttta
     1801 tgtccgtgca gctcacgctg cctcggctga tttcgattat tttcttcagc atccccttgt
     1861 tgtcaagggc gagaacaagc aaaacctcgc tcgtttcccc cacaaatcgg ggaatcagca
     1921 cagacgccgc cttttcgggg gagtcgattt ccttagtgtg ctttgctttg tcggagagat
     1981 acgcccttga gagagcggga atgagtttta taagagtcgc cgatgattcg ctgatgccct
     2041 tgaccttaat tagttctgag aacggcgcgt caaagaccgc ggacaaagag ccgaatctct
     2101 taagcaaaag atgagcggtt tcgttcgtgt cctttctggg tatggagtaa aaaagcagca
     2161 gctccagtat gttatggggc tcaaagctgt caagcccgct ggtcaaaaag cggtttttaa
     2221 gccttttcct gtgcccctcg tgagttacct tatcttcggc tgtctgtgcc ttagacggtt
     2281 ccttttgaga gtgctttaag aaatcctttt cagacgcgcc cattttaatg cccctttaag
     2341 tgttgcttta ctgattatac cttaatttca attattttgc aaagaaaaag gcaaaaaacc
     2401 gccccgcttt ccgcgaggcg gtttttctct ttacggaagg agccttctta ttacctcggc
     2461 tatttccttt ctgtccatca gctttggtac tgggtagagg ggatttgctt cctttaaggc
     2521 tctctttgct atggtatcta tatcctcctc tttgagctcg gcaatcttgt cgggaatttc
     2581 catctttctg ttcatttcct tgattgcctc aataaagttc tttgcctttt cctcctcggt
     2641 agcggcgtcg ccggcggcgc ccgtcaggtc agccagcttt gcgaggctct tgtaagcgta
     2701 cttgccgtaa gcctcaagca catagggcat gattatggcg ttggcaagac catgaggaac
     2761 attgtaaagt ccgccgatat tgtgagcgat ggcgtggaca tagcccacat aagcccttgt
     2821 aaaggcaatt cccgcaaagt atgaggcgac aagcatattc tcccttgcct caaggttctg
     2881 accgttgctg taaacctcat agaggttctc aaataccagc ttcgctgcct tttcggcggc
     2941 ttcctttgtg ctcttggtgt tgctcctgcc tatatatgcc tcaacggcgt gggtcaggac
     3001 atccatgccc gtagcggctg tgattgaggg aggaaggctg acggtgagct cagggtcaag
     3061 aacggcgtaa cggggtctga gctgcgggtc gtttacggga tatttctcgt gagtctcgga
     3121 gtctgtgaca accgcgacta tggttgtctc ggagccggtg ccggccgtgg tgggtattgc
     3181 gaacagcggc gggagcttgc gcatgacctt aaacagaccc ctcatctttt ggattggctt
     3241 tttgggtcta acaacagccg cgccgcagag tttggcgcag tccatgggcg agccgccgcc
     3301 aaaggcgatt attgcctcgc agcggttatt gatatacata gccttggctt cgtttacatt
     3361 gttaatggtg gggttgggct gagtgccgtc gtagatggaa tatttaatgc ccgtcttttc
     3421 aaggttttcc acaagggaat tgtaaagtcc cagcttggta atgcccttgt cggtcacaat
     3481 cagaacattg tttattccct tagccttaat aatggagggc agggtcttta cggtgcccgc
     3541 gcccgtaatc agctcaggct ctctccacgg aatgaggtaa gccgccccgc ggaaaactgt
     3601 ctgataagct ctgcagtatg ccttatagat agggttcatg gggaccttcc tttcgtttaa
     3661 tttacaaatt ctgttaaatc cattatataa tcttttcgga ttttgtccag attatataaa
     3721 aaaaataagc ccggagcttt tgctccgggc gctgctgttt tatctggatg ccaacatctt
     3781 gaggtaggag aaaataacct gagtgaagtg cttgaagaag ttggcgatat tcttaaagag
     3841 gttgtttgaa ggattggtgt caaggtcctc ccagccttca actgcggagc cgggaacggt
     3901 cttgaggatg gcgtactggt cgtaaagctc tacggtgccg ccgttatcaa catcaacggt
     3961 gtatgccttg taaacaagac ggtcgccgtc aatctcaaag gtggagaaca ttgaaagtcc
     4021 gggctgggaa gcgacagcgg cgatatcaag aatcggggat acggctttct ggtttacgct
     4081 gtatctcttt gtgcctgctg tgttgggcag gatgtgggtt acgcccgcgg ggttgacctc
     4141 aaatacggtt tcaacgccct tgtaaacctc tgtgattgtc tctgtgggaa caaccttgcc
     4201 gttctttacg gactttgacc tgtagtacat atggtcgtga cctgcgataa taaggtctat
     4261 gttcagcttc tcaacaatgg gaagcagacg gtctctcatt atcatggtgt cgggcttgtt
     4321 gacattcttg cctgcggagt agaagggacg gtgcattaca agaatcttcc actttgcgtc
     4381 ggacttgttg atgtcgttct ggagccagtt gacctgctgc tcggacatcg gatacatgtc
     4441 gttggagttg agaaccgtaa tgtgagcgtt gccgtagtcg aaggaatagt aaacgcctgt
     4501 gatgccgtcg cagcctgcgc ccttcttaag gttgaactga ttctcgaacc agtgccactt
     4561 gaggttgccg tcatggttgc ccgcaatggg aaccgcggtg gtgttcataa agatgttctt
     4621 aaatgacttg aacagccact cccactcttc gttggtgcag tcgtttacaa agtcgcccgc
     4681 gctcatgaag aactcagctt cgggcagtgt cctgtagcct gcctcaagaa ccttgctggc
     4741 aaactggaag tttttctcgc tgcttgcctg aacatcggcg atggcgatga aggagaactt
     4801 atcgtcgccg tcgtctgtct taaaggtgcc gaggtcgctc cacttgccgg cagccttgtc
     4861 gcctactctg taagcgtaca ttgtgccggg ctcaaggtcg gcagcgacaa ccttatggta
     4921 tatgtagccc atgaaggaat tgctcttgcc tgaatagtac ttaacctcag aaatggggtt
     4981 gccttcagcg tcaatggcgt aaagcattac atctgagccc gagtttttct tggttgccca
     5041 tgaaaagccg cggcttgttt tgctgtcgcc gtaaatggtt acattgactc ttgtaattga
     5101 gccgtcggga actatctcat cgcctgtgtt tgccgatgcg gcaaagggca tggagaacag
     5161 aaggctcaaa acaagaagaa ggcttaaaag cttaaagccc gtttttttgt tcatttaaat
     5221 aacctccaga attttgctgt aaataaagat acacttacag tttaagtttt attgggacaa
     5281 atgtcaatat aacaggtgtc acaaaggcac aaaattttaa gacattatga acgatgattt
     5341 gtcaaaatga catttaatcc gattttcaaa gcataagcgg agaaatgcgg caaataatta
     5401 tgttgttaaa tttattaaac gggtgattta atgaactttc ggacagacct agtacttgaa
     5461 agacgggagc ttgtaggcga agcccccgtt ccgggtgtaa atcaggtaac aagagaaaat
     5521 gaaaacgcca aaattacaga aattcaggtt ctgaccgaag aaggggc
//
LOCUS       DAONVQ010000123         5491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_10908_length_5491_cov_5.229029, whole genome shotgun sequence.
ACCESSION   DAONVQ010000123 DAONVQ010000000
VERSION     DAONVQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10908_length_5491_cov_5.229029"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PLD98_07625"
     CDS             complement(<1..1378)
                     /locus_tag="PLD98_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HQA06147.1"
                     /translation="MDEVITSNFIHDIIDEDLKNGVNSRVQTRFPPEPNGYLHIGHAK
                     AIYIDFKTAEKYGGICNLRFDDTNPVKEDVEYVDAIMEDIKWLGFKWANVYYASDYFD
                     FLFECAVKLIKQGDAYVCDLSADEIREYRGTLTEPGKNSPYRDRSVEENLDLFYRMAK
                     GEFPDGSRVLRAKIDMASPNLNMRDPVMYRIAHVPHHQTGDKWCIYPMYDFAHPLSDA
                     KEGVTYSLCSLEFENHRPLYNWFIEKIGFKEPPKQIEFARLNLNYSVMSKRKLLEMVN
                     KKIVSGWDDPRMPTLCGLRRRGYPPEAIIDFIEKVGVSKAYSIVDIGLLESCVRDRLN
                     SNAPRLMAVLDPIKLVIDNYPDDLVEELPVEYHPDHEEMGSRNVTFSKELYIERSDFM
                     IEPVKKYYRLYPGNEVRLKSAYIVKCTSYETDENGNVTCVHCTYDPETRGGNAPDGRK
                     VRGTIHWVN"
     gene            complement(1395..3053)
                     /gene="gltX"
                     /locus_tag="PLD98_07630"
     CDS             complement(1395..3053)
                     /gene="gltX"
                     /locus_tag="PLD98_07630"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQA06148.1"
                     /translation="MSETDFNALAELLYPDIDKTPEDYEKIYPERQLKEGAKVTRFAP
                     SPTGYIHIGNLFTAMISKLAAEVSGGVFFLRIEDTDKKREVENGVSAILSGLSQYGIV
                     PMEGMTGEDSEKGAYSPYKQSLRKEIYACFAKDLVRKGLAYPCFCTEEELNEMREKQV
                     QAGVNTGYYGEWAKCRNLSFEEQKSLIESGRPFVLRLRSQGNPENYIKTEDMLRGRME
                     MPENNMDVVLIKSDGIPPYAFAHAVDDHLMRTNLVVRSDEWIASLPAHLEIFKALGFK
                     PPKYAHISPIMKLDEGNKRKLSKRKDPEAAVSYYSQLGYPKDSVLEYLMTLANSNFED
                     WRRANPDAPLSAFPFNIKKMSQSGALFDLLKLNDVSKNVISKMPAEEVASLVLQWSRE
                     YDPELYSIIKDDEKYLTSIFSIDRGGKKPRKDIAMWSEVKDYISYFYDSLFKPDYNLP
                     ENIRPEDAKAICKAYVKVFDATDDKDTWFNKIKGICPELGFCPDVKAYKADPAGYKGH
                     VGDVASVIRLAVTGRLNTPDLHSILSLLGQERAVNRLNSFSESI"
     gene            complement(3254..5104)
                     /locus_tag="PLD98_07635"
     CDS             complement(3254..5104)
                     /locus_tag="PLD98_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HQA06149.1"
                     /translation="MKNSKRTFGLKALSFILVAALFLSISVPAFAVDNEPSFLYEFID
                     GESISIIEADLDTSIDKTLVIPSEIDGYTVVAIGSRTFADYKDLEAVEIPATVKKIGA
                     EAFAGCTNLNTVSIRGIIEECGMGAFNDTAWYKNFDIDFVIINVNSPQAMLIGYKGND
                     KDVKVPITIAKIGDGAFKGNKTVETVILPERTTVIGASAFENCSALTEVTALGDITVV
                     GENAFKGTKWFEEYPGDYIGIGKVFIKYIGNEEVAVIPNTYTVVSAGAFYGCKNTFAV
                     KVPASVTSIGDSAFFTFSDSMNNHYAEIFAYAGTYAIDYAKANGINHTVLNRPGDVNG
                     DGNATAADARLILRMVAKLDKVTTDKALVADVTGDNAVKADDARMLLRMVAKLDKVTP
                     EDLLSKPSSKVEILNAYSEAVKFALNSGAGYTKKTYQELKNIKLNFFTELNLMFLQNK
                     IVTTKPKNAKNQVYDVDSVSALTNLHLPTLISTKNIKSATCTIDGKGKYVITIVMGKE
                     TARDGFVTDTQKLLPAASRKNFDDLLKDQLWYKSSLTKFNYTLNYNDSKVVAVVDIKT
                     GFLESITMTINHDFEITGQIYLTKITDGSGGNVGKATRVDTYEFTEFKYS"
BASE COUNT         1277 a         1255 c         1353 g         1606 t
ORIGIN      
        1 cgtttaccca gtggattgtg cccctgacct tcctgccgtc gggagcgttg ccgccccttg
       61 tttcggggtc ataggtgcag tgaacgcagg ttacattgcc gttttcgtcg gtctcgtatg
      121 aggtgcactt tacgatatat gcgctcttga gccttacctc gttgccggga tagaggcggt
      181 aatatttctt tacgggctca atcataaagt ctgagcgctc tatataaagc tccttggaga
      241 aggtgacatt gcggctgccc atttcctcat ggtcggggtg gtattcgaca ggcagttctt
      301 ccacaaggtc atcggggtaa ttatcgataa cgagcttaat ggggtcaaga accgccatga
      361 gcctgggagc gttgctgttg agtctgtcac ggacgcagga ctcaagaaga cctatgtcca
      421 cgatgctgta tgcctttgaa acgcccactt tttcgataaa gtcgattatt gcctcgggcg
      481 gatagccgcg ccttctaagg ccgcagagtg tgggcatcct cgggtcgtcc cagccgctga
      541 cgattttctt gttgaccatc tccagaagct ttctcttgct cattacggag tagttgagat
      601 taagacgggc gaactcaatc tgcttcggag gctccttaaa gccaatcttt tcgatgaacc
      661 agttgtaaag gggtctgtgg ttctcgaact caagggagca aagtgagtag gttacgccct
      721 ctttggcgtc ggagagagga tgggcaaagt cgtacatggg atagatgcac cacttgtccc
      781 ctgtctgatg gtggggaaca tgggcgattc tatacataac ggggtccctc atgttaaggt
      841 tgggggacgc catgtcgatc ttagccctta aaacccttga tccgtcgggg aactcgccct
      901 ttgccattct gtagaaaagg tccagattct cctcaacgct cctgtccctg taggggctgt
      961 ttttgcccgg ctctgtgagg gttcctctgt attcccttat ctcatcggcg gacaggtcgc
     1021 atacataagc gtcgccctgc ttgatgagct ttacggcgca ctcaaagagg aaatcgaaat
     1081 agtcggaggc ataatagaca ttggcccatt taaagcctag ccacttaatg tcctccatta
     1141 tggcgtcaac atattcaaca tcctctttta cggggttggt gtcgtcaaat cttaaattgc
     1201 atattccgcc gtacttttcg gcagtcttaa agtcaatata tatagccttt gcgtgaccta
     1261 tatgaagata accgttgggt tcagggggga aacgggtttg aacccttgag ttgactccgt
     1321 ttttaaggtc ctcgtcaata atgtcgtgga taaaatttga agtaattact tcatccatta
     1381 cttttcctcc aattttatat gctttctgag aatgaattaa gtctattaac cgctctttcc
     1441 tgacccaaaa gggagagaat ggagtgcagg tcgggagtgt tgagcctgcc cgttacggca
     1501 aggcgaatga ccgatgccac atcgcccaca tgacccttgt agcctgcggg gtcggccttg
     1561 taagccttta catcggggca gaagccgagc tcggggcaga tgcccttaat cttattgaac
     1621 catgtgtcct tgtcgtcggt cgcatcgaaa accttaacat atgctttgca gatggctttt
     1681 gcgtcctcgg gtctgatatt ttcgggcaga ttgtagtcgg gtttaaagag gctgtcgtag
     1741 aaataggaaa tataatcctt aacctcagac cacatggcga tatccttgcg gggctttttg
     1801 ccgccgcggt cgattgagaa gatggatgtc aggtactttt cgtcatcctt tattatgctg
     1861 taaagctcgg ggtcgtattc cctgctccac tgcagcacca gacttgccac ttcctcggcg
     1921 ggcatttttg agatgacatt cttgctgaca tcgttaagtt tcagaaggtc aaacaatgcg
     1981 ccgctctggc tcattttctt tatattaaag gggaaggcgg aaaggggcgc gtcgggattt
     2041 gcccttctcc aatcctcaaa gttcgagttc gcgagggtca taaggtactc gagaacgctg
     2101 tccttcggat agccgagctg ggagtaatag cttacggcgg cctcagggtc ctttctcttg
     2161 ctgagctttc tcttgttgcc ctcgtccagc ttcataatgg gcgagatgtg agcgtacttg
     2221 gggggcttaa agcccagagc cttgaagatt tcaagatggg cgggtaggga ggctatccac
     2281 tcgtcgctcc tcacaacaag gtttgtccgc attaaatggt cgtccaccgc atgggcaaag
     2341 gcataaggag gaatgccgtc ggatttgatg agaaccacat ccatattgtt ctcgggcatt
     2401 tccattctgc ctctcagcat atcttcagtt ttgatataat tctcgggatt gccttgtgag
     2461 cgaagtctca aaacaaaggg gcgtccgctc tcgataaggc tcttttgctc ctcaaagctg
     2521 aggttgcggc actttgccca ttcgccgtaa tagcctgtgt ttacgcctgc ctgaacctgc
     2581 ttttcgcgca tttcgttaag ctcttcctca gtgcagaagc agggataggc aaggcccttt
     2641 ctgacaaggt ccttcgcgaa acaggcatag atttcttttc tcaggctttg cttgtagggg
     2701 ctgtatgcgc ctttttcgct gtcctccccc gtcatgccct ccatcgggac aatgccgtac
     2761 tgggaaagac ctgaaagaat cgcgctgacc ccgttttcta cctctctttt cttatcggtg
     2821 tcctcaattc tgaggaaaaa aacgccgcct gaaacctccg ccgccagctt gcttatcatg
     2881 gcggtgaaaa ggttgcctat atggatatat cctgtggggc tgggggcaaa gcgggtaacc
     2941 tttgcgccct ctttaagctg cctttcggga taaatctttt cgtaatcctc gggagttttg
     3001 tctatatcag gatacaaaag ctctgctaaa gcgttgaaat cggtttccga cataatatcc
     3061 tccgaatttt gtttgtgtct taccctattg gataattttt cttataaaga ctttcgatat
     3121 tataacataa tcagacacat cttgcaaaca gattagcttt tttataggct ttgcagtatt
     3181 tgaaaatata aacaaatcaa aaggaaaaga aataaaaaaa gcggtgcttt tggcaccgct
     3241 tttattttat tgtttaggag tatttgaact ctgtgaactc gtaggtgtcg actcttgtgg
     3301 ccttgcctac atttccgccg ctgccgtcag ttatcttggt gagataaatc tgaccggtga
     3361 tttcaaagtc gtggttgatg gtcattgtga tggactcaag gaagcctgtc ttaatgtcaa
     3421 caaccgctac gaccttgctg tcattgtagt taagggtgta gttgaacttt gtaaggctgg
     3481 acttgtacca gagctggtcc ttgagcagat cgtcaaagtt ctttcttgaa gcagcgggga
     3541 ggagcttctg ggtatcggtt acaaagccgt ctcttgctgt ctccttgccc attactatgg
     3601 tgattacata cttgcccttg ccgtcaattg tgcaggttgc gctcttaatg ttctttgtgc
     3661 tgatgagcgt gggcagatgc agatttgtaa gagctgatac ggagtcaaca tcataaacct
     3721 ggttctttgc gttcttcggc tttgtggtaa caatcttgtt ctgcaggaac atgaggttga
     3781 gctctgtgaa gaagttgagc ttaatattct ttaactcctg atatgtcttc ttggtgtagc
     3841 ctgcgccgct gttgagagcg aactttacag cctcggaata tgcgttgagg atttcaacct
     3901 tgctggaggg cttgctgagt agatcttcgg gtgtgacctt gtcaagcttt gcaaccattc
     3961 tcagaagcat acgggcgtcg tcagccttaa cagcgttgtc gcctgttaca tccgcgacaa
     4021 gagctttgtc ggttgtgacc ttgtcgagct ttgcgaccat tcttaagata aggcgggcgt
     4081 ctgcagctgt ggcattgccg tcgccgttaa catcgccggg acgattgaga acggtgtggt
     4141 taatgccgtt tgcctttgca taatctattg cgtatgtgcc tgcatatgca aaaatctcgg
     4201 cgtagtggtt gttcatcgaa tcggagaagg tgaagaaagc tgaatcgccg attgatgtaa
     4261 cggaagcggg aaccttaacc gcaaaggtgt tcttgcagcc gtagaaagct cctgcgctga
     4321 ctactgtgta agtattgggg ataacggcaa cttcttcatt gcctatgtat ttgatgaata
     4381 ccttgccgat gccgatatag tcgcccggat actcctcgaa ccactttgtg cccttaaatg
     4441 cgttctcgcc tacaactgtg atgtcgccga gagcggttac ttctgtaagg gctgagcagt
     4501 tttcaaatgc ggaggcgccg ataactgttg ttctctcggg gaggatgacg gtctcaactg
     4561 tcttgttgcc cttgaaagcg ccgtcgccga tcttagctat ggtaatggga accttgacat
     4621 ccttgtcatt gcccttgtat ccgatgagca ttgcctgcgg gctgttgaca tttataatta
     4681 cgaaatcaat atcaaaattc ttataccaag ctgtatcgtt gaatgccccc ataccgcact
     4741 cttcgattat gcctctgatt gatacggtgt taaggtttgt gcagcctgca aaagcctcag
     4801 cgccaatctt cttgaccgta gcgggaatct ccactgcctc aaggtcctta taatccgcaa
     4861 aggttctgct gccgattgcg acaaccgtat atccgtctat ctcagatggg ataaccaatg
     4921 tcttatcaat tgatgtatcc agatctgcct ctataatcga aatgctttct ccgtcgataa
     4981 actcatagag gaaggaaggc tcattatcca cagcaaatgc gggaaccgaa atgctgagga
     5041 acagagcggc aacgagaatg aaggaaagcg ccttcaaccc aaatgttctt tttgaatttt
     5101 tcatcgggga actcctccta acacaatata tggggttaag tgcggcaaat aaatacagct
     5161 gatttactat gttaatgcat agatgtctac gataagaagt ataacctata tagtatgcca
     5221 atgtcaacga attttatgaa aattgtctga tataatcata taatttctgg caatttagga
     5281 attaattaca aaatgtttga ggactgttga taaattgttt ccaaattaaa aactttgcat
     5341 ccacagagga aaaaattgcg ataggttttc aaaatttttc ctcgaaatga cgcctcattc
     5401 gttttgaata aacagacttc ttgcctgggg gattaagtcg tctccccaaa gtcttaagtg
     5461 agaaatgtcg tttgccgcct cgagaaggat t
//
LOCUS       DAONVQ010000124         5394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_11150_length_5394_cov_5.468627, whole genome shotgun sequence.
ACCESSION   DAONVQ010000124 DAONVQ010000000
VERSION     DAONVQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11150_length_5394_cov_5.468627"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLD98_07640"
     CDS             <1..599
                     /locus_tag="PLD98_07640"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA06150.1"
                     /translation="PLSYILSKLKNGRGRFVVFLFVLPMWMNHILRTLALQNLLSNTG
                     VINGILEFLRLPTLNIMNTPTAIIIGMVYTYLPFMILPIYNSIAKIDDGVIDAAYDLG
                     ASGFTVFRRLILPLSLPGIISGITMVFVPSISEFVIADMLGGSKTLLIGNVIEQEFTV
                     GSNWNLGSGLSVVLMVFIVASMVIMQRYDGDGEGAALW"
     gene            593..1435
                     /locus_tag="PLD98_07645"
     CDS             593..1435
                     /locus_tag="PLD98_07645"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQA06151.1"
                     /translation="MVKKLFSFIQKFYVAIIFLILYSPVIVLMVLSFNDSKSRVIWGG
                     FTLKWYSRMFNSSIIVETFIVTVTLALLSAAIAVVLGTAACVGIEAMRRRNKQIVLGA
                     TNIPLLNADIVTGISLMLFFSRFLNAGFPTLLIAHITFELPYVILSIMPRMRQINTNA
                     YNAALDLGATPLYAFFKIILPEIFPGIITGFIMALTMSLDDFTISYFTKGPGVSTLPT
                     MIYSELRKGVKPEMYALSTLMFLAVLILLVIVNLKGSPMLSKPFANAKNGGKKHAQTM
                     RYRR"
     gene            1493..2551
                     /locus_tag="PLD98_07650"
     CDS             1493..2551
                     /locus_tag="PLD98_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQA06152.1"
                     /translation="MKKVISLVLALAMVIGVFALSGCGKETNGSINVLSLGDYIDMNI
                     LKTFEKETGIKVKYDELPTNEDIYTKFQTSSVKYDVLCLSDYMIERLIKENRLLEINF
                     DNLPEAKKNIGETYWKMSEEFDPDRKYNVPHFYGAVGILYNSKKVDEEKVQSWNVLWD
                     EAYSQRIIMQNSMREAFIVPLIILGYDINTTNIDELNEAKEMLIKQKPLVQRYLVDEA
                     RDEVIAENADLAVVYSGEAYIGTQGNPDLRFVIPKEGSSFWFDSWVIPDTCRNKEGAE
                     MFLNFLCRKDIATLNFEAIYYSTPNQAVIDSLDDELRNNPELFLDDEELARCYSLKYL
                     GQEIADYYSQAWKEIKAE"
     gene            2837..3544
                     /locus_tag="PLD98_07655"
     CDS             2837..3544
                     /locus_tag="PLD98_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HQA06153.1"
                     /translation="MSDCKAKPKIKITDGGPYIVTGGVKLSEKIITPVGNGYAWTDGE
                     PLPQSEVYALCRCGKSKNPPFCDGFHVKSGFKGTETAEKNTFMERAKVYEGAELDLYD
                     DGRCAFARFCHRNTGNVWELVRNSNNPFLKEEAIKAASECPAGRLIAADKQGNLIEPE
                     LEPEIIIVQDPDRGCSGGIYVKGDIEIESADGTLYEHRGRSVLCRCGQSDNTPFCDAS
                     HVSYMFRVKKKGLLDLL"
     gene            complement(3617..4201)
                     /locus_tag="PLD98_07660"
     CDS             complement(3617..4201)
                     /locus_tag="PLD98_07660"
                     /inference="COORDINATES: protein motif:HMM:NF025635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HQA06154.1"
                     /translation="MLGGDSNRFDAFSDGIEPGGLRNRDEIKLLICYMLKSLGKEIPK
                     KLICDSLTLESLANYFEINNAISELEKAGHVIKTDEESESYAVSESGETIAQGLDVML
                     PRTVREKAISAVLKLTTLARRELENNIHVEKSGSGYHITFSSVENGEEIIGIRLFASD
                     MMQIERLREGFLLNPSRVYEAVINALSENAKEDI"
     gene            complement(4214..5128)
                     /gene="metA"
                     /locus_tag="PLD98_07665"
     CDS             complement(4214..5128)
                     /gene="metA"
                     /locus_tag="PLD98_07665"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HQA06155.1"
                     /translation="MPIKIPSTLPAKKYLEDENVFVMTEERALTQDIRPLRILILNLM
                     PTKIVTETQLLRLLGNTPLQVEIELMHMESHESKNTSKEHLLKFYKTFREVKDQKFDG
                     LIVTGAPVEKLEFEEVDYWEELCEVFKWCRKNVYSSFYICWGAQAALYYNYGIKKYPL
                     EKKMFGVFPHRNLDPYHPLMRCIDDIFLAPHSRHTEIKMSDIALVKDLQVLSYSDIAG
                     VHIISDMACRHFFVTGHSEYDVDTLKLEYERDLSRGLPIEMPQNYFPDNDPTKKPLMT
                     WRQAGSQIMANWLNYFVYQQTPYDLSTL"
     gene            complement(5148..>5394)
                     /locus_tag="PLD98_07670"
     CDS             complement(5148..>5394)
                     /locus_tag="PLD98_07670"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HQA06156.1"
                     /translation="FDSDSTLYYNPYITEAVEFYKKSEKMLSDLRTSAITMSQTVQPG
                     VRCTQYDTGAVIYVNYNSYPVTVNEITVPAQDCIRVS"
BASE COUNT         1353 a         1364 c         1296 g         1381 t
ORIGIN      
        1 atccgctttc atatatcctt tctaagctca aaaacggcag gggcaggttt gtggtttttc
       61 ttttcgtgct gcccatgtgg atgaaccata ttctccgcac actcgctctg cagaaccttc
      121 tttccaacac gggcgttata aacggcattc tggagtttct gcgccttccc acacttaata
      181 taatgaatac tccgaccgca atcataatag gtatggtcta cacctacctg ccgtttatga
      241 tactgcccat atacaactcc attgcaaaga ttgacgacgg agttatagac gccgcgtacg
      301 atttgggcgc cagcggattt acggtattca gaagactcat tctgcccttg tccctgcccg
      361 gaataatcag cggcataaca atggtattcg taccctccat ttcagaattc gttatcgccg
      421 atatgctggg cggcagcaag actctgctca taggcaatgt aatcgagcaa gagtttaccg
      481 taggcagcaa ctggaatctg ggctcgggac tttccgtagt gctcatggta ttcattgttg
      541 caagcatggt cataatgcaa agatatgacg gcgacggaga gggggctgca ttgtggtgaa
      601 aaagcttttc tcttttattc aaaagttcta tgtagcgata attttcctga ttttgtattc
      661 ccccgttatc gttctcatgg tgctttcctt taatgactct aaatcaaggg tcatttgggg
      721 aggctttacc ctcaaatggt attcgagaat gttcaacagc agcatcatag tggagacatt
      781 tatcgtcacc gttaccctcg cgctgctttc cgccgccatc gcggtcgttc tcggcaccgc
      841 ggcttgcgtc ggcattgagg caatgcgccg cagaaacaag cagatagttc tgggagccac
      901 caacattccc cttttgaacg cggatattgt taccggtatc tcccttatgc tgttcttctc
      961 aagatttctc aacgcgggct tcccgaccct gcttattgcc cacataacct ttgagctgcc
     1021 atatgttatc ttaagcatca tgccccgcat gaggcagatt aacacaaacg cgtacaacgc
     1081 cgcgctggat ttgggcgcga ctcctttata tgccttcttt aaaatcatac tgccggagat
     1141 tttccccggc atcataacgg gctttatcat ggcgctcacc atgtcccttg acgactttac
     1201 aatttcctac tttacaaagg gtccgggcgt ttccacactg cccacaatga tatattcgga
     1261 gcttagaaag ggcgtaaagc ctgagatgta cgcgctctca acgcttatgt tccttgcggt
     1321 tctgattctg cttgttatag tgaacctcaa gggttcgccc atgctctcaa agccctttgc
     1381 caacgccaag aacggaggca aaaaacacgc gcagacaatg cgctacagaa gatgagtcag
     1441 ggtataaggc ttattattct ctattattaa tttacgaatg gagtgtagtg aaatgaagaa
     1501 agttatcagc cttgtacttg ctcttgcaat ggttatcggt gtttttgccc tttccggctg
     1561 cggaaaagaa acaaacggca gcatcaatgt gctcagcctc ggcgactaca ttgacatgaa
     1621 cattctcaag acctttgaga aggaaacagg catcaaggtc aaatacgacg aacttcccac
     1681 caacgaggat atttatacca agttccagac atcgtcggta aaatacgatg tcctctgcct
     1741 ttccgactat atgattgaaa ggcttatcaa agaaaacaga ctgcttgaga ttaactttga
     1801 caacctcccc gaagctaaga agaacatagg cgagacctat tggaagatgt ccgaggagtt
     1861 cgaccccgac agaaagtaca atgtgcccca cttctacgga gccgtcggaa ttctctacaa
     1921 cagcaaaaag gtggacgagg agaaggttca gagctggaat gtgctttggg acgaggctta
     1981 ttcccagaga atcatcatgc agaacagcat gagagaggct ttcatagtgc cccttatcat
     2041 cttgggctat gacatcaaca caaccaatat tgacgagctg aacgaagcca aggaaatgct
     2101 cataaagcaa aaacctcttg ttcagcgtta ccttgttgac gaggcaaggg acgaggtcat
     2161 agccgaaaac gccgaccttg cggttgtata ttcgggcgag gcatatatcg gcactcaggg
     2221 caaccccgac ctcagattcg taatcccgaa agaaggctcc agcttctggt tcgactcatg
     2281 ggtaatcccc gacacctgcc ggaacaagga gggcgcggag atgttcctca acttcctgtg
     2341 cagaaaggat attgccacgc ttaatttcga ggccatctac tattcaaccc ccaatcaggc
     2401 ggttatagac agccttgacg acgagctgag gaacaatccc gagctgttcc ttgacgatga
     2461 agagcttgcc cgctgctata gtcttaaata tctcggacag gaaatcgccg actactacag
     2521 ccaggcatgg aaggaaatca aggcagagta aaacaaaatg aaagcagggc taaacaccct
     2581 gcttttttta tgcccgcccc gccccgctga gccgccccac cccgccgtgg ggagcggctt
     2641 ctgcctgcca aacggtgagg gcagcccgcc ccgtcccgtt gctcgactgc agtggggata
     2701 ggacgccgca cacgccccgc ccccaatgcc ttgcgacggt tttgtgaact ctgcggtaat
     2761 tgaaactttg atttttttct tgcctaaaca agttttaaag agttataatt agcaaaacag
     2821 tttaacggag gactgtatgt ctgactgcaa agcaaagccg aagattaaga ttacggacgg
     2881 cggcccctac atagttacgg gcggggttaa actcagcgaa aaaatcatca cccccgtagg
     2941 caacggctat gcctggaccg acggagagcc gctgccgcaa agcgaggttt acgcgctctg
     3001 ccgctgcgga aagagcaaaa atcccccatt ctgcgacggc tttcatgtga agagcggctt
     3061 taagggcacc gaaaccgcag agaaaaacac ctttatggaa agagccaagg tctatgaggg
     3121 cgccgagctg gacctttacg acgacggtcg ctgcgccttt gcccgcttct gccacagaaa
     3181 tacgggcaat gtctgggagc ttgtgcgcaa ctcaaacaac ccctttttaa aggaggaggc
     3241 aatcaaggcg gcctccgaat gccccgcggg caggctcatc gccgccgaca agcagggcaa
     3301 tctcatagag cccgagctgg agcctgaaat catcattgtt caggaccctg acaggggctg
     3361 cagcggagga atttatgtaa agggcgatat tgaaattgag tccgccgacg gtacgcttta
     3421 cgagcacaga ggcaggagcg ttctttgccg ctgcgggcag tcggacaata cccccttctg
     3481 cgacgcctcc catgtttcct atatgttcag ggttaagaaa aagggcctcc ttgatttgct
     3541 ctaaaaacaa aaagaccgct tatgcggtct tttttattgc cgaaaataaa aggactgccc
     3601 tcgcagtcct ttatatttat atgtcctctt ttgcgttttc ggagagggcg tttataaccg
     3661 cctcatacac ccttgagggg ttgaggagaa aaccttctct caacctttca atctgcatca
     3721 tgtcagaggc aaagagcctg attcctataa tctcctcgcc gttttccacg ctgctgaagg
     3781 tgatgtgata gccgcttccg ctcttttcaa catggatgtt gttttcaagc tccctgcggg
     3841 caagggtggt gagtttcagc accgcggaaa tagccttctc ccttactgtg cgggggagca
     3901 tcacatcaag accctgagct atggtctcgc cgctttcgga tacggcgtag ctctcgctct
     3961 cctcgtcggt cttaattaca tgccccgcct tttcgagctc ggatatggcg ttgtttatct
     4021 caaaataatt cgcaagggat tcaagcgtca agctgtcgca aatcagcttt ttgggtatct
     4081 ccttgccaag gcttttgagc atatagcaaa ttaagagttt aatttcgtca cgatttctca
     4141 gaccgcccgg ctcaatgccg tcggagaaag cgtcaaatct gtttgagtca ccgccaagca
     4201 taaaatcacc ctgttaaaga gttgataaat cgtaaggagt ttgctgatat acaaagtagt
     4261 taagccagtt ggccataatc tggctgcccg cctgacgcca ggtcataagc ggctttttgg
     4321 tcgggtcgtt gtcggggaag tagttttggg gcatctcaat cgggagcccg cggcttaaat
     4381 ccctctcgta ctccagttta agagtatcca catcatactc cgagtgaccc gtcacgaaga
     4441 agtggcggca tgccatgtcg gagattatat gtacccccgc gatgtccgag taggagagca
     4501 cctgcaaatc cttaacaagg gctatgtcgg acatctttat ttccgtatgc ctcgaatggg
     4561 gagcgaggaa aatatcgtca atgcacctca tgagaggatg ataggggtca agattcctgt
     4621 ggggaaagac gccgaacatc ttcttctcaa gaggatactt ctttatgccg tagttgtagt
     4681 aaagagccgc ctgagcgccc cagcagatgt agaaggagga gtaaacattc tttctgcacc
     4741 acttaaagac ctcgcaaagc tcctcccagt agtcaacctc ttcaaactcc agtttttcga
     4801 cgggcgcgcc tgttacaatc agcccgtcaa acttctggtc ctttacttct ctgaaggtct
     4861 tgtagaattt gaggagatgc tccttgctgg tgtttttgct ctcatggctc tccatgtgca
     4921 taagctctat ttcaacctgc agcggagtgt tgccgaggag ccttaaaagc tgagtctcgg
     4981 taacaatctt tgtgggcatg agattcagta taagtattct aagagggcgt atatcctgtg
     5041 tcagcgccct ctcttcagtc ataacgaaaa cattctcgtc ctcaagatat ttttttgcgg
     5101 gaagtgttga cggaatttta atcggcatag caactctcct ttgcttatca gcttaccctg
     5161 atgcagtcct gagcgggaac cgtaatttcg tttacggtaa cagggtagga attgtagttt
     5221 acatagatta ccgcgcctgt gtcgtactgg gtacagcgca cgcccggctg caccgtctgg
     5281 ctcattgtaa ttgccgaggt tctaaggtcg gagagcatct tctcgctctt tttgtagaac
     5341 tcaacagctt cggttatata aggattataa taaagggtgc tgtcgctgtc aaat
//
LOCUS       DAONVQ010000125         5305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_11378_length_5305_cov_4.425905, whole genome shotgun sequence.
ACCESSION   DAONVQ010000125 DAONVQ010000000
VERSION     DAONVQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11378_length_5305_cov_4.425905"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLD98_07675"
     CDS             <1..686
                     /locus_tag="PLD98_07675"
                     /inference="COORDINATES: protein motif:HMM:NF037850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06157.1"
                     /translation="VKVYKNNKLTEIVYPNRKLFPNLPHPPMTPRDFLGNALETDPDL
                     NKTSAKYFKEVLLAAETNGFNVPPSAYAKLLMAMISGKKTISETIDIITKYFANWGNV
                     QPEYKFEGYIDDKLVATVVKSPSNICKVTAKADKKVLVEDATYDVTRIELIAKDQNNN
                     RLPYAKNAINVTVEGAARIIGPSEFALLGGARAFWIRSIGKSGDIKVTIKGEGIDEPI
                     VLNLEAIKK"
     gene            838..1170
                     /locus_tag="PLD98_07680"
     CDS             838..1170
                     /locus_tag="PLD98_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQA06158.1"
                     /translation="MPVRISTEDNVVTAYLSGEIDHHSAAAIRDAIDTEIQIQRPKVL
                     RLDFSDVSFMDSSGVGLIMGRYKNALNFGGTTEVHNLPPFCERILKLSGLEKIIRFYK
                     ENRDASNK"
     gene            1154..1588
                     /gene="spoIIAB"
                     /locus_tag="PLD98_07685"
     CDS             1154..1588
                     /gene="spoIIAB"
                     /locus_tag="PLD98_07685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HQA06159.1"
                     /translation="MQVINEMKMVIDALSINEGYCRVAVATFVAQLDPTIDELNDIKT
                     SVSEAVTNCIVHAYPKKKGKIYITVRLYADGLARVIIKDKGIGIEDIKKAREPLFTTA
                     GGERSGLGFSVMESFSDRLLVRSKPGRGTSITLEKRIVGKGK"
     gene            1593..2264
                     /locus_tag="PLD98_07690"
     CDS             1593..2264
                     /locus_tag="PLD98_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQA06160.1"
                     /translation="MNEADKKKQALITENLGLVHACAARFKGRGVEYDDLFQAGCVGL
                     IKASEGFDESLGYAFSTYAVPAILGEIKRIFRDGGTVKIGRAAKEKARQLLKAKEELT
                     NQLDREPTISEIAEYSSMSVPETAELICAATPPLSLTAENEDNENQEMDIPVPSSDEG
                     ITDRLALHQVMQALDETDRNIIDLRYFKGLTQTVTAKKLGMSQVQVSRREKVILLKLR
                     ELLSS"
     gene            complement(2344..3567)
                     /locus_tag="PLD98_07695"
     CDS             complement(2344..3567)
                     /locus_tag="PLD98_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HQA06161.1"
                     /translation="MHLTKVKEGVFAVGILNPGMRVFDIVLRTEYGTSYNSYIVKGAE
                     KTALIDICHGSFTKAYLENVSSVVPPEEIDYIIVNHTEPDHTGALAEFIKHCPKAEVF
                     ASRAGAMFLKNITNNPDIKINAVNDGDELDLGGKTLKFISAPFLHWSDSIFTYLPEDK
                     LLFSCDFLGCHYCEPYVFDTNIKYAEGYEKALEEYYHAIMHPFAQHVRNGLKKIEGLE
                     IDAVCPSHGPILTKGNRLEFVIGKYTEWSKEVKRDKLLIPIFYVSAYGNTERIANAIK
                     EGIDSVFDGGAETETYNIIKHDMGALAKLINTCDAVAIGTPTINADALAPVWNLLAFV
                     EAVNSRNKPAIVFGSYGWSGEGVPNIIARLKGLRMAVEEQGLKICFVPSDEDIEKAKE
                     YGKEFALRVKEKLNK"
     gene            complement(3611..4993)
                     /locus_tag="PLD98_07700"
     CDS             complement(3611..4993)
                     /locus_tag="PLD98_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HQA06162.1"
                     /translation="MTNAYKATVNIVPSERQLNLKTHKFGAFVYFGMNTFTAADIGSG
                     FTSPKTFGPEDFSAENIVSVVKSAGMTSLVLTVKHYDGFCLWPSRQTDYTIAESNWMD
                     GGGDIVKAISDECARQGIAFGIYFPVWDKHEESYGSGEQYDNFVKGQLAELLTDYGDI
                     FMVWLDHTAGKGNNGKIQSSFDWNGYYKLIRLLQPNAIIAGHGPDARDVGNHRGVCRT
                     SEWSVVPKSLGPDENGLIHFGDTMKLDLGSRKAIKREKEFIWYPAVALVPMRPNWFYR
                     SEDDVVVKTKDKIIKLYYNSVGANTALYLGVAVDKRGRIPSVDSQILKNLGKDLKLMF
                     GYNLADSAEFSASSSLSELYSPERIKLQNGFWRPAEGDKKPELIIKLSEPDMFDKIVI
                     SENIANGQHVEKFRVYYKNEKNKWKLFYKGTVIGSERICTSKPVKSDEIKLVFEQYRD
                     FFEIFSVAIN"
     gene            complement(5012..>5305)
                     /locus_tag="PLD98_07705"
     CDS             complement(5012..>5305)
                     /locus_tag="PLD98_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HQA06163.1"
                     /translation="ELISVTGYTCPRGKTYAETEISNPQRVVTSTVKLEGGRNPVVPV
                     KTSKPIDKALIFDCMKEINKAIAKAPVKIGDVIIPNVLGTGADIVATAEDLGK"
BASE COUNT         1339 a         1267 c         1328 g         1371 t
ORIGIN      
        1 atgtaaaggt ttataagaac aacaagctga cagagattgt ataccccaac cgcaagctgt
       61 tcccgaacct tccccacccg cccatgactc cgagagattt cctcggcaac gcccttgaaa
      121 cagaccccga cctcaataag acctccgcga agtactttaa ggaagttctg cttgccgcgg
      181 agactaacgg ctttaatgta ccgccctccg catacgcaaa gctccttatg gcaatgataa
      241 gcggcaaaaa gacaatctcc gagaccattg acatcatcac aaagtacttt gctaactggg
      301 gcaatgttca gcccgagtac aagtttgagg gctatattga cgacaagctc gtggcaaccg
      361 ttgtaaagtc gccctccaac atctgcaagg tcaccgcaaa ggcggacaaa aaggtgcttg
      421 ttgaggacgc gacctatgat gtcacaagaa ttgagcttat cgcaaaagac cagaacaaca
      481 accgcctgcc ctacgctaag aacgccatta atgtaacggt tgagggcgcg gcaaggatta
      541 taggcccctc agagttcgct ctgctcggcg gagccagggc attctggata aggtcaatcg
      601 gcaagtcggg cgacattaag gtaactataa agggcgaggg aatagatgag cctattgtcc
      661 ttaatctcga ggcgataaag aaataaatcc aaagataaga aagcagggac tttaagccct
      721 gcttttttta attgcaacct gcacaaaaaa taaaaaatgg taaaattaga catttttcac
      781 acggacaagc ctctattata ggatagaggc tttaaatatt tttcaggagg taaaaccatg
      841 cctgtcagga tttctaccga ggacaatgtg gtgactgcgt atctctccgg cgagatagac
      901 caccattccg cagccgccat aagggatgcc attgatactg agatacaaat tcagcgtcca
      961 aaggttttaa ggcttgattt ttcggatgta agtttcatgg acagctcggg tgtcgggctg
     1021 attatgggca gatacaaaaa cgcgctgaat ttcggcggca caacagaggt tcacaatttg
     1081 cccccattct gcgaaaggat tctgaagctt tcggggcttg aaaagataat tcgcttttat
     1141 aaggagaata gagatgcaag taataaatga aatgaaaatg gtgattgacg ctctctcgat
     1201 aaacgaaggc tactgcaggg ttgccgttgc gacctttgtg gctcagcttg accccaccat
     1261 tgacgagctg aacgatatta agacatcggt cagcgaggcg gtcacgaact gcattgtcca
     1321 cgcctacccc aaaaagaagg gcaaaatcta cataaccgta agactctatg ctgacggtct
     1381 cgcaagagtg attatcaagg ataagggcat aggaatagag gacataaaaa aagcccgcga
     1441 gccgcttttc acaacggccg gcggagagcg ttcgggtctt ggattctcgg taatggagag
     1501 cttttcggac aggctgctgg ttcgctcaaa gcccggcagg ggcaccagca ttacgcttga
     1561 aaaacgaata gtgggaaagg gaaaataaaa taatgaacga ggccgacaaa aaaaagcagg
     1621 ccctgattac cgaaaatttg ggacttgttc atgcctgtgc cgcgcgattt aagggcaggg
     1681 gcgttgaata cgacgacctt tttcaggcag gctgcgttgg gctcataaag gcctctgagg
     1741 gctttgatga aagtctgggc tacgcctttt ccacttacgc ggtgcccgcc attctgggcg
     1801 agataaagcg gattttccgc gacggcggaa cggttaaaat aggcagagcc gcaaaggaaa
     1861 aggctcgtca gcttttaaag gctaaggagg agctgacaaa tcagcttgac agggagccga
     1921 cgatttctga gattgccgaa tacagctcaa tgagcgtacc ggaaaccgcg gagcttattt
     1981 gcgccgccac ccctcccctg tcccttacgg ctgagaacga agacaacgaa aatcaggaga
     2041 tggatatacc cgtacccagc tctgatgaag gaataaccga taggctggct ctgcatcaag
     2101 tcatgcaggc acttgacgaa acggacagga acataataga tttgcggtac tttaagggtc
     2161 ttacgcaaac cgttaccgct aagaaactgg gtatgtccca ggtgcaggtt tcaaggcggg
     2221 aaaaggttat actacttaag ctgcgagagc ttttaagctc ttaaagctgc agcggttctt
     2281 tccatatcgg ccgctgcgga gtgcataatg aaagcggtgg aggtgttcca ccgctttcgc
     2341 cttttattta ttcagtttct ctttaactct gagggcaaat tccttgccgt actcctttgc
     2401 tttttcgatg tcctcatcag agggaacgaa gcaaatcttg agaccctgct cttccaccgc
     2461 cattctgaga cctttaagtc ttgcgatgat gttcggaacg ccctcgccgc tccagccgta
     2521 ggagccgaat actatcgcgg gcttgttgcg gctgttgaca gcctcaacga atgcaaggag
     2581 attccaaacg ggagcaaggg cgtcggcatt gattgtgggt gtgccgatcg caaccgcgtc
     2641 gcaggtgttg atgagctttg cgagagctcc catatcgtgc tttatgatat tgtaggtttc
     2701 agtctcggcg ccgccatcaa atacggagtc gatgccctct ttaatggcat ttgcgattct
     2761 ctcggtattg ccgtaggcgg agacatagaa aatgggaatc agcagcttgt ccctcttaac
     2821 ctctttgctc cactcggtat atttgccgat tacgaactcc agtctgttgc cctttgtgag
     2881 aatgggtcca tgactggggc atactgcgtc tatctcaaga ccctcaatct ttttaagacc
     2941 gttccttaca tgctgggcaa aggggtgcat gatggcgtga tagtattcct caagggcttt
     3001 ttcatagcct tccgcgtact tgatgtttgt atcgaaaaca taaggctcgc agtagtggca
     3061 gccgaggaag tcgcatgaga acaggagctt gtcctcgggg agataggtga atatggagtc
     3121 ggaccaatga aggaacggcg cgcttataaa cttaagagtc ttgccgccga ggtccagctc
     3181 gtcgccgtcg tttacggcgt ttatcttaat atcgggattg ttggtgatgt ttttgaggaa
     3241 catagctccc gcgcggcttg cgaaaacctc tgccttgggg cagtgcttta taaactccgc
     3301 aagtgctccc gtatggtcgg gctcggtgtg gtttactatg atgtagtcga tttcctcggg
     3361 aggaacgacg gagctgacat tttcaagata agcttttgta aatgagccgt ggcagatgtc
     3421 gataagggct gttttctcgg ctcccttaac aatataagaa ttgtagctgg tgccgtactc
     3481 tgttctgaga acaatatcaa aaaccctcat gccggggttg agtattccaa cggcaaagac
     3541 gccttcttta acctttgtaa gatgcaaatt caacactcct tttttcggga tgagaataaa
     3601 atatcacatt ttagttaatt gcaacagaga aaatttcaaa aaagtccctg tactgttcga
     3661 aaaccagctt aatctcgtcc gattttacgg gctttgaggt gcaaattctc tcgctgccga
     3721 taactgtgcc cttgtaaaag agcttccatt tatttttctc gtttttgtag tataccctga
     3781 atttttccac atgctgcccg ttcgcgatat tttcgctgat tacaatcttg tcgaacatat
     3841 cgggctctga aagctttatt attagctccg gctttttatc gccctcggcg ggacgccaga
     3901 agccgttttg aagcttaatt ctctctgggc tgtaaagctc gctgagcgac gagcttgcag
     3961 aaaactctgc gctatcggca aggttatagc cgaacatcag ctttaaatcc ttgcctaaat
     4021 ttttaagtat ctggctgtcg actgaaggga ttctgccccg cttgtcgacg gctacgccta
     4081 aatacagcgc ggtgttggct ccaaccgaat tatagtagag cttaatgatt ttatccttgg
     4141 ttttgaccac cacatcgtct tcgctgcggt agaaccaatt gggtcgcatg ggaacaaggg
     4201 cgacagcggg ataccagatg aactcctttt cacgcttgat agccttgcgg ctgcccaaat
     4261 ccagcttcat agtgtcgcca aagtgaatta agccgttctc gtcgggtcct aaggacttgg
     4321 gcacaacgct ccattcgctt gttctgcaga ctcccctgtg gttgcccaca tcccttgcgt
     4381 cggggccgtg ccccgctatg attgcgttgg gctgcaggag cctaatcagc ttatagtagc
     4441 cgttccagtc aaaactgctt tgaatcttgc cgttgttgcc cttgcctgcg gtgtggtcaa
     4501 gccagaccat gaatatatcg ccgtaatcgg ttaaaagctc cgccagctga ccctttacaa
     4561 aattgtcgta ctgctcgccg ctgccgtagc tttcctcgtg cttatcccag acgggaaaat
     4621 aaatgccaaa ggctattccc tgacgagcgc actcgtcaga aatagccttg acaatatctc
     4681 cgccgccgtc catccagttg gactcggcga ttgtgtaatc ggtttgtctt gaaggccaca
     4741 gacagaatcc gtcgtaatgc ttgacggtca gcacaaggga ggtcatcccc gcgcttttta
     4801 cgacggatac tatgttttcg gcggaaaagt cctcgggccc aaaggtcttt gggcttgtaa
     4861 agccgctgcc tatgtcggct gcggtaaagg tattcattcc gaaatacaca aaagctccga
     4921 acttatgtgt tttcagattg agctgtcttt cacttggaac tatgtttacc gtagctttat
     4981 aagcatttgt cataaaggcc tcttttcgaa attattttcc taaatcctca gcggttgcca
     5041 caatgtccgc gcctgtgccg aggacattgg gaattatgac atcgccgatt ttaacgggcg
     5101 ctttggcgat tgccttatta atctctttca tgcagtcaaa aatcagtgcc ttgtctatgg
     5161 gcttgctggt cttgacgggc acgacggggt ttctgccccc ttcgagctta actgtggagg
     5221 taactactct ttggggattt gagatttccg tctcggcgta ggttttgcct ctggggcagg
     5281 tataacccgt gacggagata agctc
//
LOCUS       DAONVQ010000126         5202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_11629_length_5202_cov_4.235088, whole genome shotgun sequence.
ACCESSION   DAONVQ010000126 DAONVQ010000000
VERSION     DAONVQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11629_length_5202_cov_4.235088"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLD98_07710"
     CDS             complement(<1..488)
                     /locus_tag="PLD98_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HQA06164.1"
                     /translation="MLKLDEILRLVQKPARYTGGEVNAVVKSKDDVQIRYAFCFPDLY
                     EIGMSHLGMKILYHLANSREDTWCERVFAPAKDMEELMRQNGIKLFGLESGDPISDFD
                     LIGFTLQYELCYTNVLNMLDLAGLPVYSKDREELLPVVVAGGPCVCNPEPLADFIDIF
                     LPG"
     gene            complement(569..865)
                     /locus_tag="PLD98_07715"
     CDS             complement(569..865)
                     /locus_tag="PLD98_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HQA06165.1"
                     /translation="MDNCCLNDLFRKEVIDIDSGKKYGCVCDVEVNVCSGRLTALILF
                     GKYKMWGFGGREDDLRIPWEDIVVIGDETILVKMDRCSRIKPPKGKKGMDSFFR"
     gene            complement(1004..1522)
                     /gene="folK"
                     /locus_tag="PLD98_07720"
     CDS             complement(1004..1522)
                     /gene="folK"
                     /locus_tag="PLD98_07720"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HQA06166.1"
                     /translation="MKTAYIALGSNLGDREHYLFSALKALSLLNGTHLKKVSKVYETE
                     PVGCVDPEPYLNMCAEIETELSPLALLGALLGIEAANGRERPYTNCPRTLDLDLLIYE
                     NERMNTRELTIPHPRMAERAFVLVPLSDIKANLKFGEFDFSSEYEKLSKDGVYEYKLK
                     NKELISLIESLA"
     gene            complement(1519..1902)
                     /gene="folB"
                     /locus_tag="PLD98_07725"
     CDS             complement(1519..1902)
                     /gene="folB"
                     /locus_tag="PLD98_07725"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HQA06167.1"
                     /translation="MDKIIIKNLRLYAYHGVNPEEKSQGQPFFIDIEAFTDLKRASMT
                     DDISDTVSYSKITKTVRKIFLERSWDLIEKAAGKIARGVLKEFPTLKAVTVRVKKPEA
                     PVKADFEYMAVEINLSRDELYGVRE"
     gene            complement(1908..2762)
                     /gene="folP"
                     /locus_tag="PLD98_07730"
     CDS             complement(1908..2762)
                     /gene="folP"
                     /locus_tag="PLD98_07730"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQA06168.1"
                     /translation="MLTAETKKFRFRDKSLTLGERTYIMGILNVTPDSFSDGGLYYQQ
                     KEAVNRARQMLAEGADIIDIGGMSTRPFSEPVSEEEEIKRLIPVIKEVRAENPYAIIS
                     ADTYRINVAEKALRAGADIINDVSGEFKHLLAELIKDFDAGWIIMHSGGVPSGEFPIY
                     EKGVVREVADFFDDMLEKITAFGIDKSRVCLDPGFGFAKGVEENLQLLKNLATFKKED
                     IALLVGVSRKRFIGAMMEEVDPTQRLEGTLAANFRAVELGADIVRVHDIAEHKRFFKA
                     ADFLLRKG"
     gene            complement(2793..3353)
                     /gene="folE"
                     /locus_tag="PLD98_07735"
     CDS             complement(2793..3353)
                     /gene="folE"
                     /locus_tag="PLD98_07735"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HQA06169.1"
                     /translation="MDTEKIKKAVRDILEAIGEDPDREGLIDTPARVARMYEEVFSGL
                     REDPKMHIKLFDEPMHSEMVTVRDIPLYSMCEHHLLPFVGKAHIAYIADGKVIGLSKL
                     ARIVNTFARRPQLQERLTADIADFLERELSPKGVAVVIEAEHLCMTMRGIKAAGSLTQ
                     TSALRGIMRSDARSRAEVMTLLKGGK"
     gene            complement(3426..4589)
                     /locus_tag="PLD98_07740"
     CDS             complement(3426..4589)
                     /locus_tag="PLD98_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HQA06170.1"
                     /translation="MSRKTYALISILLITAVLASLFFFTDARSFLSSIMSDRFDRELN
                     ENPYVFYRHYFNELNENEKLAYINIYNKIGNFPERIEIPQLSEAELDRVFKAIRYDNP
                     DLIYIGNTCQMLAVSGRYYFIPLYSMGKDEAKAKLQELKAKIESVVSSMPEGLDEYSK
                     ELYLHDYLVANCTYVDKEGADTAYSALIKGEAICSGYANAMQLLLSKAGIKSTIITGV
                     ATDDQGYPQSHMWNIVYIDGRPYHLDATWNDPVPDTKGNVRHMYFNVNDSYIAVSHSD
                     FNISIPCNDIADNYYKRQGQSFDRYDDGTRARITLKLKENIEQGKMQTELRFENEKAF
                     SAAVSSLLNKGEIYDILRSVNTEHGINISTNAIKYSLNERLMIMVIIAEGKAS"
     gene            complement(4602..>5202)
                     /locus_tag="PLD98_07745"
     CDS             complement(4602..>5202)
                     /locus_tag="PLD98_07745"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HQA06171.1"
                     /translation="SCTGTSGEITFAAFIKADFENRKITVTALDPASAADANGFEGKL
                     TEHYQRAGIRQLTLAAESITGFKADRYISFTEANFKRILRAIGDITVNVHRDIYLSGS
                     DVILELKAGEQKLSPDTLLKYIKYGEKGNALIPLQEKVISYIVKSLLTPQSAAKIDRI
                     FSDTINLVETNISVMDFNENKPVFELFSRESVQYANLSS"
BASE COUNT         1196 a         1268 c         1220 g         1518 t
ORIGIN      
        1 ccgggaagga aaatgtctat aaagtcagcc agaggctcgg gattgcacac gcaggggccg
       61 cccgctacca cgacgggcaa aagctcctct ctgtcctttg agtaaacggg gagacccgca
      121 aggtccagca tattcagcac attcgtgtag cacagctcat actgaagggt aaagcctata
      181 aggtcaaaat ccgaaatcgg gtcgccgctt tcaagaccaa agagctttat gccgttttgg
      241 cgcataagct cctccatgtc ctttgcgggg gcaaaaaccc tctcgcacca tgtgtcctcc
      301 cttgagtttg caaggtggta gagaatcttc atgccaaggt gggacatgcc gatttcatat
      361 aggtcgggaa agcaaaaggc gtagcggatt tgcacatcgt ctttgctttt tacaacggcg
      421 tttacctcgc cgcccgtata tctggcaggc ttttgaacga gcctgagtat ttcgtctaat
      481 ttaagcattt tggaattcct ttcggtcctt gttttcgcga ataattgtat cacaacgcgg
      541 ggctgattac aaattttatg tgctcggctt atctgaaaaa gctgtccatg ccctttttgc
      601 cctttggggg cttaatgcgg ctgcagcggt ccatcttaac aagtatcgtc tcatcgccta
      661 tgaccacaat atcctcccaa gggattctaa ggtcatcctc cctgcctcca aagccccaca
      721 tcttgtattt gccgaaaagt attaaggcgg tcagtctgcc gctgcagaca tttacctcca
      781 catcgcagac gcatccgtat tttttgcccg aatctatatc tatgacttct tttctgaaca
      841 aatcgtttaa acagcagttg tccatatgta aatcctgacc tttcggataa attgagggaa
      901 ataacaataa caccccaatc tattttattc cgccgtcttt gcgatatgat aaagttcgga
      961 caaccgctga cgaataaaaa gaaaaaaccg cattaaccgc ggtttaagca aggctttcaa
     1021 ttaaggagat aagttctttg ttttttaatt tgtactcata taccccgtct tttgaaagtt
     1081 tttcatattc tgaggagaaa tcgaattcgc cgaatttgag atttgctttt atatccgaca
     1141 aggggacaag gacaaatgcc ctttccgcca ttctggggtg agggatagtc agttcccttg
     1201 tgttcattct ctcgttttcg tatatcagaa ggtccaaatc aagggttctg gggcagtttg
     1261 tataaggtct ttccctgccg ttcgcggctt caatccctaa aagggcgcca agaagggcga
     1321 ggggggaaag ctcggtttca atttctgcgc acatattaag atagggctca gggtcaacgc
     1381 agcccacggg ctcggtttcg tatacctttg aaactttttt tagatgagtt ccgttcagta
     1441 aagaaagggc tttcagcgcg ctgaacagat aatgctccct gtcgcccaaa ttggagccca
     1501 gggcgatgta tgcggttttc actctctcac cccataaagc tcatctctgc taagatttat
     1561 ttccaccgcc atatattcaa agtccgcttt tacgggagct tcgggctttt taaccctgac
     1621 cgtaacggct ttgagggtcg ggaactcttt taaaactccc cttgctatct tccccgccgc
     1681 cttttctata aggtcccagc ttctctccag aaaaattttg cggaccgttt ttgttatctt
     1741 cgaatagctg acggtgtcgg aaatatcgtc ggtcatcgaa gcccgcttta agtcggtaaa
     1801 agcctcgata tcaataaaaa agggctgacc ctgagatttc tcttcagggt tcactccgtg
     1861 ataagcataa agccttaggt ttttgattat gattttatcc atgcccttta cccttttctt
     1921 aatagaaaat cggctgcctt aaaaaagcgt ttgtgctccg ctatgtcatg cactcttaca
     1981 atgtcggcgc caagctccac agccctgaaa ttcgcggcaa gagttccctc aagcctttgc
     2041 gtcgggtcaa cctcctccat cattgcgccg atgaacctct ttctcgaaac gcccacaaga
     2101 agggcgatgt cttctttttt aaatgtggcg aggtttttca gaagctgaag gttttcctca
     2161 acgcctttcg caaagccaaa gccggggtca aggcagacgc ggcttttgtc aatgccgaag
     2221 gcggtgattt tctcaagcat atcgtcaaag aagtcggcaa cctcacgcac tacgcctttt
     2281 tcatatatgg ggaattcgcc gctgggcact ccgcccgagt gcattataat ccagccggcg
     2341 tcaaaatcct taataagctc agccaaaagg tgcttaaact cgccgctgac atcgtttata
     2401 atgtccgctc ccgctctgag agccttttcc gccacattga ttctgtatgt gtcggcggag
     2461 attattgcgt aggggttttc cgccctcacc tctttaatga cggggatgag ccttttgatt
     2521 tcctcctcct cgcttaccgg ctcgctgaag gggcgggttg acatgccgcc gatgtcgata
     2581 atgtcggcgc cctcggcaag catttgcctt gccctgttta cagcctcttt ttgctgatag
     2641 tagagaccgc cgtcggagaa ggagtcgggg gtgacattga gtattcccat tatgtatgtt
     2701 ctctcaccca aggtaagaga tttgtctctg aaacgaaact tttttgtttc cgcggtcagc
     2761 atataatcac cccatcagct aatttactga aattattttc cgccctttaa aagggtcatg
     2821 acctccgccc ttgaccttgc gtcgctgcgc attatgcctc tgagcgcgct tgtctgtgta
     2881 agacttcccg cggcctttat gccccgcatt gtcatgcaaa ggtgctcggc ctctatgacg
     2941 accgccacgc ccttggggct cagctccctt tcaagaaagt ccgctatgtc ggctgtgagc
     3001 ctttcctgca gctgaggtct gcgggcaaag gtgtttacta tccttgcaag ctttgagagc
     3061 cctattacct tgccgtcggc tatataggct atatgagcct taccaacaaa gggcaaaagg
     3121 tgatgctcgc acattgagta aaggggtatg tccctgacgg ttaccatctc gctgtgcatg
     3181 ggctcgtcaa agagctttat gtgcattttc gggtcctctc tgagccccga aaaaacctcc
     3241 tcatacatcc ttgcgactct ggcgggggtg tcaatcagac cctctctgtc agggtcctcc
     3301 cctatcgcct caagaatgtc ccttaccgcc ttttttatct tttcggtatc tattttctgc
     3361 tccaaaggat agtttttttg agcgtcgtac aattaaatca cttgcctttc gatacgggaa
     3421 agaggtcagc tcgccttgcc ctctgctata atcaccatta tcataagcct ttcgttaagg
     3481 ctgtatttga ttgcgtttgt gctgatattt atgccgtgct cagtattcac gcttcttaaa
     3541 atatcgtaaa tctcaccctt gtttaaaagg gagctgaccg ccgcggagaa tgccttttcg
     3601 ttttcaaatc gcagttcggt ctgcattttg ccctgctcga tgttttcttt gagcttgagc
     3661 gttattctcg ctcttgttcc gtcgtcgtag cggtcaaagc tctgaccctg ccttttgtag
     3721 taattgtcgg cgatgtcgtt gcagggaatt gagatattga aatccgagtg ggagaccgct
     3781 atgtagctgt cgtttacatt aaagtacata tgccttacat ttcccttggt gtcgggaacg
     3841 gggtcgttcc atgtggcgtc aaggtgatag ggtctgccgt ctatgtaaac tatgttccac
     3901 atatggcttt gggggtagcc ctggtcgtcg gtggcgacgc ccgtaattat tgtgcttttt
     3961 atgcccgctt tgctgaggag aagctgcatg gcgttcgcgt agcctgagca gatcgcctcg
     4021 ccttttatta aagctgaata agcggtgtcc gccccctctt tgtccacata ggtgcagttc
     4081 gcgacaagat agtcgtggag ataaagctcc tttgagtatt cgtcaagacc ctcgggcatt
     4141 gaggagacaa cagactctat tttggcttta agctcttgga gtttcgcttt tgcctcatcc
     4201 ttgcccatgg aataaagggg gataaagtag tacctgcccg aaaccgcaag catttgacag
     4261 gtgttgccta tgtagataag gtcggggttg tcgtagcgta tggctttgaa aaccctgtca
     4321 agctccgctt cggaaagctg agggatttct attctttcgg ggaaattgcc tattttattg
     4381 tagatgttta tatacgccag tttttcgttc tcgttaagtt cattgaaata gtgtctgtaa
     4441 aagacatagg ggttttcatt taactccctg tcaaagcggt cggacataat tgagctcaaa
     4501 aaactgcggg catccgtaaa aaagaaaagg gatgccaaaa cagctgtgat taacaatatg
     4561 gaaataaggg cgtaagtctt tctgctcatt tttactccaa actatgaact gaggttagcg
     4621 tattggacgg attctcttga gaaaagctcg aaaaccggct tgttctcgtt aaaatccatt
     4681 acggaaatat tggtttcaac aaggttaatg gtgtccgaga aaatgcggtc aattttagcg
     4741 gcgctctggg gagtcagcag agattttaca atatagctga ttactttttc ctgcaaagga
     4801 atgagggcgt ttcccttttc gccgtacttt atatacttta gcagcgtgtc gggggagagc
     4861 ttttgctccc ctgctttaag ctccaaaatg acatctgagc cggaaaggta aatatccctg
     4921 tggacattta ccgtaatatc gccgatggcg cggagaatcc ttttgaaatt agcctccgtg
     4981 aaagaaatat atctgtcagc cttaaagcct gtaatgcttt cggcggcaag ggtcagctgc
     5041 cttatgcccg cccgctggta gtgctccgta agctttccct caaagccgtt tgcgtcggcg
     5101 gctgatgcgg ggtcaagggc ggtgacggtg atttttctgt tttcaaaatc cgctttaata
     5161 aaggcggcga aggtaatctc ccccgaggtg cccgtgcagg ag
//
LOCUS       DAONVQ010000127         5198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_11640_length_5198_cov_5.260937, whole genome shotgun sequence.
ACCESSION   DAONVQ010000127 DAONVQ010000000
VERSION     DAONVQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11640_length_5198_cov_5.260937"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLD98_07750"
     CDS             complement(<1..842)
                     /locus_tag="PLD98_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HQA06172.1"
                     /translation="MIHKYELNGYKIVIDPCSGAVHLFDDTAYDIVSLIKAAPMSPDC
                     PEDIVNSLSEKYSTDSIKEAYSDIYALYREGTLFSEDDYAEFAEMLGSAPVKSMCLNV
                     AHDCNLKCEYCFAAQGDFGMEKGLMPFEVAKSAIDFLIKHSGGRRNLELDFFGGEPLM
                     NFDVVKKTVEYARSVEKEHGKNFRFTLTTNGLLLNDEIIDYLNREMYNLVLSLDGRKE
                     VNDKLRPTRSGSGSYDIIVPKYQKLIEKRGDGQYYVRGTFTRHNLDFADDLLHLADDL
                     GFDQL"
     gene            complement(843..983)
                     /gene="scfA"
                     /locus_tag="PLD98_07755"
     CDS             complement(843..983)
                     /gene="scfA"
                     /locus_tag="PLD98_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HQA06173.1"
                     /translation="MKHIKTINSANLKKSTEKGGCGECQASCQSACKTSCTVANQECE
                     RK"
     gene            complement(1063..1572)
                     /locus_tag="PLD98_07760"
     CDS             complement(1063..1572)
                     /locus_tag="PLD98_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQA06174.1"
                     /translation="MAESMKPAASYSSKKDNRNIVSVIIILVCVAVAGIPLGVFLGKK
                     LPKQASDTTTTQPQTTEAATETQTTVTTPNVPLYPTGLYTVATESSNLNLRSGPGSEF
                     PTINSIPKGTVLTVVEVQGDWGRVDYKTTIGWVNMKYMKAANPDETTTAESTSTTAAT
                     GVTETAANQ"
     gene            complement(1718..2164)
                     /gene="nifU"
                     /locus_tag="PLD98_07765"
     CDS             complement(1718..2164)
                     /gene="nifU"
                     /locus_tag="PLD98_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HQA06175.1"
                     /translation="MLYSDKVMDHFMNPRNVGKIDDADGVGEVGNAKCGDIMKVYIKV
                     DNDIITDVKFNTFGCASAIATSSIATEMIKGKPISEALKLTNKAVVEALDGLPPVKIH
                     CSVLAEEAVKAAIADYYKKIGKEPDPEIFSCTGCCESCGAEDAHSH"
     gene            complement(2164..3342)
                     /gene="nifS"
                     /locus_tag="PLD98_07770"
     CDS             complement(2164..3342)
                     /gene="nifS"
                     /locus_tag="PLD98_07770"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HQA06176.1"
                     /translation="MLIYADNAATTAVSKKALEAMLPYFTEHYGNASSIHRVGQEANK
                     ALQQARAEIAAILGAEPKELIFTSGGSESDNQAIISAALQGAKKGKKHIISSKIEHHA
                     VLHVLDKLEKEGYDITLLDVSPEGLVSPESVEKAIREDTALVTIMFANNEIGTIEPIK
                     EIGEICRKHGVIFHTDAVQAVGHVPINVKEMNIDMLSFSAHKFHGPKGIGGLYVRKGI
                     RLLPLIEGGAQERGLRAGTENVAAVVGMAAALKEQVENMEKNAEKLTKMRDRLIDSIL
                     KEVPRVRLNGDRVKRLPGNVNFCFEGIEGEALLLMLDNLGICGSSGSACTSGSLDPSH
                     VLLAIGLPHEIAHGSLRLTFSEYNTEEEIDYIIKVIPQVVKRLRDMSPMWERIVKEEA
                     "
     gene            3964..>5198
                     /locus_tag="PLD98_07775"
     CDS             3964..>5198
                     /locus_tag="PLD98_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQA06177.1"
                     /translation="MLLTGAEIIIETLIEQGCDTVFGYPGGFVLNIYDELYKNSDRIK
                     HIITAHEQGAAHAADGYARATGKVGVVIATSGPGVTNLVTGIATAFLDSTPIVAITGN
                     VPLSMLGRDSFQEVDSTGITMPITKHNYIVKDVKDLASIIREAFVVAKSGRPGPVLVD
                     VPKDIQLAKCEFEPVGMLEAEPKRKVSDNFLSKCAALINASERPYIYCGGGVIAADAS
                     EEIKALSLKLGAPIGLSIMGLSSIPRDFPNWLGMMGMHGRYSSTKANYEADLIIALGV
                     RFSDRATGDKNKFKRDAKIIHIDIDPAELNKNIDSTISSAGDIKNAVGRLLPLIEDKD
                     RTPWQRRIDELRADEEKYAPQNVGRLTPQKIIAALQNCTSKDTIVVTDVGQHQMWAAQ
                     YYKLYKPRTLLTSGGLGTMG"
BASE COUNT         1339 a         1243 c         1199 g         1417 t
ORIGIN      
        1 agctggtcaa agcccaaatc gtcggcaagg tgcagaaggt catcggcaaa gtcaaggtta
       61 tgccttgtaa aggtacccct tacatagtac tgaccgtcgc cccttttctc tatgagcttt
      121 tgatatttcg gcactattat gtcatagctg ccgctgccgg agcgggtggg tctgagtttg
      181 tcgtttacct cttttctgcc gtcaagggag agcaccagat tatacatctc cctgttaagg
      241 tagtcgatta tctcatcgtt caaaagaagt ccgttggtgg taagggtgaa cctgaagttc
      301 ttgccgtgct ccttttctac gctccttgcg tactccactg ttttcttaac gacatcaaag
      361 ttcatcagcg gctcgccgcc aaagaagtcc agctcaaggt ttcgccttcc gcccgagtgc
      421 tttataagaa agtcaatcgc gctctttgcg acctcaaagg gcataagtcc cttttccatt
      481 ccgaaatcgc cctgagcggc aaagcagtat tcgcatttaa gattgcagtc gtgggcaaca
      541 ttcaggcaca tggacttaac gggagcggag cccagcattt cggcgaattc agcataatcg
      601 tcctcgctga aaagcgtgcc ctcgcggtaa agagcataaa tatccgagta tgcttcttta
      661 atgctgtcgg tcgagtactt ctcggaaagc gagtttacaa tatcctcagg gcaatcaggg
      721 gacatgggag cggcttttat taaggaaaca atatcataag ccgtgtcatc aaaaagatgc
      781 acggctccgc tgcaagggtc gattacaatt ttgtatccgt tgagttcgta cttatgaatc
      841 aattattttc tctcgcattc ctgatttgct actgtgcagg atgttttgca agccgactgg
      901 caggatgcct ggcactcgcc gcagccgccc ttttcagtgc tctttttaag gtttgcggaa
      961 ttgatagttt tgatgtgttt cataattgtc ctccgaatta cgctgtattt ctacagtact
     1021 ataacacaag agaccaaata tttcaagtgt atacaagcta ttttattgat tggctgccgt
     1081 ttcggttaca ccagtggccg cggttgtgct ggtagactca gcggtagtag tctcatcggg
     1141 atttgccgcc ttcatgtact tcatgtttac ccagcctatt gtggtcttat aatcaaccct
     1201 gccccagtcg ccctgaacct ctaccacggt cagaactgtg cctttgggga tactgttgat
     1261 cgtggggaac tcgctgccgg gacctgagcg gagattcaga ttgctcgact cggtcgcgac
     1321 tgtataaaga cctgtgggat aaagaggaac attcggagtt gtaaccgttg tctgcgtttc
     1381 cgttgccgcc tcggttgttt ggggctgagt tgtggttgtg tcagatgcct gtttgggcag
     1441 ctttttgccg aggaaaactc cgagaggtat tcccgctacg gcaacgcata caagaattat
     1501 aataacgctt acaatattcc tgttatcttt ctttgagctg tagcttgcgg caggcttcat
     1561 tgattcagcc atgatgaacc tccaaaaaat gaattcagta aaatttatta aaagtatcat
     1621 cagtatatta gtattgtaat aaaaactgga aattatttca acttacaaaa ggtaacaaaa
     1681 aaaatccccg caaaacgggg acttttcttt tgttttctta gtgggaatgt gcgtcctctg
     1741 cgccgcagga ttcgcagcag cccgtgcagg agaaaatctc cgggtcgggc tctttgccga
     1801 ttttcttgta atagtcggcg atagcagcct ttactgcctc ctcagcgaga acggagcagt
     1861 gaatctttac cggcggcaga ccgtcaagag cttcaacaac cgccttgttg gtgagcttga
     1921 gagcctctga aatgggcttt cccttaatca tttcggttgc gattgagctt gtcgcaatgg
     1981 cagaggcgca gccgaatgta ttgaatttaa catcggtgat tatatcgtta tcaaccttga
     2041 tgtaaacctt cataatgtcg ccgcactttg cgttgccgac ttctccgaca ccgtcggcgt
     2101 cgtcaatctt gccgacattc ctcggattca taaaatggtc cataacctta tcgctgtata
     2161 acattatgct tcctccttaa caattctctc ccacataggc gacatatctc ttaatcgctt
     2221 tacaacctga gggataacct taattatata gtcaatttct tcctctgtat tatattcact
     2281 gaatgtcagt ctgagtgaac cgtgcgcaat ttcatggggc aaaccgattg cgagaagcac
     2341 atggctgggg tcaagggagc ccgatgtgca ggctgagccc gatgaaccgc agatgccgag
     2401 attgtcaagc attaagagca gcgcttcgcc ctcaattccc tcaaagcaga agtttacatt
     2461 gccggggagc ctttttactc tgtcgccgtt aagtcttact ctcggaacct ctttaagaat
     2521 gctgtcaatc agcctgtcgc gcatctttgt gagcttctcg gcgttcttct ccatgttttc
     2581 gacctgctct tttaaagcgg ctgccatgcc cactacagcg gcaacatttt ccgtgccggc
     2641 tctgagccct ctttcctgag cgccgccctc aatcaaaggc aggagcctta ttcccttgcg
     2701 gacataaaga cctcctatgc ctttggggcc gtggaacttg tgagcggaga aggagagcat
     2761 gtcaatgttc atttccttaa cattaatcgg aacatgtccc actgcctgaa cagcgtcggt
     2821 atggaaaatg acgccgtgct ttctgcagat ttcgcctatc tccttaatcg gctcaatggt
     2881 gccgatttcg ttgttggcga acatgattgt tacaagggca gtgtcttctc ttatagcctt
     2941 ctccacgctt tcgggagaaa caagtccctc gggcgaaaca tcgaggagag taatatcata
     3001 accttccttt tccagcttgt caagcacatg aagaacagca tggtgctcaa tcttggagga
     3061 aatgatgtgc ttcttgccct ttttagctcc ctgcagtgct gcggagataa tggcctggtt
     3121 gtccgattcg ctgcctccgg aggtaaaaat cagctctttg ggctccgcgc cgagtatggc
     3181 ggcgatttca gcccttgcct gctgcaaagc cttgtttgcc tcctgaccta ctctgtgtat
     3241 gcttgaggcg ttgccgtaat gctccgtaaa gtagggcagc attgcctcaa gtgccttttt
     3301 gctcactgcc gttgtggcag cattgtcagc atatatcaac atatctaaac ctccacggga
     3361 taaccgaatt aatttcctat cactttacta tggaattatt atacccccat tatttaacac
     3421 tgtcaatatg tcataagtca agaaaaaaca aaaattttgg tgctccgaat ggttaaaacg
     3481 catatactta aataataaac ggaacgcagt ccgattgtag tatttatgct ttaaatgtct
     3541 aatattatag ataattaagc ttttttttag cagattccat caacttttcc ataatgactg
     3601 tttattggta atgtaagatt atgcaatata tacaattgac atagggcccg ttttatgtaa
     3661 aaatatatcc aatcatttaa catttattgg agcgaaaatc gatgttaagt atagctgcaa
     3721 taattattat aataatcgtc attatactac ccgtgctatt agtaccggta gtgattattg
     3781 acgatttttt ttgcgctgta cttagattct cgcaatgtta taagcgttac acaagttaag
     3841 ctgtaactga atttacaaat tgtgacagaa tcttacagtg caaaaactgt aagatttttt
     3901 tattaccttc aagcgacatc actttaaagc gataaaaaaa gatattgtga aaggatgata
     3961 attatgctgc ttaccggagc cgaaatcatt atcgaaacat taatagaaca gggctgtgac
     4021 accgtgtttg gttatccggg aggcttcgtc ctgaacatct atgatgagct atacaagaat
     4081 tcggacagaa ttaagcatat aatcacggcg cacgagcagg gagccgccca cgcggcggac
     4141 ggctacgcaa gagccacggg taaggtaggc gttgtaatag ccacatcggg tcccggcgtg
     4201 acaaatcttg ttacgggtat cgcaaccgct ttcctggatt caaccccgat agtcgcaatc
     4261 acaggcaatg tacccctttc aatgcttgga cgcgacagct tccaggaggt tgacagcacg
     4321 ggaatcacaa tgcccatcac caaacataac tacatcgtaa aagatgttaa ggatttggca
     4381 agcattatca gagaggcatt cgtggtcgcg aagtccggca gacccggccc tgttcttgtc
     4441 gatgtgccca aagacattca gcttgctaaa tgcgagttcg agcctgtagg aatgcttgag
     4501 gctgagccga aaagaaaggt atccgacaac ttcctttcaa agtgcgcggc attaatcaac
     4561 gcatcggagc gtccctacat ctactgcggc ggcggagtga tagccgcaga cgcctcggag
     4621 gaaataaagg ctctctcgct taaactcggc gcaccgatag gtcttagcat catgggactt
     4681 tcctcaattc cccgtgattt ccccaactgg ctgggaatga tgggcatgca cggccgctac
     4741 agctccacca aggcaaacta tgaggcggat ttgattatag ccctcggcgt aagattcagc
     4801 gacagagcaa cgggcgacaa gaacaaattc aagagagacg ccaaaatcat tcacatagac
     4861 atcgacccgg cggagcttaa taagaatatc gactccacca taagctcagc gggagacata
     4921 aagaacgcgg tgggaaggct cctgcccctt atagaggata aggacagaac accctggcag
     4981 agaagaattg acgagcttag agccgacgaa gaaaagtacg cgccgcaaaa tgtcggcagg
     5041 ctcacacccc aaaagattat cgcggcgctt caaaactgca caagcaaaga cacaatcgtc
     5101 gtaaccgatg taggtcagca ccagatgtgg gccgcccagt actacaagct ctacaagcct
     5161 cgcacgctgc tgacttccgg cggactcgga acaatggg
//
LOCUS       DAONVQ010000128         5152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_11765_length_5152_cov_4.923484, whole genome shotgun sequence.
ACCESSION   DAONVQ010000128 DAONVQ010000000
VERSION     DAONVQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11765_length_5152_cov_4.923484"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1890)
                     /locus_tag="PLD98_07780"
     CDS             complement(<1..1890)
                     /locus_tag="PLD98_07780"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HQA06178.1"
                     /translation="MKIKCVLLSIILILMTLLPACSVGGGATAIKADTGTVLSPKAAD
                     PRYHTIDLGSLKKVASSGLLELYIDEVSLAVAVKETTGGKMWLSMPVENNTYASVLSL
                     NVLTKNGILYLNSQDNSVAMGGAGFEQTENGVKINYVFSLDENTAKIFLKGNKDAVIK
                     AEVEFTLSDGNFYFTADCSKVTASDGLYIERLNPLPYLGSTFGGAGEDYFLLPDGPGA
                     LMFTGKVDDSTNFVNLPVYGPDPASLYSVSADTSPTVIMPVFGVKDSDNAFAAIIEDG
                     DALAEIQARRASALSPARVSASFVLRSQNKLDKKSYISEQYDGNIKICYRFISGKSAN
                     YSGMARAAREQLIREGVLSTKKVNYTDKFPFNLSVIGAGKKKSGFYQSLTKIDQAQDL
                     AEILKGKGIDSINMRYSGLLSGGIVQHDSAKASVLKTLGGKSALERLSSYLERQKSGL
                     YIDVNVITSGGLKGYLSSFCARTSSGDVLTIENQNPLYPHVGAKTFYTEGLKLEKLEV
                     KIVDLLTNLKDTSFTGFSIKDAGKLLYSDFGGGYINRQEAKKLVASHIQALATQRGLM
                     VDTGNFYLVKNADIIVNLPVSTYYKESAAYEPVPAAPIILHGIADYSGKPVNLSGDRQ
                     KAMLKS"
     gene            complement(2042..2926)
                     /locus_tag="PLD98_07785"
     CDS             complement(2042..2926)
                     /locus_tag="PLD98_07785"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQA06179.1"
                     /translation="MSRQLEFLIGDEFDGKKIQSFLRGHLKMSARLVTSLKRIENGIL
                     LNGCHARTIDIMKKGDVLTLSIPDEEPTVIPSGEMPEIIYEDSDILVINKPALMPMHP
                     SHNHLEGTLANSVAAYLLSKGKAAAFRAVGRLDRGTSGLVICALNRFAAAALSGNVEK
                     EYLCIAEGIFEGSGTIDKPIYKPDPMKTLRAAGERKDAVPAVTHWESVLTGKGHSLLR
                     IRLETGRTHQIRVHFASMGAPLAGDDMYGGSTELISRPALHCSKASFIHPVTRASLTF
                     CVPVPEDFEKLREYISSP"
     gene            3179..4810
                     /locus_tag="PLD98_07790"
     CDS             3179..4810
                     /locus_tag="PLD98_07790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQA06180.1"
                     /translation="MSNVIIPRAESPEAVGVSSKCILDLIEDFKNSDIEMHSLMVIRH
                     GKVAAECYNYPFSAERPHACYSISKSITSTAIGFAVSEGILSLDTRLIDIFPEYKEKA
                     DENLKQLTIEHLVSMRSGKNTDILKNKAKIDWIDDYMKSPWYAKPGTDFRYVNENIYM
                     LSASLHRLTGMNMRDFLRPRLFEPLGIDYPFWETDQNGIEAGGWGIYLKTEDIAKIML
                     CYAQGGKFEGKQIIPEEWTKVATMKHADNSLGTSKDASSGYGYCFWICGTDTPAYRCE
                     GMFSQFGIVFKDYDAVLVFTGAEPMTQKTLDCIWRHFPAAFDNAGKEGFVAENFSEAI
                     SNLVLDRPSAGDRSGMEKRLEGKVIKMRKKLLLNLVGYPMSVLPLAVTYMTTDKAGNI
                     DNIIFNFGDDVCQFSWSEGDERNKIPCGMDGKYREGLMYLGGIKYKVLAAAKWKSDNE
                     LILDIRPVETIAKRRLFFTFKDKNKVVMTPASTPDVKNITNFIKDGAKDIFSNKFVVM
                     LAQKIIDYLPAIVEPVHKGKVTNKTVEDLHREAHS"
     gene            complement(4888..5037)
                     /locus_tag="PLD98_07795"
     CDS             complement(4888..5037)
                     /locus_tag="PLD98_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06181.1"
                     /translation="MSRIAWVAKMAAKIAKIFWKLSNIPFFQFLISYFGPAGGDAEED
                     TTAAD"
BASE COUNT         1340 a         1242 c         1281 g         1289 t
ORIGIN      
        1 ggatttgagc atggcctttt gcctgtcgcc cgagaggttt acgggtttgc ccgaatagtc
       61 ggcgatgccg tggaggataa tcggagccgc gggcaccggc tcataggctg cgctctcctt
      121 gtagtaggtg gaaacgggca ggttaactat gatatcagcg ttctttacaa gatagaagtt
      181 gcccgtatcg accattagac cccgctgtgt cgcaagggcc tgaatgtggg aggcgaccag
      241 cttttttgcc tcctgacggt ttatgtagcc gccgccgaag tcggaataaa ggagcttgcc
      301 ggcgtccttt atggaaaagc ccgtaaagga tgtatccttt aaattggtca ggaggtccac
      361 tatcttgacc tccagctttt cgagcttcaa gccttcggta tagaaagtct tagccccgac
      421 atggggatag aggggattct ggttttcgat tgtcagcaca tctcctgagg aagtgcgggc
      481 gcagaaggag gagagatagc ctttaagtcc tcccgaggtg atgacattta catcaatgta
      541 aagacccgac ttttgtcttt caagatagga gctgagtctc tcgagagcgg attttccgcc
      601 cagagtcttg agcaccgatg cctttgcgga gtcgtgctga acaatgccgc cggacaaaag
      661 tccagaatag cgcatattga tgctgtctat gcccttgccc tttaagattt cggcaaggtc
      721 ctgagcctgg tctatcttag taaggctttg ataaaagccg cttttcttct tgcctgcgcc
      781 tataacgctt aggttaaagg ggaacttgtc ggtgtaattt accttctttg tggagagcac
      841 gccctctctt ataagctgct cccttgccgc tcttgccatg ccgctgtaat tcgcgctctt
      901 gccagagata aagcggtagc agattttgat attaccgtcg tactgctcgg atatgtagga
      961 ctttttgtcc agcttgttct gggagcgaag cacgaaggat gcggataccc ttgcggggga
     1021 gagagcggaa gctctgcgag cctgaatttc cgccaaggcg tcgccgtcct caattatagc
     1081 ggcaaaggcg ttatccgagt ccttaacgcc gaaaacgggc attatgaccg ttggtgaggt
     1141 gtctgccgac acgctgtaaa gggaggccgg gtcgggcccg tagacgggga gatttacaaa
     1201 atttgtgctg tcgtccacct tgccggtaaa cataagcgct ccggggccgt cgggcaagag
     1261 gaaataatcc tcacccgcgc cgccgaaggt gctgccgagg tacggcaggg gatttaatcg
     1321 ctcaatatac agcccgtcgg aagcggtaac cttagagcag tccgcggtga aataaaagtt
     1381 gccgtcggaa agggtaaact caacctcagc cttaattacg gcgtccttgt ttcctttaag
     1441 gaagattttg gccgtatttt cgtcaaggct gaaaacataa tttatcttaa ctccgttttc
     1501 cgtctgctca aagccggctc cccccatggc gacgctgttg tcctgggagt ttaaatacaa
     1561 aatgccgttt ttggtaagca cattaaggct taagacggag gcgtatgtat tgttctctac
     1621 gggcatagaa agccacatct tgccgcccgt cgtctcctta acggcaaccg ccagagagac
     1681 ctcgtcaata taaagctcca aaagacccga tgaggcaacc tttttaagtg aacccaagtc
     1741 aatggtgtgg tacctcgggt ccgcggcttt ggggctgagc acagtgcccg tatccgcctt
     1801 aatggcggtg gcgcccccgc cgacggagca tgcgggcagc agagtcatca atattaaaat
     1861 tatcgaaagc aaaacgcatt tgattttcat ttcattctgt cctttcgggg tggaggaaag
     1921 gctaaaatta ttcactatta tgatagcaca ttgatttttc cctatcaagc aaagcggcaa
     1981 tataatgcca aggatagttc tggtatggaa aatctgcctg taaacctcca accctatggg
     2041 gttacggcga ggatatgtat tctctgagtt tctcgaaatc ctcgggaacg ggtacgcaga
     2101 aggtaaggct cgcccttgtt accggatgta taaacgaggc tttggaacag tgcagggcgg
     2161 ggcgggagat aagctctgtg ctgccgccgt acatatcgtc gcccgcaagg ggcgcgccca
     2221 tggaggcaaa atgcaccctt atctgatgcg tcctgcccgt ttccagcctg attcttaaaa
     2281 gggaatggcc cttgcccgtc aaaacagact cccagtgagt tacagcggga acggcgtcct
     2341 ttctttctcc agcggctcta agggttttca tcgggtcggg tttgtatatg ggtttgtcta
     2401 tggtgccgct gccctcaaaa atgccctcgg caatgcaaag gtactccttt tccacatttc
     2461 ccgaaagggc ggcggccgca aagcggttaa gagcgcatat aaccagcccc gaagtgcctc
     2521 tgtctagtct gcctacagcc ctgaacgcgg cggcctttcc cttgcttaaa agatacgccg
     2581 ccacagagtt tgccagcgta ccctccaggt gattgtgact cgggtgcatg ggcattaaag
     2641 cgggcttatt tataaccagt atgtctgagt cctcatatat aatctcgggc atctcgcctg
     2701 aggggataac ggttggttct tcgtcgggaa tggacaaagt cagtacatct ccctttttca
     2761 ttatgtcgat ggtgcgggca tggcagccgt ttaagaggat gccgttttct atccgcttta
     2821 aagaagtaac aagacgggcg gacatcttaa gatgtcccct gagaaagctt tgaatctttt
     2881 taccgtcaaa ttcgtcgccg atcaggaact ctaactgtct tgacatttca cgcctcgcag
     2941 tgtttattaa atcattttaa ccgcatagag ataatagtac aagccaaaaa atattatttt
     3001 tttttgaatt actatgttag cattcggaaa ttattgcaga tatgtgcaaa ctttatatcc
     3061 aaatttttca gataattaga attttgttca tacaatttaa atatgagggt actacaatat
     3121 caaaatgtag ttgaatttgt ctttcaggag ttcctttaag gttttcaagg aggttcttat
     3181 gtctaatgta ataattccgc gagccgagag cccggaagcg gttggagttt cctccaagtg
     3241 catattggat ttgattgagg atttcaaaaa cagcgatatt gaaatgcact cccttatggt
     3301 tataaggcac ggcaaggttg ccgccgagtg ctacaattac cccttctcag cggaaagacc
     3361 ccacgcatgc tactcaatat caaagagcat tacctcaacc gcaatcggct ttgcggtaag
     3421 cgaaggcatt ttaagccttg acacccgcct tatcgacatc ttccccgagt acaaggaaaa
     3481 ggcggacgag aatttaaagc agctcaccat tgagcacctt gtttcaatgc gttcgggcaa
     3541 gaatacggat attcttaaaa acaaggcaaa gattgactgg atagacgact atatgaaatc
     3601 cccgtggtac gcaaagcccg gcaccgattt cagatatgta aacgagaaca tttatatgct
     3661 cagcgcgtct ttgcacaggc tcacgggcat gaacatgagg gactttttaa gaccccgcct
     3721 ctttgagccc ctgggcattg actatccctt ctgggagacc gaccaaaacg gcatagaggc
     3781 aggcggctgg ggcatctacc taaagacaga ggacatagca aagattatgc tctgctacgc
     3841 tcagggcgga aaattcgagg gcaagcagat tattcccgag gagtggacga aagtcgccac
     3901 catgaagcac gccgacaaca gccttggcac cagcaaggac gccagctccg gctacggcta
     3961 ctgcttctgg atttgcggca ccgacactcc cgcataccgc tgcgagggca tgttctccca
     4021 gttcggcatt gtatttaagg attacgacgc cgttcttgta ttcacgggcg cggagcccat
     4081 gacccaaaag acccttgact gcatctggcg ccacttcccc gctgcctttg acaacgccgg
     4141 caaagagggc tttgtcgctg agaacttcag tgaggctata tcaaatctgg ttctcgacag
     4201 accctccgcg ggcgacagaa gcggcatgga aaagaggctc gaaggcaagg tcataaagat
     4261 gcgcaaaaag ctccttctga atttagtcgg ctacccgatg agcgttctgc cccttgccgt
     4321 tacatacatg acaaccgaca aagcgggcaa tattgataat attatattta acttcggcga
     4381 cgatgtatgc cagttctcat ggtcagaggg cgacgaaagg aacaaaatcc cctgcggaat
     4441 ggacggcaaa tacagggaag gtcttatgta cctcggcggt atcaaataca aggtactcgc
     4501 cgccgccaaa tggaagagcg acaacgagct cattttggac ataagacccg ttgaaaccat
     4561 agcaaagaga aggctgttct ttacctttaa agataagaac aaggtcgtaa tgacacccgc
     4621 cagcaccccc gatgtaaaga acatcacaaa ctttataaaa gacggggcga aggatatttt
     4681 ctcaaacaag ttcgttgtaa tgctggctca aaagattata gattacctgc ccgccatagt
     4741 cgaacccgtt cataagggca aggttacaaa taaaacagtt gaggacctgc accgcgaagc
     4801 gcactcttag ctgaaaaaga gtaaaaatta aaatggcaac gatagctcgt tgccatttta
     4861 attatgcgcg cacactaaac tcaaaaatta atcagctgct gtggtatctt cctcagcatc
     4921 gccgccggcg gggccaaagt aagaaataag gaactggaag aaaggaatgt tggagagttt
     4981 ccagaatatc tttgctattt tagccgccat ttttgcaacc catgctattc gactcatcaa
     5041 aaagcctcct ttcagccaca attgcaaatt cgcactatgg tatgtaccaa ttataaacaa
     5101 aaaaaatgta ttaatcaaga ctttttgtgt cacctcagtc tttaattcac aa
//
LOCUS       DAONVQ010000129         5041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_12063_length_5041_cov_4.128159, whole genome shotgun sequence.
ACCESSION   DAONVQ010000129 DAONVQ010000000
VERSION     DAONVQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12063_length_5041_cov_4.128159"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /gene="aroC"
                     /locus_tag="PLD98_07800"
     CDS             complement(<1..993)
                     /gene="aroC"
                     /locus_tag="PLD98_07800"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQA06182.1"
                     /translation="MNSEFGERIKVSIFGQSHSQAIGAVITGLPAGLKLDTDYIDSFM
                     KRRAPGRNIYSTQRKEPDKPSVVSGLAGGYTTGDPLCVVIKNTNTISSDYTGFKDTPR
                     PSHSDYPLFVKTGGYNDIRGGGHSSGRLTAPLCFAGAIALQILKKFGIEVGAHIYSIG
                     GAEDTPFDMTDISPELLAKVKEKDFPTISDDAGKLMRVEIEKARLELDSIGGIIECAA
                     VNLPAGLGSPMFDGVENKIAAAVFGIPAVRGIEFGAGFKASKMRGSEHNDPYFIKDGK
                     IATKTNNHGGAIGGITTGMPLVFRAAIKPTPSISKEQDTVSISEMENKKLAIKGR"
     gene            complement(1008..2291)
                     /gene="aroA"
                     /locus_tag="PLD98_07805"
     CDS             complement(1008..2291)
                     /gene="aroA"
                     /locus_tag="PLD98_07805"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HQA06183.1"
                     /translation="MDIKIIPRALTGKVKAISSKSDAHRLFFAAAAAEEETSIVINGK
                     AGEDILATINCLKALGAEIHERKTDADETEYTVIPAGRVESDILPTLDCGESGSTARM
                     LLPLATYISPKGFKMTGRGRLPERPMTPLIDELIKKGCEFTSKSIPLEVKNTPAGGVF
                     KIAGNISSQFISGLLFLLPLLPDGGIIEIDPPLQSAGYVDMTVKTLSLFGVKVERNDN
                     RITVKGGQRYISPQKAYAEGDWSSAAFWLCAAALNGSITVTGLDAESAQKDKYVFDIL
                     SRMGAKCSQTDGEFTISANSLKGTEIDASEIPDLVPVLSVVCAAAEGESVIFNAGRLR
                     LKESDRIESTCTMLKSLGAEVTSDNDSIRIIGKKTLSGGTVDSFNDHRIAMSAAVASV
                     ICENPVIILGAECCAKSYLSFFDDFNKLGGKTEIL"
     gene            complement(2307..3044)
                     /locus_tag="PLD98_07810"
     CDS             complement(2307..3044)
                     /locus_tag="PLD98_07810"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HQA06184.1"
                     /translation="MGDLTGFAASCYLRGIEFVQIPTTLLAAVDSSVGGKTAVNLKAG
                     KNLAGAFWQPSLVICDPETFNTLSPSELANGISESVKYGIICDRQLYEDIETNGFTEI
                     EKKIERCVSIKRDIVESDEFDKGKRKLLNLGHTFGHAIEKCSNFEIQHGAAVAIGMTA
                     AAKVSEKLNLSEEKPHDRIKSLLEKLNLPTECPYSYEELYEAALSDKKVESDKISLIL
                     IRKIGDSFIYNVSIKELKDIFYIAFGG"
     gene            complement(2956..3366)
                     /locus_tag="PLD98_07815"
     CDS             complement(2956..3366)
                     /locus_tag="PLD98_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06185.1"
                     /translation="MAERVVRVNASKSYDVIISKGSINKAGEYIKKVFPSLKNAVIVS
                     DDNVFPLYGEAVKKTLNENEIRALEFVVPHGEKSKDITVLSQLLEFLAENKVDKKSVL
                     LARGSYRRPYGLCGIVLSQRHRVCSNSHHPPCRG"
     gene            complement(3380..4237)
                     /locus_tag="PLD98_07820"
     CDS             complement(3380..4237)
                     /locus_tag="PLD98_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HQA06186.1"
                     /translation="MKIGIVGLGLIGGSFAKAIKARTEHTVLGADIDKEILSKAMDDG
                     AVDFELKDGDLKDCDLLLIALYPAAIISYLEEKARLFSKKGFVIDLCGVKRHICKKGQ
                     MLAKEHGFNFIGGHPMAGREVFGYSAAIENLFDGASMILVPEPERTVPDFIKEFFLSV
                     GFTHIQISTPEHHDEVIACTSQLAHVVSSAYVKTPVAPDFYGFSAGSFKDMTRVAYMN
                     EKVWTELFLNNADMLKLEIDSLVNELKKYSDALERRDEKEIMRLIKEGTEIKVRLNRL
                     EEMRKTGRL"
     gene            complement(4252..>5041)
                     /gene="aroF"
                     /locus_tag="PLD98_07825"
     CDS             complement(4252..>5041)
                     /gene="aroF"
                     /locus_tag="PLD98_07825"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQA06187.1"
                     /translation="DTVIEVGGTKIGGGNLGLIAGPCSVESTEQIVEIAKKVKAAGAN
                     FLRGGAYKPRSSPYSFQGLRDKGIKMLLEAKKETGLPIVTEIMDISQLEHFEEIDVIQ
                     VGTRNMQNFELLKELGKIRKPIVLKRGMSATFEELLMSAEYISAGGNTQIILCERGIR
                     TFEPATRNTLDISAVPLLKKLSHLPVIVDPSHATGKSSLVKSMSLAAAAAGADGIMIE
                     VHNDPPNAWSDGQQSITPDEFKDTVDAVKKIWSFCYGVVNEKKD"
BASE COUNT         1088 a         1297 c         1233 g         1423 t
ORIGIN      
        1 cctgccttta attgccagct ttttattctc catctcgctg attgaaacgg tgtcctgctc
       61 ctttgagatt gacggcgtgg gctttatcgc cgctctgaaa acaaggggca tgcccgttgt
      121 gatgccgccg attgcgccgc cgtggttgtt tgtcttggtg gcgattttgc cgtcctttat
      181 aaaatagggg tcgttgtgct cgctgccccg catttttgag gctttaaatc ccgctccgaa
      241 ttcgattccc ctgactgcgg ggattccgaa aacggctgcg gcgattttgt tttcaactcc
      301 gtcgaacatc gggctgccga gacccgccgg gaggtttacc gcggcgcact ctataattcc
      361 gccgattgag tcaagctcca gccttgcttt ttcaatctcc acgcgcataa gtttacctgc
      421 gtcgtcgcta atggtgggga aatccttttc tttgactttc gcaagaagct caggcgatat
      481 atccgtcatg tcaaagggtg tgtcctcagc gccgcctatg gaataaatgt gcgcgccaac
      541 ttcgatgccg aattttttta atatctgcag ggcgatggct cccgcaaagc acaagggggc
      601 tgtgagcctg cccgaggagt gcccgcctcc tcttatgtcg ttatagccgc ccgtttttac
      661 aaacaggggg taatccgaat gggagggtct tggggtatcc ttaaaccctg tgtagtcgga
      721 gcttatggtg ttcgtgtttt taatgactac gcacaagggg tcgcccgttg tatagccgcc
      781 cgcaagaccg ctgacaacgc tcggcttgtc gggctctttt ctttgtgtgg agtaaatatt
      841 tctgcccggc gcccgccttt tcataaagct gtcgatatag tccgtatcca gctttagtcc
      901 cgccggcaga ccggtaatca cagcgcctat cgcctgagag tgtgactgcc cgaatatgct
      961 gacctttatt ctttcaccga attcggaatt catatttacc tccgaaatta aagaatttct
     1021 gtttttccgc cgagcttatt aaagtcgtca aagaaggaaa gatatgattt tgcgcagcac
     1081 tccgcgccga ggataattac ggggttttcg caaataaccg acgctaccgc cgccgacatg
     1141 gcgattctgt ggtcgttaaa actgtccaca gttccgccgc tgagagtctt tttgccgatt
     1201 atgcgtatgc tgtcattgtc ggaagtgacc tcagctccga gacttttcag catcgtgcaa
     1261 gtgctttcga ttctgtcgct ctctttaagt cttagcctgc ccgcgttgaa gataacgctt
     1321 tcgccctcag cggcggcgca gactacggac aaaactggca caaggtcggg gatttccgag
     1381 gcgtcaattt ccgtaccttt aaggctattc gcgcttatag taaactcccc gtctgtttgg
     1441 ctgcactttg ctcccatgcg gcttaatatg tcaaagacat atttatcctt ttgagccgat
     1501 tctgcgtcaa gacccgttac ggtgatactg ccgtttaacg cggcggcgca aagccagaac
     1561 gcggccgatg accagtcgcc ctccgcatag gctttttggg gagagatata acgctgaccg
     1621 ccttttacgg tgatacggtt atcgtttctt tcaaccttaa cgccgaaaag ggaaagggtt
     1681 ttgacggtca tatccacata gcccgctgac tgaaggggcg ggtcaatttc tataatgccg
     1741 ccgtcaggca gaaggggcag gagaaacaaa agacccgaaa taaactgtga gcttatattg
     1801 cccgcaattt tgaaaacgcc gcccgccggg gtgtttttga cctctaaggg gatggatttt
     1861 gaagtaaact cgcagccctt ttttataagc tcatctataa gcggggtcat gggacgctcg
     1921 gggagtctgc ctctgcccgt catcttaaag cccttgggag aaatgtaagt ggcaaggggc
     1981 aacagcatcc tcgcggtgga gccgctctcg ccgcagtcaa gggtagggag aatatcgctt
     2041 tcgactctgc ctgcgggaat gacggtgtac tcggtttcat ccgcatccgt tttgcgctcg
     2101 tgtatttcag cccccaaggc tttaaggcag tttatcgtgg ctaaaatatc ctcgccagcc
     2161 ttgccgttta taacaatgct agtttcttct tccgccgccg cagccgcaaa gaacaggcgg
     2221 tgggcgtcgg actttgagga tattgcctta accttgcccg taagagcgcg gggaatgatt
     2281 tttatatcca tcttaacctg cctttctcag ccgccaaagg caatataaaa aatatctttc
     2341 agttctttaa ttgaaacatt gtaaataaag ctgtcgccga tttttcttat aagtataagg
     2401 ctgattttat ccgactccac ttttttgtcg gaaagagccg cctcgtaaag ctcctcataa
     2461 gaataggggc attctgtggg gaggttcagc ttttcgagaa gcgatttgat tctgtcatgc
     2521 ggcttttctt cagagaggtt tagtttctct gaaacctttg ccgcggcggt cattccgatt
     2581 gccaccgccg cgccgtgctg gatttcaaag tttgagcact tttctattgc gtgaccgaag
     2641 gtgtgaccga gatttaaaag ctttcttttt cccttgtcaa actcgtcgga ctccacaatg
     2701 tcacgcttaa tcgaaacgca gcgctctatt ttcttttcta tctcggtaaa gccgtttgtt
     2761 tcaatatcct cataaagctg tctgtcgcag ataataccgt attttacgct ctcgctgatt
     2821 ccgtttgcca gctccgaggg gctcaaggta ttaaaggttt ccgggtcgca gattacaagg
     2881 gagggctgcc agaaggctcc cgcaaggttt tttcccgctt tgagatttac agctgtcttg
     2941 ccgcccacgg acgagtcaac cgcggcaagg agggtggtgg gaatttgaac aaactcgatg
     3001 cctctgagat agcacgatgc cgcaaagccc gtaaggtcgc ctataactcc cccgcgccaa
     3061 aagtacgctt ttcttgtcca ctttgttctc ggcaaggaat tcgaggagct gagaaagaac
     3121 cgttatatcc ttgcttttct caccgtgggg aaccacaaat tcgagagcgc ggatttcgtt
     3181 ttcgtttagt gtcttcttaa ccgcctcccc gtataggggg aatacattgt cgtcggaaac
     3241 aattaccgca ttcttaaggc tcgggaatac cttttttata tactcccccg ccttatttat
     3301 actgcccttt gagattatta catcatagct ttttgaagcg tttactctga ctactctttc
     3361 tgccaaatct ttctcccctt taaagccttc ccgtctttcg catttcttca agccgattca
     3421 gtcgaacttt tatttccgtg ccctctttta taagccgcat gatttccttt tcgtcccgcc
     3481 tttcaagggc gtcggagtat tttttaagct cgtttacaag gctgtcaatc tccagtttca
     3541 gcatatcggc attgtttaaa aaaagctctg tccagacctt ctcgttcatg taggcgaccc
     3601 ttgtcatgtc cttaaagctg cccgcggaaa aaccgtaaaa atcaggagca acgggggtct
     3661 ttacataggc gcttgacacc acatgggcaa gctgtgatgt gcaggcgatt acctcgtcat
     3721 ggtgctcagg agttgaaatc tgaatatggg taaaaccaac tgacaggaaa aactccttaa
     3781 taaagtcggg aacagtccgc tcgggctcgg gcacaagtat catggaggct ccgtcaaaaa
     3841 ggttctctat tgcggctgag tagccgaaaa cctccctgcc cgccatggga tgaccgccga
     3901 taaagttaaa gccgtgctct ttagcgagca tttgcccttt tttacaaata tgccttttta
     3961 ctccgcaaag gtcaataaca aatccttttt tgctgaaaag gcgcgccttt tcctcaaggt
     4021 aggaaatgat ggccgcgggg taaagggcta taaggagcaa atcgcagtcc tttaagtcgc
     4081 cgtcttttag ctcaaagtcc accgccccgt catccatagc ctttgacagg atttctttat
     4141 caatatccgc tccgagaacg gtatgctcgg tgcgggcttt tatcgctttg gcaaaggagc
     4201 ctccgataag ccccagcccg actattccta tcttcaaaat atcacctcgg gtcagtcttt
     4261 cttttcgttt acaactccgt agcagaagct ccagattttc ttgaccgcgt cgaccgtatc
     4321 tttaaactca tcgggagtga tggactgctg tccgtccgac catgcgttcg gcgggtcgtt
     4381 gtggacctct atcataattc cgtcggcgcc cgctgcggct gccgcaagtg acatggactt
     4441 tacaagtgag gacttgcccg ttgcgtggct ggggtcgact atgacaggca ggtgggacag
     4501 ttttttgaga aggggcacgg cggagatgtc aagggtgttt cttgttgccg gctcaaaggt
     4561 tctaatgcct ctttcgcaga ggataatctg ggtattgccg ccggcggaga tgtattcagc
     4621 gctcatgagc agttcctcaa aggttgcgga catacctctt ttaaggacta tgggctttct
     4681 gattttgccc agctctttca gaagctcgaa gttctgcata tttctcgtgc cgacctgaat
     4741 tacatctatt tcctcaaagt gctcaagctg tgagatgtcc ataatctctg ttacaatggg
     4801 cagacccgtc tccttctttg cttcaagcag catcttaatg cccttgtcac ggagaccctg
     4861 gaaggagtag ggtgaggacc tgggcttgta tgctccgcct ctgaggaagt tggcgcccgc
     4921 ggcctttact ttctttgcaa tctccacaat ctgctcggtg gactcaacgg agcagggacc
     4981 tgcgataaga cctaagtttc cgccgccgat tttggtgccg cccacctcta tgacagtgtc
     5041 g
//
LOCUS       DAONVQ010000130         4951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_12333_length_4951_cov_4.784722, whole genome shotgun sequence.
ACCESSION   DAONVQ010000130 DAONVQ010000000
VERSION     DAONVQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12333_length_4951_cov_4.784722"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..2339
                     /locus_tag="PLD98_07830"
     CDS             132..2339
                     /locus_tag="PLD98_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HQA06188.1"
                     /translation="MYSQDYKEKISGIVDRVVFRNEENSFTVIELNCGGELVTAVGSL
                     PELSSGEEISLYGTWDSHTLFGRQFKASQIERKMPETASQLLRYLSAGTIKGIGAKTA
                     LKIIEQFGENSFKVLSEEPERLTQIRGISLEKARRISDEYNSQMAIRTVMIALEGYGL
                     KAHECIEIFKILGPSAVAAVENNPYILCSILSGFTFERAEEIAAKLPEKPDNKHRLRA
                     GIEFILRHNLGNGHTCVPYDKIVSLSATYLNEGTDAIEIAIDEMLENQLLYSAEIDGR
                     LFIFLPEMYRAERKAAERLLALMKFSPGSVGALREEIESIETAVGISYGELQKKALET
                     AAMKGVMILTGGPGTGKTTTLKGIISLFKKRGLHIALAAPTGRAAKRMTEVTGMEAKT
                     IHRLLEVEWGDGERPVFRRDLQNPIDADAIIVDELSMVDISLFSNLLEAVPFGCRLIL
                     VGDSDQLPPVGAGNVLHDLIDSGLIPVIKLNQVFRQAEKSRIVTNAHRIIRGEMPEIS
                     NEGDFFFLERKSADLAARTVRELCCERLPDAYGISPIEDLQVLCPSRNGVCGTANLNR
                     LLQERLNPRDLSKAEIKSGGFVFREGDKVMQTKNNYNLDWTSGFSEGTGVFNGDLGVI
                     KKIKPAEGVLVIDFDGKEVLYPAEKLSDLELAYAITVHKSQGSEYDTVILPIIDSSAK
                     LLYRNLLYTAVTRAKRMIVIVGLKSKLEAMVNNDKQSKRYSALKSFIENGVLI"
     gene            complement(2365..3618)
                     /locus_tag="PLD98_07835"
     CDS             complement(2365..3618)
                     /locus_tag="PLD98_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HQA06189.1"
                     /translation="MKFGLIGKKLAHSYSKIIHELMGYPYDLIELEESELKEFFAAKD
                     FDAINVTIPYKTDVMPYLHEISEKAQKIGSVNTIVKKSGGRLVGYNTDYDGFVYMLNR
                     AGISVTGKKVLILGSGGTSKAALAVCNDLKAKEIIIISRSGENNYENIERHADADVII
                     NTTPVGMYPHNGKSPVSLDIFTKLQGVVDVIYNPLKTELLLQAEEKGIKFTNGLPMLC
                     AQAVYAADHFFEVCPEIRPKLTFSEKEPLEESIIKEIEKLCTNIIFIGMPGCGKSSIG
                     KAVAEITGREFYDTDAIVEEITGMSNQDFLHKYGEAAFREKEAKIAEEVGKKSGAVIS
                     TGGGIVKNPQNVKHLRQNGKLIFIDREIEKLSLVGRPLSKDLATLKQMYIERLPLYKA
                     AADMILYNVGTVKSAARAIVEMLEY"
     gene            complement(3631..4773)
                     /locus_tag="PLD98_07840"
     CDS             complement(3631..4773)
                     /locus_tag="PLD98_07840"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HQA06190.1"
                     /translation="MSLSEIRERIDKIDDQLTALFVERMNLAAEVAEEKKKSGLPVYS
                     PKREREILSKVTSQMPDNIAIYAKSFFTTLFDVSSSYQSRLIGTKSTLKEQIKKAVEE
                     GPKELPKKAIVACQGVEGSYSQQVCDRLFALPSIMYFTSFESVFRAVEQNLCQYGILP
                     IENSIHGSVTQVYDLMHKYNFHIVKSVKQKIHHTLLVNKGAKLEDIKEIYSHEQAIGQ
                     CSDFIASLKDVKVNYSANTAMAAKYVAESGRTDIAAISSENCADIYGLEVLSSDIQNS
                     NSNYTRFICISKDLQVLPGADKISLMATTAHRPGSLYRLISKFAVMGINLTKLESRPI
                     PGKDFEFKFYFDMEASVYNDDVLDLLGELSNAPETFSFLGAYSEMS"
     gene            complement(4787..>4951)
                     /locus_tag="PLD98_07845"
     CDS             complement(4787..>4951)
                     /locus_tag="PLD98_07845"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQA06191.1"
                     /translation="DMQGQAQEVITKGRHDPCIVPRAVPVFEAVTALAILDLMINEYG
                     CRSISDKVRE"
BASE COUNT         1237 a         1144 c         1223 g         1347 t
ORIGIN      
        1 cgctggcgag ccccagtact gcgtagcaag gggtataggc gccgctctca gaaatgccga
       61 tattctcaga accagcagtt ttgctttaag tcaggacgag cttttggcag cgtcaaacat
      121 ttagggtgtt tatgtatagc caggactata aagaaaaaat cagcggcata gtggacaggg
      181 ttgttttccg taatgaggaa aacagcttta ctgttattga gctaaactgc ggcggtgagc
      241 ttgtaaccgc cgtcggttcc ctgcccgagc tttcttcagg cgaggaaatc agcctttacg
      301 gcacctggga cagccatacc cttttcggaa ggcagtttaa ggcgagtcaa atcgaaagga
      361 agatgcccga aaccgcctct cagcttttac ggtatctttc ggcgggcaca atcaagggca
      421 tcggagccaa aaccgcactt aaaataattg agcagttcgg agaaaacagc tttaaggtac
      481 tctcagaaga gcctgagagg ctgactcaga taaggggcat ttcccttgag aaggccagaa
      541 gaatttcgga cgaatacaac agccagatgg caatcagaac ggttatgatt gcccttgaag
      601 gctacgggct taaagctcat gagtgcatag agattttcaa gattctcggc ccctccgctg
      661 tggcggcggt ggaaaataat ccatatatat tatgctctat actcagcggt tttacttttg
      721 aaagagcgga agaaatagca gcgaaactgc ccgaaaagcc cgacaacaag cacaggctca
      781 gagcgggtat tgagtttata ctgaggcata atttgggcaa cgggcataca tgcgtgccct
      841 atgataaaat agtttccctt tccgccactt accttaatga gggaaccgac gctatagaaa
      901 tcgccataga cgaaatgctg gaaaatcagc ttctctactc cgccgagatt gacggcagac
      961 tctttatatt cctccctgaa atgtataggg cggaaaggaa ggcggcggag cgtctgttgg
     1021 ctttaatgaa attctccccc ggctccgtgg gcgctttgcg tgaggaaata gaaagcattg
     1081 aaaccgccgt cggcatcagc tacggggagc tgcagaaaaa ggctcttgaa accgcggcga
     1141 tgaaaggcgt tatgattctg acgggcggac ccggaacggg aaagaccacg acgctgaaag
     1201 gcattatcag cctatttaaa aagaggggtc ttcacatagc ccttgccgct cccacgggaa
     1261 gggcggcaaa gcgcatgacc gaggttacgg gcatggaggc aaagaccatt cacaggctcc
     1321 ttgaagtgga atggggcgac ggggaaagac cggttttcag aagggatttg cagaacccca
     1381 tagacgccga cgcgattata gtcgacgagc tttcaatggt tgacattagc ctcttttcaa
     1441 atcttttgga ggcggttccc tttggctgca ggctcatctt ggtgggtgac tccgaccagc
     1501 tcccgccggt cggggcggga aatgtcctgc acgacctaat agattccggg ctgatacccg
     1561 ttataaagct caatcaagtc ttcaggcagg cggagaaaag ccgcattgtc accaacgccc
     1621 acagaattat tagaggagag atgcccgaaa taagcaatga gggagatttt ttcttccttg
     1681 aaagaaaaag cgccgacctt gcggcaagaa cggtaaggga gctttgctgc gaaagactgc
     1741 cggatgcgta cggcatttct cccatagagg atttgcaggt tctctgcccg tcaagaaacg
     1801 gggtctgcgg cacggcgaac ctaaacagac tccttcagga aaggctgaat ccgagggatt
     1861 taagcaaagc cgaaataaaa tccggcggct ttgtgttccg cgagggcgac aaggttatgc
     1921 agacgaaaaa caactataac cttgattgga caagcggatt ctccgagggt acgggcgtgt
     1981 tcaacggcga cctgggcgtt attaagaaaa taaaacccgc cgaaggggtt ttggtaatcg
     2041 actttgacgg caaagaggtt ttatatcctg ccgaaaagct ttccgacctt gagcttgcct
     2101 atgcgataac cgtacacaaa agtcagggca gcgagtacga tacggtcatt ctgcccatta
     2161 tagattcatc ggcaaagctt ttataccgca atcttttata taccgcggtg acaagggcga
     2221 aaagaatgat tgtgatagtg ggactgaaat ctaaacttga ggctatggtc aacaacgaca
     2281 agcagagcaa aagatattcg gctctgaaat ctttcattga aaacggagtt ttaatataaa
     2341 aagccgctga gggttcagcg gcttttaata ttccagcatt tcaactattg ctcttgctgc
     2401 ggacttaaca gtgcccacat tgtaaagaat catgtctgcc gcggctttgt aaagcggcag
     2461 tcgctcaata tacatctgct ttaaggttgc caggtccttt gaaagcggtc tgccgacgag
     2521 actaagtttt tcaatttccc tgtcaatgaa aatcagcttt ccgttttgcc ttaaatgctt
     2581 tacattttga ggattcttaa ctatgccgcc gccggttgag ataacggcgc cgctcttttt
     2641 gcccacttcc tcagcgattt tcgcctcctt ttctctgaag gctgcctcgc cgtatttgtg
     2701 taagaagtcc tgattgctca tgccggtgat ttcctcaact atggcgtcgg tatcgtaaaa
     2761 ctccctgccc gttatctccg caaccgcttt gccaatgctg cttttgccgc agccgggcat
     2821 tccgataaat atgatgtttg tgcaaagctt ttcaatctct ttaataatac tttcctccaa
     2881 tggttccttt tcagaaaagg ttagtttcgg gcggatttcg gggcatacct caaagaagtg
     2941 gtccgcggcg taaaccgcct gagcgcaaag catgggcaaa ccgtttgtga actttattcc
     3001 cttttcttct gcctgcagta aaagctcggt tttaaggggg ttgtagatta catcaaccac
     3061 gccttggagc tttgtgaaaa tgtcgagact aacggggctt tttccgttgt gggggtacat
     3121 gcccacgggg gttgtgttga ttatcacatc ggcgtcggcg tgtctttcta tattctcata
     3181 attattctcg ccgcttctgg atattataat gatttcttta gcttttaagt cattacacac
     3241 tgccaaagcg gcttttgagg tgccgccgct gccgaggata agcacctttt tgcccgtgac
     3301 ggagattccg gctctgttca gcatatacac aaagccgtca tagtccgtat tgtagcctac
     3361 gagtctgccc ccgctttttt tgactatagt gtttacgctg ccgattttct gagctttttc
     3421 ggatatctca tggagatagg gcattacatc tgttttgtaa ggtatggtta cattaatcgc
     3481 atcaaaatct tttgccgcaa aaaactcctt aagctcagat tcttcaagct caatcaaatc
     3541 gtaggggtag cccataagct catgaattat tttcgaatag ctgtgggcaa gctttttgcc
     3601 gattagtccg aacttcatta gtatcaatcc ttatgacatt tcagagtacg caccgaggaa
     3661 ggagaaggtt tcgggagcgt ttgacagctc tcccaaaagg tcaagaacat cgtcattata
     3721 aaccgacgcc tccatatcga aatagaactt gaattcaaag tccttgccgg ggatggggcg
     3781 gctttcaagc tttgtaaggt ttatgcccat aacggcgaac tttgaaataa gcctgtaaag
     3841 cgaacccggt cggtgcgcgg ttgtagccat tagactaatt ttatcagcac cgggcagaac
     3901 ttgcaagtct tttgatatgc aaataaaacg ggtgtagttg ctgttgctgt tttgaatgtc
     3961 gctgctgagg acttcaagac cgtaaatatc agcgcagttt tcggaggaaa tcgcggctat
     4021 atcggtcctt cccgactcgg caacatattt tgcggccatc gctgtattgg ctgaatagtt
     4081 tacctttaca tcctttaagg atgctataaa gtcgctgcac tgaccgattg cctgctcgtg
     4141 ggagtaaatt tccttaatat cttcgagttt cgctcccttg tttacaagca gggtatgatg
     4201 aattttttgc tttacgctct ttactatatg gaaattgtac ttgtgcataa ggtcatagac
     4261 ctgagttacg gagccgtgga tgctgttttc tatggggaga attccgtact ggcagagatt
     4321 ttgctctacg gctctgaaaa cgctttcaaa gctggtgaag tacataattg agggcagggc
     4381 aaacagcctg tcgcaaacct gctgtgagta tgagccctca actccctggc aggcgactat
     4441 tgcctttttg ggcagctcct tgggaccttc ctcaacagcc tttttgattt gctcctttaa
     4501 ggtggacttt gtgccgatga gccttgactg ataggatgag cttacatcaa aaagggtggt
     4561 gaaaaagctt tttgcgtaga ttgctatatt gtcgggcatc tggcttgtga cttttgatag
     4621 tatttccctt tcacgcttgg ggctgtaaac ggggagcccg ctctttttct tctcctcggc
     4681 tacctctgcc gcgagattca ttctctccac gaaaagagcc gtcaattggt cgtcgatttt
     4741 gtctatcctc tctcttattt cactcaaact catttttacc ctctccttac tctcttactt
     4801 tgtcgcttat gcttctgcag ccgtattcat ttatcattaa atcaagaatt gccaatgctg
     4861 tgaccgcctc aaaaactggg acggctctgg ggactatgca ggggtcgtgc ctgcctttgg
     4921 ttatcacctc ttgcgcttgg ccttgcatat c
//
LOCUS       DAONVQ010000131         4906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_12458_length_4906_cov_4.974232, whole genome shotgun sequence.
ACCESSION   DAONVQ010000131 DAONVQ010000000
VERSION     DAONVQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12458_length_4906_cov_4.974232"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            518..982
                     /locus_tag="PLD98_07850"
     CDS             518..982
                     /locus_tag="PLD98_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HQA06192.1"
                     /translation="MVLDKLTNLKAYSKLIPKTDEILAAIEKVRAGEFTGSRLDIDGD
                     SIFLLNQEYETFDREGRQLEAHRKYIDLQAVIKGEEYIGWSELEGLTLASEEYSKGGD
                     IAFYNEEPKIYIPMTEGAFALFFPQDAHMPCIKMGDSRKVQKVVVKIAVDLA"
     gene            complement(1016..2278)
                     /locus_tag="PLD98_07855"
     CDS             complement(1016..2278)
                     /locus_tag="PLD98_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HQA06193.1"
                     /translation="MRDLTKCIHSGYEPKNTEPKNVPIVQSTTYVFDSTTEVGSVFDE
                     PTKALIYSRFANPTVMAVENKIAALEGGVAAMCTSSGQAATLIAVLNLCKAGDSIVST
                     SQIYGGTFNLFAVTLKKYGIDCVFVDCDADEAEIDAAIKDNTKLIFGETIANPALTVF
                     DFEKFSRVAKKHGIPLIIDNTFATPILCKPIEYGADIVIHSTSKYMDGHAVQVGGVIV
                     DSGNFDWTNGKFPDFTEPDESYHGIRYVENYGKAAYITKARMQLMRDFGAYPAANSAF
                     LLNLGLETLGLRMERYCENALKVAEYFAKSDKVERVSYPGLPSDKYYPLAKKYLKGAS
                     GVISFVIKGGKENAIKFMDGLKLASIEVHVADIRTSVLHPASSTHRQLTDEQLVAAGI
                     NPGLIRFSVGLEDIDDILEDIEQSLSKI"
     gene            2443..3018
                     /locus_tag="PLD98_07860"
     CDS             2443..3018
                     /locus_tag="PLD98_07860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQA06194.1"
                     /translation="MDNKPVITIKPYELKYRRDVQQLCLANANHLDKPFEDRRYILIM
                     YCNYYIEQEPENCFVAVNEEDKAIGYIICSESYKKYRERFNRYYIPQARSLGFSYYVK
                     AKLDMLSHAMFAKKYPAHLHIDIDDNYQRLGIGTRLMNQLVDHLKAKGVKPGLMLVVG
                     ANNEKGRNFYKKYGYKELAVTKMGVAMGIEL"
     gene            3049..4551
                     /locus_tag="PLD98_07865"
     CDS             3049..4551
                     /locus_tag="PLD98_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06195.1"
                     /translation="MDISSAVLFVVLAAVSMSYAWGMRGTIIGGEKGAMLPGAVMGML
                     IALFSSSEVLKDNFFMLSAVGAMSMYLGGCMTYGETLGLSMNSNPPENFKKSIIALVV
                     KGFIWFSLFGAFVGIALNSFAGLLYTVKDFILIFALIPVCALAGYFLLNKPLDPKNGK
                     FPFIYFSKTRQESWGGLLGILLVLVAVAAKNKDSFTLNLVLGAGLSGGIGWAIAQTLQ
                     AAARLPLIKGKRLFEKMNRKKTIDAWKLMEYSLGAIGGIGIALTLIAQHDKFSAIAAA
                     IDSRGAINLPLEKYSAVFTAVWLVLIALDQLQFIIKPRFSLSELKNQKIVKATLSESE
                     YEKCLKDAERKESSPHFKRYAFLVEKGEFALYSLIPMFFVLCGSSLVGRLTSFFILYL
                     VVVQEVVFGRFRLLKHRRLINTCLILGAVLILLYELALDTTFSMIATMIMYTIFYDIL
                     GILHVVPVEKDEKVKLGPFTMLNYCDLSVFGYMSVSGVVSVLLLALAIYF"
     gene            4589..>4906
                     /locus_tag="PLD98_07870"
     CDS             4589..>4906
                     /locus_tag="PLD98_07870"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HQA06196.1"
                     /translation="MRNTLRQIKIGNTEINGAAALAPMAGCSDLPFRELCRSFGASYT
                     VTEMVSAKGFVMGDRKSEELMRLGENEHPAAIQIFGSEPLYMAKAAEKALKFSPDIID
                     INMG"
BASE COUNT         1228 a         1241 c         1190 g         1247 t
ORIGIN      
        1 ttcgctaagg aaaaattcaa aatgctcctt gtagtgattt tccctcttaa cagatagttc
       61 tctattaata gcctttgaaa atcggccccg accccggggt cggctttctt tattgcgccc
      121 acgcccgccg gtgtcccaca ctgccgccga gggggtttcg cctaagccgc cgagggggtt
      181 ctgccagtac ctcacgcccc ttcaatggag tgctgcgtcc cgcgagggtg tgctgcccgc
      241 acctcccgcc gtagggtatg ctccccgccg ccctgtgctg cgcaccgtca aggagtgccg
      301 cccgaaccct gccgccgcgg ggtttcgcct cacccgccga agatgtgccg cacgcccccc
      361 ccgccccccg cacccctgcc gctgtgggtt tccgggccgc cgcccccgta ttgtcagggc
      421 gcgcggaaca aaaagcggca tgtttgccgc cgctattgcc aatgccgtaa aaaaggctta
      481 ttataattcc atagccgcat tttgaaagga gaaaaccatg gttttagata agcttacaaa
      541 tctcaaagca tattcaaagc tcatcccaaa gacggacgaa atccttgccg ccatcgaaaa
      601 agtccgcgcg ggggagttta cgggcagcag actggacatt gacggcgaca gcatcttcct
      661 tctgaatcag gagtatgaaa cttttgaccg agagggcaga cagcttgagg cccacagaaa
      721 gtacatcgac ctgcaggcgg taataaaagg cgaggaatat atcggctggt cagagcttga
      781 ggggctgacc ttagcctctg aggaatattc aaagggcggc gacatagcct tttataatga
      841 ggagccaaag atttatattc ccatgaccga aggcgcattc gccctgttct tccctcagga
      901 cgctcacatg ccctgcataa agatgggcga cagccgaaag gtgcaaaagg tagtagtaaa
      961 aatagccgtt gacttggcat aaaaaaaagg aatcccatcg ggattccttt aatttttata
     1021 tcttgctgag gctctgctca atgtcctcca gaatgtcgtc tatgtcctca agacctacgg
     1081 agaagcggat aagtcccggg tttattcccg ctgccacaag ctgctcatcg gtcagctgcc
     1141 tgtgagtgga gcttgcgggg tgcagcacgc ttgtgcgtat atccgcaaca tgaacctcaa
     1201 tggaggcaag tttgagcccg tccataaact taatggcgtt ctccttgccg ccctttatta
     1261 caaaggagat aactccgctt gctcccttga gatatttctt agccagcgga tagtatttat
     1321 cgctcggaag tccggggtag gacactctct caaccttatc ggactttgcg aaatactccg
     1381 caaccttcaa tgcgttctcg cagtagcgct ccattctgag acccaaagtc tcaagtccca
     1441 gattcaaaag gaatgctgag tttgcggcgg ggtacgcgcc aaagtcacgc ataagctgca
     1501 ttcttgcctt tgtgatgtac gcggccttgc cgtagttttc aacatatctg attccgtgat
     1561 aggactcatc gggctcggtg aaatcgggga acttgccgtt tgtccagtca aaattgccgc
     1621 tgtccacaat tactccgcct acctgaacgg cgtgaccgtc catgtattta gaggtggagt
     1681 gaatcacgat gtctgcgccg tactcaatgg gcttgcagag tatgggagtc gcgaaggtat
     1741 tgtctataat aaggggtatg ccgtgctttt tggcgaccct tgagaatttt tcaaaatcaa
     1801 aaacggtcag agcgggattt gcaattgtct cgccgaaaat cagctttgtg ttatctttaa
     1861 ttgcggcgtc tatttccgcc tcgtcggcgt cgcagtcaac aaaaacgcag tcaatgccgt
     1921 acttcttcaa ggtaacggca aaaagattga atgtgccgcc gtaaatctga gaggtgctta
     1981 caatgctgtc gcccgctttg cacaggttga gaacggcgat tagcgtcgca gcctgtcccg
     2041 aggatgtgca catggcggcc acgccgccct ccaaagcggc aattttattc tccaccgcca
     2101 taacggtggg gttcgcgaat ctcgagtaaa tcagcgcctt ggtaggctcg tcaaaaacgc
     2161 tgcccacctc tgttgtggag tcaaaaacat aggtcgtgct ttgaactatc ggaacattct
     2221 taggctcggt gttcttaggc tcatatcccg aatgaatgca ttttgtcaaa tccctcatat
     2281 taagacctcc ctaatttgac tatataatac agcaataatt ttacaatttc aatctttatc
     2341 gtaataccaa ggtgagattt ttgtcttttg agtggacaat aggtgctcct tcatatataa
     2401 ttgttctgta aattttaaca attatcagtt tggagggata ctatggataa taagcctgta
     2461 atcacaatca agccttacga actaaaatac agacgagatg tgcagcagct ctgtctcgct
     2521 aacgccaatc accttgataa gccgtttgag gacagaagat acatcctaat aatgtactgc
     2581 aactactaca tagagcagga gccggaaaac tgctttgttg ccgttaatga agaggacaag
     2641 gctataggct atattatctg cagcgagagc tacaaaaagt acagggagcg tttcaacagg
     2701 tactacatac cccaggcaag gtctttggga ttcagctact atgtaaaggc aaagctggat
     2761 atgctctctc acgctatgtt cgctaaaaaa tatcccgccc atcttcatat agatattgac
     2821 gacaattatc aaaggctcgg aatcggcaca aggcttatga atcagcttgt ggaccacctt
     2881 aaagcaaagg gcgtaaagcc gggtcttatg ctggttgtcg gcgcgaacaa cgaaaagggc
     2941 agaaacttct ataagaaata cggctacaaa gagctcgccg tcacaaagat gggcgtagcc
     3001 atgggaattg agctgtaata tagacaaaac aaaattcgga ggttactaat ggatatttca
     3061 tcagcagtgt tgttcgtggt tcttgccgcg gtttccatgt cttacgcatg gggaatgagg
     3121 ggtactatca tcggcggaga aaagggagct atgctgcccg gtgcggtaat gggcatgctg
     3181 attgcgcttt tctcctcatc agaggttctt aaagataatt tctttatgct ttcggcggtg
     3241 ggagccatgt caatgtatct gggaggctgc atgacatacg gcgaaaccct tggactttca
     3301 atgaactcca atcctcccga aaacttcaaa aagagcatca tcgcccttgt tgtaaaaggc
     3361 tttatctggt tctccctgtt cggagccttt gtgggcatcg cccttaactc cttcgccggt
     3421 cttttatata cggttaagga ctttatcctt atattcgcgc ttatccccgt atgcgctctt
     3481 gcgggatatt tccttctcaa caaacccctt gaccccaaga acggcaagtt cccgtttatc
     3541 tatttctcaa aaacaaggca ggagagctgg ggcggacttc tcggcatact ccttgtgctt
     3601 gtggctgtcg ccgcaaagaa taaagacagc tttaccttaa acctggttct cggcgccggt
     3661 ctctccggcg gcatcggctg ggctatcgcc cagaccctgc aggctgccgc aagactgcct
     3721 ttaataaagg gcaagagact ctttgaaaag atgaacagga agaaaacgat agacgcttgg
     3781 aagctgatgg aatattccct cggcgccatc ggcggcatcg gaattgccct gacgcttatc
     3841 gcccagcatg acaagttcag cgccatagcc gccgcaatag acagcagggg agctataaat
     3901 ctcccccttg agaagtacag cgccgttttc acagccgttt ggctcgttct tatagcgctt
     3961 gaccagcttc agttcatcat taagccccgc ttttccctgt cggagcttaa aaatcagaaa
     4021 attgtaaagg ctaccttatc cgaaagcgag tacgaaaagt gccttaaaga tgctgagaga
     4081 aaggaaagca gcccccactt taaacgctat gccttccttg tggaaaaagg cgagtttgcc
     4141 ctctactcac ttattccgat gttctttgtg ctttgcggtt cgtcgcttgt cggcaggctt
     4201 acctccttct tcatcctcta ccttgttgtc gttcaggagg tcgtgttcgg caggttccgc
     4261 ctcttaaagc acagaaggct cattaacacc tgccttatcc tcggcgccgt gctgatactg
     4321 ctttacgagc tggcgctgga caccaccttc agcatgatag caacgatgat aatgtacact
     4381 attttctatg acatcctcgg cattttgcat gttgtgccag ttgagaagga tgaaaaggta
     4441 aagcttggac ctttcaccat gctgaattac tgcgaccttt cggttttcgg atatatgagc
     4501 gtctccgggg tcgtcagcgt gcttcttctg gcgcttgcca tctatttcta aacattaaaa
     4561 agaaaaatcg gaacaatata gttttaagat gcgaaacaca ctcaggcaaa taaaaatcgg
     4621 aaatactgaa ataaacggag cagccgccct cgcgccgatg gcgggctgct ctgatttacc
     4681 cttcagagag ctgtgccgct ctttcggagc ttcatacacc gttaccgaaa tggtcagcgc
     4741 aaagggcttt gttatgggcg acagaaagtc tgaggagctc atgcggctcg gggaaaacga
     4801 acaccccgcg gcgattcaga ttttcggcag tgagcctttg tatatggcta aggctgcaga
     4861 gaaggctctt aagttttccc ccgacataat cgacataaac atgggc
//
LOCUS       DAONVQ010000132         4890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_12510_length_4890_cov_4.487901, whole genome shotgun sequence.
ACCESSION   DAONVQ010000132 DAONVQ010000000
VERSION     DAONVQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12510_length_4890_cov_4.487901"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="PLD98_07875"
     CDS             complement(<1..1219)
                     /locus_tag="PLD98_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HQA06197.1"
                     /translation="MADKTAIIDGIEVKIDNEPNLLELIRKAKIDLPTFCYHSEISIA
                     GACRMCMVEVEGMGIVPSCSTRVYDGMKVKTNTKQLRDMRKIIVELMLASHDQSCTTC
                     PKSGTCKLQRIAKQMGIVKVRFKQRTEFPEPDLSSPAIYRDSAKCVLCGDCVRVCKEI
                     QSVGVLDFANRGADAKVTPYFNKGIGDVECVNCGQCIKVCPVGALIPKYQTNEVWNVL
                     LDPEKYVVVQIAPAVRVAIGEYFGFKPGELTTGRMVSALRRLGFDKVYDTSFAADFTV
                     IEEGREFLERFKKGERLPQFTSCCPAWVKYAEQYYPDYLNNLSTCKSPQQMFGSLCKE
                     LLSKDMNIPRENIVVVSVMPCTAKKFEAQRPEFSVDGNPDVDFVLTTQELSVMIKERG
                     IKFAELENEEFDMP"
     gene            complement(1230..3119)
                     /locus_tag="PLD98_07880"
     CDS             complement(1230..3119)
                     /locus_tag="PLD98_07880"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQA06198.1"
                     /translation="MSDAKVLEHIKAAFDECMKNYKARIVVCGGTGCVANGAVVLYER
                     FKKVLKDKGLSFTVSLEREKADYLLSESGCQGFCQMSPLVTVYPAGVLYVRVKPEDVE
                     EIIDKTLVNNEIVERLLYHTPDTNTPCKTTQEIPFYATQMRKVLDMCGRIDAQDLDEY
                     IANGGYEMARKAILEMTDKEICDEILASGIRGRGGAGFPTGRKWDLTRVQKSDVKYVI
                     CNGDEGDPGAFMDSCVMEGTPHAVLEGLMIAAKATGATHGYVYVRTEYPLAVKRLRKA
                     VEDARKAGILGENIFGSGMNFDIEIKEGAGAFVCGEETALIASIEGKRGMPSPKPPFP
                     AEKGLFGKPTAINNVETLASIPMILRMGAKNYAAIGTEGSKGTKTFSLTGHVVNTGLI
                     EIPFGTTLREIVGKIGGGVVNDDGTVCGTDYKAVQIGGPSGGCLTQEHIDLPIEYESL
                     KEVGAMVGSGGLVVMNNQTCIVQIARFFMQFTQNESCGKCVVCREGTKQMLLLLDDIL
                     NGDATMETLDLLEELAKTVQTGSLCALGKSAPNPVLSTLRYFRDEYIAHIRDKRCPAG
                     KCEAFKQYSIDAAKCKKCGLCARKCPVDAITGSKTEPYTIDVEKCIKCGSCIDACKFD
                     AVLVM"
     gene            complement(3112..3615)
                     /gene="nuoE"
                     /locus_tag="PLD98_07885"
     CDS             complement(3112..3615)
                     /gene="nuoE"
                     /locus_tag="PLD98_07885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HQA06199.1"
                     /translation="MEDIITAKFDKVCEILDRHERNPHHLIAILQELQAEYTYLPEEI
                     MTYVSTALGIPPSSVYGVATFYSHFTLEPKGKYIIRICDGTACHVKKSEDILKALQKH
                     LGLGKNKKTTSDGLFTVETVACLGACSLAPAVVINGEVHGLLTPDSTIKLIEDIKKQE
                     QEGVENV"
     gene            complement(3660..4319)
                     /locus_tag="PLD98_07890"
     CDS             complement(3660..4319)
                     /locus_tag="PLD98_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HQA06200.1"
                     /translation="MSEKSTPNQVSANTLRRLPYYLSYLKSLKNTDVAYISAPVIASE
                     LDLNEVQVRKDIAAISSVAGKPKKGFEIDVLIRDIQAYIGCEDNNCGILVGVGHLGRA
                     LLAYKGFEAYGLKIIAAFDSDPSVVGTEIAGKPVYHIASLREFCQSTDAAIGIITVPA
                     EAAQSTCDLLVKSNIKAIWNFAPVMLNVPEGIIVQNENIASSLTIINKKLAEIAGSKQ
                     P"
     gene            complement(4571..>4890)
                     /locus_tag="PLD98_07895"
     CDS             complement(4571..>4890)
                     /locus_tag="PLD98_07895"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HQA06201.1"
                     /translation="CVMPSRNARHGHLFTWDGIININNAKYELDDSPIDPECQCPACR
                     TASRGYIRHLMKAGEMLGMRLAVIHNLYFYNNLMEKIRNALDNDTFEDFYFSNKDRLV
                     KRI"
BASE COUNT         1101 a         1190 c         1217 g         1382 t
ORIGIN      
        1 agggcatatc aaactcttca ttttcaagct ctgcgaactt aatgcctctc tccttaatca
       61 tgacggagag ctcctgagtg gtgagaacaa agtcaacatc ggggttgccg tcaacagaga
      121 actcggggcg ctgtgcctcg aacttcttgg ctgtgcaggg cattacggat actacgacaa
      181 tattctctct ggggatgttc atatccttgg agaggagctc cttgcaaagg gagccgaaca
      241 tctgctgggg tgacttgcag gttgagaggt tattgaggta gtcgggataa tactgctctg
      301 cgtactttac ccatgcgggg cagcaggatg tgaactgggg cagtctttcg cccttcttga
      361 atctctcaag gaactctctg ccctcttcga taactgtaaa gtctgccgcg aaggaggtat
      421 cgtaaacctt gtcaaagccg agtcttctga gggccgaaac catgcggcct gtggtgagct
      481 cgccgggctt aaagccgaaa tactcaccga ttgcgactct gacagcgggt gctatctgaa
      541 cgacaacata cttttcgggg tcaaggagaa cattccaaac ctcgttggtc tgatatttgg
      601 ggataagtgc gccaacgggg caaaccttga tgcactggcc gcagtttacg cactcaacat
      661 cgccgatgcc cttgttgaag taaggtgtaa ccttagcgtc cgcgcctctg ttagcgaaat
      721 caagaacgcc aacggactgg atttccttac atactcttac gcagtcgccg cagagaacgc
      781 acttagcgga atctctgtag attgcgggtg agctgaggtc cggctcgggg aactcggtgc
      841 gctgcttaaa tcttaccttt acaatgccca tctgctttgc gattctctgg agtttgcagg
      901 taccgctctt ggggcatgtg gtgcagctct ggtcatggct tgcgagcata agctcgacaa
      961 ttatttttct catgtctctg agctgctttg tgtttgtctt gaccttcatt ccgtcataga
     1021 ctcttgtgga gcaggaggga acgatgccca tgccttcaac ctcaaccatg cacatacggc
     1081 aggcgcctgc tatgctgatt tcggagtgat agcagaatgt gggcaggtca atcttagctt
     1141 ttctaatgag ctcaagcagg ttgggctcgt tatctatctt aacttctatg ccgtcaataa
     1201 ttgctgtttt atctgccatt ggtttcacct cacattacta atacagcatc aaacttgcaa
     1261 gcgtctatgc agcttccgca tttaatgcac ttttcaacat caattgtgta gggctctgtc
     1321 tttgaaccgg taattgcgtc aacggggcat tttcttgcgc acagaccgca cttcttgcac
     1381 tttgccgcat cgatgctgta ctgcttaaac gcttcgcact tgcctgccgg gcagcgctta
     1441 tccctgatgt gcgcgatata ttcatctctg aagtatctga gagttgagag aaccgggttg
     1501 ggagcgctct tgccgagggc gcagagcgaa cctgtctgaa cggtctttgc gagctcctcg
     1561 agaaggtcga gggtttccat tgttgcgtcg ccgttgagaa tgtcgtcaag gagaaggagc
     1621 atctgctttg ttccctcacg gcatacaacg cacttgccgc aggactcgtt ctgtgtgaac
     1681 tgcatgaaga accttgcgat ctggactatg caggtctggt tgttcataac gacaagaccg
     1741 ccggagccga ccattgcgcc aacctctttg aggctttcgt actcgatggg caggtcgata
     1801 tgttcctgag taaggcatcc gcctgagggg ccgccgatct gaacagcctt gtagtcggtg
     1861 ccgcaaacgg ttccgtcgtc gtttacaacg ccgccgccga ttttgccgac gatttctctt
     1921 aatgtggtgc cgaagggaat ctcaataaga cctgtgttaa cgacatgacc tgtaagcgag
     1981 aaggtctttg tgccctttga gccttcggtg ccgatggctg cgtagttctt tgcgcccatt
     2041 cttaaaatca tcgggataga ggcaagggtc tcaacattgt tgatggcagt gggcttgccg
     2101 aaaagaccct tttctgccgg gaagggcggc ttcgggctgg gcatgcctct cttgccttcg
     2161 attgaagcga tgagggctgt ttcctcgccg catacgaatg cgcctgcgcc ttccttaatc
     2221 tctatatcaa agttcatgcc ggagccgaaa atattttcgc caagaatacc ggcttttctt
     2281 gcgtcctcaa ctgctttcct gagtctctta acagcgagag gatactctgt acggacatat
     2341 acatagccgt gggttgcgcc ggttgccttt gcggcgatca taagaccctc aagaaccgca
     2401 tggggtgtac cctccataac gcagctgtcc atgaatgcgc cggggtcgcc ttcgtcgccg
     2461 ttgcagataa catattttac atcgctcttc tgcactctgg ttaaatccca tttgcgtcct
     2521 gtggggaaac ctgcgccgcc gcggcctctg atgccggaag caaggatttc gtcgcagatt
     2581 tccttatcgg tcatctcaag gattgccttg cgtgccatct cataaccgcc gttggctata
     2641 tactcgtcaa ggtcctgagc gtcgattctg ccgcacatat ccagaacttt tctcatctgg
     2701 gttgcgtaga aggggatttc ctgcgtggtc ttgcacggag tatttgtatc gggggtatga
     2761 taaaggagtc tttcgactat ctcgttgttt acaagagttt tgtcaatgat ttcttcaaca
     2821 tcctcaggct ttactcttac ataaagaacg cctgcgggat atacggttac cagcgggctc
     2881 atctggcaga agccctgaca tccgctttcg gaaagcaggt agtcagcctt ctctctctcc
     2941 agagaaacgg tgaatgacag acccttatct ttaagcacct tcttaaagcg ctcataaagg
     3001 acaacggcgc cgttggcaac gcagccggtt ccgccgcata ccactatgcg ggccttataa
     3061 tttttcatgc attcatcgaa cgcggctttt atatgttcaa ggaccttagc atcagacatt
     3121 ttctacacct tcctgttcct gcttcttaat atcttcgatg agctttattg ttgagtcggg
     3181 cgtcagcaag ccgtgaacct caccatttat aacaactgcg ggagcaaggc tgcatgcgcc
     3241 gaggcatgcg actgtctcaa cagtaaacag gccgtcggaa gttgtttttt tgttcttgcc
     3301 gaggccgagg tgcttctgca gagcctttaa tatatcctcg gacttcttaa catggcatgc
     3361 tgtgccgtcg cagatgcgga tgatatattt gcccttgggc tccagtgtga agtgggaata
     3421 gaatgtggca acaccgtata ccgatgaagg aggaataccc agagctgtgg agacataagt
     3481 cataatctcc tcaggcagat aggtatattc cgcctgcagt tcctgaagta tggcgattaa
     3541 atgatgcgga tttctctcat gtctgtcaag aatctcacaa accttatcga acttcgctgt
     3601 gataatatct tccatcataa gactcctttt ttccaaattg attatatact ttcggagcat
     3661 taaggctgtt tggagcctgc aatctccgca agtttcttat taattattgt aagggacgag
     3721 gctatgttct cattctgaac gattatgccc tcggggacat tgagcataac gggggcaaag
     3781 ttccagattg cctttatgtt cgactttaca agcaagtcgc aggttgactg ggctgcctcc
     3841 gcgggaacgg ttatgatgcc gattgcggcg tcggtgcttt ggcagaattc ccggagggaa
     3901 gcgatgtgat acacgggctt tcccgcgatt tccgttccga ctaccgaggg gtccgaatcg
     3961 aacgcggcga tgattttgag tccgtatgcc tcaaagcctt tgtaggcaag aagcgctctg
     4021 cccagatgac ctacgcccac gagtatgccg cagttattgt cctcgcagcc tatgtaggct
     4081 tgaatgtctc ttatcagaac atcgatttca aagccttttt tgggcttgcc cgctacggag
     4141 ctgattgccg cgatgtcctt tctgacctgc acctcgttaa ggtccagctc gctcgctatg
     4201 acaggggcgg agatataagc aacatcggtg tttttgaggc tcttaaggta gctcagatag
     4261 tatggcaaac gccttaaagt gtttgccgag acctgattcg gagtactctt ttcgctcatg
     4321 cccggccctc catttccctg acttttttct tcggttttcg atataattat agcatatttt
     4381 cgtgaatgtt aaaataatat cagtaaaatt atatcgataa aacctaaatt attttaaatg
     4441 cataagtaca aaaagtcaat agaaatgaaa atattattgt ttttgggcaa aaaaatgagc
     4501 caaatccata aaattcagac ttggcttcta tatattttag acactttttc aggggtcaat
     4561 aaacgggata ttatatcctt tttacgagtc tgtctttatt ggaaaaatag aaatcttcaa
     4621 atgtatcgtt atcaagggcg tttctgattt tttccatcag attgttgtag aaataaaggt
     4681 tgtggataac cgcaaggcgc atgcccagca tctcccccgc tttcattagg tgcctgatat
     4741 atcctcttga tgcagtgcgg caggcggggc attggcattc ggggtcaatg gggctgtcgt
     4801 caagctcgta ctttgcgttg ttgatattga tgatgccgtc ccatgtaaac agatgaccgt
     4861 gccttgcgtt tctgctcggc attacgcagt
//
LOCUS       DAONVQ010000133         4597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_13468_length_4597_cov_4.058124, whole genome shotgun sequence.
ACCESSION   DAONVQ010000133 DAONVQ010000000
VERSION     DAONVQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13468_length_4597_cov_4.058124"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLD98_07900"
     CDS             <1..357
                     /locus_tag="PLD98_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HQA06202.1"
                     /translation="AALCGDNLHIYSGNDDQILAIMAHGGKGVISVLANVVPKAAHDI
                     AAAYLEGDVERSRKLQFEYLDLCNDLFIDVNPIPVKEALNMMGFKVGPCRRPLAPLSK
                     ANREKLAATLKKHNLI"
     gene            438..1196
                     /gene="dapB"
                     /locus_tag="PLD98_07905"
     CDS             438..1196
                     /gene="dapB"
                     /locus_tag="PLD98_07905"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HQA06203.1"
                     /translation="MLRIILRGCSGKMGRVVTEVASTQEDLRIVAGVDVIDNASMPYP
                     IFTSFSEVPVEAEVVVDFSNPSNLDDFLNFLTKKKMPAVIASTGFSDEQLERIAEAAT
                     ETPIFMASNMSIGINLLAELAKTAAKVLGGSFDIEIVEAHHNQKLDAPSGTAKTLAKA
                     VEAGLDYEPIYTYDRHDRRAKRDKHEIGMHAIRGGTIVGEHEIIFAGNDEVIKLSHSA
                     LSKNIFAEGALNAARYIVKKQPGLYSMHDLVSES"
     gene            1211..1684
                     /locus_tag="PLD98_07910"
     CDS             1211..1684
                     /locus_tag="PLD98_07910"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQA06204.1"
                     /translation="MENNTSLELLEDVTLITLQDCPADAAKIAYLFKIIADEGIDVDM
                     ISQIATPKRTFELSFTVSGDDFGKILDIATKLRKEEPELKLNVSNGNSKITVSAEWMK
                     NCPGIAAKVLDIVSKASADIMMITTSETEISILVPQADGDNTLAALEKEFVECSA"
     gene            complement(1744..2835)
                     /locus_tag="PLD98_07915"
     CDS             complement(1744..2835)
                     /locus_tag="PLD98_07915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQA06205.1"
                     /translation="MITISLAMIVKNEENTLGNCLESVKDIVDEIVIVDTGSTDKTKE
                     VASKFTDKIFDFEWIDDFSAARNFACSKATKEYVMWLDADDVILPEDKRKFLELKQTL
                     SPDIDMVFMKYNVGFDENGNVTFSYYRERLARRDKGFIWMEPVHEYLAFTGKSINVDI
                     AVTHTKKHRGFSDRNLRIYEKQLKEKGELSPRGTYYYARELKDNGRYEEAADMFLKFL
                     DSNKGWVEDNINACLERSKCLLLLGRWEESFHALTRSFLYDLPRAEIVCQIGYYFKFH
                     RAYHQAIYWFETALRLQKPKDSWGFFYDDYWGYIPALELVVCYDAIGDTLRANHYNEI
                     AASFKPDSQAVEHNRQYFKAKLSGTEPNQ"
     gene            3006..3866
                     /locus_tag="PLD98_07920"
     CDS             3006..3866
                     /locus_tag="PLD98_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQA06206.1"
                     /translation="MKLLILTKKHLLVAIAVCIALCLAVIGVSKGLSVAVSAPKREIP
                     IYSVATDEKKVALTFNAAWSDSDIDEIISVLKKFKVKATFFIVGEFADKYPEAVRKLS
                     NAGHEIANHSDKHANMTKMKYEDIKADIAASNEKLKKLTGKTPDLFRAPYGDYNNDVV
                     KAAKSLSMFAIQWDVDSLDYKDLSAEDIKKRVVPKVQNGSIVLFHTDTLSTAKALPSI
                     IKELQEKGYELVTVGELIYKENYSIDHTGKQSKVTAPTTAPPTTAEQTTAVPTTAAET
                     KATEPTTKKK"
     gene            complement(3902..>4597)
                     /locus_tag="PLD98_07925"
     CDS             complement(3902..>4597)
                     /locus_tag="PLD98_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HQA06207.1"
                     /translation="SPGMHISLGRFRSEEEEKDNQGDRNFALQAVERGFAALIIEQRN
                     FGECSGPENFRRGGCHIPAMQAILLGRTTIGERALDVSRAIDIAADMPFADTDRIGIM
                     GNSGGGTATYYASCTEDRIKALMPSCAFCTVEDSIFSISHCQCNFMPGMLKYFEMADL
                     AVLLAPRPLVVVYGKKDNIFPEHGVLKAYEVAKEIYKKFGAEDKIRLVPGDEGHRFYE
                     YPSWDVFREVTGW"
BASE COUNT         1233 a         1100 c         1107 g         1157 t
ORIGIN      
        1 gcagcccttt gcggcgacaa tctgcacatt tactcgggca atgacgacca gatactcgcc
       61 atcatggctc acggcggcaa gggcgtaatt tcggttctcg caaatgtggt gccgaaagca
      121 gctcacgata ttgcggccgc ttatcttgag ggcgatgtgg agcgttcaag gaaactgcag
      181 ttcgaatatc ttgacctttg caacgatttg ttcatcgatg taaacccgat tcccgtaaaa
      241 gaagccctta acatgatggg cttcaaggtc ggtccgtgca gaaggccctt ggctcccctg
      301 tcaaaggcta acagagaaaa actggccgca acgcttaaaa agcacaatct tatttagtaa
      361 aaagcctaaa cgggtgaccg tttaggctaa aatatagatt ctgctttttg gaaattgagt
      421 ataaaggatg attcaggttg cttaggatta tattaagagg ctgttccgga aaaatgggca
      481 gggttgtgac agaggtcgcg tccacacagg aggatttgag aattgtcgcc ggcgttgatg
      541 ttatagacaa cgcctcaatg ccctacccaa tattcacgag cttctctgag gttcccgttg
      601 aagccgaagt tgtcgtggat ttctccaatc cctcaaacct tgatgatttt ttgaacttcc
      661 ttacaaaaaa gaaaatgcct gcggttatag cgtccacggg cttttccgac gagcagcttg
      721 agaggattgc cgaggcggca acggaaactc ccatattcat ggcttcaaat atgtctatcg
      781 gaataaacct cctcgctgag cttgcaaaaa ccgcggccaa ggtgctgggc ggctcctttg
      841 atattgaaat tgttgaggct catcacaatc aaaagctgga cgctcccagc ggaaccgcaa
      901 agaccctcgc caaagcggtt gaagcgggtc ttgattacga gcccatatac acatacgacc
      961 gtcatgacag acgggcaaag cgcgacaagc atgaaatagg catgcatgcc ataagaggcg
     1021 gcacaattgt cggagaacat gagattatct ttgcgggaaa cgacgaggta ataaaactct
     1081 cccacagcgc actgtcaaag aacatcttcg cggagggcgc gctgaacgcc gcaagataca
     1141 tagtaaagaa acagccgggg ctgtattcga tgcatgattt agtttcagaa tcctaataag
     1201 gagggtcaca atggaaaaca acacttcgct tgagttactt gaagatgtaa ctctgataac
     1261 cctgcaggat tgccctgccg atgcggctaa aattgcttat cttttcaaga ttattgccga
     1321 tgaaggcatt gatgtggata tgatttctca gatagccacc cccaaaagga cctttgagct
     1381 ttcctttacc gtcagcggcg atgacttcgg caagattctg gacattgcca caaagctgag
     1441 aaaggaagag cctgaactta aactcaatgt aagcaacggc aattcaaaaa tcaccgtttc
     1501 ggcagaatgg atgaagaact gccccggaat cgcagcaaag gtactggata tagtatcgaa
     1561 agcctctgcg gatattatga tgattaccac atccgaaact gagatttcga ttcttgtgcc
     1621 ccaggcagac ggcgacaata ccttagccgc gcttgagaaa gaatttgtgg aatgctccgc
     1681 ttagtataat ccaaaatcaa gcaccccgag aataactcgg ggtgtttttg cattttaggc
     1741 ttattattga tttggctccg tgccgctgag ctttgctttg aaatactgcc tgttgtgctc
     1801 cactgcctga gaatccggct taaagctggc ggcaatctcg ttgtaatgat tcgcgcgcag
     1861 tgtatcgcct atggcgtcgt agcataccac aagctcaagg gcgggaatat agccccagta
     1921 gtcatcgtag aaaaatcccc agctgtcctt cggcttttgc agcctgagtg cggtttcaaa
     1981 ccagtaaatc gcctgatggt atgcccgatg aaacttgaag taatagccta tctgacatac
     2041 gatttccgcc ctcggaaggt catagagaaa actccttgtg agggcatgga aagactcctc
     2101 ccatctgccc aaaagcagaa ggcattttga cctttcaagg caggcgttga tattgtcctc
     2161 cacccagccc ttgttggagt caaggaattt aaggaacata tcggcggctt cttcatagcg
     2221 gccgttgtcc ttgagttccc gcgcgtagta gtatgttccg cgggggctga gttctccttt
     2281 ttctttaagc tgcttttcgt agattctaag gttcctgtcg ctaaagcccc tgtgcttttt
     2341 tgtgtgggta acggctatat ccacattgat tgacttgccc gtaaaggcaa gatattcgtg
     2401 aacgggctcc atccatataa agcccttgtc acgccttgcg agcctttctc tgtaatagga
     2461 aaaggtcaca ttgccgtttt cgtcaaagcc cacattgtac ttcataaaaa ccatatctat
     2521 gtcgggggaa agggtttgtt tcagctccaa gaatttgcgc ttatcctcgg gcagaatcac
     2581 atcatcggcg tcaagccaca taacatattc ctttgtggcc ttggagcagg caaaattcct
     2641 cgcggcggaa aagtcgtcta tccactcaaa atcgaatatc ttgtcggtaa atttgcttgc
     2701 cacctctttt gttttgtccg tagaacccgt atccactata actatttcat ccacaatgtc
     2761 ctttacgctt tcaaggcagt tgccgagggt gttctcctca tttttaacaa tcattgcaag
     2821 acttattgta atcacctgaa aaccctcttt ccgctaaagt cagattccct ttcagtctat
     2881 gcggggaaaa gtcgtaccgt gacgcaaagc caatctaaag gacaaaacag gtatgtacgg
     2941 ggaagtttgg acatatatat tgatggaagg gcaagccatt aaattttgaa ttcggggtga
     3001 atagattgaa gctgctgata cttacaaaaa aacatctgct tgttgcaatt gcggtttgca
     3061 ttgctttatg ccttgccgta attggagttt caaagggtct gtcggtcgcc gtatcggctc
     3121 cgaaacgaga gattcccatt tacagcgtgg caacggatga gaaaaaggtc gcccttacct
     3181 ttaacgccgc gtggagcgac agcgacattg acgagattat ttcggtactt aaaaagttta
     3241 aggttaaggc aacattcttc atcgtgggag aattcgcgga caaatatccc gaagctgtta
     3301 ggaagctgag caacgcaggt catgaaatcg caaaccactc tgacaaacat gcgaacatga
     3361 ccaaaatgaa atatgaggac ataaaggcgg atattgccgc gtccaacgaa aaattaaaga
     3421 agctgacggg caaaactcct gatttgttca gggcgcctta cggcgactac aataatgatg
     3481 ttgtaaaggc cgcaaagtcg ctctcaatgt ttgcgattca gtgggatgtc gacagccttg
     3541 actacaagga tttatctgct gaggacataa agaaaagggt tgtgcccaag gttcaaaacg
     3601 gctccatagt tttattccac accgacaccc tttcaaccgc aaaggcgctg ccgtcaatca
     3661 ttaaggagct gcaagaaaag ggctatgagc ttgtaaccgt tggcgagctg atttacaagg
     3721 agaattacag cattgaccat acaggcaagc agagcaaggt aacggcgccc acaaccgcgc
     3781 cgcctacaac ggcagagcaa acaacggcag tacccacaac cgcggccgaa acgaaagcca
     3841 cagagcctac tacaaagaag aaataaataa agccgccctt tagggcggct cttttatctt
     3901 tttaccagcc tgtgacttct ctgaaaacat cccatgaggg gtattcatag aacctgtgac
     3961 cttcgtcgcc ggggacaagc ctgattttgt cctccgcgcc gaatttctta tagatttctt
     4021 tggcaacctc gtatgccttc agaacgccgt gctcagggaa gatattgtcc tttttgccgt
     4081 agaccacgac aaggggacgg ggcgctagaa ggaccgcaag gtcagccatt tcaaagtatt
     4141 taagcatgcc gggcataaag ttgcactggc agtgggatat gctgaaaatc gagtcctcta
     4201 ctgtgcagaa ggcgcaggag ggcataagag ccttgattct gtcctctgtg caggaggcat
     4261 aataggttgc ggttccgccg ccgctgttgc ccataatgcc gattctgtct gtatcggcaa
     4321 agggcatgtc ggccgctatg tcgatggctc ttgagacatc gagagctctc tcccctattg
     4381 ttgttcttcc aagaagtatt gcctgcattg cggggatgtg gcagccgccc cttctaaaat
     4441 tttcgggacc tgaacactcg ccgaaattcc tctgttcgat aatcaaagcg gcaaagcctc
     4501 tttccaccgc ctggagggca aagtttctgt cgccctgatt atccttttcc tcctcctcgc
     4561 ttctgaaacg acccagggaa atgtgcattc ccgggga
//
LOCUS       DAONVQ010000134         4512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_13770_length_4512_cov_4.610725, whole genome shotgun sequence.
ACCESSION   DAONVQ010000134 DAONVQ010000000
VERSION     DAONVQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13770_length_4512_cov_4.610725"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1836
                     /locus_tag="PLD98_07930"
     CDS             253..1836
                     /locus_tag="PLD98_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA06208.1"
                     /translation="MSNEIMTEEFTEQQLNYVYENRRYVRRKEVVGYVLWDMAQSFNI
                     NSYSQRFVNTILQISFRYQQIISFVNGIWDIINDIFTAAIVDKTRTRWGKFKPYLVLL
                     AIPGTAGACLYWMLPLFFRGTGTEDFSKFIAYFILAIVREGIGTFVSIAQGGIISTIT
                     PHPVERTRILTLANYFSGMFGEKLPEQIMTILLDLISNNVIKPKKLTISELLLRLFVG
                     MGVFTSIVSGMTSLWFNTIVRERVAQSVKTPSIVQGIKSIINNKPVLLLTLSQILSSF
                     SIGGSRSDYYIDVLNFASMGYVTGFPGALLGPVPFLIVPWFRRKFSSRTLYLLSNYLG
                     SVLMVPIFIIGSIGGKKNGLYKNRIAMMIMMTIHETIIAVFYGLRSVIPTELYNEAMD
                     YCEWKNGYRTEAMTSVARGLASKLAGIFTNLITLQIKRWIKYDQTAYIRGAAQSDSTK
                     YWLFASYAILPLLTGGFGMIPIFFYDLSGHKREKMYAELLERREKISKTASQADKETM
                     EKIAQEQLKVAEINKNKKL"
     gene            complement(1911..2474)
                     /locus_tag="PLD98_07935"
     CDS             complement(1911..2474)
                     /locus_tag="PLD98_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQA06209.1"
                     /translation="MILLKLFWSFFQIGLFSIGGGMAAIPLIRNQVVSINGWLTLTEF
                     TDLVTIAEMTPGPIGINSATFVGIRIAGLPGAIAATAGCVLPSCIIAAILAWVYYRFK
                     NLAVAQGVLDALRPAIVALIASAGLTILILAIFGEGGFKADIKEINFVSVLIFVSSLF
                     VLRKWKVNPVFVMLGAGIIGGAIYMFC"
     gene            complement(2471..3055)
                     /locus_tag="PLD98_07940"
     CDS             complement(2471..3055)
                     /locus_tag="PLD98_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQA06210.1"
                     /translation="MKDKKKKVYLKLFTSAFYLSAFTFGGGYVIIPLMKRKFADELKW
                     IEEKEMLNLTAIAQSSPGPVAVNAAMLLGYRVAGAPGALVTVLGTILPPFITLSVVSL
                     FYAAFRDNPIVNAVLKGTQAGVAAVIADVSLSLGKNVVKERDILSVIIMAAVFVLIFF
                     LKVNVMLIILACAIIGAARILFKMKKEAKGGDEQ"
     gene            complement(3169..3963)
                     /locus_tag="PLD98_07945"
     CDS             complement(3169..3963)
                     /locus_tag="PLD98_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HQA06211.1"
                     /translation="MEKIIDIQHLSKTFGNNVVLKDIDFSVNKGEVVCIIGSSGSGKS
                     TLLRCINLLEKPSGGKIIYKSENILDDKHDVREYRTKLGMVFQQFNLFNNHDVLGNCT
                     VGQINVLKRSKEEAKKIAMKYLQVVGMDQYINAKPRQLSGGQKQRVAIARALCMEPEV
                     MLFDEPTSALDPEMVGEVLKVMRDLAESGLTMLIVTHEMGFARDVADRVVFMDKGVIA
                     EEGPPEQIFNNPKQERTREFLSRTLQQNPAYIPSDSSEAQKIVKAI"
     gene            complement(3981..>4512)
                     /locus_tag="PLD98_07950"
     CDS             complement(3981..>4512)
                     /locus_tag="PLD98_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HQA06212.1"
                     /translation="MVIYYGSKQALDIDMTPLFAGIFIVSINTGAYMAEIVRGGIISI
                     DRGQFEASYAIGMSHLQTMTKVVLPQVIRNILPATGNEFVINIKDTSVLNVISVTELF
                     FQTKSITGNNFKYFETFFITCVIYFVLTYSVTRILRFIEKKLDGPENYTLLDDELEEE
                     KPKPAVLQNQNIIGKH"
BASE COUNT         1220 a         1074 c          990 g         1228 t
ORIGIN      
        1 cactgattag catttgttat tctgcggtat atatctcaaa aaggcaccct gcgtttttgc
       61 agagtgcttt tttattcttg ctattcttgc tttaaaagca gtgattgact tttattataa
      121 ttttttggta ttcttaatat agtttaatat gcaactttaa caaaaaatgg gattcggcga
      181 catcgctttg ccgaatattg ttctttctgc gcaagacatt ttttattcta aggcggaaaa
      241 gggcgtgatg tattgtcaaa cgaaataatg accgaggaat ttaccgagca gcagcttaat
      301 tatgtctacg aaaaccgacg ctatgtaagg agaaaagagg ttgtgggcta tgtgctgtgg
      361 gatatggcac agagctttaa cataaactcc tattcccagc gctttgtaaa taccatactt
      421 cagataagct tcagatacca gcagataatc tcctttgtca acggcatttg ggatattata
      481 aacgacattt ttaccgccgc aatcgtagat aagacccgca cccgctgggg caaattcaag
      541 ccgtatcttg tactccttgc catccccggc accgcgggtg cctgccttta ttggatgctc
      601 ccgctgttct tcagaggcac gggcacagag gattttagca aattcatagc ctatttcatt
      661 cttgccattg tgcgagaagg aatcggcacc tttgtcagca tcgctcaggg cggaattatc
      721 tcaaccataa ccccgcatcc cgttgagcga acaagaattc taacccttgc gaactatttc
      781 tcgggaatgt tcggcgaaaa gctgcccgag cagataatga ccattctcct tgaccttata
      841 agcaacaatg taataaagcc aaagaaactg acaatcagcg agctgctttt aaggctcttt
      901 gtgggaatgg gcgtgttcac aagcattgtc agcggcatga cctctttgtg gtttaacacg
      961 atagtcaggg agagggttgc ccagtcggtc aaaaccccca gcattgttca gggaattaag
     1021 tccatcataa acaataagcc ggttctgctc ctgacgcttt cgcagatttt aagctccttc
     1081 tcaatcggcg gcagccgctc agactattac atagatgttc tgaacttcgc gtccatgggc
     1141 tatgtaacgg gcttccccgg cgcgctctta ggccccgtgc ccttcctcat agtgccttgg
     1201 ttccgccgca aattctcatc ccgcaccctg taccttttga gcaattatct gggcagcgta
     1261 ttgatggttc ccatatttat catcggctca atcggcggca agaaaaacgg cctttacaaa
     1321 aacagaatag ccatgatgat tatgatgacg atacacgaaa caatcatagc cgttttctac
     1381 ggtctcaggt cggttattcc caccgagctt tacaatgagg caatggacta ctgcgaatgg
     1441 aaaaacggct acagaacgga ggcaatgacc tcggtcgcaa ggggacttgc aagcaagctg
     1501 gcgggcatat ttacaaatct tattaccctc cagattaaac gctggataaa atacgaccag
     1561 accgcttata tcaggggagc ggctcagagc gacagcacaa agtactggct cttcgcgtcc
     1621 tatgcgattt tgcccctgct tacgggagga ttcggcatga taccgatatt cttctacgac
     1681 ctgtcgggtc acaagaggga gaaaatgtac gcagagcttt tagagcgccg cgagaaaata
     1741 tctaaaaccg cctcccaggc agacaaggag acaatggaaa aaatcgcgca ggaacagctc
     1801 aaagtcgctg aaatcaacaa gaacaaaaag ctgtaaaaaa gttaagagcc cgttttcaga
     1861 aaaataaaag tcccgactaa aaggctgtcg ggacttttta ttttttaaat tcagcaaaac
     1921 atatatatcg cgccgccaat tatgcccgcg cccagcatta caaatacggg gtttaccttc
     1981 cactttctca aaacaaataa ggaagataca aaaatcaaaa cagaaacaaa gtttatttct
     2041 ttaatgtcag ctttaaagcc accctccccg aagatagcga gtataagaat agtaagacct
     2101 gcagaggcta tcaaggctac tattgcgggt cttaaagcat ccaaaactcc ctgtgcaacg
     2161 gcgagatttt taaatctgta atatacccag gcaaggatag ccgcgataat gcatgaagga
     2221 agaacacagc cggctgtcgc ggcaattgcc cccgggagcc ccgcaattct gattcccaca
     2281 aaggtagcgg aatttatgcc gatgggaccc ggtgtcattt ccgcaatggt tacaaggtcg
     2341 gtaaactctg taagcgtaag ccagccgttt atgcttacta cctgatttcg tataagcgga
     2401 attgccgcca tgcctccgcc tatgctgaaa agtccgattt gaaaaaagct ccaaaagagc
     2461 ttaagcagta tcattgttca tcgccgccct ttgcctcttt tttcatctta aagagaattc
     2521 ttgccgctcc gataattgca caggctaaaa ttatgagcat tacattgacc tttaggaaaa
     2581 atatcaaaac aaacacggct gccattatta ttacagaaag tatatccctt tcctttacga
     2641 catttttccc aaggcttaaa cttacatcgg cgataacagc cgcaacgcct gcctgcgttc
     2701 ccttcagcac ggcatttaca atggggttgt cacggaacgc ggcgtaaaaa agggagacaa
     2761 cagacaatgt aataaagggc ggcaaaatcg tacccaaaac ggttacaagg gcgccgggcg
     2821 cccccgcgac acgatagccc aacagcattg cggcattgac ggcaacagga cccggggaag
     2881 actgagcgat tgccgttaag ttcagcattt ccttttcttc aatccattta agctcatccg
     2941 caaactttct tttcatcagc ggaatgatta catacccgcc gccaaaggta aaggcactga
     3001 gataaaatgc tgaggtgaat agctttaaat ataccttctt ttttttatcc ttcaaatcat
     3061 cgctcccttt aaatgccttt ggtataagat tatataataa aaccgaaaaa gagcaaagga
     3121 tttaagcgcc aaaaaaacag gcccttttcg gagcctgttt ttttatgctt atattgcctt
     3181 tacaatcttt tgtgcttcag agctgtcgga ggggatgtaa gcggggtttt gctgaagtgt
     3241 cctgcttaaa aactcccttg tgcgctcctg cttggggttg ttgaatattt gctcgggcgg
     3301 gccttcctca gcgataacgc ccttgtccat aaataccaca cggtctgcga catccctcgc
     3361 aaagcccatc tcatgggtta ctattagcat ggtaagtccg gattccgcaa ggtctctcat
     3421 gactttaaga acctctccca ccatttccgg gtccagagcg cttgtgggct cgtcaaagag
     3481 cattacttca ggctccatgc aaagagccct ggctatcgcc acgcgctgct tttgtccgcc
     3541 ggagagctgt ctgggcttgg cgttgatgta ctggtccatg ccgacaacct gcaggtattt
     3601 catggcgatt ttctttgcct cttccttaga gcgctttaag acatttatct gtcccaccgt
     3661 gcagttgccc agaacatcat ggttgttaaa gaggttaaac tgctgaaaaa ccatgcccag
     3721 ctttgttctg tactcgcgca catcgtgctt gtcgtccagg atgttttcac tcttgtatat
     3781 tatctttccg ccgctgggct tttccagaag gtttatgcag cgaagaaggg ttgacttgcc
     3841 ggagccggag gagcctatta tgcagacgac ttctcccttg ttgaccgaaa agtcgatgtc
     3901 tttcaagacc acattattac cgaaggtctt gcttaagtgc tgaatatcaa taatcttttc
     3961 catcgttccc cctccttctt ttaatgtttc cctattatat tctgattttg caaaacagcg
     4021 ggtttgggtt tttcttcctc aagctcatcg tcaagcaggg tatagttttc ggggccgtcc
     4081 agtttctttt ctatgaaacg aagaattctt gtgaccgaat aggtcagaac aaaatatatt
     4141 acacaggtta tgaagaaggt ttcaaagtat ttaaagttgt tgcccgtaat tgattttgtc
     4201 tggaagaaca gctctgtgac ggagataaca ttcagaacgg aggtgtcctt aatgttgata
     4261 acgaattcgt tgccggttgc gggcagaatg tttcgaatta cctgcggcaa taccactttc
     4321 gtcattgttt gcaagtggct cattcctatg gcgtaggagg cctcaaactg tcctctgtct
     4381 attgaaatta tgccgccgcg gactatttcc gccatatacg cgcccgtgtt tattgagaca
     4441 atgaagatac ccgcaaacag gggagtcatg tcgatgtcaa gagcctgctt ggagccgtag
     4501 taaataacca tt
//
LOCUS       DAONVQ010000135         4483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_13875_length_4483_cov_4.213640, whole genome shotgun sequence.
ACCESSION   DAONVQ010000135 DAONVQ010000000
VERSION     DAONVQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13875_length_4483_cov_4.213640"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1702)
                     /locus_tag="PLD98_07955"
     CDS             complement(<1..1702)
                     /locus_tag="PLD98_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06213.1"
                     /translation="MSKRILKALSAIVVIALLFSSFRVNIASTKISTVAQESAAAGAE
                     SEKVAADVNAEAEANKEEKPETVVKTQSLPIQPKEGVKQYPIIVLPGISQTISYLVDE
                     NGEKILNADGQELKGGLLIIDTAGLVQKIFKTVFFPLVKSLISQKDAGLSQAIYELGL
                     DLFSTQASKPDGTTVNRFAIDEYNYPLSEFSEEDRDYFYRQIPMQDYAQAVGEEYIYV
                     FSYPLLGNPIENGKKLHEFIQMVKEQQNTDKVNIASVSMGGTVLASYLNYEEADYNDI
                     NCIINVVSLLSGTDIIADFLTRGYEGFNLEDQFFFSEYLPEVFKELTGSPALGYVANI
                     LVRLLPRKVLESMITAAADAAFGQLIVNSPQFWAMIPNDRYDALAEKYLLDPERVLLK
                     EKTDAFHEAQSNLSVNLLKARDEYGVKVGFIAGYNLTYLDGDYNFFGAVKSTFKNSSD
                     AIIDIDSATLGATFAPAGKQLPQDYIDSLTDKSFVSPDKNIDLSTALMPETTWVFKNQ
                     HHEVGRNITIIRLAVQFVIGNLDNVYSDPVNYPQFNIGRYSKAITNSLLPMAYKVLAE
                     P"
     gene            complement(1824..2417)
                     /locus_tag="PLD98_07960"
     CDS             complement(1824..2417)
                     /locus_tag="PLD98_07960"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HQA06214.1"
                     /translation="MSAIFDMINMALYTCLIRNVIFTGGFGATESVRMAAKPKYLFHT
                     SGFIVYFSVLVALICRTLDLIPPVRNSSVAVHAIVYTLVLVSLYITTCVAIKTFFNTD
                     EKLIKRIGVAALNTLVMSVPFINRLSSSSVPDSLGLGIGAGLAYIIAVSLINVGLRYI
                     SRSRTIPEVFKGVPAVLIYVALLSMAFTGISGKPLSL"
     gene            complement(2418..3521)
                     /locus_tag="PLD98_07965"
     CDS             complement(2418..3521)
                     /locus_tag="PLD98_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HQA06215.1"
                     /translation="MNNQDNNVKKGLFYRAFFRNPVLVELVGISSVAAIAVSAETAIY
                     LAIVTMVLLIVAEGITSLILKRLPAWIRIAVYVLIGTLVVTPFVVTMQKVSSTNLNLF
                     LPLAAINSIIVLHCERAAVRHGLKGALRDAFAASFGYGVVAVVTGIIREFLGAGTVFG
                     IDTGAKIYLSGMLMPFGGFLVLGFLAAMLSNNLNKIYGGRKSENAFDMSSVETLSVFA
                     LMLSNAKGISRQFSSLKNRNGARKPVKMKAKQKPERIPDAAAAAPAAETAKEESSGGN
                     ASNKNTENEQSESGRQTAPQERTAPPNPASYERMSRYAGNASTLPIVTTTARANYTSD
                     FDEILKDLSEFNSRKNQNKNDNNTQETDKTGGE"
     gene            complement(3514..>4483)
                     /locus_tag="PLD98_07970"
     CDS             complement(3514..>4483)
                     /locus_tag="PLD98_07970"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HQA06216.1"
                     /translation="DALAMLAAMAVLAWYYYGERALRLILFSALTALLLDWFGNSLLN
                     NKQAADPLYALFTGAAIALLLPASSPYWLPVIGSSFAIIAAKLPFGGKDNLPFVPIAA
                     GIAFLCISFPQMVFSYPAVEQGAEVAVYGSPSFAPSASIAEMLSKNNSIGVNVFGYLN
                     ILTGAIPSPMGTGSLLLMSGIALYMYLRNPKMFISGAGFIATCAIFAVLFPRVITGRR
                     ISLLMELSSGMLLFAAIFLVPQKTLRLKNPTHRLLYGMATGAVCMLLRYFGAYEEGVC
                     FAVMLMNGVIAAMTSPRPVKSRKSPVLTEEKQTEDAVSSERSVAYE"
BASE COUNT         1133 a         1003 c         1169 g         1178 t
ORIGIN      
        1 caggctcagc gaggaccttg tatgccatcg ggagcaggct gtttgttatg gcttttgagt
       61 atctgccgat attaaactga ggatagttta cggggtcgga atatacattg tcaaggtttc
      121 cgataacgaa ctgaaccgca agtcggataa ttgttatgtt cctgcccacc tcatggtgct
      181 gattcttaaa tacccatgtg gtttcgggca tgagagcggt tgaaaggtca atattcttat
      241 cgggtgaaac aaatgacttg tcggtaagcg aatcaatgta atcctgcggc agctgcttgc
      301 cggcgggggc aaaggttgcg ccgagtgttg cggagtcgat atctataatg gcgtctgagc
      361 tgttcttgaa tgtagatttt acagcgccga agaagttgta gtcgccgtca agatatgtca
      421 ggttgtagcc cgcaataaag cctaccttta ctccgtactc gtcccttgct ttaagcaggt
      481 taacggagag attgctctga gcctcatgga aggcgtcggt cttttccttt aacagaacgc
      541 gctcggggtc aaggagatac ttttctgcca gggcgtcgta tctgtcattg ggtatcatag
      601 cccagaactg gggtgagttg actatgagct gaccgaaggc tgcgtcggca gcggctgtaa
      661 tcattgattc aaggactttt ctcggcagga gccttacaag tatgttggca acatagccga
      721 gggcgggtga acctgtaagc tccttaaata cctcgggaag gtactcggag aagaagaact
      781 ggtcctcgag attgaagccc tcatagcctc ttgtaaggaa gtccgcgatt atgtctgtgc
      841 cgctaaggag cgatacgaca ttgatgatgc agttgatgtc gttataatct gcttcctcat
      901 agtttaagta agatgcgaga acggtgccgc ccatgctgac gctggcgatg ttgaccttat
      961 cggtgttctg ctgctccttt accatctgga tgaactcatg cagcttcttt ccgttctcaa
     1021 tgggattgcc aaggagcgga taagagaata catatatgta ctcctcgcct actgcctggg
     1081 cgtaatcctg catgggtatc tgtctgtaga aataatccct gtcctcctcg gagaactcgg
     1141 aaagaggata attgtactca tctattgcga acctgttaac ggttgtgccg tcgggctttg
     1201 atgcctgggt tgagaacagg tcgagtccta actcataaat tgcctgggaa agtcctgcgt
     1261 ccttctgtga aataagggat tttacaagcg ggaagaatac ggtcttaaag attttctgca
     1321 caagacccgc tgtgtcgata ataagaagac cgcctttaag ctcctgaccg tcggcgttaa
     1381 gtatcttttc gccgttttca tcaacaaggt atgaaatggt ctggctgatg cccggaagga
     1441 cgattatggg gtactgctta acgccttctt tgggctgaat cggcagggac tgcgtcttta
     1501 ctaccgtttc gggcttctct tctttatttg cttctgcctc tgcatttaca tctgccgcta
     1561 ctttttcgga ttctgcgcca gcggctgcgg attcctgagc gacagtgctt attttggtag
     1621 aagcaatgtt aaccctgaat gacgaaaaga gaagtgcaat aacaacaatt gcggacaacg
     1681 cttttaatat gcgcttagac atctgtgctc ctcctaacat ctgtgtaaaa cctatatttt
     1741 aatcttttaa ataatagcat attttgtgga tattaacaag caaaaccatt aatttcgatt
     1801 gtaaagaaat tattgacttt ttgttaaagc gacaggggtt ttcccgatat gcccgtgaaa
     1861 gccattgaga ggagcgcaac atagataagc accgcgggga cgcctttaaa gacctctggg
     1921 atagtgcggc ttcttgaaat gtatctgagt cccacattta taaggcttac ggcgataatg
     1981 taggcaagtc ccgcgcctat gccgagaccg agggaatcgg gaacggagga ggatgaaagc
     2041 ctgttaataa acgggacgga cataacaagg gtatttaaag ccgctacgcc tatccttttt
     2101 atcagctttt catctgtatt gaaaaaggtt tttatagcta cacaggttgt aatataaagg
     2161 cttacaagca caagggtgta aacgatagcg tggactgcga cgctggaatt cctgacgggg
     2221 ggaattaaat caagcgtacg gcaaatgaga gccacgagaa cggagaagta gacaataaat
     2281 cccgatgtgt ggaaaagata cttgggcttt gctgccattc tgaccgattc ggttgctccg
     2341 aatccgcccg tgaatatgac attccttata aggcaggtat aaagcgccat gttaatcata
     2401 tcgaaaatag cggacattta ctcccctccc gtcttgtcgg tttcttgagt attgttgtcg
     2461 ttcttgtttt gattctttct tgagttgaac tcggacaaat ccttcagtat ctcgtcaaag
     2521 tcggaggtat agtttgccct tgcggttgtg gtgacaatgg gcagggtgct ggcgttgccc
     2581 gcgtatcttg acattctttc ataagaagct gggttaggag gagcggttct ttcctgagga
     2641 gcagtctgtc tgccgctctc cgactgctca ttttcggtat tcttatttga ggcgttaccg
     2701 ccgcttgatt cttcttttgc tgtttctgcc gcgggagctg ctgcggcggc atcgggaatt
     2761 ctctcgggtt tttgctttgc tttcattttg acgggttttc ttgcgccgtt tctgtttttc
     2821 aggcttgaaa actgtcttga aatgcctttt gcgttggaga gcataagggc aaagacgctg
     2881 agagtctcca cggaggacat atcaaaggcg ttttcgctct ttcttcctcc gtagattttg
     2941 ttaaggttat tgctgagcat tgcggcaagg aagccgagca caaggaagcc gccgaagggc
     3001 atgagcatac ccgaaaggta aatcttggct cccgtatcaa ttccgaaaac cgttcccgca
     3061 cccaaaaact ctcttataat acccgtcaca accgcaacaa cgccgtagcc gaaggaagcg
     3121 gcaaaggcgt cacgcagggc gccttttaag ccgtgcctta cggcagcgcg ctcgcagtgc
     3181 agaactataa tcgagtttat tgcggcaagg ggaaggaaaa gatttaagtt tgtggagctg
     3241 accttctgca tggttaccac aaagggggtt acgacaaggg tgccgataag gacatatacc
     3301 gcaattctaa tccatgcggg gaggcgcttt aaaatcagag aggttatgcc ctccgcgaca
     3361 atcagcagca ccatggtaac tatggcaaga tagatggcgg tttcagcgct gacggcaata
     3421 gccgctacag agctgatacc aaccagctct acaagaacgg ggtttctgaa aaaggctctg
     3481 tagaaaagac ccttttttac attgttgtcc tgattattca taggcaacac tcctttcgga
     3541 gcttacggcg tcttccgttt gcttttcctc tgtcaaaacg ggagatttgc gactttttac
     3601 gggtctggga gaggtcatgg cggcgattac gccgttcata agcattacgg caaagcagac
     3661 gccctcctca taggctccga aataccttaa aagcatacag actgcgcctg ttgccatgcc
     3721 gtaaagcagc ctgtgggtgg ggtttttaag ccttagagtc ttttggggaa caaggaagat
     3781 tgccgcaaac aaaagcattc ccgaagaaag ctccataagc agggaaattc tcctgcccgt
     3841 aatgaccctc gggaacaata cggcgaatat agcgcaggtg gctataaagc ccgcgccgga
     3901 tataaacatt ttgggatttc taagatacat ataaagggcg attccgctca ttaaaagcag
     3961 cgagccggtg cccatgggcg aaggtatagc gcccgttaag atattgagat aaccgaatac
     4021 attgactccg attgagttgt tctttgacag catttcggcg atggacgcgg agggggcaaa
     4081 gctgggactt ccgtaaaccg cgacctctgc gccctgctct acagcgggat aagaaaagac
     4141 catctggggg aatgaaatgc aaaggaaagc tattcccgcg gcaatcggca caaagggcag
     4201 attgtctttg cctccaaagg gcagctttgc ggcgataata gcgaaggagc tgccgataac
     4261 gggcagccag taaggagatg aggcgggcaa aagcagggct attgccgctc ccgtaaacag
     4321 cgcgtaaagg gggtccgccg cctgcttgtt atttaaaagg gaattaccga accaatccag
     4381 caaaagggct gtcaaagctg agaagagaat aagtctcaaa gccctttcac cgtaataata
     4441 ccatgccaaa acggccattg ccgcaagcat ggcaagagca tct
//
LOCUS       DAONVQ010000136         4401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_14156_length_4401_cov_4.327197, whole genome shotgun sequence.
ACCESSION   DAONVQ010000136 DAONVQ010000000
VERSION     DAONVQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14156_length_4401_cov_4.327197"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLD98_07975"
     CDS             <1..213
                     /locus_tag="PLD98_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06217.1"
                     /translation="LADGVKPTGGGKPRMKGKKGGKRPSGKRPAPPRQNRTENASAAE
                     EHRAPIVDLSTAARYERIEIKKEAEM"
     gene            657..1490
                     /locus_tag="PLD98_07980"
     CDS             657..1490
                     /locus_tag="PLD98_07980"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQA06218.1"
                     /translation="MEIINHNFTEDGRVNLKVYIPDKSRELHDFPRPMVLIFPGGGYG
                     FCSDREAEPIALSYNAAGFNAAVLRYSIAEHSGYPAPLADASKAVKFIRDNAERFHTD
                     KDKIAVCGFSAGGHLAAWLGTKWNDKEIQKAAGVENDENKPNALILGYPVISGREPTH
                     NGSINNLLKSVPDEEKESVRNLICCEENVGEHTPPAFIFHTADDAAVPVIGALNFARA
                     LSRNKIPFEIHVYRSGSHGLSLANYVTAKSKNSINADVEGWFDLSVKWILRTFNFPPE
                     F"
     gene            1513..3249
                     /locus_tag="PLD98_07985"
     CDS             1513..3249
                     /locus_tag="PLD98_07985"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HQA06219.1"
                     /translation="MSIKRKLKVALPVAGLTLAAGAIMKQQNICPLCVAKRVLNATRI
                     TYKNEGRFENGVALTPPMGWSSWNLFRHRINEDLIYEIAEAMAKSGLKDAGYTYVNID
                     DCWQSSSRDKNGRLQADLATFPSGIKNLAERVNALGLKLGIYSSNGTLTCEDLPASLY
                     NEAIDADTFAEWGIEYFKYDFCHNKPIPTSAPDIDKIIITKPNEKEGIVIEAETGRLT
                     GKARVAEDSKLETGKYVTGLSANGGALIFDEVEVDEDGEYILTIGLRKAGLYQKYAEI
                     VVNEKDCYDAYFPSTKGFTHEGRHQITIKLNKGVNSIKIHNPVASRMDSAIIQYTKMG
                     KELKRATKEYAERTGQPEKPIVYSICEWGVNRPWKWGAGAGNLWRTTMDIKAFWASIL
                     AIYEFNVRLYKYAGPGNWNDPDMLEVGNGNLTDDENKAHFSLWCMMAAPLILGNDIRT
                     FLKPDGTPDTENKIYKIVTNKDMIAIDQDRRGIQCRRIRTTPSLVDVLVKPLENKELA
                     VCLFNKGEMAAKGEFSIRELTSLGFIDLPKAAVYEVKDIWENEIFTSQDKVSAELPKH
                     SVKVYRIKAITD"
     gene            complement(3294..>4401)
                     /locus_tag="PLD98_07990"
     CDS             complement(3294..>4401)
                     /locus_tag="PLD98_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06220.1"
                     /translation="GIGVIAKNFGGLPTYGWTYIDRTDQCPWLTFTLEDFVDHPALWG
                     NDLMDEPAADSFPYLARMVDSYYSKTNGKIAYINLYPSYANEEQLGTKADWFAFSKDY
                     QRISSYQKHVNAYIETVGTDYISVDIYPLKQRTDKATGKPYKYCEPTWLKNLDILAKA
                     CRDTNRDLWVITQVTGMPFEGDSVTRIADEADIRWQAYTALSFGAKAIIHACYQEGWW
                     DESSHLITRDGQRNPVYFYVQAVNREIKALSDAYSDYKNIGAFVLNAYKESHTRIALS
                     KVEFKAISDLQSEDTLLVGCFEAKEGDSKAFTMVNMNEISKNDRADAKFKLNGASSVT
                     VYRAGVPSELTPDADGFYSLSLAPGEGVFVTVDY"
BASE COUNT         1150 a         1145 c         1135 g          971 t
ORIGIN      
        1 cttgccgacg gagtaaagcc cacaggcggc ggcaagccga ggatgaaggg caaaaagggc
       61 ggcaagcgcc cgtcgggcaa aagacccgct ccgccgaggc agaaccgtac cgagaacgct
      121 tcagcggcag aggagcacag agctccgatt gtggaccttt ccacagccgc aagatacgag
      181 cgcattgaga ttaaaaaaga agctgaaatg taataaaaaa ccgcagccta agagctgcgg
      241 tttttttttt accccgaaac aaaaccgcat agggttttgt ttcggccgga ttgattttgc
      301 ggcaaaacac tttggcggaa gggcgcgctc tttgctcttg ctcttttttt acggcgagct
      361 gcgttcccct ccgccccgaa ccccgtttgc gtcaagggag cggtgttttg cccagagccc
      421 cgtttgcgtc aagggagcgg cgtcttgccc aaagccccgt cttctgatgg aaagcggctt
      481 gccgcctttc tttgctttcc cgctgcgggg caactcccgc aattttttgc gtcgggcagt
      541 cgttcttagg agcttgcctt aatctgatgt ttttattcac cacatttagg ttgaaattgt
      601 cacgctcaaa agctataatg aattaaatgc aaaatttact aaaataaggt gaagatgtgg
      661 aaataataaa tcataatttt accgaagacg gacgagtaaa tctaaaggtt tatatccccg
      721 acaaaagcag ggagctgcac gatttccccc gccccatggt gcttattttc cccggcggcg
      781 gctacggctt ctgctccgac agagaggctg agcccatcgc tctctcatac aacgccgcgg
      841 gctttaacgc cgctgtgctc aggtactcca tagcggagca ttcaggctat cccgcccccc
      901 ttgcggacgc ttctaaggct gtcaagttta taagggacaa cgcggagaga ttccacaccg
      961 acaaggacaa aatcgccgtt tgcggcttct cagcgggcgg tcaccttgcc gcgtggctgg
     1021 gcaccaagtg gaacgacaaa gagattcaaa aggccgcggg cgttgaaaac gacgagaaca
     1081 aacccaacgc gctgattctg ggctaccccg taatcagcgg cagggagcct acccacaatg
     1141 gctcaatcaa caatctttta aagtccgttc cagatgaaga aaaagagtct gtccgaaatc
     1201 tcatctgctg cgaagaaaat gtaggcgagc acaccccgcc cgcgtttatc ttccacaccg
     1261 cggacgacgc cgctgttccc gtaatcggag ctctcaactt cgcgagagcg ctcagccgga
     1321 acaaaatccc ctttgaaatc catgtttaca gaagcggctc acatggtctg tctctggcta
     1381 actatgtaac cgcaaaatcc aaaaacagca tcaatgccga tgttgaaggc tggtttgatt
     1441 tatcggttaa gtggatactg aggacattca attttccacc ggaattttag ataaaagaaa
     1501 ggacgggatt tgatgagcat taaacgcaag ctgaaagtcg ctctgcccgt agcgggtctt
     1561 accttagcgg caggcgcgat tatgaaacag cagaacatct gcccactctg cgttgccaag
     1621 cgagtactca acgccacccg cataacctac aagaacgagg gcaggtttga aaacggcgtg
     1681 gcactcactc cgcccatggg ctggtcaagc tggaaccttt tcagacacag gattaacgag
     1741 gacctgattt acgaaatcgc cgaggctatg gcaaaatcgg gacttaaaga cgcgggctat
     1801 acctatgtaa atatcgacga ctgctggcag tcctcctcaa gggacaagaa cggcagactt
     1861 caggccgacc ttgccacctt cccgtcgggc atcaaaaacc ttgccgaaag ggtaaacgct
     1921 ctgggcctga aactcggcat ttactcctcc aacggcactc tcacctgcga ggacctgccc
     1981 gcaagccttt acaacgaggc tatagacgcg gacacctttg ccgagtgggg cattgagtac
     2041 ttcaaatacg acttctgcca caacaagccc atccccacca gcgcccccga cattgacaaa
     2101 atcatcatta caaagcccaa tgaaaaagag ggcattgtaa tagaggcgga gacaggcagg
     2161 ctcacaggca aagcaagggt cgccgaggac tccaagctgg aaactggcaa gtatgtaacg
     2221 ggactttccg caaacggcgg cgcacttatc tttgacgagg tggaagttga cgaggacggc
     2281 gagtatatcc tcacaatagg tctcagaaaa gcgggacttt atcagaagta cgccgaaatc
     2341 gtggtcaacg aaaaagactg ctacgacgca tacttcccgt caaccaaggg ctttacccat
     2401 gagggaagac accagattac aattaagctc aataaaggcg taaacagcat taagatacac
     2461 aatcccgtag cctcaagaat ggacagcgcc attatccagt acaccaagat gggcaaggag
     2521 ctcaagaggg caacaaaaga gtacgccgag agaacgggac agcccgaaaa gccgattgta
     2581 tattcaatct gcgagtgggg cgtaaaccgt ccgtggaagt ggggagctgg cgctggcaac
     2641 ctgtggcgca ccacaatgga catcaaggca ttctgggcat caatcctcgc aatttacgag
     2701 ttcaatgtaa ggctttacaa gtatgccggt cccggcaact ggaacgaccc cgatatgctt
     2761 gaggtgggca acggcaacct gacagacgat gagaacaagg ctcacttcag cctctggtgc
     2821 atgatggcag ctccccttat tctgggcaac gatatcagaa ctttcttaaa gcccgacggc
     2881 acccccgaca ccgagaacaa gatttacaag atagtcacga ataaagacat gattgccatc
     2941 gaccaggaca gaaggggcat acagtgccgc agaatcagga ccaccccgtc ccttgtggat
     3001 gtgcttgtaa agccccttga gaataaggag ctggcggtct gcctctttaa caagggcgag
     3061 atggccgcaa agggcgagtt ctccattcgg gagctgacct ccttaggctt tatcgacctg
     3121 cccaaggctg ccgtatatga agtcaaggac atttgggaga acgaaatctt cacaagtcag
     3181 gataaagtct ccgcggagct gcccaagcac agcgtaaagg tctacagaat caaggcaatc
     3241 accgattaat aaaaaaagcc gtaccaaaag gtacggcttt ttttattgaa gatttaataa
     3301 tctaccgtta caaacacgcc ttcgccgggg gcaagggaga ggctgtagaa gccgtcggcg
     3361 tcgggagtaa gctctgaggg aactcccgcc ctgtaaacgg ttacggagct tgcgccgttc
     3421 agcttgaact tagcgtcggc tctgtcgttc ttgctgattt cgttcatgtt gaccattgta
     3481 aatgccttag agtcgccctc tttagcctca aagcagccca caagcaaggt gtcctcactc
     3541 tgaaggtctg atatagcttt aaactctacc ttagaaaggg ctatgcgtgt atggctctct
     3601 ttatatgcgt taagcacaaa cgcgccgatg ttcttatagt cggaatatgc atcggatagt
     3661 gcctttatct ctcggttcac agcctgaaca tagaagtaga cgggatttct ctgaccgtca
     3721 cgggtgatga ggtgagagct ctcatcccac catccctcct gatagcaggc gtgaattatg
     3781 gcctttgcgc cgaaagagag cgcggtgtat gcctgccagc ggatgtcagc ctcgtcggct
     3841 attcttgtaa cgctgtcgcc ttcaaagggc atacccgtta cctgagtgat tacccaaagg
     3901 tccctgtttg tatctctgca tgccttggcg agaatgtcaa ggttcttaag ccaagtgggc
     3961 tcgcagtatt tgtagggctt gcccgttgcc ttgtcggtgc gctgctttaa ggggtagatg
     4021 tcaacgctta tgtagtcggt tccgacggtt tcgatatacg cgttaacatg cttttgatag
     4081 gagctgattc tctgataatc ctttgagaaa gcgaaccagt cggctttggt gcccagttgc
     4141 tcctcatttg cgtaggaggg ataaaggttt atgtatgcta tcttgccgtt tgtctttgaa
     4201 tagtagctgt caaccatcct tgccaggtag gggaaggaat cagcggcggg ctcgtccatc
     4261 aaatcgttgc cccagagagc gggatggtcc acaaaatcct caagggtaaa ggtcagccac
     4321 gggcactggt ccgtcctgtc aatataagtc cagccgtagg tgggcagacc gccgaagttc
     4381 tttgctataa cgcctatgcc g
//
LOCUS       DAONVQ010000137         4342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_14370_length_4342_cov_4.261022, whole genome shotgun sequence.
ACCESSION   DAONVQ010000137 DAONVQ010000000
VERSION     DAONVQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14370_length_4342_cov_4.261022"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLD98_07995"
     CDS             <1..400
                     /locus_tag="PLD98_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQA06221.1"
                     /translation="LLFETVVVFDEPKCDIEIPFDEENLDSLNFLSGKTLSYVNRRAF
                     EATVLAHTDGAVPNIVIECGKMDEYNLGCLIYFFEKACAMSGYMLGVNPFDQPGVEDY
                     KRNMFALLEKPGYEELSKALLDRIKSECAL"
     gene            515..931
                     /locus_tag="PLD98_08000"
     CDS             515..931
                     /locus_tag="PLD98_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06222.1"
                     /translation="MISLIFGPKGSGKTKKLLDLLNIAIESSEGNVVCVEKTNALSHQ
                     VNYRARLVLADDYDIEGFDAFYGFLGGLCAGDHDITDIFVDATLRIGSRDMNELAAFL
                     KKVAKLSEKTETDFVFTVSADPATVPDEILKSYKIL"
     gene            1046..2512
                     /locus_tag="PLD98_08005"
     CDS             1046..2512
                     /locus_tag="PLD98_08005"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HQA06223.1"
                     /translation="MENNLPISGSASLALDFHEILMSSAFFSSGLDKTVVCYDLFYRP
                     APETGGFCIMAGLEQIVKNILNFKPSESDLEWLRQKGVSSEFIDFLKNFKFSCDIYSV
                     PEGTPVFPGEPIIMVKGPAAEAKLIESLLISAVGKQTLIATKANRLARAAHGRKVVEC
                     GAKRESGFDASLYASRAAYIGGCCGTTNTAAAKEFNIPLYGNMTNSWIQMFDNELDAF
                     CAYARAYPDDCVLLIDTYDVLNSGIKNAIEAFRREILPKGKRPKAVRIDSGDLAYLSK
                     RVRRILNDEGFPDCDIIVSNALDEHIIKDMLQNGARVDCFMVGSRLISSSSAPSLPIV
                     YKLVAVENNGRLVPKIKISENVSKITNPGAKTFWRFFDRETGKAIADLLSLEHEIINE
                     KEPYELFDPDFVWKRKIVQNFVARKMLVPIFKKGELVYTLPNASEVRAYCADQIDSLW
                     EEVLRFENPHKYYVDLSQELWDLKRSLISKYSGVKFAD"
     gene            2526..3737
                     /locus_tag="PLD98_08010"
     CDS             2526..3737
                     /locus_tag="PLD98_08010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HQA06224.1"
                     /translation="MNKKRKRRLSYLPIYILTFVLTTSVAFLSTYNDSKDVANLTSPP
                     SETEEATTAQPRQEILRASFLAVGDNLIHSKLYEQAKERAGGSGYDFKYLYQNVAEDI
                     KNADIASINQETIIAPSKPPSTYPLFNSPPELGKEVVRLGFDIINIANNHMLDKGSSG
                     LKEAIEFWRSQDGVKMTGAYLNESEMYVPETITRNGIVFGIVGITDTTNGLSLPANSE
                     LRYFSAAETDKIRRKIELTKEACDVVVVNIHWGNEYSTKPSENQKFLAKKLIEWGADI
                     VIGHHPHVLQPIELIRKIDGGTAVVAYSLGNFVSMQNTGPTLIGGMLRFNVIVDKSSK
                     YVTVEDVKLEPLVMHHKKSYLNGRLYKLSQYNDSLARESTATIKTPAFSMDYIKKHVK
                     GIISEEFLDIS"
     gene            4053..>4342
                     /locus_tag="PLD98_08015"
     CDS             4053..>4342
                     /locus_tag="PLD98_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQA06225.1"
                     /translation="MLRDSAKNREMFSDYLNDSSVKIVWGDLCDFDSVLKCVTGADIV
                     LHVGGLVSPAADYFPLKTQEVNVKAMQNIVDAVKAQPDPDSIKVVYIGTVAQ"
BASE COUNT         1249 a          969 c         1017 g         1107 t
ORIGIN      
        1 gcttctcttt gaaaccgtag tggtattcga tgagcccaag tgcgatattg aaataccctt
       61 tgatgaggaa aaccttgatt cacttaattt cctctccggc aagaccctct cttatgtaaa
      121 ccgcagagct tttgaggcaa cggttcttgc ccataccgac ggcgctgttc cgaatatcgt
      181 aattgaatgc ggcaagatgg acgagtacaa tctcggctgc cttatctatt tctttgaaaa
      241 ggcatgcgcc atgtcgggct atatgctggg cgtaaatccc tttgaccagc cgggtgttga
      301 agattacaag agaaatatgt tcgctctcct tgaaaagccg ggctatgaag agctgtcaaa
      361 agcgcttctt gacagaatca aatccgaatg cgccctttag gactttacga gaaaggcaga
      421 gggcggtctt gaactaagat tggcggcagc aataatagcc gaaaagcctt tgcctttccg
      481 ttgttaatat ttttacgaaa gggagtagat taaaatgata agtttaattt ttggtcccaa
      541 aggcagcggt aagaccaaaa aactgctgga cctccttaac atcgctattg aatccagcga
      601 gggcaatgtt gtctgcgttg aaaagacaaa tgctcttagc catcaagtga attatcgcgc
      661 cagattagtc cttgccgatg attacgatat tgaaggcttt gacgctttct acggcttcct
      721 cggcggactt tgcgccggcg atcatgatat aaccgatatt tttgtagacg ctactttaag
      781 aatcggctct cgtgatatga acgagctcgc agccttcctc aaaaaggttg caaagctgtc
      841 ggagaaaacc gaaaccgact ttgtattcac ggtttccgca gaccccgcaa cggttcccga
      901 cgagatttta aagagctaca agattctgta acgctgtaac ataagctttg ctgtgcctgc
      961 ccaaaaggcg ggtacagcaa agtaagtgtt ttttgggcgg ttaaaattat ttgttaagcg
     1021 ccgaatccaa aaaggggatg tatttatgga gaataatctg cctattagcg gctctgcttc
     1081 tcttgctttg gattttcatg aaatactgat gtcaagcgcc tttttctcaa gcggtcttga
     1141 caaaaccgtt gtctgctatg atttgttcta caggccggct cccgaaacag gcggcttctg
     1201 cattatggcg ggacttgagc agatagtaaa gaatattctg aactttaagc cctccgagtc
     1261 cgaccttgaa tggctgagac aaaagggcgt ttcctctgaa tttattgatt tccttaaaaa
     1321 ctttaaattc agctgcgaca tttattccgt gcccgagggt acgcctgttt tcccgggcga
     1381 acccataatc atggtaaagg gccccgccgc tgaggcaaag ctaatagagt ccctgcttat
     1441 ttccgccgtc ggcaagcaaa cgctgattgc caccaaggca aacaggcttg caagggcagc
     1501 ccacggcaga aaggtagttg agtgcggagc aaagcgtgag agcggatttg atgcgtctct
     1561 gtacgcttca agggcagctt atatcggtgg ctgctgcggc accaccaaca ccgccgccgc
     1621 aaaggagttt aacatccccc tttacggcaa catgacaaac agctggattc agatgtttga
     1681 caatgagctt gacgctttct gcgcgtacgc aagggcatat cccgacgact gcgtactgct
     1741 tattgatacc tatgatgttt tgaattccgg cattaagaat gccatagagg catttagacg
     1801 cgagattctc cccaaaggca aaagacccaa ggctgtaaga atagacagcg gcgaccttgc
     1861 ctatttgtct aagagagtaa ggcgcatact caacgacgaa ggcttcccgg attgcgacat
     1921 tattgtttcc aacgctttgg acgagcatat tatcaaggat atgctccaga acggcgcgag
     1981 agttgactgc tttatggtag gctcaaggct tatttcttca tcctcagccc cgtcgctccc
     2041 gatagtttat aagctggtcg ctgtagagaa taacggacgc cttgtgccca aaatcaaaat
     2101 ctccgaaaat gtttcgaaaa tcacaaaccc cggagcaaaa actttctgga gattttttga
     2161 cagggaaaca ggcaaggcga ttgcggattt actcagcctt gaacatgaaa tcataaacga
     2221 aaaagagccc tatgagctgt ttgaccccga ttttgtctgg aaacgcaaaa tagttcagaa
     2281 tttcgtcgca agaaaaatgc ttgttcccat tttcaaaaag ggcgagcttg tatatacact
     2341 tcccaatgca tcggaagtaa gagcatactg cgccgaccag atagactcgc tttgggaaga
     2401 agttctcagg ttcgaaaatc ctcacaaata ctatgtcgat ttgtcccagg agctgtggga
     2461 tttaaagcgg agcttaattt caaaatacag cggggtcaaa tttgctgatt aaaggatgtt
     2521 gaagaatgaa taaaaaaagg aaaaggcggc taagttatct tccaatatac atcttaacct
     2581 ttgtgctgac tacctcggtc gcctttttga gcacctacaa cgactccaag gatgtggcaa
     2641 acctcacaag cccgccctcg gaaaccgaag aggcaacaac tgctcagcct cgtcaggaga
     2701 ttctcagagc ctcatttttg gctgtgggcg acaacctgat tcattccaag ctctatgagc
     2761 aggcaaagga gagggcaggc ggctccggct atgattttaa atacctctat cagaatgtgg
     2821 cggaggatat taaaaacgcc gatattgcct caataaatca ggagacaatc atcgctccca
     2881 gcaaaccgcc ctcaacttac ccgctgttta attccccgcc ggagctgggc aaagaggttg
     2941 taaggctcgg ctttgacatt ataaacattg ccaacaacca catgcttgac aaaggctcat
     3001 cgggacttaa agaggctatc gagttctggc gttctcaaga cggcgttaaa atgacgggcg
     3061 cgtatctgaa tgagagcgaa atgtatgtcc ccgaaaccat aacccgaaac ggaattgttt
     3121 tcggaatcgt gggaattacc gataccacaa acggattgtc gctgcccgct aactctgagc
     3181 tgagatactt ctccgccgcc gaaaccgaca agattcggag aaagattgag ctcaccaaag
     3241 aagcgtgtga tgtggtggtt gtaaacatcc actggggtaa tgaatactca acaaagccgt
     3301 cggaaaatca aaaattcctc gcgaaaaagc tgattgagtg gggcgcggat attgttatcg
     3361 ggcatcatcc ccatgtgctg cagcccatag agcttataag aaagattgac ggcggcaccg
     3421 cggttgtggc atactcttta ggcaatttcg tttcgatgca gaacacaggt ccaacgctga
     3481 ttggcggcat gctcagattt aatgtaattg tggacaaatc ctcaaaatat gttactgttg
     3541 aggatgttaa acttgagccc cttgtaatgc accacaaaaa gagctacctg aacggcagac
     3601 tttataagct gtcccagtac aatgacagcc ttgcaaggga gagcacagca acaatcaaaa
     3661 cccccgcgtt cagcatggac tatataaaaa agcatgtaaa aggcattatt tcagaggaat
     3721 ttctcgatat aagctaaatg ttaataaaat ctgaattttg aaaaaagttg accattcgac
     3781 aatggtcaat ttttttattt tttctgaaaa aattggagtt cttgaatagc cctaaagcta
     3841 tagaattatc tgggaatata aggtactatt aaaatcagtt ttttaataaa cgggcaaagt
     3901 ataaacgagt aattacggtt ttggattcag taccccaaag gagtaaagac catggctaag
     3961 aaaacagtat ttttaacagg cgcatcggga aatatgggct ttgagggctt taagcagctc
     4021 tatcaaaaca aaaagagttt aacatcgtcc ttttgttaag agacagcgca aagaacaggg
     4081 agatgttctc tgactaccta aacgacagca gtgtaaagat tgtatggggc gacctttgcg
     4141 attttgattc ggttctcaaa tgcgtaacgg gcgccgatat cgttctgcat gtcggcggat
     4201 tggtttctcc cgcggctgac tatttcccct taaagactca ggaagtcaat gtaaaggcta
     4261 tgcagaatat cgttgacgcg gtcaaggctc agcccgaccc tgacagcatc aaagtagtat
     4321 atataggcac cgtcgcccag at
//
LOCUS       DAONVQ010000138         4098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_15403_length_4098_cov_4.675241, whole genome shotgun sequence.
ACCESSION   DAONVQ010000138 DAONVQ010000000
VERSION     DAONVQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15403_length_4098_cov_4.675241"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLD98_08020"
     CDS             complement(<1..584)
                     /locus_tag="PLD98_08020"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HQA06226.1"
                     /translation="MGSVVGIFWYGSTVKRSNNRGTVFGTYDIGGLVGFNCATVKYSY
                     NTGTVSGTDFVGGIVGYNGDSSVVTQCYNIGSISGSEFVGGVAGNNSFSKELSESFNA
                     GPVSGNAVIGGVAGSNSWYSSMVNCYNIGRVWGTESVGGVVGEVSYYATVENCYYLNT
                     CTEISSEFGTALTEAQMQETTDFIGFDFESVWML"
     gene            complement(618..1478)
                     /locus_tag="PLD98_08025"
     CDS             complement(618..1478)
                     /locus_tag="PLD98_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HQA06227.1"
                     /translation="MNFMPISALSAEEVWDGTFPAADENAAYSGGSGLQEDPYLISTA
                     EDLAQLSVNTNEIPDYTVDKYFKQTADIILNSPDVFARDEKGYITGIAEGKTANAWTP
                     IGNHFYFTNSFCGNYDGAGHAVRGIYIETEVDYQGLFGYINNGSVSRLGIEDSFIFGW
                     SWVGGVAGYHSQFSTITDCHNNGTVKGFWGIGGIVGENGGTVTQSYNTGYVWGNERTG
                     GVVGYNTHSAIVIDSYNTGMVSGYDYVGGIVGYNGSSAQVKNSYYSGAVSGEAYVGGV
                     IGRDYGNVKK"
     gene            1811..3409
                     /locus_tag="PLD98_08030"
     CDS             1811..3409
                     /locus_tag="PLD98_08030"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HQA06228.1"
                     /translation="MDLGHSISKLRTDANLTQEKFAQILNVSRQTVQKWEYGEALPDI
                     HNIIKIAKFFNISLDALVLRSDGRIVEELSYDKKMQPAYEKIHDWESYSSQLICEYRE
                     SVEEGKDIEKYKDLINAVSKMPASRHKEQIADVIFDIILNSPQAEEYKYNEPSDLEHI
                     KLLRPEFHCASQKALSADELESKIHGAWLGRISGCLLGKPIEGIKTSELHPILKESGN
                     FPMRRYIKSTDITEEMCEKFSFRLKDRCYADTISFAPVDDDTNYTVLAQVLIEKYGRD
                     FTPYDVSRVWTDHQPKLAYCTAERVAFRNFVYGYVPPYSAVYKNPFREFIGAQIRGDY
                     FGYINPGNPELAAEMAWRDASISHTKNGIYGEMFVAAMIACAAVCDNIEDVIMVGLAQ
                     IPSTSRLYEAVTKVMDDYKNGVSEQECFAKIHQQYNENDPFDWCHTIPNAVIVTAALL
                     YGKGDFSKSVCLAVQTGFDTDCNGATVGSVVGIMNGAESIGEEWTKPFNGELETSVFG
                     MSMVKVTDLVATTLKHIEKGSQKA"
     gene            3574..3864
                     /locus_tag="PLD98_08035"
     CDS             3574..3864
                     /locus_tag="PLD98_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06229.1"
                     /translation="MDNIALYIDLTTGGFIEPSDNVLFNNVRFTNGDISYNAGTGTIS
                     LNRAGRYSIQWWVDTLTGGSASGLQFTLASVGASYPSHLHQQNRSNKRLCDY"
BASE COUNT         1172 a          971 c          879 g         1076 t
ORIGIN      
        1 tcaagcatcc aaacactctc aaagtcaaaa cctataaagt ccgtagtttc ctgcatttga
       61 gcctctgtaa gagctgtgcc gaattcactg gaaatctctg tgcatgtgtt aaggtaatag
      121 cagttttcca cagtggcgta ataggagacc tctccaacca caccgcctac actctcagtt
      181 ccccaaaccc tgccgatgtt atagcagttt accatggatg agtaccatga gttggaacct
      241 gccactccgc cgattactgc attgcccgaa accggacctg cgttaaagct ttccgacaat
      301 tctttgctga aactgttgtt tccggccacg ccgccgacaa attcgcttcc cgaaatcgag
      361 ccaatgttat agcactgcgt tactacggaa gaatcaccgt tatatcctac aatgccgccg
      421 acaaaatcag tgccggaaac cgtgcctgtg ttatagctgt attttactgt ggcgcagtta
      481 aaaccaacca atccgcctat atcataagtt ccgaaaaccg tgcctctgtt attgcttctc
      541 ttaacagttg agccgtacca gaaaattccg accacgctgc ccacatatgt ttcgcccgaa
      601 acagcgcctg tattacttca ttttttaaca ttgccatagt cgcgtcctat tacgccgccg
      661 acataagcct ctcccgaaac agcgccgctg taatagctgt tttttacttg agcggatgag
      721 ccgttatatc caacaatacc acccacataa tcataacctg aaaccatgcc tgtgttatag
      781 ctgtctatga ctattgcgga gtgcgtgtta tatccgacca cgccgcctgt tcgttcgttt
      841 ccccaaacat agcctgtgtt atagctttga gtcactgtgc cgccgttttc tccaactatg
      901 ccgccgatac cccaaaatcc tttaaccgtg ccgttgttat ggcagtcggt tattgttgag
      961 aactgagagt gatatccggc cacgccgccg acccagctcc agccgaaaat aaatgaatct
     1021 tcaattccaa ggcgtgatac tgagccatta ttgatataac cgaacaaacc ctgataatca
     1081 acttctgttt cgatatatat accgcgaacc gcatgacccg ctccgtcata gttgccgcaa
     1141 aaagaatttg taaaataaaa atgattgccg atgggtgtcc aagcatttgc cgttttgccc
     1201 tcggcaatcc ctgtaatata acctttttca tcacgcgcaa acacatccgg actattaaga
     1261 ataatgtccg cagtctgctt aaaatattta tcaacggtgt agtcagggat ttcgtttgta
     1321 ttaaccgaaa gctgcgccaa gtcttcggca gtggaaatca gatacggatc ttcttgtaag
     1381 cccgaaccgc ctgaatacgc ggcgttttca tctgccgcag gaaaagtgcc gtcccaaact
     1441 tcttccgccg ataatgcgga aataggcatg aagttcaata gcaaaaaata gccagcaagg
     1501 ttgacaatac tttttttgtt tttaccacga caataccctc cctcaacaaa ataatctcta
     1561 aagcaacatt atgaaaaaat attgacatat ttttccgtga atttcaataa cagaaataaa
     1621 ttgtaaaaac ttttgttgct tatctttaaa gaaaccatta ttttgagctc gctgatatcc
     1681 acatactgtt cttcattgca tcaaatgcaa actataaatg gttgcaaaaa gcttatgtaa
     1741 atagcataat taaaaaggat tattattcgt gccgctatca cgctttttat atgtgaaagg
     1801 attgaaaaac atggatttag gccattcaat ttcaaagctt aggacggacg ctaatttgac
     1861 tcaggagaaa ttcgcgcaga tacttaatgt atcccgacag acggtgcaga aatgggaata
     1921 cggcgaggcg ctgcccgata tacataacat tataaaaatc gcgaaattct ttaatatctc
     1981 attggatgcg ctggttttaa gaagcgacgg gcggatagtc gaagagctgt catatgacaa
     2041 aaaaatgcag cctgcctatg aaaagattca tgactgggag tcatattctt cacagctgat
     2101 atgcgaatac agggaatccg tcgaagaagg caaggatatt gagaaataca aagatttgat
     2161 taacgctgtt tccaaaatgc ccgcaagcag gcataaggag cagattgcgg atgtaatttt
     2221 tgatataatt cttaacagcc ctcaggcaga agagtataaa tacaacgagc cttcggattt
     2281 ggagcatata aagcttttga gacctgagtt ccattgcgct tcgcaaaaag ctttaagcgc
     2341 ggacgaactg gaaagcaaaa tccacggagc gtggcttggc aggatttcgg gctgcctttt
     2401 gggaaagccc attgaaggaa ttaaaacgag cgaactccat ccgattttaa aagaatcggg
     2461 caatttcccc atgcgcagat atatcaaatc taccgatatc acagaggaaa tgtgcgaaaa
     2521 gttctcgttc agattaaaag acaggtgcta tgccgatacc atatctttcg ctcccgttga
     2581 cgatgacacc aattacactg tattggctca ggtgcttatc gaaaaatacg gacgggactt
     2641 cactccctat gatgtttcaa gggtctggac ggaccatcag cccaaactcg cctactgcac
     2701 ggcagagcgt gtggcattcc gcaattttgt ctatggctat gttcctccgt attccgctgt
     2761 gtataagaat ccgttccgtg agttcatcgg agcacagatt cgcggcgatt atttcggata
     2821 tataaatccc ggaaatcccg aattggcggc ggaaatggca tggcgtgacg cttcaatctc
     2881 ccacaccaaa aacggaatct acggcgaaat gtttgtggcg gcaatgattg cctgcgctgc
     2941 cgtatgcgac aatattgagg atgtaattat ggttggactt gcccagatac cttcgacctc
     3001 caggctttat gaagcggtca caaaagttat ggacgactat aaaaacggcg tcagcgagca
     3061 ggagtgcttt gcgaaaatcc atcagcagta caacgaaaac gacccctttg actggtgcca
     3121 taccatcccg aacgcggtta tcgtcacggc ggcgttattg tacggaaaag gagatttctc
     3181 aaagtctgtt tgccttgcgg ttcagacagg ctttgacacc gactgcaacg gagcgacggt
     3241 cggctctgtt gtgggaataa tgaacggcgc tgagagcatc ggagaggaat ggacaaaacc
     3301 ctttaacgga gagctggaaa catcggtttt cggcatgagt atggtgaagg tcacggattt
     3361 ggtggcgaca accttaaaac atattgaaaa aggctcgcaa aaggcataaa agctatatag
     3421 taagttttag tccgaggagt aatactcctc ggacttcttt gatttaataa tcagttcaaa
     3481 cagaaccctt aacggaaaag gccatacaat gatatagacg agccttgcgg ctttcagctt
     3541 gatttagcac ttttcttttt aaggagagtt aatatggata atattgcact gtatatagat
     3601 cttaccacag gcggatttat tgaaccgtct gataatgtac tgtttaacaa tgtaagattc
     3661 accaacggag acataagcta caatgccggc acgggtacaa tcagcttaaa ccgcgcgggc
     3721 agatactcaa ttcagtggtg ggttgacact ctgacaggag gctccgcaag cggccttcag
     3781 tttacgcttg cctccgtcgg agcttcctat ccatcccacc tccatcagca aaacaggtca
     3841 aataagcggc tttgcgatta ttaatgtgaa tatcccgccc accacggttt ttttaagaaa
     3901 cgacagcccc ggcagagtgt attattctcc caccgcaaat gttaaagcgt ccttgatggt
     3961 aacggcgcac gctcccgctt tcggctccgt tctgacggac gcttccagtg tttcgcttag
     4021 caatacagcg actactttga cattggattt acagtcaagc gattcttccg gcgtggattt
     4081 catgacaaac cccaacgc
//
LOCUS       DAONVQ010000139         4097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_15409_length_4097_cov_4.151905, whole genome shotgun sequence.
ACCESSION   DAONVQ010000139 DAONVQ010000000
VERSION     DAONVQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15409_length_4097_cov_4.151905"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLD98_08040"
     CDS             <1..234
                     /locus_tag="PLD98_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06230.1"
                     /translation="ADLNNPDELDESMLTECVETGVCKYFDMPNENPDIEEFLRSYGA
                     YEDSGDAESKSSDDKETEAEKEPALTAEGGQAQ"
     gene            231..1145
                     /gene="ftsX"
                     /locus_tag="PLD98_08045"
     CDS             231..1145
                     /gene="ftsX"
                     /locus_tag="PLD98_08045"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HQA06231.1"
                     /translation="MKHASIRYLTKEGFRNIWSNRLMSLASVSVLSACLVLLGCSFML
                     FANIDAVLGRIEAQNVIMVFAKDDATDAELDELKNAIDSTPNVAYSEFIPKEQAYQEL
                     LESLGEDASLLEGLGNDILPDAFRVTVSDMNQYDNTVEALSSLDNILRIRRNSDLATR
                     LIGMRTSIVYIGAGIIIMLLFVALFIIANTVRITMFTRRLEISIMKAVGATNSFIRWP
                     FMVEGILLGIISASLSVGLVFLIYRLAATHIQNLIGILGATLVPFGEYAVMLYLGFLA
                     VGILTGFFGSLVSLGKYLREQGSVVKDE"
     gene            1180..2481
                     /locus_tag="PLD98_08050"
     CDS             1180..2481
                     /locus_tag="PLD98_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQA06232.1"
                     /translation="MAVLCAVMAAVVLFSALSPAVYAQTDEERIKELQRQADELQKKI
                     DAANEQLKKIAGDKAQEQKYINELNAQIEALQKQINAYNSSIDIIDSQIKSLDNQIIE
                     TKSKISKKEAEKQKLEEEVAALEGEIDDTYLKLKERLRAIYMNGTTSDWEILLSGKDL
                     SMYFLRTELTEGIAKHDNQLIAGLKERISALQSKKEQIKKEIDEINEEQQKLKQHQDT
                     LDEKKDALNTQKKKVSSSKSTINTKLEESTKLLRTLNKESETYKKLVKKYEQDKAEFE
                     AEINRILNNESSSGSGSIVGGGKMIWPVPYSNCYITSPYGYRNDPFTGKWTGHGGVDI
                     SIRGGAYGKNIVAVLDGTVVIATYHYSYGNYLAIDHGNGLVTIYAHCSKLLVGKGDRV
                     KQGQVIALIGSTGSSTGPHLHFEVRVNNVRQDPMKGYISLP"
     gene            2505..3713
                     /locus_tag="PLD98_08055"
     CDS             2505..3713
                     /locus_tag="PLD98_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQA06233.1"
                     /translation="MSKKISLGLALAITFVSIAAAIAITMTTSMKIYNNLIKDLPGRS
                     QMYAKLAEIDDLVRSEFYGKIDEEVLEDLLAQGYIAGTGDPFSRYMTADEYISYYNEI
                     AGKMTGIGIRPSLDRDTGYILVQEVYGDSPAAAAGISKGDMITAIDGVKVEASNYREM
                     VSKLEGDKLTSVSVTFIQSETGQKLTANISKGFTSRSVSYEKLGSIGYIRITDFYKNT
                     ASQFKDAVERLKSESVTGLIIDVRNNSKGEIRYAAEVIDVLVPLATEGTGAIATAINA
                     AGEVIEVYSADSENVNLPMVVLVNKYSEGGAELLACDLRDYGKAQIVGETTGGKGTLQ
                     QVFRLDDGSAVLLTVAEIKPYISESFNGVGVTPDFPVELTLSNPEDLWLLDREKDPQF
                     QKAYEILTEE"
     gene            3799..4005
                     /gene="rpmE"
                     /locus_tag="PLD98_08060"
     CDS             3799..4005
                     /gene="rpmE"
                     /locus_tag="PLD98_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQA06234.1"
                     /translation="MKKNIHPKYQPVTVKCACGETFETSSTKEDLRVDICSKCHPFFT
                     GKQKLVDTGGRVDRFNKRFNINAD"
BASE COUNT         1227 a          979 c          994 g          897 t
ORIGIN      
        1 gccgacctta ataatcccga tgagcttgat gaaagcatgc ttaccgagtg cgtggaaacg
       61 ggcgtctgca agtattttga tatgccgaac gaaaaccccg atatagagga gtttttaaga
      121 agttacggcg cgtatgaaga tagcggcgac gccgagagca aatcctccga cgacaaagaa
      181 accgaggcgg aaaaagaacc cgctctgact gccgaaggag gacaagccca atgaaacacg
      241 caagcattag atacttaacc aaagagggct ttcgcaatat ctggagcaac cgccttatgt
      301 cccttgcctc ggtgtccgtt ctttccgcat gccttgtgct tttgggctgc agctttatgc
      361 tctttgcgaa tattgacgcg gttcttggca gaatcgaagc tcaaaatgtt ataatggtct
      421 ttgcaaagga cgacgcaact gacgcagaac tcgatgagct caaaaacgct atcgactcaa
      481 cgccgaatgt ggcgtacagc gagtttatcc cgaaagagca ggcgtatcag gagctcctag
      541 aatctttggg cgaggacgcc tcgctccttg aaggtctcgg caacgacatt cttcccgacg
      601 ccttcagagt caccgtgagc gacatgaacc agtatgacaa tactgttgag gcgctaagct
      661 cccttgacaa tatccttaga atcaggcgca acagcgacct tgccacaaga cttataggca
      721 tgcgcacctc gattgtttac ataggcgccg gcattattat aatgctccta ttcgtggctc
      781 tgttcatcat agcaaacaca gtcagaatca caatgtttac ccgcaggctt gaaatcagta
      841 taatgaaggc ggtaggcgcc acaaatagct ttatcaggtg gccctttatg gtggagggca
      901 tactgctcgg aattatttca gccagcctaa gtgtagggct cgttttcctt atatacagac
      961 tggctgcgac acatatacag aaccttatag gaattctcgg cgcaacgctc gtccccttcg
     1021 gagagtatgc cgtaatgctt tacctcggct tcctcgctgt gggaatcctt acaggtttct
     1081 ttggaagcct tgtttcactc ggaaaatatt taagggaaca gggaagtgtg gttaaggatg
     1141 aataaaagag aagggataca tattttaaaa ggaaaggtta tggcagttct gtgcgccgta
     1201 atggcggcag tggtgctctt ttccgccctc agtcccgcgg tctacgccca gaccgatgag
     1261 gagaggataa aggagctgca gcgtcaggcg gatgagcttc aaaagaagat tgacgccgca
     1321 aacgaacagc tcaaaaagat tgcgggcgac aaggctcagg agcaaaaata cataaatgag
     1381 ctgaacgccc agatagaggc tctgcaaaag cagattaacg cctacaacag cagcatcgat
     1441 attatcgact ctcagataaa gagcctcgat aatcaaatca tagaaaccaa gagcaaaatt
     1501 agcaaaaagg aagccgaaaa gcaaaagctt gaggaagagg tagcggcgct tgagggcgaa
     1561 atcgacgata catatttaaa gctcaaagaa cgcctcagag caatttacat gaacggcacc
     1621 acatccgatt gggaaatcct gctttccggc aaggatttgt ccatgtattt cctgcgcacc
     1681 gagctgacgg agggcatagc aaagcacgac aatcagctta tagctgggct taaagagcgt
     1741 atctccgccc tccagagcaa aaaagagcag ataaagaagg aaatcgatga aataaacgag
     1801 gagcagcaaa agctcaaaca gcatcaggat accctcgatg agaaaaaaga cgcgctgaat
     1861 acccagaaga aaaaagtctc aagctccaaa agcaccataa acaccaagct tgaggaatca
     1921 accaaacttc tgaggacctt aaataaggaa agcgaaacat ataagaaact cgtaaagaaa
     1981 tacgaacagg acaaggccga attcgaggct gaaatcaacc gcatcctcaa taatgagtcc
     2041 tcaagcggtt ctggctcaat agttggcggc ggcaaaatga tttggcccgt tccttacagc
     2101 aactgctaca taacttctcc gtacggctac agaaacgacc ccttcaccgg caagtggaca
     2161 ggccatggcg gagttgacat aagcataaga ggcggagctt atggcaagaa tattgttgcc
     2221 gtgcttgacg gaactgttgt aatagctaca taccattaca gctacggtaa ttatcttgcg
     2281 atagaccacg gcaacggttt agtcaccata tacgcccact gcagcaagct gcttgtaggc
     2341 aagggcgata gggtaaaaca gggtcaggtt attgcgctta ttggctcaac gggcagctct
     2401 acaggacccc acctgcactt tgaggtcagg gtcaacaatg tccgccagga cccgatgaaa
     2461 ggctatatct cactgcccta atgccctgag aaaggaaata taaaatgagc aaaaaaatat
     2521 cattagggct tgccctggca attacctttg tctctattgc cgcagcaatt gccattacca
     2581 tgacaacctc aatgaagata tacaataacc ttataaagga ccttccggga cgctcacaga
     2641 tgtacgcgaa actcgcggaa atcgacgatt tggtcaggtc cgagttctac ggcaaaatag
     2701 acgaagaagt gcttgaggac cttttggctc aaggctatat tgccggcacg ggcgacccct
     2761 tcagccgcta tatgaccgcc gacgaatata tcagctacta caacgaaatc gccggcaaga
     2821 tgacgggtat aggtataaga ccctcccttg acagggatac gggctatata ctggttcagg
     2881 aggtttacgg cgattctccc gccgctgcag caggcatttc aaagggagat atgataaccg
     2941 ccattgacgg cgtaaaggtg gaagcctcca actaccgtga aatggtcagc aagctggagg
     3001 gcgacaagct cacaagcgtc agcgtaacct tcattcagtc ggaaacgggg caaaagctta
     3061 ccgccaacat ttccaaaggc tttacttctc gctccgtatc ctacgaaaag ctgggcagca
     3121 ttggttatat cagaataacc gacttttata aaaacaccgc ctcccagttt aaggacgcgg
     3181 tagagcgact caagagcgag tctgtaacag gtcttattat agatgtcagg aacaacagca
     3241 aaggcgaaat aagatacgcc gctgaggtta tagatgttct tgttcccctt gcgaccgagg
     3301 gcactggcgc gatagccacc gcaatcaacg ccgcgggcga ggttattgag gtttactcag
     3361 ccgactccga aaatgtaaac ctccccatgg tggttcttgt aaacaaatac tccgagggcg
     3421 gtgcggaact gcttgcctgc gacctcagag actacggcaa ggctcagatt gtgggtgaaa
     3481 caaccggcgg caagggcact ctccagcagg ttttccgcct tgacgacggc agcgcggttc
     3541 ttcttaccgt agcggaaata aagccctata taagcgagag ctttaacggc gtcggcgtca
     3601 cgcccgattt ccccgttgag ctcacactct caaaccccga agatttgtgg ctccttgacc
     3661 gagaaaagga cccccagttc caaaaggcgt atgagattct gacagaagaa taaactcaaa
     3721 aaaattaccc ttgcatttgt aatttagatg tgataatatt gcaatgttat gaagttttgt
     3781 ctgaaagagg tgacacagat gaaaaagaat atccatccta agtatcagcc cgtcactgta
     3841 aagtgcgctt gcggcgaaac attcgagacc tcttcaacca aagaagatct ccgtgttgat
     3901 atttgctcca aatgccatcc cttcttcacc ggtaagcaga agttagtaga taccggcggc
     3961 cgtgttgaca ggttcaacaa gcgctttaat atcaacgctg actaataaat agaaaaaccg
     4021 ataaaattaa gcacaaacgg cactaagttc tttggtgccg cttttggcat ttagaggtga
     4081 aaatgtctta tataacg
//
LOCUS       DAONVQ010000140         4058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_15599_length_4058_cov_4.846615, whole genome shotgun sequence.
ACCESSION   DAONVQ010000140 DAONVQ010000000
VERSION     DAONVQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15599_length_4058_cov_4.846615"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PLD98_08065"
     CDS             complement(<1..148)
                     /locus_tag="PLD98_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HQA06235.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTMRIREGDPLLITLTQFDRF
                     CAAMI"
     gene            468..626
                     /locus_tag="PLD98_08070"
     CDS             468..626
                     /locus_tag="PLD98_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HQA06236.1"
                     /translation="MAKYVCSACGYVYDPEVGDPDGGIAPGTPFEDLPDDWVCPICGV
                     SKDMFVAE"
     gene            691..1278
                     /locus_tag="PLD98_08075"
     CDS             691..1278
                     /locus_tag="PLD98_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQA06237.1"
                     /translation="MIIDSIKKRRSFYSIGKEMPVGMDKVEEIIKEAILYTPSPFNSQ
                     SARVVLLKGENHTAFWEMVRSAIKKIVSEAAFKSSSEKIDSFSAGYATILFFEDRSVV
                     KSLQNSFPLYKDNFPVWSEQSSGMLQFVIWTALCEQGLGASLQHYNELVEKDVKEKWN
                     LSPDWKLIAQMPFGGRTAEAGDKDFKPIEERFFVF"
     gene            1438..3528
                     /locus_tag="PLD98_08080"
     CDS             1438..3528
                     /locus_tag="PLD98_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002850975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HQA06238.1"
                     /translation="MKDIPAIYGSKVFNDSVMRQRLPKDTYKALRRTIKTGVPLDLEL
                     ANIVASAMKDWAVENGATHFTHWFQPMTGVTAEKHESFISLIGDGKIIMEFSGKELVI
                     GEPDASSFPSGGLRSTFEARGYTAWDPTANPFIKDKTLFIPCFFFSYSGEALDKKTPL
                     IRSIDAINNAALRVLRLFGTKASRVITTVGAEQEYFLVDAKTLEKRPDLKYCGRTLIG
                     AKPPKGQEMDDHYFGSLSGRVAAFMQELDEELWKLGIPAKTKHNEAAPAQFELACTYS
                     DANSANDSNQIIMMMMKDVAKRHGLVCLLHEKPFAGVNGSGKHNNWSLATDTGINLFE
                     PGETPGENAQFLLFLSAVIKAVDEHQDLLRLSAASAGNDLRLGANEAPPAIISMFIGE
                     ELQAILDSIENHSKYTSSGREEIRICSSVLPALPKDSTDRNRTSPFAFTGNKFEFRML
                     GSAFSISGPNIILNTIVADVLTEFAETLEKAEDFEAALNELIADTIRKHKRIIFNGNN
                     YSEEWTKEAERRGLLNLKSTVDALPYFVHPKNIELFKKHGVFSESEIFSRYEILLENY
                     IKTVNIEALTLIEMVNKGVIPSMISYETHVAKAASLMKSIDEGLSVKAEVKLLNKITA
                     LGDDIYESLGELESHMSKVGEFEDRLELARFYHENVLTAMKKVREAVDKLEVIMAKDF
                     WAYPDYGQILFGIE"
     gene            complement(3569..>4058)
                     /locus_tag="PLD98_08085"
     CDS             complement(3569..>4058)
                     /locus_tag="PLD98_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06239.1"
                     /translation="DEGAEEIKEAWTDRNCRLVVKFLKEVTHEGLTSGIMIAQGGKRL
                     SGTDIPLIKKEVPETFFRVGEGNFTDEKLNTEEGKKGLIKSLLTHKELIGDISKTYSE
                     TTVFPARALSPENLVEKMRIEIKCGLKNIYLMSGVWLIDEPYWEAIKKAMPELIALSE
                     QY"
BASE COUNT         1104 a          975 c          946 g         1033 t
ORIGIN      
        1 caatcatggc cgcacaaaag cgatcgaact gtgtcaatgt tatcagtagc gggtcaccct
       61 cgcgaattct catggttctt cttatttctt taggtataac aactctgccc aaatcatcta
      121 ttcttcgcac aatacctgtg gctttcatat tctatcctcc attttaaagt ttgccgctcc
      181 ccggagcggt tttatttgtc ttaatttcaa aaatattgtt tacagcgaaa agggaattat
      241 gccaaataat atttagtttt atttgacaaa tttagcctga attcctgccc gttcgaccaa
      301 tatttagaca tttttcagca ttgttttcac cttgtttcat ccgattctac actttgtaat
      361 tttattatac acgcaataat taaacagtta aatgatttag tctattgact aaatgtaaaa
      421 aaaaggatag gatatagaca agctatgata taaaaaggag aatcgctatg gcaaagtatg
      481 tatgttctgc atgcggttat gtttatgacc ccgaagtcgg cgatccggac ggcggaatag
      541 cacccggcac accctttgag gatcttccgg atgattgggt atgccccatt tgcggtgtaa
      601 gcaaggatat gtttgtagct gaataatttc tgggcaaaat aaagcggcag ttcaactgcc
      661 gctttgtgtt ctttaaaagg ggaggataac atgattatag attcaatcaa aaaaagacga
      721 agcttctact caataggcaa ggaaatgcct gttggaatgg acaaagtaga agaaataata
      781 aaagaggcaa tcctctatac tccaagcccc tttaactccc aaagcgcaag ggtagtgctc
      841 ttgaagggcg agaatcacac ggcgttttgg gagatggtca ggagcgcgat taagaaaata
      901 gtttccgaag cagcatttaa gtcaagcagc gagaaaatag actccttttc agctggctat
      961 gccacgatac ttttctttga ggaccggtcg gtggtcaaat cccttcaaaa ctccttcccg
     1021 ctttacaagg acaatttccc cgtatggtcc gagcagtcat cgggtatgct ccagttcgta
     1081 atctggacgg ccctgtgcga gcagggtctc ggagcctctt tgcagcacta caacgagctt
     1141 gttgaaaagg atgtaaagga aaaatggaac ctctcgcccg attggaagct tatagcccaa
     1201 atgcctttcg gcggaagaac ggcagaggcg ggcgacaagg atttcaaacc cattgaagaa
     1261 aggttctttg tattttaatt tcaaagcata atacaggctc tatttgtgct tgacaatatt
     1321 caaacaaagc attatacttt tatcaaatca gcaggacgct gggagctttt cctcgtgtcc
     1381 ttattttttt agaggctaag aaaaagcaag gattgttaga tttaaggaga ggtacttatg
     1441 aaggatatac ccgcaattta cggcagcaag gtttttaacg attccgtaat gcggcaaagg
     1501 cttcccaagg acacctataa ggctcttaga cgcacaataa agacgggcgt gccccttgac
     1561 cttgagcttg cgaatatcgt ggcaagcgcg atgaaggact gggcagtgga gaacggagcc
     1621 acccacttta cccactggtt ccagcccatg acaggcgtca ccgcggaaaa gcacgagagc
     1681 tttataagtc ttatcggcga cggcaagatt attatggagt tctcgggcaa ggagcttgta
     1741 ataggtgagc ctgatgcctc cagctttcct tcaggcggcc tcagaagcac atttgaggca
     1801 agaggctata cagcttggga cccgactgca aatcccttca taaaggacaa aacactgttt
     1861 atcccctgct ttttcttctc atacagcggc gaggctttgg acaagaaaac ccctcttatc
     1921 cgctccatag acgctataaa caacgccgcg ctcagagttc tgcggctgtt cggaacaaag
     1981 gcaagcagag ttataaccac tgtcggcgcg gagcaggagt atttccttgt ggacgccaaa
     2041 acccttgaaa agcgccccga tttaaaatac tgcggacgca cccttatcgg cgcaaagccc
     2101 cccaagggtc aggagatgga cgaccattat ttcggctccc tcagcggtcg tgtcgcggcc
     2161 tttatgcagg agcttgacga ggagctttgg aagctgggca ttcccgcaaa aacaaagcac
     2221 aacgaagcag ctcccgctca gtttgagctt gcctgcacct attcagacgc aaactccgcc
     2281 aacgacagca accagataat aatgatgatg atgaaggatg tggcaaagcg tcacggtctt
     2341 gtctgccttt tgcacgagaa gccctttgcc ggcgtaaacg gcagcggcaa gcacaataac
     2401 tggtctcttg ccaccgatac gggcatcaat ctctttgagc cgggcgaaac ccccggagag
     2461 aacgcccagt tcctgctttt cctgtccgct gtaattaagg cggtggacga gcatcaggac
     2521 cttttaaggc tcagtgccgc cagcgcgggc aacgacctga gactcggcgc caacgaagct
     2581 ccccccgcca taatttcaat gttcatcggc gaggagctgc aggccatcct cgattcaata
     2641 gaaaaccaca gcaagtatac ctcaagcggc agggaggaaa tcagaatctg ctcatctgta
     2701 ctgcccgctt tgcctaagga ttcaaccgac agaaacagga catccccctt tgccttcacg
     2761 ggcaataagt ttgagttcag aatgctgggc tccgctttct cgatttcagg tcccaacatc
     2821 atactcaaca caatagtcgc cgatgtcctg accgagtttg ccgaaaccct cgaaaaggca
     2881 gaagattttg aggcagccct caacgagctt atagccgaca ccataagaaa gcacaagagg
     2941 attatcttca acggcaacaa ctattcagag gaatggacca aggaggcgga aagacgcgga
     3001 cttttgaatc ttaaatcaac agtcgacgcc ctgccgtact ttgttcaccc gaagaatatt
     3061 gagctcttta agaagcacgg cgttttcagc gaaagcgaga ttttctcaag gtatgaaatc
     3121 ctgcttgaga attacattaa gaccgtaaat attgaggcgc tgacccttat agaaatggtc
     3181 aacaagggcg taatcccctc gatgatttcc tacgaaaccc atgtggcaaa ggcagcgtcg
     3241 ctgatgaagt ccattgatga gggtctctcc gtaaaggcgg aagttaagct ccttaacaaa
     3301 ataaccgccc tgggcgacga catctatgag agcctgggcg agcttgaaag ccatatgtcc
     3361 aaggtagggg agtttgagga ccggcttgag ctggcaaggt tctatcacga aaatgtcctg
     3421 accgccatga agaaggtaag agaggctgtg gacaaattag aggtaattat ggccaaggat
     3481 ttctgggctt atcccgatta cggacagatt cttttcggta tagaataaat aaaagccgca
     3541 gcgagtgctg cggctctttt ttttatgtct aatactgctc cgaaagagcg ataagctccg
     3601 gcatcgcctt ctttatagcc tcccagtatg gctcgtcaat cagccagacg ccgctcatca
     3661 gatatatatt tttaagcccg catttaatct caatcctcat cttttccaca aggttttcgg
     3721 gagagagggc tctcgcgggg aatacggtgg tttcgctgta ggttttgctt atatccccga
     3781 taagctcctt gtgggttaaa aggctcttta taagcccttt tttgccctcc tcggtattta
     3841 acttctcgtc agtgaaattg ccttctccga ccctgaaaaa ggtctcaggc acctcttttt
     3901 ttataagggg aatatccgtg cccgaaagcc tttttccgcc ctgggctatc attatgccgc
     3961 tggtaagccc ttcgtgggtg acttccttta aaaattttac aaccaggcgg cagttcctgt
     4021 ccgtccaagc ctcttttatt tcctcggcgc cctcatcg
//
LOCUS       DAONVQ010000141         3910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_16288_length_3910_cov_4.511284, whole genome shotgun sequence.
ACCESSION   DAONVQ010000141 DAONVQ010000000
VERSION     DAONVQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16288_length_3910_cov_4.511284"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="PLD98_08090"
     CDS             complement(<1..1348)
                     /locus_tag="PLD98_08090"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HQA06240.1"
                     /translation="MRDYKSIYEIWLKKAVADPELKKELEDIKDNDSEILDRFWRDLE
                     FGTGGLRGVIGAGTNRMNIYTVGQATQGLADYLNEHFRNPSVAIGHDSRINSDLFARR
                     AASVLAGNGIKVYFYKELKPTPMVSFAIREFKCSSGIVITASHNPSKYNGYKCYDKGG
                     YQMTDHDAGLVSEKMLKVDIFDGIKEMDFDEALEKGLIEYIPDSVSEKFYDAVLSQLI
                     EPENLKNSDIKILYTPLNGTGNIPVRTVLARAGVKNLEIVKTQELPDGKFPTCPYPNP
                     EIAQAFEEAFKLTDNYKADLIIATDPDCDRVGLAVLQDGEYVLLTGNDVCCLLTHYVL
                     TGKKRQGRLPEKPVVIKTIVTSELVRKIGESFNAEVYDLLTGFKYIGELITRLEDRGE
                     ADRFAIGMEESYGYLCGTHVREKDAVVASLLAADMAAYCKKQGITLYDYLQNLYKEY"
     gene            complement(1475..1828)
                     /locus_tag="PLD98_08095"
     CDS             complement(1475..1828)
                     /locus_tag="PLD98_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06241.1"
                     /translation="MKRNELKELIRGYIICTSLALGLFILAAGATEAANRTKMRGQGI
                     NQPIVSIDSLKSQVGEYMAAAANRKGEKKPPVTEEFRYEETEFVTVPVTFETTETTET
                     EPNTDETETTAEESS"
     gene            complement(1841..>2399)
                     /locus_tag="PLD98_08100"
     CDS             complement(1841..>2399)
                     /locus_tag="PLD98_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HQA06242.1"
                     /translation="RSKDPALNMIRVGEEIAKGLREAGFNVIHDKTIHDESYTGSYSR
                     SRQTIQKYLKQYPTIKVTLDIHRDAIHYSKTSRAKPTTVIDGKKAAQIMIITGVEEGS
                     IKNFPNWQNNLKFALKLQERAQSMFEGLMKPVMFSPRKYNMDLGEYSILLEMGTDVNT
                     LEEAAYSGRLIGVVLADLLNDYTKN"
     assembly_gap    2400..2409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2410..3059)
                     /locus_tag="PLD98_08105"
     CDS             complement(<2410..3059)
                     /locus_tag="PLD98_08105"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HQA06243.1"
                     /translation="MNRENSGFGKELKKSLVCLLSFLIVASAAYFGPQASQFLAPYAL
                     VAAGAILPEGGKKELQRIAIDNLAKVKEINGPDFLSDKKGREPVDDSAETEPSDAETT
                     EPSTAPPQPAAGDLKTTPADIAEKMKKALESEDPKKHDGKVVEREFNSKDANVIYKKV
                     YVKNSTTKDLSIKAELEKELGLEIKNRQDPAILIYHTHTTESYLLYDTGTFNSAFTA"
     gene            complement(3140..>3910)
                     /gene="gpr"
                     /locus_tag="PLD98_08110"
     CDS             complement(3140..>3910)
                     /gene="gpr"
                     /locus_tag="PLD98_08110"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR01441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HQA06244.1"
                     /translation="IQVQTEEGAQAIGKPIGKYITVEVPNFANETDLLDGRLNAIADE
                     LKSMLPTEGCVLVVGLGNNDITPDALGPKCVSQIFATRHIGSDLAMSIGLPGLRAVAA
                     LTPGVLGQTGMETSEIVAAVVERIRPTAVIAIDALAARRLSRLGNTVQMSDTGVSPGS
                     GVGNARTELSEKTLGVKVISMGVPTVVDAVTLVTDVLGEQNAARMGDIRIDQEFGRMM
                     VTPREIDLMTERAAKLIALSINTALQPSLSLDDILSLI"
BASE COUNT          771 a          980 c         1021 g         1128 t
ORIGIN      
        1 cgtactcttt gtagaggttt tggagatagt catacagtgt aattccctgc tttttgcagt
       61 aggctgccat gtcggcggca aggagagacg cgacaaccgc gtccttctct cttacatggg
      121 tgccgcaaag gtagccgtag ctttcctcca tgccgatggc gaacctgtcg gcttctcccc
      181 tgtcttcaag ccgggtgata agctcgccta tatatttaaa gcctgtgagc aggtcgtaaa
      241 cttcggcatt gaaactctcg ccgatttttc tgacaagctc cgaggtgaca atggtcttaa
      301 tgacaacggg tttttcgggg agtctgccct gccttttctt gcccgtcagc acatagtggg
      361 taagaaggca gcagacatcg ttgcccgtaa gcagcacata ctcgccgtct tggagaacag
      421 ccagacccac tctgtcgcag tcggggtcgg tggctatgat gaggtctgcc ttgtaattat
      481 cggtcagctt aaaggcttcc tcaaaagcct gagcgatttc ggggttcgga taggggcagg
      541 tggggaattt gccgtcgggc agctcctgag tttttacgat ttcaagattc ttgacgcccg
      601 ctcttgcgag aacggttctt acggggatgt tgcccgtgcc gtttaagggc gtgtagagaa
      661 tcttgatatc gctgttttta aggttttcgg gctctatgag ctggcttaaa acggcgtcgt
      721 agaatttttc ggatacggag tcggggatat actcgataag ccccttttca agagcctcgt
      781 cgaaatccat ctccttaatg ccgtcaaaga tgtccacttt cagcatcttt tcggagacca
      841 gtccagcgtc gtggtcggtc atctgatagc cgcccttgtc gtagcacttg tagccgttgt
      901 actttgaggg gttgtggctg gctgtgatga cgattccgga gctgcactta aactccctta
      961 tcgcgaatga aaccatgggc gtgggcttca gctctttata gaaatagacc tttattccgt
     1021 tgccggcgag gacggatgcc gcgcgccttg cgaaaaggtc ggagttgatt ctggagtcgt
     1081 gaccgatggc gacggagggg ttacggaagt gctcgttaag atagtcggca agaccttgtg
     1141 ttgcctgacc gacagtgtat atattcatcc tgttggtgcc ggcgccgata actcctctga
     1201 gtccgcccgt gccgaactcc aggtcgcgcc aaaagcggtc aaggatttcc gagtcgttat
     1261 cctttatatc ctcgagctct tttttaagct cggggtccgc aacggctttc ttcagccata
     1321 tttcatatat gctcttataa tctctcattg ggaacctcca aaaaaggaaa atacagtttt
     1381 tggaaacttg ctttacaaat tgtaccacac tttttgcccg cttacaatta tttatgccct
     1441 tttagccgca ataaataagg gcggtttttc cgccctaact gctttcctct gcggtggttt
     1501 cggtttcgtc cgtattaggc tcggtttcgg tggtttcggt tgtttcaaaa gtcacaggca
     1561 ctgtgacaaa ttcggtttct tcataccgaa attcctcggt taccgggggc tttttctctc
     1621 ccttgcggtt tgccgccgct gccatgtact cgcccacttg gcttttaaga gaatcaatgc
     1681 tgacaatggg ctggtttatg ccctgacctc gcatttttgt gcggtttgcc gcctcggtgg
     1741 ctcccgccgc aaggataaaa agccccaaag caagggaggt gcagatgatg taccccctta
     1801 taagctcttt aagctcgttt cttttcatgc ttcctccgtt ttagtttttc gtatagtcat
     1861 tcagaaggtc ggcaaggacg actcctatga gtctgcccga ataagccgcc tcttcaagag
     1921 tattgacatc cgtgcccatt tctaacagga ttgagtactc tcccaaatcc atattgtatt
     1981 ttctcgggga gaacataacg ggcttcataa gtccctcgaa catactctgc gctctttctt
     2041 ggagctttag ggcaaacttc aggttgttct gccagttggg gaagtttttg atgctgcctt
     2101 cctctactcc tgtgattatc ataatctgag cggctttttt gccgtctata accgttgtgg
     2161 gcttagccct tgaggttttg ctgtaatgta tggcgtccct gtgaatgtcc aaggttacct
     2221 tgatggtggg gtactgttta agatacttct gaatggtctg ccttgacctt gagtatgagc
     2281 ctgtgtatga ctcgtcgtgg atggtcttgt cgtgtattac attaaagccg gcttctctga
     2341 gccccttggc gatttcttcg cccactctta tcatgttaag ggcggggtcc ttgcttctgn
     2401 nnnnnnnnng ctgtaaaggc gctgttgaag gtgcccgtat catagagaag atagctctcg
     2461 gtcgtgtggg tgtggtaaat aagtatggcg gggtcctgcc tgttcttgat ttcgagcccc
     2521 agttcctttt ccagctccgc ttttatgctg aggtctttcg tggtagagtt tttgacataa
     2581 acttttttat aaattacatt cgcgtctttt gagttaaact ccctttcaac caccttgccg
     2641 tcgtgctttt tggggtcctc ggactcgagg gcttttttca tcttctccgc aatatcggca
     2701 ggggttgtct ttaaatcacc cgccgcgggc tgaggaggcg ctgtggacgg ctcggtcgtc
     2761 tccgcgtcag aaggctctgt ttcggcgctg tcgtcaaccg gctctctgcc ctttttatcg
     2821 gagagaaaat cggggccgtt aatctctttt acctttgcca aattgtctat ggcgattctc
     2881 tgaagctcct tttttccgcc ttcgggcaga atagcccccg ccgcgacaag ggcgtaggga
     2941 gcaagaaatt gggacgcctg cgggccgaaa tacgccgccg acgccactat taaaaaagac
     3001 aaaaggcaaa cgagactttt tttaagctct ttgccgaaac ccgaattctc cctgttcact
     3061 gtatagcccc ccgaaaaaat ctgagccgat acggcctcag gatatattta tgattttttc
     3121 ggggggttta ttctatcttt taaatgagag agagtatatc gtccaggctg agagagggct
     3181 ggagggcggt gttgattgac agggcaatga gctttgccgc cctttcggtc ataaggtcaa
     3241 tctctctggg agttaccatc attctgccga actcctggtc aatccggatg tcgcccatcc
     3301 ttgcggcgtt ctgttcgccc agaacatcgg tgactaaagt tacggcgtca acaaccgtgg
     3361 gcacacccat tgaaatgacc ttgacgccga gggtcttttc gctcagctcg gttctcgcgt
     3421 tgccgacgcc cgaaccgggg ctgacgcccg tatcagacat ctgaaccgta ttgccgagtc
     3481 ttgaaagtct gcgggcggcg agggcgtcaa tggcgattac cgctgtgggt ctgattctct
     3541 ccacaaccgc ggctacgatt tcacttgttt ccatgcccgt ctgaccgaga acgccgggag
     3601 tgagggccgc tacggctctg agaccgggca gaccgatgga catggcaagg tcgctgccta
     3661 tatgccttgt ggcgaaaatc tggctgacgc acttgggtcc caaagcgtcg ggagtgatgt
     3721 cgttgtttcc gagaccgacg accagaacgc agccttcagt gggcagcatg gatttcagct
     3781 cgtcggcaat ggcgtttagc ctgccgtcaa gcaggtctgt ttcgtttgcg aaattgggca
     3841 cctcgactgt tatatacttg cctatgggct tgccgatagc ctgtgcccct tcttcggtct
     3901 gaacctgaat
//
LOCUS       DAONVQ010000142         3868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_16470_length_3868_cov_4.818516, whole genome shotgun sequence.
ACCESSION   DAONVQ010000142 DAONVQ010000000
VERSION     DAONVQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16470_length_3868_cov_4.818516"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLD98_08115"
     CDS             complement(<1..320)
                     /locus_tag="PLD98_08115"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HQA06245.1"
                     /translation="MYSLNLVLVEPEIPQNTGNVARTCAVTGASLHLVEPMGFKIDDK
                     KLKRAGLDYWHMLDIHYHASLEDFMKTIEGKPFYCFSTKAPLVYTEVQYPDEVYLLFG
                     KETAG"
     gene            complement(467..1702)
                     /locus_tag="PLD98_08120"
     CDS             complement(467..1702)
                     /locus_tag="PLD98_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQA06246.1"
                     /translation="MIKTLRRKFIFIMMSMVSLLLITIFMTVIVATSSSIQEENINKL
                     RQILQTGVVQDYYGNAVDARSRMNVIVVELYADGSYYVATNQFLLLDDNLLSDIVKQA
                     AKSQSDSGELRQYDLRFLKHQTSNTLRIAFADTSMERSIIRSLALVCILICTMSLLAF
                     FILSVFLAGWAVRPVENAWMRQKRFVADASHELKTPLTVILSNAEMLHNSGLQPDSKA
                     AERVDNILAEGIRMKKLIENMLSLAKSDYAENALILSKVNLSDIILDSVLLYESAAYD
                     DKKQLFYNVEEGIIVNGDADKLRQVIDILLDNALKYSKEGASITVSLTSQFSIHYRKE
                     AKLVVRNEGEPIPKNELENIFIRFYRVDKARENHGGFGLGLSIAQEIIQSHKGRIWAD
                     SDSTGNVFSVTLPLASSYF"
     gene            complement(1695..2372)
                     /locus_tag="PLD98_08125"
     CDS             complement(1695..2372)
                     /locus_tag="PLD98_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQA06247.1"
                     /translation="MRILIVEDEVRLARTLKDLLEQHNHIADMSFDGESGLDNALSGI
                     YDAVILDVMLPKLDGFGVIKGMREAGLQTPVLMLTARSELENKVKGLDLGADYYLTKP
                     FEAAEFLACLRAILRRRGEIQPDELKFADLTLNINSAELSCEGRRVRLRAKELELLRI
                     LMENGNKFVPKETLQLKVWGYDSEAEGNVVEVYMSFLRRKLSHIKSKVKITSSRMIGY
                     RLEADND"
     gene            2507..3328
                     /locus_tag="PLD98_08130"
     CDS             2507..3328
                     /locus_tag="PLD98_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06248.1"
                     /translation="MIVVYEKQLISKDGNPETNEDGIFINDSYIAVVDGVTAKGKLKW
                     NGKKSGRHARDIILEALKKAPADFRAREMILYLNRSLFDEYGENTQFFKENKEERLQA
                     NIILYSAFKKEVWCFGDCRCIVNGEIHLHEKAIDRFTSELRSKFLEEEIKRGKTVEEL
                     MRHDTGREHIMPYLLKQTDYANAKGEFGYDVLDGFEIDADRTVIYKPEANSEIVLASD
                     GYPVLRGTLEESERELQYILSNDKLLFRLHKETKGLISGNLSFDDRAYIRFTDTN"
     gene            complement(3358..>3868)
                     /locus_tag="PLD98_08135"
     CDS             complement(3358..>3868)
                     /locus_tag="PLD98_08135"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HQA06249.1"
                     /translation="IILNSSLVPHYPLPFGGSVTIFGQLPIVIIAYRYGTKWAFHSAV
                     LFGFTQLLFGWGNAAAIAKTFISYLIFILFDYLLAFGVLGLAGMFKNKIKNAPLSLAL
                     GSIVASALRFLCHFISGVTIWSAYADERGVLVYSLAYNASYMVPEMILTAIGAFAVAG
                     VLKLQKKNR"
BASE COUNT         1022 a          888 c          905 g         1053 t
ORIGIN      
        1 cccgcggttt cttttccgaa aagcagatac acctcatcgg gatactgaac ttcggtatac
       61 acaaggggcg ccttggtgga aaaacagtaa aagggcttgc cttctattgt tttcataaag
      121 tcctcaagtg aggcgtgata gtggatgtca agcatgtgcc agtaatcaag acctgccctt
      181 ttcagcttct tgtcgtcaat cttaaagccc ataggctcca caagatgaag ggacgcgccc
      241 gttaccgcgc aggttcttgc cacattgccc gtgttctgcg gaatttcggg ttctaccagt
      301 acaaggttta gggaatacat aagctttccg ttttcctgcc ttccaaaaag agaatatgaa
      361 cggaaatgat actccttcgg ctgctcttaa attgcctatt aatcattctt cacattctct
      421 ttatatcata atttatcaaa atgatgataa aatgacgcaa aaaaaattaa aaatagctgc
      481 ttgccagagg gagggtaacg ctgaaaacat ttccggtgct gtcgctgtcc gcccagattc
      541 tgcccttgtg gctctggata atctcctggg cgatggaaag accgagaccg aaaccgccgt
      601 ggttttctct tgccttgtcc actctgtaga agcggatgaa aatgttttcc agctcattct
      661 taggtatcgg ctcgccttcg tttcttacta ccagctttgc ctcttttctg taatgaatgc
      721 tgaactggga ggtaaggctc acagtaatgc ttgcgccctc ctttgagtat ttgagcgcgt
      781 tgtcaaggag gatgtcaata acctgcctta atttgtcggc gtcgccgttt acgataatgc
      841 cttcctccac attgtagaaa agctgctttt tatcgtcgta ggcggcggat tcatacaaaa
      901 gcacgctgtc aaggataatg tcgctgaggt ttacttttga aagaataagg gcgttctcgg
      961 cgtaatcgga tttcgcgagg ctcagcatat tttcaatgag ctttttcatt ctgatgcctt
     1021 ccgcaaggat gttgtccacc ctttcagccg ccttgctgtc gggctgcaga ccgctgttat
     1081 gaagcatctc cgcgtttgac agtattaccg tcaagggagt tttaagctca tgggaggcgt
     1141 ccgccacgaa tcttttttgg cgcatccacg cgttttcaac gggtcttacc gcccagcccg
     1201 caaggaaaac gctgagaatg aagaacgcga gaagcgacat ggtgcaaata agtatacata
     1261 caagagcgag gctccttatt atgcttctct ccatggatgt gtcggcaaag gctattctga
     1321 gtgtattgct tgtctggtgc tttaagaatc tgaggtcgta ctgtcttagt tccccgctgt
     1381 cgctttggct tttagccgcc tgctttacaa tatcggagag caagttatca tccaaaagca
     1441 aaaattggtt tgtggcaaca taataggagc cgtcggcata tagctccacc acaattacat
     1501 tcattcttga ccttgcgtca acggcattgc cgtaatagtc ctgcacaacg cctgtttgca
     1561 gaatttgcct tagtttattt atgttctctt cctgtatgct gctgcttgtc gccacgataa
     1621 cggtcataaa aatcgtgatg agcaataggc ttaccatgct catcataata aagatgaatt
     1681 ttctgcgcag cgttttaatc attgtctgcc tccaagcgat agccgatcat gcgggacgat
     1741 gttatcttta cctttgattt tatatggctc agctttcttc ttaaaaagga catatagacc
     1801 tctaccacat tgccctccgc ttcggagtcg tagccccata cctttagctg gagggtttct
     1861 ttagggacaa acttattgcc gttttccata agtatgcgaa gaagttccag ctcctttgcc
     1921 ctgagcctta ctctcctgcc ctcgcagctg agctcggcgg agttgatgtt aagagtcaaa
     1981 tccgcgaact ttagctcgtc cggctggatt tctcccctgc gcctgaggat tgctctgagg
     2041 caggcaagga attccgccgc ctcaaagggc tttgtaaggt agtagtcggc gcccaaatca
     2101 agacccttga ccttgttttc aagctccgac cttgcggtga gcatcaaaac gggggtctgg
     2161 agtcccgctt ctctcatccc ttttatgacg ccgaaaccgt caagcttcgg gagcattaca
     2221 tcaaggataa cagcatcata aattccgctg agggcgttgt caagcccgct ttcgccgtca
     2281 aaagacatat ccgctatatg gttatgctgt tccaaaaggt ctttcagcgt tctcgcaagc
     2341 cttacttcat cctctactat cagaattctc atatattaca catcctctga tgggagtatt
     2401 cgtacctata ttatacaccc aataattaaa ctcagcttaa acttgcgctt taatgtataa
     2461 taatttggtg atataatttt tgtaaactcg gacaggacag atgcgaatga ttgttgtata
     2521 tgaaaaacag cttatttcaa aggacggaaa tcccgaaaca aacgaggacg gaattttcat
     2581 aaatgacagc tatattgcgg ttgtggacgg cgttacggct aagggaaagc tgaaatggaa
     2641 cggcaagaaa agcggcaggc acgcaaggga tattatactc gaggcgttaa aaaaagcccc
     2701 tgcggacttt agagcaagag aaatgattct ttatcttaac cgctcccttt ttgatgaata
     2761 cggagagaat actcagtttt tcaaagagaa taaagaggag cggctgcagg cgaatataat
     2821 tctttattcg gctttcaaaa aagaggtttg gtgctttggc gactgccgct gcattgtgaa
     2881 cggagaaatt caccttcacg aaaaggcaat tgacaggttt acttctgagc taagaagcaa
     2941 attccttgag gaagaaatta aaaggggcaa aaccgtagag gaattaatgc ggcacgatac
     3001 gggcagggaa catattatgc cctatctttt aaagcagacc gactatgcca acgcaaaggg
     3061 cgagttcggc tatgatgtgc ttgacggctt tgagattgat gccgaccgca ccgtaattta
     3121 taaacccgag gcgaattcgg aaattgtgct tgcttccgac ggctatcccg ttctgagggg
     3181 aacacttgag gaaagcgaaa gggaacttca atacatactc tcaaacgaca agctgctgtt
     3241 caggctccat aaggaaacaa aaggtttaat cagcggcaat ctttcctttg acgacagggc
     3301 ttacatcaga tttaccgaca caaattaaaa gctgcggcgt tttgccgcag ctatgtacta
     3361 tcgattcttt ttttggagct tcagcactcc ggcgacggcg aaggctccta tggcggtgag
     3421 tatcatctcg ggcaccatgt atgaggcgtt gtacgcaagg gaataaacaa gcactcccct
     3481 ttcatccgca taggcgctcc atatggtgac accgcttata aagtggcata aaaatcttaa
     3541 agcggacgcc actattgagc ccaaggctaa ggacagcgga gcgtttttta tcttgttttt
     3601 gaacattccg gcaagtccca aaactccgaa ggcaagaaga taatcaaata gaatgaagat
     3661 taagtaagaa ataaaggtct tagctattgc ggcggcgttg ccccagccga aaagcagctg
     3721 cgtaaagccg aaaagcaccg cggaatggaa cgcccatttg gttccgtagc ggtaggctat
     3781 aatgactatg ggaagctgac cgaaaatagt cacgctgccg ccaaagggca gggggtaatg
     3841 tggcacaagg ctgctgttga gtatgatt
//
LOCUS       DAONVQ010000143         3865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_16490_length_3865_cov_4.906824, whole genome shotgun sequence.
ACCESSION   DAONVQ010000143 DAONVQ010000000
VERSION     DAONVQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16490_length_3865_cov_4.906824"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2019
                     /gene="pflB"
                     /locus_tag="PLD98_08140"
     CDS             <1..2019
                     /gene="pflB"
                     /locus_tag="PLD98_08140"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HQA06250.1"
                     /translation="TVSTIVSHKPGYINKELEEIVGLQTDEPLKRAIMPFGGIRLVRT
                     ALEAYGRQLPDDIEQIFKYRKTHNDGVFDVYTDEMRRARKSGIITGLPDAYGRGRIIG
                     DYRRVPLYGVDALIEQKRREKESFVFDTMDEMEIRQREELSEQIKALEELKEMAASYG
                     FDISRPAEDIKEAIQWLYFGYLAAVKDQNGAAMSIGRISTFLDIYAERDLKSGKYTEE
                     QIQEFVDHFIMKLRIVRFLRTPAYDQLFSGDPTWVTESLGGMCVDGRHMVTKMTYRFL
                     HTLTNLGPAPEPNLTVLWSTRLPENFKRYCAKMSIVTSSIQYENDDIMRPKFGDDYGI
                     ACCVSAMKIGKQMQFFGARANLAKALLYAINGGRDEVTGMQVGPEFAPITSEYLDYDE
                     VVKKFDTVCDWLAKLYINTLNCIHYMHDKYCYERLEMALHDVNIVRTMACGLAGLSVV
                     ADSLSAIKHAKVKVVRNEEGLATDFIVEGDYPKFGNNDDEIDQIAVNIIKAFMNKLRR
                     HKTYRDSIPTLSVLTITSNIVYGKKTGSTPDGRKHGEPFAPGANPMHGRDTKGCVASM
                     KSVAKLPYDYAEDGISYTFSIVPGALGKTDDDRVTNLVGLLDGYFGDTGHHINVNVIN
                     KEVLMDAMEHPEKYPQLTIRVSGYAVNFIKLTREQQLDVLHRTFHERF"
     gene            2053..2787
                     /gene="pflA"
                     /locus_tag="PLD98_08145"
     CDS             2053..2787
                     /gene="pflA"
                     /locus_tag="PLD98_08145"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HQA06251.1"
                     /translation="MGVLGRVHSIESFGTLDGPGIRYVLFLQGCNFRCLYCHNPDTWA
                     TCTGKTMDSDDVIRDILSYKNFIKSGGVTLSGGEPLIQADFAAAILKGCREHNIHTAL
                     DTAGSIPLSKSAPVLDLSDLVLLDIKSFDDNVARKISGYTNKRTLETLNYCEEKGIKV
                     WMRQVLLPGYTLDYETLSALADYLKGFECVELIELLPFHKMGEYKWEQLGFDYELKDT
                     QPPTPEELLKARQVFENKGIKVLMKG"
     gene            3026..>3865
                     /locus_tag="PLD98_08150"
     CDS             3026..>3865
                     /locus_tag="PLD98_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA06252.1"
                     /translation="MSLINIQNLTFSYEGSYDNIFENVSFQIDTDWKLGFIGRNGRGK
                     TTFLNLLMGKYEYSGTISASVDFEYFPYKVRDDSQNTIDVLGEIYPDFVYWQLQREMS
                     LLEVDEDVLYRPFSTLSKGEQTKVLLSILFLKENSFLLIDEPTNHLDMRGRELVSRYL
                     NRKKGFILVSHDRAFLDGCVDHILSINRANIEIQKGNFSSWLENKERQDKYELAENEK
                     LKKEIKRLKEAAREKAQWADKAEREKIGADPRKVDNTVGRRPYLAAKSKKMMKRAKAI
                     EKRS"
BASE COUNT         1086 a          989 c          960 g          830 t
ORIGIN      
        1 accgtatcca caattgtctc ccacaagccc ggctatatca acaaggagct tgaggagatt
       61 gtgggtcttc agaccgacga gcctttaaag agggcaatca tgccctttgg cggcataaga
      121 cttgtcagaa ccgcccttga ggcatacgga agacaattgc ccgacgacat cgagcagata
      181 ttcaaatacc gcaagaccca caacgacggc gtttttgatg tgtacaccga cgagatgaga
      241 agggcaagaa agagcggcat catcacgggt ctgcccgacg cctacggcag aggcagaatc
      301 ataggcgact accgcagagt gcctctctac ggcgtagacg cccttataga gcagaagaga
      361 agagagaagg aatcctttgt ctttgacaca atggacgaaa tggaaatccg ccagagagaa
      421 gagctcagcg aacagattaa agccctcgag gagcttaaag aaatggcggc aagctacggc
      481 tttgacattt ccagacccgc cgaagacata aaagaggcaa tccagtggct ctatttcggc
      541 tatctcgccg cggtcaagga ccagaacggc gccgccatga gcataggcag aatatccacc
      601 ttccttgata tatacgctga gcgtgacctc aagagcggca aatataccga ggagcagatt
      661 caggagtttg ttgaccactt tattatgaag cttcgcatcg tccgcttcct cagaaccccc
      721 gcttacgacc agcttttcag cggcgacccc acatgggtaa ccgagtccct tggcggcatg
      781 tgcgttgacg gcaggcacat ggtcacaaag atgacctatc gtttcctgca cacgctcacg
      841 aatctgggcc ccgctcccga gccgaacctt acggtccttt ggagcacaag gctgcccgag
      901 aactttaagc gctactgcgc aaagatgtcc atcgtcacat cctccattca gtacgagaat
      961 gacgatataa tgcgtcccaa attcggcgac gactacggca tagcctgctg cgtctccgcc
     1021 atgaagatag gcaagcagat gcagttcttc ggcgcgagag caaacctcgc caaggctctg
     1081 ctctacgcca tcaacggcgg acgcgacgag gtaaccggta tgcaggtcgg tcccgagttc
     1141 gctcccatca cctccgagta ccttgactat gacgaggttg tcaaaaagtt tgacaccgta
     1201 tgcgactggc tggcaaagct ctatatcaac accctcaact gcatccacta tatgcacgac
     1261 aagtactgct acgagcgtct tgaaatggcg ctgcacgatg tgaacatcgt cagaaccatg
     1321 gcatgcggac ttgcgggtct gagcgttgtt gctgacagcc tttcagccat taagcacgcc
     1381 aaggtaaagg ttgtaaggaa cgaagaaggt ctcgccacag actttatagt agagggcgac
     1441 tatcccaagt tcggcaacaa cgacgacgag atagaccaga tagcggtcaa catcataaag
     1501 gcgtttatga acaaactgcg ccgccacaag acttacaggg attcaatccc caccctttcc
     1561 gtgcttacga ttacctccaa catcgtttac ggcaaaaaga cgggctcaac ccccgacggc
     1621 agaaagcacg gcgagccctt cgcccccgga gccaacccga tgcacggcag agacaccaag
     1681 ggctgcgtcg ccagcatgaa gagcgtcgcc aagctcccct atgactacgc agaggacggc
     1741 atttcctaca ccttcagcat tgtacccggc gctctgggca agacggacga cgacagagtc
     1801 acaaaccttg tcggtctcct cgacggctat ttcggcgata cgggccacca tatcaatgta
     1861 aatgtaataa acaaggaagt ccttatggac gccatggagc atcccgaaaa atatccgcag
     1921 ctcacaatca gggtcagcgg atacgcggta aacttcataa agcttacaag agagcagcag
     1981 cttgatgtcc ttcacagaac cttccacgag aggttttaat taagcctttg agctttttga
     2041 gaggatagaa agatgggagt tttgggcaga gttcattcaa tagagtcctt cggaaccctt
     2101 gacgggccgg gcatcagata tgtcctcttc ctgcagggct gcaatttccg ctgcctgtat
     2161 tgtcacaacc ccgacacatg ggctacctgt acgggcaaga ctatggactc cgacgatgtt
     2221 attcgggata ttctcagcta caaaaacttt attaaatccg gcggagtcac cctgtcgggc
     2281 ggcgagcccc ttattcaagc cgactttgcc gccgccatct taaagggctg cagggagcat
     2341 aatatccaca cagcccttga caccgcggga agcatccctc tttcaaaaag cgcccccgtt
     2401 ctggatttgt cagaccttgt gctgcttgac ataaaaagct ttgatgacaa tgtggcaagg
     2461 aaaataagcg gctacacaaa caaaaggacc cttgaaaccc taaactactg cgaggagaag
     2521 ggcataaagg tttggatgcg tcaggttctt ctgcccggct acaccttaga ctatgaaacc
     2581 ctctcagccc tcgcggatta tctcaaaggc tttgagtgcg tggaactcat agagcttctg
     2641 ccctttcaca aaatgggcga gtacaagtgg gagcagctgg gctttgacta cgagctgaaa
     2701 gacacccagc ccccaacgcc cgaggagctg ttgaaagcca gacaggtttt tgaaaataag
     2761 ggcataaaag tattaatgaa gggataaaac ccttcacttt tcagaaaaat atggtataat
     2821 tacagaaacg acataaaggg aggtcaaagg atgttcaatc tttcttgcta aactgaaggc
     2881 atagcataaa aaggcgatat attcgctctc tttgttatgc cgtttgcaag aaagttgatt
     2941 cttttggcca aacggtttta aagctcattg tcggctgcgg gaatttactt tcttgcggcc
     3001 gatttttatt ttgggagtga gccttatgtc gctaataaac attcagaacc taaccttcag
     3061 ctatgagggc agctatgaca atatttttga gaatgtaagc tttcagatag ataccgactg
     3121 gaaactgggc tttataggca gaaacggcag aggaaaaacc acctttttaa atcttcttat
     3181 gggcaaatac gagtacagcg gcacaatatc cgcaagcgtg gactttgagt atttccctta
     3241 taaagtgcgg gacgacagcc aaaacaccat tgatgttctg ggcgaaatct accccgactt
     3301 tgtctattgg cagcttcaaa gggagatgag cctattggaa gtggacgagg atgtgctgta
     3361 tcgccccttt tccacccttt caaaaggtga gcagaccaag gtgcttcttt caattctctt
     3421 tttaaaggag aacagttttc tgctcattga cgagcccaca aaccaccttg acatgagggg
     3481 cagggaactt gtaagcaggt atctcaaccg caaaaaaggc ttcattcttg tttcccacga
     3541 cagagccttc cttgacggct gcgtggacca tattctctcc atcaacaggg cgaatatcga
     3601 gattcaaaag ggcaatttct cctcatggct cgagaataaa gagcggcagg acaaatatga
     3661 gctggccgag aatgagaagc tgaagaagga gattaaacgc cttaaagaag ccgcgagaga
     3721 aaaggcgcag tgggcggaca aagccgaaag agagaaaatc ggcgccgacc cgaggaaggt
     3781 tgataacacc gttggccgca gaccctacct tgcggcgaaa tccaagaaaa tgatgaagcg
     3841 ggcaaaggcg atagagaagc gttcc
//
LOCUS       DAONVQ010000144         3857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_16532_length_3857_cov_5.188848, whole genome shotgun sequence.
ACCESSION   DAONVQ010000144 DAONVQ010000000
VERSION     DAONVQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16532_length_3857_cov_5.188848"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLD98_08155"
     CDS             complement(<1..550)
                     /locus_tag="PLD98_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06253.1"
                     /translation="MGINLNYNVSAHRVLHDQKMKDMLPIMRDTGVSTLWLFGYFYGR
                     FESELSEIEKAKKYLESEGFEVNAISIPVGHPGNALDGDISSHTDLTIPDTWHYRVNG
                     EGKNTYFCAAIDDVMIKDNAEAAKKLRDIGITKIFYDDDLRTSSSSSEIGGCFCEKCI
                     EEFNSETGHNFDREILYGILTSD"
     gene            complement(781..>3857)
                     /locus_tag="PLD98_08160"
     CDS             complement(781..>3857)
                     /locus_tag="PLD98_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06254.1"
                     /translation="FEFGMIFDISLPWILNLLEDQFGEGLEFADILGGTFDYLFTRVA
                     MPYTSANGLTAYRTYGVLDNVDVFTCVLTMLLEVVLHGNNAEILDEMLGTDGILPAVT
                     GLFDGLINDMGEINWLWFSDDYTGPLYGDSDVQLPERSIVYLTYPNNWSKGVANYLDA
                     NLADIVDSVISTIDDDYASLAEMLADQVDFYNDDTVNSLLKVFKDLIADIDRALLDTV
                     GVVLELDLTAWDAYKYDHEWGVTDRESFIDALCEVLMPLAEAVDWLLYGDDYNFFTGS
                     EVSGEEGRDIIMIKGADGYAYGLVPLLEALGCKNVLSQTAIKEELAANPQSPVRFKAI
                     LEPLLARLDEIVANPVDEILGIIPNLMFFINANGLSVSVKNTLHAVLNIFEAVAPALD
                     TSLDELIGFSIDDLTFTGIFTILKDKTGIDLNAPIGEFMSTFWLGDLEVYTSANGNGA
                     IRMSYSEDEHRGDLITIIFCLLLQVIEHENNAEILRDMLGSDTYDAVLNVLNLQEFPM
                     QEISWYNTEYADTGVVFSALNTSELFSSSYGRLWTKDKADYIASNFGTTVDNIIKLLG
                     IEGENGQLLKSLNELINDLADGNIYTSANVNSVAEMFQDFVAQIDELGEAGEHAKALI
                     YEIFSIDLNHWNQFGPDYDWGFENGDRAAFIAAVAEVLSPLNPILEWLLCDKDISFFV
                     DANGEDAVTFLGAEGYAYGIIPLLEALHCENILTPAEYYAAVDENPNNLIIAILTPLF
                     AKIDELLADPANKILEMLPGIIYFVNSNGLDTAFKNILHPVYGILHAIEPVVQIDLYD
                     MLGIRLDEYNFESLYKLALSSIAESTGFSFDELVGNAINELALGTIVSFQSKNGQTAY
                     TMVYAPQADQGDMVTILLRLGIKFLTYGNNIETAKNLLRQTDINPEAKKYLLALLETM
                     AELSEDPQAQDTIMGSMYYVFVGVEAGSGGANDIIDQINSEWKAIINALLNSDSELLR
                     NMGKAIAELLNENFDGIFDEGGLASGGLLKFFAKIREFFENILNFFRNLMRY"
BASE COUNT          898 a          897 c         1029 g         1033 t
ORIGIN      
        1 catcggaggt cagaatgccg tacagaatct ctctgtcaaa attatgtccc gtttcgctgt
       61 tgaactcctc aatgcacttt tcgcagaagc agccgccgat ttcagaggag gatgaagatg
      121 tgcgaaggtc gtcgtcatag aaaatctttg tgatgccgat gtccctgagc ttctttgccg
      181 cctcggcgtt gtccttaatc ataacatcgt caatagcggc gcagaagtag gtgttcttgc
      241 cctcgccgtt tactctgtaa tgccatgtgt cgggtatagt aaggtcggta tggctggaaa
      301 tatccccgtc aagggcgttg cccggatgac ctacgggaat ggaaatggcg tttacctcaa
      361 aaccctcgga ttcaagatac ttctttgctt tctcaatttc cgaaagctcg gactcgaacc
      421 ttccgtaaaa ataaccgaac agccacaggg tagaaactcc ggtgtcacgc attatcggga
      481 gcatatcctt catcttttgg tcatgcagaa ccctgtgggc actcacatta taattcagat
      541 ttatgcccat taaattgcca cccttggttt tttatttagt gtatcataaa aggggagaga
      601 gtcaacgcat tttatgtaaa tagatttcat tttattatta aaaagcgcaa aggccaaaat
      661 tttcatttaa aggcccttat tagcgttctt tagtaattaa ttataaagtt taattaagaa
      721 tactctaaaa agcaaaaata cagcccctgt gccaaaagca caggggcttt gagttggttt
      781 ttagtatctc atcagatttc tgaagaagtt gagtatgttc tcgaagaact ctctgatctt
      841 tgcgaagaac ttcaggagtc cgccggatgc gagaccgcct tcgtcaaaga tgccgtcgaa
      901 gttctcgttg aggagttcgg ctattgcctt acccatattc ctcagaagct ctgaatcgga
      961 gttcagaagt gcgttgatta ttgccttcca ctcggagttg atctggtcga ttatgtcgtt
     1021 tgcgccgccg ctgccggcct caacaccaac gaatacatag tacattgaac ccatgatggt
     1081 gtcttgtgcc tgcgggtctt cgctaagctc agccatggtc tcgagcagag cgagcaggta
     1141 cttcttagcc tcaggattga tatcggtctg tctgagcagg ttcttggctg tttcgatgtt
     1201 gttgccgtag gtcaggaact tgatgcccag tctgaggagg atggttacca tgtcgccctg
     1261 gtcagcctgc ggagcataga ccattgtgta agcggtctga ccgttcttgg actggaagga
     1321 aacgattgta ccgagtgcca gctcgttgat tgcattgcct acaagctcgt cgaaggagaa
     1381 gcctgtgctt tctgcaatgc tgctgagtgc cagcttgtag agggactcga agttgtactc
     1441 gtcgagtctg atgccgagca tatcatacag gtcaatctga acaaccggct cgatcgcatg
     1501 caggattccg tatacaggat gcaggatgtt cttgaatgcg gtgtcgagac cgttggagtt
     1561 gacgaagtag atgatgccgg gcagcatctc gagaatcttg tttgcgggat cggcgagcag
     1621 ctcatcgatc ttagcaaaca gcggagtgag tattgctatg atcaggttgt tcgggttctc
     1681 atcaactgct gcgtagtact ctgccggtgt gagaatgttc tcgcagtgga gagcctcaag
     1741 cagcgggatg ataccgtatg cataaccctc agcgccgagg aatgttacgg cgtcctcacc
     1801 gttagcgtct acgaagaagg agatatcctt atcgcagagt agccactcaa gaatcggatt
     1861 cagcggtgag agaacctcag caactgctgc gatgaatgcg gctctgtcgc cgttctcgaa
     1921 gccccagtca tagtcgggac cgaactggtt ccagtggttc aggtcgatgc tgaagatctc
     1981 gtagatgagt gcctttgcgt gttcgccggc ctcacccagc tcatcgatct gagctacgaa
     2041 atcctggaac atctcagcaa cgctgttcac attggctgat gtgtagatgt tgccgtcagc
     2101 aaggtcgtta atcagttcgt tgagggattt taacagctgg ccgttctcgc cctcgatgcc
     2161 gaggagcttg atgatgttgt caactgtggt gccgaagttg cttgcgatgt agtcagcctt
     2221 atccttggtc cacagtctgc cgtagctgga ggagaagagt tctgatgtgt tcagtgcgga
     2281 gaatactacg ccggtgtctg cgtattcggt gttgtaccag ctgatctcct gcatcgggaa
     2341 ctcttgcagg ttgagtacat taagtactgc gtcgtaggtg tcgctgccga gcatatctct
     2401 gagtatctcg gcgttgttct catgctcaat tacctggagg agcaggcaga agatgattgt
     2461 aatcaggtcg cctctgtgct cgtcttcgct gtagctcatg cggattgcgc cgttgccgtt
     2521 ggcgctggtg taaacctcaa ggtcgccgag ccagaaggtt gacataaact caccgatagg
     2581 tgcgttcagg tcaatacctg tcttatcctt caggatggtg aatatgcctg tgaaggtcag
     2641 gtcatcaatt gagaagccta taagctcatc aagtgatgtg tcaagtgcgg gagctactgc
     2701 ttcgaagatg ttcagaacag cgtggagcgt gttctttacg gagactgaga gaccgtttgc
     2761 gttgatgaag aacatgaggt tcgggatgat tccgagaatc tcatcaacag gatttgcaac
     2821 gatttcgtca agtcttgcga gcagcggctc aaggattgcc ttgaatctta cgggtgactg
     2881 cgggttggct gcgagctctt ccttaatagc tgtctggctc agaacattct tgcagccgag
     2941 agcctcaagg agcggtacca gaccgtatgc gtatccgtca gcgcccttga tcattatgat
     3001 gtctctgcct tcctcgccgg aaacctctga gccggtgaag aagttgtaat catcaccgta
     3061 gaggagccag tcaacagctt ccgcaagcgg catgagaact tcacagaggg catctatgaa
     3121 ggactctctg tcggttacgc cccattcatg gtcgtacttg taagcatccc atgcggtcag
     3181 gtcaagctca agaactacgc ctaccgtgtc aagcagtgct ctgtcgatgt cggcgataag
     3241 gtccttgaat accttaagga gtgagttgac tgtgtcgtca ttgtagaagt caacctggtc
     3301 agccagcatt tctgccagtg atgcgtagtc gtcgtcgatg gtgctgatta cggaatcaac
     3361 gatgtcggcg aggtttgcgt caaggtagtt tgctacgccc tttgaccagt tgttcgggta
     3421 ggtcaggtag acgattgaac gctcaggcag ctgaacatct gaatctccgt aaagcggacc
     3481 ggtgtagtca tcggagaacc agagccagtt aatctctccc atgtcgttga taagtccatc
     3541 gaagagacct gttactgccg gcagtatgcc gtctgttccg agcatctcgt caagtatctc
     3601 tgcgttgttg ccgtggagga ctacctcaag cagcattgta agaacgcagg tgaatacatc
     3661 tacattgtca agtacaccgt aggttctgta agctgtcaga ccgtttgcgg aggtgtaggg
     3721 cattgcaact cttgtgaaca ggtagtcgaa ggtgccgccg agtatgtctg cgaactcaag
     3781 accttcgccg aactgatcct caagcaggtt aagaatccac ggtagtgaga tgtcaaagat
     3841 catgccgaac tcgaact
//
LOCUS       DAONVQ010000145         3843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_16596_length_3843_cov_4.078405, whole genome shotgun sequence.
ACCESSION   DAONVQ010000145 DAONVQ010000000
VERSION     DAONVQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16596_length_3843_cov_4.078405"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /gene="alr"
                     /locus_tag="PLD98_08165"
     CDS             <1..828
                     /gene="alr"
                     /locus_tag="PLD98_08165"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQA06255.1"
                     /translation="QLSDTAVRMGKKAKIHVVVDTGMSRIGFPPTEESVETIKWINTL
                     PGLFVEGIFSHFALADSKDKTVDKMQLKRFGFVLDGLEREGIHIPIKHISNSAAVIDS
                     DYSFDMVRCGIMLYGMYPSDEVDKTKVSLKPVMSLKSSVIQVKTLPPGEGVSYGHTFV
                     TTRETRIATVSAGYGDGYPRALSSKGRVIINGQYAPIIGRVCMDIFMVDISHIDGVKV
                     EDPVILIGRDGDKEITAEEVGALADTFNYEIVCSVSRRVPRIYLKNSVEVGEYCAFK"
     gene            complement(864..1613)
                     /locus_tag="PLD98_08170"
     CDS             complement(864..1613)
                     /locus_tag="PLD98_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQA06256.1"
                     /translation="MNLNNVIAVRAGKTVYKDGDYAIKVFDENYKKSDILNESLNQAR
                     VEETGLNIPKVQEVLKIDGKWAIVFDFIPGKTLQQLMDENPDKLNEYLNLFVDVQLDM
                     HSRRAPLLGKLKDKMNRKISESGLDATTRYELHTRLNSLPTHNKVCHGDFNPSNIIIH
                     NNGTPYILDWPHATQGNASADAARTYLLFWLSGNEKLAEEYLKLFCLKSDTAKQYVQK
                     WVPIVAASQLVKGKPEEREFLLRWVNVLDFQ"
     gene            complement(1722..>3843)
                     /gene="adhE"
                     /locus_tag="PLD98_08175"
                     /gene_synonym="adhC"
     CDS             complement(1722..>3843)
                     /gene="adhE"
                     /locus_tag="PLD98_08175"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HQA06257.1"
                     /translation="AVKAGAPEGIIRWIDVPSLELTNLVMREADIILATGGPSLVKAA
                     YSSGKPAIGVGAGNVPAVIDESADIKLAVNSIIHSKTFDNGMICASEQAVIVPEKIYM
                     AVKDEFEKRGCYFLKGDELDKVRKTIIINGALNAKIVGQPAYRIAELAGIEVPKDTKI
                     LIGEVTSVELSEEFAHEKLSPVLAMYKAKDFDEAIEKAARLIEDGGLGHTSSLYVDAF
                     NAKEKAEKFERAMKTCRVLINTPSSHGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLINIKTVAERRENMLWFRTPEKIYIKRGCLPVALDEIKNVLGKKRVFIVTDNFLY
                     KNGLIKPITDKLDEMGVVYTTFFDVEPDPTLACAREGAKAMRDFEPDCIIAVGGGSPM
                     DAAKIMWVLYEHPDADFEDMAMRFADIRKRIYTFPKMGEKAYFIAIPTTAGTGSEVTP
                     FAVITDEKTGVKYPLADYELLPDMAIVDSNLMMNMPKSLTAASGIDVLTHAVEAYASM
                     LATEYTDGPALKAIKMVFEYLPRAYKNGGSDPEAIDKMANAATLAGMAFANAFLGICH
                     SLAHKLGAYFHLPHGVANALMINEVIRFNASETPVKMGTFPQYDHPNALKRYAEIADF
                     VGLGGKNDEEKLERLIEKIAELKEAVGIKKSIKEYGIEEEEFLNKLDEMSEQAFDDQC
                     TGANPRYPLIAELKELYLNAYYGK"
BASE COUNT          887 a          966 c          952 g         1038 t
ORIGIN      
        1 cagctttccg acacagccgt aagaatgggc aagaaagcta aaatccatgt ggttgtggat
       61 acgggaatgt cccgaatagg ttttccgccc accgaagaaa gcgttgaaac aatcaagtgg
      121 ataaacacct taccgggtct ctttgttgaa ggcatattca gccattttgc tcttgccgat
      181 tcaaaggata aaacggtcga caaaatgcag cttaaaaggt tcggctttgt ccttgacggt
      241 ctggagagag agggaataca tattcccata aagcatatat ccaactccgc cgcggtaata
      301 gactccgact acagctttga catggtgcgc tgcggaatta tgctctacgg catgtacccc
      361 tccgacgagg tggataaaac caaggtttca ttaaagccgg taatgtccct taaaagcagc
      421 gtaatacagg ttaagaccct gccccccggc gagggagtca gctacgggca cacctttgtg
      481 accacaaggg aaaccagaat cgccacagtc tccgcgggct acggcgacgg ctatccgaga
      541 gccctctcct caaaaggcag agtaattata aacggtcagt acgcccccat aatcggcaga
      601 gtctgcatgg atatttttat ggtggacatc agccatattg acggcgtaaa agtcgaggat
      661 cccgtaattc tcatcggcag ggacggggat aaggaaatca ccgccgagga ggtcggagcc
      721 cttgcggata ccttcaatta cgaaatagtc tgctccgtat cacgaagggt gccgagaatt
      781 taccttaaaa actctgtcga agtcggcgaa tactgcgcat tcaaataaaa aaggggatgg
      841 actttgagtc catcccttta aatttactgg aaatccagaa catttaccca ccttaaaagg
      901 aactcccttt cctcgggctt tccttttaca agctgagagg ccgccacaat gggaacccac
      961 ttctgcacat actgctttgc ggtgtcgctt ttaagacaga agagtttcag atattcctca
     1021 gccagctttt cgttgccgct gagccagaat aaaagatagg tccttgcggc gtctgccgag
     1081 gcgttgccct gcgttgcgtg tggccagtcc agaatatacg gcgtgccgtt gttgtggatg
     1141 attatattgc tggggttaaa gtcgccgtgg cagaccttgt tgtgggtggg cagagagttc
     1201 agccttgtat gcagctcata tcgggttgtt gcgtcaagcc cggattccga gatttttctg
     1261 ttcatcttat ccttgagctt gcccaaaagc ggcgctctgc gggagtgcat gtcaagctga
     1321 acatccacaa agagatttaa atattcattg agtttatcgg gattttcgtc cataagctgc
     1381 tgcaaggttt tgccgggaat gaagtcaaag acaatcgccc atttgccgtc aatcttcaaa
     1441 acctcttgaa ctttggggat attaagcccc gtttcctcaa cccttgcttg atttaacgac
     1501 tcattgagaa tatccgactt tttatagttt tcgtcaaaga ccttaattgc gtaatctccg
     1561 tctttgtaaa cggtcttgcc tgctcgaacc gcgataacat tgttaagatt catattcctc
     1621 gtttcctttc tgagctaagc taagtctgct tctggggtgc ggggagtatg cggcagcccg
     1681 tgccgcacac tccggggagg tagcatatct ttaaagaacg attacttgcc gtaataagca
     1741 ttaagataca gctctttaag ctctgctatc agcgggtatc tggggtttgc gcccgtgcac
     1801 tggtcgtcaa atgcctgctc gctcatctca tcgagcttat taaggaattc ttcttcctca
     1861 attccgtatt ccttaattga cttcttaatg cccacagcct ctttaagctc cgcgattttc
     1921 tcgatgagcc tttccagctt ttcctcgtcg ttctttccgc caagtcccac gaaatccgca
     1981 atctcagcgt atctcttaag ggcgttgggg tggtcatact gggggaatgt gcccatctta
     2041 acgggtgttt cgctggcgtt aaagcggatg acctcgttta tcataagagc gttggcgacg
     2101 ccgtggggaa ggtggaagta cgcgcccagc ttgtgagcca atgagtggca gattccgagg
     2161 aaagcgttcg caaatgccat gcccgccagt gtagcggcgt tagccatctt gtctatagcc
     2221 tcgggatcgc tgccgccgtt tttataagct ctcggcagat actcgaatac catctttatt
     2281 gccttgagag cgggcccgtc tgtatactcc gtcgccagca ttgaggcgta tgcctctacg
     2341 gcgtgagtca gaacatctat gcccgatgca gcagtaaggc tcttcggcat attcatcata
     2401 aggtttgaat cgactattgc catatcggga agcagctcat agtcagcaag gggatacttt
     2461 acgcccgtct tttcgtctgt gataaccgcg aagggtgtta cctcggagcc ggtacccgcc
     2521 gttgtgggga tggcgataaa gtaagctttt tcgcccatct tggggaaggt gtatatcctc
     2581 tttctaatgt cggcaaagcg catagccatg tcttcaaagt ctgcgtcggg atgttcgtaa
     2641 agtacccaca taatctttgc ggcgtccatc ggggagccgc cgccgacggc tataatgcag
     2701 tcgggctcaa agtctctcat ggcttttgcg ccttctcttg cgcatgcaag tgtcgggtcg
     2761 ggctcaacat caaagaaggt tgtgtataca acgcccattt catccagttt atccgtaatg
     2821 ggcttaataa gaccgttttt gtaaaggaag ttgtccgtta ctataaatac cctctttttg
     2881 ccgagaacat tcttaatttc atcgagggct acgggcaggc agccacgctt aatgtaaatc
     2941 ttttcgggcg ttctgaacca gagcatgttc tctctcctct cggcaacagt tttgatattt
     3001 ataaggtgct ttactcccac attctcggat accgagttgc cgccccatga gccgcagccg
     3061 agtgtcagcg agggagccag tttgaagttg taaaggtcgc cgatgccgcc gtgggaggag
     3121 ggtgtattaa tgagaactcg gcaggttttc atagcccttt cgaatttctc cgccttctcc
     3181 tttgcgttaa aggcgtcgac atacagcgag gatgtatgac ccagaccgcc gtcctcaata
     3241 agacgggccg ccttttcaat cgcctcgtca aaatccttgg ccttatacat cgcaaggacc
     3301 ggggagagct tttcgtgggc gaattcctcg gacagctcca cgcttgtaac ctcgccaatg
     3361 aggattttgg tgtctttggg aacctctatt cccgcaagct ctgcgattct gtatgcgggc
     3421 tgaccgacta tctttgcgtt gagcgcgccg tttatgatta ttgtctttct gactttatcc
     3481 agctcatcgc ccttgaggaa atagcagcct ctcttttcga actcgtcttt aacggccatg
     3541 tatatctttt ctggcacaat aacagcctgc tcagaggcgc agatcatgcc gttgtcaaag
     3601 gtctttgaat ggataatgga atttacagcg agcttaatgt ccgcgctttc atcgataacg
     3661 gcgggaacat tgccggcgcc cacgcctatt gcgggtttgc ccgatgagta tgcggccttt
     3721 acaaggctcg ggccgcctgt tgcaaggata atgtccgcct ctctcataac aaggtttgta
     3781 agctcaagag acggcacatc aatccatctg ataattccct ctggcgcgcc ggcttttacg
     3841 gca
//
LOCUS       DAONVQ010000146         3842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_16599_length_3842_cov_4.851862, whole genome shotgun sequence.
ACCESSION   DAONVQ010000146 DAONVQ010000000
VERSION     DAONVQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16599_length_3842_cov_4.851862"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..825
                     /locus_tag="PLD98_08180"
     CDS             250..825
                     /locus_tag="PLD98_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HQA06258.1"
                     /translation="MERKVLVETSARHVHLSREHLDILFGQGYELTPKKELSQPGQYA
                     CEERVAVVGPKGTFPSVAILGPVRNATQVEISATDARMLGIEAPVKESGDIAGSAGCK
                     IVGPKGEVEISEGVIIAKRHIHLDPETAEKFGVKDKQIVNVKVESDERSLIFGDVVVR
                     VHPNFSPAMHIDTDESNAVLAGNNVLGVILD"
     gene            898..1377
                     /locus_tag="PLD98_08185"
     CDS             898..1377
                     /locus_tag="PLD98_08185"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQA06259.1"
                     /translation="MSQPQQKLSKKDNIIQAIKFTLFSTSAGLIQLGSFTILERLTDL
                     AYWPKYLIALTLSVLWNFTLNRRFTFKSANNIPLAMLLVALYYVVFTPLSTWWGDALD
                     KIGWNEYLILAITMLANFVTEFLYQRFVVFRNSINTNKAAQRELQKKMQAEAQVDKT"
     gene            complement(1454..2131)
                     /locus_tag="PLD98_08190"
     CDS             complement(1454..2131)
                     /locus_tag="PLD98_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQA06260.1"
                     /translation="MSILDIGETGGSLTSKVFTYLESEILSGNLAPGTVLTESKISKE
                     LGVSRTPVREAIFQLEQEGLVKLSQNKSAVVVGISQKDIDDIYTIRLLIEGLASKWAA
                     ERISDEEIKKLEEVLELETFYADRGDIAKSRKLDTEFHSILYDASGSNPLKNTLKTFH
                     NYIGRAREISFASNNRALIAAKEHAEILKAIKNRDGEEAERLTREHIQNARANLYLDM
                     LKDKDSV"
     gene            complement(2159..3526)
                     /locus_tag="PLD98_08195"
     CDS             complement(2159..3526)
                     /locus_tag="PLD98_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQA06261.1"
                     /translation="MLELSKQTHLLERDPYRYRLQEVQEPELFREIYPYTDVPRVVFN
                     HRRVDLAMPEEIWITDTSFRDGQQSQSPYKVEHMVELYKLMHRLGGEKGIIRQTEFFI
                     YSENDRKAIEKCMELGYRFPEITTWIRANKKDFELARSMGIKETGILVSCSDYHIFKK
                     LKMTRSQALEHYLSVVAEAFDAGVIPRCHFEDITRADFYGFVVPFANALMDMSKEAGI
                     PVKIRVCDTLGLGIPYPGVALPRSVPGIINGLRHYAGVPSEQLEWHGHNDFYKAVVNA
                     GTAWLYGASAVNCSLLGIGERTGNVPLEAMVMEYISLRGSHDGMDTTAITDIARYFEE
                     KMGYQIPPMTPFVGRDFNQTRAGIHADGLMKDEEIYNIFDTKTILNRSAGVVVNNTSG
                     LAGIAYWINNRYNLTGERAVSKQSELVKKIKEWVDQQYQNGRETVISNDELEALIKEY
                     APDLI"
BASE COUNT          901 a          910 c          934 g         1097 t
ORIGIN      
        1 aggggtaaaa ccgattctcg gctctaaaag gttttatgct tataatccat aggcactcgg
       61 ttcaatcaca cgaaattgtg tatatattcc tttcaaaata cattcaattg cgcaatcttg
      121 cttgacttaa ttttaacaat ggtgtagaat gtaagagtaa attatcaaat cctttgcctt
      181 tagggtacag tgaaaagctg tgcccggaaa attttgtgat ttttcatttt atatttggag
      241 gataaaaaaa tggaaagaaa agtgcttgtt gaaacttcgg caagacatgt gcatctttca
      301 agggaacacc ttgatatctt attcggacag ggctatgagc tcacccccaa aaaggagctt
      361 tctcagcccg gtcagtacgc ttgtgaggag agggttgccg ttgtcggacc gaagggcact
      421 tttccctctg tagcaattct gggacccgtt aggaacgcta ctcaggttga gatttccgcc
      481 acggacgcaa ggatgctggg cattgaggct cccgtcaagg agtcgggcga catcgccggc
      541 agcgctggct gcaagattgt cggtcccaaa ggcgaagttg agatttcaga gggcgttatt
      601 atcgcaaaga ggcatattca ccttgacccc gaaaccgctg agaagttcgg cgttaaggat
      661 aagcagatag taaatgttaa ggttgagagc gatgagcgtt cgctcatctt cggcgatgtt
      721 gtcgtaagag ttcaccctaa cttctccccc gccatgcaca ttgataccga cgagtccaat
      781 gccgttttgg cgggaaacaa tgtcctcggc gttattttag attagttttg tgcctgtgct
      841 ttttaagcgc aggcatttat tatatcttgc ggcataatta atataggagg agtttttgtg
      901 tcacagcctc agcagaagct ttcaaaaaag gacaatatca ttcaggcaat taagtttacc
      961 cttttttcca cctccgcggg ccttattcag ctcggcagct ttacaattct tgagcgtctg
     1021 acggatttgg cttactggcc caaatacctt attgccctga ccctttcggt gctgtggaac
     1081 tttacattaa accgcagatt cacctttaag tccgcaaaca acatacccct tgccatgctg
     1141 ctggttgccc tttattatgt tgtattcact cccctttcca cttggtgggg cgacgccctt
     1201 gacaaaatcg gctggaacga gtatctgatt ctggcgatta cgatgctggc taactttgtg
     1261 accgagtttt tgtatcagcg ttttgtggtt ttcagaaact ccataaacac aaacaaggcg
     1321 gcgcaaaggg agctgcagaa aaagatgcag gcagaagcgc aagtggacaa aacttaaccg
     1381 gtaagagttg cgtaatgcag ccataaactc acgccaaagg caaaacccga tggaaaacca
     1441 tcgggttttg cttttatacg gagtccttgt ccttaagcat atctaagtat aagtttgctc
     1501 tcgcgttttg aatgtgctct cttgtaagac gctccgcctc ttctccgtca cggtttttta
     1561 tagccttcag gatttcggcg tgctccttgg cggcaattaa tgccctgttg ttgcttgcaa
     1621 aggagatttc ccttgccctg cctatgtagt tgtggaaggt tttaagcgtg ttcttgaggg
     1681 gatttgagcc gctggcgtcg tagagtatgc tatggaactc ggtgtccagc tttctgcttt
     1741 tagcgatgtc tcctctatcg gcatagaagg tctccagctc gaggacttct tccagtttct
     1801 ttatctcctc gtcgctgatt cgctccgctg cccattttga ggcaagacct tcaatcagga
     1861 gccttatggt gtagatgtcg tctatatcct tttgggatat gcccactacc accgcgcttt
     1921 tgttttggga cagctttaca agaccctcct gctcaagctg gaaaattgcc tctcttacgg
     1981 gagtgcggct gacgcccagc tcctttgaga ttttgctttc ggtcaaaacg gtgccgggtg
     2041 ccagatttcc tgagagaatt tcgctttcaa ggtatgtaaa tacctttgag gtcaaggaac
     2101 cgccggtttc gccaatgtcg agtatgctca tttttatccc cccaaacagt gaacggtttt
     2161 aaatgaggtc gggcgcgtac tcctttatga gagcttcaag ctcgtcgttg cttatgacgg
     2221 tttctctgcc gttttgatac tgctggtcaa cccattcttt gattttcttt acaagctcgc
     2281 tctgctttga aacagccctt tctcctgtta aattatacct attgttaatc caatatgcaa
     2341 tacccgcaag tccggaggtg ttgttgacca ccacgcccgc gctgcggttt agtatggttt
     2401 ttgtgtcaaa gatattgtag atttcctcgt ccttcataag accgtcggcg tggatgcctg
     2461 cccttgtctg gttgaaatct ctgcccacaa agggagtcat gggcggaatc tgatagccca
     2521 ttttctcctc aaaatatctc gcaatgtcgg taatggcggt tgtgtccatg ccgtcatggc
     2581 tgcctctgag cgaaatatac tccatgacca tagcctcaag ggggacattg cccgttctct
     2641 cgccaatgcc gagaagtgag cagttgaccg cgctggcgcc gtagagccat gcggtgcccg
     2701 cgtttacaac agccttatag aagtcgttat gtccgtgcca ctcaagctgc tcgctgggaa
     2761 cgcccgcata gtgcctgaga ccgtttatga tgcccggcac gctcctgggc agggctactc
     2821 ccgggtaggg tatgccgagg cccagggtat cgcaaaccct gatttttacg gggatgcccg
     2881 cttcctttga catatccata agggcattcg caaagggtac gacaaagccg tagaagtcag
     2941 cccttgtaat gtcctcaaaa tggcagcggg gaattacgcc cgcgtcaaag gcttcggcta
     3001 caacggagag atagtgctcc agagcctgtg agcgggtcat cttcagcttt ttgaaaatgt
     3061 ggtagtcgga gcaggaaaca aggattcccg tctccttaat tcccatggag cgggcaagtt
     3121 caaagtcctt tttgtttgcc cttatccatg tggtgatttc ggggaacctg tagccaagct
     3181 ccatacattt ttcaattgcc ttgcggtcgt tctcgctgta tatgaaaaac tcagtctggc
     3241 ggattatgcc cttttcgccg ccaagtctgt gcataagctt gtagagctcc accatgtgct
     3301 ccaccttgta gggggactgg gactgctgac cgtctctgaa agaggtgtcg gttatccaaa
     3361 tctcctcggg catggcaagg tcaaccctgc ggtgattgaa aacaacccgc gggacatccg
     3421 tgtagggata gatttctctg aaaagctccg gctcttgaac ttcctggagc ctgtacctat
     3481 aggggtctct ttcaagcaaa tgcgtctgtt tgctgagttc aagcatacat aaacaccttc
     3541 tgaaactgta ttattgtata attgtataca ataatacaat attgttgata atactatata
     3601 cttttgccgg agatttgtca acagaaattg tgaaaaaaat ctcaaagatt agaaaaactg
     3661 tataattagc caaagacagc cccatagaaa aaaggttttt tgtacgaacg gcagagagtt
     3721 ccatagccgc aaaagcttgt cctatcaagc cttggcgggg tgggacataa tttttatgct
     3781 gaccgctccc gtcggtgggg cgcgccctgc gtggcgggtt tggtcgggcg agcctttcag
     3841 tt
//
LOCUS       DAONVQ010000147         3747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_17116_length_3747_cov_4.700975, whole genome shotgun sequence.
ACCESSION   DAONVQ010000147 DAONVQ010000000
VERSION     DAONVQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17116_length_3747_cov_4.700975"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLD98_08200"
     CDS             complement(<1..403)
                     /locus_tag="PLD98_08200"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQA06262.1"
                     /translation="MLTDIEIAQSAKLRPITEIAEKIGIDEEYLIPYGKYKAKIDLEY
                     LNTLKDKKDGKLILVTAINPTPAGEGKTTVTVSLGQAMQHIGKKAVIALREPSLGPVF
                     GIKGGAAGGGYSQVLPMEDINLHFTGDIHAVG"
     gene            complement(422..1657)
                     /locus_tag="PLD98_08205"
     CDS             complement(422..1657)
                     /locus_tag="PLD98_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06263.1"
                     /translation="MDKNIKVLSAILLMVATLLLITSCVGNGKNETTADETTTDEPAT
                     EPQTAVPVEAKPFTNFKDYLTQIDFSYSTIEIAEINSEDIYMAVPLSKSARKANAIVK
                     YNLETGKTRTVYTLPDKSTVISKLSVNDDCLLYVTSRQDGSRQRVYARNLKTGSTRLI
                     YSGAKADDAVCPVLYNQKVYWIEKRGENQPALCSYNIANGEKSEVLSLSSRRADVSLS
                     INEGRLLFIDTVSGTPVLVIYDILNAEKTIIPTGAYNIKDAMLTGRFLIYSEYDRYRL
                     VETDEENKENDEENEEEIEGDDEKETKAPQRGAWVTRILNLDALKYYSDEESEIFQGV
                     MRASADRFVAVMKNALRFYLSGEKRISYMFTLPERNITCFAPDSMGRFVVAGKHGEKS
                     EKSGSFVIIVNMNGYFEKV"
     gene            complement(1724..2392)
                     /locus_tag="PLD98_08210"
     CDS             complement(1724..2392)
                     /locus_tag="PLD98_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HQA06264.1"
                     /translation="MRLIRLKFNKVGKAKYISHLDLYRTFSRAFRRAGIPLWYTEGFN
                     PRPYINFALPLPLGAESVNDCMDIKMEGDFPLEEIVKRLNEVLPEDLKTVSCAEAVMS
                     VRDISFAEYSIVFDGEKVSLDDLKEKLSGDLRANKKVKSGRKKVEKEVDISEYVKDYS
                     LTQQDGDIILGITLPAGSSFNISPLLLIDALFGDENEGLSNMLYVKNIRRERLLTKSL
                     EEFK"
     gene            complement(2373..>3747)
                     /locus_tag="PLD98_08215"
     CDS             complement(2373..>3747)
                     /locus_tag="PLD98_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HQA06265.1"
                     /translation="DIFLPGEGEEVTHELIDLLKECKKEGVSKREFLRRAAKIEGMYV
                     PSLYKVEYNENGTIASVTPTETAPEKVKKRIISDLDSVYYPDKFVVPYVDTVHDRAVA
                     EIFRGCIRGCRFCQAGYIYRPIREKSAEKVYEQSRKLCDSTGYDEVSLCSLSTSDYTQ
                     IQKLLDEMLDWASSNMINVSLPSLRADNFPKELMEKLNEVRRSGLTFAPEAGTQRLRD
                     VINKNITEEEILSTSRKAFQGGWTAVKLYFMLGLPEETDEDIVGIANLAQKVVNEFYN
                     NPDKPKGKSVTVSVGVSNFVPKPFTPFQWEPFCSLDEIERKQKLLKESVRTKKASLSF
                     HNPKMSMLEAVFARGDRRLCSVLYTAWKSGCAFDAWDDSFDFDKWTSAFGECNIDPDF
                     YILRKRDFDEVLPWDHLDYGISKKFLIRENELAHKGSTSPHCREKCSACGANALNGGK
                     CDALNKA"
BASE COUNT          817 a          904 c          919 g         1107 t
ORIGIN      
        1 cgcccacagc atgaatgtcg cctgtaaaat gcagatttat atcctccatg ggcagaacct
       61 gagagtagcc gccgcccgct gctccgccct tgatgccgaa aacgggaccg agggagggct
      121 cacgcagggc tatgacagcc tttttgccta tgtgctgcat ggcttgtccc aggctgacgg
      181 taaccgttgt cttgccttca cccgcggggg tggggttaat ggcggttaca agtatcagct
      241 tgccgtcctt tttatccttt aaggtgttta aatattcaag gtcgattttg gccttgtatt
      301 ttccgtaggg aatcaaatac tcctcgtcaa tgccgatttt ttcggctatt tccgtgattg
      361 gccttaattt cgcgctctgg gctatctcaa tatccgtcag catttcggca cacctttcga
      421 gttagacttt ttcaaaatat ccgttcatat tgactattat aacaaagctg ccgctttttt
      481 cgcttttctc gccatgcttg cccgccacaa caaagcgtcc catggaatcg ggggcaaagc
      541 aggttatatt cctttcgggc agggtgaaca tgtaggaaat ccttttttct ccgctgagat
      601 agaagcgaag ggcgtttttc ataaccgcca caaagcggtc agccgatgcc ctcatgacgc
      661 cttggaaaat ctcggattcc tcgtcgctgt aatactttag ggcatcgaga ttcagaatcc
      721 ttgtgaccca tgctccgcgt tggggagctt tggtttcctt ctcgtcgtcc ccttcgattt
      781 cttcctcgtt ttcctcgtcg ttctccttat tctcctcgtc ggtttcgact aatctgtatc
      841 tgtcatactc ggagtagata aggaatctgc cggtgagcat ggcgtccttt atattgtacg
      901 cgcctgtggg gataatggtc ttttcggcgt tgagaatgtc gtaaatcaca agaacggggg
      961 tgccggagac cgtgtcgata aagagtagcc tgccctcgtt tatagaaagg ctgacatcgg
     1021 ctctgcggct gcttaaagag agaacctccg atttttcgcc gtttgcgata ttgtaagagc
     1081 aaagagcggg ctgattctcg ccccgttttt caatccagta aaccttctga ttgtaaagca
     1141 cgggacaaac ggcgtcatcg gctttagcac cggagtatat aagccttgtg ctgcctgttt
     1201 tgagattcct tgcataaacc ctttggcgcg aaccgtcctg tcttgaggtc acatacaaaa
     1261 ggcagtcgtc atttacgctg agctttgaaa tgacggtgct tttgtcggga agggtgtaga
     1321 cggtccttgt ctttcccgtt tcgagattat actttacaat ggcgtttgcc ttgcgggcac
     1381 tttttgaaag agggaccgcc atgtaaatgt cttcggagtt tatctctgca atctctattg
     1441 tagagtagga aaaatctatt tgagtcaaat aatctttgaa attggtgaag ggctttgcct
     1501 caacgggaac ggctgtctgt ggctcggtgg cgggctcgtc tgtggtggtt tcatcggcgg
     1561 tggtttcgtt cttgccgttt ccgacgcatg aggtaattaa taggagcgtg gcaaccataa
     1621 gcaggattgc agataaaacc tttatatttt tatccatatt tatctccgtt aagaaaacat
     1681 atagttgatt atattttcag agcggtattt tgtcaacaag gcactatttg aactcctcaa
     1741 ggctcttggt taaaagcctt tcgcggcgta tgttttttac atagagcata ttggaaagcc
     1801 cctcgttttc atctccgaaa agggcgtcaa tcaaaagcag ggggctgata ttgaaactgc
     1861 tgccggcggg aagggtgatg ccgaggatta tgtcgccgtc ttgctgagtc agcgaatagt
     1921 cctttacata ttcggatatg tcgacttcct tttcaacctt tttcctgccc gatttaacct
     1981 ttttatttgc tcttaaatcc ccgctgagct tttcctttaa atcgtcaagg cttacctttt
     2041 cgccgtcaaa gacaatgctg tactctgcaa agctaatatc ccttacggac atgacagcct
     2101 cagcgcagga tactgtcttt aaatcttcgg gcagaacttc attcagcctt ttaactattt
     2161 cttcaagggg gaaatcgccc tccattttta tgtccatgca gtcgtttacg ctctcggcgc
     2221 cgagcggcag cggcagggcg aaattgatat aggggcgtgg attaaaaccc tcagtatacc
     2281 aaaggggtat gcccgcacgc ctgaaggctc ttgaaaaggt gcggtaaagg tccaggtggg
     2341 agatgtattt tgcctttccc actttattga atttaagcct tattaagcgc atcgcatttt
     2401 cctccgttga gagcattggc accgcaggct gagcattttt ctctgcagtg ggggcttgtg
     2461 ctgcctttat gagcaagctc attttctctt ataaggaact tctttgaaat gccgtagtca
     2521 aggtggtccc aggggagaac ctcgtcaaaa tccctctttc gtaagatgta aaaatcgggg
     2581 tcaatgttgc attctccgaa cgcacttgtc cacttgtcaa aatcaaagct gtcgtcccac
     2641 gcgtcaaagg cgcagcccga tttccaggcg gtgtaaagca cggagcaaag ccgcctgtcg
     2701 ccccttgcga aaacagcctc aagcatgctc attttgggat tgtgaaagct gagacttgcc
     2761 tttttggttc tgacggattc ctttagaagc ttttgctttc tttcgatttc atccagtgag
     2821 cagaagggct cccactggaa gggggtgaag ggcttgggga cgaagttgga aacgcccacg
     2881 ctgacggtga cgcttttgcc cttgggcttg tcggggttat tgtagaactc gtttacaacc
     2941 ttttgggcaa ggttcgctat accgactata tcctcatcgg tctcctcggg cagacccagc
     3001 ataaagtaga gcttgaccgc cgtccagccg ccctggaacg cctttctgga ggtgctgagg
     3061 atttcctcct cggtgatatt tttgtttatt acatctctga gcctttgggt gcccgcctcg
     3121 ggagcaaagg taagtccgct gcggcggact tcattgagct tttccataag ctctttcggg
     3181 aagttgtccg cccttaaaga aggaagggac acattaatca tattgctgct tgcccaatcc
     3241 agcatttcgt caaggagctt ttgaatctgg gtatagtcgc tggtggaaag ggagcaaagc
     3301 gaaacctcgt catagcctgt gctgtcgcaa agcttacggc tttgctcata gaccttttcg
     3361 gcgctctttt cccttatggg tctgtaaatg tagccggcct ggcagaaacg gcagcccctt
     3421 atgcagccgc ggaaaatctc cgccactgcc ctgtcgtgaa cggtgtccac atagggaact
     3481 acaaatttgt cgggatagta aactgagtca aggtcagaaa ttatcctctt tttaaccttt
     3541 tctggggctg tttctgtggg tgtgacggag gctatggtgc cgttctcatt gtactctacc
     3601 ttatagagcg acgggacata cataccctct attttagcgg ctcttcttaa aaactcccgc
     3661 tttgatacgc cctctttttt gcactctttt aataggtcaa taagctcatg ggtgacctcc
     3721 tccccctctc cgggaaggaa aatgtct
//
LOCUS       DAONVQ010000148         3732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_17203_length_3732_cov_4.364156, whole genome shotgun sequence.
ACCESSION   DAONVQ010000148 DAONVQ010000000
VERSION     DAONVQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17203_length_3732_cov_4.364156"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /gene="dapA"
                     /locus_tag="PLD98_08220"
     CDS             complement(<1..493)
                     /gene="dapA"
                     /locus_tag="PLD98_08220"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HQA06266.1"
                     /translation="MKDLLFTGSGVAIVTPFTEDGREIDFPMLEKLINFQIENHTDAI
                     IICGTTGEGSTLTHEEHAAAIDFAVKVVAGRVPVIAGTGSNDTEYAVKLSNEAERSGV
                     DGLLMVTPYYNKTSQDGLIEHYNYIADRVSTPIILYNVPSRTGLDIKPSTYLELSKHK
                     RIVA"
     gene            complement(529..1620)
                     /gene="asd"
                     /locus_tag="PLD98_08225"
     CDS             complement(529..1620)
                     /gene="asd"
                     /locus_tag="PLD98_08225"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQA06267.1"
                     /translation="MKKYKVGIIGATGMVGQRFALILANHPWYEVTVLAASPRSAGKT
                     YKEALGNRWVMEEALPENLADIVVMDATADIEKIAKSVDFVFCAVDMPKEEIKALEEA
                     YAKAECPVMSNNSAHRFTEDVPMLIPELNHEHAAVIEAQKKRLGTKRGFIAVKSNCSL
                     QSYVPALYPVDKKFKVKDVLVCTYQAISGAGKTFETWPEMVDNVIPYIGGEEEKSEKE
                     PLKIWGKVENGKIVNASEPNFTAQCIRVPVTNGHLAAVFVRFENKPSKEEILEIWKNF
                     KGRPQELELPSAPKQFLHYFEEDNMPQTKLHRNLENGMAVSLGRLREDTQYDYKFICL
                     SHNTLRGAAGGAVLMAELLCAEGYITPRD"
     gene            1811..2836
                     /gene="queA"
                     /locus_tag="PLD98_08230"
     CDS             1811..2836
                     /gene="queA"
                     /locus_tag="PLD98_08230"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQA06268.1"
                     /translation="MKKSDFYFDLPEELIAQEPPKNRTDSRLLKLDRRTGEITHTRFP
                     ELINYLNPGDCLVLNDTRVLPARLIGTKIPTGAHVEFLLLKNMGNDVWEAMAGPARRA
                     KKGSEFTFGNGIMRGVVEEELDNGNRLIKLIYDGIFLEVLERIGEIPLPPYIKKKLED
                     SERYQTVYSKKLGSAAAPTAGLHFTEEMLREIEKKGVKLAYITLHIGIGTFRPVKAEN
                     IEDHKMHFESYTISEKAAEIINSTRDNGGRVISVGTTSCRTLESNSDERGYVTPCSAE
                     TDIFIYPGYKFKCIDGLITNFHLPESTLIMLVAAFCGRENTLRAYNEAVKERYRFFSF
                     GDAMFII"
     gene            2947..>3732
                     /gene="tgt"
                     /locus_tag="PLD98_08235"
     CDS             2947..>3732
                     /gene="tgt"
                     /locus_tag="PLD98_08235"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQA06269.1"
                     /translation="MFKLLKQCNTARRGEFTTAHGTVQTPAFMNVATAAAIKGGLSAY
                     DLKDLKCQVMLCNTYHLHLRPGDEIVKAFGGLHKFTGWDGTILTDSGGFQVFSLAKLR
                     DIKEEGVSFNSHIDGRRIFIGPEESMTIQANLGSDIAMAFDECVENPAPYDYAKLSCD
                     RTTRWLIRCLEKHNELIARPDAVNKKQMLFGINQGSVFDDLRAKHMEEIAKLDLDGYA
                     IGGLAVGEPKEVMYRIISVVEPFMPKDKPRYLMGVGTPENILEG"
BASE COUNT          995 a          890 c          883 g          964 t
ORIGIN      
        1 ccgccactat tctcttatgc tttgaaagct caagatatgt tgagggctta atgtctaaac
       61 ccgttctgct ggggacatta tataatataa tcggtgtcga aactctgtcg gctatgtagt
      121 tgtagtgctc tataagaccg tcctgagagg tcttgttgta ataaggagta accattaaca
      181 gtccgtcaac tccggagcgc tcggcctcgt tggagagctt tacggcatac tctgtgtcgt
      241 tgctgccggt gcccgcgata acgggaactc tgcccgcaac tacttttacc gcaaagtcga
      301 tggccgcggc gtgctcctca tgggtaagcg ttgagccttc tcctgttgtg ccgcaaataa
      361 tgattgcgtc ggtatggttt tcaatctgga agtttataag tttctcaagc atgggaaaat
      421 caatttccct gccgtcttcg gtaaagggtg taacaattgc aacgcccgaa ccggtaaaca
      481 gtaagtcctt cataaaaatt actcctttta ttaaaggtag cttaaaggtt aatcgcgggg
      541 agtgatgtag ccctcagcgc aaagcagctc agccataaga accgcgccgc ccgcagcgcc
      601 tctgagtgtg ttgtgggaca ggcagatgaa cttatagtca tactgagtgt cctcgcgcag
      661 tctgccgagt gacacagcca tgccgttctc aagattcctg tgcagctttg tctgaggcat
      721 attgtcctcc tcaaagtaat ggaggaactg tttgggagcg gaggggagct ccagctcctg
      781 gggcctgccc ttgaagtttt tccatatctc aaggatttct tcttttgagg gcttgttttc
      841 aaaccttaca aacacagcgg caaggtgacc gtttgtaacg ggaactctta tgcactgtgc
      901 tgtaaagttc ggctcggatg cattaacaat ctttccgttt tcaaccttac cccaaatctt
      961 gaggggctct ttttcagact tctcttcttc tccgccgatg taggggatta cattgtccac
     1021 catctcgggc catgtttcaa atgtcttgcc tgcgcctgaa attgcctgat aggtgcagac
     1081 cagaacatcc ttaaccttaa acttcttgtc aacgggatag agggcgggaa catagctctg
     1141 aagcgagcag ttggatttaa ccgcaataaa gccgcgcttt gttccaagac gctttttctg
     1201 tgcctctata accgcagcat gctcatggtt cagctcgggg ataagcatgg gcacatcctc
     1261 tgtgaacctg tgagcgctgt tgttggacat aacggggcac tctgcctttg cgtatgcctc
     1321 ctcaagagcc ttaatctcct ctttgggcat atcgactgcg cagaatacaa agtccacgct
     1381 ctttgcgatt ttctctatat ctgccgttgc gtccataact acgatgtccg ccaagttttc
     1441 aggcagagcc tcttccataa cccatctatt tccaagcgcc tctttgtagg tcttgcccgc
     1501 ggatctgggg ctcgccgcca gtacggttac ctcataccaa gggtggtttg cgagtatcag
     1561 agcaaacctc tgacctacca ttcctgttgc gcctataatg cctactttgt actttttcat
     1621 atgtatcctc cgacttgaca aataaattgc tattgttttt aatatgtttc tgcaatataa
     1681 tattcaagta tgcgatggaa aaataaaatt cttccccaat ttaaagttta attattttat
     1741 ataataacac ttgaactcgt tgaagtcaaa cgattttaga gctgagtcaa aattttttgg
     1801 agtaattttt atgaaaaaaa gcgattttta ttttgatctt cccgaagagc tgattgccca
     1861 ggagcctccc aaaaacagaa ccgattcaag actattaaaa ctggacagaa gaacggggga
     1921 aattacccac accagattcc ccgagcttat aaactatctt aatccggggg attgtcttgt
     1981 tctcaacgac acaagggtgc tgcccgcaag gcttatcggc acaaaaatcc ccacaggcgc
     2041 ccatgtggag tttctgcttc tgaagaatat gggtaacgat gtctgggagg ctatggcggg
     2101 tcccgcaaga agagctaaga agggcagcga gtttactttc ggcaacggca taatgcgcgg
     2161 agtcgtggaa gaggagcttg acaacggcaa cagactcata aagctcatat atgacggcat
     2221 tttccttgag gtgctggaga gaatcggcga aattccgctg ccgccttata tcaagaaaaa
     2281 gctggaggac agcgaaaggt atcagaccgt ttactcaaag aagctgggct ccgcggcggc
     2341 tcctactgcg ggtctgcact ttaccgagga aatgctgagg gaaatcgaaa agaagggagt
     2401 aaagctggct tatattactc ttcatatcgg catcggaacc ttcagacccg tcaaagcgga
     2461 gaatatagag gaccataaaa tgcactttga gagctacact atcagcgaaa aagctgccga
     2521 aatcataaac tccacaaggg ataacggcgg cagggtaatc tcagtcggaa caacctcctg
     2581 cagaaccctt gagagcaaca gcgacgaaag aggctatgta accccctgca gcgcggaaac
     2641 ggatattttc atatatcccg gttataagtt caagtgcata gacggtctaa tcaccaattt
     2701 ccacctgccc gaaagcaccc tcattatgct tgtagccgcc ttctgcggca gggaaaacac
     2761 cctaagagct tataacgagg ctgtaaaaga acgctacagg ttcttctctt tcggcgacgc
     2821 gatgtttatc atttaataaa atttactttc taatttttaa gtttattttt tcgcccgtta
     2881 ttaatttatg caatacgggc ttgacaggac tctatttaat aaaagcaaac aaaagttggt
     2941 gcctaaatgt ttaaactgtt gaaacaatgc aatacagcaa gaaggggcga gtttaccaca
     3001 gcccacggaa cggttcagac gcctgctttt atgaatgtcg ccacagcggc agctataaaa
     3061 ggcgggctgt ccgcctatga cttaaaggac cttaaatgtc aggtaatgct ttgcaatacc
     3121 tatcacctgc atttaagacc cggcgatgaa atcgtcaaag ccttcggagg acttcataag
     3181 ttcacaggct gggacggaac aatccttacc gacagcggcg gctttcaggt cttttccctg
     3241 gcaaagctga gggatattaa ggaggaggga gtcagcttca actcccatat agacggcaga
     3301 agaattttca tcgggcctga ggagagcatg accattcagg caaacctggg ctccgacata
     3361 gccatggcgt ttgatgaatg cgtagaaaac cccgccccat acgattacgc taagctttcc
     3421 tgcgatagaa caacccgctg gcttataaga tgcctcgaaa agcacaacga gctgattgca
     3481 agacccgacg cggtcaacaa aaagcagatg cttttcggta taaaccaagg ctccgtcttt
     3541 gacgatttaa gggcaaagca catggaggaa atagcaaagc tggaccttga cggctacgca
     3601 atcggcggac ttgcggtggg cgagcccaaa gaggtaatgt ataggataat ttccgtcgtt
     3661 gagcccttca tgcccaagga caagcccaga tacctcatgg gcgtgggcac tcccgaaaac
     3721 atcctggagg ga
//
LOCUS       DAONVQ010000149         3701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_17384_length_3701_cov_5.262754, whole genome shotgun sequence.
ACCESSION   DAONVQ010000149 DAONVQ010000000
VERSION     DAONVQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17384_length_3701_cov_5.262754"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLD98_08240"
     CDS             complement(<1..421)
                     /locus_tag="PLD98_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HQA06270.1"
                     /translation="MQTVNVKDFGAMSDGINDDFSAFQKALGAGERKVYIPAGRYRIS
                     KTLKVACNTEIIADDNAEIFVCEKLPKKRGDFLLTNAEHEKGNKNITIKGGVWSGNYD
                     GKNNSKHPDIFEPTAASGALINFFNVENLKIEGVTLAN"
     gene            complement(671..1210)
                     /locus_tag="PLD98_08245"
     CDS             complement(671..1210)
                     /locus_tag="PLD98_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017895450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HQA06271.1"
                     /translation="MLLVSEVYTTPPAEFKTELQEMTYKVLKELDIPFERVDTEEAIT
                     MEDCLNINRALNVKVVKTLFLCNRQQTAFYLFITEGDKPFVTKDFSKALGISRVSFAP
                     AELLYEMLGTKVGATTVFSMLLDRENKVQVVIDKDVLKEEWYGCTDGTTTCYMRVKTE
                     RILNDFLPYTKHEPKFIEV"
     gene            complement(1277..1471)
                     /locus_tag="PLD98_08250"
     CDS             complement(1277..1471)
                     /locus_tag="PLD98_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06272.1"
                     /translation="MSEFTKEELCEAKRAIESTIRKCEKVLPKLREGTSQHTLLVRRI
                     KAFQIAVELINAELENQSPY"
     gene            complement(1515..1928)
                     /locus_tag="PLD98_08255"
     CDS             complement(1515..1928)
                     /locus_tag="PLD98_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQA06273.1"
                     /translation="MWKCPKCGREFKNENQRHFCGDPSKMTVDDYINSQPEEIIPILN
                     KVRETIRKALPDAEEKISYGMPTYKEKHNLIHFAAQKKHLGLYPGEKAVEHFEDRLSD
                     YKTSKGAVQFPYNKEIPYELIAEIAKWRKETGNHA"
     gene            complement(2043..>3701)
                     /gene="asnB"
                     /locus_tag="PLD98_08260"
     CDS             complement(2043..>3701)
                     /gene="asnB"
                     /locus_tag="PLD98_08260"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQA06274.1"
                     /translation="PMTIERGCESYTIVYNGELYNTEDLRKELLSAGYSFRGHSDTEV
                     LLTAFIHWGEDCLQKLNGIYAFAVWDEKNRRLTLARDRMGVKPLFYSVIPRGLIFGSE
                     IKTLLANPLIKPRLDNDGLKEIFLLGPGRTGGNGVIKGIEELKPAEFMVFEGDRVTKK
                     AKYWNLRAVEHTEDIKGSVEKVRFLITDAVKRQLVSDVPLCCFLSGGLDSSIISKIAS
                     DDYKATGKAPLNTYSVNYVDNKRFFKKSLFQPNSDEEYIRMMSEHIGSEHREVLLDNE
                     DLAVYLDEAAIARDLPGMADVDSSLLMFCREIKKDFTVAVSGECADEIFGGYPWYHNR
                     EILYRDTFPWSQSLDIRFSLLKQGLIKDGEEYVRQRYLDTVNEADTLPGDDELEKRMR
                     QMFMLNLNWFMQTLLDRKDRMSMFCGLEVRVPFCDHRLVEYAYNMPWSLKSLNGREKG
                     VVREAMKGILPDEIIERKKSPYPKTHNPKYLSAVKNRLQRIIDDPSSPLLQLVDKQKV
                     QEIIDSDGAALTSPWYGQLMTGPQLMAYLIQVDVWMTEYKIELV"
BASE COUNT          863 a          905 c          828 g         1105 t
ORIGIN      
        1 ggtttgcgag agtcacgcct tcgattttta aattctccac attaaaaaaa tttatcaaag
       61 ccccgcttgc cgcagtgggc tcaaagatgt cggggtgctt tgagttgttt ttgccgtcgt
      121 agttgccgct ccatacgccg cctttgatgg tgatattctt attgcccttt tcatgctcgg
      181 cgtttgtgag aagaaaatcg cccctctttt tgggcagctt ttcgcacaca aaaatttcgg
      241 cgttatcatc ggcaattatt tctgtattgc aggcaacctt taaagtcttt gaaatgcggt
      301 acctgccggc gggaatataa actttccgct cccctgcgcc cagcgccttt tgaaatgctg
      361 agaagtcgtc atttatgccg tcgctcatag cgccgaaatc tttgacattt accgtttgca
      421 ttttagtacc cctttaaata gtttggatat attagactaa ttttttaagg aaattcaaca
      481 attcatgtat aatataatat attttacggg gcaaatgttt caaaactgta aatttatgag
      541 atttcacata aagtggtttt taagacattt agttctttat ttcatatgta ctttcgcggc
      601 gtaatatata ttctgttttg gcccttttta caaaaaaaac agcagcagag cgtaaaactc
      661 tgctgcctta ttaaacctct ataaactttg gctcgtgctt tgtgtagggc aggaagtcgt
      721 taaggattct ctccgtcttg accctcatat agcaggtggt cgtgccgtcg gtacagccgt
      781 accattcctc tttgagaaca tccttgtcta tgactacctg aaccttgttt tctctgtcaa
      841 gaagcatgct gaaaaccgtt gtggcgccaa cctttgtgcc gagcatttcg taaaggagct
      901 ccgcgggcgc gaaggatacg cgggaaattc ccaatgcctt gctgaaatcc tttgttacaa
      961 agggcttgtc cccctcggtg ataaaaagat aaaaggctgt ctgctggcgg ttgcacaaaa
     1021 acaaagtctt tacaaccttt acattcagag ccctgtttat gttaaggcag tcctccattg
     1081 tgattgcctc ttcggtatcc acccgctcaa agggaatatc aagctcttta agtaccttgt
     1141 atgtcatctc ctgcagttca gttttaaact cggcaggggg cgtggtgtaa acttcactta
     1201 caagaagcat tgcaatctcc tttcggcttt tttaaagaca ctatacaact aaaagctcgg
     1261 aatttcaaga gagaccttag tagggcgact gattttcaag ctcggcgttt ataagctcga
     1321 ccgcaatttg aaaggctttg attctgcgga caagcagggt gtgctgggat gtgccttccc
     1381 taagttttgg cagaaccttt tcgcacttgc ggatggtgga ttctatggct cttttagcct
     1441 cgcatagttc ttcctttgta aactcgctca tatattctcc tttcgatagc cacggctgag
     1501 tgtgatgacc tagtttaagc gtgattgccc gtttccttgc gccattttgc tatttcggct
     1561 atgagctcat aggggatttc tttgttatac gggaactgca ccgcgccctt gcttgttttg
     1621 taatcggaaa gcctatcctc aaaatgctcc accgcctttt cgccggggta gagccccaaa
     1681 tgcttttttt gcgcggcgaa atggatgaga ttatgctttt ctttgtaagt gggcataccg
     1741 tatgagattt tctcctcggc gtcggggagg gctttacgga tggtttcccg cactttattc
     1801 agtataggta taatctcctc gggctgagag tttatatagt cgtccacggt catttttgaa
     1861 gggtcgccgc agaaatgtct ttgattctcg tttttaaact cccgtccaca ctttgggcac
     1921 ttccacatag atttcagtcc tttccgtaca agctttattt ctatcttact ttatccttgc
     1981 caaattgcaa taaaaatgtg cgggcaggct ttttgcccgc ccgcaatcgg caccgcattc
     2041 atctatacca attcaatctt atattctgtc atccaaacat ccacctgaat aagatacgcc
     2101 ataagctggg gaccagtcat gagctgaccg taccaggggg atgtgagcgc cgctccgtcg
     2161 gagtctataa tctcctgcac cttttgtttg tcaacaagct gcaaaagggg tgaggatggg
     2221 tcgtctatta ttctctgaag tctattcttt acggcgctga gatatttggg attgtgggtt
     2281 ttgggatagg ggcttttttt gcgctcgatt atttcgtcgg gcaaaatgcc cttcatagct
     2341 tccctgacaa ctcccttctc cctgccgttt aatgacttta agctccacgg catattgtag
     2401 gcgtactcca caagacggtg gtcgcagaag ggcaccctaa cctccagacc gcagaacata
     2461 ctcattctgt cctttctgtc aaggagggtc tgcatgaacc agttgagatt aagcataaac
     2521 atctgccgca tgcgcttttc aagttcgtcg tcgccgggca gggtgtcggc ttcgtttacc
     2581 gtatctaagt acctctgcct tacatattcc tcgccgtctt taatgagccc ttgctttaag
     2641 agactgaatc ttatgtcaag ggactgcgac cacgggaagg tatcccgata cagaatctcc
     2701 ctgttgtgat accagggata gccgccgaaa atctcgtcgg cgcactcgcc ggaaactgcg
     2761 acggtaaagt ccttttttat ctccctgcag aacataagaa gggaggagtc cacatccgcc
     2821 attccgggca ggtctcgggc aatagccgcc tcgtccaagt ataccgccaa atcctcgttg
     2881 tcaaggagta cctcgcggtg ctcggagccg atatgttctg acatcatcct tatatattcc
     2941 tcatcggaat tcggctgaaa gaggcttttc ttaaaaaagc gcttgttgtc aacatagttt
     3001 actgaataag tattgagcgg cgctttgccc gtcgccttgt aatcgtctga ggctatcttg
     3061 gaaataatgc tggagtccag accgccagaa aggaaacagc acagcggcac atcggacaca
     3121 agctgccttt ttacggcgtc ggtaataaga aaacgcacct tttcaacgga gccttttata
     3181 tcttccgtat gctcgaccgc cctgagattc cagtacttcg cctttttagt taccctgtca
     3241 ccctcaaaaa ccataaactc cgcgggcttt agctcctcaa tacccttaat gacgccgttg
     3301 ccgcccgttc tgccgggccc caagaggaaa atctctttaa ggccgtcatt gtcaagcctc
     3361 ggcttaatta aggggttggc aaggagagtc ttgatttctg agccgaaaat taagcccctc
     3421 ggtatgacgc tgtaaaaaag aggctttacg cccatacggt cacgcgcaag ggtcagacgc
     3481 cgattctttt cgtcccatac agcgaaggcg taaatgccgt taagtttctg aaggcagtcc
     3541 tcgccccagt ggataaaggc ggtgagcaga acctcggtgt ctgagtgacc tctgaagctg
     3601 tagcctgctg ataacagctc ctttctcaaa tcctcggtat tgtaaagctc gccgttatat
     3661 acaattgtat agctttcgca gcccctctct attgtcatag g
//
LOCUS       DAONVQ010000150         3692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_17439_length_3692_cov_4.290899, whole genome shotgun sequence.
ACCESSION   DAONVQ010000150 DAONVQ010000000
VERSION     DAONVQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17439_length_3692_cov_4.290899"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLD98_08265"
     CDS             complement(<1..309)
                     /locus_tag="PLD98_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06275.1"
                     /translation="MVYVIPSGVGSSVGSSEGSSEGSSEGSSEGSSEGSSVGSSEGSS
                     EGSSVGSSEGSSEGSSVGSSEGSSEGSSVGSSEGSSEGSSVGSSEGSSEGSSVGSSEG
                     S"
     gene            317..>557
                     /locus_tag="PLD98_08270"
     CDS             317..>557
                     /locus_tag="PLD98_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HQA06276.1"
                     /translation="MGDVDGDGKISAADARLALRHAAKLDSLEGPYFLAADMNRDGKV
                     LPNDARKILRIEAQLDELTVPTEEPTEEPSEEPTEE"
     assembly_gap    558..657
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(915..990)
                     /locus_tag="PLD98_08275"
     tRNA            complement(915..990)
                     /locus_tag="PLD98_08275"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(955..957),aa:Thr,seq:ggt)
     gene            complement(1225..1434)
                     /gene="copZ"
                     /locus_tag="PLD98_08280"
     CDS             complement(1225..1434)
                     /gene="copZ"
                     /locus_tag="PLD98_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HQA06277.1"
                     /translation="MTSVIMKVEGMSCEHCSAAITRAVKALDGVSKVEVDLKSKTVEV
                     EFDPAKLNADAIKAAIEDQGYDVLG"
     regulatory      1576..1705
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1769..2344
                     /locus_tag="PLD98_08285"
     CDS             1769..2344
                     /locus_tag="PLD98_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HQA06278.1"
                     /translation="MKKQLKMVQLSMLAALGVVLMLIVRFPLFPSAPFLEYDMGDVAV
                     ILTAFLYSPWASLLVLVIVSLIQALTVSASSGWIGFVMHVISTGAFVLVCGLIYNRKR
                     GAKSKVSKLYLPAALAAGTIAMVLVMIPLNLIFTVRFMQVPREAVLNMMIPTIIPFNV
                     LKGIINSVVVCVLYPAVKKILKSSKSLNPEL"
     gene            complement(2375..2725)
                     /locus_tag="PLD98_08290"
     CDS             complement(2375..2725)
                     /locus_tag="PLD98_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQA06279.1"
                     /translation="MAKKTEINCPVSATLKLIGGKYKSLILWHLMRGTMRYGEIQRLI
                     PEATPKMLTQQLRELENDGLLVRTVYPVVPPKVEYSLTELGRSLEPILIAMYDWGAEY
                     MKNNNIKICCSMER"
     gene            2862..>3692
                     /locus_tag="PLD98_08295"
     CDS             2862..>3692
                     /locus_tag="PLD98_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HQA06280.1"
                     /translation="MTNKTFNIKGMTCAACAQRIEKVAGRLDGVESAAVNLTAEKLFV
                     KYDEAKARPEDIIAAVTKIGYEAEEEKTSAVISLPISGMTCAACAQRIEKVLNRLDGV
                     YKASVNLAAEKATVEFDPSKIRTGAIKEAIEKLGYKVLEVKKESRVEEERERKRKEIK
                     RLWTKFIVAAVFAFPLLYIAMGPMISLPVPSFLDPDISPLNYALVQLILVIPVMAVGY
                     RFYTVGFKALINRSPNMDSLIAISTLAAFVFSVWNTYQIIKGNHHAAHSLYFETAGVI
                     I"
BASE COUNT          934 a          862 c          849 g          947 t
ORIGIN      
        1 tgagccctct gatgagccca ctgatgagcc ctctgatgag ccctctgatg agcccactga
       61 tgagccctct gatgagccct ctgatgagcc cactgatgag ccctctgatg agccctctga
      121 tgagcccact gatgagccct ctgatgagcc ctctgatgag cccactgatg agccctctga
      181 tgagccctct gatgagccca ctgatgagcc ctctgatgag ccctctgatg agccctctga
      241 tgagccctct gaggagccct ctgatgagcc caccgaggag cccacacccg agggaattac
      301 atacaccatt gttcttatgg gcgatgtgga cggcgacggc aagattagcg ccgctgacgc
      361 aagacttgct ctgcgccatg ccgcgaaact cgactccctt gagggaccgt atttcctcgc
      421 cgccgacatg aacagagacg gaaaggtcct ccccaatgac gcgaggaaga ttttaagaat
      481 cgaggcacag cttgacgaac ttactgttcc cacagaggag cccactgagg agccctctga
      541 ggagcccact gaggagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct
      661 ctgaggagcc ctctgaggag ccctctgagg agccctctga ggagccctct gaggagccct
      721 ctgaagaatc cacagaggaa tccacagagg aatccacaga ggaatccact gaggaatcca
      781 ctgaggaatc cactgaggaa ttcacagagg attctacaga ggcaaactaa tccataaaag
      841 cataaattta agggtttgag tcatgctcaa accctttttt acataaaaaa agaggcttga
      901 aaaactcaag cctttggagc tgttaaccgg gatcgaaccg gtgacctcat ccttaccaag
      961 gatgtgctct gcctactgag ctataacagc aatattcaat tgcccagcaa aagatattat
     1021 acacataaaa aatattatgt caataaaaaa ttatgctttt gaattcgtta atttcttaca
     1081 ttgatttttt cgcatcaaaa aacaaggcga aaagagccct ttgcggtaat ggctatatgc
     1141 cgtttttgct ttaaagtttt aattctttct aaagacgccg gcagagcata aaaaccccgc
     1201 cgctgattta cagcggggtt ataattagcc caaaacatca tagccctggt cttctatcgc
     1261 ggctttaatc gcgtccgcat tcagctttgc ggggtcgaac tcaacctcga cggttttgct
     1321 tttaaggtca acctcaactt tagaaactcc gtcaagagct tttaccgccc ttgtaattgc
     1381 cgctgagcag tgctcgcagg acataccctc taccttcatt attacgctgg tcatttttac
     1441 gcctccaagt tatatttttt actattttaa catagatttt taagcggaaa taagacttag
     1501 tcacctttta agccttattt ttccgtaaat attgacattt attttttcgg gcatataatt
     1561 atattaaaaa taaacattat gcttcggggc ggggtgaaag tccccaccgg cggtgaacgg
     1621 tcattgaacc gacaagtccg cgagcttagg ctgacagggt gagattccct gaccgacggt
     1681 atagtccgga tggaagaagt gcagtgtaat tgcggctccg tttttgcgga gcttttttgc
     1741 accccggcgc aatttcggag gtagcagaat gaaaaaacaa ctcaaaatgg tccagctctc
     1801 aatgctggca gcactcggcg tagtgcttat gcttatcgtc agattccctc ttttcccctc
     1861 agcgcccttc cttgagtacg acatgggcga tgtagcggta atcctaaccg ccttccttta
     1921 cagcccgtgg gcatctctct tagtgctggt gattgtgtcg ctgatacagg ctcttaccgt
     1981 cagcgcgtcc agcggatgga taggctttgt gatgcatgtt atttcaacgg gcgcgttcgt
     2041 tctggtatgc gggcttatct ataacagaaa gagaggcgca aaatcaaagg tttcaaagct
     2101 ctatttgccc gcagcccttg ccgcaggaac catagcaatg gtgcttgtga tgattccctt
     2161 gaacctgata tttacagtga gatttatgca ggtgccgagg gaagcggtac ttaatatgat
     2221 gatacccaca ataataccct ttaatgtact caagggcata attaactcgg ttgtggtatg
     2281 cgtactctac cctgcggtaa agaaaatact taaatcttca aagtcattga atcccgagtt
     2341 ataaaccttt acccctcctt ttcggagggg ttttttatct ctccattgag cagcagattt
     2401 ttatattgtt gtttttcata tactcggcgc cccagtcgta catggcgatg agtatcggct
     2461 caaggctcct gccaagctcg gttaaagagt attccacttt gggcggcact acggggtaaa
     2521 ccgttcggac aagcagcccg tcgttttcaa gctctctaag ctgctgggtc agcatttttg
     2581 gcgtcgcctc gggaatgagc ctttgaattt cgccgtatct catagttcca cgcatcaggt
     2641 gccataatat aagagatttg tattttccgc cgatcagctt taaagtcgcg ctgacgggac
     2701 agttaatctc ggttttctta gccatggaat cccctttact atttttttta tagtatagaa
     2761 caaatatgtg catacttgct gaaattacta tatgtgttat tataataaca taaggaaaat
     2821 ataccgtcaa ctctccgacg gcgaaaaagg agatgctgaa tttgacaaat aagactttta
     2881 atattaaggg catgacctgc gccgcttgcg ctcagaggat agagaaggtg gcgggcaggc
     2941 ttgacggcgt tgaaagcgcg gcggtgaacc tgaccgccga aaagctgttt gtaaagtacg
     3001 atgaggctaa agcgcgcccc gaggatataa tagccgccgt tacaaagata ggatacgagg
     3061 ccgaggagga gaaaacttca gccgttatca gccttcccat atcgggcatg acctgcgccg
     3121 cttgcgccca gaggattgaa aaggttttaa acaggcttga cggtgtctat aaggcaagcg
     3181 taaaccttgc cgcagagaag gcgacggtgg agtttgaccc gtccaaaatc agaacgggcg
     3241 caataaagga agctatagag aaactgggct ataaagtcct tgaggttaaa aaagaaagca
     3301 gggttgagga ggaaagggag agaaaaagaa aggaaataaa aaggctttgg actaagttca
     3361 ttgtggccgc cgtttttgct tttcctcttt tgtatatcgc catggggccg atgataagcc
     3421 ttcccgttcc ctccttttta gatcccgaca taagtccttt aaactatgct ttggttcagc
     3481 tcattctcgt aatccccgtc atggcggtgg gttacaggtt ctatacagtg ggctttaagg
     3541 ctcttataaa ccgctcgccc aatatggact cgctgattgc catcagcacc cttgccgcct
     3601 ttgtgttcag tgtttggaac acttatcaga ttataaaagg caatcatcac gccgcccatt
     3661 cgctgtactt tgagacggcg ggggttatca tt
//
LOCUS       DAONVQ010000151         3673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_17546_length_3673_cov_4.644279, whole genome shotgun sequence.
ACCESSION   DAONVQ010000151 DAONVQ010000000
VERSION     DAONVQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17546_length_3673_cov_4.644279"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLD98_08300"
     CDS             complement(<1..328)
                     /locus_tag="PLD98_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06281.1"
                     /translation="MNKENEKTLSRAGGKTGGSESHASPEKLQSSAKERHQNRKIAKS
                     RVVRTRSTDGSILIKAENAPDTYCDRFEEACLIRQTTHDILPPGTITPDELSKLDLEK
                     TKAFTAV"
     gene            complement(362..1114)
                     /gene="rlmB"
                     /locus_tag="PLD98_08305"
     CDS             complement(362..1114)
                     /gene="rlmB"
                     /locus_tag="PLD98_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HQA06282.1"
                     /translation="MNKEGQAKKPYLIIGKNAVKEALNSDRPVDYVLVAKGDKKNSLM
                     PLIAKCREKGIVVKEADVKKLDFACGHGNHQGIIMYAAAHEYCTVDDIFDKAEEMGEA
                     PFIVICDGIEDPHNLGAIIRTAEAAGVHGVIIPERRSVGLNFTVGKTAAGALEYMLVA
                     RVKNLNTTIEDIKSRGVWVWAADVDGIDYKEADFGGGVALIIGSEGNGISRLVKKNAD
                     GVVSVPMKGKINSLNASVAAAVLMFEISSRRY"
     gene            complement(1324..3084)
                     /locus_tag="PLD98_08310"
     CDS             complement(1324..3084)
                     /locus_tag="PLD98_08310"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HQA06283.1"
                     /translation="MRLLKNELESILVGSRVEKIHQPTKNEFVFHFRSRTGAYKLLLS
                     ASGSTPRVNITEHPPENPANPPMLCMLFRKHLSGAILKGIEQSGLERALLFRFDAVNE
                     IGNRVLRTLAVEIMAAYSNIILIDEENRIIDSIKRVDSLKSSVREILPGREYISPPPQ
                     DKLSLLEISADEAAEAVFKNLPGTALSSAILKTIGGLSPLVCRELAFLATGSDPNLSE
                     LNENTKATLAFFLDQLKLRIEKNMAEPYIYYDNHDSPVDFCFMPISQYGNMAKGVKLN
                     SLSQVLDEFYYEKDRILRINSRASDLIKLINKLSERTARKLEAQREELKQSEDREIKK
                     IYAELINANLYRLKKGESFYVLENFYDEGKEIKIPAKPELTPAENARLYYKEFRKAQT
                     AEKILREQIEKGFEEINYFETVLDALSRAPSDSEIDDIRRELAEGGYIKERRSRKDLD
                     KKRAALPFIELKSPDGFKVLVGRNNTQNDRLSFRKAAKDDIWFHAQKVPGSHVILVTE
                     GKEPTKEAMEFAAACAAAFSKARNATTVPVDYTEVKNLKKPPGAKPGYVIYHVYSTMM
                     IKPLSDKEIEAAKAGEGKDF"
     gene            complement(3105..>3673)
                     /locus_tag="PLD98_08315"
     CDS             complement(3105..>3673)
                     /locus_tag="PLD98_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQA06284.1"
                     /translation="HPPIFEPTKTFTADMLAFKAAAQGVSVIAAHTNFDGASDGVSDT
                     LAKTLGLKRIKAVNVPGETSSILREGRTSFETADEFARYIAKKLGGGVRFCDGGKKIK
                     KVAVSGGSGKSFVDFVISAGYDAFVTGEVSYNNFLDAKEKGLSIFAAGHFETEFPAMN
                     ILTEQLKIACPTLKIVLCGQQSPIMTVV"
BASE COUNT          815 a          951 c          836 g         1071 t
ORIGIN      
        1 tcactgcggt aaaagccttt gtcttttcca aatcgagttt actcagttca tcgggtgtaa
       61 tggtgccggg tgggagaata tcatgagttg tttgtcttat aaggcaggcc tcttcaaacc
      121 tgtcgcaata tgtatcggga gcgttttccg cttttataag tattgagccg tccgttgaac
      181 gggtccttac gaccctgctt ttcgctatct ttctgttctg gtgccgctct ttcgcagagg
      241 actgcagctt ttcgggagag gcatggcttt ccgagccgcc cgttttgccg ccggctctgc
      301 tcaatgtttt ttcgttttcc ttattcaagc ctttccctcc ttttcagaat ttgctgcatg
      361 tttaatatct gcggctcgaa atttcaaaca tcagcaccgc cgccgccacg gaggcgttga
      421 gagagttaat cttgcccttc atgggaacgg aaaccacgcc gtctgcgttc ttttttacaa
      481 ggcgggatat gccgttgccc tcagagccga taataagagc cacgccgccg ccgaaatcgg
      541 cttccttata atctattccg tcaacatccg cggcccatac ccacacgccg cggcttttaa
      601 tatcctcaat ggtagtattc agattcttaa cccttgccac gagcatatat tcgagagcgc
      661 ccgcagccgt cttgccgacg gtgaaattaa gccccacgct tctgcgttcg gggataatca
      721 cgccgtgaac ccccgcagcc tcagcggttc tgataattgc gccaagatta tgcgggtcct
      781 caatgccgtc gcagattact ataaagggag cttcgcccat ttcctccgcc ttgtcgaaaa
      841 tatcatcgac tgtgcagtac tcatgggccg cggcgtacat gattatgccc tgatggttgc
      901 cgtgaccgca ggcaaaatcc agttttttaa catccgcttc tttgaccaca atgccctttt
      961 ccctgcattt tgcaatcagg ggcataaggg agttcttttt gtctcccttc gccacaagca
     1021 cataatcaac gggtctgtcg gaattcagcg cctctttaac ggcgttcttt ccgattatga
     1081 ggtatggttt ttttgcctgc ccttctttgt tcatactgcc acctttttaa gttcctgcga
     1141 gcttttttcc gctcacgaaa aaagcccgaa ttatattaaa atattctacc acaccaatga
     1201 caaaaaacaa tagggtgcgg caattttttg tataaagtgt caaaaattca ataaatatta
     1261 tatcaattgc cgacggattt agtaaagaaa atagaaatgt atcttttcgg taaaatgcgt
     1321 cactcaaaaa tctttgcctt cgcccgcctt tgcggcttct atctccttgt cgctcagcgg
     1381 ttttatcatc atagtgctgt aaacatggta gatgacatag ccgggctttg cgccgggggg
     1441 ctttttaagg tttttaacct ctgtgtagtc aaccggaacg gttgtggcgt ttctcgcctt
     1501 gctgaacgcc gcggcgcacg cagccgcaaa ctccatagcc tctttagtcg gctctttgcc
     1561 ctcggtcaca agtattacat gtgagccggg caccttttgg gcgtggaacc aaatatcatc
     1621 tttagctgcc tttctgaatg agagcctgtc gttctgggtg ttgttcctgc ccacaagtac
     1681 cttaaagccg tcgggggatt taagctcaat gaaaggcaga gcggcgcgct ttttgtcaag
     1741 gtcttttctt gagcgccttt ctttaatgta gccgccctcc gccagttccc ttcgtatatc
     1801 gtcaatttcg ctgtcggagg gagcccttga aagagcgtcg agaacggtct caaaataatt
     1861 aatttcctca aagccctttt caatctgctc ccttaatatc ttctccgcag tctgcgcctt
     1921 tctgaactcc ttgtaataga gccttgcgtt ttccgcggga gtaagctcgg gctttgctgg
     1981 gatttttatt tctttgccct cgtcataaaa gttttccagc acataaaagc tttcgccctt
     2041 tttgagcctg tagagattag cgtttataag ctcggcgtag atctttttta tctccctgtc
     2101 ctcggactgc ttcagttcct ccctttgagc ctccagcttc cttgcggttc tctcggaaag
     2161 cttgtttata agctttatca aatccgaggc gcgggagtta atccttaaaa tcctgtcctt
     2221 ttcgtagtaa aactcgtcca gaacctgaga gagagaattt agctttactc cctttgccat
     2281 attgccgtac tgagaaatgg gcataaagca aaaatcaacg ggggagtcgt ggttgtcgta
     2341 atatatgtat ggctcagcca tatttttttc aattctgagc ttgagctggt cgaggaaaaa
     2401 cgccagcgtc gctttggtgt tctcatttaa ttctgaaaga ttcgggtccg agcccgtggc
     2461 aaggaaagcc aactccctgc acacaagggg agagagacca ccgattgttt taagtatagc
     2521 cgaggaaaga gcggtgccgg ggaggttttt aaataccgcc tcagcagcct catcagcgga
     2581 aatttcgaga agactcagtt tatcctgcgg gggaggggag atatactccc tgccgggcag
     2641 aatttctctt accgaggatt taagactgtc gaccctctta attgaatcga ttatcctgtt
     2701 ctcctcgtca atcagaataa tattgctgta tgcggccatt atctcaaccg ccagagtcct
     2761 taaaaccctg ttgccgattt catttaccgc gtcgaacctg aaaagcaaag ccctttcaag
     2821 cccgctttgc tctatgcctt tcagtatcgc ccccgaaagg tgctttctga aaagcatgca
     2881 cagcatgggg ggatttgcgg ggttttcggg cgggtgctcg gttatgttta ccctcggagt
     2941 gctgcccgac gcggacaaaa ggagcttata cgcccccgtt cgcgaacgaa agtgaaaaac
     3001 aaattcattt ttagtcggct gatggatttt ttctaccctc gagccgacta aaatgctttc
     3061 cagttcattt tttaaaagtc tcaaatatat tccgtcaagc gccattatac caccgtcatt
     3121 atgggacttt gctgcccgca gagcacgatt ttcagcgtgg ggcaggctat tttaagctgc
     3181 tctgtcaaaa tattcattgc ggggaattcg gtttcaaaat gccctgccgc gaaaatggaa
     3241 agccccttct cctttgcgtc caggaaatta ttgtaggaga cctctccagt aacaaaagcg
     3301 tcatagccgg ctgagattac aaaatccaca aaggacttgc ccgagccgcc gcttacggcg
     3361 accttcttta tcttctttcc gccgtcgcag aacctgacgc cgccgcccag cttctttgcg
     3421 atgtaccttg cgaattcgtc agcggtttca aagctggttc tgccctctct taaaatagag
     3481 gaggtctctc cgggcacatt tacggcttta atccgcttta agcccagcgt ctttgccaga
     3541 gtgtcgctga caccgtccga cgcgccgtca aaatttgtat gcgccgcgat tacgctgacg
     3601 ccttgagccg ccgctttgaa tgccagcata tctgctgtaa aggtctttgt cggctcaaat
     3661 atgggcggat gat
//
LOCUS       DAONVQ010000152         3592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_18016_length_3592_cov_3.394119, whole genome shotgun sequence.
ACCESSION   DAONVQ010000152 DAONVQ010000000
VERSION     DAONVQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18016_length_3592_cov_3.394119"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2325
                     /locus_tag="PLD98_08320"
     CDS             <1..2325
                     /locus_tag="PLD98_08320"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQA06285.1"
                     /translation="EGLSKEDVGREGFLERAWKWKEQYGGIIIEQLKKLGSSCDWSRE
                     RFTLDEGCNRAVNTVFVNLYKEGLIYRGERMINWCPDCLTSISDAEVEHEEKGGHFWH
                     IKYPVKDSDRYIVIATTRPETMLGDTAVAVHPDDERYKDLIGKTLILPLVGREIPIIA
                     DTYVDMEFGTGAVKITPAHDPNDFEVGLRHNLPVIDVMTDDGKMSELAGEKYAGLDRF
                     ECRKLVVKDLEEIGALLKTEDITHSVGTCYRCSTVVEPRISMQWFVKMEPLAKPAIEA
                     VKKGDIRFVPERFEKIYFHWMENIKDWCISRQLWWGHRIPAWYCDDCGHITVAVETPD
                     KCEKCGSAKIHQDEDTLDTWFSSALWPFSTLGWPDKTFDYEHFYPTNTLVTAYDIIFF
                     WVARMIFSGLHHTGKAPFNTVFIHGLVRDELGRKMSKSLGNGVDPLKIIEDYGADALR
                     FSLATGNSPGNDMRFSEKKVEASRNFSNKLWNAARFVLMNLSDDEPAPHIPDELNLED
                     KWIISRYNTVLKEVTESLEKFEIGIAVQKLYDFIWDEFCDWYIELSKIRLQKEGKEKE
                     NAKAVLVFVLSETLKLLHPFMPFITEEIWQTLPHEGETIMLSPWRKFDESLSFPEAET
                     EIQRIMEAIRAIRNRRAEMNVHPSKKANLFIKTAYKDTFSYADEFFKKLAGASEVTLV
                     DDYDIEGAVSVVTTDAAIYIPMSELVDFEAERKRLEKELKATEEKLAQIEAKLSNEGF
                     LAKAPEHVINAQKEAAAGLKEKIAMLKEDLSKLG"
     gene            2399..>3592
                     /locus_tag="PLD98_08325"
     CDS             2399..>3592
                     /locus_tag="PLD98_08325"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HQA06286.1"
                     /translation="MEPFEPYEFFESLLQRGINPGLEGIKALLKELGNPQDRLCFIHI
                     AGTNGKGSTAAYISGILTEAGIKNGLFTSPFVTDRRESIQLDSAYIPEPEFKRVGLEI
                     KKAADKLSADGIEVTEFEAMTAAAIKFFADSGCAVAVLEAGMGGRLDATNIIENTAAA
                     VLTSISLDHTDFLGDTVEKIAGEKAGIIKKGCAVVTSASQSEEAYAVIKEKAEKEGAE
                     LYTVKPESEDLKFDRVCFSKTNESESEIKLAQAISVSNKYQKENAMLAVMAVKALEGR
                     IGKVSDGAIKAGIEKTFCPARLEVISKKPFILLDGGHNEAAITALKDYLESKLGGKRL
                     LGLVSMMKDKDYKRAAELIGNKFVKIIAAQCANPRAVDSSVLADVFSPYCKTKAVPSP
                     DDALKT"
BASE COUNT          998 a          837 c          950 g          807 t
ORIGIN      
        1 gagggtcttt ccaaagagga tgttggcaga gaaggtttcc tcgagagagc gtggaaatgg
       61 aaggagcagt acggcggcat aattattgag cagcttaaaa aactcggctc ctcctgcgac
      121 tggagcaggg agcgctttac ccttgacgag ggctgcaacc gcgccgtcaa caccgtattt
      181 gtaaaccttt ataaagaagg tcttatttac aggggcgaaa gaatgattaa ctggtgcccc
      241 gactgcctga cttcaatctc cgacgcggag gttgagcacg aggaaaaggg cggtcacttc
      301 tggcacatca aataccccgt aaaagacagc gacagatata tcgtcatcgc cacaacccgc
      361 cccgaaacaa tgctgggcga taccgccgtc gcagttcatc ccgatgatga gagatacaag
      421 gatttaatcg gcaagactct tattctcccc cttgtcggca gggaaattcc cattattgcg
      481 gacacctatg ttgacatgga gttcggtacg ggcgcagtta agattactcc cgcgcacgac
      541 ccgaacgact ttgaggtagg tctcaggcac aacctgcccg tcatagatgt tatgaccgat
      601 gacggcaaga tgagcgagct cgccggcgaa aagtacgcgg gtcttgacag attcgagtgc
      661 agaaagcttg ttgttaagga ccttgaagaa atcggcgcgc tcttaaaaac tgaggatata
      721 acacacagcg tgggaacatg ctaccgctgc agcacggttg ttgagccgag gatttcaatg
      781 cagtggttcg ttaagatgga gccccttgca aagcccgcaa tcgaggctgt taagaagggc
      841 gacatccgct ttgttcccga gcgttttgaa aagatatact tccactggat ggagaatata
      901 aaagactggt gcatttcccg tcagctctgg tggggtcaca ggattcccgc ttggtactgc
      961 gacgattgcg gtcacatcac cgtagcggtt gagacacccg acaagtgcga gaagtgcggc
     1021 agcgcaaaga ttcatcagga cgaggatacc cttgacacct ggttctcatc cgctctctgg
     1081 cccttctcca cgctgggctg gcccgataag acatttgact atgagcattt ctatcccaca
     1141 aatacccttg taaccgcata tgacattatc ttcttctggg ttgccaggat gatattctcg
     1201 ggactccacc atacaggcaa ggctcccttc aacacagtat tcattcacgg ccttgtaagg
     1261 gatgagctgg gcagaaagat gtccaagtcc ctgggcaacg gcgttgaccc gctaaagatt
     1321 atcgaggact acggcgcaga cgctctgaga ttctcccttg ccacaggcaa cagccccgga
     1381 aacgacatga gattctccga aaagaaggtt gaggccagca ggaacttctc caacaagctc
     1441 tggaacgcgg caaggttcgt tcttatgaat ctatccgacg acgagcccgc tccccacatc
     1501 cccgatgagc tgaatctcga ggacaagtgg ataatcagcc gctacaacac cgttctcaaa
     1561 gaggttaccg aatcccttga gaagtttgag ataggcattg cggttcaaaa gctctatgac
     1621 tttatctggg acgagttctg cgactggtat attgagcttt caaagataag gctccagaaa
     1681 gagggcaagg aaaaggagaa cgcaaaggct gttcttgtgt tcgttctgag cgaaacgctg
     1741 aaactcctgc accccttcat gcccttcatt accgaggaaa tctggcagac cctgccccat
     1801 gagggcgaga caataatgct ctcaccctgg aggaagtttg acgagtccct ttcattcccc
     1861 gaggcagaga cggaaatcca gagaatcatg gaggctataa gggctatcag aaaccgcagg
     1921 gctgagatga atgttcaccc gtcaaagaag gcaaatctct ttatcaagac cgcatataag
     1981 gataccttca gctatgcgga tgagttcttt aaaaagctgg cgggcgcgtc agaggtcact
     2041 ttggttgacg actacgacat agagggcgcc gtttctgttg ttaccaccga cgccgctatc
     2101 tatatcccga tgagcgagct tgtggacttt gaggcagagc gcaagaggct tgaaaaagag
     2161 ctgaaagcaa ctgaggaaaa gctggctcag atagaggcaa agctgtctaa cgagggcttc
     2221 ctcgcaaagg ctcccgagca tgtaattaac gctcagaagg aggcagcggc gggtcttaaa
     2281 gaaaaaatcg ccatgcttaa agaggacctt tcaaagctgg gctaaataat caaaatgaat
     2341 tataatagag gaagtggctt atgccgtttc ctctttttta tagtcagagg tgctgagatt
     2401 ggaaccgttc gaaccctatg agttttttga atccctttta caaagaggga taaacccagg
     2461 tcttgagggc ataaaggctc ttttaaagga gcttggaaat cctcaggaca ggctttgttt
     2521 tatccatatt gcgggtacta acggcaaggg cagcaccgcc gcatatatct ctgggatttt
     2581 aaccgaggcg ggaatcaaaa acggactttt tacctcgccc tttgtaacgg acagacggga
     2641 gagcatacag cttgattccg cttatattcc cgagccggag tttaaaaggg tcggattaga
     2701 aataaaaaag gctgcggaca agctctcggc ggacggcatt gaagttaccg agtttgaggc
     2761 tatgaccgcg gcggcgatta agttctttgc cgattcggga tgcgctgtcg ccgtgcttga
     2821 ggccggaatg ggcggcaggc tcgacgccac aaatataata gaaaacactg ctgctgcggt
     2881 gctgacaagc atatcccttg accatacgga ctttctgggc gatactgtcg aaaaaatagc
     2941 cggcgaaaag gcgggcataa ttaaaaaagg ctgcgcggtg gtaacctcag cttctcaaag
     3001 tgaagaagcg tacgccgtta taaaggaaaa ggcggaaaaa gaaggagcgg agctttatac
     3061 cgtaaagccc gaatctgagg atttgaaatt tgacagagtt tgcttttcaa agacaaatga
     3121 aagcgagtcg gaaataaaac ttgcccaagc aatctccgtt tcgaataaat atcaaaaaga
     3181 aaacgctatg ctggctgtaa tggctgtaaa ggcacttgag ggaaggatag gaaaagtatc
     3241 cgacggcgcg ataaaagcgg gaattgaaaa aaccttttgc cccgcaaggc ttgaggtgat
     3301 ttctaaaaag ccctttatcc tgcttgacgg cgggcataat gaagcggcga ttacggcttt
     3361 gaaagattat ctggagagca agctcggcgg aaagcggctt ttgggtcttg tgtctatgat
     3421 gaaagataag gactacaaaa gagcggcaga gcttattggc aataaatttg taaagattat
     3481 tgccgcacaa tgcgcaaatc ccagggcggt tgacagctct gttttggctg atgtcttttc
     3541 gccatattgc aaaacgaagg ctgtcccctc ccccgacgac gctcttaaaa cc
//
LOCUS       DAONVQ010000153         3555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_18247_length_3555_cov_3.542000, whole genome shotgun sequence.
ACCESSION   DAONVQ010000153 DAONVQ010000000
VERSION     DAONVQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18247_length_3555_cov_3.542000"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PLD98_08330"
     CDS             complement(<1..708)
                     /locus_tag="PLD98_08330"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HQA06287.1"
                     /translation="MSTRNHEITTRQNVLNPDGSLREPGYSKRPIQIYSRDQIKASKF
                     RIKEWDYYLITSNEYGLALTMADLGYVGMLSASLLDFRIPWEHTETALVPFPMGRFNL
                     PASSTEGHSTFSNKRVTMEFFNNKGSRDILCTFKNFLDGKTLSCEIHLDEPEMDSMVI
                     SIPWKEDKKAFYYNRKINCMRADGQVKFDGKVYKFDPCTDFGCLDWGRGVWTYKNRWL
                     WATGSGLATDGVPFGFNL"
     gene            complement(780..1769)
                     /locus_tag="PLD98_08335"
     CDS             complement(780..1769)
                     /locus_tag="PLD98_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQA06288.1"
                     /translation="MRFKEIDKSGTVEGFALVKHMEKKTSKNGSYYLDMVLADLDGEI
                     SAKLWDYKEDIFPEIPLHSIVKVRGVILQYNGMPQFRIDRIRPAIAADNVKLEDFVPT
                     AEYTGEMMMAEIRRVVEGFKDSQLKALVNALLDEYEDRLLVWPAAVKLHHAFRGGLLY
                     HTLSIIRLAQAVSKIYPSVDEDLLLTGVILHDIAKTEEFNLSPAGLAENYTADGMLIG
                     HLVRGAMAVERVGKELKIDHETLMCVQHMLISHHGSPEYGAVVRPMFLEAELLSQLDL
                     LDSRIYEIVSATAEVQKGDFTPRQWALEDRRLYNHGRKPLIPEAQLGINPEKN"
     gene            complement(1791..2339)
                     /locus_tag="PLD98_08340"
     CDS             complement(1791..2339)
                     /locus_tag="PLD98_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQA06289.1"
                     /translation="MKKLYNWAVKLPIIRNIMQKELFQKLFSYEVFIYLFFGVLTTAV
                     NLVSFKLFNMLLGEGTLFVIKVASKQLPVGSYLVANAIAWVIAVLFAFFTNKLYVFES
                     KSFKFKVAFAEFMAFMGARVFSLIVEQFGLVFFVELLKLREMTAKIVLAVIVVILNYF
                     FSKFFIFKERKAKNLDLAEGGK"
     gene            complement(2353..3141)
                     /locus_tag="PLD98_08345"
     CDS             complement(2353..3141)
                     /locus_tag="PLD98_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HQA06290.1"
                     /translation="MSVKLGMCTSAKDLERIKKLAEIGYDYVEINFTGMTEMPEDEFE
                     AFLKVLEETNFKCEAANCFIPGEIKLTGENVDYDKVREFVSKGMARAERAGIKSVVFG
                     SGGARRVPEGFPMDKAYEQLVKFLKEYAGPIAARHGVAIAIEPLRSQETNIINKVSEG
                     VELARKVGLPNVKGLGDIYHMATEYDSDDELAKFRGEIQHTHISHPLTRYAPKKDDGY
                     DYSVFLKAVIDSGCERCSVEGRLDNFPEDAVEALEVLKNAVKNI"
     gene            complement(3180..>3555)
                     /locus_tag="PLD98_08350"
     CDS             complement(3180..>3555)
                     /locus_tag="PLD98_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQA06291.1"
                     /translation="IMLAIKCVLSDADTIGTLIFDEIDTGVSGRAAGKVAIKLKETAK
                     SRQVICVTHLSQLACMADSHLLIEKTVSGGSTFTSVRELDFEGRKREIARINGGMNIT
                     PALLKSAEEMLIAAGIAPTHGS"
BASE COUNT          802 a          885 c          861 g         1007 t
ORIGIN      
        1 aaggttaaag ccgaagggta cgccgtcggt cgcaagtccg ctgcccgtag cccaaagcca
       61 gcggtttttg tatgtccaaa cgcctctgcc ccagtcaagg cagccaaagt cggtgcaggg
      121 gtcgaactta taaaccttgc cgtcgaattt gacctgaccg tcagccctca tgcagttgat
      181 tttgcggttg tagtagaagg cttttttgtc ctctttccac gggatggaga ttaccattga
      241 atccatttcc ggctcgtcaa ggtggatttc gcaggaaagg gtcttgccgt caaggaagtt
      301 cttgaaggtg cagagtatgt ccctgctgcc cttgttgttg aagaactcca tggtgactcg
      361 tttgtttgag aaggtgctgt gaccctcggt actggatgcc ggcagattga acctgcccat
      421 cgggaacgga accagagcgg tttcggtatg ctcccacggg attctgaagt ccaaaagcga
      481 cgcggacagc atgcctacat agcctaagtc agccatggta agggcgagac cgtactcgtt
      541 ggatgtaatg aggtagtagt cccattcctt gattctgaac ttggaggctt tgatctggtc
      601 cctgctgtaa atctgaatcg gacgctttga atagccgggc tccctcagcg agccgtcggg
      661 attaagtaca ttctgtctgg tggtgatttc gtgatttctt gtagacatat atatatcctc
      721 ccgtaaatta ttattggcaa tttggctgtg cgatggggtc ttatggcttt tcttcaggat
      781 taattctttt cagggttaat tccaagctgt gcttcgggta taaggggctt tctgccgtgg
      841 ttgtaaaggc gtctgtcttc cagcgcccac tgacggggcg taaagtcgcc cttttgaacc
      901 tcggctgttg cggaaacgat ttcgtatatc cttgaatcga gaaggtcgag ctgggataaa
      961 agctctgcct caaggaacat gggtctgaca acagcgccgt actctggcga gccgtggtgg
     1021 gagatgagca tgtgctggac gcacataagg gtttcgtggt cgattttgag ttccttgccc
     1081 actctttcca ccgccatggc accccttaca aggtgaccta tcagcattcc gtcggctgtg
     1141 tagttttcgg caagtcccgc gggggagaga ttaaattcct cggtctttgc tatgtcatgg
     1201 agaatgacgc ccgttaaaag caaatcctca tccacgctgg ggtaaatttt gcttaccgcc
     1261 tgagcaagac gaatgattga aagggtatga tacaaaagtc cgcctctgaa cgcgtggtgg
     1321 agttttaccg ccgctggcca gacaagaagt cggtcctcgt attcatccag cagggcgttt
     1381 acaagagctt taagctgact gtccttaaaa ccctcaacga cgcgccttat ctccgccatc
     1441 atcatttcgc ccgtgtattc ggcggtgggc acaaagtcct caagtttaac attgtccgct
     1501 gcaatcgcgg gtcttatcct gtcaattctg aactggggca tgccgttgta ctggagaatt
     1561 actcccctta ccttgacaat gctgtgcagc gggatttcgg ggaagatatc ctccttgtaa
     1621 tcccacagct ttgcggatat ttctccgtcc aaatcggcga gcaccatatc aaggtaatac
     1681 gagccgtttt tggaggtctt tttctccatg tgttttacaa gggcaaagcc ctccacagtg
     1741 ccgcttttgt caatctcttt gaatctcatt gcgctttcct ttcaaggatt tcattttccg
     1801 ccttcggcta aatccaagtt tttagccttt ctctctttga atataaagaa cttactgaag
     1861 aagtagttga gaatcaccac tataaccgca agcactattt tcgcggtcat ttctctgagc
     1921 tttaatagct ctacaaagaa cacaagcccg aattgctcaa caataaggga gaatactctt
     1981 gcgcccatga atgccatgaa ctcggcaaag gctaccttga acttaaagct tttgctttca
     2041 aaaacataga gcttgttggt aaagaacgcg aacaaaacgg ctataaccca ggcgatggcg
     2101 tttgccacaa ggtatgagcc aacggggagc tgctttgacg cgacttttat cacaaacaat
     2161 gtgccttcgc ccaaaagcat attaaaaagc ttaaagctta caagatttac tgcggtggtt
     2221 aaaactccga aaaagagata tatgaatacc tcataggaga agagcttttg aaacagctct
     2281 ttttgcatga tgtttcggat aatgggcaat ttcaccgccc agttataaag ttttttcatt
     2341 ctctgctccg cattatatgt tttttaccgc gtttttaaga acctcaaggg cttcgacagc
     2401 gtcctcgggg aagttgtcaa gtcttccctc aacggagcat ctttcgcagc cgctgtcgat
     2461 gacggccttt aaaaataccg aatagtcgta gccgtcgtcc tttttggggg cgtatctggt
     2521 aagggggtgg gagatgtggg tgtgctgaat ctcgccccta aattttgcca gctcgtcgtc
     2581 gctgtcatat tcggttgcca tgtgatatat gtcgccaagg ccctttacat tgggaaggcc
     2641 cacttttctt gcaagctcaa cgccctcgga aaccttgttt atgatgttgg tttcctggct
     2701 tctcagcggc tctattgcga tggcgactcc gtgccgggcg gctatggggc ccgcgtattc
     2761 tttaaggaat tttacaagct gctcataagc cttatccatg gggaaacctt cgggcactct
     2821 gcgggcgccg ccgctgccga aaacaacgga cttaatgcct gctctttcag cccttgccat
     2881 acccttgctg acgaattccc tgaccttatc atagtcaaca ttttcgcccg taagtttaat
     2941 ctcaccgggg ataaagcagt tcgcggcttc gcatttaaag tttgtttcct caagcacctt
     3001 taaaaatgcc tcaaattcat cctcgggcat ttcggtcatg cctgtgaaat ttatctccac
     3061 atagtcatag ccgatttccg caagtttctt gattcgctcc aaatccttgg cggaggtgca
     3121 cattcctaat tttacactca taatatgaac tcctcttatg aaaaattata aactttgtat
     3181 taacttccat gtgtcggggc gattcccgcc gctatcagca tctcctctgc gctctttaaa
     3241 agggcggggg tgatgttcat gccgccgttg attctggcga tttccctctt tctgccctca
     3301 aaatcaagct cccgcaccga tgtaaaagtg gagccgccgc tgacggtctt ttctatgagc
     3361 aggtggctgt ccgccatgca ggcaagctgg cttaaatggg tgacgcatat gacctgacgg
     3421 ctctttgcgg tttctttgag ctttatggcg accttgcccg ccgctctgcc gctgacaccc
     3481 gtgtcgattt cgtcaaatat cagcgttccg atggtgtccg cgtcggaaag aacgcacttt
     3541 atcgccagca ttatg
//
LOCUS       DAONVQ010000154         3525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_18429_length_3525_cov_4.673487, whole genome shotgun sequence.
ACCESSION   DAONVQ010000154 DAONVQ010000000
VERSION     DAONVQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18429_length_3525_cov_4.673487"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="PLD98_08355"
     CDS             complement(<1..1447)
                     /locus_tag="PLD98_08355"
                     /inference="COORDINATES: protein motif:HMM:NF019161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HQA06292.1"
                     /translation="MKKLTHKIISLCLIFSVLMLSVSAFSAFAAESQLYVIKDTTGVY
                     TKPALPPVGEKLTDIHKYAIVTVVEVTNGWGKIYIQGTEGYIHLSMTEPYSGDPADAS
                     SIESINIESLPYKTVYTDGESFSAEGIAVSATLKSGEKVALKGFDIIVPPMTLTRSDL
                     EDKQQTVTVKYGPGTALTAGFLITVKRLPIDSLKVVSWPKLEYKEGERIDLTGLKVMA
                     IYTDGSDKTDVTNELIEKTTGKSISELQGKILEPGKYKFEYVYKYNDKKITLRIKVNA
                     KKLIGIRVSSMPTYNVYINKNTLDLRGFVLAAVYDNGTSEPIPQGAYTVSHGELKPNQ
                     DNLITVSYGGFAVEFTIFLEEKKVTELVVKQPPDRTMFQLGEQLDTTGLVITLKYNDG
                     TQRDITEGFTVIAPDMSLIGKKLVKVQAHGLEVTFQIYITDRLYTKGDVDNNGKINAA
                     DARLVLRYYAKLENLGAIQLMAADVDGNGKVN"
     gene            1916..3001
                     /locus_tag="PLD98_08360"
     CDS             1916..3001
                     /locus_tag="PLD98_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQA06293.1"
                     /translation="MGKKFRVGIIGCGGIANGKHMPALKNTGRVDMVAFCDIIEERAV
                     KAAREYGTPDAKVYTDYKELLKDDSIEIVHVCTPNRSHSFITIDALEAGKHVMCEKPM
                     AKTYEEAKAMVEAAKRTGKKLTIGYQNRQKPQSLYVKECCDNGELGEIYYARAIALRR
                     RAVPTWGVFLNEYEQGGGPLIDIATHALDMTLWMMNNYEPKMVVGNIFKKLGKQRNTA
                     NAWGDWDPEQFTVEDSAFGYVVMKNGAVISVESSWALNIADPDEANVLLCGDKAGADT
                     LHDKVRINYVKNNRQVIECPDFSAGGVAFYDGKKVSPKDLEAQKWLDAIEFDTDPCVT
                     PEQALVVTQILEAIYTSAKTGQPVFFE"
     gene            3082..>3525
                     /locus_tag="PLD98_08365"
     CDS             3082..>3525
                     /locus_tag="PLD98_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HQA06294.1"
                     /translation="MAMTLGIISSWSEEGFKYAADLGLKAVEFCYNVGLDAAELKKLV
                     PDLKKWSEKYDVKVLSIGRWGAPKFDAETGELIEEELNHNLTLLDVCAELGCPVFNTG
                     VNYVESKTYSENLENAISFLKKLVDYGKERNVKIAVYNCDWANFVR"
BASE COUNT          908 a          829 c          857 g          931 t
ORIGIN      
        1 cgttgacctt gccgttgccg tccacatcag ccgccatgag ctgaatggcg cccagattct
       61 cgagcttcgc gtagtacctt agtaccaatc ttgcgtcggc ggcgttgatc ttgccgttgt
      121 tgtccacatc gcccttggtg tagagcctgt cggttatata tatctggaat gttacttcaa
      181 gaccgtgcgc ctgaactttt acaagcttct tgccaatcag gctcatatcg ggcgctatga
      241 cggtaaagcc ttcggttatg tccctttgag tgccgtcgtt gtattttaga gtaattacaa
      301 gaccagtggt gtcaagctgc tctcccaatt ggaacattgt cctgtcgggc ggctgcttta
      361 caacaagttc ggtaaccttt ttttcttcaa ggaagattgt aaactctacg gcaaagccgc
      421 cgtatgaaac ggttatcaga ttatcctgat tgggcttcag ctccccgtgg cttacggtat
      481 acgcgccctg aggaatcggc tcgcttgtgc cgttgtcata gacggcggca agcacaaagc
      541 ctcttaaatc aagggtgttc ttatttatat agacattgta ggtgggcatg gaagaaactc
      601 tgatgccgat gagctttttg gcgtttacct taatgcgcag ggtgattttc ttgtcgttgt
      661 acttatagac atactcgaat ttatatttac ccggctctaa gattttgccc tgaagctcag
      721 atatgctctt gcccgttgtc ttttctataa gttcgttcgt aacatcggtt ttatcgctgc
      781 cgtccgtata gattgccatg actttaagac ccgtaaggtc gattctctcg ccctctttgt
      841 actcgagttt gggccagctc acaactttca atgagtcaat gggcagcctt tttaccgtaa
      901 tcaaaaagcc tgcggtgagc gcggtgccgg ggccgtattt tacggtaacg gtctgctgct
      961 tgtcctcaag atcggagcgg gtcaaagtca tgggcggcac tataatatca aagcctttca
     1021 aagctacctt ttcgccgctt ttaagggttg cggaaacggc aatgccctcg gcggaaaagc
     1081 tctccccgtc ggtatagacg gttttgtagg gaaggctttc gatattgatt gactcgattg
     1141 acgatgcgtc ggcggggtcg ccgctgtaag gctcggtcat ggaaagatgt atgtatccct
     1201 ctgtgccctg aatatatatt ttgccccagc cgttggtgac ttcaacgacg gtaacgattg
     1261 cgtatttgtg tatgtcggtc agcttttcgc cgacgggcgg cagggcgggc tttgtatata
     1321 ctcccgtggt gtcttttatt acatagagct gactctcagc ggcaaaagcc gaaaaagccg
     1381 atacggagag catcagcacg gaaaaaatga ggcaaaggga tattatctta tgggtgagct
     1441 ttttcatttt ctcatccttt catattgaca aaccgtaaag catagagttt agcggacaaa
     1501 tccgcagctt gtataaaaac ttcctgtcat aaatcagact gcgggaaatg gcagattgtt
     1561 ctcgcaaatc ctcaagttaa ttatagtgcc gggcggcttt atgtgtcaat tgaaacaaat
     1621 ttacagattt gtttgcccgg aattgaataa aaatggaaaa tccttgattt gacgcggttt
     1681 ttgggtcttt tatgcgccag aaaacaatag gcaaaatgta taaaaatcta ctattatttt
     1741 ttaccttgcg ctttttactc ttaaccttga taattaagca gattaactta taattagtca
     1801 taagggctat tgtcaaggct ctctgctttg ctgccctgtt tttgcttttg actcttttag
     1861 agaggcttct ctcttggaat aaaaaatcaa actttcagtt aatggaggat attttatggg
     1921 taaaaaattc agagtcggca tcataggctg cggcggtatt gcaaacggca agcatatgcc
     1981 cgcgctcaaa aacacaggca gagttgacat ggttgcattc tgcgacatca tcgaagaaag
     2041 agcagtcaaa gctgcccggg agtacggcac tcccgacgct aaggtctaca ccgactacaa
     2101 agagcttctg aaagacgact ccatcgaaat cgttcatgta tgcacaccca accgttcaca
     2161 ctcctttatc accatcgacg cccttgaggc aggcaagcat gtaatgtgcg agaagcccat
     2221 ggcaaagacc tatgaagagg caaaggcaat ggtcgaggcc gcaaagcgca caggcaaaaa
     2281 gctcaccata ggctatcaga accgccagaa accccagagc ctttatgtaa aggaatgctg
     2341 cgacaacggc gagctcggcg aaatctatta cgcaagagcc atcgctcttc gccgcagagc
     2401 ggttcccacc tggggcgtat tccttaacga gtatgagcag ggcggcggcc ccctcatcga
     2461 catcgcaacc cacgctcttg acatgaccct ttggatgatg aacaactacg agcccaagat
     2521 ggtagtaggc aacatcttca aaaagcttgg caagcagaga aacacagcca acgcttgggg
     2581 cgactgggat cccgaacagt tcactgttga ggactccgca ttcggctatg ttgtaatgaa
     2641 gaacggcgcg gttatcagcg ttgaatcaag ctgggcactc aacatcgccg accccgacga
     2701 ggcaaatgtt cttctctgcg gcgacaaggc aggcgccgac acccttcacg acaaggtaag
     2761 aatcaactat gtaaagaata accgtcaggt tatcgaatgt cccgatttct ccgccggcgg
     2821 cgttgctttc tacgacggca aaaaggtttc tcccaaggac cttgaggctc agaagtggct
     2881 cgacgccata gagttcgaca ccgatccctg cgtaacaccc gagcaggctc ttgttgtaac
     2941 acagattctt gaggctatct acacctcggc aaagacgggt cagcccgtat tcttcgaata
     3001 aaagcattta cagtatttga ccttgtaaac gaattacggc ttagccgcgt taagcggtga
     3061 aaagcggaaa ggaaatatac tatggcaatg acactcggaa ttatttcaag ctggtctgag
     3121 gagggcttta agtacgccgc cgatttggga cttaaagccg ttgaattctg ctacaatgta
     3181 ggtcttgacg cggcagagct taaaaagctt gttcccgacc ttaaaaaatg gtccgaaaag
     3241 tacgatgtta aggttctctc catcggcagg tggggcgctc ccaagtttga cgccgaaacc
     3301 ggcgagctta tagaggagga attaaatcac aatttaactc ttctggatgt ttgcgcagaa
     3361 ctcggttgcc ccgtattcaa caccggcgta aactatgttg agagcaaaac atattctgaa
     3421 aatttggaga acgctatcag cttcctaaag aaacttgtcg actacggcaa ggagagaaat
     3481 gttaagatcg ccgtttacaa ctgcgactgg gctaactttg taagg
//
LOCUS       DAONVQ010000155         3458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_18896_length_3458_cov_3.595063, whole genome shotgun sequence.
ACCESSION   DAONVQ010000155 DAONVQ010000000
VERSION     DAONVQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18896_length_3458_cov_3.595063"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PLD98_08370"
     CDS             <1..919
                     /locus_tag="PLD98_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA06295.1"
                     /translation="TAVCTFLVSEKVGVSFTLTVIMLSVVSFFTMLPICIYGQERNYH
                     PEKEEENFSFMLMFKYLIKNKYLLIFYSGYFFCAALNTMGAVTLFASYYLFGSSIFSL
                     LVGALTIAPSLIIALVLPKINKKIDKFVLLIWCNIISVVLGLITYFVGYKNVVAFIIL
                     TVLRSAPLSAASFLNFLFTPDCAEYGQYKTGIDAKGITFAIQTFSAKITGAISSSLAM
                     FLLGLFKWIPVTASDFEELQRLNIQQPPEALSGLWITFILVPTIGTIIGIILYSFYKL
                     NDKDVQIMAKYNAGEIDRETAESMLSRKY"
     gene            complement(1065..2627)
                     /gene="putP"
                     /locus_tag="PLD98_08375"
     CDS             complement(1065..2627)
                     /gene="putP"
                     /locus_tag="PLD98_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HQA06296.1"
                     /translation="MSSDQLQIAIAMGLYMICVIIIGIVYAKRANESTNNYYLGGRSL
                     GPWVTAMAAEASDMSGWLLMGLPGVAYWVGLSDAVWTAIGLLIGTYLNWLFVAKRLRN
                     YSHIAGDAITLPEFLSNRFKEKNKIILTIAALFILVFFSFYAASCFVTVGKLFSTLFN
                     TSYYSMMILGVVFVVLYTLVGGYLAESASDFMQALVMFFALTLTLIVGVVNAGGVSAV
                     IDNAKQIPGFFEFFGIATPALEEGTQIVSGGSPVFGEAGKYGLLTIVSTMSWGLGYFG
                     MPQVLLKFMGIRKSSELKLSRRVATVWCAISLFAAVVIGIIGRVLYPTALLTKTASEN
                     IFVHMSTSFFPPILAGVVMAGILAATISSSDSYLLIASSAFSKNIFKGILKKDADDKT
                     VLRISRAALLLIAIIAAVLAYDESSIIFKIVSFAWAGFGATFGPAVLFSLFWKRTTKE
                     GVIAGMLAGGVMVFVWNKALSKLGGVFSIYELMPSFIISCIFIVVVSLLTKEPSKEIV
                     DEFERVKNYAEE"
     gene            complement(2822..2998)
                     /locus_tag="PLD98_08380"
     CDS             complement(2822..2998)
                     /locus_tag="PLD98_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06297.1"
                     /translation="MKKVLKVIAIIAGIAGAAFAVYVAVTKYLNKKQTIGNPEENYVS
                     CSCFDDDFVTEAVV"
     gene            complement(3091..3351)
                     /gene="rpsT"
                     /locus_tag="PLD98_08385"
     CDS             complement(3091..3351)
                     /gene="rpsT"
                     /locus_tag="PLD98_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009983232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQA06298.1"
                     /translation="MPNIKSAKKRVRIAAVKTARNKSTKSALKTAIKKANAAIDANAA
                     NKVELVNYAFKKIDQAVAKNILHKNNAARKKSALARKLNAAK"
BASE COUNT          956 a          809 c          753 g          940 t
ORIGIN      
        1 cacagcagtt tgcaccttcc ttgtcagcga aaaggtgggc gtaagtttta ctttaacggt
       61 aatcatgctt tctgtggtta gcttctttac catgctgccc atctgcattt acggtcagga
      121 gagaaactat caccccgaaa aagaagaaga gaatttcagc tttatgctga tgtttaaata
      181 ccttattaaa aataaatacc tgctaatctt ctactcggga tacttcttct gcgcggctct
      241 gaacacaatg ggagcggtaa cccttttcgc atcatattat cttttcggca gctcgatttt
      301 cagcctcttg gtcggagccc ttaccatcgc gccttcgtta attattgcgc ttgtgctgcc
      361 caagataaac aagaaaatcg ataagttcgt tcttctcatt tggtgcaata taatcagcgt
      421 tgtgctgggt cttattacct actttgtggg atataagaat gtggtcgcgt ttataattct
      481 taccgtgctg cgctccgctc ctctcagcgc cgcaagtttc ttaaacttcc tctttacccc
      541 cgactgcgct gaatacggtc agtacaaaac cggcatagac gccaagggca ttacctttgc
      601 cattcagact ttctccgcaa agataacggg agccatctcc tcctcccttg ccatgttcct
      661 tctcggcctc tttaagtgga tacccgttac cgcctccgac ttcgaggaac tgcaaaggct
      721 caacatccag cagccccccg aagctctcag cggtctttgg ataaccttta tactcgtgcc
      781 caccatcggc acaataatag gcattattct gtactccttc tataagctta acgacaagga
      841 tgttcagata atggcaaagt acaacgcggg cgaaatcgac agagaaaccg cggagtcaat
      901 gctttcgaga aaatattaaa taatcagccc tcggttttcc gagggcttta ttttggggct
      961 attttcgctg aaaacccctt tcagcattaa aaccctcgga ctcagccgag ggctttatag
     1021 gtttccgcaa aattaaaacc cccgccgtaa gacgggggag ttttttattc ttcagcgtag
     1081 ttttttactc tttcgaactc atccacaatt tcctttgagg gctcctttgt aaggagggat
     1141 acaacaacta tgaatatgca ggaaatgatg aatgatggca taagctcgta aatggagaat
     1201 acgccgccaa gctttgagag tgccttattc catacaaaga ccatgacgcc gcctgccagc
     1261 atgcctgcga ttacgccttc ctttgtggtt ctcttccaga acagtgagaa cagcacagcg
     1321 ggtccgaatg tggcgccgaa gcctgcccat gcgaatgaaa ctatcttaaa gataatgctt
     1381 gattcgtcat aggcaagtac tgccgcgatg atggcaataa gaaggagagc agcccttgat
     1441 attctgagaa cagtcttgtc atccgcgtct tttttcagaa tacccttaaa gatattcttt
     1501 gagaaagcgg aggacgcgat aagcaggtaa gagtcagagg agctgattgt ggccgcgagt
     1561 atgcctgcca ttacaacgcc tgccagaatc ggcgggaaga agctggttga catatgaacg
     1621 aaaatgttct cggacgcggt ctttgtaagc agagccgtgg gatagagcac tctgcctata
     1681 atgccgatta ctacagcggc aaagagggag atggcgcacc atacggttgc gactcttctt
     1741 gagagtttca gctcagagga ctttctgatg cccatgaatt taagcagaac ctggggcatg
     1801 ccgaagtatc cgagacccca agacattgtg gaaacaatgg tcaaaagtcc gtatttgcct
     1861 gcctctccga atacgggtga gccgcccgat acaatctgag ttccctcttc caatgcgggg
     1921 gttgcgatgc cgaagaactc aaagaagccg ggaatctgct ttgcgttgtc aataacagcg
     1981 gaaacgccgc ctgcgttgac aacaccgaca ataagggtga gtgtcagggc aaaaaacata
     2041 accagagcct gcataaagtc ggacgcgctt tcggcaagat agccgcccac caatgtgtaa
     2101 agcacaacaa atacgactcc cagtatcatc atcgaataat atgatgtgtt gaaaagtgtg
     2161 ctgaacagct tgccgactgt tacaaagcag cttgcggcat agaatgagaa gaatacaaga
     2221 ataaataaag cagcgatagt gagtattatc ttgttcttct ctttaaatct gttgcttaaa
     2281 aactcgggga gcgtaattgc gtcgccggcg atatgagaat agtttctaag tctcttagcg
     2341 acaaacagcc agttgagata tgtaccgata agaagtccga tagctgtcca aacagcgtcg
     2401 ctgagaccta cccagtaggc aacgccgggc agacccataa gcagccagcc gctcatgtcg
     2461 gaagcctccg cagccatagc ggttacccac gggccgaggg acctgccgcc aagataatag
     2521 ttgtttgtgc tctcgttagc cctctttgcg taaacaatgc ctattatgat tacgcaaatc
     2581 atgtaaaggc ccatcgcaat tgcgatttgc agttgatcag aactcataaa tttcctccta
     2641 ataccaaggc gaagttatac ttaattgtat tccgcccttc tttaatatat tagcagataa
     2701 aagaaataaa atttcccaac aattatacag tatttggact tttgtgtcta tattttttcg
     2761 tcgaaaataa aaaaattccg ccgatttcgg cggaagggga agtaattgct aaagagcgga
     2821 atcaaacaac tgcctcggtt acaaagtcat cgtcaaagca tgagcaggaa acatagtttt
     2881 cttcggggtt tcctattgtc tgcttcttgt tcaaatactt tgtaacagca acataaacag
     2941 cgaaagccgc acccgctata ccggcaatga ttgcaataac cttcaatacc tttttcattt
     3001 cctgaccatc ctttctaatg accatagctt tatttcaaaa ggcagaaaac aaagaagctc
     3061 ccgccctttg tattttaagt aaagaccgat ttatttggca gcgtttaact tacgggcaag
     3121 agctgacttc tttcttgcgg cgttgttctt gtgaaggatg ttcttggcaa cggcctgatc
     3181 gatcttcttg aaagcatagt ttacaagctc aactttatta gcagcattcg catcaatagc
     3241 ggcattagcc ttcttgatag cggttttaag agctgatttg gtggacttgt ttcttgcagt
     3301 ctttactgcg gcgatacgaa cgcgtttttt cgcagattta atattgggca taattccacc
     3361 tcctttacca tacacaggta ataatattaa cacgaatatt attaaaatgc aagaattatt
     3421 atacagtttt tatttaagtt cacaagcggt aaaatgcg
//
LOCUS       DAONVQ010000156         3346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_19678_length_3346_cov_4.340930, whole genome shotgun sequence.
ACCESSION   DAONVQ010000156 DAONVQ010000000
VERSION     DAONVQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19678_length_3346_cov_4.340930"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLD98_08390"
     CDS             complement(<1..466)
                     /locus_tag="PLD98_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HQA06299.1"
                     /translation="MRIELNQAIEMLNSHDNILILAHRNPDGDALGSGYALCRALRSV
                     GKNAEFKYFDKIPKVFLELEACCENTDFEPSFVVAVDCADSKILGDFPDGRYKEKVDL
                     CIDHHSSNTLYAKYTYLDDNAAATSEIILDIARAIGAEIDTLTAQAIYIGLST"
     gene            complement(488..841)
                     /gene="rbfA"
                     /locus_tag="PLD98_08395"
     CDS             complement(488..841)
                     /gene="rbfA"
                     /locus_tag="PLD98_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQA06300.1"
                     /translation="MPPRKSDHMAEDIKREISAIIRDLKDPRVRESFLTIVRVDFAHD
                     LSLGRIYVSAMEGLESAKRAVEGLESASGYIRRELGLRLKLRKAPELKFIADDSVRSG
                     IELFKKLNEDKKGEE"
     gene            complement(865..3228)
                     /gene="infB"
                     /locus_tag="PLD98_08400"
     CDS             complement(865..3228)
                     /gene="infB"
                     /locus_tag="PLD98_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQA06301.1"
                     /translation="MTTKYRVYEVAKDFGQKSSDIIKLLEQYFEEPKKHQTALEPEEL
                     DVIFDTLTQKYAVESFDEYFASAQKKKAEAEEKADKKEAPEKKEAAAETEKKSEGEKP
                     SAPKDEKAHEQKAKEKKDDKAFEARTKVKRTVDTRITHVQLDKYNEKYDNLASSTTNI
                     RDTETNKQKIKQKSQQYRKKVGTRRHRRETEQERLKRIAEERAKKPITITIPDELTVG
                     ELAMKLKMTAAEVIKKLMQLGMMATINETIDYDTAEIVATELGAKVEREVVVTLEERI
                     IDDSEDDEKDLVPRSPVVVVMGHVDHGKTSLLDAFRNTSVTATEAGGITQHIGAYRVN
                     LNGQEITFLDTPGHAAFTSMRARGAKATDIAILVIAADDGIMPQTVEAINHAKAADVS
                     IIVAINKMDKPDANPEKIKQQLTEYDLVPEEWGGDVICVPVSAKTKMNLDKLLEAILL
                     VAEIKELKANPNRPAKGIVIEAKLDKGRGPVATLLVQNGTLHTGDVIVAGTAVGRVRL
                     MTNDKGQVVTEAGPSVPVEVMGLDETPNAGDTFDAVSDERLARELVEQRKEEQKNAQL
                     SCDRRVTLENLFSTFEEGKVKDLNIIVKADVQGSVEALRQNLEKQSNEEVRVRVIHGG
                     VGAITESDVMLANASNAIIIGFNVRPDPIAASNAERDCVEIRTYRVIYDCIEDVQAAI
                     KGMLAPKYRDVELGRVEVRETFKISSIGTIAGSYVLSGKVTRNSKIRVVRDGIVIAED
                     TIASLKRFKDDVKEVSAGYECGIGLDKFNDIKVGDILEAYIVEEYRD"
BASE COUNT          655 a          903 c          791 g          997 t
ORIGIN      
        1 ccgtcgaaag ccctatataa atcgcctgag cggtaagggt gtcgatttcg gcgcctattg
       61 ccctcgcaat atcaaggatt atctccgatg tcgccgcggc gttatcgtca agataggtgt
      121 atttggcgta cagcgtgttt gaggagtggt ggtctatgca caaatccacc ttttccttgt
      181 atctgccgtc gggaaagtcg cccagaattt tgctgtcggc gcagtcaaca gccacgacaa
      241 aggagggctc aaagtccgta ttttcgcagc aagcctcaag ctctaaaaaa accttgggga
      301 ttttgtcaaa atacttgaat tccgcgtttt tgcccacgga cctgagcgct ctgcacaatg
      361 cgtagccgct gcccaaagcg tccccgtcgg ggttgcgatg agcaagaatc agaatattgt
      421 catggctatt aagcatttct attgcctgat tcagttcaat acgcatattt ttccctttcg
      481 gtaaagatta ctcttcacct tttttatcct cattaagctt tttaaaaagc tctattccgc
      541 tcctgaccga gtcatcggct atgaatttaa gctcgggagc tttccttagt ttcagcctaa
      601 gacccagttc gcgcctaata tagccgcttg cgctctcaag accctctacg gctcttttcg
      661 cgctttccaa gccttccatg gcgcttacat atatccttcc taaggacaaa tcatgtgcaa
      721 aatccactct gactattgtc aaaaaggatt ccctgaccct ggggtccttt aaatctctga
      781 tgattgcgga aatctctctt tttatatctt ccgccatatg gtcagatttt ctgggaggca
      841 taatatcacc tcgattttta cttcttagtc cctgtactcc tcgacaatat aagcctcaag
      901 aatgtctccg accttgatgt cgttgaactt gtcaagacct atgccgcact cgtatccggc
      961 gctgacttct ttgacatcgt ccttgaatct cttgagcgag gcgatggtgt cctctgcgat
     1021 aacgatgccg tcgcgcacaa cacggatttt gctgtttctt gtgactttgc cgctgagcac
     1081 ataggagcct gcgattgtgc cgatgctgga gattttgaag gtctctctga cctcgactct
     1141 tccaagctcc acatctctgt atttgggtgc cagcataccc ttgatggcag cctgcacatc
     1201 ctcaatgcag tcgtaaatga ctctgtaggt tctgatttca acgcagtcac gctccgcgtt
     1261 ggaggcagca atcgggtcgg gacggacatt aaagccgata atgattgcgt tggaggcgtt
     1321 ggcgagcatg acatcggact ctgtgattgc gccgacgccg ccgtgaatga ccctgaccct
     1381 gacttcttcg ttggactgct tttcaaggtt ctgccttaga gcctcaacgg agccctgaac
     1441 atcggccttt acaataatgt taaggtcctt gaccttgcct tcctcaaagg tggagaacag
     1501 gttctcgaga gttacccttc tgtcgcagct gagctgagcg ttcttctgct cttccttcct
     1561 ctgctcgaca agctctctcg cgagacgctc gtcggatacg gcgtcaaagg tatcgccggc
     1621 gttcggggtt tcgtcaagac ccatgacctc aacgggcacg gaggggcctg cctcggttac
     1681 gacctgaccc ttgtcgttgg tcataagcct tacacggccg accgctgtgc cggcgacgat
     1741 aacatcgccc gtatgcagtg taccgttctg aacaagcaag gtggcgacgg gaccgcggcc
     1801 cttatccagc tttgcctcga taacaatacc ctttgcgggt ctgttggggt tggctttaag
     1861 ctccttaatt tcggcaacaa ggagtattgc ctcaaggagt ttgtcgagat tcatctttgt
     1921 cttagccgat acgggaacgc agataacatc tccgccccac tcctcgggga caaggtcgta
     1981 ttcggtaagc tgctgcttta tcttctccgg atttgcgtcc ggcttatcca tcttgttaat
     2041 tgctacaatg atgctgacat ccgcggcctt agcgtggttg atagcctcaa ccgtctgggg
     2101 cataatgccg tcgtcggcgg ctataacaag gattgcgata tccgtagcct ttgcgcctct
     2161 tgcgcgcatg gatgtgaacg cagcgtgacc gggcgtgtca aggaaggtaa tttcctgacc
     2221 gttaagattt accctgtacg cgcctatgtg ctgggtaatg ccgcccgcct cggtagcggt
     2281 taccgaggtg tttctgaagg cgtcaagcag cgaggtcttg ccgtggtcga catggcccat
     2341 gacaacgaca acggggcttc tgggaacaag gtccttctcg tcgtcttcgc tgtcgtctat
     2401 gattctttcc tcaagtgtga ccacaacttc cctttctacc ttcgcgccaa gctctgtggc
     2461 tacgatttcc gcggtgtcgt agtctatggt ttcgtttatg gttgccatca tgcccagctg
     2521 catgagcttc tttataacct ccgcagccgt catcttcagt ttcatggcaa gctcgccgac
     2581 ggtaagctcg tcgggtatgg taattgtaat gggcttctta gctctttcct ccgcgattct
     2641 cttgagtctt tcctgctcgg tctcgcgtct gtgccgacgg gtacccacct tcttgcggta
     2701 ctgctgagac ttctgcttaa tcttctgctt gtttgtctcg gtatccctta tattagtggt
     2761 tgaagatgcg agattgtcgt acttttcgtt atatttgtca agctgaacat gggtaattct
     2821 tgtgtcgacc gtacgcttaa cctttgtgcg agcctcaaag gccttgtcat ccttcttctc
     2881 cttagccttt tgctcgtgag ccttctcgtc cttcggggct gagggctttt ccccttcgga
     2941 cttcttttcg gtctctgctg cggcttcttt cttttcggga gcctcttttt tgtctgcctt
     3001 ttcctcagct tcagcctttt tcttctgagc ggaggcaaag tattcgtcaa agctctccac
     3061 cgcgtacttc tgagtcagcg tatcgaagat aacatcaagc tcctcgggtt caagggcagt
     3121 ctgatgcttc ttaggctctt cgaaatactg ctccaacagc tttataatat cgctggattt
     3181 ttgtccaaaa tccttcgcaa cctcatatac tctgtatttt gtcgtcatat aataagccct
     3241 ccttggttgt ttcgtcggat aacttcaagg caaaaccctc gtcgtttata gatatcactc
     3301 ctgcgcgctt gcctgtggca gaagcgatgt catccattgt cagctt
//
LOCUS       DAONVQ010000157         3299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_20001_length_3299_cov_4.758631, whole genome shotgun sequence.
ACCESSION   DAONVQ010000157 DAONVQ010000000
VERSION     DAONVQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20001_length_3299_cov_4.758631"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLD98_08405"
     CDS             <1..620
                     /locus_tag="PLD98_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HQA06302.1"
                     /translation="AFGIFLTSKGYSPLWAFLMSLTVYAGAMQFVTVELLVSPFSALN
                     AAVMTLTVNARHLFYGISMLKKYRGTGKFKPYLIFSLTDETYAILSTEKVPAGENKGR
                     LYFLVSVFNHLYWLIGSTLGAVFGSTLSIDLKGLDFSMTALFIVVFIEQWKSAEKHLP
                     ALTGVCASLLCLLIFGSKNFIIPSMVLIIIILLAAKNLNEGGEKK"
     gene            617..946
                     /locus_tag="PLD98_08410"
     CDS             617..946
                     /locus_tag="PLD98_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HQA06303.1"
                     /translation="MKNTEALCVVAIVALVTALLRFLPFILFRSEEKTPKTIKYLGKV
                     LPFAATGMLVVYCFKSVTPLAYPYALPELIAAAAVVVLHLIKRNTLFSIAGGTLVYML
                     LVNLVFV"
     gene            complement(978..1559)
                     /locus_tag="PLD98_08415"
     CDS             complement(978..1559)
                     /locus_tag="PLD98_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQA06304.1"
                     /translation="MMPKKTVLLIGDSIRIGYCEKVKEELSDIADVYFPQENCRDTHY
                     VLCSLNGWTKLCDPKEVDIVHFNCGHWDCARFNYADSPLTPLSEYEYNIKRIIDAIRY
                     YYPKAKVFFATTTPVVESRTGDRQAYRSNDDIRAYNAAGVRAANEKGVEVNDLFEFAL
                     PFDEGKYTDFCHYTDEANVLLGQRVARFIRERL"
     gene            1715..2278
                     /locus_tag="PLD98_08420"
     CDS             1715..2278
                     /locus_tag="PLD98_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06305.1"
                     /translation="MKRSLIAVSMAIALLIGIVCGGAMVGYAGKAMFGDIDGNGKVTP
                     ADARLILRAAAKLEDLKEPTVNNASAADTLLYNDNGLKITYKLLIHNERANTKTLYFL
                     IENSTNKLISVRMKGFTVNKKYVISPTFGGFVYDRETVLKSLTLSDNDLKDYDVKKIN
                     TITFKFELSDIYLKPFAASDEITITLP"
     gene            complement(2314..2757)
                     /gene="dut"
                     /locus_tag="PLD98_08425"
     CDS             complement(2314..2757)
                     /gene="dut"
                     /locus_tag="PLD98_08425"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQA06306.1"
                     /translation="MKEVKFVKLHPDAAVPKKATSGSAGFDLRALLSEPLTIEGGKTS
                     LIKTGIAIALPEKELAAFIFARSGLASKHGIAPVNCVGVIDSDYRGEIMVPLINHFDE
                     PYTIQPGERIAQLVIMPVPAVRLTEAESLDETDRGEGGFGSTGKQ"
     gene            complement(2805..3149)
                     /locus_tag="PLD98_08430"
     CDS             complement(2805..3149)
                     /locus_tag="PLD98_08430"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQA06307.1"
                     /translation="MEKITVKLEICGTPYYITTDEDKSYTEALGAELNDRITRILRSG
                     RNLSVTQAAVLAALESLDKQKKAEQSSENLRSQVKDYLEDAAKAKSERDFYKREIERL
                     KGSAADAPPKLW"
BASE COUNT          807 a          838 c          730 g          924 t
ORIGIN      
        1 gcgccttcgg catcttcctc acaagcaagg gctattcgcc tttgtgggcg tttttaatga
       61 gccttaccgt ttacgccggc gccatgcagt ttgtgacggt ggaactgctt gtttccccct
      121 tttcagctct gaacgcagcg gtaatgaccc ttacggtcaa cgcaagacac ctgttttacg
      181 gcatttccat gcttaaaaag tacaggggaa cggggaaatt caaaccctat cttattttct
      241 cgctgactga cgaaacctac gcgattctca gcaccgaaaa agtgcccgcg ggtgagaata
      301 aaggcaggct ctacttttta gtgtcagttt ttaatcacct ttattggctc attggctcca
      361 cgctgggagc ggttttcggc tcaacactga gtattgacct aaagggactc gatttttcaa
      421 tgaccgccct ttttatagtg gttttcatag agcagtggaa aagcgccgaa aagcacctgc
      481 ccgccctgac gggagtgtgt gcctcccttt tgtgcctttt aatcttcggc tcaaagaatt
      541 tcattatccc gtcaatggta cttataatca taatacttct cgccgcaaaa aatcttaacg
      601 agggagggga gaagaaatga aaaataccga ggctctctgc gttgtggcaa tagtcgctct
      661 tgtgacggct ctgctcagat ttctcccctt catccttttc agaagcgagg agaaaacccc
      721 aaagacaata aagtatctcg gcaaggttct gccctttgcg gcaacgggaa tgctggtggt
      781 ttactgcttt aagtcggtaa cgcctcttgc atacccctat gccctgcctg agcttatcgc
      841 cgccgcagcc gtggtggttc tgcatctaat aaagcgcaat acacttttta gcatagcggg
      901 cggcaccttg gtttatatgc tccttgtaaa tctggttttc gtataaaaga aacggcagct
      961 aagctgccgt ttttttgtta cagtctttcc cttataaacc gagctaccct ctgacccaaa
     1021 aggacattcg cctcatccgt gtagtggcag aagtcagtat acttaccttc atcaaagggc
     1081 agcgcaaatt caaacaggtc atttacctcc acgccctttt cgttggccgc cctaactccc
     1141 gcggcgttgt atgccctaat atcgtcattc gagcggtatg cctgcctgtc gccggtgcga
     1201 gactccacaa cgggagtggt ggtcgcaaag aaaacctttg ccttcggata gtaatatctt
     1261 atggcgtcga ttatcctctt tatgttgtac tcgtactcgc tcaatggggt cagcggggag
     1321 tccgcgtagt taaagcgggc gcagtcccag tgtccgcagt taaaatgcac tatgtcaacc
     1381 tctttcgggt cgcacagctt ggtccagccg tttaaagagc acaaaacata gtgggtgtca
     1441 cggcagtttt cctgcggaaa atatacatcg gctatatccg aaagctcctc tttcaccttc
     1501 tcgcagtagc cgattctgat tgagtcgcca atcagcaaaa cggtcttttt cggcatcata
     1561 tctccccttt tttcggttta cccgattgta acacatagga gcttagattt ccatatgatt
     1621 tgcccgcaaa tttatgagcc taaccgccca tctgttgaca ttattttctg attcaatatg
     1681 gtaaactgat tagaaaaaag aaaggagctt aaatatgaaa aggtccttga ttgcggtttc
     1741 gatggctatt gcgcttctta tcggcattgt ctgcggcggg gctatggttg gctatgcggg
     1801 caaagcgatg ttcggcgaca ttgacggcaa cggcaaggtc acacccgccg acgcaaggct
     1861 gatactcaga gccgcggcaa agctggaaga tctgaaagag cctacagtca ataacgcctc
     1921 cgcagcggac actcttttat acaacgacaa cgggcttaaa attacctaca agctgctgat
     1981 tcacaatgag cgcgcaaata caaaaacgct gtatttcctc attgagaaca gtacaaataa
     2041 gctcatatcc gtcagaatga aaggctttac ggtaaacaag aagtatgtaa tctccccaac
     2101 attcggcggc tttgtgtacg accgtgaaac ggtgctcaag agccttactc tttccgataa
     2161 tgatttgaag gactatgatg ttaaaaagat aaacacaatc acctttaaat tcgagctgtc
     2221 ggatatttat ttaaaaccct ttgccgcatc ggatgaaata accatcaccc tcccgtaaca
     2281 aaaacccctt atcccaaagg ataaggggaa tatttattgc tttcctgttg agccaaagcc
     2341 gccctcgccc ctgtccgttt cgtcaaggct ttcagcctcc gtaagacgca cagcgggaac
     2401 gggcatgata acaagctgag ctattctctc gcccggctga attgtgtagg gctcatcaaa
     2461 gtgatttatc agcggcacca taatctcgcc acgatagtcg ctgtctataa cgcccacgca
     2521 gtttacgggg gctatgccgt gctttgaggc aagaccgctg cgggcaaata taaatgcggc
     2581 aagctctttt tcgggcaggg caatcgcaat tcccgtcttt ataagcgagg ttttgccgcc
     2641 ttcaatcgtt aaaggctcac ttaagagcgc gcgcaaatca aagccggctg agccgcttgt
     2701 tgcctttttc ggcaccgcgg cgtccgggtg gagttttacg aattttactt ccttcataat
     2761 tatcctttca aaaataaagg ccgcctattg ggcagccctt tttattacca gagttttgga
     2821 ggagcgtcgg ccgccgagcc cttaagcctt tcgatttccc tcttgtagaa atctctttca
     2881 gactttgcct ttgccgcgtc ctcaagatag tcctttacct gagagcgcag gttctccgag
     2941 ctctgctcag cctttttctg cttatcaaga gattcaaggg ccgccagaac cgctgcctga
     3001 gttacggaaa gatttcttcc gcttcttaat atccttgtga ttctgtcatt cagttccgcg
     3061 ccgagagcct cggtgtagct cttatcctca tccgtcgtta tataatacgg agtcccgcag
     3121 atttccagct tgaccgtaat tttttccatg cgtttttctc tccttaatct ctgataggta
     3181 aggccctgac cctgattgcc gttattttat cctatgcaca gtatgtccaa actatgattt
     3241 aatcaaaata atttcacttc tatattaccc gaatttataa aaaaggcaat aattatgtt
//
LOCUS       DAONVQ010000158         3286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_20101_length_3286_cov_4.131848, whole genome shotgun sequence.
ACCESSION   DAONVQ010000158 DAONVQ010000000
VERSION     DAONVQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20101_length_3286_cov_4.131848"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1205
                     /locus_tag="PLD98_08435"
     CDS             96..1205
                     /locus_tag="PLD98_08435"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQA06308.1"
                     /translation="MIKYPRVKNGIISRTEGNIFSYHGWGSVCKATNGELLAVCSAFR
                     MKHVCPFGKTALYRSTDNGLTWSAPTIINDTLLDDRDAGIISLGGDRLLVTWFIHPPM
                     VYTRKYRESILNDCNELEKPITEAHLNYWEAKYTDPTLGGSFIRISEDLGRTWGETIR
                     IPISAPHGPTMRSDGSLIYFGKELYSFGAEKEHALAVYVSDNEGKRWKRLSYIENPEG
                     FKGCHVHEPHIIELKDGTHLGAIRVEGDVVPYRFTVYTTKSKDGGKTWSPLKPTEIEG
                     SPPHLFQHSSGAVLLSVGHRSKPYGQKVYVSYDGGDSFEDVYIIRDDAPDDDLGYPAT
                     IELDDGSLITVYYQRYHSDIKTSFLYSKWRLDDIR"
     gene            complement(1239..2471)
                     /locus_tag="PLD98_08440"
     CDS             complement(1239..2471)
                     /locus_tag="PLD98_08440"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQA06309.1"
                     /translation="MEKKEIKKVVLAYSGGLDTSIIIPWLKENYDNCEVIAVSADVGQ
                     GSELDGLEEKAIKTGASKLYIEDLKQEFVENYIFPTLKAGAVYEGEYLLGTAFARPII
                     AKRIVEIAKKEGADAICHGCTGKGNDQVRFELTIKAFAPDMKIIAPWRIWDIKSREEE
                     IEYAEKHNVPLKISRETNYSKDKNIWHLSHEGLDLEDPANEPQYNKEGFLEMCVSPEQ
                     APDKPEYITIGFEKGVPVSVNGERLSPVELIEKCNEIGGRNGIGLVDLVENRLVGMKS
                     RGVYETPGGTILYRAHKVLESLCLDRETSHFKEQIAIKYAELVYYGQWFTPLREALAA
                     FVDKTQETVTGEVMLKLYKGNIINAGTTSPYSLYDAEVATFDKDHVYNQKDAEGFINL
                     FGLPITVAARLKEKREGK"
     gene            complement(2528..>3286)
                     /locus_tag="PLD98_08445"
     CDS             complement(2528..>3286)
                     /locus_tag="PLD98_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522816.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HQA06310.1"
                     /translation="DAEGGDAVKLNIMKILNEKYGITEVDFRRAELEIVPAFDPSDIG
                     FDRSMIGAYGHDDRVCAYPALMAILDSKEPEHTIMTVLTDKEETGSDGNTGLNSAYLK
                     YFIYDLAAMEGVPGYRVLSKSECLSADVNAGFDPTYPEVMERNNSAYLNRGVVLTKYT
                     GSRGKGGTSDASAEFMGKVISLFERAGIPWQTGELGKVDEGGGGTVAKYVANLDIDVV
                     DVGVPVLSMHSPYEIVSKLDLYAAYRAFYEFFKD"
BASE COUNT          801 a          810 c          807 g          868 t
ORIGIN      
        1 atgctgtacg caagctccga cattgatatt gcggatttgt acgcagagca tatagcgaat
       61 acattggctg tcagcactaa taagggggat aatctatgat aaagtacccg agagtaaaaa
      121 acggcattat aagccgcact gagggcaata ttttttcata tcacggctgg ggttctgttt
      181 gcaaggctac gaacggagag ctgcttgcgg tctgctccgc gttcagaatg aagcatgtct
      241 gcccattcgg caaaaccgct ctttacagga gcacggacaa cggtcttact tggtcagcgc
      301 cgactatcat taacgatacc ctgcttgacg acagggacgc gggcataatt tcccttggcg
      361 gcgacaggct gcttgtaacc tggtttattc atcctccgat ggtctataca agaaaatacc
      421 gcgaatccat acttaacgac tgcaatgagc tggaaaagcc aattacagag gcgcatttaa
      481 actactggga ggcaaaatac accgacccga ctttgggcgg ctcatttata agaatatccg
      541 aggatttggg caggacatgg ggcgaaacaa tcaggatacc catatccgct ccccacggac
      601 ctacaatgag gtctgacggc agcctgattt acttcggcaa ggagctatac tctttcggcg
      661 cggaaaaaga acatgccctt gcggtctatg tttctgacaa tgagggcaag agatggaaaa
      721 gactatctta tatcgaaaac cccgaaggct ttaagggatg ccatgttcat gagccgcata
      781 taattgagct taaagacggc acgcatctgg gagcaataag agttgagggc gatgtggtgc
      841 cctatcgctt tacggtatat acaaccaaat caaaagacgg gggcaaaaca tggtcgccac
      901 tgaagccaac agaaattgaa ggctctcccc cgcatttgtt ccagcattca tcgggagcgg
      961 ttctccttag cgtcggccac agaagcaagc cttacggtca aaaggtttat gtaagctacg
     1021 acggcggcga cagctttgag gatgtatata tcataaggga cgatgccccc gatgacgatc
     1081 tgggctaccc cgccacaatc gagctggacg acggctcgct gattaccgtc tattatcagc
     1141 gctaccacag cgacattaaa acctccttcc tctactccaa atggaggctg gatgatataa
     1201 gataaaaaaa gggagcagat ttaatctgct cctttatgtt atttgccttc tcttttctct
     1261 ttgagccttg ccgcgacggt gatgggcaga ccaaagagat tgataaagcc ttcagcgtct
     1321 ttttgattgt agacatggtc cttatcaaag gttgcgacct ctgcgtcata gagtgagtag
     1381 ggagaggttg tgcccgcgtt aatgatgttg cccttgtaga gcttgagcat tacttcgccc
     1441 gttacggtct cctgagtctt gtcaacaaag gccgcaaggg cttctctaag tggtgtgaac
     1501 cactgaccgt agtaaacaag ctcggcatac tttattgcaa tttgctcctt aaagtggctt
     1561 gtttccctat caaggcaaag gctttcgaga accttatgtg cgcggtaaag aatcgtgccg
     1621 ccgggggtct cgtatactcc ccttgacttc attcccacaa ggcggttttc cacaaggtca
     1681 acaagaccga tgccgttcct gccgccgatt tcgttgcact tttcgattag ctcaacgggt
     1741 gacagtctct ctccgtttac gctgacggga acgccttttt caaagccgat tgtgatatac
     1801 tcgggcttgt cgggagcctg ctcgggcgat acgcacattt caaggaagcc ttccttgttg
     1861 tactgaggct cgttagcggg gtcctcaagg tcaagacctt cgtgtgaaag gtgccagata
     1921 ttcttatcct ttgaatagtt ggtctccctg ctaatcttta agggaacatt gtgcttttcg
     1981 gcgtattcga tttcttcctc gcgggacttg atatcccaga ttctccacgg agcgattatc
     2041 ttcatatcgg gagcaaaggc tttaattgta agctcaaaac gaacctggtc gttgcccttg
     2101 cctgtgcagc cgtggcagat ggcgtccgcg ccctcttttt ttgcgatttc gacaattctc
     2161 tttgcgatta tcgggcgggc aaaggctgtg ccgaggagat actctccctc ataaacagcg
     2221 cccgctttaa gggtggggaa gatataattt tctacaaact cctgctttaa atcctctata
     2281 tacagctttg aggctccggt tttaattgct ttttcctcaa gaccgtcaag ctcggaaccc
     2341 tggcccacat cggcggaaac cgctataacc tcgcagttgt catagttttc tttaagccaa
     2401 gggattataa ttgaggtgtc aagaccgcca gagtaggcaa gaacaacttt tttaatttct
     2461 ttcttctcca tattaccgcc ctctattatg tatttgttaa gagctgtgag tacagctctt
     2521 aacttattta gtctttaaag aactcataga atgctctgta tgcagcgtaa aggtcaagct
     2581 ttgagacaat ctcatagggt gaatgcattg aaagtacggg tacgccgaca tccacaacat
     2641 caatgtcgag gttagcaaca tacttggcaa ccgttccgcc gccgccctcg tctaccttgc
     2701 caagctcgcc ggtctgccag gggattcccg ctctttcaaa cagggagatt accttgccca
     2761 tgaactcggc tgatgcgtct gatgtgccgc ccttgcctct tgagcccgtg tactttgtga
     2821 gaaccactcc cctgttcagg tacgcggaat tgttgcgctc cataacctcg gggtaagtcg
     2881 ggtcaaagcc tgcgtttaca tcggctgaga gacactcaga cttggaaagg actctgtagc
     2941 cggggacacc ctccatagcg gcaaggtcgt agataaagta tttaagatac gcggaattaa
     3001 ggcccgtatt gccgtcgctg cctgtttctt ctttgtcggt aaggacagtc atgatagtat
     3061 gctcgggctc ctttgagtca aggatagcca taagcgcggg atatgcgcag actctgtcgt
     3121 catgaccgta ggcgccaatc atactgcggt caaatccgat gtcggagggg tcaaacgcag
     3181 ggacaatttc gagctccgcg cgtctgaaat caacctcagt gattccgtac ttttcgttga
     3241 ggattttcat aatattgagt tttacggcgt cgccgccctc tgcgtc
//
LOCUS       DAONVQ010000159         3164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_21027_length_3164_cov_4.316500, whole genome shotgun sequence.
ACCESSION   DAONVQ010000159 DAONVQ010000000
VERSION     DAONVQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21027_length_3164_cov_4.316500"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PLD98_08450"
     CDS             <1..888
                     /locus_tag="PLD98_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQA06311.1"
                     /translation="ENPYFYVCPAVLSRCFVFEFKAVTAAEAKEAVRKAFAFLEKERG
                     ESYSIEDGVIEHIAAASGGDVRRAVNSAEMAALSALPDKENPKHKSISLDGVQRLFDK
                     SLIRYDREGDEHYDLLSAFQKSMRGSDPDAALHYLARLLEAGDLPSAARRLMVTAAED
                     VGLAYPMIIPIVKAAVDMAFQVGLPEARIPLADAVVLVCNSPKSNSAYLAIDAAISDI
                     RKGKSGPIPRALQNMHYDGEDAAVKGQFYKYPHDYEGHYVPQQYLPDTLKGVKYYEYG
                     DNKNEQAAKEYWDKIKKRK"
     gene            complement(1134..1406)
                     /locus_tag="PLD98_08455"
     CDS             complement(1134..1406)
                     /locus_tag="PLD98_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06312.1"
                     /translation="MIKGVNRQVVDVLETGSEYFERALFFVKPEFSYVSEGKLRENAR
                     QILKNASAPPSTRTKRGKMYYFLKVLKYCAAAAGGGAATLILSAVL"
     gene            1992..2831
                     /locus_tag="PLD98_08460"
     CDS             1992..2831
                     /locus_tag="PLD98_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HQA06313.1"
                     /translation="MNIARNTFGLRLTDFGGINLGLTLDCGQCFRWKKLADGAWKGVV
                     GSHAVRIIEDGEDLIFLGMSEDVFINTFYHYFDFDRDYDKALKRLSEDELLKDTIERY
                     GTIRILNQEPWETLCSFILSSCNNIARIKGIIERLCALFGEDLGGGEFSFPSAERLAV
                     LEPEDLDPVRCGYRAEYVIDAARKVAEGEIDLEKLKVTPLDDARRELMKIKGVGVKVA
                     DCTLLFGLGHMSAFPKDRHILRIAKSAYPDGLPECVKGLEGLAQQYMFHFQRTENGRR
                     TAV"
BASE COUNT          780 a          753 c          864 g          767 t
ORIGIN      
        1 gagaacccct atttttatgt ttgccccgcg gttttaagcc gctgctttgt ctttgagttt
       61 aaagcggtga ccgcagccga ggctaaagag gcggttcgga aagcgttcgc ttttctggag
      121 aaggagaggg gagagagcta ctccatagag gatggggtca tagagcacat agcggcggcg
      181 tcgggcggag atgtaaggcg agccgttaac tccgccgaga tggcggcttt gtccgctctg
      241 ccggataaag agaacccgaa gcataagagt atcagccttg acggcgtaca gcggcttttt
      301 gacaagagcc ttataaggta cgacagggag ggggacgagc attatgacct gctctccgcc
      361 tttcagaagt ccatgcgggg ctccgacccc gacgcggctc tgcactatct ggcaagactg
      421 cttgaggcgg gagacctgcc ctcagcggca agaagactta tggtgaccgc cgccgaggat
      481 gtgggacttg cgtacccaat gataattccg atagtaaagg ctgccgtgga catggccttt
      541 caggtaggtc tgcccgaggc aaggattccc cttgccgacg cggtggttct ggtctgcaat
      601 tcccccaagt ccaactcagc gtaccttgcc atagacgcgg ctataagcga tataagaaaa
      661 ggaaagagcg gccccatacc gagagccctt cagaatatgc actatgacgg cgaggacgcc
      721 gctgtaaagg ggcagtttta caagtacccc cacgactacg agggacatta tgtgcctcag
      781 cagtatctgc ccgataccct aaagggcgta aaatactacg agtacggcga caacaagaac
      841 gaacaggcgg cgaaggagta ttgggataag ataaagaaaa gaaaataatc aggaaaagcc
      901 atagcggagg agcgccccgc cccgaaatac acccgttcaa cagggagcgg ctcactcaga
      961 aatggggtct cccgcccgga gggagcggac accccgcccc gccgatggtt ttgcgccccg
     1021 caaaatgcgg catgcccaca cagcgagcga caccccaccc ggcgccgagc gggctcaaag
     1081 gcacaaaaaa acagaccccc taaggggtcc gatatgcgga aatttattca aagctacaaa
     1141 acagcggaga gtatcagtgt cgccgccccg ccgcctgcag cggccgcgca gtatttaagc
     1201 acctttaaaa aatagtacat cttgcccctt tttgttctgg tcgaaggcgg ggctgaagcg
     1261 ttcttcaaaa tctgccgggc gttctccctc agcttgcctt cgctcacata ggagaactcg
     1321 ggttttacaa aaaacagggc tctctcaaaa tactcgctgc ccgtctccaa aacatcaaca
     1381 acctgcctgt tgactccttt tatcactcgg taccctccca gcattttgtg tttggcattc
     1441 ctattgttgc cttttttaac ttgcctattc cgaaaaggga aagaaataca gttaaaatct
     1501 gtgtgtaatt ttttggaaaa ccgggtcaga aatcgaagaa taaatatgat atttttatgc
     1561 ggaaaccggg gatttttgac aaatcgggga actttcaatc tttgacaaac gatggcaaat
     1621 ttttacattt ttctttcctc gcaacaaaat gtttgttttt caattgcaaa aattttccgc
     1681 gggataatta tacgctctgt ataatgagtt tttgaataaa attgttcaaa actccgtcga
     1741 aagtgtctat aactaagcta aaattcattg atatgggggc attttttcaa aaatcttcta
     1801 attgttcata acttttagac gcctgtttcc atatactacg ggttttaata actttacaga
     1861 ttatactctt tggaataaaa tttgcataaa aaatttactt aaaaattgtt aggtttttct
     1921 attgattttt tctgatattg agtaatttga aggcaaattt atgcatagat ttttaggttg
     1981 gaggaaaatt cttgaacata gcaagaaaca ctttcggact gcgtttgacc gatttcgggg
     2041 gaattaatct gggtcttacc ctcgactgcg gtcagtgctt cagatggaaa aaactcgccg
     2101 acggcgcatg gaagggcgtt gtcggctccc acgctgtcag gataatcgag gacggcgagg
     2161 accttatttt cctcggaatg tcggaagatg tatttataaa taccttttat cactatttcg
     2221 actttgacag ggattatgac aaagctctga aacggctttc cgaagacgag cttttaaagg
     2281 ataccattga gcgatacggt accatcagga ttttaaatca ggaaccgtgg gagacgctgt
     2341 gcagctttat actgtcctcc tgcaacaata tcgcccgcat caagggcata atcgaaaggc
     2401 tttgcgccct tttcggcgag gatttgggcg gaggggagtt ttcgtttccc tctgccgaaa
     2461 ggctggcggt tttggagccg gaggatttgg accccgtaag gtgcggctac agggcggagt
     2521 atgtcattga cgccgcaagg aaagtcgccg agggcgagat tgatttggag aagctgaaag
     2581 taacgcccct tgacgacgcc cgcagggagc ttatgaaaat caagggcgtg ggcgttaagg
     2641 ttgcggactg tacgctgctt ttcggtctcg ggcatatgtc cgccttcccg aaggacaggc
     2701 atattttaag gatagcaaag agtgcctacc ccgacggcct gcccgagtgc gttaagggtc
     2761 ttgagggact cgcccagcag tatatgttcc acttccagcg caccgaaaac ggcagaagaa
     2821 cggcggttta gagcagatac agaatacata aaaagcaggc tcgtgcctgc tttttttgat
     2881 gcgccgcccg tgggagcgtg gctgtccccc ttttttatgg ttcgtccgtc gggctgcgac
     2941 gagcacttac ctgcggtcgg ttggtcggcg ggggttcaac cttgaggggt ggggcggcac
     3001 ggcggtggcg ggcttcactc gcttgggctg tgctatcacc ttggcgcacc cctgcggcgc
     3061 ggcgggcggg gcggttgcct ctcggcgata cgcccaacga tgggtggggc ggtggtcggt
     3121 ttttggttgg cgggtgcgcc cttctgcgca aggtgtggcg ggca
//
LOCUS       DAONVQ010000160         3137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_21243_length_3137_cov_5.357884, whole genome shotgun sequence.
ACCESSION   DAONVQ010000160 DAONVQ010000000
VERSION     DAONVQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21243_length_3137_cov_5.357884"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLD98_08465"
     CDS             <1..502
                     /locus_tag="PLD98_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQA06314.1"
                     /translation="ILVNNAGIIRRADFVDFTEQDWDDVINLNLKMLFKLSQLAARQF
                     IKQGTGGKIINIASMLSFQGGIRVPSYTASKSGVKGLTMAMANELAKYNINVNAIAPG
                     YMITNNTEALRKDEKRSAEILARIPAGRWGLPEDLAGACVFLASSASDYVNGFTLAVD
                     GGWLAR"
     gene            521..1279
                     /locus_tag="PLD98_08470"
     CDS             521..1279
                     /locus_tag="PLD98_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQA06315.1"
                     /translation="MTYARLMQNGSIFYAAYIDGSYYKIDGDLFAEHKVTDKKIEGEF
                     KILAPVSPSKIIALGVNYRNHAAEMGEEVKSDPLIFMKPPTAVIAPYENIEIANPENR
                     TDYEAELAVIIKSKCSRISADEAENYILGYTCANDVTDRVLQKKDGQWVRAKGFDTYC
                     PLGPHIVTGISPMGLRVQSILNGEVRQDSTTDKMINNVFDTVAFISNIMTLLPGDVIM
                     TGTPEGIGPLSAGDEIEIRISGVGSLVNKVVNRE"
     gene            complement(1481..>3137)
                     /locus_tag="PLD98_08475"
     CDS             complement(1481..>3137)
                     /locus_tag="PLD98_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQA06316.1"
                     /translation="VGPTLSGRAIDAIELGKGKVDFRTVFFYASLMICFYILSSVMSY
                     VLSIIMIKLSRSVVYRMRRDVFDRLTRLPVSFFDRHQTGEIISIISYDIDTINASLST
                     DLLHIATSLITVVGSLVMMLTICPPLVLVFTVTIPASILFTRYMSKRVRPLYRKRSIK
                     LGELNGYIEEITSGLKTIKAYNREDVMINRFDEHNLDAVDAYFETEYYGSVTGPSVNF
                     INNISLALVSVFGSLLYLAGGISLGDISSFVLYSRKFSGPINEFANIISELQSAFAAA
                     ERVFRLMDELPEPEDAPDALTLTDVRGDVRFDHVTFGYENGKTILRDLNFTADSGKLI
                     AIVGPTGAGKTTIINLLMRFYDINEGSITVDGHDIRDVTRASLRRAYTMVLQDTWLFH
                     GTIFENIAYGKDGATLAEVEAAAKAAKIDGYIKRLPNGYNTVLSDNGVNISKGQKQLL
                     TIARAMLIDSKMLILDEATSNVDTQTERKIQAAMRSMMQGKTCFVIAHRLSTIRDADL
                     ILVMRDGQIVETGTHDSLIAANGFYASLYRSQFDREEDLKAVQ"
BASE COUNT          848 a          744 c          781 g          764 t
ORIGIN      
        1 tattttggta aacaacgcgg gcattatccg cagagcggat tttgttgatt tcacggagca
       61 ggactgggac gatgttataa atctcaacct caaaatgctc tttaaactca gccaattggc
      121 agcaaggcaa tttattaagc agggcacagg gggcaagatt atcaatatcg ccagcatgct
      181 ctctttccag ggcggaattc gtgttccaag ctatacggca agcaaaagcg gcgttaaggg
      241 tctgactatg gcgatggcga atgaactcgc taagtacaat ataaatgtga acgcaatcgc
      301 tcccggctat atgataacga acaataccga agctctgcgc aaagacgaga agcgcagcgc
      361 ggaaattctg gcgcgcattc ccgcaggccg ctggggtctg cccgaagatt tagccggtgc
      421 ctgcgtattc cttgcaagca gcgcgtcaga ctatgtaaac ggctttaccc ttgcggttga
      481 cggcggatgg ctcgcccgtt aaaaatagga ggacaaactt atgacttatg caagacttat
      541 gcaaaacggc agtatttttt atgcagcata catagacgga agctattata aaattgatgg
      601 agaccttttt gccgagcata aggtgaccga caaaaagata gagggggagt ttaagatatt
      661 ggcgcccgta tccccatcaa aaataattgc tctgggcgtg aattacagaa atcacgcggc
      721 ggaaatgggc gaagaagtaa agtcagaccc cctgatattt atgaagccgc ccactgctgt
      781 aatagctccg tatgagaata tagaaatcgc gaatcccgag aaccgcacgg actatgaggc
      841 tgagcttgcg gtcattataa agagtaagtg cagcagaatc tcggccgacg aggctgaaaa
      901 ctatatttta ggctatacct gcgcgaacga tgtaaccgac agagttctgc aaaagaaaga
      961 cgggcagtgg gtcagggcaa agggctttga tacatattgc cccttgggtc ctcatattgt
     1021 tacgggcatt tcgccgatgg gccttcgtgt gcagtccatt ctgaacggcg aagtgcgtca
     1081 ggactccact acggacaaaa tgataaacaa tgtttttgat accgttgcct ttataagcaa
     1141 tataatgacc ctgctgccgg gtgatgttat tatgacaggc acccccgagg gaatcggccc
     1201 gttaagcgca ggagatgaaa tcgaaatcag aatttcaggc gtaggcagcc tcgtaaacaa
     1261 ggtcgtaaat cgtgaataaa tttaaggctc taacgcagcc aagctcccat gtttataccc
     1321 gaacccctga aactggcatc cgaaaaaaca ctggttgcat caatataaaa cccttttaaa
     1381 agaaaaaccc cgaagccttt ttggcttcgg ggttttgttc gagagacggg acttgaatgc
     1441 tcattaaaag cccctgtgaa atcgaatgtt tatttatctc ctactgcacg gcttttaaat
     1501 cttcttctcg gtcaaactgc gagcggtaca gagaagcgta aaagccgttg gcggcaatca
     1561 atgaatcgtg agtgcctgtc tcaactattt gcccgtcgcg catgacaagt ataaggtcgg
     1621 cgtcgcgtat ggtggaaaga cggtgcgcga ttacaaagca ggtttttcct tgcatcatag
     1681 agcgcatggc cgcctgtatt ttccgctccg tctgtgtatc aacattcgag gtagcctcgt
     1741 cgagaatcag catttttgaa tctatcagca tggcgcgcgc tattgttaaa agctgcttct
     1801 gacccttaga gatatttact ccgttgtcgc tcagaaccgt gttataaccg ttcggcaagc
     1861 gcttaatata accgtcaatc tttgcggcct tcgcggctgc ctcgacctcg gcaagagtag
     1921 cgccgtcttt gccgtacgcg atattttcga aaatagtgcc atgaaagagc catgtgtcct
     1981 gcagcaccat tgtgtaagct ctgcgaagac ttgcccgtgt tacatcacga atgtcgtggc
     2041 catcgacggt tatgcttcct tcgtttatgt cataaaaccg catcagcaaa ttgatgatag
     2101 tcgtttttcc tgctccggtc gggccgacta tggctatcag ctttccgctg tccgccgtga
     2161 aattcaaatc gcggagtatg gtttttccgt tttcataacc gaaggtcaca tggtcaaagc
     2221 gcacatctcc ccgcacatcg gtaagcgtca gcgcatcggg agcatcttcc ggttcgggga
     2281 gctcatccat aagcctgaat acacgctctg ccgccgcaaa ggctgactga agctcactga
     2341 taatgttcgc aaactcattt ataggcccgg aaaatttacg ggaatagagc acaaacgatg
     2401 aaatatctcc cagcgaaatt ccgcctgcca ggtatagcag agaaccgaat acgctcacca
     2461 gcgcgagaga aatgttgtta atgaaattaa ccgagggacc tgttacgctt ccgtagtact
     2521 cggtttcgaa atatgcgtca accgcatcga ggttgtgctc gtcgaagcgg tttatcatga
     2581 catcctcgcg gttgtaggct ttgatggtct ttagaccgct ggttatttcc tctatatagc
     2641 cgttcaactc gccgagcttt attgaccgtt tacggtaaag cgggcgtaca cgcttggaca
     2701 tatatcgggt gaacagtatc gacgcgggga ttgtcacggt aaagaccaga acaagcggcg
     2761 gacaaatcgt cagcatcata accaacgaac cgacaaccgt tatcagactg gtcgctatat
     2821 ggagcaggtc ggtcgacagc gaggcgttta tcgtatctat atcgtaggaa atgatgctga
     2881 ttatctcacc cgtctgatgg cggtcgaaaa aggaaacggg cagacgggtc aaacggtcga
     2941 atacatcccg tctcatgcgg tagacgacgc tgcggctcag ctttatcatt atgattgaca
     3001 gtacatagct cataaccgag gataagatat aaaagcatat cataagcgaa gcgtaaaaga
     3061 acaccgtgcg gaagtctacc tttcccttgc ccagttcaat cgcgtcaata gcgcgtccgg
     3121 acagcgtagg tccgaca
//
LOCUS       DAONVQ010000161         2987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_22610_length_2987_cov_5.190314, whole genome shotgun sequence.
ACCESSION   DAONVQ010000161 DAONVQ010000000
VERSION     DAONVQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22610_length_2987_cov_5.190314"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLD98_08480"
     CDS             complement(<1..543)
                     /locus_tag="PLD98_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HQA06317.1"
                     /translation="MAKQILYGEEARKALLTGIDKLSNTVKITLGPKGRNVVLDKKYG
                     SPLITNDGVTIAKEIELEDPFENMGAQLVKEVATKTNDVAGDGTTTATILAQALVREG
                     MKNVAAGASPMAVKRGIQMAVDKAVEALKKSSKPVSSNADIARIATISAGDEKIGDLI
                     AEAMAKVTSDGVITIEESKTM"
     gene            complement(564..848)
                     /locus_tag="PLD98_08485"
     CDS             complement(564..848)
                     /locus_tag="PLD98_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQA06318.1"
                     /translation="MTLKPLGDRVVVKFVEAEETTKGGIILAAAAQEKPQIAEIIAVG
                     PGGIVDGKDVIMEVEEGDRVILSKYAGTEVKLGNEEYVIVRQSDILAIVE"
     gene            1486..2829
                     /gene="mgtE"
                     /locus_tag="PLD98_08490"
     CDS             1486..2829
                     /gene="mgtE"
                     /locus_tag="PLD98_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQA06319.1"
                     /translation="MTMTISMIAQLLEEKKYNSLYEQLNTMNAVDIAEIMDELQSEQA
                     VRVFRLLSKDLAAEVFANLPSDKQEFLIGCLTDAELERVTSDMFIDDTVDLMHEMPAT
                     IVKRILRVANPADREVINELLNYPENSAGSIMTPEFMDLRENMTVGEAIEKIRREGID
                     KETIYNCYILDNSRILKGVITVKDLLIAPLDTVVGDIMETNIISVHTLSDRESAVMAM
                     KKYGLMTLPVVDSENRLVGIITIDDAVDVIVDESSEDIALISAVNPSDAPYLKTSVVR
                     HAFNRIPWLLLLMVTSIITGLIISRYEEAFTAVPLLVTFLPMLMDTGGNSGSQASALI
                     ISSLATGELSPKDYFKVLWKEFRISIICSIALAVANGIRIFVQYGNWQIAMVVSLTLA
                     GTVVCAKIIGCTLPMLAKRLGLDPALISSPFITTMVDAFSVFLYFNIAIAILGKL"
BASE COUNT          767 a          714 c          668 g          838 t
ORIGIN      
        1 catggtcttg gactcctcga tggtgataac gccgtcggag gttaccttag ccatagcctc
       61 tgcgataagg tcgcctatct tctcgtcgcc tgcggagatg gtggcgattc ttgcgatgtc
      121 agcgttgctg gatacgggct tggagctctt cttcagagcc tcgacagcct tgtctactgc
      181 catctgaatg cccctcttaa ctgccatcgg gctagctccg gctgccacat tcttcatgcc
      241 ctctcttaca agagcctgag cgagaattgt agctgtggtt gtaccgtcgc ctgccacatc
      301 gttggtcttt gttgcgactt cctttacaag ctgagcgccc atgttctcaa aggggtcctc
      361 aagctcgatt tccttggcta ttgtaacgcc gtcgttggtg ataaggggtg aaccgtattt
      421 cttatcaaga accacattgc gtcccttggg accaagggtg attttaactg tattgctgag
      481 tttgtcgata ccggtcagca aagccttgcg tgcttcttca ccgtaaagaa tctgttttgc
      541 cataactgtc ctcctcgtct gaattattca actattgcaa gaatatcgct ctggcgaact
      601 ataacatatt cttcgttgcc gagctttact tcggtgcctg cgtatttgct gaggattact
      661 ctgtcgcctt cctcaacctc catgatgaca tccttgccat caacgattcc gccgggaccc
      721 acagcgataa tctcagcaat ctgaggcttc tcctgtgcgg cagcagcgag gataattcct
      781 cccttggtgg tttcttctgc ttcgacgaat ttaacgacaa ctctgtcacc aagcggttta
      841 agtgtcattc tgattcctcc cgattaatat ataatagtat tgtttttgca tcaatttagc
      901 actcttatat ttagagtgct aaaaactatt gtatccatct gctggaaaaa atcaatacct
      961 ttttttaaaa ttcacagaat cgtaataatt attggcagcg ctttattttt tatgccatta
     1021 ttttctaaat gtgaatgcgt ctttaaataa aaaaagcttt ttaaagctcc tcaaaagaaa
     1081 tattaagaat gctttaagat tgtcccttta gcttgacaca ctaagcttta aggaatacaa
     1141 ttaaaatgtt aaagaagcgc catttttctt ctttaacagg cagaggattg ccgcttttaa
     1201 atgcaaaggc ttttgcaaat aaaagcgagt ttgcaacaaa tactatagaa aggagatgca
     1261 gttatggacg atggcagtcg cggtgaccat taccgaagtt gcttagcagg caaaatacgc
     1321 acatcgggca gaaagaccat agctgtttct ttcgtttcta tgtatttgct ttctgcctta
     1381 aaaggaaagg caatgaaaaa atcattgcca taaaagattt gtgcctttgt ctgtgctgct
     1441 ttggtttgtc ccgcattgct ttatccttaa tgcagggtaa agattatgac aatgacaatt
     1501 tcaatgatag cacagctttt ggaggagaag aagtacaact ccctctacga acaactgaat
     1561 accatgaatg ccgttgacat agcggaaata atggacgaac ttcagtctga gcaggcagtc
     1621 cgcgttttcc gccttttgtc aaaggatttg gcggcggagg tattcgctaa tcttccgtcg
     1681 gataagcagg agttccttat aggctgtctt accgacgcgg agcttgaaag agttacctcc
     1741 gatatgttca ttgacgatac cgtcgactta atgcacgaaa tgccggcaac catagtaaag
     1801 agaattctga gagtggctaa ccccgctgac agagaggtaa tcaacgagct tttgaactac
     1861 cccgaaaaca gcgccggcag cattatgact cccgagttca tggacctaag agaaaacatg
     1921 acagttgggg aggcaatcga aaaaatccgc cgcgaaggta tcgacaagga aaccatctac
     1981 aactgctata tactggacaa cagcaggata ttaaagggcg tcattacggt aaaagatttg
     2041 ctcatagctc cgctggatac ggttgtcggc gatataatgg aaaccaacat aatttccgtt
     2101 cacactctct ccgacaggga atccgccgtc atggcgatga aaaagtacgg tcttatgacc
     2161 ctccccgtcg ttgactccga aaaccgcctt gtgggcataa tcaccataga cgacgcagtg
     2221 gatgtcatcg ttgacgagag cagcgaggac atagcgctta tttctgcggt taatccgtct
     2281 gatgccccct atcttaaaac ctcagtggtg cgccacgcct ttaacagaat accctggctg
     2341 ctgctcttaa tggtaacctc cattattacg ggacttatca tttcaaggta cgaggaggca
     2401 tttaccgcag ttccccttct ggtaacattc ctccccatgc ttatggatac gggcggcaac
     2461 agcggctcgc aggcatcagc ccttattatc agcagccttg ccacgggcga gctttctccc
     2521 aaggattatt tcaaggttct ttggaaagag ttcagaattt caattatttg ttcaatcgct
     2581 ctggcggtcg caaacggaat cagaatcttt gttcagtacg gcaactggca gattgccatg
     2641 gttgtatcgc tgacgcttgc gggaaccgtt gtctgcgcaa aaatcatcgg ctgcaccctg
     2701 cccatgctgg caaagcgtct gggtcttgac cccgcgctta tctcatcgcc ctttatcaca
     2761 acaatggttg acgccttctc ggtattcctc tatttcaaca tcgctatagc tattttaggc
     2821 aagctctaag ccgttaacat aacaaaatga ttggagcgca aaatggaact cagctttgta
     2881 agcccctgcc tgttaggtct ggagagcgcc gttgcctttg aactgaagga aatgggcgcc
     2941 cgcgatgtaa acgccgaaaa cggcagagtc ctctttaaag gggacga
//
LOCUS       DAONVQ010000162         2978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_22693_length_2978_cov_3.753678, whole genome shotgun sequence.
ACCESSION   DAONVQ010000162 DAONVQ010000000
VERSION     DAONVQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22693_length_2978_cov_3.753678"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /gene="argJ"
                     /locus_tag="PLD98_08495"
     CDS             <1..1106
                     /gene="argJ"
                     /locus_tag="PLD98_08495"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HQA06320.1"
                     /translation="SETEASAASIYTQNLVKGAPLIVTKEHLKDGKAKAIICNSGNAN
                     TCNDNGIEIAEGICDILAKELGIDSKDVIVASTGVIGQKLSLSPFESGIPQLVKELGE
                     NSSAAADAIMTTDLKRKEIAAEFELSGKKCRMGAIAKGSGMIHPNMATMLCFITTDAA
                     VAPDILHKALVTAANDTFNMLSVDGDTSTNDMVSILANGMADNPIISEENEDYNVFVS
                     VLTEISKEITRMLAADGEGATKLLQCEVVGAKDKESAKKAAKSVICSSLFKAAMFGAD
                     ANWGRVLCALGYSGADLDTGKIDVAFKSKNGYIEVCRNGAGVDFSEETAKEVLTADEI
                     EILINLNAGNEKATAWGCDLSYDYVKINGDYRS"
     gene            1118..1975
                     /gene="argB"
                     /locus_tag="PLD98_08500"
     CDS             1118..1975
                     /gene="argB"
                     /locus_tag="PLD98_08500"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQA06321.1"
                     /translation="MELSNAQRAEILVQALPYIQKYYNKIIVIKYGGNAMLNCSLKKS
                     VMRDIVLLSLIGIKVVLVHGGGPEISDMLSKTGKESKFIGGLRVTDEETMDIVQMVLA
                     GKINKELVSLINSIGGKAIGLSGIDGAMIEAQQLSQELGFVGEIVNIDAKIINDVLEK
                     GYIPVVSSIGYDRHGNSYNINADTAAAEIASSLKATSFILMTDTVGLLRDKNDAGTLI
                     PKVSIDEAPELIKSGIISDGMLPKVSCCINAVEKGVERVFIIDGRVPHSILIEVLTDK
                     GIGTMFYKQ"
     gene            2004..>2978
                     /locus_tag="PLD98_08505"
     CDS             2004..>2978
                     /locus_tag="PLD98_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQA06322.1"
                     /translation="MSIFDNDRKYIADTYKRFKVAFVKGKGSLLYDVDGKEYIDLASG
                     VAVNIFGICDDEWKEAVINQLNQIQHTSNMFYSLPQTELARILCERTGMKKVFFSNSG
                     AEANETAIKAVRKYSYDKYGEGRHTIITLECSFHGRTMGALSATGQPAYHVGFGPILE
                     GFVYTPANDVEKLRENVEKNNVCAIMLEFIQGEGGLNMLTEEFIKAAAEICKEKDILF
                     IADEVQTGNGRTGKLYAYMHYGLEPDVITTAKGLGAGLPIGATMLGEKLKDVFAPGTH
                     GATFGGNPVCAAGAVSVMNRLTDDLMAEVKEKGEYIKTQLKDAKGVKSV"
BASE COUNT          886 a          654 c          775 g          663 t
ORIGIN      
        1 tttcagaaac cgaagcctca gcggcatcga tatatactca gaatttagtt aagggcgctc
       61 ccctgattgt cacaaaggag catttaaagg acggaaaagc aaaggctatt atctgcaaca
      121 gcggcaacgc taatacctgc aacgacaacg gcattgaaat cgcggagggc atttgcgaca
      181 ttctcgcgaa agagctggga attgactcaa aggatgtaat agttgcctct acgggcgtta
      241 tcggtcagaa gctcagcctc tccccctttg aaagcggtat cccgcagctt gtaaaagagc
      301 tgggagaaaa cagctccgcc gcggctgacg ccattatgac cacagattta aagcgcaagg
      361 aaattgccgc tgagttcgag ctctcgggca aaaagtgcag aatgggcgct atagctaagg
      421 gctcgggcat gattcatccc aacatggcta caatgctttg ctttatcacc acagacgcgg
      481 cagttgctcc cgatattctt cacaaggctt tggttacagc cgcaaacgat acatttaata
      541 tgctgagcgt cgacggagat acctcaacaa acgacatggt gtcgatactt gcgaacggta
      601 tggcggacaa tccaatcata agcgaagaaa acgaagacta taatgttttt gtaagcgtat
      661 tgactgagat ttcaaaagag attacccgca tgctggcggc agacggtgag ggcgctacaa
      721 agctccttca gtgcgaggtt gtaggcgcaa aggataaaga aagcgcaaaa aaggccgcca
      781 agtcggtcat ttgctcaagt cttttcaagg ctgcgatgtt cggcgccgac gccaactggg
      841 gcagggtgct gtgcgctctg ggatattcgg gcgctgacct tgacacggga aagattgatg
      901 tagctttcaa gagcaaaaac ggctatatcg aagtctgcag aaacggcgcg ggcgttgact
      961 tcagcgagga aacagcaaaa gaagtgctta ccgcggacga aatagagatt cttataaacc
     1021 ttaacgccgg aaatgaaaaa gctacggctt ggggctgcga cctgagctat gattatgtaa
     1081 aaataaacgg cgattatcgc tcgtaaagag gtgcaaaatg gagctgtcta acgcgcagag
     1141 ggcggagata ctggttcagg ctctgcccta catacaaaag tactacaaca aaattatcgt
     1201 catcaaatac ggcggcaacg caatgctgaa ctgttctttg aaaaaatccg taatgaggga
     1261 tattgtcctg ctctcgttaa taggtattaa agtagtgctg gttcacggcg gcggtcccga
     1321 aatctccgac atgctttcca aaacgggcaa ggaaagcaag tttatcggcg gtctcagagt
     1381 caccgacgag gaaacaatgg acatcgttca gatggtgctt gcgggcaaaa tcaacaagga
     1441 acttgtaagc cttataaaca gcataggcgg caaagctatt ggacttagcg gcattgacgg
     1501 agccatgatt gaagctcagc aattgtccca agagctggga tttgttggag aaattgtaaa
     1561 tattgacgca aagattatta acgatgtact tgaaaaaggg tatattcctg tagtatcatc
     1621 tataggatac gaccgtcatg gaaactcata caacataaac gcggacacag ccgcagcgga
     1681 aattgcttcg agtcttaaag caaccagctt tattcttatg accgacacag ttggactctt
     1741 gagggataag aacgacgcag gtacgcttat ccccaaggtt tcaatagatg aggcaccgga
     1801 acttattaaa tcgggcatca tttctgacgg aatgctgccg aaggttagct gctgcattaa
     1861 cgctgtagaa aagggggttg aaagagtttt cattattgac ggcagagttc cccattcaat
     1921 tctgattgag gtattgaccg ataagggaat cggaacaatg ttttacaagc aatagggaac
     1981 atcttacagt aggggaagat aatatgagta tttttgacaa tgaccgcaag tacatagccg
     2041 atacctacaa aagattcaag gtagctttcg ttaagggcaa aggctcgctc ttatacgatg
     2101 tggacggaaa agaatacata gaccttgcgt cgggcgttgc ggtaaacata ttcggcattt
     2161 gcgacgatga atggaaggaa gctgttataa atcagcttaa ccaaattcag cacacatcga
     2221 atatgtttta ctctctgccc cagactgagc ttgcgcggat tctttgcgag cgcacaggaa
     2281 tgaaaaaggt tttcttttcg aactcgggtg cggaggcgaa tgaaacagca atcaaggccg
     2341 tcagaaaata ctcttatgac aaatacggcg aaggcagaca cacaataatc acacttgaat
     2401 gcagcttcca cggcaggacc atgggtgccc tttccgcaac gggacagccc gcttatcatg
     2461 tgggattcgg tcccattctg gagggctttg tatatacccc cgcaaacgat gtagagaagc
     2521 tcagagagaa tgttgagaaa aacaatgttt gcgccattat gcttgagttt attcagggcg
     2581 aaggcggact taacatgctg acagaggagt ttataaaggc tgccgccgaa atctgcaagg
     2641 aaaaggatat tcttttcatc gcggacgagg tacagaccgg caacggcaga acaggcaagc
     2701 tctacgccta catgcactac ggtcttgagc ccgatgtaat caccaccgca aagggacttg
     2761 gcgcgggtct ccccataggc gccaccatgc tgggagaaaa gctgaaggat gtattcgctc
     2821 ccggtaccca cggcgcgaca ttcggaggca accccgtttg cgcggcgggc gccgtttctg
     2881 ttatgaacag gctgaccgat gacctgatgg cagaggtcaa agaaaaggga gaatacataa
     2941 agactcagct caaagacgcg aaaggcgtaa aatccgtt
//
LOCUS       DAONVQ010000163         2967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_22803_length_2967_cov_4.905220, whole genome shotgun sequence.
ACCESSION   DAONVQ010000163 DAONVQ010000000
VERSION     DAONVQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22803_length_2967_cov_4.905220"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..235)
                     /gene="spoIIIAC"
                     /locus_tag="PLD98_08510"
     CDS             complement(41..235)
                     /gene="spoIIIAC"
                     /locus_tag="PLD98_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HQA06323.1"
                     /translation="MEVDVIFKIAAVGLIVAILNHILSRSQKEEYTMITTIAGLIIVL
                     MMLLPGIDELFKYIRSVFNI"
     gene            complement(283..792)
                     /locus_tag="PLD98_08515"
     CDS             complement(283..792)
                     /locus_tag="PLD98_08515"
                     /inference="COORDINATES: protein motif:HMM:NF021090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HQA06324.1"
                     /translation="MKYIGIAMLAITIFAGARAYALSMSKRVKELEAVSSMIKFMAAE
                     IRYLMSPIQDIIKNLAEKRELSPLTFLQVCSNRMESGEDFPSAWKNAVEEYKAAGSLK
                     DDDLRLLYSLGEEIGTSDALGQEQVCQMYSELISLKLEEARGYEAKYKEFYSALGILG
                     GLAVIIILI"
     gene            complement(716..1747)
                     /locus_tag="PLD98_08520"
     CDS             complement(716..1747)
                     /locus_tag="PLD98_08520"
                     /inference="COORDINATES: protein motif:HMM:NF040318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA06325.1"
                     /translation="MKRVEDKNAQRFDEAAAVAAPEIASALLGLPESIKADTQEIRIR
                     TGRPVMLCGLYGVCFLEKSGRVAFTTYKSAMVATKEQVAATLKKASGYSLYSHQESIA
                     KGFITFGSGHRMGICGTAVGTDGRISNVRDASSLNIRIARDHTGCSVRILDLLWNGGI
                     KSLIITGPPLCGKTTVLRDMARQLSSGYRDNYFKVSVVDERFELSNFTDDGESLGINC
                     DVLRGCKKDEGIIHVVRSMAPEIVICDEIGSLSDAKAVTEGLNSGVKFILSVHSSGKE
                     DFFKRPQVRTLLSTGEFETVVFLKNAMNPGQVEEIVKAGDITDEIYRNSHAGYNDICG
                     RKGICLEYV"
     gene            complement(1896..2780)
                     /locus_tag="PLD98_08525"
     CDS             complement(1896..2780)
                     /locus_tag="PLD98_08525"
                     /inference="COORDINATES: protein motif:HMM:NF036726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HQA06326.1"
                     /translation="MRNYVSMFCDEFKYPAEAKECFLDAFDKVSACEETAGEFMGLVE
                     AYDLGDDLLDKLTNLAEKCGVHKFTLHLLYFICLSKRLRELYEENNLPYEIYYDTVSD
                     LKWKLMECHHVEGVWGSFVANWFRRFYRLDRFALGRLQFEPSEIDFDYKKGDKELKKG
                     DLVINIHIPSAGPLHKELCLDAYKRAAEFYKDRFNGKPIAFVCNSWLLYPRHTEFLPE
                     NSNILKFMEDFDIVWSKEQPHFGDCWRIFGKAYNGDPDDLPQETSLQRAYASWLKKGN
                     TVANGYGVFFYDDLPLNK"
BASE COUNT          749 a          763 c          657 g          798 t
ORIGIN      
        1 agaacggaaa taacaagggc aacagccgca accttaaaca tcagatgtta aacacggatc
       61 ttatgtactt aaacagctca tcaatgcccg gcagcagcat cataagcacg ataataagac
      121 ccgcaatagt ggttatcatt gtatattctt ccttttgaga acgggagaga atgtggttaa
      181 gtattgccac tataagaccc actgccgcta ttttgaagat tacatctact tccataaagc
      241 cctatcacct actgatattt atttcctgtt ctttatgtac tgctatatga gtatgattat
      301 tacagcaaga ccgcccagaa tccccaaagc cgaataaaac tccttgtact tcgcctcata
      361 gccccttgcc tcctcgagct ttagagagat aagctcggaa tacatttggc agacctgctc
      421 ctgacccagc gcgtcgctgg tgccaatctc ctcacccaag gagtaaagga gccttaaatc
      481 atcgtctttc agactccctg cggctttgta ttcctcaacg gcgtttttcc aagccgaggg
      541 gaaatcctct cccgattcca tacggtttga acagacttga aggaaggtaa gcggcgaaag
      601 ctctcgtttt tccgcaaggt tcttgataat atcctgtatg ggggacataa gataccttat
      661 ttcagccgcc atgaacttta tcatagatga taccgcctca agctccttta ccctcttaga
      721 catactcaag gcatatgccc ttgcgcccgc aaatatcgtt atagccagca tggctattcc
      781 tatatatttc atcggtgata tcacctgcct ttactatctc ctcgacctga ccggggttca
      841 tggcgttttt aaggaaaacc accgtttcaa actcccccgt ggaaagaagg gtcctgactt
      901 gcggtctttt gaagaaatcc tcttttccgc ttgaatggac agagagtata aactttacgc
      961 ccgaattcag accctcggtg accgcctttg cgtcacttaa actgccgatt tcgtcgcaga
     1021 ttacgatttc cggagccatt gagcggacta catggataat accctcgtcc tttttgcagc
     1081 cacgcagaac atcgcagttg attccaaggc tctccccgtc gtcggtaaag tttgatagct
     1141 caaatctttc gtccacaacc gagaccttga agtaattatc cctgtagcct gaggatagct
     1201 gccttgccat atccctcagc acggttgtct tgccgcaaag gggagggcct gtgattatta
     1261 ggctctttat gccgccgttc catagcaagt caagaattct tacagagcaa ccggtgtggt
     1321 cccttgcgat tcggatattt aaagatgagg cgtcacggac atttgagatt ctgccgtcgg
     1381 tgccgaccgc cgttccgcag atgcccattc tgtgaccgct gccaaaggta atgaagccct
     1441 tcgcaatgct ttcctgatgg ctgtagaggg agtagccgct cgccttttta agcgttgccg
     1501 caacctgctc ctttgtggca accatagcgc tcttgtatgt ggtaaaggct accctgccgc
     1561 ttttctccag aaagcacacg ccgtagagtc cgcaaagcat cacgggtctg cctgttctga
     1621 ttcttatttc ctgcgtgtca gccttaatgc tttcgggcag tcccaaaaga gccgaggcaa
     1681 tttctggcgc tgcgacggcc gcggcctcat caaatctttg ggcgtttttg tcctccactc
     1741 ttttcaccga cctttaaatg ctctttgctt aataattctt atgacatctt gtcctttgtt
     1801 agaactaatg actaatgaag acaaaacaaa aaaaatcgcc ccgaggggcg atgggcataa
     1861 aaaaatcccc ctgaaatcaa ggggaaattt tttatttatt tgtttaacgg aaggtcatcg
     1921 tagaagaaca cgccgtagcc gtttgccact gtgttgccct ttttgagcca ggaggcgtat
     1981 gccctttgca gggaggtctc ctgcggcagg tcatcggggt cgccgttgta ggcttttccg
     2041 aaaattctcc agcagtcgcc gaaatggggc tgctccttgc tccagacaat atcaaagtcc
     2101 tccatgaact ttaagatatt ggagttttcg ggcaggaact cagtgtgtct ggggtacaaa
     2161 agccatgaat tgcaaacaaa tgcgatgggc ttgccgttaa agcggtcctt gtagaactcg
     2221 gccgcccttt tatacgcgtc aaggcaaagc tccttgtgca gcgggcctgc ggagggaata
     2281 tgtatattga ttacaaggtc acccttttta agctccttgt cgcccttttt gtagtcaaaa
     2341 tcaatttcgc tgggctcgaa ctgaaggcga ccgagagcga aacggtcaag cctgtaaaaa
     2401 cgcctgaacc aatttgccac gaaggagccc catactccct cgacatgatg gcactccatc
     2461 agcttccact ttaagtctga aactgtgtca taatatatct cataggggag attgttttct
     2521 tcgtaaagct ctctgagcct ctttgaaagg cagataaaat acagaagatg cagagtgaac
     2581 ttgtggacgc cgcatttttc cgcgagattt gtgagcttgt cgagaaggtc gtcgccgagg
     2641 tcataagcct caacaaggcc cataaactcc cctgccgttt cctcgcaggc gctcaccttg
     2701 tcaaaggcgt caagaaaaca ctccttagcc tctgcggggt atttgaattc atcgcaaaac
     2761 attgacacat aatttctcat tgtaaacact cctaataaac attctaccat tccaatttgc
     2821 gaattgtggt gcggcaaaga caagaatgac aggtcttatc cccgctgcag taagataaaa
     2881 gcattgagtt ctcccccgta aagtaaattg cgggatagca gaagcccgcc tcggggtcgt
     2941 cctcgataat gcagtaatcg ctaaagt
//
LOCUS       DAONVQ010000164         2954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_22917_length_2954_cov_4.453605, whole genome shotgun sequence.
ACCESSION   DAONVQ010000164 DAONVQ010000000
VERSION     DAONVQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22917_length_2954_cov_4.453605"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /gene="pknB"
                     /locus_tag="PLD98_08530"
     CDS             complement(<1..1339)
                     /gene="pknB"
                     /locus_tag="PLD98_08530"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HQA06327.1"
                     /translation="MENYVGKILGNRYRVLELIGVGGMATVYRAYDSIDDRTVAIKIL
                     KDEFLANEEFRRRFKNESKAIAVLSHPNIVKVYDVNFGDRLQYIVMEYVEGITLKEYI
                     ETKKQIDWREAVHFVSQILRALQHAHDKGIVHRDVKPQNIMLLQNGTIKVTDFGIARF
                     CRGDTRTLAAEGPIGSVHYISPEQARGDITDDKADIYSVGVVLYEMLTGTVPFKSDSA
                     VSVAIMQVQEEAKKPTEINPNIPKGLEQITLHAMQKNPQERYQSAAEMLLDIDEFKRN
                     PQIRFDYSYFVDNEPTKHIPVTGLPQADNTYSGGYNDSEDDIGSEEFDEEVKNKTVPV
                     LTGIMIALACVVVIGGLIAAFKTNLFGLKKVEVPYFINMTYEEVIAKYPNWKFEKSLE
                     YSTTYEEGIVFAQRPAPHEKVKANTVIILSVATRGETIQIPVLEGDQFPVAETK"
     gene            complement(1354..2082)
                     /locus_tag="PLD98_08535"
     CDS             complement(1354..2082)
                     /locus_tag="PLD98_08535"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HQA06328.1"
                     /translation="MKISAKTDRGRVRPDNQDSYAVGEMTGGVAWAVVCDGMGGAAGG
                     YLASTESVRIISERLASSYRPGMNETSVKNILVSAINAANVNIYDISSTNSEFSGMGT
                     TVVAAIVCEDTVYIAHVGDSRAYIINSQGVRQLTRDHSLVQNLLDVGKLSPNEAKEHP
                     YKNVITRALGVNETVEVDFCVESFSKDDALLICTDGLTNFVSTDDILKSTADGKVYEF
                     AERLVTAANNNGGGDNITAVVISH"
     gene            complement(2087..2932)
                     /gene="rlmN"
                     /locus_tag="PLD98_08540"
     CDS             complement(2087..2932)
                     /gene="rlmN"
                     /locus_tag="PLD98_08540"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQA06329.1"
                     /translation="MHCKSKSLKRNFIVLKYLFRLFDGQCIESVLMQYKHGYSLCVST
                     QAGCKMGCSFCASTKNGCARNLTASEILSEVYTAEKDTGVRVSNIVLMGIGEPLDNYD
                     NVIKFIRLISHPEGKNISVRNITLSTCGIVPGIYKLIEENLPITLSVSLHAPNDEKRS
                     EIMPVNKKWGVKEVVRAAKDYYEKTGRRVSFEYALVKGVNDSKSDADELSSLLKGLNC
                     HINLIPVNEIEKSGHKAPDKEHVRRFTEYLSEKGLNVTVRRTLGADINASCGQLRASA
                     IKEVE"
BASE COUNT          678 a          706 c          692 g          878 t
ORIGIN      
        1 gctttgtttc ggctacgggg aactggtcgc cctcaagaac gggaatctgt atggtctcac
       61 cccttgtggc gacggaaaga attataactg tgtttgcctt taccttttca tgaggcgcag
      121 gtctctgtgc aaagactatg ccttcttcgt aagtagttga atattcaagg gatttttcga
      181 acttccagtt cggatacttt gcaatgacct cttcataggt catattgata aagtacggaa
      241 cctcgacctt tttaagtccg aaaagattgg ttttgaaagc ggcaataaga cccccaatca
      301 caactacaca tgcaagggca atcataattc ccgtaagcac gggcacggtc ttgtttttga
      361 cctcctcgtc aaactcctcg ctgccgatgt cgtcttcgga gtcgttgtag ccgccggaat
      421 aagtgttgtc tgcctggggc agacccgtaa cgggtatatg ctttgtgggc tcgttgtcca
      481 caaagtagga atagtcgaat ctaatctgcg ggttgcgctt gaactcgtcg atatcaagaa
      541 gcatttcagc cgcggactga tagcgttcct ggggattttt ctgcatagcg tggagggtaa
      601 tctgctcaag gcccttgggg atgttggggt taatctccgt gggctttttt gcctcctcct
      661 gcacctgcat tatggcgacg gaaacagcgc tgtcggactt gaagggaact gtgcccgtga
      721 gcatttcgta aagaaccacg ccgacagagt agatatctgc cttgtcgtct gtgatgtctc
      781 cccttgcctg ctcggggctg atgtagtgga cggagccgat ggggccttca gccgcaagag
      841 tccttgtgtc gccgcggcag aaccttgcga tgccgaagtc tgttaccttt atagtgccgt
      901 tttgaaggag cattatgttc tggggcttta catccctatg aactatgccc ttgtcatggg
      961 catgctgaag ggctctgaga atctgcgaca caaaatgcac agcctctcgc cagtcgattt
     1021 gcttctttgt ttctatatat tccttcagcg ttatgccttc tacatactcc attacaatgt
     1081 actggagcct gtcgccgaaa ttaacatcgt aaacctttac aatgttcgga tgtgaaagaa
     1141 ctgcgattgc ctttgactcg tttttaaagc gtctgcggaa ctcctcgttt gcaaggaatt
     1201 catcctttag tatttttatg gcaactgtac ggtcgtctat gctgtcatag gctctgtaaa
     1261 ccgttgccat tccgcctacg ccaatcagct caagaactct gtatcggttg ccgagtatct
     1321 tgcctacata attctccata tgatacacct ctgtcaatgc gaaataacca cggcggtgat
     1381 gttgtcccct ccgccattgt tattggcagc agtcacaagc ctttcggcaa attcgtagac
     1441 cttgccgtcg gctgtggatt tgagtatgtc atcggtgctt acaaaattgg taagaccgtc
     1501 ggtacagatt aaaagcgcgt catcttttga aaaactttcg acgcaaaagt caacttctac
     1561 agtttcattc actccgagag ctctggtaat cacatttttg taaggatgct cttttgcttc
     1621 gttgggcgaa agcttgccca catcgagaag gttttgaaca agggagtggt cccttgtgag
     1681 ctggcgaact ccctgtgaat tgataatata ggctctgctg tcgcctacat gggcgatgta
     1741 aaccgtatcc tcgcaaacta tggcggcgac aacggttgtg cccattccgg aaaactcgct
     1801 gttggtggat gaaatatcat atatgttcac attcgcggcg ttgattgccg aaacgagtat
     1861 gttcttaacc gatgtttcgt tcataccggg acgatatgag gacgcaagac gctctgagat
     1921 tatgcgcacc gactcggtgc tcgccaaata tccgcccgca gctccgccca tgccgtcgca
     1981 tacgacagcc catgccacgc cgccggtcat ttcgccgaca gcataggaat cctgattatc
     2041 gggacgaacc cgccctctat cggtttttgc ggatattttc atttaatcac tccacttcct
     2101 taattgcaga tgctctgagc tgaccgcagg aagcatttat atcggcgccc agcgttcttc
     2161 tgacggttac attaagacct ttttccgaaa gatattctgt aaatctcctg acatgttcct
     2221 tgtcgggagc tttgtgacct gacttttcta tttcgttcac cggaataagg tttatatggc
     2281 agtttaagcc ctttaacagt gaagacaatt catctgcatc ggatttggaa tcgtttacgc
     2341 cttttacgag cgcgtattca aaggaaactc tgcgccccgt cttttcgtaa tagtcttttg
     2401 ccgcccgaac cacttctttt acgccccatt ttttgtttac gggcattatt tcggagcgtt
     2461 tttcgtcatt cggggcgtga agggaaacgg acagggttat gggaaggttt tcttcaatca
     2521 gcttataaat tccaggaact attccgcagg ttgaaagggt tatgttcctg acgcttatgt
     2581 tttttccctc cgggtgggag atgagcctta tgaactttat tacattatcg taattgtcaa
     2641 ggggctctcc tatgcccata agaactatgt tggaaaccct gactcctgtg tccttttccg
     2701 ctgtataaac ctcagacaaa atctcggaag ctgtaaggtt ccttgcgcag ccgttttttg
     2761 tggatgcgca gaagctgcag cccattttgc agcctgcctg agtggaaacg cacagagaat
     2821 agccgtgctt atattgcatt aagacacttt ctatgcattg tccgtcaaaa agtctaaaaa
     2881 ggtattttaa cacaataaaa ttccttttta gactttttga cttacaatgc atagtaagtg
     2941 gcttattgaa agat
//
LOCUS       DAONVQ010000165         2948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_22976_length_2948_cov_4.854131, whole genome shotgun sequence.
ACCESSION   DAONVQ010000165 DAONVQ010000000
VERSION     DAONVQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22976_length_2948_cov_4.854131"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLD98_08545"
     CDS             <1..522
                     /locus_tag="PLD98_08545"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06330.1"
                     /translation="RIDTALKSAIDKLVDEKAGIIKLFTPAFTHFGKQTGYVNAYPQG
                     VRENGGQYTHAAVWLCLAMFRNNQPDEGYRLLRLINPAAKLEDREKALRYKTEPYAFA
                     GDVYSAKGMEGRGGWSLYTGAAGWYYRTVYEELLGIKQRDGKITISPKLPSGFEGSKI
                     KLIIGGKENEFTL"
     gene            789..2675
                     /locus_tag="PLD98_08550"
     CDS             789..2675
                     /locus_tag="PLD98_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06331.1"
                     /translation="MKKTLMKLSAVLLAVCLLLPVSFVYTGGNTGTVNAPDEALAPAE
                     DEGTQTAEAPAPVVKENFFKPSGDGYVQYPTIVVPGIMQSEVVMLDENGNRKVDKNGR
                     YILSGPDADANGIFWSLIGPLFNALVFKKMDDLYKAIDEIVLLVTKNMRNDKNGQPIE
                     AREVIKYPNSFAESTPEEQRQILNTIPLHDYVENAGGESLYFFTYDSFGNQLDIAADL
                     YEFIQKVKSDRNTDKVNLCPISLGATVMNSLMDVYPDVKDSINKVIYIVPAANGSKLA
                     AGLFRAEFNRNGEDIYKNLLPSLVDGFAGFALNLVVRILPKNTLLGILDAVLRSVRDN
                     FLTTCTNLWALVPNEDYMEFRNALLMSDDMAVIREQTDRYYQAQANRFDNIMSFIDSG
                     VRFYCLVDYGVPTYNFIPNWDDHNSDGLVHLESASFGAYAAPIGKTLPEGYVQKNTSP
                     YISNPNHNYISPDNVVDASAGLLADTTWYFRNQDHEATARNDVIINLASTLMSSDRIE
                     NIFSDPKYPQFLVGRNARSLKYDLLKKALEVDTSELTDEQKAALQAAIDEVQAVLDNP
                     LADQNEISRVSAKLVNVLADIGLVGRPKEERKEDKLLYYFFKVLSDITYKMVGEKGYA
                     DLKA"
BASE COUNT          766 a          798 c          736 g          648 t
ORIGIN      
        1 agaatcgaca ccgctcttaa atccgccata gacaagcttg tcgacgaaaa agcgggaata
       61 ataaagctat ttacccccgc gtttacccac ttcggcaagc agacgggcta tgttaacgcc
      121 tacccgcagg gcgtcaggga aaacggcggt cagtacaccc acgctgccgt atggctctgc
      181 ctcgcgatgt tcaggaacaa tcagcccgat gagggttaca gactgctgcg ccttatcaac
      241 cccgccgcaa agctggagga cagggaaaag gcgttaagat acaaaaccga gccatatgcc
      301 ttcgcgggtg atgtgtactc cgcaaaaggc atggagggca gaggcggctg gagcctttac
      361 acgggcgcgg cgggctggta ctaccgcacc gtttacgagg agcttttggg aattaagcaa
      421 agggacggca aaatcaccat ttcgccgaag ctcccgagcg gctttgaagg ctccaaaata
      481 aaactcataa tcggcggcaa ggaaaacgaa tttacgcttt aaagtcaaaa tttgcatttc
      541 cccataaaaa aaatgaccat agctttaaac ggagagagcg ggctaaaacc cgctcttttc
      601 cattgttttt aatttacatt ttgtttacaa tagaatgcac atataaggcg tcttttctat
      661 ttactttttt gtaaatgctg tgctataatg ctagtgtcaa gatttatgcg ccgagcaact
      721 ttttgccgca aagtcaaaaa agcggccgct cggcttaaat acgctaattc aagcggaggt
      781 tttttggaat gaagaaaact ttaatgaagc tttcggcagt tctgcttgcg gtatgcctgc
      841 tcttgcccgt ttcattcgtt tatacgggcg gaaacacagg caccgttaac gcccccgacg
      901 aggcgctggc ccctgccgaa gacgagggaa ctcagaccgc tgaagctccc gctcccgttg
      961 tgaaggagaa tttctttaaa ccctcgggtg acggctatgt tcagtatccc acaatcgtgg
     1021 ttcccggcat catgcagtcg gaggtcgtaa tgcttgacga aaacggcaac cgcaaggttg
     1081 acaagaacgg cagatacata ctctccggac ccgacgccga cgccaacggc atcttctgga
     1141 gtcttatcgg cccgctcttc aacgctcttg tgtttaagaa gatggacgac ctttacaagg
     1201 ctattgacga gatagttctt cttgtcacaa agaacatgag gaacgacaag aacggtcagc
     1261 ccatcgaagc aagagaggtt ataaagtacc cgaacagctt cgctgagagc actcccgagg
     1321 agcagcgtca gattctcaac accatcccgc tgcacgacta tgttgaaaac gcaggcggag
     1381 agagcctcta cttctttaca tacgacagct tcggcaatca gcttgacatt gccgccgacc
     1441 tttatgagtt cattcagaag gtaaagagcg acaggaatac ggacaaggta aacctttgcc
     1501 ccataagcct tggcgctacc gttatgaaca gccttatgga tgtatatccc gatgttaagg
     1561 attcaataaa taaggttatc tacattgttc ccgccgcaaa cggctcaaag ctggccgcag
     1621 gtcttttcag agccgagttc aaccgcaacg gcgaggatat ctacaagaac ctcctgccct
     1681 cacttgttga cggctttgcg ggctttgccc ttaaccttgt tgtaaggatt cttccgaaaa
     1741 acacgcttct cggcatactt gacgccgtac tcaggagcgt aagggacaac ttccttacaa
     1801 cctgcacaaa cctttgggct cttgttccca atgaggatta catggagttc agaaacgccc
     1861 tgcttatgag cgacgacatg gcggtcataa gggagcagac agacaggtat tatcaggctc
     1921 aggcaaacag attcgacaac atcatgagct ttattgacag cggcgtaaga ttctactgcc
     1981 ttgtcgacta cggcgtaccc acctacaact tcattcccaa ctgggacgac cacaactccg
     2041 acggacttgt gcatcttgag tccgcgtcct tcggagcata cgcggctccc atcggaaaga
     2101 ccctgcctga gggctatgtc cagaagaaca caagccccta catctcgaac ccgaaccaca
     2161 actacatctc ccccgacaat gtggttgacg cttccgcggg tctgctcgct gacacaacat
     2221 ggtacttcag aaatcaggac cacgaggcaa ccgcaagaaa cgatgtcatt attaaccttg
     2281 cctccacact catgtccagc gacagaatcg aaaacatctt ctcagacccg aagtacccgc
     2341 agttcctggt tggcagaaac gcgagaagtc tgaagtatga ccttctgaag aaggctctcg
     2401 aggttgacac ttcagagctg acagacgagc agaaggcagc gctgcaggca gccatcgacg
     2461 aggtacaggc agtgcttgac aacccgctgg cagaccagaa cgagatatcg agagtcagcg
     2521 caaagcttgt caatgtcctt gccgacatcg gacttgtagg cagaccgaag gaagagagaa
     2581 aagaggataa actgctctat tacttcttca aggtcctcag cgacataacc tacaagatgg
     2641 ttggcgaaaa gggctacgca gacttaaaag cataaaacaa gccgccccac tcggggcggt
     2701 tttttaatcc ccgccgaggc gggttttatt ttttctcggt cggtcaccct catcaggcgg
     2761 ggcgtttggc tctccccact gcggggcagg gcaactctcc ctctgcgtta cggcactccc
     2821 ccttggcggc acacccctct gtgggtggcg ggcggtgctg ggtgttccct ctcgacagag
     2881 gcgttccccc tcggcagctc ggttgtttga cggcgcacca tcccgctggg ggcgggtcgg
     2941 gactcgcc
//
LOCUS       DAONVQ010000166         2903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_23453_length_2903_cov_4.866222, whole genome shotgun sequence.
ACCESSION   DAONVQ010000166 DAONVQ010000000
VERSION     DAONVQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23453_length_2903_cov_4.866222"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLD98_08555"
     CDS             complement(<1..329)
                     /locus_tag="PLD98_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQA06332.1"
                     /translation="MKKELAKQYNPREVEDRLYAFWLKGEYFKAKADKNLPTYTIVIP
                     PPNITGQLHMGHALDETLQDILIRWRRMQGYDTLWLPGTDHASIATEVKIVEKMKEEG
                     LSKEDVG"
     gene            complement(386..1108)
                     /locus_tag="PLD98_08560"
     CDS             complement(386..1108)
                     /locus_tag="PLD98_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06333.1"
                     /translation="MANCPHCNYKLRFWHIKAECPSCGVNIPNYNWEERLEQDADIAE
                     AALKKFRLKVAAIKSTLFGSAVRIVRFIMTFVPLIIFPLPFIKFALYIPFPREPKSYS
                     LINIAMGIVKEYNIKAIINTLNTTYFKVPAAVILASLILVAVAVLFGVLNFVFMLTSS
                     FSMKNTKSIFCCAVSTLAFLALLIMLLVFKANVLSAYADIIHFGLGPAIYIGLALFIT
                     NLTLNIIIHPSLKRQRKEHFSL"
     gene            complement(1188..2336)
                     /locus_tag="PLD98_08565"
     CDS             complement(1188..2336)
                     /locus_tag="PLD98_08565"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HQA06334.1"
                     /translation="MGDIYFSKDKNNKNFNIDPDSVTMELPDIDEFLEAQKEELGKNK
                     MPSTKRRENVPNFFRNASSKGKNKNTRASLALKIVAAFLALLAVAGGGASIYLYKSIL
                     GEIQYEQNEKHENEFVKNSELMSSRHVKNILLIGIDSDSTEDVARSDSMILVSIDSKN
                     KAIKLTSFLRDMWVEIPGKKTAKLNAAASSGGPTLVMDTIEYNFKIDIDNYVMIGFEA
                     FEKIIDSVGGIEVDVSQSEAKEMAKFGCVVTAGKNVHLNGEQALWFSRVRKMDSDFHR
                     TSRQRLIFEKAFAKAKKMGIPKLLEIANNVAPLISTDLDKNELLKLGANGIVKYLSYD
                     IMQGSVPVQGSWSNARISGQAVLKVDLKKNIEYLKEFIFESKPEDNKK"
     gene            2724..>2903
                     /locus_tag="PLD98_08570"
     CDS             2724..>2903
                     /locus_tag="PLD98_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06335.1"
                     /translation="MLGRYVKVSITAYLGRQDEKDKLKQTLNAGIVSIKSSDGGSYFA
                     DVYILGASNVQKEFSG"
BASE COUNT          804 a          636 c          581 g          882 t
ORIGIN      
        1 ctgccaacat cctctttgga aagaccctct tccttcatct tctctacgat tttaacctct
       61 gttgctatgg aagcgtggtc tgtgccgggc agccacaatg tgtcatagcc ctgcattctg
      121 cgccagcgga taagaatatc ctggagcgtc tcgtcaaggg catgtcccat atgaagctga
      181 cccgtaatgt tcggaggcgg aataactatg gtataagtgg gcagattctt gtctgctttt
      241 gctttaaaat attcgccttt aagccaaaaa gcatataatc tatcctcaac ctcgcgggga
      301 ttatactgct ttgccagctc ttttttcatc aatatcgtcc tttcagaaag ggagtgtata
      361 tttcgcggca atgccgctgt taaaatcaaa gtgaaaaatg ctcttttctc tgtcttttta
      421 gtgacgggtg aatgattatg ttaagtgtca aattggttat aaatagggca agacctatat
      481 aaattgcggg gcctaaaccg aaatgtatta tgtccgcata agccgaaagc acatttgcct
      541 taaataccaa gagcataata agcagcgcca agaatgccag agtgctcaca gcgcagcaga
      601 atatgctctt tgtgtttttc atcgaaaatg agcttgtaag cataaacaca aagtttaata
      661 ccccgaaaag caccgcaacc gcaaccaata tcagcgatgc cagaatcaca gccgcgggaa
      721 ccttgaagta agtggtgttt aaagtgttaa ttatagcttt gatattgtac tcttttacta
      781 ttcccattgc aatgttgatt aatgaataag acttcggctc ccgtgggaag ggtatgtaaa
      841 gcgcgaattt tataaacggc aaggggaaga taatcagcgg tacaaatgtc attatgaatc
      901 taactatcct caccgcgctg ccgaaaaggg tgcttttaat tgcggcgact ttaagcctga
      961 acttttttaa ggcggcctca gctatgtccg cgtcctgttc aaggcgttct tcccagttgt
     1021 agttgggaat attgaccccg caggagggac actcagcttt tatatgccaa aatctcagct
     1081 tgtagttgca atgcggacaa ttggccatat tgcacctctc ttattaattt agctaagaca
     1141 taatagcaca aaaattaacc atttttcaac atttgccgca acgctgatta ttttttgttg
     1201 tcttccggct tgctttcaaa gatgaattcc tttaaatact caatattctt ctttaaatcc
     1261 accttgagca cagcctgacc gcttatcctg gcgttgctcc atgagccttg aacgggaacg
     1321 ctgccctgca ttatatcata gctcaaatac ttaacaatgc cgtttgcgcc cagtttcaaa
     1381 agctcgtttt tgtcaaggtc ggtgcttata aggggagcga cattattcgc gatttcaagg
     1441 agcttcggaa ttcccatctt cttagccttg gcaaaagcct tttcaaaaat aagacgctgc
     1501 ctgcttgttc tgtgaaagtc cgagtccatc tttctgaccc ttgagaacca aagagcctgc
     1561 tcaccgttaa gatgaacatt ctttccggcg gttaccacgc agccgaactt agccatttcc
     1621 ttagcctcgc tctggctgac atccacctca atgccgccca cggagtcaat aatcttctca
     1681 aaggcctcaa agcctatcat tacatagttg tcaatgtcga ttttgaaatt gtactcaatg
     1741 gtgtccatta caagagtggg gccgccgctt gacgccgcgg cgttcagctt tgcggtcttc
     1801 ttgccgggga tttcgaccca catatccctt aaaaacgatg taagttttat agccttgttt
     1861 ttgctgtcga tgctgacgag tatcatggag tcggagcggg cgacatcctc tgtggaatcg
     1921 ctgtcaattc ctataagcag aatgttcttt acatgcctag aggacataag ctccgagttt
     1981 ttaacaaact cgttttcgtg tttttcattc tgctcatact gtatttctcc gaggattgac
     2041 ttgtaaagat aaatcgaggc gccgccgccg gcaaccgcca gcagagccaa aaaggcggcc
     2101 actattttta aagcgagaga agcacgggta ttcttattct tgccctttga cgaggcgttt
     2161 ctgaaaaagt tcgggacatt ctcacgcctt ttggttgagg gcattttatt tttacccagt
     2221 tcctcctttt gagcctccaa aaactcatct atatccggca gctccatggt tacgctgtca
     2281 gggtctatat tgaagttctt attgttcttg tcttttgaaa aatatatgtc acccatattg
     2341 cgtttccttt cccccaaaat aggatttatc agaggctgga tacagtgaat agtttaacgc
     2401 aaagcccaaa tttaaacaat gctttttata gaattttaat aaatgcttaa atcttgttat
     2461 tatttactta aatcttgtta ttatttatta aacaagccaa aaaagaccaa acatatttgt
     2521 aaactattat tctcaaaaat ccttgaaaaa ctaatatttg taccatataa tatagcctaa
     2581 tgtttttaaa gctttgcgca gcgtattaaa tttatacggt tatttccgcc aaaaaaagca
     2641 atttaaaatt attcaccttt tacaaaaatc ttttttgtga tataatactg aaggattgga
     2701 aaaaactagt ttcggagatt gtaatgcttg gtagatatgt aaaggtaagc atcactgcct
     2761 acctgggcag acaggatgaa aaggacaagt taaaacaaac tctcaatgcg ggaatagtga
     2821 gcatcaaatc ctctgacggc ggctcctatt tcgccgatgt ttatatactg ggagcaagca
     2881 atgttcaaaa agagttctcg ggc
//
LOCUS       DAONVQ010000167         2903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_23454_length_2903_cov_4.348666, whole genome shotgun sequence.
ACCESSION   DAONVQ010000167 DAONVQ010000000
VERSION     DAONVQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23454_length_2903_cov_4.348666"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PLD98_08575"
     CDS             complement(<1..1010)
                     /locus_tag="PLD98_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06336.1"
                     /translation="MAEKLKPRIYTVATAHLDTSWNWPLETTIKEYIPKTLKENFKLF
                     EKYPEYKFSFEGSYRYELIKEYYPEDFERLKEYINKGRWFVTGSCYENGDVNIPSPEA
                     LFRNILYGNNYFKNNFGKKSVDIFLPDCFGFGYALPSIAHHAGLKGFTTQKLVWSSAY
                     GIPFDIGKWYGPDGKFIYASLDAQGYTNAFVSARNSYGARYKLCENLRKYDLPFTYKF
                     HGVGDRGGAPLESSVRAVCKDIRKNHKSKIDVLSAPADQIFRDIDTLLTEKQKYSLPS
                     WRGELVSTDHGVGSYTSRAIGKRWNRRGEQLADAAERFSAAAMSLLGREYPAVKLERA
                     WK"
     gene            1144..1671
                     /locus_tag="PLD98_08580"
     CDS             1144..1671
                     /locus_tag="PLD98_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06337.1"
                     /translation="MGLKSIDKAAAEKGKGTLTLWQFVKFIFVSLLAFIVQFGTLNLL
                     YLLPPIKELFNTPFHWFVFNYGADANGLGFFIAFNISNILAQIVAFFVNREKTFNSSA
                     NIAVTLPIYIAFTLFLVCFSAWLSPFLQEKLLAAGLDGQLSANISSMACACIQFFAYF
                     PFSKILFRKPKEEKE"
     gene            complement(1821..>2903)
                     /locus_tag="PLD98_08585"
     CDS             complement(1821..>2903)
                     /locus_tag="PLD98_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06338.1"
                     /translation="RLCGVLIILSMILAFASCKKEPSAVETATEAATDAEPVFNRIDY
                     ENIARRLDLPYKAVGSLTKYGSFVYFSAAEDGNADSPYNLLIKADAETGDTEIIAKLE
                     SDTHIDYIGVNDSYLVYSVKRPPYNKTKLFLTNLKNGGVKEIYPEESDSFGKLIPRLL
                     GDSIYWLEKNQALENLSVKSVIKYGCETGARVELHSLYSDNSKKSCFYLGDGYVLWDS
                     YINGELSYFLHSIEERKTRSIKAKGDCAEAAVYAGGYIFSAEADEESGKTVDCIIDAE
                     SGEYLSDEVYIGAAESIRASGAFIYAETRYTKSFYKVEEKRILSLGRLPDSFKGEVFP
                     AENGFMILQKGEDNKSTVLILDFSQQ"
BASE COUNT          643 a          727 c          707 g          826 t
ORIGIN      
        1 cttttccaag ccctctccag ctttacagcg ggatactccc ttcccaaaag gctcatggcg
       61 gctgcggaga aacgctccgc ggcgtcggca agctgctcgc ctctgcggtt ccagcgcttg
      121 cctatcgccc ttgaagtata ggagcccacg ccgtggtcgg tggaaacaag ctcgccccgc
      181 catgatggca ggctgtattt ctgcttttcg gtaagcagag tatcgatgtc cctgaaaatt
      241 tggtcggcgg gagcggaaag gacatctatt ttggacttgt gattcttcct tatatccttg
      301 cagaccgccc ttacggagct ttccagcggg gcgccgcccc tgtcgccgac tccgtggaat
      361 ttgtatgtaa agggcaggtc gtactttctt aaattctcgc acagcttgta gcgggcgccg
      421 tagctgttcc ttgcgctgac aaaggcgttg gtgtagcctt gagcgtcaag ggaggcgtaa
      481 atgaatttgc cgtcgggccc gtaccatttt ccgatgtcaa agggtatgcc gtatgcgctg
      541 ctccagacca gcttttgagt ggtaaagccc ttcagtcccg cgtgatgggc gattgaaggc
      601 agggcatagc cgaagccgaa gcagtcgggc aggaatatgt ccacggactt tttgccgaag
      661 ttgtttttaa agtagttgtt gccgtaaaga atgttgcgga aaagcgcctc cggcgacgga
      721 atgttcacat ctccgttttc atagcatgag cctgtgacaa accatctgcc cttgtttatg
      781 tattctttaa gcctttcaaa atcctcgggg tagtactctt ttatcagctc gtagcggtag
      841 ctgccctcaa agctgaattt gtactcagga tacttttcaa agagcttaaa gttctctttt
      901 aaagttttgg gaatatactc cttaatggtg gtttcaagcg gccagttcca tgaggtgtca
      961 aggtgagcgg tggcaacggt gtaaattcga ggctttaatt tttcggccat gttttcccct
     1021 ccaacagctt gctttgtatt ataaattata aagccttgca ttggtaaaat caaacatttt
     1081 tgccttgtta tattcgtcaa gaattggtat acttgcggta gaaactgaat tgtgaggtgc
     1141 gttatgggac ttaaaagcat tgacaaggca gccgctgaga agggcaaagg cacccttact
     1201 ctctggcagt tcgtaaagtt tatttttgta agcctgcttg cctttatcgt gcagttcgga
     1261 accttaaacc ttttgtacct tctgcccccg ataaaagagc ttttcaacac gccgttccac
     1321 tggtttgtgt tcaattacgg ggcggacgcc aacggtctcg gcttttttat agcattcaac
     1381 atctccaaca ttctggcgca gattgtcgcc ttctttgtaa accgtgaaaa aaccttcaat
     1441 tcttcggcta acatagccgt aacattgccc atctacatag cctttacact gttccttgta
     1501 tgcttttccg cttggctgtc gcccttcctg caggaaaagc tccttgccgc ggggcttgac
     1561 ggtcagctct cggcgaacat ctcctcgatg gcctgcgcct gcatacagtt ctttgcctac
     1621 ttccccttca gcaaaatcct tttcagaaag ccaaaggaag agaaagaata agttttgcgg
     1681 ccttgccttt aaaacggggg cgcataagaa agtctgctct gcgcaaataa gccgttcttc
     1741 tgaaaaggcc gccgcaataa cgcctttctt tgatatggat aaagctaaaa tattaaaagc
     1801 cctgctttgg cagggctttt ttattgctgt gaaaaatcga gaatcaatac ggtgcttttg
     1861 ttatcttccc ctttttggag tatcataaat ccgttttcag cggggaaaac ctcaccttta
     1921 aagctgtcgg ggagtcttcc gagggagaga attctctttt cctccacttt gtaaaaactc
     1981 tttgtgtagc gggtctcggc gtatataaaa gcccccgatg cccgtatgct ttcggcggcg
     2041 ccgatataaa cctcgtcgct gagatattcg ccgctttctg cgtctataat gcagtcgacc
     2101 gttttgccgc tttcctcatc cgcctcggcg gagaaaatgt aaccgcccgc atagaccgcc
     2161 gcttcggcac agtcgccctt tgccttgata gagcgggttt tgcgctcctc gatagagtgg
     2221 aggaaatagg aaagctctcc gtttatgtag ctgtcccaca aaacatagcc gtcgcccaga
     2281 taaaagcagc tttttttgct gttgtcacta taaaggctat gcagctccac cctggcgccc
     2341 gtttcgcagc cgtacttaat aacgctcttt accgacaggt tttcaagagc ctgattcttc
     2401 tcaagccagt aaatgctgtc gcccagaagg cggggaatca gcttgccgaa ggagtcggac
     2461 tcctcggggt aaatctcctt gacgccgccg tttttaaggt ttgtaaggaa gagcttagtt
     2521 ttgttgtagg gcggtctttt aacgctgtaa acaagatagc tgtcgttgac tcctatgtag
     2581 tctatatgtg tgtcgctttc aagctttgcg ataatctcgg tatcgcccgt ttcggcgtcc
     2641 gcctttatga gaaggttata aggggagtcg gcatttccgt cctcggctgc gctgaaatac
     2701 acaaagctgc cgtacttggt gaggctgccc acggctttgt agggcaaatc aagccgcctt
     2761 gctatattct cataatctat tctgttaaag acaggctcgg cgtcggttgc cgcctctgtg
     2821 gcggtctcaa cggcagacgg ctcttttttg cacgacgcga aagccaaaat catgcttaga
     2881 attatcaata cgccgcacag cct
//
LOCUS       DAONVQ010000168         2823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_24293_length_2823_cov_4.399205, whole genome shotgun sequence.
ACCESSION   DAONVQ010000168 DAONVQ010000000
VERSION     DAONVQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24293_length_2823_cov_4.399205"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLD98_08590"
     CDS             <1..373
                     /locus_tag="PLD98_08590"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HQA06339.1"
                     /translation="LSYTTFEYSDFSVEKKNDGSAACVSVTNTGSRFGREVTQIYAKI
                     RDSRYKTPNFQLCGIDSVELAPGETARLNIPVSDYWLKVVTDDGKRVAPDGGITLYAG
                     GHQPDELSVRLCGTKCLEVRV"
     gene            373..1893
                     /gene="xylB"
                     /locus_tag="PLD98_08595"
     CDS             373..1893
                     /gene="xylB"
                     /locus_tag="PLD98_08595"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HQA06340.1"
                     /translation="MMYLLGIDVGTSGTKTVLFSEKGQALASYTGEYPLYQPQNGWAE
                     QDPEDWWKAAADGIRAVLSAVPEAKKEKIAIGLSGQMHGLVMLDKEERLLRRSIIWCD
                     QRTAEEVEQMKKVVGEKRLIEISANPAMTGFTAAKILWVQNHQPDIYSRCAHILLPKD
                     YIRFRLTGEFATEVSDASGMQLMDVKNRSWSSELLTAFNIDRGVLGKMYESPELTGTV
                     HETAANETGLPKGSPVAAGAGDNPAAAIGTGIVRQGRAFNTIGTSAVIYAVTDELRLD
                     MQGRIHSLCASVPGKWTLMSCTQSAGLSLRWLRDTCCGEEIEQAKRENTDPYVIMTRL
                     AEDVPVGADRLLFLPYLMGERSPHPDPLCRSAFVGLSARHGRAHLIRAVMEGVAFSQR
                     ECLDVFREMGVDVDCMTVCGGGGKSPLWRQMLADVYNCPVNTLKVDEGGALGAALLAG
                     VGAGAYKSVEEACDAVVQYNEPQNPSPNVNAYESYYQLYKKLYPTLKPIFAELASL"
     gene            2038..2517
                     /locus_tag="PLD98_08600"
     CDS             2038..2517
                     /locus_tag="PLD98_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQA06341.1"
                     /translation="MMFRPMRRFKQAVSEEECRKILREEWRAAFSVIGDEGYPYTIPI
                     NFYYEESENRIYFHGAKEGHKVSALEKCDKVCFTVWNQGYKKEGHWEWYVTSVIIFGR
                     ARLVNDRKTTEDKLRKLALKYFPTQEEAEEVLARSIDKTQIFAIDIEHMTGKLVSEK"
     gene            2596..>2823
                     /locus_tag="PLD98_08605"
     CDS             2596..>2823
                     /locus_tag="PLD98_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HQA06342.1"
                     /translation="MAEELLFTKIVDKSALTHGITIPVAYQKALFDKLGFFLRRGEAR
                     PITIQIGGYLFDDATLANEAFDEIKYPNRADI"
BASE COUNT          774 a          686 c          748 g          615 t
ORIGIN      
        1 actaagctac accacattcg agtacagcga tttcagcgtg gaaaagaaaa atgacgggtc
       61 agcggcctgt gtcagcgtta ccaatacggg cagccgtttc ggacgggaag tgacccagat
      121 ttacgccaaa attcgtgaca gccgctataa aacccccaat ttccagctct gcggcataga
      181 cagcgttgaa ctcgccccgg gcgaaacggc aagacttaat atccccgttt cggattactg
      241 gcttaaagtc gtgaccgatg atggcaaaag agtcgctccc gacggcggca taacccttta
      301 tgcgggaggt caccagcccg atgaactgag cgtccgtctt tgcggcacaa aatgcttgga
      361 agtgagggtg taataatgta tcttttgggc atcgatgttg gcacttccgg aaccaaaacc
      421 gttctgttca gtgaaaaggg acaagccctt gcaagctaca cgggggaata ccccttgtat
      481 cagccccaaa acggctgggc ggagcaggac cccgaggact ggtggaaggc tgccgcagac
      541 ggaatccgcg cggtgctaag cgccgtcccc gaggcaaaaa aggaaaagat tgcgataggg
      601 ctttcgggtc aaatgcacgg acttgtcatg ctggacaagg aagagagact cctcagacgc
      661 tccatcatct ggtgcgacca gcgcaccgcc gaggaagtcg agcagatgaa gaaagttgtg
      721 ggggaaaaaa gacttatcga aatctcagcg aatcctgcca tgacgggttt taccgcggcg
      781 aaaatactgt gggtgcaaaa tcaccagccc gatatttact cacgctgcgc ccacatcctt
      841 ctccccaagg attatataag gttcaggctg acaggggagt ttgccaccga ggtttccgac
      901 gcctctggta tgcagcttat ggatgtaaaa aaccgctcat ggtcctcaga gctacttact
      961 gcctttaata ttgaccgcgg cgttctgggc aagatgtatg agtctcccga gcttacgggc
     1021 accgtccatg agacagccgc aaatgaaaca ggattgccca aaggctcccc cgtcgcagcg
     1081 ggcgctggcg acaatcccgc cgcggccatc ggaacgggca ttgtgcggca gggcagagct
     1141 ttcaatacca tcggcacttc cgccgttatc tatgctgtta cagatgagct gcgtcttgat
     1201 atgcagggca gaattcacag cctttgcgcc tccgtcccgg gcaaatggac ccttatgagc
     1261 tgcacccaaa gcgcgggact gtccctcaga tggctgagag acacctgctg cggcgaggaa
     1321 atcgaacagg caaagcggga aaacaccgac ccctatgtca taatgacaag acttgcggag
     1381 gatgtgccag tcggcgcaga cagacttttg ttcctgccat atctcatggg cgagcgctcc
     1441 ccgcaccccg acccgctgtg ccgcagcgct tttgtcggtc tttccgcccg tcacggcaga
     1501 gcacatttaa taagagcggt catggaggga gttgcgttct ctcagcgtga atgccttgat
     1561 gtgttccgag agatgggcgt tgatgtggac tgcatgacgg tttgcggcgg cggaggcaaa
     1621 agcccgcttt ggaggcagat gctcgccgat gtatataact gccccgtaaa caccttaaag
     1681 gtggacgaag gcggcgcatt gggagcggcg ctccttgcgg gcgtcggagc gggcgcgtat
     1741 aagagcgtgg aagaagcctg cgacgctgtg gtgcagtata acgagcccca aaatccttcc
     1801 ccaaatgtca acgcctacga gtcctattat cagctttaca aaaaactcta ccccacacta
     1861 aaacccatat ttgccgaact cgcaagtcta taaaaaccta tactgaataa aagaaccgca
     1921 ctatgctgtg cggctctttt tgtgctgaat ttttccctct aagtcggcgg gactgttgta
     1981 aaggtcgaaa aggtgtagta tgctaaaggc ataaacaggt ttcgggcggg aggaaaaatg
     2041 atgttcagac cgatgagaag atttaagcag gcggtttctg aggaagaatg caggaaaatc
     2101 ctcagagaag aatggagagc ggcgttttcg gttatcggtg atgaagggta tccgtacact
     2161 attccgataa atttctatta tgaagaaagc gaaaaccgta tttatttcca cggcgccaag
     2221 gaagggcata aggtttcagc tttagagaaa tgcgataagg tctgctttac cgtatggaat
     2281 cagggctata aaaaagaggg ccattgggaa tggtatgtca ccagcgttat aatttttgga
     2341 agggcaagac ttgtaaatga caggaaaaca accgaggaca aacttagaaa acttgcgctg
     2401 aaatatttcc cgacacagga agaagccgaa gaagtccttg cccgctcaat agacaaaact
     2461 caaatcttcg ccatcgacat cgagcacatg accggcaaac tggtcagcga aaagtaaatt
     2521 aaatttgtag tttcttattt ctccaacata aagcagcaca aaacaaaaat agtttgcaat
     2581 aaggcaggta tccgtatggc tgaagaattg ttatttacca aaatagtgga caagtcagca
     2641 ttaacacatg gaatcactat acctgttgct tatcagaaag ccctttttga taaacttggc
     2701 tttttcctca ggagaggcga ggcgcgtcca ataaccatcc agataggggg atacttattc
     2761 gacgacgcaa cacttgcaaa tgaagctttc gatgaaatta agtatcctaa tcgggctgat
     2821 att
//
LOCUS       DAONVQ010000169         2810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_24435_length_2810_cov_4.155717, whole genome shotgun sequence.
ACCESSION   DAONVQ010000169 DAONVQ010000000
VERSION     DAONVQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24435_length_2810_cov_4.155717"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PLD98_08610"
     CDS             <1..992
                     /locus_tag="PLD98_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HQA06343.1"
                     /translation="GYKKKGYKVWCVGDDIAWIRKGADGRLYAINPENGFFGVAPGTN
                     EKSNYNALASTRRGTIFTNVVHNLDNNTVWWEGLDDNPPKNALDWLGNPWDGTTSDKK
                     GAHPNSRFTAPAQNCPALSPEFENPNGVPISAFVFGGRRAKLAPLVYQAKDWNTGVFV
                     GSAMASETTAAAMGAVGVVRRDPMAMLPFCGYNMADYFRHWIEIGKTLDPDKAPKIFN
                     VNWFRKDDEGNFLWPGFGENLRVLDWIIDRCEGKVDAVETPIGYVPYAKDINLEGIED
                     EVSYETLEEILKVDKALWREEAKAIEEFYMQFGDRLPKELWDALNNLKAAVAE"
     gene            complement(1048..>2810)
                     /locus_tag="PLD98_08615"
     CDS             complement(1048..>2810)
                     /locus_tag="PLD98_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HQA06344.1"
                     /translation="KRIVALDLTGMVAGTKYRGDFEERIKNAIDEVVNSKNVILFIDE
                     LHTLVGAGSAEGAVDAANILKPSLARGEMQVIGATTLDEYRKHIEKDAALERRFQPVM
                     VGEPSEEETVEILKGIRDKFEAHHKVKITDEAIDAAVKLSVRYIGDRFLPDKAIDLID
                     EAASKVRLRAHTAPTDIQELEQRIKTISEEKAAAVNAQDFERAAALRDEERELTEKLK
                     AEKAKWQERRVDASGEVTPEEIAQIVASWTGVPVQQLTTEESERLLKLEEELHKRIVG
                     QEQAVSAVARAIRRGRVGLKDPKRPIGSFIFLGPTGVGKTELSKALAATIFGDENAMI
                     RLDMSEYMEKHTVSRLVGSPPGYVGYDEGGQLTEKIRRRPYSVVLFDEIEKAHPDVFN
                     MLLQILDDGILTDAQGRTVSFKNAVIIMTSNVGARLITDRKGSLGFAYDNSSMSEEKI
                     RDAVMGELKNTFRPEFLNRVDDIIVFSRLTKENIKEIAAKMLDKVKGIAEEIGVKVSF
                     SPEVIEKIAEAGFDEVYGARPLKRTIQSKIEDRLSELILEGAVKANNEYECRYKDGEY
                     VFEKVGGEEKKEIHTVDSDD"
BASE COUNT          591 a          780 c          720 g          719 t
ORIGIN      
        1 aaggatataa gaagaagggc tacaaggttt ggtgcgttgg cgacgacatc gcttggataa
       61 gaaagggcgc agacggcaga ctctatgcca tcaaccccga aaacggcttc ttcggcgtcg
      121 ctcccggcac aaacgaaaag tcaaactaca acgctctcgc ttccacaagg agaggcacaa
      181 tctttacaaa tgttgttcat aacctggaca acaacaccgt ttggtgggaa ggtcttgacg
      241 acaacccgcc caagaacgcg ttagactggc tgggcaaccc ctgggacggc acaacctccg
      301 acaagaaggg cgctcatccc aacagccgct ttacggctcc cgctcagaac tgccccgcac
      361 tcagccccga gtttgagaat cccaacggcg ttccgatttc cgcatttgta ttcggcggcc
      421 gcagagctaa gctcgctccg ctggtatatc aggcaaaaga ctggaacacc ggcgtattcg
      481 taggctcggc aatggcatcc gaaacaacag ccgcagctat gggcgcggtc ggcgttgtaa
      541 ggcgcgaccc gatggcaatg ctgcccttct gcggatacaa tatggccgat tacttcaggc
      601 attggattga aatcggcaag acccttgacc ccgacaaggc acccaagatt ttcaatgtaa
      661 actggttccg caaggacgac gagggcaact tcctctggcc gggattcggc gagaacctgc
      721 gcgttcttga ctggatcatc gaccgctgcg agggcaaggt tgacgccgtt gaaacaccca
      781 tcggctatgt tccctacgct aaggacatca acctggaagg cattgaggac gaggtatcct
      841 atgagaccct tgaggaaatc cttaaggttg acaaggctct ttggagagaa gaggctaagg
      901 caatcgagga gttctatatg cagtttggcg acagactgcc caaggagctc tgggacgctc
      961 ttaacaacct caaggctgct gttgcggagt aataacaaaa aaatagaagg agcggcaaaa
     1021 ccgctccttt tttattgctc tttttactta gtcgtcgctg tcgacggtat ggatttcttt
     1081 cttttcttcg ccgcccacct tttcaaagac atactcgccg tccttgtagc ggcactcgta
     1141 ctcgttgttc gccttgaccg ctccctcgag aatcagctct gagagcctat cctcaatctt
     1201 ggactgaatt gtcctcttga gcggacgggc gccgtagacc tcgtcaaagc ctgcctcggc
     1261 gattttctca attacctcgg gtgagaagct gactttcacg ccgatttcct cggcgattcc
     1321 ctttaccttg tcgagcatct ttgcggcgat ttctttgatg ttttcctttg tgaggcggga
     1381 gaatacgatg atgtcgtcga ctctgtttaa aaattcgggg cggaatgtgt ttttaagctc
     1441 gcccattaca gcgtctctaa tcttctcctc gctcatgctg ctgttgtcat acgcaaagcc
     1501 cagactgccc tttctgtcgg tgatgagcct tgcgcccaca ttcgatgtca tgatgatgac
     1561 agcgttctta aagctgactg ttctgccctg agcgtcggtg agtatgccgt cgtcaaggat
     1621 ttgcagcagc atattgaata catcggggtg agccttttct atctcgtcaa agagcacgac
     1681 ggagtagggt ctgcgcctga ttttttcggt gagctgaccg ccctcgtcat agcccacata
     1741 tccgggaggc gaaccgacaa ggcgggatac tgtgtgcttt tccatatact ccgacatatc
     1801 aaggcggatc atggcgtttt cgtcgccgaa aatggtcgcg gcaagagcct ttgaaagctc
     1861 tgttttacca acgcccgtag gaccgaggaa gatgaatgag cctatgggac gcttcgggtc
     1921 cttaagacct accctgccgc gcctgattgc ccttgcgaca gcggaaaccg cctgctcctg
     1981 acccacaatg cgcttgtgga gctcttcctc cagctttaag agtctttcgc tttcttcagt
     2041 cgtcagctgc tgtaccggaa cacctgtcca ggaggcaacg atttgagcta tttcttccgg
     2101 agttacttcg cctgaggcgt ctactcttct ctcctgccat tttgcctttt cagccttgag
     2161 cttttcggta agctccctct cctcatctct gagagctgcc gcgcgctcaa agtcctgagc
     2221 gttgaccgct gccgcctttt cttcgcttat cgtcttgatt ctctgctcaa gctcctgaat
     2281 gtctgtgggg gcggtatggg cgcggagcct tacctttgag gctgcctcgt cgataaggtc
     2341 aattgccttg tcgggcagga atctgtcgcc gatatatctg accgagagtt tgactgccgc
     2401 gtcgattgcc tcgtctgtga tttttacctt gtggtgagcc tcgaacttat ctctgatacc
     2461 cttgaggatt tcaacggtct cctcctctga gggctcgccc accattacgg gctggaacct
     2521 gcgctctaaa gccgcgtctt tttcgatgtg ctttctgtac tcgtcaaggg ttgtggcgcc
     2581 gataacctgc atctctcccc ttgccaaaga gggtttgaga atattcgcgg cgtcgacggc
     2641 gccctcagcg gagcccgctc ccacaagggt gtggagctca tctataaaga ggattacatt
     2701 cttggagtta acaacctcgt caatggcgtt tttgattctc tcctcaaagt cgcccctgta
     2761 ctttgtgccg gcgaccatgc ctgtaaggtc aagcgcgacg attcgcttat
//
LOCUS       DAONVQ010000170         2776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_24810_length_2776_cov_5.116501, whole genome shotgun sequence.
ACCESSION   DAONVQ010000170 DAONVQ010000000
VERSION     DAONVQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24810_length_2776_cov_5.116501"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..429)
                     /locus_tag="PLD98_08620"
     CDS             complement(145..429)
                     /locus_tag="PLD98_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HQA06345.1"
                     /translation="MRVKKQPIRRCTGCGESKPKRELVRVVRSPEGEISIDKTGKKNG
                     RGAYICPNPECLKKAKKAKRLDRSFEMQVPDEIYEQLEKELSTDGDAQQG"
     gene            complement(487..1545)
                     /gene="nusA"
                     /locus_tag="PLD98_08625"
     CDS             complement(487..1545)
                     /gene="nusA"
                     /locus_tag="PLD98_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HQA06346.1"
                     /translation="MNKDFFDAVRLLVKEKGLPAEHIYEKIRNAIIVAASHDYGKDAV
                     FCDINPDENEIRVYVRKNVVSEVENPQTEIHVDEAQKYKKGALPGDIVEIPLETKEFG
                     RIAAQKVKHVLRQGIKEVEQDKVYEEFQSKNQEIVTARVLVVDPRTGNVTLEIGRAEA
                     ILPKGEQVPGEVFTPGQLTRVYIVDVHNTERGPKAMISRTHPGLVKRLFEAEVPEIYD
                     GIVEIKGIAREAGSRTKIAVYSKDENVDASGACIGTRGARVGKIVELLGGEKIDIVKY
                     SDDPREYIANALAPAEIINVEIEDADQKICHVTVPDNQLSLAIGNKGQNARLAAKLTG
                     WKIDIKPESGFYSPVIQL"
     gene            complement(1579..2058)
                     /gene="rimP"
                     /locus_tag="PLD98_08630"
     CDS             complement(1579..2058)
                     /gene="rimP"
                     /locus_tag="PLD98_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HQA06347.1"
                     /translation="MSDKLKGPARVEAIRNLAAPIAESLGLKLWDVKIFKEGGRQILR
                     IVIDKDGGVSMDDCVAMHRAIDAPLDELDPIEESYNLQISSPGLEREIERPEHFEEYI
                     GENVKFKLRAPIDGKKEFSGVLKDYNEGLVIIEVDGEDMSLEKKDYAWIKLDDFPEF"
     gene            complement(2308..>2776)
                     /locus_tag="PLD98_08635"
     CDS             complement(2308..>2776)
                     /locus_tag="PLD98_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HQA06348.1"
                     /translation="PSASSSDELYFERFAILSEKAAQMGVICAQENVVHYRSQYPDFM
                     QRMKKAIGENFRMVLDIKQARRTGVDPFLFFDAVGESIEHIHISDFNDKKDCITPLKG
                     KFDFKEFFIETIKRGYKGDYIIELYRDSYKDAAEIEEAFLKLQKIMGEIKEGL"
BASE COUNT          630 a          714 c          580 g          852 t
ORIGIN      
        1 agtctttcca tttctctttg gagtctctcg gatgcgtcgc aggacagtat aataagacgg
       61 gctttgccct gctttattga tgcctttacc gcatcgtgcc ctgattggag cttgccggcc
      121 tttctgcaaa gtcccaaaaa tgatttaacc ctgctgtgcg tctccatcag ttgacagctc
      181 cttttcaagc tgctcgtaaa tctcatcggg aacctgcatt tcaaaagacc tgtcaagccg
      241 ctttgccttt ttcgcttttt tcaggcactc gggattgggg cagatgtatg cgcctcggcc
      301 gttctttttg cccgtcttgt cgatggatat ttcgccttcg ggcgacctta ccaccctgac
      361 aagctctctt ttaggctttg actctccgca gcccgtacat cttctgatag gctgtttttt
      421 aacccgcacg agactactcc tttcaggata atttatttcg cgtttagaaa aatgcaatca
      481 cggtaattac agttgaatta caggcgaata aaagccgctt tcgggcttta tgtcaatctt
      541 ccagcctgtg agctttgcgg caagtcgggc gttctgaccc ttgttgccga tggcgaggga
      601 cagctgattg tcgggaacgg ttacatgaca aatcttctgg tcagcgtcct caatttcaac
      661 atttataatc tcagcgggag cgagagcgtt tgctatatac tccctcgggt cgtcgctgta
      721 ctttactatg tcaatctttt ctccgccaag aagctcaaca atcttgccga ctctcgcgcc
      781 ccttgtgccg atgcacgcgc cggacgcgtc aacgttctcg tccttgctgt aaacggcgat
      841 tttggttctg gagcccgcct ctctcgcgat tcctttaatc tcgactatgc cgtcgtaaat
      901 ctcgggaacc tcagcctcga aaagacgctt tacaagaccg gggtgggtcc tggaaatcat
      961 ggccttgggg cctctttcgg tattgtggac atcgacgatg taaacccttg tgagctgtcc
     1021 gggggtgaat acctcgccgg ggacctgctc gcctttgggc agaatggcct cggctctgcc
     1081 tatctcaagg gtgacattgc cggtgcgggg gtccacaaca agaacccttg cggttactat
     1141 ctcctgattt ttgctctgga actcctcgta aaccttatcc tgctcaactt cctttatgcc
     1201 ctgccttaaa acatgcttaa ccttttgagc ggcgattctg ccgaactcct ttgtttcaag
     1261 gggaatttcg actatatcgc cgggcagagc gccctttttg tacttctgag cctcatcaac
     1321 atgaatttcg gtttgcgggt tctccacctc ggaaaccaca ttctttctga catagactct
     1381 tatctcgttt tcgtcgggat ttatatcgca gaaaaccgcg tccttgccgt agtcatggct
     1441 tgcggcaaca atgatggcat tgcgaatttt ctcataaata tgctctgcgg gaagtccttt
     1501 ttccttaacc agcaatctca ctgcgtcgaa aaaatcctta ttcatttatt acggtcaacc
     1561 ctttcatata tattggaact aaaattcggg aaaatcgtca agtttgatcc aggcatagtc
     1621 ttttttctca agactcatgt cctcgccgtc tacttcaata ataacaaggc cttcgttgta
     1681 gtccttcaaa acgcccgaga attccttctt gccgtcaatg ggggctctga gcttgaattt
     1741 gacattctcg ccgatatact cctcaaagtg ctctggacgc tcaatctccc tttcaaggcc
     1801 gggggatgaa atctgaaggt tgtagctttc ttctatgggg tcaagttcgt caaggggagc
     1861 gtcgatagct ctgtgcattg ccacacagtc gtccatgctc accccgccgt ccttatcgat
     1921 gacgattctt aatatctgcc tgccgccttc tttaaaaatt tttacatccc acagctttag
     1981 cccaaggctt tccgctatgg gagcggcaag gtttcttata gcctcgaccc ttgcgggtcc
     2041 ctttaattta tcagacactt taattctcct ttatttctaa ttcttttttt atagtcttaa
     2101 ccttttaagc ccgtttaaat aatcgcaaac tttccatata aaggaacggc aagactatat
     2161 ggagaactta aaacttaata ttctaaaaaa agcctaaata aagagcgggt cgccccactc
     2221 aaaagtaaag tcgctgctga tttatactat cataccacat aaaggaaaat aaagcaagta
     2281 tttttatttt gagggggact gagcgggtta taagccttct ttaatttccc ccatgatttt
     2341 ttgaagcttt aaaaacgctt cttcaatttc cgcagcgtct ttatagctgt ccctatacag
     2401 ctctattata taatctccct tgtagcccct ttttattgtt tcaataaaaa attctttaaa
     2461 gtcaaacttg cctttaaggg gagttatgca gtcctttttg tcattaaagt cgcttatgtg
     2521 aatatgctcg atgctttcgc ccactgcgtc aaagaatagg aatgggtcta cccccgtgcg
     2581 ccttgcctgc ttaatgtcaa gaaccatcct gaagttttcg ccaatagcct ttttcattct
     2641 ttgcataaaa tcggggtact gggagcggta atgcaccaca ttctcctgag cgcagataac
     2701 tcccatttga gccgcctttt cggagagaat cgcaaaccgc tcaaaataca gctcatcgct
     2761 ggaagacgct gagggc
//
LOCUS       DAONVQ010000171         2766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_24919_length_2766_cov_4.513464, whole genome shotgun sequence.
ACCESSION   DAONVQ010000171 DAONVQ010000000
VERSION     DAONVQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24919_length_2766_cov_4.513464"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PLD98_08640"
     CDS             complement(<1..939)
                     /locus_tag="PLD98_08640"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQA06349.1"
                     /translation="MAEFKHKKSEIVAVLNPSENNPRNSEGDFIELKDGRILFAYSRY
                     TGSRSEDDRPCDIACIYSHDGGKSFDMAPRILKKASEHNVENIMSVSLLRLQNGDLGL
                     FYLIKHKDALTSDYVLCRSADEGETFYSAVSCFSPHNPEYYVVNNNRVLRTKSGRLIV
                     PAALHRRHPKNIYGIDPALAVFSYSDDDGFTWKSFSGTLACPVYRSETGLQEPGVIEL
                     NNGVLYAYFRTDLMCQYESFSLDGGKSWTLPQPSAFTSPVSPLKIAQNPYTGLYYAVW
                     NPIPNYQGRDKNLHENTSLSWGRTPLVIAESEDGINF"
     gene            1137..1212
                     /locus_tag="PLD98_08645"
     tRNA            1137..1212
                     /locus_tag="PLD98_08645"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Val,seq:gac)
     gene            complement(1397..2335)
                     /gene="fba"
                     /locus_tag="PLD98_08650"
     CDS             complement(1397..2335)
                     /gene="fba"
                     /locus_tag="PLD98_08650"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HQA06350.1"
                     /translation="MPLVSTKEMLKKAYEGGYAVGAFNVNNMEIVQGITEAAKELNAP
                     LILQVSAGARKYANHTYLTKLVEAAVIETGLPIALHLDHGADFEICKSCIDGGFTSVM
                     IDGSHHDYEDNVALTKKVVEYAHAHGVTVEGELGQLAGIEDDVNVSADDAHFTDPGQV
                     EDFVTRTGVDSLAIAIGTSHGAYKFKPGQKPQLRFDILDEVARRLPGFPIVLHGASSV
                     VPEFVEKINKYGGQMPDAIGIPEDMLREAARNAVCKINIDSDLRLAMTAAIREYMALH
                     PDHFDPRQYLAPARDAIKAMVAHKIKNVLGCDGKAY"
     gene            complement(2526..>2766)
                     /locus_tag="PLD98_08655"
     CDS             complement(2526..>2766)
                     /locus_tag="PLD98_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06351.1"
                     /translation="LEAAVLNAEQAIEQTNAYTAPNTEKAQERLEKALVAAGALKPSK
                     QNDTANGLITALLKLANDAIMQYYGARGFSDHKPF"
BASE COUNT          669 a          634 c          704 g          759 t
ORIGIN      
        1 aaagtttatg ccgtcctcgc tctcggcaat aaccaagggg gtgcgtcccc agctcagaga
       61 tgtgttttcg tgaaggtttt tatccctgcc ctgatagttg gggattgggt tccataccgc
      121 ataatacaga ccagtatagg ggttctgagc gatttttaag ggagaaacgg gagaagtaaa
      181 ggcagagggc tggggaagcg tccaggactt tccgccgtca agggaaaagc tctcgtactg
      241 gcacataagg tcggttctga aataagcgta aagaacgccg ttatttagct caatcacgcc
      301 cggctcctga agtccggttt ccgagcgata aacgggacac gccaaagttc cagaaaagct
      361 cttccatgta aagccgtcat catctgaata agaaaacaca gccagagcgg ggtcgatgcc
      421 gtagatattt ttcgggtgcc gcctgtgaag tgccgcgggg actataagcc tgccgctttt
      481 ggttctgagg actctgttgt tgtttaccac atagtactcg gggttgtggg gcgaaaagca
      541 gctgacggca gagtaaaaag tctcgccctc atcggcggag cggcataaaa catagtcgga
      601 ggtaagggcg tccttgtgct ttataagata aaaaagcccc aaatcgccgt tttgcagcct
      661 taaaaggctc acgctcataa tgttctccac attatgctcc gacgcttttt ttaagatacg
      721 aggcgccatg tcaaagctct tgccgccgtc atgagaataa atgcaggcta tgtcgcaggg
      781 acggtcgtct tcgctgcggc tgcccgtgta gcggctgtag gcaaagagga ttcgcccgtc
      841 tttcagctct ataaagtcgc cctcgctgtt tctggggtta ttctcagaag ggtttaaaac
      901 agcgacgatt tcggattttt tatgtttaaa ctctgccaca tccacacccc ttttacaaag
      961 gaggaaaacc tccctacgac tattgatact accataaggc gctgcgctca gcaatagaaa
     1021 tttgcgcaaa attagttttc ttttgcgaat agggtaaaat ttcaggacaa gaaagttaaa
     1081 aaggctattg atttttttgc gactatagtt tataatacac ttcgcacagg tgatacggac
     1141 gcttagctca gttggtagag cattcgcttg acgtgcgaag ggtcagcggt tcgagcccgt
     1201 tagcgtccac cattaataag acctcgaaac cttaacacac aaaggtttcg aggtttttgc
     1261 tttttaattt tatttgcatc tgaagctcga aaacagacaa aatggattag tattgaaaat
     1321 tgcaaatcaa aacatttttg gagttaaata aaaacacccc ggcgacgctt tggagtgccg
     1381 ggtgtttttc tattatttaa tatgccttgc cgtcgcagcc gaggacattc ttgattttgt
     1441 gggcgaccat tgccttgatg gcgtcgcggg cgggggcaag atactgtctc gggtcgaagt
     1501 ggtcgggatg gagagccata tactctctga tagctgcggt cattgcgagt ctgaggtcgg
     1561 agtcgatgtt gattttgcat acagcgtttc ttgcggcttc tctgagcata tcctcgggaa
     1621 tgccgattgc gtcgggcatt tggccgccgt acttattaat cttctcaaca aactcgggaa
     1681 caacagagct tgcgccgtgg agaactatcg ggaatccggg cagccttctt gcgacctcgt
     1741 caagaatgtc gaatctgagc tggggcttct gtccgggctt gaacttatat gcgccgtggc
     1801 ttgtgccgat ggcgattgcc agagagtcaa cgcctgtgcg ggttacgaag tcctcaacct
     1861 gaccggggtc cgtaaagtgg gcgtcgtccg cggagacatt aacatcgtcc tcgatgccag
     1921 caagctgacc cagctcgccc tcaacggtaa ctccgtgggc gtgggcatat tcaacgacct
     1981 tctttgtaag ggcgacattg tcctcatagt cgtgatgtga gccgtctatc ataacggagg
     2041 taaaaccgcc gtctatgcag cttttgcaaa tctcaaaatc tgcgccgtgg tcaaggtgca
     2101 aagctattgg aagtccggtt tcgataacag cggcttctac aagctttgta aggtaagtat
     2161 ggttcgcata ctttcttgcg cctgcggata cctgaagaat cagcggagca ttgagctctt
     2221 ttgccgcttc ggtaatgccc tgcacaattt ccatgttgtt aacattgaac gcgccaacgg
     2281 catatccgcc ttcataggct tttttcagca tttcctttgt tgaaacaagt ggcatattat
     2341 acactcctaa aaataaaatt acttcttaat actaacagtt tattatggct tttaaaagcg
     2401 aatatatgag ctttttacgg cgaaaaaact catcagacaa atattatata ttaccttttt
     2461 gcaaaagtca aagatttaac gaataaaaaa cacgcggctt ttcgccgcgt gccgttttta
     2521 tattgttaga agggtttgtg gtccgagaag ccccttgcgc cgtagtactg cattatggcg
     2581 tcgtttgcca gtttcaagag ggctgtgatt aagccgtttg cagtgtcatt ctgctttgag
     2641 ggcttgagtg cgcctgccgc gacaagtgct ttctccagtc tctcctgagc tttctcggta
     2701 ttgggcgctg tgtaggcgtt tgtctgctcg attgcctgct ctgcgttcag aacggcggct
     2761 tcaagc
//
LOCUS       DAONVQ010000172         2635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_26453_length_2635_cov_3.950000, whole genome shotgun sequence.
ACCESSION   DAONVQ010000172 DAONVQ010000000
VERSION     DAONVQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26453_length_2635_cov_3.950000"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="PLD98_08660"
     CDS             <1..1386
                     /locus_tag="PLD98_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HQA06352.1"
                     /translation="YNGKTGWISLEYTIFRGDTLPHTLKRKVIDVSYHQGNINWNAVK
                     ADGVEAAILRIGVRYTDSRKIGMDTKFLEYYEGATKAGIPVGVYFYSVSLNVQQALDE
                     ANFVIAALKNNNIVLEYPVFYDIEDKNIYYLGKEMWTAMSNAFMTKIEEAGYYAGIYT
                     SKWHFETYIDPSILVGRAVWVAEYTTKENTTYKGDYGMWQYSNTGRVNGINGNVDMNW
                     CYVDWPSFIKKNGYNGFEKQDENTYWDIISAPTHEKDGERILIDKKTGKAIKGELIPR
                     LKYRASEPTVVKQPTAAQDGLMQTVCLDTGVILGQTPVPSLNSGLASYDFGTDEDWIV
                     QIAPTCETAGRRIRYAKNSGGKEVIQWELIAPIPHEAASEKTVGKNGNTEARNQTVCK
                     HCGAVIEYTPHLLYDFDLNSKVDAADARIVLRIAAKLDKATESHLIASGGDKINPNLS
                     RTILRRAAKLD"
     gene            complement(1431..>2635)
                     /locus_tag="PLD98_08665"
     CDS             complement(1431..>2635)
                     /locus_tag="PLD98_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQA06353.1"
                     /translation="SFFQNVTDVIVEPSELYLSVGEERTLTVKILPEEAGNKNVYWIS
                     GNSAVVSVNQKGEIKALSIGTAVVTAVAEDGGITGSAVITVKAAPVPVSGVTLSKNSL
                     ELMIDENFQLYYEVLPHNADNKSVRFESSNALVATVSQSGLVTGKTAGTAVITVVTMD
                     GGYTASCTVTVKPKPIKVTGVSLNKNAVILKTGKTETLFATVKPQNADNKKIIWTSSN
                     NSVATVSQNGVVTALKSGTATIKAITEDGGYTASCTVVVEENAKLKLKADSGYKLDSK
                     LGIVYNIRPNTKVSDLLKSFEAGKIEVKNSKGKLLSGSEIVPTGSKISLIGNDGVSDT
                     LLLSVLGDVNGDGQAAAADARLVLRFVAKLDSPTEYQKLSADVNGKGISAADARAILR
                     AAAKLDTL"
BASE COUNT          713 a          685 c          611 g          626 t
ORIGIN      
        1 tacaacggaa aaacaggctg gataagcctt gaatacacga ttttcagggg agataccctt
       61 ccccacacct taaagcgcaa ggttatagat gtgtcctatc atcagggcaa catcaactgg
      121 aacgcagtaa aagccgacgg cgttgaggcc gcgattctca gaatcggcgt cagatacacc
      181 gacagcagaa aaataggcat ggacacaaag ttccttgagt actacgaagg agccaccaag
      241 gcgggcatcc ccgtcggcgt gtacttctac agcgtctccc ttaatgttca gcaagctctt
      301 gacgaggcaa actttgtaat cgctgctcta aagaataaca atatagtcct cgaatacccc
      361 gttttctacg acatagagga caagaacatc tactacctcg gcaaagaaat gtggaccgcg
      421 atgtcaaacg cctttatgac aaaaattgaa gaagcgggct actacgccgg aatctacacc
      481 agcaaatggc actttgaaac atacattgac ccgtcaatac tggttggacg agccgtatgg
      541 gtcgccgagt atacaacaaa agagaacact acatacaaag gcgactacgg aatgtggcag
      601 tactccaaca caggcagggt aaacggcatt aacggaaatg tggacatgaa ctggtgctat
      661 gtggactggc cgagtttcat taagaaaaac ggctacaacg gctttgaaaa gcaggatgag
      721 aacacctatt gggacatcat ctccgccccg actcacgaaa aagacggcga gagaatctta
      781 atagacaaga aaaccggaaa agcaattaaa ggcgagctta tcccaaggct taaatacaga
      841 gccagcgagc cgactgttgt aaagcagccc actgccgctc aggacggtct tatgcagaca
      901 gtctgccttg ataccggcgt aattctcggt cagactcccg taccgtcgct taattcaggt
      961 cttgcatcct acgacttcgg caccgatgag gactggatag tgcagattgc ccccacctgc
     1021 gaaaccgcgg gcaggagaat caggtacgca aagaactcag gcggcaaaga ggtaatccag
     1081 tgggagctta ttgccccgat tccccacgaa gccgcatctg agaagacagt aggcaaaaac
     1141 ggcaataccg aggcaagaaa ccagacagtc tgcaagcact gcggcgctgt gattgagtac
     1201 acaccccatc tgctttacga ctttgattta aactccaagg ttgacgcagc ggacgcgagg
     1261 attgtcctca gaatagccgc aaagcttgac aaggctacag aatcccacct tattgcatcc
     1321 ggcggcgaca agataaatcc gaatctttcg agaacaattc tgagaagagc cgcaaagctc
     1381 gactaaaggt aaaaatttaa gggcctctta aagaggccct gtttttttgt ttacagcgta
     1441 tccagttttg ccgcggctct taggattgcc ctcgcgtctg cggcgcttat gcccttgccg
     1501 tttacatcgg ctgagagctt ttgatattct gtgggactgt ccagttttgc cacaaaccgc
     1561 agcaccagtc ttgcgtcggc tgccgccgcc tgcccgtcgc cgtttacatc ccccaaaacg
     1621 gacagaagga gagtgtccga aactccgtca ttgccgatta gactgatttt cgaacccgtc
     1681 ggcacaattt cgctgcccga cagcagcttg cctttggaat ttttaacctc aattttgcct
     1741 gcttcaaagc tctttaataa atccgatact ttggtgtttg gcctaatatt gtaaacaatc
     1801 cctaatttgc tgtccagttt atagcccgaa tccgccttta gtttaagttt ggcattttcc
     1861 tcaacaacca cagtgcagga ggcggtgtag ccgccatcct ccgtaattgc ttttattgtt
     1921 gcggtgccgg actttaaagc ggtcacaacg ccgttttgac ttaccgtcgc aaccgaatta
     1981 ttgctcgatg tccagataat tttcttattg tcagcatttt ggggctttac ggtcgcaaac
     2041 aaagtttcgg ttttgcccgt tttgagtata acagcgtttt tgttaaggct gacacccgta
     2101 actttaatag gctttggctt tacggtaacc gtgcatgagg ctgtatagcc gccgtccatg
     2161 gtaacaaccg taatcaccgc cgtaccagca gtcttgcccg tcacaagccc gctttggctg
     2221 actgtggcaa ccagtgcgtt gctgctttca aaccgcacag acttattgtc ggcattgtgg
     2281 gggagaacct cataataaag ctgaaaattc tcgtctatca tcagctcaag gctgtttttg
     2341 ctcaaagtca cgccgctaac aggcacggga gcggctttta cggtaataac agcgctgccc
     2401 gttatcccgc cgtcctccgc aaccgctgtg acaacagcag ttccgattga gagcgcctta
     2461 atctcgcctt tttgattaac ggaaacaacg gcactgttgc cggaaatcca gtaaacattt
     2521 ttattccccg cttcttcggg aagtatcttt accgtcaaag tcctttcctc gcccacgctc
     2581 aaataaagct ccgagggctc gacaataaca tcggtaacat tctgaaaaaa ggact
//
LOCUS       DAONVQ010000173         2572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_27266_length_2572_cov_4.489074, whole genome shotgun sequence.
ACCESSION   DAONVQ010000173 DAONVQ010000000
VERSION     DAONVQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27266_length_2572_cov_4.489074"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /gene="nadD"
                     /locus_tag="PLD98_08670"
     CDS             <1..466
                     /gene="nadD"
                     /locus_tag="PLD98_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HQA06354.1"
                     /translation="PHKDNSELASAQDRLNMCRLAFRNELFEISDIELRREGKSYTVD
                     TLKEIKKERPGDKLFLIIGSDMLLSFHTWKDYREILNLATLAAAIREGESSRPLIDYK
                     NKFLQGHNVVILEFTPIPISSTEIREGISKAEIKEFLVPEVKEYIESRMLYR"
     gene            477..1037
                     /gene="yqeK"
                     /locus_tag="PLD98_08675"
     CDS             477..1037
                     /gene="yqeK"
                     /locus_tag="PLD98_08675"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HQA06355.1"
                     /translation="MEKLRELLSEKRFAHTMNVAKAAKELADIYGCDKEKAYLAALLH
                     DITKEFTKEQHFELIEKGNVKLTEIERTTQSLWHSITAPVYARLNLGIEDEDILNAIR
                     YHTTGRENMSTFEKIIFTADCISDDRSYPELDEVRSLAFTDLDAAVLAKLKYAIEFLV
                     KKDRPICGDTVSAYNELVMKKNERTE"
     gene            1038..1385
                     /gene="rsfS"
                     /locus_tag="PLD98_08680"
     CDS             1038..1385
                     /gene="rsfS"
                     /locus_tag="PLD98_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQA06356.1"
                     /translation="MSKELVEKIVNILDDKKAVDIEVIKIKELTIIADYFVIASGTSN
                     THLKALADELEFKLAEEDIRPRQIEGKATGWILLDYQNVVVHLFLPDARDYYNLSRLW
                     SDGSRIAVEELIH"
     gene            1418..>2572
                     /locus_tag="PLD98_08685"
     CDS             1418..>2572
                     /locus_tag="PLD98_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQA06357.1"
                     /translation="MEYNFKKVEKKWQDIWEESGVFHAKLDKSKEKFYCLVEFPYPSG
                     QGLHVGHPRSYTALDIIARKKRLQGYNVLYPMGWDAFGLPTENYAIKNKIHPEIVTRD
                     NIAKFKSQLKALGFSFDWSREINTTDPKYYKWTQWIFLKLFEKGLAYKKEMAVNWCTS
                     CKCVLANEEVVNGVCERCGNEVVRKVKKQWMLKITEYADRLIDDLDELDYIERVKLQQ
                     INWIGRSHGAQVDFTTTEGDTITVYTTRPDTLFGATYMVLSPEHPLIDKWADKLQNID
                     EINAYRDMAAHKSDFERTEINKEKTGVELKGVRAINPVNSKEIPIFISDYVLISYGTG
                     AIMAVPGHDTRDWEFAKKFGLPIIEVVSGGDVEKEAYTDIENGIMVNSGFL"
BASE COUNT          807 a          584 c          599 g          582 t
ORIGIN      
        1 ccctcataaa gataattctg agcttgcctc cgcacaggac agactgaata tgtgccgcct
       61 tgccttcagg aatgagcttt tcgagatttc cgacatcgaa cttcgccgcg agggcaaaag
      121 ctatacggtt gacaccctta aagaaattaa aaaggaaaga cccggagaca agctgttttt
      181 gattatcggc tctgatatgc ttctttcttt ccacacatgg aaggattaca gagagatact
      241 gaatctcgcc acccttgccg cggcaattag agaaggggag agcagccgcc ctcttatcga
      301 ttataaaaat aaattccttc aggggcataa tgttgttatt ctggagttta cccccattcc
      361 aataagctcc actgagataa gggaaggaat ctccaaggct gaaatcaagg aatttcttgt
      421 ccccgaagtg aaagaatata tagaaagcag gatgctttat agatagcaaa atttatatcg
      481 aaaagctaag agagctttta tccgaaaaac gctttgccca cacaatgaat gtggcgaaag
      541 cggcaaaaga gctggcagat atttatggct gcgacaagga aaaagcttat ctcgcggctc
      601 tgctgcacga cattaccaag gagttcacca aagagcagca ttttgagctt atcgaaaagg
      661 gtaatgtaaa gctcaccgag atagagagaa cgactcagag cctttggcat tcaataaccg
      721 ctcccgttta cgcaaggcta aatcttggaa ttgaagacga ggacatccta aacgcaatcc
      781 gctatcacac aaccggcaga gaaaacatga gcacctttga aaaaatcatc tttacggcgg
      841 actgcatttc cgatgacagg tcatatcccg aattggacga ggtaaggtct cttgccttta
      901 cggaccttga cgcggcggtt ctggcaaagc ttaaatacgc aatagaattc cttgtaaaaa
      961 aggacagacc catttgcggc gataccgtta gcgcatataa tgaactggta atgaagaaaa
     1021 acgaaaggac ggaataaatg agcaaagaac ttgtagaaaa aatcgttaat atactcgacg
     1081 acaaaaaagc tgttgatata gaggttatca aaatcaaaga gcttacaatc attgcggatt
     1141 atttcgtaat cgcctcgggc acatcaaaca cccacttaaa ggcgcttgcc gatgagctcg
     1201 agttcaaact cgctgaggag gatataagac cccgccagat tgaaggcaag gcaacgggct
     1261 ggattctcct cgactatcag aatgtggttg tccacctatt cctgcccgac gcaagagatt
     1321 actataatct cagccgacta tggtccgacg gttcaagaat cgcggttgaa gagctgattc
     1381 attaatatag attaattctg agacgggaga aatatagatg gaatataact ttaagaaagt
     1441 agagaaaaaa tggcaggaca tttgggaaga gtccggagta ttccatgcaa aactcgataa
     1501 aagcaaagaa aagttctatt gccttgttga gttcccttat ccttcgggtc agggtctgca
     1561 tgtaggtcac ccgaggtcct acaccgccct tgacatcata gcaagaaaga aaaggctcca
     1621 gggctacaat gttctttatc ccatgggctg ggacgccttc ggtctgccca cggaaaacta
     1681 cgccataaag aacaaaatcc atcccgaaat agtcacaagg gacaatatag caaagtttaa
     1741 aagccagctc aaagctctgg gcttctcctt tgactggagc cgcgagataa ataccaccga
     1801 ccccaagtat tacaagtgga ctcagtggat tttcttaaag ctcttcgaaa agggtctcgc
     1861 gtataaaaag gaaatggctg tcaactggtg tacctcctgc aaatgcgttc tcgctaacga
     1921 ggaggtcgta aacggcgttt gcgagcgctg cggcaacgag gttgtcagaa aggtcaaaaa
     1981 gcagtggatg ctcaaaatca ccgagtacgc cgacagactc attgacgacc ttgatgagct
     2041 tgactatatt gagagggtta agctccagca gataaactgg ataggcaggt cacacggcgc
     2101 tcaggtcgat ttcaccacaa ccgagggcga caccatcacc gtttacacca caagacccga
     2161 tacccttttc ggcgcaacat acatggtgct gtcacctgag catcccctaa tcgacaaatg
     2221 ggcggataag ctgcagaaca tagacgaaat taacgcttac agggatatgg cagcccacaa
     2281 gagcgacttt gagcgcaccg aaatcaataa ggaaaaaacg ggcgttgagc ttaaaggtgt
     2341 tagggcaatc aatcccgtaa actcaaagga aatcccgatt ttcatctccg actatgttct
     2401 catctcttac ggtaccggcg ctattatggc ggttcccgga cacgacacaa gagactggga
     2461 gttcgctaag aaattcggtc ttccgattat cgaggtcgtc agcggcggag atgttgaaaa
     2521 agaggcatat accgacatcg aaaacggcat tatggtaaac tccggcttcc tc
//
LOCUS       DAONVQ010000174         2509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_28147_length_2509_cov_3.835778, whole genome shotgun sequence.
ACCESSION   DAONVQ010000174 DAONVQ010000000
VERSION     DAONVQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28147_length_2509_cov_3.835778"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLD98_08690"
     CDS             <1..711
                     /locus_tag="PLD98_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQA06358.1"
                     /translation="PYLAAKSKKMMKRAKAIEERSFRAAEEKSKLLKNIETAENLKIS
                     QLNYHSSRLISLREISISYGENTVCEGVSFDIEKGDRIALQGKNGSGKSSILKMIMGE
                     DIPHTGEFQKGSGIIISYVPQDTSFLCGNLSDFALEHGIDESLFKAILRKLDFSRAQF
                     DKDMRDFSGGQKKKVLIAKSLCEKAHLYIWDEPLNFVDVISRIQIEELLLSFEPTILF
                     VEHDRVFCEIIANKTVNL"
     gene            complement(749..2005)
                     /locus_tag="PLD98_08695"
     CDS             complement(749..2005)
                     /locus_tag="PLD98_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HQA06359.1"
                     /translation="MSAVDSIRKTIGDTAWFTHDRFGMFIHWGLYALPARHEWIKNIE
                     RIKDEDYDKYFKYFNPDLFDASEWAKKAKAAGMKYAVLTSKHHEGFCLFDSKYTDYKS
                     TNTKFGRDIVKEFVEAFRAEGLKVGFYYSLIDWHHPDFTIDGKHPRRDDDNIEELNRG
                     RDMARYREYMKNQVTELLTNYGKIDILWFDFSYSAETYKGMKGKGKDDWDAEELIKTA
                     RSLQPDIIINNRTEIEQDIWTPEQYQPEEWIRDANGQRVVWEACQTFSGSWGYHRDEA
                     TWKSPEQLVRMLINTVSCGGNLLLNVGPTARGIFDERADFALEQIGKWMRLNSRSIYG
                     CTQSEFPASPDVRYTQNGKVLYAHIYAYPFVALRLKGLGGEVDYMQFLHDASEVLWDE
                     DGEDIIVKLPVVKPNQIVPVIEIFLK"
BASE COUNT          595 a          679 c          617 g          618 t
ORIGIN      
        1 ccctaccttg cggcgaaatc caagaaaatg atgaagcggg caaaggcgat agaggagcgt
       61 tcctttagag ccgctgaaga aaagtcaaag ctccttaaaa atattgaaac cgcggaaaac
      121 cttaaaatct ctcagctaaa ctaccactca agccgcctta taagccttag ggaaatttca
      181 atcagctacg gcgaaaacac cgtctgcgaa ggcgtatctt tcgacatcga aaagggcgac
      241 agaatcgccc ttcagggcaa aaacggctcg ggcaagtcca gcatattaaa aatgattatg
      301 ggcgaggata taccccatac gggcgaattc caaaagggca gcggcattat tatctcttat
      361 gtcccccagg atacctcctt cctttgcggc aatctttctg actttgcctt agagcacggc
      421 atagatgaga gcctgtttaa ggcaatcctt cgtaagctcg acttttcaag agcccagttt
      481 gacaaggaca tgagggattt cagcggcggg caaaagaaaa aggtgctgat cgctaaaagc
      541 ctttgcgaaa aagcccacct ttatatctgg gacgaaccct taaactttgt ggatgtcatt
      601 tcccgcattc agatagagga gctgcttctt tcctttgagc ctacaatcct ctttgtcgag
      661 cacgacaggg tattctgcga aataatagcg aacaaaacgg ttaatctata aaaatagggc
      721 ttaaagcgtt tgctttaagc ccttcttctt atttcaggaa tatctcgatt acgggaacta
      781 tctgattcgg ctttacaacg gggagcttta caatgatgtc ctctccgtcc tcatcccata
      841 aaacctcgga ggcgtcgtgc aggaactgca tgtagtcaac ctcgccgcca agacccttga
      901 gtctaagcgc cacaaaggga tatgcgtaaa tgtgagcgta gagcaccttg ccgttttgag
      961 tgtagcgcac atcgggcgac gccgggaact cgctttgagt gcagccgtaa atgcttctgg
     1021 aattaaggcg catccacttt cctatctgct caagagcaaa gtccgctctt tcgtcaaaga
     1081 tgcctctcgc cgtggggccg acatttaaaa ggagatttcc gccgcatgaa acggtgttta
     1141 taagcatcct gacaagctgt tcgggggatt tccaggttgc ctcatccctg tggtagcccc
     1201 atgagcctga gaaggtctgg caagcctccc aaacaacacg ctgaccgttt gcgtccctaa
     1261 tccattcctc gggctgatac tgttcgggag tccatatatc ctgctcaatc tcggttctgt
     1321 tgttgatgat tatgtcgggc tgaaggctcc tcgcggtctt tatcagctcc tcagcgtccc
     1381 agtcgtcctt gcccttgccc ttcatgcctt tgtaggtctc ggccgaatag gagaagtcaa
     1441 accacagaat atcaatcttg ccgtagttgg taagcagctc ggtgacctga ttcttcatgt
     1501 actccctgta gcgggccatg tccctgccgc ggttaagctc ctcgatattg tcgtcgtcgc
     1561 ggcgggggtg ctttccgtca attgtgaaat cgggatgatg ccagtcgatg agggaatagt
     1621 aaaagcccac tttaagacct tccgccctga acgcctcaac aaattccttg actatatccc
     1681 tgccgaactt tgtgttggtg gacttgtagt ctgtgtattt ggagtcaaag aggcagaagc
     1741 cctcgtggtg ctttgatgtc aaaaccgcgt acttcatgcc cgccgcttta gccttcttag
     1801 cccactctga ggcgtcaaaa aggtcggggt tgaagtattt aaagtatttg tcgtagtcct
     1861 cgtctttgat tctttcgatg ttcttaatcc attcatgcct tgcgggcagg gcgtaaagac
     1921 cccagtggat gaacatgccg aacctgtcgt gggtaaacca tgccgtatcg cctatggttt
     1981 tgcggatgga atcaactgct gacataaagt gcctccgttc aaaaattctt gtatagatag
     2041 attaaagaac attttcgaca atttatcaag cataatttgt aaacagtgcg ttgccgaaaa
     2101 cttaacttgt ccgctcgagc gcaagagctc cgctaagccc ctgcctgccg ccccaaccgc
     2161 agcgggggag gggctttttt cttccccgac gagggaaaac gcccactaag ggacacgccc
     2221 tgcgccatcc cccgagtaca gccatccgca aacaggggag cggggcagct tttccccacc
     2281 ccgccgccga cggcgacctt gccgcccgtt ccgcggctgc tgcccatctg tgacgggtcg
     2341 agcctcgcct gatcgttgta ctgcatcaga tcctcccttg gacggcgccg accacgcgag
     2401 catactgcct agccgaagcg cctgaacaag gcccttgtgg cgactcggtg tcgcgggatt
     2461 cctgggcgcc gatcatggcc gcaccgcatc tcgccgatcc gggcacccg
//
LOCUS       DAONVQ010000175         2503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_28226_length_2503_cov_4.669526, whole genome shotgun sequence.
ACCESSION   DAONVQ010000175 DAONVQ010000000
VERSION     DAONVQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28226_length_2503_cov_4.669526"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="PLD98_08700"
     CDS             <1..1133
                     /locus_tag="PLD98_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA06360.1"
                     /translation="FLRTQYKSVEYIMNSVGMISSFILVSFSLGYFNMELMTPALRNK
                     FMILGFILCLWFSVPLLFTFKYTKEPSSLHMPVPELDLRALAMEFVQVFKNKAFRQYF
                     LISVLYMMCRGFYGNSNVFFIRYIAKKPEKYNILTTIAGVAEASGFPLNYALTKKYGK
                     QKCGKLLTPIMMAGILLNLVINEALVGLTTDVFGLFKIDWVFVVVLCVAVLYNFGYSG
                     IGFVTTNIQPDVTDVDELITGRRREGVIATFSSFIKKTISGLMSGFTGLILKIFGFET
                     GKGTLYQTARAQRGLRITYILLPTIFLTLSYISLFRYKMTKSQHEMIKAAIAQKKKTG
                     KADLTEDEIRQIEEIAGQKFEDMWIGQEIDVPAIPDEAAELV"
     regulatory      1247..1427
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            1549..2403
                     /locus_tag="PLD98_08705"
     CDS             1549..2403
                     /locus_tag="PLD98_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQA06361.1"
                     /translation="MKKRFLMLIAATLAVILMLSGCAKTDKPDATDGASSPTASDNTF
                     KVGLEAGYAPFNWTQMDNSNGAVKIQNAAEYAGGYDVEIAKRIAEHLGKELVIVKTEW
                     NGLIPALTSGKIDAIIAGMSPTAERMESIDFSDVYYESDLVIVVKKGGKFENAKSLND
                     FAGAKITAQLNTFHYTVIDQIPGVVKREAMDDFPAMRVALESGIIDGYVTERPEAVSA
                     EAANKNFKMIELNPGFETNPEDTAIAVGIRKGSELKDQINEALAKISLDERKQLMDNA
                     IKNQPAAE"
BASE COUNT          688 a          585 c          592 g          638 t
ORIGIN      
        1 atttcctaag gactcagtac aaatcggttg aatacataat gaactccgtc ggaatgattt
       61 cctcatttat actggtttca ttctcactcg gttatttcaa catggagctt atgaccccgg
      121 cgctcagaaa caaattcatg atactgggct ttatactttg tctgtggttc tcggtgcctc
      181 ttttgtttac ctttaagtac acaaaggagc ccagctccct gcatatgccc gttccggagc
      241 tggatttaag agcacttgcc atggagttcg ttcaggtttt caaaaacaag gcattccgtc
      301 agtacttcct tatcagcgtg ctttacatga tgtgcagggg cttttacgga aactcaaatg
      361 tgttctttat tagatacatc gccaaaaagc ccgagaaata caatattctc accacaatcg
      421 ccggcgtcgc ggaggcttcc ggcttcccgc ttaactacgc cctgaccaaa aaatacggca
      481 agcagaaatg cggaaagctg ctgacgccca taatgatggc gggcatactc ctgaaccttg
      541 tcatcaacga ggcccttgtt ggtttaacca cagatgtgtt cgggcttttc aaaatcgact
      601 gggtgtttgt agtggtgctt tgcgtagctg tgctttacaa cttcggctat tcgggcatag
      661 gctttgttac cactaacatt cagcccgatg tcactgatgt tgacgagctg attacaggta
      721 gacgccgcga gggcgttatc gcaaccttca gctcatttat taagaagaca atcagcggtc
      781 ttatgtcagg ctttaccggt cttatactga aaatcttcgg atttgaaacg ggcaagggca
      841 ccctttacca gaccgcaaga gcccagaggg gccttagaat cacctacatt ctcctgccca
      901 ccattttcct taccctctcc tacatctctc ttttcagata caaaatgaca aagtctcagc
      961 atgagatgat aaaggctgcc atagctcaga agaaaaagac gggcaaggca gaccttaccg
     1021 aagacgagat aaggcagatt gaggaaatcg cgggtcaaaa gttcgaggat atgtggatag
     1081 gtcaggaaat agatgttccc gcaatccccg atgaggctgc cgagcttgta taaccgtatt
     1141 tatagtaaat ttttgcaatt atgcgatttt ctaaaaaatt tgttgacaca gagagtgtca
     1201 tattgtatac ttcttatacg aaaatttaat agaaacctca cttttaaagt gaggtagagg
     1261 cgcggtattt attagactcg gaggagcggg agcagcgttg atgccgaggt cgaggaaata
     1321 tcgccgaagt atataacatg ctcggtgtta tatgctgggt ctgcagtgaa taactgcagg
     1381 actgtctcaa taaatagccc tatttattga gttgcgctat ctcatttagg gaaagtaggg
     1441 ggtttaggca atattactgc atatttttgc agtagataca tagcatacaa gccacccgta
     1501 cagagggtgg cttttttagt aaaaatctaa tcagaaggac gaggaattat gaagaaaagg
     1561 tttttaatgt taatcgcggc aacactggca gtaattctga tgctttccgg ctgcgccaag
     1621 acggacaagc ccgacgccac agatggagca tcctccccca cagcatcgga caataccttt
     1681 aaggtaggtc ttgaggcagg ctacgcaccc ttcaactgga ctcagatgga caactccaac
     1741 ggcgccgtta agattcagaa cgctgcggaa tatgccggcg gctacgatgt tgaaatcgca
     1801 aagcgcatcg cagagcatct gggcaaagag cttgtaatcg tcaaaaccga gtggaacggt
     1861 cttatccccg cgctgacctc gggcaaaatc gacgccatca tcgcaggtat gtcgcccacc
     1921 gctgagcgta tggagagcat cgacttctcc gatgtttact atgagtcaga ccttgttata
     1981 gtcgttaaga agggcggcaa gtttgagaac gccaagtccc tcaacgactt tgcaggagca
     2041 aaaattaccg ctcagctcaa caccttccac tacacggtca ttgaccagat tcccggtgtt
     2101 gtcaagcgcg aggccatgga cgacttcccg gcaatgagag tcgcccttga atccggcata
     2161 atcgacggct atgtaactga gcgccccgag gctgtcagcg cagaggccgc caacaagaac
     2221 tttaagatga tagaacttaa cccgggcttt gagaccaacc ccgaggatac cgcaatcgca
     2281 gtcggcatca gaaagggcag cgagctcaag gaccagatta acgaagcttt ggcaaagatt
     2341 tctcttgatg agagaaagca gcttatggat aacgccataa agaatcagcc cgctgctgaa
     2401 taatagcgtt ttttcaaacc gggtgcattt tatgcgctcg gtttttgcca attcccggag
     2461 gtgtaatttt tggaatttca atttatccta aagaccatag caa
//
LOCUS       DAONVQ010000176         2500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_28264_length_2500_cov_3.964417, whole genome shotgun sequence.
ACCESSION   DAONVQ010000176 DAONVQ010000000
VERSION     DAONVQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28264_length_2500_cov_3.964417"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..>2500)
                     /locus_tag="PLD98_08710"
     CDS             complement(259..>2500)
                     /locus_tag="PLD98_08710"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQA06362.1"
                     /translation="EELVVGDVVLLEAGDAVPADGRILSCASMKVEEAALTGESVPVS
                     KTEETLSGGGSDDIPLGDRKNMVYMGSTVVYGRGTAVITATGMDTEMGKIADALATAE
                     EGQTPLQLKLAQLSKILSLLVLGICLFMFIFTIVIQRKTEFIDILNVFMLAVSLAVAA
                     IPEGLATVVTIVLSIGVTNMSKRNAIIRKLTAVETLGCAQIICSDKTGTLTQNKMTVV
                     EHFGDDVDLLATAMALCSDAELLEDGSIVGEPTEAALVSFAKEKGHPKPELKAKAPRI
                     GEAPFDSNRKMMSTVHNINGKIYQYTKGAPDEVLKRCTAYISGGEIKPMTDTMRAQIA
                     NANKNLASKALRVLCAAMRIHNEAPASFEPEALERDLIFIGLTGMIDPVRPEVIDAVK
                     ECKEAGIRPIMITGDHKDTAVAIAKQLGLLESEEAAITGTELNMLNDEEFENSIEKYS
                     VYARVQPEHKSRIVNAWRQKGYVTAMTGDGVNDAPSIKNADIGVGMGITGTDVTKNVA
                     DMVLADDNFATIVNAVEEGRRIYDNIRKAIQFLLASNLSEVLVIFAATLMGFTILYPV
                     HLLWINLITDAFPALSLGFEKAEKNIMKRKPRNPKDGVFAGGIGFDIAYQGIAVAILT
                     FAAYFIGHYIETKTWTATNSADGTTMAFLTLSMAEIFHSFNMRSQRGSIFSVKGQNKF
                     LWFAAISSFLMTCLVIYVPFLSNAFNFTHISLEEFAVAMVLAFAIVPIVEIVKLITRL
                     ATKKKS"
BASE COUNT          580 a          626 c          619 g          675 t
ORIGIN      
        1 cctaacataa gcataatgcc attatatcgc ccgattaata ccgcatttaa agcatataaa
       61 agcattttcg ctaattttca tccctattgc taatatctaa tccggcgaaa gcgatttcac
      121 cgcagcttgc ctgcgaatat caaaagggtc tctcttgtta atagagagcc aaaaagagca
      181 ggggagctgt aatacccctc taaagcctta aaaaagcgca aaaaaacagc cgctgcgttt
      241 tgcagcggct aatttaatct atgatttttt ctttgtcgca agccttgtta taagctttac
      301 gatttctaca atcgggacaa ttgcgaatgc cagaaccatc gctaccgcga actcctcaag
      361 gctgatgtgg gtaaagttga acgcgttgct gaggaaggga acatagatta caaggcaggt
      421 cattaagaag ctggagatgg ctgcaaacca caggaacttg ttctgaccct ttaccgagaa
      481 aatgctgcct ctctggctgc gcatattgaa gctgtggaaa atctccgcca tgctcaaagt
      541 cagaaacgcc atcgtggtgc cgtctgcgct gtttgtggcg gtccatgtct ttgtttcgat
      601 gtagtgacct ataaagtaag cggcaaatgt caatatggca accgcaatac cctgatatgc
      661 gatgtcaaaa ccgataccgc cggcaaatac accgtctttg ggatttctgg gcttgcgctt
      721 cattatattc ttctcagcct tttcaaagcc gagggagagt gcggggaatg cgtctgtaat
      781 aaggttaatc cagagaaggt gaacgggata gagaattgta aagcccatga gcgttgcagc
      841 aaagataacc agaacttcac tgaggttgga ggcaagcagg aactgaattg cctttctgat
      901 attgtcgtag attcttctgc cttcttcaac cgcgttgact attgttgcaa agttgtcgtc
      961 ggcaagaacc atatcagcaa cattcttggt aacatccgta ccggtaatgc ccatgcccac
     1021 gccgatgtcg gcgttcttaa tgctgggcgc gtcgtttacg ccgtcgccag tcatggcggt
     1081 tacatagccc ttttggcgcc atgcgttgac tatgcggctc ttgtgctcgg gttgaacgcg
     1141 ggcataaacg ctgtatttct caatgctgtt ttcaaattcc tcgtcgttga gcatattaag
     1201 ctctgtgcct gtaattgcgg cctcttcgct ttcaagaagt cccagctgtt ttgcgattgc
     1261 aacggcggta tctttgtggt cgcctgtaat cattatcggg cggatacctg cctccttgca
     1321 ctccttaact gcgtcgataa cctcggggcg tactgggtca atcatgcccg taagacctat
     1381 aaagataagg tcgcgttcaa gagcctcggg ctcaaatgag gcgggtgcct cgttatgaat
     1441 tctcatagca gcgcagagaa ctctcaaagc cttgcttgca aggttcttgt tggcgtttgc
     1501 aatctgagcc ctcattgtgt cggtcatggg cttgatttcg ccgccgctga tatatgctgt
     1561 acaccttttg aggacctcat cgggagcgcc cttggtatac tgatatatct tgccgtttat
     1621 gttgtgaacg gtgctcatca tctttctgtt gctgtcaaag ggagcctcgc cgattctggg
     1681 agcctttgcc ttaagctcgg gcttgggatg acccttttcc tttgcgaagc tgactaaagc
     1741 cgcctctgtg ggctcgccga caatgctgcc gtcctcaagg agctctgcgt cgctgcacaa
     1801 agccatggca gtcgcaagca ggtccacatc atcgccgaaa tgctcaacaa cggtcatctt
     1861 gttttgtgta agtgttcccg tcttgtcgct gcatattatc tgagcgcagc ccagggtttc
     1921 aactgcggtc agctttctta taatggcgtt tctctttgac atgttggtaa cgccgatcga
     1981 aagaactatt gtaacaaccg tcgcgagacc ttcggggatg gcggcaaccg caaggcttac
     2041 cgcaagcata aagacattaa gaatatctat aaactcggtc tttctctgaa ttacgattgt
     2101 aaagatgaac ataaagaggc agatgccgag tactagaagg ctgagtatct tgctgagctg
     2161 agcgagcttg agctgcaggg gagtctggcc ctcctcagcg gtggcaaggg cgtcggctat
     2221 cttgcccatt tcggtatcca tgcccgttgc ggtgataact gctgtacctc tgccgtagac
     2281 aacggtgctg cccatgtaaa ccatgttctt gcggtcaccg agcggaatat cgtcgctgcc
     2341 gccgccgctc aatgtctctt ccgttttgct tacgggaacg ctctcaccgg taagcgcggc
     2401 ttcttccact ttcatgctgg cgcagctaag tattctgccg tcggcgggta cagcgtcgcc
     2461 tgcttcaagc agaacaacat cgccgacaac aagctcctcg
//
LOCUS       DAONVQ010000177         2437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_29231_length_2437_cov_4.133921, whole genome shotgun sequence.
ACCESSION   DAONVQ010000177 DAONVQ010000000
VERSION     DAONVQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29231_length_2437_cov_4.133921"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLD98_08715"
     CDS             complement(<1..258)
                     /locus_tag="PLD98_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06363.1"
                     /translation="MGKEGVNSIAKIDNLEKKDIKSYDLVELKALMGEIGQPAFRAGQ
                     VFRWLHKECVQSFEEMTNLPLNLRQQLNDNFVIIGAAIEKKL"
     gene            complement(357..1700)
                     /gene="rsmB"
                     /locus_tag="PLD98_08720"
     CDS             complement(357..1700)
                     /gene="rsmB"
                     /locus_tag="PLD98_08720"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HQA06364.1"
                     /translation="MSTGREAAYKILLKIEKDSAYSNLALKDFFKDSTLPPNEKALSY
                     NIVLGVLDRRLTLDFILSRYLAKPINKLDPKVLTLLRMGSYQILYMDKIPDFAAINET
                     VKQAAKIAAYSKGLVNAVLRKVAKKEPLNINKEENPVLYYSIRYSFPEWLVKLFIESY
                     GEENAEGIMAASLQAGETVCCANPLKTSRDELIKNLAAKGIEAAEDNRLKDAFYIKGS
                     VNLSELREFKDGLFAVQDISSQLCAEAVGAKEGDTVIDVCAAPGGKSIRLSQLMNNKG
                     RIISCDIHEHKTKIIESYAKRMGADIISAHARDAASPDTCIDGEDFRGKADRVLCDAP
                     CSGFGVIRGKPEIKYKPKEEIEKLPELQFKILHSSAQLVKKGGTLVYSTCTLNPAENE
                     RVCDRFLSENGEFEAIKPLEGALGGEIKYGESNYLTLLPHIYNTDGFFIAAFRRK"
     gene            complement(1857..>2437)
                     /locus_tag="PLD98_08725"
     CDS             complement(1857..>2437)
                     /locus_tag="PLD98_08725"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HQA06365.1"
                     /translation="ATNGLTGAQAAAEVLRYYGITNVQITSIPGKLTDHFNPKTNTIG
                     LSESVYRGTSVADIGIACHEAGHAAQYAHSYLPIKLRNMILPIANIGSFAGIPLALIG
                     FVLQWQGLVTFGLMLYAAIAVFQFITLPVEINASRRALKVINETGILVTPEERAGARS
                     VLFAAAMTYIAALAVSLANLVRFIALFGRRNR"
BASE COUNT          558 a          564 c          572 g          743 t
ORIGIN      
        1 gagttttttt tcaatagctg cgccaattat gacaaaattg tcattaagct gctgccttaa
       61 attcaggggg agattggtca tttcctcgaa agactgaacg cactctttgt ggagccagcg
      121 gaaaacctga cccgcgcgaa aggcgggctg acctatttcc cccattaggg ctttaagctc
      181 caccaaatcg tatgatttta tgtccttttt ctctaaatta tctatcttag ctatggaatt
      241 tacgccttcc ttccccacct gtggactttg ctttattttt attattgaga aatttgcttg
      301 attttatatc atttataact ctttttgcgc acttgtccgc tttcaaagcc gaaaaactat
      361 tttctcctga aagcggcaat gaaaaatccg tcggtgttat aaatatgcgg cagaagtgta
      421 agataattcg attcaccgta tttgatttcg ccgcccaaag ctccttcaag aggttttatt
      481 gcctcaaact cgccattttc cgaaaggaat ctgtcgcaga ctctttcgtt ctcggcggga
      541 ttcagggtgc aggttgagta taccagagtg ccgccctttt ttacaagctg agcgcttgag
      601 tggaggattt taaattgcag ctcggggagc ttttcgattt cttccttggg tttatactta
      661 atttcgggct ttcctctgat aacgccaaag cctgagcagg gcgcgtcgca gagaaccctg
      721 tcagcttttc ctctaaaatc ctcaccgtct atacaagtgt ctggggatgc agcgtctctt
      781 gcatgagcgg aaattatatc ggctcccatt cttttagcgt agctttctat gattttagtt
      841 ttatgctcgt gaatgtcgca ggagattatc ctgcccttgt tgttcataag ctgagagagc
      901 cttatgcttt ttcctcccgg ggcggcgcag acatcaatga ccgtatcgcc ctcttttgcc
      961 ccgacagcct cagcgcaaag ctgtgaggaa atatcctgaa cggcaaagag tccatcctta
     1021 aactccctca gctctgagag attgaccgat ccctttatat aaaaggcgtc tttaagtctg
     1081 ttatcttcgg cagcctcaat gccctttgcc gcaaggtttt ttataagctc atccctgctt
     1141 gtttttaagg gattcgcgca gcagacggtt tcgcccgcct gcaaagaggc ggccataatg
     1201 ccctcggcgt tttcctcccc gtatgattca ataaagagct ttacaagcca ttcgggaaag
     1261 gagtatctga tgctgtaata aagaacgggg ttttcctctt tgtttatgtt taagggttct
     1321 tttttcgcga cctttctcaa aaccgcgttt acaagaccct ttgaataggc ggctatcttt
     1381 gcggcttgct ttaccgtttc gttaattgcg gcaaagtcgg ggattttgtc catgtatagg
     1441 atttgatacg agcccattct gagaagggtc aaaaccttgg ggtcaagttt gtttatgggc
     1501 tttgcaaggt atcgggaaag tatgaagtca agggtcagac gcctgtcaag cactccgaga
     1561 acaatattgt atgagagggc tttttcgttt gggggaagcg tggaatcctt gaaaaaatct
     1621 ttaagggcga gatttgaata cgccgagtct ttctctatct ttaaaagtat tttgtatgcc
     1681 gcttccctgc ctgtgctcac aaatccgccc cctatagctt tctatcttta aaatttaggc
     1741 gctttttgcc gtcttataca gttttccggt tttatacagc atagagccat tttactttat
     1801 accacatata aagcggcatg gcaagtgcca tgccgctgag gtggtccgta ggctttttat
     1861 ctgtttcttc tgccgaacaa tgctataaat cttacaaggt ttgcgaggga taccgcaagg
     1921 gcagcgatgt atgtcattgc cgcggcgaaa agcacactcc ttgcccctgc tctttcctcg
     1981 ggtgtaacga gaattccagt ttcgtttatg accttcaatg cccttctgct ggcgttgatt
     2041 tcaacgggaa gggtgatgaa ctggaatacg gcgattgcag cgtaaagcat aagtccgaaa
     2101 gttacaaggc cctgccactg aagcacaaag ccgattaaag caaggggtat gccggcgaat
     2161 gagccgatat tggcgatggg aagtatcatg ttcctgagct ttatcggcag gtagctgtga
     2221 gcatactgcg cggcgtgacc cgcctcatgg caggcgattc ctatatccgc aacgcttgtg
     2281 cccctgtaaa ccgattcgga aagacctatg gtgttggtct tggggttaaa gtggtcggtg
     2341 agctttccgg gtatgctggt gatctgtaca tttgttattc cgtaatatct caaaacctcc
     2401 gcagccgcct gggcgcccgt aaggccgttt gtggcaa
//
LOCUS       DAONVQ010000178         2416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_29567_length_2416_cov_3.688268, whole genome shotgun sequence.
ACCESSION   DAONVQ010000178 DAONVQ010000000
VERSION     DAONVQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29567_length_2416_cov_3.688268"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PLD98_08730"
     CDS             <1..643
                     /locus_tag="PLD98_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQA06366.1"
                     /translation="KGMVVHPAAGNPDGTLVNALLHHCKGRLSSINGVIRPGIVHRID
                     KDTSGLLIVAKTDSAHNGLAKQIKEHSFKRIYDAVVIGRLKNTSGTINVPIGRSPKDR
                     KKRAVNGLNPKPAITLYNVVNYYEGYTHVRFELKTGRTHQIRVHAAYIGHPVAGDTVY
                     GPSKSKLPFLNGQCLNATVIGFVHPITGEYLEFDSGLPDYFTGFLNRLTPSEI"
     gene            662..1516
                     /locus_tag="PLD98_08735"
     CDS             662..1516
                     /locus_tag="PLD98_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQA06367.1"
                     /translation="MDIKEKRQLEITALKIRMGVIEGTFNAKSGHPGGSLSLTDILTY
                     LYFKEMNIDPKNPKWEDRDRFVLSKGHAAPGLYSALAHRGFFPVEHLKTLRKADSDLQ
                     GHPSMKSTPGVDMSTGSLGQGISAAVGMALGGKHAGKNFRVYAALGDGELGEGQVWEA
                     AMFAAAKGLENLTIFVDNNNLQIDGTVDEVNSPYPIPEKFVSFGFNVIEIDGHNFDEI
                     ESAINIAKATKGRPTAIIAKTVKGKGVSFMENSCDWHGKAPNAEEYELAMNELKTKLQ
                     KLEESANV"
     gene            1509..>2416
                     /locus_tag="PLD98_08740"
     CDS             1509..>2416
                     /locus_tag="PLD98_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HQA06368.1"
                     /translation="MSDVIKVATREAYGAALVELKKVNDSVLVLDADLSAATKTGTFK
                     KAYSESFYNCGISECNMMGIAAGLSTIGYTVFASTFAMFAAGRAFEQIRNTIGYPHLN
                     VKIGATHAGISVGEDGASHQCCEDIALMRTIPGMTIINPADDVEAKAAVFAAAEIEGP
                     VYLRFGRLPVPRIFDENYKFEIGKGVLLREGTDVTIIATGLMVERALKAAELLSASGI
                     SAAVINMPTIKPLDKDIVIEAAKKTGAIVTAEEHSIIGGLGGAVAEAVSETVPVPVLR
                     VGVEDVYGRSGPALELLEIYGLNAEN"
BASE COUNT          668 a          561 c          601 g          586 t
ORIGIN      
        1 aaagggcatg gttgttcacc ccgccgccgg caatcccgac ggcacccttg taaacgctct
       61 tttgcaccac tgcaagggca ggctttcaag cattaacggt gttattcgcc cgggtatagt
      121 gcatagaatt gacaaggaca caagcgggct tttaattgta gccaagaccg acagtgctca
      181 taacggactt gcaaagcaga ttaaagagca cagctttaaa aggatttatg acgctgttgt
      241 tatcggaagg ctaaaaaaca caagcggcac tataaatgtc cccattggca gaagccccaa
      301 ggacagaaaa aagcgcgcgg taaacgggct gaatccaaag cctgcgataa ctctttataa
      361 tgtagttaat tattatgaag gttataccca tgttaggttt gaattgaaaa cgggcagaac
      421 tcatcagatt agggttcacg cggcatatat cggtcacccc gtagcgggcg acacggttta
      481 cggaccctct aagtcaaagc tgcctttctt aaacgggcag tgccttaatg ctacggttat
      541 tggttttgtt catccgataa cgggtgagta ccttgaattt gattcggggc ttcccgatta
      601 tttcacaggc tttttaaaca ggctaacccc atccgaaatc taattaagga gtgcatttaa
      661 agtggatatc aaagaaaaaa gacagctgga aatcaccgct ttaaaaataa gaatgggcgt
      721 aattgaaggc acttttaacg ccaaatcggg tcaccccggc ggttctcttt cgctcaccga
      781 cattttaacc tacctttatt ttaaggaaat gaatatcgac cccaagaatc ccaagtggga
      841 ggacagggac agatttgttc tctcaaaagg acatgccgca cccggtcttt actcagccct
      901 tgcccacagg ggatttttcc ctgttgagca cttaaagact ttaagaaagg ctgactccga
      961 ccttcagggt catcccagca tgaagtccac tcccggtgtt gatatgagca caggctctct
     1021 cggtcagggc atttccgccg cagtaggaat ggctcttggc ggaaagcatg cgggcaagaa
     1081 tttcagagtt tacgcagcgc ttggtgacgg cgagcttggt gagggtcagg tctgggaggc
     1141 agctatgttt gccgccgcaa aggggcttga gaatctcact atctttgtag acaacaacaa
     1201 cctgcagatt gacggaaccg ttgatgaggt taactcaccc tatccgattc ctgaaaaatt
     1261 cgtatcattc ggattcaatg tcattgaaat agacggtcac aatttcgacg aaattgagtc
     1321 tgcgataaat attgcaaagg caacaaaggg ccgtcctaca gcaataattg caaagacagt
     1381 taaaggcaag ggcgtttcct tcatggagaa cagctgcgac tggcacggca aggcgcccaa
     1441 tgctgaggaa tatgagcttg cgatgaatga actcaaaaca aaacttcaaa agctggagga
     1501 aagtgctaat gtctgatgta attaaagttg caacaagaga agcctacggc gccgcgcttg
     1561 ttgaattaaa aaaggtaaac gattcggtat tggttcttga cgctgacctt tctgccgcaa
     1621 caaagacagg tacttttaaa aaggcatatt ctgagagctt ttataactgc ggcatttccg
     1681 aatgcaatat gatgggcatt gccgccggtc tttccaccat cggatatacg gttttcgcga
     1741 gcacatttgc gatgttcgcg gcaggcagag cctttgagca aatcagaaat acaataggtt
     1801 atccgcatct taatgttaaa atcggcgcaa ctcacgctgg catttcagtc ggcgaggacg
     1861 gcgcaagcca tcagtgctgc gaggatattg cccttatgcg caccatcccc ggtatgacaa
     1921 tcattaatcc cgcagacgat gttgaggcaa aggccgccgt atttgccgcc gctgaaattg
     1981 aaggccccgt atatcttaga ttcggtagac ttcccgttcc gaggattttt gacgagaatt
     2041 ataaattcga aatcggcaag ggcgttctgc taagagaagg taccgatgtt acaatcatcg
     2101 cgacaggtct tatggtagag cgcgcgttaa aggccgcgga gctcctttcc gcttcgggca
     2161 tttccgccgc tgttatcaat atgcccacaa tcaagccttt ggataaagac attgttattg
     2221 aggcagcaaa gaagactgga gcaatcgtca ccgctgagga gcacagcata atcggcggac
     2281 tcggcggcgc tgttgctgag gctgtcagcg agacagttcc cgtccccgtt ctcagggtcg
     2341 gcgtagaaga tgtttacgga cgctcaggtc ccgctcttga gctgcttgaa atctacggac
     2401 ttaacgcaga aaacat
//
LOCUS       DAONVQ010000179         2352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_30619_length_2352_cov_3.141924, whole genome shotgun sequence.
ACCESSION   DAONVQ010000179 DAONVQ010000000
VERSION     DAONVQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30619_length_2352_cov_3.141924"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLD98_08745"
     CDS             <1..305
                     /locus_tag="PLD98_08745"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQA06369.1"
                     /translation="LRQLSGARHEVFTGVTIASPGKRISFFEQSYVYFNELSDEEIEA
                     YISSGEPMDKAGAYGIQGRGCVFVKRIEGDFFNIMGLPVAKVYDVLKSEFSYKPAL"
     gene            316..936
                     /locus_tag="PLD98_08750"
     CDS             316..936
                     /locus_tag="PLD98_08750"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQA06370.1"
                     /translation="MEQTNRTEEKEQLKMLKFFKENEESRLYQIPEGYYIRTAKLEDG
                     PAWCKCCLNGCLGVEECSVDLFKERMLDDPSVSLDNIFLICDSEGKIAGTATARLPIA
                     KHYPDAGILHMVAVADEYKGKGLAGPVCEAAVNRVKSMGYDKCFLTTDDHRIPAIKIY
                     IKLGFLPIINSDEMRARWENILKQININSIDALDENLSPIAPVETK"
     gene            955..1680
                     /locus_tag="PLD98_08755"
     CDS             955..1680
                     /locus_tag="PLD98_08755"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HQA06371.1"
                     /translation="MLNFVRLPLEGAYNVRDLGGYPTKCGRMTKWHAFLRSDDPYDLT
                     DSDIEFLKEYGLKTVIDLRAEDEMEAFPNPFERVEGVDYIKAPIFLDKIDYITSAASH
                     YKPQDMITAFYLHLLTEARENIRKVFSSIAEAKEGAVMFHCKVGKDRTGIVAALLLML
                     CGVSRADIITNYSSSFHYIIQNPQIVKMIQNSIPDLFYSKPEYIEPVLDYIEKEHGDA
                     KGYLMWIGLKEDEIKAIEKRLCE"
     gene            complement(1749..>2352)
                     /locus_tag="PLD98_08760"
     CDS             complement(1749..>2352)
                     /locus_tag="PLD98_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HQA06372.1"
                     /translation="RPGPAGPPGPTGPTGPEGPIGPTGPTGPEGPIGPTGPTGPEGPI
                     GPTGPTGPEGPTGPTGPTPELQGLQVQATGLGAATVDDGDNLIFDTVVNDAAADIDYD
                     DATGVFTIQADGLYYISWFVNTDGAGAATSVAFAIDVVGGPTIEASSPPPNVTTQLNG
                     TALITVAGAPVEFSLVNTTGDTVFIASTAVQAGLSIIKLS"
BASE COUNT          647 a          517 c          620 g          568 t
ORIGIN      
        1 tattaaggca gctctcggga gcgagacatg aggtttttac cggcgttaca attgcctcgc
       61 caggcaaaag aatcagcttc tttgagcagt cctatgttta ctttaatgag ctaagcgatg
      121 aggagataga ggcttatatc agttccggag agcctatgga caaggcaggg gcatacggaa
      181 ttcagggcag aggctgcgtt tttgttaaaa gaatcgaggg cgactttttc aacattatgg
      241 gtctgcccgt cgctaaggtt tacgatgtac taaagagcga attttcctac aaaccagctt
      301 tataggagga ataaaatgga gcaaacaaac agaacggaag aaaaagaaca gcttaaaatg
      361 ctgaagtttt ttaaggagaa cgaagaaagc cgcttatacc aaatacccga aggctattat
      421 ataagaacgg caaaacttga ggacggtccc gcatggtgta aatgctgcct taacggctgc
      481 ttgggcgttg aggagtgcag cgtggatttg tttaaggaaa gaatgcttga cgacccgtcg
      541 gtcagccttg ataatatttt ccttatctgc gacagcgaag gaaaaattgc gggcacagct
      601 acggcaaggc ttcccatagc caagcattat cccgacgcgg gcatccttca catggttgcg
      661 gtagccgacg aatataaggg caaaggtctt gcgggtcctg tctgcgaggc ggctgtgaac
      721 agagtaaaaa gcatgggcta tgacaaatgc ttcctcacaa ccgacgacca cagaatcccc
      781 gcgattaaga tttatattaa gctcggtttt ctccccatca taaacagtga cgaaatgaga
      841 gcaagatggg aaaacatatt aaaacagata aacattaatt caatcgatgc gcttgacgag
      901 aatctctcgc ccatcgctcc ggttgagacg aaatagcatc ggaaagaggt aaaaatgctg
      961 aacttcgtaa gactccccct tgagggagcc tataatgtaa gggaccttgg cggctacccc
     1021 acaaagtgcg gccgcatgac aaaatggcat gcctttttaa ggtcagacga cccgtacgac
     1081 cttacagaca gcgatattga atttttgaag gaatacggac ttaaaacggt aatagacctg
     1141 agggctgaag atgaaatgga ggctttcccc aacccctttg agagggttga gggtgtggat
     1201 tatattaaag ccccaatttt ccttgacaaa atcgactaca ttacaagtgc agcttctcac
     1261 tataaacccc aggatatgat tacagccttc tatcttcatc ttctgaccga ggcaagggag
     1321 aacataagaa aggtcttttc ttctatcgct gaggcaaaag agggagctgt aatgttccac
     1381 tgcaaggtgg gcaaggacag aacgggaatt gtggccgctc tcctgcttat gctgtgcggt
     1441 gtttcaagag cggacataat cacaaattat tcttcttcct tccactatat aattcagaat
     1501 ccccagatag ttaagatgat tcaaaactcc atacccgatt tgttttactc aaagcctgag
     1561 tacattgagc cggttttgga ttatatcgaa aaagagcatg gggacgcaaa gggctattta
     1621 atgtggatag ggttaaaaga ggacgaaatt aaggcgattg aaaaaagact atgcgaataa
     1681 agcaaaaggc gcagccattt ggccgcgcct tctattttta tatctaaacc ttagattatt
     1741 cctttaattt agctgagctt gattattgac agtccggcct gaacggcagt ggaggcgatg
     1801 aatacggtgt cgcctgttgt gtttacaagc gagaactcta caggagcgcc tgcaaccgta
     1861 atcagagcgg tgccgttaag ctgggttgtt acattcggcg gcggtgaaga ggcttctatt
     1921 gtaggacctc cgaccacatc gattgcaaat gctaccgatg ttgcggcgcc tgcgccgtcg
     1981 gtgttgacaa accagctgat ataatacagg ccgtctgctt ggatggtgaa tacgcctgtt
     2041 gcatcatcgt agtcgatgtc tgcggcggcg tcgttgacta cagtgtcaaa gattaagttg
     2101 tcaccatcgt caacagttgc tgcgccaagg cctgttgcct gaacctggag accctgaagc
     2161 tcaggtgtcg gaccggtagg acctgtggga ccttcgggac ctgtcggacc tgtcggacca
     2221 atcgggcctt cgggacctgt cgggcctgtc gggcctatcg gaccttcggg acctgtcgga
     2281 cctgtcggac caatggggcc ttcggggccg gtaggacctg tgggaccggg gggacctgca
     2341 ggaccgggtc tg
//
LOCUS       DAONVQ010000180         2318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_31194_length_2318_cov_4.342466, whole genome shotgun sequence.
ACCESSION   DAONVQ010000180 DAONVQ010000000
VERSION     DAONVQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31194_length_2318_cov_4.342466"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLD98_08765"
     CDS             <1..274
                     /locus_tag="PLD98_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06373.1"
                     /translation="SKGIYSVCGCFIKKMQGKIQIHFLSSIFILALLYSFLNTNARLL
                     FSKAVIFCTKNNNYKREKAQQELRFFHAVFCIGRGDCHFISMKAGT"
     gene            complement(252..932)
                     /locus_tag="PLD98_08770"
     CDS             complement(252..932)
                     /locus_tag="PLD98_08770"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HQA06374.1"
                     /translation="MYSTFQSAQVTLRSVERSFYYRGTEVLNVEMEYPVVSGIGNRAA
                     AIINGRIRSQVNSFWNYVRNQLYREAVSEYRNAMQNGYPFREFSAVLSYNVEYNRNCH
                     LSIFREMFQYTGGAHGTTEKRSDTWNIRTGRLIPISDYFERRADYKNIILTEAIRQAD
                     ADPSIYFENYAELMEQNFDPESYYLTDAGPTPGLVIYYQEYEIAPYASGTPIFTIPYS
                     MLKYPPSC"
     gene            complement(1053..2105)
                     /gene="leuB"
                     /locus_tag="PLD98_08775"
     CDS             complement(1053..2105)
                     /gene="leuB"
                     /locus_tag="PLD98_08775"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HQA06375.1"
                     /translation="MNFNLALLKGDGIGPEIVDSAIAVLNRICSKYNHTVKYNEYLAG
                     GCAIDAVGVPLPEETLKGCLESDSVILGAVGGPKWDTLPGHLRPEKALLGVRKALGLY
                     ANIRPATLYSALTERCPLRSAASFDIVIIRELTGGIYFGKSGREDAGQMGEAAFDTEY
                     YTVKEIERIGRVGFETALKRNKKLTSIDKANVLESSRLWRETMHRLAEEYPDVEYSDM
                     LVDNAAMQLIRDPAQFDVIVTSNMFGDILSDEASQLTGSIGMLPSASLGEGTRGMYEP
                     IHGSAPDIAGKGIANPLATILSVAMMLRYSFNLEAEAKEIENAVEEVLNKGFLTRDLG
                     GDKNTAEITEELLKLI"
     gene            complement(2105..>2318)
                     /gene="leuD"
                     /locus_tag="PLD98_08780"
     CDS             complement(2105..>2318)
                     /gene="leuD"
                     /locus_tag="PLD98_08780"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355784.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQA06376.1"
                     /translation="IGLPIIECPEAVADAENGDEIGMDFDSGVIVNYTKNKQYKAAAF
                     PPFIQAIIEKGGLVEAVNANIFGGKE"
BASE COUNT          540 a          531 c          570 g          677 t
ORIGIN      
        1 aagcaaaggg atttactctg tttgtggttg cttcatcaag aagatgcaag gtaaaatcca
       61 aatacatttt ttgtcctcca tttttattct cgctttatta tatagtttcc tcaatactaa
      121 tgcaagacta ttatttagca aagcggttat tttttgcaca aaaaataata attataaaag
      181 agaaaaagcg cagcaagaac tgcgcttttt ccatgctgtg ttctgtattg gtagggggga
      241 ttgtcacttt atcagcatga aggcgggtac ttaagcatac tgtaaggaat tgtaaagatg
      301 ggagtgcctg aagcataggg ggcaatctcg tattcctgat agtagattac aagtcccggg
      361 gtcggacccg cgtcagtcag atagtagctt tcggggtcga aattctgctc cataagctcg
      421 gcataatttt caaagtatat tgacgggtcg gcgtctgcct gacggattgc ctcggtcagg
      481 attatgttct tgtaatcagc tctcctttca aagtagtcgg aaatgggaat cagccttccc
      541 gttcttatgt tccatgtatc ggagcgcttt tcggtggtgc cgtgggctcc tcccgtgtat
      601 tggaacatct ctctgaaaat gctcaggtgg cagtttctgt tgtactccac attataggac
      661 agtacagcgg aaaactctct gaaggggtag ccgttctgca ttgcgtttct gtattcgctt
      721 acggcctccc tgtaaagctg gtttcttaca taattccaaa aggaatttac ctgacttctt
      781 attctgccgt taataatcgc ggcggctctg tttcctattc ccgatactac ggggtattcc
      841 atttcaacat taagcacttc ggtgcctctg taataaaagc tgcgttctac gctgcgcaat
      901 gtcacctgcg cgctctggaa tgtactatac attgtgatac ctccgactct ttattaccct
      961 gttacattct atgcaaaggg caaatttagg acacagaaag tcggagacaa aaaaataccc
     1021 ttagaaatct aagggcattt tggtttgtgc aattatataa gtttaagcag ttcttcggta
     1081 atttctgcgg tattcttgtc gccgccaagg tctcttgtaa ggaatccttt gttaagaact
     1141 tcctcaactg cgttttcaat ctctttagcc tctgcttcga gattaaagga gtatctgagc
     1201 atcatggcga ctgagagaat tgtggccaag gggtttgcta tgcccttgcc ggcaatgtcg
     1261 ggggcggagc cgtgaatggg ctcgtacatt ccccttgtgc cctcgccgag agaggcggag
     1321 gggagcatac cgattgagcc tgtgagctgg gaggcttcgt ctgagagaat gtcgccgaac
     1381 atatttgatg tgactataac atcaaactgc gccgggtctc ttataagctg cattgctgcg
     1441 ttgtcaacaa gcatgtccga atactcaaca tcggggtatt cctcggcaag acgatgcata
     1501 gtctctctcc aaagccttga gctttcaagg acattcgcct tgtcgatgct tgtgagcttc
     1561 ttgtttctct ttaaggcggt ttcaaagcct actctgccga tgcgctcgat ttcttttacg
     1621 gtgtagtact cggtatcgaa agcggcctcg cccatttggc cggcgtcttc tctgccgctt
     1681 ttgccgaagt aaatgccgcc tgtaagctct cttataatga ctatgtcaaa ggaggctgcg
     1741 ctccttaaag gacatctttc ggtaagggcg gaataaaggg ttgcgggacg gatgttggca
     1801 taaagtccaa gggcttttct gacacccaga agcgcctttt cggggcggag atgtccgggc
     1861 agtgtgtccc acttgggacc gccgacagcg cccagaatta cgctgtcgct ttcaaggcag
     1921 ccctttaagg tttcttcggg cagcggaacg cccactgcgt caatggcgca gccgcccgca
     1981 aggtactcgt tgtatttaac ggtatgattg tatttggagc agattctgtt aagaaccgca
     2041 atcgcgctgt caacgatttc ggggccgatt ccgtcgcctt taagcaaagc taagttgaaa
     2101 ttcattattc cttacctccg aaaatattag cgttcacggc ttcgacaagt ccgccctttt
     2161 cgataatagc ctgaatgaag ggagggaagg cggcagcctt gtactgctta ttctttgtgt
     2221 agttgacaat gacaccgctg tcaaagtcca tgccgatttc gtcgccgttt tcagcgtcgg
     2281 cgacagcctc ggggcactct atgatgggaa gtccgata
//
LOCUS       DAONVQ010000181         2222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_32903_length_2222_cov_4.523304, whole genome shotgun sequence.
ACCESSION   DAONVQ010000181 DAONVQ010000000
VERSION     DAONVQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32903_length_2222_cov_4.523304"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PLD98_08785"
     CDS             <1..1045
                     /locus_tag="PLD98_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQA06377.1"
                     /translation="HYAISKVKAERIIAESGLKYWVSLRQSGILYAGILNNYDPIMFH
                     VPIRGVLEWATIEDSGRLLLNVCKDDVPEEFWRRFYNIGSGDEYRLTNYQFEELLLKT
                     IGLSDPKKIFEPNWFALRNFHGQWYADSDVLENYLHFRHNIPVKEYFNNLKKYVPSYT
                     KFAKLVPSALIKRFAMKPLTKKPIYGTMNWIEENNTDRITAFFGSREAWEKIGSWKDI
                     DLTPPTKKITYLDHGYDETKPDSELDIEDMKKAAEFRGGKCLSETMKKGDLFTPLKWV
                     SARGNEFEMTPNLVLKGGHWCPDELPWPWDYDTEAKINPFFAQVWYPLHDKDEHNYYT
                     EAIFKGMAGFENI"
     gene            complement(1132..>2222)
                     /locus_tag="PLD98_08790"
     CDS             complement(1132..>2222)
                     /locus_tag="PLD98_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06378.1"
                     /translation="IHATHVHSAVDIQGLGGDIATAFTTNPLRVLIGRDAFRENGKSK
                     VYMDNLVAKTVECLKAAFEDLKPGKLYYSAVNAYEYIKDKRLPIVFDPNLNTFKFVPN
                     DGSAETYIVNFTSHLTGLPQSNDIASSDYIYYLEQGLKEAYAQEDKEVNFAFLLGANL
                     AMTTEKEPLNLPSGTTPIDAVAAYGAKLAELILDGGNYWDEVEPLFNIRAQEIYLKVD
                     NGILLAAGKTGLVNNTAVRIGSGAFDVAMVSEMSYIEIGTEDFGFAIGLMPGELAPEL
                     AWGGTMKADTAWNGVDFEHEPMANEVGYRQLIICSQANDAIGYILPDNDYGPWLEDSH
                     YEETLSLGPNTGSTIVKIFAELVASTKA"
BASE COUNT          571 a          536 c          553 g          562 t
ORIGIN      
        1 ccactacgca atcagcaagg ttaaggcaga gaggattatt gccgaatccg gtcttaaata
       61 ctgggtatct ctcagacagt cgggaattct ctatgcgggc attctcaata actacgatcc
      121 gattatgttc catgtgccta taagaggcgt tcttgaatgg gcaaccatag aggattccgg
      181 cagacttctt ttaaatgtct gcaaggacga tgttcccgag gagttctggc gcagattcta
      241 caacatcggc agcggcgacg agtacagact caccaactat cagtttgagg agctgctctt
      301 aaaaaccatc ggtctttccg accccaagaa gatttttgag ccgaactggt ttgccctcag
      361 aaacttccac ggacaatggt atgcagactc cgatgtgctg gaaaactacc tgcacttccg
      421 tcacaacatc cccgtaaagg aatatttcaa taatctcaaa aagtatgttc cttcctatac
      481 aaagttcgca aagctcgttc cttccgccct cattaagaga tttgcgatga agcctcttac
      541 taagaagccc atctacggca caatgaactg gatagaggaa aacaatactg acagaatcac
      601 cgctttcttc ggctcgagag aagcttggga gaaaatcggc tcatggaagg atattgacct
      661 gacaccgccc actaaaaaga ttacatacct tgaccatggc tatgacgaaa caaagcccga
      721 ttccgagctt gacattgagg atatgaagaa ggccgcggag ttcaggggag gcaagtgcct
      781 gtctgagacc atgaaaaagg gcgatttgtt cactcctctc aagtgggtat ccgcaagggg
      841 caacgagttt gaaatgaccc cgaacctggt gctcaaaggc ggacactggt gccccgacga
      901 gctgccctgg ccgtgggact atgacaccga ggcaaaaatc aatcccttct tcgctcaggt
      961 ctggtatccc ctccatgata aggatgagca caactattac accgaggcca tattcaaagg
     1021 catggcaggc tttgaaaaca tataaaaata accccgcagt tttgcggggt ctttttttgc
     1081 ccaaattgcc tataaaaaag ccgcccactt tcgtgagcgg cattttttct tttaagcctt
     1141 tgttgaagca acaagctcag caaagatttt tacaattgtt gaacctgtgt tgggaccgag
     1201 ggagagggtc tcctcataat ggctgtcctc aagccacgga ccgtagtcgt tgtcggggag
     1261 gatatagccg atagcgtcgt tagcctgact gcagatgata agctgacgat agccaacctc
     1321 atttgccatg ggctcgtgct caaagtcaac gccgttccaa gctgtgtcgg ccttcattgt
     1381 gccgccccaa gcaagctcgg gagccaattc accgggcata agaccgattg cgaagccgaa
     1441 gtcctcggtg ccgatttcaa tgtatgacat ctcggatacc attgcgacat caaatgcgcc
     1501 ggagccgatt cttacagcgg tgttgtttac aagacctgtt ttgcctgctg caaggaggat
     1561 gccgttgtca accttgaggt agatttcctg agctctgatg ttgaacagag gctctacttc
     1621 atcccagtag ttgccgccgt caaggataag ctcagcgagc tttgcgccgt atgccgctac
     1681 ggcatcaatc ggagttgtgc cggaaggcag gttcaaaggc tctttttcgg ttgtcattgc
     1741 aaggtttgcg ccgaggagga atgcgaagtt aacttcctta tcctcctgag catatgcctc
     1801 tttcagaccc tgctcaaggt agtagatata gtctgaagac gcaatgtcat tggactgcgg
     1861 cagacctgtt agatggctgg tgaagtttac tatgtatgtt tctgccgagc cgtcgttcgg
     1921 tacgaattta aaggtgttga gattggggtc gaaaactatc gggagtctct tatccttaat
     1981 gtattcatat gcatttacag cggagtaata gagcttgccg ggctttaagt cctcgaatgc
     2041 agctttgagg cactcaacgg tcttagcaac aaggttatcc atgtaaacct ttgatttgcc
     2101 gttctctctg aaagcgtctc tgccgataag cactctgaga ggattggtgg tgaatgcggt
     2161 agcgatgtcg ccgccgagac cctgaatgtc aaccgcgctg tgaacatgag ttgcatgaat
     2221 gt
//
LOCUS       DAONVQ010000182         2156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_34223_length_2156_cov_4.842456, whole genome shotgun sequence.
ACCESSION   DAONVQ010000182 DAONVQ010000000
VERSION     DAONVQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34223_length_2156_cov_4.842456"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1896
                     /locus_tag="PLD98_08795"
     CDS             67..1896
                     /locus_tag="PLD98_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HQA06379.1"
                     /translation="MANFKLWALDTGKMSFDGRFFEALKFIEDFQLLDEKLWTVFVKL
                     FETNPDDKDLGWRSEFWGKMMRGAALVYRYTKNERLFEIMEKSVRNLLTTADSLGRIT
                     TYSIEKEFRQWDIWGRKYVLLGLEYFLEICADEKLKDGIISSMRRQADYIISKVGNSE
                     GKIPITHTSEFWEGLNSSSLLEPIVRLYRITGDEKYLDFAHEIVRCGGTKNFDIFKAA
                     LEGKLYPYQYPVTKAYEMMSCFEGILQLYMVSDNPDYLKMAENFARLVIDSDITIIGN
                     AGCTHELFDNSRARQFDPAFTGIMQETCVAVTWMKFFFQLLSLTGKALYADEIEKTAL
                     NSLLGSVNFEKNKMDGVTLPVDSYSPLLFARRGQAVGGKKDLKDINYYGCCAAINAAG
                     FGIGALFSVMSSYSGIAINMYHSGVAEFETPDGKNVILRIEGNYPTQGDIKIYIEPET
                     QSKFAVSLRIPEWSDMTSLKVCGEEARAEKGSYAVIDRLWKKGDIIELRLNMDIKVIE
                     AEDIEPNAPEAALKHFALKKGPIVLARDSALEGEDIRIPVKSENGKVKIKEIKPTKTA
                     DFKTQAEYEIVFSKGDRIRVIDYASAGGVWSEDRIISAWLNKE"
     gene            complement(1897..>2156)
                     /locus_tag="PLD98_08800"
     CDS             complement(1897..>2156)
                     /locus_tag="PLD98_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQA06380.1"
                     /translation="KETVDNGNGAAAKPDFATAAGKTATAQTGQYENGKELLVTWFAG
                     FFPAEEPEYVIVIMKERGSASTKDCTPVFKYIADMIIKNKL"
BASE COUNT          600 a          447 c          565 g          544 t
ORIGIN      
        1 caattagaga ttgacattta taggctaaac cgtaaactgt aattaacaaa agttttggag
       61 gctaatatgg cgaattttaa gctttgggct ttagatacag ggaaaatgag ttttgacggg
      121 cgcttttttg aggctttaaa gtttattgag gattttcagc tccttgacga gaagctttgg
      181 actgtttttg taaagctgtt tgagacaaat cccgacgaca aagacctcgg ctggagaagc
      241 gaattctggg gcaagatgat gcgcggcgcg gctctggttt acagatacac gaaaaatgaa
      301 aggctctttg aaattatgga aaagagcgta agaaatctgc tgactaccgc cgactccctt
      361 ggcagaatca caacttacag cattgaaaag gagttcaggc agtgggacat ttggggcaga
      421 aaatatgtgc tgttgggtct tgagtacttc cttgaaatct gcgccgatga aaagctgaaa
      481 gatggaatta taagctccat gagaaggcag gcggactata taatctccaa agtcggaaat
      541 tctgaaggta aaattccgat tacccatacc tccgagtttt gggaaggtct taattccagt
      601 tcgctgctgg agcctattgt taggctttac agaatcacag gggacgaaaa ataccttgac
      661 tttgcccatg agatagtccg gtgcggcggc actaagaatt ttgatatttt taaagccgcc
      721 ttggaaggta agctttatcc gtatcagtat cccgtcacaa aggcatatga gatgatgtcc
      781 tgctttgagg gcattttgca gctttatatg gtaagcgaca accccgatta tcttaaaatg
      841 gcggagaact tcgcaagact tgttatcgac tccgacatta cgataatagg aaacgccggc
      901 tgcacccatg agctgtttga caactcaagg gcaaggcagt tcgaccccgc ttttacgggc
      961 ataatgcagg aaacctgtgt cgcggttacc tggatgaagt tcttctttca gcttttgtcg
     1021 ctgacgggca aggcgctgta cgctgacgag attgaaaaga ctgctcttaa cagccttctt
     1081 ggcagcgtga actttgaaaa gaataagatg gatggagtaa ccctccccgt tgacagctac
     1141 tctccccttc tctttgcccg cagaggtcag gcggtaggcg gaaagaagga tcttaaggat
     1201 ataaactact acggctgctg cgccgcgatc aacgccgcgg gctttggcat aggcgcgctg
     1261 ttctccgtaa tgagttcata tagcggcata gccataaata tgtatcacag cggcgtggct
     1321 gagtttgaga cacccgacgg caaaaatgtt attttgagaa ttgagggcaa ttacccgaca
     1381 cagggagata taaagattta tattgagccc gagacccaga gcaaattcgc cgtaagtctt
     1441 agaattcccg agtggagcga catgacatcg cttaaggttt gcggcgagga agcaagagcg
     1501 gagaaaggct catacgccgt tatcgatagg ctttggaaaa agggagatat tattgagctg
     1561 aggctcaaca tggatataaa ggtcatagaa gctgaggata tagaaccgaa cgctcccgaa
     1621 gcggcgttaa agcattttgc tctgaaaaag gggccgattg tgctggcaag ggattctgct
     1681 cttgagggag aggatataag aattcccgta aaaagcgaga acggcaaagt gaaaatcaaa
     1741 gaaataaagc cgacgaaaac cgccgacttt aagacacagg ctgaatatga gattgtattc
     1801 tcaaagggcg accgcataag ggtcattgat tacgcttccg cgggcggagt ctggtctgag
     1861 gacaggataa tctccgcttg gcttaacaag gaataattac agcttattct taattatcat
     1921 atcagcaata tatttaaaca caggggtgca gtccttagtt gaggcgctgc ccctttcttt
     1981 cattatgact attacatact ctggctcctc cgcggggaaa aagccagcga accatgtgac
     2041 taaaagctcc ttgccgtttt cgtactgacc cgtctgggca gtggcggttt tgcccgccgc
     2101 ggtggcaaag tcgggctttg cggcggcgcc gttgccgttg tcgacggttt ccttga
//
LOCUS       DAONVQ010000183         2125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_34911_length_2125_cov_4.652174, whole genome shotgun sequence.
ACCESSION   DAONVQ010000183 DAONVQ010000000
VERSION     DAONVQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34911_length_2125_cov_4.652174"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="PLD98_08805"
     CDS             complement(<1..1095)
                     /locus_tag="PLD98_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQA06381.1"
                     /translation="MLKFNENGKFTIMVATDIHEKADVTSPLAKKKMRDGRLLMETAL
                     DKIKPDLVVLLGDIFTNREKEEPYADFRAAIRRVAQPMIDRGIPYAIALGNHDHDGYH
                     TDKQVEMYKELEGCLIDNADPNITGNANYNLLIYSSKGDKPVFNLWFIDSNNHYEDIS
                     VSKYDIVHQDQIEWYEKTALALKEQNGGKVIPALVFQHIPVIQCYRLLRKAKLWERPW
                     AVRGEGAFQKHYYVLKDGVEGYLGQGPCTPDIDSGQFDSWKKIGDVIGAYFGHDHMND
                     FNGQVDGIYLGQCKTASFRVYTDGARSGVREIIVNENDPTKYETKMYHFKEELGLEPI
                     SLGPIMKRLTDRQSVNLTIARNVLLGAGAAY"
     gene            1347..>2125
                     /locus_tag="PLD98_08810"
     CDS             1347..>2125
                     /locus_tag="PLD98_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06382.1"
                     /translation="MASYCPKCNYKLRLRDYKPECPQCGVNILYYGMEERLAREADAA
                     EKEHAEFQPKIDRVKAAVYGSKVAIARIPIILLPILSLLLPLGKVSMMLPFYEEAKNT
                     TVNLISIVKLLSNLNFDLVLKMVNSPIFGKVFVFFLASIVCLILSLLVMLTNLIVLPL
                     SCSPKGLPRNFTLAGMGIVFSSLSAVFFHLMGKEITAVVPEGVYSSSLGFGIAFVLLS
                     FVAIIVVNALFKILKIEVKYTDVSEYLKPFEERQREKAEKEA"
BASE COUNT          531 a          493 c          473 g          628 t
ORIGIN      
        1 ataagcggct ccggctccga gaaggacatt gcgggcaatt gtaaggttta cgctctgcct
       61 gtcggttaac ctcttcatga tgggacccaa agagatgggt tcgagcccta actcttcctt
      121 aaagtgatac atctttgttt cgtactttgt ggggtcgttc tcgttgacga taatctcgcg
      181 aacgcctgag cgggcgccgt cggtgtaaac tctgaagctg gcggtcttac actgacccag
      241 ataaattccg tcaacctgac cgttaaagtc gttcatgtgg tcgtgaccga aatatgcgcc
      301 gattacatcg ccaatcttct tccagctgtc aaactgaccg ctgtcaatgt cgggtgtgca
      361 gggaccctgt ccgagatagc cctcaacgcc gtctttaaga acatagtagt gcttttggaa
      421 agctccctcg cccctaacag cccaaggtct ctcccaaagc ttagcctttc ttaaaagcct
      481 gtagcactga ataactggga tgtgctggaa cacaagggcg gggatgacct ttccgccgtt
      541 ctgctcttta agagcaagag ctgttttctc ataccattct atttggtcct gatggacaat
      601 atcgtatttt gaaacgctaa tgtcctcata atgattgttt gaatcaatga accaaaggtt
      661 gaatacaggc ttgtcgcctt tactggaata tataagaagg ttatagttcg cattgcctgt
      721 tatgttcggg tcggcgttgt ctataaggca gccttcaagc tccttataca tctcaacctg
      781 cttgtcggtg tggtaaccgt cgtggtcgtg atttcccaaa gcaatcgcat agggtatgcc
      841 cctgtcaatc atgggctgag cgactctcct gatggcagct ctaaagtctg cgtagggctc
      901 ttccttttcc ctgtttgtaa agatgtcgcc aaggagaacc acaaggtccg gcttgatttt
      961 atcaagagcc gtttccatga gcagcctgcc gtctctcatc tttttcttag caaggggaga
     1021 ggtgacatcc gctttttcat gaatatcggt cgcaaccatt attgtaaact tgccgttttc
     1081 attaaattta agcatcgcta acctccggtc ttttaatgtc tactattatc cttcaatttc
     1141 acacttctgt caaggttcga cggcaaaaaa tgaagcaact gaaaaaagct cggcatagtt
     1201 ttcatttgtc aaaaaatgca aaacattgaa agcagaaaat actattgctt tttatggtgt
     1261 aatatgataa attgttttta gttattcggc gcttatttag cttaattgct aattttgccg
     1321 aagcattaca taacggaggt atacatatgg cgtcatattg ccccaaatgc aattacaagc
     1381 tgcgtcttag agactataaa cccgaatgtc cacagtgtgg tgtaaatatt ctgtactatg
     1441 gcatggagga aagattggca agagaggctg acgctgccga gaaggagcat gccgaattcc
     1501 agcccaagat tgacagagtt aaggcagcgg tttacggctc aaaggttgcg attgcaagaa
     1561 taccgataat tctccttccg atattgtccc tccttttgcc cctaggcaag gtttcaatga
     1621 tgcttccctt ctatgaagag gctaaaaaca caaccgttaa ccttatttca atagtaaagc
     1681 ttctttcaaa cttaaacttt gaccttgtgc tcaagatggt caattccccg atattcggca
     1741 aggtgtttgt gttcttcctg gcatcaatcg tttgcctgat actttcgctc cttgtcatgc
     1801 tcacaaacct cattgtgctt ccgctttcct gctcacccaa gggactgccc agaaacttta
     1861 cccttgccgg catgggcata gtattctcaa gcctttcggc tgttttcttc cacctgatgg
     1921 gcaaagagat tacagcggtt gttcccgaag gcgtatacag ttcatccctc ggcttcggta
     1981 ttgcgttcgt actgctttca ttcgttgcga ttatcgtagt aaacgcgctg tttaagatac
     2041 ttaagattga agtaaagtat accgatgtct ccgagtattt aaagcccttt gaggagaggc
     2101 agagggaaaa ggctgaaaag gaagc
//
LOCUS       DAONVQ010000184         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_36968_length_2035_cov_3.650000, whole genome shotgun sequence.
ACCESSION   DAONVQ010000184 DAONVQ010000000
VERSION     DAONVQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36968_length_2035_cov_3.650000"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..1010
                     /locus_tag="PLD98_08815"
     CDS             387..1010
                     /locus_tag="PLD98_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQA06383.1"
                     /translation="MMKNIIFDIDGTLIDTEYSILTSLQKVLLEEEGRQCSLEDLRFC
                     LGIPGTSVMARLKPKDPQRLQYRWNQEMNKHTDSIKPFDGILEVLETLKEKGIKMGIV
                     TSKTQEEFDTDFKPFGINHYFDSIINANHTEKHKPHPEPLLKCMEEMGAAKEETVYIG
                     DTVYDMDCAKGADVKFGLALWGSKTTEGFDKADFILKETREILNLIK"
     gene            complement(1081..>2035)
                     /locus_tag="PLD98_08820"
     CDS             complement(1081..>2035)
                     /locus_tag="PLD98_08820"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQA06384.1"
                     /translation="FVSKVFKPFVKILVLSTNGIVRLFGIDPNDNEDTANEEEIRMMV
                     DQGEKKGFIENTQREMIINVFEFDDIDAGDIMTHRVDMVAVEADEPIDEVIKAAIEEG
                     YSRIPVYDDEPDNIIGIVYVKDLLQYVGTEIPKTKTIREIMREAYLIPESKKCGELFT
                     EMTEKRVQMAIVVDEYGGTAGLVTMEDLLEAIVGNIRDEYDTEEEEDINKINDTTFTI
                     DGITDIDELGELTGTHLPEGDYDTVGGFIISRLGYLPADGDMDVTEYKNLRFTVLSVE
                     DNKRIDKVKVEILPEPVTDENGDKLTETAEIKENGKKKKKQ"
BASE COUNT          484 a          534 c          491 g          526 t
ORIGIN      
        1 ccccgccgcc cgcaccgtcg ggtgggggga gcggtctgcc catctgtgaa accctgccgc
       61 ccgccgatga gcggggagcg gcagttctcc gccgtcagag cgggtcagta tcccgccaca
      121 ccatgtccga cagtcggtgc tctcttgtgg cggcggcgac cccacgcccc accgccccgc
      181 accgtatggt gggggagcgg gctgcccatc tgtgaaaccc cgccgccgcg ccccgaaagg
      241 cggctgagat tcgtcaccct gcggtcaatt tgcgggggtg tttgtttaat ataggcgaaa
      301 gttctctgct tttctctctg atttttggca agcgttaagt cgaaatatgg ggtataatta
      361 aatcagagag ataaaggaga atgcctatga tgaaaaacat aatttttgat atagacggta
      421 cacttatcga cacggaatac tccatactca catcccttca aaaagtgctc ttggaagaag
      481 agggcaggca gtgctcactt gaggatttaa gattctgcct cggaattccc ggcacctccg
      541 ttatggcaag gctgaagccg aaggacccgc agagactcca gtacagatgg aatcaggaga
      601 tgaacaagca caccgattcc ataaagccct ttgacggtat tttggaggtc ctcgaaacgc
      661 tcaaagaaaa gggcattaag atgggcatag taacatccaa aactcaggag gagtttgaca
      721 cagactttaa accctttgga atcaatcact atttcgactc cataatcaac gccaatcaca
      781 ccgaaaagca caagccccac ccggagcctt tgctaaagtg catggaggag atgggcgccg
      841 ccaaagaaga aaccgtgtat atcggcgaca ccgtttacga catggactgc gcaaagggcg
      901 cggatgtaaa gttcggtctc gctctttggg gctcaaagac aaccgagggc tttgacaaag
      961 cggactttat tcttaaagaa acaagggaaa tcctaaatct tataaaataa aaaaaaggct
     1021 gagtttcagc cctataaaaa ctaagggagc tttaaaaagc tcccttttat tataacagtg
     1081 ttactgtttt ttctttttct ttccgttttc cttaatctcc gccgtttctg tcagcttatc
     1141 tccgttttcg tccgtgacag gctcgggcag aatctcaacc ttaaccttgt cgattctctt
     1201 gttgtcctca acgctgagaa ccgtaaagcg caggtttttg tattcggtca catccatgtc
     1261 gccgtccgcg ggcagatagc cgagcctgct tataataaaa ccgccgaccg tatcgtagtc
     1321 gccctcgggc agatgggtgc ctgtcagctc gcccagctcg tcaatgtccg taatgccgtc
     1381 tatggtaaag gtggtgtcgt taatcttgtt tatatcctct tcttcttcgg tgtcgtactc
     1441 gtcgcggatg ttgcccacga tagcttcaag caggtcctcc atggttacaa gacccgcggt
     1501 gccgccgtac tcgtcgacga caatcgccat ctggacacgc ttttccgtca tctctgtgaa
     1561 aagctcaccg cattttttgg attcggggat aagatacgcc tcgcgcatta tctccctaat
     1621 ggtctttgtc ttgggtattt cggttccgac atactgaaga aggtccttta catagactat
     1681 gccgatgatg ttatcgggct cgtcgtcgta aacggggatt ctggaatagc cttcctctat
     1741 cgcggcttta ataacctcat caatcggttc gtcagcctcg accgccacca tgtcgactct
     1801 gtgggtcatg atgtcgcccg cgtcgatgtc gtcaaactca aagacattta taatcatctc
     1861 acgctgggtg ttctcgataa agcccttttt ctcgccctgg tccaccatca tgcggatttc
     1921 ttcttcgttt gccgtatcct cgttgtcgtt cgggtcaatg ccgaaaagcc ttacaatgcc
     1981 gtttgtggaa agcacgagga tttttacaaa tggcttaaat actttggaaa cgaat
//
LOCUS       DAONVQ010000185         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_39241_length_1944_cov_3.844362, whole genome shotgun sequence.
ACCESSION   DAONVQ010000185 DAONVQ010000000
VERSION     DAONVQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39241_length_1944_cov_3.844362"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLD98_08825"
     CDS             complement(<1..445)
                     /locus_tag="PLD98_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HQA06385.1"
                     /translation="MKYYLENVSEVLRKVSATENGLSPQEAAKRLEQNGRNKLKEAEK
                     ESLIKRFFDQLKDPMLIILIIASIVSAVTTFISNRTSGHSEGYADFFIIMIVVLINAV
                     LGVYQESKAEKAIEALQKLSAATSKVLRDGKIVTIPSEELVVGDVV"
     gene            complement(795..1109)
                     /gene="trxA"
                     /locus_tag="PLD98_08830"
     CDS             complement(795..1109)
                     /gene="trxA"
                     /locus_tag="PLD98_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQA06386.1"
                     /translation="MSVIKITKENFNSEVLKSDVPVLIDFWAPWCGPCRALSPLIDEI
                     AEEANGKFKVGKVNVDEQPELASKFSVMSIPTLVVMKNGELVNKTVGSRPKEQILSLI
                     EG"
     gene            1326..>1944
                     /locus_tag="PLD98_08835"
     CDS             1326..>1944
                     /locus_tag="PLD98_08835"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQA06387.1"
                     /translation="MDDIIIVGSGPAGISASLYIKRAGLSVKLIGKDGGSLEKADKIQ
                     NYFGLEKAMSGSELIQIGLRQAKELGVEHITEEVLDISYADEGFEVAVKNGSYKALAV
                     LLATGAARKAPRIEGLRQFEGMGVSYCAVCDGFFYRGKEVAVLGHSEYALHEVKELLP
                     IASKVSVLTNGMAPDAEFPENVKVYTSKIKRLFGSETLEGAEFADG"
BASE COUNT          544 a          421 c          436 g          543 t
ORIGIN      
        1 gaacaacatc gccgacaaca agctcctcgc tgggtatggt gactattttg ccgtctctaa
       61 gtacctttga ggtagcggct gacagcttct gcagtgcctc aatggccttt tcagccttgc
      121 tttcctgata cacaccgaga acagcgttga taagcacaac aatcataatg atgaaaaaat
      181 ccgcatatcc ttcgctgtgc ccggaagttc ggttggaaat gaaggtggta accgctgaaa
      241 cgattgaagc gatgatgaga ataatcagca tggggtcctt aagctggtcg aagaatctct
      301 taatcaagga ctccttctct gcttccttga gcttattcct gccattctgc tcaagtcgtt
      361 ttgcggcctc ttgggggcta aggccgtttt cggttgcgga aacttttcgc aatacttctg
      421 agacattctc aaggtagtac ttcatgcttt atttcctttc aatgtaattt tcagccgctt
      481 ttggggccat caaaataaag tatattgctt ttccatcggg aaaatacaag ccggcgtatt
      541 ataaaaaaac tcatgtatag tcagggctac caactataca tgagtctggt catttaattc
      601 aagccaagcc cttcggctat gatgttgact tgacacgcat tatataatgc gccaactact
      661 ccctcatggt tgctatatac ttttggaata ttatatatat catccaataa tatgtcaata
      721 gataaaaaca aaaatagtta aaaaatagac aaaaaaatag ccgggaaccc ttcccggcta
      781 taaggtttat gctgttaacc ctctataagg cttaaaatct gctccttagg tcttgagccg
      841 acggttttgt ttacaagctc gccgttcttc ataacaacca gagtcggtat gctcattact
      901 gagaactttg aggcaagctc gggctgctca tccacattta cttttccaac cttaaatttt
      961 ccgtttgcct cctcggcaat ctcgtcaatc agaggggaga gcgcacggca gggaccgcac
     1021 cagggagccc aaaaatctat aagaacggga acatcggact tgagtacctc ggaattgaag
     1081 ttttcttttg ttatttttat aactgacata ttatttcctc ccaaaaaact cataatctat
     1141 tatatgatta taaatacttc cataatttat tctgtaacaa ggttacatta atattccgtt
     1201 tttgttatta tacttgtaaa aaaatagaag ctggggtaaa atgaccttag atacatatta
     1261 cattaatttt ccgatttatt caatataagt ttgttttttc gagcttggaa acggagatgt
     1321 gaaacatgga cgatattatt attgtcggaa gcggccccgc gggaatctcc gcctctttgt
     1381 atataaagcg cgccgggctg tcggtaaagc tcataggcaa agacggcggc tctcttgaaa
     1441 aggccgataa aatacagaat tatttcggac ttgaaaaggc aatgtcaggc tccgagctga
     1501 tacagattgg cctcagacag gcaaaggagc tgggagtgga gcacattacc gaggaagttc
     1561 tggatatatc ctacgccgat gagggttttg aagtagccgt aaagaacggc agctacaagg
     1621 ctttggcggt tcttttggca acgggcgcgg caagaaaggc gccgagaatc gagggactgc
     1681 ggcagtttga aggcatgggg gtcagctact gcgccgtctg cgacggtttc ttctacaggg
     1741 gcaaggaggt tgctgttttg ggtcacagcg aatacgccct ccacgaggta aaagagcttc
     1801 tgcccatcgc ctcaaaggtt tcggtattaa caaacggcat ggcgcccgac gcagaattcc
     1861 ccgaaaatgt aaaagtatat acttcaaaaa taaaaaggct tttcggctct gagacccttg
     1921 aaggcgctga gtttgccgac ggcg
//
LOCUS       DAONVQ010000186         1885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_40877_length_1885_cov_3.709290, whole genome shotgun sequence.
ACCESSION   DAONVQ010000186 DAONVQ010000000
VERSION     DAONVQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40877_length_1885_cov_3.709290"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="PLD98_08840"
     CDS             <1..1318
                     /locus_tag="PLD98_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQA06388.1"
                     /translation="PMEKIESAESAFEQAAIMWNENTDTAATLKAFAQSREEFKGRVE
                     AINRLITRGSISFADKESAKKLYGEKMFISPSKVETFYRCPFMFFCRYGVRAKPLKAA
                     VFDFSKSGTAVHYSLEKLISEQGIEKLSALPAEELKALIHSAVTEYSDRYLGSMHESS
                     ARNRYVLEKQAETVYEVLIRLIEEFKLSRFRIKDVELKMSYDGEVRPYELILPDGSAV
                     AITGTVDRVDIMQESGRSYIRVMDYKTGGVAKGRFKIKDILNGLSMQMLIYLIGLCEN
                     GGARYGDELLPAGVLYVPARAEVIKEGRHLSEDEEREKKLKLGKMKGLILDLPEVIMG
                     MDSSGTGIFIPAFIDASGFVKGSVYSLQQFKALKEYIDKKIKDMATALREGNVSALPT
                     ENKVCESCDYNAVCGREKDGPVRQLYDLDDDAAWQEIFNAAGMNEE"
     gene            1348..>1885
                     /locus_tag="PLD98_08845"
     CDS             1348..>1885
                     /locus_tag="PLD98_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQA06389.1"
                     /translation="MSKVNWTDQQKRAIELRGGSIALSAAAGSGKTAVLVERVIEILL
                     DDAEPVDPSELLVVTFTNAAANEMRQKIKRAINEKIAENPFSSRLNRLKIGIESANIC
                     TMDSFCIKLLRENFHLAGVSPDFRPLDGSEEESLKKDILDMVLEELYVERGEPFRRMA
                     DILSDGRDDSSLGEFVSRL"
BASE COUNT          518 a          468 c          522 g          377 t
ORIGIN      
        1 gcctatggaa aaaatagaga gcgccgagtc ggcctttgag caggcggcga taatgtggaa
       61 cgaaaacacc gacacagcgg cgaccctcaa agcgttcgcc caaagccgcg aggagtttaa
      121 gggcagggtg gaggctatta acagacttat aacaaggggc agcatctcct ttgccgacaa
      181 ggagagcgcg aaaaagctct acggcgaaaa gatgtttata agcccttcaa aggtagagac
      241 cttctaccgc tgccctttta tgttcttctg ccgctacggc gtcagggcaa agcccttaaa
      301 ggccgccgtt tttgactttt caaaaagcgg caccgccgtg cactattctc ttgaaaagct
      361 tatctccgag cagggcatag aaaagctgag cgccctgccc gctgaggagc ttaaagccct
      421 tatacactcc gccgtgaccg agtattccga cagatacctc ggctcaatgc acgaaagctc
      481 cgcccgcaac cgatatgttc tggaaaaaca ggcggaaacc gtatacgagg tgctcatccg
      541 ccttatcgag gagtttaagc tgagcagatt caggattaaa gatgtggagc ttaaaatgtc
      601 ctacgacgga gaggtaagac cctatgagct gattctcccc gacggctccg ccgttgccat
      661 caccggcacc gttgacaggg tggacattat gcaggagtcg ggcaggtcat atatcagagt
      721 catggactac aagacaggcg gcgtggcaaa gggcagattt aagataaaag acattcttaa
      781 cggacttagc atgcagatgc tcatatacct tataggtctt tgcgaaaacg ggggagcgag
      841 gtacggcgat gagcttctcc ccgcgggcgt gctctatgtc cccgcaaggg ctgaggtaat
      901 taaagaaggc agacatcttt ccgaggacga ggaaagggag aaaaaactca aactcggcaa
      961 gatgaagggt ctaatccttg atttgcccga ggtaataatg ggcatggact caagcggcac
     1021 gggaattttc atccccgcct ttattgacgc ctcaggcttt gtcaaaggct cggtatattc
     1081 cctccagcag tttaaggctc tgaaggagta catagacaag aaaatcaagg acatggcgac
     1141 agctctcagg gagggaaatg tttcggctct gcccacggaa aacaaagtct gcgagagctg
     1201 cgactacaac gcggtttgcg gcagggaaaa ggacggcccc gtaagacagc tttatgacct
     1261 tgacgacgac gctgcatggc aggaaatctt taatgcggcg ggcatgaatg aggaataaaa
     1321 attagcggcg cagtttagga ggaaactatg tctaaggtga actggacaga ccagcagaaa
     1381 agagccattg agctcagagg cggctccata gccctttccg cggcggcggg ctcaggcaaa
     1441 accgccgttc tggtagaaag ggttattgag attcttttag acgacgcgga gccggtcgac
     1501 ccctccgagc ttttggtggt cacctttaca aacgccgccg ccaacgaaat gcggcagaaa
     1561 ataaaaaggg cgataaacga gaaaatagcc gaaaacccct tcagctcccg cttaaatcgc
     1621 ctcaaaatcg gcatcgaaag cgcgaacatc tgcaccatgg actccttttg cataaagctt
     1681 ctcagagaaa acttccacct tgcgggcgtc tcccccgact tcagacccct tgacggttct
     1741 gaggaggaga gcttaaaaaa ggacatcctc gacatggtgc tggaggagct gtatgtggaa
     1801 aggggcgagc ctttcagacg catggcggac atcctcagcg acggcaggga cgacagcagt
     1861 ctgggcgagt ttgtttcaag gctct
//
LOCUS       DAONVQ010000187         1857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_41684_length_1857_cov_4.854606, whole genome shotgun sequence.
ACCESSION   DAONVQ010000187 DAONVQ010000000
VERSION     DAONVQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41684_length_1857_cov_4.854606"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLD98_08850"
     CDS             complement(<1..629)
                     /locus_tag="PLD98_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06390.1"
                     /translation="MIDFKDTVFFLGGNTPKGFYAFLTELYNPFDKWRMYIIKGGPGT
                     GKSYFMKKIARELESLGVSVERILCASDPKSLDAIICHELKLTIADGTPPHVLEPRFP
                     GVVEQILNFGDFWDETLLREKGMDIRSAALMNSEEHKNCARILKAAGALEEESRRLTE
                     DIVLKDKVKRFSQRLAAREFVRKKGVPSREYLRFLSAITPEGITFLHETV"
     gene            797..1456
                     /gene="nth"
                     /locus_tag="PLD98_08855"
     CDS             797..1456
                     /gene="nth"
                     /locus_tag="PLD98_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQA06391.1"
                     /translation="MKKAKDGLTDKKRAELAIDALKKEYPEGICSLIADEPFQLLVAT
                     RLSAQCTDARVNMITPALFAKYPTIEDFANADVADVEQLIHSCGFFRQKAKDIVGMAN
                     MILSEYGGKVPDTIEELTKLPGVGRKTANLIIGDVYKKPAVVADTHLIRISNRLGLVS
                     VKDPYKVEMKLKEILDPAESNDFCHRCVLHGRAVCAARKPRCEKCVMKDFCLYYFEQN
                     S"
     gene            1477..>1857
                     /locus_tag="PLD98_08860"
     CDS             1477..>1857
                     /locus_tag="PLD98_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQA06392.1"
                     /translation="MSAPLAERMRPERIEDMIGQRHLLSEGAALRNVLESGECPNLIF
                     YGPPGTGKTTLAGIIAKNSEKRLHKLNGTSMSIADIKDVIASLDSLSAANGVLLYIDE
                     IQYLNKKQQQVLLQHIENGDITLIA"
BASE COUNT          495 a          469 c          439 g          454 t
ORIGIN      
        1 accgtttcat gtaggaaggt aatgccctcg ggcgtgatgg cgcttaaaaa cctcagatac
       61 tcccttgagg ggacgccctt tttccttaca aactcccttg ccgcaagacg ctgtgaaaaa
      121 cgctttacct tatctttaag cactatgtcc tcggtaagtc tcctgctttc ctcctccagc
      181 gctcccgcag cctttaagat tctcgcgcag tttttgtgct cctccgagtt catgagggcc
      241 gcgcttctga tatccattcc tttttctcta aggagcgttt catcccaaaa gtcgccgaaa
      301 ttaagtatct gctccacaac gccggggaac cttggctcca gcacatgagg aggggtgccg
      361 tcggcgattg tgagcttcag ttcatggcag ataatagcgt caaggctctt cgggtcagag
      421 gcgcagagta tcctctccac cgaaacgccc agactttcaa gctcccttgc tatctttttc
      481 ataaaatagg attttcccgt tcccggaccg cccttaatta tatacatcct ccatttgtca
      541 aaggggttat acagttccgt taagaacgca taaaacccct tcggtgtgtt tcctcccaga
      601 aaaaacacag tatctttaaa gtcaatcata aatatcctcc cgcatatttt gccgacaaaa
      661 tgttgctcta actttatgtt attcaaatgc ggcttgacag gagactttaa gctgagattt
      721 ttttacagcg gcgctttata gtcaagccct ttcgactgtg gtataatggt gtaaagaatt
      781 ttttgcagag gtacatatga aaaaagcaaa ggacggctta acggataaaa aacgggcaga
      841 gctggctata gatgctctaa aaaaagaata ccccgaggga atttgctcgc taatagcgga
      901 tgagcccttt cagcttcttg ttgccacaag gctctcagcc cagtgcacag acgccagagt
      961 caacatgata acccccgccc tctttgcaaa ataccccacg attgaggact ttgcgaatgc
     1021 ggatgtggcc gatgttgaac agctcataca ctcctgcgga tttttcaggc agaaggcaaa
     1081 ggacattgtg ggcatggcga atatgattct gagcgaatac ggcggaaaag tgcccgacac
     1141 catagaggaa ttaacaaaac tccccggcgt gggcagaaaa acagcgaacc tcatcatagg
     1201 cgatgtctac aaaaagcccg ctgtggtggc ggacacccat ttaatcagga tttcaaacag
     1261 gctggggctg gtttccgtca aggaccctta taaggtggaa atgaaactta aagagattct
     1321 tgaccccgcc gaaagcaacg atttttgcca cagatgcgtt ctccacggca gggcggtttg
     1381 cgccgccaga aaaccccggt gcgagaaatg cgtcatgaag gatttttgcc tatactattt
     1441 tgagcaaaac agttaaggca ggaagggagg aagtttatgt ctgcgccctt ggctgagcga
     1501 atgagacccg aaagaattga agatatgata ggtcagcggc atctcctctc ggaaggcgcc
     1561 gctttaagga atgtccttga gtcgggggag tgcccgaacc tgattttcta cggccctccg
     1621 ggaacgggca agaccaccct tgcgggcata atcgctaaaa acagcgagaa aaggctccac
     1681 aagctcaacg gcaccagtat gtccatcgcc gacattaagg atgtcatagc gagccttgac
     1741 tccctttccg ccgcgaacgg cgtgctttta tacattgacg agattcagta ccttaataaa
     1801 aagcagcagc aggtgctttt gcagcatata gaaaacggcg acatcaccct gattgcc
//
LOCUS       DAONVQ010000188         1766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_44536_length_1766_cov_4.112800, whole genome shotgun sequence.
ACCESSION   DAONVQ010000188 DAONVQ010000000
VERSION     DAONVQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44536_length_1766_cov_4.112800"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="PLD98_08865"
     CDS             complement(<1..1593)
                     /locus_tag="PLD98_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQA06393.1"
                     /translation="MTLFEKLNKDVIFFDGGLGSLLIAKGVTTDEPPEFLNITAPEAL
                     IDIHLSYLNAGSDIITTNTFGANAIKLHSDMYTLSEVITAAIKNAKSAVEKSGKNALV
                     AFGMGPTGKLLEPMGELKFDDAVATYTEAASIAEKAGADLIIIETMTDTYEMKAAVLG
                     AKEASDLPIIATFSFDKDGRLLTGGNIKAGITLLEGLKVDALGINCGFGPDVVKRLLP
                     EFTEFATTPIIVQPNAGLPVTEGGKTRYNISPVEFAEFQAEFLKEGVRILGGCCGTTP
                     EHIKALVKACEGIEPKKLSNRHRTVVSSYAKAQEIDDIPLIIGERINPTGKKLLKQAL
                     KEKNLDYIVSEGISQKENGAHILDVNVGMPGIDEEEMMLLAIKALQSSNPLPLQIDTS
                     NPAVMEKVMRYYNGKPLVNSVNGSEQSMHSIFPLVVKYGGVVIALTLDDNGIPETAEG
                     RLEIAKRIAENAKSYGIPLSDIIFDPLAMAVSSDKNAARTTLKAMELIKKELGAKVSL
                     GVSNISFGLPSREVITSAFFAAA"
     gene            complement(1609..>1766)
                     /locus_tag="PLD98_08870"
     CDS             complement(1609..>1766)
                     /locus_tag="PLD98_08870"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQA06394.1"
                     /translation="PEAMKQAGIAYAVNQIIDLIANGVKGIHIYTMNKPDIAEEIMRS
                     ISSIIKG"
BASE COUNT          411 a          422 c          406 g          527 t
ORIGIN      
        1 agcggcggca aagaaagcgg aagttataac ttctctgctc ggaagaccga atgaaatatt
       61 tgaaaccccc aaagaaacct ttgcgccaag ttccttcttt ataagctcca ttgcctttaa
      121 agttgtgcgg gcggcgtttt tgtcggagct taccgccata gcaagggggt caaatataat
      181 atcgctcaaa ggtatgccgt agctttttgc gttttcggct atcctttttg caatttcaag
      241 gcgaccctct gcggtttcgg gaattccgtt gtcgtcaagg gtgagagcga taacaactcc
      301 gccgtatttg accacaaggg ggaaaatgct gtgcattgat tgctcagagc cgtttacgga
      361 gttaacgagt ggcttgccgt tatagtacct cattaccttt tccattacgg cggggtttga
      421 ggtgtcaatc tgcaggggaa ggggatttga gctttgcagt gcctttatgg caagcagcat
      481 catttcttcc tcatcaatgc ccggcatacc cacattcaca tcgagtatat gggcgccgtt
      541 ttctttctga gaaatgccct ctgagactat ataatcaaga ttcttttctt ttaaagcctg
      601 ctttaaaagt tttttgccag tgggatttat tctctcgccg attataagcg gtatgtcgtc
      661 aatctcctga gcctttgcgt atgaagagac cactgttctg tgtctgttgc tgagcttttt
      721 aggctctatt ccctcgcaag cctttacaag agccttaata tgctcgggcg tggtgccgca
      781 gcatccgccg aggattctta cgccttcctt taaaaactca gcctgaaact cagcgaactc
      841 cacggggctt atgttatatc ttgttttgcc tccttctgta acaggcaaac ctgcgttggg
      901 ctgaacaatt atcggagtag tcgcaaattc ggtaaactcg ggcaaaagcc ttttgaccac
      961 atcgggacca aagccgcagt taatgccgag ggcgtcaacc tttaagcctt caaggagtgt
     1021 aatgcccgcc ttaatgttgc cgcctgtgag cagtctgccg tctttatcaa aggagaaggt
     1081 agctataatg ggaaggtcgg aagcttcctt tgcgcccaag accgcagcct tcatctcata
     1141 ggtatctgtc atggtctcaa ttataattaa atccgcccct gctttttcgg cgatagaggc
     1201 agcctctgta taagtggcaa ctgcgtcatc gaatttcagc tctcccatgg gctccaacag
     1261 cttgccagta ggtcccattc cgaaagcaac gagcgcgttt ttgccgcttt tctctacagc
     1321 gcttttcgcg ttttttatgg cggctgtaat tacttcactc agagtataca tgtccgaatg
     1381 cagcttaatc gcatttgctc cgaaggtatt ggttgtaata atgtcggagc cggcgttcag
     1441 atagctcaaa tgaatgtcga ttaacgcttc aggcgcggta atattcaaaa actcgggcgg
     1501 ttcgtcggtg gtaacgccct ttgctataag gagagaaccc agaccaccgt caaaaaatat
     1561 tacatccttg ttaagctttt caaataaagt cataatcttc tcctattttt aacccttgat
     1621 tatgctcgaa atgcttctca ttatttcctc ggcaatatcg ggcttgttca ttgtgtaaat
     1681 gtgaatgccc tttactccgt ttgcaataag gtcgattatc tgatttacgg cgtaggcgat
     1741 tccggcctgc ttcatagcct cgggct
//
LOCUS       DAONVQ010000189         1745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_45252_length_1745_cov_4.099408, whole genome shotgun sequence.
ACCESSION   DAONVQ010000189 DAONVQ010000000
VERSION     DAONVQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45252_length_1745_cov_4.099408"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /gene="dnaK"
                     /locus_tag="PLD98_08875"
     CDS             <1..1341
                     /gene="dnaK"
                     /locus_tag="PLD98_08875"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQA06395.1"
                     /translation="MVYDLGGGTFDVSIIEMGEGVQEVLATAGNNRLGGDDFDQRIID
                     WLIEEFKKDQGIDLRGDKMALQRLKEAAEKAKIELSSVTSTNISLPYITADASGPKHL
                     EMTLTRAKFNELTADLVEATMGPVRQAIADSGLKVSDIDKVLLVGGCTRIPAVQEAIK
                     AFTGKEPFKGINPDECVAIGAAIQAGVLGGEVKGLLLLDVTPLSLGIETLGGVCTKII
                     ERNTTIPTRKSQIFSTAADNQTSVEVHVLQGEREFAKDNKTLGRFHLDGIMPAPRGVP
                     QIEVTFDIDANGIVHVSAKDLATNKEQSISITASTNMSKEDIERAIKEAERYAEEDKK
                     RREETEIRNGADQMVYQSEKLLKDNGDKFTEDEKKQISEKIDALREALKGEDINLIKN
                     RQDELTKKFYELSERLYKEAAAQAQQQQQGNNTGSTQSTDNYQETPYTDVDNNE"
     gene            1494..>1745
                     /locus_tag="PLD98_08880"
     CDS             1494..>1745
                     /locus_tag="PLD98_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HQA06396.1"
                     /translation="MAEKRDYYEVLGVDRNATEDEIKKAYRRLAKQYHPDLNPGDKEA
                     EAKFKEANEAYEVLSDSEKRAKYDRFGHAGVDPNYGAGQG"
BASE COUNT          522 a          443 c          451 g          329 t
ORIGIN      
        1 atggtttacg accttggcgg cggcaccttc gatgtatcta tcattgagat gggcgaaggc
       61 gttcaggaag ttctcgcaac cgccggcaac aaccgcctcg gcggcgacga cttcgaccag
      121 agaatcattg actggcttat tgaggagttt aagaaggacc agggcattga cctcagaggc
      181 gacaagatgg ctctccagag gctcaaagag gcggctgaga aggcaaaaat cgagctttca
      241 agcgtaacca gcacaaacat cagcctgccc tatatcacag cagacgcctc cggacccaag
      301 caccttgaga tgacccttac aagggcaaag ttcaacgagc tcacagcaga ccttgttgag
      361 gctacaatgg gccccgtccg tcaggctatc gcagactccg gacttaaggt ttcggacatt
      421 gacaaggttc ttttggtagg cggctgcaca agaattcccg ccgttcagga agctatcaag
      481 gcatttaccg gcaaggagcc ttttaagggc atcaaccccg atgagtgcgt tgctatcggc
      541 gccgctattc aggcaggcgt tctcggcggc gaggttaagg gacttctcct tctcgatgtt
      601 actcccctgt ccctcggcat tgagaccctc ggcggcgtat gcacaaagat aatcgagagg
      661 aacaccacta tccccacaag aaagagccag attttctcca cagccgcgga caatcagacc
      721 tccgttgagg ttcatgttct ccagggtgag cgtgaattcg caaaggacaa caagaccctc
      781 ggcagattcc atcttgacgg cattatgccc gctcccagag gcgttccgca gattgaggtt
      841 accttcgata ttgacgcaaa cggcattgtt catgtatccg ctaaggacct tgccacaaac
      901 aaggagcagt caatctccat cacagcctcc accaatatgt ccaaggagga cattgaaagg
      961 gctataaagg aagccgaaag gtacgccgag gaggacaaga agcgccgcga ggaaacagaa
     1021 atcagaaacg gcgccgacca gatggtctac cagtccgaaa agctgcttaa ggataacggc
     1081 gacaagttca ccgaggacga aaagaagcag ataagcgaaa agattgacgc tctgagagaa
     1141 gccttaaagg gcgaggacat taacctcata aagaaccgtc aggatgagct cacaaagaag
     1201 ttctacgagc tctccgaaag gctctacaaa gaggctgcgg cacaggctca gcagcaacag
     1261 caaggaaaca atacgggcag cacgcagtcc acggacaatt atcaggaaac cccatatact
     1321 gatgttgata acaacgaata aaaaatatag caggggctct gcctctgcta tatttaaagc
     1381 tgtaagttga agggcgcaaa gagcccttga atcaacgctt taaatctaat ataattattt
     1441 cattgcgcgg caaaaactgc cgctctccaa aacaaaaggg ataaggttaa cttatggctg
     1501 aaaaacgaga ttattacgag gttttgggtg ttgacagaaa tgccaccgaa gacgaaataa
     1561 aaaaggcata cagaaggctt gccaagcagt atcatcccga cttaaacccc ggcgacaagg
     1621 aagccgaagc caagtttaaa gaggcaaacg aggcatacga ggtcctctcc gacagcgaaa
     1681 agagggccaa gtatgaccgc tttggccatg cgggcgttga cccgaattac ggcgcaggtc
     1741 agggc
//
LOCUS       DAONVQ010000190         1740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_45421_length_1740_cov_4.891395, whole genome shotgun sequence.
ACCESSION   DAONVQ010000190 DAONVQ010000000
VERSION     DAONVQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45421_length_1740_cov_4.891395"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLD98_08885"
     CDS             <1..188
                     /locus_tag="PLD98_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06397.1"
                     /translation="SFTLEYMQDGDFDVPTEIFVPGKISVLELPEGAAAETEENDNCT
                     ILKIKSQVGKNRIKVSW"
     gene            291..659
                     /locus_tag="PLD98_08890"
     CDS             291..659
                     /locus_tag="PLD98_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQA06398.1"
                     /translation="MEKINDNLTPACDCSVIHEDVVREVGKSMPDDEVMFNLAEFFKI
                     FGDSTRVRIICALFRSEMCVCDIAALLGMTKSAISHQLRILKQSKLVKNRRNGKVVYY
                     SLDDEHIAKIIDQGLAHILE"
     gene            726..>1740
                     /locus_tag="PLD98_08895"
     CDS             726..>1740
                     /locus_tag="PLD98_08895"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQA06399.1"
                     /translation="MKLVQEKKADYEVDIEKLIRSSAENEYSFDFSVKNLDCPDCASK
                     TEQAVSRQKGVKSANLNSLQMKLNIEIEPNEDRAEVLKSAISAAKNAHPGIELAASPK
                     QEPLHKSSNDALDDDEEKEKRIMIIRLIVSSVLFGIGFLFGDGSVPSLIINLIAYLII
                     GGEVLLKAFRNILKGKVFDENFLMSVATIGAFFIKSYHEAAAVMLFYQVGEFFQDLSV
                     SKSRKSIKALMELKPYFANLLVGEEEVKVDPAKVAVGSHIIVKPGERIPIDGIVLQGS
                     GLVDTSALTGESMPREANEGDEVLSGTINLNSVLVLKTIRSLETSAVSRILELVEKAG
                     EKKA"
BASE COUNT          525 a          366 c          415 g          434 t
ORIGIN      
        1 aatcctttac cctcgagtac atgcaggatg gcgattttga tgttcccacc gagatatttg
       61 tcccgggaaa aataagcgtt ttagagctcc ccgaaggcgc cgctgccgaa acagaggaga
      121 acgacaactg caccatcctc aaaattaaat ctcaggtagg caaaaaccgc ataaaagtaa
      181 gctggtaaaa aaaatctccg catgatgcgg ggatttttta ttttgtgttg acatttgtat
      241 ggtacgagca tataatacag ttgaacggtt attcaactga gaggtgcgaa atggaaaaaa
      301 taaatgataa tttaacccct gcctgcgact gctctgtaat tcacgaagat gtggtaagag
      361 aagtaggcaa atcaatgccc gacgatgagg ttatgtttaa ccttgcggag ttttttaaaa
      421 ttttcggcga ctccacaaga gtgcgcataa tctgcgctct tttccgttcg gaaatgtgcg
      481 tctgcgacat tgccgccctt ttgggcatga caaagtcggc aatttcccat cagctcagaa
      541 ttctcaagca gtcaaagctc gtaaaaaacc gcaggaacgg caaggtggtt tactactccc
      601 ttgacgacga gcacatcgcg aagattatag accagggtct cgcgcacatt ttagaataac
      661 cttttatttt gccatatggt tgaagatttg ttcacctgtt caatcttgat gaaaggataa
      721 agcttatgaa attggttcag gaaaaaaagg cggattacga agtggatata gaaaagctga
      781 ttagaagctc tgccgaaaat gaatacagct tcgatttttc tgtaaaaaac cttgactgtc
      841 ccgactgcgc aagtaaaaca gagcaggcgg tgtcgaggca gaaaggtgta aaatctgcga
      901 atcttaattc cctgcaaatg aaactgaata ttgagataga gccaaacgag gacagggcag
      961 aggtcttaaa gtcagccatt tccgccgcta aaaacgccca cccgggcata gagctggccg
     1021 caagtccaaa gcaggagcct ttacataaaa gctcaaacga cgctttggac gatgacgaag
     1081 agaaggaaaa gaggataatg attataaggc ttatagtcag ctctgtactg ttcggcatag
     1141 gtttcctttt tggcgacggc agcgtaccat cgcttattat aaatctcata gcatatctca
     1201 ttatcggcgg agaagtgctg ctgaaagcct tcagaaatat tctaaagggc aaggttttcg
     1261 acgagaattt ccttatgtcc gtcgccacga ttggagcgtt ttttataaag agctaccacg
     1321 aggcggcagc cgttatgctc ttttatcagg ttggcgagtt cttccaggac ctctccgtaa
     1381 gcaaatccag aaagtcaatc aaagccctta tggagctaaa accctacttt gccaacctgc
     1441 ttgttgggga ggaagaagta aaagtcgacc ccgcaaaggt agctgtcggt tctcatataa
     1501 tcgtaaagcc cggtgagaga atccccatcg acggtattgt cttgcagggc agcggtcttg
     1561 ttgacacatc agctcttacg ggtgaatcca tgccgagaga ggcaaacgaa ggcgatgagg
     1621 ttctgtcagg cacaataaat ctgaattcgg ttcttgtcct taaaacaatc agaagccttg
     1681 agacttcagc ggtttcaaga atccttgaac ttgttgaaaa agcgggagag aaaaaggcaa
//
LOCUS       DAONVQ010000191         1734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_45616_length_1734_cov_4.920191, whole genome shotgun sequence.
ACCESSION   DAONVQ010000191 DAONVQ010000000
VERSION     DAONVQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45616_length_1734_cov_4.920191"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLD98_08900"
     CDS             complement(<1..222)
                     /locus_tag="PLD98_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06400.1"
                     /translation="MSRLSLHSLSRREKLLVSIICLLSLLTFSFFLDYEKSKDKLEAV
                     YGSVTTKAATENLYDLQSELFGGETKTEPI"
     gene            complement(244..999)
                     /locus_tag="PLD98_08905"
     CDS             complement(244..999)
                     /locus_tag="PLD98_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06401.1"
                     /translation="MNFVKKLTASVAVISCLFAISACNRRSNPTIDVPDYSDPETTET
                     ETVSAPNFSSEWRYKYSLTYEYTKGKTVSVLSEYRDDGFFCAVDNESKVMTYYKQGDE
                     GIDRYILNPSTKKGTHAVLKGRSLEKLVSGFMQLSLSDPSFISKENVIYSSEDVIAGR
                     PAKKYIQMEYQDSVLTRIAYIWIDTEFGFASRCEVYDGTGGLAFGWKLTEFSREAPDA
                     ENLIPDISKYKLTELGVIDNGSESSAEETSAAD"
     gene            1321..>1734
                     /locus_tag="PLD98_08910"
     CDS             1321..>1734
                     /locus_tag="PLD98_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06402.1"
                     /translation="MTKRLKKQLTATLALVLALTSFAFALNAVAGDRLYLLSDKPFVV
                     DEENKVIYLYQEKVDEETFRSFFENAYDELVLSGLVPATIVVGEGDEAEEIETKLIAT
                     GSTLTILNEVGPLTAMCDADEDETEEPTDEPSDEPS"
BASE COUNT          428 a          394 c          425 g          487 t
ORIGIN      
        1 tatcggctct gttttggttt cgcccccgaa aagctcgctt tggaggtcgt aaaggttctc
       61 cgtggctgct tttgtggtaa cgctgccgta aacggcttcc agtttgtcct ttgacttttc
      121 gtaatccaaa aaaaagctga atgtcaatag tgagagaaga cagattatcg ataccagcag
      181 cttttcccgc ctgctgagac tatggagact tagccgactc aagtcctcac cccatttatt
      241 tctttaatcc gccgcggatg tctcctccgc agaggattcg ctgccgttgt caataacgcc
      301 gagctctgtg agcttatact ttgaaatgtc ggggattagg ttttccgcgt cgggggcttc
      361 ccggctgaac tcggtcagct tccagccaaa tgcaagtccg ccggtgccgt cataaacctc
      421 gcagcgggag gcaaagccga attctgtgtc aatccaaata taggcgattc tcgtcaggac
      481 gctgtcctga tattccatct ggatatattt ctttgcgggt ctgcccgcta tcacatcttc
      541 ggagctgtaa atcacattct cctttgaaat aaacgaggga tcgctgagcg acagctgcat
      601 aaagccggac accagctttt caaggctcct gcccttcagc acagcatggg tgcccttttt
      661 ggtggagggg ttgagaatgt acctgtctat gccctcgtcg ccctgtttgt agtaggtcat
      721 aaccttgctt tcgttgtcaa cggcgcagaa gaatccgtca tccctgtatt ccgaaagcac
      781 ggaaacggtt ttgccctttg tgtattcgta tgtaaggctg tatttatagc gccattcgct
      841 tgagaaattg ggggcgctca cggtttcggt ctcggtggtc tcggggtcgg agtaatcggg
      901 aacatctatg gtgggatttg aacgcctgtt gcaggcggag attgcgaata aacaggaaat
      961 tacagcgacg ctcgcggtca gctttttgac aaaattcata attcctccgt gttttaggca
     1021 aaagccgaaa tggcttagac ttattatttt ttctctaacg gttattatga aataccctga
     1081 gccttttgtc aacataaacg cccttttttc gccatgttat ggggttcccg aaaggataat
     1141 ccgcgggcgg attatcagtt ttttaattct cccagcggag tgcaaacaaa cggaaattta
     1201 ggatattggt acaataattg ggactgtccg ccgccaaaaa ggttgactta tttccaaaaa
     1261 gaatctataa tttacttgtg aaaaattttg gaaaatatat acaaaaagga ggaacaggag
     1321 ttgactaaaa ggcttaagaa acaattgaca gccaccctcg cattggtttt agcgcttaca
     1381 tctttcgcat ttgctttgaa tgctgttgcc ggtgacaggc tgtatctgct ttctgacaag
     1441 ccctttgtgg tagatgaaga aaacaaggta atttaccttt accaagaaaa agtggatgaa
     1501 gagacattca ggagtttctt tgagaatgcc tatgatgagc ttgtgctctc gggtctggtt
     1561 cctgccacga ttgtagtggg cgaaggcgat gaggcagaag aaattgagac caaactcatt
     1621 gccacaggtt cgacccttac aattcttaac gaagtagggc ctttaaccgc tatgtgcgat
     1681 gcagacgagg acgaaaccga ggagcccact gatgagccct ctgatgagcc ctct
//
LOCUS       DAONVQ010000192         1720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_46131_length_1720_cov_2.989790, whole genome shotgun sequence.
ACCESSION   DAONVQ010000192 DAONVQ010000000
VERSION     DAONVQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46131_length_1720_cov_2.989790"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..>1720)
                     /locus_tag="PLD98_08915"
     CDS             complement(65..>1720)
                     /locus_tag="PLD98_08915"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HQA06403.1"
                     /translation="LKKEVEGNKRLPKWCGELYLEFHRGVLTSQAINKWYNRKSEVLY
                     QNIENAAVLEKAVTKSGFEKYPKTGIDKGWEIILLNQFHDIIPGSSIKEVYEDSKKQY
                     EQITQDGEYMLSSSLSRIAKGVAAEGRVLVVFNTLGSVRNDIVETEAPAADDFTLMDM
                     EGNVVPYQRTHDGKIVFFAENVPAKGYKSYLVSPAPYSDSDTAAVFSGNKVTSRFFNI
                     EFDDDMNISRLEDRRTGRLVAPEGKKLNQLIAYEDRPFNHDAWNVQCYFEEKAEEIND
                     VLNARVIENGPVRAVLMIVRRFKSSLITQYIKIYKNIDRIDIQNVVDWKEAYYIVKAL
                     FPVDVNSVKATFDIQFGNIERTTHNNTLWDFAQYEVAAQKWADLSDNSFGLSVLNDCK
                     YGYDVKHGVVRLSLLRSQNFPCKGQDQHVHYFTYSIYPHEGAVQHSDVVTHAYNLNYP
                     LLAYTGDGLGSLPAEFSLLSVDQDNIIVETVKKAEDSDEVVIRMFETWNRRTRCAVNF
                     GVNVEKAVECDMMEENEKPLTITSNVLDLEFRPFEIKTIKIKI"
BASE COUNT          368 a          416 c          445 g          491 t
ORIGIN      
        1 cctcaaagcg tttgagcttt ttaagcattc tatcaatttg catctggttg agcataccgc
       61 acctctaaat ctttatttta attgtcttaa tctcaaaggg tctgaactca aggtcaagaa
      121 cattggaagt gatggtgagg ggcttttcgt tctcctccat catgtcgcac tcgacggctt
      181 tttccacatt cacaccgaaa ttgacggcgc atcttgtgcg tctgttccat gtttcaaaca
      241 ttcttattac gacctcgtcg ctgtcctcag cctttttgac cgtttcaaca atgatgttat
      301 cttggtcaac gctcagaagc gagaactccg cgggcaggct gccgagaccg tcgccggtat
      361 aagccaaaag cggatagttg aggttataag cgtgggtgac gacatcggag tgctgtaccg
      421 ctccctcatg gggatagatt gaatatgtga agtagtggac atgctggtcc tgacccttgc
      481 agggaaagtt ctggctgcgc agtaaggata gtctgacaac tccgtgtttt acatcatagc
      541 cgtacttgca gtcgttgaga acggacagac cgaagctgtt gtcgctgagg tcagcccact
      601 tctgagcggc aacctcgtac tgggcaaagt cccaaagggt gttgttgtgg gtggttctct
      661 cgatgttgcc gaactggatg tcaaaggttg cctttacgga gttgacatca acggggaaca
      721 gagcctttac gatgtagtag gcctccttcc agtcaaccac attctgaatg tctattctgt
      781 caatgttctt gtaaatctta atgtactgag tgatgagtga gctcttgaac ctgcggacta
      841 tcatcagcac tgccctgacg gggccgtttt cgattactct cgcgttgagg acatcgttga
      901 tttcctcagc cttctcctcg aaatagcact ggacattcca tgcgtcgtgg ttgaagggac
      961 ggtcctcata ggcgatgagc tgattgagct ttttgccctc gggggcgaca agcctgcctg
     1021 ttcttctgtc ctcgagcctt gaaatgttca tatcatcgtc aaactcgatg ttgaagaacc
     1081 tggaggttac cttgttgccg ctgaatacag ccgcggtgtc ggaatcggaa tagggcgcgg
     1141 ggcttacaag gtaggacttg tagccttttg cggggacatt ctcggcaaag aacacaatct
     1201 tgccgtcgtg ggttctctga tagggaacca cattgccctc catgtccata agggtaaagt
     1261 cgtcagcagc gggcgcttcg gtctcgacta tgtcgttcct tacgctgccg agtgtattga
     1321 acacgacgag aactctgccc tccgcggcga ctcccttggc tatgcggcta agggaactgg
     1381 agagcatgta ttcgccgtcc tgcgtaatct gctcatactg ctttttggag tcttcataga
     1441 cttccttaat tgaggagccg gggataatgt cgtggaactg gttgagaagg ataatctccc
     1501 agcccttgtc aatgccggtt ttggggtact tttcaaatcc gctctttgtg acagcctttt
     1561 caagaactgc ggcgttttcg atgttctggt aaagtacctc ggactttctg ttgtaccatt
     1621 tgttgattgc ctgagaggta agaacgcctc tgtggaactc aaggtacagc tcgccgcacc
     1681 atttgggcag gcgcttgttg ccctcaactt cctttttaag
//
LOCUS       DAONVQ010000193         1625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_49872_length_1625_cov_3.761783, whole genome shotgun sequence.
ACCESSION   DAONVQ010000193 DAONVQ010000000
VERSION     DAONVQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49872_length_1625_cov_3.761783"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /gene="rsgA"
                     /locus_tag="PLD98_08920"
     CDS             complement(<1..634)
                     /gene="rsgA"
                     /locus_tag="PLD98_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQA06404.1"
                     /translation="MSKALLNATIIKGVGGLYTVLADDGSTLECKARGIFRKDNIVPL
                     VGDKVLVSLKDGSNVIEEILERRNFLLRPALSNIDRLVIVSSVIEPRPNLLLIDRLAC
                     LAIYNNIEPVIVFTKTDLADACNLKRIYTSAGFKVFTIGFGKEEEVGELIETLSSGIS
                     AFAGNTGVGKSTLINLIEPSLNLSTGEISKKLGRGRHTTRSLELFRFKDGF"
     gene            complement(646..>1625)
                     /locus_tag="PLD98_08925"
     CDS             complement(646..>1625)
                     /locus_tag="PLD98_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HQA06405.1"
                     /translation="LAQCTPRQGIEASNNSQSSSESQVRAGPSAHPAPPPEAGQYAAY
                     GEVIFVYYSTKVNTNVIYAPNTVGNTLSVAKRQLEAVKLKVGKITYAPSSVALKDFVL
                     SQNPPASSPMQPNMVVDLVVGSGVPESSNVDITVTLPKLASGSTGNVQVFIGNTLANS
                     YKSVLMDGGSYSINLSGKGSNNTFKIYVDDLLLCEGKVDFTKEPAEISNRKDYKILLM
                     QNVENIQYEKAVEKLVSAGFAKANITAEYAFNEKVPIGYVYAQSPKDIKGYPADQKIT
                     LYVSRGPEPTTAPPVTTTQPPVTTEPPAPPQTTESATQPANATTVKTTE"
BASE COUNT          346 a          374 c          451 g          454 t
ORIGIN      
        1 aaaaaccgtc tttgaatctg aacagctcaa ggctgcgcgt ggtgtgtctg cctctgccca
       61 gctttttgct tatctcgcct gtggagagat taagcgacgg ctcaatgagg tttattagcg
      121 tcgatttgcc tacgcccgta ttgcccgcaa aagcggaaat tccgcttgaa agggtttcaa
      181 taagctcccc tacttcctcc tctttgccaa agccgatggt gaatacttta aaacccgctg
      241 aggtgtaaat tcttttgaga ttgcaggcgt cggcaaggtc ggtttttgta aagacgatta
      301 caggctcaat attattataa atcgcaaggc aggcgagcct gtcaatcaag agaaggttgg
      361 gtctgggctc tatgaccgat gaaacgatta cgagcctgtc gatatttgaa agggcgggac
      421 gcagaaggaa attccttcgc tccaagattt cttctattac atttgagccg tctttgagac
      481 ttacaagcac tttgtctccg accaaaggaa caatattgtc cttgcggaaa atgccccttg
      541 ccttgcactc taaggtgctg ccgtcatcgg cgaggacagt gtataatccc cctacgcctt
      601 tgataatggt ggcattcagt agagctttgc tcataaatcc cccgcttatt cagtagtttt
      661 taccgttgtg gcgtttgctg gctgagtagc agattcggtt gtctgaggcg gagctggcgg
      721 ctccgttgta acaggcggct gtgttgtggt aaccggcgga gctgttgtcg gctcgggtcc
      781 gcggcttaca taaagggtaa tcttctggtc tgccgggtag cccttaatgt ctttaggtga
      841 ctgcgcatag acatagccga ttggaacttt ttcgttaaag gcgtactcgg cggtgatgtt
      901 tgccttcgcg aagcctgcgg aaacgagttt ctccacagcc ttttcgtact gaatattttc
      961 aacattctgc atcagcagta ttttgtagtc ttttctgttt gaaatctccg cgggctcttt
     1021 tgtaaagtca accttgcctt cgcaaaggag caggtcatcc acatagattt tgaatgtgtt
     1081 gttgctgccc ttgcctgaga ggttgatgct gtagctgccg ccgtccataa ggacgctctt
     1141 gtagctgtta gcgagtgtgt tgcctataaa gacctgaaca ttgcccgtag agcccgacgc
     1201 gagcttgggt aaagttacgg tgatgtccac attggagctt tcgggaacgc cgctgcccac
     1261 aacaaggtca acgaccatgt tgggctgcat gggtgagctg gcgggcgggt tctggcttaa
     1321 aacaaagtct ttaagagcaa cggagctggg ggcgtaggtt attttgccca ctttgagctt
     1381 aacagcctcg agctgtctct tagcgacaga gagggtgttg cccacggtat tgggggcata
     1441 gatgacattg gtgttgacct ttgtggagta atagacgaat ataacctctc cgtatgcggc
     1501 gtactgacct gcctcgggag gtggggctgg gtgcgcggat gggccggccc gaacctgtga
     1561 ttcggatgag ctttgtgaat tgttgctagc ctcgatcccc tggcgcggcg tacattgagc
     1621 aagtg
//
LOCUS       DAONVQ010000194         1580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_51900_length_1580_cov_3.773770, whole genome shotgun sequence.
ACCESSION   DAONVQ010000194 DAONVQ010000000
VERSION     DAONVQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51900_length_1580_cov_3.773770"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLD98_08930"
     CDS             complement(<1..190)
                     /locus_tag="PLD98_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HQA06406.1"
                     /translation="MGKDSTLLYYDRPADKWTEALPLGNGKLGAMVFGRVKDERISLN
                     LDELWSGYPNDAYNEKAYS"
     gene            complement(204..344)
                     /locus_tag="PLD98_08935"
     CDS             complement(204..344)
                     /locus_tag="PLD98_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06407.1"
                     /translation="MNLAKELNLKICGGTDFHAGTKPHIAIGKGLGNLKIPYEVLEQI
                     KA"
     gene            complement(351..1037)
                     /locus_tag="PLD98_08940"
     CDS             complement(351..1037)
                     /locus_tag="PLD98_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQA06408.1"
                     /translation="MSRYIDLHTHSNKSDGTMSPAELVRHAKENGLAAIALTDHDTID
                     GLDEALSEGERMGLEVVPGIELSVMSDTETHILGLFIDPKSTALDRAMKRIIDSRNER
                     NRETSRLLKKLGFDVSVEEAQELATGGIVGRIHFAKIMVQKGYCKTVKEAFDEYLATG
                     KPAFCNRQGITAKEAIEIIRDSGGYSFAAHLHLMRRDDDNLKAYIKELKDYGLDGIEA
                     YYTDYTDEMH"
     gene            complement(1057..>1580)
                     /locus_tag="PLD98_08945"
     CDS             complement(1057..>1580)
                     /locus_tag="PLD98_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06409.1"
                     /translation="INKLFNGRNMNTNIRLILKAKEILRNTTPLPFDCGTLCENLCCK
                     GSEDVGMLLFPDEEHLYINSGNFEIIDGKIIVCKGHCDRDERPLSCMLYPLFPVISEE
                     SGKTRIRLIPDIRGSIICPLTAEENLVTRDFERAVRRAVRCLIVSPEHRRFIDNISEE
                     LSEIYTLRNLLIK"
BASE COUNT          386 a          387 c          310 g          497 t
ORIGIN      
        1 cggaataggc cttctcattg taagcgtcat tcggatagcc cgaccacagc tcgtcaaggt
       61 ttaaagagat tctctcatcc tttactctcc cgaaaaccat agcccccagt tttccgtttc
      121 ccaaaggcag agcctccgtc cacttatcgg caggtctgtc gtaatataaa agcgtgctgt
      181 ccttgcccat aaaaacacct catttatgct tttatctgct ctaaaacctc atatggaatc
      241 ttcaaattgc caagcccttt gccaatggca atatggggct ttgttcctgc gtgaaaatcc
      301 gtgccgccgc agattttgag atttagctcc tttgctagat tcatatattt ctaatgcatt
      361 tcatcggtgt aatcggtata gtatgcttct attccgtcaa gaccgtagtc tttaagctcc
      421 ttgatatacg cctttagatt gtcgtcatct cttctcataa ggtgaagatg cgcggcgaaa
      481 gaatagccgc ccgaatccct gataatctca attgcctctt tggcggtaat gccttgacgg
      541 ttgcagaaag cgggcttgcc tgtggcaagg tattcgtcaa aagcctcttt tacggttttg
      601 cagtaaccct tttgaaccat aatcttagca aagtgaatcc tgcccacaat gccgcctgtt
      661 gctaattcct gcgcttcctc aacggaaaca tcaaagccta gcttttttaa gagtctgctt
      721 gtttcgcggt ttcgctcgtt tctgctgtct attatccttt tcatggctct atcaagagcg
      781 gtgcttttgg ggtctataaa caagcctaaa atatgagttt cggtatcaga cattacggac
      841 agctcaatac cgggtacgac ctcaagaccc attctttcgc cttctgaaag agcttcgtca
      901 agaccgtcaa tcgtgtcatg gtcggttaga gcaatcgccg caagaccgtt ttccttcgca
      961 tggcgcacaa gctccgcggg gctcatagtg ccgtcggatt tatttgaatg tgtgtgcaaa
     1021 tcaatatacc ttgacataaa acttcctttt tttatgctat ttgataagca gatttcttag
     1081 agtatatatt tcgctcaact cttcgctgat attatcaata aaccttctat gttctgggct
     1141 tacgataagg cagcgcactg cccttctgac cgccctttca aaatcccttg ttaccaggtt
     1201 ttcctcagct gtcagcggac agattatgct ccctcgtata tcaggtataa gcctgattct
     1261 tgttttgccg ctttcttcgc tgataacagg gaaaagagga tacagcatac atgaaagggg
     1321 tctttcatct ctatcgcagt gccctttaca aactataatt ttgccgtcaa ttatctcaaa
     1381 attcccgctg tttatgtata aatgctcttc atcggggaaa aggagcatac ccacatcctc
     1441 agagcccttg cagcaaaggt tttcgcacag cgtgccgcag tcaaacggga gcggagtggt
     1501 attccttaat atttctttag ctttcaaaat aagtctgata ttagtattca tatttcttcc
     1561 gttaaacagt ttattaatat
//
LOCUS       DAONVQ010000195         1539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_53806_length_1539_cov_3.909704, whole genome shotgun sequence.
ACCESSION   DAONVQ010000195 DAONVQ010000000
VERSION     DAONVQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53806_length_1539_cov_3.909704"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..756
                     /gene="sfsA"
                     /locus_tag="PLD98_08950"
     CDS             76..756
                     /gene="sfsA"
                     /locus_tag="PLD98_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HQA06410.1"
                     /translation="MKYERIKRAKFIERKNRFIAEILIDGKLELCHVKTTGRCKELFI
                     KGADIFVQEVSVPGRKTKYDLISVYQNDMLINTDSQVANTLCLELIKHGGLFKDVKSI
                     RQEYGKLNSRFDFYVEYEDKKAFIEVKGVTLEENGVALFPDAPTSRGLKHINDLIELK
                     AMGYEAYIIFMVQMENALVFRPNIKQDKAFADGLKRAKIEGVNIKAFRCFVGEDSIYP
                     LSEIEVQI"
     gene            753..1262
                     /gene="thpR"
                     /locus_tag="PLD98_08955"
     CDS             753..1262
                     /gene="thpR"
                     /locus_tag="PLD98_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HQA06411.1"
                     /translation="MRLFIAINFDSETKSKIIDVQNKLREIAAGRFTRPENLHLTIFF
                     LGEVEDYRAILPCMKKNFTEEIELCFSGVGSFRSNLYWIGIKENPALNRLYRNLCADL
                     DKYGFKTDAKEIFKPHVTLAREVSVNKDKVNISFDEFKMSANRLSLMKSERINGKLTY
                     TEIYGVSVK"
     gene            complement(1340..1455)
                     /gene="rrf"
                     /locus_tag="PLD98_08960"
     rRNA            complement(1340..1455)
                     /gene="rrf"
                     /locus_tag="PLD98_08960"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1459..1534)
                     /locus_tag="PLD98_08965"
     tRNA            complement(1459..1534)
                     /locus_tag="PLD98_08965"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1499..1501),aa:Asn,seq:gtt)
BASE COUNT          500 a          286 c          366 g          387 t
ORIGIN      
        1 aataagctgg caaagtactt aaagaataaa taaaagaacg gggagcgttt ttgcgctccc
       61 ctaaataatg ttaatatgaa atacgaaaga ataaaaaggg caaaattcat agagagaaaa
      121 aacagattca ttgcggaaat tctgatagac ggaaagcttg agctttgcca tgtaaagacc
      181 acagggcgct gcaaggagct gtttattaag ggtgcggata tttttgtgca ggaggtgtcc
      241 gttcccggca gaaagacaaa atacgacctt atatcagtat atcagaatga tatgctcata
      301 aataccgaca gtcaggttgc gaacactcta tgccttgagc taataaagca tggcgggctg
      361 tttaaggatg taaagagcat aaggcaggaa tacggaaaac taaactcccg atttgatttt
      421 tatgtggagt atgaggacaa gaaagcattt attgaggtta aaggcgttac tcttgaagaa
      481 aacggggttg ctctctttcc cgacgctccc acatccagag ggctcaagca cataaatgac
      541 ctaattgagc ttaaagcaat gggatatgag gcttatatca tttttatggt tcaaatggaa
      601 aacgcccttg tgttcagacc gaatattaag caggacaagg catttgccga cggacttaaa
      661 agggcaaaga ttgagggcgt gaatataaaa gcattcagat gctttgtggg cgaggacagt
      721 atatatcctt taagtgaaat tgaggttcag atatgagatt attcattgcc attaactttg
      781 attccgaaac aaaaagcaaa atcattgatg ttcaaaataa actaagagaa atcgccgcgg
      841 gaaggttcac aaggcctgag aatctgcatt tgacgatttt ttttcttggc gaagtggagg
      901 attacagggc aattctccct tgcatgaaga agaactttac cgaagaaatt gagctttgtt
      961 tcagcggcgt tggcagcttt aggtcgaatc tctactggat tggaattaag gagaaccccg
     1021 cgcttaatag gctatacaga aacctatgcg cggatttgga caaatacggt tttaagacgg
     1081 acgccaaaga gattttcaag ccccatgtga cccttgcgag agaagtatcg gtaaacaaag
     1141 ataaagtaaa cattagcttt gatgagttta agatgagtgc gaacagattg tcgctgatga
     1201 aatcggaaag gattaacgga aagctgacat acaccgagat ttacggcgta agcgtaaaat
     1261 aaaaaagaaa gcaatatggg actcccccat attgctttta aaaagcaaag aactgctatc
     1321 attcgatagc agttctttgt gttggcatca cctattttcc caggcagctt cccgccaagt
     1381 atcttcggcg caactgagct taactaccgt gttcgggatg ggaacgggtg gatcctcagt
     1441 gcaatcgaca ccaactagtg gctccccctg ttggactcga accaacgacc ctgcggttaa
     1501 cagccgcatg ctctaccgac tgagctattg aggaatata
//
LOCUS       DAONVQ010000196         1535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_54004_length_1535_cov_4.435811, whole genome shotgun sequence.
ACCESSION   DAONVQ010000196 DAONVQ010000000
VERSION     DAONVQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54004_length_1535_cov_4.435811"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLD98_08970"
     CDS             <1..531
                     /locus_tag="PLD98_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HQA06412.1"
                     /translation="LSVEPVVTADENDYALRESDLPRIFEEYERLANIILERKLKGNC
                     FNFFHFMIDLEQGPCAIKRLRGCSCGNEYIAVTPNGDIYPCHQFVGMDEWKMGNVLDG
                     SLDYEKKKYFSKTHIYYKDECKNCWARFYCSGGCNANNYASRGSVLKPHRLSCELEKK
                     RLECAIMIKVALAAAE"
     gene            646..1266
                     /locus_tag="PLD98_08975"
     CDS             646..1266
                     /locus_tag="PLD98_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HQA06413.1"
                     /translation="MKFRTGKVIRVSANLIKVLKEYYRLVLLISLFIAGMLIGAFTFK
                     HGGGDVSSLLTDMVIKHSAERTSQAAFVTFINSLLVNCAFVGIAFTMGMCCIGTPFIG
                     LLPLIKGLGLGVVAGYFYNNDVAGVLYYLLIILPGAVISTVALIISCGESIRMSSEML
                     QIMSQKKSASPDGIKVYMSRYTIILALILISSVIDMFLTKAFSFLF"
     gene            complement(1292..>1535)
                     /locus_tag="PLD98_08980"
     CDS             complement(1292..>1535)
                     /locus_tag="PLD98_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQA06414.1"
                     /translation="NFSGLFIAVGSAAAGDLARKLGANVADNKIEVNNDMETNIPGLY
                     AAGDCTGGIMQVSTAVAEGAKAALNAIDYVRKKKKV"
BASE COUNT          396 a          351 c          375 g          413 t
ORIGIN      
        1 ctctctgtcg agccggttgt aaccgccgac gaaaacgact atgcgctgag agaaagcgat
       61 ttgcccagaa tctttgagga atacgagaga ctggcgaata taatccttga aagaaagctc
      121 aaaggcaatt gctttaattt cttccacttt atgattgacc ttgagcaggg tccctgcgca
      181 attaagaggc ttaggggctg cagctgcgga aacgagtaca tagcggtaac tcccaacggc
      241 gacatttacc cctgccatca gtttgtgggc atggatgaat ggaagatggg caatgtcctt
      301 gacggctctt tggattatga aaagaaaaag tatttctcaa agacccatat ttactacaag
      361 gacgagtgca agaactgctg ggcaaggttc tactgcagcg gtggctgcaa cgccaacaac
      421 tacgcgtccc gcggcagcgt tttaaagcct cacaggctct catgcgagct tgagaagaag
      481 cgtcttgaat gcgccataat gattaaggtt gccttagctg cggcagaata actcattaaa
      541 aagatttaac cgccgaaaaa aacggcggtt atattttttt atcctacggc atagttttat
      601 ttagggacaa cccttaaaag ccctaagcaa aacggaggaa gcgttatgaa attcaggaca
      661 ggcaaggtaa tcagggtttc ggcaaatctc attaaggtgc tgaaagaata ttacagactg
      721 gttttattaa tcagcctttt tatcgcgggc atgcttatcg gagcctttac atttaagcac
      781 gggggcggag atgtatcttc gctgctgacc gacatggtaa ttaagcacag cgcggagagg
      841 acatctcaag cggcgttcgt gacctttata aactctctgc ttgttaactg cgcttttgtg
      901 ggcattgcct ttacaatggg aatgtgctgc atcggcactc cctttatagg acttttgccg
      961 cttataaagg gcctcgggct cggcgtggtg gcaggctact tttacaataa tgatgtggcg
     1021 ggagtgctgt attatctcct tattattctc cccggcgcgg taatatcgac ggtcgcgctg
     1081 attataagct gcggggagag cataagaatg tcaagcgaga tgctgcagat tatgtctcag
     1141 aaaaagtccg cctcccccga cggcattaag gtttatatgt caagatacac gataatactg
     1201 gcgctgatac ttatttcttc cgtaattgac atgttcctta caaaggcgtt ttcgttcctg
     1261 ttctaagaag aaaaaagccg cttaaagcgg cttaaacttt tttctttttt ctaacatagt
     1321 caatagcgtt taaggctgcc ttcgcgcctt ccgcaaccgc tgtggaaacc tgcattattc
     1381 ctcccgtgca gtcccccgcc gcataaaggc cggggatatt tgtttccata tcgttgttga
     1441 cttcgatttt gttgtccgca acattcgcgc caagctttct tgccaggtcg ccagcggcgg
     1501 cgctgcccac agcgatgaac agtccgctga aatta
//
LOCUS       DAONVQ010000197         1531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_54184_length_1531_cov_6.725610, whole genome shotgun sequence.
ACCESSION   DAONVQ010000197 DAONVQ010000000
VERSION     DAONVQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54184_length_1531_cov_6.725610"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            445..978
                     /gene="raiA"
                     /locus_tag="PLD98_08985"
     CDS             445..978
                     /gene="raiA"
                     /locus_tag="PLD98_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQA06415.1"
                     /translation="MNITIIGRKCTPRASFKERAEKKLKKVERFFGEDVTAKITVTVE
                     KNVQTVEITLIKDGMIFRAEEKAEHMNEALDLCVDVLVRQIRKNKTKLAKRFKAAGLE
                     DLNGGDSAEVQEELDFNIVRTKTVPLKPQTVDEAILQMNMLGHQFYMFLNAETDKISV
                     VYVRHDGGYGMLEPELI"
BASE COUNT          431 a          376 c          352 g          372 t
ORIGIN      
        1 cgcatttttc agcggcaatc cgggggcaat ctgctccata ttttgctcgc ccacggctgg
       61 ggcagggaat acccgcctca ttttctcagc ggcaatcggg cgggggcttg tccgctcccc
      121 gactgcggac tacccccgca aagagcagcc gcgctcgcca acagcgcaag gaataagctc
      181 tcccccgctt cctgcccgct cccgtccgag caagcggatt cgggctttct gcctctcccc
      241 ggcgggcaaa actttgttaa aaaattcttt attaagaata agtaaacatt atctcatttc
      301 ggtttacaat ccgctcaact gatggtatac tttaatcaag acggtaataa aaaatatata
      361 acggaaggcg gtgttgggag aaaaactctc agggcagcct tccgtgtgtt tttaaccgtc
      421 attgccaaga agggggatgt atttatgaac attaccatta taggtcgaaa gtgcacgcct
      481 cgtgcatcct tcaaagaaag ggccgaaaaa aagctcaaga aagttgaacg gttcttcgga
      541 gaggatgtaa cagccaaaat cacggtcaca gttgaaaaga atgtccagac cgttgagatt
      601 accctcatca aggatggcat gattttcagg gctgaggaga aggctgaaca catgaacgaa
      661 gctcttgacc tgtgcgtgga tgttcttgtt cgtcagatac gaaagaacaa gacaaagctg
      721 gcaaagcgct tcaaagcggc aggtcttgag gatcttaacg gaggcgacag cgccgaggtg
      781 caggaggagt tagactttaa catcgtccgc acaaagaccg ttcccctcaa gcctcagacc
      841 gtggatgagg caatccttca gatgaatatg ctcggacatc agttctatat gttcctcaac
      901 gccgaaaccg acaagatttc ggtagtctat gtaaggcatg acggcggcta cggcatgctg
      961 gaacccgagc tgatatagag aattacggca gccccctgcc gaaacagcgc ccgaccgccc
     1021 gcgcggtcgg gctttttgcg gcgttttaac aaaagaaaca aaggcaaaag cttttattac
     1081 aattattaca ttggtttaca ttcatgttac tttcctttac tttccatttt tcagagccta
     1141 taatgcacat tggcggaaat attttacata atcagcggga ctaaaatgcg ctttcgcttt
     1201 tgcgccttat gcttatctat ttgcattata taaaaaaact cagatgaaac tgagcaaacc
     1261 gccgctgctc agacagaacc cgcaggcaaa gccgataaga cccgttcttt taaagccgca
     1321 aggaaattcg catgtcacct gcgatacgcc aaagagcttt tttctccgcc gaattttact
     1381 gtctcggcga cagccctcag caacctgcgt tttagacaat gtcacgggcg agtattcaga
     1441 gccttagaac atcgcccgtc agacatggct ttattactgc aataagaaca aaaagttacg
     1501 aaagtaaaat atttattaat aattattact a
//
LOCUS       DAONVQ010000198         1326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_66613_length_1326_cov_4.618411, whole genome shotgun sequence.
ACCESSION   DAONVQ010000198 DAONVQ010000000
VERSION     DAONVQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66613_length_1326_cov_4.618411"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PLD98_08990"
     CDS             complement(<1..846)
                     /locus_tag="PLD98_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQA06416.1"
                     /translation="MADKDTKAVQTDSSGEFSAEQINYVYENRRYVGRKETIAYVMFD
                     MAQSININKYSGRFVNNILQIDFSLQQLATFINGIWDIINDIFTGAIVDKTRTRWGKF
                     KPYLIGLGIPGTMGAIIYWLMPLFFPQTGPKDLNKFLMYLILAVVREGVDTFRGIAQT
                     GMLATITPHPVDRTRLITIANFASGFLGEKLPEQIMTLLLDLIGNGIIKSETKSITQI
                     YTSLFVSMGVITSIISGAFSLYFFFVTRERVLQSVKKPSVMQGIKSILNNKPILLLTL
                     SQTLSG"
     gene            complement(988..>1326)
                     /locus_tag="PLD98_08995"
     CDS             complement(988..>1326)
                     /locus_tag="PLD98_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQA06417.1"
                     /translation="GEVITPALTGSILGGITRLSCIELLREHGYKVTERLISIDEIAK
                     AYDEGRLNEAFGSGTAAVISPIGELRWGDKVMKLSDGQIGPISRFLYDTLTGIQRGDI
                     EDTRGWIQKI"
BASE COUNT          320 a          318 c          322 g          366 t
ORIGIN      
        1 tcccgaaagc gtctgagaaa gcgtcagcag cagtatcggc ttgttgttga gaatcgattt
       61 gatgccctgc attacgctgg gtttcttgac cgactggaga accctctctc tcgttacaaa
      121 gaagaagtac agcgagaagg cgccgctgat aatgcttgtg ataacgccca tgcttacaaa
      181 gaggcttgtg taaatctggg ttatcgactt agtttcgctc ttaatgattc cgttgccgat
      241 aaggtcaagc agcagagtca ttatctgctc gggcagcttt tcgccaagga agccggatgc
      301 gaagttggcg atggtaatca gcctcgttcg gtcaacaggg tgaggcgtga ttgtcgccag
      361 catgcctgtc tgggcgatgc ctctgaaggt gtcaacgcct tcacggacga ctgccagtat
      421 aaggtacatg aggaacttgt ttaagtcctt ggggccggtc tgagggaaga acaggggcat
      481 gagccagtaa attatcgcgc ccattgtgcc cgggattccg agaccgatta agtaaggctt
      541 aaacttaccc cagcgggtac gagttttgtc aacaatagcg ccggtaaata tatcgttgat
      601 gatatcccaa ataccgttta taaaagtagc cagctgctgc aggctgaagt caatctgaag
      661 aatattgttt acaaatcgtc cgctatactt gttgatgttg atgctctgcg ccatgtcgaa
      721 cataacatac gcgattgttt cttttctgcc gacataacgc ctattttcat agacatagtt
      781 gatctgctct gcggagaatt cgccggagct gtcggtctgc acagccttag tgtctttatc
      841 cgccacgccc atacctccgt ttaatttttt tgcaacagta atactatatc ataacctata
      901 aaaaagcgca aggttcaaaa tccctttttg ttcataatta tcaatgccac ttatttccag
      961 agatgcaagg cgttgacgga ccttgtgcta tatcttctga atccagcctc ttgtatcctc
     1021 aatatctccc cgctgaattc cggtgagagt atcgtaaagg aatctggaga tgggaccgat
     1081 ttggccgtct gagagcttca ttactttatc tccccatctc aactcgccga tgggagagat
     1141 aaccgcggct gtgccgcttc cgaatgcctc gttgagcctg ccctcgtcat aggctttcgc
     1201 aatctcgtca atggagatga gcctttcggt tactttgtag ccgtgctctc ttaaaagctc
     1261 tatgcatgaa agtcttgtaa tgccgccgag aatactgccc gtaagagcgg gggtaatgac
     1321 ctcgcc
//
LOCUS       DAONVQ010000199         1324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_66790_length_1324_cov_2.356186, whole genome shotgun sequence.
ACCESSION   DAONVQ010000199 DAONVQ010000000
VERSION     DAONVQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66790_length_1324_cov_2.356186"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLD98_09000"
     CDS             <1..464
                     /locus_tag="PLD98_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQA06418.1"
                     /translation="LNIPCISVSTLQAIAYPLMGTGCIAAAVMDARCNQVYSADFLCQ
                     NNMERLCVDSAVSLDELKSRLSSYNIDSSIVFIGDGAEVAHKYFNERYPEFKVGIVAG
                     TVRFQSASSVAIIGCEEFEKGKTIDASVLVPIYLRPSQAERELKRKTALQR"
     gene            508..954
                     /gene="rpiB"
                     /locus_tag="PLD98_09005"
     CDS             508..954
                     /gene="rpiB"
                     /locus_tag="PLD98_09005"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HQA06419.1"
                     /translation="MIALGADHGGFHLKEAAKEYLKEKGIEFTDFGIYEEKSVDYPDI
                     AKTVCEAIQSGKCRYGILFCGTGIGMSIAANKHKGIRAACCSDYFSAKYTRMHNDANV
                     LCLGGRVVGTGLAIELIDAFLNSEFEAGRHAKRLEKIAEIENSIGG"
     gene            956..>1324
                     /locus_tag="PLD98_09010"
     CDS             956..>1324
                     /locus_tag="PLD98_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HQA06420.1"
                     /translation="MEDFLAQAKQVQWFPGHMAKTLRQLKELMPKVDIVIEITDARIP
                     ESSRNPELPSLIGNKPHIILLNKSDLADPEINKKWIDYYKKQGINAVLTDCKSGGGLK
                     QFMTVLRSVSAPIKERNAQKG"
BASE COUNT          403 a          288 c          326 g          307 t
ORIGIN      
        1 cattaaacat cccctgcatt tccgtttcga ctttgcaggc aatcgcttat ccgcttatgg
       61 gaacagggtg catcgccgct gccgtaatgg acgcccgctg caatcaggtg tattccgcgg
      121 actttttatg tcagaataat atggagcgtc tttgtgtgga ttcggccgtg agccttgacg
      181 aacttaaaag caggctcagc tcatataaca tcgattcgtc catagtattt atcggcgacg
      241 gcgcggaggt agcgcataaa tacttcaatg agagatatcc cgaatttaaa gtcggtatag
      301 tagcgggcac tgtccggttt caaagtgcat cctcagtcgc aattatagga tgcgaggaat
      361 ttgaaaaggg caaaacgatt gacgcaagcg ttttggttcc catatattta agaccatctc
      421 aggcggaaag agaactgaaa aggaaaacag ctttacagcg ttaagtgaat tactttacag
      481 caaaataatt acggaaaggt gagaatcatg attgcattgg gcgcggacca cggcggattt
      541 catcttaaag aagcggcgaa agagtatttg aaagaaaaag gcattgagtt taccgacttc
      601 ggaatctatg aagaaaaaag cgtcgactat cccgacatag caaagaccgt ctgcgaggca
      661 attcaaagcg gcaagtgccg gtatggaatt ttgttctgcg gaacgggcat cggaatgtct
      721 atagccgcaa acaagcataa gggcattcgc gcggcatgct gctccgacta cttcagcgcc
      781 aagtacacaa gaatgcacaa cgacgcaaat gtcctctgcc tcggcggcag ggtagttggc
      841 acaggtcttg ctattgaact tattgacgca ttcttaaaca gcgagtttga agcgggcagg
      901 catgctaaga ggctcgaaaa aatagccgaa atagaaaatt caatcggagg ataaaatgga
      961 ggacttctta gctcaggcaa aacaggttca gtggttcccc ggtcacatgg caaaaacctt
     1021 aaggcagctc aaagagctta tgcctaaggt tgatattgta attgagatta ctgacgccag
     1081 aatacccgaa agcagcagga acccagagct tccttcatta atcggcaata aacctcatat
     1141 tattctcctt aacaaatcgg accttgccga ccccgaaatc aataaaaaat ggattgacta
     1201 ttataaaaag cagggtatca acgccgtcct taccgactgc aaaagcgggg gcggcttaaa
     1261 gcagtttatg acggttttga gaagcgtttc agctcccata aaggagcgca acgctcaaaa
     1321 gggc
//
LOCUS       DAONVQ010000200         1269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_70926_length_1269_cov_4.103789, whole genome shotgun sequence.
ACCESSION   DAONVQ010000200 DAONVQ010000000
VERSION     DAONVQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70926_length_1269_cov_4.103789"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLD98_09015"
     CDS             <1..280
                     /locus_tag="PLD98_09015"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HQA06421.1"
                     /translation="YLLPNEWNGPQAESFENFREYIYAHFEKFPEGVTETYIHPAVES
                     DELKGTSSVWKRRVWEYQLFADPKTRQHFEAIGIKLINYRDLKKMRGY"
     gene            702..1175
                     /gene="greA"
                     /locus_tag="PLD98_09020"
     CDS             702..1175
                     /gene="greA"
                     /locus_tag="PLD98_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQA06422.1"
                     /translation="MSETFILTKEWVEELKQELKMLRAKGRNEIAEKIKEAKSFGDLS
                     ENSEYDEAKSEQGKLEARIAEIEYTLQHAKVLDESTLGTDTVHIGSKVKVLDKNRNQE
                     ITYHIVGSPQVDPINRKISDDSPIGRALIGKSVGDVVEVDVPAGVLRFEILEISK"
BASE COUNT          426 a          248 c          285 g          310 t
ORIGIN      
        1 ctatctcctg cccaacgagt ggaacggccc gcaggcagag agctttgaga acttcagaga
       61 gtacatctat gcacactttg agaaattccc cgagggcgta accgagacat acatccaccc
      121 cgcagttgag tccgacgagc tcaagggcac atccagcgta tggaagagga gagtctggga
      181 gtatcagctt ttcgccgacc ccaagacaag gcagcacttc gaagctatcg gtataaaact
      241 cattaactac agagatttaa agaaaatgag aggctattaa tttaaattaa tcaaattaaa
      301 tccgaaagag ccgttttaaa aggcggctct ttcggaaaac gaatttggga aatattttaa
      361 agtataaaca aaaaattctc tggtaacata gcaataatga ataggacgag aataaactga
      421 atataagttg ttctgaaatt taatttcccc tgccgagctt ttacttcttg acagcaaaaa
      481 agtttttgtc tataatatgg tatcaataga caggcaggtt aaaaacctgc acattatgtt
      541 taaatagtta aaaagtcgtc cctgcctttt tcggtgcggg cggaattttt tacttaaaaa
      601 tgttggctca tagacctgtt ttcggtcaag ctaagttcaa atcaggaaaa taacaccatt
      661 aatatagaaa ataaaaatat caccttgagg ggatgaacga catgtctgaa acctttattt
      721 tgaccaaaga atgggttgaa gaacttaaac aagagcttaa aatgctcaga gcgaaaggac
      781 gcaacgagat tgcggagaaa ataaaagagg ccaaatcctt tggtgacctg tccgaaaaca
      841 gcgaatatga tgaggctaag tcagagcagg gcaagctgga ggcaagaatc gccgaaatcg
      901 agtatactct ccagcacgcc aaggttcttg acgagagcac ccttggcacc gacaccgttc
      961 atatcggctc aaaggtgaag gtacttgaca agaacagaaa tcaggaaatc acatatcata
     1021 ttgtcggctc acctcaggta gaccccataa accgcaagat ttccgatgat tcaccgatag
     1081 gcagagcctt aatcggcaaa tccgtcgggg atgttgtgga ggttgatgtt cccgcaggtg
     1141 tattgcgctt tgaaattctt gaaatttcaa aataattcgg cgggcgaatc catcgcccgt
     1201 ttttttataa atctcagatg tttaaagcag gagagggtat aatggcagaa gaaaagaatt
     1261 tgaacaggg
//
LOCUS       DAONVQ010000201         1201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_76765_length_1201_cov_5.648342, whole genome shotgun sequence.
ACCESSION   DAONVQ010000201 DAONVQ010000000
VERSION     DAONVQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76765_length_1201_cov_5.648342"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLD98_09025"
     CDS             complement(<1..413)
                     /locus_tag="PLD98_09025"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HQA06423.1"
                     /translation="MNFFDLHCDTITELHKKGESLKNNSLHISLEKADCFDAYKQVFA
                     IFIPDTLRGKTAADYFDEVCDFYYEQLSENKDSISPYIDFENQSKYKAILAVEGAAAA
                     AGTIEGLHHLYNRGVKLITLTWNADNEVASGCFSET"
     gene            complement(423..812)
                     /locus_tag="PLD98_09030"
     CDS             complement(423..812)
                     /locus_tag="PLD98_09030"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQA06424.1"
                     /translation="MDDRRLKGVLGEQIAARFLREKGYEIIAANFKSKCGEIDIIAKK
                     AELIVFAEVKTRQKDTLIAPYLSVNQEKRRKIILTAQVFLKLNPRYSKCSYRFDIIEI
                     FLDGQRFLNLNHLENAYTPQKYKKLDY"
     gene            complement(805..>1201)
                     /locus_tag="PLD98_09035"
     CDS             complement(805..>1201)
                     /locus_tag="PLD98_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQA06425.1"
                     /translation="LDYSIAIVSEKEIDEINILNAAMLAMRKAVAGLKIKPDFVLIDG
                     NRLPETGYPERYIVKGDSLSMSIAAASILAKVSRDRYMLSLDEQYPEYCFNKHKGYGT
                     ALHYQMLEKYGISPVHRRSFLKNLAKHNG"
BASE COUNT          301 a          271 c          256 g          373 t
ORIGIN      
        1 gtctccgaaa aacagccgct cgccacctcg ttgtcggcgt tccatgtaag ggttatcagc
       61 tttacacccc tgttatacag atgatgcagc ccttctatgg tgccagccgc ggctgcggct
      121 ccttcaaccg ccaaaatcgc tttgtacttg gattggtttt caaaatctat gtatggagat
      181 atagaatcct tgttttccga gagctgctca taataaaaat cgcagacctc atcaaaatag
      241 tctgccgcag tcttgcccct taaagtgtcg ggaatgaata tggcaaagac ctgcttgtaa
      301 gcatcaaagc aatctgcctt ctcaagggat atgtgaaggc tgttattttt taaggactct
      361 ccctttttgt gcagctcggt tatcgtatcg cagtgaaggt caaaaaaatt caaattacca
      421 cctcagtaat ccagcttttt atatttttga ggagtgtagg cgttttccaa atgatttaag
      481 ttcaaaaacc tttgtccgtc taggaagatt tctataatgt caaacctgta gctgcacttt
      541 gagtatcggg ggttcagctt taaaaagact tgagcggtca gaattatctt tcttcgcttt
      601 tcttggttaa cagacaaata gggagcaatc aaggtgtctt tttgccgagt tttaacctcc
      661 gcaaagacaa taagctcagc cttttttgcg atgatgtcaa tttccccgca tttggattta
      721 aagtttgcag cgattatctc atagcccttt tcccttaaaa accttgccgc tatctgctct
      781 cccaaaacgc cctttaatct tctgtcatcc attgtgctta gcgagattct ttagaaatga
      841 cctgcggtga acgggagaaa tgccgtattt ttcgagcatc tgatagtgca gagcggtgcc
      901 gtatccctta tgcttgttaa aacagtattc gggatattgc tcatccaatg acagcatata
      961 gcggtctcgg ctgacttttg ccagaatgga cgcggcggct atggacattg aaagtgaatc
     1021 gccttttact atgtatcttt cgggataacc cgtttcgggg aggcggttgc cgtcaatcaa
     1081 aacaaaatcg ggtttaatct ttagcccggc gacagccttt ctcattgcaa gcattgcggc
     1141 attcaggatg tttatttcat caatttcctt ttcgctgact atcgcgatcg aatagtcaag
     1201 g
//
LOCUS       DAONVQ010000202         1187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_78098_length_1187_cov_3.715548, whole genome shotgun sequence.
ACCESSION   DAONVQ010000202 DAONVQ010000000
VERSION     DAONVQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78098_length_1187_cov_3.715548"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..>1187)
                     /locus_tag="PLD98_09040"
     CDS             complement(82..>1187)
                     /locus_tag="PLD98_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQA06426.1"
                     /translation="NTVPHYLLGGMTLKYLTNTEGVFVYLPILVSLVVSLFALAVRLP
                     VANLLLRVGLGGLRKKKPERYKTVKAAAKRPLSLFVSAVLIYAFYSLGNTVTISILPE
                     DELPKVLVFLYGQTIYRILSTIVLISAFWLLYNVVGISMAALLSVSESKGRKIDANAS
                     SYIASGLKVLVAVFGAYFVLTKWGVDVSGLIAGLGIGGLAMALAAKDTASNLFSSISI
                     MLDKPFEIGDTIIIGGIKGKVVSLGLRSSRLEAPDGSIITYPNSSLASAIITDTSKKQ
                     SSKIEMSLSVAFSTSTEKLSEFMDEIRRIIREDEDTTDTEPIVYFNSFTASAMQLSIV
                     YEVYPEFEREIRARQNINTKVIEAAEKMEIKLV"
BASE COUNT          294 a          273 c          304 g          316 t
ORIGIN      
        1 aggaggacat gagcagcaca tacggctcat aataatcaac aaataaaagg gcagaaatct
       61 ctgcccttta tttttttgcg tttatacaag ttttatttcc attttttcgg cggcttcaat
      121 tactttggta ttgatattct gccgagctct tatctcccgc tcgaattcgg ggtagacctc
      181 gtacacgatt gaaagctgca tagcggaggc ggtgaatgag ttaaagtaga cgatgggctc
      241 ggtgtctgtt gtgtcctcgt cttctctgat aatccgcctt atctcgtcca taaactcgct
      301 cagcttttcg gtactggtgc tgaaagcgac cgagaggctc atctctattt tggagctttg
      361 ctttttggat gtgtcggtga taattgcgga ggcaaggctg gagttggggt atgttataat
      421 cgagccgtcg ggggcttcaa gcctgctgct gcgaagtccg agacttacga ccttgccctt
      481 aatgccgccg attataattg tgtcgccgat ttcaaagggc ttgtccagca ttattgagat
      541 gctgctgaaa aggtttgagg cggtatcctt tgcggcgagc gccatggcaa gaccgccgat
      601 gccgagaccc gctataagtc ccgaaacatc cactccccac tttgtcagca cgaaatacgc
      661 tccgaaaacc gctacaagca ctttaagtcc gcttgcgata tagctggagg cgttggcgtc
      721 tatctttctg ccctttgact cgctgacgga aagcagagcc gccatgctga tgcccaccac
      781 attgtaaagg agccagaagg cgctgatgag aactatggtt gagaggattc tgtaaattgt
      841 ctgaccgtat aggaaaacta aaaccttcgg cagctcgtcc tcgggcaaaa tactgattgt
      901 aacagtattt ccgagggagt agaaggcgta aatgagcact gcggagacaa aaaggctaag
      961 gggtcttttc gccgcggctt tgacggtttt gtagcgttcg ggtttctttt ttctgagccc
     1021 tccgagaccc acccttaaaa gcaggtttgc aacgggcagc cttacagcaa gggcgaagag
     1081 ggagacaacc aaggatacca agataggcag atatacaaaa accccttcgg tgtttgtaag
     1141 atacttcaag gtcattcctc ctaaaagata atgaggtact gtattca
//
LOCUS       DAONVQ010000203         1048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_93975_length_1048_cov_3.063444, whole genome shotgun sequence.
ACCESSION   DAONVQ010000203 DAONVQ010000000
VERSION     DAONVQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93975_length_1048_cov_3.063444"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..767
                     /gene="ftsY"
                     /locus_tag="PLD98_09045"
                     /pseudo
     CDS             1..767
                     /gene="ftsY"
                     /locus_tag="PLD98_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
BASE COUNT          333 a          221 c          285 g          209 t
ORIGIN      
        1 ttatgggcga tgtgggagtc acaaccgcgg ttgagattac acagaacctc agagaaagag
       61 ccaaaaagaa aaggcttgat aatccgcagc agataaaaga tgaaataaaa tccatcgttg
      121 ccgaaatgct tgaaggcggc gaggatatgg gacttgtcac ggttccttca attatccttg
      181 taatcggcgt aaacggcgtg ggcaagacaa caacaatcgg caaaatggca gctatgtata
      241 aggctcaagg caaaaaggtc attttagccg ctgccgatac cttcagagcc gccgcaatag
      301 accagcttca ggtctgggca gacagggcag gtgttgacat cgtaaagcac aaagaaggcg
      361 ccgaccccgc cgcggttata ttcgatacga tttccgccgc aaaggcaagg gaatcggata
      421 tcattatctg cgacaccgcg ggcagactgc acaataaaaa gaacctaatg gacgagcttg
      481 ccaagatata cagggtcatt gacagggaac ttccttattc cgacagagag gtacttcttg
      541 ttcttgacgc gactacaggt caaaacgccg taaatcaggc aagggagttt atgtctgtcg
      601 cggagcttac gggaataatc ctcacaaagc ttgacggtac cgcaagaggc ggcgttgttc
      661 tttccattaa aaacgagctg aaaatacccg ttaaattcgt cggcgtcggc gaaaagatag
      721 atgatttgca gccctttgac cccaaggcat ttgccgaggg attgtttgaa agtgttaccg
      781 agagcgaatt cccagaggat tacgcattgg cggaggctga aaattatgag gattcggcga
      841 tttatactgc ggaagctgaa gaaatacctg aagaagcagg ggctgatgat aaggacattc
      901 ccgaggacga acccgctgca gcagtcgaag aagaacccgc tgacattgac acagcagatt
      961 ctgaaggaaa tgacgaatat acacagagcg aagagtcagt ggtagaagag gaagaagtaa
     1021 aagaggataa aaagcgcaga aaaggtct
//
LOCUS       DAONVQ010000204         1035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Clostridiales bacterium isolate AMR_MDS_4196
            NODE_95758_length_1035_cov_3.206122, whole genome shotgun sequence.
ACCESSION   DAONVQ010000204 DAONVQ010000000
VERSION     DAONVQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866167
            Sequence Read Archive: SRR5007349
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridiales bacterium (wastewater metagenome)
  ORGANISM  Clostridiales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 05:43:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,810
            CDSs (total)                      :: 1,765
            Genes (coding)                    :: 1,753
            CDSs (with protein)               :: 1,753
            Genes (RNA)                       :: 45
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Clostridiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95758_length_1035_cov_3.206122"
                     /isolate="AMR_MDS_4196"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1898207"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>1035
                     /locus_tag="PLD98_09050"
     CDS             36..>1035
                     /locus_tag="PLD98_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQA06427.1"
                     /translation="MIKSGARLFENTVIGANAVIGENAIIKNNVRIWPEKQVGEGAEV
                     IDNIRDGNVTRDVFGDEGIGGITFSELSCEKAARLGCAVGSSKNGRRVCIAHDGKAAS
                     LAIEKAITAGLMSTGARVLGFGDSFVSELKFFTGYCDLKSGIFIRTENDYTEIMLFGE
                     LGLPLTRKAEREISDKYRRCDFIRCTQDSCADVVDISAIKGSYRRELEKSAFSSLDGV
                     GAKVSSDNEKIKLVINNCLNNLKLSEKDYPVFIIDSTGTEVYAKDENGEEISYETLLC
                     LAAYDELSQGKDIVISSEAPMVIDTLAKDFGVNVRRYISSSPEFEDEEMRATAEKNK"
BASE COUNT          294 a          225 c          279 g          237 t
ORIGIN      
        1 catatcttaa ttcctcggtc gtatgcgaag gcgctgtgat aaagagcggc gcaaggctgt
       61 ttgaaaatac cgtcatcgga gctaatgccg ttatcggcga gaatgcgata attaagaaca
      121 atgtacgaat ttggcccgaa aagcaggttg gcgaaggcgc agaagtcatt gacaatataa
      181 gagacggtaa tgtcaccaga gatgttttcg gagacgaggg tattggcggc ataaccttct
      241 ctgagctcag ctgcgaaaag gcggcaagac tcggctgcgc cgttggcagc tccaagaacg
      301 gcagaagggt atgcattgca cacgacggaa aggccgcgtc ccttgcaatt gaaaaggcta
      361 ttacagcggg gcttatgtcg acaggcgcaa gggttctggg gttcggtgac tcctttgttt
      421 cggagcttaa attctttaca ggctactgtg acctgaaatc gggcattttt atccgcacgg
      481 agaacgacta taccgaaata atgctctttg gcgagctggg tctgcccctt acaagaaagg
      541 cggagaggga aatcagcgac aaatacaggc gctgcgattt tatccgctgc actcaggaca
      601 gctgcgccga tgtggttgat atatccgcca ttaaaggcag ttatcgcagg gagcttgaaa
      661 aaagcgcctt ctcaagcctt gacggtgtcg gcgccaaggt aagctctgac aacgaaaaaa
      721 taaagctcgt tataaacaac tgcctgaata atttaaaact ttcagagaaa gactatcctg
      781 tgtttattat cgattctacg ggtacagaag tttacgcgaa ggatgaaaac ggcgaggaga
      841 tttcatacga aacacttctc tgccttgctg catatgatga gctgtcacag ggaaaggata
      901 tcgttatttc ctccgaggct cccatggtga tagacactct cgcaaaggat ttcggcgtaa
      961 atgtaaggcg ttatataagc agctcgcccg aattcgagga cgaagaaatg cgcgctaccg
     1021 ccgagaagaa caaat
//
